LOCUS       DASTKM010000001         4364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_704527, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000001 DASTKM010000000
VERSION     DASTKM010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704527"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1218)
                     /locus_tag="VFQ10_00005"
     CDS             complement(154..1218)
                     /locus_tag="VFQ10_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963747.1"
                     /translation="MRRLEIVQLGLGHVGRAVAQIVLEERKSWLERHGIEVRYRAVSD
                     TSGALVGEELLPQALRLKEEGGRLADLGVEPLEGVLGSEPEPGFTRIIVDVAVHGGTF
                     DLDLLGIRGGAYLVLSNKGPLSGSTAQYRQLMRMIPDRLWHEATVGAGMPIISTIGGL
                     LETGDEILEIQASPSGTLGFVMSAVEGGRSFSEAVREAVDLHYAEPDPRDDLSGLDVA
                     RKAIILARMMGREIEPHEIPYESLVPEGLEDVPLTEFMERLPEHDEAFAARLLAVEKY
                     HMLRYLVRIPKEGPVTVGLQDTPVESPFGPISGPENVFDFRTRRYSDVTLTVRGPGAG
                     PHRTASGVVWDLLDIAHKAL"
     gene            complement(1215..2441)
                     /locus_tag="VFQ10_00010"
     CDS             complement(1215..2441)
                     /locus_tag="VFQ10_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET9963748.1"
                     /translation="MERPLVIKFGGSSVGGGAQFVRAARIAADAAADNPVAVVVSAMS
                     GTTDTLLGYARMSSTWVKRTVEDETHRASIADLRRMLAERHLHAAREAVAAEHLPEVE
                     GRVVDLLGELEEVIGAPSDNVKARRDEIAVYGERLSAEILAGAISSQGALARVAAADP
                     IATDTRFGEAEVDPAATRERSARYVSPLLEEGLVAVVPGYVGHSPGGLPTTLGRGGSD
                     LSATVLGRAIDSDEVWILSDVDGVLDADPRLVPDASLMPRLSYREAGIFAGLGAKVLH
                     PRTMEPASEAGIEVFVRNTFAPDGAGTHVTAFDPGAGVRCVALRQNVAMEIPCTNGRE
                     GRAAMVVCIGSPDEEDLERGRRCLREAGIPVQHFGIAAAGLVFLVSADVGLEALRALH
                     AGLIPPAGDFAGEEVA"
     gene            complement(2447..3172)
                     /locus_tag="VFQ10_00015"
     CDS             complement(2447..3172)
                     /locus_tag="VFQ10_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET9963749.1"
                     /translation="MPSPYLVNPLEHGADIVVHSATKFIGGHGTSIGGVIVDGGTFPW
                     DNGKFPGFTEPDPSYHGLEIYPTLGELSFILKARVQMLRDWGPALSPFNSFLFLQGLE
                     TLPLRMERHSQNAQAVAEFLQGHPKVNWVNYPGLPDHPSHELAAKYHRGGLYGAILGF
                     GIEGGLEAGKRFINGLELHSLLANVGDAKSLVIHPASTTHSQLSIDEQKSTGVTDDYV
                     RLSVGLESIDDILYDLDTALNGA"
     gene            complement(3064..3753)
                     /locus_tag="VFQ10_00020"
     CDS             complement(3064..3753)
                     /locus_tag="VFQ10_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET9963750.1"
                     /translation="MTDTADQKIQERDYGFDTLALHAGQEVDSATTARAVPIYQSTSY
                     VFHDTDHAADLFSLAEAGNIYTRIMNPTTDVFEKRVAALEKGVGALATASGQAAQGLA
                     ILNIMGAGDEIVSAAELYGGTYNQFHYTLPKVGIDVKFADSTDLDGIRAAITERTKAV
                     YAETVGNPALNTLDIEGLRQWRTTPASRSLSTTRCHRPIWSTPWSMGPTSSSTLRPSS
                     SAGTARPSAVS"
     gene            complement(3787..>4364)
                     /gene="metX"
                     /locus_tag="VFQ10_00025"
     CDS             complement(3787..>4364)
                     /gene="metX"
                     /locus_tag="VFQ10_00025"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564073.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HET9963751.1"
                     /translation="LGPQGVGMSEIGRRAIMADPDWQGGDYYATGRRPDRGLAIARMA
                     GMMTYQSAAGQWERFGRRPAGRPALHPEFGGRFEIESYLHYQGRELTGRFDANSYLYL
                     SRAMDLYDLASGYESDKEAYSRIQAEVLFVGITSDWLFPAAEVRAAAESAKMAGARAH
                     YAQIDTLSGHDAFLKDWKELTEAIQPFLDKT"
BASE COUNT          730 a         1442 c         1383 g          809 t
ORIGIN      
        1 tcctcgcgct cctggccagg atgcgcaact ctcgtagcgg gaagtccctg cgcctcagct
       61 cggcgaccag cctatcccca acgagcccag ccccgactac cgctaccgta tacccatcac
      121 ccataaaacc ctccacaata aactacccga cacctacaac gccttgtgcg cgatatcaag
      181 caggtcccag accacgccac tcgccgtgcg gtgtggtccc gcgcccggtc cgcgcacggt
      241 gagggtaaca tccgagtagc ggcgcgtgcg gaagtcgaag acgttctcgg ggccgcttat
      301 gggtccgaaa gggctctcca cgggcgtatc ttgcagcccg accgtgacgg gcccctcttt
      361 cggaatacgg accaggtagc gcagcatgtg gtatttttcg acagcgagga gacgtgcggc
      421 gaaagcctcg tcgtgttccg ggagtcgctc catgaactcc gtcagcggga cgtcttccag
      481 gccttcaggg accagtgact cgtatggtat ctcgtgtggt tcgatctccc ttcccatcat
      541 gcgcgccagg atgatggctt tgcgggccac gtcgagcccc gagaggtcgt ccctcgggtc
      601 gggctcggcg tagtggagat ccaccgcctc ccttaccgct tcggagaagg atcggccgcc
      661 ctcgaccgcg ctcatgacga agcccagagt gccgctcggg cttgcctgta tctccagaat
      721 ctcatccccc gtctccagca agccacctat ggtcgagatt atgggcatgc ctgcccctac
      781 cgtggcctca tgccagagac ggtccgggat catgcgcatg agctggcggt actgggccgt
      841 gctgcccgag agcggtcctt tgttggagag cacgagataa gccccgcccc ttataccgag
      901 cagatctagg tcgaaggtac cgccgtgcac ggccacatcg acgattatcc ttgtgaagcc
      961 tggctcgggc tcggagccga gcacgccttc cagcggctcg acgccaaggt cggcgagcct
     1021 gccgccttcc tccttgaggc gtagcgcctg cgggagcaac tcctcaccga ccagcgcacc
     1081 cgaggtatcc gagacagcgc gatacctcac ctcgataccg tggcgttcga gccagctttt
     1141 gcgctcctcc agaacgatct gggcgacggc gcgcccaacg tgtcccaggc cgagctggac
     1201 tatctccagc cgcctcacgc tacctcttcg ccggcgaagt ctcctgcggg tgggatcagg
     1261 cccgcgtgca acgctcgcag cgcctcgagg cccacgtccg cggacacgag gaacacgagc
     1321 cctgcggccg cgatgccgaa gtgctgtacg gggatgcccg cctcacgcag gcaacgccgg
     1381 ccgcgctcga gatcctcttc atccggcgag ccgatgcaga cgaccatagc tgcccgtccc
     1441 tcgcgtccgt tcgtacacgg gatctccatg gccacgttct gcctgagcgc gacgcagcgg
     1501 acgcctgccc caggatcgaa ggccgtaacg tgtgtgcccg cgccatccgg cgcgaacgtg
     1561 ttgcgcacga atacctcgat gccggcctcg gaggcaggct ccatggtcct cggatggagc
     1621 accttggcgc cgagtccggc gaagatacca gcttccctgt aagagaggcg cggcattagg
     1681 gatgcgtcgg ggacgagtcg tgggtctgcg tcgagcacgc cgtccacgtc ggacaggatc
     1741 cagacctcat cagagtcgat cgcacgcccc agcacagttg cagagaggtc agagcccccc
     1801 ctgccaagcg tggtaggtag cccccctggg gagtgtccta cgtaaccagg cacgaccgcg
     1861 acaagtccct cttcgagcag tggcgagacg tagcgcgccg agcgttcgcg cgtggctgcc
     1921 gggtccacct cggcctctcc gaagcgtgta tccgtggcta tggggtcggc ggcggcaacc
     1981 ctggccagcg cgccctggct gctgatggcc cctgccagga tctccgctga caggcgctct
     2041 ccgtagacgg cgatctcatc ccggcgcgcc ttcacgttgt cggaaggagc cccgatgacc
     2101 tcttcgagct cgccgagcag gtcgaccaca cgtccctcta cctcaggcag gtgctcggcg
     2161 gccacggcct cgcgggcggc gtgcaggtgg cgctcggcca gcatgcggcg gagatcagct
     2221 atggaggccc tatgcgtctc gtcctccacg gtccgcttta cccaggtcga gctcatcctg
     2281 gcgtaaccga gcagggtgtc tgtcgtgccg ctcatcgcag agacgaccac ggccacggga
     2341 ttatccgcag cagcatcggc ggcgatacgg gcagcccgga cgaattgcgc accgcctcct
     2401 accgagctgc ccccgaactt gataaccaga ggtctctcca aaagggtcac gctccgttca
     2461 gcgccgtgtc gagatcgtac agtatgtcgt cgatagactc gaggccgacg gagagcctga
     2521 cgtagtcgtc cgtgacgccg gtggacttct gctcgtcgat cgagagctgc gagtgggtcg
     2581 tggaggccgg gtgtatgacc aggctcttcg cgtcgccgac gttcgccagg agactgtgca
     2641 gctcgagtcc gtttatgaag cgcttgcccg cttcgaggcc gccttcgatg ccgaagccga
     2701 ggatcgcacc gtacagcccg cccctgtggt acttcgccgc cagctcatgc gaaggatggt
     2761 cggggaggcc agggtagttt acccagttga ccttggggtg gccctgcagg aactcggcga
     2821 cggcctgcgc attctgcgag tggcgctcca tccgcaaggg cagcgtctcg aggccctgca
     2881 ggaagaggaa gctgttgaac gggcttaggg ctgggcccca gtccctgagc atctggacgc
     2941 gggccttcag gataaacgaa agctcgccga gcgtggggta gatctccagc ccgtggtaag
     3001 agggatctgg ttcggtgaac ccgggaaact tcccgttgtc ccaggggaag gtgccgccgt
     3061 ccactatgac accgccgatg gacgtgccgt gcccgccgat gaacttggtc gcagagtgga
     3121 cgacgatgtc ggccccatgc tccagggggt tgaccagata gggcgatggc agcgtgttgt
     3181 cgataatgag cgggacgccg gcgtcgtgcg ccactgccgc agcccctcga tatccagggt
     3241 gttgagcgcg gggttcccta cagtctcggc atacacggcc ttagtcctct ccgtgatcgc
     3301 cgcgcgtata ccgtcgagat ccgtcgagtc ggcgaacttc acgtcgatac caactttggg
     3361 cagcgtgtag tggaactggt tgtaggtgcc gccgtagagc tccgctgcgg agacgatctc
     3421 atcgccagcc cccataatgt tcaggatcgc gagaccctgg gcagcctggc ccgaggcagt
     3481 agcaagggca cccacaccct tctccagcgc ggcgactcgc ttctcgaaga cgtcggtcgt
     3541 tgggttcatg atcctggtgt aaatgttgcc cgcttcggcg agcgagaaga ggtccgccgc
     3601 gtggtcggta tcgtggaaga cgtaggaggt cgactggtag atgggtactg cccgcgccgt
     3661 cgtggccgag tcgacctcct gcccggcgtg caaagccagg gtgtcgaacc cgtagtcgcg
     3721 ctcctggatc ttctggtctg ctgtgtcggt cattgtcgct cccttcggtt gctcaggcta
     3781 gacgtatcag gttttgtcta agaacggttg tatcgcttct gtgagctctt tccagtcctt
     3841 caagaaggcg tcatggccac ttagtgtatc tatctgggcg tagtgtgccc gggcgccggc
     3901 catcttcgca ctctcggcgg cggctcgtac ttcggcggcg gggaagagcc agtcgctggt
     3961 gatcccaacg aagagcacct cggcctggat gcgcgagtag gcctctttgt cggactcgta
     4021 gccgcttgcc aagtcgtaga gatccatcgc ccttgagagg tagaggtagg agttggcgtc
     4081 gaagcggccg gtgagctccc tgccctggta gtgcaggtag ctctcgatct cgaatctccc
     4141 accgaactcg gggtgcagcg cggggcgtcc cgcgggcctg cgaccgaacc tctcccactg
     4201 cccggcggcg ctctggtagg tcatcatccc cgccatcctg gcgatggcga gccccctatc
     4261 gggcctcctt cccgtggcgt agtagtcgcc gccttgccaa tcagggtcgg ccatgatagc
     4321 gcggcgtccg atctcgctca tccccacccc ctgcggcccg agcg
//
LOCUS       DASTKM010000002         4413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_880680, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000002 DASTKM010000000
VERSION     DASTKM010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880680"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFQ10_00030"
     CDS             <1..215
                     /locus_tag="VFQ10_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963752.1"
                     /translation="GTLAGFGYDLTLEELRLVEDLRRRTAAMSDEQLARTLASGLRER
                     TGTPPARPAAPSWRGSGPGRPARPGG"
     gene            299..544
                     /locus_tag="VFQ10_00035"
     CDS             299..544
                     /locus_tag="VFQ10_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963753.1"
                     /translation="MAKTYQLDTMASPSSLLARAKKAAYANGATLLGDEHSGRFSHEM
                     AKGEYRMVGQKVIVTVTDKHWLLPWPVVEAQLRELVL"
     gene            complement(822..1247)
                     /locus_tag="VFQ10_00040"
     CDS             complement(822..1247)
                     /locus_tag="VFQ10_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9963754.1"
                     /translation="MEIVELCSAAERREALPILRELYASLDEDRYAGLLAEMVVDGYR
                     QFALRNETAEIVALAGVAVHVNLYYGRHLYVYDLVVKEEARSKGYGGLLMDHVEGVAR
                     REGCETAALACDLEREGALRLYERRGYQKPSYAMRKALR"
     gene            complement(1634..1867)
                     /locus_tag="VFQ10_00045"
     CDS             complement(1634..1867)
                     /locus_tag="VFQ10_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963755.1"
                     /translation="MLRSGRTPAKRRRVDRDRLLRDLRWLAEGNPRYAGTEEEGRQNS
                     ELAKLMAEDLLLSYIGDGEVIEVFNEIRRGHSP"
     gene            1952..2653
                     /locus_tag="VFQ10_00050"
     CDS             1952..2653
                     /locus_tag="VFQ10_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963756.1"
                     /translation="MPTNSLHEQGRLWSRPGRTPEGRSPVGLRSLRSDAAGSAEGYLY
                     VPAGYRTETPAPLVLLLHGAGEDARDGLAQLRGQADEPGIILLALSSRGPTWDSILDR
                     GRYGRDIAAIDRGLEHTFSRCAVDPTRVAVGGYSDGASYALSLGIANGDLFSHVLAFS
                     PGFVAPTGQRGSPRIFVSHGTQDRWLPIDSCSRRIVPQLERAGYEVRYREFEGGHVVP
                     PAIAREAAIWFTNRN"
     gene            complement(2712..3260)
                     /locus_tag="VFQ10_00055"
     CDS             complement(2712..3260)
                     /locus_tag="VFQ10_00055"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HET9963757.1"
                     /translation="MEESKHGSAPTLPKAALAYARRGVPVFPCEPGAKRPLTRNGHWD
                     ATTDPRVIERWWKRWPSANVGVPTGKKSGVVVLDVDVDDGGLESLAKLERAGASAPKT
                     ARARTGGGGIHVFFRNPRGTQIRNSAGLLGPGLDVRGQGGYVVVPPSRTEDSYKWVDR
                     SPLAEASWLIERLTEREEATLF"
     gene            3641..4402
                     /locus_tag="VFQ10_00060"
     CDS             3641..4402
                     /locus_tag="VFQ10_00060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9963758.1"
                     /translation="MSPVAIVTASDSGIGQESAKELAENGFDLGITYRTDEAGAQETL
                     EAVRAAGRRGEVRRLDLTELPGAADVIDVLADALGGMDVLVNNAGTGDPTGAFLELSY
                     EEWQQVIDTNLSGAFLCAQRAARRMVDAGNGGRIINITSVHEHIPRTGASAYCSSKGG
                     LGLLTKVMAMELAEYGILVNAVGPGEISTPMTGAEDEDPTTIERPKLPLGRPGHAREI
                     AAWVAFLASEKSSYATGASFVVDGGLKLMAAELSS"
BASE COUNT          789 a         1427 c         1391 g          806 t
ORIGIN      
        1 aaggcacgct tgcagggttc ggctacgacc tcacactcga ggagctgcgt ttggtggagg
       61 acctcaggcg tcggactgcc gcgatgagcg acgagcaact cgcccgtacg cttgccagtg
      121 gcctccgaga gcgtaccggc accccgccgg cccgacctgc tgccccgagt tggcgcgggt
      181 cgggtccggg tagaccggca cgacccggag gttgacggtg gcgggtatgt gtaacggcca
      241 caggacatgc agggtctagt cgttggagca ttcccaccag tatggaagga agtagatagt
      301 ggcgaagacc taccaactag acacgatggc aagcccctcg tcgctgctgg cgagggccaa
      361 gaaggccgcc tacgcgaacg gcgccaccct gttaggcgac gagcactcgg ggcgcttttc
      421 gcacgagatg gccaagggcg agtaccgcat ggtggggcaa aaggtgatcg tcaccgtgac
      481 cgataaacac tggttgctgc cctggcctgt ggtagaggcc cagctacgag agctcgtact
      541 ctagcctccc tatcgcttgt gtggaaagcg gcgcgaggcc cgcgaggagg cggagtcagt
      601 atgggcgacc aacgtgtatg gtgcgtatgc ccccagcaca tcatgagccg ccacctaatg
      661 tgaggttctt atgtgctttc acgggccgaa actcaacggg cgtaagctct ctggctacca
      721 gacactgagg cgtcccgacg tgctagtaca gactgcgctc tgcgatcagc cgtgctctcg
      781 gtattccaag cgattcttcg caaaactcga acgccgctct ctcaacgcaa cgccttgcgc
      841 atcgcataac tgggcttctg atagcccctg cgctcgtaga ggcgcagcgc cccctcgcgt
      901 tccaagtcgc aggccagcgc agccgtctcg cacccttcgc ggcgggcgac gccttccacg
      961 tggtccataa gcaatccccc gtatcccttg gacctcgcct cctccttaac cactagatcg
     1021 tagacgtaga ggtgacgccc atagtagagg ttgacgtgga cggccacccc cgccaaggcg
     1081 acgatctctg cggtctcgtt acgcagcgcg aactgacggt atccgtcgac caccatctcg
     1141 gctagcaaac ccgcatagcg atcttcgtcc agcgaagcgt acagttcgcg gagtatcggc
     1201 agtgcttctc tacgctctgc tgccgagcac agttccacta tctccatcgc tttcctcctc
     1261 ttgggcgttg cgagttttcc agaaccacct cagattatgc tctcttagcc tacctgcgta
     1321 tcgctagacc ccctggcgga gccattgatc tacgtgagcg gatgagctct tgcggagcaa
     1381 ctacccgacc tcaagcagct cagaatggct cgcgaggtcc acgaggagac agaagcggga
     1441 cggaatatcg ttaccgcctt ctcctgagtc gaagccagtt gagacaacgg cgggattgag
     1501 cttccgggtc ttgttacact tcagagcggt ttcgcaccag gtcgttgaca cgccacgacc
     1561 cgtcagttct ccgaggatct ggacacgcgc ccgacgtggg cgttcgcccg accctaccca
     1621 acagacataa ccactagggc gaatgacctc ggcgtatctc gttgaagacc tcgataacct
     1681 ccccgtcacc tatgtagctc aggaggagat cttcggccat cagcttggcg agttcggagt
     1741 tttgcctgcc ctcctcctcg gtgcccgcgt agcgcgggtt gccctcagct agccacctca
     1801 ggtctctcag cagcctatct cggtctacgc gtctcctttt ggccggcgtt cgccccgatc
     1861 gtagcatcgc cgtcccggcc atcccgcggc ggtggcccgt ctcctaggta gaagagtgta
     1921 agtaagagca ggccggggaa ttgatgggcg catgccgacc aacagcctac atgagcaagg
     1981 gcgcctgtgg tcgcgcccgg ggcggacgcc cgaggggagg tctccggtcg ggctgcggtc
     2041 gctgagatcc gacgccgccg gctctgccga gggctacctc tacgtccctg ccggctaccg
     2101 gacggagacc cctgccccgc tggttctcct gctgcacggt gccggagagg atgcgcgcga
     2161 cggcttggcg cagctcaggg gacaggcgga tgagccgggg atcatcctgc tcgcgttgag
     2221 ttcgcgcggg cctacttggg actcaatcct cgaccgaggg cggtacggta gggacatcgc
     2281 agccattgat cgggggctgg agcacacctt ttcccgctgc gctgtcgatc cgacgcgcgt
     2341 ggcggtcgga ggttactcgg acggagcctc ctacgccctc tcgctaggga tagcgaacgg
     2401 cgacctgttc tcgcacgtgc tcgccttctc gccggggttc gtggctccga ccggccagag
     2461 gggctcgccg cgtatctttg tctcgcacgg gacccaggac aggtggctcc cgatcgactc
     2521 gtgcagccgc aggatcgtcc cccaactaga acgcgctgga tatgaggtac gctaccgcga
     2581 gttcgaaggt ggccacgtgg ttcctcccgc catcgcgcgg gaggcagcca tctggtttac
     2641 caatcgaaac tgaagacgca ccgactgatc gttctcgtgc aaccggggtc ctagaggggg
     2701 acgaacaccg ctcagaacag cgtcgcctcc tctctctcgg tcaggcgctc tatcagccac
     2761 gatgcttcag cgagaggcga acggtcgacc cacttgtaag agtcctccgt ccggctaggc
     2821 ggcaccacga cgtagccgcc ctggcccctc acgtcgagcc ccggaccgag cagtcccgcg
     2881 ctgttgcgaa tctgcgtgcc cctcggattg cggaagaaga cgtgaatgcc gcctccccca
     2941 gtgcgcgccc tagccgtctt cggggccgaa gctccagcac gctcaagctt cgcgaggctt
     3001 tctagcccac cgtcgtcgac gtccacgtcc agaaccacga cgccgctctt cttaccggtt
     3061 ggaactccca cgttggcaga gggccaccgc ttccaccatc gctcgatcac gcgcgggtcg
     3121 gtggtcgcgt cccagtggcc gttacgcgtc agcggcctct tggcgcctgg ttcgcagggg
     3181 aacacgggca cgccgcgcct ggcgtaggcc agagccgcct tgggcagcgt aggagccgat
     3241 ccatgcttac tttcttccaa cctgctccga ttctacaggt ctgacgccgc ttcgggcgcc
     3301 tccctgcacc cttgtgtgta gaagaagggc cagcgggctc ttctgtcgca accgaccccg
     3361 cgtcggccaa cgccggtggc gacaacggtg ctgccgtttc gcattggggt ggcggtcgat
     3421 ggcgaggtaa agatgagact cgccgcttag cgacgcccac gaggaagcta tgcgcagggg
     3481 ttggctcaag gacgcctggg gaaggccgcg ctgccgcacg ctctatcgcg cctctgtaga
     3541 taccgacgac ttcgactcgt aaggacccta ctctgtttgt gctctccact ccgggggcat
     3601 agagccgggt gggtaagcct tgcggacgag aggagggggt atgagtcccg tagccatagt
     3661 taccgcatcg gactcgggga tcgggcagga gtcggcgaag gagctggcgg agaacggctt
     3721 cgacctcggc atcacctacc gtacggacga agcaggggcg caggagaccc ttgaggccgt
     3781 gcgggcggcg ggccgcaggg gcgaggtgcg aaggctggac ctcacggagc tgccgggggc
     3841 tgcggacgtg atcgacgttc tggccgacgc tctgggcggc atggacgtgc tggtcaacaa
     3901 cgccggaacc ggcgatccga caggcgcgtt cctggagctc tcttacgagg agtggcagca
     3961 ggtcatagac accaacctct ccggggcttt cctgtgtgct cagcgggcag cgaggagaat
     4021 ggtcgacgcc ggaaacgggg gcaggatcat caatatcacc tccgtgcacg aacacatccc
     4081 gcgcaccggt gcttcagcgt actgctcctc caagggtggt ctcgggctcc ttaccaaggt
     4141 catggcgatg gagctggcgg agtacggcat cctggtaaac gcggtcggac ctggtgagat
     4201 ctccacgccc atgaccggcg ccgaagacga ggatcccacg accatcgagc gcccgaagtt
     4261 gccactagga cgccccggac acgcccgcga gatcgcagcg tgggtggcct tcctcgcctc
     4321 ggagaagtca tcctacgcaa ccggcgcctc attcgttgtg gacggcggcc tgaagctgat
     4381 ggctgcagag ttgtcgagct aggctaccag aac
//
LOCUS       DASTKM010000003         5580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1056884, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000003 DASTKM010000000
VERSION     DASTKM010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056884"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..2047
                     /locus_tag="VFQ10_00065"
     CDS             65..2047
                     /locus_tag="VFQ10_00065"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HET9963759.1"
                     /translation="MTETAEGTPLWEPSEELKENARISDYMEWLKAEKDLSFGNYDEL
                     WEWSVTDLEGFWATVWEYCGIKASKPYERVLGKREMPGAEWFPGAELNYAEHVLRHAA
                     HRPDEPAILHQSEVRPLGEVSWGELQEKTAALAAGLREMGVGRGDRVAAYLPNIPEAV
                     IALLACASIGAVWSSCSPDFGAGSVVDRFKQIEPKVLLAVDGYRYGGKDYDRIEVVAR
                     LQEEISTLEKTIVLPYLSADPDTSALESVVVWDDLLAGHEGAELAFEQVPFDHPLWVL
                     YSSGTTGLPKAIVHSQGGILIEHLKKAVLHLDLGPDDRFFWFTTTGWMMWNLVVAGLL
                     TGSTVLLYDGNPGHPDMDVLWEFAEKTRMTCFGTSASYITACMKADIEPGRDFDLTNL
                     KSIGSTGSPLPPEGFEWAYAHVKEDLWLFSTSGGTDLCSAFVGGVPLLPVRAGELQAR
                     SLGAKVEAFDEEGNPQVDDVGELVITEPMPSMPIYFWNDPEGERYRESYFDVYPGVWR
                     HGDWIKVKENGACVIYGRSDSTINRGGIRMGTSEIYSAVDKLEEVADSLVVDVPREGG
                     GSFMPLFVVLQEGVEFDDDLKNTIKGSIKENTSPRHVPNEIFAVPEIPKTLNGKKLEV
                     PVKKILSGTPPEEAASKESLSNPESLDHYVELAREM"
     gene            2220..3005
                     /locus_tag="VFQ10_00070"
     CDS             2220..3005
                     /locus_tag="VFQ10_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9963760.1"
                     /translation="MYETLLYEEDNGVANIALNRPEKLNAFDGKMHEDLYRALGSVTE
                     DEEIRCIVLRGEGRGFSAGADLAQVVREADGDPDLGEYLRATYSRLVKRMTGIEKPIV
                     AALHGPVYGAGVGIALACDLRVAAQSAKFSVAFVKIGLMPDAGVTFLLPRVVGLGRAM
                     EMSMLGDAIDAEEAHRIGLVNKVVADDSLWQEARNLADRLAAMPTVAMGRIKHAHYAT
                     FETDLETTLEAEAEGQTFCGYTQDHKEGVTAFFEKREPKFTGR"
     gene            complement(3027..3455)
                     /locus_tag="VFQ10_00075"
     CDS             complement(3027..3455)
                     /locus_tag="VFQ10_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963761.1"
                     /translation="MSDLEQHREGDRKGNPPLFLSLGIGFVIAAILVIVTSGTAAAGL
                     PFVIVAAGLLGKGLSDMRGQQRAVGPPVSTERELLSAIKANGGSITPAEAAMETSLTV
                     READGVLSELAGGGHLAVESRDGALFYSLQGRRNPELDGR"
     gene            3621..4427
                     /locus_tag="VFQ10_00080"
     CDS             3621..4427
                     /locus_tag="VFQ10_00080"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HET9963762.1"
                     /translation="MHETGVGDFLKRLRLLMTRQEIVPERLAGATAVVFDVFMATTTL
                     LTILENGARDVYPASSLEEADEVGGRLDTGSFLRGGEQDAARIEGYDHGPFPEEYAPE
                     VVRGTDVIFVTTNGTRAIADVAPADTVLLGTLRNSGAVAHHLETSMTDSIYLVCAGAG
                     GRFNVEDFLGAATVLSHMDVDGFRLNDGALLALDFAERHEENVYETLKRSRAGRWFVE
                     NDRMDAFDFVADTGASGLVPEVVGGKLRRANEHESYVGDAEWGRILGERP"
     gene            4424..5482
                     /locus_tag="VFQ10_00085"
     CDS             4424..5482
                     /locus_tag="VFQ10_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET9963763.1"
                     /translation="MSETIEIRQGEAFDLEAAERYLRAHVEDVPEGDLEVSQFPSGAS
                     NLTYLLKVGHWEGVLRRPPLGPIPPKAHDMGRESGILARLNAVYPLAPKPYFLCEDES
                     VIGAPFYVMERRTGIVLDDSFPDGVEHDEGLCHGISRTLVDTLVELHAVDVREAELGD
                     LGKPDGFLQRQTEGWISRYERAKTDEVEEVGPLTDWLARGIPESPPPAVIHNDYKLNN
                     LVLDPEDLTDVRAVLDWEMATVGDPLFDLAVSLSYWIEPGDPDELVAVMPTVTVTPGF
                     MTRKELIDRYVEQSGRDLSEMHWYVVFGYFKLAGILQQIYARYKNGQTTDERFATFGE
                     RVRTLILHAENLSRTGDV"
BASE COUNT         1126 a         1717 c         1790 g          947 t
ORIGIN      
        1 cgtccctcgt catggacggc ggggcgacgg tccactagag agctcttatc agaaaggaac
       61 tatcgtgacc gaaaccgcgg aagggacccc gctatgggag ccttcggaag agctcaagga
      121 gaacgccagg atctccgact acatggagtg gctgaaagcc gagaaggacc tctctttcgg
      181 caactatgac gagctctggg agtggtctgt cacagacctc gaaggattct gggccactgt
      241 gtgggagtac tgcggcatca aagcctcgaa gccctacgaa agggtgctcg ggaagcgcga
      301 gatgccaggc gccgagtggt ttccaggggc cgagttgaac tacgccgagc acgtcttgag
      361 gcacgctgca catcgccctg atgagcccgc gatcctgcac cagtccgagg tgagacccct
      421 gggtgaggtt agctggggcg aactgcagga gaagacggct gcgctcgccg ccggtctgcg
      481 ggagatgggc gtcgggcggg gcgacagggt cgcggcttac ctgccgaata tccccgaagc
      541 ggtaatcgcc ctgctcgcct gcgcttccat aggtgcggtc tggtcgagtt gctcccccga
      601 cttcggtgct ggcagcgtcg tcgaccgttt caaacagata gagcccaagg tcctcctcgc
      661 ggtcgacggc taccgctacg gcggcaagga ctacgacagg atagaggtcg tcgcgaggct
      721 gcaagaggag atctcgacgc tagagaaaac catcgtactc ccttatctgt cagcagatcc
      781 ggacacgagc gctctggaga gcgtcgttgt gtgggacgac ctcttggccg ggcacgaggg
      841 cgcggaactc gctttcgagc aggtgccttt cgaccatccg ctctgggtac tctactcctc
      901 tggaacgacg gggcttccga aggccatcgt gcacagccag ggtggcatcc tcatcgagca
      961 cctaaaaaag gccgtgttgc acctggacct cgggccggat gaccgctttt tctggttcac
     1021 cacgacgggg tggatgatgt ggaacctggt cgtcgctggc ctgctcaccg gctctaccgt
     1081 cttgctctac gatggcaatc ccggccaccc cgacatggac gtactttggg agttcgccga
     1141 gaagacccgc atgacctgtt tcgggaccag cgcgagttac atcacagcct gcatgaaggc
     1201 ggacatagag ccggggaggg acttcgacct gacgaacctg aagtccatag gttcgactgg
     1261 ctcgccgctg ccgcctgagg gcttcgagtg ggcctacgca cacgtcaagg aggacctctg
     1321 gctcttctcg accagcggag ggactgacct gtgcagcgcg ttcgtcggcg gggtaccgct
     1381 cctgcccgtc cgcgcgggtg agctccaggc ccggtccctc ggggcaaagg tcgaggcctt
     1441 cgacgaggag gggaacccac aggtagatga cgtcggggag ctcgtcatca cggagcccat
     1501 gccgtccatg ccgatctatt tctggaacga tccggaaggg gagcgttaca gggagagcta
     1561 cttcgatgtc taccccggcg tgtggcgcca cggagattgg atcaaggtga aggagaacgg
     1621 ggcttgcgtg atctatggtc gctccgactc gaccattaac cgcggcggca taaggatggg
     1681 gacgagcgag atctactccg ccgtcgataa gctcgaggag gtagccgata gcctcgtcgt
     1741 cgacgtcccc agagaagggg gcggctcctt tatgccgctc ttcgtggtct tgcaagaggg
     1801 cgtcgagttc gacgacgatt tgaagaacac gataaagggg agcatcaaag agaacacctc
     1861 gcccaggcac gtgcccaatg agatcttcgc cgtccccgag atacccaaga ccctcaacgg
     1921 caagaagcta gaggtgcccg taaagaagat cctctcgggc acgccgcctg aggaggccgc
     1981 gagcaaggag tctctcagca accccgaatc cctcgaccac tatgtggagc tggccaggga
     2041 gatgtaactc tatagtgaat gccaagagcg ctcctgatca cggagacctg cgaggacgag
     2101 atgatccgtg atcaggtacg ggagaccgaa gtggaggcgg ctacgacagc atagcttcgc
     2161 caactgttcc atctctgtgt gcccgccgct gcgggggcat cgttctcgaa ggagggaagt
     2221 tgtacgagac cctgctctac gaagaagata acggggtggc caacatcgcc cttaaccgcc
     2281 ctgagaagct gaacgccttc gacggcaaga tgcacgaaga cctctaccgt gccctcggta
     2341 gcgtcacgga agacgaagag atccgctgta tcgtgttgcg cggcgagggc cgcggtttct
     2401 ccgcgggcgc cgacctggcg caggtcgtga gggaggctga tggagacccc gacctcgggg
     2461 agtacctgcg tgccacatac agccggctcg taaagcggat gaccgggatc gagaagccca
     2521 tagtcgccgc cctgcacgga ccagtctacg gtgctggggt gggcatagcg ctcgcgtgcg
     2581 acctgcgcgt cgcggcacag agcgcgaagt tctcggtggc ctttgtcaag atagggctca
     2641 tgcccgacgc aggggtcacg ttcttattgc cccgcgtcgt cggcctcggc agagctatgg
     2701 agatgagcat gctcggcgac gccatcgacg cagaagaagc gcacaggata gggctcgtca
     2761 acaaagtagt agccgacgat tccctctggc aagaagcgcg gaacctggcc gaccgcctcg
     2821 ccgcgatgcc gacggtggcc atgggtagga tcaaacacgc ccactacgcc accttcgaga
     2881 cagacctcga aactaccctg gaggccgagg ccgagggtca gacgttctgc ggctacacgc
     2941 aggaccacaa agagggcgtc acagcgttct tcgagaagcg cgagccgaag ttcacgggcc
     3001 ggtaggggcg ggtttaaaac ccgcccctac cttccgtcga gctcagggtt gcgtctgcct
     3061 tgcagcgagt agaacagcgc tccgtccctg ctctccaccg cgaggtgccc gcctccagcg
     3121 agctccgaca gcaccccgtc cgcctcgcgc acggtgagcg aggtctccat cgccgcctcc
     3181 gcgggcgtta tgctgccacc gttggccttg atcgctgaaa ggagctcccg ctctgtgctg
     3241 acgggtggtc ccacggcgcg ctgctggccc cgcatgtcgg acaagccctt gccaagcaag
     3301 ccagcggcca cgatcacgaa tggtagaccc gctgcagccg tccccgacgt cacgatgacc
     3361 agaatcgcgg cgatcacgaa cccgatgccc agtgagagga agagaggcgg gttacctttg
     3421 cggtcccctt ccctgtgctg ctccagatcg ctcaccacac ctccagattc cttgcaaccc
     3481 gatccaaaac aggtcgtcaa ccccattcta gaccccacct accacagtgc aagccaggcc
     3541 ctgtgatccc agcgcgcctc ccgcgcaacc gcagagccga tgaggttctg cgcggcgtgt
     3601 ttctgtagaa tggtgcgggc ttgcacgaga cgggggtcgg cgatttcttg aagaggctca
     3661 ggttgctaat gacgcggcag gagatcgtgc ccgagagact tgcgggcgcc acggccgtcg
     3721 tcttcgacgt gttcatggcg acgacgaccc tgctgaccat cctggagaac ggcgcaaggg
     3781 acgtctaccc cgcgtcgagc ctggaggagg cagatgaggt cggggggagg ctggacaccg
     3841 gctcgttcct gcgcgggggc gagcaggacg cggcgcggat agagggctac gatcacggtc
     3901 ccttcccgga agagtacgca cctgaggtcg tcaggggcac agatgtgatc ttcgtgacca
     3961 ccaacggcac ccgcgccatc gccgatgtgg ccccggcgga tacagtcctc ctcggaaccc
     4021 tccgcaactc tggggccgta gcacaccacc tcgaaacctc gatgacggat tctatctatc
     4081 tcgtctgcgc gggcgcaggg ggccgcttca acgtcgagga cttcctcggc gcggcgacgg
     4141 tcctctcgca catggatgtg gacggctttc gcttgaacga cggcgccttg ctagctctcg
     4201 acttcgccga acgccacgaa gagaacgtct acgagacgct gaaacggagc cgggcgggtc
     4261 gatggttcgt cgagaacgac aggatggacg ccttcgactt cgtcgccgac acgggcgcga
     4321 gcgggttggt ccccgaggtc gtcggtggaa agctccgacg cgccaacgag catgaaagtt
     4381 acgtcggaga cgccgagtgg ggtcggatcc tgggagagag gccatgagcg agacgataga
     4441 gattcggcaa ggagaagcct tcgacctcga agcggccgag cgttatctgc gggcacacgt
     4501 agaggacgtg ccagaaggcg acctcgaagt cagccagttc ccctccggcg cttccaacct
     4561 gacgtacctc ctgaaggtag ggcactggga gggcgttctg cgccgcccac cgctcggacc
     4621 gatcccgccg aaggcccacg acatgggccg cgagtcgggc attctagcca ggctcaacgc
     4681 tgtctacccg ctcgccccaa aaccgtactt cctctgcgaa gatgagtccg tcataggggc
     4741 tcccttctac gtgatggaac ggagaacagg gatcgtgctc gacgactcct tccccgatgg
     4801 cgtagagcac gacgaggggc tgtgccatgg gatctcacgc accctcgtag acacgctcgt
     4861 cgaattgcac gcggtagacg tcagggaggc agaactgggg gacctcggca agcccgatgg
     4921 tttcctgcag cggcagacag aaggttggat ctcacgctac gaaagggcga agaccgacga
     4981 ggtagaagag gtcggccccc tcacggactg gctggcccgt ggcatccccg agagcccacc
     5041 tccggccgtc atccacaacg actacaagct caataacctc gtcctcgacc cggaagacct
     5101 gacagatgta cgcgcggtcc tcgactggga gatggcgacc gtcggggacc cgctcttcga
     5161 cctcgccgtc tcgctctcgt actggatcga acctggcgac cccgacgagc tcgtggccgt
     5221 catgccgacc gtcacagtaa cgccaggttt catgacccgc aaagagctca tcgatcgcta
     5281 cgttgaacag agtgggcgcg acctctccga gatgcactgg tacgtcgtct tcgggtactt
     5341 caagctggcc gggatattgc agcagatcta cgcccgctac aagaacggcc agacaacaga
     5401 cgagcgcttc gcaaccttcg gcgagcgggt aagaactttg atcctgcacg ccgaaaacct
     5461 ctcccgcacg ggggacgtct agtgcccggc gaagcccgaa gggcggagcg tgctgaaatg
     5521 ctgagcgact gaaaggagcg acacacattg ggccgtctgg acggcagggt tgcgatggtg
//
LOCUS       DASTKM010000004         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_176521, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000004 DASTKM010000000
VERSION     DASTKM010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176521"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1777
                     /locus_tag="VFQ10_00090"
     CDS             56..1777
                     /locus_tag="VFQ10_00090"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9963764.1"
                     /translation="MDEGKNFTSMEESSTPELVLLAVDDDPNALGRIEHELRQRYGSY
                     YRVACEDSPEAGMRTLRELQAAGEDVAVVLADQWMPGMTGLEFLTHTRQLYPGAKRAL
                     LFERGNRTTREPILQGMALGQIDYYVPKPERAPDEEFHRCLTQFLDEWAREHRPASVA
                     VQIVGDPWAARSHELRDLLGRSGIPQAFAAADSKEGQELLARMEKTSAQLPVVVVFDR
                     LALVDPSNAEMADAFEMISPFGLNTLPDVRNFDLVIIGAGPAGLAAAVYGASEGLRTV
                     VVERETFGGQVGTSSLIRNYLGFSRGVSGSELAWQAYQQAWLFGASFRLARQATALHH
                     RGERLVVTLSDGTEVAGRMVIVTTGVSYRRLGVPSLEALQGAGVLYGAAVTEAQGMKG
                     QEVYVVGGGNSAGQAARHLSKHARHVTLLARGESLASGMSQYLIKELEAAENIQVRVN
                     TQVVNGGGQGRLEHLVLEDCATKRTETVPAAALFVLIGARPHTRWLPEEVMRDERGYV
                     ITGNDLLRDGSPPEGWPLERAPMLLETSVPGVFAAGDVRCGSVKRVASAVGEGAIAIQ
                     MVHKYLN"
BASE COUNT          380 a          579 c          664 g          384 t
ORIGIN      
        1 gggtcgttaa cgcgtgctat ttcaacgggg tgtgagatgc tagtaaaacg tccaaatgga
       61 cgaaggcaag aacttcacga gtatggaaga atcctctact cctgagctgg tgctgctcgc
      121 tgtagacgac gaccctaatg ctctcggccg gattgagcac gagcttcgtc agcgctacgg
      181 cagctattac cgtgtcgcct gtgaggactc accagaggcg ggtatgcgga cgctgcggga
      241 gctccaggcg gctggcgagg atgtcgccgt tgtgctggcc gaccagtgga tgccagggat
      301 gacgggactt gagttcctaa cccacactcg acagctctat cccggcgcga agcgggcgct
      361 cctgttcgag cggggcaaca ggaccaccag agaacccatc ctacaaggca tggctctcgg
      421 gcagatcgac tactacgtgc ccaagccaga acgggctccg gacgaggagt ttcacaggtg
      481 cctcacgcag ttcctggacg agtgggcgag ggagcatcgc ccggcgtccg tggcggtaca
      541 gatagtcggc gacccgtggg ctgcgcgatc tcacgagctg cgggacctac tcggtcgcag
      601 cggaatcccc caggcgttcg ctgccgcaga ctcgaaggag ggccaggagc tcctggcccg
      661 aatggagaag acctcggccc agctacccgt tgtggtcgtg ttcgaccggc tggcgctcgt
      721 cgatccttct aacgccgaga tggctgacgc attcgagatg atctctccct ttggattgaa
      781 caccctccca gatgtgcgga acttcgacct cgtgatcatc ggggcagggc ccgccggcct
      841 ggctgcggcg gtctatggcg cttccgaggg cctccgcacg gtggtcgttg aaagggagac
      901 cttcggaggg caggtgggca ccagttccct gatccgcaac tacctcggtt tctctcgggg
      961 agtaagcggt tcggagctgg cctggcaggc gtaccaacag gcgtggctgt tcggagcttc
     1021 gttccgcctc gcccgccaag ccaccgcgtt gcaccataga ggagagcggt tagtcgtaac
     1081 cctctcagac ggcactgagg tggcgggtcg gatggtgatc gtcaccacgg gcgtctccta
     1141 ccgccgcctg ggtgtcccga gtctcgaagc cttgcagggc gcgggcgtgc tctacggggc
     1201 cgctgtgacc gaggcgcaag gcatgaaggg acaggaagtc tacgtggttg gcggagggaa
     1261 ctctgccggg caagcagcga ggcatctctc caaacacgcc aggcacgtta cgctgctggc
     1321 gcgcggcgag tctctcgcat ccggcatgtc tcaatacctg atcaaggagt tagaggccgc
     1381 cgagaacata caggttcgcg taaacaccca ggtggtaaac ggcggtgggc agggacgtct
     1441 ggagcacctc gtacttgagg attgcgccac gaaacgtacg gagacggttc ccgcagcggc
     1501 cttgttcgtg ctcatagggg ctcgtcctca caccaggtgg cttccggagg aggtcatgcg
     1561 cgacgagagg ggctacgtca tcaccggaaa cgatctcctg cgagacggat cgcctcccga
     1621 aggatggcct ctggagcgtg cgccgatgct cctggagacc agcgttcccg gtgtgtttgc
     1681 ggccggggac gttagatgtg ggtcggtgaa gcgggtggct tcggcggtgg gtgagggcgc
     1741 gattgcgata cagatggttc acaagtacct gaactgagtc gtgggcgcct gttatcggtt
     1801 gccacttggc cgtgacaccg ccgctgctcg tctggaaagc gcgttagata gccactccca
     1861 cgccgcatac ttcggatgat tactccaact ttacattgct atcgtgtcct gagcgggatt
     1921 gtacataagc ttactgcgat aggtgaaact cgctagctcc aagctacaga atatggatac
     1981 acccacggta gtgcaggtga ctgagcc
//
LOCUS       DASTKM010000005         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1409517, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000005 DASTKM010000000
VERSION     DASTKM010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409517"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFQ10_00095"
     CDS             complement(<1..825)
                     /locus_tag="VFQ10_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET9963765.1"
                     /translation="MERETENQFFGPNLGYVLELYENYREDPDSVDGQMREFFESWSP
                     PRVGMPGANGQQVAPEVDVEKAVGASKHVRHIRDFGHRAAKLDPLGSEPPGDPTLDPT
                     FYRITDEDLETLPSLIIGGPIAERTSNAREAAEELRRIYCDTTGYDFGHVHDPEERFW
                     LRESVESGRFDKRMEGESAKRLLQRLTRVDAFEKFLHKTFLGQKRFSVEGNDMVVPML
                     NRIIRSAADVGTPVVTLGMAHRGRLNVLAHVMDKPYSKIFGEFQQPEKGEQSSAASS"
     gene            1227..1544
                     /locus_tag="VFQ10_00100"
     CDS             1227..1544
                     /locus_tag="VFQ10_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963766.1"
                     /translation="MEDRIDYFERMLADNPDNPTGLLALANEYEKAGRDEDEAAVLER
                     YLAVQVDDEGNAYARLGSVLSNLGRKDEARAAYEKGIAQSEKHGHSGMADDLRLALEQ
                     LNE"
     gene            1834..>2160
                     /locus_tag="VFQ10_00105"
     CDS             1834..>2160
                     /locus_tag="VFQ10_00105"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9963767.1"
                     /translation="MDLGTRPTADEVKAILGLEPHPTCGFVAETYRSSLRIPGGALPE
                     AYEGDRPYGSALYFLVAPDAQIVMHRIRSDQLYHHYMGDPLEVLMLYPDGTGTVTTVG
                     SDLAAGQ"
BASE COUNT          430 a          704 c          629 g          397 t
ORIGIN      
        1 gctgctcgcg gccgaggact gctcgccctt ctcgggctgc tggaactcgc cgaagatctt
       61 ggagtaaggc ttgtccatca catgcgccag gacgttgagc ctccccctgt gcgccatgcc
      121 aagggtgacc acgggcgtgc ccacgtcggc ggcggaacgg atgatcctgt tcagcatcgg
      181 aacgaccatg tcgttcccct ccacggagaa gcgcttctgg ccgaggaacg tcttgtggag
      241 gaacttctcg aaggcgtcta cccttgttag cctctggagg agccgcttcg cgctctctcc
      301 ctccatcctc ttgtcgaagc ggcctgactc gacggactcg cgcagccaga acctctcctc
      361 agggtcgtgg acgtgcccga aatcgtatcc cgtggtgtcg cagtagatcc tgcgcagctc
      421 ctcggcggcc tccctggcgt tggacgtacg ctcggcgatg ggtccgccta tgatgagcga
      481 gggcagggtc tccaggtcct cgtccgttat cctgtagaag gtagggtcga gcgtcggatc
      541 ccctggcggc tcgctcccga gagggtcgag cttcgcggcc cgatgcccga aatcccttat
      601 gtgcctgacg tgtttcgagg ccccgacggc cttctccacg tcgacctcgg gggctacctg
      661 ctgcccgttg gcgccaggca tccccacccg cggcgggctc caggactcga agaactcccg
      721 catctgtcca tcgacggaat cggggtcctc cctgtaattc tcgtagagtt ccaacacgta
      781 gccgaggttc ggcccgaaaa actggttctc cgtctctctc tccaatcgga tcacaccctc
      841 acgtgacgat atcctctttg gcccgagcgc cgcgcgaaga ggccctatac ccttcgcggg
      901 caccccccaa aaccgctcct gcaaagagat caaacatctc gatgtttcct atgatacccc
      961 acatccccag taccgtacct acccgcatac ctacctgaat agcatgtcgg ctagtacctt
     1021 agcatgcgag gctcccacac ccagccaagc tatgtggcaa acgaggcggt gccttacgca
     1081 gagagcacca ccaacgcaag ccgctaccca aaaaggctta actgccgaca agctgaggcc
     1141 ccgggccgaa gcacatgctc gtcgggatcg ggcctgatgg caggcgctgt atactggcgt
     1201 cttcgaaatg tgaagggagg aacctcatgg aagacaggat agattatttc gagcggatgc
     1261 tggctgacaa cccagacaat ccgacggggt tgctggcact ggccaacgag tacgagaagg
     1321 ccggacgcga cgaagacgag gccgcggttc tggagcgcta cctggccgtc caagtcgatg
     1381 acgagggaaa cgcctacgct cgcctcggca gcgtgctctc caacctaggg cgcaaagatg
     1441 aggcgcgtgc cgcctacgag aaggggatcg cccagtccga gaagcacggc cacagcggga
     1501 tggcagacga tctcaggctc gcacttgaac agcttaacga gtagatcctg ttccagacga
     1561 tgcagcccgg cggcatgccg ggagcacctg ggtccataga attttccgcg ccgtaacaga
     1621 atcggtacac gggcgttacc accccttaac gtatctgcct gataatgcga gcaagctccc
     1681 cggcgaagta gaggatcccc ctccgcttgc acaacacgcc ggggagcggt cttgtggctt
     1741 cctatacgcc ctacttgagt agacttccag aagatgcacg gatatgctcc tagcgtgcag
     1801 tgaccgaaca gatgaatctc caaggaggag acgatggatt tagggacgcg tccgacggcg
     1861 gatgaggtca aggctatact cgggctcgag ccccacccca cgtgcggctt cgtcgccgag
     1921 acctaccgca gttcactgag gattcccggc ggggcgttgc ccgaagcgta cgagggcgac
     1981 cgcccgtacg gctcggccct ctacttcctc gtcgccccgg acgcccagat cgtcatgcac
     2041 aggatacgct ccgaccagct ctaccaccac tatatgggag atccgctcga ggtattgatg
     2101 ctctaccccg acggtacagg caccgtgacg accgtaggtt ccgacctggc ggcgggccaa
//
LOCUS       DASTKM010000006         5367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_706002, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000006 DASTKM010000000
VERSION     DASTKM010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706002"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFQ10_00110"
     CDS             <1..203
                     /locus_tag="VFQ10_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963768.1"
                     /translation="GARVEKLATDEGTTCEGCGCARLRCGEEALRTHHHGLMVYLWCA
                     NEVHPRGAYQYFTIPAGENIGT"
     gene            380..1969
                     /locus_tag="VFQ10_00115"
     CDS             380..1969
                     /locus_tag="VFQ10_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET9963769.1"
                     /translation="MHEIESLIFLLAAAALLAQLARVLKVPYPILLVLGGLVMGFVPG
                     LPNIEIPPEVIFVAFLPPLLNSAAFSSSPLDLRAQLRPIVLLAVGLVLATMGAVAVIA
                     HALIGLPWAAAFVLGAILAPTDPVAAEAIFGRLGVPERVGTVVSGESLVNDGTGLVAF
                     RVAVAAVVSGAFSVWVAGLSFLLIGGGGMLVGLALARIILPLWARLREPTVVITFSVL
                     IPYAVYILTEEVLHVSGILAVVAYGLYQGWNSPRLFPNASTRLQTIAFWGVLVFLLEA
                     LLFVLLGQQLPSILADIEEYPVWVVLLYAALVYAVVVVVRFAWFFTTPYFHPVFDRLL
                     HSRYLRAPWQERLVMSWSGMRGAVSLAAALALPLSTDAGDPFPGRDLIVFLTFSVILA
                     TLVLQGLTLGPLIAALRLRGEGEADKLAELRARLDAARVALDKLERLGDDGRVSPESQ
                     ERMREYYEGNIQRYTAGLEAGGTTEEYAESSAAWRDWRRELLAAEREVVLSLRDEGKI
                     SPLVMRRIERDLDLQEARLGS"
     gene            2255..2393
                     /locus_tag="VFQ10_00120"
     tRNA            2255..2393
                     /locus_tag="VFQ10_00120"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2354..2356,aa:Phe,seq:gaa)
     gene            2603..3421
                     /locus_tag="VFQ10_00125"
     CDS             2603..3421
                     /locus_tag="VFQ10_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963770.1"
                     /translation="MLAAEPRADVHHGAPRCLLELFDTAAGSLADRSEFEAEVESWGI
                     EVRGLSREDLAALIEGSAREILTTEDRRLHREEGDFVRWGGDGGRRILALYGRLYFSL
                     LARLRWGCIDVEALIEHRARSLRGAGRCEPPSRVLLWCPRTQSARRPVVEEIIGRVPK
                     LTGAQLERLEDELRRERQRRASSAGDGGQDASHIQEGTVPPVTEVLEYRPYEDGYLQL
                     ELRRYIRRDGSARERGPYWYFQFHEGGKRRKLYLGKTSDPESTLGAKRSKPLGE"
     gene            4277..5143
                     /locus_tag="VFQ10_00130"
     CDS             4277..5143
                     /locus_tag="VFQ10_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9963771.1"
                     /translation="MGADDSTRGIAAEYWEELRKFRATHQPKHVTVKGVNWDYIASGE
                     GGEGLLLLPGGAMVGEAGFTRIPAFEDRYRVIAPDYPYVSTAALLLDGLAGVLDAEGV
                     REAHVLGPSYGGLVAQCFVRRHPERVRSLILTNTVVPPRLLLWPARIFLTLLPFVPLG
                     WLQALRERTLARAFSGVPGVPPEDQAFWRVYQHALVSRLSKEDLRGMGRLGIDLVESY
                     RFTPDELGSWAGRVLILESDEDIVTPEQRAELRRRYPQARVHTFHGAGHTPWMSHKEE
                     YLSVINEFLDQE"
     gene            5155..>5367
                     /locus_tag="VFQ10_00135"
     CDS             5155..>5367
                     /locus_tag="VFQ10_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9963772.1"
                     /translation="MTYTAWFDEIGKEDIALAGGKGANLGELSRAGLPVPPGYVVTTA
                     AYDTFVEVSGIKGEVVALASVPQAEDP"
BASE COUNT          965 a         1598 c         1767 g         1037 t
ORIGIN      
        1 agggcgcccg cgtcgagaag ctggccacag acgagggaac gacctgcgaa ggctgcggct
       61 gcgctcggtt gcgctgcgga gaggaggcac tccgcaccca ccatcatggg ctgatggtct
      121 acctatggtg cgccaacgaa gtccaccctc ggggggccta ccaatacttc acgatccccg
      181 ccggggagaa cataggcacc tagaggcgag tcttatgcac ccgagggcct gccagggatt
      241 ttctcggaac ctggtgagtt tcgctccact catcggttct tcctgttgga gatgttgaac
      301 gtattagact agtggcatga ggcggtcctt ggcatgctct tatgggcaac gcgagtagtc
      361 tcggacggtg ggcgcttagg tgcacgagat cgagtcgctc atcttcctgc tcgcagccgc
      421 agcactgctc gcccaactcg cccgcgtgct gaaggtgccc tacccgatct tgctggtcct
      481 tgggggcctc gtgatgggct tcgtgccagg cttgcccaac atcgagatcc cccctgaggt
      541 gatcttcgtc gcctttctcc cgcctctgct gaactcggcg gccttctcct cttcgccgct
      601 cgatctgcgc gcccaactca ggccgatcgt gctgctggcc gtgggcctcg tgctcgccac
      661 gatgggagcc gtcgctgtga tcgcgcacgc cctgatcggt ctcccgtggg cggcggcctt
      721 cgtactcggg gccatcctcg cccccaccga tcctgtggcg gccgaggcga tcttcggcag
      781 gttgggcgtg ccagagcgcg ttggcacggt cgtgagcggt gagagcctcg tcaacgacgg
      841 cacaggcctc gtcgccttca gggtcgctgt cgccgcggtg gtcagcggcg cgttctcggt
      901 gtgggtggcg ggcctgagct tcttgcttat cggcgggggc ggaatgctcg tcggtctcgc
      961 cctcgcgcgc atcatcctgc cgctgtgggc ccggctgagg gagcccacgg tcgtgataac
     1021 cttctccgtc ctgatcccct acgccgtcta catcctcacg gaggaggtct tgcacgtctc
     1081 gggcatcctg gccgtggtcg cctacggtct ctaccagggg tggaactctc cgaggctctt
     1141 cccgaatgcc tcgacgaggt tgcagaccat cgcgttctgg ggcgtgctgg tcttcttgct
     1201 cgaggcgctg ctcttcgtcc tgctcggcca gcagctcccc tcgatcctag ctgacatcga
     1261 agagtaccct gtgtgggttg ttctcctcta cgccgctctg gtctacgcgg tggtcgttgt
     1321 ggtgcgtttc gcctggtttt tcacaacgcc ctactttcac ccggtcttcg acaggctcct
     1381 gcacagcagg tacctgagag ccccctggca ggagcgcctg gtgatgagct ggagcggcat
     1441 gcgcggggcg gtctcgctgg ctgcagccct cgccttgccc ttgagtaccg acgccggcga
     1501 tccgtttccc gggcgcgacc tgatcgtgtt cctcaccttc tcagtgatcc tcgctaccct
     1561 cgtcctgcag ggactcacct taggtccgct gatagcggcg ctgcgcctga ggggcgaagg
     1621 cgaggctgat aagctcgcgg agctccgggc ccgcctcgac gccgcccgcg tggccctcga
     1681 taagctggag cgcctcggcg acgacgggcg agtttcaccg gagagtcagg agaggatgcg
     1741 tgagtactac gaggggaaca tccaacgcta cactgcgggc ctcgaagctg gcggcaccac
     1801 cgaggaatat gcggaaagct ctgcggcctg gcgcgactgg cggcgcgagc tgctcgcggc
     1861 tgagcgagaa gtagttttgt ccttgcgcga tgaaggcaaa atcagcccgc tagtgatgcg
     1921 tcgcatcgag agggatcttg atcttcagga ggcgcgcctc gggagctagt tgtcccacgg
     1981 atggatcggg gctgcgcagt agcataattg ttcacccaag tgcctagagg aggtgtactc
     2041 ggaagtcagc gaccctcgcg gggcttggtc aggcgcagaa gccctgcctc ggtgtggcgg
     2101 aacccgcact ccgggtagaa cccgcgcgag tgcggctcga agactacttg gcggagagcg
     2161 ggagtaacta atagactagt gaggcgagca gacttgcgcg ttctgcttcc ggtgctctga
     2221 agagtgagga ggccatgtca acagtagcgc aacagtaggg acagaaccag ctggtatcca
     2281 acggtactca gtggacataa atccgcttag gtatgcgagt tttccgcata cctatgggct
     2341 cttgcatatg actgaaaatc atggtgtccc cggttcgaat ccgggtcccg ccacttgaga
     2401 aagtcctgca aattaaggaa aaatatagaa attcgacgga gttgccgaag ccccgtttct
     2461 gaagcgtgtc aacagccggc tcaagaagga gtcctttcta ctgcggctat ggtagcgact
     2521 tgcgcgcctt ctatcgcgag tgtgtaggtg gcgagggtca tcctgatatg ggagtgacct
     2581 ggatcggcga aactagggcg gcgtgctggc cgccgaacct agggcggacg tccaccacgg
     2641 tgcgccgcgc tgcctgcttg agctctttga cacggcggct ggaagcctcg ccgaccggtc
     2701 agagttcgag gcagaggtcg agagttgggg tatcgaggtc cgtgggctct cccgcgaaga
     2761 ccttgccgcg ctgatcgagg gctctgcccg cgagatcctt accaccgagg atcgcaggct
     2821 gcaccgggaa gagggcgatt tcgtgcggtg gggcggagac gggggccgga ggatcctagc
     2881 cctctacggc cggctgtact tctcgctgct cgcccgcttg aggtgggggt gcatcgacgt
     2941 agaagccttg atcgagcatc gtgccaggag tttgaggggg gcgggtcgct gcgagccacc
     3001 ctcgcgtgta ttattatggt gtccgcgtac acaatcagca aggaggccgg tggtggagga
     3061 gatcatcggg cgggtgccca aactgaccgg agcacagctc gagcgcctgg aggatgagct
     3121 ccggcgcgag cgccagcgca gggcttcatc cgccggcgac gggggacagg atgcctccca
     3181 tatccaggag ggtaccgtgc ctcccgtcac ggaagtcctg gagtaccgcc cctacgagga
     3241 cggctatctg cagctggagc ttcgccgcta catacgtcgc gacggctcgg ctcgcgagcg
     3301 cggaccctac tggtacttcc agttccacga gggtggcaag cgtaggaagc tctacctggg
     3361 caagaccagc gacccagaga gcaccttggg tgccaagcgg tcaaagccct tgggggagta
     3421 ggtcgcttcc cctggtctgg ctctgcacgc aaggacgaga agcctcagct cgagacgttt
     3481 gggacctcgg aaggatgatg gacgccatac gggcgcaaaa tcgtcatcaa ccgaagctta
     3541 ccgcaaccgg gctggtacaa agcagaacgt agcggacagc aatcgttgcc agagagagca
     3601 ataaccgcat aaataagcca aattgctgga cgcatcagga caggctcgca tgtacggcag
     3661 caaactcgta atgagactct aggtcagcgt ttcgagtccg ctcgtcggct ctcaatactc
     3721 cctgcgctag cgctaagaca ccgaggcgga aaggcctcga tataccacct tcgctgaaga
     3781 aggcgaactt aagcacgatc tctaatccgc gcgcaaccgc aaaatcaggg gaagtcaggt
     3841 tctctgcctt gcttaccgag gacagcgttg agcgcctagt attgactaca gcatgcggat
     3901 tgctcgcgtc gaatcccgag tactcacata ttcacctgag caagcagaag atagctgttc
     3961 tcggaggatt gagctgcgcc cgaggaggga agctccccgg tggcggcgcg gggacggcgt
     4021 cttggccgcc actcccgcct cggcatagga tcggggaggc ggctcggtgc acgatggtca
     4081 cgtcgctgcc gaggttcggg gaagttcgag agggcatggg agcggttcat aatcgccgag
     4141 cgcgaggcgc tccccaggtt ttcgtgaacg caacctcagc ccgagatata ttaatagtcg
     4201 tagcagttta gtagttctca agatagtagg taatcaactt ggctatgcgg ctagggcatg
     4261 gagtaggatc ctggtgatgg gagccgacga ttctactagg ggcatcgcag ccgagtattg
     4321 ggaggagcta cgaaagttcc gggccacaca ccaaccaaag cacgtcaccg tcaaaggggt
     4381 gaactgggac tacatcgcca gcggcgaggg tggcgagggg ctcctgctct tgccgggtgg
     4441 ggcgatggtc ggggaggctg gcttcacgcg catcccggcc ttcgaggatc gctatcgggt
     4501 catcgcgccg gactatcctt acgtgtctac cgcggcgctt ctcctcgacg ggctggcggg
     4561 cgtcctggac gccgagggag tgcgcgaggc gcacgtgctc gggccttctt atggcgggct
     4621 cgtggcgcag tgcttcgtgc gacgccatcc cgagagggtg cgatcgctga tactgacgaa
     4681 caccgtagtg ccgccgcgac tattgctgtg gccggcgagg atatttctga cgttattgcc
     4741 tttcgtccca ctcggatggc tgcaggcgct tagggagcgc acgctcgcgc gggcattctc
     4801 tggtgtgccc ggcgtgccac cggaggacca ggcattctgg cgcgtctacc agcacgcgct
     4861 cgtgtcgcgc ctctcgaaag aggaccttcg cggcatgggc cgactcggga tcgacctcgt
     4921 ggagagctac cggttcacgc cggacgaact cggctcctgg gcaggacggg tcctgatcct
     4981 cgagtcggac gaggacatcg taacgcccga gcagcgcgcc gagctcaggc ggcgctaccc
     5041 gcaggcccgg gtgcatacgt tccacggggc cggacacact ccctggatga gccacaagga
     5101 agagtatctt tccgtcatca acgaattcct cgaccaagag taagggttta ggacatgact
     5161 tacacggcat ggttcgatga gataggcaag gaagatatcg cgctagcggg cggcaagggc
     5221 gccaatctgg gcgagttgag ccgcgcgggg cttcccgtgc cacccggcta cgtggtaacc
     5281 accgctgcat acgacacctt cgtcgaggtg agcggcatca agggtgaggt cgtagccctc
     5341 gcctcggtgc cccaggcaga ggacccg
//
LOCUS       DASTKM010000007         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1410721, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000007 DASTKM010000000
VERSION     DASTKM010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410721"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="VFQ10_00140"
     CDS             <1..1979
                     /locus_tag="VFQ10_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET9963773.1"
                     /translation="VKDLRLGVDVGGTFTDLVALGDGGILTAKVPSTPSDQSEGVMNA
                     IEASQVEAGALVALAHGMTVATNALLERRGARTAIVTTAGFRDVLEIARQNRPSLYDL
                     AQERPPVLVPRGLRFTVEERMGPDGELAPLDSASLEAAIAAVEEAQVEAVAVCLLFAF
                     AHPEHERRVGEALREALPKVYVSLSSQVLPEFREYERFSTTTADAYLAPKLAAYLGRL
                     AERVDESGGPPPLIMQSSGGVTSVEDAISDAAGCVLSGPAGGVVGAAYIGSLGGYKDL
                     LTFDMGGTSTDVAPILAGEAQTTTETVVAGVPIKLPMVDVHTVSAGGGSIAWADAGGA
                     LRVGPHSAGAEPGPAAYDMGGDEPAVTDANFFLGYLADGTELGGEVVLRRDLSEKALA
                     ALGEKLSLAAEDVALGTVRVANAEMTRALRIISVERGLDPREFALLAFGGAGGMHACA
                     LAEELGMQTVLVPRAGGVLSALGLAISDIRRDYVSPYLSPLDEVNAEEFERRFAQMEG
                     KAEGDLDAPEHTRRADLRYGGQSFELTVEADYLEKLEHRFHAAHEQRYGYRMEDEAVE
                     LVNVRLISTVPVEKPELTEPEARGDAGAGEREANFDGEWMEVPVLDREEMGEGSEVEG
                     PAIVEFRESTCVVRPGWQGVVDGVGTLVLEKDDG"
     gene            1972..>3032
                     /locus_tag="VFQ10_00145"
     CDS             1972..>3032
                     /locus_tag="VFQ10_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET9963774.1"
                     /translation="MGRLDPVTLSVLASALAGIAEEMGAVLIRGSYSSNIKERRDCST
                     ALFDSEGRMVAQAEHIPVHLGAMPEAVAAIMSRDPEPGDVFAINDPYSGGTHLPDVTL
                     VSPVAYEGEIIGYAVTRAHHSDVGGMRPGSMPSDSREIYQEGLIIPPVRLVRGGDYVG
                     DVLELLLANVRTPDLRRGDLRAQIAANNIAEERVGELIERRGKDTVLTAFEEVISYAE
                     KRTREAIGDLPDGEHSAEDCMEGDGITEEDITIRTTVTVDGESMRIDFSGTADAVSGN
                     INCPLPVTRSASYFALRVLLPKDILANAGTYAPLTIEAPRGSLVNAAYPAAVVAGNVE
                     TSNRIADTVLAALAGFAPE"
BASE COUNT          525 a         1022 c         1002 g          483 t
ORIGIN      
        1 tcgtgaagga tctccgcctc ggagtggacg tcggcggtac tttcaccgac ctcgtggcgc
       61 tcggagacgg agggatcctg accgccaagg tcccctccac ccccagcgac cagtcagaag
      121 gcgtgatgaa cgccatcgaa gcctctcagg tcgaggcagg cgccctcgtc gccctggccc
      181 acggcatgac ggtcgcgacg aacgccctcc tcgaacgcag gggcgcccgc acggccatcg
      241 ttacgaccgc cggcttcagg gacgtgctgg agatagcccg ccagaatcgg ccctcgctct
      301 acgacctggc gcaggagcgc ccgcccgtcc tcgtcccgcg tgggctgcgc ttcaccgtag
      361 aggaacggat ggggcctgac ggagagctgg cgccgctcga tagtgcgagc ctggaagccg
      421 ccatcgccgc cgtggaagag gcgcaggtag aggcggtagc tgtgtgcctg ctcttcgcct
      481 tcgcgcatcc agaacacgaa cggcgcgtcg gcgaggcgct gcgggaagcg ctccccaaag
      541 tgtacgtctc ccttagcagc caggtcctgc ccgagttccg cgagtacgag cggttctcca
      601 cgacgaccgc cgacgcctac ctggccccga agctcgccgc atacctgggg aggctggcgg
      661 agcgggttga cgaatccggg ggcccacctc cgctcataat gcagtcctct ggcggagtaa
      721 ccagcgtgga agatgccatc tccgacgccg caggctgtgt cctctcgggc cctgcgggag
      781 gtgtagtcgg cgctgcctac atcggctctc tcggtggtta caaggatctc ctcaccttcg
      841 acatgggcgg tacgagcacg gacgtcgccc cgatcctggc tggcgaagct cagacgacca
      901 cagagacggt ggtcgcagga gtccccataa aactccccat ggtcgacgtt cataccgtca
      961 gcgcgggcgg cggctccata gcctgggccg acgccggcgg cgcactgcgc gtcggccccc
     1021 attctgcggg tgccgagcca ggacccgccg cctacgacat gggcggcgat gagcccgcgg
     1081 tgaccgacgc caacttcttc ctcggctacc tcgccgacgg cacggagctt ggcggtgagg
     1141 tggtgttgcg gcgcgatctc tcggagaagg ccctcgctgc gctcggggag aagctgagcc
     1201 tcgcggccga ggacgtcgcc ctgggtaccg tgcgtgtggc gaacgcggag atgacgcggg
     1261 cgctaaggat cattagcgtc gagcgcggcc ttgacccgcg ggagttcgcc ctgctcgcct
     1321 tcggcggggc aggcgggatg catgcctgcg ccctcgccga ggaactgggc atgcagaccg
     1381 tcctcgtccc gcgcgccggc ggcgtcctct ccgccctcgg cctcgccatc tcggacatcc
     1441 gccgtgacta cgtgagcccc tacctctctc ccctcgacga agtgaacgct gaagagttcg
     1501 agcgtaggtt cgcgcagatg gagggcaagg cagaaggaga cctcgacgct cctgaacaca
     1561 cgcgcagggc tgacctccgc tacgggggcc agtccttcga gctgaccgtg gaggctgact
     1621 acctagagaa gttggagcat cgcttccacg ccgcgcacga gcagcgctac ggctaccgca
     1681 tggaggatga ggcggtcgag ctcgtgaacg tgcgcctcat ctccacggtc cctgtcgaga
     1741 aacccgagct cactgagccc gaggcgaggg gcgacgccgg ggccggagag cgggaggcca
     1801 acttcgacgg ggagtggatg gaggtgcctg tgctggaccg tgaggagatg ggcgagggtt
     1861 ccgaggtaga gggtcctgcc atagtcgagt tcagggaatc cacgtgcgtt gtcaggccag
     1921 ggtggcaggg cgtggttgat ggtgtaggca cgctcgtgct ggagaaggac gatgggtagg
     1981 ttggatcctg taaccctctc ggttttggcg agcgctctgg ccggcatcgc cgaagagatg
     2041 ggggctgtcc tgatccgggg ctcctactct tcgaacatca aggagaggcg cgactgctcg
     2101 acggcgctct tcgactcaga gggccgcatg gtagcccagg ccgaacacat cccggtccac
     2161 ctcggcgcga tgccagaggc cgtcgcagcc ataatgagcc gcgatcccga gcctggcgat
     2221 gtcttcgcca taaacgatcc ctactccggc gggacccacc tgcctgacgt aacgctcgtc
     2281 tcccctgtcg cctacgaggg tgagataatc ggttacgcgg tcacgcgggc ccatcactca
     2341 gacgtcggag gcatgcgtcc gggctctatg ccaagcgact cgcgtgagat ctaccaggag
     2401 ggcctcatca tcccgccggt ccgtcttgtg cggggcggcg attacgtcgg cgacgtgctc
     2461 gaactcctgc tcgccaacgt ccgcaccccc gacctcaggc gcggcgacct gcgcgcccag
     2521 atcgccgcga acaacatcgc cgaagaacgc gttggagagc tgatagagcg tcgcggtaaa
     2581 gacaccgtcc tcaccgcttt cgaggaggtg atctcctacg ccgagaaacg cacccgcgag
     2641 gccattggtg acctccccga cggcgagcac tccgcagagg actgtatgga gggcgacggt
     2701 atcacggagg aggacatcac catacggacc accgtgaccg tcgacggcga gtcgatgagg
     2761 atagacttct caggcaccgc cgacgccgtc agcggcaaca taaattgccc tcttcccgtc
     2821 acccgctccg caagctattt cgccctgcgc gtactgctac ccaaggacat actcgccaac
     2881 gccggcacct acgccccgct caccatagag gcccccaggg ggagcctggt caacgcggcc
     2941 taccctgctg cggtcgtggc cgggaacgtc gagacctcca accgcatcgc cgacaccgtg
     3001 ctcgccgcgc tcgctggctt cgccccagag ag
//
LOCUS       DASTKM010000008        27589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_530059, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000008 DASTKM010000000
VERSION     DASTKM010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 27589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27589
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530059"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..826)
                     /locus_tag="VFQ10_00150"
     CDS             complement(296..826)
                     /locus_tag="VFQ10_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HET9963775.1"
                     /translation="MRRTFGPVTVECVLGDITSQDDVEAVVNAANARLEPGGGVAGAI
                     HRAAGPGLAEEARPLGPIQPGEAVITGAYGLPNRYVVHTLGPVYGQDRPEAELLEGCY
                     RNSLALAEEYSVESIAFPAISTGIFGYPVEEAAGVALGTMREEANGLEHVRLVRFVLH
                     SASDLQVHERILSELE"
     gene            complement(823..1971)
                     /gene="nagA"
                     /locus_tag="VFQ10_00155"
     CDS             complement(823..1971)
                     /gene="nagA"
                     /locus_tag="VFQ10_00155"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET9963776.1"
                     /translation="MRLVALRGQIVTDYEVWPDGTVLLGDGSIADVSPDDSLVAEADE
                     VHHHPESLILPGFVDLQVNGAFGVDVASESARLPELSEALLSTGTTSYLPTVISSPES
                     LYEDVLPAIGTSTGSGAEVLGVHLEGPFISVQKKGAHAAAHIVMPDAELLAHLLDLGP
                     VRMITLAPELEDADRLISLAVNRGVVVSAGHSDVAFEPAYDALDGHVAGVTHLFNAMS
                     AMHHREPGLPGAAFAHPRAVCSLISDGLHVHPEMVGLAFRMLGPDRLCLVTDAIAATG
                     MEDGEYRLATRTVYGDDGIPRLGSGSIAGSVLTMREAFMNILAFTGCTVPEAARMTST
                     SPARLVGEGRRKGRLVPGYDADVTVLTPDLSVESVWRGGKLAYSWGGG"
     gene            complement(2020..3399)
                     /locus_tag="VFQ10_00160"
     CDS             complement(2020..3399)
                     /locus_tag="VFQ10_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9963777.1"
                     /translation="MLYTAAEMRERRLSDGKTSRLAVFSWILYDFSNTIFSVTILSFY
                     FPLWVEEQVGSGGLFSGAGLVNSATAISALFVVLTAPVLGAVADLRQRRVPYLILLTI
                     IAVALTAALDVAGGVVVGLALFVAADLAYQSALVFYNALLPVVSAGRGAGRISGYGTA
                     VGYVGTILGLIVLTYFVENGETVHPLLGPLGGWLGTGDEVYSNAFVPTAVIYLVFSLP
                     AFFFVPDPAVRARRPVVLWATYRDVVRTVKNLRAYAGVGTFILATVLYTDAANTAIAN
                     MALYGRVVFEMDGTQVRNLLLFSTVFAVVGSAVFGLLTDRVGPRRSLVGVLVLWLVAI
                     VLSAAALGAWMLFLAGPIVGIALGGMWTVGRVMLVALSPPEKLGEFFGLFSLAGKVSA
                     IFGPALTAVLLFIFGGGAYGYRISIGSLAIIMALGLFFLLRVPDARPERNVDEFAPKT
                     GTPLDSPAP"
     gene            complement(3471..4787)
                     /locus_tag="VFQ10_00165"
     CDS             complement(3471..4787)
                     /locus_tag="VFQ10_00165"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET9963778.1"
                     /translation="MSGRADEVIRGVEGVLPGESATARAAHESLKGDRRGIRKLLPFL
                     GPAFIAAVAYVDPGNFATNISGGAQFGYLLLWVILVSNLMAMLIQSMSAKLGIATGKN
                     LPEVCRARFPRPVTFLLWVQAEVIAMATDLAEFIGAALALNLLFGIPLFPAGLLTAVG
                     AFGILELQRRGFRPLEAVITAMVGVIVVAFAFQMFYAEPEADRILAGLFTPGFAGTES
                     VLLAAGILGATVMPHVIYLHSALTQRRVVGKTEEEKRKIFRFELVDVVLAMAIAGTIN
                     ASMLIMAAALFHSAGLTGVGDIDEAFEQLRILEGNTPAILFGVALLASGLSSSSVGTL
                     SGQVVMQGFISRQIPLFLRRLITMMPALIILAMGLNPSRSLVISQVVLSFGIPFALVP
                     LLIFCRNRDVMGTLVNHRLTTTVATVVVFLIVSLNVFLLYQTFFSA"
     gene            4904..5590
                     /locus_tag="VFQ10_00170"
     CDS             4904..5590
                     /locus_tag="VFQ10_00170"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET9963779.1"
                     /translation="MNDLAENRSPSSSVGDYLKAVWELAVDSGSAASTKSVATRLSVS
                     PASVTNMFARLQEMGLVRYERYRGATLTERGRKEALRLVRRHRLIETFLLEHLGYDWQ
                     EVHDEAERLEHAVSDGFTGRLAELLGHPDHDPHGDPIPSAEGTLEVDDSFTLSQAVAG
                     QRLRISKVRDEDAAMLDYLGERNLVPGRWLSVREVRTLDGVVIVGDEEAEVHALGEPL
                     ARSIFVRNDS"
     gene            5681..6583
                     /locus_tag="VFQ10_00175"
     CDS             5681..6583
                     /locus_tag="VFQ10_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HET9963780.1"
                     /translation="MHQDGDAQRDASKAIQSRKLEKVVVEDPPPEPDYPRYRVEPGGR
                     ASLAAIDPDETEHYRKKKDVARELKKQRRRIQDLQERLYAENERGMLIVLQAMDTGGK
                     DGTIKHVFSGVNPQGCRVSSFKAPSPEEANHDFLWRYHKSAPAKGRIGIFNRSHYEDV
                     LVVRVKNIVPEEVWRERYELINNFERGLTANGITVLKFFLHISKDEQKERLQSRLDDP
                     DKRWKFSSNDIKERAYWDAYQQAFEDALDNCSTEHAPWYVVPANKKWYRNLAVARTIA
                     DTLEAMDPRYPPAEEGLEKVTIPD"
     gene            complement(6681..7841)
                     /locus_tag="VFQ10_00180"
     CDS             complement(6681..7841)
                     /locus_tag="VFQ10_00180"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9963781.1"
                     /translation="MDEKERLRRWRLVLGGDSEATIGAGLVGEDRGMDGVLAALYGGE
                     KDSKNVGHGGSLPNVTRWLGDIRTYFPSPVVRVMQKDALDRLGLQRMLLEPEMLEAVE
                     PDVHLVATLISLGRVIPEKTKDSARRVVRKVVEELERRLTRETEQAVRGALNQAARTS
                     RPRPADIDWERTIRANLKNYLPELGTIIPEKLVGHARRKGTAKLRDIVLCVDQSGSMA
                     TSVVYSGIFGAVLASIRAVNTRMVVYDTSVADLTEDLQDPVDLLFGTQLGGGNDTPRA
                     LAYCQSIITRPAETVMVLVTDCYEGGLSEAMLERTVRILDSGVTMVCLLALSDDGAPS
                     YDRRQAAAMASLGVPTFACTPDLFPGMMAAAIQKQDLDLWAARNEIVTTRGS"
     gene            complement(7834..10032)
                     /locus_tag="VFQ10_00185"
     CDS             complement(7834..10032)
                     /locus_tag="VFQ10_00185"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HET9963782.1"
                     /translation="MAIRVFGIRHHGPGSARSLLWALDELRPDAVLVEGPSDADAVLP
                     LMARPQMQPPVALLVYPPDAPQDAAYYPFARFSPEWNALRYALGSGVTARFVDLPRAL
                     DLEDEDGAEPEVREDPIGLLAEAAGYEDRELRWERQVEQRRDATDLFDAIIEAMAALR
                     EDYEPPPREARREAYMRREIHKTLQQGHENIAVVCGAWHAPALRLEDHAVKSDDALLK
                     GLKRRKTSATWIPWTTARLSYRSGYGAGIASPGYYEHLWSCPEGSVASRWLSLVARLL
                     RSEDLDAPPANVVEAARVADTLAAIRDLPAPGLDELREAALATLCGGETERLALIRDR
                     LEIGEKLGEVPAETPTVPLQRDLEGLQKRLRLKPSAEERHLDLDLREVSGRARSELLH
                     RLRLLGVGWGEPHAVSGKSGTFHEVWTLQWRPELSLALIEANVWGNTVEAASASRVRE
                     LAETADLPHLTALLDVAILARLPESAVGHLLGRVRDNAASADVRGLMDALPPLARVAR
                     YGDVRGTAAASVLPVIDVLFERALVSLPGACTSLDDDAAREMAESIAAGEECVNLLDR
                     SGMHEKWRTVLGPLAENESVNALLRGWCCRLLLEAGSLDEEELQRLAGLSLSPAAPAT
                     VAAAWVEGVLRGSGLVLLHQTGLWMALDGWLTGLDPETFTSLLPLVRRAFSDFEPAEK
                     RAMGERVRKLASGGEAGPAHGYDGTGDLDHRRAARTLPVLAQILGVERDG"
     gene            complement(10035..10535)
                     /locus_tag="VFQ10_00190"
     CDS             complement(10035..10535)
                     /locus_tag="VFQ10_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963783.1"
                     /translation="MIATANNRDKGVNELSSALTRRFNTVVLPVPETTEEEVEIVERR
                     VASLGRALELPAERPALEEIRRVVTVFRELRGGVTEDGRTKLKTPTGTLSTAEAISVL
                     TAGLASAAFYGDGAMKANDVAAGLTGAIVKDPVQDRVAWLEYLQTVVRERKDWKDLYR
                     ECREVV"
     gene            complement(10548..11126)
                     /locus_tag="VFQ10_00195"
     CDS             complement(10548..11126)
                     /locus_tag="VFQ10_00195"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9963784.1"
                     /translation="MTDGPSALLRQHAEQEYAEELAELAKADDRQKPPSWKLSPWAVK
                     TYLMGGKLPNGFTVSPKYVGNGRLMEMAVATLATDRALLLYGIPGTAKSWVSEHLSAA
                     VSGDSTLLIQGTAGTDEAAIRYGWNYARLLTEGPSEAALVESPMMNAMRSGRLCRIEE
                     LTRIAPEVQDTLITVLSEKALPVPELNTEVQA"
     gene            complement(11123..12406)
                     /locus_tag="VFQ10_00200"
     CDS             complement(11123..12406)
                     /locus_tag="VFQ10_00200"
                     /inference="COORDINATES: protein motif:HMM:NF039502.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HET9963785.1"
                     /translation="MDQRTSILLEALERLRLAGLRLPHALLPGAMNVVEELRPAVVAV
                     LGERGRWLARLNPEWAWAVGDEGDLRDVWDEGDLKERTSVLRRVRGRDPALGRAWLSE
                     TWRQEKADARSAMLEAFEENLSPEDEPFLESSLGDRSMKVRAVAADLLARLPGSAYAL
                     RYAERAEAIIAGYEPPSTGFLGRRRSGKLMVELPPAVDEGWKRELPGEASPPRKMGER
                     AWTVVRTLEVIPPVYWERRFGATPEELVAAARGEWEAVLLTGWRRAAVRHGQESWAWP
                     LWRRWHEAPETQHDLTSTLELLAPLLPHAELGGVLRSSGMSHELAYTLNALPGPWDEE
                     LSLLYLEKLRKHVPRTFARNRDVSDPWLGTPQPAAERIALSCLDHATVLQPDEVLPER
                     MFSDGQVLRYYWREKLEKFEETLELRRRLVKEIPL"
     gene            complement(12667..13998)
                     /locus_tag="VFQ10_00205"
     CDS             complement(12667..13998)
                     /locus_tag="VFQ10_00205"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HET9963786.1"
                     /translation="MNTGLTSEGVAALAPDAKVAAAGKKLGAPKFWQDLGRNQEALWG
                     ECRGSAVYQVRVDLSDLAVKCSCPSRKQPCKHVIGLLFLSLEATPSETSSPDWVTDWL
                     ARRAARTTGGKRSKGRDEAAAPDLVARERREKQRLSRISAGLDALGLWMEDLVRRGLA
                     AAGTQPPSFWEEQAKRMVDAQAPGVAARLRRLSGLPNSSPDWPVKLLDGLGRLALLSK
                     AFSRLDVLDDPLVESVRAEVGISLPQEEVLERGERVDDRWIVLGQRTEEEGRLTVRRT
                     WLLGEDTRRYALVLQFAAAGSPFAEGFVSGTKWRGELAFYPGAYPLRAVVRSRSGGGD
                     RCEGLPGHDTVEAFLDHTSKATALQPWLERLPAALRGVVPLLDGVSEGGRWLIRDRAG
                     LALPLAGGEHWTLLSMSGGRPVDLAAEWDGEGLIPLGVAAGGTYCILAEEG"
     gene            complement(14268..15224)
                     /locus_tag="VFQ10_00210"
     CDS             complement(14268..15224)
                     /locus_tag="VFQ10_00210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HET9963787.1"
                     /translation="MPEKVLVTREIPAAGLRALEPFDVRILHERPPEREELLDAVRGV
                     SGVLSTATEKVDGELMDAAGKDLRVVANMAVGYDNVDVEAATERGLVVTNTPGVLDET
                     TADVAFMLLLAAARRLGEGERLLRAGRWEWWGPKQLMGRDVWGKVLGIVGFGRIGQAV
                     ARRAKGFGMEILYHNRSRKEDAEQELGARYLDLDELLESADFVSVHTPLSGETHHLIG
                     EAELGRMKPEAIFVNTSRGPVVDEAALADALAEGRIFAAGLDVYEEEPHVHPKLLELQ
                     NVVLAPHIGSASVETRDRMAALAAENIVAVLSGEGPKTPVGR"
     gene            complement(15246..18191)
                     /locus_tag="VFQ10_00215"
     CDS             complement(15246..18191)
                     /locus_tag="VFQ10_00215"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET9963788.1"
                     /translation="MANDPFGARTTLKTGSGDVELYSLKTLDKNVDGDVFSLPFSIRV
                     MLESLLRNAGGKFVSKEDVEALAEWPESVGGELAYLPARVVMQDFTGVPAVVDLAAMR
                     SAMANVGGDPSRINPLVPTDLVIDHSVQVDAFGSPAALQFNAEREFERNRERYEFLKW
                     GQQAFDNFSVVPPATGIIHQVNLEYLAKGVIVKDGVAMPDTLVGTDSHTTMINGLGVL
                     GWGVGGIEAEAVTLGQPYYMLVPEVIGFKLTGALKEGVTATDLVLTVTQMLRSHGVVG
                     KFVEFYGEGLSKLSLPDRATIANMSPEFGATCTFFPVDTETLRYLRGTGRDEELVELV
                     EAYSKEQGLWRTDETPEPRFSETLDLDLESVEASLAGPRRPQDRVALDDMQPSFRQAL
                     AEMVDPDHPLLGNGYEDAYNHADEESFPASDTPNPDIPVARGGTNEAEGKEGGAISTG
                     ESHTADDTESFPASDTPAGASGVSNEDGGDPTAGGEPENGKPEAEPTGAITVTVDGEE
                     LYLRHGSAVIAAITSCTNTSNPSVMMGAGLLARKAVEKGLSVAPHVKTSLAPGSKVVT
                     EYLQTSGLLDPLEKLKFDVVGYGCTTCIGNSGPLAEEISAAVEENDLVVAAILSGNRN
                     FEGRINPDVRANYLASPPLVVAYALAGTVDIDLSKDPIGEDDSGTPVYLQDIWPSQEE
                     VAREIENALDPEIYREQYADVYTGNEQWNDVEVPSGDLYEWDPDSTYIQEPSFFKDMG
                     PEPEDLEDIEGACVLVKVGDSVTTDHISPAGAIPSKMPAGQYLIQKGVHPRDFNSYGS
                     RRGNHEVMVRGTFGNIRLRNQLVDKEGGYTVHLPDGEETTIYEASLKYEVENVPLVVL
                     AGKEYGSGSSRDWAAKGSFLLGVRAVIAESFERIHRSNLIGMGVLPLQYAEGESAESL
                     GLSGRESFDISGLAGLEPGKQLTVKVTGDEGEAKEFKVKARVDSPVEVEYLRNGGILQ
                     TVLRQMLEE"
     gene            complement(18287..19903)
                     /locus_tag="VFQ10_00220"
     CDS             complement(18287..19903)
                     /locus_tag="VFQ10_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET9963789.1"
                     /translation="MNPSSSDRIRYLEQGGAALLGGAVAVLVGILYIGTLAPTVLPYG
                     APDTLDSPMLQTAVPVLGVGHPTGYPTYMMLTHFFTYLPFGDPAYRVNLASAVYGVAA
                     VLLVYLAGLRLARRAVAAAAGALAFGLSGAFWSQAVIAEVYTFEALLVALVILFLFLW
                     RDRREGRYLLLSAFLVGLSLTHHLTSVLLVPATLAFVFFTDRRVFSRKGLILKGVGLF
                     LLGLLPLLYLPIRALMHAPLNEADPSTPWRFLLLVTGGSFLAESSEKGRHCSPSSLTL
                     ADTSTKLGLFGDHLLGQIPLVLMLVGVLAAVYLLFADRATAMLLGTLFLGCLVQAAVY
                     LQLAIEDFYVFLIPAILTFGLCISAGLGALLRLVESLTIGSTARGALLVILSTLMLLV
                     PLLGVPGSYAAHDRSEDYGGRRMIGAVVSDVEKDATVLHHRSPLWYMVLVEGRRRDLT
                     LMDPFCTSWDRHTDVVWPDQISAAEAADRYGTDDTTGVESAREAAQTGPVYLLANGRA
                     RMDSFREAGFDVVPVERDGFLYELVPRSRG"
     gene            19962..20501
                     /locus_tag="VFQ10_00225"
     CDS             19962..20501
                     /locus_tag="VFQ10_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963790.1"
                     /translation="MAKRGYTRDTIRGGASEWDVSRPVSSYARTLAMLLVHPVRFFEL
                     LPRIPDIRAPALFLIFSGLPTAILWLPFWGLPPALVAVVLPLPLSFLLAGLYHLGVLG
                     GRHGYMVTWRVLAYPLGFYLPFAAVPMLGPLGAAYSGLVIIPLGLAEVHEVGRLRAVL
                     FCAAIGVVFGAAYYLLSVA"
     gene            complement(20520..21002)
                     /locus_tag="VFQ10_00230"
     CDS             complement(20520..21002)
                     /locus_tag="VFQ10_00230"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9963791.1"
                     /translation="MGSNYKVREAKVEDAAEICSLARELAETVGDSPSAEEAIRARLE
                     ELLDEPRAQVLVAENEAGIVGGVSFWIKPDLAHGDTVVEVPMLVVAEDHRRAGVGKLL
                     MEEVRNVASHNDASQIELAATRANVTAREFYRSLGFVETDVVSLEFVGSQEDPPDPEE
                     "
     gene            21103..22269
                     /locus_tag="VFQ10_00235"
     CDS             21103..22269
                     /locus_tag="VFQ10_00235"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET9963792.1"
                     /translation="MGSRAVVIVLDGLGAGEAPDAADFGDEGANTLANTARSTGGLRA
                     PNLQAVGLGNIEEIEGIAHSASPAASHGLMVELSAAKATLAGHWEMMGLVLEDPLPTY
                     PEGFPEDVLLRFENETGRGVIGNRPASGTTIIEELGPEQEKTGAWILYTSADSVFQIA
                     AHTGVIPLDELYEASKKAHKMLISEGEIMVERVIARPFHGRAGAYVRENENRHDYGIT
                     PPSETYLDRIKDAGHETVAVGKIKDIFDGRGITEHLPAPPDDAAKVDAILDALGHVES
                     GLIFANLVDFDASYGHRRDPEGMAANIQLFDERLPELLDALTAEDLMIITADHGNDPT
                     FRGTDHTRERVPLLVMGGGDTRDLGIRESFSDVGASVAAWLGVEKGGLPGRSFV"
     gene            22336..23610
                     /locus_tag="VFQ10_00240"
     CDS             22336..23610
                     /locus_tag="VFQ10_00240"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET9963793.1"
                     /translation="MGSGNAILDAIEAKKFGGELSHGLIQDVVEGYATGKVPDYQMAS
                     LLMAIFIQGMSYGETLALTRVMAESGEIYSFPECVDKHSTGGVGDKVSLTALPIVAAC
                     GAPVAKLAGRGLGSSGGTIDKLESIPGFSCNLSEEQFRNQVEEVGLAIIEAGDLAPAD
                     KEIYALRDTTGTVDSLPLLGSSIVSKKVATGAGHLLYDVKHGSGAFMKTIEEARQLAE
                     LLVRLSEDLGIDASALITDMHNPLGSAVGNALEVRESVRLLKNEPVPDDLVSEVNHVA
                     THLLTQKGIPEAAAAVENALSSGAAYEKFRAFVKAQGGDADALEDLPVSAEVREVKSP
                     RGGFVARFGASGIGSAALVLGAGRKQKGDRIDPGSGIEVLVKPGDPLQQGQPVARLYG
                     EREAELARRLVVEALEISDEPVEPPPVILDSL"
     gene            23846..24559
                     /locus_tag="VFQ10_00245"
     CDS             23846..24559
                     /locus_tag="VFQ10_00245"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoD-type purine-nucleoside phosphorylase"
                     /protein_id="HET9963794.1"
                     /translation="MCPVHVRAEPGDFAESVLLPGDPLRAKYIAETFFEDAKLVTEER
                     GMLGYTGTYRGKPVSVQATGMGCPSASIVTEELIQLGVRNLLRVGTCGGYSRELRLGD
                     LIVATSAAPQDGTVSSITRGVPYAPAAHFDVVHAAHHAAESAGRRTFIGQVVSSDLFY
                     DPTEDPATLWGALGVLAVEMEAAAIFTIAAMRGVKAGCLLTVSDTIGAEIVRISDDEL
                     RNAVDNMMALALDTLNALN"
     gene            24623..25954
                     /locus_tag="VFQ10_00250"
     CDS             24623..25954
                     /locus_tag="VFQ10_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HET9963795.1"
                     /translation="MTILNESGIPDVLPPLHAVNDLAQRLPDVLIVIAGTQAEAHLVQ
                     CLPSAPSPNLPRSRRNQRVIFAVLDPDDPPQQGLLASRIVEIAAGLRGARLVLLVAGR
                     AAESLGVDAAFEARLVTRKLDVPAEVVNPDAPHEVPGILSTDLEDRVLAALVNLCPKR
                     KTSDLVETASGAKRGGLFGSFLRRGTETSGSNGGRPVVLLGGVLSPGADQVLAAELSR
                     TGVEVVGSVPGSGIGKLPPLGEGTVVAVMDPNLSSAATVAERRGATVVKTLMPVGVDG
                     TARFVQDVAEEAGSRTSEVVRARSVWEDLGFLRNRIRGKRIFFTGDTGLEIPLARFLA
                     DAGAVVLEVGAPRLDRRFLAAELQVLGPDVDVVVSPDYRGQTNRIDESRPDVVISSPG
                     LQLPLVARGHLCRSPRDFIAAGLYGYGGSRRILELLVRTMERAETLDSLNL"
     gene            25951..27381
                     /locus_tag="VFQ10_00255"
     CDS             25951..27381
                     /locus_tag="VFQ10_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HET9963796.1"
                     /translation="MKLLRGVYEGPQSYGILRVAASIGGVHTLLRALPGEAYFRTLYG
                     ARERTGAMAPVTLSPLREGPGDSMAPGNLARDLSGVVRRHPKAEIFIVARSEASLLSE
                     NAITNLPEAEATGRRPKLVTFPWEGPGMREIEAAELALEDLIRSLMHHRPERSPYPTV
                     NLFGPPIFGPGAAAEYAEAERLLDMIGVEVNARVPLGAHAGDLDRLPRGWANVLLYRE
                     VGESATLYLQDVFGMPRVTTPMIGAAGTGAVLRSVGELCQLDPKSVQRAIWAELARTA
                     KLPWYARLEPPETFQDRRVAIFGDFTYPLGLGYALAREVGLEIIACGTYLTHLERDFL
                     FHAHTFTEGAFVEDDPDQVASRIEAAKPDLIIGTHLEEDVADSLGVPFLPLCPPVTDR
                     PFVGRPLMGYAGSSVLADALDNVLREIGVEPAGEASIQWTDGALQEVSEVPLFLRGRA
                     RRLAEERARRQGSAEVTREIFLESRS"
     gene            27401..>27589
                     /locus_tag="VFQ10_00260"
     CDS             27401..>27589
                     /locus_tag="VFQ10_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963797.1"
                     /translation="MGIRDFIHCLDEHQNRLNRIQSLSNREREETLREAETMVGMADA
                     AIKELESVLGNRRVNLPEE"
BASE COUNT         5091 a         8895 c         8847 g         4756 t
ORIGIN      
        1 cccttcagac cttcggcggc tacggcttcg acgccgagta cgatgtagag cgcaagttcc
       61 gcgagacgcg tctctaccag gtcgccccga tctcgacgaa cctcatcctc tcctacatcg
      121 ccgagcacgt gctcggcctc cccaggtcct tctgagagcg ggataagacc cttccttatc
      181 cggcgtcggt cagccagctg ctgatgtgct gacggctcgt cgtgcgtaag gctcaaagcg
      241 tcgtgcacgc tgctgtagag acactttagg caggttggat ctttccgcaa gacgcctact
      301 cgagttcgga gagtatcctc tcgtggactt ggaggtcgga ggcgctgtgg aggacgaacc
      361 tgaccaggcg cacgtgctcc aaaccgttgg cctcctcccg catcgtcccg agcgccaccc
      421 ccgccgcctc ctcgaccggg tagccgaaga tcccggtcga gatggcgggg aaggcgatgg
      481 actccacgct gtactcctcg gcgagtgcga gtgagttgcg gtagcagccc tccaggagtt
      541 ccgcctcggg acgatcctgg ccatagactg gtcccaaagt gtggacgacg taacggttcg
      601 gaagcccgta cgcaccggtg atgaccgcct cgccgggctg tatggggccg agcggcctgg
      661 cttcctcggc gagtccgggt ccggcggcgc ggtggatggc ccctgcaacc cctccgccgg
      721 gttcgagtcg cgcgttcgcc gcgttgacca cggcctccac gtcgtcctgg gaggttatgt
      781 ctcccaggac gcactcgacc gtaacgggcc cgaacgtacg tctcatccgc cgccccagga
      841 gtaggccaac ttgccgcccc gccacaccga ttccacggag aggtctggcg tcaggaccgt
      901 cacgtcggcg tcgtagccgg ggacgaggcg gcccttgcgg cggccttcgc cgacgagcct
      961 cgccggggag gtagaggtca tgcgcgcggc ctcggggacg gtgcagccgg tgaaggcaag
     1021 gatgttcata aacgcctctc tcattgtaag cacgctccct gcgatggacc cgctccctag
     1081 cctcgggatg ccgtcatcgc cgtacaccgt acgggtagcc agacggtact caccgtcctc
     1141 catgcccgtt gcggcgatgg cgtcggtgac gaggcagagg cggtcaggac cgagcatacg
     1201 aaaggcgagc ccgaccatct ccggatggac gtgcagaccg tcggagatca gactgcagac
     1261 ggccctcggg tgggcgaagg cggctccagg cagccctggc tcgcggtggt gcatcgcgct
     1321 catcgcgttg aaaaggtgcg tgacgccggc gacatgccca tccagagcgt cgtaggcggg
     1381 ctcgaaagcg acgtcggagt ggccggccga gacaaccacc ccacggttca cggcgaggga
     1441 gatcagcctg tctgcgtcct ccagctcggg ggcgagcgtg atcatgcgca caggcccgag
     1501 gtcgaggaga tgggcgagca actccgcatc aggcattacg atgtgggcag cagcgtgcgc
     1561 acccttcttc tgtacgctta taaagggacc ttcgaggtgg acgccaagca cctcggcacc
     1621 tgacccggtt gaagtcccga tggccggcag gacatcctcg tagaggctct cgggcgaaga
     1681 gatgaccgtg ggcaggtatg aggtggtccc cgtcgagagc agggcttcgg agagttcggg
     1741 caggcgggcg ctctccgagg caacgtcgac accgaaggcc ccgttaacct gcaagtcgac
     1801 gaagccaggc aggatcaggg attcagggtg atggtggacc tcatccgctt cggcgacgag
     1861 agagtcatca gggctaacgt ccgcgatgga cccatcaccg agaaggaccg tgccatcggg
     1921 ccacacctcg tagtccgtga cgatctggcc ccgcagcgcg acgagcctca tgttgcggtc
     1981 ctccgactat ccttccccct catggcgtac atcttccgct cacggggccg gcgaatcaag
     2041 gggggtgccc gtcttcgggg cgaactcgtc gacgttgcgt tcagggcggg cgtcggggac
     2101 gcggaggagg aagaagagcc ccaaggccat tatgatggcg agcgagccta tggagatcct
     2161 gtagccgtag gcgcctcccc cgaagatgaa gagcagcacg gcagtgagcg ctggcccgaa
     2221 gatcgcggag accttcccgg ccagggagaa gagcccgaag aattccccta gcttctcggg
     2281 cggcgagagg gcgacgagca tcacccgccc caccgtccac atccctccta gcgcgatccc
     2341 cacgatcggc cctgcgagaa agagcatcca agctcccagg gcagcggcag agagcacgat
     2401 cgcgacgagc cacagaacga gtacgccgac gagggacctc ctgggcccta ccctgtctgt
     2461 caggagaccg aagacggccg agccgaccac ggcgaagacc gtggagaaga gaagcaggtt
     2521 cctgacctgc gtgccgtcca tctcgaagac cacccgaccg tagagcgcca tgtttgcgat
     2581 ggcggtattc gccgcgtcgg tgtagaggac cgtagccagg atgaacgtcc ccacccccgc
     2641 gtaagcgcgc aggttcttca cggtgcggac tacgtcgcgg taggtcgccc agaggaccac
     2701 aggccgccgc gcccgcaccg cagggtcggg cacgaagaag aaagcaggca gcgagaagac
     2761 gaggtagata acggccgtcg gcacgaaggc gttggagtac acttcgtcac cggtgccgag
     2821 ccatcctcca agaggcccga gcagcgggtg caccgtctct ccgttctcga cgaagtacgt
     2881 cagcacaatg aggccgagga tggtaccgac atagcccact gcggtgccgt agccgctgat
     2941 cctgccggcc ccgcgccccg ccgagacgac gggcagcagc gcgttgtaga acacaagcgc
     3001 agactggtag gcaaggtcgg ccgccacgaa gagcgcgagc cccaccacga ccccgccggc
     3061 cacgtcaagc gcggcggtga gcgcgacagc gatgatggta agcaagatca ggtaaggtac
     3121 ccgccgttgc ctgaggtcgg ctacggcgcc gagaactggc gccgtgagca cgacgaaaag
     3181 cgcggagatg gccgtagccg agttgacgag cccggccccg gagaagagac ctccgctccc
     3241 tacctgctcc tcgacccaga gcgggaagta aaaggagagg atggtgactg agaagatggt
     3301 gttcgagaag tcgtagagga tccaggagaa aacagccagc cgcgaggtct tgccgtcgga
     3361 taacctcctc tctctcatct cggcggcagt atagagcacg ccgcactccg gagaagacta
     3421 cgctgtgcgt aagggttgag tcctacgcac agcgtgcaat cagccccgga tcatgcactg
     3481 aagaaagtct gatagagcag aaaaacgttc agcgacacga tgaggaagac tacgaccgtc
     3541 gcaacggtcg tggtgagccg gtggttgacg agcgtaccca taacgtccct gttgcggcag
     3601 aagatgagca acggaacgag cgcgaacggg atgccgaagg agagcaccac ctggcttatt
     3661 accagcgaac ggctggggtt gaggcccatg gccaagatga tcagggctgg catcatggtg
     3721 atgaggcggc gcaagaacag cggtatctgc cggcttatga acccctgcat gacgacctgc
     3781 ccgctaaggg taccaacaga agagctcgac agcccggagg cgagcagggc gaccccgaac
     3841 aggatggccg gggtgttgcc ctcgagaatg cgcaactgct cgaaagcctc gtctatgtcg
     3901 ccgacacctg tcagtccggc cgagtggaag agcgcggcgg ccatgatgag catgctcgcg
     3961 ttgatggtcc ctgcaatggc catggcgagg accacatcga ccagctcgaa acggaagatc
     4021 ttgcgctttt cctcttctgt cttgcctact accctgcgct gcgtgagggc cgagtgcaag
     4081 tagatgacgt gcggcattac cgtggcgccg aggattccgg ccgccagcag cacgctctcg
     4141 gtccctgcga acccaggcgt gaagagcccg gcgagtatcc tgtccgcctc gggctcggcg
     4201 tagaacatct ggaaggcgaa ggcgacgacg atgaccccga ccatagcagt gatgaccgcc
     4261 tccagcggcc tgaaacccct tcgttgcaac tcgagtatcc cgaacgcccc gacggccgtg
     4321 agtaggccgg cagggaagag cggtatcccg aagagcaggt tcaaagccag cgcagcccca
     4381 atgaactcag ccagatccgt cgccatcgct atgacctcgg cctggaccca cagcaagaac
     4441 gttactggcc gcggaaaacg cgcccggcaa acctcgggca gattcttgcc cgtcgcgatc
     4501 cctagcttgg ccgacatcga ctggatgagc atggccatga ggttggaaac caggataacc
     4561 cacaacagca gatacccgaa ctgcgcccca cccgaaatgt tcgtcgcgaa gttcccaggg
     4621 tccacgtacg caaccgcagc gataaacgca ggcccgagaa agggcaggag cttgcgaatc
     4681 cccctcctgt cacccttcag cgactcgtgc gcagcccgcg cagtcgcact ctccccaggc
     4741 aacactccct caacacctct aatcacctca tcagcccgtc cactcatgaa aacctctatc
     4801 gtcttgaatt tagccctggc taataatcct attgagctat aatgaatagt caaggaacct
     4861 tgctcttgcc aaatgctttg caccgtctaa cataggctat gagatgaacg acctcgcgga
     4921 gaatcgttcc ccttcttcct cggtggggga ttatctgaag gctgtttggg agctcgctgt
     4981 ggactcggga agcgctgcct cgaccaagag tgtggcgacg cggctctccg tctcgccggc
     5041 ctcggtgacc aacatgttcg cgaggttaca ggagatgggc ctcgtcaggt acgagcgcta
     5101 taggggagcg acgcttaccg agcgcggccg aaaagaggcc ttacggctgg tacggcgaca
     5161 ccgtctgatc gagactttcc tgttggagca cctcggttac gattggcaag aggtacacga
     5221 tgaggcagag cggcttgagc acgccgtttc ggacgggttc accgggcggc tggcggagtt
     5281 gttggggcat ccggatcacg acccgcatgg agaccccatc ccctcggcag aaggcaccct
     5341 ggaggtggac gactccttta cgctgagcca ggccgtcgcg ggccagcggc tccgcatctc
     5401 caaggtcaga gatgaggatg ccgcgatgct cgactacttg ggtgaacgca acctcgtccc
     5461 agggagatgg ttgagtgtca gggaggtaag gaccctggac ggtgttgtta tcgtcgggga
     5521 cgaggaggcg gaggtccacg ccctcggaga gccgctggcg cgttcgatct tcgtcaggaa
     5581 cgattcttag ccctctccta tcagaacgga gtcgggcagc tttcgcgctt cgtttctgag
     5641 gaaccgggta ggttacccgt acataagggg aaggagtatt ttgcatcagg acggcgacgc
     5701 gcagagggac gcgagcaaag ctattcagag taggaagctg gagaaagtcg tggtggaaga
     5761 cccgccaccc gaacccgatt acccacgcta ccgggtcgag ccgggtggca gggcatctct
     5821 agcggcgata gaccccgacg agaccgagca ttaccggaag aaaaaagacg tcgccaggga
     5881 gttgaagaag cagcgcagac gaatacagga cctccaggag cgcctctacg cggagaacga
     5941 gcggggaatg ctgatcgtgc tacaggccat ggatacggga ggtaaagacg gcaccatcaa
     6001 gcacgtcttc agcggcgtca acccgcaggg atgcagggta tcgtccttca aggccccgag
     6061 tcccgaagag gccaaccacg atttcctctg gcggtatcac aagagcgccc cggcaaaggg
     6121 ccggatcggc atcttcaacc gctcccacta cgaggacgtg ttggtcgttc gcgtcaagaa
     6181 catagtcccc gaagaagtct ggagagagcg ctacgagctg atcaacaact tcgagcgcgg
     6241 cctcaccgcg aacggcataa ccgtcctcaa gttcttccta cacatctcca aagacgagca
     6301 gaaagagcgt ctccagtcgc gcctcgacga ccctgacaaa cgctggaagt tctcctcgaa
     6361 cgacataaag gaacgagcct actgggatgc ctaccaacag gccttcgagg acgcccttga
     6421 caactgctcg accgagcacg ccccctggta tgtggtgccg gccaacaaga agtggtaccg
     6481 caacctcgcc gtcgcccgca ccatcgcaga caccctggag gcgatggacc cgcggtatcc
     6541 tcctgccgag gaagggttgg agaaggtaac gatacctgac tagaggaagt ccatgaaccg
     6601 ggagtggcgg cacgacggct atgtcatcag cactgaggcc tggacctgga aactttcgta
     6661 cctggcagcc ggcgacctgt tcaactgccg cgagtcgtca cgatttcgtt gcgggcggcc
     6721 cagagatcta ggtcctgctt ctgtatcgcg gcggccatca tgccggggaa gaggtcaggg
     6781 gtgcaggcga acgtcgggac gcccaaagag gccatagcgg cggcctgccg gcggtcgtag
     6841 ctcggggctc cgtcgtcgct gagggcgagg aggcatacca tcgtaacgcc gctgtccaat
     6901 attcggaccg tcctctccag catggcctcc gagagacctc cctcgtagca gtcggtgacg
     6961 agcaccatga ccgtctcggc cggtcgggtg atgatgctct ggcagtacgc tagggcgcgc
     7021 ggggtgtcgt ttccgcctcc tagctgcgtc ccgaagagga ggtccacggg gtcctgcagg
     7081 tcttcggtga gatccgcaac ggaggtatcg tagacgacca tcctggtatt gacggcccgt
     7141 atggaggcga ggacggcccc gaagatcccg gagtacacca cactcgtcgc catagagccg
     7201 ctctggtcaa cacacaggac tatgtcgcgc aacttcgcgg tcccctttct gcgcgcgtgt
     7261 ccgactagct tttcggggat gatagttccg agttcgggca ggtagttttt caggttggcg
     7321 cggatggtgc gttcccagtc tatgtccgcc ggacgtgggc ggctcgtgcg ggcagcctgg
     7381 ttcagggcac cgcgcacggc ctgctcggtc tcccgcgtga ggcggcgctc caattcctcg
     7441 accacttttc gcaccacccg gcgggctgag tccttcgtct tctccgggat gacgcggccc
     7501 agggaaatga gtgtggcgac caggtgtacg tccggctcga cggcttcgag catctccggt
     7561 tcgaggagca tgcgctgcag gccgagccgg tccagtgcgt ccttctgcat cacgcgcacg
     7621 acgggcgagg ggaagtatgt gcggatgtcg ccgagccagc gggtgacgtt ggggagagag
     7681 ccgccgtgcc cgacgttctt actgtccttt tcgccgccgt agagcgcggc gagcacgccg
     7741 tccatgcccc ggtcctctcc cacgagcccc gcgcctatgg tcgcctccga gtcgccgccg
     7801 aggacaagcc gccagcggcg caggcgctcc ttttcatcca tcgcgctcca cccccagtat
     7861 ctgagcgagg acgggcagcg tcctggccgc ccgcctgtgg tccaggtcac cggtcccatc
     7921 gtacccgtgc gccgggccgg cttcccctcc actcgccaac ttcctgactc tttctcccat
     7981 cgcgcgtttc tcggcgggct cgaagtccga gaaggcgcgg cggactaagg gaaggagact
     8041 ggtaaaggtt tcggggtcga ggcctgtcaa ccagccgtcg agggccatcc agagtccggt
     8101 ctggtggagc agcaccagtc cactcccccg caatacaccc tcgacccatg cggcggccac
     8161 cgtggcgggg gcggccggcg agagcgagag cccggcgaga cgttgcagtt cttcctcatc
     8221 gagagatcca gcctcgagga gcaaccggca gcaccagccc ctgagtaggg cgttgacgct
     8281 ttcgttctcc gcgagcggac ccaggacggt acgccacttc tcatgcatcc ccgaccggtc
     8341 gaggaggttg acgcactctt cgccggcggc gatgctctcg gccatctccc tggctgcgtc
     8401 gtcgtccagg gaagtacacg cgccggggag ggagacgagc gcgcgctcga agaggacatc
     8461 tatcacgggg aggacactgg cggccgccgt gccgcgtacg tcgccgtagc gggcgacacg
     8521 ggcgagagga gggagggcat ccatcaggcc tcggacgtcg gcggaggcgg cgttgtcacg
     8581 cacccgaccg agcaggtgcc ctacggcgct ctccgggagc cgggcgagga tggcaacgtc
     8641 gaggagcgcg gtgaggtggg gaaggtccgc ggtttccgcg agctcgcgta cgcgcgatgc
     8701 tgaggcggcc tccacggtgt tgccccagac gttggcttcg atcagggcca aggaaagctc
     8761 gggacgccac tgtagggtcc agacctcgtg gaacgtgccg ctctttccgg agacggcgtg
     8821 cggctcgccc cagcctacac cgagcagacg cagacgatgg agaagctcgc tgcgggcgcg
     8881 cccgctaacc tcacgcaggt caaggtcgag gtgacgctcc tcggcagatg gtttcaagcg
     8941 caggcgcttt tgcagaccct ccagatcgcg ctggaggggg accgtggggg tttcagcggg
     9001 gacttcgcca agcttctcgc ctatctccag ccggtcccgg atcagggcta atctctccgt
     9061 ctctccgccg cacagcgtcg ccaaggcggc ctcgcgcagc tcgtcgaggc cgggagccgg
     9121 gagatctcgg atggcagcca gcgtgtccgc cactctggcc gcctcgacga cgttggctgg
     9181 aggggcatcg aggtcttcgg agcgcagcag acgagcgacg agggagagcc agcgggaggc
     9241 gacggacccc tcggggcagc tccagaggtg ctcgtagtag ccgggggagg ctatgccggc
     9301 gccgtagccg gagcggtacg agagacgagc tgtagtccag gggatccagg tggccgaagt
     9361 cttcctcctc ttcaaaccct tgaggagagc gtcgtcggac ttgacggcat ggtcttcgag
     9421 gcgcaacgcg ggcgcgtgcc aggccccgca cacgacggct atgttctcgt gcccttgctg
     9481 gagggtcttg tgtatctcac gccgcatgta cgcttcgcgg cgggcttcgc gcggcggggg
     9541 ctcgtaatct tcccgcaacg cggccatcgc ttcgatgatg gcgtcgaaga ggtcggtggc
     9601 gtcgcggcgc tgctcgacct ggcgctccca cctcagctcg cggtcctcgt atccggcggc
     9661 ttcggcgagg agaccgatcg ggtcctccct gacctcgggc tccgcaccgt cctcatcttc
     9721 gaggtccaac gcgcgcggca ggtccacgaa ccgcgccgtc acgccgctcc cgagcgcgta
     9781 gcggagcgcg ttccactccg gagaaaagcg ggcgaacggg tagtaggcgg cgtcctgtgg
     9841 ggcgtccggc gggtagacga gcaaggcgac gggcggctgc atctggggac gggccatcag
     9901 cggcagtacg gcatctgcgt cggacggccc ttcgacgagg acggcgtcag ggcgcaactc
     9961 atcgagggcc cagaggaggc tccgcgcgct gccggggccg tggtgtcgga tgccgaagac
    10021 ccggatcgcc acggctacac tacttcgcgg cactcgcggt agaggtcctt ccagtccttg
    10081 cgctcgcgga ctaccgtttg caggtactcc agccacgcca cgcggtcctg gacggggtcc
    10141 ttgacgatag caccggtgag gccggcggcc acatcgttgg ctttcatggc gccgtcgccg
    10201 tagaaggcgg cgctggctag gccggcggtg aggacgctta tggcctcggc ggtcgagagc
    10261 gtgcctgttg gggtcttcag cttcgtcctg ccatcctccg tgacgccgcc gcgcagctcg
    10321 cggaacacgg tgacgacgcg gcggatctcc tccagcgccg gcctttccgc aggaagttcc
    10381 aaagctcgac ccaacgacgc cacgcgccgc tccacgatct ccacctcctc ttctgtcgtc
    10441 tccggcacag gcaacacgac cgtgttgaaa cgccgggtca atgcgctgga gagttcgttc
    10501 acgcccttgt cacggttgtt ggctgtggcg atcacgttga acccttttca cgcctgaacc
    10561 tcggtattca attcagggac gggcagggcc ttctcggaga ggacggttat gagggtgtcc
    10621 tgcacctcgg gggcgatgcg ggtcagttcc tcgatgcggc agagcctgcc ggagcgcatg
    10681 gcgttcatca tggggctctc gaccagggcg gcctctgagg gaccttcggt gaggagacgg
    10741 gcgtagttcc agccgtagcg gatggcggcc tcgtccgtgc ctgcggtgcc ttgaataagg
    10801 agcgtcgaat cccccgatac cgccgccgag aggtgctcgc tcacccacga tttggccgtt
    10861 cccggaatcc cgtagaggag cagggctcta tccgtggcga gcgtggctac ggccatctcc
    10921 atcagacgcc cgttccccac gtacttgggc gagacggtga acccgttggg caacttcccg
    10981 cccatcaggt acgtcttgac ggcccacggg gagagcttcc agctcggcgg cttctggcgg
    11041 tcgtcggcct tcgcaagctc cgccaactct tcagcatatt cctgctcggc gtgttggcga
    11101 agcaaggcgc tcggtccatc ggtcacagcg gtatctcctt tacgagcctg cgtcgcaact
    11161 ccagcgtctc ctcgaacttc tctagtttct ctctccagta gtaccggagc acctgaccgt
    11221 cggagaacat cctctctggc agtacttcgt caggctgaag caccgttgcg tgatccagac
    11281 acgagagagc tatccgctcg gcggcgggct gaggagtccc caaccaggga tcgctcacgt
    11341 cgcggttcct tgcgaaggtt cggggtacat gctttcgcaa cttctccaga tacaggaggc
    11401 tcaattcctc gtcccacggc ccaggcaagg cattcaaggt atacgctagc tcatgactca
    11461 ttccactact ccgaagcacc ccaccaagct ccgcgtgtgg aagcagaggc gccagcaact
    11521 ccaatgtcga cgtcaagtcg tgctgtgtct ccggcgcctc gtgccagcgc ctccatagcg
    11581 gccacgccca cgactcttgt ccgtgccgca cggccgcccg tctccagcct gtgagcagca
    11641 cagcctccca ctcaccgcgg gcggcggcga caagctcttc tggagtcgcc ccgaagcgcc
    11701 tctcccagta gacaggcgga atcacctcca gggtacgcac gaccgtccag gctctctcgc
    11761 ccattttcct aggaggcgaa gcctctccgg gcaactcgcg tttccatccc tcgtccacgg
    11821 ccggtgggag ttccaccatc aacttccccg atcgtcttcg acccaggaag ccagtggaag
    11881 gcggctcata gcccgctatg atggcctcgg ctcgttctgc gtacctcagg gcatatgcgc
    11941 tgccggggag gcgtgccagc aagtccgccg ccaccgcgcg aaccttcata ctccggtcgc
    12001 cgagcgagga ttccaggaac ggctcgtctt cgggagagag gttttcctcg aacgcctcca
    12061 gcatcgcgga ccgcgcatcg gccttctctt gccgccacgt ttcggagagc cacgcgcgac
    12121 ccagcgcagg atctcgcccg cgcacgcggc gcagcacgga tgttcgctcc ttcaaatccc
    12181 cttcatccca gacgtcccga aggtctccct catcgcccac cgcccaggcc cactccggat
    12241 tgagccgggc gagccagcgt ccccgctccc ccagcacggc gacgacggcg gggcgcaact
    12301 cctccactac gttcatggca ccggggagca gcgcgtgtgg cagacggagt ccggcgagcc
    12361 gcagcctttc gagggcttcg aggagtatgg acgttctctg gtccacgagg agtcgaccca
    12421 agagctccgc tgctttcgcg gagcaggccg gaatcgtctc gtccggcgcg ggttgaggag
    12481 cttctatgcc tgactctgct aggcgcccgg cggcacggta gacggcccga gtaccagcgc
    12541 gaagcaatag ttcccgttgg ggactcttcc cagtcgcgct ttcgaagaga ccgtccgttg
    12601 ggagaccgct tgcgggtggc gtctcccgag acgttccggc gactgcggcg cgcgtgaggg
    12661 cgtccatcag ccttcctctg ccaggatgca gtaggttccg ccggcggcta cgcccagcgg
    12721 gatcagacct tcaccgtccc actcggcagc gaggtccacg ggccggccgc cggacatgga
    12781 gagtagggtc cagtgttctc cacccgcgag cgggagggcc aggccagcgc ggtcgcggat
    12841 cagccaccgc ccgccttccg atacaccatc caacagaggt acgacccctc tcagggcggc
    12901 cgggaggcgc tcgagccagg gctggagggc tgtcgctttg gaggtgtgat ccaagaacgc
    12961 ctccaccgta tcgtgaccag gcaacccctc acagcgatct ccaccgcccg accttgaccg
    13021 gactaccgct ctcagaggat aggcgccggg atagaaggcg agctctcccc gccacttcgt
    13081 gccagagacg aagccttcgg cgaacggcga gcccgctgcc gcgaattgaa gaaccaatgc
    13141 gtagcggcga gtatcttccc ccagcaacca ggtgcggcgg acggtgaggc gtccttcctc
    13201 ctcggtgcgc tgtccgagca cgatccagcg gtcatcaacc ctctcgccac gctccaggac
    13261 ctcctcctgc gggagcgaga taccgacctc cgccctgacg ctctcgacga gaggatcatc
    13321 gaggacatcg aggcggctga aagctttgga gaggagcgcg agtcgcccga gcccgtcgag
    13381 aagcttcact ggccagtccg gggaggagtt tggcaatccc gagagccgcc ggaggcgcgc
    13441 agcgacgccc ggagcctggg cgtccaccat gcgcttcgcc tgctcttccc agaacgacgg
    13501 cggctgcgtc cctgctgccg cgagaccgcg ccgcaccaga tcctccatcc acaggccgag
    13561 cgcgtccagc ccggcggata tgcgcgaaag ccgctgcttc tcccgccttt ccctcgccac
    13621 cagatcaggc gcggcggcct cgtctcgccc cttcgagcgt ttgccgccgg tcgttctggc
    13681 ggcgcgacgg gcgagccagt ccgtgaccca gtcgggcgag gacgtttctg aaggtgtggc
    13741 ctcgagggac aggaagagaa ggccgatgac gtgtttgcag ggctgtttgc ggctcgggca
    13801 ggagcacttc accgccaggt cggagaggtc tacccgcacc tggtagaccg cgctgcccct
    13861 gcactcgccc caaagcgcct cctgattgcg gccgaggtcc tgccagaact ttggtgcgcc
    13921 aagcttcttg cctgcggcgg ccaccttcgc atccggcgcg agcgccgcga cgccctcgga
    13981 tgtcaggccc gtattcacac ttcccacggc atggggacgt ggtgtcgtcg gagcacagtg
    14041 gtcgaacacc tcaccataga gttgattcta gcggcgcagt ccgggcgaaa acaccgttga
    14101 aaacctcggc aaagcgcgtt tcacggcggc gttctcttgt atccggtccc gccaggggat
    14161 cagaccctga agctatcttt cccgctgccc ggtgtcgccg ccgaagcgcc gctccaggag
    14221 ccactgtgcc catcggtcgc gcaccggctc cgtcatggcc gggctcttta cctgccgacc
    14281 ggcgtcttcg gcccctcgcc tgagagtaca gcgacgatgt tctcagccgc gagcgccgcc
    14341 atcctgtcgc gggtctcgac ggaagcgctg ccgatgtgcg gggcgaggac gacgttttgg
    14401 agttccagga gcttcgggtg gacatgtggc tcctcctcgt acacgtcgag gcctgcggcg
    14461 aagatacggc cctcggcgag ggcgtcggcc agggccgctt cgtccaccac agggccacgc
    14521 gaagtgttga cgaagatggc ctcgggtttc atccttccca actccgcctc cccgatgaga
    14581 tggtgggtct cgccggagag cggggtgtgc accgagacga agtccgcgga ctccagcaat
    14641 tcgtcgagat ccaggtagcg ggctccgagc tcttgctcag catcctcttt ccgggagcgg
    14701 ttgtggtaca gaatctccat gccgaagcct ttcgcgcggc gggcgacggc ttgcccgatc
    14761 cggccaaagc caacgatgcc gagcaccttg ccccacacgt ctcggcccat gagctgcttc
    14821 ggaccccacc actcccaacg tccggcgcgc agcaggcgct ctccctcccc tagacggcgc
    14881 gcggcagcga gcagcaacat gaacgccacg tcggcggtgg tctcgtcgag cacaccaggc
    14941 gtgttggtca ccacgaggcc cctctccgtc gccgcctcaa cgtccacgtt gtcgtagccg
    15001 acggccatgt tggcgaccac cctcaagtcc ttaccggcgg cgtccattag ctcgccgtct
    15061 actttctccg ttgcggtcga gagcacgccg gatacgccgc ggacggcatc aagcagctcc
    15121 tcgcgctccg gcggacgctc gtgcaggatc ctcacatcga aaggctcgag ggcgcggagc
    15181 ccggcggccg ggatctcacg tgtaacgagg accttctcgg gcatggctcc tccctatttc
    15241 gggctctact cttcgagcat ctgtcgcaat acggtctgca agatgccgcc gttgcggagg
    15301 tactcgacct cgaccgggga gtccacgcgc gccttcacct tgaactcctt cgcttcgcct
    15361 tcgtcgcccg tgaccttgac ggtcaactgc ttgccaggct cgaggcccgc aaggcctgag
    15421 atgtcgaacg actcgcggcc gctaagcccc agggattcag cgctttcgcc ttctgcgtac
    15481 tgcaagggca gcacgcccat gccgatcagg ttagagcggt ggatgcgctc gaagctctcg
    15541 gcgataacgg ccctgacacc gagcaggaac gagcccttcg ccgcccagtc gcgcgaggag
    15601 cccgacccgt actctttgcc ggccagtacg acgagcggca cgttctccac ttcgtacttc
    15661 aggctggcct cgtagatcgt ggtctcttca ccgtcgggga gatggacggt gtagccgccc
    15721 tctttgtcca cgagctggtt gcggagcctg atattgccga acgtgccgcg caccatgact
    15781 tcgtggttgc cgcgcctgga gccgtaggag ttgaagtcgc gcgggtgcac gcctttctgt
    15841 ataaggtact gccccgccgg catcttggaa gggatggcgc cggcaggcga gatgtggtcg
    15901 gtggtcacag agtccccgac cttcacgagg acgcacgcgc cttctatgtc ctccaggtct
    15961 tcgggttcgg gtcccatgtc cttgaagaac gagggctcct ggatgtaggt cgaatcgggg
    16021 tcccactcgt agagatctcc gctaggcacc tccacgtcgt tccattgttc gttgcccgtg
    16081 tacacatcgg cgtactgctc cctatagatc tcggggtcga gggcgttctc gatctccctg
    16141 gccacctcct cctgtgaagg ccagatgtcc tggagataca cgggggtgcc actatcgtcc
    16201 tcacctatag ggtccttgga aagatctatg tccaccgtgc ctgccagggc gtaggcgacg
    16261 acgagtggcg gggatgccag gtagttcgcg cggacgtcgg ggttgatgcg accctcgaag
    16321 ttccggttac ccgagagaat ggcggccacg accaaatcgt tctcctctac ggcggcggag
    16381 atctcctcgg ccagcggacc cgagttccct atgcaggtcg tacacccgta gccgacgacg
    16441 tcgaacttta gcttttcgag cggatcgagg agccccgaag tctgcaggta ctccgtgacg
    16501 accttggatc ccggcgcgag gctggtcttg acgtgcggcg cgactgagag ccccttctcg
    16561 acggccttcc tggccaagag gccggcaccc atcatcacgg aaggattgga ggtgttggtg
    16621 caactcgtta tggcggcgat cacggcggag ccgtgcctga gatagagttc ctctccgtcc
    16681 accgtgacgg tgatggcccc cgtaggttct gcctcaggct tgccattctc gggctcgccg
    16741 ccggccgtgg ggtctccccc atcctcattg ctcacgccag aggcgcctgc gggagtgtcg
    16801 ctcgcaggaa aggactcggt gtcgtccgcg gtgtgactct cacccgtgga gatggcgccg
    16861 ccctccttgc cctcggcctc gttggtcccc ccgcgggcga ccggtatatc agggttcggg
    16921 gtatcgctcg cggggaagga ttcttcgtcg gcatggttgt aggcgtcctc gtaaccgttc
    16981 cccaggagtg ggtggtcggg gtccaccatc tccgccagcg cctgcctgaa ggagggctgc
    17041 atgtcgtcga gggcgaccct gtcctgcggg cggcgcggcc cggcaaggct agcctcgact
    17101 gactccaggt ccagatccag cgtctccgag aagcgtggct cgggtgtctc gtccgttcgc
    17161 cagaggccct gctccttgct gtacgcctcg accagctcga caagctcctc atcacggccc
    17221 gtgccgcgca ggtagcggag cgtctccgtg tctacgggga agaacgtgca cgtcgcccca
    17281 aactcggggg acatgttcgc gatggtggcc cggtcgggta gcgagagctt gctgagacct
    17341 tcgccgtaga actccacgaa cttgccgacc acgccgtgcg agcgcagcat ctgggtgacg
    17401 gtcagcacga gatctgtggc cgtgacgcct tccttgagag cgcccgtgag cttgaagccg
    17461 atcacctcgg ggacgagcat gtagtagggc tggccaaggg tgacggcctc ggcctcgatg
    17521 cctccgacgc cccagccgag cacgccgagg ccgttgatca tggtggtgtg gctgtcggtg
    17581 ccgacgagcg tatcgggcat cgccactcca tcctttacga tcacaccctt cgccaggtac
    17641 tccaggttga cctggtggat gatccctgta gcaggcggca cgacagagaa gttgtcgaag
    17701 gcttgctggc cccacttcag gaactcgtag cgctcgcggt tgcgctcgaa ctcccgctcg
    17761 gcgttgaact ggagagcagc cggagatccg aaagcgtcga cctggactga atggtctatg
    17821 acgaggtcgg ttggaacgag cgggttgatc ctgctcggat cgccccccac gttcgccatc
    17881 gcgctccgca tcgctgcgag gtcgacgacc gctggcacac cggtgaagtc ctgcatgaca
    17941 acccgggcgg gcaggtacgc aagctctccg ccgaccgact cgggccattc ggccaacgcc
    18001 tcgacgtcct ctttcgagac gaactttcca ccggcgttgc gcaagagaga ttcgagcatc
    18061 acccggatgg agaaaggcag cgagaacacg tccccgtcca cattcttatc cagggtcttc
    18121 aagctataga gttctacatc tccggaacca gtcttcagcg tcgtgcgcgc accgaaggga
    18181 tcgttcgcca tcttcgtaac ctcttctccg aaattcgtat gcctgactct gcccgattat
    18241 agatgatccg ggatgcccta cgagacatgc gcacctctag cgcagtctac ccgcggcttc
    18301 ggggaacgag ctcgtagagg aaaccatccc tctcgaccgg tacaacatcg aaacccgcct
    18361 cgcggaagga gtccattcgg gcacggccgt tagccagtag gtagacgggg cccgtctgtg
    18421 cggcctcgcg ggcggactcg acaccggtgg tgtcgtcggt gccgtagcga tcggcggcct
    18481 cggcagcgct gatctggtca ggccagacga catcggtgtg gcggtcccag gatgtacaga
    18541 aggggtccat cagggtgaga tcccgtcttc gcccctccac gagcaccatg taccagagag
    18601 ggctcctgtg atgcagcacc gtggcgtcct tctcgacatc gctgaccacg gccccgatca
    18661 tccttcgtcc tccataatcc tcgctacggt cgtgcgcggc gtaggagccg ggcacgccaa
    18721 gaagtggtac gaggagcatc agggtcgaga ggattacgag aagggcacct ctggcggtag
    18781 agccgatggt caggctctcc accaatcgca gcagtgctcc aaggcccgcc gagatgcaca
    18841 gtccgaaagt cagaattgcg gggatgagga atacgtagaa gtcctcgatc gccaactgca
    18901 ggtaaacagc ggcctgaacg agacacccca agaagagcgt gccaaggagc atggccgtcg
    18961 cgcggtctgc aaagagcaga tagacggcag ccagtactcc gaccagcatc aggacaagag
    19021 gaatttgccc gagtagatgg tccccgaata gccccagttt ggtcgaggtg tccgcgagcg
    19081 tgaggctgga agggctgcag tgcctcccct tctctgaaga ttcggccaga aagctgccgc
    19141 ctgtgacgag tagcaagaag cgccacgggg tcgaagggtc agcctcgttc agaggcgcgt
    19201 gcatcagagc ccgtatcggg aggtaaagga gcggaaggag gccgagcagg aagagcccga
    19261 cgcccttcag tatcagcccc ttcctcgaaa acacgcgcct gtcggtaaag aaaacgaagg
    19321 ccagtgtggc agggaccagc agaacgctcg tcaggtggtg ggtgagggag agaccgacca
    19381 gaaaggcaga gagcagcagg tatcggccct cgcggcggtc tctccagagg aacaagaaga
    19441 ggatgaccag cgcaacgagt agtgcctcga aggtgtagac ctcggcgatc acagcctggc
    19501 tccagaatgc tcctgagagc ccgaaagcca gagcgccggc cgccgctgcg acggcccgtc
    19561 ttgccaaccg caaccccgcg aggtacacga ggagcaccgc cgcgacgccg tagacggcgg
    19621 aggcgaggtt cacgcggtag gcggggtccc cgaatggcag atatgtgaag aagtgtgtca
    19681 gcatcatgta ggttgggtag ccggtcggat ggcctacccc aagcaccggg accgcggtct
    19741 ggagcatcgg ggagtcgagc gtgtcaggag ctccgtaagg caggaccgtc ggtgccagag
    19801 tcccgatgta gaggatgccg acgaggaccg cgaccgctcc cccgagcaaa gccgcgccgc
    19861 cctgctcgag atatctgatc ctgtcgctgg aggagggatt catcgcagtg cagaatacta
    19921 cgcacaccgc cctacccgat ggtcctctgt atactcagcc cgtggctaag cgtggctaca
    19981 cgcgtgacac catccgaggc ggcgccagcg agtgggatgt ctcgcggcct gtttcgagct
    20041 acgcgcgaac gttggcgatg ctactcgtgc acccggtcag gttcttcgag ctcttgccaa
    20101 ggattccgga catcagggcg cctgccctct ttctcatctt ctccggcctc cccaccgcga
    20161 tcttgtggct gcccttctgg gggctccctc ctgcgctagt agcggtcgta ctgccactgc
    20221 cgctatcctt cctcctcgcc ggcctctacc atctcggcgt cctcggtggc cggcacgggt
    20281 acatggtgac ctggagggtc ctagcgtacc cgctcggttt ctacctgccc ttcgccgcag
    20341 tccctatgct cgggcctctg ggagcggctt actcagggct ggtgatcata ccgctcgggc
    20401 tcgctgaagt acatgaggtc ggaaggctta gggccgtgct gttctgcgcg gccatcgggg
    20461 tcgtgttcgg ggcggcttac tacttactga gcgttgcctg atccaggccc gaccaaggtt
    20521 cattcttcgg ggtcgggcgg gtcttcctga gaacctacga actccagcga gaccacgtcg
    20581 gtctcgacga agcccagaga gcggtagaac tcgcgggccg ttacgttcgc gcgggtggcg
    20641 gcaagttcga tctgggacgc gtcgttgtgg gaagcgacgt tgcgtacctc ctccatcagg
    20701 agcttgccca ccccggcccg tctgtgatcc tctgcgacga cgagcatcgg cacctcgacg
    20761 acggtgtcgc cgtgggcgag gtcgggtttg atccagaagc tcaccccccc cacgatcccg
    20821 gcctcgttct cggcgacgag gacctgcgcc ctgggttcgt ccaggagctc ctcgagcctc
    20881 gcccttatgg cctcttccgc agacggcgag tcaccgaccg tctcggccag ctccctggcg
    20941 agactgcaga tctctgcagc gtcctcgact ttcgcctctc gcaccttgta gttggagccc
    21001 atgctactcc tcagctcgtc gtctgaagta ttacttaccc tacgtagccc ggcgacgaac
    21061 ggatgaaggc gctaagatga tcgaggagca gaaaggaggt tgatgggaag cagggcggtc
    21121 gtgatcgtgc tcgacggcct cggcgcgggc gaagccccgg acgcggcgga cttcggagac
    21181 gagggcgcca acacgctggc gaacacggca aggtccacag gcggcctccg agcgccgaac
    21241 ctccaggccg tcgggctcgg taacatcgag gagatagaag gcatagctca ctccgcctct
    21301 cctgcggctt cccacggcct gatggtcgag ctatcagcgg ccaaggccac cctcgcagga
    21361 cactgggaga tgatgggcct cgtactcgaa gacccgctac ctacctaccc ggaaggcttt
    21421 ccagaagacg tgctgcttcg cttcgaaaac gagaccgggc gcggggtcat tgggaacagg
    21481 ccggcctcgg ggactacgat catagaggaa ctcggccccg agcaggaaaa gacgggggcc
    21541 tggatcctct atacctcagc cgactccgtc ttccagatcg ccgcgcacac cggcgtgatc
    21601 ccactagatg agctgtatga ggcctccaaa aaggcccaca agatgctcat ctccgagggc
    21661 gagatcatgg tcgaacgggt catcgcccgt cccttccacg ggagggctgg cgcctacgtc
    21721 cgcgaaaacg agaaccgcca cgactacggc ataacaccac cctcggagac ctatctggac
    21781 cgcataaagg acgcgggcca cgagacagtg gccgtcggca agatcaagga catcttcgac
    21841 ggccgtggca taaccgagca tttgcccgcc ccgcccgacg atgcggccaa agtagacgcg
    21901 atcctggatg cgctgggcca tgtcgagtcg ggtttgatct tcgcaaatct cgtggacttc
    21961 gacgccagct acggccaccg ccgtgacccc gagggtatgg ccgcaaacat ccaactcttc
    22021 gacgagcggc tgcccgagtt gctcgatgcc ctaaccgcag aagacctcat gatcatcacc
    22081 gcagaccacg gcaacgatcc caccttcaga ggaacggacc acacccgcga gagggtgccg
    22141 ctcctcgtca tgggcggcgg tgatacacgc gacctcggga ttcgcgaaag cttctccgat
    22201 gtcggagcct ccgtcgccgc gtggctcgga gtcgaaaaag gcgggctacc gggcaggagc
    22261 ttcgtgtagg gagccagaga ttcgtccacc cgctagaatg gcacgacagc agcgctgatc
    22321 caaggcaggg accgtatggg ctcagggaac gcgatactcg atgccataga ggccaagaag
    22381 ttcggcgggg aattatccca cgggctgatc caggacgtgg tagaaggcta cgctacggga
    22441 aaggtgcccg actaccagat ggcctcgcta ctcatggcga tcttcatcca gggaatgagt
    22501 tatggggaga cactcgcgct gacacgggtg atggccgaat ccggagagat ttactccttc
    22561 cctgagtgcg tggacaagca ctccacaggc ggcgtcgggg acaaggtgag cctcactgcg
    22621 ctccccatag tggccgcttg cggggcaccc gtagccaagc tcgcggggcg gggcctcggc
    22681 tcgtcgggcg gcacgataga caaactggag tctatccccg gcttctcgtg caacctctcc
    22741 gaggagcagt tcaggaacca ggtcgaggag gtagggctcg ccataatcga ggcgggggat
    22801 ctcgcgccgg cggacaagga gatctatgcc ctccgcgaca cgacaggcac ggtagactct
    22861 ctgccgcttc tcggttcctc gatcgtctcc aagaaagtcg ccaccggcgc ggggcatctg
    22921 ctctacgacg tgaagcacgg ctcgggagca ttcatgaaga ccatagaaga ggcccgccag
    22981 ctggccgagc tactcgtacg cctgagcgag gaccttggca tcgacgcatc ggccctgatc
    23041 acagatatgc acaatcctct ggggagcgcg gtcggcaatg ccctggaagt ccgcgagagc
    23101 gtccgcctac tcaagaacga gcccgtcccc gacgacctcg tcagcgaagt caaccacgtc
    23161 gccacccatc tcctgacgca aaaaggcatc ccggaagcgg ccgctgccgt cgaaaatgcg
    23221 ctctcctcgg gtgcagccta cgagaagttc cgagcgttcg tcaaggccca gggcggcgac
    23281 gcagacgccc tcgaagacct ccctgtctcg gccgaggtaa gggaagtaaa gtccccgcgt
    23341 ggcggtttcg tagcccgttt cggggcgtcg ggcatcggca gcgcagccct cgtcctcggt
    23401 gccggccgca agcaaaaggg cgaccgaatc gacccaggct ccggcatcga agtcctcgta
    23461 aagccaggag accctctcca acagggccaa cccgtagccc gcctctacgg cgaacgcgaa
    23521 gcagagctgg ctcggcgcct cgtagtagag gcactcgaga tctccgacga gcccgtagag
    23581 cctccccctg ttattctgga tagcctgtga aaatagcatt tcagcacgcc gccaggcttc
    23641 gccaggcggc ttgtcagcca ggctatgaat gacggaggga tggttccttt gagagtggtt
    23701 tatgaaggca agcagttcga ttcctcgcac gggcggcgaa ccggcaccag gaagctgaca
    23761 agctgaaatg ctgacaagcg cccgacaggg cgcgtgctga agtgctgagg tgctgaagtg
    23821 cttagacgac cgaagggagt cgaccatgtg ccctgttcac gtgcgcgcgg agcctggaga
    23881 ttttgcggag agcgttctct tgccgggcga ccccctgcgg gcgaagtaca tcgccgagac
    23941 gttcttcgag gacgcgaagc tcgtgaccga agagcgcggg atgctcggct acacgggaac
    24001 ttacaggggc aaaccggtct cggtccaggc cacgggtatg gggtgtccga gcgcctccat
    24061 cgtgacggag gaactgatac agctcggggt gaggaacctg ctcagggtcg gcacctgcgg
    24121 agggtacagc agggagctac ggctggggga tctcatcgtc gcgacctctg ctgccccgca
    24181 ggacggaacg gtctcctcga tcacccgcgg cgtcccatac gccccggcgg cccacttcga
    24241 cgttgtccat gccgcccacc atgcggcaga gagcgcgggg cgaaggacct tcatcggcca
    24301 ggtagtcagt tccgacctgt tctacgaccc caccgaagac cctgcgacat tgtggggcgc
    24361 cctcggggtt ctcgccgtcg agatggaggc cgcagcgatc ttcaccatag ccgcaatgcg
    24421 cggcgtgaag gcgggctgcc tgctgaccgt ctccgacacc ataggcgctg agatagtcag
    24481 gataagcgac gatgaactgc gtaacgcggt ggataacatg atggcactag cgctagacac
    24541 cctcaacgcc ttaaactagt acctcctgtg gggacgaggt ggagcaggtc aggcacctac
    24601 ctccgctaga taggaacgta gagtgacgat cctcaacgag tcggggatac cggacgttct
    24661 cccccctttg catgccgtaa atgacctggc tcagaggctt cctgatgtcc tgatcgtcat
    24721 cgccggtaca caggccgaag cgcaccttgt ccagtgcctc ccgtcagcac catcccctaa
    24781 cctcccccgc tcccggcgca accagcgggt gatcttcgct gtcctcgacc ccgacgaccc
    24841 gccgcagcaa ggccttctgg cctctcgcat cgtagagatt gccgccggac tccgtggcgc
    24901 gaggctagtg ctgctcgtgg cgggacgagc ggcggaatcg ctcggtgtcg acgccgcgtt
    24961 cgaggccagg ctcgtcaccc gcaagctcga tgtgccggcc gaggtggtga atccagatgc
    25021 accccacgaa gtccctggca ttctctccac cgacctagag gaccgtgtcc tcgccgccct
    25081 ggtgaacctc tgcccgaaga ggaagacctc agatctcgta gagaccgcct ctggggcgaa
    25141 gcgtggcggg ctcttcggca gctttctaag acggggcacc gaaacatcgg gctccaatgg
    25201 cggccgcccc gtcgtgctgc tcggaggcgt cctctcaccg ggtgccgatc aggtgctcgc
    25261 cgcggaactc tccaggaccg gggtggaggt agtagggagc gtccctggat ccgggatagg
    25321 caagcttcca cccctcgggg aaggcacggt ggttgccgtg atggatccga acctgtcctc
    25381 agcggcaacg gtcgccgaga gacgcggggc cacggtggtc aagactctca tgcctgtcgg
    25441 agtcgacggc acggcacgtt tcgttcagga cgttgcggag gaagccgggt cgcggaccag
    25501 cgaggtagtg cgggctagat ctgtgtggga ggatctaggt ttcttgagga accgcataag
    25561 ggggaagagg atcttcttca ccggcgacac gggactggag atccccctcg cccgcttcct
    25621 cgccgacgcg ggggctgtcg tactcgaggt tggcgcaccg cggctggacc gcaggttcct
    25681 ggccgccgag ttgcaggtcc ttggccccga cgtcgacgtc gtcgtctcgc cggactacag
    25741 gggacagacg aatcggatcg acgagagtcg gccggacgtg gtgatctcca gccccggtct
    25801 gcaactgccc ctggtcgccc gcggacacct gtgccgctcg ccccgtgact tcatcgcggc
    25861 cggtctctac ggatacggag ggtcccgtcg catcctcgaa ctcctggtgc gtaccatgga
    25921 gcgcgcagag accctcgact cactgaacct gtgaagctgc tgcgcggcgt ctacgaggga
    25981 ccgcagagct acggtatctt acgggtggcc gcgagcatcg ggggcgtcca caccttgctc
    26041 agggctttac ctggcgaagc gtacttccgt acgctctacg gggccaggga gcgaaccggg
    26101 gcgatggcgc cggtcacgct cagcccactg cgcgaggggc caggtgactc gatggcgcca
    26161 gggaatctcg cccgcgacct ctctggtgtc gtccggcgtc acccgaaagc ggagatcttc
    26221 atcgtggccc gctctgaggc gtccctgctc tcggagaatg caatcaccaa tctccccgag
    26281 gcagaagcga cagggagacg ccccaaattg gtgacgttcc cctgggaggg tccgggcatg
    26341 agggagatcg aggccgcaga gctcgcgctg gaggacctga tccggtccct gatgcaccac
    26401 aggcccgaga ggagccctta ccctacggtc aacctcttcg ggccaccgat cttcggacca
    26461 ggcgcggcgg ccgagtacgc cgaggccgag cggctcctcg atatgatcgg ggtcgaggta
    26521 aatgccaggg tgccgctggg agcgcacgct ggagacctcg accgcctccc acgcgggtgg
    26581 gcaaacgtgc tcctctacag ggaggtcggg gagtcagcga cgctctacct gcaagacgtg
    26641 ttcgggatgc cgcgggtgac gacccctatg atcggcgccg caggaactgg cgcggtcttg
    26701 cggtctgtcg gcgaattgtg ccaactcgac ccgaagtccg tgcagcgtgc gatctgggcc
    26761 gagctggccc gcacggcgaa gctcccttgg tacgcgcggc tggaaccacc cgagaccttc
    26821 caagaccgca gggtcgccat attcggggac ttcacttacc ctcttggcct cggttacgcc
    26881 ctggcccgcg aggtcgggct ggaaattatc gcctgcggga cgtatctgac tcacctggaa
    26941 agggatttcc tcttccacgc ccacacgttc acagaagggg ctttcgtcga agacgatcct
    27001 gatcaggtcg catcccgtat cgaggccgcc aagcccgacc tgatcatcgg gacacatctg
    27061 gaagaggacg tggccgactc cctcggtgta cccttcctcc ctctctgccc tcctgtcact
    27121 gacaggccct tcgtcgggcg accgctcatg ggctacgccg ggtcaagcgt tcttgccgac
    27181 gcgctcgaca acgtcctgcg agagataggt gtggagccag caggggaggc cagcatacag
    27241 tggaccgacg gggcgttgca ggaggtatca gaggtgccgc tattcctgcg gggcagggcc
    27301 cgcagactcg ccgaagagcg tgcccgcaga cagggctcgg ctgaggtgac gcgagagatc
    27361 ttcctggagt cacgctcctg aaccgatata atcctgcccc gtgggtatca gggactttat
    27421 acactgcctc gatgaacacc agaaccggct gaaccgcatc cagagcctct cgaacaggga
    27481 gcgcgaggag acattgaggg aggcggagac gatggtcggg atggcggatg cagccatcaa
    27541 ggaacttgag tcggtcctcg gaaacaggcg ggtcaacctt cccgaagag
//
LOCUS       DASTKM010000009        19470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_882340, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000009 DASTKM010000000
VERSION     DASTKM010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 19470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19470
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882340"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFQ10_00265"
     CDS             complement(<1..298)
                     /locus_tag="VFQ10_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9963798.1"
                     /translation="MELVSLDGVMESPEEWAFSYSNAEMEEANASEMAASDAMLMGRV
                     TYERMAAYWPNQPGGTPMVDFLNGVPKYVVSTTLDEAAWSNAVIHTGAIGPVYAD"
     gene            608..880
                     /locus_tag="VFQ10_00270"
     CDS             608..880
                     /locus_tag="VFQ10_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963799.1"
                     /translation="MAQDHQEGLRSREGLCEETLEVALCYGWIDSQAGRFDDQYWLLR
                     FTPRRPKSKWSKGNRGKVRKLIEEGRMKPAGLREVERAKAEGRWEQ"
     gene            849..1679
                     /locus_tag="VFQ10_00275"
     CDS             849..1679
                     /locus_tag="VFQ10_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET9963800.1"
                     /translation="MPKPRAAGSSSLVAAANTITEERRLERHIGTIARWSTGSEHMVA
                     FTGAGISTDSGIPDFRGPEGVWTRRDAGLPAPRWRVPPGEVAPNASHLSLFELQRLGK
                     LRFLITQNTDNLHRRSGLNPEFLAELHGNGQLLRCLDCYRLYTRQEVGWDRGRWGPAY
                     RTQKPVPGQPECADCGGRLISSVVNFGDPLPETELMLADHHARRCDLMLVLGSSLMVQ
                     PAASLVGLALRSGARVVLVNRGKTPYDRAATLRVWRGIGEVIPPAVERVREAIGDQKE
                     "
     gene            1897..2982
                     /locus_tag="VFQ10_00280"
     CDS             1897..2982
                     /locus_tag="VFQ10_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963801.1"
                     /translation="MNTPAYVFWPELTSLAVGYSVIGAIIASRLPNHPIGWICCAIGL
                     IVAVDHFAGEYAVYALLAHPHGLLGGRAMLWLQGWFWMLFIGLIVFLLLLFPTGRLPN
                     RRWRTFTWVSVAVISAAMIWSSAISPDVGFNAPPSPVQLSVLLLGGVAAGSVIVGRRN
                     ARGIELQQIKWLLYVGPLFFIAAGLQIGFYYFWLAERSWGLWASYLLVAIGGLGGPIA
                     IGIAILRYRLYEIDILINRTFVYGSLTATLIVLYFGGIVVLQRVFVLLTGQQSTLAVV
                     ASTLAIAALFSPLRRRIQGFVDRRFYRRKYDARKTLEAFSAKLTSETDLDALTDDLIE
                     VVRETIQPAHVSLWLRPERASKGRQTD"
     gene            complement(3111..4616)
                     /locus_tag="VFQ10_00285"
     CDS             complement(3111..4616)
                     /locus_tag="VFQ10_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9963802.1"
                     /translation="MRKRTLTRRDFLKMTGAGMAGATLIGTTGCDLPERIRNITLDPP
                     GVKEDTNVVLIIIDSLRKDHIGVYGNDKIRSPNLDALAKDSLLFTKAYPESIPTICAR
                     RAIHTGLRTWPFRDWVPPKGEDIILQGWEPIPNYQTTLAEMMQAAGYGTYFVTDNMHQ
                     FKPSYNIHRGFDVFDFFRGQTTDNYRPAWTYPKDKVAQALLKGNIPAMTGQMRQYFAN
                     VKERKTEADWFSPMVFSQAADYLEVLSKGGPFFLTVDSYDPHEPWDPPERYVEMYDDE
                     PYNLKEPFSVIYGPSSYLLPRELERMKARYSAEVTMTDRWLGRFLDKMEELGLFDNTL
                     LILLSDHGVAHGEHGYTGKPDNVLWPEVTDIPFYIRHPEGKGAGQTSGYYASTHDVAP
                     TILGFLGIEPRQELDGQDLSVVLGGGKPEARPHFTIGYNDHVWARDDRYVMFSTNQGA
                     DAKLYDVEQDPGMHNDIAGVEPNVVKRMFEGYIIRDAGGPLPTYDYGTAAG"
     gene            4724..5158
                     /locus_tag="VFQ10_00290"
     CDS             4724..5158
                     /locus_tag="VFQ10_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963803.1"
                     /translation="MRSIMAVGIGLLLALVLGLLVVQGIFAPVLTRFFGLERAGPAAL
                     PLILLVFAAAFSFYFGGMAASYKAPFLHRLHGVLVVPIAFVLSLVLNFVLGKGFLPGV
                     DGTWAAGLVAGFLVVSGTASYVGALRGEALYAHNQKVARRHR"
     gene            complement(5165..6988)
                     /locus_tag="VFQ10_00295"
     CDS             complement(5165..6988)
                     /locus_tag="VFQ10_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963804.1"
                     /translation="MSEDPRDKAAALAEGMEAASALIENLEQEVAALRSDLDQAASAL
                     KAAQEEVSFRAGALEEKERARVEAESEAEDLRAEISNLKQRYSDEQLRLSNEHINELA
                     EVRNRLEEQRRTDVDAASSETRLDTIKEEGRREREALEARYREEIEALKNASEHWEEQ
                     LRTSYQEQEARHAAELESARSAAAERENALEHSVREDFERRLAEERSAADDRQTAAVQ
                     ALRSAAAERELELQNVVGTQQEEIESLRAELEAASRSSEERRKEELRQFKALAEAREN
                     DLRKTHATRLAEAKESADKRVAAIRAQREADNRALRARHEQETARLRREHEEQLVAED
                     ERRKAETWALDERLREAAVQRETEMRAYTARLTELEAARLAQKSTSQEDLERVVERFG
                     AEISDFENRVTELEGALEESEARRNELETVLGEIRASSEESSGITARPDNAAHGEPKG
                     PLEDIEAQNILAEEKVEDLEARLSEAREESQRNAEELQRALESLDRLSDPARRLREGI
                     ALFNESEHARTVASISKAFGLPRVHAALDDTAPGRPTLTFLWGDMAWRRYVSDPTDGV
                     EEPRVYLTGTGEDPAEVEASSRQPNARMDSRGRLMIGVQAR"
     gene            complement(7068..7490)
                     /locus_tag="VFQ10_00300"
     CDS             complement(7068..7490)
                     /locus_tag="VFQ10_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET9963805.1"
                     /translation="MLVPDVREKLRRSGYTLTSQRRAVLEALLGAKGHPSAEDVYLIV
                     KRENPRVALGTVYQALSVLEEIGVIGSKHWAESPTRYDLNVEPHLDIRCIRCGEVSEV
                     PGVALGGVEEIIRNNTPYRVTRATMVLEGLCPACSDSE"
     gene            7598..9286
                     /locus_tag="VFQ10_00305"
     CDS             7598..9286
                     /locus_tag="VFQ10_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET9963806.1"
                     /translation="MFADVLVNLWRLIRNARARLLGRPPEYVWIEVGGPLAEFETPVG
                     LLRRRLSRGPSPPTLERLRLWLDRISADGRPRGVVLRIKKLDAGWAAIEELRREILAF
                     RSRGGRVVAYFADSADIRSYYLACAADEILATPLADLNVVGMRARVDFLKDALHNLGL
                     EVEVVAVSPYKSAGERFVSNDFSRESREQAGRLLDSRFEEVVGAISEGRGLSREEVRA
                     KIDLAPYGAPEALSEDLLDGVLYEDELPTRFGSEERHARLAEWGLAQGSLRMPYRRRS
                     RKKVALVTLSGAIVRGRSRRLPLPLPFVGGEQAGSESVVAALRVAEKARRVAAVLFYV
                     DSPGGDALASDLIWREVERLSAKKPVVVLMGNAAASGGYYVSAAASHIVARRGTLTGS
                     IGVLAIRPVALGLYEKLGISPVALERGARAGLLDPSRRPSPDEMRVIEGQIGRIYTEF
                     KDRVARGRGNDLTDLEGMAGGRVWTGIEALELGLVDETGGFRDALSKARELGGIDRDV
                     PGVLTKVNPPRGVRPLPPEPAQTLLDALGEMWLALSELRAGGVLALAPYEISEG"
     gene            9329..10495
                     /locus_tag="VFQ10_00310"
     CDS             9329..10495
                     /locus_tag="VFQ10_00310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9963807.1"
                     /translation="MSEIPNKGVDRRAMAVLSAGHLFTDLNQGAVAALLPFLIADRGI
                     SLAAAGAILFAATVSSSIVQPLFGIFSDRRPVPALMPLGVLLAGCGMALVGLAPSYPL
                     ILLCVVLSGIGVAAFHPEAARFANYVSGSGRARGMSFFSVGGNAGFALGPALATPLVI
                     LFGLPGAVFLAVPAVIMAAVLFHELPRMLGFRPEAVESERETVANAPEHWGPFVLMIG
                     VVTVRSFVYFGLVAFVASYYERVLDVSPALGNTALTLMLFGGAAGTLTMGPLADRFGR
                     RTVLVVSMLVLPPLVFCFTLVGPFFGMAVLILAGAATVGTFGITVVMAQEYLPGRIGL
                     AAGLTMGLSIGLGGVGAPLLGLVADNAGLSLTMLVIAVLPLPGLILALTLPRGK"
     gene            10618..13182
                     /locus_tag="VFQ10_00315"
     CDS             10618..13182
                     /locus_tag="VFQ10_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9963808.1"
                     /translation="MSVFHFIGVFAYRFRWAILMFWGMVLISSSFFAPGLSGQLKGGG
                     FEGANSEAERVQDLMSGEFGLSPATLTVVFTGDGIPARSEEFQRAQEDALAGVRKLDD
                     VRQVISYAGSKDSRFISEDGEKSYAVVTSDVSIDATRNVVDEVRSKVRSDKLDTYVTG
                     APAVYQDLEEASNEDVKDAEKYAFPFAVIILVFAFGTLVAAGVPVLIGGASVVTALAA
                     LYFIAGIYDMSVFTLTLSTMLGLGLGIDYALFFVSRFREELEDYPTVEAVPRTVATAG
                     RSIFFSGTAVLIGLSGLLFFPFMFMRSIGVAGVVVVFVSVLAALTLLPALLGVLGPRI
                     NRLAIRHRSDGAGSAFWSRSAEVVMRHPLTVILLAAAILGTLLYPVGHMRVGIPEATV
                     LPQEYESRAGDDILKRNFEYAALNPMEIVATFVDDPLSVRGLSDTRELGESISGADGV
                     SRVESAYTIAAAAAEDYAGRVADARNQARKEAAARVDGLVQRQIEEQTLNETDRAVNE
                     ELSQIEATYGAVPPGTEERLRAEIRPRVEREVRSSEGRIRDEVERRLAKEVDRKVPGL
                     PEGISADGKVTPEGVANFLKLPAARQSEDLKDTLDTYVAGDRTLLRGVTEANPYTQDA
                     YGAVDAVRAVEAPDGMSFLVGGLSAGQKDFISSLYSKAPLAAAFVIGVTYLILLITFR
                     SIFIPLKAVIVNILSLTASFGAMVFVFQDGHLSGLLDFTPLGFVDATLPILMFCTIFG
                     VSMDYEVFLLSRIREAYENGDSNTASVAKGLVSTAGIITSAAAIIIVVTGAFALTGIV
                     LTKAIGLGLAVAVFVDATIIRILLVPATMRILGDWNWWPGGRKVTFGARPNEPARD"
     gene            13283..13696
                     /locus_tag="VFQ10_00320"
     CDS             13283..13696
                     /locus_tag="VFQ10_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET9963809.1"
                     /translation="MLDFERILKDRGYRLTNQRRIIVHELEGERHLSAEEIYDRVKGE
                     HPELGLSTVYRTLDLLTELGIVRKDDFGEGYSRYELATERMHHHARCEQCGKVIEFNE
                     ELMEYLALQVERETGFVTDWHEITLYGRCADCAVS"
     gene            complement(13693..14472)
                     /locus_tag="VFQ10_00325"
     CDS             complement(13693..14472)
                     /locus_tag="VFQ10_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET9963810.1"
                     /translation="MRLKGVPLKITVVGSGTVVPRLERRQSCVVVRAGGQMLVFDLGS
                     GALRGMLRSGLDPLSVDRIFITHFHPDHTVDVVPLLFAINYGAHEKRTLPLYVTGPEP
                     FLQFWSSVMKVWGEWMAGDYATLVSELPHECRSPIDLAGCRLSWAPAEHRSESIAYRM
                     DAEGGALVYTGDTEYSRSLVELARGADTLLIECSFPAEVPVPGHLTPEGVARIASEAR
                     VRRVVLTHIYPAADELNLVTEVGRGFEGEILVAEDGLAFEV"
     gene            complement(14483..15148)
                     /locus_tag="VFQ10_00330"
     CDS             complement(14483..15148)
                     /locus_tag="VFQ10_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9963811.1"
                     /translation="MSPLITCKDVKGDNRLILLLSTAAFLVCSAAAGAGLFRPLDVLI
                     LDLAQRTDSGILDGVGMVTSVVGGVEFVAVATVALAAGLLVEERGRLALRLVVAFVFT
                     GLIELAAKTVVPQTPVPGDAMRAPDPSLLDVSTPYPYPSGHMLRSVLLLGAIYLLWPS
                     RPGRVALLIFLVASATSRVYLGTHWPSDVIGGALLGMAGLAWAFEEQPAMKHSTCQNR
                     EKT"
     gene            complement(15292..16176)
                     /locus_tag="VFQ10_00335"
     CDS             complement(15292..16176)
                     /locus_tag="VFQ10_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HET9963812.1"
                     /translation="MKANFEKRKDNQKIERESETPELLAKYLAHIGQGNLLTHREEID
                     LSVRAKAGDRRARQRLIEKNLRLVVSVAKKYRGYGLPFEDLIQEGNIGLMKAVEKFDP
                     DRGFRFSTYATWWIRQAVQRAVADKGRTIRVPVHMTEKIRKVSRAMSELALEIEREPT
                     EEEVARRLDWDPDEVRLTMRAMPDATSLDQPVSSEETASQLGDFIEDDKVSDTPDTVM
                     REMETEHLKEAIEGLPERARYVLVRRYGLDDREPATLAELGDELDISRERVRQLQREA
                     ERILKGGEYGRVLRDAVA"
     gene            16344..17336
                     /locus_tag="VFQ10_00340"
     CDS             16344..17336
                     /locus_tag="VFQ10_00340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9963813.1"
                     /translation="MHGPRRLVALRCLATVAVILGFMENASRIPDRINEDIANIVACW
                     QARRRGDNEDGFYDRMLAGMEELVSVFVGFLRSTETVETFSRGGATRAMVHRIAQHQH
                     DLGRDAVGVIEDFSVLRRCIWRSVEAGVDLGGLHGEEVSGFFAKLMQASDWLTETGLE
                     AFDAIVRREMEQALGRAAATDLVTGLPDRDQLNRLLLPRAIDSYERFSVAVFDVADFT
                     ETVAAGKVKRARKVLRTLAEAVQASSPRGSVFARFGDDEVCVILPGETGEGGYRLAER
                     VLRRLAEGKADFEVDVGVAEYPAHGTNPGELMGETLKALKMAKRVGGSGIVVAR"
     gene            17354..17674
                     /locus_tag="VFQ10_00345"
     CDS             17354..17674
                     /locus_tag="VFQ10_00345"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9963814.1"
                     /translation="MIAIFNSLPVKEGAADEIVDRFAESRGHVQGFPGFLSMEVLKSD
                     AEDEVLVVTRWRDRESFEAWVHSDAFSRAHGLSGAGGLLTGHPRMTSYRVAVERAPEA
                     SGPG"
     gene            complement(17678..17815)
                     /locus_tag="VFQ10_00350"
     CDS             complement(17678..17815)
                     /locus_tag="VFQ10_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963815.1"
                     /translation="MRERLAFGILILLLICLFAFTFAVLGKGEYEPTGVGSPQISQSL
                     A"
     gene            18000..18695
                     /locus_tag="VFQ10_00355"
     CDS             18000..18695
                     /locus_tag="VFQ10_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9963816.1"
                     /translation="MAVDISLTEYTKLQEQECRSLLQILGEMGIATADRVYGPGDAIY
                     NQGEYGDALYVLTSGVLKLFRPYSGSKEATLRLLKPWDIFGHLAFAGETRQRAYAEAV
                     TECRVTKVPKIFVERAVRQEPRAAFKLMTLLELRLVQYEELVKCLLPRETEVRLANLL
                     PLLAQKFGQSTDGLVTIDLRLTHQDLAAMIASTRESVTKVLNEMRNRGVIEVDSGRIT
                     LKDTRTLAKLSRD"
     gene            18833..>19470
                     /locus_tag="VFQ10_00360"
     CDS             18833..>19470
                     /locus_tag="VFQ10_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET9963817.1"
                     /translation="MLELIEAVILGVVQGLTEFLPVSSSGHLLLGQYFLGLDQDRFGL
                     SFDVALHMGTLVAVVSYFWRDLIRMALAFLRSFGNRDLVNDPDQRLAYLVIASTIPAA
                     LIGYLFEGFFEDTIRSPWVVVFNFVLVGVLFLVAEAAASRSRRATKLGFGEAVGIGLA
                     QAAALVPGVSRSGATITLGLFLGLRREEAARFSFLMSVPIIAGAGGLQLAEV"
BASE COUNT         3219 a         6022 c         6191 g         4038 t
ORIGIN      
        1 cgtcggcgta gacgggacct atagctcctg tgtgtatgac cgcgttgctc catgcggcct
       61 catccagggt cgtcgagacg acgtacttgg gcacgccgtt caggaagtcc accatcggcg
      121 tgccgcccgg ttggttgggc cagtaggcgg ccattcgctc gtacgtgact cgccccatca
      181 gcatcgcgtc cgatgcggcc atttcagacg cgttcgcctc ctccatctcg gcgtttgagt
      241 aggaaaaagc ccactcttcc ggcgactcca tcacaccgtc cagcgacacc aactccacag
      301 gcgacgactt tcctcacgga tctgcttcgt gtatcattcc cgatcgtctg gccgtagcat
      361 aaggtgacat acgtgaaact caaccggcat acttcggaga actctcgcac cggcgggtcg
      421 gtgagtagcc gggggaagag tgctgctcgt aggtagaatc atatggtacg tagtttcggc
      481 gggtgggagc tccgcgaaga ggagacgcca tgacaaccat aaagcccgac ttgcctgtta
      541 tcccgttcgc ttcccgcggc gcgtgggagg cgtggctcaa agaacaccat gcgacctcgg
      601 atggactgtg gctcaagatc accaggaagg gctcagatct agagagggtc tctgtgaaga
      661 gacccttgag gtcgccctgt gctacggttg gatcgacagc caggcgggcc gcttcgacga
      721 ccagtattgg ttgctccggt tcaccccgcg ccggccgaag agcaagtggt cgaagggtaa
      781 ccgcggcaag gtgaggaagc tgatcgagga gggcaggatg aaacccgccg gcttgcggga
      841 ggtggagcgt gccaaagccg agggccgctg ggagcagtag tctagttgca gcggcgaata
      901 ctataaccga agaacgacgc ctcgaacggc acattgggac gatcgcccgg tggagcaccg
      961 ggtccgagca catggtcgcc ttcacgggcg ctggcatcag caccgactcg ggtatccccg
     1021 actttcgcgg ccctgagggg gtgtggacac ggcgcgatgc cggcctgccg gcaccaaggt
     1081 ggcgggttcc gccgggtgag gtagcgccca acgcatccca cctgtctctc ttcgaactcc
     1141 agcggctcgg caagctccgg ttcctcatca cgcagaacac cgacaacctc caccgccgct
     1201 ccggcctcaa ccccgagttc ttggccgagt tacacggtaa tgggcagctc ttacgctgcc
     1261 tcgactgcta ccgactctac accaggcagg aggtagggtg ggacaggggt cggtggggcc
     1321 cggcctaccg cacccagaag ccggtgccag gccagccgga gtgcgccgac tgcggtgggc
     1381 ggctcatctc ctcggtggtg aacttcggag atccgttgcc ggagacagag ttgatgctgg
     1441 ccgaccacca cgcccgccgc tgcgacctga tgctcgtcct cggctcctcg ctcatggtgc
     1501 agccggcggc ctcgctcgta ggcctggcac tcagatccgg ggcccgggtg gttctggtca
     1561 accggggcaa gacaccctac gatagggcgg cgacgctgcg ggtgtggagg ggtatcggtg
     1621 aggtgatccc gcctgcggtc gagcgggtga gggaggcaat cggggatcag aaagagtaga
     1681 gacgagatcc tgtaagcact ccttctcgta acgtttgtcg taacgcttga gagacgatcg
     1741 tactgtagag tagcgttgtg acagaagggg caatagaaca gggtcgaagt gtcgacggtg
     1801 caagccagcg ccttgcctac tggctcgctt gagctacatg tgcgctctcc ttgacgctca
     1861 cagccctcaa cttccttctc ttaactgaat gtgaggttga atacccccgc ctacgtcttc
     1921 tggcccgagt tgacctccct cgcggtgggt tactcggtga tcggcgcaat tatcgcctcg
     1981 cgcctgccca accatccgat cggctggatc tgttgtgcaa ttggacttat tgtggcggtg
     2041 gaccacttcg ctggcgagta cgccgtgtac gccctactgg cgcatccgca cgggcttttg
     2101 ggcggtaggg cgatgctttg gctccagggt tggttctgga tgctattcat cggcctcatc
     2161 gtgttcttgc tcctactgtt cccaactggc cggttgccca acaggcgctg gcgcactttc
     2221 acgtgggtga gtgtggccgt gatttcagcg gcgatgatat ggtcgtcggc catctcgccg
     2281 gatgtcggct tcaatgcgcc tccctccccg gttcaactct ctgtacttct tctcggagga
     2341 gtcgcggcag gctccgtgat tgtggggcgg cgcaatgcga gggggataga gctccagcag
     2401 atcaagtggc tgctctatgt tgggccgttg ttctttatcg ccgctggcct ccaaatcggc
     2461 ttctactact tttggttggc agagaggtcg tgggggttgt gggctagtta tttgctcgtg
     2521 gcaatcggtg gcttgggcgg ccccatagcc ataggtatag cgatcttgcg ctaccggctc
     2581 tacgagatag acatcctcat aaaccgtacc ttcgtctacg gctcactcac ggctacgctg
     2641 atcgtcctct acttcggtgg catcgtagtg ttgcagagag tcttcgtctt gcttacaggc
     2701 cagcagtcca ccctcgcggt cgtggcctcc actctagcca tcgccgcttt gttcagccct
     2761 ctgcgccgcc gtatccaagg gttcgtcgat cggcgcttct accgtaggaa gtacgatgcg
     2821 aggaagactc tggaggcctt ttccgcgaag ttgacgagcg agacggacct cgatgccttg
     2881 accgacgatc tcatagaggt ggtcagggag acgatacaac cggcgcacgt gagtttgtgg
     2941 ctgcgtcccg agagggcttc taaaggccgg cagacggact agcgacactt gttcacccga
     3001 gtgcgtggac atggcctctt tttccgaagt ggaggcgggg aatcgacccg agcccgcgat
     3061 cgggtcggct ccaggttcag gcgagaggct ccgagggctg atgtaccggc ttatcctgcg
     3121 gcggtgccat agtcgtaggt gggcaggggg ccgccggcgt ccctgattat gtagccttcg
     3181 aacatccgct tgactacgtt gggctcgacc cccgctatgt cgttgtgcat accagggtcc
     3241 tgctccacgt catagagttt ggcgtcggcg ccctggttgg tgctgaacat gacgtagcgg
     3301 tcgtcgcggg cccagacatg gtcgttgtag ccgatcgtaa agtggggtct cgcctcgggc
     3361 ttcccgcctc cgagcacgac ggagaggtcc tggccgtcca gttcctgcct aggttcgatc
     3421 ccgaggaacc cgagtatcgt tggcgcgacg tcgtgggtgg aggcgtagta gccgctcgtc
     3481 tgtccagccc ccttgccttc agggtgcctt atgtagaacg gaatgtcggt aacctcgggc
     3541 cagagcacgt tgtccggctt gcccgtgtag ccgtgctcgc cgtgggcgac gccgtggtcg
     3601 gagaggagta tgaggagcgt gttatcgaag aggcctagct cttccatttt gtcgaggaag
     3661 cgccccaacc agcggtccgt catggtgacc tcagccgagt agcgcgcctt cattctctcg
     3721 agctccctcg gcagcaggta gctgctcgga ccgtagatca ccgagaacgg ctccttgagg
     3781 ttgtagggct cgtcgtcgta catctcgacg tacctctcgg gcgggtccca gggctcgtgc
     3841 ggatcgtagc tgtccacggt aaggaagaaa ggcccaccct tgctcaagac ttcgaggtag
     3901 tccgcggcct gggagaagac catgggtgag aaccagtcgg cctccgtctt acgctccttc
     3961 acgttggcga agtactggcg catctggccc gtcatggctg ggatgttgcc cttgagaagg
     4021 gcctgggcga ccttgtcttt cgggtatgtc cacgccggcc tgtaattgtc agtggtctgg
     4081 ccgcggaaga agtcgaagac gtcgaagcca cgatgaatgt tgtaagaggg cttgaactgg
     4141 tgcatgttgt ccgtcacgaa gtaggttccg taccccgccg cctgcatcat ctcggcgagc
     4201 gtcgtctggt agttgggtat cggttcccag ccctgcagga ttatgtcctc acccttgggc
     4261 ggtacccagt ctctgaaagg ccaagtcctg aggcccgtgt gtatggccct cctagcacag
     4321 atcgtcggta tggactcagg gtatgccttg gtaaaaagca agctatcttt ggcgagggca
     4381 tcaaggttgg ggctccggat cttatcgttg ccgtagacgc cgatgtgatc cttcctcagg
     4441 ctgtcgatga tgataagcac cacgttagta tcctctttga cgccgggggg atcgagtgtg
     4501 atgttgcgga tgcgctctgg gaggtcgcag cctgtggtac cgatcagcgt cgccccggcc
     4561 atcccggcgc cggtcatctt gaggaagtct ctgcgggtca gcgtgcgttt tctcacgaag
     4621 caagagcgta ctacgcaaca atgtgaccta caagccgaac gggcgggccg tcaccgtgaa
     4681 ccgcgccacc ctggcgagct gggggactgc tagaatgatc tctatgagaa gcatcatggc
     4741 cgtcgggatc gggttgctgc tggcgctagt gctcggtcta ctcgtggtgc aggggatatt
     4801 cgcgccggtc ctcacacgtt tcttcgggct cgagcgcgca ggccccgccg ccctcccgct
     4861 catcttgctc gtcttcgccg ccgcgttctc gttctacttc ggggggatgg ccgccagcta
     4921 caaggcgccc tttctgcatc ggctgcacgg ggttctggtc gtccctatag ccttcgttct
     4981 ttcgttggtg ctcaacttcg tgctgggtaa agggtttctg ccaggcgtcg atggtacctg
     5041 ggccgcgggg ctcgtggccg gcttcctcgt cgtctcgggc acggcgtcct acgtaggggc
     5101 cctccgtggg gaggcgctct atgcccacaa ccagaaggtc gcgcggcgcc accgttagcc
     5161 ccggctagcg ggcctggacg ccgatcatca aacgccccct ggagtccatc ctagcgttgg
     5221 gctgccggct cgacgcctcg acttcggccg ggtcttcacc cgtacccgtg aggtagaccc
     5281 tgggttcctc gaccccatct gtcgggtcgg agacgtagcg gcgccaggcc atgtcgcccc
     5341 agaggaacgt tagcgttggt ctccctgggg ccgtgtcgtc gagggctgcg tggaccctag
     5401 ggaggccgaa ggccttcgag atggacgcta cggtcctcgc gtgctcgctc tcgttgaaca
     5461 gcgctatgcc ctccctgagc cggcgtgccg ggtcggagag ccggtcgagg ctttccaggg
     5521 ctctttgtag ctcttcggcg ttgcgctggc tctcttctct tgcctcagaa agtctggctt
     5581 ccaggtcttc gaccttctcc tcggcaagta tgttctgcgc ctcgatatcc tccagaggcc
     5641 ctttcggttc tccgtgtgcg gcattgtcgg gcctggctgt gatcccggat gactcctcac
     5701 tgctggcgcg gatctcgccg agtacggtct ccagctcgtt cctgcgagcc tcggactctt
     5761 ccagggcccc ttccagctcg gtgacgcggt tctcgaagtc ggagatctcc gccccgaaac
     5821 gctctaccac ccgctccaga tcctcttgtg aggtggactt ctgggccagc ctggctgcct
     5881 cgagctccgt caggcgggcc gtgtaggcgc gcatttcggt ctcacgctgg accgcggctt
     5941 ccctcaaccg ctcgtcgagt gcccatgtct ccgccttcct gcgttcgtcc tcggccacga
     6001 gttgctcctc gtgctcgcgc ctgaggcgcg ccgtctcctg ctcgtggcgc gccctgagcg
     6061 ccctgttgtc agcctctctc tgggcccgga tcgccgcgac cctcttatca gccgactctt
     6121 tcgcctcggc gagcctggtc gcgtgcgtct ttctcaggtc gttctcgcgc gcctcagcga
     6181 gcgccttgaa ctgccgcagc tcctctttgc gccgttcctc cgagcttcgg ctggccgctt
     6241 cgagctcggc gcgcagcgac tctatctcct cttgctgcgt tcctaccacg ttctggagct
     6301 ccaactccct ctctgccgct gcgctcctca aggcctgcac ggccgccgtc tgacgatcgt
     6361 cggcagcgct ccgctcctcg gccagcctcc tttcgaagtc ttctctcaca gagtgctcga
     6421 gggcattctc cctttcggcg gccgcgctgc gggcggattc gagctcggcc gcgtggcgcg
     6481 cctcctgctc ctggtaagac gtccttaact gctcctccca atgctcggag gcgttcttca
     6541 gggcttcgat ctcctccctg tatcgcgcct ccagggcttc gcgttcgcgc ctgccctctt
     6601 ccttgatggt gtcgaggcgg gtctcggagg aggccgcatc cacgtcggtg cgtcgttgct
     6661 cttcgagcct gttgcgcacc tctgcgagct cgtttatgtg ctcgttgctg aggcggagct
     6721 gctcgtcgga gtacctctgt ttgaggttgg agatctccgc cctgaggtcc tcggcctcac
     6781 tttcggcctc gacgcgggct ctctctttct cctccagcgc gccggcccgg aaggagacct
     6841 cctcctgcgc agccttcagc gctgaggccg cctggtccag gtcgctcctt agggcagcca
     6901 cctcctgctc caggttctcg atcagggcgc tggctgcctc cattccctca gcgagcgcgg
     6961 ccgccttatc cctaggatcc tcgctcatga tcgcctctcc tatgttaccc gcaacaccac
     7021 attggtcgaa agggtaactc ctggcaggcc tgcagtaaag tccctgctta ctcgctgtca
     7081 ctgcaggcag ggcacaggcc ctcgaggacc atcgtcgcgc gggtgaccct gtaaggagta
     7141 ttgttgcgga tgatctcttc cacgccgcca agtgcgacac cggggacctc ggagacctct
     7201 ccgcatctga tgcacctgat gtccaggtgc ggctccacgt tgaggtcgta gcgcgttggg
     7261 gactcggccc aatgcttgga gcctataacg ccaatctctt ccagtaccga gagcgcctgg
     7321 tagaccgtgc ccagcgccac gcgcgggttc tcgcgcttga cgatcaggta gacgtcttcg
     7381 gctgaagggt gtcccttcgc tcctaatagg gcctcgagaa cggccctgcg ctgggaggtg
     7441 agggtgtagc cagacctgcg tagcttctcc ctgacgtcgg gtacgagcac gcctgacgta
     7501 tcctcctgcg ctgtctggtg atcagtaacc attcctgttt ccgagtttac catacggcgt
     7561 ttttcgtgct gcggtgtacc atagctgcca gatgtcgttg tttgccgacg ttctggtgaa
     7621 cctctggcgc cttatccgta acgcccgcgc gcgtctactc ggcaggccgc cggagtacgt
     7681 gtggatcgag gtcggtggcc ctctggccga gttcgagacg cctgttggcc tcctccggcg
     7741 ccgcttgtct cggggaccga gcccaccgac gctggagagg ctgcgcctgt ggctcgacag
     7801 gatctccgcc gacgggcgtc cgcgcggggt cgtgctgcgc atcaagaaac tcgatgccgg
     7861 gtgggccgcc atcgaagagt tgcgtcgcga gatcctcgcg ttccggtcac gcggcggccg
     7921 ggtcgtggcc tatttcgcgg attccgcgga catccgctcc tactacctgg cgtgcgcggc
     7981 ggacgagatc ctggctaccc cgctcgccga cctgaacgtc gttggtatgc gcgccagggt
     8041 agatttcctc aaagacgccc tccacaacct cggcctcgag gtcgaggtcg tcgccgtctc
     8101 gccttacaag tcagcgggtg aacggttcgt cagcaacgac ttctcacgcg agtcccgcga
     8161 gcaggctggg cggctactcg atagccgctt cgaggaggtg gtcggtgcga tttccgaagg
     8221 cagaggtctc tctcgcgagg aggttcgcgc aaagatagac ttggccccgt atggcgcgcc
     8281 ggaggccctc tcggaagacc tgctcgacgg tgtcctctac gaggacgagt tgccgacgag
     8341 gttcgggtcg gaggaacggc acgccagatt agcggagtgg ggcctggcgc agggatccct
     8401 gcgcatgcct tacaggaggc ggtcgcgaaa aaaggtcgcg ctcgtcaccc tctcaggggc
     8461 tatagtgcgg ggccgaagta ggagactgcc cttgccgcta ccgttcgtcg ggggggagca
     8521 ggcggggagc gagtccgtag tcgccgcgct gcgggtggcg gagaaggccc ggagggtcgc
     8581 tgccgtactg ttctacgtcg actcgcccgg cggcgacgcg ctggcttcgg atctgatctg
     8641 gcgtgaggtc gaacgcctca gtgccaaaaa accggtcgtc gttcttatgg gaaacgcggc
     8701 cgcctcggga ggctactacg tctcggcagc ggcgagccac atcgtcgcac gccgcggcac
     8761 gctcacgggt tctataggcg tgctggccat ccgtcccgtc gcgctcggac tctacgagaa
     8821 gctgggtatc agccccgtcg ccctggaaag gggggcccgt gccggcctcc tcgatcccag
     8881 ccgccggccc agtcctgacg aaatgcgggt gatagagggc cagatcggac gcatctacac
     8941 cgagttcaag gaccgtgtcg ccagggggcg cgggaacgat cttacggacc tcgaaggcat
     9001 ggcaggcgga agggtgtgga cgggtatcga ggcgttggaa ctcgggctgg tcgacgagac
     9061 tggtggtttc agagatgcgc taagcaaggc cagggagctc ggtggcattg atcgggatgt
     9121 accaggggtg ctgacgaagg tcaatccgcc ccgaggcgta cggcctcttc ccccagagcc
     9181 cgcgcagacc cttttggacg cgctcgggga gatgtggctg gcgctatctg agcttcgcgc
     9241 cggcggcgtt ttggcgctcg cgccctacga aatttcagaa ggctgatagg attttcggcg
     9301 actgccgcta cttcagaagg atcctgtatt gagcgagatc cccaacaaag gcgttgaccg
     9361 gcgggcgatg gcggtgctgt cggctggcca cctgttcacg gacctcaatc agggagccgt
     9421 agctgctttg ctgccttttt tgatcgcgga ccggggtatc tccctggccg cggccggggc
     9481 cattttgttc gccgctacgg tgagctcgtc gatcgtgcag cccttgtttg gcatcttctc
     9541 ggacagaagg ccggtgcccg ccctcatgcc tctcggtgtc ttgctcgccg ggtgcggaat
     9601 ggcgcttgtt ggcctggcgc cgagctatcc cctgatcctg ctttgtgtag ttctgagcgg
     9661 tatcggtgtg gccgcgttcc accccgaggc ggcgaggttc gccaactacg tatccggatc
     9721 gggccgggca cgcgggatga gcttcttctc cgtgggcggt aacgccggct tcgccctcgg
     9781 acccgcactg gccacgccgc tcgtcattct cttcgggttg ccaggagccg tgttcctcgc
     9841 agtgccagcc gtcattatgg ccgccgtgct attccacgag ttgccacgga tgctggggtt
     9901 cagacccgag gcggtggaga gcgaaaggga gacggtggcc aatgcgcccg aacactgggg
     9961 ccccttcgtg cttatgatcg gggtggtcac agtgcggtct ttcgtgtact tcgggctcgt
    10021 tgccttcgtc gcctcctatt acgagcgcgt gctcgatgtg tccccggcgc tcgggaacac
    10081 ggcactcacg ctgatgcttt tcggcggagc tgcaggtacc ctgacaatgg gtcccctcgc
    10141 cgacaggttc ggcagacgta cggtactcgt ggtctcgatg ttggtgctgc cacctctggt
    10201 cttctgcttc acgctcgtcg gtcccttctt cggcatggcg gtgctcatcc tagccggggc
    10261 ggctacggtg gggacgttcg ggataaccgt cgttatggca caggagtacc tgcccggacg
    10321 catcggcctc gcggccggtt taacgatggg gctctctatc gggctgggcg gtgtcggggc
    10381 gccgctgctc ggcctcgtcg cggataacgc gggcctctcc ctgacgatgc tcgttatagc
    10441 cgtattgccc ttgccagggc ttatcctcgc cctgaccctg ccccgcggga agtaggggcg
    10501 gttcgcgaac ctcacctact agctcactgg ttcgtgttgg agaacaccat tccttctcct
    10561 cgggtcgagt accatagcgc cggtgagcgc ggcgtttaga gagggctacc agccagggtg
    10621 agcgtattcc atttcatcgg cgtcttcgcc taccgcttcc ggtgggcaat cttgatgttc
    10681 tggggcatgg tcctgatctc gagctccttc ttcgccccgg ggctctcggg acaactcaag
    10741 ggcggtgggt tcgaaggcgc gaactcggag gccgagaggg tgcaggacct aatgtcgggc
    10801 gagttcggcc tctcgccagc gaccctgacg gtggtcttta ccggcgacgg tatccccgcc
    10861 cgcagcgaag agttccagag ggcccaggag gatgcgctcg cgggcgtacg caagctggac
    10921 gacgtacgcc aggtcatctc atacgccggc tccaaagatt ctcgctttat ctcagaggat
    10981 ggggagaagt cctacgcggt ggtcacttcc gatgtctcta tcgacgcgac tcgcaacgtg
    11041 gttgacgagg ttaggagcaa ggtccgctcc gacaagctcg atacctacgt aacaggggcg
    11101 ccggccgtct atcaggacct cgaggaggct agcaacgagg acgtgaagga tgctgagaaa
    11161 tacgccttcc ccttcgccgt gatcatactc gtcttcgcct tcggcacgct cgtcgccgcc
    11221 ggtgtgccgg ttttgatcgg gggcgcgagc gtggtgacgg cgctcgccgc actgtacttc
    11281 attgcgggca tctacgacat gtccgtgttc acgcttacgc tctcgacgat gctcggtctc
    11341 gggctcggca tagactacgc gctgttcttc gttagccgtt tcagggagga actcgaggac
    11401 tacccgaccg tggaggccgt gccacgcacg gtggcgaccg caggacgatc catcttcttc
    11461 tcggggacgg cggtgttgat cgggctctcc ggcctgcttt tcttcccctt tatgttcatg
    11521 cggtcgatcg gggtggccgg cgttgtcgtt gtcttcgtgt ccgttctggc ggcgctcaca
    11581 cttctccctg ccctccttgg tgttctcgga ccgaggatca acaggctcgc catcaggcac
    11641 cgcagtgatg gggcgggttc ggcgttctgg agccgcagcg ccgaggtcgt gatgcgacat
    11701 cctctcaccg tgatcctgct cgcggccgcg atccttggga ccctgctcta cccggtcggc
    11761 cacatgaggg tcggtatccc cgaggcgacc gtcttgccgc aggagtacga gtcgcgcgcc
    11821 ggagacgaca tcctgaagcg gaacttcgag tacgccgcac tcaacccgat ggagatcgtc
    11881 gccaccttcg tggatgaccc actcagcgtc cgcggtctct ccgacacgag agagctcggc
    11941 gagagcatca gcggcgccga cggtgtctcc cgcgtcgaga gcgcctacac gatagcagca
    12001 gcggctgcgg aggactacgc ggggcgcgtc gccgacgccc gcaatcaggc gaggaaggag
    12061 gccgcagcaa gggtggacgg gctcgtgcag cggcagatcg aagagcagac gctgaacgag
    12121 acggacagag cagtgaacga ggaactctca cagatagagg ctacctatgg cgccgtccct
    12181 cccgggaccg aggagcggct gcgggcagag atccggcctc gggtggagcg cgaggtgcgc
    12241 tcctcggagg gcaggataag ggatgaggtc gagcgccgcc tggccaaaga ggtagaccgc
    12301 aaggtgccgg ggttgcccga agggatctcg gccgacggta aggttacgcc agagggtgtt
    12361 gcgaacttcc tgaagttgcc agcggccagg cagtccgagg acctgaagga cacccttgac
    12421 acatacgtgg cgggagatag aacgctgctg cggggggtga ccgaggcgaa cccctacacg
    12481 caggacgcct acggcgcggt cgatgcagtg cgcgcagtcg aggcgccgga tggaatgagt
    12541 ttcctcgttg gcggactctc cgccggccag aaggacttca tatctagcct gtacagtaag
    12601 gcgcccctcg cggccgcctt cgttatcggg gtgacgtacc tgatcctgct catcaccttc
    12661 cgctccattt tcatcccgct gaaggctgtc atcgtcaaca tcctgagcct cacggcgagt
    12721 ttcggagcta tggtcttcgt cttccaggac ggccatctct ccggcctcct cgacttcacg
    12781 cctctaggct tcgtggacgc gacgctcccc attctgatgt tttgtacgat cttcggcgtc
    12841 tcgatggact atgaggtatt tctcctctcc cgcatcaggg aggcctacga gaacggcgac
    12901 tccaacaccg ccagcgtcgc aaagggtctg gtgtccaccg ccggtatcat cacctcggcc
    12961 gccgccatca tcatagtggt tactggggct ttcgctctaa cgggtatcgt cctgaccaaa
    13021 gcgatcggcc tcggcctcgc cgtggccgtg ttcgtagacg cgacgatcat ccgtatcctc
    13081 ctcgtccccg ctaccatgcg cattttgggt gattggaact ggtggccagg aggacgcaag
    13141 gttaccttcg gggcccgccc gaatgagccc gctagggact gaccgccgca tagaggaatc
    13201 tccgaaagtc ttcgaacgcg acgagtcccc gagataagat cgattcttga tcttgctgta
    13261 tgacggggat agactgtggt tgatgttgga tttcgagcgg atactcaagg acagaggcta
    13321 caggctaacc aaccagcggc ggatcatcgt gcatgagctg gagggggagc ggcatctttc
    13381 ggcggaggag atctacgacc gggtcaaggg cgagcatccc gagcttggcc tctccacggt
    13441 gtaccggacg ctcgacctct tgaccgagct cggcatcgtg cgcaaagatg acttcggaga
    13501 gggttactcg cgttacgagc tggcgacgga gcggatgcac caccacgcgc gctgcgagca
    13561 gtgcggcaag gtcatcgagt tcaacgagga gctgatggag tatctggcct tgcaggtcga
    13621 gcgggagacc ggtttcgtca ctgactggca cgagatcacg ctgtacggcc gctgcgccga
    13681 ttgcgcggtc tcttagacct cgaacgccag accgtcctcg gcaacgagta tctcgccttc
    13741 gaatcccctt cccacttcgg taacgaggtt cagctcgtcc gccgccgggt agatgtgggt
    13801 gaggacgaca cgtcgtacgc gtgcctcgct cgcgatgcgt gctacgcctt caggcgtcag
    13861 gtggccggga acggggactt cggcggggaa ggaacactcg atcaggagtg tatcggcgcc
    13921 gcgcgccagt tcgaccaagg accggctgta ctcggtgtcg cccgtgtata cgagcgcgcc
    13981 gccctcggca tccatcctgt aggcgatgct ctcgctgcgg tgctccgccg gggcccacga
    14041 gagccggcat ccggccaggt cgatggggga tcggcattca tggggtagtt ccgagactag
    14101 cgtagcgtag tcgccggcca tccactctcc ccagaccttc atcacagagc tccagaactg
    14161 gaggaaaggc tccggaccgg ttacgtagag aggcagggtg cgcttttcat gtgcgccgta
    14221 gttgatggcg aagaggagag gaacgacgtc taccgtatgg tctgggtgga agtgcgtaat
    14281 gaagatgcgg tctacggaga ggggatccag gcccgatcgg agcatgccgc ggagcgcacc
    14341 cgaaccgagg tcgaagacga gcatctgccc accggcgcgc accacgacgc agctctggcg
    14401 cctctccagg cgtggtacta cggtgcctga tcctacgacg gtgatcttca ggggcactcc
    14461 tttcagtcgc aattttagtt cgtcaggtct tctctctgtt ctggcatgtc gagtgcttca
    14521 ttgctggctg ctcttcgaac gcccatgcga gtccggccat gccgagcagg gcgccgccga
    14581 tgacgtccga gggccagtgc gttccgaggt agaccctact cgtggctgaa gcgacgagga
    14641 agatcaagag cgccaccctg ccaggtctgc tcggccacag taagtagatg gcgccgagaa
    14701 ggaggactga acgcagcatg tgcccgctgg ggtaagggta tggcgtactc acgtcgagta
    14761 acgatgggtc cggcgcccgc atggcgtccc cggggacggg tgtctgcggc accaccgttt
    14821 ttgctgcgag ctcgatcagt cccgtaaaca cgaaggcgac gacgagacgt agcgcgaggc
    14881 ggccccgttc ttcgaccagc aaaccggctg cgagcgcgac cgtggcgacg gcgacgaact
    14941 cgactccgcc taccacggag gtcaccatcc cgaccccatc gaggatccct gagtctgtcc
    15001 tctgggccag gtcgagtatc aggacatcga ggggacgaaa tagccctgcg ccggcggccg
    15061 ccgagcaaac gaggaatgct gctgtcgaga gcagcaggat cagcctgttg tcgcccttca
    15121 cgtccttaca ggttatcaaa ggggacatga aagcgccggg ccccacggcc cggcgatctt
    15181 ggatccgcga tgtcgaagaa aaaagcgatt cgcagaggtg ccaacccacc gcgaatcgcg
    15241 cattcaatta ccagcgtttg caatgaggtt taacctcatt gcaaacccct tctatgctac
    15301 agcatcgcgc aggacgcggc cgtactcccc gcccttcagg atacgttccg cctccctctg
    15361 cagctgcctg acccgctcgc gcgagatgtc aagctcgtcg ccgagttcgg cgagggtggc
    15421 cggctcgcgg tcgtcgagac cgtagcgcct gacgagcacg tagcgcgccc tctcgggaag
    15481 accttcgatc gcttccttca gatgctccgt ctccatctca cgcatcacgg tatcaggcgt
    15541 atccgagacc ttgtcgtcct cgatgaagtc acccagctgc gaagcggtct cctcgctgga
    15601 cacaggctga tctaggctcg tggcgtccgg catggcgcgc atcgtgagac gcacctcgtc
    15661 ggggtcccag tccaggcgcc tggctacctc ttcctcggtg ggctcgcgtt cgatctccag
    15721 ggccagctcg ctcatggccc ggctgacctt cctgatcttc tccgtcatgt gcacggggac
    15781 gcggatggta cggcccttgt cagccaccgc gcgctgcacg gcctgacgga tccaccacgt
    15841 ggcgtacgtg gagaacctga aacccctatc agggtcaaac ttctcgacgg ccttcatcag
    15901 gccgatgttg ccctcctgga tcaggtcctc gaagggaagc ccgtagcccc tgtacttctt
    15961 ggcaacgctg accaccagcc tgaggttctt ctcgatgagc ctctgcctgg cccgcctgtc
    16021 gccggccttg gccctcacgc tgagatctat ctcctccctg tgcgtcagca ggttgccctg
    16081 ccctatgtgc gcgagatact tggcgaggag ctcaggggtc tccgattcac gctcgatctt
    16141 ctgattgtcc ttacgctttt cgaaattggc tttcaaaatg ccctcctcac tacacaccca
    16201 tcttaccgga aatcagtttt agaaactact attagtttta ccgcgaggtt taatttccta
    16261 aacctctccg agggctagct cttcaccggg tctcatcacc acttgtcact gcacagatac
    16321 atacgtatgt cgacgcgaaa tagttgcatg ggccccgccg cctcgtcgcg ttgcggtgcc
    16381 ttgcaacggt cgcggttata cttgggttca tggagaatgc ctcccgcatc ccggaccgca
    16441 taaacgagga catcgccaat attgtggcct gctggcaggc gcgcaggcgt ggagacaacg
    16501 aggatgggtt ctatgatcgc atgctcgccg gtatggagga gttggtctcg gtgttcgtcg
    16561 gctttttgcg cagtacggag acggtcgaga ccttttcccg gggtggcgcg accagggcta
    16621 tggtccacag gatcgcccag caccagcacg atctgggcag ggatgcggtc ggggtcatag
    16681 aggatttcag cgtcctgcgg cggtgcatct ggcgctccgt cgaggccggt gtcgacctcg
    16741 gcgggctcca tggggaagag gtctcagggt ttttcgccaa gctgatgcag gcttcggact
    16801 ggctgaccga gaccggactg gaggccttcg atgccatagt gcggcgcgag atggagcagg
    16861 ctctcgggcg ggcagccgcc accgacctgg tgaccgggct tcctgacaga gatcagctca
    16921 accggctact tctgccacgg gcgatcgatt cgtacgagcg gttctccgtg gctgtcttcg
    16981 acgtcgccga ctttaccgag accgtggcgg cagggaaggt caagcgcgcc aggaaggtgc
    17041 tgcgcacgct cgccgaagcc gtgcaggcgt cctcgccgcg ggggtctgtc ttcgcccgct
    17101 tcggggacga cgaggtctgc gtgatcctgc ccggcgagac cggtgaaggt ggctaccggt
    17161 tggcggagcg ggtgctcaga cgtctggcag agggcaaggc agacttcgaa gtagacgtcg
    17221 gtgtcgccga gtatcctgca cacggaacga acccggggga gttgatgggg gagaccctca
    17281 aggccctcaa gatggccaaa cgcgtcggcg gcagcggcat cgtggtagcc cgctaggtag
    17341 tccggaggta accgtgattg cgatcttcaa cagcctgccc gtcaaagaag gagccgccga
    17401 tgagatcgtg gaccgcttcg ccgaaagccg cggacacgta caggggttcc cagggttcct
    17461 ctccatggag gtcctcaagt ccgacgctga ggacgaggtg ctcgtcgtta cgcgctggcg
    17521 ggatagggaa tccttcgagg cgtgggttca cagcgatgca ttctccagag cacacgggct
    17581 cagtggagcg ggcggactac tgacgggcca ccccaggatg acttcctaca gggtcgcggt
    17641 cgagcgtgct cctgaggcct ccggccctgg ttgaggatca ggccaggctt tgcgagatct
    17701 ggggcgaccc caccccggtc ggttcgtact caccctttcc gaggacggcg aaggtgaagg
    17761 cgaacaggca gatcagtagc aggatcagga tcccgaaggc cagccgttcc cgcaccttgt
    17821 ctcctctctc gaatacgaac ctccgcgcca ttctaaccga atcagcaagg cacgtcctgc
    17881 gcaatgccct cagctcagcc cgcgcgtaat gtacaatgta gcaatgtgta gcacgctcag
    17941 tcccgaggtt cgtgagtatc ggtgtggtct cggaacggaa tgccagggag gggacccacg
    18001 tggccgttga tatttccctg accgagtaca cgaagctgca ggagcaggag tgccggtcgc
    18061 tcttgcagat tctcggtgag atgggcatcg ccacggccga cagggtctac ggaccagggg
    18121 acgccatcta caaccagggc gagtacggtg acgccctcta tgtgctgacc tcgggcgtgc
    18181 tgaagctatt caggccctac tcgggcagca aggaagcgac gttacgcttg ctcaagccct
    18241 gggatatctt cggtcatctg gccttcgccg gggagaccag gcaacgcgct tacgctgagg
    18301 ccgttacgga gtgcagggtg acaaaggtac ccaagatctt cgtcgaacgc gccgtccgcc
    18361 aggaaccgcg ggccgccttc aaactgatga cccttctgga gttgcgtctc gtacagtacg
    18421 aggagcttgt caagtgtctg ttgccccgcg agacagaggt gcggctcgca aacctgctgc
    18481 cactattggc gcagaagttt ggccagagca ccgacggtct cgtaaccata gaccttcgcc
    18541 taacccacca ggacctcgcc gccatgatcg cctccaccag ggaatcggtc accaaggtcc
    18601 tcaacgagat gcgtaaccgc ggcgtgatcg aggtcgattc cggccgtatc accctcaaag
    18661 atacccgcac cctcgcgaaa ctcagccgcg attagcttct cagcatgtca gcacggcggt
    18721 cgcgctgcct tgtcaaccag gacttgcaca ctacaattct gaattgctga caagcgcccg
    18781 agagggcacg ttctgaaatg ccgactagtt gaaaagctga attggggggc gtttgctcga
    18841 gcttatcgaa gctgtgatcc tcggggtggt gcagggtctc acggagttcc tgcccgtctc
    18901 cagctcaggg caccttctgc tcgggcagta tttcctcggg ctcgaccagg accgattcgg
    18961 gctctccttc gacgtggcgt tgcacatggg caccctcgtc gccgtagtct cctatttctg
    19021 gcgggatctc atcaggatgg ccctcgcttt cttacggtct ttcgggaaca gggatctcgt
    19081 caacgatccc gaccagcggc tcgcttacct cgtgatcgca tctaccatac ctgcggcctt
    19141 gatcgggtat ctgttcgagg gtttcttcga agatacgatc cgctcgcctt gggtggtggt
    19201 cttcaacttc gttctcgtcg gcgtactgtt cctagtagcc gaagccgcag ccagccgcag
    19261 ccggcgggcc accaagctcg ggttcggcga ggccgtcggg atcggactag cccaggcggc
    19321 cgcgctcgtg cccggcgtct cgcgttcggg ggccacgatc acactcggcc tctttctggg
    19381 gctcaggcgc gaggaggcgg ccaggttctc gttcctgatg agcgttccta tcatcgccgg
    19441 cgccggaggc ctgcagcttg cggaggtgat
//
LOCUS       DASTKM010000010         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_706314, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000010 DASTKM010000000
VERSION     DASTKM010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706314"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /gene="rocF"
                     /locus_tag="VFQ10_00365"
     CDS             complement(<1..883)
                     /gene="rocF"
                     /locus_tag="VFQ10_00365"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HET9963818.1"
                     /translation="MFESGRGPVGIFGVPMDLGQDRRGVDMGPSAIRYARIEACLEEL
                     GHRVTDLGNAGVPIPETVVAGAEVKHLAAVKSVCEEVSERAAAIVSEGMFPIFLGGDH
                     SIAIGTVSGIARSASGERTGVIWVDAHADFNTPATSPSGNIHGMPLATLTGRGHPDLV
                     GIGSPGASVRTEDVVIIGLRSVDVEEKRLLREAGVRVYTMKDIDAYGAARVVRSAIKG
                     LTDVDRVHLSLDLDAVDPEVAPGVGTPVRGGLTYREAHLLMELVNEAGIISSLDVVEV
                     NPILDVKNGTATIAVELV"
     gene            complement(876..2426)
                     /locus_tag="VFQ10_00370"
     CDS             complement(876..2426)
                     /locus_tag="VFQ10_00370"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9963819.1"
                     /translation="METIGPNSTFAAAAGTPYAAEAAAETYLAGGNAADAGVAAAAAV
                     SVTEPLMSSIGGGGFALVRDPAGGAELIDYYDAMPGKGLPDTAFGAGGSPQTIILKYG
                     AGVRSIVGGASSAVPGSLRGWEKLLARHGRLGMKEALAPAVRFAREGFRLCKTSALWF
                     GVAEEVLRLTEETRKHFYKGERVYLEGEEVRFPELADTLEAIGEGGADLFYEGGLGRT
                     ISSYMLKMGGIITEEDLAEYEAVIREPMSVRYGAGAMHTNGPPSAGGPTLAQMLKVVS
                     AYDLASLPEAEYVRVMAGAMKLALEDRETAYLDGSENRRVAERLTGEEYAAEQRRRIF
                     STFGSPHTTHLACVDSEGLAVSITASMGYGSGLVVPGTGIPMNNTLGEPELNPRGFHA
                     LAPGQRLISNMSPTVVSSEEGSIIALGSPGASRIPTAILQTVINVLDFGKTLEEAVLA
                     PRFHAEGDLFAYEAGARTADLDNYERVLSYETPNMFFGGVNAVRRTPAGRFEAAADPR
                     RSGGTAIV"
BASE COUNT          422 a          905 c          796 g          485 t
ORIGIN      
        1 cgacgagctc gactgcgatt gtggctgtgc cgttcttgac gtccaggatg gggttcactt
       61 cgaccacgtc gagcgaagaa attatcccgg cctcgttgac cagctccatc agcaggtgcg
      121 cctccctgta ggtgagtccg ccgcgcacgg gtgtgcctac gccgggggcg acttcggggt
      181 cgacggcgtc caggtcgagc gagaggtgca ccctgtcgac gtccgtgagg cccttgatgg
      241 cgcttcgcac gacgcgcgcc gcaccgtagg cgtcgatgtc cttcatagtg tagaccctca
      301 cgccggcctc ccgcagcagc cgcttctcct caacatccac ggagcgcaac ccgatgatca
      361 cgacgtcctc tgtcctgacg ctggccccag gactgccgat cccgacgagg tcagggtggc
      421 cgcgccccgt tagggttgcc agcggcatgc cgtggatgtt gcctgatggc gaggtcgcag
      481 gggtattgaa gtccgcgtgc gcgtcgaccc agatcacacc cgtccgctct cccgatgcgg
      541 agcgcgcaat cccggagaca gtaccgatgg cgattgagtg atccccgccg aggaagatcg
      601 ggaacatccc ttctgagact atggccgcgg cccgctcgga gacctcttca cagacactct
      661 tcacagcggc gaggtgcttg acctccgccc ccgccacgac ggtctcgggg attggtaccc
      721 ctgcgttgcc gaggtcggta accctgtgcc ccagttcctc caggcatgcc tcgatacggg
      781 cgtaacggat ggcgctgggt cccatatcca cgcctcgccg gtcctggccg aggtccatgg
      841 gcacaccgaa gatccccacc ggcccgcgcc cgctttcaaa caatggcagt tcctcccgaa
      901 cgacggggat cggccgccgc ctcgaagcgt ccggcaggtg ttcgcctgac cgcgttgacc
      961 ccgccgaaga acatgttggg ggtctcgtag ctcagcacgc gctcgtagtt gtcgaggtcc
     1021 gccgtgcgcg caccggcctc gtaggcgaag agatcccctt ccgcgtggaa gcgcggggcg
     1081 aggaccgcct cctccagtgt cttcccgaaa tccagcacgt ttatgacggt ctgtaatatt
     1141 gccgtaggta tgcgcgaggc gcccggcgat ccgagcgcga tgatgctccc ctcctcggag
     1201 gagacgacgg tcgggctcat gttggagatc aagcgttgcc ccggcgcgag agcgtgaaac
     1261 cctctagggt tgagctcggg ctcgccgagc gtgttgttca ttgggattcc ggtgccaggc
     1321 acgacgagcc cggacccgta gcccatgctg gccgtaatag agactgccaa accctcgctg
     1381 tccacgcacg cgagatgtgt ggtatgcggt gagccgaacg tggagaagat cctgcgtcgc
     1441 tgctccgccg cgtactcctc ccctgtcagc cgctcggcga cccggcggtt ctccgaaccg
     1501 tcgagatagg ccgtctcgcg atcctcgagc gccagcttca tcgccccggc catcaccctc
     1561 acgtactctg cttcaggcaa gctcgccaga tcgtaggcgg agacgacctt gagcatctgg
     1621 gccagcgtcg ggccgccggc ggaaggcggg ccgttggtat gcatggcccc agcgccgtac
     1681 ctgacgctca tcggctccct gatgacggcc tcgtactcgg cgaggtcctc ttcggttatg
     1741 atgccgccca tcttcagcat gtaggaggag atcgtgcgcc ccagcccccc ctcgtagaag
     1801 agatccgccc caccctcccc gatcgcctcc agcgtgtcgg cgagctcggg gaaccgtacc
     1861 tcttcaccct cgaggtacac ccgctcccct ttgtagaagt gcttgcgggt ctcctcggtg
     1921 agcctcagca cctcctcggc gaccccaaac cagagggccg aggtcttgca caacctgaac
     1981 ccttcgcgcg cgaacctgac cgccggggct agtgcctcct tcatgccgag ccggccgtgg
     2041 cgcgccagga gtttctccca ccccctcaag gagcctggta cggctgacga tgcccccccg
     2101 acgatggatc gcaccccggc tccgtacttg agaatgatgg tctgcgggct gccgccggcc
     2161 ccgaaggccg tgtcagggag ccccttgccg ggcatcgcat cgtagtagtc gataagctcc
     2221 gccccgccgg caggatctct aacgagggcg aacccgccgc ccccgatcga actcatcagc
     2281 ggctcggtaa cgctgacggc ggccgccgcg gctacgcccg catccgcggc gttgcctccc
     2341 gccaggtacg tctcggcggc ggcctcggcc gcgtacggag tacccgcggc ggcggcgaag
     2401 gtgctgttag ggcctatagt ctccataatt ggctattatg cacagtcttt accgcgtcgt
     2461 gccgtctaag gctgaaaaat gccttctcta aaggtgattt ggtccatgca ggccaattct
     2521 atgtaccgct caatatcgct gtttactgct cctgttgctg ccacgctgct gccataaggc
     2581 tgccggttat gaagacccat cctcgctc
//
LOCUS       DASTKM010000011         4621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_2248, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000011 DASTKM010000000
VERSION     DASTKM010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2248"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1051
                     /locus_tag="VFQ10_00375"
     CDS             32..1051
                     /locus_tag="VFQ10_00375"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET9963820.1"
                     /translation="METRNLKMHFPIKAGVLQRTVGHVKAVDGVDLSVRRGETLGLVG
                     ESGCGKSTLARLVLRLLEPTEGEVIFDGEDILGYDRKQMLGVRRNMQIVFQDPYASLN
                     PRMTVGNIVSEPLKTHDVGGSSGERKRRVQELLEVVGLNPEHYNRYPHEFSGGQRQRI
                     GVARAIALNPRLIICDEPVSALDVSIQAQVINLLEDLQKEFDLTYIFIAHDLSVVKHI
                     SDRVAVMYLGKIVEISDKPTLYSNPRHPYTASLLSAIPIPDPQKERERERVVITGDVP
                     SPANPPSGCNFHNRCPRALDYCSEHEPGLDSQEKVDHKAACFFPVLEGQRIEEPPTST
                     PYRAE"
     gene            1368..2612
                     /locus_tag="VFQ10_00380"
     CDS             1368..2612
                     /locus_tag="VFQ10_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9963821.1"
                     /translation="MWKDTKTRRDLRNASARVLLLVVLVLATAFGAYMVGRSQSPATL
                     NEKDKESVALYAEALDTVRDNYVDQKNIDSKKGTYGAIEGMLDTLGDGGHTRFLTPTE
                     REQNDRSLSGTYVGIGVQLEEKNGEVVVAAPIDGSPAEEAGISSDDVLLAVDGKSVRG
                     DEVSEVVEKVKGPEGTSVELTVRHEGERRTYDLQRAEIDSPVASWAMIPGTNVALVLL
                     SSFSDDSAQELQNAFEEAQASGARRFILDLRNNPGGRLDQAVEMAGYFLEPESVVYIR
                     KDASGEREEITVEGDPESTDAPLAVLVDGGSASSAEILAGALRDNDRASVVGETTFGT
                     GTVLSEFVLRDGSSILLGVAEWLTPDGDFIRNSGITPDVKVSLSEGTEPLTPDDARDL
                     SRQDILEKDAQLRTAYEKLQGQ"
     gene            2692..3159
                     /locus_tag="VFQ10_00385"
     CDS             2692..3159
                     /locus_tag="VFQ10_00385"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9963822.1"
                     /translation="MAQRVHESIEVDAPVDDVFRYWSNFEHFPTFMSNVEEVRMSAKD
                     TSHWRVKGPLGKSVEFDAKTTEMDPNRGIGWNTVDGEVMTSGEVRFGETTPGRTQVDV
                     TMNYADPPGGSVGETVANILSNPERNLKEDLQNFARIVERGELGGSEAQTPSR"
     gene            3283..3435
                     /locus_tag="VFQ10_00390"
     CDS             3283..3435
                     /locus_tag="VFQ10_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963823.1"
                     /translation="MSIAIVSLLVLVVVVIIAVPILIRVFGEAARRNPNEEEDESTER
                     ESNHNS"
     gene            complement(3478..4572)
                     /locus_tag="VFQ10_00395"
     CDS             complement(3478..4572)
                     /locus_tag="VFQ10_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9963824.1"
                     /translation="MQPQRDLRDIEEIQELFEAGQETGTLESSEVLDLLQEVDLSTDE
                     IQQVYGLLREHGVEVVDAGFLAETMGEDEDTQLVKEVLEGDLKSRYTGDAVQMYLDEI
                     GKTPLLTKAQETYLARRIERGDRRAKAHLTKANLRLVVSIAKKYAGRGVSLLDLIQEG
                     NIGLMRAVEKFDYRKGFKFSTYATWWIRQAVTRAIADKGRTIRVPVHMVEKINKYYRV
                     QRGLGAQLNRDPTDEEVASIMEVDAEEIVRIRRVSRRSISLETPVGEDHSSELGDFIA
                     DEESESPHDLAKSSLLKARMREALGSLPERERMVLEYRFGLTGGQPKTLEEVGERFDV
                     TRERIRQIQLTALAKIKSSPHAAGLRDLLE"
BASE COUNT          894 a         1446 c         1449 g          832 t
ORIGIN      
        1 acagaccaac ggtcattccg gcgagtatct gctggagacc aggaacctga agatgcactt
       61 ccccataaag gctggcgtgc tgcaacgcac ggtcgggcac gtcaaggctg tggacggcgt
      121 cgacctctcc gtgcgccgcg gcgagacgct cgggctcgtg ggagagtcgg ggtgcggcaa
      181 gagcacgctc gcccgcctcg tcctgcgcct gctggaaccg acggagggcg aggtgatctt
      241 cgatggcgag gacatactgg gttacgaccg caagcagatg ctcggcgtgc ggaggaacat
      301 gcagatcgtc ttccaggatc cctacgcgag cctgaatccc cgcatgaccg taggcaatat
      361 cgtctccgag ccgttgaaga cccacgacgt aggcggtagc tccggcgagc gcaagcgacg
      421 ggtgcaggag cttctcgagg tggtcggcct gaaccccgag cactacaacc gctacccgca
      481 cgaattctcc ggaggccagc ggcagcggat cggggtggcc agggcaatcg cgctcaaccc
      541 gcgcctgatc atctgcgacg agccagtctc ggctttggac gtctcgatcc aggcgcaggt
      601 catcaacctg ctggaggacc tccaaaagga attcgacctg acctacatct tcatagccca
      661 cgacctctcc gtggtcaagc acatctcgga cagggtggcc gtgatgtacc tcggcaagat
      721 cgtggagatc tccgacaagc cgacgctcta cagcaatccg cgccacccct acaccgcctc
      781 gctgctctca gcaatcccga tccccgaccc ccagaaggag cgcgagcggg agcgcgtagt
      841 tatcaccgga gacgtgcctt ctcccgccaa cccacccagc gggtgcaact tccacaaccg
      901 ctgtccgcgg gcgctggact actgctccga gcacgagcct ggtctcgact cgcaggagaa
      961 agtggaccac aaggctgcct gctttttccc cgtgctcgag ggccaacgca tagaggagcc
     1021 ccctaccagc acgccgtacc gggctgaata gcatttcagc ccgctccggc ttacgcctcc
     1081 gcttgtcagc acggcgcttc gcgccttgtc agcacgcttc gggtcttcga ccctcgctct
     1141 cagcatgtca gcttctcccg tcccagcctt gcccggccgc gcacgaactg ctgcagcccg
     1201 cgtggtcggg ttctaccctc gtggcggcgc ctcaggacga cttgactgaa ggctgagagc
     1261 cgccgaccgc agggagacgg cgtgctgaca agcgcccgac agggcgcttg ctgaaatgct
     1321 gaccagcgga gcccgtaggg cgcagcgggc tgagatacaa ttggcgcgtg tggaaggaca
     1381 ccaagaccag acgtgacctg cggaatgcgt cggcgcgggt gcttctcctg gtcgtcctgg
     1441 tgctcgcgac cgcattcggg gcctatatgg tgggacgctc gcagagtcct gccacgctca
     1501 atgaaaagga taaagagagc gtggcgctgt atgccgaggc gctcgacacc gtcagagaca
     1561 actacgtaga ccagaaaaac atagattcca agaaggggac gtacggggcc atcgaaggga
     1621 tgctcgacac gcttggggac ggggggcaca cgcgtttcct gactcctacg gagcgcgagc
     1681 agaatgaccg gagcctctct ggaacgtacg ttgggatcgg ggtccaactg gaggagaaga
     1741 atggtgaggt cgtggtcgcc gccccgatag acggctctcc ggccgaggag gctggtatca
     1801 gttccgacga cgtgctgctg gccgtagacg ggaagagcgt caggggtgac gaggtctcgg
     1861 aggtcgtgga gaaggtcaag ggaccggagg ggaccagcgt cgagttgacc gtccgccacg
     1921 aaggcgagag gcgtacgtac gacctccagc gcgcagagat agactcgccc gtagcttcgt
     1981 gggccatgat ccccggcacg aacgtcgccc tcgtgctcct ctcgtcgttc tccgacgaca
     2041 gcgcccagga gctgcagaac gctttcgagg aggcgcaggc ttcaggagcc aggcgcttta
     2101 tcctcgacct cagaaacaat ccaggcggca ggctcgacca ggcggtggag atggcaggat
     2161 acttcctcga gccggagagc gtcgtctaca tccgcaagga cgcttccggc gagcgcgagg
     2221 agatcactgt cgaaggcgac ccggaatcga cggacgcgcc actcgctgta ctggtcgacg
     2281 ggggttctgc ctcgagcgcc gagatactgg ccggagcctt gcgggacaac gaccgcgcgt
     2341 ccgtcgtcgg ggagaccacc ttcggcactg gcaccgtgct ctcggagttc gtcctcaggg
     2401 acggctcatc tatcttgctc ggcgtcgccg agtggcttac cccggacggc gactttatcc
     2461 gcaactcagg gatcacaccc gacgttaaag tgtccctctc cgaggggaca gaaccgctca
     2521 cgcccgacga cgcccgcgac ttgtcccgcc aggatatcct cgagaaggac gcccaactgc
     2581 gcacggccta cgaaaagctc cagggtcagt agtgaggttc acataagaac gggcctggac
     2641 caggcatcct cacgggcaag atgaatctcg tagccaacag aaaggatgct tatggcacag
     2701 cgagtacatg agagcatcga ggtagatgcc cctgtcgatg acgtcttccg ttactggtcc
     2761 aacttcgagc acttcccgac cttcatgtcc aacgtcgaag aggtgaggat gtccgccaag
     2821 gacaccagtc actggcgggt caaaggtcct ctcggcaaga gcgtagagtt cgacgcgaag
     2881 acgaccgaga tggaccccaa ccgcggcata ggctggaaca cggtggacgg cgaggtcatg
     2941 accagcggcg aagtccgctt cggggagacc actccaggtc gtacgcaggt cgacgtgacg
     3001 atgaactacg ccgacccgcc cggtgggtca gtaggcgaga ccgtagcgaa catcctctcc
     3061 aacccggagc ggaatctaaa ggaggacctg cagaacttcg ccaggatcgt cgagcgcgga
     3121 gagctgggcg ggtcggaggc ccagacaccg agccgctgac cagcgcgaga tggggaaggt
     3181 caatccttcc ccatcccgct gtgggtaccg aaggcgctat tgtagggtag tatagaggtg
     3241 taagtccggc ggcctcaata gcctcgcggg gaggtgaatc tcgtgtctat cgccatagtc
     3301 tctctgctcg ttctcgtagt cgttgtgatc atcgccgtac ccatactgat ccgcgtcttc
     3361 ggcgaggccg cccgccgcaa cccgaacgaa gaagaggacg agagcaccga gagagaatcc
     3421 aaccataaca gctagtcagc atgtcagctt gcgccggccg gagcgtgctg actagctcta
     3481 ttccagcaga tcccgcagtc cggcggcgtg agggctgctc tttatcttgg ccagggctgt
     3541 gagctgtatc tgacggatac gctcgcgggt gacgtcgaaa cgttccccga cctcttctag
     3601 ggtcttgggc tggcccccgg taaggccgaa gcgatactcg aggaccatcc gctcgcgttc
     3661 ggggaggctg ccgagcgcct cgcgcatgcg ggccttcaag agggaggact tggccaggtc
     3721 gtggggagat tccgactcct catccgctat aaagtcaccc agctccgagg agtggtcctc
     3781 cccgaccggg gtctccaggg agatcgaacg tctcgatacg cgcctgatgc gtacgatctc
     3841 ctcggcgtcg acctccatga tggaggccac ctcttcgtcc gtggggtcgc ggttgagctg
     3901 ggcgccgagt ccgcgctgga ccctgtagta cttgttgatc ttctcgacca tgtgcacggg
     3961 gacccggatc gtacgtcctt tgtcggctat cgccctggtg acggcctgac ggatccacca
     4021 cgtcgcgtag gtcgagaact tgaatccctt gcggtagtcg aacttctcga cggcccgcat
     4081 caacccgatg ttgccctcct ggatcaaatc gagcagcgag acgccccttc cggcatactt
     4141 cttcgctata gagacgacga gcctgaggtt ggctttggtt aggtgggctt tggcgcgcct
     4201 gtcgccgcgc tctatgcgcc gcgcgaggta cgtctcctgg gctttggtga gaaggggggt
     4261 cttgcctatc tcgtccaggt acatctggac cgcgtccccc gtgtagcggc tcttcaggtc
     4321 gccttcgagc acctctttga cgagctgggt gtcctcatct tcgcccatcg tctccgcgag
     4381 gaatccggcg tctaccacct cgacgccgtg ctcgcggagg agaccgtaga cctgttggat
     4441 ctcgtcggtc gagaggtcga cctcttgtag gaggtcgaga acttcactcg actccagcgt
     4501 gccggtctcc tgaccggcct cgaagagctc ctgtatctcc tctatgtccc gcaggtcacg
     4561 ttgaggctgc aaaatctact ccgggaactt caggttgaaa ccctcctgta gccttcacgt
     4621 a
//
LOCUS       DASTKM010000012         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_354522, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000012 DASTKM010000000
VERSION     DASTKM010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354522"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFQ10_00400"
     CDS             <1..364
                     /locus_tag="VFQ10_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963825.1"
                     /translation="RNEALVALAAALCDVLALEYDSGGQMPIIPNREIEENKWSAQRY
                     GLEGIFIDHQTHEAVETRTAVETLVGRLSRATYRDLSGIERALGGPTESVRQLEVWRE
                     TGSTLEIAKDLARRASTL"
     gene            complement(370..501)
                     /locus_tag="VFQ10_00405"
     CDS             complement(370..501)
                     /locus_tag="VFQ10_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963826.1"
                     /translation="MKRAIAIKLIKSKRARRVALKAMKNPRIRKIVVDQARRRVLGK"
     gene            complement(515..1309)
                     /locus_tag="VFQ10_00410"
     CDS             complement(515..1309)
                     /locus_tag="VFQ10_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963827.1"
                     /translation="MSDRPEERRRTGPLGDEDDETRRVPTGNGPEATRQVREEAASAE
                     TRGIAQGPSEENETRVIRVPGHPDTTRDATPYPRGYFEATEEREDRLRDMYGGVDWLA
                     SFLGLVFAIVLGAVFSAVAGIVLVPFGITPALSGGQIGASVITGLALLGVLIFLTFFF
                     GGYVAGRLARFDGGRNGAMVLVWMFIVVLILALAAALFSGFLPAGMPEGIANLVDRIV
                     STAGNLAGAGVVGLVTAVAALLIALLGGFLGGRMGSRYHTEIDRAT"
     gene            1591..2922
                     /locus_tag="VFQ10_00415"
     CDS             1591..2922
                     /locus_tag="VFQ10_00415"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET9963828.1"
                     /translation="METADRQSVLRQAEENNVQFIRLWFTDVLGTLKSFAITVEELED
                     ALDGGMGFDGSSITGYQDIEESDMIAMPDPSTFQVIPWSPQDAPTARMICDVRTPDGD
                     PYVGDPRHVLRRALERASEMGFDKFYCGPELEYFYFRDSKGTEPLDYGGYFDLTTLDA
                     ATALRRETVQSLQQLGIKVEYSHHEVGISQHEIDLRFDDAMAMADKVMTYKTVVKEVA
                     TRHGVYATFMPKPLRDQNGSGMHTHQSLFSGGKNAFFDADNEYFLSSEAQSFIAGQLR
                     HAREISIIFAQHVNSYKRLVPGYEAPVYIAWSRRNRSALVRVPLYHPGQERATRMELR
                     CPDPAANIYLCFAALLHAGLEGIEKGYELPEPMERNLYHLTAEEREKMGIESLPADLG
                     EAIKEAENSELLHKALGEHTYTRLLQLKREEFEDYRVQVTPYELEKYLPVL"
BASE COUNT          624 a         1037 c          977 g          547 t
ORIGIN      
        1 ccgcaacgag gccctcgtcg ccctggccgc ggccctgtgc gatgtgctgg ccctggagta
       61 cgactctggc gggcagatgc ccatcatccc gaaccgcgag atagaagaga acaagtggag
      121 cgcccagcgt tacggcctgg aaggcatctt catagaccac cagactcacg aggccgtcga
      181 gacccgtacc gctgtagaga cgctagtggg gcgcctctcc agggccactt acagagatct
      241 ctcgggcatc gagcgcgcac tcggtgggcc gacagaatcc gtgcgtcagc tagaggtgtg
      301 gcgagagacg ggatccacgc tcgagatcgc gaaggacctg gcgaggaggg cctcgacgct
      361 ctagcgccgc tacttcccca gcaccctgcg cctggcctgg tccacgacga tcttcctgat
      421 tctggggttc ttcatggcct ttagggccac ccggcgcgcc ctcttgctct tgatcaattt
      481 gatagcgata gcccttttca cgactcgcct ctggtcaggt cgccctgtct atctcggtgt
      541 ggtacctgct gcccatacgc ccgcccagaa agccgccgag gagcgctatg agcaaggcgg
      601 ccacggcggt tactaggccg accacgcccg caccggccag gtttccggcc gtggacacga
      661 tcctgtcgac caggttggcg atgccctcgg gcatcccggc gggcaggaag ccgctgaaga
      721 gcgcggccgc cagagcgagg atcaggacaa cgataaacat ccagaccagc accatggccc
      781 cgttgcgccc cccgtcgaaa cgcgccaggc gtcctgccac atacccgccg aagaagaaag
      841 tcaggaagat caagacccca agcagggcga ggcccgttat gacggaggcc ccgatctgcc
      901 cccccgaaag ggcaggcgtg atgccgaagg ggacgagcac gatccccgcg acggccgaga
      961 agaccgcgcc gaggacgatg gcgaagacga gcccgaggaa gctcgccaac cagtcgacac
     1021 ccccgtacat gtccctgagc cggtcctccc gctcctccgt agcctcgaag taacccctcg
     1081 ggtagggtgt cgcgtccctg gtcgtgtcag ggtggccagg caccctgata acgcgggtct
     1141 cgttctcctc ggaaggcccc tgggcgatcc cccgagtctc ggcggatgcc gcctcctctc
     1201 ggacctggcg cgtggcctcg gggccgttcc cggtgggcac gcggcgcgtc tcatcgtcct
     1261 catctccaag aggtccagtt ctgcggcgct cttccggcct gtcgctcatc tctcctcctt
     1321 acgtcatatc ttaagtcgtg cgtctgttgt acagtgtaga ttctaccctg caagtgccaa
     1381 gggcacccgc gaggccgccc cggcacagcc ccgcatgatg acccgagggc caatttttgt
     1441 acatcaggcc gagaattggg ccggatcgcg gtgcctggct tgtacgcgcg gccatatgca
     1501 aggggtgggg acccttcata tcatggccgg ggttgcaggc gagtcccggc cccgggactt
     1561 ggtgaacaac gaatatccga ggaggcaagc ttggagacgg ctgacaggca gagcgtgttg
     1621 cggcaggcag aggagaacaa cgtacagttc atccggcttt ggttcaccga cgtgctcggg
     1681 acgctcaaga gcttcgcgat cacggtcgag gaactagaag acgcccttga cgggggcatg
     1741 ggattcgacg ggtcttcgat cacgggctat caggacatcg aggagtccga catgatagcc
     1801 atgcctgatc cctcgacctt ccaggtcatc ccctggagcc cgcaggatgc gccgacggcg
     1861 cgtatgatct gcgacgtcag gacgcctgac ggcgatcctt acgtcgggga cccgcgccac
     1921 gtcctgaggc gcgccctgga gcgggcgagc gagatgggct tcgacaaatt ctactgcgga
     1981 ccggagctag agtacttcta cttcagggac tccaaaggga cagagcctct cgactacggc
     2041 ggctacttcg acctgacgac gctcgacgcg gcgacggcct tgaggcgcga gactgtgcag
     2101 tctctgcagc agctcggcat aaaggtggag tactcccacc acgaggtcgg catctcccag
     2161 cacgagatcg acctccgctt cgacgacgct atggcgatgg ccgacaaggt gatgacctac
     2221 aagacggttg tcaaagaggt cgccacccgc cacggagtct acgctacgtt catgcccaag
     2281 cccttgaggg accagaacgg ctcggggatg cacacccacc aaagcctgtt ctcgggcggc
     2341 aagaacgcct tcttcgacgc ggacaacgag tactttctct ctagcgaagc ccagtcgttc
     2401 atcgcgggac agctgaggca cgcgcgcgag atcagcataa tcttcgccca gcacgtaaac
     2461 tcttacaagc ggctggtccc cggctacgaa gcccccgtct acatcgcctg gagccgtcgc
     2521 aaccgctccg cactggtccg cgtccccctc taccaccccg gccaggagcg cgcgacccga
     2581 atggaactac gctgccccga ccccgcggcg aacatctacc tctgcttcgc ggcgctcttg
     2641 cacgccggcc tggagggcat cgagaagggc tacgagctgc ccgaaccgat ggagcgcaat
     2701 ctctaccatc tgacggccga ggagcgcgag aagatgggaa tagagagcct tcctgccgac
     2761 ctgggagagg cgataaaaga ggcggagaac tcggaactgc tgcacaaggc cctcggtgag
     2821 cacacctaca cccggctcct gcagctcaag cgcgaggagt tcgaagacta cagggtgcag
     2881 gtaaccccct acgagctcga aaaatacttg ccggtcttgt aggtttcagg ctacaggtct
     2941 caggttgggg tccggctcac gccgggcccc tcgtgctcgg gccccagcgt gtttacaaga
     3001 gttgtcggac gggtcggacc aacaaggata gattcctttt gtctgtaccc agagaaaccc
     3061 tgtaacctga tgccttgagt cgcactagcg gctttcgatg agtctcagaa cctcgggaag
     3121 gagtgacctt gtggtgctca tcgcttcacc cgagtagatg gtctcgttgc cgaaccagtc
     3181 gccga
//
LOCUS       DASTKM010000013         3577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_2376, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000013 DASTKM010000000
VERSION     DASTKM010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            578..1174
                     /locus_tag="VFQ10_00420"
     CDS             578..1174
                     /locus_tag="VFQ10_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HET9963829.1"
                     /translation="MAEAGAVPGYSFGAQATARSPLGLEELDLLKQTVLFTEEDEEYL
                     RLAGDVLEDQVDEVLDLWYGFVGSHPHLIRYFASPDGEPNARYLERVRERFKRWILDT
                     CRRPYDQQWLDYQQEIALRHTRAKKNQIDGAQETPEHIPLRYVIAFVYPITATIKQFL
                     AKGDHAADVVEKMHYAWFKSVTLQVALWSQPYTKEGDF"
     gene            1176..1832
                     /locus_tag="VFQ10_00425"
     CDS             1176..1832
                     /locus_tag="VFQ10_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET9963830.1"
                     /translation="MGVAWGRSAAGTHWSAFEPSTQHENVDKKIVASLERLSQVFRIL
                     LRGEAQERGLSPIQARFLIHLLHHGPTLRRVSQLAREFDLSRATVSEAVGSLEKKGLI
                     RREPWPHDKRVTTLRLTPTGEHTARELAAWANVVEDHLEGRSPEEKEAVMRFLMDLIG
                     SLQKSGLITVARMCVTCRFFRPDAHPGESSPHHCALLNVALSGSDLRADCPEHKLAAS
                     "
     gene            2336..2908
                     /locus_tag="VFQ10_00430"
     CDS             2336..2908
                     /locus_tag="VFQ10_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963831.1"
                     /translation="MRFVTVLAAVIFLVGCSHAAPSVEKQHKREAPERIEKVPPKGPS
                     PELPAYDITSEQDCAESGVVGKCYGVSTDATSREELEVITADLWLDSPGYLAVLVTFY
                     PNKPTADLSAWGFAFENEQAARVVLAQFLSEGTSVEDEVREAMANGGIYIVVPADETR
                     ELTQESAVGRLGEAPARCVAPTAFKWAHAS"
     gene            complement(3016..>3577)
                     /locus_tag="VFQ10_00435"
     CDS             complement(3016..>3577)
                     /locus_tag="VFQ10_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET9963832.1"
                     /translation="VGYVMTAIFMVAMLVVGAELLFASGASISEEEGLVALADPIQER
                     FGVVARWLFLVGFWAAATSSIVGAWNGSAYLFADSVRTSRDVPEERTEEYLSEKSVYF
                     RGMLVWITFPPMLLLFFQQPVLLIIVYAAMGALFLPFLAITLLWLLNSRRVAPEHRNG
                     IVSNIILVASVLLFVFLGIQEVLGAA"
BASE COUNT          762 a         1083 c         1122 g          610 t
ORIGIN      
        1 ccccggacac gcccgcgaga tcgcagcgtg ggtggccttc ctcgcctcgg agaagtcatc
       61 ctacgcaacc ggcgcctcat tcgttgtgga cggcggcctg aagctgatgg ctgcagagtt
      121 gtcgagctag gctaccagaa cgtcaactca ccggaccatc gcgaatctcg tctttgcccg
      181 aagcgcctac catagagctg agagcgcctt tgccaaagac ggcgtcgtcg gggtacccct
      241 acttgtgcgg cggcggatgt gccatcagga atcgcaaccg acttcaggtg ggtccacgca
      301 agtcgggaca acgccgagcg tgtacttcca ggctactcta gtcgaggaag gtacgtgcac
      361 aaagctggaa gtcgcgctct agccacgcgg acgtgggtag cgcacgcgag cctcgcgagg
      421 aggcggccaa ggcgaagggg gataggaggg tgcggcctcc tccgacaccg caatcgcagt
      481 gtcaaaaccc cttgatcgaa atcggcaatt actatattgt taggactcct aacaacgtgg
      541 ccaaattatg gtcgggagaa gaagcaggga ggcacaaatg gctgaggcag gggcggtccc
      601 cggatatagt ttcggcgcgc aggcgacggc caggtcgccg ctcggtctgg aggaattgga
      661 tctgctcaag cagacggttc tcttcaccga ggaagacgag gagtacctaa ggttggcggg
      721 tgatgtcttg gaggatcagg tcgacgaggt cttggacctc tggtatggct tcgtcgggtc
      781 gcacccgcac ctgatccgct acttcgccag ccctgacgga gaaccgaacg cgcggtacct
      841 cgaaagggta cgcgagcgtt tcaagcggtg gatcttggac acctgccggc gaccttatga
      901 ccagcagtgg ctcgactacc agcaggagat cgccctgcgc cacacgcgcg ccaagaagaa
      961 ccagatcgac ggagcacagg agactccgga gcacattcca ttgcggtacg taatcgcctt
     1021 cgtctacccg atcacggcga ccatcaagca gttcctcgcc aaaggggatc acgccgccga
     1081 cgtggtcgaa aagatgcact acgcctggtt caagtcggta accctgcaag tcgccctctg
     1141 gagtcaaccg tacacaaagg aaggggactt ctagcttggg cgtagcgtgg gggcgctctg
     1201 cggcgggcac acactggtct gccttcgagc cgtccactca gcacgagaac gtagacaaga
     1261 agatcgtcgc ctcgcttgaa agactctccc aggtcttccg gatcctcctc cgcggagagg
     1321 ctcaggagcg tggtctgagc ccgatacaag cccggttcct catccacctg ctccaccacg
     1381 gccccacgct ccgacgcgtg agccaactcg cccgggagtt cgatctcagt cgggccacgg
     1441 tgagcgaggc cgtcggttcg ttggagaaga aaggtttgat ccgcagggaa ccttggcctc
     1501 acgacaagcg cgtcacgacg ctacgcttga cccctaccgg ggagcacaca gcgcgagagc
     1561 tcgctgcctg ggcgaacgtt gtcgaagatc acctagaggg ccgttcccct gaggagaagg
     1621 aggcggtgat gcgcttcctt atggacctga ttggctcctt gcaaaagtcc ggcctgataa
     1681 ccgtagcgag gatgtgcgtg acctgccggt tcttccgtcc ggacgcccat ccgggcgaga
     1741 gttcgcctca ccactgcgcg ctgctgaacg tggcgctctc cggctccgac ctgagggccg
     1801 attgcccgga gcacaagctg gccgcgagtt gagtcttggg gcaactaatg ctgcgttaca
     1861 ctggccagga agctcccgtt gagcacgatc tggcacgcac tacacatcgt tgttctcggc
     1921 tcatgcggcg gaggcgtcag gcggcgagac gcgggcgtca atcaaccagt acgtcgaact
     1981 cgctaccaga gcctacagct actgcagccg ctgagaccgc ctcacccggc ggtataccaa
     2041 cgagcgaact atttccgaga actccctacg cccgaagttc tggcaactcc tcttccacgc
     2101 actcgagtga ctaggagctg cggaccgact acttcctctc gccacttacg accgttcgcg
     2161 ggtaagcagc gcggcgtatc ctccgactcc tcgggcgacg ggacgacgcc taacgttgcg
     2221 ctgaattacg cgtaggtgaa cacacggtcc ggatctgtta cctgtggcac atcagccaga
     2281 agcacggggc acaactacga aaaagctgtc cgctcgagta taagatgggt aaacagtgcg
     2341 gttcgtgacg gtattggctg cggtgatatt cttggttggc tgcagccacg ccgcgccatc
     2401 tgtcgagaag cagcacaaga gagaagctcc agagcggata gagaaggtcc cgccaaaagg
     2461 gccatctccc gagctaccgg cctacgacat aacctcggag caagactgcg ccgagagcgg
     2521 cgtcgtgggc aaatgctacg gcgtctccac tgatgccacc tcaagagagg agttggaagt
     2581 catcaccgcc gacctctggc tcgatagccc tgggtacctg gccgtcctgg tgacgttcta
     2641 ccccaacaag ccgaccgcag acctcagcgc ctggggcttc gcgttcgaga acgaacaggc
     2701 cgcccgcgta gttctcgcgc agttcctctc cgaggggacc agcgtggagg acgaggtgcg
     2761 tgaggcgatg gccaacggcg gcatatacat cgtcgtacca gccgatgaga cacgggagct
     2821 tacccaggag agcgccgtag gccgcctggg ggaggcaccg gcgaggtgtg ttgctcctac
     2881 cgccttcaag tgggcccacg caagttgaga caacgccatc cctgatgtgc cgagcgctgc
     2941 tcaggcctga tggcgaagta gatttgggcg tagcgctcta gcaccgaaga ggactacgaa
     3001 aaggcaaagg gtgatctagg cggcgccgag aacctcttgt atcccgagaa aaacgaagag
     3061 caacaccgag gctaccagga ttatgttcga gacgatgccg ttgcggtgtt cgggggccac
     3121 gcgccgggag ttgaggagcc agagcagcgt tatcgccagg aagggcagga acaaggcacc
     3181 catggcggcg tagacgatga tcagaagcac cggctgctgg aagaagagca ggagcatcgg
     3241 agggaaggtg atccagacca gcatcccccg gaagtagacg ctcttctcgg agagatattc
     3301 ctccgtcctc tcctcgggca cgtcgcgaga ggttctcacc gagtcggcaa agaggtaggc
     3361 cgagccgttc caggccccga ctatagagct ggtcgcggcg gcccagaatc cgacgaggaa
     3421 cagccagcgc gcgacgacgc cgaagcgctc ttgtatggga tcggcgagcg ccacgagccc
     3481 ttcctcctcg ctgatgctcg cgccgctggc gaagaggagt tcggccccga cgaccagcat
     3541 cgccaccatg aagatggcgg tcatgacgta gccgacg
//
LOCUS       DASTKM010000014        12401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1235558, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000014 DASTKM010000000
VERSION     DASTKM010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12401
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235558"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(477..1199)
                     /locus_tag="VFQ10_00440"
     CDS             complement(477..1199)
                     /locus_tag="VFQ10_00440"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9963833.1"
                     /translation="MRAVEDLEASLSLYREVGDRRGEAYALCFLGWIALDRSDLQRAE
                     ALLEESLALSRAAGTARDVSVALNALAMLNVYRGDYARASAMQEESLSLAREASDVQI
                     IAILTYNTGFAAAITGEYERAEALVRESQELFREVGDRGMAPLANSRLGFLALSRNDP
                     DRAEELCVEAIRELQEQAQTPGMDFVLDVLASVAAARGGIRRAARPWGGVAGYREATG
                     GSLATRGARNHRAAHRCGALPA"
     gene            complement(1214..1447)
                     /locus_tag="VFQ10_00445"
     CDS             complement(1214..1447)
                     /locus_tag="VFQ10_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963834.1"
                     /translation="MDRLEAEHDNFRAALSRSLEAGDAESALRIGAPCGASGTSGATL
                     ARVGTGWEQVWQGDGPLLLASGARRRLIWGTWS"
     gene            complement(1434..2090)
                     /locus_tag="VFQ10_00450"
     CDS             complement(1434..2090)
                     /locus_tag="VFQ10_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963835.1"
                     /translation="MPGLEALSHYEAVRLFIERATAAKADFAVTSENAPAVAEICARL
                     DGLPLAIELAAARVKLLPPRAMLGRLGRRLKLLTGGARDLPERQRTLRGAIEWSHALL
                     DEGEKTLFARMAVFSGGRTLDAIEAVCDSGGDLPLDAFESVSSLLDMSLLRQEEGSEG
                     EPRFVMLETIHEYARERLQDSGEAEEIGRAHNTILPGCCGRGRARVDGCGSGVVDGPA
                     "
     gene            complement(2039..2725)
                     /locus_tag="VFQ10_00455"
     CDS             complement(2039..2725)
                     /locus_tag="VFQ10_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HET9963836.1"
                     /translation="MRDQLPVDAQLRDLGDHRLKDLARPERVFELLAPDLPSGFPPLR
                     TLESRLNNLPMQPTLLVGREREVGEVRGRLLAPEVRLLTLTGPGGTGKTRLALQAAAD
                     LLEEFEDGVFFVALATISDPALVVPTVAQALGVRESGDRPLTEGVRDYLKDKRLLVVL
                     DNFEQVLEAAPMSGELLSAPQVKVLATSRIPLGVYGEHEYTVPPLSVRTRRGCPVSRP
                     SPTTRRSGSS"
     gene            complement(2718..3137)
                     /locus_tag="VFQ10_00460"
     CDS             complement(2718..3137)
                     /locus_tag="VFQ10_00460"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9963837.1"
                     /translation="MVAPPSGTVTFLFTDIEGSSRMWEESPATMRAALARHDEILRSV
                     IEAHGGHVFKTVGDAFCAAFPTAPEALDVALAAQRALLVDRDWIEAGPPLVRMALHTG
                     SAEERDGDYFGPPLNRASVCGPRGSDAPLFAGPGAGA"
     gene            complement(3220..4059)
                     /locus_tag="VFQ10_00465"
     CDS             complement(3220..4059)
                     /locus_tag="VFQ10_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9963838.1"
                     /translation="MFAVSASAIFIRLAEAPAIAIAFWRCALGAAVLLPPALVRKDGF
                     PRGRALYVGIASGVALGAHFGFWISSLDYTSVAASVVLVSTQPVFVAVLAYLLFGERT
                     TPLSFLGIVVAIAGTAFIAFDRTVGSAAVLGNALALVGAVTVAVYVLIGRSSRTTGGV
                     GVLPYSIVVYSAAAAALLPFALSFDVRLWGYSGETWFWLAAITAGPQLMGHTVFNWAL
                     PYVQASIISGTILAEPVVAALLAWLVLSERPGLLTIVGGAVVLAGLYFLLKGRPVVTE
                     PVS"
     gene            complement(4068..4670)
                     /locus_tag="VFQ10_00470"
     CDS             complement(4068..4670)
                     /locus_tag="VFQ10_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET9963839.1"
                     /translation="MKGPFRGGILGLLRQLLRVYVGRPSTSAPDGKRARPRVVVVLGA
                     QVLPGGRPSPTLEVRTRHAGELYGRGLADLLVPTGGEGEHPPGEAVVMSDILREMGVP
                     EAAILREDTARNTWESAVRVAEIAHHRGIGEVLVVTDPLHCVRTVASFGRVGLLAVAE
                     PVYSSPMWRKKWSRRGQLVRESGALAWYRIRHGVGLLSLP"
     gene            complement(4667..5263)
                     /locus_tag="VFQ10_00475"
     CDS             complement(4667..5263)
                     /locus_tag="VFQ10_00475"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET9963840.1"
                     /translation="MSRPASGIILAGGASRRMGREKLSLEVEGVPLIERVRDALTGCC
                     QEVLVVGDGGVRLEGVRYITGERPGGLGPLSGMEAGFASARYPLTFVAAGDMPFLTQS
                     MVSYLLGRLARGRVLAAVPRYGSRIHPLCAAYARTLLPRLGTALDGGARAVQGFIEGV
                     DEVEYVGEELRRFGDPGLLLMNVNSPGDLALARREARR"
     gene            complement(5260..6249)
                     /locus_tag="VFQ10_00480"
     CDS             complement(5260..6249)
                     /locus_tag="VFQ10_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET9963841.1"
                     /translation="MSLSGPLIWLGILQTVIYLLFIRTIDLYEREPLRYVIPVFIWGF
                     AVATTVSLVFNTLFQLTLSSVTSVKTASFFTAVVEAPVVEECSKGAALLLIFFIAYLA
                     RRRSGLVEFAGVMDGIVYGSAVGFGFSIAEDLLYGLEFGPETYIVRRIFGGFAHASFT
                     SLTGIGIGLIPLVDNRVLKVILPVLGLFGAILLHAAFNFTATVFGPVGYLVLFCVILV
                     YIVIIIVWLAIERRAIRDELSDEVRAGTITSQEYAILPSYFRRTGYYLGLLFTGRLRT
                     WSRVRKLHAAAVDLALAKRLGRRTSSPGSMDRVRMLRQKILSLREGATVRAVT"
     gene            6333..6827
                     /locus_tag="VFQ10_00485"
     CDS             6333..6827
                     /locus_tag="VFQ10_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET9963842.1"
                     /translation="MDRDLRDRDSILAALRHLTHGVYVMGTRRGRQSNAMTASWVMQT
                     SERPPCLAVAVRSDRYTHDIALESQTFALSVLREDQVDVATHFADTSGEYHDKLQGIP
                     YGLTPGGSPFLLDCLAYLDCKVIDRARAGDHTVVVGEVTASDTLDESYPLIYDPGEYE
                     EALR"
     gene            6909..7157
                     /locus_tag="VFQ10_00490"
     CDS             6909..7157
                     /locus_tag="VFQ10_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET9963843.1"
                     /translation="MDATLEKTGIRLFTGDYCPFCSRVRNELERLGLDYEVVDADADM
                     REEVIRLSGQRAIPILTIGDEVLVDSSHIIRELRNRYA"
     gene            complement(7148..7996)
                     /locus_tag="VFQ10_00495"
     CDS             complement(7148..7996)
                     /locus_tag="VFQ10_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963844.1"
                     /translation="MIWRDGEETPEVDGLEAAPEEKADPIQVERDAPRPATILRVAGE
                     LEERGGRIIELFKEIESPLGRVVLPIYLRQDERDIFVEVATGPWDDRMSGEALDKAAI
                     LRSSEHARAGLEILSGYPVPPEVEFYFGTSPAALLQLDLARITPERPEVSAGLFREVG
                     SKHWGVDLDYEPGYLPLIEELLMAALEGDEAHNNPPLSGGLVAGLGCFLGEIIRRNIN
                     PPGMWRPTEDWGEGAVVEIGDVVLDPVGKARAFLEYGPEESLAFYAEYVLEQWDGGEF
                     PTDAQA"
     gene            8115..8741
                     /locus_tag="VFQ10_00500"
     CDS             8115..8741
                     /locus_tag="VFQ10_00500"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9963845.1"
                     /translation="MRHGQSTANARGIWQGQMEFPLSKEGRVQACLAGRGLSGEPFEG
                     LYSSPLSRAFETAQIIRDRAGFEGEVVPVDGLSERNGGILEGHTWAEQEQLNPELAKK
                     FLAIPEEERWALVGAETDEEVIARFEEALSSIRALHPGGSRIVVVSHGGVMRAFLRER
                     FGTGVLPGTQRAANASITRMQWDESGPRLLEVASTAHLTEDPGSSTVE"
     gene            complement(8767..9396)
                     /locus_tag="VFQ10_00505"
     CDS             complement(8767..9396)
                     /locus_tag="VFQ10_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET9963846.1"
                     /translation="MADSRKRIALKPHMDRIREWVETGKTDEWIADALGTSASSVQSF
                     RSRNDIYRRERGLREPETVFEGVLDHGERDGWGLWLDPSIAEDPIWKEHWAGVEAVVV
                     KVAEDSIVLEADGGEMRSADDSAPSVATADGTFDEESSGLPRERGRVKWFDAEKGYGF
                     LVRPTGDDLFVHHSEVQGDPSELSPSGEVEYEVGHNDRGPNARSVRPLG"
     gene            complement(9579..10400)
                     /locus_tag="VFQ10_00510"
     CDS             complement(9579..10400)
                     /locus_tag="VFQ10_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963847.1"
                     /translation="MVELAEERSAAAWKIKNLQDYDGRLERAARRIDEISRERPEPAL
                     MIYASALKKSGRSEDEIRSMVREFRETFEDEEEPLTITRASAIMSVEGDGWFFGEENL
                     RVLTGRLLGQFQHRVAQYNYVDEREVLRRWADGYDTRLFVRRRIHETEPVVGVVGGLD
                     LTLLQYLRVAAGGNTLVPTENVSRALGALGYQGDAYETLAHAEGLALYLELPAPIVGE
                     MLEDLGREGFEDFPELPSETAGTEEQEEGLPSEGLPSEGDSVEGGAAEEKPSPED"
     gene            complement(10466..10681)
                     /locus_tag="VFQ10_00515"
     CDS             complement(10466..10681)
                     /locus_tag="VFQ10_00515"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9963848.1"
                     /translation="MDFKAAAREVLEEVGHPLHYTDITEIALQSGYLQSAGRTPQNTM
                     RARLSVDVRDNPATLFVQVAPGVYGLK"
     gene            complement(10696..11838)
                     /gene="pheA"
                     /locus_tag="VFQ10_00520"
     CDS             complement(10696..11838)
                     /gene="pheA"
                     /locus_tag="VFQ10_00520"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET9963849.1"
                     /translation="MTQSNLPDREDARLAESKGAPQGKMEALRQGIDSVDEEIVRLLD
                     RRAWLARRIGEIKHKNGLAAYSPARERAVLDRVATLGDGEFPRRGLQAVFREIISSSI
                     SLEARLKVAYLGPEATFTHEAALRSFGTSIELEPQTTVAEVFARVERGEVEHGVVPVE
                     NSMEGAVTHTLDELMNSPLKICGEVYLPVSQNLISAEQSMERVSLVCSHPMALAQAAS
                     WLRRELPGARLDEVDSTAEAARRAAVEPGVAAIGSVLAAGVHGLTVLARNIQDARANT
                     TRFIVLGRTWAARTGKDKTSVVFSVKDRPGVLRDALSAFAKEGINLTRIESRPSRKRA
                     WTYVFFADFLGHPQEDRVKRALEALEEHCPYVGLIGAYSEVSPDVA"
     gene            complement(12077..>12401)
                     /locus_tag="VFQ10_00525"
     CDS             complement(12077..>12401)
                     /locus_tag="VFQ10_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET9963850.1"
                     /translation="PGVSRSGATITLGLFLGLRREEAARFSFLMSVPIIAGAGGLQLA
                     EVITSGMSTHELLLFVTGFVSSAVVGYFTIRFLLHYLTGHSLRAFAYYRFAVAAVVAL
                     LLIVF"
BASE COUNT         2295 a         4140 c         3791 g         2175 t
ORIGIN      
        1 gatgggccct gtgatcgaag tacccccacg cttctcaagc ggtacgcgat cggccatcag
       61 ctttcagctt tgtcttggct gaatgccaac cgccgatggc tgatagctcc tgaagtgtga
      121 ggccaatgcc ttatgcacgg cgctgccagt caagataata ggtacacgca cttagcgtgt
      181 tattcgacca tcgcgggttt cgtctgtggt gcgggaagat gctcgagaga gtatcgcgta
      241 cggtcgtcaa cgcgctcacg ttggcaggaa agctcctgcc atggagcatg cgagccggcc
      301 gctcgggagg cagaacccag aacagggcta ccttcgagag ggggaggttt ctagctgggc
      361 cggcacgcta gtcctgctcc atcgcgcgtc cctcggagac ttccagggcg tagcccacgg
      421 cctgatccag ggtcatggat cttcccttct cccactcttc ctgccagacg gcctcctcaa
      481 gccgggagcg cgccgcatct atgcgcggct cgatgattgc gcgctcctct agtagccagg
      541 gagccaccag tagcctcgcg ataccccgct accccgcccc acggccgcgc tgccctgcgg
      601 atccctcccc tcgcagcagc gacgcttgca aggacgtcca agacgaaatc catacccggt
      661 gtttgggcct gctcctgtag ttctcttatg gcctccacgc agagttcctc agcccggtcg
      721 ggatcgttcc gggagagcgc caggaacccg aggcggctat tggcgagagg ggccatgccc
      781 ctatctccga cctctcggaa cagttcctgg gactcccgga ccaacgcctc cgcacgctcg
      841 tactcgccgg ttatcgccgc cgcgaacccg gtgttgtagg tcaagatggc gatgatttga
      901 acgtcactgg cctccctggc caggctcagg ctctcctcct gcatggcgga agcacgcgcg
      961 tagtcgccgc gataaacgtt caacatggcg agcgcgttga gcgctacgct cacgtccctt
     1021 gcggtgcccg ctgcccggct cagggcgagg ctctcctcca gcaacgcctc ggctctctgg
     1081 agatcactcc ggtccaaagc tatccatccg agaaagcaga gcgcataggc ctcacccctc
     1141 ctgtccccga cctcccgata cagcgaaagg ctcgcttcga ggtcctccac cgcccgcacg
     1201 taggcgccct gcctcagctc caggtacccc agattaagcg acgcctcgcc cctgacgcca
     1261 acaggagcgg cccatccccc tgccagacct gttcccagcc agtgccgacc ctcgctaaag
     1321 tggcccctga cgttccagaa gcgccacagg gcgccccgat ccgcagcgcc gactccgcgt
     1381 ccccagcctc cagcgatcgc gagagcgccg ccctgaagtt gtcgtgctcg gcctcaagcc
     1441 ggtccatcca cgacacctga tccgcacccg tcaactcggg cgcggcctct tccgcaacag
     1501 ccaggaagta tcgtattgtg tgcgcgcccg atctcctcgg cctccccgct atcttggagt
     1561 ctctcgcgag cgtactcgtg gatcgtctcg agcatcacga accgcggctc gccctccgac
     1621 ccttcctcct gccttaagag gctcatgtcg aggagggagg agacactctc gaaggcgtcc
     1681 aagggcagat ctccccccga gtcgcacact gcctctatag cgtcaagcgt cctgccaccg
     1741 gagaagacgg ccatccgcgc gaacagcgtc ttctcgcctt cgtcgagcag cgcgtggctc
     1801 cactctattg cgcccctcag ggtcctctgc ctctcgggca ggtcgcgcgc tcccccggtc
     1861 aggagcttca ggcgccgccc gagcctgcct agcatggccc tgggcggaag gagcttgacc
     1921 ctcgccgcgg ctagctctat ggcgagcggc aaaccgtcga gtctcgcgca gatctcggcg
     1981 accgcggggg cgttctcgct ggtgaccgca aagtccgcct tcgcggccgt cgccctctct
     2041 atgaagagcc tgaccgcctc gtagtgggag agggcctcga gaccgggcaa cctcttcgtg
     2101 tccggacact caggggcggc accgtgtact cgtgctcgcc gtagaccccg agcgggatgc
     2161 ggctggtggc gagcaccttg acctgcggtg cggacagcaa ctcgcccgac atcggggcag
     2221 cttccagcac ctgctcgaag ttgtctagca ctaccagcag gcgtttgtcc ttgaggtagt
     2281 ccctcacgcc ctcagtgagc ggtcgatctc cgctctccct gacgccgagt gcttgcgcga
     2341 cggtcgggac taccaacgca gggtcgctga tggtggccag cgccacgaag aagacgccgt
     2401 cctcgaactc ctccaggagg tccgcggcgg cctgcagggc cagccgggtc ttgcccgtgc
     2461 ccccgggacc ggtgagggta aggaggcgca cttccggggc aagaagccgc ccccggacct
     2521 ccccaacctc cctctcgcgc ccgacgagca gggtcggctg catgggcagg ttgttcaggc
     2581 ggctctcgag cgtcctcaat ggagggaaac cggagggcaa atccggcgcc aggagttcga
     2641 agacgcgctc gggacgggcg aggtccttta ggcggtggtc ccccagatcc cgcagttgtg
     2701 cgtccaccgg cagttgatca cgcaccagct cctgggccgg caaagagagg agcgtctgac
     2761 ccccgtgggc cgcagacaga agcccggttc aggggagggc cgaagtagtc cccgtccctc
     2821 tcctcggcgc tccccgtgtg aagcgccata cgcaccagcg ggggtccggc ctcgatccag
     2881 tctctatcta ccagcagtgc ccgctgggcg gcgagggcta catccagggc ctcaggagcg
     2941 gtgggaaacg cggcgcagaa ggcatctccc accgtcttga agacgtggcc cccgtgggcc
     3001 tctatgacgc tgcgcaggat ttcgtcgtgg cgggccaggg cggcgcgcat cgtcgccggg
     3061 ctctcctccc acatcctgct cgagccctct atgtcggtga acaggaaggt caccgtgccg
     3121 ctgggcggag cgaccacgtt gggactccct tttcctaggc gggcgtctct tcaactggag
     3181 ctatagatgc actacagaaa gcggcgcaag gctccccggt caggagacag gctccgtcac
     3241 gacgggacgt cccttcagga gaaaatacag cccggcgagc acgacggccc ctccgacgat
     3301 cgtcaggagg cccggccttt cggagagcac gagccaggcc aggagcgctg cgacgacggg
     3361 ctcggccagg atggtgcccg agatgatgga ggcctgcacg tagggcaggg cccagttgaa
     3421 gaccgtatgg cccatgagct gaggcccagc ggtgatcgcc gccagccaga accaggtttc
     3481 tccggagtaa ccccagagac ggacgtcgaa gctgagggcg aagggcagca acgccgccgc
     3541 tgccgccgag tacacgacga tggaataggg gagcacaccg acgcccccgg tggtgcggga
     3601 ggagcgaccg atcaggacgt acacggccac cgtcacggcc ccgacgaggg cgagcgcgtt
     3661 tcccagcacg gcggccgatc ctacggtcct gtcgaaggcg atgaaggccg tgcccgctat
     3721 ggcgaccacg atgccgagga aagatagcgg ggtggtgcgc tctccgaaga gtaggtatgc
     3781 cagtacagcc acgaagacgg gctgggtgga cacgaggacg acactcgcgg ccacgctcgt
     3841 atagtcgagc gaggagatcc agaacccgaa atgggcccct agcgccacgc ccgaggctat
     3901 ccccacgtag agtgcacggc ccctcgggaa gccgtccttc ctcacgagcg ccggcggcag
     3961 gagcacggcg gccccaagtg cacacctcca gaacgcgatg gcaatggcag gggcctcggc
     4021 cagccggata aagatggcgg acgccgatac tgcgaacacc cctacggcta cggcagggag
     4081 agcaacccta ccccatgcct tatcctgtac caggccagag ccccactttc cctgactaac
     4141 tgccccctac gggaccactt cttgcgccac ataggactac tatacaccgg ctccgccaca
     4201 gcgagcaggc caaccctgcc aaaggaggcg acagtcctga cgcagtgcaa tgggtccgtc
     4261 accacaagaa cctccccgat acctctgtgg tgtgcgatct cagccaccct cacggcggat
     4321 tcccaggtgt tccttgcggt atcctcgcgc aggatcgccg cctctggtac acccatctcc
     4381 cgcaggatgt ccgacatcac gacagcctca ccgggcggat gttcgccctc accgccggtc
     4441 ggtaccagta gatcagccag gccccgaccg tacaactcgc ccgcgtgacg cgtcctgacc
     4501 tccagcgtag gactcggtct cccacccggc aatacttgcg ccccgagaac cacgacgacc
     4561 cggggtctgg cccttttgcc gtcaggggct gaggtcgatg gccgacccac atacaccctc
     4621 aagagctgcc gtagcagccc gaggatgcct ccccgaaacg gacccttcaa cgccgggcct
     4681 cgcgccttgc cagcgccaga tcccctggcg agttgacgtt catcagcagg aggccggggt
     4741 ccccgaaccg ccttaactcc tccccgacgt actcgacctc atcaacccct tcgataaacc
     4801 cttgcaccgc ccgcgccccg ccgtcgagcg ccgtcccgag acgtggcagc agcgtcctgg
     4861 cgtaggcggc gcagagcggg tggattctgc tcccgtatcg ggggaccgca gcaagaacgc
     4921 ggcccctggc cagacgcccg agcaggtaac ttaccatgct ctgcgtcaag aagggcatgt
     4981 caccggctgc gacgaaggtt agcgggtaac gtgcggaggc gaagccagcc tccataccgg
     5041 acagcggtcc aagtccgcca ggtcgctcgc cggtgatgta tctgactcct tcgagtctga
     5101 caccgccatc tccgactacg agcacctcct ggcagcatcc tgtgagcgcg tctctgacac
     5161 gttctatcag gggcaccccc tcgacctcga gggatagctt ctcgcgtccc atcctgcggc
     5221 tggcgccgcc ggccaggatt atgcccgatg ccgggcggct caggtgaccg cccggacggt
     5281 cgctccctct ctcaagctga ggatcttttg gcggagcatc ctgacgcggt ccatgcttcc
     5341 agggctactc gtacgtcggc ccaggcgttt tgcgagggct aggtcaacgg cggcggcgtg
     5401 cagtttgcgg accctggacc atgtgcgcag tcttcccgta aagagtagcc caaggtagta
     5461 gcccgtcctt cggaagtagc tggggagtat ggcgtattct tgcgaggtta tcgtcccggc
     5521 ccggacctca tcactgagtt cgtcacgaat agcgcgccgc tcgatggcga gccacacgat
     5581 aatgatcacg atgtaaacga ggattacgca gaacaatacc aggtagccca cgggcccgaa
     5641 caccgtggcc gtaaagttga aggcagcgtg caacagtatc gccccaaaga ggccgaggac
     5701 gggcaggatg accttgagaa ccctgttgtc caccaagggg atgaggccga taccgatccc
     5761 cgtaagcgag gtgaaagacg cgtgggcgaa gccgccgaag atcctgcgga cgatatacgt
     5821 ctcaggaccg aactctagac cgtagagcag gtcctcagca atggagaagc cgaacccaac
     5881 ggccgagccg tagacgatcc cgtccatcac accggcgaac tccaccaacc cgctcctgcg
     5941 gcgcgcgagg taggcgatga agaagatcaa gagcagagcg gcccccttgg agcactcctc
     6001 caccacgggc gcctcaacaa ccgctgtgaa aaagctggcg gtcttcacgc tcgtaacgga
     6061 gctgagcgtg agctggaaga gcgtgttgaa gaccagtgag actgtcgtag ccaccgcgaa
     6121 cccccaaatg aagacaggga taacgtagcg cagcggctcc cgctcgtaga ggtctatcgt
     6181 ccggataaag aggaggtaga tcacggtttg caggatgccg agccagatca gtggtccgct
     6241 caggctcacg aggctgacct tccggagtat gacatggacc gcagtatacc cgctcctgat
     6301 aaaatacctc tgtccttacc gaaaggagag gattggacag ggacctcaga gaccgcgact
     6361 ctatactggc tgccctacgt cacctgaccc acggggtcta cgtgatgggg acccggcgtg
     6421 gccggcagtc caacgccatg acggcgtcgt gggtgatgca gacctctgaa cgtcccccat
     6481 gcctggcggt cgccgtgagg agcgatcgtt acacgcacga catcgccctg gagagccaaa
     6541 ccttcgccct tagcgtcctg cgcgaggatc aggtagacgt ggcgacccac ttcgcggaca
     6601 ccagcggcga gtaccacgac aagctccagg gcatccccta cggtcttacg ccggggggct
     6661 cgcccttcct cctcgactgc ctggcctacc tcgactgcaa ggtcatagac agggcccgtg
     6721 cgggcgacca caccgtggtc gtcggcgagg tcacggccag cgacaccctg gacgagagct
     6781 atcccctgat ctacgacccc ggcgagtacg aagaggccct caggtagacg gactcgaagc
     6841 ggcgtaagat ccaacagctc cgggtacaat gtgttcgaga gaacgaattc atgatcagga
     6901 gacgtgctat ggatgcgacc ctagagaaga caggcataag gctatttacg ggcgactact
     6961 gcccgttctg cagccgggtg agaaacgagc tggagcggct cgggctcgac tacgaggtcg
     7021 ttgacgccga cgcggacatg cgtgaggagg tcataaggct ctcgggacag cgggcgatcc
     7081 cgatcctcac catcggcgac gaggtcctcg tcgactcctc gcacataatc cgcgagcttc
     7141 gcaaccgcta cgcctgagcg tcagtgggga actcaccccc gtcccactgt tcgaggacgt
     7201 actcagcgta gaaagccagg gactcctcgg gcccgtattc gaggaaggcc ctggctttac
     7261 ccacggggtc gaggacgacg tccccgatct cgacaacagc tccttctccc caatcctcgg
     7321 tcgggcgcca cattccgggt ggattgatgt tgcggcggat gatctcgccc agaaaacatc
     7381 caaggccggc cacgaggccg ccggagagcg gcgggttgtt gtgggcttcg tcgccttcga
     7441 gggcggccat gagtagctcc tcgatgagcg gcaggtaccc cggctcgtag tcgaggtcga
     7501 cgccccagtg cttgctcccg acttcccgga agaggcctgc gctcacctcg ggccgttcag
     7561 gtgtgatccg cgcgaggtcg agctgcaaaa gcgcagccgg cgacgtgccg aagtagaact
     7621 cgacctcggg gggcacaggg tagccgctaa gtatctccag tcccgccctg gcatgctccg
     7681 aactccgcag aatagcggcc ttatcaagtg cctcgccgga cattcggtcg tcccatggcc
     7741 ccgtcgcgac ctcgacaaag atatcccttt cgtcctggcg caggtatatg ggaaggacca
     7801 cccgtccgag gggagactcg atctctttga agagctcgat gatcctgccc ccccgttctt
     7861 ccaactctcc ggccaccctg agaatggtgg ccggacgagg ggcgtcacgc tcgacctgta
     7921 tgggatctgc tttctcttcc ggagctgctt ctaggccatc tacttcgggc gtctcttctc
     7981 cgtctctcca gatcatcccg gcaagcctcc ccatcgaacc acgaacccgc agattctagc
     8041 atcggcgaag atttgccgga agcgcctcta tacactaccc ttacccgatg ccggaactac
     8101 aagagttgct cctggtgcgt cacggacagt ccacggcgaa cgctagaggc atctggcagg
     8161 gccagatgga gttcccactc tcaaaggaag ggagagtgca ggcgtgcctt gcaggacgcg
     8221 ggctctctgg ggagccgttc gaaggcctct attcgagccc gctctctcgc gccttcgaga
     8281 ccgcacagat catccgcgac cgtgcgggat tcgaaggcga ggtcgtcccc gtcgacggcc
     8341 tgagcgagag gaatggaggc atcctcgaag gccacacctg ggccgagcag gagcagctca
     8401 accccgagct ggcaaaaaag ttcctcgcga tccccgagga ggaacgctgg gcgctcgtgg
     8461 gcgccgagac ggacgaggag gtcatcgcga ggttcgaaga agcactctcg tccatccgcg
     8521 ctctccaccc aggcggttcg cgcatcgtcg tcgtctcgca cggaggggta atgcgggcgt
     8581 ttctgaggga acgcttcggg accggggtac tgcccggcac gcaacgggcg gcgaacgctt
     8641 cgatcacacg catgcaatgg gacgaatcgg gcccgcgttt gctcgaggtg gcatctacgg
     8701 cgcatctaac cgaagatcca ggctcctcta cagtagagta gggggaggtt tgaaaccctc
     8761 cctactctag cccagaggcc tgacgcttcg cgcgttcggg ccccggtcgt tatgacctac
     8821 ttcgtactct acctctccgc tgggagagag ttccgaagga tcaccctgta cctcagaatg
     8881 atggacgaag agatcatcgc ccgtgggtcg caccaggaaa ccgtaacctt tctctgcgtc
     8941 gaaccacttg acgcgccccc gctcgcgggg caacccggac gactcttcat cgaacgtccc
     9001 gtcagcggta gcaacagatg gcgcggagtc gtctgcggac ctcatctctc ccccgtcggc
     9061 ctcgagcact atggagtctt cggctacctt tacgacgacg gcctccaccc ctgcccagtg
     9121 ctccttccag atcgggtcct cagcgatgga agggtcgagc cacaggcccc agccgtcgcg
     9181 ttcgccgtgg tcgaggactc cttcgaagac ggtctcgggc tcgcgtagcc cgcgctcgcg
     9241 ccggtagatg tcgttgcgcg agcggaagga ctgtaccgag gatgcgctgg tgcccagggc
     9301 gtctgctatc cattcgtccg tcttgcccgt ctccacccac tccctgatac ggtccatgtg
     9361 aggtttcaag gctatgcgct ttctggagtc tgccatgcta ccgcctgctc cttcttcgaa
     9421 cccgttctga tagatgctgc gctgagcggt tgttagacga gttattgttg gagatacctc
     9481 tgtgcattct accatttctc cgggcttgcg ggcagcctcg ggtggggagg ttttcttggc
     9541 gggagcatct gtgacccgcg ggccatctgg cagggtctct agtcctcggg actcggcttc
     9601 tcctccgccg ctccaccctc tacggagtct ccctccgatg ggaggccctc cgatgggagg
     9661 ccctcctctt gttcctcagt ccctgctgtc tcggacggca gttcgggaaa gtcttcgaaa
     9721 ccttcgcggc cgaggtcttc cagcatctcc ccgacgatgg gagcgggaag ctcgaggtac
     9781 agggcgagac cctccgcgtg ggcgagggtc tcgtatgcgt ccccctggta gccgagggca
     9841 ccgagcgccc tggagacgtt ctcggtaggc acgagggtgt tgccgccggc cgccacgcgc
     9901 aggtactgga ggagcgtaag atccaagccc cccaccacgc cgacaacagg ctcggtctcg
     9961 tggatgcgcc gcctcacgaa gaggcgtgta tcgtagccat cggcccagcg tctcaagacc
    10021 tcgcgctcgt ccacgtagtt gtactgggcc accctgtgct ggaactgccc tagcagcctg
    10081 cctgtaagga cgcggaggtt ctcctccccg aagaaccagc cgtcgccctc gacgctcata
    10141 atggcgctcg cacgggtgat ggtcagcggc tcttcctcgt cctcgaatgt ctcgcggaac
    10201 tcgcggacca tgctgcggat ctcatcctca ctacgtccgc tcttcttgag tgcgctcgcg
    10261 tagatcatca gcgcgggttc agggcgctcc ctggatatct cgtcgatcct gcgcgccgcc
    10321 ctctcgagcc gcccgtcgta gtcctggagg ttcttgatct tccaggccgc cgcgcttcgc
    10381 tcttccgcta actcgaccat ggtctctcct cgggtctcga agaatggtac ctcgcaatat
    10441 agcatctccc tttgcgtcag gacgatcatt tcaggccgta gacgccgggc gcgacctgca
    10501 cgaatagagt agccggattg tccctgacgt ccacggagag cctcgccctc atcgtattct
    10561 ggggcgtgcg gcccgcgctc tggaggtacc ctgactgcag tgcgatctcc gttatgtccg
    10621 tgtagtggag agggtgaccg acttcctcga ggacctcccg cgcggcagcc ttgaaatcca
    10681 cgcagtctca ggccctcagg ctacatcggg agagacttcg gagtatgccc cgatcaggcc
    10741 tacgtaaggg cagtgctcct cgagggcttc tagggcgcgc ttcaccctgt cctcctgggg
    10801 atgaccgagg aaatccgcga agaacacgta ggtccaggca cgcttgcggc tggggcggct
    10861 ctcgatacgg gtaaggttga tcccctcctt cgcgaaagcc gagagcgcgt ccctgagaac
    10921 gcctgggcga tccttgacgg agaatacgac gctggtcttg tccttgcccg tacgcgcggc
    10981 ccacgtgcgc ccgagcacga taaagcgcgt ggtgttcgcg cgggcgtcct ggatgttgcg
    11041 tgccaggact gttagaccgt ggacgccggc cgccagcacg cttccgatgg cggccacccc
    11101 cggctcgacg gccgcccgcc gggcggcctc cgccgtggaa tcgacctcat ccaggcgtgc
    11161 tccagggagc tcacgcctga gccagctcgc ggcctgggca agggccatgg gatgcgagca
    11221 caccagactc accctctcca tcgactgttc tgcggagatc aaattctggg agacgggcag
    11281 gtaaacttcc ccgcaaatct tcagtgggct gttcatcagc tcgtccaggg tgtgggtcac
    11341 ggcgccctcc atggagttct cgacgggcac gacgccgtgt tcgacctccc cacgctcgac
    11401 cctggcgaag acctcggcga ccgtcgtctg aggctcgagc tcgatactgg tgccgaacga
    11461 acgcagggcg gcctcgtggg tgaacgtcgc ctcgggcccg aggtaagcga cctttaggcg
    11521 ggcctcgagc gagatggagg atgagatgat ctcgcggaaa acagcctgga gtccacgcct
    11581 ggggaactcc ccatcgccaa gcgttgcgac acggtcgaga acggcccgct ctctggcagg
    11641 ggagtaggcc gcaaggccgt ttttgtgctt gatctcacca atcctccgcg ccagccaggc
    11701 acgcctgtcg agcaagcgca cgatctcctc gtcaaccgag tctatcccct ggcgcaatgc
    11761 ctccatcttg ccctgcggcg cccctttgga ttcagcgagt cgagcgtctt ctctatctgg
    11821 taagttggac tgagtcatga cgtgtgcgat ctcctgtcga aatgttgtca ggatgcgggt
    11881 ttggaggagc gcggtcgcgc gtcaggaaag ggagcgcccc tcccgggtaa atcgccgcaa
    11941 tgcacaggcc gcctggcgca tggtgagtca aagtaacaga tagcggcccc cgctgtcaaa
    12001 ggtccaagca tttcagcacg ccgccaggct tcgcaggacg gcttgtagta aggcgatacc
    12061 cttgcgcatc gtttagttag aacacgataa gcaagagggc gacgacggcc gcgacggcaa
    12121 agcggtagta agcgaaagca cgcaggctgt gcccagtaag gtagtggagc aggaaccgta
    12181 ttgtgaaata ccctacgact gccgagctaa cgaagcctgt cacgaacagc agcaattcgt
    12241 gcgtgctcat gccggaggtg atcacctccg caagctgcag gcctccggcg ccggcgatga
    12301 taggaacgct catcaggaac gagaacctgg ccgcctcctc gcgcctgagc cccagaaaga
    12361 ggccgagtgt gatcgtggcc cccgaacgcg agacgccggg c
//
LOCUS       DASTKM010000015         6918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_354856, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000015 DASTKM010000000
VERSION     DASTKM010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354856"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFQ10_00530"
     CDS             complement(<1..616)
                     /locus_tag="VFQ10_00530"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HET9963851.1"
                     /translation="MREDLQEIFSAGLAAADPQRAVRRELELEGDAILAREKRFEPRR
                     VYVLAAGKAAGEMAKAAEELLGEKVSGGLVVTKDRHDPGPEGFETVFASHPEPDERSV
                     EAARRVQELAESLGEEDLLLALISGGASALLADPAPPIELADLKKLTSDLLKSGADIG
                     EINTVRKHVSVLKGGGLTRLAHPAPTIALLLSDVVGDEPSSIASG"
     gene            686..1501
                     /locus_tag="VFQ10_00535"
     CDS             686..1501
                     /locus_tag="VFQ10_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET9963852.1"
                     /translation="MKFSANVSILFKEVPLLERFGLAAAAGFSAVEFWWPGHEAEPGT
                     VERAAQDAGLDVALFNFDAGDMAAGDRGLVSDPERQQLFRENVPIALDLARALGCERM
                     NVLVGHEIPGLDREEQLALARENVRFAAEKAEAAGVTVMVEAVNTFENGPYLLYTTEQ
                     AVEFVERVGRENVRIQHDFYHMQRMEGNLVANLREHFDHIGHVQIADSPGRGEPGTGE
                     IHYPYVLAALEGLGYDGYVGLEYNPTTERTEESLKWLPKEVRGRDVGVSDLGF"
     gene            1513..2406
                     /locus_tag="VFQ10_00540"
     CDS             1513..2406
                     /locus_tag="VFQ10_00540"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HET9963853.1"
                     /translation="MAEKVGFIGLGIMGRPMAKNLMEAGYELVVHNRSPEKAEELAKE
                     GNATAAASPGQVAQACDIVITMLPDSPDVEAVVAGEGGVLEGIRDGALLVDMSTISPV
                     VTKELSEKVREKGASMLDAPVSGGDVGAIEGALSIMVGGSEEDFERARPLFDVMGKAA
                     THVGPIGAGQVVKACNQIVVALTIEAVSEALVLGSKGGVKPEKLVEALSGGLAGSAVM
                     EAKKEKFFSHDFEPGFRIELHHKDLGIALAAGREYGVALPATAIVDQMLEALKAKGRG
                     DRDHSALLTLLEEAAQHEIGE"
     gene            2438..3892
                     /locus_tag="VFQ10_00545"
     CDS             2438..3892
                     /locus_tag="VFQ10_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET9963854.1"
                     /translation="MERSRLIRKMESIVGAEGVVHEHEQLRTYECDGLMNYRVIPDLV
                     VLPETGEHVQKVVKLCYDEGIPFVARGSGTGLSGGALPVESGVLIVLSRMRKILEVDI
                     PNQRIVVEPGVVNMWVSQEVADEGYYYAPDPASQIVSSIGGNLAENSGGVHCLKYGFT
                     TNHVMGAEVVLPDGSVTHIGGRRAPDAPGYDLLGAFVGSEGTLGIATRITLRLCRVPE
                     AVRTLLAGFEDTEGAGGAVSGIIGEGIIPAAIEMMDRLTMEAVEAAVHPGLPDAGAVL
                     VIELDGPEAEVEEQFGRVLEICEENEATEIRVAVDDDERALFWKARKAAFGAMGRISN
                     DYYVQDGVIPRTELPAVLGKISELAGRYDLRVANVFHAGDGNLHPLVLYNNDAEGEAE
                     RAEKLAGEIVFACLDHGGSLTGEHGIGEDKKKYMPRMFTDDDMNVMQLLRCAFDPHQI
                     SNPGKIFPTPRLCGERPGPFHMHPLQRAGIVEQF"
     gene            3892..5163
                     /locus_tag="VFQ10_00550"
     CDS             3892..5163
                     /locus_tag="VFQ10_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9963855.1"
                     /translation="MQTEKGLAEDLRRIVGHDNVHAATEEDAVEGVEPDFVVEPGSVE
                     EISDVMKLAASEGLAVAPRGSGTKTHIGDPPRRLDLIVSSARMNEVLEHTPGDQIVRV
                     EAGVKLEDLQEYISSSDQMLAIDPPEMGATIGGIVAANSSGPRRYRYGTIRDLIIGIT
                     VVLHDGTVAKAGGKVVKNVAGYDLSKLFTGSLGTLGIIATANFRLHPRPIAARTVGVE
                     VAGPQQAQAAAQTIVHSQVEATAIELHYGGDEKLLTVLLESIPGGIDAKTETASFLLK
                     QFGEVRTISDEEANQLGPLTTPQVADEVVLKIGAPPADLAAVLESVLGAAERKGLGHP
                     RITGHAGTGVTLVGFSGENEADAAGFVEEMRGIWVRRGGSVTLQRAPLTLKQRVSTWD
                     NGGDYLGLIRRVKEKFDPRGGMNPGRFLGGN"
     gene            5163..6689
                     /locus_tag="VFQ10_00555"
     CDS             5163..6689
                     /locus_tag="VFQ10_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET9963856.1"
                     /translation="MTERNGQGGAREAGDDRYDAMDATQRRSDEGTAREDAMVGAIGA
                     GSPEDVGQSTFAATTDSLQHTMGEDRPESGSYAFPAFDDHHPPDKALIDECVHCGFCL
                     PTCPTYVLFGEEMDSPRGRIYLMNKGLDEEPMNDEMVRHWDLCLGCMACVTACPSGVQ
                     YDKLIESTRQQVERRYERRADDRAFREMIFQFFPYPNRLRAIAAPMRLYQRFGIGDKL
                     RRAGIMQRIPARMRAMEALLPDLPREEKIPDLTGPVGEKRRRVGVLTGCVQRVFFSPV
                     NAATVRVLAAEGCEVVAPKQGCCGALSTHAGREEESLDFARQTIDTFEDLNLDNVIVN
                     AAGCGSTMKEYGYMLRDDPEYAERAKTFSAKVRDVSEFLQELGPVAERNPLPVTAAYH
                     DACHLAHAQGIRKQPRQTLKQIPGMDVKEIREAEICCGSAGIYNMVEPEPAAELGERK
                     ANNILKTGAQMIVTSNPGCMLQIQASLKKMGRNGLPMAHPVEVLDASLRGEPVETLLG
                     "
BASE COUNT         1333 a         2094 c         2369 g         1122 t
ORIGIN      
        1 ggccgctcgc gatagaagag ggctcgtcgc caaccacgtc ggagagcagg agggctatgg
       61 tcggggccgg atgggcgagc cgcgtgaggc cgccgccctt cagtacggag acgtgcttcc
      121 ttacggtgtt gatctcccct atatccgccc cgctcttgag gaggtcggac gtaagcttct
      181 tcaggtcagc aagctcgata ggaggagccg ggtcggcgag cagcgctgat gccccgcccg
      241 agatgagagc gagaagcagg tcttcttctc ccagggactc cgcaagctcc tgtactctgc
      301 gggccgcctc cacgcttctc tcgtcgggct cgggatggga ggcgaagacg gtttcgaagc
      361 cctcgggacc tggatcgtga cggtcctttg tgacgacgag cccgccggag accttctccc
      421 caagaagctc ctcagcggcc ttcgccatct ctccagccgc cttgcccgcc gcgagtacgt
      481 acacgcgccg cggctcgaaa cgcttctctc tggccaggat ggcgtcgcct tcgagctcta
      541 gctcgcgccg cacggcccgc tgcgggtcgg cggcggcgag accggccgaa aaaatctcct
      601 ggaggtcctc gcgcacaagg gcattgtaac catccgggag ttcggcgccc ggttgggtta
      661 tgcttgtggc ggaaggaggg tctgcatgaa attcagcgcg aacgtttcta tcctgttcaa
      721 ggaagtcccg ttactggagc ggttcgggct ggcggccgca gcaggcttct ctgcggtcga
      781 gttctggtgg cccggccacg aagcggagcc ggggacggta gagcgcgccg cccaagacgc
      841 cggactcgat gtggcgctct tcaacttcga cgccggagac atggcggccg gagacagggg
      901 gctggtgagc gatcccgagc ggcagcaact tttcagggag aacgtgccaa tcgccctcga
      961 tctggcgcgt gcgctgggct gcgagaggat gaacgtgctc gtggggcatg agatccccgg
     1021 cctggaccgc gaagagcagc tcgcgctggc acgcgagaac gtacgcttcg ccgcagagaa
     1081 ggcagaagcg gcgggtgtca cggtcatggt cgaggcggtc aacaccttcg agaacgggcc
     1141 ttacctcttg tacaccacgg agcaggccgt ggagttcgtg gagcgtgtcg ggcgggagaa
     1201 cgtgcggata cagcacgact tctatcacat gcagcgcatg gaggggaacc tggtcgccaa
     1261 cctgcgcgag cacttcgacc acatcgggca cgtccagatc gcggattctc cggggcgtgg
     1321 ggagccgggg acgggtgaga tccactaccc ttacgtcctg gccgccctcg aaggtctcgg
     1381 ctatgacggc tatgtcggac tggaatacaa cccgacgacg gagagaaccg aggagagtct
     1441 gaaatggctg cccaaggaag tgcgaggccg ggacgtgggc gtatctgacc tgggatttta
     1501 gagaggagca acatggctga gaaggtaggc ttcatagggc ttgggatcat gggccggccg
     1561 atggcgaaga acctgatgga agcaggctac gagttggtcg tccacaatcg cagccctgag
     1621 aaggcggaag aactggcaaa ggaggggaac gctacagccg cggcgagccc ggggcaagtg
     1681 gcacaggcgt gcgacatcgt catcaccatg ctccccgact cgccggacgt ggaagccgta
     1741 gtggccgggg agggcggcgt cctcgaaggc atccgcgacg gcgcgctgct ggtagacatg
     1801 agcacgatct cgccggtagt caccaaggag ctttcggaaa aggtccgaga aaagggcgcc
     1861 tcgatgctgg atgctccggt ctccggaggc gacgtggggg ccatagaggg cgcactctcc
     1921 atcatggtgg gcggttcgga ggaggacttc gagcgcgctc gcccactgtt cgacgtgatg
     1981 ggcaaggcgg cgacgcatgt gggacccatc ggagccggtc aggtggtcaa ggcgtgcaat
     2041 cagatcgtgg tcgcgctcac catcgaggct gtctccgagg cgctcgtgtt aggctccaag
     2101 ggcggggtta agccagagaa gctggtggag gccctctccg gggggctggc gggcagtgcg
     2161 gtgatggagg ccaagaagga gaagttcttc tcccatgact tcgagccggg tttcaggata
     2221 gaattgcacc acaaggatct gggtatagcc cttgcagcgg ggagggagta cggggttgcc
     2281 ctgccggcca cggccatcgt ggaccagatg ctcgaagcgt tgaaggccaa gggccggggc
     2341 gacagggatc actcggcgct actcaccctg ctggaggaag ccgcacaaca cgagataggg
     2401 gagtgatgct gaggcttccc acccaaggag ggtacacatg gagcgtagcc ggctcataag
     2461 gaagatggaa agcatcgtgg gtgcggaggg cgtggtccac gagcacgagc agctgcgcac
     2521 ctacgagtgc gacggcctta tgaactaccg cgtcatcccc gacctcgtcg tcctccctga
     2581 gaccggcgaa cacgtccaga aggtagtcaa gctctgctac gacgagggaa ttcctttcgt
     2641 cgcgcggggc tcgggaaccg gcctctcggg cggggctttg ccggtcgagt caggcgtttt
     2701 gatcgtgctc tccaggatgc gcaagattct ggaggtggac atcccgaacc agcgcatcgt
     2761 ggtcgagcct ggcgtcgtca acatgtgggt gagccaggag gtcgccgacg agggctacta
     2821 ctacgcccca gacccggcca gccagatcgt ctcctccatc ggcggcaacc tggccgagaa
     2881 ctccggcggc gtccactgcc tgaagtacgg ctttacaacg aaccacgtga tgggcgcgga
     2941 ggtcgtgctc cctgacggct cggtgacgca catcggtggc agacgggcgc cagatgcgcc
     3001 tggctacgac ctgctcggcg ctttcgtcgg ctcggagggg acgctgggta tcgccaccag
     3061 gatcacgctc cggctttgcc gcgtccctga ggcggtgcga accttgcttg cgggcttcga
     3121 agataccgag ggggcaggcg gtgcggtctc gggtatcatc ggcgagggga taatccccgc
     3181 cgccatcgag atgatggatc gcctgactat ggaggcggtc gaggcggccg tgcatccagg
     3241 cctgcccgac gccggcgcgg tgctcgtcat cgagctggat ggacctgaag ctgaggtaga
     3301 ggaacagttc gggcgggtgc tggagatctg cgaggagaac gaggccaccg agatccgggt
     3361 ggccgtggac gacgatgaac gcgccctctt ctggaaagcc cgcaaggccg ctttcggtgc
     3421 tatgggccgc atctccaacg actactacgt ccaggacggc gtcatcccgc gtacggagct
     3481 ccccgcggtg ctgggcaaga tctccgagct cgctggtagg tacgacctgc gggtcgccaa
     3541 cgtcttccac gccggggacg ggaacttgca tcctctcgtt ctctacaaca acgacgccga
     3601 gggcgaggcc gagagggccg agaagctggc cggcgagatc gtcttcgcct gcctggatca
     3661 cgggggatcg ctcaccgggg agcacggcat cggggaggac aagaagaagt acatgcccag
     3721 gatgttcacc gatgatgaca tgaacgttat gcagctcctg cggtgcgcgt tcgatccgca
     3781 tcagatatcc aacccgggca agatcttccc taccccccgg ctctgcggcg aacgtccagg
     3841 ccccttccac atgcatcccc tgcagagggc cggcatcgtg gagcagttct aatgcagacg
     3901 gagaaagggc tcgcagaaga cctgagacgc atagtgggcc atgacaacgt ccacgcagca
     3961 acggaagagg acgctgtcga aggcgtcgag cccgacttcg tggtcgagcc cggctccgtc
     4021 gaggagatca gcgacgtgat gaagctcgcc gccagcgagg gcctggccgt cgcgccacgg
     4081 ggcagcggca cgaagacgca catcggcgac ccgccgcgcc ggctcgacct catcgttagc
     4141 tccgcgcgca tgaacgaggt gctggagcac actccaggtg accagatcgt ccgggtcgag
     4201 gccggagtaa agctggaaga cctgcaggaa tacatctcca gctcggacca gatgctcgcc
     4261 atagacccac cggaaatggg ggccaccatc ggcggtatag tcgcagccaa ctcttcgggg
     4321 ccgagacggt acaggtacgg caccatccgc gacctgatca tcggtatcac cgtcgtcctg
     4381 cacgacggca ccgtggccaa agccggcggc aaggtcgtca agaacgtggc cggctatgac
     4441 ctctccaagc tcttcaccgg ctccctcggc accctgggta tcatcgccac cgccaacttc
     4501 cgcctccacc cgcgcccgat agcagcccgc accgtgggcg tagaggtggc aggaccccag
     4561 caggcacagg ctgcggcgca gactatagtt cattctcagg tcgaggccac agcgatcgag
     4621 ctgcactacg gcggggacga gaagctgctg acggtcttgt tggagagcat tcccggcggc
     4681 atagacgcca agacggagac cgcctccttc ctcctcaagc agttcgggga ggtgcggacg
     4741 atctcggacg aggaggcgaa tcaactcggt cccctgacga cgccccaggt cgccgatgag
     4801 gtcgtcctga aaataggggc tccgccggcg gatctggcag cggtgctcga atcggtcctt
     4861 ggcgcggcgg agcgcaaggg tctcggacat ccgcgcatca cgggtcacgc agggaccgga
     4921 gtaaccctcg tcgggttctc cggggagaac gaagctgatg cggccgggtt cgtggaggag
     4981 atgcgcggga tctgggttcg ccgcggcgga agcgtgaccc tgcaacgtgc tcctctaacc
     5041 ctgaagcaga gagtgagtac gtgggacaac gggggagact atctcggtct catccggagg
     5101 gtgaaggaga agttcgatcc ccggggcggc atgaaccccg gcaggttcct gggaggtaac
     5161 tagtgacgga gcgaaacggt cagggcggag cccgtgaagc aggcgacgac cgctacgacg
     5221 cgatggacgc aacgcagcgc cggagcgacg aggggacggc gcgcgaggac gctatggttg
     5281 gtgccatcgg cgccgggagt ccggaggacg tggggcagag taccttcgcg gccaccacgg
     5341 actctctgca gcacaccatg ggcgaggaca ggcccgagag cggcagctat gcgttcccgg
     5401 ccttcgacga ccaccatccg ccggacaagg ccctcattga cgagtgtgtc cactgtggct
     5461 tctgcctgcc gacgtgcccc acctacgtgc ttttcgggga agagatggac tccccgcgcg
     5521 ggcgcatcta cctgatgaac aagggcctcg acgaggagcc gatgaacgac gagatggtcc
     5581 gccactggga cctctgcctc gggtgcatgg cctgcgttac ggcctgccca tccggcgtgc
     5641 agtacgacaa gctcatcgag tccacgcgac agcaggtcga gcgccgctac gagcgccggg
     5701 ctgacgaccg ggccttcagg gagatgatct tccagttctt cccttatccc aaccgccttc
     5761 gggccatcgc agccccgatg cgcctctacc agcgcttcgg gatcggagac aagctgcgca
     5821 gggccggcat catgcaacgc ataccggcca ggatgcgggc tatggaagcg ctactgccgg
     5881 atctaccaag agaagagaag atccctgacc tcacaggccc tgtaggcgag aagcgtcgca
     5941 gggtcggtgt cctgacgggc tgcgtccagc gggtcttctt ctcgccagtc aacgccgcca
     6001 cggtccgcgt tctggccgcc gagggctgcg aggttgtggc ccccaagcag ggttgttgcg
     6061 gcgccctctc cacccacgcg ggccgcgagg aggagtccct agacttcgcc cgccagacca
     6121 tagacacctt cgaggacctg aacctggaca acgtcatcgt caacgccgca ggttgtggct
     6181 cgacgatgaa ggagtacggt tacatgctgc gcgacgaccc ggagtacgcg gagagggcga
     6241 agactttcag cgcgaaggtc cgcgatgtct cggagttcct tcaggaactc ggccccgtgg
     6301 ctgagcgtaa tccgctgcct gttacggcgg cctaccacga cgcctgccac ctggcgcacg
     6361 cgcagggaat ccgcaagcag ccgcgccaga cactcaagca gataccgggg atggacgtaa
     6421 aggagatccg tgaggccgag atctgctgcg ggtccgcggg catctacaac atggtcgagc
     6481 ccgagcccgc cgccgagctc ggtgagcgca aggcgaacaa tatcctgaag accggggcgc
     6541 agatgatcgt cacctccaac cccggttgca tgcttcagat acaagcctct ctgaagaaga
     6601 tgggccggaa cggccttccg atggcccacc ccgtggaggt cctagacgcc tcgctgcgcg
     6661 gagagcccgt agagacgctg ctcggttgat gtgggatccg ggtaggaatg tatgggagtc
     6721 gggagaaaga ggaacacgac tcccaaaccc accagggatg gaatccttct accggtttac
     6781 gactggagaa gctcctgctc gcttgtggta tgcgaaaacc ccggcgtgag catggctggg
     6841 aagatgatct ttggaccctg gtgaagtagc ggggtggccg cgcaaccgtt gggagctatc
     6901 cgcagcggag tcttacgc
//
LOCUS       DASTKM010000016         7812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_354879, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000016 DASTKM010000000
VERSION     DASTKM010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354879"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VFQ10_00560"
     CDS             <1..1143
                     /locus_tag="VFQ10_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963857.1"
                     /translation="RGDRDEKAWAFESAVEKALWWMLRSTLGRALRLMLRLWRGSKIF
                     RITSYTGAGILVTAGIAFTSVRIYENRHASIHVGPGAYELSLRGDRFSIYTAKGIEVW
                     NLDNRRRIGTVRDGDQTPDFMSPDGRLLAGGVQHGEYPEHTVEDIRIRRTEDGSLIRM
                     IDTAKDTGWPVWSPDGELIAALTPDAIQLVKVRDGRLLTSIEGYPPLAEDPVPIVWSP
                     DGRFLAMKGERGGIALWDVTSGRYLRTLEGDFESGISFSPDGRLFAESGTPVKLRSTT
                     DGSVVRKLRSGEPAGPVYASSVLAFSPGGELLVTGGADGRIRLWQVNDGKLLTEYSKH
                     AHNETDDTSSVLAVAFFPDGRHIASHGSDETVRVWPVEDYGLEGER"
     gene            1314..2363
                     /locus_tag="VFQ10_00565"
     CDS             1314..2363
                     /locus_tag="VFQ10_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET9963858.1"
                     /translation="MALNEALLKRLIETPGVSGREERQREIAREELGSLTDEVRTDAL
                     GSVIGTKKGSGDGRVMVAAHTDEIGYLVKHIDDKGFVRLQTLGGHDPANMVSQRVLVT
                     TADGETLRGALQPERKPPHVARGEEQKQPKAGEFYVDLGMDAETVKEKVRIGDFVVMD
                     RTLEKVGETYVGKAMDDRVGVFVMYEALRAMGDHQATIFAAATSQEEVGLRGAEASGS
                     ALEPTVVVALDVTLAMDVPGAGKENTITELGKGVAIKIMDGYSISHPKLVEHLRAIAE
                     RENIPYQMEILPAGGTDAGGVQRLHGGIPAVTLSIPCRYVHTPNEMVSATDVEAAITL
                     LARYLEETSDADYAL"
     gene            2367..3146
                     /locus_tag="VFQ10_00570"
     CDS             2367..3146
                     /locus_tag="VFQ10_00570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9963859.1"
                     /translation="MDKTLVIEENGAEFLMTLGRAAGAEERDDGRVRWAIGNSPIDYH
                     NCVVHADLTQNEADREIEASLQRMREHEVPGSWHVGPSMRPPDLGWRLITHGFEYGGD
                     DIGMAVDLSALPHEVPVPDDFVIERVRDEAGLAAWVEALGSGFGEGPVEAEWVGEMYR
                     RLGFEGPWRHYLGRVDEEPVATATSFFGAGVAGIYFVCTVERARRRGIGARITLAALS
                     EARDLGYSVGVLGSSEMGYSVYRGLGFEEYCRIGLYEWRPG"
     gene            3223..3744
                     /locus_tag="VFQ10_00575"
     CDS             3223..3744
                     /locus_tag="VFQ10_00575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9963860.1"
                     /translation="MANKTYDRPPEMALEPGYEYYANLVTEKGPVKVRLFAEEAPETV
                     NNFVFLARDGYFDGTTFHRVIVDFMVQGGDPTGTGTGGPGYRIPDEFHPGLRHDRPGI
                     LSMANAGPNTGGSQFFITHVATPWLDDKHAVFGEVVDGMEVVGAIRERDPQRDREPGD
                     RIERIEIEELPHS"
     gene            complement(3842..4465)
                     /gene="lepB"
                     /locus_tag="VFQ10_00580"
     CDS             complement(3842..4465)
                     /gene="lepB"
                     /locus_tag="VFQ10_00580"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9963861.1"
                     /translation="MSQIRPGALSYYFMSEKPYYHDQQFQTSSEERRPATKKGGGALE
                     YLVILLVSFALVFGFVRPFVMEAFWIPSGSMIPTLEIGDRVLVNKFIYRFTDPRRGDI
                     IVFESVDNSNEDLIKRVVGLPGDKIAVRGGKLFVNGEPQKEPYTNKKPPDRSFYARTT
                     VPKDHVFVMGDNRGNSADSRVFGPLPEKNIEGEAFLRFWPPERIGLL"
     gene            complement(4479..5249)
                     /locus_tag="VFQ10_00585"
     CDS             complement(4479..5249)
                     /locus_tag="VFQ10_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9963862.1"
                     /translation="MDYLEFLFLGVIGIVGGMLSGLVGVGGGIVFVPGLLCVGGWRIQ
                     DAVAASLVIIIFSSLSGTIRNARSADPVNWRVAGLLSLAVAPSSLIGVLISRVSPEVV
                     VKVAFATLLISLAYPTARGRGEYDSDRKNLPLPLVILAGIFVGALSGLVGVGGGAVMV
                     PLMVLGLGLTTKQAVSTSLAVVMLTGIVGAAGYVATGFREVQDLLSLPPLIIGSVIGA
                     PLGVRIRDWLPERLVRIGFGVFMVVVALHLLGEALGVF"
     gene            complement(5379..5993)
                     /locus_tag="VFQ10_00590"
     CDS             complement(5379..5993)
                     /locus_tag="VFQ10_00590"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET9963863.1"
                     /translation="MLEAILHPLIEFVTETIGSYGVPAVFVLMLLESMGILIPSEAIS
                     PFAGYLVSEGEMSFLAAVTAGVLGNVVGSWIAYFLGLWGGRELWFHYGRYVGVRAHHL
                     HIAEKWFDKYGEFTVFVSRCLPVVRTFISFPAGTARMNFARFTLYTFLGCIPWVFALT
                     YFGYVLGENWTTIGDYLHYLDYAVAMALVAGALYLFWRWRRSAS"
     gene            complement(6104..6823)
                     /locus_tag="VFQ10_00595"
     CDS             complement(6104..6823)
                     /locus_tag="VFQ10_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9963864.1"
                     /translation="MLEGRREIVRWVADRLTVVAAAWLAAGLTFSAFVIWAFTELADV
                     VVEGESRALDRAVLLWIHTTFPSWLDGPMRIVTALGYYWFVVLLLVVVVPLFYQRGWR
                     LSAILLLISTAGSAVLTTVLKSVFQRARPELFDSGYHASFYSFPSGHATVAVGLYGML
                     TLVLAYRLRGTARWAVVVSGILVVFLIGFSRLYLGVHYPTDIVAGYLAALLWLVCVGA
                     VYALWLSVRSLGAAESSRSDG"
     gene            6908..>7812
                     /locus_tag="VFQ10_00600"
     CDS             6908..>7812
                     /locus_tag="VFQ10_00600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9963865.1"
                     /translation="MQNNDIVRALETIATLMEIKDEAYYRVLGYQRAAESVAALGRPA
                     TEVDDLKTLPHVGATTAGVIRDLAEDRTPQLLADLRAEIPEGLVEITHLPSVGPRTVG
                     RLWKELGVTNVEELAALDPERISSLKGFGKKSAEKMVHAARTYNATERRMLLDEATSL
                     GEQILSFVRSHPATERAEVGGSLRRMKETVGDIDIVAASEDPDSLADDFAEAPFAEEV
                     LAHGPKKVFILRGGTEVDLRIVEPEAYGSLLHHFTGGQAHNIALRERAVKMGINISEY
                     GLAKAGTGDYKPVATEEDLYSRLGLA"
BASE COUNT         1643 a         2367 c         2536 g         1266 t
ORIGIN      
        1 cgcggcgacc gcgacgagaa ggcatgggcc ttcgagtcgg cggtggagaa ggcgctctgg
       61 tggatgctgc ggtcgacgct cgggcgggcg ttacgcctga tgctccgtct ctggcggggc
      121 agcaagatat tcaggattac gtcatacaca ggcgcgggca tattggtgac cgccgggata
      181 gccttcacga gcgtccggat ttatgagaac cggcacgcaa gcatacacgt cggtcccggc
      241 gcatacgaat tgagcctgcg aggtgaccgg ttctccatct acacggcgaa gggcattgag
      301 gtatggaacc tggataatag gcggcggata gggacggttc gtgatggaga tcagacacca
      361 gacttcatga gcccggacgg acggctgctc gccgggggag tgcagcacgg cgagtatccg
      421 gagcacactg tggaggacat ccggatacgg cgtacggaag atggttcgct tatacggatg
      481 atcgacaccg cgaaggacac cggctggccc gtgtggagcc cggacggcga gttgatcgcg
      541 gcacttaccc cggatgccat acagctcgtg aaggtacgcg acgggcggct ccttacgagc
      601 atcgagggct atccgccact ggcagaggat ccggtgccga tagtctggtc ccccgacgga
      661 cgtttcctcg ccatgaaagg cgaacggggc ggcatcgcgc tctgggacgt gaccagtggc
      721 cgctaccttc gcacgctcga aggtgacttc gagtcgggca tctccttcag cccggacggg
      781 cggctgttcg ccgagagcgg aaccccggtg aagctgagaa gcacgaccga tggtagcgtc
      841 gtccggaagc ttaggagtgg cgaaccagcc ggtccagtgt atgccagctc ggtgctcgcc
      901 ttctctcccg gcggcgagct cttggtgacg ggaggcgccg atggcaggat ccggctgtgg
      961 caagtgaatg acggcaagct cctcacagag tattcgaagc acgctcacaa cgagaccgat
     1021 gacacatcgt cggtgctcgc cgtcgccttc ttcccggatg ggcggcatat cgcctcgcac
     1081 gggtccgacg agacggtacg cgtctggccg gtcgaggatt acggactcga aggcgaacga
     1141 tagttggagg cgtcgcatgg gcgacgaaat aaagcttacc catcccaaca ttgtagagac
     1201 cgcacctacg agatcacacc gcactggcca cgtgcgggcg cagagtaaga aatgctttga
     1261 cgcgccttcg cgctgttgtt aggctcaata agtaccctcg ggaaaggaag agtatggcgc
     1321 tgaacgaggc actactcaag cggttgatcg agacgcccgg tgtctccggg cgggaggagc
     1381 ggcagcgcga gatagcacgc gaggagcttg gctctctgac ggatgaggtc cgcactgacg
     1441 ctcttgggag cgttatcggg acgaagaaag gtagcggcga cgggcgtgtg atggtcgccg
     1501 cgcacacaga cgagatcggc tacctcgtaa aacacataga cgacaagggc ttcgtgcgcc
     1561 tgcagaccct gggcggccat gatcccgcca acatggtctc ccagagggtc ctcgtaacga
     1621 ctgccgatgg cgagacgctc cgcggcgcgc tccagccgga gcgcaagccg ccgcacgtgg
     1681 cacgcggcga ggagcagaaa cagccgaagg ccggtgagtt ctacgtcgac ctcggcatgg
     1741 acgccgagac ggtcaaggag aaggtgcgaa tcggggactt cgtggtcatg gaccgcactc
     1801 tggagaaagt cggggagacc tacgtcggca aggccatgga cgaccgcgtc ggcgtcttcg
     1861 tgatgtacga agcgctgagg gctatgggag atcaccaggc gaccatcttc gccgccgcta
     1921 cctcgcagga ggaggtcggc ctacgtgggg ccgaggccag cggctcggct ctcgagccta
     1981 cggtagtcgt cgcgctcgac gtgacgctcg cgatggacgt gccaggcgcc ggtaaggaga
     2041 acaccataac tgagctaggc aaaggcgttg ccatcaagat catggacggc tactccatct
     2101 cccacccgaa gttggtggag catctccggg ccatcgccga gcgcgagaac atcccctacc
     2161 agatggagat tctccccgcc ggcggcaccg atgcgggcgg cgtacagcgc ctgcacggtg
     2221 gcatcccggc cgtcacgctc tctatcccct gccgctacgt ccacaccccg aacgagatgg
     2281 tgagcgccac ggacgtcgag gccgccataa cgctcctcgc ccgctacctc gaagagacct
     2341 cagacgccga ctacgcactc tagggcgtgg ataagaccct ggtcatcgag gagaacggcg
     2401 cagagttcct gatgacgctc ggtcgcgcgg ccggggccga ggaacgcgac gatgggcgag
     2461 tgcgttgggc catcgggaac tcccccatcg actaccacaa ctgcgtcgta catgccgacc
     2521 tgacccagaa cgaggcggac cgggagatcg aagcctccct gcaacgtatg cgggaacacg
     2581 aggtccccgg ctcctggcat gtaggacctt cgatgcgccc ccccgacctc ggctggcgcc
     2641 tcatcacgca cggtttcgaa tacggtggcg acgatatcgg gatggctgtg gacctctccg
     2701 cattgcccca tgaggtaccg gtgccggatg acttcgttat cgagcgggtg cgcgacgaag
     2761 cagggctcgc ggcgtgggtc gaggcgctgg gctcgggctt cggggagggg cctgtcgaag
     2821 ccgaatgggt cggggagatg taccgcaggt tagggttcga agggccgtgg cggcactatc
     2881 tcggtcgggt ggatgaagag cccgtggcca cagcaacgtc gttcttcggg gccggggtcg
     2941 cggggatcta cttcgtctgc accgtcgagc gtgccaggcg gcggggtatc ggggcgcgaa
     3001 ttacgctggc agcgttgagc gaagcaaggg atctgggcta cagcgtcggt gtgcttggtt
     3061 cctcggagat gggttactcc gtctaccgcg ggctgggctt cgaggaatac tgcaggatcg
     3121 ggctctacga gtggcgtcct gggtaggcgt cgccaaaacg ggcaccggat acggtagcat
     3181 gtgcgttcga cgctacgaca aataccaacg gaggggatct ggatggcaaa caagacctac
     3241 gaccgaccgc ctgagatggc gctagagcct ggctacgagt actacgccaa cctcgttacc
     3301 gagaagggcc ctgtgaaggt gaggctcttc gccgaggagg cacctgagac cgtcaacaac
     3361 ttcgtcttcc tggcgcgcga cgggtacttc gatgggacga ccttccaccg cgtcatagtg
     3421 gacttcatgg tccagggcgg ggacccgacg ggtacgggga caggcgggcc tgggtacagg
     3481 atccctgacg agttccatcc cgggctgcgt cacgacaggc ctggcatact ctcgatggcg
     3541 aacgcaggcc cgaataccgg cggttcgcag ttctttatca cgcacgtggc gacgccgtgg
     3601 ctagacgaca agcacgcggt cttcggggag gtcgtcgatg gtatggaagt cgttggcgcc
     3661 ataagggagc gggacccaca gcgcgaccgc gagccgggcg acaggataga gcggatcgag
     3721 atcgaagagc taccgcattc ctagagtttg gcatttagaa tctggaactg tccatcacgt
     3781 agccctttcc taatctaact tcacagttca acactgcata cgcacctgaa aggtgaaagg
     3841 tctacaacaa tccgatacgc tcgggtggcc agaagcgcag gaaagcctcg ccctctatgt
     3901 tcttctcggg cagtggtccg aagactcttg agtccgcgga gttgccgcgg ttatcaccca
     3961 tcacgaagac atgatccttc gggaccgtgg tcctggcgta gaagctcctg tcgggtggct
     4021 tcttgttcgt gtatggctcc ttctggggct caccgttcac gaagagcttg ccacccctga
     4081 cggcgatctt gtctcccggc agtccgacga cccgctttat caggtcctcg ttggagttat
     4141 cgacgctctc gaagactatg atgtctccgc gcctggggtc tgtgaagcgg tagatgaact
     4201 tgttcacgag cacccggtcg ccgatctcga gcgtcgggat catgctgcct gagggaatcc
     4261 agaaggcctc catgacgaat ggcctgacga agccgaagac cagcgcgaaa gatacgagca
     4321 gtatgaccag gtactcgagc gcccctccac ccttcttggt ggccggccga cgctcctcac
     4381 tggaggtctg gaactgctgg tcgtggtagt acggcttctc ggacatgaag tagtaggata
     4441 acgcaccggg acggatctga ctcaaaaagt ctccgttcct aaaatacgcc gagcgcctcg
     4501 ccgaggaggt ggagggccac gaccaccatg aacacgccga agccgattcg caccaaacgc
     4561 tctggaagcc agtccctgat gcgtaccccg aggggggcac ctatcacgga accgataatg
     4621 aggggcggca ggctgagcag gtcctgcact tccctgaagc cggtcgccac gtagccggca
     4681 gcccctacga tacccgtgag catcacgacg gcgaggctag tagagacggc ctgcttggtc
     4741 gtgagcccca agccaagcac catcagcggg accataacag ccccgccgcc gaccccgacg
     4801 agcccagaga gcgcccctac aaagatacca gccaggatga ccaggggtag gggtaagttc
     4861 ttgcgatcac tgtcgtattc gcccctgcca cgggcggtcg ggtaggcgag ggatatgagt
     4921 agcgtggcga acgccacctt cacgacgacc tcaggggaga cgcggcttat aaggaccccg
     4981 atcaaagacg aaggggcgac cgccagggag aggaggccag cgacccgcca gttcacgggg
     5041 tccgcactcc tggcgttgcg aatcgtgcct gatagggagc tgaagatgat gatcaccaag
     5101 ctcgcggcga cggcatcttg aatgcgccat ccgccgacgc acaacagacc agggacgaag
     5161 acgatcccgc caccgacccc gacgagccca gagagcatcc ccccaacaat cccgatgacc
     5221 ccaaggaaca ggaactctag ataatccacc gcgctattct aaatcagcat gtcagcacgc
     5281 gctctgtcag gcgcttgtca gcacatcagc cagtcagctt tcgtgtgcgg aagccctatc
     5341 tgagaagcga aggcgaagtc tgagcgtgct gaaacgctct aagacgcgga gcgcctccag
     5401 cgccagaaca ggtagagggc tccggccacc agcgccatcg cgacggcgta gtcgaggtag
     5461 tgcagatagt cgcctatcgt cgtccagttc tcgccgagca cgtacccgaa gtacgtcagc
     5521 gcgaagaccc atggtatgca ccccaggaag gtgtagaggg tgaacctggc gaaattcatt
     5581 ctggcggtgc cggcggggaa tgagataaac gtgcggacca ccggcaggca gcgggagacg
     5641 aagacggtga actcaccgta cttatcgaac cacttctcgg ctatatgcag gtgatgcgcc
     5701 ctgacgccca cgtatctgcc gtagtggaac cagagctcgc gcccgcccca gagcccgagg
     5761 aagtaagcga tccaggagcc gaccacgttc cccaggactc cggcggtcac cgccgccagg
     5821 aaactcatct ctccctccga gaccagataa cccgcgaagg gtgagatcgc ctccgagggg
     5881 atgaggatac ccatgctctc cagcagcatg agcacgaaga ccgcagggac gccgtagctt
     5941 ccgatggtct cggtgacgaa ctctatgagc ggatgtagga tcgcttcgag cacgatgaga
     6001 agctaccact tagagacacg gatgtacaat gcctatccgc catctttttc ccgaggccgc
     6061 cagaacccta tagattaagt atgctatcca tcagggtctg atattacccg tcgctgcggc
     6121 tcgactcggc cgcccccaag ctccggacgg agagccacag cgcgtacacg gcgccgacgc
     6181 agaccagcca gaggagggca gcgaggtagc cagccactat gtctgtcggg tagtgcaccc
     6241 cgaggtaaag cctggaaaag ccgatcagga acacaacgag tattcctgag accaccacgg
     6301 cccatcgcgc tgtcccccgc agccggtaag ccaggacgag cgtgagcatc ccgtacaacc
     6361 cgacggcgac ggtggcgtgg ccgctcggaa acgaataaaa ggaggcgtgg tagccggagt
     6421 cgaagagctc gggacgtgcc cgctgaaata cgcttttcag gacggtggtg agcacggcac
     6481 tcccagcggt cgagatcagg aggagtatgg ccgagagcct ccagcctctt tggtagaaga
     6541 gaggtacgac cacgaccaag agtagcacca cgaaccagta gtagccgagc gccgtgacga
     6601 tacgcatcgg cccgtcgagc cagctgggga aggtcgtgtg gatccagagc agcacggccc
     6661 tgtctaaggc gcggctctcg ccctcgacaa ccacgtcggc cagctcggta aaggcccaga
     6721 taacgaaggc cgaaaacgtg agccccgccg caagccaggc tgcggcgacc accgtcaggc
     6781 ggtccgcgac ccaccggact atctctctcc ttccctcgag catggagaga ttctaacccg
     6841 tttcggtacc ctggcggcgc gtataatctc tgtggctgac ggcgcgtgcg gaggaggagc
     6901 gagcaccttg caaaacaacg acatcgtccg ggcgctagag acgatcgcga cgctcatgga
     6961 gatcaaagac gaagcgtact accgcgtgct cggctaccag cgggccgccg agtcggtggc
     7021 agccctaggg cggccggcga ccgaggtcga tgacctcaag acgctccccc acgtcggcgc
     7081 gacgacggcc ggcgtgataa gggatctggc cgaagacagg acgccccagc tactcgccga
     7141 cctgagggcc gagatacccg aaggcctggt ggagataacg cacctgccta gcgtcgggcc
     7201 gcgcacggtc ggacgcctgt ggaaggagct gggcgtcacc aacgtcgagg agctcgcggc
     7261 cctcgatccg gagcggatct cctccctcaa aggtttcggg aagaagagcg cagagaaaat
     7321 ggtgcatgcc gccaggacgt acaacgcgac ggaacgacgg atgctcctgg acgaagcgac
     7381 ctctttgggg gagcaaatcc tctcgttcgt ccgctcgcac ccggcgaccg agcgggcaga
     7441 ggtcgggggc tccctgcgcc gcatgaagga gaccgtaggc gatatcgaca tcgtcgccgc
     7501 aagcgaggac cccgactcgc tcgccgacga cttcgcagag gcgccgttcg ctgaggaagt
     7561 cctggcccac ggcccgaaga aggtctttat cttgcgcggc ggaaccgagg ttgacctgag
     7621 aatagtcgag cccgaggcct acggatcgct cttgcaccac ttcaccggcg gccaggccca
     7681 caacatcgcg ctgcgcgagc gggccgtcaa gatgggtatc aacatctccg agtacggcct
     7741 ggccaaggcc gggaccggcg actacaaacc cgtggccacg gaggaggatc tctactcgcg
     7801 gctcggcctc gc
//
LOCUS       DASTKM010000017         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_707167, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000017 DASTKM010000000
VERSION     DASTKM010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707167"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VFQ10_00605"
     CDS             complement(<1..1158)
                     /locus_tag="VFQ10_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HET9963866.1"
                     /translation="MDLAVRKMTLGRFGVEDVGAVHENLPPARLVEASVRRREGMISG
                     SGALVVRTGKRTGRSPKDRFIVENGVTRETVDWGTGNKAFPSNAFGALLDKVAAYVEN
                     LEEIYVVDAYAGADPRYRLNVQVVTEHAWQALFARQLFRRPSKAEIDSFEPDWTVISV
                     PGFLTDPEEDATESETFVGIDFERKVVLVCGTAYAGEIKKSIFTVLNFVLPTEHGVFP
                     MHCSANVGKEGDVALFFGLSGTGKTTLSAESERDLIGDDEHGWSDSGVFNFEGGCYAK
                     TIDLSAEKEPQIWDAIRFGAVLENVVMDRRTREVDYSDRMLTENTRVAYPLEYIAGAV
                     TEGGGVHPSAVLFLTADAFGVLPPISALTPSQAAYYFLSGYTAKLAGTEAEM"
     gene            1363..>3017
                     /locus_tag="VFQ10_00610"
     CDS             1363..>3017
                     /locus_tag="VFQ10_00610"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET9963867.1"
                     /translation="MAQQINQETAGAEQWRALAQQLRIDSIRSSSGAGSGHPTSSMSA
                     ADLIAVLLSKYLSYDFDDPENPNNDHLIFSKGHASPLLYSAYKAAGAISDEELMTFRR
                     FRSRLQGHPTPQIPWVDVATGSLGQGLPIGVGVALAGKYLDKLPYRVWVLCGDSEMAE
                     GSMWEAFQHASYYKLDNLIAILDMNRLGQTRETMDGWNGDHYVARAEAFGWNTIQIDG
                     HDPEQIDQAYAQALEQTEAPTLIVAKTKKGRGVSFLEDVDGMHGKPVSEDQEQAALEE
                     LGSTDDLLVEVRKPDPGSGETFTAGLTGRLELPTWEVGDEEATRGAYGAALKALGGAR
                     EDVVALDGEVSNSTKSEEFAEAHPERYFEMFIAEQQMVAAAVGMSVRHRVPFASSFAA
                     FFTRAYDFIRMAAISGANIRLSGSHAGVSIGEDGPSQMALEDLAMMRAVHGSTVLQPC
                     DANQTARLVAEMADTEGIAFLRTLRPKTPVIYDPSEDFPIGGSKIVRSSEEDVVSIVA
                     TGITVHEAIKAADELQDSGVAVRIIDAYSIKPIDGESLRAAARA"
BASE COUNT          571 a          983 c          935 g          528 t
ORIGIN      
        1 catctcggcc tcggtaccgg cgagtttggc ggtgtagccg gagaggaagt agtaggctgc
       61 ctgcgaaggc gtgagggcgc tgatgggggg caacaccccg aaggcgtctg cggtcagaaa
      121 gaggacggcc gaaggatgaa cgcccccgcc ttcggtgact gcgcccgcta tgtactccaa
      181 cgggtaggcg acacgggtgt tctccgtcag catgcggtcg gagtagtcga cctctcgcgt
      241 gcgcctatcc atcaccacgt tctcgagcac ggcgccgaag cggatggcgt cccatatctg
      301 gggttctttc tcggcactta gatctatcgt cttcgcgtag caaccgccct cgaagttgaa
      361 gaccccagag tcggaccagc cgtgctcgtc gtcgcctatc aggtcccgct cggactcggc
      421 cgagagggtc gtcttgcccg tccccgagag cccgaagaag agcgcaacgt ctccttcctt
      481 tcccacgttg gccgagcagt gcatcgggaa gacgccgtgt tcggtcggca acacgaagtt
      541 gagcacggtg aagatgctct tctttatctc ccccgcgtag gccgtaccgc aaacgagtac
      601 taccttgcgc tcgaagtcta tcccgacaaa ggtctctgac tctgtcgcat cctcctcagg
      661 atcagtaagg aatccaggta ccgagattac ggtccagtca ggctcgaatg agtcgatctc
      721 cgccttcgag gggcgtctga atagctgcct ggcgaacagg gcctgccagg cgtgctcggt
      781 caccacttgg acgttcaggc ggtagcgggg gtctgcgccg gcgtaggcgt cgactacgta
      841 gatctcctcc aggttctcga cgtaggcagc caccttgtcg agaagcgccc cgaaggcgtt
      901 gctagggaaa gccttgttgc ccgtacccca gtccacggtc tccctcgtaa ccccgttctc
      961 gacgataaag cggtccttcg gggagcgtcc ggtccgtttg cccgtcctga cgaccagggc
     1021 tccagaacca gagatcatgc cctcccgcct cctgacagag gcctcgacga ggcgcgcagg
     1081 aggcaggttt tcgtgtacgg ctccgacgtc ctccaccccg aagcgtccca gcgtcatctt
     1141 gcgaaccgcc agatccatag ttcacccttt cacccggtcg tacctgacgc gtagaacgca
     1201 cgctcagggt ctttttcctc tcgccctcag gattctagca aagcctgtaa gccctcgtct
     1261 gtagggtcga tacctcacgc agcagggcag tttccgaatc gccagcgcgg ggtaggagaa
     1321 gtactatctg tgctttttgc gattctgccg aggaggttag tggtggcaca acagatcaac
     1381 caggagaccg cgggtgccga gcagtggcga gcgctcgccc agcagttgag gatcgacagc
     1441 atccgttcga gctctggcgc cggctcgggg catcccacct cgtccatgtc ggccgccgac
     1501 ctcatcgccg tgctgctgag caagtacctc agctacgact tcgatgaccc ggagaacccc
     1561 aacaacgacc acctgatctt ctccaagggc cacgcctccc cgctgctcta ctcggcatac
     1621 aaggcggcgg gcgcgatctc agacgaagag ctcatgactt tccgcaggtt ccgcagccgc
     1681 ctgcaagggc acccgacacc ccagatccca tgggttgacg tggcgacggg ctccctgggc
     1741 cagggactcc ctataggcgt cggggtggcg ctggccggca agtacctgga taaactcccc
     1801 taccgggtct gggtgctttg cggcgactcg gagatggcgg agggctcgat gtgggaggct
     1861 ttccagcacg cctcttacta caagctcgac aacctcatcg ccatcctgga catgaaccgc
     1921 ctcggccaga cccgcgagac gatggatggc tggaacggcg accactacgt cgcccgcgcc
     1981 gaggccttcg gctggaatac gatccagatc gacggccacg accctgaaca gatcgaccag
     2041 gcctacgcgc aggcgctgga acagaccgag gcgccgaccc tgatcgtcgc gaagaccaag
     2101 aagggacgcg gggtctcgtt cctggaagac gtggacggca tgcacggcaa gccggtttcg
     2161 gaagaccagg agcaggcggc gctcgaggaa ctcggaagca ccgacgacct gctggtcgag
     2221 gtacgcaagc ccgaccctgg ctcgggcgag accttcaccg caggccttac cggccggctc
     2281 gagctgccga cctgggaggt cggcgacgag gaggcgaccc gaggggccta cggcgcggcc
     2341 ctcaaggcgc tgggtggcgc cagggaggac gtcgtcgccc tcgatggcga ggtcagcaac
     2401 tcaaccaagt ccgaggagtt cgccgaggct catcccgagc gctacttcga gatgttcatt
     2461 gccgagcagc agatggtcgc ggcggccgtg gggatgagcg tacgccacag ggtccctttc
     2521 gcctccagct tcgccgcttt cttcacccgc gcgtacgact ttatcaggat ggccgcgatc
     2581 tcgggcgcca atatccgtct ctccggctcg catgctggcg tctccatcgg tgaagacgga
     2641 ccatcacaga tggccctcga ggacctggcg atgatgaggg ctgtgcacgg ctcgaccgtg
     2701 ctccagccct gcgacgccaa ccagaccgcc aggctcgtcg ccgagatggc cgacacggag
     2761 ggcatcgcct tcctgaggac gctgcgcccg aagacgcccg tgatctacga tccctcggaa
     2821 gacttcccta tcggcggaag caagatagtg cgctcctcag aggaggacgt ggtgagcata
     2881 gtcgcgacag gcatcacggt ccacgaagcc atcaaagccg cagacgagct gcaagatagc
     2941 ggcgtggccg tgcgcatcat agacgcctac tccataaagc caatagacgg cgagtcgcta
     3001 cgcgccgccg cacgggc
//
LOCUS       DASTKM010000018         8776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_2835, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000018 DASTKM010000000
VERSION     DASTKM010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2835"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1224)
                     /gene="ftsA"
                     /locus_tag="VFQ10_00615"
     CDS             complement(22..1224)
                     /gene="ftsA"
                     /locus_tag="VFQ10_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET9963868.1"
                     /translation="MAQTLVHGIDVGTTKVVAIAGRVDPRRGWVEVLSMGEAPSYGLK
                     RGVVVDRKAAADSVAEAMEACGVRGGKVTVGIAGSHISSYNKEVTLLNRSRNLTVTDR
                     FVRRLEQEARQLDLHEDERVIHVVPRGYVLDGSEGVKNPAGLAARKVTMRAHVVSGAV
                     SSIQNLLRAVEDCGVKVSRIVLEPLASAEACLLDADRESGVVLLDVGGGTTDIATFYR
                     GALTHTDVIPIGGESFTSDVAYGLKIPQHKAEELKLRYGTVLSSTVDPVAAVKLDDRH
                     YNASFVSQILEYRAREVLDYVRDSLDQTMSRELAPGGVALTGGSSLLDGMTGLAEEVL
                     GMRAKTVSPRRVRGEVEPVQQPQYATAVGLLYFSARYDNPLSKGKGAGAYSFGSIVEA
                     VKGWFRGM"
     gene            complement(1231..1830)
                     /locus_tag="VFQ10_00620"
     CDS             complement(1231..1830)
                     /locus_tag="VFQ10_00620"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET9963869.1"
                     /translation="MTYLAFPVTGVRVEDARMYPESEVIDAIPDHASLLTLNEEMLKR
                     DLESHPWVKGTEVNEDWNSGIVTVQVEERRPVLDAEVDGRRFVLSADGEELPGPGGAG
                     LERVELDRDQVGEILVFARTLRRSGLTLESVDGAGAGGIEATVEGREVIFSGNVGERR
                     VMALKHIMPEHPDARVFDLRTPERVVVGAPHETRSEPKG"
     gene            complement(1899..3263)
                     /gene="murC"
                     /locus_tag="VFQ10_00625"
     CDS             complement(1899..3263)
                     /gene="murC"
                     /locus_tag="VFQ10_00625"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET9963870.1"
                     /translation="MNIHMIGIGGAGMSGIAEVLSRRGHSVTGSDLKESPYTRRLREA
                     GITVYIGHAPDQVGDADRVVISTAIPKTNPELLQARRLSIPVIPRAAALAWILEGSRS
                     VAVAGTHGKTTTTSMTAHVLEALGENPTALVGGELNDIGSNVAFGRSDLVVAEADESD
                     RSFLYLRPQAAVVTNIEFDHPDFYSSLDDVVETFEQFVGSLPKDGHLIVCSDDPSCPK
                     VAAEAPCPVTTYGIGSGDLSADIVSPSSYVLFEDGERQGEVELGVYGRHNVLNSLAAA
                     GVARWLGHQAHEAASTLQNFGGVRRRFQLKGEQAEVRVVDDYAHHPTELSATLAAARS
                     TMPGYGRVIAVFQPHRYSRTLALYREFGASFSSADAVLVTEVYGAGEMPQPGVNGKLI
                     VDAICETRGHPEVYYIPQQDAIPAVLQNVSRRGDLVLTLGAGDISKAGEDLLGILEEQ
                     GSKP"
     gene            complement(3269..4324)
                     /gene="murG"
                     /locus_tag="VFQ10_00630"
     CDS             complement(3269..4324)
                     /gene="murG"
                     /locus_tag="VFQ10_00630"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET9963871.1"
                     /translation="MPRILIAGGGTGGHAIPALCVAADLKERGAIVEFVGSSSGIEMS
                     LVPQQGYTLHALPLTGLAGSPLDQARAGLLSLRAVIRCTRIIQRLRPLSVLGVGGYAS
                     APAVIAARTLGVPTFLHEQNSVPGRVNRLASRFTTQTLTTFPDAARRLGNAVWVGMPT
                     RTKVFEASQDEALRYLGLQPPVVLIFGGSGGALRLNLAAAEAFGHATPYTVVQISGRR
                     DFSRLSTDNPRHTILEFTGEIWRYLAAADVIVSRAGAGSLFDIAAAGRAAILVPFPYA
                     TGDHQLENARYFTERGAAELMLDSEVGARALRDRVEDLLDDDARREELARRMRTLATP
                     GAAGEVARRLLGAGEKE"
     gene            complement(4254..5402)
                     /locus_tag="VFQ10_00635"
     CDS             complement(4254..5402)
                     /locus_tag="VFQ10_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HET9963872.1"
                     /translation="MSSLRSNLFLVALGLSLLGLVMVYSATYSEVGTDFLFERIGHMV
                     LGVAAFFVASRVRYTAWRKYAPALYLIVLVGLVLVLIPGIGTQINGARRWLDLGLMSI
                     QPAEFAKLAAILVLSCAVARLPHGSGLPVKPLVGVGVLFILVFVEPDFGTSVVLLSGV
                     AGTLWASELRTRDLVLCGLIGLVVVAGMMVLEPYRRERFLTFLDPWAAVDGSGYQVVQ
                     SMVAIKAGGFFGSGAGSGGQGPYVPELGTDMIFALIGEELGLIGMIVVIAAFGFIALA
                     GLRIAMNAPSVMARCVAAGLTTMLIAQATFNIGAAMVVLPLAGMTLPFVSYGGSSLLV
                     CFAAVGILYRISEDSERASEIKPRKNAADPDSRRRHGGARYPRAVRGG"
     gene            complement(5399..6721)
                     /gene="murD"
                     /locus_tag="VFQ10_00640"
     CDS             complement(5399..6721)
                     /gene="murD"
                     /locus_tag="VFQ10_00640"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET9963873.1"
                     /translation="MRTLVYGLGESGVAATRALTERGKAVLAADGGDDERLRDTLAAL
                     HVSGVLGATPHVLEGIDRIIASPGVSPRNAVLRAAGARGIPIISEVGLGLELLGEDAR
                     VVAVTGTNGKTTVVDMLHRMLEASGVPHAVAGNSWRPLTGCLEEARAAGLLVLEVSSF
                     QLHYLENPGFEVAALLNVRPDHLNWHESFEEYTNDKLRIFEGQQEEDLALVSAADPIS
                     SAAIHTFRAEALVVGEGDTALRDGRLLLRGSHLAEVTELRFAGMHNHENALFAAAAAQ
                     RLGAELGEIREALLEYESRPHRMRVAARMSGVTYVDDSKATNPAAVAAALESFDVPVV
                     LILGGSEKYTDFSEVLPALGVCRAVVCQGEAGPRIAAYLEDEGFQSVVHRTPDLEKAV
                     ARAIELARPGDVVLLSPGCASFDQFPGYAQRGDAFIEELATHHGTVGR"
     gene            complement(6726..7721)
                     /gene="mraY"
                     /locus_tag="VFQ10_00645"
     CDS             complement(6726..7721)
                     /gene="mraY"
                     /locus_tag="VFQ10_00645"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET9963874.1"
                     /translation="MFSVVLAAAVAFLVTVLLGSKFIEFLQSRKFGQFVREEGPQTHL
                     IKSGTPTMGGIVMLTGLLGALVVVARPNVATLTTLLLVAVVAGIGLYDDWQKISRKRP
                     EGLSARYKFLLLTLAVVLADVLAVRYVGVTQTVLVPYFDRSLVLGPGVMGIALFSVFL
                     LLVIAGTTNAVNLTDGLDGLAAGAAAIALLAYTAIAFLERQYDVAVICGAMVGAIVGF
                     LWYNSHPADIFMGDTGSLAIGGVLAAAAVLTKTELLLPIVGGLFVVEALSVIVQVFVF
                     KLTGRRVFKMAPIHHHFEFLGWDENKVVVRFWIAQAAFSSVGFFLYYIFLYNTAG"
     gene            complement(7714..>8776)
                     /gene="murF"
                     /locus_tag="VFQ10_00650"
     CDS             complement(7714..>8776)
                     /gene="murF"
                     /locus_tag="VFQ10_00650"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET9963875.1"
                     /translation="VGITGTVGKTTTKDALATILRHAGKRVSATAGNLNNEIGLPLTI
                     LASDPRTEVLVLEMGATHAGDIKSLCTIAPPDIGVLTAISPVHLDSFGTLEGLAAAKG
                     ELALSLPESGTLVAPAGAPETATGSDRKLGRRITFARAGEADLVASDIEGRDEGLRFV
                     ASIAGESAEVRSPVFGTHLVEPLLAALGGTLALGLGLEQAARGLSRLRRTGLRGDVYN
                     LREDITVYDDSYNASPAAVEAVLRYGADQARRQRRRLVVVLGGMFELGACARAYHREA
                     GELAARVGVDLLVCVGDEARWYAETFPGRTLFYPDAESAADGLRGELRARDYLIVKGS
                     RGVGLDTLTRKLRESLALV"
BASE COUNT         1647 a         2854 c         2712 g         1563 t
ORIGIN      
        1 cattcttcga ggcccccggg tctacattcc ccggaaccag cccttaaccg cctccactat
       61 gctaccaaaa ctatacgccc ctgcaccttt accctttgat agagggttat cgtatctggc
      121 cgagaagtat aacagaccca ctgccgttgc atactgaggt tgctgtacag gttcaacctc
      181 acctctgact cggcgagggc ttacggtctt tgcgcgcata ccgaggactt cctctgcgag
      241 ccctgtcatt ccgtcgagca aggagctgcc tccggtaagg gcgacgccgc ctggggccag
      301 ctccctcgac atggtctgat caagcgagtc gcgcacgtag tcgagcactt cgcgggcgcg
      361 gtactcgagg atctggctta caaagctggc gttgtagtgg cggtcgtcga gcttgacggc
      421 ggctacggga tcgacggtgc tggagaggac tgtaccgtag cgaagcttga gttcctcggc
      481 cttgtgttgc ggaatcttga ggccgtaggc gacgtcggat gtgaagctct caccgccgat
      541 gggaatgaca tcagtatgag tgagagcccc cctatagaag gtcgcgatat cagtcgtgcc
      601 gccgcccaca tcgagaagta cgacacccga ttcgcggtca gcgtcgagca ggcaggcttc
      661 ggccgaagcc agtggctcga gcacgatcct cgaaaccttg acgccgcagt cctcgacggc
      721 gcgcaggagg ttctggatgc tggagaccgc cccactcaca acgtgcgcgc gcatggtcac
      781 cttgcgggcg gcgaggcctg cgggattctt gacgccctca gacccgtcga ggacgtagcc
      841 cctgggcacg acgtgtatca cccgctcgtc ttcgtgcagg tcgagctgcc tggcctcctg
      901 ttcgagcctg cggacgaacc tgtctgtaac cgtcaggttc ctgctgcggt tcaggagcgt
      961 aacctctttg ttgtaagaag agatgtggct gcccgcgatg ccgacggtca ccttaccgcc
     1021 gcgaacgcca caggcttcca tggcttcagc gaccgaatcg gcggcggctt tgcggtctac
     1081 cacgaccccc cgcttgaggc cgtagctcgg cgcctccccc atggatagga cctcgaccca
     1141 gccccttcgc gggtccaccc ggccggcgat cgccactacc ttggtggtgc cgacgtctat
     1201 tccgtggact agcgtctgcg ccacggctgc ctagcccttt ggctcgctgc gcgtttcgtg
     1261 cggggcaccg acgaccactc tctccggcgt acgcagatcg aagaccctgg cgtcgggatg
     1321 ctctggcatg atgtgcttca aggccatgac ccgcctttca ccaacattcc cagagaagat
     1381 gacctcgcgg ccttccaccg tggcctcgat gcccccggca ccagccccat cgacagactc
     1441 cagtgtcaac ccgctcctgc gcaacgtcct cgcgaacacc aagatctccc ctacctggtc
     1501 gcgatccaac tccaccctct cgagacccgc tccccctggc cccggaagct cctcgccgtc
     1561 agccgaaaga acgaacctcc gcccgtcaac ctcggcatca agcacaggtc ttcgctcctc
     1621 aacctgaacc gtaactatac cggaattcca atcctcgttc acctctgtac ccttaaccca
     1681 aggatgagac tccaggtcgc gtttcaacat ctcttcgtta agggtgagca ggctggcgtg
     1741 gtcagggata gcgtcgatga cttcggattc ggggtacata cgagcgtctt ccaccctgac
     1801 gccggtaacg ggaaaagcaa ggtaggtcat caggacgagg atgagggtgg tagcggcggc
     1861 tgtggccgtc gagatcagga tggcgcgcag agttcttgtc agggtttgga tccttgctcc
     1921 tccagtatgc cgagcaggtc ttcaccggcc ttggagatgt cacctgcgcc gagggtgagc
     1981 acgaggtctc cccttctgga gacgttttgc agcaccgctg gtatggcgtc ttgctggggt
     2041 atgtagtaga cctcggggtg gcctcgcgtc tcgcaaatag cgtcgactat cagcttgccg
     2101 ttgacgcctg gttggggcat ctcaccggcg ccgtagacct cggtgacgag cacggcgtcg
     2161 gccgaggaga acgaggcgcc gaactccctg tagagggcga gggtacgtga gtaacggtgc
     2221 ggctggaata cggcgatcac ccttccatac cctggcatgg tggaacgagc ggcggcgagt
     2281 gtggccgaga gctcggtcgg atggtgggcg tagtcgtcga cgacgcgtac ctctgcctgc
     2341 tctcctttca gctggaagcg gcggcgcacg cccccgaagt tctggagggt gctggccgcc
     2401 tcgtgagcct ggtggccgag ccagcgggca acaccagccg ccgccaggga gttgagcacg
     2461 ttgtggcgcc cgtagacgcc cagttcgacc tcgccttgtc tctcaccgtc ctcgaacagc
     2521 acgtaactgc ttgggctcac tatgtctgca ctgaggtctc cggagccgat gccgtaagta
     2581 gttacggggc atggagcctc ggcggccacc ttggggcagg aagggtcatc cgagcagacg
     2641 attaggtggc cgtccttggg cagagagccg acgaattgct cgaaggtctc cacgacgtcg
     2701 tcgagagacg agtagaagtc agggtggtcg aactcgatgt tcgtgaccac ggcggcctgt
     2761 ggcctgaggt agaggaagga acggtcgctc tcatctgcct cggccacgac gaggtcggac
     2821 cttccgaatg cgacgttgct gccaatgtcg ttcagttcgc cgccgaccag ggccgtcggg
     2881 ttctcgccca gagcctcgag gacgtgcgcc gtcatgctcg tcgttgtcgt cttgccgtgg
     2941 gttcctgcca cggcgacgct gcggctgcct tcgaggatcc aggccaaggc tgcggcgcgc
     3001 gggataacgg ggatggacaa ccgcctggct tgcagaagct ccgggttcgt cttgggtatg
     3061 gccgtcgaga tcaccacacg gtctgcgtcc cccacttgat ccggggcgtg ccctatgtaa
     3121 acggtgatgc cagcctcgcg cagcctgcgg gtgtaaggag actccttgag atccgagccc
     3181 gtgacgctat ggcctcttct agacaggacc tcggcgatac cgctcattcc cgccccgccg
     3241 atgccgatca tgtgtatgtt catgggcctc actccttctc tccggcaccc agcaatcgcc
     3301 gggctacttc tcctgccgcg ccaggcgtgg cgagcgtgcg catgcgcctg gcaagctcct
     3361 cccgccgcgc atcatcgtcc aacaagtctt cgacgcggtc gcgaagtgca cgtgcgccaa
     3421 cctcggagtc gagcatcagc tccgcggccc ctctttcggt aaagtacctg gcgttctcga
     3481 gctggtggtc gcccgtcgcg taggggaagg ggacgaggat cgccgccctt ccggcggccg
     3541 caatgtcgaa cagagagccc gcgccggccc tgctaactat cacatccgcg gcggccagat
     3601 accgccagat ttcgcctgtg aactcgagga tcgtgtgcct cgggttgtcc gtggagagcc
     3661 gtgagaaatc cctgcggccc gagatctgga cgaccgtata cggcgtcgcg tgcccgaacg
     3721 cctccgccgc tgcgaggttg agtctgagtg cccccccgct cccgccgaag atgagaacga
     3781 ccggcggctg caggccgaga taccgcagtg cctcatcctg cgaagcctcg aagaccttcg
     3841 tccttgtcgg cataccgacc cacacggcat tcccaagccg ccgcgcggca tcaggaaacg
     3901 tggttagtgt ctgtgtcgtg aagcggcttg ccaggcggtt gacccgcccg gggacagagt
     3961 tttgctcatg caggaacgtg ggcacgccga gcgtcctggc cgcaatcacc gcaggcgcgc
     4021 tcgcgtagcc tccgacgccg agaacggata gcggcctcag ccgttgtatt atgcgtgtac
     4081 agcggatgac ggctctcagg gagaggaggc ctgcgcgggc ctggtccagc gggcttccgg
     4141 cgaggcccgt caggggcagg gcgtgcaggg tgtagccttg ctgtgggacc agggacattt
     4201 ctatgccgga gctagatccc acgaactcga ctatcgctcc cctctctttc aaatcagccg
     4261 ccacgcacag cgcggggata gcgtgccccc ccgtgccgcc tcctgctatc aggatccgcg
     4321 gcattcttgc gtggtttgat ctcgctggct cgttcactgt cctccgatat cctgtagagg
     4381 atacccacgg cggcgaagca taccagcaga ctcgatccac cgtaactaac gaaagggagc
     4441 gtcatacctg cgagcgggag aacgaccatg gctgccccga tgttgaaggt ggcctgggcg
     4501 atgagcatcg tcgtcaggcc tgcggccacg cagcgcgcca tcaccgaagg agcgttcatg
     4561 gctatcctca gcccggccag cgcgataaaa ccgaaggcgg cgatcacgac gatcatcccg
     4621 atcagaccga gctcctcacc gatcagggcg aagatcatat cggtgcccaa ctctgggacg
     4681 tatggacctt gtccgcccga ccctgccccg gaaccgaaga agcccccggc ctttatggcc
     4741 accatggact ggacaacctg gtagccggag ccgtccaccg cggcccaggg atcgaggaac
     4801 gtcaggaacc gctcgcggcg atagggctcg agcaccatca tccccgctac cactaccaac
     4861 ccaatcagcc cacacagtac caggtccctc gtcctcagtt cggaggccca cagcgtacct
     4921 gccacgccag agagcaggac aacagaggtg ccgaaatctg gttcgacaaa gacaaggatg
     4981 aagagcaccc cgacccccac gaggggcttg acgggtaacc ccgaaccatg tggcaggcgc
     5041 gcgaccgcgc agctcaagac caggatagcg gcgagcttcg cgaactccgc aggctgaatg
     5101 ctcatgaggc caaggtccag ccaccgcctg gcgccgttta tctgggttcc tataccaggt
     5161 atgagtacga ggacgaggcc taccagtacg atcaggtaca aggcaggggc atacttgcgc
     5221 cacgctgtat acctgactcg gctggccaca aagaaggcgg cgacgccgag taccatgtgc
     5281 ccgatgcgtt cgaacagaaa atcggttcca acctcactgt acgtagccga atagaccata
     5341 acgagcccaa gcaaggaaag gccgagcgcc acgaggaaca ggttggacct caggctactc
     5401 atcgccccac agtcccgtga tgtgtggcga gctcctcgat gaacgcgtct cccctctggg
     5461 cgtatccggg gaactggtcg aagcttgcgc acccgggcga gagcaggacg acgtcacctg
     5521 gcctggcaag ctctatagct cttgcaacgg ccttctccag gtcgggagtc ctgtggacta
     5581 cgctttggaa gccctcatct tcgaggtagg cggcgatgcg tggacctgcc tcaccctggc
     5641 acacgaccgc ccggcacact cccagggcag gcagcacctc cgagaagtcg gtgtacttct
     5701 ccgagccacc gaggatcagc actacaggca cgtcgaagct ctccaaggcc gctgccaccg
     5761 cagccgggtt ggtagccttg gaatcatcca cgtaagtcac tcccgacatc cgtgccgcga
     5821 cccgcatgcg atgcggcctg gattcgtatt cgagcagagc ctctctgatc tcccccagct
     5881 ccgcacccag cctctgtgcg gcggccgccg cgaagagcgc gttttcgtgg ttgtgcatcc
     5941 ctgcgaagcg cagctcggtc acctctgcca gatgagagcc gcggagtagt agacgcccgt
     6001 cgcgaagggc cgtgtcgcct tcgcccacga cgagggcttc cgccctgaag gtgtggatag
     6061 cggcactgct tatgggatct gctgcgctaa cgagagccag gtcctcttcc tgctgtccct
     6121 cgaagatacg gagcttgtcg ttggtgtact cctcgaacga ttcgtgccag ttcaggtgat
     6181 ccggcctcac attgaggagc gccgcgactt cgaagccggg gttctcgaga tagtggagct
     6241 ggaaggaaga gacttcgagg acgagcagcc ccgcagcacg cgcctcctcc aggcaccccg
     6301 taaggggtct ccaggagttg ccggcgacgg cgtggggcac gccggaagcc tcgagcatgc
     6361 ggtgaagcat gtcgacgacg gtggtctttc cgttggtgcc ggtgacggcc accacgcgcg
     6421 cgtcctcacc gagaagttca agaccgagcc caacctcgga gatgatcggg atgccccgtg
     6481 ctccggctgc ccgcaaaact gcgttgcggg gactgacgcc agggctcgct atgatcctat
     6541 caatcccttc gagcacgtgg ggggtggccc cgagtacgcc cgacacgtgg agagcggcca
     6601 gagtgtctcg cagccgttcg tcgtcgccac catcagccgc gaggactgct ttgccccttt
     6661 ccgttagagc ccgcgtggcc gcgacgcccg actctccgag cccgtatacc agggtcctca
     6721 cgagctcacc ctgccgtgtt gtagaggaag atatagtaga ggaagaaccc cacggaggag
     6781 aaagcggcct gggcgatcca gaatcggacc accaccttgt tctcgtccca gcccagaaac
     6841 tcgaagtggt ggtgtatcgg ggccatcttg aacacccttc gtcccgttag cttgaagacg
     6901 aagacctgaa cgatgaccga gagagcctcg acaacgaaca gcccgccaac gatgggaagg
     6961 aggagctccg tcttggtgag caccgccgcc gccgcgagaa cgccccctat cgcgagcgag
     7021 cccgtatccc ccataaagat gtcggccggg tgggagttgt accagaggaa cccgacgatc
     7081 gccccaacca ttgcgccgca gatcacggcg acgtcgtact ggcgctcgag gaaagcgata
     7141 gccgtgtagg cgagcagggc tatggccgca gcccccgcgg cgagcccgtc gaggccgtcc
     7201 gtcaggttga cggcgttggt cgtgccagcg atcaccagca gcaagaacac gctgaagagc
     7261 gcgatcccca tgactccagg ccccaacacc aggctgcggt cgaagtacgg cacgagcacg
     7321 gtctgggtga cccctacgta gcgcacggcg agcacgtccg ccagaacaac cgcgagggtc
     7381 aacagaagga acttgtagcg ggcgctcaac ccctcagggc gctttctaga gatcttctgc
     7441 cagtcgtcgt agaggccgat gccggccacg acggcgacga gcaagagcgt cgtaagcgtg
     7501 gccacgttcg gccgcgctac caccacgagg gccccaagga ggcccgttag catcacaatc
     7561 ccacccatgg tcggggtgcc ggatttgatc aggtgcgtct ggggtccctc ctcgcggacg
     7621 aactgcccga acttcctcga ctgcaagaac tcgatgaact tcgagccgag caacaccgtt
     7681 accagaaacg ccacagcagc cgcgagtaca acgctaaaca agagctaaac tctccctcaa
     7741 cttccgggtc aaggtgtcga gcccaacccc gcgtgaaccc ttgacgatca ggtagtctct
     7801 ggcccgcagc tctccgcgca ggccatcggc ggcgctctct gcgtctgggt agaagagtgt
     7861 cctgccaggg aaggtctctg cataccagcg ggcctcgtcg ccgacacata cgagcagatc
     7921 gacgccgact ctggccgcca gttcacccgc ctccctatgg taagcgcggg cgcaggcccc
     7981 cagctcgaac ataccgccta gcacgacgac gagccgtcta cgttgtctac gtgcctgatc
     8041 cgccccgtac ctgagtacgg cctcgacggc ggcaggggat gcgttgtagg agtcatcgta
     8101 gacggtgatg tcttccctga gattgtagac gtcgccccga aggcccgtac gtcgcagtcg
     8161 ggagagacca cgggcagcct gttccaggcc gaggccgagg gcgagcgtgc cgccgagggc
     8221 tgccaaaagc ggctccacca ggtgtgtccc gaagaccggc gagcgaacct cggccgactc
     8281 tccggctatg ctggcaacaa agcggaggcc ttcgtctctg ccttcgatat cggacgcgac
     8341 gagatctgct tcgccggcgc gggcgaaggt tatacgccga cccagtttac ggtccgaccc
     8401 agtcgccgtc tcgggggcgc cagccggggc gaccagcgta ccagactcgg gtagggagag
     8461 ggctagttcg cctttcgcgg ccgcaaggcc ctcgagcgtc ccgaacgagt ccagatgaac
     8521 cggcgagatg gcggtcagga caccgatgtc gggcggggcg atggtgcaca gagactttat
     8581 gtcccctgcg tgggtggctc ccatctcgag gacgaggacc tctgtgcgag ggtcagaggc
     8641 caggatagtg aggggaagcc cgatctcgtt gttcagattt cctgccgtcg ccgagacacg
     8701 cttcccggcg tgcctgagaa tggtcgcgag tgcgtccttg gtcgtcgtct tgcccacggt
     8761 gccagtgatg cccaca
//
LOCUS       DASTKM010000019         4537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_707424, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000019 DASTKM010000000
VERSION     DASTKM010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707424"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFQ10_00655"
     CDS             complement(<1..416)
                     /locus_tag="VFQ10_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET9963876.1"
                     /translation="MGADLHVAYVDALVSGAQTDSRKVREGDLFFALRGRTDGADFAA
                     EAHLRGAVATVATRRLDVPTLVVDDPLEALQRLARWTLLRQDAPSPAVVGITGTVGKT
                     TTKDALATILRHAGKRVSATAGNLNNEIGLPLTILAS"
     gene            complement(449..2050)
                     /locus_tag="VFQ10_00660"
     CDS             complement(449..2050)
                     /locus_tag="VFQ10_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9963877.1"
                     /translation="MVAAVFAVTVLLLGGRAVHISLTEDASYRAFAEEQAGGTTPAAA
                     PTRGSIVSADGRELATSLEVARVIATPYQIEDPGATARALHALLSEETHTTKKEIRVS
                     LLRRGADGQLSGYSVVATGVEPETAAKVQALGIEGITTVPDTVRVYPDGTLASQLLGH
                     QGDYGEPFGGIEASYDDTLKRGRDVDLTVDSAVQQELQKALAKAVEKSKAKNAVGVVM
                     RVDDGSILALANTPAYDNNQFGDVPAEDQRDRVLTDPYEPGSTFKAFTVASALEEGAV
                     TPSSKFVVPDHMTVAGHVINDSQPHETEILTPAGVLEHSSNVGATKVAKELGGKELYD
                     YIRAFGFGEPTGVDLWGEDPGLVPAYKDWSGVSIGNIPFGQGFTVTPLQLVSGYATLV
                     NGGRRVTPHVTEQETPQGQGPRVISKRTSAIVREMLQGVVDDGSGHFAQISGYSVAGK
                     TGTSQIVDPKTGTYGDQYVASFIGFAPATDPEYVMLVAVDDPQTSYWGELVAVPAFRH
                     VMSFTLGYFNVPPDRRGFEAEEPLW"
     gene            complement(2250..2687)
                     /locus_tag="VFQ10_00665"
     CDS             complement(2250..2687)
                     /locus_tag="VFQ10_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963878.1"
                     /translation="MGASQRRYKATLPTEPAWPVQKRAEDRSQDRTLRRKAAGRRRRF
                     AMLVVVPVLLMLGSVYLNTLAAGLDARAAELGVRLDRAREVGERLEVKVAELSGAERI
                     RALAAEKLGMHEPKSTTLKVYGVRAEDGKNDGAPQEGGRPRQR"
     gene            complement(2687..3622)
                     /gene="rsmH"
                     /locus_tag="VFQ10_00670"
     CDS             complement(2687..3622)
                     /gene="rsmH"
                     /locus_tag="VFQ10_00670"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET9963879.1"
                     /translation="MAAGHRPVMLERAVAALGLAPEDSVVDATFGGGGHSRLILAGLG
                     PGGSLVGIDRDPEAAVRASDLLEDPRFEFVSGAYDLALWEMVEERRSADAILFDLGLS
                     SFQVDEPGRGFSYVNEGPLDMRMDPRSGTTAADFLNTVEVGELSRVLSEYGDVPRPEA
                     RRVAREVVRRRPLLVTTDLYDAVRAAVGWKERGGNPAKRIFQAVRVRVNDELGSLSRA
                     LEASERLLTPGGRLVVITFHSGEDRLVKRFISGREGRCVCPPELPVCVCGAKPVFRRA
                     AVLTPSRREIEENPRSASARLRAAIRTAEPPVDAS"
     gene            complement(3622..4062)
                     /gene="mraZ"
                     /locus_tag="VFQ10_00675"
     CDS             complement(3622..4062)
                     /gene="mraZ"
                     /locus_tag="VFQ10_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET9963880.1"
                     /translation="MALLGEYEHTLDEKGRMTLPSRLRPYFEGGIVITKGVDKCLFAF
                     PPEEWAAFKANIKANADLSARGRQLSRMFFSMAFEATLDRQGRVLIPTKLANYAGLQR
                     DVTITGVDDRLEIWDTEEWDRYVSGADDSFSEIVEEFAGRDLGG"
     gene            complement(4414..4487)
                     /locus_tag="VFQ10_00680"
     tRNA            complement(4414..4487)
                     /locus_tag="VFQ10_00680"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4451..4453),aa:Ile,seq:cat)
BASE COUNT          781 a         1534 c         1360 g          862 t
ORIGIN      
        1 gaggccagga tagtgagggg aagcccgatc tcgttgttca gatttcctgc cgtcgccgag
       61 acacgcttcc cggcgtgcct gagaatggtc gcgagtgcgt ccttggtcgt cgtcttgccc
      121 acggtgccag tgatgcccac aacggcaggc gaaggagcgt cctggcgaag gagcgtccaa
      181 cgggcgagcc tctgcagcgc ttccagcggg tcgtccacta cgagtgtcgg cacgtcgagc
      241 cggcgggtgg ccaccgtcgc caccgcgccg cgcaggtgcg cctcggcggc gaagtccgca
      301 ccatcggtcc tgccgcgcag ggcgaagaac aggtcccctt cgcgcacctt ccgggaatcg
      361 gtctgcgccc cactgacgag ggcgtctaca taggcaacat ggagatccgc gcccattgcc
      421 tgcgcagcct ctaaaagagt tgtcggcctc accagagggg ctcctcggcc tcgaaacccc
      481 tacggtcggg cgggacgttg aagtagccca gcgtaaagct catcacgtgg cggaaggccg
      541 gtaccgctac cagctctccc cagtatgaag tctgcggatc gtctacggca accagcatca
      601 cgtactcagg gtcggtggca ggggcgaacc ctataaagga ggccacgtac tggtcgccgt
      661 aggttcctgt tttggggtcc actatctgag aggtccccgt cttgccggcc acgctgtaac
      721 cggagatctg ggcgaagtgg cccgacccgt cgtcgacgac gccctgcaac atctcgcgta
      781 ctatagccga ggtccttttg cttatcaccc tggggccttg tccctggggg gtctcctgtt
      841 ccgtaacatg cggcgtaact cttcgcccac cgttgacgag cgtggcgtaa cccgagacga
      901 gctgcagagg cgttacggta aagccctgtc cgaacgggat attgccgatc gaaaccccac
      961 tccagtcctt gtaggcgggg acgagccccg ggtcctcacc ccacaggtcc acgccggttg
     1021 gttcgccgaa gccgaaggcg cgaatgtagt cgtagagctc tttcccgccg agctctttgg
     1081 cgaccttcgt cgcaccgacg ttgctggagt gctcgagcac gccagctggg gtcaagatct
     1141 ctgtctcgtg cggctgggaa tcgttgatga cgtggccggc cacggtcatg tgatccggga
     1201 ccacgaactt actcgacggc gtgaccgcgc cctcctcgag cgcagacgcg acggtgaaag
     1261 ccttgaaggt cgagcctggc tcgtacggat cggtaaggac gcgatctcgc tggtcttctg
     1321 cgggaacgtc gccgaactgg ttgttgtcgt aagcaggggt gttggccagc gccaaaatcg
     1381 acccgtcatc aacgcgcatg accacgccga cggcgttctt ggccttgctc ttctcgacgg
     1441 ccttcgccag ggctttttgc aactcctgct gcaccgcgga gtccaccgtc aaatctacgt
     1501 ccctgccacg cttcaaggtg tcgtcgtaac tggcttcgat cccgccgaat ggctcaccgt
     1561 agtctccctg gtgtccgagg agttggctgg cgagggtccc gtctggatag accctgacgg
     1621 tatcggggac ggttgtgatg ccctcgatac ccagggcctg caccttggcc gctgtctccg
     1681 gctcgacgcc tgtggcgacg acgctgtatc cactgagctg gccgtccgca ccacgcctaa
     1741 ggagagagac ccttatctcc ttcttggtcg tgtgagtttc ttcgctcagc aaggcgtgca
     1801 gcgccctggc cgtcgccccc gggtcttcga tctggtatgg ggttgctatc acgcgggcga
     1861 cctcgaggct ggtcgcgagc tcccttccgt cggcactcac tatgctgccg cgggtcggtg
     1921 cggcggccgg ggtcgttcca ccggcctgct cctcggcgaa ggccctgtaa ctcgcgtcct
     1981 ccgtgaggct tatgtgcacg gccctcccac cgagaagaag taccgtcacc gcgaacaccg
     2041 ccgccaccat ccacagcctc ctatggccca cggtagtcct agcttcgggg gccatcgacg
     2101 acgcgcgacg cgccctggtc gcgccgctgg cccgcctctg gccgcggagc ggtatgactt
     2161 tgccaccaga ggacttaggc cctttgtttt tggtgcgagg gggggtcttc ttgccacggc
     2221 gaatccttgc gttgcgaggc cctggccgat caacgctgcc gcggtcttcc cccttcctgt
     2281 ggtgccccat cgtttttccc atcctccgct cttacgccat agactttcaa ggtagtgctc
     2341 ttcggctcgt gcataccaag cttctccgcc gcgagggccc ggatcctctc cgcacccgac
     2401 agttcggcga ctttgacctc gagtctctcc cctacttctc tggccctgtc aagcctgacg
     2461 ccgagttcgg ccgccctggc gtcgagcccc gcggcgagcg tgttcagata gacgctcccc
     2521 aacatcagca gcacgggcac cacgaccagc atcgcaaacc ggcgacgccg gcccgcggcc
     2581 ttccgcctca gggtgcgatc ctgcgagcgg tcctcggcgc gcttctgtac cggccacgcc
     2641 ggttcggtag ggagcgtagc cttgtacctg cgctgggagg cgcccatcag ctcgcatcca
     2701 ccggaggctc ggccgtcctt atcgccgccc gcagcctggc cgatgcgctg cgcgggttct
     2761 cctctatctc acgccgggaa ggggtcagaa ccgccgctcg acggaagacg ggcttcgcac
     2821 cacaaacgca caccggcagc tccggcggac agacacaccg cccctcccgc cctgagatga
     2881 atcgctttac gaggcgatcc tcgcccgagt ggaacgtgat gaccacgagc ctcccacctg
     2941 gtgtgagtag ccgttctgaa gcctccaggg cccggctgag gctgcccaac tcgtcgttta
     3001 ccctgacccg cacagcctgg aagatcctct ttgcagggtt accgcccctc tccttccagc
     3061 ccacggccgc acgcacggcg tcgtaaaggt ccgtcgtaac caggagcggc ctccgccgaa
     3121 cgacttcccg cgccacacgc cttgcctcag ggcgcggcac gtccccgtac tccgagagca
     3181 ccctagagag ctccccgacc tcgacggtgt tcaagaagtc cgcggccgtc gtacccgacc
     3241 gtgggtccat ccgcatgtcc agtggtccct cattgacata tgagaacccg cgtcctggct
     3301 cgtcgacctg gaagctggag aggccgaggt cgaacaggat cgcatcggca cttcgacgtt
     3361 cctcgaccat ctcccacaac gccaggtcgt aggcgcccga gacgaactcg aagcgcgggt
     3421 cttccaacag atcagaggct cgcactgccg cctcggggtc gcggtcgatg ccgaccaggc
     3481 taccacccgg cccaagacca gcgagtatga gcctggagtg gccgccaccg ccgaaggtgg
     3541 cgtctacgac ggagtcttcg ggagccagcc ccagggcggc caccgctctc tcgagcatca
     3601 caggacgatg tccggccgcc atcacccgcc gagatccctt cctgcgaact cctcgacgat
     3661 ctcggagaac gagtcgtcag cacccgagac gtagcgatcc cattcctccg tgtcccagat
     3721 ctccaaccgg tcgtccacgc ccgtaatggt cacatcacgc tgtagtcccg cgtagttggc
     3781 gagcttggtt gggatcaaaa cccgaccctg cctgtctagc gtggcctcga aagccatgga
     3841 gaagaacatc cgcgagagct ggcgccccct ggccgagagg tccgcattgg cttttatatt
     3901 ggccttaaac gccgcccact cctcaggcgg aaacgcaaag aggcacttgt ctactccctt
     3961 ggtaatgacg attccaccct cgaaataagg tcggagacgg gaagggaggg tcatgcgacc
     4021 cttctcgtcc agcgtgtgct cgtactcacc taacagggcc agcgccgtgt cctcccacta
     4081 cgctccactt tgacccacga cgacgaaatt cttgcatatg ctgggaaaaa tcgcaatcta
     4141 ggagggtttt cgggcccaaa gagagccaat tttcgacctt cagaccactt ctatccctcc
     4201 tccccagggg tgaggacccc tgctagatta ccgtattggg caggcggtgg cggtagcatc
     4261 tagccaggag gtagtgtgga cggttgcccc acatatcggg gctatgttgc ccagggctca
     4321 acatgttggg ctgcactgaa aaagtgttgg tgggaaagaa cgtttggtgt ggtgatggga
     4381 cctctggagc tgcaacgcgg ggtaatcgat acgtgggccc accaggactc gaacctggga
     4441 ccgtccgatt atgagtcgga tgctctaacc aactgagcta tgggcccttg gcgggaaacc
     4501 cggacgggac cgcccgctcc cccggctgga ctcgaac
//
LOCUS       DASTKM010000020         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_883740, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000020 DASTKM010000000
VERSION     DASTKM010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883740"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..1554
                     /locus_tag="VFQ10_00685"
     CDS             328..1554
                     /locus_tag="VFQ10_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9963881.1"
                     /translation="MKVLVVGAGPAGVATALLLARYGVDVKLVERERSFERVFRGEGL
                     MPLGIDALFEMGLGGVLQSVPGRRVDSWDIWIDGEEVFVIPEPVEELGERAMRVVSQP
                     ALLEKLVEEASGCPSFSFERGARFRDLLHDRDGRVVGAALEKGEGREEVRADLVIGCD
                     GRGSLVRKRAGLVLELLPEQYDVLWFKLPTPDRLREGCSIMIAVAAKEHPALCYTSWD
                     GRLQYGLVMPKGGLKDIREKDWVSNAVKSAPSWLADHVVAHQDEIEGPSRLNVLVGRA
                     PEWAAPGVLLLGDAAHPMSPVRAQGINLALRDAVVTANHLVPVLREDGEPKVLDAACR
                     AVKAEREPEIARAQKLQRREAAGQGHARSGSRRFELTKRLARRMGRNRWAQAAWLKRQ
                     HDLRFGSKEVKLKVPV"
     gene            1641..2129
                     /locus_tag="VFQ10_00690"
     CDS             1641..2129
                     /locus_tag="VFQ10_00690"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET9963882.1"
                     /translation="MSGSLFNPRRNKPDAGEGTPVAAVIMGANLLNHFAALLVASEPY
                     YAATFGTESSEALVLLFAEVHAQGDLIGGIFFRLWLLPLGYLVYRTGYFPRVLGIMLM
                     VGCFGYLTDAFVSSLFPSVGASVGLLFRSVAGIAEISFLLWLLIMGAKVPARDDNINT
                     AV"
BASE COUNT          463 a          756 c          881 g          465 t
ORIGIN      
        1 atggtgtcga tacatacaca agataatgag ataaggtcag cggttcgagt ccgctcgtcg
       61 gcgctcggta agctcgatcg atgagctaca aacttgggat aggaaaagcc ggcggttcgt
      121 gactgggggc ttctgacacc aaagtgctcc agttctctgt cacgaagtgc gcgagcccgc
      181 gcatgcgccg caaggacgga cgaccggagc gcgaattcgg gttagccgtg ggatccagcg
      241 acgaacccct gtaggcggcg cccttcccgg tcgaggacac tagggtagat tttcggcgag
      301 acgaccgtac cgatgggagg ttgccgggtg aaagttctag tggtaggcgc cggtccggcc
      361 ggggtggcga cggcgctgct gctggcgcgc tacggagtgg acgtgaagct cgtcgagcgc
      421 gagaggtcgt tcgagcgggt gttccgcggg gagggactca tgcccttggg gatcgatgcc
      481 ttgttcgaga tggggctcgg gggagttctc cagagtgtgc cgggtcgcag ggtggactcg
      541 tgggacatct ggatcgacgg tgaggaggtg ttcgttattc ccgagcccgt tgaggaactc
      601 ggcgagcgcg ccatgcgcgt cgtgtctcag ccggccctcc tcgagaagtt ggtcgaggag
      661 gcgagcgggt gcccgtcatt ctccttcgag cggggagcgc ggtttcgcga cctcctccac
      721 gaccgggacg gccgtgtcgt cggagcggca ctggagaagg gggaagggcg ggaagaggtg
      781 cgggcagacc tcgtcatcgg ctgcgacggg cgcggatcgc tcgtgcgcaa gcgcgcggga
      841 ctggtgctgg agctgttgcc cgagcagtac gacgtgttgt ggttcaagct gccgacgccc
      901 gatcgcctaa gggaagggtg ctctatcatg atcgcggtag cagcgaagga gcatccggcc
      961 ctctgctata cctcctggga cgggcgcctg caatacgggc tggtcatgcc caagggcggc
     1021 ctcaaggata tccgcgagaa ggactgggtt agcaacgcgg tgaagtcggc gccttcttgg
     1081 ctcgccgacc atgtcgtcgc acatcaagac gagatcgagg gccccagcag gctcaacgtg
     1141 ctcgtaggcc gcgcccctga gtgggcggca ccgggggtat tacttctcgg cgacgcagcg
     1201 caccctatga gcccggtgcg ggcccagggc atcaacctcg cgctgaggga cgctgtggtg
     1261 actgccaacc atctcgtccc ggttcttcgg gaggacggcg agccgaaggt gctggacgcc
     1321 gcgtgccgcg ccgtgaaggc cgagcgtgag cccgagatcg cgcgcgccca gaagctacag
     1381 cgtcgcgagg ccgccgggca ggggcacgct cgctcaggga gcaggcgctt cgagttgacc
     1441 aagcgcctag cccgacggat ggggcgcaac cgatgggctc aggctgcctg gctcaaacgc
     1501 caacacgatc tgcgattcgg ttcgaaggag gtcaagctca aagttcctgt ctgatcgaca
     1561 tgcaacgaac ggcgggtagc tctatggccg aaaagtccaa cccggcacaa cttgagagcc
     1621 gggtcctcgt gccctagagg gtgagcggca gcctcttcaa tccgcgcagg aacaagcctg
     1681 atgccggcga agggactccc gtcgcggcgg tgatcatggg cgccaacctg ctcaaccact
     1741 ttgcggcgct gctggtggcg tccgaaccct actacgcggc gacgttcgga accgagtcgt
     1801 ccgaagcgct cgtgctgctt ttcgccgagg tgcacgcaca aggggacctg atcggtggga
     1861 tcttcttcag actgtggctg ctcccgctag gatacctggt ctacaggacc ggctacttcc
     1921 cccgcgtgct ggggatcatg ctgatggtcg gctgcttcgg ttacctgaca gacgcgttcg
     1981 tgtcctcgct cttcccgagc gtcggcgcaa gcgttggcct gctcttccgc tcggtggcgg
     2041 gcatagcgga gatctcgttc ttgctgtggc tgctcatcat gggcgcgaag gttcccgcgc
     2101 gggacgacaa tatcaacaca gcggtttagg ccgagttcct accgaaaggg catgccaagg
     2161 gaatgccatg accactcacg caaatggcgc cacagacacg ataaaggcca gagtgggccg
     2221 aggccaacgc gacccgcccg gcagcctgag aacatttaga gtggctctca ggctgtccga
     2281 ggccctgaga tgcgtagcgc ccaggcctat tcacggaccg tcgtagagct cgactttagc
     2341 catttgagag cggaaggggt agggatactc ctaacccatc cactcaaaga gagtgcggat
     2401 ctcaaggcac ctgccccctg ctgttctcgt cttggcgacg atatggtcac agcgctccga
     2461 aatgggctac tctgaggctt ctagcccaat ccccttaagg gggatgccct ttgggcactg
     2521 tgtccactcc tgcgcctact cctcctcgtg cgcgcaagga gcagg
//
LOCUS       DASTKM010000021        13153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1412331, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000021 DASTKM010000000
VERSION     DASTKM010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13153
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412331"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFQ10_00695"
     CDS             complement(<1..652)
                     /locus_tag="VFQ10_00695"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET9963883.1"
                     /translation="MLRTAAPVYGLAVVVAFALFPALAAAQGSDTVYDEAGALSGPEE
                     QRVQQAFDSAQEETGQPLYAFLVPNKGVEGQEARQDLLTREATEAQVPQDAGVVVVAT
                     NDGWGTTYNFPQDAYNTMVPDFQEGDFAAGLVAGAREIQGEPAAQGAQNDPDGSTGGL
                     VGGGGLLLLLVAVAGALLLFRKRRANKRRIEEERRSAEEEFANLTVHLNEFDEKERL"
     gene            1042..1890
                     /locus_tag="VFQ10_00700"
     CDS             1042..1890
                     /locus_tag="VFQ10_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963884.1"
                     /translation="MDGQRARPLMRGIAAIALTMSLAVAGACGAGTTEAKASAVKDQS
                     PGRPARAASSTAAGSGSRGGMPQGSEPVKLDPADFSVSIDNPYWPMSPGTKWVYSETD
                     TKGTNQKVVVEVTDETKMMANGIEARVVRDTVTENGAPVEITDDWYAQDKAGNVWYLG
                     EYVTNYENGKAIDHGGSFEAGVDGAQPGIAMPANPDPGLSYRQEYYKGEAEDKAAIIT
                     IGEERVQVPLGYFDKDVLMTRDLVPTEPRVQELKFYAPNVGPLLSMHTDGDGSRAELI
                     SYSRDG"
     gene            complement(1997..2266)
                     /locus_tag="VFQ10_00705"
     CDS             complement(1997..2266)
                     /locus_tag="VFQ10_00705"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HET9963885.1"
                     /translation="MCRNIKTLFNFAPPATDEEINAASLQYVKKVTGFNKPSKTNETA
                     FYSAVEDIARISERLLTSLETTAPPKDREEEAAKAKARSALRFGG"
     gene            2855..3667
                     /locus_tag="VFQ10_00710"
     CDS             2855..3667
                     /locus_tag="VFQ10_00710"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET9963886.1"
                     /translation="MNLRQPSAPALGGLIARLQQRLNARATAATREWWTKYLRGAASF
                     RGVKMEDIRTAVHAWFEEEQLGEYLSVGQQKDLALMLLEDEHTEDKLAGVLFLQEILL
                     PEGALDWRSELPRFARLFDAGYISDWSICDWLCVKVLGPLVGQQGESCARAISEWREA
                     DSLWQRRASVVAFANLARKGDQNFPGFTEMVLDNCSHLLGSQERFVQTGVGWVLRELG
                     RTDQDRVIGFVEANLERFSREALKNATKYLPPEVAERLLQTHLPSGNRSRRR"
     gene            complement(3809..5020)
                     /locus_tag="VFQ10_00715"
     CDS             complement(3809..5020)
                     /locus_tag="VFQ10_00715"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET9963887.1"
                     /translation="MLRFGRDSRSAQPWLRPPRLRTLADAEEERHASWLELFFDLVFV
                     VAIAELSHELVVNHTLNGFAIFAALFVPVFVAWQGFSFYADRFDTDDVFVRVCMLAAM
                     LAVAALAIQIPSVAHGESSAGFALAYIAIRVLTVVLNLRAYRHVPLARPLTGRYVAAF
                     SFSVALWTVSLAVPEPWRFLLWGIGLAVDLGVPALVGPRFVGRVPLHASHIPERFALF
                     TIIVLGESVVAVALGVAGSDWELSSAAGAALGFLAVACLWWTYFDVGAGLALAPGPWT
                     AVRFIYVHVPMWAALTAVAAGVNLLIEEAGTGSLEAGARWALCGGAAVYLCCTSVIQD
                     ATARGILRPILGLRLSVVGLLVALAFLGAGLRPVTFAAVMVALLVALVAFEISRNLHP
                     DAHRRAKPSAD"
     gene            5608..6441
                     /locus_tag="VFQ10_00720"
     CDS             5608..6441
                     /locus_tag="VFQ10_00720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9963888.1"
                     /translation="MANALVSPHIFEIAFDALTAAVGGLVILLALRVAPGLTLSSHRL
                     ALRISIVAAVLITTAQLAEVLADFSRLSTIEDAAADVAELIAVCFVGLALHQIGRAEK
                     GEISSLRRAANVDHLTGLVSRSYFHRAAERRIELYKRNGLPLACAVLDVDNFKSYNDR
                     YGHGSGDEALCCVGRVLRESTRADDVVARYGGEEFVVLMGGNIEDAIEVAERVRHGVE
                     HESATENESLLGSSVTVSVGVAALTEDVSSLEQLVEKADDELYRAKRAGKNRVAAAGR
                     R"
     gene            6570..6824
                     /locus_tag="VFQ10_00725"
     CDS             6570..6824
                     /locus_tag="VFQ10_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9963889.1"
                     /translation="MSPVAIVTASDSGIGQESAKELAENGFDLGITYRTDEAGAQETL
                     EAVRAAGRRGEVRRLDRRRGRGPDDHRTFESAARTPRTRT"
     gene            complement(6933..7211)
                     /locus_tag="VFQ10_00730"
     CDS             complement(6933..7211)
                     /locus_tag="VFQ10_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963890.1"
                     /translation="MLSRETIIHGFGSVRSDQEKVRSSVQADSLRRGRMSTASAERTN
                     ADVIGASISRRGDGIATGHTIVGRPNYWDEYAHGELDELEGGPGALIF"
     gene            7238..8371
                     /locus_tag="VFQ10_00735"
     CDS             7238..8371
                     /locus_tag="VFQ10_00735"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET9963891.1"
                     /translation="MYSENRFTTMNRRDFLRLGGAGLAGATLLGTAGGRVLAQTDSSL
                     KAKFETAARKYKVPVELLLAMGYYNTLWEMPPPSASAYRKGDPEGRGDYGIMQLTQNP
                     SRNTLGKAAKLTGLSKARLKNDRSANIEGGAAFLSHLVGKTKPKRLDGWQEALTQYAD
                     TDLYASQVYGVLREGASTTISTGEHLKLSPQNVEVPQVYTAQSGATDYPQALWRPAAS
                     CNYTNSNRETTYDVRKIVIHVAEGSYSGTLSWFENCAAQASTHYVVSSKGRIAQCVRD
                     EDIAWHAGWWDTNTHSIGIEHAGYIDNPEWLTRSMYHASARLSAWCCKKYKIPIDSKH
                     IIGHYQVPGCSGSGGGVDCHTCPGPYWNWNKYMRLINYYRSLL"
     gene            complement(8430..10106)
                     /locus_tag="VFQ10_00740"
     CDS             complement(8430..10106)
                     /locus_tag="VFQ10_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9963892.1"
                     /translation="MKDRMDEIRSTRAPRLREDQIEILRRYGQKRKTEVGDVLFRAGE
                     TQNDFIVILEGQVEVVDDFAYGARTIGVFLAGRFLGELNMLTGQAMYLTAVVREGGEV
                     LAITRERLKEIVTEEPNLSDVILKAFLARRTYMIRTGLGLRIIGSRHSSDATRLREVA
                     ARNRVPHVWIELVENPGAEALLERFGAKPSETPVTIWQGEDVLKNPTNAEFARTTGLE
                     VDAPLDRTYDLVVVGTGPAGLGAAVYGASEGLSTLALESIALGGQAGTSSRIENYPGF
                     PAGLSGFELASRVLVQADKFGARTAVPREALGLRRENGHYYIQLSEGGEVAARSVIVA
                     TGARYRRLEVPRLERFEGVSVHYAATEAEAQRCEGEEVAIVGGGNSAGQAALFLAGRT
                     RRVYLLIRGEDLGKNMSRYLVDRITNAENIELLVSTGVRELTGEDHLKGVVIEDNRSG
                     ARRTLGARALFVFIGAEANTGWLQGAVELDERGFVLTGRELNDSALHEEDWQERSREP
                     FLLETSMPGVFAAGDVRSGSIKRCASAVGEGSMAVRLVHQHLADAGVQKV"
     gene            10309..11448
                     /locus_tag="VFQ10_00745"
     CDS             10309..11448
                     /locus_tag="VFQ10_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET9963893.1"
                     /translation="MKITGVETYPVWGGERNYLFVVVDTDEDLYGVGEAGLTGRELAV
                     TGTIEHFEPLLLGENPFRTEHLWQLLFRGGFFPARHAVSAAISAVDIALWDIKGKALG
                     VPLYELLGGRARDRVVCYPHNAEAGTDTAALVESCLQTTDEGWRFVRWGLPQEGEVFE
                     PRRSTLAAVEQLAAVRDAVGEEIEMILDVHTRLEMPDSLRLCREAEPLRPYFIEDPLR
                     SEDPRSYELLRLRTAVPLAAGEQFGSKWEFRELVEREWIDYARVDPCIAGGITEAKKI
                     AAMCETHHVKLAVHNPLGPVSTATCLHLNLSCPNFGVQEQPYRPGTFLTDVVPVQPKW
                     EDGHLLPPTRPGLGVEFDREAAKGRPFRMAELPHLRRPDVSVTNW"
     gene            12161..12310
                     /locus_tag="VFQ10_00750"
     CDS             12161..12310
                     /locus_tag="VFQ10_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963894.1"
                     /translation="MERMRKQELSGGLGSREVSTPRLTTPLLESGPEEGVPVVFVHGC
                     GTLRT"
     gene            complement(12444..12578)
                     /locus_tag="VFQ10_00755"
     CDS             complement(12444..12578)
                     /locus_tag="VFQ10_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963895.1"
                     /translation="MGAGLVSGREVDNVVAELGRLADDDRTLMSIAPTFQVWARRAGA
                     "
BASE COUNT         2584 a         4100 c         4065 g         2404 t
ORIGIN      
        1 cgagccgctc cttctcgtcg aactcgttca ggtggacagt gaggttggcg aactcttcct
       61 cagcgctccg acgttcttct tcgatgcgcc gtttgttggc gcgccgcttc ctgaacaata
      121 ggagagctcc tgctaccgcc accaatagaa ggagtagacc gcctcccccc accagaccgc
      181 ccgtactacc atcaggatcg ttttgcgcgc cctgtgccgc aggctcaccc tgaatctctc
      241 gtgctccggc cacgagccca gcggcaaagt ctccctcctg aaagtccggt accattgtat
      301 tgtatgcgtc ctgcgggaaa ttgtacgtcg tgccccagcc atcgttcgtg gcaaccacga
      361 ctaccccggc atcctgcggg acttgcgcct cggtggcttc cctggtcagt aggtcctgcc
      421 gcgcctcctg gccttccacc cccttgttcg gtaccaggaa ggcgtagagc ggctgccccg
      481 tctcctcttg cgccgagtcg aaggcttgct gtacgcgctg ctcttccggc ccgctaagtg
      541 ctccggcctc gtcgtacacc gtatccgagc cttgggccgc cgcgagcgcg gggaagagcg
      601 cgaacgcgac aacgaccgca agtccataca caggcgccgc tgttcttagc acaccgatca
      661 actcatggaa gattataccc aggcgggtag tcgccgaagg tttaggggtc ggctatcgcg
      721 agaaacccgt cctggctagt gctcgcccgc gaactaagag gcgacgcagg tggcgctcat
      781 gagcaacgac acgcccggag cggtcgctcg aaccctttct cggacggttt tcgaaggtca
      841 aactaggatg ttcacaggaa tctaatcctg ctctcagcgt gcgctcaacc tgtcctcacg
      901 ttcgcttgcc atgctgcttt cagcttcacg tgcacgaagg tctgtgagag cggctgatag
      961 aagagccgac gaacgaggaa gggtaggaaa agtcgccgcg cgcggacgac tgcgaacggc
     1021 agagatcgaa gaggaggaac gatggatggg cagcgtgcga gacccctgat gcgtggtata
     1081 gcggcaatcg cgctgacgat gagtctggca gtggcgggcg cgtgtggggc cggtacgaca
     1141 gaagcgaagg cttctgcagt gaaggaccag agccccggga ggccggccag ggcagcaagc
     1201 tcgaccgccg cgggatctgg cagccgggga ggcatgccgc aaggaagcga gcccgtaaag
     1261 ctcgacccgg ccgacttctc ggtcagcatc gacaatccct actggccgat gagcccggga
     1321 accaagtggg tctacagcga gacggacacg aagggcacga accagaaggt cgtcgtcgag
     1381 gtcaccgacg agacgaagat gatggccaac gggatcgagg cccgcgtggt acgcgacacg
     1441 gtcaccgaga acggcgcgcc ggtcgaaatc accgacgact ggtatgctca ggacaaggcc
     1501 gggaacgtct ggtacctcgg cgagtacgtc accaactacg agaacggcaa ggccatcgac
     1561 cacggcggtt cattcgaggc tggtgtcgac ggggcgcaac cggggatcgc catgccggcc
     1621 aaccccgacc ccggcctctc ctaccgccag gagtactaca aaggcgaagc cgaggacaag
     1681 gccgcgatca tcaccatcgg tgaggaaagg gtccaggtgc ccctcggcta cttcgacaag
     1741 gacgtcctga tgacgcgaga cctggtaccg acggagccga gggtccagga gttgaagttc
     1801 tacgccccga acgtcggccc actgttgagc atgcacacgg atggcgacgg cagccgcgca
     1861 gaactgatca gctactcccg cgacggatag ccagagttcg gcacgatacc acttggggag
     1921 gcagaggcgg aaaccgtggt cgcaatagac tcacgcctta tgcagcgctc gtaccggcgg
     1981 gttaccacgt cgcgcatcac ccgccgaatc gtagtgctga gcgtgctttc gctttggctg
     2041 cctcttcctc cctgtcctta ggcggtgcag tggtttcgag cgaggtgagc aaacgctctg
     2101 agattctcgc gatgtcttcg actgcggaat agaaggccgt ctcgtttgtc tttgacggct
     2161 tgttgaagcc agttaccttc ttcacgtact gcagcgaagc agcatttatc tcctcatcgg
     2221 ttgccggtgg cgcgaagttg aaaagggtct tgatgttcct acacatagta ctcggtctcc
     2281 agactaggag ccccaccgca ggactcgttt gctatcagca tgtcttctgt gctaagtgcg
     2341 ctgaatacga gcttcgagtt cataggttct cctcttgttg tccctaccga cttggcttgt
     2401 tcgacggccc gatctcctcg gccagctcct cgagactctc cagagttcga ccggtgagga
     2461 cgaccttggc cccttcgcag gcgaaggcaa cagcaccagc tacctttcat ccgtctccgt
     2521 agatgacggc gttcttatcc tcgagcagca ctctcgtcct ccctgtctct cgggttgcta
     2581 gcacctagtc aaagcatacg ccaacagtcc taaatatgaa gtagcaagat aacagctagc
     2641 cacctgctgt tgcacaggca gggaccacat ccgacagcgt gttagcaaaa caggtaacct
     2701 ggagggacgt agagttggcc gacaagcagt gggagaggct gaagcctgtg ttcgctccac
     2761 agaagcccag gaccggccgc cccgctcacg agcatcacat ggtcgtggat gacatcctgt
     2821 aggtgctgct ccgaccagtt ggagtgaacg atctatgaac cttcgccaac cttccgcccc
     2881 agcactcggc ggtctaatcg ccaggctgca gcaacggttg aatgcgcgcg caacggcagc
     2941 cacgcgtgag tggtggacga agtacctcag aggcgctgcc tccttccgcg gcgtcaagat
     3001 ggaagatatc cggacggctg tgcatgcctg gttcgaggag gagcaattgg gagagtacct
     3061 ctccgttgga cagcagaagg atctggccct gatgctgctc gaagacgagc acacggagga
     3121 caagctcgca ggcgtcctct tcctgcagga gatccttctg ccagaaggcg cactggactg
     3181 gcgctccgag ctgccccggt tcgcgcgcct ctttgatgct gggtatatca gcgactggag
     3241 catctgcgac tggttgtgtg taaaggtgct cggccccctg gtagggcagc agggcgagtc
     3301 gtgcgcacga gccatctcag agtggcggga ggcagacagc ctctggcagc gtcgcgcctc
     3361 cgtcgtggcg ttcgccaatc tggccaggaa gggtgatcag aacttcccgg gctttactga
     3421 gatggtgctc gacaactgca gccatctttt aggaagccag gagaggttcg tccagacggg
     3481 cgtgggctgg gtcctgaggg agctcggccg cacagatcag gaccgcgtaa tcgggttcgt
     3541 cgaagctaac ctggaacgct tctctcggga agctttgaag aacgcgacca aatacttgcc
     3601 acccgaggtg gcggagcgtc tccttcagac gcaccttccg agtggcaatc gcagtcgtcg
     3661 gagatgagca actctgtgga tgctgtcacc tgctcgtgcg acgaacttgc ggctggggcc
     3721 tgccaagcaa catcgcccaa gctgcccttg caaatacgtc cctggcttct cctctggggg
     3781 tcggaacgct ctttccagag agtcgttgtt agtcagcgga tggcttggcc cggcgatgag
     3841 catccgggtg cagattgcgc gagatctcaa atgcgacgag cgcgacgagg agagccacca
     3901 tcactgcagc gaacgtaacc gggcggaggc cggctccgag gaaagcgagc gctacgagca
     3961 ggcctacgac gctcagacgc aagccgagga tcggccggag aataccccgc gccgttgcgt
     4021 cctgaataac gcttgtgcag cacaggtaca ccgccgcgcc gccgcaaagc gcccatcggg
     4081 cgcccgcttc gagtgacccc gtacctgctt cctcgatgag gaggttcacg cccgctgcaa
     4141 cggccgtcag agcggcccac attggcacgt ggacgtagat gaagcgaaca gcggtccacg
     4201 ggccaggcgc caaggccaag cccgcgccga cgtcgaagta cgtccaccaa aggcatgcca
     4261 cggcgaggaa acccaaggcc gcgccggccg cagacgacaa ctcccaatcg ctgccggcca
     4321 cgcccaaagc cacggcgact accgattcgc ccaagacgat tatcgtaaac agtgcgaagc
     4381 gctcggggat gtggctcgcg tggagtggca cgcgtccgac gaagcgcgga ccgaccaggg
     4441 cgggaactcc caggtcgacc gccagcccga tcccccacag cagaaaacgc cagggctcgg
     4501 gaacggctaa cgagacggtc cacagcgcga cgctgaagct gaacgccgcc acgtagcggc
     4561 cggtgagagg acgtgcaagg ggcacgtggc ggtaggcgcg caggttgagg accaccgtta
     4621 gaaccctgat ggcgatgtag gcgagggcaa agcctgcgct gctctccccg tgcgcgacgc
     4681 tagggatctg tatggcgagc gcggcgacgg cgagcatcgc ggccaacatg cacactcgga
     4741 cgaatacgtc gtcggtgtcg aagcggtcgg cgtagaaact aaaaccctgc caggctacga
     4801 agacgggcac gaagagcgcc gcgaagatcg caaagccatt cagtgtgtgg ttcacgacga
     4861 gttcgtgtga gagttcggcg atggcaacga cgaacaccag gtcgaagaag agctcgagcc
     4921 aggaggcgtg gcgctcctcc tcggcgtcgg cgagcgttcg gaggcggggc gggcgtaacc
     4981 agggttgggc ggatcgggag tcgcggccga agcgcagcat cgactaggcc cccaccgatc
     5041 gggcacgacc aaagggagcg aagctgagga tctcgagtcc tcttctagtc gcgagatccg
     5101 cgtgtagccg tgcgttttgc accgccacat gatacctcta ccagattgtt aatgaacaca
     5161 gtaagagtcc tgtcgtcact atcatctgct gcacggagga gatgctgggg cgttcgcgta
     5221 gactggccgt ataatctcgg aatggctcca agcgatgaga acgcagggcg tcgcacagct
     5281 ggcaggtgcc agctgtgcgg cagcctcatc ggagaggatc gactcacccg ccaccatctc
     5341 ttgccgcgct ctcgctctag gaagatgaag cgaagaagga agggtaggca agagctcaag
     5401 cgacgtgacc cgagcacgac ggtcgccctg tgcggcccat gtcacagaaa cgtacacgtc
     5461 tctatcggca acgcagacct cgcacgaggc tatgattccg tggaggcgct tcgagaccat
     5521 cccggagtgc ggcggttcac agagtgggtg aaggacaagc cgcacggtcg tgcctgagca
     5581 gtagctcgca gatgatatgg agatagtatg gccaacgcgc tagtctcgcc gcacatcttc
     5641 gagatcgcct tcgacgcgct gaccgccgct gtgggaggcc tcgtaatcct tttggcgctg
     5701 cgagtggcgc ccgggctgac actctcctcc caccggctcg ccctacggat ctcgatagtc
     5761 gctgcagtcc tgatcacaac cgcacaattg gcggaggtgt tggcggattt ctctcgacta
     5821 agcacgatag aggacgccgc cgcagacgtc gccgaactga tcgccgtatg ttttgtaggt
     5881 ttggccttgc accagatagg ccgggcggag aaaggggaaa tctcctcatt gaggagggcg
     5941 gccaacgtgg atcacctgac cggcctggtc agtcgatcct acttccaccg ggcggcggag
     6001 cggcggatag aactctataa gagaaacggt ctgcccctcg cctgcgccgt gcttgatgtg
     6061 gacaacttca agtcgtacaa cgaccgctac gggcacggga gcggggacga ggccctgtgc
     6121 tgcgtaggac gcgtgttgcg tgagtcgacg cgggcggacg acgtggtggc ccgctacggg
     6181 ggcgaagagt tcgttgtttt gatgggcggc aatatagaag acgccatcga ggtcgccgaa
     6241 cgcgtacgcc acggagtcga gcacgagagc gcaaccgaga atgaatccct tctgggtagt
     6301 tccgtaacgg tctcggtggg cgtcgctgct cttaccgagg acgtgtccag cctggagcag
     6361 ctcgtcgaga aggcagacga tgagctgtac cgggccaaga gagcgggcaa gaaccgggtg
     6421 gccgccgcgg ggagacggta agacgagttc gggtccaccc tcacgtgcgc gtcgagccag
     6481 acgccgtgca cgcgactcta ttctgtttgt ggtctccact ccggggcata gagccgggtg
     6541 ggcaggtcct gtggacgaga ggagagagca tgagcccggt agccatagtt accgcatcgg
     6601 actcggggat cgggcaggag tcggcgaagg aactggcgga gaacggcttc gacctcggca
     6661 tcacctaccg tacggacgaa gcaggggcac aggagaccct tgaggccgtg cgggcggcgg
     6721 gccgcagggg cgaggtgcga aggctggacc ggcgccgagg acgaggaccc gacgaccata
     6781 gaacgttcga atctgccgct aggacgcccc ggacacgcac gtgagatcac agcgtgggtg
     6841 gccttcctcg cctcggagaa gtcaacgtac gcaaccggcg cctcgttcgt cgtggacggt
     6901 ggcctgacgc tgatggctgc agagttgtcg agctagaaga tcaacgcccc aggaccacct
     6961 tcgagctcgt cgagttcgcc gtgggcgtac tcgtcccagt agttaggcct gccgacgatg
     7021 gtgtggcctg tggcgatccc gtcaccgcgg cggcttatag aggccccgat cacgtcggcg
     7081 ttcgtccttt cggcagacgc cgtgctcatg cggccgcgcc ggaggctatc agcctgcacg
     7141 ctcgagcgca ccttctcctg atcgctgcga acagatccaa aaccgtggat aatggtctct
     7201 ctggacagca cctgagagag cgccaacgga gggggaaatg tattctgaga accggttcac
     7261 gactatgaac cgccgagatt tcctgagatt gggcggagca ggccttgcgg gcgccacctt
     7321 gctcggaacg gcgggcggca gagttctggc acagaccgac tcttctctga aggcaaagtt
     7381 cgagaccgca gccagaaagt acaaggttcc ggtcgagttg ctcctggcga tgggctacta
     7441 caacaccctc tgggagatgc ctccaccgtc cgccagcgcc taccggaagg gagatcccga
     7501 aggtcgcggc gactacggga tcatgcagct cacccaaaac ccgtctagga acactctagg
     7561 caaggccgcg aagctaactg gtctctccaa agctcgactc aagaacgatc gctctgccaa
     7621 catcgagggc ggcgcggcct tcctttcaca cctggtcgga aagaccaagc cgaagagact
     7681 ggacggctgg caggaggcgc tcacccagta cgcggacacg gacctgtacg ccagccaggt
     7741 ctacggggtg ctcagggaag gggcctcaac gaccatctcg accggtgagc acctgaagct
     7801 ctcgccacaa aacgtcgaag taccccaggt ctacaccgcc cagagcggcg ccaccgacta
     7861 cccacaggcc ttatggcgtc ctgccgcttc atgcaactac accaactcca accgggagac
     7921 cacctacgat gtaaggaaga tcgttatcca cgtcgcagag ggttcctact ccggcaccct
     7981 aagctggttc gagaactgcg ccgcacaggc gtcgacgcac tacgtcgtca gcagtaaagg
     8041 taggatagcg cagtgtgtgc gcgacgagga catcgcctgg cacgctggtt ggtgggacac
     8101 caacacacac agcatcggca tcgaacacgc gggttacata gacaacccgg agtggttaac
     8161 cagaagtatg taccatgctt cggcgaggct ttcggcgtgg tgctgcaaga agtacaagat
     8221 ccccatagac agcaagcaca taatcggcca ctatcaagta cccggctgtt ccggctccgg
     8281 cggcggggtc gattgccata cctgtccggg cccctactgg aactggaata agtacatgcg
     8341 cctcatcaac tactatcgga gtcttctcta gcgctatccg cgggcagatc tacgtaggag
     8401 gccctcgagg gcggtgaggg gcggaatcct cacacttttt gcacgccggc atcggccagg
     8461 tgctgatgga ccaacctcac tgccatagag ccctcgccca ccgcggaggc gcaccgcttg
     8521 atcgagccgc tgcgcacgtc acccgcggcg aatactcccg gcatgctagt ctccaggaga
     8581 aacggctccc gcgaacgttc ttgccagtcc tcctcatgga gcgcagaatc gttcagctcc
     8641 ctgccggtca ggacgaagcc gcgctcgtcc agttcgacgg caccttggag ccagccggtg
     8701 ttggcctccg cgccgatgaa gacaaagagc gcccgcgccc caagggtgcg gcgtgccccc
     8761 gagcggttgt cctcgatcac gacccccttc aagtgatcct ctcccgtgag ctctctcacc
     8821 ccggtgctga ccagcagctc gatgttctcg gcgttcgtaa tccggtcgac gaggtacctc
     8881 gacatgttct tgcctagatc ctctcccctt atgagcaggt aaaccctcct tgtgcgtccc
     8941 gccaggaaca gcgctgcctg gcccgccgag ttgcccccgc cgacgatcgc gacctcctcg
     9001 ccctcgcacc tctgtgcctc ggcctccgta gctgcgtagt ggacgctcac gccctcgaaa
     9061 cgctccaggc ggggtacctc tagcctgcgg tagcgggccc ctgttgcgac tatcacgctg
     9121 cgtgccgcaa cctcaccgcc ttcggagagc tgtatatagt agtggccgtt ttccctcctc
     9181 agaccgagcg cctcccgagg cactgcggtg cgggcgccga acttgtcggc ctgcaccagg
     9241 acgcggctgg ccagctcgaa gccggagagg cctgcgggaa agccaggata gttctctatc
     9301 cgagatgagg tgcccgcctg gccgcccaag gctatcgact ccagggcgag ggtagagagg
     9361 ccctccgagg ccccgtagac tgccgcgccg aggcccgccg gccctgtgcc gaccacgacg
     9421 aggtcgtagg ttctatccag gggggcatct acttcgaggc ctgtggtgcg ggcaaactcg
     9481 gcattcgtcg ggttcttcag cacgtcctct ccctgccaga tggtgaccgg cgtctccgag
     9541 ggctttgcac cgaacctctc gagcaaggct tcggccccgg ggttctccac gagctcgatc
     9601 cagacgtggg gcacccggtt gcgggccgca acctccctga ggcgcgtcgc gtcgctcgag
     9661 tggcgcgagc ctattatcct caggcccagg ccggtcctga tcatgtaggt gcgcctggcc
     9721 aggaaggctt tcaggatgac atccgagaga ttgggctcct cggtgacgat ctccttcagc
     9781 cgctcgcggg ttatggccag cacctccccg ccttcgcgca ccacggccgt gaggtacatc
     9841 gcctgcccgg tgagcatgtt cagctcaccc aagaaacggc cagccaggaa gacacctatc
     9901 gtccgtgccc cgtacgcgaa gtcgtcgacc acctctacct ggccctcgag gatgactatg
     9961 aagtcgttct gggtttcacc cgccctgaac aggacatccc cgacctccgt cttcctcttc
    10021 tgcccgtagc gcctgagaat ctcgatctgg tcttccctca gcctcggggc cctcgtacta
    10081 cggatctcat ccattcgatc cttcatgttg cgacctcttc gatagccctt actactattg
    10141 caagcataat gcctcctacg gcaggccggc gcatcctcac aattggccat gtttcccttg
    10201 cttgggcgtc gccattggct tgcaaaacct ccgaaaacct tgaagagccg gctgcttgaa
    10261 gcaggtctct cgtgtaccat cctttgtcag gagcaccagg agatacgcgt gaagataacc
    10321 ggcgtcgaga catatccggt gtggggcgga gagcggaatt acctgttcgt ggtcgtggat
    10381 accgacgaag acctctacgg cgtgggcgaa gcggggctta ccggccgcga gctggcggtg
    10441 acgggaacga tcgagcattt cgagcccctg ctcctgggcg agaacccttt tcgcaccgag
    10501 cacctctggc aactgctctt tcggggaggc ttcttccccg ccaggcacgc cgtttcggcg
    10561 gccatctcgg ccgtggacat tgcgctatgg gatatcaagg gcaaagcctt gggcgttccc
    10621 ctctacgagc tcctcggtgg gcgagcccgc gaccgggtgg tctgctaccc gcacaacgcc
    10681 gaagccggca cggacacggc cgcgctggtc gagtcctgct tgcagaccac ggacgagggc
    10741 tggcgcttcg tgcgctgggg tctgccccaa gagggcgagg tcttcgagcc tcggcgttct
    10801 acgttagccg ccgtcgaaca gctggcggcg gtgcgcgatg ctgtgggcga ggagatcgag
    10861 atgatactcg acgttcacac ccgcctcgag atgcccgact cccttcggct ctgccgcgag
    10921 gcagagccct tgcgccccta cttcatcgaa gaccccttgc gttcggagga ccccaggtcc
    10981 tacgagttgt tgcgactgcg caccgccgtg cccttggcag ccggggagca attcggctcc
    11041 aagtgggagt tcagggagct cgtggagagg gagtggatag actacgcccg cgttgatccg
    11101 tgcatcgccg ggggcatcac cgaggcgaag aagatcgcgg cgatgtgcga gacgcaccac
    11161 gtaaagctcg ccgtccacaa cccgctcggg ccggtctcga cggccacctg cctgcacctg
    11221 aacctctcgt gccctaactt cggtgtccaa gagcaaccct acaggccagg aacgttcctg
    11281 accgacgtcg tccctgtgca gcccaagtgg gaggatggtc acctgctgcc gcccacgcga
    11341 ccaggactgg gcgtggagtt cgatcgggag gcggcgaagg gtcgtccctt ccggatggcg
    11401 gagcttccgc acctgcgccg cccggacgtt tcggttacga attggtgagc gagccccatg
    11461 ttgcgaagcc cacctcggac ggttttgtac ttgagaagct agacgacagt ttgcgagacg
    11521 tccaaagcac cgatcaaagc gtagagctgg ccatccgccc gggcaacatg tctcggccaa
    11581 ccaacaagta ttccctacct tattgcaggt tctataccat gcgttctagc acgtcgatga
    11641 tcgtcgcgta ttcttgttga gtgatatctt tgggagcatg ccccggacct tctgttacgg
    11701 caagagagac gttagtcgct agcctgctcc ttcttgagct gctcgaccaa tacctccctc
    11761 tccctacgcc ggtcgcgacg ggtaagagct tcccgcctac ggtacctatg cgctccctgc
    11821 ttgagtctgc gggtttcttc ttcgagaccc cggttcccgt cctgcccttc ggtgcaccct
    11881 ctcgttccct ccttgttgcg ttcgtagagg atcctgcttg caacatcgca tgtcggtacg
    11941 tatgccctac ccactgccct gagatacaaa acgtgcggag taagagcgtg gaatgagcat
    12001 ctttcctaaa tgtagccctc gcgcccagga acttctaagg tgggcttctt cggattgtcc
    12061 cggctcgtcc gccatggtct ccttgtgtcg atcccttgcg tagaattcct cctaccctca
    12121 ccggctgtcg gcgagcgggt ggacggatga gaaggaaagg atggagcgca tgagaaagca
    12181 ggagttatcg ggtggcttag gctcgcggga ggttagcacg ccgcgcttga cgactcctct
    12241 gctggagagc ggaccggagg agggcgtacc cgtagtgttc gtccacggct gcggcacact
    12301 ccgcacatag aaaagcctga tgcgtttcga cgagtcctca tcgagtttct cccttgagac
    12361 cgctccacgc cgaggctcgt taacccccgg tacctcaatg cgccaaggcc tgactacatc
    12421 ctacccgctg acacagggag ttctcacgca ccagctctac gggcccacac ctggaaagtg
    12481 ggcgctatgc tcatcaacgt ccggtcgtcg tcggcgagcc tgcccagttc ggcgacgaca
    12541 ttatccacct cgcggccgga caccaacccg gcgcccacca ccgcctccct tatgtgctcc
    12601 ggttcaggag gcgcagtcgt tccgctcgac cgccccgcag ggcgtaaccc tcggaccgcg
    12661 acgaatgtcc catccccatg cccttcagcc tgctgccatc gtatacttta ccccctcggc
    12721 atgatgccac tcggtccggt ccggtgctcg agtcgagcac cggacgcagc gagagaatgc
    12781 tcgagggcct gctaggaggt ataggtggga gcaggttcta ccgccttctg tgggtggaag
    12841 ccagttggaa caacgccgaa actttgtgtg gtactggccg aggggcccgg tgagtgacag
    12901 atcgccgttg ggtttcataa ctatcgtacg tatgcttcgt aaagcgtacg taggagcagg
    12961 cgttgaggtt tcgcgagaag agcagcgaag agtacacgcg tggcagatca acctggcacg
    13021 ctcttactgg ctcggaggac gaactgagac cgaaggttga gcacgtgggg gtcgagcgtg
    13081 cgagcataac ctctcgagca agatggtcgg actcgccata tgtggaatcg atcatgcatg
    13141 gtagaacctt gag
//
LOCUS       DASTKM010000022         6568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_531674, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000022 DASTKM010000000
VERSION     DASTKM010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531674"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="VFQ10_00760"
     CDS             <1..1259
                     /locus_tag="VFQ10_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET9963896.1"
                     /translation="REWWGENQRVLTDPGVAGIWCDMNEPALFIPHGATMPDDVVHPG
                     GRTGEARWHAQIHNTYGSLMARAARGGLLALRPDERPFVITRAGYAGLQRHAMHWTGD
                     NSSWWEHLWMSMPQLLNLALSGVAWAGVDVGGFGGDTNGELLARWTEFGVFQPYCRNH
                     TTIGTRRQEPWAFGEPYESVCREMIKLRQRLHPYLYSLFEECHRTGAPILRPLLFEYP
                     ADETTYTADDEFLLGDALLVAPITRPGIEHRHVYLPQGTWFHYYSGERFTGPTHILAH
                     APLGEPALYVRGNSPIPMGPDAAHTGERPNDPLTLLVYPDEGKGESTLYEDEGNGFGY
                     EEGEYARRRISCESSAGRITIRLGERQGSFVPERREVRLVLRGIPTAQGITVNDEERA
                     PDRVEGGALTVELGEEATPTTVEVIL"
     gene            1287..2177
                     /locus_tag="VFQ10_00765"
     CDS             1287..2177
                     /locus_tag="VFQ10_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9963897.1"
                     /translation="MLRQIFVPLITLLVGYFGVGFLVVLWMTSPSRKSPEATPASVGL
                     GYRGVEFPSTDGVRLSAWWVPVEGSSLAAVLVPGWGGYKFDEHLLQTLPVYHDAGYSV
                     LLLDLRAQGESDGTRRTLGYREVRDVRGALAWLQQQGYRLDQIVLHGWSMGGATALRA
                     APGTGVAAVVEEAAYADLPILLKGEIPEFVRFGRPLRPAILLAGRLFPDFDPWDVVPK
                     KDAAKLSDEGIPLFVIHSTEDGIVPYEQARIFAAAHPDARVWKLEGYGHVEAYQHPEY
                     AQRVRAFLLASRRRRKGVSV"
     gene            complement(2220..2873)
                     /locus_tag="VFQ10_00770"
     CDS             complement(2220..2873)
                     /locus_tag="VFQ10_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9963898.1"
                     /translation="MYRALLFDLDGTLAETDSLHLPTWVDALQPYGVEVDEEFYRDRI
                     SGRSTAQIVRELLPDVTDEEGRSIGDAKEASFRERASQLEPLPGLVDFVERARKRGMR
                     IALVTNAPEENVEAILPALKLRDIFAVVVLADEVEAVKPDPAPYKAALEKIGAPAKEA
                     LAFEDSVSGISSSVAAGISTVGIASSQEPQKLFAAGAFMTAQDFTDSQLQALIETQA"
     gene            complement(2878..3216)
                     /locus_tag="VFQ10_00775"
     CDS             complement(2878..3216)
                     /locus_tag="VFQ10_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963899.1"
                     /translation="MSSFYTILTDWLAWERRGPANRVLFVLSLSLAAFVTLTFAGASL
                     DPDPGNFVRYFAFYWVWGLCAIGVLLGMAEYKTSPLRKGRERGHLFILLYIGALLIVT
                     RALNMVLYAG"
     gene            3300..4424
                     /locus_tag="VFQ10_00780"
     CDS             3300..4424
                     /locus_tag="VFQ10_00780"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9963900.1"
                     /translation="MRRLLALSCAIVLVDTIFYAALTPLVPLFEEEFGLSKSAVGILS
                     GAYGAGVLVGSVPGGYLASRSGVRTTALVGLILMCVTSVMFALADTTWLLVAVRFAGG
                     FGSALSWVAAFTWLVDRAPEERRAEMIGVMISAAVVGALLGPVLGSVAATVGLLPAFA
                     SVAVVGGVIAVWAALEQAPISSGDISLLPALRTLREPLLLTSLWFIGLSPLLFSALAV
                     LVPLDLNRLGWGAATVGTVFFVSAIFEAFVHPLLGRWSDRSGYRPPVFAGLLTSIGIL
                     LLLTWVGNPWLIGLLVVLAAGAFNATLVPGTALFSRGTEKAGMERAVAFAITNFAWAS
                     GYAVGAPVGGFLADLRGDALSYLTLTGVCLLTIVLLRRTA"
     gene            complement(4513..4605)
                     /locus_tag="VFQ10_00785"
     CDS             complement(4513..4605)
                     /locus_tag="VFQ10_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HET9963901.1"
                     /translation="MGKQFWINILLTLLGYIPGIVHAVWVIAKY"
     gene            complement(4614..4997)
                     /locus_tag="VFQ10_00790"
     CDS             complement(4614..4997)
                     /locus_tag="VFQ10_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9963902.1"
                     /translation="MRIVVTSVFVEDQEKALGFYTEVLGFQKKTDVPLGEYRWLTVGS
                     PEEPNGVELLLEPSEHPAAKPFKQALVEDGIPFTSFGVEDVKAEHARLEGLGVKFTQP
                     PTTFGDVTTAVFDDTCGNLIQIAQT"
BASE COUNT         1223 a         2019 c         2055 g         1271 t
ORIGIN      
        1 cgcgcgagtg gtggggggag aaccagaggg ttcttaccga ccccggggtg gccgggatct
       61 ggtgcgacat gaacgagccg gcgctcttca taccccacgg ggccacgatg ccagacgatg
      121 tggtccatcc tggcggaagg accggtgagg ccaggtggca cgcccagatc cacaacacct
      181 acggctccct gatggcacgc gccgccagag ggggccttct ggccctgagg cccgacgaac
      241 ggccgttcgt gatcacccgc gctggctatg ctggcctcca gcgccacgcc atgcactgga
      301 ccggtgacaa ctccagctgg tgggagcacc tgtggatgag catgccacaa ctcctgaacc
      361 tcgccctctc cggcgtcgcc tgggccggtg tcgacgtggg aggattcggc ggcgacacga
      421 acggagagct cctggcccgc tggacggagt tcggagtctt ccaaccttac tgccgcaacc
      481 acaccacgat aggcacccgc cgccaggagc cgtgggcctt cggcgagccc tacgagtcgg
      541 tgtgcaggga gatgatcaaa cttcgccagc gactccatcc ctacctctac tcgctcttcg
      601 aagagtgtca caggacgggc gcgccgatcc tgcgcccgct gctgttcgag taccccgccg
      661 acgagactac ctacacagcc gacgacgagt tcctgctcgg cgacgccctc ctcgttgccc
      721 ccataacccg ccccggtatc gagcaccgcc acgtgtattt gccccagggc acctggttcc
      781 actactacag cggcgagcgc tttaccgggc cgacccacat cctggctcac gcacccctgg
      841 gcgaacctgc cctctatgta agaggaaatt ctcccattcc gatgggccct gatgctgccc
      901 acacgggcga gaggccgaac gatccgctga cgctgctagt ctacccggac gagggcaagg
      961 gagaatccac actctacgag gatgagggga acggcttcgg ttacgaagaa ggtgagtacg
     1021 ccaggcgcag gatctcctgc gagtcttcgg ccggccggat taccatccgc ctcggtgaac
     1081 gccagggttc gttcgtcccc gagcgtaggg aggtacgttt ggtgctacgc ggcattccaa
     1141 cggcgcaggg cataaccgtc aacgatgagg agcgcgcgcc ggaccgagta gagggtggcg
     1201 ccctcaccgt cgaactcggc gaagaggcca ccccgacgac ggtggaggtg atcctctagc
     1261 cgcccggcgc caggaggatg cgaacgatgc tccgccagat tttcgtcccc ctcatcaccc
     1321 tgttggtggg gtacttcggc gtgggatttc tagtggttct gtggatgact tccccgagcc
     1381 gcaaatcccc ggaggcgacg cctgcgtccg tcgggctcgg atacaggggg gtcgagttcc
     1441 cgagcacaga tggggtccgt ctgagcgcct ggtgggtgcc tgtggagggt tcgtccctgg
     1501 cagcggtgct cgtccccggc tggggcggat acaagttcga cgagcacctg ctacagaccc
     1561 ttcccgtcta ccatgacgcc ggctacagcg tgctgctgct ggatctacgg gcacagggcg
     1621 agtcggacgg tacgcgccgc accctcggct acagggaagt tcgcgacgtg cgcggcgcgc
     1681 tcgcctggtt acagcagcaa ggttacaggc tggaccagat cgtgcttcac ggctggtcga
     1741 tgggtggcgc aacggccttg cgcgcggctc cggggacggg tgtagccgca gtcgtcgagg
     1801 aggctgcata cgcggatctc ccaatccttc tgaaggggga gattcccgag ttcgtacgct
     1861 tcgggaggcc acttcggccg gcgatcctgc tcgcggggag gctcttcccc gacttcgacc
     1921 cctgggacgt ggtaccgaaa aaggatgccg cgaagctctc ggatgagggt ataccgctct
     1981 tcgtcatcca ctccacagaa gacggcatag tcccttacga acaggccagg atcttcgccg
     2041 ctgcccaccc cgatgcgcgc gtctggaagc tggagggtta cgggcatgtt gaagcgtacc
     2101 agcatcccga gtacgcgcag agggtgcggg cgttcctgct tgcatcccgg cgaaggcgta
     2161 agggcgtgtc cgtatgacgg cgtagggcgg ttcgcgaacc acccttgcac gcgacaccct
     2221 caggcctgcg tttcgatcag ggcctggagc tgcgagtcgg taaagtcctg tgccgtcatg
     2281 aaggctcctg ccgcgaagag cttctgtggc tcctggctcg aggcgatacc tacggtcgag
     2341 atgcccgcgg cgacggagga ggagatgccc gagacggagt cctcgaaggc cagggcttct
     2401 ttggcagggg cgccgatctt ctccagggcg gccttgtagg gggctggatc cggctttaca
     2461 gcctcgacct cgtcggcgag tacgaccacg gcgaagatgt ctcgaagctt cagggcgggc
     2521 agtatcgcct ccacgttctc ctcaggggcg ttggtgacta gggcgatccg catccctcgc
     2581 ttcctggctc gctctacaaa gtccacgaga cctggcaggg gttccagctg ggaggcgcgc
     2641 tcgcggaagc tcgcctcctt ggcgtcacct atggaccttc cttcctcgtc ggtgacatcg
     2701 gggagcagtt cccgcacgat ctgggctgta ctacggccac tgatcctgtc gcgatagaac
     2761 tcctcgtcta cctcaacacc gtatggttgg agcgcatcca cccacgttgg caggtgcagg
     2821 gagtcggtct ccgcgagcgt gccgtcgagg tcgaagagca gggcccggta caaaccgcta
     2881 accggcgtag aggaccatgt tgagagcgcg ggtcacgatg agcaacgcac cgatgtacag
     2941 aaggatgaaa aggtgcccgc gctcgcggcc cttgcggagc gggctcgtct tgtactcggc
     3001 catcccgaga agcacaccga tggcacagag accccacacc cagtagaacg cgaagtagcg
     3061 cacgaagttg ccggggtcag ggtcgagcga ggccccagcg aaggttagcg tcacgaaggc
     3121 ggccagagag agcgacagga cgaacagcac cctgttcgcc ggtcccctgc gctcccacgc
     3181 gagccagtcg gtcaagatgg tatagaaact gctcacaccg gtcatattag cagccacaac
     3241 gcggggcgca gtgaaaggta tcggctcacg cgccgtacgc agacattaga ataaccgtcg
     3301 tgcgccgact cctcgccctc agctgcgcga tagtgttggt ggatacaatc ttctacgctg
     3361 cgcttacgcc gctcgtccct ctcttcgagg aggagttcgg cctctccaaa tccgcggtcg
     3421 gcatcttgag cggggcctac ggggcaggcg tcctggtcgg tagcgtgcct ggtggatacc
     3481 tggcttcgcg gtcaggagtg aggaccacgg ccctcgtcgg attgatcctt atgtgcgtta
     3541 ccagcgtgat gttcgcgctc gcggacacga cgtggctact cgttgcggtg cggttcgcag
     3601 gcgggttcgg tagcgcgctc tcctgggtcg cggcgttcac ctggctcgtg gaccgggctc
     3661 cggaggagcg gcgggccgag atgatcgggg tgatgatcag cgcggccgtg gtcggggcgc
     3721 tcctcggacc cgtgcttgga agcgtcgccg caaccgtcgg cctcttgccg gccttcgctt
     3781 ccgtcgctgt cgtgggcggg gtcatcgccg tctgggccgc cctggagcag gcaccgatct
     3841 cctccggtga tatctccctg ttgccggcgc taagaacgct acgtgagccc ttactactta
     3901 ccagcctctg gttcataggg ctctcccccc tgcttttcag cgcgctggcc gtcctggtac
     3961 cgctggatct gaacaggctc ggatggggtg ctgcgacggt ggggacggtc ttctttgtga
     4021 gcgccatctt cgaggcattc gtccatcctc tgctcggccg ctggtccgac cgctctggct
     4081 accgcccgcc cgtcttcgcg gggttgctaa cctcgatcgg catcctgctc ctcctgacgt
     4141 gggttgggaa cccgtggttg atcgggcttc tcgtcgtgct agcggcaggg gccttcaacg
     4201 caacgcttgt ccctgggacg gcactcttct cccgcggaac cgaaaaggcc gggatggaaa
     4261 gggccgtggc cttcgcgatc acgaacttcg cctgggcctc cggttacgct gtcggggccc
     4321 ccgtgggagg cttccttgcg gacctcagag gcgacgccct ctcctacctc acgcttacgg
     4381 gggtctgcct gctgacgatc gtcctgctcc gccgtacggc ctgaagcgac agcactgcag
     4441 catttcggcc tgttgttttc gctcgattac gaacgtgcga ttcgcggtag gtcgatgcct
     4501 ctgcgaatca ctttagtatt tcgcgatgac ccacacagca tggactatgc cggggatgta
     4561 gccgaggagg gtaagtagga tgttgatcca gaactgctta cccaaagcgc ccactacgtc
     4621 tgggcaatct gaatcaggtt tccgcatgtg tcatcaaaca ccgccgtcgt gacatccccg
     4681 aacgtggtcg gaggctgtgt gaatttgacg ccaagacctt cgaggcgggc gtgttcggcc
     4741 ttgacatctt caacgccgaa actcgtgaac ggaatgccgt cctcgacaag agcctgtttg
     4801 aacggtttgg cggcggggtg ttcgctcggt tccagtaata gttcgacccc gttcggttcc
     4861 tccggtgaac caaccgtcag ccaacgatat tcgcctaacg gtacgtccgt cttcttctgg
     4921 aaccccaaga cttcggtgta gaaacccaaa gccttctctt gatcttcgac gaacacgctg
     4981 gtcacgacaa ttcgcattcg acttctcctt tccgcttgtg cttgttcaac cactcgatga
     5041 gccgaggcag gtgtgccgac agtttgctgg aatgcaactt cgtgcggccc tcccaacgaa
     5101 cgtctatgag atcggcgttt tccagcaccg tcaggtgctt ggagatcgcc tgccttgtga
     5161 ggttcatacc gtggccttcc acaaggcgaa cgcaaatctc gaacaacggt tggtcgtctc
     5221 gttccgccaa ctcgtctaga attacacgtc tggttgaatc agccaaagcg tgaaacatcg
     5281 catcgaggtc cctgtcgtca ctgactgcca tgttccttca tattatgcaa ccatgtggtt
     5341 gcgtgtcaag tatgcatcca agtgcgaacg ccctggatgg gccaaagtga actctcatgg
     5401 cagcttcaga gcactgcaga tcttctgtca ggctcatagc acctttgcct aacagggttt
     5461 atgtcttagt ttggaagaga gaagggagcc agcggaccca ctcgccgact cccttaccgc
     5521 ctaccttcgg agagacagtg cagactccag aaggaggcaa tgtggagact ctattctacc
     5581 cggcctagag ctgttctcca gggtaaggag cagcggcgag agtgatggtc aggggggaag
     5641 gaccataaag aggggtgctc gccgctgtaa tgaaagaagg gtacggaaag atgccgtgtt
     5701 ctccatacac aatcgtgcat atccatccat tcctgttaca agacgactgg tggatcaggc
     5761 ggatcggggt ggctgaatgg gcttcgccac accctcacag ggacgggtat tcaggatcgt
     5821 gaatctgaac ttccgagaaa tcctattcga cgcactcggg tgaataaggg caaagcgaag
     5881 ggccggagcc gaacgggaaa gctccgaccc catgatagaa gccggtgcgc cgagcacgga
     5941 ggaagttgca atgctcccgg gaatgtgccc gccccaccgg ggatagtcat ggtctcacta
     6001 ccgacgaccc agcgcaacgc ttcaaggagg caaagcgatg gccgcgtcga gaatagaagg
     6061 taaagaatga cgcggccaac agtagacgca ttataaggcc ggaacccgaa agccccaccc
     6121 cgatggacta aggccccacc cgagtggggt cgcttgctac cgggaacttc ggagaattct
     6181 tattccacgc actccggtga ataagggtaa ggagaagggc cggaggagat aagaagaggg
     6241 gatggagatt ccatcccctc ccctcaatag ttaattagcc cagcttaacg cgtagccgga
     6301 cagtactcct ggatttcagc gtttggatct ggctccgtct tgaacgccgg ggagtcgggt
     6361 ccctgggccg ttgatactga tacgaccggc tgcgctaccg ttgccttgct ctggtccgga
     6421 cctcccgggg tgaggccagg cgggtcctga ctcgtcggca catctgaggc cgaatcatta
     6481 gcacactcgg agtttgccaa ggggtgaacg gtagccaagg caggccctgc gaccgccatc
     6541 attgccgcca tgaccaacgc caccgtaa
//
LOCUS       DASTKM010000023         8079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1060341, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000023 DASTKM010000000
VERSION     DASTKM010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060341"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFQ10_00795"
     CDS             complement(<1..472)
                     /locus_tag="VFQ10_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9963903.1"
                     /translation="MATNWDVRFFQSTRGRIVTLLRRSKSTVEDLARALDLTDNGVRA
                     HLGVLERDGIVRQRGSLRRSSGGGKPAYVYELTSEGEELFPKAYEPTLGRLLDVLSER
                     LGPEESEALLRSVGRRLAEEHSTRADGTHARLETAVEVLNELGGLAELEERDGAL"
     gene            879..1712
                     /locus_tag="VFQ10_00800"
     CDS             879..1712
                     /locus_tag="VFQ10_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9963904.1"
                     /translation="MRYGIVMSNLDDYADPRLVIRLARAAEAAGWEAFFVWDHLGFAW
                     GSPSSDPWVSLSALAVSTTHLKLGTAITPLARRRPQVVANTLGSLDLLSGGRMIFGTG
                     LGGVPEEFTAFGEAGAAKERARMLDEGLAVLDRLWSGEMVTHHGQHYDVENVALAPLP
                     FQRPRIPIWIGGESPPALRRAARWDGWLAPATSPDGTPIMDKDPGRIAEMVAEIRRHR
                     TTDTPFAVAVDGYSDPDDPTLPHAYQVAGATWWLESIHGKRGSLDEMMARVEAGPPEP
                     R"
     gene            1771..2715
                     /locus_tag="VFQ10_00805"
     CDS             1771..2715
                     /locus_tag="VFQ10_00805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HET9963905.1"
                     /translation="MRAIAEETFDGPIALMDLPTPEIGAGEVLIRVRAAGVNPFDWKV
                     ADGALEDQMKHRFPLIPGFDAAGVIERVGAGVTELAEGDEVYGYLFKPVIGDGTYAEY
                     VGAPAPIVARKPVTVGFAEASALPTPGLTAMDLVDAVDTREGETILIVGATGGVGSYA
                     TQLAARRGAHVIATARRTNEALVRKLGAAETIDHTTEDIVDAVRMAHPGGIDAVIDVA
                     SDREALGRISTLVNEGGRLASSVYAADVEGLARRGIGATNVSMQPESRRLDKLSRMVD
                     SGELSVRLDRTFPLENAPQALEDRRTGHIRGKIVLLIH"
     gene            complement(2866..3774)
                     /locus_tag="VFQ10_00810"
     CDS             complement(2866..3774)
                     /locus_tag="VFQ10_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9963906.1"
                     /translation="MRVLVTGARGKVGRATVAALQKAGHDVRATDLTPPVFERPEEGE
                     ADYVQADLSDAGDAFAVVRGAEAVVHTAAIPEPTSNPPHVVFQNNLMATFNALEAAIR
                     FRVSRFVNTSSETVPGFFFPERPFLPDYVPVDEEHPIRPQDPYATAKYFGELLMDSAI
                     RRSDIRCISIRPSWVQHEGNYERNLGPMVRDRSELSAGIWSYIDVYDLADALVLATES
                     DLPGHEVFYIASPDNVGGRPFEAAVREHYGDQVEVRTPLPREDSSGISIEKARRMLGY
                     DPKRSWRGYLDDDGRLKPGIKGLFAD"
     gene            3862..4917
                     /locus_tag="VFQ10_00815"
     CDS             3862..4917
                     /locus_tag="VFQ10_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9963907.1"
                     /translation="MRIVVVGATGNIGTSVLRSLENEDSVESVLGLARRLPDLTMPKV
                     EWASADVVDDDLVPHFRNADAVVLLAWLIQPSRDLTKQWMVNVEGSTRVARAVKEAGV
                     PSLVYASSVGAYSAGPKDRRVDESWPTGGVPTSYYGRHKAEVERRLDRFEAEAPEVRV
                     VRMRPGLVFKKEAAEGIRRLFAGPFFPSVLANPALISVIPDIENLRSQVVHSFDVGEA
                     FRLAIVNKDARGAFNLAAEPVLDAGEIGRILNARPVPMSAQLARAGAGLTWALRLQPV
                     PPGWLDLSRNVPIMDTTRARTELGWTPQHSADEALLDLLEGLRTRSGFDTPPLSPKTG
                     GRFRIHEILTGVGGREP"
     gene            4914..6083
                     /locus_tag="VFQ10_00820"
     CDS             4914..6083
                     /locus_tag="VFQ10_00820"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HET9963908.1"
                     /translation="MKAVVWHGKEDVRVDNVPDPIIQESTDAIVRITSTAICGSDLHL
                     FAKLSPLMREGDIIGHEPMGIVEEVGADVEHIAPGDRVVIPFNISCGHCYFCDQELYS
                     QCETTRDTGKLAQTVNLLGRGRGASLFGYTHVYGAVPGGQAEYLRVPQAHFGPIKVPE
                     GPPDERFLYLSDVLPTSWQAVAYADIPEGGTAGIWGLGPIGQMCARIAMHLGAGRVIG
                     VDNVPERLAMASRHGVQTVDYSAVDSVRDVILNLTDGRGVDSAIDAVGMEAGGSLVGS
                     ILQSTKLQLDRATALRDSVSAVRRGGTLSITGVYAGPIQAFPLGDLFDMQIQLRMGQA
                     NVWQWVEDIVPLLNDDDPLDVEDLKTHEMPLEDAPLGYEMFQKKQGGAIKCVLKP"
     gene            complement(6119..7138)
                     /locus_tag="VFQ10_00825"
     CDS             complement(6119..7138)
                     /locus_tag="VFQ10_00825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9963909.1"
                     /translation="MKLDAGLGTEGEYLKGMDKTARAAEDLGFAGLWTSETKHDAFLP
                     LAIAANASHQIELGTSVAIAFSRSPMETAQTSWDLQDLSAGRFVLGLGTQVKAHITRR
                     FSMPWDRPAARLREYILALRAIWESFQTEVPLQFEGEFYRHTLMTPFFNPGPIEHPEI
                     PLYIAGVNTRLAELAGELCEGFHVHPFHSPEYVRRTVIPAIAEGARQANRDLDQVTLA
                     TSAFVITGENRESATEQRESVRAQISFYASTPTYRTVLEAHGWEEVGERLGTMAREKK
                     WPEMPALITDEMLAAFAIEAAPDEIGPALKERYDGLIDRVALYLPFVPGEHDEFWRTV
                     VDSAH"
     gene            complement(7148..>8079)
                     /locus_tag="VFQ10_00830"
     CDS             complement(7148..>8079)
                     /locus_tag="VFQ10_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HET9963910.1"
                     /translation="LGYEDVEIPEPGPGQARVKLAASGVNFIDVYQRTGLYPGDLPRT
                     LGLEGAGEVDAVGEGVDDVSPGDYVAFASTPGSYAEYVVAPAENLVPFNATLVEPRVA
                     AAAMLQGMTAHYLTHSTFPLEEGQTALVHAAAGGVGHLLVQLAKMRGATVIGTAGTEE
                     KAKLAREAGADEVILYTEQDFVEETNRITGGVGVHVVYDSVGKNTFDGSLDVLRRRGY
                     MVLFGASSGPVPPFDPQILNQKGGLFLTRPALVHYTATREELLWRAESLFSWIGQNNL
                     DVRIGGTYSLEDAEQAHRDLEGRKTTGKLLLIP"
BASE COUNT         1441 a         2508 c         2653 g         1477 t
ORIGIN      
        1 ctagcgcacc atcccgttcc tcgagctcgg cgaggccacc cagctcgttg agcacctcaa
       61 ccgccgtctc cagccgcgcg tgcgtcccat ccgcccgcgt ggaatgctct tcggctaggc
      121 gacgacccac cgatctcagg agggcctccg attcctcagg cccaagccgc tcggacagaa
      181 catccagcag ccggccaagt gtgggttcgt aagccttggg gaagagctct tcaccctccg
      241 atgtaagctc gtagacgtaa gcaggcttgc cgccgccact gctgcggcgc agcgaacctc
      301 gctggcgcac gatgccgtcg cgctcgagga cgccgagatg cgcgcggacg ccgttgtcgg
      361 tgagatccaa tgccctagcg aggtcctcca cagtgctctt ggaccgtctg agcagcgtga
      421 cgatccgccc tcgcgtgctt tggaagaacc tcacgtccca gttcgtagcc aatagtcact
      481 ccctccgctg gtcagcttca gtaaagcgca caaaccaaat ttttcaagta aatgaagttt
      541 atacttgaca tattatggta gagatgtata gactgcgtgg caggcagcga ctctcgagga
      601 ggtacagatg gaatccatgg cacccaacgc tgtagcgctg tgccgctgcg gtgggccgac
      661 gacggagtca ttctgcaacg gtactcacac taaggtcggg ttccgtgcag cgcagagagc
      721 ggttcgaaag gaagggggca ggtgtgatca tgtcagccac ccgacgggtt gcccaggcga
      781 gccttccgac tcgttgtgga accttcgaga cgtttgtcta cgacacaccc gagcacaaag
      841 aacacgtggc gttcacttga aaacaaggag cgtataggat gcgttacggc atcgtaatgt
      901 ccaacctcga tgattacgct gacccgcgcc tggtgatccg cctggctcga gcggccgagg
      961 cggcaggatg ggaggccttc ttcgtgtggg atcacctcgg tttcgcatgg ggttcgccct
     1021 cttccgaccc ttgggtcagc ttgtccgccc tcgcggtctc caccacccat ctcaagttgg
     1081 gaaccgccat caccccgctg gcacggcggc gcccgcaggt agtggccaac accctgggaa
     1141 gcctggacct ccttagcggt gggcgaatga ttttcggaac tgggctcggg ggggtgccgg
     1201 aggagtttac cgccttcggt gaggcgggcg ctgcgaagga gcgtgctcga atgctcgatg
     1261 aggggctcgc ggttctggac aggctctggt cgggcgagat ggtgacgcat cacgggcaac
     1321 actacgacgt cgagaatgtg gccctggcgc cgctcccgtt ccagcgtccg cgcatcccga
     1381 tctggatagg cggcgagagc cctcccgctc tgcgccgtgc cgcccgctgg gatggttggc
     1441 tcgcgcccgc cacaagccct gacggaaccc cgatcatgga caaggacccc gggcgcattg
     1501 ccgagatggt agccgagatc cggcggcacc gcacgacgga cacgcccttc gcggtagccg
     1561 tcgacgggta ctccgacccc gatgatccca cgttgccgca cgcctatcag gtcgccggcg
     1621 caacctggtg gttggaaagt atccacggca agcgtggctc gctcgacgag atgatggcgc
     1681 gggtggaagc gggccctccg gagccgaggt gagagttcga tgcgggccac aagtagatcc
     1741 tggtgtccta tcggtattga aaggagagag atgcgagcaa tagccgaaga gacgtttgat
     1801 gggccaatag ccctgatgga cctgccgact cctgagattg gggccggtga ggtcttgatc
     1861 cgtgttcggg ccgctggggt caaccccttc gactggaaag tcgccgacgg cgcgctcgag
     1921 gatcagatga aacaccgctt ccccctgatc ccaggcttcg acgctgcagg agtgatcgag
     1981 cgcgttggtg ctggtgtcac cgaactcgct gaaggcgacg aggtttacgg atacttgttc
     2041 aagccggtga tcggggacgg tacctacgcg gagtatgtcg gtgcaccagc acccatcgtc
     2101 gccaggaaac cggtgacggt cggcttcgcc gaggcgtcgg ccctacccac gccggggctc
     2161 accgccatgg acctcgtgga cgcggtggac accagggaag gcgagactat tttgatcgtg
     2221 ggggcaaccg gcggcgtcgg ctcctacgcg acccagctgg cggccagacg cggcgcccac
     2281 gtcatcgcca ccgcgcgccg aaccaacgag gcgctcgtgc gcaagctggg agcagcggag
     2341 acgatcgacc acaccacgga ggatatcgtc gacgccgtcc gaatggctca cccgggcggt
     2401 atagatgcgg tcatagatgt ggcaagcgac cgcgaagcgc tcggccgcat atccacgctc
     2461 gtaaacgagg gtgggaggct cgcgtcgtcg gtttacgctg ccgacgtgga ggggcttgcg
     2521 aggcgtggca tcggggcgac gaacgttagc atgcagcctg agtcccggcg gctggacaag
     2581 ctctcgcgga tggtcgactc cggggagctc tccgtgaggc tagatcgcac cttcccgctc
     2641 gagaatgcgc ctcaggcgct tgaagatagg aggaccgggc acatacgtgg caagatcgta
     2701 ttgctcatcc attgaggagc agtaatgctc acgcaggggt cacctccatt attggcaacg
     2761 ccataagcgt cgaagacgaa gagtacaagc tccgcaccat agtcgagacc gcggagcagg
     2821 tctggaggta gactaagacg ctcctcagag ggtctgtccg gtgcgctagt cggcgaacag
     2881 gccctttatg cccggtttca ggcgtccatc gtcgtcgagg tagccgcgcc aggagcgttt
     2941 gggatcgtag ccgagcatgc gcctggcttt ctcgatggag atgcccgagg aatcttcgcg
     3001 ggggaggggt gtccttacct cgacctggtc cccgtagtgc tcgcgcaccg cagcctcgaa
     3061 cggcctgccg cccacgttat ccggcgaggc gatgtagaag acctcgtgac ccggcaggtc
     3121 gctctctgtg gcgagcacga gggcgtcggc caggtcgtag acgtcgatgt agctccagat
     3181 tccggcgcta agctccgagc ggtcgcgtac catgggtccc aggttacgct cgtagttgcc
     3241 ctcgtgctgc acccaggaag gccggatcga gatgcacctg atgtccgagc gccggatggc
     3301 gctgtccatc aggagttcgc cgaaatactt cgctgtcgcg tatgggtcct ggggacggat
     3361 ggggtgttcc tcgtctacgg gcacgtaatc aggaagaaag ggccgctcgg ggaagaagaa
     3421 ccctggcacc gtctcgctgg aggtgttcac gaaacgggac actcggaacc ggatcgccgc
     3481 ctccagagca ttgaacgtcg ccatgaggtt gttctggaac acgacgtgcg gcggattgct
     3541 ggtcggctcg gggatcgccg ccgtgtgcac gacggcctcg gcgccccgca cgactgcgaa
     3601 ggcgtcgcca gcgtcagata ggtcggcctg gacatagtcc gcctcgccct cctcgggccg
     3661 ctcgaagacc ggcggcgtca ggtcagtagc ccttacgtcg tggcctgcct tctgtagtgc
     3721 ggccactgtg gcccttccga ccttcccccg cgcacctgtt accagtactc gcatcgatcc
     3781 tcccgctctc gctcccagcc gatggtacaa catcccgctt ctgggcgttt cacccatcgg
     3841 gtagcagggt aaaggccgtg catgagaatc gttgtagtag gggccacggg caatatcggg
     3901 accagtgtgc tccggtcgct cgaaaatgaa gacagtgtag aatccgtcct gggattggcg
     3961 cgccggctgc ctgatcttac gatgccgaag gtagaatggg cctcggcgga tgtggtcgat
     4021 gacgatcttg tccctcactt tagaaacgcc gacgctgtgg tactcctcgc ctggctcatc
     4081 caaccctccc gggatctcac aaagcagtgg atggtgaacg tcgaggggag cacgcgggtc
     4141 gccagggccg taaaggaggc tggtgttccc tcgctcgtct acgcctcgtc ggttggcgcg
     4201 tactcggccg gacccaagga taggcgcgtg gacgagagct ggccgaccgg tggcgtcccg
     4261 acctcgtact acggccgcca caaggcagag gtagagcgta ggctcgaccg cttcgaggcg
     4321 gaggcgcccg aagtgcgggt cgtaaggatg cgccccgggc tcgtcttcaa gaaagaggcg
     4381 gcggagggga taaggcgtct cttcgcaggg cccttcttcc ccagcgtgct cgccaacccg
     4441 gccctgatca gcgtcatccc cgatatagag aatctgcgct cccaggtggt ccactccttc
     4501 gacgtgggcg aggctttccg gctcgccatc gtgaataagg acgcccgcgg cgcgttcaac
     4561 ctcgcggccg agcccgtact cgatgcaggg gagatcggac ggatcctgaa cgcccgtccc
     4621 gtgccgatgt ccgcccaact cgcccgcgct ggcgcggggc tcacatgggc gctgcggttg
     4681 cagcccgtgc cgccggggtg gttggacctc tcgcgaaacg tccccataat ggatactacg
     4741 agggcccgta cggagctcgg ctggaccccg cagcacagtg cagacgaagc cttgctcgac
     4801 ctcctcgaag ggttgcggac ccgctcgggt ttcgacacgc cgccgctctc gccgaagact
     4861 ggcggacgtt tccgaataca cgagatactc accggagtag gaggaaggga gccgtgaagg
     4921 ccgtagtctg gcacggcaag gaagacgtca gggtggacaa cgtccccgat ccgatcatcc
     4981 aggagtccac cgacgccata gtaaggatca cctcgaccgc gatctgcggc tcggatctac
     5041 acctcttcgc caagctctcg ccgctgatgc gcgagggaga catcatcggg cacgagccga
     5101 tgggtatagt cgaggaggtc ggggccgatg tcgagcacat cgcgcccggt gaccgcgtcg
     5161 tgatcccctt caacatctct tgcgggcact gctacttctg cgaccaggaa ctctactccc
     5221 aatgcgaaac cacgcgggat acgggcaaac tcgcccagac cgtgaacctg ctgggacgtg
     5281 gcaggggggc gagcctcttc ggatacaccc acgtctacgg cgccgtccct ggcggacagg
     5341 cggagtacct gcgcgtgccc caggcgcact tcgggcctat caaagtaccc gaaggacccc
     5401 cggacgagcg gttcctgtac ctctccgacg tcttgccgac gagctggcag gctgttgcct
     5461 acgcggatat tcccgaaggc gggaccgcag ggatttgggg tctcggcccg atcggccaga
     5521 tgtgcgcccg catagcgatg cacctgggcg ccggccgtgt tataggcgtg gacaacgtgc
     5581 ccgagcgtct cgccatggcc tcgaggcacg gcgtccagac cgtagattac tccgcggtgg
     5641 acagcgtgag ggatgtgatc ctgaacctca ccgacggccg tggcgtcgac tcggccatag
     5701 acgccgtagg catggaggcc ggaggctcgc tcgtgggttc catcttgcag agcaccaagc
     5761 tgcagctcga tcgggccacc gccctgcgcg acagcgtgtc cgcagtgaga cgcggcggaa
     5821 cgctctcgat caccggcgtc tacgcggggc ctatccaggc cttcccactg ggcgacctct
     5881 tcgacatgca gatacaactc cgtatgggac aggccaacgt gtggcaatgg gttgaggata
     5941 tcgtgccgct cctgaacgac gatgaccctc tggacgtaga ggacctcaaa acccacgaga
     6001 tgccactcga agatgcgcca cttggctacg agatgttcca gaaaaagcag ggcggcgcca
     6061 tcaagtgcgt cctgaaaccg taaccgacag gcggaccacc agtttcgtgc gggctatctc
     6121 aatgcgctga gtccaccacc gtccgccaga actcgtcgtg ctcgccgggc acgaacggga
     6181 ggtagagcgc gacgcggtct atgaggccat cgtagcgttc tttgagtgct ggtccgatct
     6241 cgtccggggc ggcctcgatg gcgaacgccg cgagcatctc gtcggtgatg agggccggca
     6301 tctcgggcca cttcttctcg cgcgccatcg ttcccagcct ctcgcctacc tcctcccagc
     6361 catgtgcttc gaggaccgtg cggtaggtcg gcgtggaggc gtagaacgag atctgggccc
     6421 ttaccgactc cctctgttct gtggcacttt ctctattctc tccggtgata acgaaggcgc
     6481 tggtcgcgag cgtaacctgg tccagatcgc ggttagcttg cctcgcacct tctgctatgg
     6541 ccggtattac ggtcctcctc acgtactcgg gggagtggaa agggtggacg tggaagccct
     6601 cgcagagttc gccggccaat tccgcaagac gggtgttgac gcctgctatg tagagaggaa
     6661 tctcaggatg ctctatcggg cccgggttga agaacggcgt catgagggtg tgtctgtaga
     6721 actcgccttc gaactgcagg ggtacttcgg tctggaagga ctcccagatc gcgcgcaggg
     6781 ccaggatata ctcgcgcagc cgcgctgcgg gtctatccca gggcatcgag aagcggcgag
     6841 taatgtgggc tttgacctgt gtgccgagac caagcacgaa gcgtcctgcg gagaggtcct
     6901 ggaggtccca ggaagtctgc gccgtctcca tgggggatcg ggagaaggcg atggcgacgg
     6961 aggtgccaag ctctatctga tgcgacgcgt tggcggctat ggccaggggc aggaaggcgt
     7021 cgtgtttggt ctcgctggtc cacagtcctg cgaagccgag gtcttcggcc gcgcgggcgg
     7081 ttttgtccat ccctttcagg tactcgcctt cggtcccgag gccggcgtcg agcttcacgg
     7141 tatcgtccta agggatcaag agtagcttgc ccgtggtctt gcggccttcg aggtcgcggt
     7201 gggcctgctc tgcgtcttcc agggagtacg tgccgccgat gcggacgtcg aggttgttct
     7261 gtccgatcca ggagaagagg ctctcggcgc gccacaggag ctcctcgcgg gtcgcggtgt
     7321 agtggacgag ggctggccgg gtgaggaaga gtccgccctt ctggttgaga atctgggggt
     7381 cgaacggggg cacgggaccg ctggatgccc cgaagagcac catgtagccg cgccggcgca
     7441 agacgtcgag gctaccgtcg aaggtgttct ttcctaccga gtcgtagacg acgtggacgc
     7501 ctactccgcc tgtaatacgg tttgtctcct cgacgaagtc ctgctcggta tagaggatga
     7561 cctcgtcggc ccccgcctcc ctggcgagct tcgctttctc ctcggtgcct gcggtcccga
     7621 tcacggtcgc cccccgcatc ttcgcgagtt ggacgagcag atgcccgacg ccgcctgcgg
     7681 ccgcgtgcac gagcgccgtc tggccttcct ctagcgggaa ggtactgtgc gtcaggtagt
     7741 gtgctgtcat gccctggagc atggcggccg cggcgacccg cggctcgact agcgtggcgt
     7801 tgaaaggcac caggttctcg gcgggggcga ctacgtactc cgcgtaggat cccggcgtgc
     7861 tcgcgaacgc aacgtagtct cctggcgata cgtcatcgac gccttcaccc acagcgtcta
     7921 cctcgccggc gccctcgagc ccgagcgtac gtggcaggtc gccgggatag aggcccgtgc
     7981 gctggtagac gtctataaag ttgacgccgg aggcagcgag cttcacgcgc gcctggccgg
     8041 ggcccggttc ggggatctcc acgtcctcgt agccaagaa
//
LOCUS       DASTKM010000024        13293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1412414, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000024 DASTKM010000000
VERSION     DASTKM010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13293
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412414"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VFQ10_00835"
     CDS             complement(<1..1316)
                     /locus_tag="VFQ10_00835"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9963911.1"
                     /translation="MRLVILGGVAAGTKAASRARRLDPEMEITVYQEEPEPSISECGL
                     PYLLSGVVEERDDLVARTPEKFAEKGIEVLVRHRVERIDPDRKNLCVLNLNTGEAFED
                     TYDRLVIATGARAVLPPIPGAELEGVFKLRFLTDTDEIGRYIEEQSPSKATIVGGGYI
                     GLEVAENLCRLGMEVSLIEGEDRVALAYGPEVSENVEAELDANGVGVYTGEKVDEFTG
                     EARVRGVKFGDRELETDLVIVGVGIRPNVELAAEAGVEIGTTGAILVDPHMKTSVPDV
                     WAAGDCVETTNLVSGRSTWVPLGDTANQMGRVAGTNAATGEETLEFPGILGTGIFKVF
                     DLGVGKTGLSEKEAEDAGFEVVSAGLEAYDRAAYYPGAQKAFLKLIADRATGRLLGAE
                     SAGSGADKLTDICAAALWGSLSYPDLVNIDLAYAPPYGPALSPVIQA"
     gene            complement(1483..2112)
                     /locus_tag="VFQ10_00840"
     CDS             complement(1483..2112)
                     /locus_tag="VFQ10_00840"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET9963912.1"
                     /translation="MAYELPQLPYGYDALEPTIDEQTMHLHHEKHHNTYVTNLNAALE
                     KHPEADPGSLEELLANLDQVPEDIRTAVRNNAGGHSNHSMFWQIMGPSGQGGGEPSGD
                     LASAIDSSFGSFNSFKESFGAAAAPGALFGSGWCWLIANPDGSLSIETTPNQDTPLME
                     GKTPVIGLDVWEHAYYLKYQNRRPEYIGAWWEVVNWDEAERRYQAARNA"
     gene            2256..4427
                     /locus_tag="VFQ10_00845"
     CDS             2256..4427
                     /locus_tag="VFQ10_00845"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9963913.1"
                     /translation="METSSSRTRRGGDPERPASEEMRRLLDRAVAASSNGIVITDPKV
                     PDNPIVYVNPAFEEISGYTAEEVRGRNCRFLQGGYHEQPALDELREALKEERECRVVL
                     RNYRKDGTPFWNELYVSPVHDEDGLLTNFVGVQNDITERRRIEEVLRESEERSRATFE
                     HAAVGAAHVGIDGRWLRVNRRLSEIVGYEREELLERTFQDITHPDDLEGYLEQMRLML
                     EGELQTYTMEKRYLRKGGPEVWVNLTVSLVRDASGEPAYFIAVVEDISERKKTEQERD
                     LLLVREQLARAEAVAARRRLTLLAAAGPALAATLDYEQTLEDITRLIVPELADWCLLD
                     IAEDDGSVNQLAAAHAEEEKQQLLRKLREHRRFGEGDPGSTAEVLRTGQSMLISDLPD
                     TTFYERDLEGEHLEIVNRLEPRSLMCVPLLARGKTRGAITLVSSRLDRRYDREDLLLA
                     EDLAYRCALAADNARLYRDRSEIARVLQRSLLPPHLPEIPGVEVGAEYLPVGETNDVG
                     GDFYDLINTVEDGWLCAIGDVRGKGVEAASVTALARYTIRAVTLKDDRPSEVLAALNE
                     AMLRQLPEDRFCTAACVRLEPQDGSVGVGVDVSRAGHPPPLLVRSGATVEEVGCSGKV
                     LGVFPDAELRDTSLRLMPGEALVLYTDGVTEARSPDGEFFGEGRLRHLLSSCAGCDAA
                     TFARRIKGVVLDFQEGYPRDDLAVLVLRASDGAAPADRDLE"
     gene            4533..5939
                     /locus_tag="VFQ10_00850"
     CDS             4533..5939
                     /locus_tag="VFQ10_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET9963914.1"
                     /translation="MAQDADLPRSLADLSRELLARHLSPVEVVRALLGRIEVDETNSF
                     ITVTAERALEKASLAEREILAGRYMGPLHGVPVAIKDIIYTRGIRTTMGSALWAGHVP
                     DHSATVARKLEDAGSVMIGKTNTHEVAYGPTGDRSHFGPTLNPHDARRITGGSSGGSG
                     AAVAVNLCYGALGSDTGGSIRIPAALCGTVGMKPTFGRVSKSGVFPLSWSLDHVGPIT
                     RTAEDNAMMLNVLAGHDPEDHYSVDRPTEDFTRDLRRGLRGVSIGLPRSFYFDHVEEE
                     VGALVLAAVEVFVSLGAQVREVEIPNLVDTLHAHRLILGAEAYAVHEERLENEPETFG
                     EEISERLMDGARPRAYRYANARRRGVLAIDEFDHALQGVDVLLTPTLPIVATKIGQRE
                     VSIGDYEESVRSALTRYTGPTNLTGQPSLSIPCGTTASGLPVGLQLIGRHFDEATLYR
                     FGHAYEEAMVQDHQAAVR"
     gene            complement(6074..7513)
                     /locus_tag="VFQ10_00855"
     CDS             complement(6074..7513)
                     /locus_tag="VFQ10_00855"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9963915.1"
                     /translation="MSDSTMRVINPATGENIEEVPISSREEVDAAVERARDGFREWQG
                     FAGLDRAEVLHEISARMRENAHELAEVMTLEGGKPFIENRDEVTWTAACFDYYGEIAR
                     DSVGYLPAPIEPQQLALVVKEPVGTVACIVPWNYPLLLAAWKVAPALAAGNAVLLKPS
                     EETPLATRRLAELMHELPDGLLQILYGAGEVGSMLVRHPGVDMVAFTGSQETGRKIGI
                     AAAERLIPANLELGGKDPFIICDDVDVEVAAQGAVWAACLNAGQVCTSSERFYVMSKV
                     ADDFVEASREFVESLNIGDPMDRSTDIGPMINASGREKVERHVGEALDEGATLVAGGE
                     RYGERGFYYKPTVLTGVTPSMTVMRDETFGPVVPVVEVGSLDEAIEQANSVPYGLGAN
                     VYTQDFEKMLKCMRELRAGTVWINDPLTDNDAAPFGGQKGSGIGRELGREGLEAFQES
                     KHVHIDPRVEKKEWWYPYGNDDDPAQRTM"
     gene            complement(7572..9038)
                     /locus_tag="VFQ10_00860"
     CDS             complement(7572..9038)
                     /locus_tag="VFQ10_00860"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET9963916.1"
                     /translation="MSIATSEQSVVDSVNKQLFIGGEWRDASGGGTLNVEDPSTAEPL
                     CEIADGTAEDAMAALGAADEAQAEWSKHPPRERGEILRRAFEKIVERTDELALLMTLE
                     MGKALAESKAEIAYAAEFFRWFSEEAVRIEGRYAVPPAGAGRMLTMKQPVGPCIFITP
                     WNFPMAMGTRKIGPAIAAGCTIVMKPAQQTPLSMLALAGILEESGLPGGVLNVVTSSS
                     SGGTMGPIIADPRARKLSFTGSTEVGRKLVAQSAEQLLRVSMELGGNAPFLVFDDADV
                     DAAVEGAMVAKMRNIGEACTAANRFHVADGVAGEFAEKLANRMEAMKVGRGTEEGVEV
                     GPLIDGDQREKVAGLVEDALGKGARALIGAEKMEGSGYFYRPTVLDDIPEDATLLKEE
                     IFGPVAPVASFDSEEAAVAAANDTEYGLVAYVYTSDLKRAIRVCEGLETGMVGLNQGM
                     VSNASAPFGGIKNSGFGREGGKEGIEEYLETKYVAINL"
     gene            complement(9125..10558)
                     /locus_tag="VFQ10_00865"
     CDS             complement(9125..10558)
                     /locus_tag="VFQ10_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HET9963917.1"
                     /translation="MTETKTLKNFVGGEHVDAAGGRTYELVNPATGLTFAEAPVSEQA
                     DVDRAFEAADKAFETWRDTTPSERQLALLRLADAIEDRAEDLVRAESENTGKPFGLTM
                     EEEIPAGVDQIRFFAGAARVLEGKSAGEYLEGYTSFIRREPVGVIGQVTPWNYPMMMA
                     IWKFAPAIAAGNTIVIKPSDTTPVTTVMMAEIAAEFLPDGVFNVVCGDRDTGRMLVQH
                     PTPQMVAITGSVRAGMQVAEAAAHDLKRTHLELGGKAPVIIFDDADIEEAAEEIAGAG
                     YFNAGQDCTAATRVLAHPGIHDDFVAAITEQARNTTTGHPDDEVDYGPLNNANQLEHV
                     SGMVERLPDHAEVTVGGERFGDRGFFYSPTVVSGLRQEDEASQTEIFGPVVTVQTFSD
                     EDEAVRWANGVKYGLASSVFTKDHGRAMRVSRRLDFGCVWINTHIPLVAEMPHGGFKH
                     SGHGKDLSMYGLEDYTRIKHVMTNLEV"
     gene            complement(10555..11553)
                     /gene="speB"
                     /locus_tag="VFQ10_00870"
     CDS             complement(10555..11553)
                     /gene="speB"
                     /locus_tag="VFQ10_00870"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HET9963918.1"
                     /translation="MLDPLQRWASVGEKPDYAGLLTFAGVPYTQDAAHLEGVDVAIVG
                     APMDDLVSDRPGTRFGPRAIRAASSPSGPELETSVDAISELRVVDFGDAPVLPADPTR
                     SHAAIEATVAQVLAAGAIPVIIGGDHSISEPDVRACAAAYGPVGLVHFDTHTDTGTEV
                     FGVETSHGTIMRRLVEAGHVDPRRYAQVGLRGYWPGKEEFAWQTERGITSLFMHHIRD
                     LGIREVVRRAVDAVGDGPVFVSVDIDVLDPAFAPGTGTPEPGGMTTAELLWAVREVAM
                     QLEVIGADMVEVIPTGVGSADITALAADRIIREILGGIALRRRRQNTGQPDGKEER"
     gene            complement(11672..12490)
                     /locus_tag="VFQ10_00875"
     CDS             complement(11672..12490)
                     /locus_tag="VFQ10_00875"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9963919.1"
                     /translation="MGDAKGGPVYRGLGKIPDRLLFLFAGLVYLFLYAPIMVLIFFSF
                     NSTKNTQVWTGFSMQWYGDLIRDGSVLNAFKLSLIVGLTATAIATVIGTLTALALSRS
                     EFRGKTLADSAIYATTVMPEIVVGVSLLVFFVAATVELGIVTIIIAHVAFCISFVTIV
                     VRARLSGMDRSVEEAAQDLGATPVQTFLRVTLPLILPGVVAGALLAFTLSFDDFVITF
                     FVSGVGSSTLPLKIYSMIKFGVSPVINALSTVVIVVTMVLILGGSRFMSRKESA"
     gene            complement(12494..>13293)
                     /locus_tag="VFQ10_00880"
     CDS             complement(12494..>13293)
                     /locus_tag="VFQ10_00880"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9963920.1"
                     /translation="GLFFFVPLLLILAYSFGTSGVYGGITLGFNPSNYLKVFDPLYLE
                     IIVRTLVIALITTLLCLLIGYPLAYFITFKGGRHKHTLILLVMIPFWTSLLLRAYAWV
                     VILGGDNGIANRVLQFLNITDYPLTLIFTPQAVMLGMVYTYLPFMILPLYAALEKFDW
                     RLKEAAQDLGASRWHTFWRVTFPLSMPGVIAGSILVFIPSAGEFVIPNLLGGARTMMV
                     GNLIQQQFLSARDWAFGSALAIMLAVLLVGAIMFYVRRVGAEKLEEV"
BASE COUNT         2346 a         4260 c         4198 g         2489 t
ORIGIN      
        1 gcctgtatga cggggctgag ggcggggccg tacggcgggg cgtatgccag gtctatgttg
       61 acgaggtcgg ggtaggaaag gctgccccag agggccgcgg cgcagatgtc ggtgagcttg
      121 tcagcgccag agcccgccga ctccgcgccg agcagcctgc ccgtcgcccg gtcggctatg
      181 agcttgagga aggctttctg cgcgcccggg tagtaggccg cgcggtcgta agcctccagg
      241 ccagcgctca cgacctcgaa gccagcgtcc tcggcctcct tttcggagag gcccgtcttg
      301 ccaacgccaa ggtcgaagac cttgaagatg cctgttccga gtatgccggg gaactccagg
      361 gtctcttcgc ccgtggccgc atttgtgcct gcgacccggc ccatctggtt tgcggtatcg
      421 ccgaggggga cccatgtaga tctgccgctc acgaggttgg tcgtctccac gcagtctcca
      481 gcggcccaga cgtcggggac gctggtcttc atgtgagggt ccaccaggat ggctcccgtg
      541 gtccctatct caacccctgc ttctgcggcg agctcgacgt tcggccttat ccccacgccg
      601 acgatgacca ggtccgtctc aagctctctg tccccgaact tcacgcccct gacacgtgct
      661 tcgcctgtga actcatcgac cttctcgccc gtgtagacgc cgacgccgtt ggcgtcgagt
      721 tcggcttcca cgttctccga tacctcgggt ccgtaggcca gggcgaccct gtcctcgcct
      781 tcgatcaggg agacctccat cccgagccgg cacaggttct cagcgacctc gagcccgatg
      841 taacctcctc cgactatcgt cgccttgctg ggggactgct cctcgatgta tctacctatc
      901 tcatcggtat ccgtcaggaa gcggagcttg aagacgccct cgagctcagc gccggggatg
      961 ggggggagga cggcccgcgc gcctgtcgct atgacgagcc ggtcgtaagt gtcttcgaaa
     1021 gcctcgcccg tattcaggtt tagtacgcaa aggttcttcc tgtcggggtc tatcctttct
     1081 accctgtggc ggaccaggac ctcgatgcct ttctcggcaa acttctcggg cgtgcgtgcc
     1141 acgaggtcgt cccgctcttc gaccacgccg gagagtagat agggcaggcc gcactcgctt
     1201 atagagggct ccggttcctc ctggtagacc gttatctcca tctcggggtc aagcctgcgc
     1261 gcccttgagg cagccttggt cccagccgca acgccgccca agatcacaag ccgcataagt
     1321 gagaatcctc ccgctctgcc taccacccca gtctacaggc tttgcctcga gtgcgaagga
     1381 gtctcgatta caacgtctgg acacgcaacc ccgcgtaaga agaaaccccc ggaagcgcag
     1441 tccgggggtt tccactctcc ctacgttatc gagatgttac tgctaggcgt tccttgcggc
     1501 ctggtagcgc ctctcggcct cgtcccagtt tacgacctcc caccaggcac ctatgtactc
     1561 ggggcgcctg ttctggtact tcaggtagta ggcgtgctcc cacacgtcca accctatcac
     1621 cggcgtcttg ccttccatga gaggtgtgtc ctggttcggc gtggtctcta tggagagtga
     1681 cccatccggg ttggctatga gccagcacca cccactgccg aacaaggccc ctggtgctgc
     1741 ggcggccccg aagctctcct tgaaggaatt aaacgatccg aatgaggagt ctatagctga
     1801 ggctagatcc ccggaaggct ctcctcctcc ttgaccgctc gggcccatga tctgccagaa
     1861 catggagtgg tttgagtggc cgcctgcgtt gtttctcacc gccgttctta tatcttctgg
     1921 gacctgatcc aggttggcga ggagctcctc gagggaaccc gggtcggcct cgggatgctt
     1981 ctccagagcg gcattgagat tggtcacata ggtattgtgg tgtttttcgt ggtggaggtg
     2041 cattgtctgt tcgtctatag tgggctccaa ggcatcgtag ccgtagggca actgtggtag
     2101 ctcgtatgcc actgtcgttt ctcctctcgt ggtctggctt tcctttaccc accgtgttat
     2161 accccgaatt cgcgggctct taacgtcggg aggagattcc ttggtcgtcc tcgcacgctg
     2221 ggtgcggggt agcggaaggc tgtagaatac ggggattgga gacttcttct tcacgtaccc
     2281 ggcgcggtgg agacccggag cggcccgctt cggaggagat gcgacgcctg ctcgatcggg
     2341 cagtcgccgc gagctctaac ggcatcgtca taacggaccc gaaggtgcct gacaatccca
     2401 tcgtctacgt gaaccccgcc ttcgaggaga tctcgggcta caccgctgag gaggtcaggg
     2461 gaaggaactg ccgctttttg cagggcggct atcacgagca gcctgcgctc gatgagttgc
     2521 gggaggcgct caaagaagag cgggagtgca gggtggtctt gaggaactac cgcaaggacg
     2581 ggacgccgtt ctggaacgag ctgtacgtct cgcccgtcca cgacgaggat ggtctgctga
     2641 ccaacttcgt gggtgtccag aacgacatca cggaacggcg caggatcgaa gaggtcctgc
     2701 gtgagagcga agaacgttcc agggccacat tcgagcacgc cgccgtaggg gctgcgcacg
     2761 ttggtatcga cggccgttgg ctgcgagtaa accgacgtct gtccgagatc gtgggctacg
     2821 agcgggaaga actcctcgag aggacgttcc aagacattac ccatcccgat gacctcgaag
     2881 ggtatctcga acaaatgagg ttgatgctcg agggcgaact tcagacgtac acgatggaga
     2941 agcggtacct gaggaaaggt gggccggagg tctgggtaaa ccttaccgtt tcgctggtgc
     3001 gggatgcttc gggggagccg gcctatttta tcgcggtggt cgaggatatc agtgagcgca
     3061 agaagacgga gcaggagcgc gacctgctgc tcgtgaggga gcaactcgca cgggccgagg
     3121 cggtagcggc gaggcggcgc ctgacgttgc tcgcggcggc cggtccggcg cttgcggcta
     3181 cgctcgatta cgagcagacg ctggaggaca tcacacgcct catagtgccc gagctcgctg
     3241 actggtgcct gctcgacatc gcggaggacg acgggagcgt aaaccagctc gccgcggcgc
     3301 acgccgaaga agagaagcag caacttctga ggaagctcag ggagcacagg cggttcgggg
     3361 agggagaccc tgggagcacg gctgaggtgc tcaggacggg tcagtccatg ttgatctccg
     3421 atctacccga caccaccttc tacgagcgcg atctggaagg cgagcatctg gagatcgtga
     3481 accggctgga gcctcgctcc ttgatgtgcg ttcctctact ggcgaggggg aagaccaggg
     3541 gcgctataac actcgtctcg tcgagactgg atcgccgcta cgacagagag gatctcctgc
     3601 ttgccgagga tctggcctac cgctgcgcgt tggccgcgga caacgcccgc ctgtacaggg
     3661 atcgcagtga gatagcacgc gttttgcaga ggagcctgct tccgccgcac ctaccggaga
     3721 ttcccggcgt cgaggtcggc gccgagtacc tgcccgtcgg ggagaccaat gacgtgggag
     3781 gagacttcta cgacctcata aacacggtcg aggacgggtg gctctgcgcc attggagatg
     3841 tgcggggcaa gggggtcgag gcagcgtccg tgacggccct cgcccgatac accatccgcg
     3901 ccgtcaccct gaaagacgac cggccctctg aggtcctcgc cgccctcaat gaggcgatgc
     3961 tccgccaact tcccgaggac cggttctgca cagcagcctg cgtccgactc gagccccagg
     4021 acggctcggt gggcgtgggg gtggacgtct cgcgcgctgg ccatccgccg ccactcctgg
     4081 tccgctccgg cgccacggtg gaggaggtcg gctgttcggg aaaggtgcta ggcgtgttcc
     4141 ccgacgccga gctcagggat acgtcactgc gtctcatgcc tggcgaggcg ctcgtcctct
     4201 acaccgacgg cgtcaccgag gcgcgctccc ccgacggtga attcttcggc gaaggcaggt
     4261 tgcgccatct tctcagctcc tgcgccggct gcgacgctgc gacgttcgcc aggcggatca
     4321 agggcgtcgt cctggacttc caggagggct acccgcgcga cgacctcgcc gtcctggtcc
     4381 tacgcgccag tgacggagcg gcgcctgcgg acagggatct ggaataacct ccggaacgct
     4441 ctcctcgcgc gtggaatcac tcgtcgatgc tacggaaaca caccggagcg ctatgagata
     4501 ctgcacacgt ccaccctcga aggagcgaag gcttggccca agacgcagat ctgccacgct
     4561 cgctcgccga cctctccagg gagctcctgg cgagacacct ctctcctgta gaggttgtaa
     4621 gggcgttgct ggggcgtatc gaggtagacg agacgaactc cttcatcacc gttacggcgg
     4681 agcgggcgct ggagaaggcg tcgctggctg aaagagagat actggccgga cgctacatgg
     4741 ggcctttgca cggcgtaccc gtcgccataa aggacatcat ctacaccagg ggcatccgta
     4801 cgacgatggg ctccgctctt tgggccgggc atgtccctga ccacagcgcg acggtcgcgc
     4861 ggaagctgga agatgcggga tctgtcatga tcggcaagac caacacgcac gaggtagcct
     4921 acggtcccac aggagaccgt tcgcacttcg gtccgacgct gaaccctcac gatgcgcgaa
     4981 ggatcactgg cgggtccagc ggcggctcgg gggccgcggt cgcagtaaac ctttgctacg
     5041 gcgctctcgg ctctgacacg ggaggctcca tccgtatccc cgcagccctc tgcggcaccg
     5101 tcggcatgaa gcctaccttc gggcgggtga gcaagagcgg tgtctttcca ctctcctgga
     5161 gcctcgacca tgtcggtccc atcacccgca ccgctgagga caatgccatg atgctgaacg
     5221 tcctggccgg ccacgacccg gaagatcact actcggtaga ccggcccacc gaagacttta
     5281 cgcgggatct ccgacgagga ctccggggag tcagtatcgg tcttccgagg agcttctact
     5341 tcgatcacgt cgaggaggag gtcggggcgc tggtgcttgc ggccgtggag gtctttgtgt
     5401 cactcggcgc gcaggtacgg gaggtcgaga tccccaacct ggtggacacg ctccacgcgc
     5461 accggttgat cctcggcgct gaagcctacg ccgttcacga ggaacgcctc gaaaacgagc
     5521 cggagacgtt cggcgaagag atcagcgagc gcctgatgga cggcgcccgc ccgagagctt
     5581 accgttacgc gaacgcgcgt cggcgagggg tgctcgccat agatgagttc gaccacgccc
     5641 tccagggtgt cgacgtgctc ctcaccccga ccctcccgat cgtggcgacg aagatcgggc
     5701 aacgcgaggt cagtatcggg gattatgagg agagcgttcg ctctgcgcta acccgctata
     5761 cgggaccgac caatctgacg ggccaaccga gcctctctat accgtgtggg accaccgcct
     5821 ccgggcttcc ggtagggttg cagctcatcg gacgccactt cgatgaggcg accctctacc
     5881 gttttgggca cgcttacgaa gaggccatgg tccaggatca ccaggctgca gtccgctaac
     5941 cgggtccagg ccatccctcg ctccaagccc agaacccgcc tgcgctaggt atgcggccga
     6001 gatcagaccg tggtgccctg agccaacccc gagatgacgt cgtagcggcc gttacctctc
     6061 ctattcagcc acgctacatc gttctctggg ctggatcatc atcgttgccg tagggatacc
     6121 accactcctt cttctcgacc ctgggatcga tgtggacgtg ctttgactcc tgaaacgctt
     6181 cgagaccctc acggccgagc tcgcggccga tgccgctgcc cttctggccg ccgaagggtg
     6241 ctgcgtcgtt gtcggtgagg gggtcgttga tccagacggt cccggccctg agttcccgca
     6301 tgcacttgag catcttctcg aagtcttgcg tgtagacgtt cgccccgagc ccgtaaggca
     6361 cggagttggc ctgctctatc gcttcgtcca ggctcccgac ctccacgaca gggaccacag
     6421 gcccgaaagt ctcatcccgc atcacagtca tggacggcgt cacgccggtg aggactgtgg
     6481 gcttgtagta gaaaccccgc tcgccgtacc gctcgccgcc ggccacgaga gtagcgcctt
     6541 cgtcgagcgc ctccccgacg tggcgctcga ccttctcgcg gcccgaggcg ttgatcatgg
     6601 gtcctatatc cgtcgagcgg tccatcgggt cgccgatgtt caacgactcg acgaactccc
     6661 tggacgcctc gacaaaatcg tcggccacct tgctcatcac gtagaagcgc tctgaggagg
     6721 tgcagacctg accggcgttc aggcaggcgg cccacacggc gccttgggct gcgacctcca
     6781 cgtcgacatc gtcgcagatg ataaaggggt ccttcccacc gagctccaga ttcgcgggga
     6841 tgagacgctc ggcggcggct atgccgatct tgcgccccgt ctcctggctc ccggtgaacg
     6901 cgaccatgtc aacaccaggg tgccgaacga gcatggaacc aacctcgccg gccccgtaca
     6961 ggatctgcag cagcccgtcg ggcaattcat gcatcagctc agcgaggcgc cgcgtggcga
     7021 gcggcgtctc ctcggacggt ttcagaagca ccgcattccc ggcggcgagc gccggggcta
     7081 ccttccaggc cgcgagcagc agtggatagt tccagggcac gatgcaggct accgtcccga
     7141 cgggttcctt cacgacgaga gcgagttgct ggggttcgat aggcgcaggc aggtacccga
     7201 cggagtcacg ggcgatctca ccgtagtagt caaagcaagc agccgtccac gtaacctcat
     7261 cgcggttctc gataaagggc ttcccgccct ccagcgtcat gacctccgca agctcgtggg
     7321 cattctcccg catccgcgca gagatctcat gcaacacctc ggcccggtca agcccggcga
     7381 acccctgcca ctcgcgaaaa ccatcccgcg cccgttcgac ggcagcatcg acctcctcgc
     7441 gcgaagagat cgggacctcc tcgatgttct cccccgtagc agggttgatg acccgcatcg
     7501 tactgtccga catgatctcc tttcggtcgc cccgccaggg agtaggggcg cttcgcgaag
     7561 cgccccattc tctaaaggtt gatggccacg tacttcgtct ccaggtactc ctcgatgccc
     7621 tctttccctc cttcacggcc gaagccggag tttttgatgc cgccgaaggg ggctgaggcg
     7681 ttggagacca tgccctggtt gaggccaacc atgccggtct cgaggccctc gcagacgcga
     7741 atggcgcgct tcaggtcgct ggtgtagacg taggccacga gaccgtattc ggtgtcgttt
     7801 gcggcggcta cggcagcttc ttccgagtcg aaggaggcaa caggtgctac ggggccgaag
     7861 atctcctcct tcaggagcgt cgcgtcctcg gggatgtcgt cgaggacggt ggggcggtag
     7921 aagtagccgg acccctccat cttctctgcc ccgatcagcg ccctggctcc cttgccgagg
     7981 gcgtcttcca cgaggccggc gaccttctcg cgctggtcgc cgtcgatgag cggtcctacc
     8041 tcgacgccct cctcggtgcc gcgtccgacc ttcatggcct ccatacgatt ggcgagtttc
     8101 tcggcgaact caccagcgac gccgtcggcg acgtggaagc ggttggccgc tgtgcacgcc
     8161 tcgccgatgt tgcgcatctt ggcgaccatg gcgccctcga ccgcggcgtc gacgtcggcg
     8221 tcgtcgaaga cgaggaaagg ggcgttgccg ccgagctcca tggagacgcg caggagttgc
     8281 tcggccgact gggcgacgag cttgcgcccc acctcggtcg agcccgtaaa ggagagcttg
     8341 cgggcgcgcg ggtcggcgat aattggtccc atcgtgccgc cggaggagga actcgtcacc
     8401 acgttcagga cgccgccggg aaggcctgac tcctccagga tcccggctag ggcgagcatg
     8461 gagagggggg tctgctgggc cggcttcatc actatcgtgc agccggcggc gatggctggc
     8521 ccgatcttgc gcgtccccat cgccataggg aagttccacg gcgttatgaa gatgcaaggg
     8581 ccgacgggct gtttcatagt gagcatgcgc cccgcgccag caggggggac ggcgtagcgg
     8641 ccctctatgc gaacggcctc ctctgagaac caccggaaga actcggcggc gtaagcgatc
     8701 tccgccttgg actcagcgag cgccttgccc atctctagcg tcatcaggag ggctagctcg
     8761 tccgtgcgct ccactatctt ctcgaaggcg cggcggagga tctcaccacg ctcgcgcggg
     8821 gggtgcttcg accactcggc ctgcgcttcg tcggctgccc cgagcgcggc catggcgtcc
     8881 tcggctgtgc cgtctgcgat ctcgcacagt ggttcggctg tcgaagggtc ctcgacgttg
     8941 agtgtgccgc cccctgaggc gtcgcgccac tcgcccccga taaagagctg cttgttcacg
     9001 ctgtctacta cgctttgttc agaggttgcg atgctcatga tctcctcgtg atcgtagact
     9061 gttcctactt tgcaagatat ccgaagaccg ggcggcgtaa accctcacgc cgcccagagt
     9121 aacctcagac ttcgaggttg gtcatcacgt gctttattcg ggtgtagtcc tcgaggccgt
     9181 acatagagag atccttgccg tggcccgagt gcttgaagcc gccgtggggc atctcggcca
     9241 cgagggggat gtgggtgttg atccacacgc acccgaagtc cagcctccgg ctgacccgca
     9301 tcgccctgcc gtggtccttg gtgaacacgc tggaggcgag gccgtacttg acgccgtttg
     9361 cccaccttac cgcctcatcc tcatcggaga aggtctgcac ggtgacgacg ggaccgaaga
     9421 tctccgtctg cgaagcctca tcttcctgcc tgaggccgga gaccacggtg ggggagtaga
     9481 aaaaccccct gtcgccgaac cgctcgccgc cgacggttac ctcagcgtgg tcgggaagcc
     9541 tctcgaccat gcctgagacg tgctccagct ggttggcgtt gtttagcggg ccgtaatcca
     9601 cctcgtcgtc ggggtgccct gtcgtcgtgt tcctggcctg ttcggtgatc gcggcgacga
     9661 agtcgtcgtg gatgccaggg tgggcgagca cgcgggtggc cgctgtgcag tcctgaccgg
     9721 cgttgaagta gccggcaccc gcgatctcct cggctgcctc ctcgatgtcg gcatcgtcga
     9781 aaataatcac cggcgccttt ccacctagct caaggtgggt acgcttgagg tcgtgcgccg
     9841 ccgcctccgc gacctgcata ccggccctga cggaacctgt gatggcgacc atctgcggtg
     9901 tggggtgttg gaccagcatc cgccccgtgt ccctatcccc gcagaccacg ttgaacacgc
     9961 catcgggtag gaactccgcc gcgatctcgg ccatcatcac ggtggtgaca ggggtggtgt
    10021 cggagggctt tatcacgatg gtattgcccg cggctatggc cggagcgaac ttccagattg
    10081 ccatcatcat cgggtagttc cacggtgtta cctggccgat gacacctaca ggctcgcggc
    10141 ggatgaaaga ggtataccct tcgaggtact ccccggcgga tttgccttcc agaacccggg
    10201 ctgcgccggc gaaaaagcgg atctgatcca cccccgccgg gatctcctcc tccatggtta
    10261 gcccgaacgg tttgcccgtg ttctccgact cggcccgcac caggtcctca gcccggtcct
    10321 ctatggcgtc ggccagcctc aggagcgcga gctgcctctc tgaaggcgtc gtatcgcgcc
    10381 acgtctcgaa ggctttgtcc gccgcctcga aagcccggtc gacatcagcc tgctccgaga
    10441 cgggcgcctc ggcgaacgtc aggcccgtgg ccgggttgac cagctcgtag gtgcggccgc
    10501 ctgcggcgtc tacgtgctcc cccccaacga agttcttcag tgttttcgtc tccgtcatct
    10561 ctcctccttg ccgtcgggtt gtccggtatt ctgtcttctc ctgcgcaggg cgatgccgcc
    10621 gagtatctcc ctgatgatcc ggtcggcggc gagggcggtg atgtccgcgg atcccacgcc
    10681 cgtaggaatc acctcgacca tgtcggcccc gatcacctct agttgcatag ccacctcccg
    10741 cacggcccac aacagctcgg cggtagtcat cccaccgggt tccggcgtcc ctgtgcctgg
    10801 cgcaaaggcg gggtccagga cgtctatgtc tacgctgacg aaaacgggtc cgtctcccac
    10861 cgcatccacc gcccggcgga cgacctccct tatgccgagg tctctgatgt ggtgcatgaa
    10921 gaggctggta atgccccgct cggtttgcca ggcgaactcc tccttgccgg gccagtaacc
    10981 gcgcagtcct acctgcgcgt agcgccgcgg gtctacgtgt ccggcctcga cgagcctgcg
    11041 cattatcgta ccgtgcgagg tctcgacgcc gaagacctcg gtgcctgtgt cggtgtgcgt
    11101 gtcgaagtgc accaggccca cgggtccgta cgcggcggcg caggcgcgca cgtcaggctc
    11161 ggagatggag tggtccccgc cgatgatcac cgggatcgcg cccgccgcga gcacctgtgc
    11221 cacggtcgcc tctatcgcgg cgtgggatcg ggtgggatcc gccggtagga ccggcgcgtc
    11281 accgaagtcc acgacccgaa gctcggagat cgcgtcgacc gaggtctcca gttccgggcc
    11341 tgaggggctg ctcgcggcgc ggatggcccg cggcccgaaa cgcgtccccg ggcggtcgga
    11401 caccagatcg tccatcggcg cgccgactat cgccacgtcc acgccctcca ggtgggccgc
    11461 atcctgcgtg tagggaacgc cggcgaaggt gagcaggcca gcgtaatcgg gtttctcgcc
    11521 cacagatgcc cagcgctgga ggggatctag cactacacag cctctacttt accttccaac
    11581 ggcgttggta ccagcaaccc tctgcgccct tgtgcagtcg tgtgggttag ctctttcgtc
    11641 acctctacct ggcacaagcg atccgcgtcc gtcaagcact ctccttgcgc gacatgaaac
    11701 ggctaccgcc caggatcagg accatagtca cgactatcac caccgtcgag agagcgttga
    11761 tcaccgggct cacgccgaac ttgatcatgg agtagatctt caagggcagg gtgctcgacc
    11821 cgaccccgga gacgaagaac gttattacga agtcgtcgaa ggagagcgtg aacgccagta
    11881 gggcaccagc cacgacgcct ggcaggataa gtgggagcgt tacccgcaag aacgtttgga
    11941 cgggggttgc gcccaggtcc tgggccgcct cctctacgga gcggtccatc cccgagagcc
    12001 tggcgcgtac gactatcgta acgaacgaga tgcagaaggc cacgtgggcg atgatgatgg
    12061 tcactatgcc cagttcgacc gttgccgcga cgaagaatac caggaggctg accccgacga
    12121 ctatctcggg catcacggtc gtcgcgtaga tcgccgagtc ggcgagcgtc ttgcccctga
    12181 actcggatcg cgacagcgcg agcgcggtga gcgtgcctat gaccgtggcg atggcggtgg
    12241 ccgtcaggcc gactatgagc gagagcttga acgcgttgag gaccgacccg tccctgatca
    12301 gatccccgta ccactgcatc gagaatcccg tccacacctg ggtgttcttg gtcgagttga
    12361 acgagaagaa gataagaacc attatcgggg cgtagaggaa caggtatacg aggccggcga
    12421 agaggaacag gagccggtcc gggatcttgc cgagacccct gtagacgggc ccgcctttgg
    12481 catcacccat ggcctacacc tcctccagtt tctcggctcc taccctgcgg acgtagaaca
    12541 tgatcgcccc gaccagaaga acggcgagca tgatggcaag cgcgctcccg aaggcccagt
    12601 cgcgggcgct gaggaactgc tgctggatca ggttgccaac catcatggtc cgcgccccgc
    12661 cgagcaagtt cggtatcacg aactccccag cagacgggat gaagacaagg atgctccccg
    12721 ctatcacgcc tggcatgcta agggggaacg tgacgcgcca gaaggtgtgc caccggctcg
    12781 ccccaaggtc ctgggccgcc tctttcaggc gccagtcgaa cttctccagc gccgcgtaga
    12841 gtggcaggat catgaagggc agatacgtgt agaccatgcc caacatcacc gcctgcgggg
    12901 taaagataag ggtcaacggg tagtccgtga tgttcaggaa ctgcagcacc cggttggcga
    12961 tcccgttgtc gccgccgagg atgacgaccc aggcgtacgc cctcaagagc agactggtcc
    13021 agaacgggat catgacgagt aggatgaggg tgtgtttgtg cctaccgccc ttgaaggtga
    13081 tgaagtaagc cagagggtag ccgatcagga ggcagagcaa cgtggtaatc agcgcgataa
    13141 cgagcgtgcg gactatgatc tccagataca gcgggtcgaa gaccttgagg taattggatg
    13201 ggttgaagcc gagtgttatg ccgccgtaca cgccacttgt cccgaacgag tacgccagga
    13261 tgaggagcag cggcacgaag aagaagagcc cga
//
LOCUS       DASTKM010000025         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_531842, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000025 DASTKM010000000
VERSION     DASTKM010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531842"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VFQ10_00885"
     CDS             complement(<1..659)
                     /locus_tag="VFQ10_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9963921.1"
                     /translation="MRLIYTLLLVAVTAVWGWTFVVVQDAISVYGVIPFLAVRFVLAG
                     AALAPIYAAKLTRRSLVVGGGIGVVLAAGYLFQTTGLLFTTPTNSGLITGLFVVFAPL
                     ADRLLFGANVSRQVVLAVALSLVGMVLLAGGGPEGANWGDLLTLLCAAALGLHIALLS
                     RYAASHDAGALTLAQILSIALLFLIVWPFFATVSLPPSEVWVALLVTGLLASAGAFLV
                     QT"
     gene            complement(737..1090)
                     /locus_tag="VFQ10_00890"
     CDS             complement(737..1090)
                     /locus_tag="VFQ10_00890"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9963922.1"
                     /translation="MSEGDTLTGRRRTTVAEAMGRLPGPGGERFAEVLEHGSMTVEIY
                     APRGTDPQTPHTRDEVYVVVSGNGMFVNGPERHPFGPGDVLFVPAGVEHRFEDFTDDL
                     AVWVVFYGPEGGEAS"
     gene            complement(1087..1698)
                     /locus_tag="VFQ10_00895"
     CDS             complement(1087..1698)
                     /locus_tag="VFQ10_00895"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9963923.1"
                     /translation="MWKNLGSVSEGEIVRLEPLARRHEEGLFEAARDERVWRWMHYDA
                     GGSRERFHAWIEDALAASSAGKEAAFATVDAATGELVGSTRYLALRPEHRGLEIGWTW
                     LAPAHWQTGANVEAKLLMLEHAFESLGCLRVEFKTDSRNERSRAALAALPAKFEGIFR
                     KHMLVRGGERRDSAYYSIIDDEWPEVKVNLGRRIDAIRKEEAR"
     gene            2020..>2515
                     /locus_tag="VFQ10_00900"
     CDS             2020..>2515
                     /locus_tag="VFQ10_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9963924.1"
                     /translation="MDHRASGVAMSYRDLGEGDPLVLLHAFPLNGRMFESQMRAFSGS
                     HRVVAPDYPGFGRSPRTPAQPDIHYYAEGVRSLLDRLHLERVILGGVSMGGYVAFECM
                     RLFPERVIALVLANTRPEPDSEETRENRTETALRVADEGVGVLVELQMERLLTPYTLQ
                     NDQEL"
BASE COUNT          480 a          828 c          805 g          402 t
ORIGIN      
        1 gtctgcacca ggaaagcgcc ggccgatgcc aggagtccgg tcaccagcag cgcgacccac
       61 acctcggacg gtggcaaaga gacagtagcg aagaacggcc acacgatgag gaagagtagc
      121 gctatcgaga ggatctgggc cagcgttagc gcgcccgcgt cgtgggaagc ggcatagcgg
      181 gagaggagcg cgatgtgcag accgagcgcc gctgcacaaa ggagggtcag gagatccccc
      241 cagttcgcgc cctcgggccc gccgccggcg agcaagacca tcccgaccag gctcaaggcg
      301 acggccagta cgacctgccg cgagacgttg gccccgaaca ggagccggtc ggcgagcggc
      361 gcgaagacga cgaagagccc tgtgataagg cccgagtttg ttggcgtcgt gaagagcaac
      421 cccgtcgtct ggaagaggta accggcagcg agcaccacgc cgataccgcc gccgacgacc
      481 agcgaccggc gcgtgagctt cgccgcgtag atcggggcga gcgctgcccc ggccaggacg
      541 aagcggaccg ccaggaaagg gatgacgccg tagacggaga tcgcatcctg aaccacgacg
      601 aacgtccagc cccacacggc cgtgacagcg acgagtagaa gggtgtagat cagacgcaca
      661 ggcgcccagt ctacctacaa acgctcagag acgtcggccc tgacgttgtc agggttggtc
      721 gtgagcgcga gatgcgtcaa ctggcttctc cgccctcggg gccgtagaag acgacccaca
      781 cagcaaggtc gtccgtgaag tcttcgaagc ggtgctcgac gccggcaggg acgaacaata
      841 cgtcgccggg gccgaaggga tggcgctccg gcccgtttac gaacatcccg tttccactga
      901 cgaccacgta cacttcgtcg cgggtatgcg gcgtctgcgg gtcggtgccg cggggcgcgt
      961 agatctcgac cgtcatgctc ccgtgctcga gcacctctgc aaaccgctct cccccaggtc
     1021 caggcaaccg ccccatcgcc tcggcgaccg tcgtcctgcg tcgcccggtc aaggtatcac
     1081 cttcgctcat ctggcctcct cttttcggat agcatcgata cgtcgcccga ggtttacctt
     1141 cacctcgggc cactcgtcgt cgatgatgct gtagtaggcg gagtcgcggc gttcaccgcc
     1201 acgcacgagc atgtgcttgc ggaagatgcc ctcgaatttt gccggcaggg ccgcaagggc
     1261 agcgcgtgaa cgctcgttac gggagtcggt cttgaactcg acgcgcaggc agccgaggct
     1321 ctcgaaggca tgttccagca tcaggagctt ggcctcgacg ttggcgcccg tctgccagtg
     1381 tgccggtgcg agccaggtcc acccgatctc cagcccccgg tgctcgggac gaagggcgag
     1441 ataacgggtg ctgccaacaa gctcccctgt ggctgcgtcc acggtggcga aagcggcctc
     1501 cttccctgcc gatgaagcag ccagggcgtc ctcgatccag gcgtgaaacc tctcgcggga
     1561 cccaccggca tcgtagtgca tccagcgcca gaccctctca tcccgcgcgg cctcgaagag
     1621 cccctcctcg tggcggcgcg ccagcggctc cagacgcacg atctcccctt ccgacacact
     1681 ccccaggttc ttccacatgc tccttacccc cgctcaggac ccgtcagaag atatggtagc
     1741 gtacagcggg tcgagggagg gtcagcccta cttcgacccg cgctggtcag caagtcagcg
     1801 gtcagcttct tgtttgctgg ccggctgatg gctcatcgtg cttagggctc gaaccctggg
     1861 ctcggcgctg taggtgtcgg ttaccggaca tcagcgaaag gatggagacg gaaatcagca
     1921 gcccaaaacg ctaccatcgg aggcggcgat ggtctcgcag gagaacgtta ccgcgaggtc
     1981 aaagctgatt tgctgaccag ctaatcaaag gagagcgtag tggatcacag ggcttccggc
     2041 gtagcgatgt cgtacaggga cctcggggag ggtgatcccc tcgtcctgct ccacgccttc
     2101 ccgctcaacg gcaggatgtt cgagtcccag atgagggcat tctcggggag ccaccgggtc
     2161 gtcgcgccgg attaccctgg attcgggcgt tctcctcgta ctccggccca gcccgacatc
     2221 cactactatg cggaaggagt gcgcagtctc ctagacaggc tgcatctgga acgcgtgatc
     2281 ctcggcggcg tctccatggg cggctacgtc gccttcgagt gcatgaggct cttccccgag
     2341 agggtgatag ccctcgtcct cgccaacacc cgccccgaac ccgactccga ggagacgagg
     2401 gagaaccgta ccgagacggc cctcagggtc gccgatgagg gggttggggt cctcgtggag
     2461 ctccagatgg aacgcctcct caccccctac accctgcaga atgaccagga gctgg
//
LOCUS       DASTKM010000026         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1237306, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000026 DASTKM010000000
VERSION     DASTKM010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237306"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFQ10_00905"
     CDS             <1..731
                     /locus_tag="VFQ10_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HET9963925.1"
                     /translation="YQKSLCHILLAPVGIGTAINVLAVLAGGSIGTLAGARLPEGMRS
                     TAMQAIGIVTLLVGVSHFLRADNVLVPLLSVILGLVVGELLDTDARLRRLGDHLEERF
                     SKGESPVSRAFVTTSLLFCVGPLTVIGSLQDGLNGDYSLLVLKSALDFIAALAFASVL
                     GWGVLLSAGTVLVVQGSLTLGAGFLDAVITGPMISSTTATGGVLIFGLGLGLLDLKEV
                     RVANMLPALLFAPLLVAASPLWPL"
     gene            824..1045
                     /locus_tag="VFQ10_00910"
     CDS             824..1045
                     /locus_tag="VFQ10_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963926.1"
                     /translation="MEGTDKQWRGSPLDAAGIGMDAYGIKEIVIEFDNGDEDIFTPKQ
                     REVFESYELHQMATYLDSIGHSIRKGKKN"
     gene            1057..1281
                     /locus_tag="VFQ10_00915"
     CDS             1057..1281
                     /locus_tag="VFQ10_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963927.1"
                     /translation="MHGIERTRKAHGPMEQGVGVTRMVVRYSDGRVMNFVPDAGRDAF
                     SEDDMLEMKKVLERISSTAEWAEINTRLGF"
     gene            complement(1298..1540)
                     /locus_tag="VFQ10_00920"
     CDS             complement(1298..1540)
                     /locus_tag="VFQ10_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963928.1"
                     /translation="MAQGVMRKIGELSEERERLLVREGTHHADSGDRERLVQIDHDLQ
                     VLWDLRRRELAGERIDLDEDFLDRYTVDPGRDAPGR"
     gene            1636..2043
                     /gene="mce"
                     /locus_tag="VFQ10_00925"
     CDS             1636..2043
                     /gene="mce"
                     /locus_tag="VFQ10_00925"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HET9963929.1"
                     /translation="MLNRIYHLGYAVEDIEAASRFYEENFGAVPGEPEVVEEQGIVAT
                     MFKVGESSIELVQPTRPDSPVGKFLEKRGEGFHHVAFEVEDLSVTLAELKSNGVEVID
                     EEPRRGAGGTRMAFVHPRGAFGVLTELVELPHA"
     gene            2065..3663
                     /locus_tag="VFQ10_00930"
     CDS             2065..3663
                     /locus_tag="VFQ10_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9963930.1"
                     /translation="MDTEKRQDGRRTESGIEVKPLYKPEDLEGFDYPERLGDPGEYPY
                     TRGPYPSMYRGRPWTMRQYAGFGTARETNARFKYLIDNGQTGLSVAFDLPTQMGRDSD
                     HALALGEVGKVGVAIDSLLDMRDLLEGIPLSDVSTSMTINATASILLLLYSLVAEEQG
                     VEAEDVTGTTQNDILKEYLARGTYIYPPAPSMRITTDMFAYCAAELPRWNTISISGYH
                     IREAGSTAVQELAFTLSNAIAYVEAALDAGLEVDEFAPRLSFFFNAHNDLFQEVAKYR
                     AARRMWARIMRDRFGATDERSLKLRFHTQTAGSSLTAQQAENNIVRTTVQALSAVLGG
                     TQSLHTNAFDEALALPTERSARIALRTQQILAGEGGLTGTADPLAGSYYVESLTDAVE
                     ESAWAYIRRIDEMGGSVKAVEERFMQHEIEEAAYRYQREIEREGRVIVGVNRYTSGGT
                     DDPDIELHTVDETIRERQKERLAHLKEIRDNVAVEKSLAHLKDAAEGTENLLYLMKEA
                     LAELATLGEVSDALRGVFGEYRPS"
BASE COUNT          772 a         1204 c         1276 g          679 t
ORIGIN      
        1 ggtaccagaa atccttatgt catatactgc tggctcccgt gggaatcgga accgcgatca
       61 acgttctcgc cgtgctcgcc ggcgggagta tcggcactct cgcaggcgca aggctacccg
      121 agggcatgcg ctcgaccgcc atgcaggcta tcggtatcgt aacgttgctc gtcggcgtct
      181 cgcatttcct gagggccgac aatgtgctcg tgccgctact cagcgtgatc ctgggactcg
      241 tggtaggcga gcttctggat acggacgcgc ggctacggcg gttgggagac catctggagg
      301 agaggttctc gaagggcgag agtccggtca gcagggcctt cgtgacaacg agcctcctgt
      361 tctgcgttgg gcccttgacc gtgatcggct cgctccagga cggcttgaac ggagactaca
      421 gcctcctcgt cctcaagagc gccctcgact ttatcgcggc gctggccttc gcctccgtac
      481 tggggtgggg cgtgctgctc tcggccggaa cagtcctcgt ggttcagggc tcactgacac
      541 tcggggccgg gtttctggac gcggtcataa ccggacccat gatctcgtcg accaccgcga
      601 ccggaggcgt cctgatcttc ggcctcggcc tgggcctgct cgacctgaaa gaggtcaggg
      661 tagccaacat gctccccgcg ctccttttcg ccccactgct cgtggctgcg agtcccttgt
      721 ggccgctctg acttacacgt gcgccgccga tactctagta ttcgtaggag gaacgagagt
      781 gcaatgaccg tgcagaagtt cgcgcggtgg gaaggagcgg ctgatggaag gaacggacaa
      841 gcagtggcgg ggcagtccgc tggacgcggc agggataggc atggacgcct acggcataaa
      901 ggagatcgtc atcgagttcg acaacggcga cgaggacatc ttcaccccga agcagcgcga
      961 ggtgttcgaa tcctacgagc tgcaccagat ggcgacgtac ctcgactcga ttggccacag
     1021 catccgcaaa ggcaagaaaa actaaagggg gacggattgc acggcataga gcggaccagg
     1081 aaggcccacg ggccgatgga gcaaggcgtc ggggttacgc gtatggtcgt ccgttactca
     1141 gacggacgcg tgatgaactt cgtacccgac gctggccgcg acgcgttctc tgaggacgac
     1201 atgctggaga tgaagaaagt cctggagcgg atatcctcga cagccgaatg ggccgagatc
     1261 aacacacgcc tggggttcta gtgtaggggc gccgccccta cctgccaggt gcgtcgcggc
     1321 ctggatcgac cgtgtagcgg tcgaggaaat cctcgtcgag gtcgatcctc tcgcccgcca
     1381 gttccctacg cctgaggtcc cagaggacct gcaggtcgtg gtctatctgt accaggcgct
     1441 cgcggtcacc tgagtccgcg tggtgcgtac cctcccttac caggagtcgc tcgcgctcct
     1501 ccgagagctc ccctatcttg cgcatcacgc cctgtgccat gcttgcctcc tacttattga
     1561 cctccttgat tctagcggat atggatagac tacaatcagg gttcccgacg ccggagtgcg
     1621 agcaggggga gaagagtgct caacaggatc taccatctcg gttacgcagt ggaggacatc
     1681 gaggccgcct cgaggttcta cgaggagaac ttcggggccg tgcccggaga gcctgaggtc
     1741 gtagaggagc aggggatagt ggccaccatg ttcaaggtcg gggaatcgag catcgaactc
     1801 gtccagccga cccgcccgga ctcgcctgtc gggaagttcc tggagaaacg cggcgagggc
     1861 ttccaccacg tcgctttcga ggtcgaggat ctcagcgtga cgctggctga gctcaagagt
     1921 aatggcgtcg aggtcataga cgaggagccg aggagggggg caggggggac cagaatggcc
     1981 ttcgtccacc ccaggggtgc cttcggggtg ctcaccgagc tagtagaatt gccacacgcc
     2041 tgagacgagc ggggaggacg agccgtggac accgagaaac gccaggacgg gcgccgtacg
     2101 gagtccggga tcgaggtcaa gcccctctac aagccagagg accttgaagg cttcgactac
     2161 ccggagaggc tcggagatcc gggggagtac ccttacacgc gcggtcccta cccgagcatg
     2221 tatcgcggca ggccctggac catgcgccag tacgcgggtt tcggcactgc gcgcgagacg
     2281 aatgctcgct tcaagtacct catagacaat ggccagacgg ggctctcggt ggcattcgac
     2341 ctgcccaccc agatggggcg cgactcggac catgcactcg ccctcgggga ggtgggcaag
     2401 gtgggcgtgg ccatcgactc cctcctggac atgcgcgacc tcctcgaggg aatcccacta
     2461 agtgacgtct cgacctcgat gacgataaac gccacggcct cgatcctatt actcctctac
     2521 tcgctcgtcg ccgaagagca gggagtggag gctgaggacg tcactgggac gacccagaac
     2581 gacatactca aggagtacct ggcccgcggt acctacatct acccgccagc gccatcgatg
     2641 aggatcacca cagatatgtt cgcctactgc gccgcagagt tgccgcgctg gaacaccatc
     2701 tcaatctccg gctaccacat aagggaagcc ggatccaccg ccgtccagga gctggctttc
     2761 accctctcca acgccatagc ctacgtcgag gccgccctcg acgcggggct ggaggtggac
     2821 gagttcgcgc ccaggctctc attcttcttc aacgcccaca acgacctgtt ccaggaggtg
     2881 gccaagtaca gggcggcgag gaggatgtgg gcccggatca tgcgcgatcg cttcggcgcg
     2941 accgacgaga ggagcctgaa gctcaggttc cacacccaga ccgcaggctc ctcgctgaca
     3001 gcgcagcagg ccgagaacaa catcgtccgc accactgtcc aggcgctctc cgcggtcctc
     3061 ggcgggaccc agagcctgca caccaacgcc ttcgacgagg ccctcgcact cccgacggag
     3121 cgtagcgcgc ggatcgccct gagaacccag cagatcctgg cgggcgaggg tgggctgacg
     3181 ggaacggcgg acccgctcgc cggctcctac tacgtcgagt cgctcaccga tgcagtcgag
     3241 gaatcggcct gggcctacat aaggcgcatc gacgagatgg gaggctcggt aaaggccgtc
     3301 gaagagcgct tcatgcagca tgagatcgaa gaggccgctt accgctacca gcgcgagatc
     3361 gagagagagg gacgcgtcat cgtcggcgtc aaccgctaca cctcgggtgg gaccgacgac
     3421 cccgatatcg agcttcacac cgtcgacgag acgatccgcg agcggcagaa ggaacggctc
     3481 gcgcacctca aggagatcag ggacaacgtc gccgtcgaga agtccctcgc ccacctgaag
     3541 gacgcggccg aaggcaccga gaacctcctc tacctgatga aggaggctct ggcagagctc
     3601 gcgacgctcg gcgaggtctc cgacgcgctc cgcggggtct tcggagagta caggccttct
     3661 tgaggcccgg ggtctttgcg cccgactatg gaggccagga tctcccgcgt gcgctcttcg
     3721 tagataggtc gaagggtgcg catggaggta tgttgaccct gtgttaacat tggcacgaag
     3781 caggaagtat ctaccgcact ttcgagctag ttagagtcgc cggtccgtgg tgccggagga
     3841 ggttggcaga cgtggttgat tggtttcgca gacgtaaggc agggtcatcg gacgatgttg
     3901 accggccggc gcatctcgag gccgagacgg c
//
LOCUS       DASTKM010000027        30041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_708913, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000027 DASTKM010000000
VERSION     DASTKM010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 30041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30041
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708913"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1813
                     /locus_tag="VFQ10_00935"
     CDS             44..1813
                     /locus_tag="VFQ10_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET9963931.1"
                     /translation="MPNTNGHGLKRAVLWAVLYARVSTDEQARSGYSLAQQLEALRAY
                     AAREGYEVLEEVQDPGQSGASLERPGMDRVRDLVAGGGVSVVLAQDRDRFSREPAYTY
                     LLRREFEEHDCELRSLNDRGDGSPEGDLTDGILDQLAKYERAKIAERSRRGKLRKARE
                     GKLIPSPTPDYGFDYNGTRDNYVVDEEKMQVVRRIFRMIGAEGDALNGVKKTFDREGI
                     PTPRGSRYWGNTFIQRVVTDDVYKPHTFEEVKALVSPEVASRLDPDKSYGVWWFNRRR
                     RTEKQVSEIASDGRRRYVRRSTTTQKPKEEWVAIPVPDAGIPREWVDRAREAVSDNVK
                     FSRAGGRFWELSGLMKCSECGRGMLGNSPPNASRKKVYHYYRCRTRHMEGTEACSMWK
                     NIRAGEAERAVWSFVTDLLLNPDALREGLDEMMRRELATNQGNPEEEAAVWFDRLVEA
                     DSRRANFQDMAAAGLITFDELGAKLDALEEARKTARRELAAIEGRKELLRAIERDRAA
                     LLEGYAGLVPEALDDLDSEERHRIYNMLRLRVSAFPDGTLEVGGTLSDGHLVCKKSTR
                     RLLSRGTSRVRSRRSSRGRPSAT"
     gene            1762..2121
                     /locus_tag="VFQ10_00940"
     CDS             1762..2121
                     /locus_tag="VFQ10_00940"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9963932.1"
                     /translation="MKSKVEEVEPGKAVCHIDVESIHLNGNGTLHGGVYASLIDNAMG
                     LSVAALVGLRTATIGLDVHFLGAVREGRITCSAGVVHRTRRIATVEARVRDGDGNLVA
                     MGTGTFRIFEKRGDPIV"
     gene            2290..3942
                     /locus_tag="VFQ10_00945"
     CDS             2290..3942
                     /locus_tag="VFQ10_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET9963933.1"
                     /translation="MFRKERPWLGVYEKGRVEPETQIFEGSLYELFSGAVEEHHGKTA
                     LSFYDTTFEFGRLQALVEKMAASLAASGVEKGDRVALMLPNCPQYVVSFFATVRLGAI
                     VTQINPMYVEREIEHILNDSGAETIVVYADVYERVKALLPDTNLKTVVVVDFVGEQEG
                     LNAGHRYFGDFLATDDEPAPEVDIDPAGDVAALQYTGGTTGVSKGAMLTHRNLVANVQ
                     QTIDVFVRDPAQFTGRKCVGALPFFHIYGLTCVMLFGIRLGIEQVLLPRFEVQEALAV
                     FEKDRPTMFAGVPTMYMALLASGADLRKHHLHDVQIFNSGGSALPVNLKRSFEEKVGR
                     TLFEGYGLSEASPVTHNNPPFLGQGREGSIGIPIPSTEARVVDVETGETEMPIGESGE
                     LIIKGPQVMKGYLNMPDETAETLKGGWLYTGDVAKMDESGYFYIVDRKKDMIVASGYN
                     VYPREIEEVLFEHPDVAEAVAIGVADEYRGESVKAFVVKRSGASTTEEEVLAFCKERL
                     AAYKTPKAVEFREELPKSAVGKLLRRVLVDEERAKTEATPRS"
     gene            3955..4731
                     /locus_tag="VFQ10_00950"
     CDS             3955..4731
                     /locus_tag="VFQ10_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9963934.1"
                     /translation="MNALDLFRLDGSTAIVTGGGRGLGRYMAEALSDAGAGVVLCSRK
                     IEPLEEVRSEIEAGGGRALAVECDVTDPEDVERVVSQAEEAFGSLDIVVNNSGATWGA
                     PPAEMPLERFDQVLRVNVRGTFLMSQAAGRRMIERGSGGTIINISSVAGLVGGHPDYM
                     QTVGYNSSKGAIISMTRDLATSWAPHGITVNAIAPGWFPTRMSGGLIEKFEERMLQGI
                     PLHRFGNPDDLKGVVIFLASPAAAYVTGQTLVVDGGATAW"
     gene            complement(4789..5601)
                     /locus_tag="VFQ10_00955"
     CDS             complement(4789..5601)
                     /locus_tag="VFQ10_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9963935.1"
                     /translation="MPYATNALDGSRVYFEDDGGDGAPVVLHGGIIDSVDLVRESNIA
                     QALQELPTNEFRLVYVAHRGVGRSDKPHEVEAYAMPLRVADAVAVLDELGIERAHFIG
                     NSWGGRLGFGIGEHAPERVLSLVIGGQQPYAIDPDGPLAHAATETLAESRAEGSMEPF
                     VEMLESFAGTRFPDALRERWLDNDPAAIEAAWSAGVAEGDISEDLGAWELRCLIYVAT
                     GDVDFHDQAQQAANEIPNAEFNSFDESDHVGAYLAEIDPVLAAVLRTLRASG"
     gene            5853..6608
                     /locus_tag="VFQ10_00960"
     CDS             5853..6608
                     /locus_tag="VFQ10_00960"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963936.1"
                     /translation="MATIDGWLEGFLHGPAALWVILLVSLALGLRHASDPDHLAAVTT
                     LIASESGYGVRKAGSMGLSWGIGHGTSLVLIGLPLVLLGRYLPEPVQQVAEALIGVII
                     IILAARLLVRWRRGHFHAHVHSHSGEPVHRHVHSHESDSSHGHAHHVFHRTPLSSYGI
                     GLVHGIGGSGGLTLLLLSTIPGPGQATVALLIFAGGTAVSMALLSTAFGLAIASGPIG
                     RNFERLTPALGASSLAFGTWYMLGALGVVAYPL"
     gene            6774..8183
                     /locus_tag="VFQ10_00965"
     CDS             6774..8183
                     /locus_tag="VFQ10_00965"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HET9963937.1"
                     /translation="MQPLTLRAGKRQLVLILFALAISAMALGVVGISKPADASSHREA
                     PFISGDPQADATDLYAFRSPDRPNSVTLIANYIPAEVAAAGPNFYRFGGDVLYEINVD
                     NDADARDDLSFQFRFQNRTNNGNTFLYNTGPITSLNDQNLNQEQFYSVSMLKGMDHEE
                     ALREGKVIAKNLQVAPANVGPVSYPEGYRKVANQAIYDIGDGVKVFAGPRDDPFFADL
                     GGIFDLLQGIEGEDYLADLNVHTVAIQVPIDKLTTGDSNIIGVRTTSYRQSMSVLRPI
                     GQPGSQNNNPSSSSGPWVQLSRLDMPLVNELVIPLKDKNRWNGSEPRFDSQFAKYVLD
                     PEPARLIESILGVDVPNPPRNDLSTIFLTGIPDLNKPAGVKPSSQLRLNTAIQPSDNP
                     ERLGVLRGDNAGFPNGRRPADDLVDIELQALAGGTPLTPGFNKAPNNQLGDGVDANDV
                     PLRNSFPYLADPQDYTETE"
     gene            8261..9595
                     /locus_tag="VFQ10_00970"
     CDS             8261..9595
                     /locus_tag="VFQ10_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9963938.1"
                     /translation="MTVIRRSRLLWALIPVLAALATLATLALLRGERSADQRVELPFD
                     FRQTAGTSTDSLIGSLQEKIRDNPKDFDSHINLANAYLQKVRETGDPTLYTRTEDLLG
                     NARKLEAHSPELFATRGSLDLARHDFAAALQNGTRALALDPENARYHGIVGDAQIEQG
                     MYEEAINSYQKMVDRRPDFDSFSRVAYARELYGDPEGAIEAMEFALQAGSGTPENVAW
                     AHVQLGNLWFTSGKLEEAQKAYTLSTRTVNAYAPALAGQAKIAAARGDLEQAATLYKQ
                     AFNRMPLPEYAIALGDVYAAMGERVKAEEQYDLVRNMDKLLRANGVDTDLEIALFNAD
                     HDIDLRMSLEKARAAYEARPSIHAADTLAWTLYKTGNYKEAREYSSEALKLETRDPLK
                     LFHAGMISKALGEKEQASTYLQQAVDLNPHFSLLYADEAAYTLKTLKAGGEH"
     gene            9595..11085
                     /locus_tag="VFQ10_00975"
     CDS             9595..11085
                     /locus_tag="VFQ10_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963939.1"
                     /translation="MVRWGWPLACLAFAVFASLIPREAQAHPLGNFTINHYSRLEFAE
                     ETARITYVLDFAEIPTLQQKARLDANGDGELSGKESNAYLDAEMPSLVEGIRLRAGHE
                     VLPLEVLDRSAEYRSGQGGLPTLRVETHLLADLPKDWRGEAHYTDQTYANRLGWREIV
                     VRGGPEMAIRNSTVQANSVSDELRSYPRDMLSSPPDVREASFTLAPGDGSVHNDAAGR
                     TAEHGGTSFGGVTGRIDALVSVERLSAAVVALAMLAAFFWGAAHALTPGHGKAVVAAY
                     LIGARGTARHAGILGLTVTLTHTAGVFVLGAVTLYLSRYILPEVIYPWLSVASGLLVV
                     LIGLALLYRHLDKMFHDRKAEHTHPHDGAHSPGGSRSDVHHTHSHGGHSHLPVDGSKI
                     TWRGLVALGVSGGLVPCPAALVLLLGAISLDRLGFGMVLVLAFSAGLAIILTGIGLLM
                     IYARKLFERFTFEARVPRLLPVVSASIITLAGVGVALGAVQQTGIV"
     gene            11314..11565
                     /locus_tag="VFQ10_00980"
     CDS             11314..11565
                     /locus_tag="VFQ10_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963940.1"
                     /translation="MKTGTSVGIACAVGVVGGVMIGSRAVKIYDWRTEWTIPAPMPVV
                     YEAMTSRQAVREWWPDMELAEDNGDDGLQVGSTVSFRVH"
     gene            11653..11877
                     /locus_tag="VFQ10_00985"
     CDS             11653..11877
                     /locus_tag="VFQ10_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963941.1"
                     /translation="MSGDLSGVLETLFHEQHDGTSITFNWYVRLTNPALNLLGYAAER
                     VYRHSHDSVMTSGEKGLSEYCKRRAASGRT"
     gene            12199..13530
                     /gene="trmFO"
                     /locus_tag="VFQ10_00990"
     CDS             12199..13530
                     /gene="trmFO"
                     /locus_tag="VFQ10_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET9963942.1"
                     /translation="MPNVTIIGAGLAGSEAAWQAAELGCSVELWEMRPRRETPAHRTE
                     HFAELVCSNSLGNLALETASGLLKEELRRLGSVILRCADANTVPAGGALGVAREDFPR
                     AVTETIHAHPNIEVIRKEVTDIPQGPTVVATGPLTSDALAKKIEDLSGETLYFYDAAS
                     PIIHRDSLDDSIIYRASRYGKGEAAYLNCPMTEERYYAFVEDLAAAELSPIKDFEEDL
                     YFEGCLPVETIARRGKDTLRFGPMKPVGLPDPRTGEMPYAVVQLRQDDAEGCLYNIVG
                     FQTRLKWGEQRRVFRTIPGLQYADFARMGVMHRNTYLPSNHMLDATMRIRKALGEAPL
                     FFAGQLTGVEGYVESTAMGHIAGTNAARLARGEEPITMPRGTMMGALAHYVTTKEGTL
                     QPINSNWGLVPTVPKRENGRRLSKSERRQRQAWMALSRLDEFMREGIKTAV"
     gene            13617..13880
                     /locus_tag="VFQ10_00995"
     CDS             13617..13880
                     /locus_tag="VFQ10_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET9963943.1"
                     /translation="MKAITTYLSAGHIGAFIKLFPVVSWLVKERRELAVIRQIPTHER
                     LGIAFAKTNVALCKAVNKNLASIRERGLFDALRRKWLPESDKD"
     gene            14023..14268
                     /locus_tag="VFQ10_01000"
     CDS             14023..14268
                     /locus_tag="VFQ10_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963944.1"
                     /translation="MSETMSDKEIPETWVGQRVRVGHAHSASTHTDGLLLGVGDRGIV
                     VEGGELVRFYPWTSVGSIEKTHEEGGENPGVQAGRAW"
     gene            complement(14322..15062)
                     /locus_tag="VFQ10_01005"
     CDS             complement(14322..15062)
                     /locus_tag="VFQ10_01005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9963945.1"
                     /translation="MSTGHPKLYAELAGWWQLISPTEDYADEAAFFGRLFRAENAGTV
                     LELGCGGGNVAWFLKEDFALTLTDISEEMLAESRKQNPELEHIEGDMRTLRLDRTFDG
                     ILIHDAIMYMSSEEDLRAALLTAYEHCKPGGVAVFVPDWVAETFRPGTTHEGVDRGDR
                     GVRYLEWTWDANPNDTKVNYEFILALKEGDELRAVVDRQVVGIFPRATWLRLLEDVGF
                     EPEVVEDPSSGGERVEVFVGHKPLPPTG"
     gene            complement(15108..16064)
                     /locus_tag="VFQ10_01010"
     CDS             complement(15108..16064)
                     /locus_tag="VFQ10_01010"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET9963946.1"
                     /translation="MARVAVLRGGHSMERDVSFITGRRVQHALERLGHDVYPLDIEDT
                     TTGTLMEISPDAAFICLHGPGGEDGTVQALLETLNIPYTGSGPLSSIRCMDKDYAKRA
                     LRAAGIQTPPFRTFLRRAMNEMGASAALDVAAEELGYPLVVKPAREGSSFGLSRVEGA
                     EGLFDAVYEALGYDAKILLERWVEGTEISIPIIEPAGEEPKALGLVEIRPREGAYNFE
                     AKYTPGATDFAIPAEIPSEAASRVEETALTIYKILGCSGFARVDTIFAEGTPWVLDVK
                     TIPGFTETSTFPLAAETAGTTFEMLVETILDAALRTEVTAKL"
     gene            complement(16071..17396)
                     /locus_tag="VFQ10_01015"
     CDS             complement(16071..17396)
                     /locus_tag="VFQ10_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9963947.1"
                     /translation="MPLVDLDRFDFLYANRVKGMKSAATRDLMATLSRPGIISLAGGF
                     PDTKAFGEEAFREISRNIASDAAQALQYGPTAGLEAIKDVIVEVMGAEGTPARQEDVF
                     VTTGAQQGLDLIAKVFLDEGDAVLCEGPTYAGALNAFAAYRPRIAHAPMDRAGIIPVA
                     ARETLKNARKGGLHVKFLYTVPNFQNPAGVTMVAERRRELLEMAREFDLVIVEDNPYG
                     MLRFEGEPLPSLAALEQEEGDLDRVVYLGTFSKIFAPGVRLGWVHAQPGILHKINVGK
                     QGADLCSSNLSQMMISSYFQNADWRAYVGRLTSMYKERRDAMLDSLAEFMPKEVYWTH
                     PEGGLFVWATLPSYLDATAMLPRAIARNVAYVPGEGFYGGAPGTGKNNMRLNFSFVEP
                     KRIRRGIELLSEVIRERMELRSDLERGSHRKEGAIHGTRAVGFSSGTEG"
     gene            17516..18079
                     /locus_tag="VFQ10_01020"
     CDS             17516..18079
                     /locus_tag="VFQ10_01020"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HET9963948.1"
                     /translation="MKAIFVTTNEHKRREVQEILGFELERADLDLPEIQAIDPAEVAA
                     DKARAAREVLGRPGLPVLVEDSGLMVDAWDGFPGALTKWLMQSIGSEGLLRMLPPGED
                     RSARAVCVVALAQADGNVLTFRGEVPGTLAQRPRGTGGFGYDPVFIPEWSSMTYAEMG
                     EGKNEDSHRARAFRAVREWLGEAWSGV"
     gene            18162..19226
                     /gene="pdhA"
                     /locus_tag="VFQ10_01025"
     CDS             18162..19226
                     /gene="pdhA"
                     /locus_tag="VFQ10_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET9963949.1"
                     /translation="MSADNEHSEDGGVETREGGGAIATSSERLVDLYRQMALIRAFEE
                     ACQRGFRQGKIGGYLHVYIGQEAVATGFLEAFKEGDKVITAYRDHAHALLLGSDPKEI
                     MAELYGKETGLVKGKGGSMHLFDVERGLMGGYGIVGGHIPLGVGIAYAMRYQETDHIT
                     QLYLGDGAMSQGAFHEAANLAGLWGRDGQCPCLFIVENNKYGMGTSVERTTAMTDLAA
                     KFDAYGIENEKVDGMDVETVIEVAERVAEQVRETGKPYAVEALTYRTAPHGAADFFEK
                     YRTKEEIEEWRERDPIAMVEHKLIENGALDEEKLEEIRGEAKERVQEAVKFAEESEEP
                     PEEELYTDVYSGKDEYMGEE"
     gene            19230..20219
                     /locus_tag="VFQ10_01030"
     CDS             19230..20219
                     /locus_tag="VFQ10_01030"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET9963950.1"
                     /translation="MAETKTYREALREAMVHELDRDENVVLMGEDIGVYGGTHLITDG
                     LYDEYGPRRIMDVPISENGFTGAAVGMAMMGMRPVVEMMTWNFSFLAADQIIQNSAKV
                     RYFSGGQVEVPLVIRGPNGGGVQLSAQHTHSLESFYGHFPGLKVVAPVTPNDVKGMML
                     TAIRDPNPVIFLEAGALYGTKGEVEEGDNAVPFGEARIAREGSDVTLIAYGRQVNLCL
                     RAADTLAEEGFEAEVIDLRSLRPFDEDTIVESIEKTHRAVAVQEQWRWFGVASEVAAI
                     IQERAFDSLDAPVERVSGAEVPGPYARNLELMAFPSEKQVTNAARRVLYEEEK"
     gene            20220..21560
                     /locus_tag="VFQ10_01035"
     CDS             20220..21560
                     /locus_tag="VFQ10_01035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET9963951.1"
                     /translation="MPEVIMPKMGDAMEEGTLLKWLKSEGEEVSEGDPIAEIETDKVT
                     MELEAEDEGTLAQLIADEGQEVPVGDAIAFIQGEGEEVPERSEGGGEAAEEAEEGGEE
                     DGGGAQATATETEAPEEGETEEADGRADGRADGHFRASPIVRRLAEENDLDLSKIDGS
                     GPAGRIVERDVRAAMESGTAQRTEDGAAGGVAAPEQPQQAEMQDFQPARLPEPTEEPG
                     TRLVEPTRMMQVIGERMTEAKQHIPHFYATVEVRMDAAMALRKQLNAQLEDEGIKLSV
                     NDFVMKACAVALRSYPNLNALYTTRGVELHEKVDMAMAVALDQGLITPVIRDIGSKGL
                     AAISREAKDLAMRAREGKLQPDEYQGGTITVSNMGMFGIESFTAIINPPQAAIVAVSS
                     ITKRPDYDENGEIVPASMMKLTLSADHRIANGRDGALYMAEVKRVLENPVMLMV"
     gene            complement(21520..21855)
                     /locus_tag="VFQ10_01040"
     CDS             complement(21520..21855)
                     /locus_tag="VFQ10_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9963952.1"
                     /translation="MTHRLTPLAPDHTHVECEWLFSPEAVEQEDFDPSYASDFWDVTN
                     RQDWTACEAVQRGVSSRGYRRGPLSPREDAVYQFISMVANGYLADGVARPDSRAPTPS
                     TSRGFRAPA"
     gene            complement(21855..21998)
                     /locus_tag="VFQ10_01045"
     CDS             complement(21855..21998)
                     /locus_tag="VFQ10_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963953.1"
                     /translation="MTPISCELLAPSLDTFGRSSTLPAEAYTTGSVIRWEQEHFFDFT
                     PTR"
     gene            22095..23744
                     /locus_tag="VFQ10_01050"
     CDS             22095..23744
                     /locus_tag="VFQ10_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9963954.1"
                     /translation="MASDGLRYDAVVVGGGHNGLVCAAYLARAGMKTVVLERRHLVGG
                     AAVTEEVWPGFGVSTASYVVSLMSARVVRELDLSRYGYHVYPLDPAYFAPFPDGSGFL
                     LWDDPGLAAEEIGKINVRDGQAYLEYNRELGELADLVRPLLYRRPPDPGIRSLADVVE
                     ALSLGLYAFGNRRSISRLVDLMTMSCADFLDRFFVDERVMGALAPGGVIGMWGGPMSP
                     GSAYVLLHHRMGEIDGVTGGWGFVRGGMGGVSEAIAASARAVGTEIRTNAEVAAIDVK
                     GGRATGVTLADGARIEAGTVVSGAHPRTTLLSLLGAEHLPDELVRELETFRNRSPSAK
                     VNLALSELPDFTAMPGKELGPQHPETMISPSLEYLEKAWDDAKYGRSSEGPMIDAVIP
                     TTKDPTIAPEGRHIMTCFVQYAPYEPREGRWDGGAREALGDKVVNTVTEYAPNFKDLV
                     IERQVLTPYDLEQRFGLIGGNIFQGEMSLDQLFSFRPAPGLAGYRTPVHGLYLCGSGT
                     HPGGGVMGIPGLNASKVVIKDHKSAERKRRLKERLASYSRR"
     gene            complement(23856..24656)
                     /locus_tag="VFQ10_01055"
     CDS             complement(23856..24656)
                     /locus_tag="VFQ10_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET9963955.1"
                     /translation="MLDSLRNLLDPLFNLMGGTLLTFHGWGAPWWLAIVMLTIVVRTL
                     LFPITYRQVKSMRRMQDLKPDMDRIRAEYKDDVQKQREEIAKLYQERQVNPLGGCLPI
                     FIQLPIFLVLYYTIRHFDKLESFRTGGLFWFKDLTVYDHLFILPVLYVLTMMASQELS
                     IRNTAAQQKRLMRFLPIIFGFFLARFPSGLFVYWVTSNLITFTQNYVIYNVLPHKPVD
                     TEGVKSPIEAEKESTVAKQEQEKREIPQNRKKSGRAKNRKEKKVGKRK"
     gene            complement(24726..25055)
                     /locus_tag="VFQ10_01060"
     CDS             complement(24726..25055)
                     /locus_tag="VFQ10_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963956.1"
                     /translation="MRGVFSPAFGLVLLGLLVNFFSREEVYANFEYGFPMLALFGAAG
                     CIGQLTVSISRRSGTLASACCIPIWVLRARVVKDSVRGETSDLPVLVALLFCPLVVLL
                     AGLGGWV"
     gene            25302..27344
                     /gene="fusA"
                     /locus_tag="VFQ10_01065"
     CDS             25302..27344
                     /gene="fusA"
                     /locus_tag="VFQ10_01065"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9963957.1"
                     /translation="MAVETETIRNVCLIGHRGSGKTSIAEGMIGLASGRSGPTSGVLD
                     YTDEETERGMTLGMSVANLQWKGRQVNVLDTPGDGGFVGDAFIAQRVADCAILVVHAQ
                     DPIQVVTERVWRRGEKEGIPHVIVVNHLDRERTDFGAVVDELRERFGQPVVPLNLPIG
                     RENDLKGIYGLLSGTSFVDGEQRAEIPEGMEDEVDEAKTQLFEAIAESDDTLLEKYLE
                     GEEITTEEAFDGIRKGIAEGLIIPVLAASAERMIGIDRLLDVIAGSAPSPSDLERWIT
                     EESEEIPCDPEGPFAAYVFKTYVDPYAGRLSVLRIVGGRCRSDEALTNPRTDSSERLG
                     GISHLVGKEREPVDEAVAGDIIAVAKLRDTATFDTLCKPEKPMSFEPVELPEPTAAFA
                     VGAKARGEEEKVFDAIRRVVDEDPSLKLERTEATGEDILSGLAQMHVELALERIEHRY
                     GVEVEARPPKVPLMETITGSAQAQGRYKKQTGGRGQFGDARIELSPLPRGSGFEFENG
                     IVGGAIPRQFIPAVEKGIIEAMAGGNIAGYPVVDVKVRLHDGAFHSVDSSENAFKVAG
                     SMAFKNAVEKAHPVLLEPYVKVEVLTPSELVGDIMGDLSSRRGRPMGVEQRGERQIIQ
                     AEVPQIEMLTYARDLRSITGGRANFHVEAGHYEEVPPNLVEKVLAANEREEEKAAS"
     gene            complement(27380..27946)
                     /gene="nthA"
                     /locus_tag="VFQ10_01070"
     CDS             complement(27380..27946)
                     /gene="nthA"
                     /locus_tag="VFQ10_01070"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HET9963958.1"
                     /translation="MSPYAARIRAIEALLLEKGVLTEPEVQDSISYMEARSPANGAKL
                     VARAWVNPDFKALLLSDGKAAASELGMAPEHPAEFIVVENTPELHNLIVCTLCSCYPR
                     AILGRPPDWYKSFEYRSRAVREPRAVMREFGFEPPEGVEIIVHDSTADVRYMVLPMRP
                     PDTEDLGEEELAELITRNSLIGVNVPRS"
     gene            complement(27982..28272)
                     /locus_tag="VFQ10_01075"
     CDS             complement(27982..28272)
                     /locus_tag="VFQ10_01075"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HET9963959.1"
                     /translation="MTFEPGDRVRVLPSEKPGHVRTPAYLKGKAGWIESLIGAFPNPE
                     EVAYGLSGLPERPLYKVGFRQTDLWDDYRGPAEDYLYADIYEHWLEPERGTT"
     gene            complement(28269..28568)
                     /locus_tag="VFQ10_01080"
     CDS             complement(28269..28568)
                     /locus_tag="VFQ10_01080"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963960.1"
                     /translation="MGGVHDMGGAPGGPIDRSQHEVEDWERLADAINGALGAKGLQST
                     DELRRSIESLEEYRELSYYERWVAATEKLLIEKDVLSSDEIDERAAEIERRWDGP"
     gene            complement(28579..29565)
                     /locus_tag="VFQ10_01085"
     CDS             complement(28579..29565)
                     /locus_tag="VFQ10_01085"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HET9963961.1"
                     /translation="MNAVEWFKHRSYDYRQFSDLEALGSRKRDLGLTVSAVLPCRNVA
                     DTVGGIVDEIHAVNCLTDDHPLVDQILAVDADSPDGTAEVAASKGAEVYSENELMSNH
                     GGAHGKGDAMWRALSVARGDLVMYIDADTKDFKPQLIYGILGPILEVPEIRFVKAAYR
                     RPFKSYETVEQDGGGRVTELSAKPLFNLFYPELTGFVQPLAGEFVADRELFSSIPFLT
                     GYAVETGIMIDVLKMVGLEAMAQVDLGTRQNRHQPLRDLSRMAYSVLRAVARRMRQEG
                     RLNQVRDPGMPDSLFQLSDYLHAVATPDGLKLQEYVEELVERPPIKEVMTVG"
     gene            29786..>30041
                     /locus_tag="VFQ10_01090"
     CDS             29786..>30041
                     /locus_tag="VFQ10_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET9963962.1"
                     /translation="MRIVLTNDDGIEAPGLLAARRALEKVGDVITVAPDQNRSGVGRS
                     ISFGRSLHVEEREMADGVTGYACTGTPVDCVRLVALGLMDF"
BASE COUNT         5824 a         9149 c         9726 g         5342 t
ORIGIN      
        1 ccctcgccgt agatccaacg tccctcaccc cacaggagta gccatgccta acacgaacgg
       61 ccacggtcta aagcgggccg tcctctgggc cgtcctctac gcccgcgtct ccacagacga
      121 gcaagcccgc tcaggctact cgctcgccca gcagctcgaa gccctgagag cctatgccgc
      181 ccgtgaaggc tacgaggtcc tcgaggaagt gcaagacccc ggacagagcg gggcgagcct
      241 tgagaggcca ggcatggacc gcgtaagaga cctcgtggcc ggtggcggcg tctccgtagt
      301 gctcgctcaa gacagggacc gcttcagccg ggaacccgcc tacacctacc ttttacgtcg
      361 ggagttcgag gaacatgact gcgagctcag gtccctaaac gacagagggg acgggagccc
      421 ggagggggat ctgacggacg gcatcctcga ccagctcgcc aagtacgagc gggcgaagat
      481 tgccgagcgt agtcgaaggg ggaaactcag gaaggctcgc gagggaaagt tgataccgtc
      541 acccaccccc gactacgggt tcgattacaa cgggacgcga gacaactacg tggtcgatga
      601 ggagaagatg caggtggtgc ggcgcatctt ccgcatgatc ggcgccgaag gcgatgcatt
      661 gaacggcgtc aagaagacat tcgaccgcga gggaattccc acgccgaggg gttctagata
      721 ctggggtaac actttcatac agcgggttgt caccgacgac gtgtacaagc cccacacttt
      781 cgaggaggtg aaagccctgg tttcgccaga ggtcgcctcc cgtctggatc ccgacaagag
      841 ctacggcgta tggtggttca accgtcgccg caggaccgag aagcaggttt ctgagatcgc
      901 gtccgacggc cgcaggcgct acgtgcggcg ttccacgaca acacagaagc ctaaagagga
      961 gtgggtcgcg atcccagtgc cggacgccgg gataccgcgc gagtgggtgg accgtgcccg
     1021 cgaggccgta agcgacaacg tgaagttcag ccgcgccggc ggacgcttct gggagctatc
     1081 cggcctcatg aagtgctccg aatgcggtcg cgggatgctc ggcaacagtc ctcccaacgc
     1141 ctcccgcaag aaggtctacc attattatcg ttgccgcacg cggcacatgg agggcacgga
     1201 agcctgctcg atgtggaaga atatccgtgc aggcgaagct gagcgtgccg tgtggtcgtt
     1261 tgtcaccgac ttactcttga atccggatgc cctgagggaa gggctcgacg agatgatgag
     1321 acgggaactc gctaccaacc agggtaaccc ggaggaagag gccgccgtct ggttcgaccg
     1381 tctggtggag gcggacagca gacgtgccaa cttccaggac atggccgccg ctggcctcat
     1441 caccttcgac gaactcggag ccaagttgga tgccctagag gaagcgcgca agaccgcccg
     1501 gagggaactc gccgccatcg agggtaggaa agagcttctg cgggccatcg agcgtgaccg
     1561 ggccgcactg ctggagggtt acgccggcct ggtgcccgag gcacttgacg acctagactc
     1621 cgaggagcgc caccgcatct acaacatgct gaggctgaga gtctcggctt tccctgacgg
     1681 gacgttagaa gtaggcggta ccctgagcga tgggcatctt gtttgtaaaa agtcgacgag
     1741 aaggttactt tctcgcggca catcaagagt aaggtcgagg aggtcgagcc ggggaaggcc
     1801 gtctgccaca tagacgtgga gagcatccac ctcaacggca acgggacgct gcacggtggg
     1861 gtctacgcct cgctcataga caacgcgatg gggctctccg tggccgcgct cgtcggcctg
     1921 aggacggcca ccatcggcct cgacgtccac ttcctcggtg ccgtgaggga gggtaggatc
     1981 acctgctccg ccggggtcgt ccaccgcaca cgccgcatcg ccaccgtcga ggccagagtc
     2041 cgcgatggcg atggaaacct cgtggccatg ggtacgggaa ccttccgcat cttcgaaaag
     2101 cgtggcgacc ccatcgtctg acagcacttc agctcttggt tttgctgagg gctgagaggc
     2161 tgatggctcg gtagccaccc tcccacgcgc atggctgtac ccacacagta atcggcgcgg
     2221 ccggggtttg accggtatca ttgcaggtca gatacacggg agagggaacg ctggtggcga
     2281 ggaggagata tgttccgtaa ggagagaccg tggctcgggg tctacgagaa ggggagagtg
     2341 gagcctgaga cgcagatctt cgagggctct ctatacgaac tgttttcggg tgcggtcgag
     2401 gagcaccatg ggaagactgc gctgagcttc tacgacacta ccttcgagtt cgggcggctg
     2461 caggcccttg tagagaagat ggcggcctcg ctcgcggcga gcggggtcga gaagggagat
     2521 cgggtcgcgc tcatgctccc gaactgcccg cagtatgtgg tctccttctt cgcgaccgtg
     2581 aggctcggtg ctatcgtcac tcagatcaac cccatgtacg tagagcgcga gatagagcat
     2641 attttgaatg actccggcgc cgaaaccatc gtcgtatatg ccgacgtgta cgagcgggtg
     2701 aaggccttgc tgcccgatac gaacctgaag accgtcgtag tcgtggactt cgtgggcgag
     2761 caggaggggc tgaacgccgg gcaccgctac ttcggagatt tccttgcgac agatgacgaa
     2821 ccagcccctg aggtggatat agatcctgcc ggggacgtgg cggcgttgca gtacacgggc
     2881 ggaacgacag gggtgtcgaa gggtgcgatg ctcacccacc gtaacctggt cgcaaacgtc
     2941 cagcagacta tcgacgtgtt cgtgcgcgat ccggcccagt tcacagggcg caagtgcgtc
     3001 ggcgcgctgc ctttcttcca catctacggc cttacttgcg tgatgctctt cggcatcagg
     3061 ctcggcatcg agcaggtact gctcccccgc ttcgaggtgc aggaggcgct ggcggtcttc
     3121 gagaaagacc ggccgacgat gttcgccggc gttcctacga tgtacatggc gctcctggcg
     3181 agcggggcgg acttgcgcaa gcaccacctg cacgacgtac agatcttcaa ctcaggtggc
     3241 tccgccctcc ctgtaaacct gaaacgttcc ttcgaagaga aggtcggcag aaccctcttc
     3301 gagggctacg ggctctccga agcctccccc gtgacacaca acaacccgcc gttcctgggg
     3361 cagggacgcg agggaagtat aggtatcccc attccttcga cagaggccag agtagtagac
     3421 gtggagacgg gggagaccga gatgccaatc ggcgagtcgg gagagctgat catcaagggc
     3481 ccgcaggtta tgaagggcta tctcaacatg cccgacgaga cggctgagac tctcaaaggc
     3541 ggctggctct acacgggcga cgttgcgaag atggacgaga gcggctactt ctacatcgtg
     3601 gatagaaaga aggacatgat cgtagcctca ggctacaatg tctacccgcg cgagatcgaa
     3661 gaggtcctct tcgaacaccc cgacgtggcc gaggccgtcg caatcggtgt cgccgacgag
     3721 taccgtggcg agagcgtcaa ggctttcgtc gtaaagcgat ccggcgcctc gacgacggag
     3781 gaggaggtac tggccttctg caaggagcgc ctcgccgcct ataagacccc taaggccgtc
     3841 gaattccgcg aagaactgcc caagagcgcc gtgggcaagc tattgcgccg cgtcctggta
     3901 gacgaggaac gcgccaagac agaggcaaca cctcggagtt aggaggagaa tgtcttgaac
     3961 gccctggatc tcttccgtct cgacggcagt acggctatcg ttaccggtgg cgggcgcggt
     4021 cttggccgct acatggcaga ggccctctct gatgccgggg ccggcgtcgt gctgtgctcg
     4081 cgcaagatag aacctctcga ggaagtaagg agcgagatcg aggctggagg cggcagggcg
     4141 ctggccgtgg agtgcgacgt cacagacccg gaggacgtgg aacgagtcgt gtcgcaggcc
     4201 gaggaggctt tcgggagtct cgatatagtc gtcaacaata gcggggctac gtggggagcg
     4261 ccgcccgcgg agatgcccct ggagcgcttc gatcaggtac tcagggtaaa cgtgcgaggg
     4321 acctttctca tgtcccaggc ggccgggcga cgcatgatcg agcgcggttc gggcggtacc
     4381 atcatcaata tctccagcgt ggcaggcttg gtcggcggcc accccgacta catgcagacc
     4441 gtaggctaca actcgtccaa aggcgccatc atctccatga cccgtgatct ggcgacctcg
     4501 tgggccccgc acggcatcac cgtcaacgcc atagccccag gctggttccc cacccgaatg
     4561 agcggcggcc tgatcgagaa gttcgaagag aggatgctcc agggcatccc tctacaccgt
     4621 ttcggcaacc ccgatgacct caaaggagtc gtgatcttcc tcgcctcacc ggccgccgcc
     4681 tacgtcacag gccagaccct ggtcgtagac ggcggcgcga cagcctggta gcaaagatac
     4741 ctcacttggg ctaataagct ccctcttgga ggtggggctg tggaactgtc agccactggc
     4801 gcgcagcgtg cgcagaacag cggcgagcac aggatcgatc tcggccaggt acgcccctac
     4861 gtgatctgat tcgtcaaatg agttgaactc cgcattgggg atctcattcg ccgcctgctg
     4921 cgcctggtcg tggaagtcga catctccggt cgctacgtag atgaggcatc tcagttccca
     4981 cgccccaaga tcttctgaga tatcgccctc agcgactcct gcgctccacg ccgcctcgat
     5041 ggctgcgggg tcgttgtcga gccaccgttc ccgcagggca tctggaaaac gggtaccggc
     5101 gaacgactcc agcatctcaa cgaagggctc catactccct tccgctcgcg actcggccag
     5161 agtctccgtg gcggcgtgag caagcggacc gtcggggtcg atggcataag gctgttggcc
     5221 gccgatcacg agcgagagca cgcgctcggg cgcatgttcg ccgatcccga agccgagcct
     5281 tcccccccac gaattgccga tgaagtgtgc gcgctcgatg ccgagttcat ccaacaccgc
     5341 caccgcatcc gctacgcgca acggcatcgc atatgcctcc acctcgtgag gcttgtcact
     5401 gcgtccgacc ccgcggtggg cgacgtaaac gaggcggaac tcgttcgtag gcagctcctg
     5461 cagtgcctgg gcgatgttcg actctcgcac cagatcaacc gagtcaatta tccctccgtg
     5521 caggacgacc ggggctccat cgccgccgtc gtcctcgaag tacacccggc ttccatccag
     5581 agcgttcgtc gcgtaaggca tgagcgctcc tcctctcgcc acctgattcc cattgtactc
     5641 ccctcgctct gagccgttct acggtgctcg ccctcgaacg gactaccttt gagtgttgtt
     5701 cggacgttgt tcgaagggcg gcgttgcgga cttcctgaga attcgccttc cgaaccatct
     5761 gaaaatgtaa cgtttgtgat ccgagtttgc gcggcaagcg tatacgcatc gccggacgaa
     5821 agaggctggt tgtgtggagg aagggagagg atatggcgac gatagacggt tggctcgagg
     5881 gcttcttgca cgggcctgcg gccctctggg tcatactcct ggtaagcctg gcgctgggtt
     5941 tgcgccacgc ctccgacccg gatcacctgg cggcggttac caccctcatc gcctccgaaa
     6001 gtggttacgg ggtgagaaag gccggctcga tgggtctttc ctgggggatt gggcacggca
     6061 cgtccctggt tctcatcggc cttccgctcg tgttgttggg ccgttacctg ccggagccgg
     6121 tgcagcaggt tgcggaggct ttgatcggtg tgatcataat catcctcgcc gcacggttac
     6181 tcgttcgctg gcggcgcggg cacttccacg cccacgtcca cagccacagc ggcgaacctg
     6241 ttcaccgcca cgtccactcc cacgaatctg actcctcgca cgggcacgcc catcacgtct
     6301 tccaccgcac accgctctct tcctatggaa tcgggctggt gcacgggata ggcgggtcgg
     6361 gcgggctgac gctgctgtta ctctcgacca tccccggccc ggggcaggcg acagttgcac
     6421 tcctgatctt cgccggcggg acagcggtct cgatggcgct cctttcgacc gcattcgggc
     6481 tcgctatagc cagcggtccc atcggcagga acttcgagcg cttgaccccg gcgctgggcg
     6541 cctcgagtct ggccttcgga acgtggtaca tgctcggcgc cctcggggtc gtggcgtatc
     6601 cgctctaggg agttaacaga tggaaagaga atccaacaac aaagggtggg tgcagagaga
     6661 gtgcccccac ccgcgtgggc gcggaccgcc tggagcacag ggttttcctg agttcgcaaa
     6721 ccgtatccgc gctatgggac cgcggttacc ttaacacata gagaggagga agcgtgcaac
     6781 cactgacgct acgagcgggc aagcggcaac tggtacttat cctgtttgcg ctggctatct
     6841 cggctatggc gctcggtgtc gttggtatct cgaagccggc ggacgcttca tcgcacagag
     6901 aagcgccgtt cataagcggt gatccgcagg ccgatgccac cgacctctac gcgttccgca
     6961 gccccgacag gccgaacagc gtcacgctca tcgcaaacta cataccggcg gaggtggcgg
     7021 cggcagggcc gaacttctac aggttcggcg gagacgtcct ctacgagatc aacgtcgaca
     7081 acgacgcaga tgccagagac gacctctcct tccagttccg tttccagaat cgcaccaaca
     7141 acggcaacac tttcctgtac aacacaggcc cgatcacctc gctgaatgac cagaacctca
     7201 accaggagca gttctattcc gtgtcgatgc tcaagggcat ggatcacgag gaggcactcc
     7261 gcgaaggcaa ggtcatcgcg aagaacctgc aggtcgcccc ggctaacgta ggtcctgttt
     7321 cctatcctga gggctaccgc aaagtggcca atcaggccat ctacgatatc ggggacggcg
     7381 tcaaggtctt cgccgggccg cgtgacgacc cgttcttcgc cgacctcgga ggtatcttcg
     7441 acctcctgca agggatcgag ggcgaggact acctcgcgga tctgaacgtc cataccgtcg
     7501 ccattcaggt gcccatcgat aagctgacga cgggcgacag caacatcatt ggtgtcagga
     7561 caacatccta ccgccagtcc atgagtgtcc tccgtcctat aggacagccg ggttcgcaga
     7621 ataacaaccc gagctcctcc tcaggaccct gggtacagct ctcgcgtctg gatatgccgc
     7681 tggtgaacga gctggtcatt ccgctgaagg acaagaaccg ctggaacggc tctgagccgc
     7741 gcttcgacag ccagtttgcg aagtacgtgc tcgatccgga gccggcccgt ctcatagaga
     7801 gcatcctcgg cgtcgacgtg cccaatccac ctcgcaacga tctctcaacg atcttcctga
     7861 ccggtattcc ggatctgaac aagccggctg gcgtaaaacc ttcatcccag ctcaggctga
     7921 acacggccat acagccgtca gataacccgg aacggcttgg ggttctgcgg ggtgacaacg
     7981 cgggcttccc gaacggacgc aggccggccg atgacttagt cgacatcgag cttcaggctc
     8041 tcgcaggagg cacgccgttg acccccggct tcaacaaggc gccgaacaac cagctcggcg
     8101 acggtgtcga tgccaacgac gtgccactcc gtaacagctt cccgtacctg gctgacccgc
     8161 aggactatac ggagacggag taacgagaga gcactggttt gtggtgggga ggacaggcct
     8221 ccccacccgg cctcgaacat atttcacagg aggaaggtag atgacggtaa tccggagaag
     8281 ccggcttcta tgggccttga tcccggtgct cgctgccctc gccacgctgg cgacactggc
     8341 tctcttgcga ggcgaacgtt ctgcggatca gcgcgtcgag ctaccctttg atttccgcca
     8401 gaccgctggc acttcgacgg atagtctcat cggctcactc caggaaaaga tcagggataa
     8461 cccgaaggac ttcgactcgc acatcaacct ggccaatgcc tatctccaga aagtgcggga
     8521 gacgggcgac ccgaccctgt acaccaggac agaagacctg cttggtaatg ccagaaagct
     8581 agaagctcac tctccagagt tgttcgccac gcgggggtcc ctcgacctcg cgcgccacga
     8641 cttcgcggcg gcgctgcaga acggaacgcg agccctggcg ctcgaccctg agaacgcccg
     8701 ctaccacggc atcgttggcg acgcccagat cgagcaggga atgtacgagg aggcgatcaa
     8761 ctcctaccag aagatggtcg accgcaggcc cgacttcgat tctttctccc gcgtggccta
     8821 cgcccgcgag ctctacggcg acccggaagg cgccatcgaa gcgatggagt tcgcgctgca
     8881 agccggaagc ggtacacccg aaaacgtggc ttgggcacac gtgcaactgg gcaacctctg
     8941 gttcacttca ggcaaactgg aggaggcgca gaaggcatac accctctcga ccagaacggt
     9001 caacgcttac gcgccagcac tagccggcca ggcgaagatc gcagcggcga ggggggatct
     9061 cgaacaggct gccacactct acaagcaggc attcaaccgc atgcccctcc cggagtacgc
     9121 aatagccctg ggcgacgtct acgccgcgat gggcgaaaga gtaaaggctg aagagcaata
     9181 tgaccttgtc aggaacatgg acaaattgtt acgagcaaac ggggtcgaca ccgatctgga
     9241 gatcgccctc ttcaacgccg accacgacat tgacctccgg atgtcgctcg aaaaggcgcg
     9301 cgcagcctac gaagcgcggc caagcatcca cgccgcggat accctggcct ggacgctata
     9361 caagacaggg aactacaagg aggcgcgaga gtattcttca gaagccctga agctggaaac
     9421 ccgcgacccc ctgaagctct tccacgccgg catgatctcg aaggcgctcg gggagaaaga
     9481 gcaggccagc acgtacctgc aacaggccgt tgacctgaac ccgcacttct ccctgctcta
     9541 cgctgacgag gctgcttaca ccttgaagac cctgaaggcc ggaggggaac actgatggtc
     9601 cggtggggtt ggcctttggc gtgtctcgcg ttcgccgtgt tcgcttcgct gatcccgcgg
     9661 gaagcgcagg cgcatcctct gggcaatttt acaatcaacc actacagcag actcgagttc
     9721 gctgaggaga ccgcgcgcat cacctacgtc ctcgacttcg ccgagatccc aaccctccag
     9781 cagaaagcac gtctggacgc caatggcgac ggggagctct cgggcaaaga gtcaaacgcg
     9841 tatctcgacg ccgagatgcc atctctcgtg gaaggtatac ggcttcgcgc gggacacgag
     9901 gtcttgccgc tggaggtgct ggatcgctcg gcggagtacc ggtccggcca gggagggctt
     9961 ccgaccctac gagtggagac tcatctcctg gccgaccttc ccaaagattg gagaggtgag
    10021 gcccattaca ccgaccagac ctacgcgaac cgtctcggat ggcgcgagat cgtggtcagg
    10081 ggcggacccg aaatggctat caggaactct acggtacagg cgaatagtgt gtcggacgaa
    10141 cttcgctcct atccgcgaga catgctctcc agcccgcccg atgtccgcga ggcgtccttc
    10201 acgctcgctc ctggcgacgg gtcggtccat aacgacgcgg cgggccggac ggcggagcac
    10261 ggcggaacga gcttcggcgg cgtgacaggg cggatcgacg cgctggtctc cgtggagcgt
    10321 ctatcggcgg cggtcgtagc gctggctatg ttggccgcgt tcttctgggg agcggcgcac
    10381 gccctgaccc caggtcacgg caaggcagtc gtggcggcgt acctgatcgg ggcccgtgga
    10441 accgccaggc acgcgggcat cctcgggctg acggtgacgc tgacccacac agccggagtc
    10501 tttgtgctcg gagccgtgac cctctatctg tcacgctaca tcctgcccga agtcatctat
    10561 ccctggctga gcgtagcctc cggactgctc gtcgtcctga tcgggctcgc cctcctttac
    10621 cgccatctgg acaaaatgtt ccacgacagg aaagccgagc acacacaccc gcatgatggc
    10681 gcacactccc ccggtggttc acgctccgac gtccatcata cccattccca cggcgggcac
    10741 agtcacttgc ccgtggatgg ctcgaagata acctggcgcg ggttggtggc gctcggcgtc
    10801 agcggtgggc tcgtgccgtg tccggcggcg ctcgttctgc ttctcggtgc gatctcgctc
    10861 gacaggctgg ggttcgggat ggtgctcgtc ctcgcgttca gtgcggggtt ggcgataatt
    10921 ctgaccggga tcggactgct tatgatctac gccagaaagc tgttcgagcg gttcactttc
    10981 gaggcgagag tgccgcgcct gttgccggtc gtgagcgcct cgatcatcac gctggccggg
    11041 gtgggtgtag cgctcggagc cgtccaacaa accgggatcg tttagccgat gaatcccggc
    11101 ttgttacact gggtctgagg cgccgggtcc tggcgtattc ggccctggcg ctggggcgat
    11161 tacgggacgt cgcgggaagg aaagtagcgt gaagcccgaa cgagcgggcg aaccgggcgt
    11221 ggacgaaagg ttggctcgat atccgttgct ggatgctctg tgggagcgcc gctcgacctc
    11281 cagaaccgta gggccgaatt ggagggttgc ttgatgaaga cgggaacaag tgtcggcatc
    11341 gcctgtgctg tcggcgtggt ggggggtgtc atgatcgggt ctcgcgccgt caagatctac
    11401 gactggcgca ccgaatggac cattcccgcg ccgatgcccg tcgtctacga ggccatgacc
    11461 tcgcgccagg cagtacgtga gtggtggcct gacatggaac tcgccgagga caacggagat
    11521 gacggcctcc aggttggcag caccgtctct ttccgggtac actaggcccc cgaggtcgca
    11581 cgcctcgctc cgcccttccg catccactgc ctatacactg acgtagaacc ggaaaggagg
    11641 ctgagggaga cggtgagcgg agacctgagc ggtgtactag agactctgtt ccacgagcag
    11701 cacgacggca cctccataac attcaactgg tacgtgcgcc tgaccaaccc ggctctcaac
    11761 ctcctggggt acgccgccga acgcgtttac cgccatagcc acgacagcgt gatgacctcg
    11821 ggcgagaaag ggctctcgga gtactgcaag cgacgggcag cctccgggcg cacgtaggca
    11881 gtggatccca aggcaacttc aaccacagaa agggagtacc gaatgacaac ccagccagtc
    11941 gagaaggaga ccgctccgct aagatctaca tctttcgaga ggttcgctgg gatgtgtggg
    12001 gtccttgccg gtgtcgccgg ttttctgtac gcggtctctt tcgtctcgta gaaaggcgag
    12061 ccggtgatgg gggcggtgct cgccctgccg gctggggtat agatggtggc ccggctcgga
    12121 atggcacctt caggagcgcg ggtttgactt agcgtagaat aacgtccctg caaagccacg
    12181 agcggaggat cttctttgat gccgaacgtg accatcatcg gtgccgggct cgccgggagc
    12241 gaggcggcct ggcaggccgc agaactcggc tgctcggtcg agttgtggga gatgcggcct
    12301 cgaagagaga ctcctgccca ccgcacggag cacttcgccg agctcgtgtg ctcgaactcg
    12361 ctcggaaacc tcgccctgga gacggcctct gggctgctca aggaggagct aaggcgtctt
    12421 gggtcggtga tcctacgctg cgccgacgcc aacacggttc cggcgggcgg cgccctcggg
    12481 gtcgcccgcg aagattttcc acgtgccgtc accgagacga tccacgccca cccgaacata
    12541 gaagtgatcc gtaaagaggt cacggacatc cctcagggtc cgaccgtggt agcgacaggc
    12601 cccctgacct cggatgcact agcaaagaag atagaggacc tctccgggga gacgctctac
    12661 ttctacgacg cggcctctcc catcatccac cgcgactctc tggacgactc catcatctac
    12721 cgcgcctccc gctacggcaa gggcgaggcg gcatacttga actgcccgat gaccgaagag
    12781 cgatactacg ccttcgtcga ggatctggcg gccgccgagc taagccccat aaaggacttc
    12841 gaggaggacc tgtacttcga gggctgtctc cctgtcgaga ccatcgcccg ccgcggaaag
    12901 gacaccctcc gcttcggacc tatgaagccc gttggccttc ccgacccgcg cacgggagaa
    12961 atgccctacg ccgtcgtgca gctgaggcag gacgacgccg agggctgcct gtataacatc
    13021 gtcggcttcc agacgcgctt gaagtggggc gagcagaggc gcgtattccg gactatcccg
    13081 ggcctccaat acgccgactt cgcccgcatg ggcgtcatgc accgcaacac gtacctgccc
    13141 tccaatcaca tgctagacgc cacgatgcgg ataagaaagg cgcttggcga agcccccctc
    13201 ttcttcgcgg gccagctcac cggtgtcgag ggctacgtcg agagcacggc gatgggccac
    13261 atagctggta ccaatgccgc ccggctggca cgtggcgagg agcccataac catgccccgc
    13321 ggcaccatga tgggtgcgct cgcccactac gtcaccacca aagaaggcac cctgcagccc
    13381 ataaactcca actggggcct ggtccccacc gtgccgaaaa gagagaacgg caggcgcctg
    13441 agcaaatccg aacgcaggca acgccaggct tggatggcgc tttcgaggct cgatgagttt
    13501 atgcgggagg gcataaagac cgccgtttga acggcgggct ctgcgcgcca ccgaactgcg
    13561 gctagggaag gcgctccccg gtgtgccagt gatgctaagc tgccagggga acgacggtga
    13621 aggccattac cacctacctg tccgccgggc acataggggc gttcatcaag ctcttccccg
    13681 tggtctcctg gctcgtgaag gaacgccgcg aactcgccgt aattcgacag atacccaccc
    13741 atgagaggct gggtatcgcg ttcgccaaga ccaacgtcgc gctgtgcaaa gcggtgaaca
    13801 agaacctggc aagtatcagg gagcgtggtc tattcgatgc tttacgccgc aagtggctcc
    13861 ctgagtcgga caaggattag gcgggtatgg cgatcaggct cctccgcatc ttcaccggcg
    13921 acgacgacca gtctcatttc tctctcgatc tcccgtacgt gctgcggggc gaacgcttcg
    13981 ctatgctgta gtcgtagctg gcgaccatgc agggaggcgg cgatgtcgga gacgatgtcg
    14041 gataaggaga taccagagac ctgggttgga cagcgcgtca gggtgggcca cgctcacagc
    14101 gcgtccaccc acacagacgg gttgttgctt ggggttggcg accgaggcat tgttgtcgag
    14161 ggcggcgagc tggtccgctt ctacccctgg acctccgttg ggagtatcga gaagacccac
    14221 gaggagggtg gcgagaatcc tggcgtacag gcgggccggg cctggtagca accgcttccc
    14281 agccatagac ggactgcgtg aggaagctgc gggcacgtgt tctacccggt tgggggtaga
    14341 ggtttgtggc ccacgaagac ctcgacgcgt tcaccgccgc tcgaagggtc ttcgacgact
    14401 tctggctcga acccgacgtc ttcgagcagt cgtagccagg tcgcgcgtgg aaagattcca
    14461 acgacctgac ggtccacgac ggcgcgcagt tcgtcgccct ctttcaaagc caggatgaac
    14521 tcgtagttga ctttggtgtc gttcggattg gcgtcccagg tccactccag gtacctgacg
    14581 ccccgatcgc ctctgtcaac cccctcgtgc gtggtgccgg gccggaaggt ctcggccacc
    14641 cagtcgggta cgaacaccgc gactccaccg ggtttgcagt gctcgtaggc cgtgagcagg
    14701 gccgcgcgta gatcctcctc gctgctcatg tacatgatgg cgtcgtgaat caggatgccg
    14761 tcgaacgtcc tgtccaggcg caacgttcgc atgtcgccct cgatgtgttc gagttcgggg
    14821 ttttgcttcc tgctctcagc gagcatctcc tccgagatat cggtcaacgt cagggcgaaa
    14881 tcctctttga ggaaccaggc gacgttgcca ccgccgcagc ccagctcgag cacggttccg
    14941 gcgttctcgg cccggaagag cctgccgaag aaagcggcct cgtccgcgta gtcttcggtc
    15001 ggcgagatca actgccacca gccggccagc tcggcgtaca atttcggatg cccggtactc
    15061 actctacaac gttaccactt cgccgccgtg acctggggac gaggtttcta cagcttcgct
    15121 gttacctccg tacgtaacgc cgcgtcgaga atggtctcga cgagcatctc gaaggtggtg
    15181 cctgcggtct cggccgcgag ggggaaggtc gatgtctcgg taaagccggg gatggtcttc
    15241 acgtcgagga cccagggggt gccttcggca aagatcgtat ccaccctggc gaaaccggaa
    15301 cagccaagga tcttgtagat ggtgagcgcc gtctcctcta cgcgggaggc ggcctcggac
    15361 ggtatctccg ccggtatggc gaagtccgtc gccccggggg tgtatttagc ctcgaagttg
    15421 taggcgcctt cgcgaggtct tatctcgacc agtcctagcg cctttggctc ctcgcccgcc
    15481 ggttcgatga tggggataga gatctccgtc ccctccaccc agcgctcgag caggatcttg
    15541 gcgtcgtagc cgagcgcttc gtagacggcg tcgaacagtc cttcagctcc ctcgacccgg
    15601 gatagcccga agctcgaccc ctccctggca ggcttgacga cgagcgggta acccagttcc
    15661 tcggcggcga cgtcgagcgc cgccgaggcc cccatctcgt tcatcgccct tctcaggaag
    15721 gtgcggaaag ggggggtctg tatgccggcg gcgcggagcg cacgtttggc gtagtccttg
    15781 tccatgcagc ggatggaaga gagcggcccg ctccctgtat acgggatgtt cagggtctcc
    15841 agcagcgcct gcacagtccc gtcctcgccc ccggggccgt gaaggcagat gaaggcggcg
    15901 tcgggtgaga tctccatcag agtccccgtg gtcgtgtcct ctatgtcgag cgggtaaacg
    15961 tcgtggccga gtcgttcgag tgcgtgctgg acgcgccggc cggtaatgaa ggagacgtcg
    16021 cgctccatgg aatgaccacc gcgcaggacg gccacgcgag ccacagtacc ctaaccttcc
    16081 gtgcctgaac tgaagcccac cgcgcgggtg ccgtgtattg cgccttcttt tcggtgcgag
    16141 ccacgttcca ggtcgctcct gagctccatg cgctcccgga tcacttcgga gagcagttct
    16201 atgccccgcc ggatcctctt gggttccacg aacgagaagt tgagccgcat gttgttcttg
    16261 cctgtccctg gagcgccccc gtagaacccc tcgcctggca cgtaggctac gttcctggcg
    16321 atggcgcggg ggagcatcgc ggttgcgtcc aggtacgagg ggagggtggc ccagacgaag
    16381 agcccaccct cggggtgggt ccagtagacc tctttgggca tgaactcggc cagagagtcg
    16441 agcatcgcgt cgcggcgctc tttgtacatg gaggtcaggc gccctacgta ggctctccag
    16501 tccgcgttct ggaaataaga ggagatcatc atctgagata ggttcgagga gcagaggtcg
    16561 gcgccctgct tgccgacgtt gatcttgtgc agtatcccag gctgggcgtg cacccacccg
    16621 agtctgacgc cgggggcgaa gatcttggag aacgtcccga gatagacgac cctgtcgagg
    16681 tctccctctt cctgctccag cgccgcgagg gaggggagcg gctcgccttc gaagcggagc
    16741 atcccgtagg ggttgtcctc cacgatcacg agatcgaact cgcgcgccat ctccaagagc
    16801 tccctgcgcc gctcggcgac catcgtaacc ccagcagggt tctggaagtt gggaacggtg
    16861 tagaggaact ttacgtggag cccgcctttg cgggcattct tgagggtctc gcgggcagcg
    16921 acggggataa tgccggccct atccatcggg gcgtgggcta tgcgtggcct gtaggcggcg
    16981 aacgcgttga gcgccccagc gtaggtaggc ccctcgcaca ggacagcgtc gccctcgtcg
    17041 aggaagacct tggcgatgag gtcgagcccc tgctgggctc ctgtcgtgac gaagacgtcc
    17101 tcctgcctcg ccggcgtgcc ctccgcgccc atgacctcga ctatgacgtc ttttatggct
    17161 tcgaggcccg ccgtcggccc gtattgcagg gcctgggcag cgtccgaagc gatgttgcgg
    17221 gagatctcac ggaacgcctc ctcgccgaag gcctttgtgt ctggaaagcc gccagccagc
    17281 gagatgatgc ccggccgcga gagcgtggcc atcaggtcgc gggtcgcggc cgacttcatg
    17341 cccttgaccc ggttggcata caggaaatcg aatcggtcga ggtctacgag cggcaagggc
    17401 tatctccccg cgctttggtc gagaacctca tcttggctct ctgcgtctcc acactcacct
    17461 catgcatgga cgcgactgta tcataaagaa tcgcccttat gtagactctt acgctgtgaa
    17521 agctatcttc gtaaccacca acgaacacaa gcgtcgtgaa gtacaggaga tcctcggctt
    17581 cgagctggag cgtgcggatc tggacctgcc cgagatacag gccatcgacc ctgctgaggt
    17641 cgccgcagac aaggcccgcg ccgcccgcga ggttctgggt agaccggggc taccggtcct
    17701 ggtcgaggat tcagggctca tggtcgatgc gtgggatggg tttcccgggg cgctcacaaa
    17761 atggctcatg cagagcatcg gcagcgaggg actgctcagg atgttgccgc cgggcgagga
    17821 ccgttccgcc agggccgttt gcgtcgtcgc gctagcgcag gctgacggca acgtgctcac
    17881 cttcaggggt gaggtgccag gaactctggc gcagaggcca cggggtaccg gtgggttcgg
    17941 ctacgatccc gtgttcatac ccgagtggtc ttcgatgacc tacgccgaga tgggcgaagg
    18001 caagaacgaa gactcccaca gggcgcgggc cttcagggct gtgcgggagt ggttgggaga
    18061 agcttggagc ggggtatagg agcgtatgga aatacgtacg aaacacgcgg ttttggtatt
    18121 ctgtctttag gtttgtacct ctgagaggga aggggccgtt aatgtcggca gataacgagc
    18181 actcagaaga cggcggcgta gagacccgcg aaggcggtgg tgccatagcg accagctcgg
    18241 agcgtctcgt agacctgtac cgtcagatgg ccctgatccg ggccttcgag gaggcgtgcc
    18301 agaggggctt caggcagggt aagatcgggg gctacctcca cgtctacata gggcaggagg
    18361 ccgtcgccac tggctttttg gaggccttca aggaaggcga caaggtcatc acagcctacc
    18421 gcgaccacgc gcacgcacta ctcttgggca gtgatcccaa ggagatcatg gccgagctct
    18481 acggcaagga gacaggtctc gtaaagggca agggcggctc catgcacctc ttcgacgtcg
    18541 agcgtggcct gatgggcggc tacggcatcg tcggcgggca tatacccctt ggggttggca
    18601 tcgcctacgc catgcgttac caggagaccg atcacatcac ccagctctat ctgggtgacg
    18661 gtgcgatgag ccagggcgcc ttccacgagg cagccaacct cgctgggctc tggggaaggg
    18721 acggccagtg cccttgcctg ttcatcgtcg agaacaacaa gtacgggatg ggcaccagcg
    18781 tcgagcgcac cacagccatg accgacctgg ccgccaagtt cgacgcctac ggcatcgaga
    18841 acgagaaggt ggacggcatg gatgtcgaga ccgttatcga ggtcgccgag agggtggccg
    18901 agcaggtaag ggagacgggc aaaccgtacg cggtcgaggc gctcacctac aggacggccc
    18961 cccacggcgc ggccgacttc ttcgagaagt accgcaccaa ggaagagatc gaagagtggc
    19021 gcgagcgcga ccctatcgcg atggtcgagc ataagctcat cgagaacggc gcgctcgacg
    19081 aggagaagct cgaggagatc aggggcgagg ccaaggagcg ggtacaggag gccgtcaagt
    19141 tcgctgagga gagcgaggag ccgccggagg aggagctcta caccgacgtc tactcaggca
    19201 aggacgagta tatgggggag gagtaggccg tggcggagac gaagacctac cgcgaggccc
    19261 tgagggaggc gatggtccac gagctcgaca gggacgagaa cgtcgtcctc atgggcgagg
    19321 acatcggtgt ctacggtggc acccacctta taacggacgg cctctacgac gagtacgggc
    19381 caaggcggat aatggacgtg ccgatctcag agaacgggtt tacaggcgcg gcggtcggca
    19441 tggcgatgat ggggatgcgc cctgtggtcg agatgatgac ctggaacttc tcgtttcttg
    19501 cggcggacca gatcatccag aactccgcca aagtgcgcta tttctcgggc gggcaggtag
    19561 aggtgccgct cgtgatccgg ggccccaacg gcggcggcgt ccaactctcg gcccaacaca
    19621 cgcactccct ggagagcttc tacggccact tccctggtct caaggtcgtt gcgccagtga
    19681 ccccgaacga cgtcaagggc atgatgctga cggcgatccg ggaccccaac cccgtgatct
    19741 tcctggaagc cggcgccctg tacggtacga agggcgaggt cgaagagggc gataacgcag
    19801 tacctttcgg cgaggcccgc atcgcccgcg agggctcgga cgtgacgctc atcgcatacg
    19861 gccgccaggt caacctgtgc ctgagggccg cggacacact cgcggaggag ggcttcgaag
    19921 ccgaggtaat agacctgcgc tctttgaggc ccttcgacga ggacaccatc gtcgagtcca
    19981 tagagaagac gcacagggcc gtggccgtcc aggagcagtg gcggtggttc ggggtggcga
    20041 gcgaggtggc ggcgatcatc caggagcggg ccttcgactc gctcgatgcg cccgtagagc
    20101 gggtgagcgg cgccgaagtc cccggaccgt acgcgcgcaa tctcgagttg atggcattcc
    20161 ccagcgagaa gcaagttacc aacgcggccc gtcgggttct gtacgaggag gagaagtaag
    20221 tgcctgaagt cattatgccc aagatgggcg acgcgatgga ggagggcacc ctcctcaagt
    20281 ggctcaagag cgagggcgaa gaggtctccg agggggaccc catagcagag atcgaaaccg
    20341 acaaagtcac gatggagctc gaggccgagg acgaagggac gctcgcacag ctgatcgcgg
    20401 acgagggcca ggaggtgccc gtcggcgatg cgatagcgtt tatccagggc gagggcgagg
    20461 aggtccccga gcgcagcgag gggggcgggg aagcggcaga ggaggccgaa gagggcggag
    20521 aggaagacgg tggcggagca caggccacag ccaccgagac cgaagcgcct gaggaaggcg
    20581 agactgagga ggccgacgga cgggcagacg gcagggccga cgggcacttc agggcctctc
    20641 ccatcgtccg caggctggcc gaggagaacg acctcgatct ctcgaagatt gacggctccg
    20701 gccctgccgg ccgcatcgta gagcgcgacg tgcgggccgc gatggagagc gggaccgccc
    20761 agaggaccga agacggggca gcgggcggcg tcgctgcgcc ggagcagccg cagcaggccg
    20821 agatgcagga cttccagccg gcccgcctac ctgagccgac cgaggagcct gggacacgtc
    20881 tcgtcgagcc tacgcggatg atgcaagtca tcggcgagcg gatgaccgag gccaagcagc
    20941 acataccgca cttctacgcc accgtagagg tcaggatgga cgcggcgatg gccctgcgta
    21001 agcagctcaa cgcacagctc gaagatgagg gcatcaagct ctccgtcaac gacttcgtga
    21061 tgaaggcttg tgcggtcgcc ctcaggagct acccgaacct caacgccctc tacaccaccc
    21121 gtggagtcga gttgcacgag aaggtagaca tggccatggc tgtggccctg gatcaggggc
    21181 tcataacccc cgtcataagg gacatcggca gcaagggcct cgctgccatc tccagggagg
    21241 ccaaggacct cgccatgagg gcgcgtgaag gcaagctcca gcccgacgag taccaggggg
    21301 gcaccattac cgtctccaac atgggcatgt tcggcatcga gagcttcacc gcgatcatca
    21361 accctcccca ggccgccatc gtcgccgtct ccagcatcac gaaacgcccc gactacgacg
    21421 agaacggcga gatcgtcccg gctagcatga tgaagctgac cctctcggca gaccaccgca
    21481 tcgcaaacgg ccgcgacggc gccctctaca tggccgaagt caagcgggtg ctcgaaaacc
    21541 ccgtgatgtt gatggtgtag gtgcgcggct gtcggggcgt gcgactccgt ctgcgaggta
    21601 gccgttggcg accatcgaga tgaattggta gacagcgtcc tcgcgggggg agaggggacc
    21661 ccggcggtag ccgcgcgagg agacgcccct ctgcacggcc tcgcatgcgg tccagtcctg
    21721 gcggttggtc acgtcccaga agtcgctggc gtatgagggg tcgaagtcct cctgctcgac
    21781 cgcctcgggt gagaagagcc actcgcactc gacgtgggtg tggtccggag ccaggggcgt
    21841 gagtcggtgg gtcatcacct agtcggggtg aagtcgaaga agtgctcctg ctcccagcga
    21901 atcacggacc cggtggtgta cgcctcggcc gggagtgtgc tgctgcggcc gaaagtgtct
    21961 agcgacgggg cgagaagctc gcaggatata ggcgtcacgt aatcctccct ccgtgcggga
    22021 cccgggtgga cggcgcttgc tgctagaatg ccgcaattgt acaggaaaga gtgtggagtc
    22081 tgggagggag cactttggcc tcagacggtt tgcgttacga cgctgtggtg gtcggcgggg
    22141 gccacaacgg gctcgtgtgc gcggcgtacc tcgcgcgcgc cggcatgaag acggtcgtgc
    22201 tggagcggcg ccatctggtc gggggggcag cggtgacgga ggaggtgtgg ccgggcttcg
    22261 gggtctccac cgcctcctac gtggttagtc ttatgtcggc gagggtcgta cgagagctag
    22321 atctctccag gtacggatac cacgtctacc ccctcgaccc tgcctacttt gcccccttcc
    22381 ctgacggctc gggcttcttg ctgtgggacg acccggggct ggcggccgag gagatcggga
    22441 agatcaacgt cagggatggc caggcgtacc tggaatacaa ccgcgaactt ggcgagctcg
    22501 ccgatctcgt acgacccctt ctctacagga ggccgccaga tcccggcatc cgttctctcg
    22561 ccgatgtggt cgaggcgctc tcgctcgggc tctacgcctt cggaaaccgg aggagcatct
    22621 cgcggctcgt tgacctgatg acgatgagct gcgccgactt tctcgaccgt ttcttcgtgg
    22681 acgagcgggt aatgggcgcg ctcgctcccg ggggcgtcat cggtatgtgg ggtggtccga
    22741 tgagcccagg ctccgcctac gtgctcttgc accacaggat gggtgagatc gacggcgtga
    22801 ccggcggctg gggattcgtg cgcggcggga tggggggcgt ctccgaggcg atagccgcct
    22861 cggcgcgggc cgttgggacg gagatccgca ccaacgccga ggtcgccgcc atagacgtca
    22921 aaggaggccg ggctacgggc gtcaccctgg ctgatggtgc ccgcatagag gccgggacgg
    22981 tcgtctccgg cgcccacccc cggacaacgc tcctctcgct tctcggcgca gagcacctcc
    23041 cggatgagct tgtcagggag ctcgagacct tcaggaaccg gagcccttcg gccaaggtaa
    23101 acctcgcgct ctcggagctt cctgacttca cggcgatgcc gggcaaagag ctcggaccgc
    23161 agcaccccga gaccatgatc agcccctccc tcgagtacct ggagaaggct tgggacgacg
    23221 ccaagtacgg gcgctcctcg gagggaccga tgatagacgc ggtgatcccc acgaccaagg
    23281 acccgaccat cgcccccgag ggtcgtcaca taatgacctg cttcgtgcag tacgccccat
    23341 acgaacccag agagggtaga tgggacggtg gcgccaggga ggccctgggc gataaggtcg
    23401 tgaacacggt caccgagtac gccccgaact tcaaggactt ggtgatcgaa cgccaggtcc
    23461 taacgcccta cgacctcgaa cagcgcttcg gccttatcgg gggcaacatc ttccaggggg
    23521 agatgtcgct cgatcagctt ttctctttcc gcccagcccc ggggctcgcc ggatacagga
    23581 cgcccgtaca cggcctctac ctctgcggct ccggcaccca ccccggcggc ggggtcatgg
    23641 gcatccctgg cctcaacgcg tcgaaggtgg taatcaaaga ccacaagagc gcggagcgaa
    23701 agcggcggct caaggagcgc ctggcctcgt attcgcgccg ttgaacggct atggttcaga
    23761 catctcgaag gggtatcgag ggaacggagt aaagagcatg aactagaaga ggaacatggg
    23821 aaggctggcg tcgctggccg tgaaacgcca ctacattatt ttctcttccc gaccttcttt
    23881 tcctttctgt tcttggcacg gccgctcttc ttccggttct gcgggatctc tcgtttctcc
    23941 tgttcttgct tcgcgacggt ggactccttt tcggcctcta tgggagattt gactccttct
    24001 gtgtccacgg gcttgtgcgg gaggacgttg tagatcacat aattctgggt gaaggtgatg
    24061 aggttggagg tcacccagta gacgaagagt cctgagggaa acctggcgag gaagaagcca
    24121 aagattatgg ggaggaacct cattagccgt ttctgctggg cggcggtgtt ccttatgctc
    24181 agctcctggg aggccatcat ggtgaggacg tagaggacgg ggaggatgaa aaggtggtcg
    24241 taaaccgtca ggtccttgaa ccagaacaga ccgccggttc tgaagctttc gagcttgtcg
    24301 aagtgcctga tcgtgtagta caggacgagg aagatgggta gctgaataaa gatgggcagg
    24361 catcctccga gtgggttgac ctgccgctcc tggtagagct tcgctatctc ctcccgctgc
    24421 ttctggacgt cgtctttgta ctcggcccgg atcctgtcca tgtcaggttt cagatcctgc
    24481 atcctgcgca tgctcttgac ctggcggtag gtgatcggga agagcagcgt acgcaccacg
    24541 atggtgagca tgacaatggc cagccaccac ggcgcgcccc aaccgtggaa ggtcaagagc
    24601 gttcctccca taaggttgaa tagcgggtcc agaaggtttc tcaaagagtc gagcatgttt
    24661 cgtcgtttcc tttatctagc agggtgtcgt gcggcgcgga gccccgcggg gacggggcgc
    24721 gacccttaca cccagccgcc gagaccggcg aggagaacga cgagcgggca gaaaaggagt
    24781 gctacgagga cgggaagatc gctcgtttcg ccacgaacac tgtcctttac gactctcgcc
    24841 cgcagaaccc aaatggggat gcagcaggcg gaggccaggg tgcccgagcg tcgggagatg
    24901 ctgaccgtga gctggccgat acagcctgca gcaccgaaca gcgcgagcat cggaaagcca
    24961 tattcgaagt tggcgtagac ctcttccctg gagaagaagt tgaccagcaa cccgagtaac
    25021 acgagcccga aggcggggga gaataccccc cgcaacgctc ctgtaacggc taccgcacgc
    25081 cttttctcga acttactgtt cagacaaggt ctccgacaga ggtttgggcg acgacattcc
    25141 tcgtagcccg agtcctgagt ctagcacttt tccgaccgcc gacgattgga gcctgcgcgc
    25201 ctgtaatagg tggggtctgg cctgcgcgaa cggcctctct gcgataagct acatgccccg
    25261 cccgcgggat gaagggattg agctcatgag aggaggaatc cgtggctgta gagacggaga
    25321 ccatcaggaa cgtgtgcctg atcgggcacc gcggaagcgg caagacatcc atagccgagg
    25381 gtatgatcgg gctggcttcg ggacgaagcg gtccgacgag cggcgtcctg gactacaccg
    25441 acgaggagac cgagcggggc atgacgctcg gtatgagcgt cgccaacctc cagtggaagg
    25501 ggcgccaggt caatgtgctc gacacccccg gagacggggg cttcgtgggg gacgctttca
    25561 tagcccagag ggtggccgac tgtgcgatcc tggtagtcca cgcccaggac ccgatccagg
    25621 tcgttaccga gcgcgtgtgg cgcagggggg agaaggaagg cataccccac gtcatcgtcg
    25681 tcaaccacct ggatagggag cgcacggact tcggagccgt agtagacgag ctcagggaac
    25741 gcttcggcca gcccgtcgtc ccgctcaatt tgcccatcgg acgggagaac gatctcaagg
    25801 gcatctacgg gctcctcagc ggaacctcct tcgtggacgg cgagcagcgg gcagagatcc
    25861 ccgagggcat ggaagacgag gtagacgagg ccaagacgca actcttcgag gccatagcag
    25921 agagtgacga cacgctgctg gaaaagtacc tagaagggga ggagatcacc accgaagagg
    25981 ctttcgacgg gatccgcaag gggatagccg agggcctgat catccccgtc ctcgcggcga
    26041 gcgcggagcg catgatcggc atagaccgcc tgctcgacgt gatagccggt agcgccccaa
    26101 gcccttccga cctggagcgt tggatcacgg aagagagcga agaaatccct tgcgatcctg
    26161 aaggaccctt cgcggcctac gtgttcaaga cttacgtgga cccgtacgcc ggccgcctga
    26221 gcgtccttag gatcgtgggc ggtcgctgcc gctcggacga ggcgctcacc aacccacgta
    26281 ccgattcttc agagagactt ggaggcatct cgcacctagt cggcaaggag cgcgagcctg
    26341 tagacgaggc cgtcgccggg gacatcatcg ccgtcgccaa gcttcgcgat acggcgacct
    26401 tcgacactct ttgcaagccc gagaagccga tgtctttcga accggtcgag cttcccgagc
    26461 cgacggcggc tttcgctgtc ggcgccaagg cacgcggtga ggaggagaag gtcttcgacg
    26521 cgataaggcg cgtggtcgat gaggatcctt cgctcaagct ggagcgcacc gaggccacgg
    26581 gcgaggacat cctgtccggg ctcgcccaga tgcacgtcga gctcgcgctg gagcgcatcg
    26641 agcaccgcta cggggtcgag gtcgaggcaa ggccgccgaa ggtgcccctt atggagacga
    26701 tcacgggctc ggcccaggcc cagggccgct acaagaagca gaccggtggc aggggccagt
    26761 tcggcgacgc gaggatcgag ctctcaccgc tgccccgcgg ttcgggcttc gagttcgaga
    26821 acggcatcgt cggcggcgcc atcccgcgcc agttcatccc cgcagtggag aaggggatca
    26881 tcgaggcgat ggccgggggg aacatcgccg gctaccccgt ggttgacgtg aaggtgcgcc
    26941 tgcacgacgg cgcgtttcat agcgtcgatt cctccgagaa tgcgttcaag gtcgccgggt
    27001 ctatggcctt caagaacgct gtcgagaaag cccatcctgt gctgctggag ccgtacgtga
    27061 aggtcgaggt gctaacacct tctgagctgg tcggcgacat catgggcgat ctctccagcc
    27121 gccgcggaag gccgatgggt gtcgagcagc gcggcgagcg tcagatcata caggccgagg
    27181 tgccccagat agaaatgctc acctacgccc gcgacctccg ctccataact ggcggacgcg
    27241 ccaacttcca tgtcgaggcc ggccactacg aagaagtccc cccgaacctc gtagaaaaag
    27301 tcctcgccgc caacgagagg gaggaagaga aagcagcgag ttaggtttca gcgagaacaa
    27361 gacctgacaa gctgaaatgt tatgaacgcg gtacgttgac cccgatcagg gagttgcggg
    27421 tgatgagttc cgcgagctcc tcctcaccca ggtcctctgt gtcgggcggg cgcataggga
    27481 ggaccatgta gcggacgtct gcggtgctgt cgtggacgat gatctcgact ccttcgggag
    27541 gttcgaagcc gaactcgcgc atcacggcgc gcggttccct gacggcgcgc gagcggtact
    27601 cgaagctctt gtaccagtct ggtgggcggc cgaggatggc ccgcgggtag caggagcata
    27661 gggtgcagac gataaggttg tggagttccg gcgtattctc gacgacaatg aactcggcgg
    27721 ggtgctcggg ggccatgccc agctccgaag ctgcggcctt accatcggag agcagtaacg
    27781 ccttgaaatc cgggttcacc caggcgcggg ctacgagctt ggcgccgttg gccggggagc
    27841 gggcctccat gtaggagatg ctgtcctgca cctccggctc agtgagaacg cctttctcga
    27901 ggagcagcgc ctcaatggcc ctgatgcgcg cggcgtaagg actcatgatc cctctcggct
    27961 cctcatgcga gtgatggtcg ctcacgtggt tcccctttcg ggctcaagcc agtgctcgta
    28021 gatgtcggcg tacaagtagt cttcggcagg acctctgtag tcgtcccaga ggtcggtttg
    28081 cctgaagccg accttgtaca ggggccgttc cggcagaccc gagaggccgt aggccacctc
    28141 ttccgggttg gggaacgcgc cgatcaaaga ctcgatccac ccggcctttc ccttgaggta
    28201 cgccggagtg cgtacgtggc caggcttctc cgagggaaga accctgacgc ggtcgccggg
    28261 ctcgaacgtc acggcccgtc ccagcgccgc tcgatctcgg ccgctctctc gtctatctcg
    28321 tcgctggaga ggacgtcctt ctcgatgagt agcttctcgg tcgccgcaac ccagcgttcg
    28381 tagtatgaga gctctcggta ttcttcgagg ctctctatag acctgcggag ctcgtctgtg
    28441 ctctggagcc ccttggcgcc gagcgccccg ttgatagcgt cggcgagacg ctcccagtcc
    28501 tcgacctcgt gctggctcct gtctatgggt cctccgggcg ccccgcccat gtcgtggacc
    28561 ccacccaagg tactgctcct atcctaccgt catgacttcc ttgatcgggg gtcgctcgac
    28621 gagctcctcg acgtactcct ggagcttcag tccgtccggc gtggcgaccg cgtgcaggta
    28681 gtctgagagc tggaaaagcg agtccggcat gcccgggtcc cgcacctggt tcaggcgtcc
    28741 ctcctggcgc atgcgcctgg cgacggcgcg aaggacagag taggccatgc gggagagatc
    28801 ccgcaggggt tggtgcctgt tctggcgggt accaaggtcg acctgcgcca ttgcctccag
    28861 cccgaccatc ttgaggacgt ctatcatgat cccggtctcg accgcatagc cggtgaggaa
    28921 tgggatggag gagaagagct ccctgtcggc gacgaactcc cctgcgaggg gttgcacgaa
    28981 gcctgtgagc tcggggtaga agaggttgaa gagaggtttg gccgagagct ccgtaaccct
    29041 ccctccgccg tcctgctcca cggtctcgta gctcttgaaa ggacgtctgt aggcggcctt
    29101 gacaaaacgg atctcgggta cctccaggat cggacccaga atgccgtaga tgagctgggg
    29161 cttgaagtct ttggtgtcag cgtcgatgta catgacgagg tcgccgcgcg cgacggagag
    29221 tgcgcgccac atggcgtcgc ccttgccgtg cgcgccgccg tggttgctca tgagctcgtt
    29281 ctccgagtag acctcagcgc ccttcgatgc ggccacctcc gcggtaccat cgggtgagtc
    29341 agcgtcgacg gcgaggatct ggtccaccag cggatgatcg tccgtcaggc agttgacggc
    29401 gtggatctca tcgacgatcc cgcctacggt gtcggccacg ttgcggcagg gcaggaccgc
    29461 gctgaccgtc aggccgaggt cgcgcttgcg ggagcccagt gcctccaggt ccgagaactg
    29521 cctgtagtca taggagcgat gcttaaacca ctctacggcg ttcacaacat cacactcctt
    29581 acctacgtct ctccatcaca gccacggtaa gctaccgtat atgtgcggct ttgtccattc
    29641 ttttttctta atgttacaca tagcccattt ctataccgga taccgccggt tcacttgcct
    29701 ggccgccggg gacatcaggg ccaggatgat agaatcagga cccttttgga cgataataac
    29761 ctccggactt acggagagtt tgactatgcg catagtattg accaacgacg acggcatcga
    29821 ggctccagga cttctggcgg ccaggcgggc gctggagaag gtgggggacg tcatcaccgt
    29881 ggcccctgac cagaaccgtt cgggagtagg taggagcatc tccttcgggc gctcgctcca
    29941 cgttgaggag cgcgagatgg cggacggagt gacgggatac gcctgcacgg ggacgccggt
    30001 cgattgcgtg cgcctcgtcg ccctcgggct catggacttc g
//
LOCUS       DASTKM010000028         4642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_708926, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000028 DASTKM010000000
VERSION     DASTKM010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708926"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..147
                     /locus_tag="VFQ10_01095"
     CDS             19..147
                     /locus_tag="VFQ10_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET9963963.1"
                     /translation="MFGQGERGIMEFRYDFPGLFMFHAHVSEFAELGWMGLFDVKE"
     gene            151..1326
                     /locus_tag="VFQ10_01100"
     CDS             151..1326
                     /locus_tag="VFQ10_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET9963964.1"
                     /translation="MGRERGLVWVLLGLAPLLGLGVLLAAIVLNGAGIDREDRPPVED
                     LTIDRVLLPTPDEIVVRVTNGGPEPVSVSQVTVNEALWDFRVEPDATIPPLGSAEITL
                     PYSWVEGEAYGITLITGTGTTFSAEVPAAVITPTFSAETFWRYALIGTYVGVIPVALG
                     LLWYPFLRRLGSAGIGAVLALTLGLLVWLVVDTLLEAVEVAGRLPGVFSGVPLVLVAA
                     LLAFLGLLGAERLFSRRGETSRLSTSYRIALGIGLHNLGEGLAIGAAFALGEVALGTF
                     LVVGFTLHNITEGVGIAAPILEERPHLAHFAGLALLGGGPAVLGTWIGGFAYSNLASA
                     VLLAVGAGAILQVIYEVTRLLLKDSARSKSPALSAPNLGGFTAGVAIMYATALLVSV"
     gene            complement(1487..2152)
                     /locus_tag="VFQ10_01105"
     CDS             complement(1487..2152)
                     /locus_tag="VFQ10_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET9963965.1"
                     /translation="MATKRTYDDGCAAAHALDLVGERWALLVVRELLLGPKRFTDLRA
                     GLPQASPNVLAQRLRGLEEAGIVRRRRLPPPAASKIYELTEWGEELEPVIIRLGRWGA
                     RSPSKPEDAPLGVDSLILSFRTMFDPGAAEGFEAIYELRMGEDRFRAEVSGDSFEVER
                     GSVERPDAIIEADPATLGELVYQDRPLAEALRSGDVKIEGDDAAVERFLTLFTLPEPA
                     ALV"
     gene            2330..3775
                     /locus_tag="VFQ10_01110"
     CDS             2330..3775
                     /locus_tag="VFQ10_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET9963966.1"
                     /translation="MRDKNRWIALYVLCVGMLMIVLDATVVNVALPAIQNDLGFSQSS
                     LAWVVNAYLIAFGGLLLLAGRLGDLISRRGMFLAGIGVFTVASLLCGLAQSQELLVVA
                     RFVQGAGGAMTSAVILGMIVTMFPETREQAKAIGVFAFVASAGGAVGLLVGGLLTQSI
                     SWHWIFFVNVPIGIATAVLAGRLIERDKGIGFGDGADVSGAFLVTGSLMLGVYTIVNP
                     AAEYGWGSGRTLTIGALSLVLLAAFVAREATARNPLVPLRIFRSRNVTGANLIQVLSV
                     AGMFGMFFLGALYLQRILAYDALQTGLAFLPVTILMGTLSVRYTDRLVMRFGARKLVL
                     GGLALFMAGLALFARAPVNGDYVVHILPVMVLLGIGAGLCFPALMTLAMSGATPSDAG
                     LASGLINTTAQVGGALGLAVLATLSASRSEQLIGDGESTAAALTSGYHLAFGIGAVLV
                     VVAIAVAVSVLQPERQTEEEFQVEPADCEAA"
     gene            3867..>4642
                     /locus_tag="VFQ10_01115"
     CDS             3867..>4642
                     /locus_tag="VFQ10_01115"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9963967.1"
                     /translation="MNDIQQLVQRVVDELVESGAERGLQVAVYRNGEQVADAVAGVAD
                     PETGRPVTPDTPFYCYSVGKGATSTVAHVLVERGLFGYDTPIVDLWPEFGAHGKEAAT
                     VRHALTHTVGVPGIPADTTPEDLCDWDKMCAAIADAEPWWEPGTKIAYHAYPFGYIVG
                     EIVRRTTGKPISRVLREEVAAPLGITDEIYFGVPESELGRLAKLEDMEGSAEMLASMP
                     DDSPFFKLGPRQVTPTAEFGNRHDILMADIPAGGKMSARAV"
BASE COUNT          771 a         1403 c         1541 g          927 t
ORIGIN      
        1 gagttcacgg acaccaaggt gttcggccag ggggagcgcg gcatcatgga gttccgctac
       61 gacttccccg ggttgttcat gttccacgcc cacgtcagcg agttcgccga actcggttgg
      121 atgggcctct tcgacgtgaa ggaataggag atgggacgag agcgtggact ggtctgggtg
      181 ctcctggggc tcgcaccact gctggggttg ggcgtgctgc tggccgccat cgtgctgaac
      241 ggtgcgggga tagaccgtga ggaccgtccg cccgtcgagg acctcaccat cgatcgggtg
      301 ttgctgccta cgccagacga gatagtggtg cgtgtgacca acggcggtcc cgagccggtg
      361 agtgtgagcc aggtgacggt gaacgaggca ctatgggact tcagggtgga gccggacgcc
      421 acgatcccgc cccttggttc ggcggagatc accttgcctt actcctgggt cgagggcgag
      481 gcatacggta taaccctcat tacgggcaca gggaccacct tttccgctga ggtgcctgcg
      541 gcggtgatca caccgacgtt ctcggcagag acgttctggc gctacgcgct gatcgggacc
      601 tacgtcggcg tgataccagt ggccctcggg ctgctctggt acccgtttct cagacgcctc
      661 ggctccgcgg ggataggggc agtacttgcc ctcacgctgg ggctcctggt ctggctggtc
      721 gtggacacgc tgctcgaggc agtggaggta gccggacggt tgcctggggt cttctccggc
      781 gtcccgcttg tgctggtggc ggcactgctg gccttcctcg ggctgttggg ggccgagcgg
      841 ttgttcagta ggcgcgggga gacgagccgg ctctcgacat cataccggat tgccctgggg
      901 ataggcctgc acaacctggg ggagggactt gccatcggcg cggcattcgc cctcggggag
      961 gtggcgctgg ggacgttcct cgtggtcggg ttcacgttgc acaacattac ggagggtgta
     1021 ggcatcgcgg cgcccattct cgaagagagg ccacacctcg cgcacttcgc tggcttggcg
     1081 cttcttggtg gggggcccgc ggtgttgggg acgtggatcg gcggcttcgc ctactccaac
     1141 ctcgcctcgg ccgtattgct cgctgtcggg gccggcgcca tactccaggt gatctacgag
     1201 gtcacgcggc ttctccttaa ggattccgca cggtcgaagt cacctgccct ctccgcccca
     1261 aacctgggcg ggttcaccgc cggggtggcc atcatgtacg ccacggcttt gctggtgagc
     1321 gtttaggccg actgtggggg ctcgacacgg gtcgaggtga aggccgccag caaggactat
     1381 agtaagaact ggctgtacct tcgagacgca cgtgccctca ggcggagccc tactacctgg
     1441 ctatccagtc gcactccgag gaactgcaga taaggacaca ctaccgttac acgagtgcgg
     1501 caggctcagg tagggtgaac agcgtgagga aacgttctac agccgcgtcg tcgccttcga
     1561 tcttcacgtc cccggatcgc agtgcttctg ccaggggccg atcttggtag acgagctcgc
     1621 ccagcgtagc cgggtcggcc tcgattatcg cgtcgggccg ctccacgctt ccgcgttcga
     1681 cctcgaagct gtccccggac acttcggccc tgaagcggtc ttcccccatc ctgagttcgt
     1741 agatcgcctc gaagccctcg gcagcgccag ggtcgaacat cgtcctgaag gagagaatca
     1801 gagagtcaac acctagggga gcgtcctcag gtttggacgg ggagcgggca ccccacctgc
     1861 cgagacgaat aataaccggc tccaactcct cgccccactc ggtcaactcg tagatcttcg
     1921 acgccgccgg cggaggaagc cttcgacggc gcacgatccc agcctcctcc aacccgcgca
     1981 ggcgctgggc gaggacgttg ggactggctt gcggcaggcc agcccgcaag tccgtgaacc
     2041 gcttcggccc caacaacaac tcgcgtacta ccaaaagcgc ccagcgctcg ccgaccaggt
     2101 cgagtgcatg cgccgccgca cacccgtcat cgtatgtgcg ttttgtcgcc ataccggaga
     2161 gtatagcacc gtaagtactg aagtctacat atgtagttag atattctaga gtttatggtt
     2221 gtttttagga ctattgccgt attctccaac caatggtact gcagtctcgg aaaatggagt
     2281 agagggaaat gacgaagcga gaggccagaa cgtgcgagga ggagaacctg tgagggacaa
     2341 gaatcgttgg atcgctctat acgtactttg tgtggggatg ctgatgatcg tgctggacgc
     2401 cacggttgtc aacgtggcgt tgcctgcgat tcagaacgac ctcggtttct cgcaatcgag
     2461 ccttgcctgg gtcgtcaacg cctacttgat cgccttcggt ggcctgctgc tgctcgccgg
     2521 gcggctcgga gacctcatct cccggagggg gatgtttttg gccggcattg gggtgttcac
     2581 ggttgcctca ctgctatgcg gcctcgcgca gagccaggag ttgttggtag tggcccgctt
     2641 cgttcagggg gctggcggcg ccatgacctc cgcggtgatc ctgggcatga tcgtcaccat
     2701 gttcccagaa acgcgagagc aggcgaaggc catcggcgtc ttcgccttcg tggcctccgc
     2761 cgggggcgcg gtgggcctgc tcgtcggcgg cctcctgacc cagtcgatca gttggcattg
     2821 gatcttcttc gtgaacgtcc cgatcggtat cgcgacagcg gttctcgccg gacggctgat
     2881 agaacgcgac aagggcatcg ggttcgggga cggcgcggac gtctccggcg ctttcctggt
     2941 aaccggctcg ctgatgctag gcgtctatac tatcgtcaat ccggccgctg agtacggctg
     3001 gggctccggc cgcaccctga caataggcgc cctctcgctc gtgttgttgg cggctttcgt
     3061 cgcgcgcgaa gcgaccgcgc gcaacccgct ggtgccgcta cggatcttcc gctcgcgaaa
     3121 cgtcactggg gcgaacctga tccaggttct tagcgtcgct gggatgttcg gtatgttctt
     3181 tctcggggcg ctctatctgc agcgaatcct cgcctacgat gccctgcaga ccggtctcgc
     3241 gttcctgccc gtaactatct tgatgggaac cctgtcggtc cgctacaccg accgcctggt
     3301 catgcgtttt ggggccagga agttggtgct cggcggcctc gcgctattca tggccgggct
     3361 cgcactgttc gcgcgggcgc cggtaaacgg agattatgtg gtccacatct tgcccgtgat
     3421 ggtccttctc ggcatcgggg ccggtctctg cttccctgcg ctcatgacgc ttgcgatgtc
     3481 tggtgccacg cccagcgacg cgggtcttgc ctccggcctg atcaacacca cagcgcaggt
     3541 cggcggtgcc cttggcctgg ccgtgctcgc gaccctgtca gcgtcgcgga gcgagcagct
     3601 gatcggggac ggcgagtcca ccgcggcggc gctgacgagc ggataccacc tagcgttcgg
     3661 gatcggagcc gtactcgtcg tcgtggcgat tgccgtcgcg gtgagtgtgc tgcagcccga
     3721 acgccagaca gaggaagagt tccaagtgga acccgccgat tgcgaagcgg cgtaggttcc
     3781 aggccggcga gaatcctacg aacggtcttc aacctgacga gcggcacggt cgtagactag
     3841 agtatgcttt ggagaggaat gggatcatga acgacattca gcaactggtc cagagagtgg
     3901 tcgatgaact ggtcgaatcg ggggcggagc ggggcttgca ggtcgctgtg taccgaaatg
     3961 gcgagcaggt ggctgacgca gtcgctggcg tcgccgaccc ggagaccggg cgtcccgtca
     4021 cgccggacac gcctttctat tgctactcgg tcggcaaggg tgcgacctcg acggtggcgc
     4081 acgtgcttgt cgagcgaggt ctcttcggct acgacacgcc gatcgtcgat ttgtggcccg
     4141 agttcggggc tcacggtaag gaggccgcta ccgtgcgcca cgccctgacg cataccgtcg
     4201 gtgtgcccgg aatacctgct gacaccacgc cagaggacct gtgtgactgg gacaagatgt
     4261 gcgctgccat cgccgacgcc gagccctggt gggagcctgg gacgaagata gcctatcatg
     4321 catacccgtt cggctatata gtcggggaga tcgtgcgccg gactacgggc aaacccatct
     4381 cgcgggtgct tcgcgaggag gtggccgcgc cgctcggcat caccgatgag atctacttcg
     4441 gagtcccaga gtccgagttg ggcagactgg ccaagctgga ggacatggaa ggcagcgcgg
     4501 aaatgcttgc ctcgatgccc gatgattctc ctttcttcaa gctggggccg cggcaggtga
     4561 cccctaccgc cgagttcggc aaccgtcacg acatcctgat ggccgatata ccggccggcg
     4621 gcaagatgtc agcgcgtgcc gt
//
LOCUS       DASTKM010000029         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1061435, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000029 DASTKM010000000
VERSION     DASTKM010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061435"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFQ10_01120"
     CDS             <1..801
                     /locus_tag="VFQ10_01120"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565854.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HET9963968.1"
                     /translation="LEARGVHVAYGIVGLKTHAKISLVVRREGSGLRRYVHLGTGNYN
                     PGTARIYTDFSYFTDAPKLAEDGSDLFNYLTGYSEQEEYQELLVAPLTMRESIERLIE
                     EQTEKAKGGEPASITCKTNSLTDPQIIELLYEASQAGVKIDLIVRSICCLRPGVEAVS
                     ENIRVVSLVGRFLEHARIFAFSDGDDEKMYLGSADLMQRNLDRRVETAFPLREKRHRE
                     KVRRLLDLQLADNANGWELGPDGNFERLHPKEGKEYVDSQAILLEEGF"
     gene            916..1203
                     /locus_tag="VFQ10_01125"
     CDS             916..1203
                     /locus_tag="VFQ10_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963969.1"
                     /translation="MTTGQSSGSSRTSEPSATDHQDAVVAAAYLRELGTKTGEARVPR
                     GVAFTMGVYAERCTREAKDLRSVVPDSKPFGTRTKGKKWKHFEKVLERRSG"
     gene            1235..1741
                     /locus_tag="VFQ10_01130"
     CDS             1235..1741
                     /locus_tag="VFQ10_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9963970.1"
                     /translation="MYVYLIRHAVAHKRDRERWPDDSRRPLTPGGEEEFVGAARGLAR
                     IVPEVDVLLSSPYERAWRTAEILAEQTGWPAPEKLPALEPDVPPEKVVIALQTYQGNQ
                     SIALTGHRPCLHELAVYLLTGDAGGADLKIKKGGVACIEFDDTPKAGAGKLRWLFTPK
                     VLRNLTIS"
     gene            complement(1783..2463)
                     /locus_tag="VFQ10_01135"
     CDS             complement(1783..2463)
                     /locus_tag="VFQ10_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HET9963971.1"
                     /translation="MEEYDRVWERFVGERRLEFGGYMDPDWQEGYRLSASLIVPGEAS
                     RFHERLEPLREALRPFPFVSLHPDHFMHITLVLLGFLVGEPKEEDEVSRERLQEIEER
                     ARIALSDVPAFTVRFANLNAFPGAAFIEAHAGGMIDRLRDALCTSCGLKKPPGPPHLT
                     LAYFEAPDGTVAPEELISTIARYRDWPIGEIVVENLEMTLLDLRLEYPEPETLARIPL
                     GKALQHTS"
BASE COUNT          545 a          811 c          795 g          456 t
ORIGIN      
        1 ctggaggcca ggggcgtcca cgtcgcctac ggtatcgtcg gcctcaagac gcacgccaag
       61 atcagcctgg tcgtcaggcg cgagggctcg ggcctgagac gctacgtcca ccttggtaca
      121 ggaaactaca atcctgggac ggcccgcatc tacacggatt tctcgtactt caccgacgcc
      181 ccgaagctcg ctgaggacgg ctcggatctc ttcaactacc tgacgggcta ctccgagcag
      241 gaggaatacc aggagctact cgtcgccccc ctgaccatgc gtgagagcat cgagcgcctt
      301 atcgaagagc agaccgagaa ggccaagggc ggcgagcccg ccagcatcac gtgcaagacc
      361 aactcgctaa cggaccccca gatcatcgag ctcctctacg aagcctccca ggccggggtg
      421 aagatagacc tgatcgtacg cagcatctgc tgcctcaggc ccggcgtgga agccgtgagc
      481 gagaacatcc gcgtagtctc gctggtagga cgcttcctcg agcacgcccg tatcttcgcc
      541 ttttccgacg gggatgacga aaagatgtac ctgggaagcg ccgacctgat gcagcgcaac
      601 ctcgaccgtc gcgtggagac ggcattcccg ctgcgcgaga agcgtcaccg cgagaaggtc
      661 cgccgcctgc tcgacctcca gctcgccgac aacgcgaacg gttgggaact cggcccagac
      721 ggcaacttcg aacgcttgca cccgaaagag ggtaaggaat acgtcgacag ccaggcgata
      781 cttctcgagg aaggcttcta atcatcggag atgccgcaat atacacgttt gttgctagtg
      841 atccacgtcc gtgctaaatt gcgcctctgt cttcggagga acgcggctgg gacaggctaa
      901 cgaaacgagg cgcgcatgac aacgggacag agcagcggat catctcgtac gagtgagcct
      961 tcggctacgg atcaccagga cgccgtggtc gccgccgcat acctgaggga gctcggcaca
     1021 aagaccggtg aagcgcgggt accgcggggg gtcgccttca cgatgggcgt ctacgcagaa
     1081 cgctgcaccc gcgaggcgaa agacctgcgt tctgtcgtgc ccgactcgaa acccttcggc
     1141 acccggacga aaggcaagaa gtggaagcac ttcgagaagg ttctggaaag acgaagtggc
     1201 tgacatacca agcaagggtg ttcggtaggc ccgcttgtac gtctacctca tccgccacgc
     1261 tgtcgcccac aagcgtgacc gggaacgctg gcctgatgac agtaggcgtc cgctcacgcc
     1321 tgggggcgag gaagagttcg tgggggcggc ccgcgggctg gcccgtatag tccccgaagt
     1381 tgacgttctc ctgagcagcc cctacgaacg ggcgtggcgg accgcggaga tccttgccga
     1441 gcagacaggc tggccagcac cggagaaact ccctgccctg gaacctgatg tcccgcccga
     1501 aaaagtcgtc atcgcgctac agacctacca ggggaaccag tctatagccc tgaccgggca
     1561 caggccctgc ctgcacgagc tcgccgtgta cctgctcacc ggcgatgctg ggggcgcaga
     1621 tttgaagata aagaagggcg gcgtcgcgtg catcgagttc gacgacaccc cgaaggccgg
     1681 agccggtaag ctgcgctggt tgttcacccc gaaagtcctc cggaatctaa caatcagcta
     1741 gtcagccagg gcttttgcgc ccgcgactag ctgcattgct gactagctgg tgtgctgaag
     1801 tgctttcccc agcggtatcc gggccagcgt ctcgggctcc gggtactcca agcggaggtc
     1861 gagcagggtc atctccaagt tctctaccac tatctcaccg atgggccagt ctctgtagcg
     1921 tgctatggtg gagatgagtt cctcgggcgc aaccgtaccg tccggcgcct caaagtaagc
     1981 gagggtaagg tggggcggac ctgggggttt cttcaagccg cagctcgtgc acagggcatc
     2041 ccgcagccgg tcgatcatcc ctccggcgtg cgcctcgatg aacgccgcac cggggaaggc
     2101 attgaggttc gcgaacctga cggtaaaggc tggtacgtca gacaaggcga tcctggcgcg
     2161 ctcctcgatc tcttgcagcc tctcgcggga gacctcgtcc tcttcctttg gctcgcctac
     2221 gaggaagccg agaagtacca acgttatgtg catgaagtgg tcggggtgta gcgacacgaa
     2281 cgggaaagga cgcagcgcct ctctcagtgg ttcgaggcgt tcgtggaacc gtgaagcctc
     2341 cccagggact atgagagatg ccgagagcct gtatccctcc tgccagtcgg ggtccatgta
     2401 cccgccgaac tccagacggc gttcaccgac gaagcgctcc cacacccgat cgtattcctc
     2461 catccacaaa ctctaacaga gagaagcttc tacccgctca aggattatcc ctgatgcggt
     2521 gttcacaata gaatctgttc agccagggcg actcgcagta cgttagtacc cttgcgtatc
     2581 gccctttcgg atacgagcga tcaggcg
//
LOCUS       DASTKM010000030         5324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1414063, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000030 DASTKM010000000
VERSION     DASTKM010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414063"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFQ10_01140"
     CDS             complement(<1..753)
                     /locus_tag="VFQ10_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HET9963972.1"
                     /translation="MRTLRRGDRGREVVDLQTRLQALGFDLGSRGIDGVFREKTELAV
                     RVFQQRLGLLADGLVGPITWREIVEAGYRPGGRLLYLRQPPFRGADVVELQQMLDDLG
                     FDPGAVNGLFDSRTVRAVRDFQRNAGLQDDGVVDATVFRVLNTYAAQSLGTHQLPDKN
                     GGYFTDDLFSGAIVVDAAHGGRDTGYESPTGLSEADLNLAIAQELAQILPAREVLLTR
                     SGDEEVSAVDRAFLANSSGARMIVSVHHASHPT"
     gene            complement(867..1157)
                     /gene="groES"
                     /locus_tag="VFQ10_01145"
     CDS             complement(867..1157)
                     /gene="groES"
                     /locus_tag="VFQ10_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET9963973.1"
                     /translation="MKFKPLGARALVQIVEREETTASGIVLPDTAKEKPQTAKVVAVG
                     EGKLDKDDQRVPVEVSQGDVIVFAKYSGTEISLDGEDYMILDADDILGIVES"
     gene            complement(1277..1588)
                     /gene="trxA"
                     /locus_tag="VFQ10_01150"
     CDS             complement(1277..1588)
                     /gene="trxA"
                     /locus_tag="VFQ10_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9963974.1"
                     /translation="MAVAEVTDATFESDVLRSERPVIVDFWAPWCVPCRRVSPILEEM
                     SESRDDVAFVKVNVDDNPQAAMNYSISSIPTIARFEEGQVTQQAIGALPKNQLSAQLG
                     L"
     gene            complement(1676..2635)
                     /locus_tag="VFQ10_01155"
     CDS             complement(1676..2635)
                     /locus_tag="VFQ10_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HET9963975.1"
                     /translation="MILTVTLNAAVARTLVVPSLTLGHRHRAPESMTLAGGKGVNVAR
                     SLRTLDVPVLAAGFAGGRNGDAIRDGLSEAAIPFDLVEIEGHSRTSTAIVDPMAGTQT
                     EINEYGSEVTPAEAREFSRRLEYLMEYATAVVFAGSVPPNLDESFFVGLVRRARERGI
                     YTVVDSPPSVLRAALKANPSLVSPNQHEAESVVGFDFIEEGDFPLGLTRLVELGAANA
                     CITSPEGLSYLSTENGTVSARSPRVEAASTVGSGDAYLAGLLAGLLHRNLAPAEAVRL
                     AAGCAAANAETLGAGLFEARRAEELADSVQVEVSSVSLQESRT"
     gene            3028..3588
                     /locus_tag="VFQ10_01160"
     CDS             3028..3588
                     /locus_tag="VFQ10_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9963976.1"
                     /translation="MKRLAIVFFIVLAAALFYRSFGSAETGTGSLTLPQPAPNVGDNA
                     PGFRVKSVDDRVFELSDHGVYVLTFWSTLNKDSNEAVSGFEDLAREYEDDEASFAVVY
                     VNSAPRDDNVPYTMLLDSTGELVSKYNVKRVPRLFVVEDGTIGYAQDYYYEGYENTLR
                     EAIDEALANEPGKGRQSALREQEPRL"
     gene            complement(3745..4647)
                     /gene="pyrF"
                     /locus_tag="VFQ10_01165"
     CDS             complement(3745..4647)
                     /gene="pyrF"
                     /locus_tag="VFQ10_01165"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET9963977.1"
                     /translation="MPWASPRGTLLFARSSGNQGTMQALVDAARRKQSVLVVGLDPIP
                     SRFPEELRALPEAEATLEFCRGILEAVEPYVAAVKLQAAFFERLGTVGMGIYAEVIEA
                     ARDLGLPAIADVKRGDIGPVAAAYAGAHLGVYGATCVTVNPYMGEDAVLPFLEEARRQ
                     NAGGGVFVLVATSNPSSREVQETTDPPLFEVVAGLVAGFGRSTGDYPDAGAVVGVTRP
                     EIGRRVRELLPYALFLSPGYGAQGGDASGVRPLLDSGGGGVLVNSSRSILYAYESSAS
                     GYREAAKEATREARDELRLTGARL"
     gene            complement(4667..4945)
                     /locus_tag="VFQ10_01170"
     CDS             complement(4667..4945)
                     /locus_tag="VFQ10_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9963978.1"
                     /translation="MALNKTALIEEIAGKANASKSDAQRFFDAFTEVVTDSLKEGEQV
                     QITGFGKFYVQKRDARQGINPQTKQRINIPASKVPKFTAGNALKDQIK"
     gene            complement(5022..>5324)
                     /locus_tag="VFQ10_01175"
     CDS             complement(5022..>5324)
                     /locus_tag="VFQ10_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET9963979.1"
                     /translation="ALATGLPYVIVRKSAKEHGSSAGRSIEGNLEKGERVALVEDVVT
                     TGTQAVRAARNLEETGSEVVMIVAVLDRREENGEELGGFPFRSLLRMDDLRVVKRN"
BASE COUNT          918 a         1745 c         1604 g         1057 t
ORIGIN      
        1 ggtcgggtgg gaagcgtggt ggacggagac tatcatcctc gcccccgagg agttggcgag
       61 gaaggccctg tcgacggccg agacctcctc gtcaccgctc ctggtcaaaa ggacctccct
      121 ggctggcagg atctgggcca gctcctgcgc tatggcgagg ttcaagtctg cttcggagag
      181 ccccgtcggg ctctcgtacc ccgtgtccct gccaccgtgg gcggcgtcca cgactatggc
      241 cccggagaac agatcatccg taaagtatcc tccgttcttg tcagggagct ggtgggtgcc
      301 gagggactgg gcggcgtatg tgttcaggac cctgaagacc gtggcgtcta caaccccgtc
      361 gtcctgtagg ccagcattcc tctggaagtc gcgaacggcc ctgacggtac gggagtcgaa
      421 caggccgttg acggcgccag ggtcgaatcc gaggtcgtcg agcatttgct ggagttcgac
      481 aacgtcggcg cctctgaaag gcggctgcct gaggtacagc aggcgtcctc ccggcctgta
      541 gccggcctcc actatctcgc gccacgtgat gggaccgaca agaccgtcag cgaggagacc
      601 gaggcgctgc tggaagaccc ttacggcaag ctccgtcttt tccctgaaga ccccgtctat
      661 gcccctgcta cccaggtcga agccgagggc ctgcaggcgg gtctggaggt cgacgacctc
      721 ccgccccctg tctcccctac gcagtgtacg cacgtacccg accggtctcc cctctttcct
      781 tgctcagacg tcgagccggc caaagcgtga gcgccgggca tcctcgcggg cccggcgctc
      841 cggagagccg actcttgctc tttcctctag ctctcgacta tccccaggat gtcgtctgca
      901 tcgaggatca tgtagtcctc gccgtcgagg ctgatctcgg tccccgagta cttggcgaag
      961 acgataacgt cgccctggga aacttcgacc ggcacgcgct ggtcgtcctt gtcgagcttg
     1021 ccttcgccaa ccgccacgac cttggcggtc tgtggcttct ccttggccgt gtcgggcagc
     1081 actattcccg aagccgtcgt ctcctcgcgc tcgacgatct ggacgagcgc ccgcgctccc
     1141 aaaggcttga acttcatttc tgtgtcctcc tcatcatttc ggactcatct acgacacccc
     1201 ttgcgacggc cgggcttacc cgactctacg gggcttacgc gccagagatt gtaaccggga
     1261 gtgttgctcc tcgcaattac aggccgagct gtgccgatag ctggttcttc ggcagggctc
     1321 ctatggcctg ctgggtgacc tgcccctctt cgaaacgggc gatggtcgga atgctcgaga
     1381 tgctgtagtt catcgctgcc tgagggttat cgtcaacgtt gaccttgacg aacgcaacgt
     1441 catcgcgcga ctcggacatc tcttcgagga tcggggatac ccgcctgcat ggcacgcacc
     1501 aaggcgccca gaaatccact ataaccggcc tctccgaccg cagaacgtcg ctctcgaaag
     1561 tcgcgtccgt tacttcggct accgccatga tctcgaacct ccgttgtctc tggaagtgaa
     1621 cttcgccgaa cattctagca cgccagcgcg ggcttctcgg aggcccgaac gtaccctagg
     1681 tccggctctc ttggaggctc actgaggaga cctcgacctg cacggagtcg gccaactctt
     1741 cggccctgcg cgcctcgaag aggcctgcgc cgagggtctc ggcgttggcg gctgcgcacc
     1801 ctgcggcgag acgaacggct tcggccggcg cgaggttacg gtgcagcaga ccagcgagga
     1861 gtccggcgag ataggcgtcg ccgctcccta ccgtcgaggc agcctcgacc ctcggcgagc
     1921 gcgccgagac ggtgccgttc tcggtgctga ggtaggagag gccctcgggg gaagtgatac
     1981 aagcattcgc ggccccgagc tcgacgagcc ttgtgaggcc gagagggaaa tccccttcct
     2041 cgataaagtc gaagcccacg acgctctcgg cctcgtgctg gttcgggctt acgagcgagg
     2101 ggttcgcctt gagtgctgcc ctgagcacgg acggcggtga gtctacaacc gtgtagattc
     2161 cacgctctct tgcacgtcgc acgaggccaa cgaagaagct ctcgtccagg ttcggcggga
     2221 cacttccggc gaagacgacg gccgtggcgt actccatcag gtactccagg cgcctggaga
     2281 actccctggc ctcggcgggg gttacctcgg aaccgtactc gttgatctcg gtctgcgtcc
     2341 ccgccatcgg gtcgactata gctgtcgagg tgcgggagtg tccctctatc tcgacgaggt
     2401 cgaacgggat ggccgcctca gagaggccgt cgcggatggc gtcgccattg cgcccgccgg
     2461 cgaacccggc ggcaagtacg ggcacgtcca gggtccttag ggacctggct acgttgactc
     2521 cctttccgcc ggccaacgtc atgctctcag gggccctgtg cctgtggccg agcgtcaggc
     2581 tgggcaccac cagcgtcctg gcaacggcag cattgagggt gacggtcaga atcatgcctc
     2641 tccctaccta tctcctgttc gaaaaatccc cccaacggta taccacaccc gctgcccgag
     2701 cgaggccttc tcataaccct tctttgcgag aagccgggtc tccccgacct tcttccttcg
     2761 aaccttatga aggcctcgcc gagtcagaca gatccgaggc gccctctccc ggcaggtact
     2821 ctccgctccg gaggtccgcc aaggccctgc ctggatatcg agcacgtccg tcacggcgag
     2881 cgtgacgaag aggcttatgg tcctggcttt cggcgtttcg aacacggggt tatagagact
     2941 acgatgatcc tggttctaca acaaggtcca tagtagcgga tacaatcctt atgccgtgtg
     3001 cctcttcaga cctggttcgg agggttcgtg aaacgcttgg ccatcgtctt cttcatcgtg
     3061 ttggccgccg ctttgttcta ccgttctttc ggcagcgccg agaccggcac gggctccctc
     3121 acgctacccc aaccagcccc gaacgtgggc gacaatgccc ccggattcag ggtgaagagc
     3181 gtagacgacc gcgtcttcga gctctcggac cacggtgtct acgtactcac cttctggagt
     3241 accctcaaca aggactcgaa cgaggccgtt tccggcttcg aagatcttgc cagggagtac
     3301 gaggacgatg aggcctcctt cgctgtggtc tacgtcaaca gcgctccgag ggacgacaac
     3361 gtcccctaca cgatgctcct ggactcgacg ggagagctgg tctcaaagta caacgtcaag
     3421 agggtgccca ggctgttcgt ggtcgaagac ggtacgatag ggtacgccca ggactactat
     3481 tatgaaggct acgagaacac tctcagagag gccatcgacg aggccctcgc gaatgagccg
     3541 gggaagggcc gccagagcgc cctccgcgaa caggagccca gattatagcc acgggcggaa
     3601 ggagaagatg agcagagaga gcatggtgca ggttcttggg gtcgtggtcg cggctgcgac
     3661 ggtgctagtg accgtgcttg cggtcgccca cctcttttct atttgatcgc ggttcgcctg
     3721 taggggcggg tttgaaaccc gcccctacag gcgggctccg gtcaggcgta attcatcccg
     3781 cgcttcgcgg gtggcttctt tggccgcttc cctgtaacct gaggccgagc tttcataggc
     3841 gtagaggata ctccggctgc tgttgacgag gactccgccg ccgccactgt cgagcagcgg
     3901 gcgcacgcct gaggcgtcac ctccttgggc cccgtatcct ggggaaagga acagggcata
     3961 gggcagcaac tccctcaccc tgcgcccgat ctcaggcctc gtgaccccta cgacagcccc
     4021 cgcgtctggg tagtcgccgg tcgacctgcc aaaccccgcc accagcccgg caacaacctc
     4081 gaagagcggc gggtccgtgg tctcctggac ctcacgggag gacgggttcg aggtcgccac
     4141 aagtacgaag acaccccctc ccgcgttctg cctgcgggcc tcctcgagaa acgggagcac
     4201 cgcatcctcc cccatgtaag ggttgacggt gacgcaggtt gcgccgtaga cgccgagatg
     4261 cgccccggcg taagcggcgg ctacgggacc aatgtccccg cgctttacgt ccgctatggc
     4321 cggcaatccc aggtccctgg cggcctcgat cacctcggca tagattccca tcccgacggt
     4381 acctagccgc tcgaagaaag ccgcctgcag cttgacggcg gcgacgtagg gctccaccgc
     4441 ctcgaggata ccccggcaga actccagggt cgcttctgcc tcgggcaggg cgcgcagttc
     4501 ttctggaaac cgtgatggta tggggtcgag gcccacgacg agcacgctct gcttgcggcg
     4561 cgccgcgtct accagcgcct gcatcgtgcc ctggttgcct gacgaacggg cgaaaagaag
     4621 ggtgccgcgg ggagaagccc acggcaccag aaaagaccct gggcagctac ttgatctgat
     4681 ccttgagcgc gttgcccgcg gtgaacttcg gcaccttgga tgctggtatg ttgatcctct
     4741 gcttggtctg cgggttgatt ccctgcctgg cgtcgcgctt ctgaacgtag aacttgccga
     4801 agcccgtgat ctggacctgc tcgccttcct tgaggctgtc cgtcacgacc tcggtgaacg
     4861 cgtcgaagaa cctttgggcg tcgctcttgg aggcgttcgc cttgccggcg atctcctcta
     4921 tcaatgccgt tttgtttaac gccacttctt cctccctgtt tcgcttccac tcttcttccc
     4981 tccggcctgc cggaaggatc taccagagac actatagcgc ctcaattccg tttaactacc
     5041 cgtaaatcat ccattctaag caaggaccgg aaaggaaatc cgccgagttc ttctccgttc
     5101 tcctccctgc ggtcgagtac tgctactatc attacgacct cggagcccgt ctcttcgagg
     5161 ttcctggcgg cccgcacggc ctgcgtaccc gtggtgacga cgtcctccac aagggctact
     5221 ctctcgccct tctccaggtt gccttcgata ctgcgtccgg cggagctgcc atgttctttg
     5281 gcagacttgc ggacgatgac gtagggcaac cctgtggcga gggc
//
LOCUS       DASTKM010000031         4005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1062163, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000031 DASTKM010000000
VERSION     DASTKM010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062163"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..604)
                     /locus_tag="VFQ10_01180"
     CDS             complement(92..604)
                     /locus_tag="VFQ10_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET9963980.1"
                     /translation="MESKAKIMGHPIHPILIPFPLGLLSTSVVFDVVYLLTGNGKWSE
                     ISFWMIAAGVIGGLAAAVFGLIDWLAIPSGTRAKAVGMWHGATNVVMVTIFIVSWLLR
                     ADAPGDPGIVAIVLSFVAVGLASLGGFLGGELVVRLGVGVAEGAHLNAPSSLSERAVG
                     RSSTGARREE"
     gene            complement(820..1377)
                     /locus_tag="VFQ10_01185"
     CDS             complement(820..1377)
                     /locus_tag="VFQ10_01185"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HET9963981.1"
                     /translation="MTSKTVAGRAPNIAIKRRSALGRLDILDVIAHTLHVILAGVWLG
                     GIIFTTTVVSPALKSVGWDEAEQARVRSVIGHQYAKIGTANLVLLLVFALMEGALSGF
                     GPALYSEYALVVVLFGLVAAHGAYFGRRLVELAEARKEARTFEKAQMLALRRRKLQRL
                     SFGVSMLNILISAAVMILAAYARVG"
     gene            complement(1565..2281)
                     /locus_tag="VFQ10_01190"
     CDS             complement(1565..2281)
                     /locus_tag="VFQ10_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HET9963982.1"
                     /translation="MKVFTPRRRNWATMLLGAYLFIAPWIFGTFGDEASSANAWIVGA
                     CIVVATLRVPIVSGPRAAELIRVGLGVWLLASPFALGFAGSAAAWNAWVVGALILTLA
                     DTLSLAFDFLSWLHAQKLCYQARRISPEKLLRYGEQEEPMSPEQLCWHLVECSREIRR
                     TLLGQTSGVVVGMCVLGYRACVNYGITLNRLIDTELPESGLARRLRLAVIRRQAAHSL
                     ERAREVIPPGVPHAWHRSRR"
     gene            complement(2278..3183)
                     /locus_tag="VFQ10_01195"
     CDS             complement(2278..3183)
                     /locus_tag="VFQ10_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HET9963983.1"
                     /translation="MATSDYAEVSYLEQEHETPKLLAGYLDRIGKEKLLTHREEKKLS
                     RRARAGDDRAREKLIQKNLRLVVSVAKRYRNYELPLEDLIQEGNIGLIRAVDKFDPEK
                     GYRFSTYATWWIRQAVGRAVSDKGRTIRLPVHIGEKIRKVGRVTSDLSVELGRKPTED
                     EVVERLGWTAEQVREVKGTMPDAASFNKPVSSEDEALRLEDFIVDETHLDVPDAVIEE
                     IEATWLKEAIGRLPQKARYVLVRRYGLDDRDTLTLAELAAELKLSRERVRQLQREGEF
                     LLKSGTRRAPHRSVARGSPLPKVLA"
     gene            complement(3170..3601)
                     /locus_tag="VFQ10_01200"
     CDS             complement(3170..3601)
                     /locus_tag="VFQ10_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963984.1"
                     /translation="MCEDKACSPEDGLEIRRQVQALDAASAAKRLREEKTYEDHGRSV
                     LNLFHGSSLRAMLTAIAGGRRTGERRVAGPSTVQVLEGEVRFRAGEEQHVLSAGGTLI
                     LEGNVPYEAEALSDAAFLHTLVRLTEGKSSRRKMKNGDGDI"
     gene            3769..>4005
                     /locus_tag="VFQ10_01205"
     CDS             3769..>4005
                     /locus_tag="VFQ10_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963985.1"
                     /translation="MRRSIASFAICGRRSVRRIHGWAEVLAALRKVTGGDRGFDRDRG
                     AQLSHFCVERLDTQPTSHGHPVITVADEVGVVDLQ"
BASE COUNT          765 a         1352 c         1094 g          794 t
ORIGIN      
        1 gcgtcttgcc ttctgccacc tcatgagcct acgccacacc tgccgatcca cctatcgaaa
       61 cgaacacttc gtgaaccact catagccttt cctattcttc tctccgcgcg ccagtcgaac
      121 tcctgccaac cgctcgttcg gagagcgagc ttggcgcgtt aaggtgcgct ccttcggcca
      181 cccctacgcc cagtcggaca accagctccc cgccgagaaa acctcccagc gaggccaagc
      241 ccaccgccac aaaggagagg acgatggcga cgattcccgg atcacccgga gcatccgcgc
      301 gaagcagcca gctcacgatg aaaatcgtca ccatgaccac gttggtagcg ccgtgccaca
      361 ttccgaccgc tttggcccgc gtaccggagg ggatcgccaa ccagtcgatc aggccgaaca
      421 ccgccgcagc cagaccaccg ataacgccgg ctgcaatcat ccagaacgag atctccgacc
      481 acttcccgtt tcctgtaagc aggtaaacaa cgtcgaagac gaccgacgtc gagagcaggc
      541 ccagtgggaa cgggatcagt atcgggtgta ttgggtgtcc cattatcttg gctttactct
      601 ccacaagctc tccttccatt cccacaacca ccgcccgtgg cgtctttttc gattgtgtac
      661 acgataccgt cttatcaaca ataaatcaag gaaatattta ttttattgta cataacggct
      721 gtcccggcgt tccggccgca cggtcgagaa ggtcgcccgg gagctccacc ccgcgaacaa
      781 cggtatccgg gctcatcttg catgggagcg tgggaaacct caccctaccc gggcgtaggc
      841 agcaaggatc atgaccgccg cactgatcag aatattcagc atggaaaccc cgaaggagag
      901 cctctgtaac ttccgccgcc tgagggccag catttgggcc ttttcgaaag tcctggcctc
      961 ttttcgagcc tcagccagtt ccaccaggcg tctcccgaag taggctccgt gtgcagccac
     1021 gagcccgaaa agcactacca ccagcgcgta ctcggagtaa agagcgggac cgaatccaga
     1081 gagagccccc tccataaggg caaaaaccaa aaggagtacg aggttcgcgg ttcctatctt
     1141 cgcatactga tgcccgatca ccgacctcac ccttgcctgc tcggcctcgt cccaccccac
     1201 cgacttgagc gctggcgaaa ccaccgtagt cgtgaagatg atccctccca gccacacccc
     1261 agccaagatg acgtgcagcg tgtgtgcgat tacgtctagt atgtctaacc tccctaacgc
     1321 cgatcttcgc ttaatcgcaa tgttcggtgc ccttccggca accgtcttcg aggtcatcgg
     1381 gcttcgacca tcgtgctact ctcaggactc cagaggatcc ctctgcctct atgctgctac
     1441 cacatctgta acagtagcgt gaggctgcag ctgtgtagta gtaccatgag ttcctcggta
     1501 gacgaaaagc accgtcttca gcgaccgggc tcatctctgg cgagcacccc gtgggggcag
     1561 caagctagcg cctactccgg tgccatgcgt gcggaacgcc tggagggatg acctcgcgtg
     1621 cgcgctcgag cgaatgggca gcttggcgcc ggatcactgc aagcctcaac cgccgggcga
     1681 gaccggattc cggcagttct gtgtcgatga ggcggttcag cgtgataccg tagttcacgc
     1741 aagcccggta gcccaagacg cacatcccga ctaccacccc ggatgtttgc ccgagcaacg
     1801 tccggcggat ctcgcgcgaa cactcgacga ggtgccagca aagctgctcg gggctcatcg
     1861 gttcctcctg ttctccgtac cggaggagct tctccgggga gatcctgcgc gcctggtagc
     1921 acaacttctg ggcatgtagc cagctcaaga agtcgaaggc gagactcagg gtgtcggcca
     1981 gcgtaaggat cagggcaccc acgacccacg cgttccacgc cgcggcggac cccgcgaagc
     2041 ccagcgcgaa cggcgatgcc aacagccaca cccccagccc gaccctgatc agctctgcgg
     2101 cccgcggtcc cgacacgatc ggcaccctta gcgttgccac gacgatgcaa gcgccaacga
     2161 tccaggcgtt cgccgagctt gcctcatctc caaaggtccc gaagatccag ggtgctatga
     2221 ataggtacgc cccgagcaac atggtcgccc aattacgccg cctgggcgta aataccttca
     2281 tgccaaaacc ttcgggagcg gggatccgcg cgcgacggag cggtgagggg ccctcctagt
     2341 cccagacttg agcaggaact cgccttcgcg ctggagctgc ctgacccgct ctcgcgacaa
     2401 cttgagctcc gcagcgagct ctgcgagcgt cagggtatcc cggtcgtcga gtccgtagcg
     2461 cctcaccagg acgtagcgtg ctttctgcgg caagcgtccg atggcctctt ttaaccacgt
     2521 cgcctcgatc tcttctatga cggcgtccgg gacgtcgagg tgcgtttcgt cgacgatgaa
     2581 gtcctcgagg cgcagagctt cgtcctcgct cgagacaggc ttattgaagc tggcggcgtc
     2641 cggcatggtt cctttcactt cccgcacttg ctcagcggtc cagcccagcc gctcgacgac
     2701 ctcatcctca gtgggcttcc gcccgagctc taccgaaagg tcgctggtga cgcggccgac
     2761 cttccttatc ttctcaccga tgtgcaccgg aagccttatg gtgcggccct tgtctgatac
     2821 ggctctcccc acggcctgtc tgatccacca ggtggcgtaa gtggagaagc ggtaaccctt
     2881 ctctggatcg aatttatcca cggctcggat aagacctatg ttgccctcct ggattagatc
     2941 ctcgagaggc aactcgtagt tgcgatatct cttggccacg ctgaccacca gacgaaggtt
     3001 cttctggatg agcttctccc gggcgcgatc gtctcctgct ctggcccgcc tcgaaagctt
     3061 tttctcctcg cggtgtgtca gcagtttttc tttgcctatg cggtccaggt agccggctaa
     3121 tagtttcggc gtttcgtgct cctgctccaa gtaagaaacc tccgcgtaat cagatgtcgc
     3181 catcgccgtt tttcatcttc cttcgagagc tcttgccctc ggtcagccgc accagagtgt
     3241 gcaggaaggc ggcgtcgctc aaggcctccg cctcgtaggg cacgttccct tcgagaatca
     3301 gcgtcccccc ggcggagagt acgtgttgct cctcccctgc ccggaacctc acctcgccct
     3361 ctaagacctg cacggtggaa ggaccggcta cccgcctctc acccgtacgg cgcccgccgg
     3421 cgatggccgt gagcatcgct cgtagagaag atccgtggaa tagattaagc acgctacggc
     3481 catggtcctc gtaggtcttc tcttccctga gtcgtttggc cgcgctcgcc gcatcgagcg
     3541 cctgcacctg cctgcggatc tcgaggccat cctcggggct gcaggcctta tcctcgcaca
     3601 tgtcggtaat gcctcctttc gcgtcgccaa tgtttgcatg ggccgcacac cgtccccacg
     3661 tggattgaca ccctccttgc gcggtcgacg gtgaatccca ttccctatcg ataacaggta
     3721 cgtcgaggtc ccttatgggc atgccctgtc accttccccg cgacgcgcgt gcgccggtcg
     3781 atcgcttcgt tcgccatctg cggcaggcgg tccgtacgac gtatccacgg gtgggcagag
     3841 gtgctggcgg ccctcaggaa ggtcacgggc ggagatcgcg gtttcgatcg ggaccgcggc
     3901 gcgcagctca gccacttctg cgtcgagcgc ctcgacaccc agccaacgag ccacggacac
     3961 ccggtgatta ccgtcgcgga cgaagtaggc gtcgtcgacc ttcaa
//
LOCUS       DASTKM010000032        14077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_181405, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000032 DASTKM010000000
VERSION     DASTKM010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14077
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181405"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFQ10_01210"
     CDS             <1..225
                     /locus_tag="VFQ10_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9963986.1"
                     /translation="TDILSTRMSGGYDRMSGTSMSAPFVSGLAGLLASRGMSADSIRQ
                     RMQTTAKDLGPAGDDPQFGHGRIDADNAVR"
     gene            334..1179
                     /locus_tag="VFQ10_01215"
     CDS             334..1179
                     /locus_tag="VFQ10_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9963987.1"
                     /translation="MATYTYNGKKSFAGEFFDSLAAFYRKRWLLRYFVRRQVTRSYKR
                     SYLGLAWAILGPLIWVFFLTLIFSEVVGLRFRQVEDDPTLNFGLFLYCGLLPFMAFSE
                     ALTKGLNSITSNAGLVQKVVFPLELLPFTNAVASLVDKLFGVGALVFVVFVLQHRLHW
                     QVLLLPLIIVLQLLFTLGLTYIMAVVGTYVPDMGEVLRPIIRGTFFLTPILWPPERLP
                     ENLRFIEDYNPLAYLVGAYRAMILEGTLPGGLATLYFALFSVALFLAGFALFVRAKPG
                     FADHL"
     gene            1176..2495
                     /locus_tag="VFQ10_01220"
     CDS             1176..2495
                     /locus_tag="VFQ10_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9963988.1"
                     /translation="MTPAISLRGIGKKYRISPNRSSRLGEILSFGKAKRSHEFWALQD
                     INLDVEPGTTLGVIGRNGAGKSTLLSIISGVLRPTTGEVEVRGRLSAIFGIGTGFNPL
                     FTGRENAMLSGLILGIEHDEMVERFDDIEAFADIGEFMDQPIKTYSSGMRSRLGFAVA
                     INVEPDVLVIDEALSAGDSAFKKKAIQRMYDLKDSGSTVLFVSHSMGMVKRFCTEAIL
                     LHKGRLVTTGSPEEVVDHYEQMLAKSQEMKNAGPSELDKQLDYEIEHEEEDDTPTKAE
                     DLGTPDHRRIVGGAEILGLELLDESGQPADALPSGASLTVRVRLRYDEAMEDSTLGIA
                     LQSERAGLEVFSTDTALEGAPLGRKEAGEQATVDFRLVMPLGPGIYKVDASLTDPQAE
                     DSYVARTQDAATLEITKEGALAPVRGLVRLPAKVEIHGQEGRRERPT"
     gene            2500..3504
                     /locus_tag="VFQ10_01225"
     CDS             2500..3504
                     /locus_tag="VFQ10_01225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9963989.1"
                     /translation="MPPRVTVVIPNWNGERFLRLCLGSLREQSFSAFETIVVDNGSVD
                     GSIPLVKEHFPEVNVIPLGENLGIASAFNAGIEASTLEYVALLNNDTEQDPGWLEALV
                     RAAEDHPEAGLFASKLVDFHDRGVLDGAGDAVRLSGLPYRLGHGERDSGRFDRPGYVF
                     GACAAAALYRRDMLDEIGLFDEDFVSYCEDGDLSFRAQLAGYRCFYVPGAVVYHMGSA
                     STGGKRSPTATRLGSRNSLSLLVKNLPLSAVPQILPFFAAGQLVRLITAAATGSLRAH
                     LEGLAGAWRHLPLMLGKRAEIQKMKKLSDAEVRRLLRESSLAATRSIARRLRDRAVWR
                     "
     gene            3501..4340
                     /locus_tag="VFQ10_01230"
     CDS             3501..4340
                     /locus_tag="VFQ10_01230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9963990.1"
                     /translation="MKFSVIIVNYASWPLTLRCIESLQQTRNGEFEVFVVDNDRVEPP
                     ELPSWVRLIRNKENIGFARAHNRGIAAASGDPIVLINPDTLVERDFFEHLEGFVSENP
                     MVGISGPRIVDSEGHLQLSARREISALSGFLGRTSLLTRLFPKSSFVKSQFPAVTDQS
                     HPTSVDWVSGACMVIRRETLRDVGPLDERFFMYFEDADLCRRARSAGWLVYYLPHVEI
                     VHQTGASSRSKPKAIWHLHKSAFLYHRKHGAHGPFGAYSAMVLAGLTLRATAKLGISL
                     FER"
     gene            complement(4344..4943)
                     /gene="rfbC"
                     /locus_tag="VFQ10_01235"
     CDS             complement(4344..4943)
                     /gene="rfbC"
                     /locus_tag="VFQ10_01235"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET9963991.1"
                     /translation="MNVIRTELSGVLVIEPDVFGDARGFFMESWNGARYEEHGIPEGF
                     VQDNLSFSAHGVLRGLHFQNPRAQGKLVWVLRGEAFDVAVDIRVGSPTFGRWTGSTLS
                     EENRRQLWVPPGFAHGFVVTGESALLSYKCTDYYAPQYDGSILWNDPEIGIQWPVENP
                     SLSDKDRAALPLNEMPEGTLPHYKEARDARSPSEIQPDT"
     gene            complement(4940..5821)
                     /gene="rfbA"
                     /locus_tag="VFQ10_01240"
     CDS             complement(4940..5821)
                     /gene="rfbA"
                     /locus_tag="VFQ10_01240"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET9963992.1"
                     /translation="MKGIVLAGGSGTRLYPLTRAVSKQLLPVYDKPMIYYPLSTLMLA
                     GIRDILVISTPEDLSRFEDLLGDGSQWGVDFSYAVQPRPEGLAQAFVIGREFVGSESV
                     SLVLGDNIFFGHGFSELLERSVGRQRGATVFGYYVRDPGRYGVVEFDGDGTVLSIEEK
                     PEKPRSHYAVTGLYFYDNRVLDVAADLRPSTRGELEITDVNVDYLERGELRVELMGRG
                     MAWLDAGTHESLHQSSNFIETIEQRQGLKIGCPEEISYRKGYIDSERLERLAHQMRKN
                     DYGRYLLEILKSEEYVI"
     gene            complement(5818..6879)
                     /gene="rfbB"
                     /locus_tag="VFQ10_01245"
     CDS             complement(5818..6879)
                     /gene="rfbB"
                     /locus_tag="VFQ10_01245"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET9963993.1"
                     /translation="MQREKQTILVTGGAGFIGGNFVLGAVGDYRVVNLDALTYAGNPD
                     TLASVSDNPDHVFVKGRIGDRGLVDRLLAEYRPSAVVNFAAETHVDRSIDGPKAFVQT
                     NVVETHELLEAALSYWSALQGEDHGAFRFLHISTDEVYGTLGTEGYFTEESPFRPSSP
                     YAASKAASDHFVRVYHHTYGLPTLTTNCSNNYGPYHFPEKLIPLMILTALRGEPLPVY
                     GDGQQVRDWLYVEDHCRAILAVLQRGRPGETYNVGGHNERTNLEVVKTICALLDELVP
                     GSPHESLITFVADRPGHDRRYAIDAGKIERELGWRPEETLETGMEKTVRWYLENRWWC
                     EAALSRGYRGERLGLGGPA"
     gene            7097..8164
                     /gene="thrC"
                     /locus_tag="VFQ10_01250"
     CDS             7097..8164
                     /gene="thrC"
                     /locus_tag="VFQ10_01250"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9963994.1"
                     /translation="MSVQARVSRQGVIRRYRGWLPEIPDDAIATIGEGDTPLIEAKRI
                     SERVGARLFLKFEGMNPTASFKDRGMTVAMSRAAATGSRACVCASTGNTAASAAAYAA
                     RAGMECFVVVPAGKIALGKAVQVLAHGARIVQIEGNFDVALKLSRRIAEDLGDVTMVN
                     SVNPDRIEGQKTAAFEVAEALGDPPDALALPVGNAGNITSYWKGFCEWRGTGHAGSAP
                     RMLGFQAEGASPLVAGHDFENPETVASAIRIGSPASKEGALSAARESGGLIESVTDGE
                     ILAAQEMLAREEGVFCEPASAAGIAGLLKLASEGRGPEGTVVSVLTGHGLKDPDVILG
                     RVQLPDPVPATFEAVAGRMER"
     gene            8170..9102
                     /gene="thrB"
                     /locus_tag="VFQ10_01255"
     CDS             8170..9102
                     /gene="thrB"
                     /locus_tag="VFQ10_01255"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET9963995.1"
                     /translation="MISVRVPATSANLGPGYDAVGLALSLSMRIGLDRSPHPEIDIKG
                     TGADLIPRGPEHPAYRAARFVAELVGESDAQFRLVQENSIPPTRGLGGSAAALVGGAV
                     AANDLFGGQIAAPDLLNIVCELDGHPDNAAPALLGGLVIGTLTPVGVSAVRLEPKDLK
                     VVVAVPDFAVSTTAARRALPDSVPHRDAAFNVGRSGLLLGALATGEYHLLRVAMQDRL
                     HQPYRSHLIPGLEDVIEAALKTGAHGACLSGSGPTVLAFAPETRARDIASSMKTTFEA
                     RGVGVESWVLDVDLAGARAEPPEEVTTLSPVPVL"
     gene            complement(9192..10415)
                     /locus_tag="VFQ10_01260"
     CDS             complement(9192..10415)
                     /locus_tag="VFQ10_01260"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9963996.1"
                     /translation="MAKNASTQGPSGGATIIDRRRFLKQTALATAVVSVPYLAGKTVA
                     FGKEGAKGADAALDRALEELVAMRGGPPGVIAIVQRGPHKEVHSFGVRNLNGDLPLRA
                     KDHMRIASAAKAFSGAVALSLVSKGTLSLHDTIGERLRELPKAWSKVTLRQLLNHTSG
                     VPDFSLDPDFQEALLASLKKAPPPEELLSFVEGEDLLFDPGSEYKYSNSDNIVVALMV
                     EAATGMSYEDQLRERVYSPLGLRKTTLPRGPNLRKPFIHGYDNDPSQERPEDLSEVVA
                     AGWAWASGGIVSTPADLNDFIRGYVGGELFDERTRAKQRRVVEGGSSEPPGPGKNSAG
                     LGIFRYETHCGTVWGHTGNTPGYTQFMAASPNGSRSVTVSVNTQLTPTDGEPGVFEAL
                     RRAEGLAVCAALAGT"
     gene            10891..12597
                     /gene="ilvB"
                     /locus_tag="VFQ10_01265"
     CDS             10891..12597
                     /gene="ilvB"
                     /locus_tag="VFQ10_01265"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HET9963997.1"
                     /translation="MENTAFNIEYKPRTGSEAMCEALLDEGVEYLFGYPGGAIMPFYD
                     ALPDYPLKHVLTRHEQAAAHAADGYARATGRVGVCVATSGPGATNLVTGLATAQMDSV
                     PLVAITGQVGRPAMGKDSFQETNVMGMTLPFTKHSFLVEDPDELYPTMRRAFEIARSG
                     RPGPVLVDVPKDVQFGATNDNGYRRLREAPPPVESPGLERAAMLLRSAKRPLILAGHG
                     VTLGGAEMELRLLAERTGIPVVTTLLGISAFPDDHPLYLGWPGMHGQASVNRAIDRSD
                     LLFAVGMRFDDRITGAAHKFAPGAKIIHIDIDPSELGKVIKPTVGIAADAKAAMRSIL
                     GHLDNAPHEGGGCKQWREELAKSQEPERRRKESARDEYGPIQVMEAIKGAAGSDLRLV
                     TDVGQHQMWAAQFFEFTKSNSHITSGGLGTMGFALPAAMGVAFAYPEDPVWVIAGDGG
                     IQMNIQELATIRDFGLNIKVAVLNNGYLGMVRQWQQFFHNRRYSETPITGPDYEQLTA
                     AYGVAGRTVREGDDVEAAVRWAQEQDGCTILDFHIDPEANVYPMIPSGMSVNEMIEED
                     GA"
     gene            12590..13042
                     /locus_tag="VFQ10_01270"
     CDS             12590..13042
                     /locus_tag="VFQ10_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9963998.1"
                     /translation="MPESATGVALNTIEVRFTGGMLALNRLLMTLQNKRMPVAGFTLG
                     SDSDGMRATVLLDCPPESALRYTALISALEDVREAGPAETIEVALIETSRDWRELAEQ
                     WGVEAHEDDGTVVASGEPDKVEAFLAAVGVEDAVRVGPLARPESGGGI"
     gene            13043..14065
                     /gene="ilvC"
                     /locus_tag="VFQ10_01275"
     CDS             13043..14065
                     /gene="ilvC"
                     /locus_tag="VFQ10_01275"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET9963999.1"
                     /translation="MARVYREGDIGNEITTRRVAILGYGSQGHAHALNLKESGCDVRV
                     GLYEGSKSWSKAEEDGLQVKTIAEAVREADVAMMLLPDEKQPAVFEAEVGPNLSEGDL
                     MLFAHGFNIHFNQIVVPPEVDLGLVAPKGPGHVLRRLYVEGKGMPSLFAVQNDASGQA
                     RDVILSYARAIGSGRAGIIETTFAEETETDLFGEQAVLCGGLTALLTAGFETLVEAGY
                     QPELAYYECVNELKLIVDLIYEGGLAGMRYSISNTAEYGDYTAGPKIIDEAARERMRS
                     ILADIQSGKWAKDWVLENQAGGSSFLAMRRREAESQVEKVGQELRALAAEGIETPAGS
                     AGGENL"
BASE COUNT         2560 a         4408 c         4555 g         2554 t
ORIGIN      
        1 acggacatac tctccacccg catgtcggga ggatacgaca ggatgagcgg aaccagcatg
       61 tcggcgccct tcgtgtctgg cctcgccggg ctgctcgcct ccaggggcat gtctgcggac
      121 tcgatccgtc agcgcatgca aaccaccgcc aaggacctcg gtcctgcggg ggacgatccc
      181 caattcgggc acggcaggat cgacgccgac aacgccgtga gatagtcaga gccgcagcct
      241 ggcaagcaca cccttgctta tgtaaacttg ccgctgttct cgggcacatg gcttacacta
      301 cgggcggcgc cgcctacgac acgagggacg taattggcca cctacacgta taacggtaag
      361 aaaagcttcg cgggggagtt cttcgactcc ctggcggcgt tctaccgcaa gcgttggttg
      421 ctgagatact ttgtccgccg ccaggtaacc cgtagctaca agagatccta tctgggcctg
      481 gcgtgggcca tactgggacc cctgatctgg gtcttcttcc tgacgttgat cttctctgag
      541 gtggtcggcc tgaggttcag gcaggtagag gacgacccaa cgctcaactt cgggctgttc
      601 ttgtactgcg gtctgctccc tttcatggcc ttctccgaag cgctgaccaa gggactcaac
      661 agcatcacga gcaacgcggg gttggtgcag aaggtggtct tcccgcttga gttgctccct
      721 tttaccaacg cggttgcttc gctggtggac aagctcttcg gcgtgggtgc gctcgttttc
      781 gtcgtgttcg ttctgcaaca caggctgcac tggcaggtct tgttgctgcc tctgatcatc
      841 gtgctgcagt tgttgttcac cctgggcctg acctacatca tggcggtagt cggaacctat
      901 gtgcctgata tgggcgaggt cctccgaccc atcataaggg ggactttctt ccttacaccg
      961 atcctctggc cacctgagag gttgccggag aacctgcgat tcattgaaga ctacaacccg
     1021 ctagcctacc tggttggcgc atacagggct atgatcctcg agggtacgct tcctggcggg
     1081 ctggcgacgc tctacttcgc cctgttctcc gtggccctgt tcctcgctgg cttcgcactg
     1141 ttcgtcaggg cgaagccagg gttcgcggac cacctgtgac gccggccatc tccctcaggg
     1201 gaataggcaa gaagtacagg atctccccga accggtcctc gaggctgggg gagatcctga
     1261 gcttcggcaa ggccaaacga agccacgagt tctgggcgct gcaggacatc aacctggatg
     1321 tggagccagg caccaccctg ggcgtcattg gccgcaacgg ggccgggaag agtacgcttc
     1381 tgagcataat ctcgggtgtt ctgaggccga cgacggggga ggtcgaggtc cgaggccggc
     1441 tgtcggcgat cttcggtatc gggacggggt tcaacccgct ctttacgggg cgcgagaacg
     1501 ccatgttgag cggcctgatc cttggcatag agcacgacga gatggtggag cgcttcgatg
     1561 acatcgaggc tttcgcggac atcggggagt tcatggacca gccgatcaag acctactcga
     1621 gcgggatgcg cagtcgcctg ggcttcgccg tcgcgatcaa cgtggagcct gatgtccttg
     1681 tcatagacga ggccctctcc gcgggcgact cggcattcaa gaagaaggcg atccagagga
     1741 tgtacgacct caaggactcc gggtccacgg tccttttcgt ctcccatagc atggggatgg
     1801 taaagcgatt ctgcactgag gccatcttgc tccacaaggg aaggctggta accacgggca
     1861 gccccgagga ggtggtcgac cactacgagc agatgctggc gaagtctcag gagatgaaga
     1921 acgccggccc cagcgagctg gacaagcaac tggactacga gatagagcac gaagaagagg
     1981 atgacacgcc gaccaaggcc gaggacctcg ggaccccgga ccaccgcagg atcgtgggag
     2041 gggccgagat cctcggcctc gaactgctcg acgagagcgg gcaaccggcc gacgcgctgc
     2101 cctcgggcgc gagcctgacc gtgcgggtac gcctacgcta cgacgaggcc atggaggaca
     2161 gtaccctggg cattgcgctg cagagcgaga gagccggtct tgaggtcttc tcgacggata
     2221 cggcacttga aggcgctccg ctcggacgga aggaggcggg cgagcaggcg acggtggact
     2281 tcaggctcgt gatgccgctc ggacccggca tctacaaggt ggacgcttcc ctgacggacc
     2341 cgcaagctga agactcgtat gtggcccgca cacaggacgc ggcgaccttg gagataacca
     2401 aggaaggggc tctcgccccg gtaagaggcc tggtgcggct gccagcgaaa gtcgagatcc
     2461 acggtcagga gggccggcgg gagaggccaa cctgaagccg tgcctccccg cgtcactgtc
     2521 gtcatcccga actggaacgg cgagcggttc ctgaggcttt gcctcggctc actgcgagaa
     2581 cagtctttca gcgccttcga gacgattgtg gtggataacg gctccgttga cggctcgata
     2641 ccccttgtga aggaacactt ccctgaggta aacgtgatcc cgctcggcga aaatctgggc
     2701 atcgcatcgg cgttcaacgc cgggatcgag gcgtccacgt tagagtacgt ggcactcctc
     2761 aacaacgaca ccgagcagga cccaggctgg ctggaagcgc tggtgcgtgc cgctgaggat
     2821 caccccgagg ccggtctttt cgcgagcaag ctggtcgact tccacgaccg tggggtgcta
     2881 gacggagccg gggacgccgt gcgtctgagc ggtctgcctt accgcctcgg gcacggcgag
     2941 cgcgacagtg gcaggttcga taggcctggc tatgtcttcg gggcgtgtgc ggcggcggcg
     3001 ctctatcgca gggatatgct cgatgagatc gggctcttcg acgaggactt cgtctcctac
     3061 tgcgaggatg gggacctgag cttccgcgcc cagctcgccg gttatcgttg cttctatgtc
     3121 ccaggcgccg tcgtctacca catgggaagc gcctcgaccg gcggcaagcg tagcccgacg
     3181 gcgaccagac tcggtagccg caactctctt agcctgctgg tcaagaacct gcccctctct
     3241 gcggttccgc aaatactgcc tttcttcgcc gcgggacagc tcgtgcgcct gatcaccgcc
     3301 gcggcgaccg gctctttgcg ggcgcacctg gagggtttgg ctggcgcgtg gcgccacctg
     3361 ccgctcatgc tcgggaagcg ggccgagata cagaagatga agaagctctc tgacgccgag
     3421 gtccgcaggc tcctcagaga gtcatcgctc gctgcgacgc ggagcatcgc gcgccggctg
     3481 cgagacaggg ccgtgtggcg atgaagttct ccgtcattat cgtcaattac gcctcctggc
     3541 ctctgacgtt gcgctgcata gagtccctgc aacagacgcg taacggggag ttcgaggtct
     3601 tcgtcgtcga caacgaccgt gtggagccgc ctgagcttcc ctcctgggtg cgcctgatcc
     3661 ggaacaagga gaacataggc ttcgcgcgcg cccacaacag gggcatcgcc gcagcgagcg
     3721 gagatcctat cgtgctcata aaccccgaca ccctcgtgga gagggacttc ttcgagcatc
     3781 tggagggatt cgtctcagag aacccgatgg tcgggatctc cgggccgagg atagtggatt
     3841 cggaaggtca tctccagctc tcggcgcggc gggagatcag cgccctctca ggcttcctcg
     3901 gacgcacctc cctcctgacc cgcctctttc ccaagagctc tttcgtaaag agtcagttcc
     3961 ccgccgtcac ggaccagtcc cacccgacct cggtcgactg ggtctctggc gcctgcatgg
     4021 tcatccgcag ggagacattg cgagacgtag gccctctgga cgaacgcttt ttcatgtact
     4081 tcgaggacgc cgacctctgc aggcgcgccc gctcggcagg ctggctcgtc tattacctgc
     4141 cgcacgtcga aatagtccac cagaccggcg ccagcagccg cagcaaaccc aaggcgatct
     4201 ggcaccttca caagagcgcg ttcctctacc accgcaagca cggcgcccac ggccccttcg
     4261 gcgcttacag cgcgatggtt ctcgccggac tcaccctccg ggccacggcg aaactcggaa
     4321 tctctctgtt cgagagatag gcttcaggta tcaggttgta tttcggacgg acttcgcgcg
     4381 tctcgggctt ccttatagtg cggcagggtc ccttccggca tctcgttcag gggtagtgcg
     4441 gcgcggtcct tgtccgagag gctcgggttc tcgacgggcc actgtatccc gatttcggga
     4501 tcgttccaca ggatggaccc atcgtactgg ggggcgtagt agtctgtgca tttgtaggaa
     4561 aggagcgcgc tctcgcccgt gacgacgaaa ccgtgggcga agccgggggg aacccagagc
     4621 tggcgcctgt tctcttctga gagggtcgag cccgtccacc ggccgaaggt tggggagccg
     4681 accctgatat ccacggcaac gtcaaaggcc tcgccccgca agacccagac gagcttgccc
     4741 tgggccctgg ggttctggaa gtggagaccg cgaagcacac cgtgggccga gaacgagagg
     4801 ttgtcctgca caaagccctc cggtatgccg tgctcttcgt agcgggcgcc gttccagctc
     4861 tccataaaga aacctcgggc gtctccgaag acatcaggct cgatgacgag tactcccgac
     4921 aattccgtcc tgatgacgtt caaatgacgt attcctcgct cttcaggatc tcgagcaggt
     4981 accggccgta gtcgttcttc ctcatctggt gggcgaggcg ttcgagccgt tcggagtcga
     5041 tgtagccttt gcggtaggag atctcctcgg gacaccctat cttgaggccc tgccgctgct
     5101 cgatggtctc gatgaagttg gatgactggt gcagcgactc gtgcgtaccg gcgtcgagcc
     5161 acgccatccc gcggcccatg agctcgaccc gcaactcccc gcgctccagg tagtcgacgt
     5221 tgacgtcggt tatctccagc tcgcctctcg ttgacggcct gaggtcggca gccacgtcca
     5281 ataccctgtt atcgtagaag tagaggcctg tcacggcgta gtgcgaacgg ggcttctccg
     5341 gcttctcctc gatactgaga accgtcccat cgccgtcgaa ctctacgacg ccgtagcgcc
     5401 ccgggtccct gacgtagtac ccgaagacgg tggcccctct ctgcctgcct acggatcgct
     5461 caaggagctc ggagaaacca tgcccgaaga agatgttgtc cccgaggacg agcgagacgc
     5521 tctcggaccc cacaaactcc cgcccgatga cgaaagcctg ggccaggcct tctggcctgg
     5581 gctgcacggc gtagctgaaa tctacacccc actgcgaacc gtcgccgagc agatcctcga
     5641 agcgggatag gtcttcgggg gtggagatca ccagtatgtc cctgatgcca gcgagcatca
     5701 gcgtggacaa tggatagtag atcatcggct tgtcgtagac gggcaggagc tgcttgctta
     5761 ccgccctcgt gagcggatac agccgcgtac ccgagccccc tgcgagcacg atccccttca
     5821 cgccggcccg cccaacccga gccgctctcc cctgtagccc cgcgagagcg cggcttcgca
     5881 ccaccagcgg ttctccaggt accagcgcac ggtcttctcc atgcccgtct ccagcgtctc
     5941 ctctggcctc cagccgagct cgcgttctat cttccccgcg tctatggcat agcgtctgtc
     6001 gtggcccggc ctatccgcga cgaaggtgat gagcgattcg tgcggcgatc cgggaacgag
     6061 ctcgtcgagc agggcgcaga tggtcttgac gacctcgagg ttcgtccgct cgttgtggcc
     6121 gccgacgttg tacgtctcgc cgggacggcc acgctggagt acagcgagga tagccctaca
     6181 gtggtcctct acgtagagcc agtccctgac ctgctgcccg tcgccgtaga ctggcagggg
     6241 ttctcctcgc agggcggtca ggatcatgag cggtatgagc ttctcgggga agtggtaggg
     6301 cccgtagttg ttcgaacagt tggtggtgag cgtggggagc ccataagtgt ggtggtagac
     6361 ccgcacgaaa tggtcggagg ccgccttgga ggcggcgtag ggggagctgg gcctgaaagg
     6421 gctctcctcg gtaaagtagc cctcagtgcc caacgtgccg tacacctcgt ccgtggagat
     6481 atgcagaaag cggaaggcgc cgtggtcctc cccttggagg gcgctccagt aggaaagcgc
     6541 ggcctcgagg agttcgtggg tctcgaccac gttggtctgt acaaaggcct tcggcccgtc
     6601 gatggagcgg tcgacgtgcg tctcggcggc gaagttgacg acggccgagg gacgatactc
     6661 agcgagcagg cggtcgacta gcccgcggtc ccctatcctc ccctttacga agacgtggtc
     6721 ggggttatcc gagaccgacg caagcgtgtc ggggttgccg gcgtaggtca gggcgtcgag
     6781 attgaccacc ctatagtctc cgaccgcacc aaggacgaag ttgcccccta taaagccggc
     6841 gccgccggtg acgaggatgg tttgtttctc tcgctgcaat gcgctcctct gtcttggttg
     6901 agggctgatc gccaatagct ttccagcctg tagcctacta gacgggtcgt ggggtcgcca
     6961 tgttcttgca accccgcgca ggtgcgtgat acttttctta cgctgtggtc tctggcttct
     7021 acactaccgt tcgcggctag attccgggtc cgtaaaatcg attcggtccg atttccaacg
     7081 aaaggctagg cttgtattga gcgttcaggc gcgggtgtcg agacagggtg ttatccggag
     7141 gtaccgcggg tggctgccag agattccgga tgatgccata gccacgatag gcgaggggga
     7201 tacgcctctg atcgaggcca agaggatctc tgagcgcgtc ggcgcccgcc tgttcctcaa
     7261 gttcgagggt atgaacccga cggcctcgtt caaggatcgt ggcatgaccg tggccatgag
     7321 cagggcagcc gcaacgggga gccgcgcctg cgtctgcgcc tcgacgggca acacagcggc
     7381 ctcggccgcg gcctacgcgg cgcgtgccgg catggagtgt ttcgtcgtcg ttccggcagg
     7441 caagatcgcg ttgggcaagg ctgtacaggt cctcgctcac ggggcgcgga tagtccagat
     7501 cgagggcaac ttcgacgtgg ccctgaagct ctccaggagg atcgccgagg acctcggcga
     7561 cgtcaccatg gtcaactcgg tgaaccccga ccgtatagag ggccagaaga ccgcggcctt
     7621 cgaggtcgcg gaagccctcg gagatccgcc cgacgcgctg gccttgcctg tggggaacgc
     7681 aggcaacatc acctcgtact ggaagggctt ctgtgagtgg cgcgggacgg ggcacgctgg
     7741 ctcggctccc cgcatgctcg gcttccaggc tgaaggcgcc tccccgctgg tggctggcca
     7801 cgacttcgag aacccggaga cggttgctag cgccatcagg ataggttcac ccgcgagcaa
     7861 agagggggcg ctctcggcag cgcgcgagtc cgggggcctc atagagagcg ttacggacgg
     7921 ggagatcctg gccgcccagg agatgctggc ccgcgaggag ggtgtcttct gcgagcccgc
     7981 ctcggcggcc ggcatagcag gactcttgaa gctcgcttcc gaaggcaggg ggcctgaagg
     8041 cacggtcgtg agtgttctga ccgggcatgg gctcaaggac cctgatgtga tccttggtcg
     8101 cgtgcaactc cctgaccccg tcccagcgac gttcgaggcg gtcgccggac gaatggagag
     8161 gtaggcgcgg tgatctccgt ccgcgttccc gccacctccg cgaacctggg accgggctac
     8221 gacgcggtgg gtctcgcgct ctcattgagc atgaggatag ggttggacag gtcgcctcat
     8281 cccgagatag atataaaagg gacgggtgcc gacctcatcc cgcgcgggcc cgagcaccct
     8341 gcctacaggg cggcccgctt cgtggccgag ctggtgggcg agtcagacgc ccaattccgt
     8401 ctcgtgcagg agaactctat ccctccgact cgaggcctgg gcgggagtgc tgccgcgctc
     8461 gttggcgggg ccgtcgccgc caacgacctg ttcgggggtc agatcgccgc gcccgaccta
     8521 ctcaacatcg tctgcgagct cgatggccac ccggacaacg cagcccccgc gctcctcgga
     8581 gggctcgtga tcggaaccct caccccggta ggcgtgagcg ccgtgcgcct cgaaccgaag
     8641 gacctcaagg tggtcgtcgc cgtccccgac ttcgccgtct ccaccactgc agcccgccgc
     8701 gccctccctg actcggtccc ccaccgcgac gccgcattca acgtcggccg ctccggtcta
     8761 ctgctcgggg cactcgcgac aggcgagtac cacctcctgc gcgtcgccat gcaggacagg
     8821 ctccaccaac cctaccgctc ccacctcatc ccaggactgg aggacgttat agaggccgcg
     8881 ctaaagacgg gcgcccatgg cgcatgcctc tccggctcgg gccctaccgt tctcgccttc
     8941 gcacccgaga ccagagccag ggacatagcc tcatcgatga aaacaacctt cgaggcgcga
     9001 ggtgtcgggg tagagtcctg ggtgctcgac gttgacctcg caggggcccg cgccgagcca
     9061 ccggaagaag tcaccaccct gagtccggtg cccgtactct agagcggccc aagaaagttg
     9121 caaccccaac gtcagccgca ggcttggccc tcctcaggcg gctgcctaca gagctcctcc
     9181 tgggagatgg cctatgtgcc ggccagcgcg gcgcacacgg cgagcccctc cgcacgcctc
     9241 agcgcctcga agacgccggg ctcaccatcc gtcggggtga gctgcgtgtt gaccgacacc
     9301 gtcaccgagc ggctcccgtt gggacttgcc gccatgaact gggtgtagcc gggggtgttg
     9361 cccgtgtgac cccacaccgt cccgcaatgc gtctcgtagc ggaagatgcc cagtccggcc
     9421 gagttcttcc caggaccggg cggttcggag cttcctccct caacgacccg tctctgcttt
     9481 gcccgcgtcc gttcgtcgaa gagctcaccg ccgacgtaac cgcggatgaa gtcgttgagg
     9541 tcggctggcg tggacactat gccccctgaa gcccaggccc acccggcagc caccacctcg
     9601 ctcaggtctt cgggccgctc ttgggagggg tcgttgtcgt agccgtggat gaagggcttc
     9661 cttaggttgg ggccgcgggg tagggtagtc tttctcagcc caagagggct gtagacccgc
     9721 tcccggagct ggtcctcgta actcataccg gtggccgcct cgaccatcag ggcgacgacg
     9781 atgttgtcgg agtttgagta tttgtactcg gaaccgggat cgaagagcag atcctcgccc
     9841 tccacgaagg agagtagctc ctcaggcggg ggggctttct tgaggctcgc cagcagcgcc
     9901 tcttggaagt ccgggtccag cgagaagtcc gggaccccgc tggtgtgatt gagtagctgc
     9961 ctgagcgtca ccttggacca agccttcggc aactcccgca aacgctcgcc gatcgtgtcg
    10021 tggagcgaca acgtgccctt gctcaccagc gagagcgcca cggccccgct gaaggccttc
    10081 gcggcgctcg cgatgcgcat gtggtccttg gcgcgcaggg gcagatcgcc attcaggtta
    10141 cggaccccga aggaatggac ctccttgtgt gggcctcgtt gcacgatggc gataacacct
    10201 ggcggacctc cgcgcatggc gaccagttcc tcgagcgcgc gatccagcgc agcgtccgcg
    10261 cctttggcac cttctttccc gaaagcaacg gtcttgccag ccaggtaagg aacggagacc
    10321 accgcagtgg ccagggccgt ctgtttgagg aaccgcctcc tgtcgatgat cgtcgctcct
    10381 ccggatggcc cttgcgtact tgcgtttttt gccattctcg tcttcctttc ctcccacgct
    10441 gaactaggca gggagtatcc gctgaggcgg ggcgactgtc aaatgaatag aaagatggcg
    10501 ggctgcgccg ccagggcatt tgtgatgtcg acagtgtgct ccgcagcatt ttcttcgtgt
    10561 gtgcgggagt ggagatatgt gcaccttgac aaccctctac cgttgtgtca cccttcatcc
    10621 aacttgccgt cgagtgagtg aggaatgttg agcgaggttt cggaaacggc gcgaaggaag
    10681 aagatctcgg gcttgatgct caagatctcg cgcctgctcg ctccgcccat ccacccccga
    10741 gtatccacgg tcgccgtacc gtccgcccta cgactaatta gcctggcggg ccgggacggc
    10801 gttctccggt ccgcctagag ccgcaagacg ggcacatccc gttcttcggc aaggttggga
    10861 caagatccat tatttagaac ggggtaaacc gtggaaaaca cagctttcaa catcgaatac
    10921 aagccgcgta cgggtagcga ggcgatgtgc gaggccctac tcgacgaagg ggtcgagtat
    10981 ctctttggat accctggggg tgcgatcatg cctttctatg acgccctccc cgattacccg
    11041 ctgaaacacg tcctaacccg ccacgaacag gcggcggccc atgcggcgga cggctacgcc
    11101 agggcgacgg gcagggtcgg ggtgtgcgtg gccacgagtg gtccaggggc gaccaacctg
    11161 gttacggggc tggccacggc ccagatggac tccgtgccgc tggtggcgat cacgggccag
    11221 gtcggacggc cggcgatggg caaggactcc ttccaggaga cgaacgtgat gggcatgacg
    11281 ctgcccttta ccaagcactc cttcctggtc gaggaccctg atgagctcta cccgacgatg
    11341 cgacgggcct tcgagatcgc ccgcagcggc aggccaggac ccgtcctcgt tgacgtgccc
    11401 aaggacgtgc agttcggggc cacaaacgac aacggctacc gtcgtctgcg ggaggcccca
    11461 cctcccgtcg agtcgcccgg gctagagcgg gcagcgatgc tactcaggag cgcgaagcgg
    11521 cccctgatcc tggccggaca cggcgtcacg ctggggggcg cagagatgga gctcaggctg
    11581 ctcgctgaga ggacgggcat acccgtcgtg acgacgctgc ttggcataag cgccttcccc
    11641 gacgaccacc cgctgtatct tggctggcca gggatgcacg ggcaggccag cgtcaaccgt
    11701 gccatagacc gctccgacct cctgttcgcc gtcgggatgc gcttcgacga caggatcacg
    11761 ggcgcggccc acaagttcgc gcctggcgcg aagatcatcc acatcgacat cgacccctca
    11821 gagctgggca aggtcataaa gcctaccgtg gggatagcag cagacgcgaa ggccgcgatg
    11881 cgttcgatcc tcggacacct ggataacgcc cctcacgagg gcggaggatg caagcagtgg
    11941 cgcgaggaac tggcgaagag ccaggaaccg gagaggcgcc gcaaggagtc ggcgcgggac
    12001 gagtacggtc ctatacaggt gatggaagcc ataaaggggg cggcgggctc cgacctgcgg
    12061 ctcgttacag acgtggggca gcaccagatg tgggcggccc agttcttcga gttcaccaag
    12121 tccaacagcc acatcacgag cggtggcctc ggcacgatgg gcttcgcgct gcccgcagcg
    12181 atgggcgtgg ctttcgccta tccggaagac ccggtgtggg tcatagccgg cgacggtggc
    12241 atccagatga acatccagga gctcgccacg ataagggact tcgggctgaa cataaaggtc
    12301 gccgttctga acaacggtta cctggggatg gtccgccagt ggcagcagtt cttccacaac
    12361 cgccgctact cggagacccc cataacgggg cctgactacg agcagctcac cgccgcctac
    12421 ggggtcgctg gacgcaccgt cagggagggc gacgacgtcg aagcggccgt gaggtgggcg
    12481 caagagcagg atggctgcac tatcctggac ttccacatcg accccgaggc gaacgtctac
    12541 cccatgatcc catcaggcat gtccgtcaac gagatgatcg aggaagacgg tgcctgagtc
    12601 ggcaacggga gtcgccctga acaccatcga ggtccgcttc acgggcggta tgctcgccct
    12661 gaacaggcta ctcatgacgc tgcagaacaa gaggatgccc gtcgcaggct tcacgctagg
    12721 gagcgacagt gacggaatgc gggccaccgt cctgctcgac tgcccacccg agtcggcgct
    12781 gcgctacacg gccctcatct cggccctcga agacgtaagg gaggcgggac cggctgagac
    12841 catagaggta gccctgatcg agacctcgag agactggcgc gagcttgcgg agcaatgggg
    12901 cgtcgaagcg cacgaagacg atggaacggt cgtagcgtcc ggtgagcccg acaaggtgga
    12961 ggcctttctc gccgctgtcg gcgtggaaga cgcggttcgg gttggtcctc tggcccgtcc
    13021 ggaatcgggg ggaggaatct agatggcacg ggtataccgt gagggagata tagggaatga
    13081 gatcaccacc aggagagtgg cgatacttgg atacggcagc cagggccatg cccacgccct
    13141 gaacctgaag gagtcaggct gcgacgtgag ggtcgggctc tacgaaggtt cgaagagctg
    13201 gtccaaagca gaagaggacg gcctgcaggt gaagaccatc gctgaggcgg taagagaagc
    13261 cgacgtggcg atgatgctcc tccccgatga gaagcagccc gccgtcttcg aggccgaggt
    13321 cgggccgaac ctctcagagg gcgacctcat gctcttcgcc cacggcttca acatccactt
    13381 caaccagatc gtcgtccccc ccgaggtcga cttgggcctc gtcgccccca agggaccagg
    13441 acacgtgctc aggcgtcttt atgtcgaggg caaagggatg ccctcgctct tcgcggttca
    13501 gaacgacgct tctgggcagg ctcgggatgt gatcctctcc tacgcccgcg ccatagggag
    13561 cggccgcgcc ggcatcatcg agaccacgtt cgctgaagag accgagacgg atctcttcgg
    13621 ggagcaggcg gtgttgtgtg gggggcttac ggccctcctc acggccgggt tcgagacgct
    13681 cgtcgaggcg ggctaccagc ccgagctcgc ctattatgag tgcgtaaacg agctcaaact
    13741 catcgtcgac ctgatctacg agggcgggct tgcggggatg cgctactcga tctcgaacac
    13801 cgccgagtac ggcgactata cggcagggcc gaagatcatc gacgaggccg ccagagagag
    13861 gatgcgttcc atcctcgcgg atatccagag cggcaagtgg gccaaagact gggtgctgga
    13921 gaaccaggcc ggggggtcca gcttcctcgc gatgcgccgc agggaggccg agtcgcaggt
    13981 ggagaaggtc ggacaggagc tgcgggcgct cgcggccgag ggtatcgaga cgcccgctgg
    14041 ctccgctgga ggtgagaacc tatgagtcgt aaagctt
//
LOCUS       DASTKM010000033         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_5290, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000033 DASTKM010000000
VERSION     DASTKM010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5290"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFQ10_01280"
     CDS             <1..384
                     /locus_tag="VFQ10_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964000.1"
                     /translation="ACGGGSTGYDYETASHHDPAELEPIKGTDVNRVIIDAEGVERAG
                     IQTAPIRQNGQGTVMPHSAVIFDAEGHTYTYTSPEPRTYVRQKIVIDDVVGDSVMLSD
                     GPPAGTEVVTVGAAMVYGSEFEVAH"
     gene            401..3511
                     /locus_tag="VFQ10_01285"
     CDS             401..3511
                     /locus_tag="VFQ10_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9964001.1"
                     /translation="MRWIVGSSLRFRWLVIFAAVALMVFGIGETRNADVDVFPEFAPP
                     QVEIQTIAIGNSSEQVEELITVPLEDQLNGIPGLEEMRSKSVADLSQIQLIFSRDVDL
                     YESRQLVQERLTVAAPTLPTWASPPWMMPPLSSTARVMKIGITSDELNMEEMSMVAYW
                     KIRQELLRVDGVANVMIYGEQLQQQQVYVDPSKLAEHGVSLEQVMDQTADSLDAGLLR
                     YSEGAVIGTGGFVQEDGQRLDVRNVLPIVTPDDLAKIPLEGSNGTNVRLDDVASVGWG
                     HQPLIGDAVIDDGRGIMLIVQKFPNANTLEVTRGIEETVDDMRPGLPAMELDTTIFRP
                     ATFIEQAFGNLTRALLLGVLLVVLILAAFLFEWRTAFISLIAIPLSLLAAILVLQAFG
                     AVINVMILAGLVVAIGVVVDDAIIDVENVVRRLRQERSEGTGRSMVAIVLDASVEVRT
                     AITYATLINVIAVVPVFFLTGLSGAFFKPLVLSYGIAVLVSMIVALTVTPALCLILLS
                     RGHRHQESPLLRVLKRGYGAVLARVVRTPAPAIVTVAVVMLAGVVVAPTLGTILLPNF
                     KERDFLMHWLAKPGTSQPEEFRVSVRACKDLQEIPGVRNCGSHIGQAFQSDEPYGVDF
                     GENWVSINPAVDYDKTLASVSNVVDAYPGLYRDVQTYLRERVREVLTGTSEAIVVRIY
                     GPDLDVLYAKADEISHKMQGIPGLIDAHADLQEDLPHTEIEVDLAAAQAHGLKPGDVR
                     RQASTLVASEEVGDIFRGGKAYDVHVWSTPETRNSVSSVRELPIDTPSGEQITLEQVA
                     DVRLAPTPNAIERVNQSRRIDVGANVEGRDLGSAVSAVEERIAGVELPQEYRMEVLGE
                     SSELAAAQKSLLIYGGAVALAILFLLQASFGSMRLALLLFLTLPMALVGGVLAVWLGD
                     GILSLGSLIGFLTVFGIAARNGILMISHFQHLEHEEGEPFGPGLVLRGAKERLAPILM
                     TAGATGLALVPLAVVGSVAGHEIEHPMAIVILGGLVTSTLLNLFVLPSLYLRFGKSKR
                     ALSSSS"
BASE COUNT          728 a         1233 c         1302 g          790 t
ORIGIN      
        1 gcgtgtggcg ggggaagtac gggctatgac tacgagacgg cgagccatca cgatcccgcc
       61 gagctcgagc cgatcaaggg aacggacgtc aaccgcgtaa taatcgacgc cgaaggggtg
      121 gagcgagccg gcatccagac ggccccgatt cgccagaacg gccagggaac ggtcatgccc
      181 catagcgccg tcatcttcga cgccgagggc catacgtaca cttacacctc tcccgagccg
      241 cggacttatg tgcggcagaa gatcgttatc gacgacgtcg tcggtgatag cgtcatgctc
      301 tccgacggcc cacccgccgg tacggaggtt gtgacggtcg gggccgctat ggtgtacggc
      361 tccgagttcg aggtcgcaca ttgaaaggta gggattgctg atgcgttgga tcgttggatc
      421 gagcttgcgg ttccggtggc tggtaatctt cgccgccgtg gcgctgatgg tcttcggcat
      481 cggtgagact cgcaatgcgg atgtagacgt cttccccgag ttcgctccgc cacaggtgga
      541 gatccagacc atcgcgatcg gcaactcctc agagcaggtc gaggagctga tcaccgtccc
      601 gcttgaggac cagcttaacg ggatccccgg gctcgaggag atgcgctcca agtcggtcgc
      661 cgacctatcg cagattcagc tcatcttcag cagggacgtc gacctgtacg agtcgcggca
      721 attggtgcag gagcgcctga cggtggcggc acccactttg cctacctggg cgagcccgcc
      781 ttggatgatg cctccgctgt cgtcgacggc ccgggtcatg aagatcggga tcacctcaga
      841 tgagctgaac atggaagaga tgtccatggt cgcctactgg aagatcaggc aggagttgct
      901 gcgcgtggac ggcgtggcga acgtgatgat ctacggtgag caactccaac agcagcaggt
      961 gtacgtggat ccgagtaagc tcgccgagca cggagtctcg ctggagcagg tgatggacca
     1021 gacggctgac tccctggatg ccgggctttt gcgctactcg gaaggcgcgg tgatcgggac
     1081 cggcggtttc gtccaggaag atggccagag gctcgacgtg cgcaacgtgt tgcccatcgt
     1141 cacaccggac gacctcgcca agataccgct cgagggaagt aacggtacga acgtgcgcct
     1201 cgacgacgtg gcgagcgtag gctggggtca ccaaccgcta atcggtgacg ccgtcatcga
     1261 tgatggccgc ggcataatgc tgatcgttca gaagttccca aacgccaaca cgctcgaggt
     1321 gacgcggggc atcgaagaga cggtcgacga catgcgccct ggccttcctg ccatggaact
     1381 cgacacgacg atcttcaggc cggccacctt catcgagcag gcgttcggca acctcacgag
     1441 ggcgctgctg ctcggcgttt tgctcgtggt cctgatcctg gccgccttcc tcttcgagtg
     1501 gcgcaccgct ttcatcagcc tgatcgcgat cccgctgtct ctgctcgcgg ctattctcgt
     1561 gctccaagcg ttcggcgccg ttatcaacgt gatgatcctg gcggggctcg tcgttgccat
     1621 aggggtcgtc gtcgacgacg cgatcatcga cgtggagaac gtcgtgcgac gattgcgcca
     1681 agaacggagc gaaggtaccg gcaggtcgat ggtcgccatc gtgctcgatg catcggttga
     1741 ggtgcgaacg gcgatcacgt acgcgacgct gatcaacgtc attgcggtcg tgccggtctt
     1801 tttcttgacc ggcctctccg gtgctttctt caagccactt gtgctctcct acgggattgc
     1861 ggtgctcgtc tcgatgatag tggcgctgac ggtcacgccg gcgctgtgcc tgatcctttt
     1921 gtcgaggggg cacaggcacc aggaatcccc cctgctacgg gtgctcaagc gcggctacgg
     1981 cgcggttctc gcgcgtgtcg tccgcacccc ggctcccgcg atcgttaccg tggccgtcgt
     2041 catgctggcc ggcgttgtgg tggcgccaac gctcgggacg atcctgcttc cgaacttcaa
     2101 ggagagggac ttcctcatgc actggttggc gaagccaggc acttcccaac cggaagagtt
     2161 tcgggtctca gttcgggcct gtaaggacct gcaagagatc cccggcgtgc gcaactgcgg
     2221 atcgcacata gggcaggctt tccaatccga cgagccctac ggcgtggact tcggcgagaa
     2281 ctgggtcagc atcaatcccg cggtcgacta tgacaagact ctggcctccg tgtcgaacgt
     2341 agtcgatgcc tacccggggc tctatagaga cgtccagacg tacctgagag agagggtcag
     2401 ggaggtgctg acgggcacga gcgaagctat cgtcgtgcgc atctatggcc ccgacctcga
     2461 tgtgctctat gccaaggcgg atgagatctc acataagatg cagggcatac ccggcctcat
     2521 cgacgcgcac gccgacctcc aggaggacct accacacacg gagatcgagg tagacctcgc
     2581 tgcggcgcaa gcccacggcc tcaagccggg cgacgtgcgc cggcaggcat ccacactggt
     2641 tgccagcgag gaagtcggcg atatcttcag gggcggcaag gcctacgacg tgcacgtgtg
     2701 gagcacgccc gagacccgca acagcgtgag cagcgtgcgc gagcttccga tcgacacgcc
     2761 ctcaggcgag cagattacac tggagcaggt ggcggacgtt cgcctcgcgc cgactccaaa
     2821 cgccatagag cgcgtgaatc agtcgcgccg gatcgacgtc ggcgccaacg tcgagggacg
     2881 agatctcggc tccgccgtga gcgccgtcga ggagcgcatt gcgggagtcg agttgccgca
     2941 ggagtaccgc atggaggtgc tgggtgagtc cagcgagctg gccgctgcgc agaagagcct
     3001 gctgatctac gggggcgccg tggcgctcgc gatcttgttc cttctgcagg cttcgttcgg
     3061 gagtatgcgt ctggcgctcc tcctcttcct taccttgccg atggcgctgg tgggcggggt
     3121 tctcgcggtg tggctgggag acggcatact gtcgctcggc tcgctgatcg ggttcctgac
     3181 tgtgttcggc atcgcggcta gaaacgggat cctgatgatc agccacttcc agcacctcga
     3241 gcacgaagag ggcgagccgt tcgggcctgg tctcgtgctt cgcggggcga aggagcggct
     3301 cgcgccgatc ctgatgaccg ccggggctac ggggttggca ctcgtcccgc tggcagtagt
     3361 gggcagcgtg gccggtcatg agatcgagca cccgatggcg atagtcattc tgggcgggct
     3421 cgtcacctcg acgctgctga acctgttcgt cttaccctct ctatacctgc gcttcgggaa
     3481 gagcaagaga gcgctctcga gctccagcta accaccactg gagcctgctc ggccgcgccg
     3541 gctcaggtag gagtgttggc cgatggtatc aacaatgccg gggacctact ctggggtccc
     3601 cggctttgct tggaaaggga ctttgcgtaa gcctaccccg atgctgcgat gagccacgag
     3661 acgataccgg cgatcacaac gagaatcgag cattgggcca ctagctttcc aattgccagc
     3721 cgtgagctct ccgaatagga catctgctcg cccaccttct tgatcgtcct ctcatgcgag
     3781 acaatgccaa ttcccgcgat ccagctcccc gctctgccag cttggcgccc ggcatacgcg
     3841 gttggctgac gtgcacggtc gcctccccaa tccagaatat atcgcccacg cccacctcgg
     3901 cttcgataac tcccgagacg gtgaggtcgc tccggcatct ccttgagggg gacttgttcc
     3961 actgacctca tatacttctc ctgtgtaggt ggtgtcggag agtaaagtag tgtgctagcc
     4021 gttcctgggt ctgcccatct tgattcactc tgt
//
LOCUS       DASTKM010000034        17207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_533711, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000034 DASTKM010000000
VERSION     DASTKM010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 17207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17207
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533711"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /gene="ftsZ"
                     /locus_tag="VFQ10_01290"
     CDS             <1..1075
                     /gene="ftsZ"
                     /locus_tag="VFQ10_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET9964002.1"
                     /translation="GMLDAATNYLAVIKVVGIGGGGTNAVNRMINSGLQGVEFIAINT
                     DAQALQMCDADQKIHIGEKITRGLGAGADPKIGMEAAEENKAEIEEAMRGADMVFVTA
                     GKGGGTGTGAAPVVAKIARDSGALTVGVVTRPFAFEGRRRSTYAEEGIKRLKENVDSL
                     IIIPNDRLLQVAEKRTSMMDAFKMADDILRKGVQGITDLITVPGLINLDFADVRTIMH
                     DSGSALMGIGESSSETRGTEAAKTAISSPLLEASIDGATGIILNITGGPDLGLFEVNE
                     AAEIVHNAAHQDANLIFGAVIDENNGERVSVTVIATGFDQRLANQRRSERPAPEPLQQ
                     EEPAAASQETGDVLDIPAFLRRR"
     gene            1090..1851
                     /locus_tag="VFQ10_01295"
     CDS             1090..1851
                     /locus_tag="VFQ10_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HET9964003.1"
                     /translation="MRRADTQTAFGAPSTMHIARGGAVYVAPDTAPKGAGIFFFARLG
                     GTSEPPYDSLNVSKKVGDDPRAVDENLSVIGSAMDLRPAAWVRQVAGDSVVRVCEPGF
                     AGEADALVTAETDLCLSVAVADCVPVALVGEREVGMVHSGWRGTLSGLSGKAANSLGG
                     DLVQAYIGPSIRRCCYEVSAELAAQFAAEFGDEVVSGRHLSLQDAIRVDLERAGVGVN
                     DLGLCSGCRPDLFYSHRKQGPLTGRNLASVVREAR"
     gene            1848..2570
                     /locus_tag="VFQ10_01300"
     CDS             1848..2570
                     /locus_tag="VFQ10_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET9964004.1"
                     /translation="MSVHVTEIRDRLAGVHEKIQRALERSGRGEEQVRILVATKYYDP
                     EHLSALTQAGVDLIGENRAEDLLEKQALFGDRFEWHFIGHLQRRKAKVVVPRVTLVHS
                     VDSVRLIEELAKRAPEGGADILIQVNVADEESKYGVTEEEVDELLDVAAQTGGRVRAR
                     GFMTLAPLVDDPEDVRHVFAKLRTIRDSLRKSWSPHFDLSELSMGMSGDYEVAVEEGA
                     TVVRIGRMLIEEDGPVRRHDGS"
     gene            2560..3132
                     /locus_tag="VFQ10_01305"
     CDS             2560..3132
                     /locus_tag="VFQ10_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET9964005.1"
                     /translation="MGVRDRLDRIAAWFGFGVDDEDYYEDEVEEERRYAGEAYAGGAR
                     RGAEPGPTVRRLGRAERSSAFGTSLGDLFGSEGSGRGESYQNPPSHLRPVPDQRTTRV
                     SVLEPSSFNDAQNLADRFKRQQPVILNLQNVDGDLSRRMVDFCSGLTYALDGNIQSVA
                     NRVFLLTPRDVEVSAEERKRIAERAFFNQL"
     gene            3149..3931
                     /gene="proC"
                     /locus_tag="VFQ10_01310"
     CDS             3149..3931
                     /gene="proC"
                     /locus_tag="VFQ10_01310"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET9964006.1"
                     /translation="MKKVGIIGAGKIGGSLLYRLAESGGFDLGASDIHPEQLRPYESI
                     GVRTTSSNRELAAASDLVIAAVKPWDVAQVMEEISPTFRDNNKAVTSVAAGVSIERIS
                     ESLPQGTAILRVMPNVCASVGLGSAVVTSNEAGRAALPTVMDIFRHVGDVLELPERLF
                     DAATALHGSGPAYVALFADALIQAGVREGIPRDVARRLVIGTIGGTAVLLKERSAHQV
                     RDEVMTPGGTTAAAFVAMEKAGFVAAVYDGVEAATERARRLG"
     gene            3931..4272
                     /locus_tag="VFQ10_01315"
     CDS             3931..4272
                     /locus_tag="VFQ10_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET9964007.1"
                     /translation="MSLLLLQQFGFSVASLLAGVVNYTYYILFGFIIASILFSWFPGY
                     PSSTFMQAIYDAVKAVTNPILWPIRSRVPMLQLGGFGIDLSPIIAILGLSIARSLLLL
                     VIENFIAPVTG"
     gene            4279..5274
                     /locus_tag="VFQ10_01320"
     CDS             4279..5274
                     /locus_tag="VFQ10_01320"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET9964008.1"
                     /translation="MPIRPIDVRRKEFKSSFRGYDANQVDDFLDAVADEFERNYTENQ
                     RMREEVSSLRDRLQQFEDLEGSIQAALVHAEQASNDLRRAAAQEAEGIKQSAQREADF
                     TIREAQSRSHQMLADSSARIERVQDSYDALQEAKRSFANEFRHLLKTYTDIMENMEVA
                     SAREIEASLRGRLDTESIAVVREAAQEEARSAEEAMVTAEAMAAAGMVPEPVSEDAQD
                     TLSGLEAVDPADQELEPAMDEQPTEVIRPEGSEPETEDPEVAEPEVEPSTAQDEMDQP
                     VQEPPVSVPEAASEVEREEPSLAEESSSQQFFDRQEGERSDSRIYRASHFLRRRE"
     gene            5267..6187
                     /locus_tag="VFQ10_01325"
     CDS             5267..6187
                     /locus_tag="VFQ10_01325"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9964009.1"
                     /translation="MSKGFVVHTSEGANFRVDPAEAGERVDMLVARRLGISRSLVQRL
                     IAENALLVDGMDTSPSFKVRWGQRVEARVPVEGISSEEIPVPVVFEDEHILVVDKPAG
                     LVVHPGAGNLSGTLVNALLNRGIEGGDDPLRPGVVHRLDRDTSGLMILAKGEPAYSTL
                     IGMMSARRVGRHYRTIVEGEGLPRTGTIDSPVGRDPDNPTLMVAGVGKPAVTHFEALA
                     GAAGYTMLRVRLETGRTHQIRVHLSAIGHPVYGDPLYGKPVPGHRLWLHAERLYFEHP
                     VGGEALEFHSEIPQDLRDSAIGLDGSLGLW"
     gene            6247..6801
                     /gene="pyrR"
                     /locus_tag="VFQ10_01330"
     CDS             6247..6801
                     /gene="pyrR"
                     /locus_tag="VFQ10_01330"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET9964010.1"
                     /translation="MSRTSVYEHAHSRVLAPDGLNRSLRRISHEILERNAACLDGLAL
                     VGVLTRGVPLARRISANVRQFEGLELPVGSLDITLHRDDLEKVDPEVRGSSVPFDVTG
                     KTVILVDDVLYTGRTARAAMDALLELGRPAAIRLAILVDRGHRELPIRADYVGKNVPT
                     ARDERVLVNLEETDGEDGVIVVGN"
     gene            6791..7708
                     /locus_tag="VFQ10_01335"
     CDS             6791..7708
                     /locus_tag="VFQ10_01335"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET9964011.1"
                     /translation="METRSFLTLEDIGHEDIREILDLARAFAEGRVRDALEGKTVCLA
                     FFEASTRTAVSFELAARRSGAHVISLSAKGSAIAKGESLIDTVVTLDRLGADAIVLRH
                     QAAGAAALASRHTSAPVINAGDGCGQHPTQALLDLYALCEALGGFEGLRGRRAAIVGD
                     VLHSRVARSVIPGFLQAGMEVALVAPRTLLPPDAGALDLPVLSSVDEALEWDADVLYM
                     LRLQKERMTGARIPSVAEYSRFYGVSRRHLQSGVLVMHPGPVNRGVEISGDVVLDASS
                     LIPDQVSAGVCVRSAVLALSTGAAQSVAA"
     gene            7708..9015
                     /locus_tag="VFQ10_01340"
     CDS             7708..9015
                     /locus_tag="VFQ10_01340"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET9964012.1"
                     /translation="MLGESNSAELVIRHAHIFDPSTGLDGITDVRLSSGRIAEVGERL
                     RGQRELDADGLHLFPGFVDVHAHWRTPGREDEEDIQSGSAAAAAGGFTGVVMMPNTDP
                     IVDRPVVVSGLVSRVERESRVRARVSAALHVGLAGERLTEMRLLKQAGAFCVSDDGLG
                     TASAGVLRNGMLYARSARLPVILHCEDHTLATGVVHDGVAASLAGLPGSPASAEDAAT
                     ATALILAAETGARVHITHVSTALSAALVGFFRGKADVTADTTPHHLTLTEDLVSTLDG
                     LYRVNPPLRPELDRAGVELAVRDGVLDFVATDHAPHASEEKELPIEEAAPGFLGHETA
                     FAALYTDLVLGGRLSLKRLVEVMSCAPGRWVGAGGTLAVGAPADLTLIDLSEEWTVGR
                     GTLLSRSSNSPYLGRTLKGRVVSTIVGGEPVHERTGAKFGARA"
     gene            9002..10129
                     /gene="carA"
                     /locus_tag="VFQ10_01345"
     CDS             9002..10129
                     /gene="carA"
                     /locus_tag="VFQ10_01345"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET9964013.1"
                     /translation="MLVRNGVQTQDRCRARLVLEDGAAFQGWSFAGNGEVAGEVVFTT
                     SMVGYQETITDPSYRGQILLFTYPLIGNYGVIPGDEESRKVQAAAVLAREYTPHPSNW
                     ASERSLAALLDECGVLGVEGIDTRALTRHLRDRGAMRGVISTSESDRNVLREKAKAHP
                     SMVGLDLASTSSELTEPTQLPALGEERCRVVAIDYGVKGSIYRELRSRGASVLAMPGS
                     TGSEEILAAEPDGLFLSNGPGDPAALGAAVETLRPLLGEVPTFGICLGHQLLGLALGC
                     ETYKLPFGHHGANHPVRNLKTGRIEITSQNHGFAVSETSLSDGVGLTHRNLYDGTVEG
                     IACPGKRAWSVQYHPESSPGPRDSGYLFEEFVNGISEKALA"
     gene            10131..13295
                     /gene="carB"
                     /locus_tag="VFQ10_01350"
     CDS             10131..13295
                     /gene="carB"
                     /locus_tag="VFQ10_01350"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET9964014.1"
                     /translation="MPRRDDISSVLIIGSGPIVIGQAAEFDYSGTQACHALREEGYRV
                     ILVNSNPATIMTDPEVADTTYVEPLTAETVTEILRRERPDALLPTLGGQTALNLAVEL
                     NEAGVLDETGVELLGASIPSIRKAEDRKLFSHAMHRIGLNVPPSRTVRSLAEAEQLAA
                     QIGFPLIIRPSFTLGGKGGATATDHRELRRAVIDGLDASPVGSALVEKSVAGWKEFEL
                     EVMRDLADNVVVICSIENVDPMGVHTGDSITVAPAQTLSDRQYQTLRSASLRIIREIG
                     VSTGGSNIQFAVDPESDEYYVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYSLDE
                     IPNDITRATPASFEPALDYVVTKVPRFTFEKFPGAVPRLTTRMHSVGEVMAIGRTFTE
                     SLMKAMASLEVDAQDVHAGLDEPSPYRVFAIFDALRAGMDISEIFERTSIDPFFIASI
                     ARIVAAEGSISEPFGVDDLRELKRIGLTDETIATASGTSVEVVRGVRRALGVSPTYKS
                     VDTCAGEFPALTPYYYSTYEEEDEVRREENASVVVLGSGPNRIGQGIEFDYACVHASY
                     ALEESGYDAVMVNSNPETVSTDYDTSTRLYFEPLTAEHVLEVIKREEPEGVILQFGGQ
                     SPLKLAHELEEAGVRILGTSPDAIDLAEDRSRFGRLLAELGIPHPRFGTASTPEEARE
                     VARELGYPVVVRPSYVLGGRRMEIVYNDDDLDLYLRTSAGTGPGHPTLIDKFMEDYTE
                     VDVDAVSDGEEVYVGGIMEHVEEAGVHSGDSSCVTPPITIHRALLRRIEDYTRRLALS
                     VGVVGLMNVQFVVRGDDVLVIECNPRASRTVPFISKATGVPLAKIATRVLLGEKLGDM
                     ALRASTNGHFSVKAPVFPFDRFADVDPLLGPEMRSTGEAMGIDRTFGGAFAKALTAAG
                     QELPVSGRVYISVANREKRAVVLIARAFADLGFEISASEGTAEVLKNNGLPVMIVPKI
                     GELGEDVLGLIEAGGVDLIVNTPWGRGPRTDGYLIRRRALMQGVPCITTLAGAAAAVQ
                     GIEARVRGGTSRVNSLQGLYATRA"
     gene            13292..14026
                     /locus_tag="VFQ10_01355"
     CDS             13292..14026
                     /locus_tag="VFQ10_01355"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET9964015.1"
                     /translation="MKFYSVRVEEREELGSYTLLRYPWTDGPVEPGQFVMARAAGYPA
                     TLDPFLARPFSFYDYDGRIASLLFEVRGRGTALLDLASTIEVTGPLGKGFRIDPGLGS
                     AALLGGGIGVVPLKILGRRLDAVGIPHDIYLGFADAKPAAVARDFPGAEVATMDGSEG
                     THGTVLDATDSLEGYGAVYACGPNPMLAAVKRAAGPNSLCQLSVEERMGCGNGSCNGC
                     VVPVRGGGFIRSCIEGPVFHSDVLAW"
     gene            14026..14955
                     /locus_tag="VFQ10_01360"
     CDS             14026..14955
                     /locus_tag="VFQ10_01360"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET9964016.1"
                     /translation="MREQKAGVDLAVELCGLPLRTPLIPASGTLSKEALGEVEDVYGA
                     MLPKTTTPAARAGNPPPRIAETAAGMVNSIGLQNPGVHGFLQGLDAFDLGVPILVSVA
                     GDTVDEFTLLCEILAGDDRVSAIELNLSCPNVECGGLAFCAGPASVEQVVAACRTAVP
                     GKPILAKLTNEGVVENARAAESAGAEGLTVINTLPALTIDAHARKVLVRGGLSGPAIK
                     PIALRAVYDVSAVVNVPVIGSGGVVSGTDVAEFMLAGATAVQVGTASFVRDPHEILDE
                     FTAYLKESGLGAAELKGALRSVRSGMTGGQISG"
     gene            complement(14940..16346)
                     /locus_tag="VFQ10_01365"
     CDS             complement(14940..16346)
                     /locus_tag="VFQ10_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9964017.1"
                     /translation="MVSEGGERKTFALVLLCCASFVAVLDLTIVAIALPSIRRELGIS
                     GGDAQWILTGYALSFGGLLLLMGRAGDLYGRRRLFVSGLALFAAASLLGGLAWAPWVL
                     VAARLLQGVGGAALVPASLALVAATFAEGEERNRAMGVYGAMAGVGFVFGMVLGGVIT
                     QFLGWRWVLLVNVPVALAVLSLAPVAIDESKDDAAPRTLDLAGAATATLGLASLIYAI
                     SEAPKNGWASPATLGTAGMGTVLLGVFVAHERRASTPLVALPILYRRAVAVPNLAVVL
                     KSMVSAAQLYVLTLYFQDALDRTPLEAGLLFIPMTAASVVASPVAGRLTTRLGARRTA
                     VWGFAMSGAGLVLVAWLMPQKDALVAILLGMAVAEAGFVSASVPLTVAATDGVGDNER
                     GLASGVLSTATELGNALGFAAVGAVIAAATATGPVGADALLSGLRWGLWSATGFAVLA
                     LMLVVVSMGSSSRRQPEI"
     gene            16464..17063
                     /locus_tag="VFQ10_01370"
     CDS             16464..17063
                     /locus_tag="VFQ10_01370"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HET9964018.1"
                     /translation="MEQRIPEGEVPEEVRLVNEFLNTLDLETYGEHAGKPDEEREGFR
                     SPERLRAWFVGRGLLGREVAVNEADRQLAVRVRDALRSAALANSVPARERSDAAGWSF
                     QELPLVVRFGENSKPELVSGEGGVRGALGRILADVVVASSKGTWARMKICAAQDCRWA
                     YYDHSKSRTGRWCAMETCGNRHKTRRYRRKRRSSAWQEG"
BASE COUNT         3258 a         5436 c         5665 g         2848 t
ORIGIN      
        1 aggcatgttg gacgcggcga ctaattatct ggcggtcata aaggttgtcg gcattggcgg
       61 agggggaacc aacgccgtga accgcatgat caactccggt ctgcaggggg ttgagttcat
      121 agcgataaac acggatgccc aggctctcca gatgtgtgat gccgaccaga agatccacat
      181 aggggagaag atcacccgcg gcctcggcgc cggcgctgac ccgaagatcg ggatggaggc
      241 cgcagaggag aacaaggcgg agatcgagga agcgatgcgc ggtgccgaca tggttttcgt
      301 cactgcgggc aagggtggcg ggactggcac gggcgccgcc cccgtcgtcg ccaagatagc
      361 cagggactcg ggagcgctga ccgtcggggt tgtcacgcgg cctttcgcgt tcgaggggcg
      421 caggcgttct acatacgcag aggagggcat caagcgcctc aaagagaacg tcgactccct
      481 catcatcatt cccaacgaca ggcttttgca ggtcgccgag aagcgcacca gcatgatgga
      541 cgcgttcaag atggcggacg acatcctgag gaagggtgtc cagggcatca ccgacctcat
      601 tacggtgcct ggcctcatca acctggactt cgccgacgtg cgtacgatca tgcacgactc
      661 gggttcagcc ctcatgggaa tcggcgagtc cagcagcgag actaggggaa ccgaggccgc
      721 gaagaccgcc atctccagcc cgctgctcga ggccagcatc gacggggcga cgggtatcat
      781 actgaatatc acgggtggcc ccgacctagg gctcttcgag gtcaacgagg ctgcggagat
      841 cgtccacaac gcggcgcacc aggacgcgaa cctgatcttc ggcgccgtta tcgacgagaa
      901 caacggtgag agggtgagcg tgaccgtgat agcgacaggc ttcgaccagc gcctcgccaa
      961 ccagcgcagg tccgagcgtc ccgcgcccga gccgctgcaa caggaagagc cggcggccgc
     1021 ctcgcaggag accggagacg tcctggacat accagccttc ctcaggcgca ggtaggcctg
     1081 cgccgaggcg tgcgcagggc cgatacccag accgcattcg gtgccccctc cacgatgcac
     1141 atcgcgagag ggggcgccgt ctacgtcgca ccggacacgg cgcccaaagg agccgggatc
     1201 ttcttcttcg cccgtctcgg tggcacctcg gaacccccat acgactctct gaacgtctcc
     1261 aagaaggtcg gtgacgaccc cagggcggtc gatgagaacc tctcggtgat cgggagcgcg
     1321 atggatctga ggcctgcggc gtgggtccgc caggtcgcgg gggacagtgt ggttcgcgtc
     1381 tgtgagccag gcttcgccgg tgaggccgac gcgctggtaa ctgccgagac ggacctctgc
     1441 ctcagtgtgg ccgtggccga ttgcgttccc gtggcgctcg tcggcgagcg agaggtcggg
     1501 atggtgcact cgggctggcg gggcaccctc tcgggccttt cggggaaggc ggcgaacagt
     1561 ctcggagggg acctcgtgca ggcctatata gggccgtcca tccgccgctg ctgctacgag
     1621 gtgtcggcgg agctcgccgc tcagttcgcc gcggaattcg gggacgaggt ggtctcgggc
     1681 cgccacctct ctttgcagga cgccataagg gtagacctcg aaagggccgg ggtgggggtc
     1741 aacgacctgg gactctgcag cggttgccgg ccggatctct tctactccca ccgcaagcag
     1801 ggtcccctca cgggacgcaa tctagcgtcg gtggtgaggg aggcccgatg agcgtgcatg
     1861 ttacggagat cagggaccga ctcgccggcg tgcacgagaa aatacagagg gctctggaga
     1921 ggagtggacg cggcgaggag caggtccgca tcctcgtcgc caccaagtat tacgacccgg
     1981 agcacctctc cgcactaacc caggcgggcg tggatctcat tggagagaac cgcgcagagg
     2041 atcttctcga aaagcaagcg cttttcggcg accgttttga gtggcacttt atcggtcatc
     2101 tccagaggcg caaggcgaag gtggtagtgc caagggtgac cctggtgcac tccgtggatt
     2161 cggtgcgcct gatcgaggag ctggccaaaa gagcacccga gggtggagcc gacatcctca
     2221 tccaggtaaa cgtggccgat gaggagtcca agtacggcgt caccgaggag gaggttgatg
     2281 agctgttgga cgtcgcggca cagaccgggg ggagagtcag ggctagaggg ttcatgacgc
     2341 tggccccgct ggtcgatgac ccagaagacg tacgacacgt tttcgcgaaa cttcgcacga
     2401 tccgtgatag tctacggaaa agttggagtc cgcacttcga tctctcggaa ctctccatgg
     2461 gcatgagtgg cgattatgag gtagcggtcg aagagggagc gaccgtggtc aggatcgggc
     2521 ggatgctaat agaggaggat gggcccgtaa ggaggcatga tgggagttag ggaccgtctg
     2581 gaccggatcg cggcctggtt cgggttcggt gtcgatgacg aggattacta tgaggacgaa
     2641 gttgaagagg agcgccgtta cgccggcgaa gcctacgccg gcggcgcgcg ccgcggcgcc
     2701 gagccaggac cgaccgtgag acgcctgggc agggccgaac gctcttcggc cttcggtact
     2761 tcactgggag acctcttcgg tagcgaggga tcgggaaggg gagagagcta ccagaacccg
     2821 ccctcgcact tacggccggt ccccgaccag cgtacgaccc gcgtaagcgt cctcgagccc
     2881 tccagtttca acgacgccca gaacctggcg gatcgcttca agcgccagca gccggtgatc
     2941 ctgaacctcc agaatgtcga cggcgacctc agccgcagga tggtggattt ctgctcgggc
     3001 ctgacctacg cgctcgatgg caacatccag tccgtcgcca acagggtctt cctacttact
     3061 ccgcgcgacg tcgaggtgag tgcggaggag cgcaagcgca tcgccgagcg ggcgttcttc
     3121 aatcagctct aaggaacttc aggcgacttt gaagaaggtc gggataatcg gggcgggtaa
     3181 gatcggaggc tccctactct acaggctcgc cgagtctggc ggtttcgacc tcggcgcgag
     3241 cgacattcat cccgaacagc tgaggcccta cgaaagtatc ggcgtccgca cgacctcttc
     3301 gaatagggag ctcgccgccg ccagcgacct cgttatcgcc gccgtcaagc cctgggacgt
     3361 ggcgcaggtg atggaggaga tctcacccac tttcagagac aataacaagg ccgtcacgag
     3421 cgtcgccgcc ggcgtctcca tcgaacgcat ctccgagagc ctgccgcaag ggacggcgat
     3481 cctgcgcgtg atgccgaacg tatgcgcttc ggtgggactg ggctctgccg tagtcacctc
     3541 taatgaggca ggccgcgcgg cgctgcccac ggtcatggat atcttccgcc acgtaggcga
     3601 cgtgctggaa ttgccagaac gcctctttga cgcggcgacg gccctccacg gctccggacc
     3661 ggcgtatgtc gcgctcttcg ccgacgccct gatacaggcc ggtgtaaggg agggcattcc
     3721 acgggacgtc gcgcgcaggc tcgtgatcgg taccataggc gggacggcgg tgctgttgaa
     3781 ggagcggagc gcccaccagg tgcgcgacga ggtgatgacg ccgggcggca cgacggcggc
     3841 ggcgttcgtt gcgatggaga aggctgggtt cgtggcggcg gtctacgacg gggtagaggc
     3901 ggcgaccgag agggcgcgga ggctcggctg atgagcttgc tcctcctgca gcagttcgga
     3961 ttttccgtgg cgagccttct tgcgggggtt gttaactata cctactacat actgttcggt
     4021 ttcatcatcg cctcgatact cttctcctgg ttcccaggct acccttcgag cacctttatg
     4081 caggccatct acgacgcggt caaggccgtg accaacccca tcctttggcc cataaggagc
     4141 cgcgtgccga tgctgcagct cggcgggttc ggtatagacc tctcgccgat catcgcgatc
     4201 ctcgggctct ccatcgcaag gtccctgctt ttgctcgtca ttgaaaactt catagcacct
     4261 gttacggggt gataagagat gcccatacga cccatcgatg taaggcgcaa agagttcaag
     4321 agcagctttc gcggctacga cgcaaaccag gtcgacgatt tcctcgatgc cgtcgccgac
     4381 gagttcgagc gcaactatac ggagaaccag aggatgcgtg aggaggtgtc gagccttcgc
     4441 gaccgcctgc agcagttcga ggacctcgaa ggttcgatcc aggccgccct ggtccacgcc
     4501 gagcaagcgt ccaacgacct ccggagggcc gcggcccagg aggccgaggg tataaagcag
     4561 agcgcccaga gggaggcgga cttcaccatc agggaggcgc agagccgctc tcatcagatg
     4621 ctcgccgact cctcagcccg gatagaacgc gtccaggact cctacgatgc gcttcaggag
     4681 gcgaagcgca gcttcgctaa cgaatttcga cacctcctga agacctacac ggatatcatg
     4741 gagaacatgg aggtggcctc ggccagggag atcgaagcat ccctgcgcgg gcgcctcgac
     4801 accgagtcaa tcgccgtcgt ccgcgaagcc gcccaggagg aagcgcggtc cgcggaagag
     4861 gcgatggtca cggcagaggc gatggccgcc gcgggtatgg taccggagcc ggtgtcagag
     4921 gatgcgcaag acacgctctc ggggctcgag gccgtcgacc ccgcggacca ggaactagag
     4981 ccggcgatgg atgagcagcc aacagaggtg attcggccag aagggagtga acctgagacc
     5041 gaagaccctg aggtcgcgga gcccgaggtg gagccatcca cggcgcagga cgagatggac
     5101 cagcccgttc aagaacctcc tgtctccgtg cccgaggcgg ctagcgaagt agagcgggag
     5161 gagccgtcgc tggctgagga gagcagctcg caacagttct tcgataggca agaaggcgag
     5221 cggtcagaca gtcgcatcta cagggccagc cactttctgc gccggcgtga gtaagggctt
     5281 cgtcgtccac actagtgagg gagccaactt cagagtagac cctgcggagg ccggggagcg
     5341 tgtcgacatg ctggtcgcca ggcggctcgg gatcagccgt agcctcgttc agcgcctgat
     5401 cgcggagaac gccttgctcg tcgatggcat ggatacctct ccttccttca aagtgcggtg
     5461 gggacaacgg gtggaagcgc gcgtccccgt ggagggcata tcgtcagagg agatccccgt
     5521 ccccgtagtc ttcgaggacg agcacatcct ggtcgtggac aaaccagcag ggctcgtggt
     5581 gcacccgggc gctggcaacc tgtcaggcac gctcgtgaac gccctgctga acaggggcat
     5641 tgaaggtggg gatgacccgt tgcgccccgg cgtcgttcac cggctcgacc gcgatacatc
     5701 aggcctgatg atcctggcta aaggcgagcc ggcgtactcc acgctcatcg ggatgatgtc
     5761 ggccaggcga gtaggacgtc actacaggac gatcgtggaa ggggaggggc tgccccggac
     5821 tgggaccata gactcccctg tcggccgtga cccagacaac cccaccctca tggtcgcggg
     5881 cgtagggaag ccggccgtca cgcacttcga ggcgctcgct ggcgcggcag gatacacgat
     5941 gctgcgcgta aggctagaga cagggaggac gcaccagatc agggtccacc tctcggccat
     6001 cgggcatccc gtttacgggg acccgctgta cggcaaaccc gtccccggcc atcgtctctg
     6061 gctgcacgcc gaacggctct acttcgaaca tcctgtaggt ggcgaggcgc tcgagtttca
     6121 ctctgagatc ccccaggact tgcgggactc ggccatcggg cttgacggct ccctcggcct
     6181 ctggtagctt gaccttcggc ataccgatcc tttaacggcg tcccgtgaga cgcgaaggag
     6241 gcaggcttga gccgaacgag cgtctacgag cacgcgcatt ccagggtgct cgcccctgac
     6301 ggactgaacc gttccctacg ccgcatctcg cacgagatcc tggagcgcaa cgccgcgtgc
     6361 ctcgacgggc tcgcgctcgt gggggtgctt acccgcgggg tcccgttggc gaggaggatc
     6421 tcggcgaacg tccgccagtt cgaaggcctt gagctccccg tcggctctct ggatataacg
     6481 ctccatcgtg acgacctcga aaaagtggat cccgaagtga ggggcagcag cgtgccgttc
     6541 gacgtgacgg gcaagaccgt gatcctcgtc gacgacgttc tctacacggg taggaccgct
     6601 cgcgccgcga tggacgcgct cctcgagctc gggcggcccg ccgccatcag gttggcgatt
     6661 ctcgtcgacc gcggccacag agaattgccg atcagggccg actacgtcgg taagaacgtg
     6721 cctaccgcgc gtgacgaaag agtgcttgtc aacctggagg agaccgatgg ggaggacggg
     6781 gtgatcgtcg ttggaaacta ggagctttct aaccctggaa gacatcggcc acgaggacat
     6841 cagggagatc ctcgatctgg caagggcgtt tgcggaggga cgtgtcaggg atgcgctcga
     6901 gggcaagacc gtttgcctgg ccttcttcga ggcgtcgacg aggaccgctg tctcattcga
     6961 gctcgccgcg aggcgttctg gggcgcatgt gatctcactc tccgccaagg gctctgctat
     7021 cgccaagggc gagtccctta tcgacaccgt ggtcaccctc gatcgcctcg gggccgacgc
     7081 gatcgtgctg cggcaccagg ccgcgggagc agcggccctc gcctcccgcc acacctccgc
     7141 gcccgtgatc aacgctggcg acgggtgcgg ccagcacccg acccaggccc tccttgatct
     7201 gtacgccctc tgcgaggcgc tgggcggctt cgaaggactc aggggacgcc gcgccgccat
     7261 cgtcggcgac gtactgcaca gcagggtggc gcgcagcgta atacccggct ttttgcaggc
     7321 cgggatggag gtcgccctcg tggcccccag gacgctactg ccgccggacg caggtgccct
     7381 tgacctaccc gtgctctcct ctgtggacga ggcgctcgag tgggacgcgg atgttctcta
     7441 tatgctgcga ctccagaagg agaggatgac tggcgcgcgg ataccttccg tcgccgagta
     7501 ctccaggttc tacggtgtgt cacggcgcca ccttcagagc ggcgtgctgg tcatgcaccc
     7561 tggcccggtc aacaggggag tggagatctc gggcgacgtc gtcctcgacg cctcgtcgct
     7621 gatccccgac caggtctccg ccggtgtctg tgtccgttcg gccgtcctcg ccctctcgac
     7681 tggcgccgcc cagagcgtcg ccgcgtgatg ctcggggaaa gcaactccgc ggaactggtc
     7741 atccgtcacg cccacatctt cgacccctct acgggcctcg acgggatcac ggacgtacgc
     7801 ctctcatcgg gccgtatcgc ggaggtcggc gagagattac gcggacaacg cgagctggat
     7861 gcagatggcc tgcacctgtt ccccggcttc gtcgacgtcc acgcccactg gcgtacgccg
     7921 gggcgcgaag acgaggaaga tatccagagc ggctcggcag cagcagcagc tggcggcttc
     7981 acgggcgtcg tcatgatgcc gaacacggat cccatcgttg accgccccgt cgtggtcagc
     8041 ggtctggtca gccgcgtgga gcgggagtcg agggtgcggg cgcgcgtctc ggccgcactc
     8101 cacgtggggc tcgctggcga gcggctgacg gagatgcgcc tcctcaagca ggcgggagca
     8161 ttctgcgtct ccgacgacgg gctgggaacg gcttcggctg gcgtcctcag gaacggcatg
     8221 ctctacgccc gttcggcgcg gctgcccgtg atcctgcact gcgaggatca cacgctagcg
     8281 acgggcgtcg tgcacgacgg tgtggccgcc tcactcgccg gacttccagg ttctccggcc
     8341 agcgcggagg acgcagccac ggcgacggcc ctcattctcg ccgccgagac gggagccagg
     8401 gtgcacataa cgcacgtctc cacagccctc tcggccgccc tcgtgggctt cttccggggc
     8461 aaggccgatg tcaccgccga cacgacaccc caccacctga ctttgacaga agacctcgtc
     8521 tcgacactcg acggactcta ccgcgtcaac ccgccgctca ggcccgagct tgaccgcgct
     8581 ggcgtcgagc tggccgttcg cgacggcgtc ctcgacttcg tcgcgacaga ccacgcgccc
     8641 cacgcctcgg aagagaaaga gttgccaata gaagaggccg ctcccgggtt cctcggccac
     8701 gagacggcct tcgccgcgct atacacagat ctggtcctcg gcggcaggct ctcgctgaaa
     8761 cgcctcgtcg aagtgatgag ctgcgccccg ggccgctggg tcggcgcagg aggcacccta
     8821 gccgtcggcg cgccggcaga tctcacgcta atcgaccttt cggaggagtg gacggtaggg
     8881 aggggtacgc tcctcagccg ttcgtccaac tccccctatc tcggacggac gctgaagggc
     8941 agagtggtga gtacaatagt tggtggagag ccggtacacg aaagaacagg agcgaagttt
     9001 ggtgctcgtg cgtaatggag tgcagaccca ggatcggtgc agggcgaggc tggtcctcga
     9061 ggatggggcg gccttccagg gatggagttt cgccggcaac ggggaggtcg ccggcgaggt
     9121 ggtcttcacg acgagcatgg tcggctacca ggagaccatc acggaccctt cttacagggg
     9181 tcagatcctg ctcttcacct acccgctcat cggcaactac ggtgtgatcc ccggcgacga
     9241 ggagtcacgc aaggtgcagg cagcagccgt cctcgcccgc gagtacacgc cacatccgag
     9301 caactgggcg agcgagcgtt ccctggcagc cctgctcgac gagtgcggtg tcctcggcgt
     9361 ggaaggtata gatactcgtg ccctgacgcg gcacctcagg gacagaggtg cgatgcgcgg
     9421 tgtgatctcc acctcggagt cagaccgtaa cgtcctgcgc gagaaagcca aagcccaccc
     9481 ttcgatggtc ggcctggacc tcgcctcgac gagctcggaa ctcacggaac ccacgcagct
     9541 tccagcctta ggagaggaac ggtgccgcgt cgtcgccatc gactacgggg taaagggttc
     9601 tatctaccgc gagctccgta gccgcggagc ctctgtcctc gcgatgcctg gctccaccgg
     9661 ctccgaggag atcctcgccg ccgaacctga tggcctcttc ctctccaacg gccctggcga
     9721 cccggccgcc ctgggagccg cggtagagac cctgcgtccg ctgctcggtg aggtcccgac
     9781 gttcgggata tgcctcggcc accagctcct cgggctcgcc ctcggttgcg agacctacaa
     9841 gttgcccttc gggcaccacg gcgcgaacca tcccgtcagg aacctgaaga cgggacgcat
     9901 cgagataacg agccagaacc acggcttcgc cgttagcgag acttcactct cggatggcgt
     9961 cggactgacc caccgcaacc tctacgacgg caccgtcgag ggcatcgcgt gcccagggaa
    10021 gagagcctgg agcgtgcagt accaccccga atcgagcccg ggcccgaggg actcgggcta
    10081 tctgttcgaa gagttcgtga acggtatctc agagaaggcg ttggcctagc gtgccgcggc
    10141 gcgacgacat ctcgagtgtc ctgatcatcg gctctggtcc gatcgtgatc gggcaggccg
    10201 cagagttcga ctactcaggt acccaggctt gtcacgcctt gcgggaggag ggttaccgcg
    10261 taatcctcgt aaactccaac ccggcgacta ttatgacgga cccggaggtc gcagacacaa
    10321 cctacgtcga gccactgacg gccgaaaccg taaccgagat actgcgccgc gaacgacccg
    10381 acgcgctgct ccccaccctg ggaggccaga ccgccctcaa tctcgccgtc gaactcaacg
    10441 aggcgggcgt cctcgacgag acaggcgtcg aacttctggg agcatccatc ccgtcgatcc
    10501 gtaaagccga ggaccgcaag ctcttctctc acgccatgca ccgaatcggc ctgaatgtac
    10561 ccccgagccg cacggtccga agcctggccg aagccgagca gctggcagcc cagatcggct
    10621 tccccctcat catccgcccc agcttcaccc tcggcggcaa gggaggggcc acggccaccg
    10681 accaccgcga gctccgccgc gccgtcatcg acggcctcga cgctagcccc gttgggagcg
    10741 ccctcgtcga aaagagcgtc gcaggctgga aggagttcga gctcgaggtc atgcgcgacc
    10801 tcgcagataa cgtcgtcgtg atctgctcca ttgagaacgt agaccccatg ggcgtccata
    10861 caggcgactc aataaccgta gcccccgccc aaaccctctc agaccgccag tatcagaccc
    10921 tccgttccgc atctttacgc ataatacgcg agatcggggt ttccactgga ggatccaaca
    10981 tccagtttgc ggtcgaccct gagagtgacg agtactatgt gatcgagatg aacccgcggg
    11041 taagccgctc gagcgcgttg gcgagcaagg cgacggggtt tccgatagcg aagatagcgg
    11101 ccaagctagc ggtggggtac tcgctcgatg agatcccgaa cgacattaca cgtgcgacgc
    11161 ctgcctcgtt cgagcctgcg ctggactacg tggttaccaa ggtaccccgc ttcacattcg
    11221 agaagttccc gggcgccgtg cccaggctca cgaccaggat gcactcggtg ggcgaggtca
    11281 tggcgatagg gaggacgttc acggagagtt tgatgaaagc gatggcatcg ctggaggtgg
    11341 acgcccagga cgtccatgcc ggcctcgacg aaccgagccc gtaccgcgtc ttcgccatct
    11401 tcgatgccct gcgtgccggg atggacatat ctgagatctt cgagcgtacc tccatagatc
    11461 ctttctttat cgcttccata gccaggatcg tggccgcgga aggctccatc tccgaaccat
    11521 tcggcgtcga tgacctgcgc gagttgaaga ggatagggct gaccgacgag actatcgcca
    11581 cggcctcggg gacttcggtt gaggttgtta ggggtgtgag gcgggccctc ggtgtctcgc
    11641 cgacctacaa atccgtcgat acctgcgccg gtgagttccc tgcgctcacg ccctactact
    11701 actcgacgta cgaggaggaa gacgaggtca ggcgcgagga gaacgcctcc gtcgtcgtgc
    11761 tcggcagcgg ccccaaccgc atcggccagg gcatagagtt cgactatgct tgcgtccacg
    11821 ccagttacgc cctggaggag agcggctacg acgcggtcat ggtcaactcc aaccctgaga
    11881 ccgtctccac cgactacgac acctctacac gtctctattt cgagccgctc accgcagaac
    11941 acgtgctaga ggtcataaaa cgcgaggagc cggaaggggt gatcctgcag ttcggcggcc
    12001 agagtcccct caagctcgcg catgagctgg aagaggcagg agtgcgaatc ctcggcacct
    12061 cgccggacgc catagacctg gcggaggacc gctccaggtt cggacggctc ctggcagagc
    12121 tcggcatccc gcatcccagg ttcgggaccg catcgacgcc cgaggaggcg agggaggtgg
    12181 cgcgcgagct cgggtatccg gtcgtcgtgc ggccttctta cgtcctcggc gggcgcagga
    12241 tggagatcgt ctacaacgac gatgacctcg atctctacct caggaccagc gcgggcacag
    12301 gcccaggaca ccccaccctc atcgacaagt ttatggagga ctacacggag gtcgacgtgg
    12361 atgcggtctc ggacggggag gaagtctacg tcggcggcat catggagcac gtcgaggagg
    12421 ccggtgtaca ttcaggcgac tcctcctgcg tgacgccacc gatcacgatc caccgggcgt
    12481 tgttgcggag gatcgaagac tacaccaggc ggttggccct ctccgttgga gtagtcgggc
    12541 tcatgaacgt gcaattcgtg gtaaggggtg acgatgtgct cgtgatcgag tgcaaccccc
    12601 gcgcctcgcg taccgtgcct ttcatctcca aagccacggg agtgccgctg gccaagatag
    12661 cgacccgggt cctcctcggc gagaagctcg gggatatggc cctacgggct tccaccaacg
    12721 ggcacttctc cgtcaaggcg cctgtcttcc cgttcgacag gttcgcggac gtcgacccgc
    12781 tgctcgggcc cgagatgcgc tcaacaggtg aggcgatggg gatagaccgc accttcggcg
    12841 gcgccttcgc gaaggcgctg acggcggcgg ggcaggaact tcccgtctcg gggagggtct
    12901 acatctcagt cgccaacagg gagaagcggg ccgtggtgct catagccagg gccttcgccg
    12961 atctgggttt cgagatctcg gcgagcgagg ggacggctga ggtgctcaag aacaacgggc
    13021 tgccggtcat gatcgtgccc aagatagggg agctcggaga ggacgtgctc ggccttatag
    13081 aggcgggcgg ggtagatctg atcgtaaaca ccccctgggg tcgcgggcct cgcactgacg
    13141 ggtacctgat ccgacgccga gccctcatgc aaggggtccc ctgtattacc acactcgcag
    13201 gggcagcggc cgccgtacag ggcatagagg ccagggtccg aggcggcacc agcagggtga
    13261 attcgctcca gggcctctac gcgacgagag catgaagttc tacagcgttc gggtcgagga
    13321 gcgtgaggag ctgggcagct acaccctgct ccgctacccc tggaccgatg gacccgtcga
    13381 accggggcag ttcgtgatgg cgagggccgc cggttacccc gcgaccctcg accccttcct
    13441 cgcccgcccc ttctccttct acgattacga cgggagaatt gcgagcctcc tcttcgaggt
    13501 gcgtggccgc ggtactgcgc tcctggatct cgcatcaacc atagaggtga caggacccct
    13561 cggaaaaggc ttccgcatcg atcccgggct ggggtctgcc gcccttctcg gaggcggcat
    13621 aggtgtcgtg ccgctcaaga tcctgggccg gcgtctcgac gccgtaggaa ttcctcacga
    13681 catctacctc gggttcgccg acgcaaagcc ggctgctgtc gccagggatt tccctggcgc
    13741 agaggtggca acgatggacg gctcagaggg gacccacggg accgtgctcg acgcgacaga
    13801 ctccctcgaa gggtacgggg ctgtctacgc ctgtgggcca aacccaatgc tggctgctgt
    13861 caagcgcgca gcagggccaa acagtctctg ccagctctct gttgaggagc ggatggggtg
    13921 cgggaacggc tcgtgcaacg ggtgcgtggt cccagtgcgc ggaggcggtt ttatcagatc
    13981 ctgtatcgag ggacccgtct tccattcgga tgttctggcg tggtagtgcg cgagcaaaag
    14041 gccggtgtcg acctcgcggt cgagctctgc gggctgccgc tgcgtacccc cctgatccca
    14101 gcttccggaa cactctccaa agaagccctg ggcgaggtcg aagacgtgta cggggcgatg
    14161 ctacccaaga cgacgacccc ggctgcaagg gccggcaatc caccgccccg catcgccgag
    14221 actgcggcag ggatggtcaa ctctatcggc ctccagaacc caggtgtaca cggcttcttg
    14281 cagggtctgg acgcgttcga ccttggagtc cctattctcg tctccgtggc cggcgatact
    14341 gtcgacgagt tcacccttct ctgcgagatc ctcgccggcg acgaccgcgt ctccgccatc
    14401 gaactgaacc tctcgtgccc gaatgtggag tgtgggggcc tggccttctg cgcagggccg
    14461 gcttccgtcg agcaggtggt cgccgcctgc cgcaccgccg tgcccggtaa acccattctt
    14521 gccaagctca cgaacgaagg tgtcgtggag aacgcccgcg ccgccgagtc ggcaggggcg
    14581 gaaggcttga cggtcataaa caccctccct gcgctcacca tagacgccca cgctcgcaag
    14641 gttctggtgc ggggcggcct ctcaggcccc gcgataaagc ccatcgcgct ccgggccgta
    14701 tacgacgtat cagccgtggt gaacgtaccc gtgataggta gcgggggcgt ggtgagcgga
    14761 acggatgtgg ccgaattcat gctagcgggc gcgacggcgg tgcaggtagg cacagcgagc
    14821 ttcgtcaggg acccgcacga gatactcgac gagtttacgg cctacctgaa agaaagcggt
    14881 cttggcgcgg cagagttgaa gggagcgctg cgtagcgttc gttccgggat gacgggcggt
    14941 caaatctccg gttgacggcg gcttgatgag cccattgaga cgaccacaag catcagagca
    15001 agcacagcga aacctgtggc agaccagagt ccccatcgga ggccgctcag gagggcgtcg
    15061 gcgcctacgg gcccggtcgc agtcgcggca gcgatcacag caccaaccgc cgcaaacccg
    15121 agggcgttgc cgagctccgt ggccgtgctc aagacccctg aagctagacc ccgctcgtta
    15181 tccccaacgc catccgtagc ggcgactgtc aggggtacac tcgcgcttac gaagccggcc
    15241 tctgccaccg ccatcccgag gagtatggcc accagggcat ctttttgggg cattagccac
    15301 gctaccagca ccaacccggc ccccgacatg gcaaatcccc acaccgctgt ccgtctggcc
    15361 ccaagccggg tcgtgaggcg tccggcgacg ggtgaggcga cgacggacgc cgcggtcatg
    15421 gggatgaaga ggagcccggc ctccagcggc gtgcggtcca atgcgtcctg gaagtacagc
    15481 gttaacacat agagttgggc ggcgctgacc atagacttga ggacgactgc caggttcgga
    15541 acggccacgg cgcgccgata caaaatcggg agcgccacca gcggtgtcga agcccggcgc
    15601 tcgtgggcca cgaaaacgcc aaggagtacc gtccccatcc cggccgtgcc gagcgtggcg
    15661 ggagaggccc atccattctt cggggcctcg gagatggcgt agatcagcga ggctagtccg
    15721 agggtggctg tcgctgcccc tgccaggtcc agcgtacggg gggcggcatc gtctttgctc
    15781 tcatcgatcg cgaccggcgc gagagagagc accgcaagcg ccacgggcac gttcactaac
    15841 aacacccacc gccagcccag gaactgggtg atcactcccc ccagcaccat cccgaagacg
    15901 aacccaaccc cagccatcgc gccgtacacg cccatggcgc gattccgctc ctcgccctcg
    15961 gcaaaggtgg ccgccaccag cgcgagcgag gccggtacga gcgccgctcc accaaccccc
    16021 tgcaatagac gcgcagccac cagcacccag ggcgcccacg ccagtccccc aagcagagag
    16081 gcggcggcga acaaggctag cccggacacg aacaggcgcc gccggccgta aaggtcgccg
    16141 gcgcggccca taagcagcag gaggccccca aaggagagcg cgtatccggt aaggatccac
    16201 tgcgcgtcgc cccctgagat accaagctct ctccgtatcg atggcagcgc aatcgcgacg
    16261 atcgtgaggt caaggactgc cacgaacgag gcgcaacaca acagcaccag cgcgaacgtc
    16321 ttcctctccc caccttccga caccataccc tcctccaacg ccctcgtgac atagccgaca
    16381 ctatcaatac gttataccgg tgtcgtagtc tctcaaaacc ttctatcgct tacaatggtg
    16441 tcggactact gaggaggcgc aaggtggagc agcgcatacc ggaaggcgag gttcctgaag
    16501 aggtgaggct cgtcaacgag ttcctcaaca cgctagacct tgagacgtac ggggagcacg
    16561 ctgggaagcc ggacgaggag agggaggggt ttaggagccc cgagaggctc agggcctggt
    16621 tcgtcgggcg tgggcttctc gggagggagg tagcggtgaa tgaggcggat cgtcaactgg
    16681 ctgtacgggt gagagacgcc ctgcgttcag cggctctggc caactcggtt ccggctcgag
    16741 agcggtcgga cgctgccgga tggagcttcc aagagttgcc gctcgtagtc cgcttcgggg
    16801 agaattcgaa acccgagcta gtgagcgggg agggaggggt gagaggggcc ctaggcagga
    16861 tcctcgccga cgttgtggta gcgtcctcga aggggacgtg ggcgcggatg aagatctgcg
    16921 ccgcccagga ctgtaggtgg gcgtactacg accactccaa gagccgcacc ggcaggtggt
    16981 gcgctatgga gacgtgcggc aaccgccaca agaccaggag gtatcgccga aagaggcgta
    17041 gcagcgcctg gcaagaaggt tagaccgagt gccttcctgc gatgatatac taacccgttg
    17101 atgttgaggc gggcaccaga acggacggct gacgaacgca gggacgcgct cgaggaggcc
    17161 aacagggtac gctttgccag ggccgcgacc aagcgcgacc tcaagag
//
LOCUS       DASTKM010000035         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_181649, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000035 DASTKM010000000
VERSION     DASTKM010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181649"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFQ10_01375"
     CDS             complement(<1..312)
                     /locus_tag="VFQ10_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9964019.1"
                     /translation="MITRFDHAVIAVADLERAIEAYRALGFDVSPGGRHEHRGTHNAL
                     IRFGGVDYVELLGVYDPERAVESGPNGRTLAEFVRDRQGGLVGHCYATDDIEAEADRT
                     RE"
     gene            complement(319..1323)
                     /locus_tag="VFQ10_01380"
     CDS             complement(319..1323)
                     /locus_tag="VFQ10_01380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9964020.1"
                     /translation="MGEGNVKFGLTLPNRGVITGATTMDEMLALAQMADKEPIWDSVW
                     VGDSILAKPRLDAIALLGALAVKTERVKLGPACFASTPLRNALLLAYQWASLDYMSGG
                     RTIFVACQGQAAAGRGGNFANEFAAFGIDPSSRMRRMEEAVEIMRLLTSEEEASYEGE
                     YNRFENVTVLPRPVQQPVPIWLVANPDPAKKGNVETSLRRVVRLADGWQTTWNTPESF
                     AQHLSTIKGYAEEEGRDLGENFEACLYYNINVGEAREAALDEAKRFLDAYYYTDYDRE
                     RLKRWVAHGSAEECVEQIQAFIEAGATTVTLRMAAYDQKAQFERVSREVLPALQAALA
                     "
     gene            complement(1361..2644)
                     /locus_tag="VFQ10_01385"
     CDS             complement(1361..2644)
                     /locus_tag="VFQ10_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET9964021.1"
                     /translation="MSSEEPATTFARRDTPQAVGERLRIIGPGLVLAATTVGIGDFIS
                     NAVAGERFGTTFMWAIVLAVVLKYFLTEGLGRWHLASGQTIIYGWNSLGRGVTAFVAV
                     YLVIWAFFFGAAGPSVVGLATNAMFPILPVPAWAVIHSLLAFLFVWVGRYQLFENVMK
                     VLIACKVVVVVVIAALLRPDLGDIASGLVPKIPAGSLLYAVGIVGGLGGTLALASYGY
                     WVRDKGWRSPTWVPIMRLDAGLGYVVTAIFGVAVMVIGAELLFGTGRSIGDEEGLVNL
                     ASPLGERFGTVVRWIFLAGLWAISFASVIGVWNGMSYLFADIVRTFRGIPEEEAGPHI
                     SEKSPAYRSFLILLTFPTIPLIVLGQPVALVLLWTAMGAVFLPFLSITLMWLLNSSRV
                     ERGYRNRPVSASNIVLGVSVVIFLVLGVQTIVGLL"
BASE COUNT          552 a          939 c          847 g          496 t
ORIGIN      
        1 ctccctcgtg cggtcggcct ccgcctctat atcgtcggtg gcgtagcagt ggcccactaa
       61 gccgccttgc ctgtcacgaa cgaactcggc aagcgtacgc ccgttgggac cgctctcgac
      121 tgccctttcg ggatcgtaga cccctagcag ctcgacgtag tcgacgccgc cgaagcgaat
      181 tagggcgttg tgggtgccgc ggtgctcgtg gcggccaccg ggagagacgt cgaagcccag
      241 agcgcggtag gcttcgatgg ctcgctccag gtcggcgacg gcgatcacgg cgtggtcgaa
      301 gcgggttatc atgctactct aggcgagcgc ggcctggagg gcgggcagca cctcgcggct
      361 cacccgctcg aactgggctt tctggtcgta ggcggccatg cgaagggtga ccgtggtagc
      421 tccagcctcg ataaaggcct ggatctgctc gacgcactcc tcggccgagc cgtgagcgac
      481 ccacctcttg agacgctccc ggtcatagtc ggtgtagtag taggcgtcga gaaacctctt
      541 ggcctcatcg agcgcggcct cccgggcctc tcccacgttt atgttgtagt agaggcaggc
      601 ctcgaaattc tctccgaggt cgcgaccctc ctcctcagcg tagcccttga tggttgagag
      661 gtgctgggcg aaggattccg gggtgttcca ggtggtctgc cagccgtcag cgaggcggac
      721 gacacgcctc agcgatgtct cgacgttccc cttcttggcc gggtccgggt tcgctacgag
      781 ccagatgggg accggctgct gcaccggcct cggcagcact gtcacgttct cgaacctgtt
      841 gtactcgccc tcgtagctgg cctcttcctc ggaagtgagt agccgcatga tctcaacggc
      901 ctcctccata cgcctcatcc tcgaggaggg gtcgatcccg aaggcggcga actcgtttgc
      961 gaagttgccg ccgcgtccgg cggccgcctg tccctggcag gcgacgaaga tcgttctccc
     1021 gccggacatg taatcgaggc tggcccactg gtaggcgagc agaagggcgt tcctcaaggg
     1081 agtactcgcg aagcaggctg gacccagctt caccctctcg gtcttgaccg ccagagcccc
     1141 gagcagcgct atggcgtcga gccttggctt ggcgaggatc gagtcgccca cccacaccga
     1201 gtcccagatc ggctccttgt cagccatctg agccagtgcc agcatctcat ccatggtggt
     1261 agcacccgtt atgacccccc ggttcgggag tgtcagccca aacttgacgt tgccttcgcc
     1321 caccgtctct acctcctcga gtgttcgtga gcaactaagt tcacagaagc cctacgatag
     1381 tctgtacccc caataccaaa aagatgacga cgcttacccc caggacgatg ttagaggcgg
     1441 agaccggcct gttccggtaa ccgcgttcca cgcgggagga gttgagcagc cacatcaagg
     1501 tgatagagag gaagggcaaa aacacagccc ccatcgccgt ccagaggagc accagggcta
     1561 ccggctggcc gagcacgatt agaggtatcg tcgggaaggt gagaagtatt aggaaggagc
     1621 gataggccgg gctcttttct gagatgtggg gtccggcctc ttcttccggg atgccccgga
     1681 acgtcctcac tatatcggcg aagaggtagg acatgccgtt ccacaccccg atcaccgagg
     1741 cgaaagagat cgcccacaaa ccggcgagga agatccagcg cacgacggtc ccgaaccgct
     1801 cgcccagcgg gctggccagg ttgaccaagc cctcctcatc acctatgctc ctcccggtgc
     1861 cgaagagcag ctcggccccg atcaccatca ctgccacccc gaagatggcc gtgactacgt
     1921 atccgaggcc cgcatctagg cgcatgatcg gtacccaggt cgggcttctc cagcccttgt
     1981 cgcgcaccca gtagccgtaa gacgccaagg ccagcgtgcc gcccaatcct ccgacgatgc
     2041 ccacggcgta cagaagtgaa ccggccggga tcttcggcac cagacccgag gctatgtccc
     2101 caaggtccgg ccgcaacagc gcggcgatga cgaccacgac gacgacctta caggcgatca
     2161 gcaccttcat gacgttctcg aacagctggt atctacccac ccagacaaac aagaacgcga
     2221 gcagggagtg gatcaccgcc caggctggga ccggcaaaat ggggaacatg gcgttggtag
     2281 ctaatcccac gaccgacggg ccggcggcgc cgaagaagaa cgcccagata acaaggtaga
     2341 cggccacgaa agcagtcacc ccccgtccca gagagttcca cccgtagatg atcgtctggc
     2401 ccgaagccag gtgccaccgc cccaacccct ccgtcaagaa atatttgagc accacggcga
     2461 ggacgatcgc ccacatgaag gtcgtcccga agcgctcccc agccaccgcg ttggagatga
     2521 agtcgccgat gcccaccgtg gtggccgcga gcaccagccc cggcccgatg atcctcagac
     2581 gctcccctac cgcttgcggg gtgtctctcc gagcgaaagt ggtagctggc tcctcgctgc
     2641 tcaagcgttc ccttctctca agaacgacat ctcatgattg gcgacgcctg cacaagaggg
     2701 gagcgtcccc ggcagggttc ccaagacgcc catcaccttg ggccgcactt tcgacggtaa
     2761 atccggctcg gtttgtcgga ttatagccct cgctgcggat cggtcaacgg ttagttatgt
     2821 ccagccaacc cgcg
//
LOCUS       DASTKM010000036         3500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1239208, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000036 DASTKM010000000
VERSION     DASTKM010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239208"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /gene="gnd"
                     /locus_tag="VFQ10_01390"
     CDS             complement(<1..866)
                     /gene="gnd"
                     /locus_tag="VFQ10_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET9964022.1"
                     /translation="MEIGIWGMGRMGFNMARRLVDKGDHRVIAGNRSPGKVDEAVEHG
                     AEGAYSLEEFVVMQDSPRVLWTMLPAGDVTDDIVRQFTDLAEEGDIIVDGANSYFRDS
                     VARAREVRNAGLRWLDAGVSGGVWGYDVGYCTMIGGDADAFEYVEPVFETLAPPNGYA
                     YLGDAGAGHFAKMVHNGVEYGMLQAYAEGFEILQKSRYDYDLRALSSLWNQGSVVRSW
                     LLELAERAFEKDANLDSLSGYVEDSGEGRWTVLEAIEENVPANAIAGSLFARFASRQD
                     DSFAMKVIAALRG"
     gene            complement(922..2016)
                     /gene="tal"
                     /locus_tag="VFQ10_01395"
     CDS             complement(922..2016)
                     /gene="tal"
                     /locus_tag="VFQ10_01395"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET9964023.1"
                     /translation="MNERLARMRELGQAPWVDELSREDIKNGGLRRMIEDGIAGVTSN
                     PAIFQKAIAGSDLYDEQLGELAERVDDPKQIFWEVARRDIQDACDIFMAVHEETGGQD
                     GFVSLEVQPDIAYDTEATVEEATRLHEMADRPNLFVKIPATLPGLVAIEEMISRGKSI
                     NVTLIFSLERYREVARAYTRGLQRLVENGGDPSGVHSVASFFVSRIDTEADKQLEELG
                     AEEYKGKLAIANAKVAYQIYKQVFGGARWRSLEDKGANRQRLLWASTSTKNPDYQDTI
                     YVDNLVGPETVNTMPKKTIQAVMEHGDIRPTLEEGVEEAKRLLERLREVGLDYEDVTD
                     ILEKEGIQKFADPFNELLEEIENKSRQLVS"
     gene            complement(2536..3018)
                     /gene="ssb"
                     /locus_tag="VFQ10_01400"
     CDS             complement(2536..3018)
                     /gene="ssb"
                     /locus_tag="VFQ10_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET9964024.1"
                     /translation="MVSFNRVVLAGNLTRDPELRFTQSGTPVCGFGLAVNRVFSKNDE
                     VDFFNISAWRDLGERVANYTKKGDPVLVEGRLQYRTWEAQDGSKRSAVDVVADNVQFL
                     GRAGDADEGASSGGEGGQARRGGGRGGRREEVDINEEDFEDIPFSHFSVKPLIARNIL
                     "
     gene            complement(3120..>3500)
                     /locus_tag="VFQ10_01405"
     CDS             complement(3120..>3500)
                     /locus_tag="VFQ10_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9964025.1"
                     /translation="GGLFSEINDNSVAILLTYGTARILLAGDAEANEEEYMANGPYTG
                     PLTILKVTHHGSNTSSTPLFLSRFPPQVAVIQCGADNPYGHPTPETLDRLQRAGAKVF
                     RNDEDGDVIATIKDQELKVATTKR"
BASE COUNT          584 a         1142 c         1001 g          773 t
ORIGIN      
        1 ccgcgcaggg cggctatgac cttcatggcg aaggagtcgt cctggcggga ggcgaagcgg
       61 gcgaagagag agcctgctat ggcgttcgcc ggtacgttct cttctatggc ttcgaggacc
      121 gtccagcggc cctctcccga gtcctcgacg tagccgctaa ggctatcgag gttggcgtcc
      181 ttctcgaagg cgcgctcggc gagttcgagg agccagctac ggacgacact cccctggttc
      241 cacaggctcg agagggccct gagatcgtag tcgtagcgag acttctggag gatctcgaac
      301 ccttcggcat aggcctggag catcccgtac tcgacgccat tgtggaccat cttggcgaag
      361 tgtcctgcgc ccgcgtcccc gaggtatgcg tatccgttcg gaggcgcgag ggtctcgaag
      421 acaggctcga cgtactcgaa ggcgtctgcg tcgcccccga tcatggtgca gtagccgacg
      481 tcgtagcccc agacgcctcc tgacacgcca gcatccagcc acctcaagcc agcgttcctg
      541 acctcccttg ccctcgccac agagtccctg aagtaagagt tggccccgtc cacgatgatg
      601 tcgccctcct cggcgagatc tgtgaactgc ctgactatat cgtccgtcac gtccccggca
      661 ggtagcatgg tccaaagcac cctcggcgag tcctgcatca ctacgaactc ctccaggctg
      721 taggcaccct cggccccatg ctcgactgcc tcgtccacct tgccgggcga acggttgccc
      781 gcgatcaccc ggtggtcgcc cttgtcaacg agccgccgcg ccatgttgaa ccccatccgc
      841 cccatgcccc agattcctat ttccatgtgt tgcctccctc cgctcagcgc ttgtcagctt
      901 gtcagcacgc tgacaggctg actagctgac aagctggcgg ctcttgttct cgatctcctc
      961 tagcaactcg ttgaaggggt cggcgaactt ctggatgcct tccttctcca ggatgtcggt
     1021 tacgtcctcg tagtcgaggc cgacctccct gagccgctcg aggagtctct tggcctcttc
     1081 gacgccctcc tcgagggtag gcctgatgtc gccgtgttcc atcaccgcct gtatggtctt
     1141 cttgggcatg gtgttgacgg tctctggccc gacgaggttg tccacataga tagtgtcctg
     1201 gtagtcaggg ttcttggtcg aggtcgaggc ccagagcagg cgctggcggt ttgcgccctt
     1261 atcctctagc gaacgccacc ttgcgcctcc gaagacctgc ttgtagatct ggtaggccac
     1321 cttggcgttg gctatggcga gcttgccctt gtactcttcc gccccgagct cttctagctg
     1381 cttgtctgct tcggtgtcta ttctggagac gaagaagctc gccacggagt gcacgcccga
     1441 gggatctccc ccgttctcga ctagtctttg gagacccctt gtataggcgc gggccacttc
     1501 gcggtagcgc tcgagggaga agatcagggt gacgtttatg gacttgccgc gggagatcat
     1561 ctcctctatc gccacgagcc ctggcaaggt ggccggtatc ttgacgaaga ggttgggcct
     1621 gtcggccatc tcgtgcagcc gcgtggcctc ctcgaccgtg gcctccgtgt cgtaggcaat
     1681 gtcaggctgg acctctaagg agacgaaccc gtcctgtccc cctgtctcct cgtggaccgc
     1741 catgaagatg tcgcaggcgt cctggatgtc ccgcctggcg acctcccaga atatctgttt
     1801 cgggtcgtct actctctcgg ccagctcccc cagttgctcg tcgtacaggt cggagccagc
     1861 gatggccttc tggaagatag ccgggttgga ggtaacgccc gcgatcccgt cttctatcat
     1921 acgccggaga ccgccgttct ttatgtcctc acgcgagagc tcgtcgaccc agggcgcctg
     1981 ccccagctca cgcatcctcg ccaaacgctc gttcacctgt cgcctcctta cgaagtcatt
     2041 acggggacgg tagtgcatcc cattcgttca gtctacacct cgatacgtaa ggcatccctc
     2101 ctctggagcc acgtctcgta tcgtgtatgc gctccttacc atcgaaggtc gaagatcaaa
     2161 ccaggcgttg taccttctcc ggcatgacgc ggaacacccg catgccctct cgagatatgg
     2221 cagccctggg cgagttgccg gaaggctcac ctggagtcgg gcgccaggcc caggagtact
     2281 taggctacgc gcagtcttat tcgctgtcgg aattacttgc caccgggcgt cctccaaacc
     2341 tccggcgccg acctggctac gagagcgact ggttctcctc gaaatctgcc cccggagcag
     2401 cggagatctg gcaagcctgc gccagtacgc tacctactcc cggcacgatg cttcgcgaag
     2461 ggtattcgag gatgcttgta gaatgacgag agtgcctttt cggcccctca gtcccttcag
     2521 accttcgacc ccaccctaca agatgtttcg ggctatcaac ggcttgacac taaagtggct
     2581 aaatgggata tcttcgaagt cttcttcatt gatatccact tcttcacgcc ggccgccgcg
     2641 cccgcctccg cggcgggcct gtccgccctc acctccggag gaggcgcctt cgtccgcatc
     2701 tccggctcgg ccgaggaact gcacgttgtc ggcgaccacg tcgacggcgc tccgcttgga
     2761 gccgtcctgg gcctcccagg tacggtactg caggcgacct tcgacaagta cgggatcccc
     2821 cttcttggtg tagttcgcaa cacgctcgcc gagatcccgc cacgcggaga tgttaaagaa
     2881 gtctacctca tcgttcttag agaacacccg attcaccgcc agcccgaatc cgcacaccgg
     2941 cgtgccgctc tgcgtgaacc ttagctcagg gtctctggtc aaattaccgg ccagtacgac
     3001 acgattgaag ctgaccacgt ctctcctcct tctctgtgcc ttacaagggt aacaatacag
     3061 gaacattctc ggttctcaag cgctatcgct ttagccaggc gtgggtggag ttgcgctctt
     3121 caacgtttcg ttgtggccac cttcagctcc tggtccttta tggtggcgat cacatccccg
     3181 tcctcgtcgt tgcggaagac cttcgcgcct gccctctgca gtctgtcgag cgtctctggc
     3241 gtgggatgcc cgtaggggtt gtccgcgccg cactggatga cggccacctg tgggggaaag
     3301 cgactgagaa agagtggggt cgatgaggtg ttggagccgt gatgggttac cttcaggatc
     3361 gtcagtggac ccgtgtaggg tccgttcgcc atgtactcct cctcgttcgc ctcggcgtcg
     3421 ccggccagca ggatgcgggc cgtgccgtag gtcagtaaga tagccacgga gttgtcgttg
     3481 atctcggaga acagaccgcc
//
LOCUS       DASTKM010000037        10752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1063255, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000037 DASTKM010000000
VERSION     DASTKM010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063255"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..776)
                     /locus_tag="VFQ10_01410"
     CDS             complement(237..776)
                     /locus_tag="VFQ10_01410"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HET9964026.1"
                     /translation="MEGPLLTLTLVLMFVGLLGSVLPGLPGVTLIFLSALVYAIITDF
                     RTVGAAILVILFIFAAIAFVADFVATSYGARRFGASNWGTVGGAVGGIAGALIGLLFL
                     GIGSLFGLILGTIAGVFLGEYLKRTRQGRQEPGSAGSDWRRASHAAGGVLVGYLAAAI
                     IQGLLGLASVVVFVLALVY"
     gene            complement(780..1634)
                     /gene="larE"
                     /locus_tag="VFQ10_01415"
     CDS             complement(780..1634)
                     /gene="larE"
                     /locus_tag="VFQ10_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HET9964027.1"
                     /translation="MNPLATDAPQKRLAELEQILAPHGSALVAFSGGVDSSLALAAAA
                     RALPKHRVLAVTSNNETYLPSELDLAREFAESLGVEHLVINTRELDNPDYASNPTNRC
                     YFCKSTLYSDLAKLAEQKGYACVVDGANKDDEGDYRPGRRAAREIGVVSVLSEAGMTK
                     AEVRELAKHLGLPTWDKPALACLSSRFPYGQEITPEKLSQVARAEEFMRRKGFSQVRV
                     RHHGEIARLEVGPEELERAFAMREEISAELLDAGFLYVTLDLAGYKSGSLNAALKKGK
                     QALPLISS"
     gene            complement(1648..2220)
                     /locus_tag="VFQ10_01420"
     CDS             complement(1648..2220)
                     /locus_tag="VFQ10_01420"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9964028.1"
                     /translation="MSSQENVYEVPEGLPEPEDDGACDHLPGMRMPSVSLRSTAGDPV
                     DLSTLTGTTVVFCYPLTGRPDQDLPPGWDDIPGARGCTPQSCAFRDHHAELQELGARV
                     FGLSAQDTAYQREAAERLHLPFLLLSDENLEFARALELPTFEAEGMTLIKRLTLVVEG
                     GEILKVFYPVFPPGRNAEDVLSWLSERSVT"
     gene            complement(2220..3461)
                     /locus_tag="VFQ10_01425"
     CDS             complement(2220..3461)
                     /locus_tag="VFQ10_01425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET9964029.1"
                     /translation="MADRRPEAYITGIGAVTPIGVGRKNLWSSLLANESGAGPITLFD
                     PQDFEVRIAAECTDFDPENFMDRKVAARTDRFGQMGLASAKLALEDAGAWDVLNNNPE
                     RVGLILGSGIGGVTSMETTQATIDSKGPSRVNPFAVTKIMPNSAAAHVAIQLGVQGPS
                     ITGALACTCGTDMMGLGYDLIRRGDADVVLCGASEAALAPVIVAGFIAMRAMSRRNDD
                     PEGACRPYDVDHSGFLIGEASAVVVLESAESVEHRGSEPYAKITGVGRTTDAYNLTDP
                     DPKGFGIMRAMQLAVADAGLRGEQIGYINPHGTGTVAGDGPESQAMAKINPNVKVSAT
                     KSTLGHSLGASGAVETAICALAIKERKIPPMRNLTKVAEGCADLDYVVDEPREAPDLE
                     AALCANLGVGGHNAAVVLERA"
     gene            3572..3892
                     /locus_tag="VFQ10_01430"
     CDS             3572..3892
                     /locus_tag="VFQ10_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964030.1"
                     /translation="MERALIFYIAMVLFVANFALGVLVQFRIVDTNPFRWLHHALFFA
                     VFASAAIAVGVGFLQGAPYRWVLLPVLVLFAFLPRVRAGTSGHAALASGAMILYVTGF
                     AWML"
     gene            3903..4715
                     /locus_tag="VFQ10_01435"
     CDS             3903..4715
                     /locus_tag="VFQ10_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET9964031.1"
                     /translation="MDFLEVLKRRKTTNGPFLPDPVKPEHQRLLVEVAAMAPSHFNSQ
                     PWRFVLVDDRETIDEVARISSDSMTRLLEEGTFWKRYRPYFRFSEAEMDEKRDGIHFD
                     QMPSVLKPFSRQIFSNTGQAVMNRFGVPSTLGEDNRKLVAGSPLLLAVLLDKTEYRPG
                     ELSGFYSIFGMGAAVENIWLTTTELGMGIQFVSTPMEIPENWERLKRLLKVPDDLELM
                     AVYRLGYVPDGQRRPTIDWSSRQRKRISQYVFRDTCEKPEPDFATQTLLDER"
     gene            4712..5758
                     /locus_tag="VFQ10_01440"
     CDS             4712..5758
                     /locus_tag="VFQ10_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET9964032.1"
                     /translation="MNAPRILSVASAVPPHRVGQEEAKTFARSMFSCGHGDIERLLPI
                     FDNVHIENRYFCVPAEWFEQDHTFSEKNALYVEHALDLSEKAARRALDRVGAGPDEVG
                     AVFFVSTTGISTPSIDAKLIFRLGLSEHTRRVPIWGLGCSAGAAGIARAAEHASLYPD
                     QLVLLVAVELCGLTFQQGDRSKSNLISTSLFADGAAAVLLGGTTGPEILGSYSTTWPD
                     TEAVMGWDLVETGLKVQLSKSIPNIVRDKFRDNLEEACNSLGLEFDELEHFVLHPGGS
                     RVLDAFEGALGLQAGGLTTSRDVLRDCGNMSSVTVLFILERFLDEGELAKDDLGVLSA
                     LGPGFSAEHVFFRC"
     gene            5859..6374
                     /locus_tag="VFQ10_01445"
     CDS             5859..6374
                     /locus_tag="VFQ10_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9964033.1"
                     /translation="MNVAVLVAGVALVAVQRLLELTYSRRNERRLRSHGAVERGAGHF
                     PVIVAIHTLWLVSTLVEGLLRGPEPPVWWPVPLAAFLLVQPLRYWAIFSLGVNWNVRV
                     LVVPGGKLVRRGPYRYFPHPNYVVVVVEVLTFPLIFGAWITAIVFSLLNAALLYVRIR
                     TENRALRELSS"
     gene            6599..7867
                     /gene="serS"
                     /locus_tag="VFQ10_01450"
     CDS             6599..7867
                     /gene="serS"
                     /locus_tag="VFQ10_01450"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET9964034.1"
                     /translation="MLDLKYIRENAKAVQENTRNRGVEADVGLVVELADRRSDLIQES
                     NELKQRQNQMARSIGRERDEEARARLIEESRAIKERLPSKERELHEVEERLRDEQSKI
                     PNLTHPDSPIGKDDTENVEIRRWGDVPAFPFEPKDHVELGEQLGIIDFDAGAKTTGTK
                     FYFLRGDAVLLELGLIRYAMDILIERGYQPTITPDLARDRMLVGTGFIPRGPETQIYS
                     VEGMDLSLVATAEIPLAGQHADEIIEERDLPLRYAGLSHCFRTEAGSHGRASRGLYRV
                     HQFTKVEMFAFTTPDQSEDMHDEMVGIEERIFQGLGLPYRVVDICTGDLGGAAYRKYD
                     LEAWMPGRGDFGEVTSTSNTTDYQARRLAIRYRKEGGRPQLLHTLNGTALAISRALIS
                     LLEIYQQQDGSVMLPEVLVPYVGRERLTSS"
     gene            7938..9368
                     /gene="proS"
                     /locus_tag="VFQ10_01455"
     CDS             7938..9368
                     /gene="proS"
                     /locus_tag="VFQ10_01455"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET9964035.1"
                     /translation="MTEAVEKLVKKSEDPSRWYLQLVRMAKLADYGPVRGTFAIRPYG
                     FEIWERVQRDLDDRFKETGHKNAYFPLLIPESYLRKEAEHVEGFDPELAWVTIGGREE
                     LEERLAIRPTSESIICDFYRNWIQSYRDLPILINQWCNVLRWEKVTRPFLRTAEFLWQ
                     EGHTVHATAEEAREETLRMLGVYRDCFYETMAIPVLTGAKSPSERFAGAVETYTCEGL
                     MGDGRALQAATSHDLGQNFARAFDITFLDENQERVHPYQTSWGFSTRTIGGLILVHGD
                     DRGLRLPPRIAPTQAVIVPIWRGKNKGEVRREAETLHAELKDSGIRMEVDLDEEHSPG
                     WKFNEHELRGVPVRVELGPRDIEKDQAVLVRRDTGEKEFVDRKELPDRLEAVMGEIQD
                     NMLRQATEFRDANIRRAESYEEFEEIIARERGFVVAPWDGTEETEQKIKDDTKATIRL
                     LPFEREEGKDLVSGRPGRTALFARAY"
     gene            9583..10470
                     /locus_tag="VFQ10_01460"
     CDS             9583..10470
                     /locus_tag="VFQ10_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964036.1"
                     /translation="MPALILLAGDHVAVEAEVYSSYSDAKKPVIAVILSEQQNVDEFK
                     AHFGLSARQVDAVLTATRHENRVLAQVFGESEIVLAANRDLPKKEIAKKIAASDYDEK
                     VAAAVARTKQRIEAIVPEDQSADLEGWVDEQWNQEVLAASEEDYTASSETYMVSSQRK
                     GIRCRVFATQYTGYTEREVALPHRNLKIGRQPRVRIWRVSGGPSVGPRVKEVGPWNTY
                     DNYWVQNKDRTMYKRVPRCKPAAQVAYYQNFNKGKDEFGREVLNPAGVDLTPDVARSM
                     GLEEYQNAWVWVRFPWVHR"
     regulatory      10554..10678
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2032 a         3292 c         3468 g         1960 t
ORIGIN      
        1 tcccaggcag atcagggtcc gcgcaacctg ccctccggcg acgaactcgc ccaggaacta
       61 gaaggattcc tccgcgaaca ccgtcgcgaa gaagatagcg agtaagcaag tctgcaagtc
      121 ctcgcacacg cgaaagctga cgtgctgaca agcgaaggcg aagcctgagc gtgctgacaa
      181 ggcgcgaagc gccgtgctga aatgctgaca agacggctgc aaggcttgtt gacgtgctaa
      241 tagaccagcg ccaacacgaa gactacgacg cttgctagtc cgagcagccc ctgtatgatg
      301 gcggcggcca ggtagccgac gaggacgccg cctgctgcgt gcgaggcgcg gcgccagtcg
      361 gacccggccg agcctggctc ttgtcggccc tggcgggtgc gcttgagata ttcgccgaga
      421 aagacgcctg cgatggtgcc aaggatgagg ccgaacaggg agccgatgcc gaggaagagg
      481 agcccgatca gggcgccggc gatgccgccg acggccccac ccacggtccc ccagttgcta
      541 gccccgaagc ggcgggcgcc gtatgaggtg gcgacgaagt cggcaacgaa ggctatcgcg
      601 gcgaatatga ataggattac gaggatcgcg gctccgacgg tgcgaaagtc cgtgatgatg
      661 gcgtagacga gggctgacag gaatatgagc gttacgccag gaagaccagg caagacgctt
      721 ccgagcaggc ccacgaacat caagacgagg gtgagggtga gcagcgggcc ctccatactc
      781 taagacgaga tgaggggaag ggcctgcttg cccttcttca gggcggcgtt caggcttcct
      841 gacttgtagc ctgccaggtc gagggtgaca tagagaaagc ctgcgtcaag gagttcggcg
      901 gagatctcct ccctcatggc gaaggccctt tcgagctcct cggggccgac ttcgaggcgg
      961 gctatctccc catggtgcct gacacggacc tgcgaaaagc ccttcctgcg cataaactcc
     1021 tcggcgcgag cgacctggga gagcttctcg ggtgtgatct cctgcccata tgggaagcgg
     1081 ctcgacaggc acgccagagc cggcttgtcc cacgtcggca gaccaaggtg cttggctagc
     1141 tccctgacct cggccttggt catcccggct tccgagagca cggagacgac cccgatctct
     1201 ctggcggctc tcctgcccgg acggtagtca ccctcgtcgt ctttgttcgc cccgtcaacg
     1261 acgcaggcgt aacctttctg ctctgcgagc ttcgccaggt cagagtagag cgtgctcttg
     1321 cagaagtagc agcggttcgt ggggttgctc gcgtagtctg ggttgtcgag ctcacgggta
     1381 tttatgacga ggtgctccac cccgagagat tcggcgaact cccgcgccag atccaactcc
     1441 gatggaaggt aagtctcatt gttggacgtg acggcgagga ccctgtgttt cggcaaagcc
     1501 cgcgctgcgg cggccagggc gagcgaggag tcgactccgc cggagaaggc cacgagcgcg
     1561 ctaccgtggg gggcgaggat ctgttcgagt tccgcgagcc tcttctgcgg agcatcggtc
     1621 gccaagggat tcatctacac ttcctctcta ggtcacgcta cgttcggaga gccaactcag
     1681 gacatcttcg gcgttcctgc ccggtgggaa cacaggatag aagaccttca ggatctcccc
     1741 tccctccaca acgagcgtca ggcgttttat gagggtcatg ccttcggcct cgaacgtcgg
     1801 cagctctagg gctctcgcga actccaggtt ctcatcgctc aacaacagga acggaaggtg
     1861 aagtcgttcg gcggcctcgc gctggtaagc ggtatcctgg gcactcaggc cgaagacccg
     1921 tgccccaagt tcctgcagtt ccgcgtggtg gtcgcggaag gcgcacgact ggggggtaca
     1981 accgcgggcg ccggggatgt cgtcccaccc tggcggcagg tcctggtcag gcctccctgt
     2041 aagcgggtag cagaaaacga cggtcgtccc cgtcagggtc gagaggtcta cggggtctcc
     2101 cgccgtggac ctcagggaca cggaaggcat ccgcatgcct ggcaagtggt cgcatgcgcc
     2161 gtcgtcttcc ggttcgggta ggccctctgg aacctcgtaa acgttctcct ggctgctcat
     2221 caggctcgct ccaggacgac tgctgcgttg tggccgccga cgccgaggtt ggcgcagagg
     2281 gcggcttcga ggtcgggcgc ctcgcgcggc tcgtccacga cgtagtcgag atcggcgcag
     2341 ccttccgcta ctttcgtgag gttcctcatc ggggggatct tcctctcctt tatggcgagg
     2401 gcgcagatcg cggtctccac ggcgccgctc gcgcccaaag agtggcccag ggtggatttc
     2461 gtggccgaga ccttgacgtt ggggttgatc ttggccatcg cctgcgactc ggggccgtca
     2521 ccggccacgg tacccgtgcc gtgcgggttt atatagccga tctgctctcc tctgagtccg
     2581 gcgtcggcga cggcgagctg catggcccgc atgatgccga agcctttcgg atctgggtcc
     2641 gtgaggttgt acgcgtcggt ggtgcggccg acgccggtga tcttcgcgta aggctcggac
     2701 ccgcggtgct ctacggactc ggcgctctcc aggaccacca cagccgatgc ctcgccgatc
     2761 aggaaccccg agtggtcgac gtcgtagggc ctgcacgctc cttcggggtc atcgttacgg
     2821 cggctcatcg cccgcatcgc tatgaatcct gcgacgataa cgggtgccag ggcggcctcc
     2881 gaggcgccgc agagcacgac gtcggcgtca ccgcgccgga tcaggtcgta gcccaggccc
     2941 atcatgtccg tgccgcaggt gcaggccagc gcgccggtga tcgagggtcc ctggacgcct
     3001 agctgtatgg ctacgtgggc cgcggcggag ttgggcatga tcttggtcac ggcgaagggg
     3061 ttgacgcggg agggaccctt actgtctatg gtggcctggg tcgtctccat gctggtaacg
     3121 cccccgatgc cgctgccgag gatcaggcca acccgttccg ggttgttgtt gagcacgtcc
     3181 caggcgccgg cgtcttcgag ggccagcttg gccgaggcga ggcccatctg cccgaaccgg
     3241 tcggtcctgg ccgcaacttt ccggtccata aagttctcgg ggtcgaagtc cgtgcactcg
     3301 gcggcgatac gcacctcgaa gtcctgggga tcgaagagcg ttatggggcc ggccccgctc
     3361 tcgttggcca ggagcgagct ccagaggttc ttgcgcccta ctcctattgg ggtgaccgca
     3421 ccgatccccg ttatgtaagc ttccgggcgc ctatccgcca tcttgccccc tagcaaaatc
     3481 cacgatccat cgaactacga atcttagcat cacaggcccc cttcgactcc ggcgcgaccc
     3541 gccctgtgct ataaccctcc aggatgagtc ggtggagcga gcattgatct tctacatcgc
     3601 gatggtcctc ttcgttgcga actttgcgct cggcgtactc gtgcagtttc gtatcgtgga
     3661 tacgaacccc tttcgctggc tccaccacgc cctgtttttc gcggtcttcg cctccgctgc
     3721 catcgcggtc ggcgtcggat tcctgcaggg agcgccctac cgctgggttc tcttgcctgt
     3781 ccttgttctc ttcgccttcc tgccgcgggt acgggccgga acctccgggc acgccgcgct
     3841 agccagcggg gccatgatcc tctacgttac aggattcgca tggatgcttt aggaggctga
     3901 gattggattt cctggaggtc ctcaagaggc gcaagaccac gaacggcccc tttctgccag
     3961 accctgtgaa gcccgagcac cagcgcctgc tcgtcgaggt cgccgcgatg gcaccttccc
     4021 acttcaacag ccagccctgg cgcttcgtgc tcgtcgatga ccgcgagact atagatgagg
     4081 ttgcgaggat aagcagcgac agcatgacgc gccttctgga ggagggcacg ttctggaagc
     4141 gctacaggcc ctacttccgt ttctctgaag ccgagatgga cgagaagcgc gatggcatcc
     4201 acttcgacca gatgccaagc gtcctcaagc cgttctcgcg ccagatcttc tccaacacag
     4261 gccaggccgt aatgaaccgc ttcggcgtcc ccagtacttt aggcgaggac aacaggaagc
     4321 tcgtcgcggg ctcgccgcta ctcctggcgg tcctcctcga caagacggag tatcgtcccg
     4381 gcgaactctc gggtttctac tccatcttcg gtatgggggc cgccgtcgag aacatctggc
     4441 taaccacgac ggagcttggc atgggcatcc agttcgtctc gacgccgatg gagatacccg
     4501 agaactggga gcggctgaag cggctgctga aagttcccga cgacctggag ttgatggccg
     4561 tctaccggct cgggtacgtc ccggacgggc agcgaaggcc gacgatcgac tggtcgagcc
     4621 gccagcgcaa gcgtatctcg cagtacgtgt tccgcgacac ctgcgagaag cccgagcccg
     4681 acttcgccac gcagacgttg ctggacgaac gttgaacgca cccaggatcc tctccgtcgc
     4741 cagcgccgtg ccgccccacc gggtcgggca ggaggaagcg aagacgttcg ccaggagcat
     4801 gttctcctgc gggcacggtg acatcgagcg gctgctccca atattcgaca acgtccacat
     4861 agagaaccgc tacttctgcg tgcctgctga gtggttcgag caagaccaca ccttctccga
     4921 gaagaacgcc ctctatgtgg agcacgccct cgacctctcc gagaaggccg cgaggcgggc
     4981 gctggacagg gttggggctg ggccagacga ggtcggtgcg gtcttcttcg tctcgacgac
     5041 cgggatctcg acaccttcca tcgacgcgaa gctgatcttc cgcctcggcc tctcagagca
     5101 cacgcggcgc gtcccgatct ggggcctcgg ctgctcggcg ggggcggccg gtatcgcgcg
     5161 cgccgccgaa cacgccagcc tctatccaga ccaactggtc ctcctcgtcg ccgtcgagct
     5221 ctgtggcctg accttccagc agggcgaccg ttcgaagagc aaccttatct ccaccagcct
     5281 cttcgccgac ggcgccgccg ccgttctgct aggaggcacc acaggcccag agatactcgg
     5341 aagctacagc accacgtggc ccgataccga ggctgtcatg ggttgggatc tcgtcgagac
     5401 gggcctgaaa gtacagctct ccaagagtat cccgaacatc gtgcgcgata agttccgcga
     5461 caacctggag gaagcctgta attctctagg cctcgagttc gacgagctcg aacatttcgt
     5521 gctccatccc ggcggctcta gagtgctcga cgccttcgaa ggggctctcg gactgcaagc
     5581 gggcgggctt accaccagcc gagacgtgct gcgcgactgc ggcaacatgt cgagcgtcac
     5641 tgtactcttt atcctggaac gcttcctcga tgagggcgag ctcgcgaagg acgacctggg
     5701 tgtgctctcg gccttgggcc ccggattctc cgcggagcat gtgtttttca gatgctgacg
     5761 gccctcggcc gtcagcatct gatctgtcag cacgcgccct gtcgggcgct tgtcagcatt
     5821 tcagctagtc agcattttgt ggtggggtgg agggttcgtt gaatgttgcg gtgctggttg
     5881 cgggggtggc gctcgttgcg gtccagcgtt tgctggagct cacttactcg cggaggaatg
     5941 agcggaggct tcggtcccat ggtgcggtgg agcgtggggc cggccacttt cccgtgatag
     6001 tcgccatcca tactctgtgg ctggtctcga cgctcgtcga gggtcttctg cgcgggcccg
     6061 aacctcctgt gtggtggccc gtgccgctgg cggcgttctt gctagtccag ccgttgcggt
     6121 actgggcgat cttctctctg ggagtgaact ggaacgtaag agtactggtc gtgccaggcg
     6181 gaaaactcgt caggagggga ccataccgct acttccctca tcccaactac gttgtcgtcg
     6241 tggtggaggt cctgactttc ccactcatct tcggcgcctg gataacagcg atcgtctttt
     6301 cgctgctgaa cgccgccttg ctctacgtcc gtatccgaac agagaaccgg gcgctaagag
     6361 agctatcaag ttagaattga actggtcagc ctaacagcta gccgcccggc ttcgccaggc
     6421 ggcttgtcag cattacagcc aggctgtgga tggcgagagg aaagatctct cgagaagcgt
     6481 ttctagcgat gatcgatccg cagccgcgca caggtgacga acggtgatgc tgaagtgctg
     6541 accagcggag gccgtaggcc gaagcgtgct gacaagctga cgaccggagg gagacggcgt
     6601 gctagatctc aaatacatcc gcgagaacgc gaaagccgtc caggagaaca cgaggaaccg
     6661 tggcgtcgag gccgacgtgg gcctggttgt ggagctggcc gaccggcgct cggatctcat
     6721 acaggagtcg aacgagctca agcaacgcca gaaccagatg gccaggagca tcggcaggga
     6781 gcgtgacgag gaggctcggg cgaggctcat cgaggagtca cgggccatca aggagcggct
     6841 gccgtccaaa gaaagggagc tgcacgaggt cgaggagcgg ttgcgggacg agcagtcgaa
     6901 gatcccgaac ttgacgcatc ccgactcgcc tatcggcaag gacgacacgg agaacgtcga
     6961 gatccggcgt tggggtgatg ttcccgcctt cccgttcgag ccgaaggacc acgtggagct
     7021 tggcgagcag ctcggcatca tagacttcga cgccggggcc aagaccacgg ggaccaagtt
     7081 ctactttctg cgcggcgacg ccgtgctact ggagcttggt ctgatccgct acgcgatgga
     7141 catccttata gagcgcgggt accagccgac cataacgcct gacctggcgc gggatcggat
     7201 gctggtcggg acagggttca taccgcgcgg gccggagacc cagatctact ccgtcgaagg
     7261 tatggacctt tccctggtcg ccaccgcgga gatcccgctc gcaggccagc acgcagacga
     7321 gataatcgag gagagagacc tccccctgcg ctacgcaggc ctctcccact gcttccgcac
     7381 ggaggctggc tcccacggcc gcgccagccg gggcctctac agggtccacc agttcactaa
     7441 ggtcgagatg ttcgcgttca ccacgccgga tcagtcggag gatatgcacg acgagatggt
     7501 cgggatagag gagcgtatct tccagggcct cggcctgccc taccgcgtgg tcgacatctg
     7561 tacgggcgac ctgggcggcg ccgcctaccg gaagtatgac ctggaggcct ggatgcctgg
     7621 ccgcggcgat ttcggcgagg tgacaagtac gtcgaacacc accgactacc aggcccgcag
     7681 gctggccatc cgctaccgca aagaaggcgg aagaccccaa cttctgcaca ccctaaacgg
     7741 cacggccctc gctatcagcc gagccctgat ctccctcctc gagatctacc agcaacagga
     7801 cggctcggtc atgctgcccg aggtcctggt cccctatgtg ggcagggaac gcctgacgtc
     7861 ctcgtaaagc tgacaagctg gtagcttgca atatactctc ttgctggtcc gattttcatc
     7921 aggagaggag cgtcacgatg acagaagcgg tcgagaagct ggtcaagaag agtgaggacc
     7981 cgagcaggtg gtatttgcag ctcgtgcgga tggccaagct cgccgattac ggaccagtgc
     8041 gtgggacgtt cgcgatccgg ccctacgggt tcgagatctg ggagcgcgtc cagcgcgacc
     8101 tcgacgaccg cttcaaggag acaggccaca agaacgccta cttcccgctg ctgatccccg
     8161 agagctacct caggaaggag gccgagcacg tcgagggctt cgaccccgag ctcgcgtggg
     8221 taaccatagg aggaagagaa gagcttgagg agcggcttgc gatccgccct acctcagaga
     8281 gcatcatctg cgacttctac aggaactgga tccagagcta cagggacctg cccatcctca
     8341 taaaccagtg gtgtaacgtc ctgcgctggg agaaggtcac ccgcccgttc ctcaggaccg
     8401 cggagttcct ctggcaggag ggccacaccg tccacgcgac ggcggaggag gcgcgtgagg
     8461 agaccttgcg gatgcttggc gtctaccgcg attgcttcta tgaaaccatg gctattcccg
     8521 tgctcacggg cgccaagagc ccatccgagc gctttgccgg ggctgtggag acctacacct
     8581 gcgagggttt gatgggtgac ggccgtgccc tgcaggccgc gaccagccac gacctcgggc
     8641 agaacttcgc ccgcgccttc gacataacct tcctcgacga gaaccaggag agggtccatc
     8701 cttaccagac ctcgtggggg ttttcgacgc ggacaatagg gggtttgatc ctggtccatg
     8761 gcgacgacag gggcctcagg ttgccgccga ggatcgcacc gactcaggct gttatcgtcc
     8821 cgatctggcg tggcaagaac aagggagagg tacgccgcga ggctgaaact ctgcatgcgg
     8881 aactgaaaga ctccggcatc aggatggagg tcgacctcga cgaggagcac tcacctggtt
     8941 ggaagttcaa cgagcacgag ctcaggggcg tgcccgtgcg ggtggaactc ggacccaggg
     9001 acatcgagaa ggatcaggcc gtcctcgtca ggcgcgatac gggggagaag gagttcgtgg
     9061 acagaaagga gttgcccgac cggctagagg cggtgatggg cgagatccag gataacatgc
     9121 tgcgtcaggc gacggagttc cgcgatgcga acattcgcag ggccgaaagc tacgaggagt
     9181 tcgaggagat catcgccaga gagcgcggtt tcgtcgtcgc cccttgggac ggcacggagg
     9241 agaccgagca gaagatcaag gatgatacca aggccacgat ccggcttctg cccttcgagc
     9301 gggaggaggg gaaggatctc gtctcgggaa ggccaggccg taccgccctc ttcgcccggg
     9361 cttactgagg tcggtgcctg ttccgctcac cgatacgttg actccaggat ggcgagcggt
     9421 tagcatttgc gcattacgca acataacgta ggcgcataac tctaacgcgc ctgcctggag
     9481 ggggtctccg tgcggtcttc cgtactatgc gctgcgagat ggtgtctgac ttctgtcaga
     9541 gacagaggtg gctggcgagc accgcttggg tttctcgcgc tcgtgcctgc tctgatcctg
     9601 ctggccggag atcacgtcgc cgtcgaggcc gaggtgtact cctcgtactc ggatgccaag
     9661 aagcccgtca ttgctgtcat cctctccgag cagcagaatg tcgatgagtt caaggcccat
     9721 ttcggtctca gcgccaggca ggtcgacgcc gtcctcaccg cgacccggca cgagaaccgg
     9781 gtcctggcac aggtgtttgg tgagagcgag atcgtgctcg ccgcgaacag agatctcccc
     9841 aagaaagaga tagcgaagaa gatcgccgcc tccgactacg acgagaaggt cgccgcggcc
     9901 gtagcaagga cgaaacagag aatcgaggcc atcgtgcccg aggatcagag cgccgacctc
     9961 gagggctggg tggacgagca gtggaaccag gaggttctgg ccgcttccga ggaagactac
    10021 acggcctcct ccgagaccta catggtctcc agtcaaagga aaggcatacg gtgcagagtc
    10081 ttcgccacgc agtacaccgg gtacactgaa cgcgaggttg ccctgccgca tcgcaacctc
    10141 aaaatcggcc gccaaccgag ggttcgcatc tggcgcgtca gcggcgggcc cagcgtgggg
    10201 ccgagggtca aggaggtagg gccttggaac acctacgata attactgggt gcagaataag
    10261 gaccgcacga tgtacaaacg cgttccgcgt tgcaagccag cggcgcaggt cgcctactac
    10321 cagaacttca acaagggcaa ggatgagttc ggcagggaag tcctcaaccc cgcaggagtc
    10381 gacctgacac cggacgtcgc ccgtagcatg ggcctggagg aataccagaa tgcctgggtg
    10441 tgggtacgct ttccctgggt ccaccggtag gaagggtccc gtacctttct ctggaaggcg
    10501 cgactttctt gacgggtgca ccctcgtgtt ttagcatcaa cgcctagcgc gaacccgcat
    10561 cgagagcggt ggagggaact ggccccgaga aaccgcggca acccacgggg tgctgtgagc
    10621 tactcccgca aggtgccaag tccagcagga cggtcattac cgttctggaa gatgtgggga
    10681 gaggtgggca gcctcttcta cgagcgggtt gcgcgagagg cgtagaaccc gatcacagaa
    10741 ggaggccgca tt
//
LOCUS       DASTKM010000038         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_534692, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000038 DASTKM010000000
VERSION     DASTKM010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534692"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..663)
                     /locus_tag="VFQ10_01465"
     CDS             complement(130..663)
                     /locus_tag="VFQ10_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964037.1"
                     /translation="MYRAAALILVLVAVVFFATQLAEEPVRALQKPEVRPAPKVFEPA
                     AGAVGPGPDGGDLKIGDFEAPERVPDYEVLEKSLDTRDGARAVRLLIDTRSREEEHFV
                     LIARDLKSRYSDYDAVSVEFTDTEDLLFYNDDPQTRDLLAYYGGALIFNTYDGALYLG
                     YIYGPPNLDGYYVKAAD"
     gene            complement(681..1442)
                     /locus_tag="VFQ10_01470"
     CDS             complement(681..1442)
                     /locus_tag="VFQ10_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HET9964038.1"
                     /translation="MIPSLEDLLLVDLGCLILHEAHDEDRLARLRERIEAEREQRNPV
                     IVSPHEDRYLVLDGAHRVRALGELGSRFALVQAVEPPEKAEGWGHLLNGVGRADLDNI
                     EGIEVSHGPGDAPLAKVETAGGETLLLSAKEDGLPGRVRALWDLQSFYPKGVVVRRVE
                     PNGAARLSDGEALISYRSFTPRDLAEIVDSGTVLPAGITRFRVRERVLGVRYPLDRMM
                     EGDPRARNAELREFVEGRWKENRIRYYGEPVVLFE"
     gene            complement(1628..1701)
                     /locus_tag="VFQ10_01475"
     tRNA            complement(1628..1701)
                     /locus_tag="VFQ10_01475"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1665..1667),aa:Pro,seq:ggg)
     gene            1762..2172
                     /locus_tag="VFQ10_01480"
     CDS             1762..2172
                     /locus_tag="VFQ10_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET9964039.1"
                     /translation="MALAASYIRTESEGGLPTEDEQMASIVAYAADNGHDLVAHYKDL
                     DAPGLLLYHRPGLKEAIYNIKELEEWEVLIVAEPRCVSNTDSALHEFVHKLSLYGNRL
                     ESPARPWEGLLADMRSYRRAMRRGPSRETRDGVR"
     gene            2182..2550
                     /locus_tag="VFQ10_01485"
     CDS             2182..2550
                     /locus_tag="VFQ10_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964040.1"
                     /translation="MSFILTAIGFLLLVAALAGIGIGIYMAVEPKTRASGKLFAIWWV
                     PGLAAASGVLMRDVVTFTVGLICFSIAGAVMVFEDRRPRKSHVRGKGDLSRGPGGDIR
                     LDSEKTTRENRARDSGRAAS"
     gene            2722..2997
                     /locus_tag="VFQ10_01490"
     CDS             2722..2997
                     /locus_tag="VFQ10_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964041.1"
                     /translation="MGLVRTILVMVILLILTHVALKYSGVGPGANVLTNAVYSLGTLL
                     ESPAAFLLQSITILQNYLNPNSFYVVAISAAALYFIIYLLLGVGRRA"
BASE COUNT          639 a          982 c          985 g          613 t
ORIGIN      
        1 tttccgtccg gctgcatcct gtatgatatg cgctttccgg aaacccttca actgcgagga
       61 ccccgcaagc agcgtttttc aacacgtctc gccagatctg gcgagatttg tgggccatcg
      121 catgaggcgc tagtcggcgg ccttcacgta gtaaccatcc aagttgggcg ggccgtagat
      181 gtagcccagg tatagggcgc cgtcgtaggt attgaagatg agcgcgccac cgtagtaggc
      241 gagaaggtct ctggtttgcg ggtcgtcgtt gtagaagagc aagtcttcgg tgtcggtgaa
      301 ctccaccgag acggcgtcat agtcggagta gcgggacttc aggtcgcggg cgatgaggac
      361 gaaatgctcc tcctcacgcg agcgggtgtc gatgaggagc cgcaccgctc gcgcgccatc
      421 gcgcgtatcg aggctcttct cgagtacctc gtagtctgga acgcgctccg gcgcctcgaa
      481 gtccccgatc ttcagatccc ctccatcggg gccgggcccg accgctcctg ctgccggctc
      541 gaagaccttg ggcgccgggc gcacctcggg cttctggagg gccctcaccg gctcctcggc
      601 tagctgtgtc gcgaagaaga ccacggcgac gaggaccagg atcagggccg ctgcacggta
      661 caattaggac actccttctc tcactcgaag agtacgacgg gctctccata gtaacgtatg
      721 cggttctcct tccagcggcc ctcgacgaac tccctgagct cggcgttgcg ggcgcggggg
      781 tcaccctcca tcatcctatc cagcgggtaa cgtacgccca ggacccgttc ccgaacacgg
      841 aaccgggtta tcccggcggg caacacggtg cctgagtcaa cgatctcggc aagatctctg
      901 ggggtaaacg acctgtaact tatcagggcc tcgccatccg agagacgggc cgccccattg
      961 ggttccacgc gccgcaccac gacgcccttg gggtagaacg attgcagatc ccagagcgcc
     1021 cgaacccggc ctggcaaccc atcctcttta gccgagagga gtagggtctc gcctccggcg
     1081 gtctccacct tggccagggg agcatcgccg ggcccatggg acacttctat tccttcgatg
     1141 ttgtccaggt cggcccttcc gactccgttc aggaggtggc cccagccctc cgccttctcc
     1201 ggcggctcga ccgcctggac gagcgcgaag cgggaaccaa gctctccgag ggcgcgtacc
     1261 ctgtgggctc cgtcgaggac caggtaacgg tcttcgtgcg gggagactat tacggggttg
     1321 cgctgctcgc gttcggcttc gattcgttcc cgcagacggg ccaggcggtc ttcgtcgtgg
     1381 gcttcatgta ggataaggca cccgaggtct acaaggagaa ggtcttcgag ggaggggatc
     1441 acgtaccagg taagtagcgg gacgagcccg ccaggtctct aggggtctcc cccgcggcgg
     1501 ccccacaaaa agtcgagcaa gacgaaaccg ccgaagaaga agaccgcgat gatgatgaag
     1561 aatcccagga tgccaacaag aagatagatt gtgtccaaaa ctcgcctttc aacagaagcg
     1621 gcaatagtcg ggacggccgg atttgaaccg gcgaccactc ggcccccagc cgagtgcgct
     1681 accagactgc gccacgtccc gacgcgtctc tgactagacc tggatcatac cagacgagac
     1741 ccgtgctatc atgcgatctc cgtggcgctg gctgcatcgt acatacgcac agaatccgag
     1801 ggcgggctgc cgacagagga cgagcagatg gcctccattg tggcctacgc cgccgacaac
     1861 ggtcacgatc tcgtcgccca ctacaaagac cttgatgccc ctggtctcct gctctaccac
     1921 cgccccggtc tcaaggaggc gatatacaac ataaaggagc tggaggagtg ggaggtgctc
     1981 atagtcgcgg aaccaaggtg cgtctcgaac acagattcgg ccctgcacga gttcgtgcac
     2041 aaactctccc tgtacgggaa ccgcctggag tctccggcac gtccctggga gggcctcctc
     2101 gcggatatga gatcctaccg ccgcgcaatg cgtaggggcc cttcgcgtga aacgcgcgac
     2161 ggcgtccgat agtatagaaa ggtgagtttc attctgaccg ctatcgggtt cctcttgctc
     2221 gtggccgcct tggccggcat cgggatcggc atctacatgg cggtcgagcc gaaaacccga
     2281 gcatcaggca agctcttcgc catctggtgg gtgccggggt tggcagcggc gagcggcgtt
     2341 ctgatgcgcg atgtcgtcac gttcacggtt ggattgatct gcttctcgat cgcaggagcc
     2401 gtgatggtct tcgaggatag gagaccgcgc aagtcccacg tgaggggaaa gggcgacctg
     2461 tccagaggac cagggggcga catacgcctg gactccgaga agaccaccag ggagaacagg
     2521 gccagggatt ccgggagagc cgcctcctga cccgcctcgg cgcctctccc aggtaacagc
     2581 gttattgcta tactggccgc tgagaagggc cctgaggagg gtcatgggga ggagagagtc
     2641 cggtgcgata gaggccgcac gaacccctcg caaccatccg gggcgtaaca gtgttggtcg
     2701 cgaagggaag gagctagagg catgggtctc gtccgcacca tactcgtcat ggtcatcctg
     2761 ctgatactca cccatgtagc gctgaagtac tcgggcgtcg gacccggtgc caacgttctg
     2821 accaacgccg tctacagcct cgggacattg ctcgagtctc ctgccgcgtt cctgctccaa
     2881 tcgataacga tcctgcaaaa ctacctcaat cccaatagct tctacgtagt ggccatctcg
     2941 gccgccgcgc tctatttcat catctacttg ttgctcggtg tcggccgccg ggcctagaga
     3001 agcgtgcaaa gtggttcaac ctctttgcac actatcagct tgctagcatt gccttttcgc
     3061 cgatagccga tctcgcgtag ccgaatgagc gattcgctgt aggtcgggtc agttctggcg
     3121 gtgaaccggg gtgactacaa taacgtatac ttttccgagc gcttaccgaa tgattgggag
     3181 aagagccatg atcgaaacag taaagataag tgaatacag
//
LOCUS       DASTKM010000039         9469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_534729, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000039 DASTKM010000000
VERSION     DASTKM010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9469
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534729"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VFQ10_01495"
     CDS             <1..822
                     /locus_tag="VFQ10_01495"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9964042.1"
                     /translation="GERFHRAGGVPAVVNELLQAGKIEGGAMTVTGRSLAESVAGRES
                     KDREMITAHDEPLQERAGFRVLSGNLFDFAIIKTSVISDEFRERYLGDPANKDVFEGR
                     AVVFDGSDDYHERINDPDLDIDERSILVIRGAGPVGWPGSAEVVNMQPPDSLLKRGIN
                     TLPTIGDGRQSGTSDSPSILNAAPESAAGGGLAWVRTGDTIRIDLGSGRCDMLVSEEE
                     IERRKREEGIPDFPESQTPWQEIYRASVDQLDGGGVLESALKYRGIASKTPRHNH"
     gene            872..2308
                     /locus_tag="VFQ10_01500"
     CDS             872..2308
                     /locus_tag="VFQ10_01500"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HET9964043.1"
                     /translation="MNEPPGRVEAREYLVSQPPELRVPNALERIRRKIGESGRRLAVL
                     DDDPTGTQTVHGVPVLTTWSVEDLRWALEQPSSTFYILTNSRSFPEEEAARMNREISA
                     NLAAAAEQAGAKFAMVSRGDSTLRGYFPAETEALEEALRVDLDGIVLCPCYIEAGRLT
                     VEDIHWVRQGQSLVPAGETEFAGDASFGYSSSNLAAWVEEKTTGRVRASEVASVGLAD
                     IREGGPGRVAEMLLNVIGGRPVVVNAASYADLEVFVLGLLDAEEKGKRFLYRTGPSFV
                     RVRGGISEKGPLSAGELYEHRPRQGHGLVLVGSHVEMTTRQLEHALALSDVRPVELSV
                     PRLLDAGARDGELDRVVSEVDQGLAEAEVVVYTSREVVRGGAGRTGLEIGASVSNALV
                     EVMRRVDRRTPLAFVVAKGGITSSDVGTRGLGVRRAEVAGQMLPGIISVWILPEDSAF
                     PGLPYVIFAGNVGEEDSLARIVEILREG"
     gene            2309..3217
                     /locus_tag="VFQ10_01505"
     CDS             2309..3217
                     /locus_tag="VFQ10_01505"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9964044.1"
                     /translation="MLRAGFIGLGAMGAPMAKRIVSAGFDLSVFDVRPENADPLVDLG
                     ATRAGSPREVAEGAEALILMVVNAEQAENALFGGDGAAETLSPKSAVVLMSTIGPEAV
                     RGIEGRLAGRGAELLDAPVSGGVARAERGDLLIMAGGPADLFEEVRPLLAAMGSTVAH
                     CGGSVGDGQSVKLVNQLLCGVHIAAAGEALAYAEALGLDPRSVFETIRHGAANSFMLE
                     DRGERMLGREFVPAKSALGIFVKDMGLVRLAAGEQGFRTPLADAAHRLYQVGSSLGLG
                     EEDDSGVVRVFENADGAGRAGVSLQI"
     gene            3283..4038
                     /locus_tag="VFQ10_01510"
     CDS             3283..4038
                     /locus_tag="VFQ10_01510"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET9964045.1"
                     /translation="MAFSEEVAWVTGSSTGIGRAVAVALAGQGRRVVVHYNASEDEAR
                     EVVEEIEASGGEASIVGGDVSDADEVGRMVGEIEDRFGRLDVLVNNAGSLIERRSLSE
                     MTDDLWDRVMDVNLKSVYLVSRAVLPIMRRQGRGRIINMTSIAARNGGGPGSVAYATA
                     KGGVSTLTRAMAKELVSENILVNGVAPGVITTPFHDRFTPPDVRETLKNTIPMGREGT
                     PEETAGVVAFLTSPAADYLVGEIIEVNGGQLMS"
     gene            complement(4163..5113)
                     /locus_tag="VFQ10_01515"
     CDS             complement(4163..5113)
                     /locus_tag="VFQ10_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9964046.1"
                     /translation="MKNRVVDPSSRRTLGGTGLSVSPLCVGCAPLGDMPETFDYSVDE
                     DRARETLRAVLDSQINFLDTAASYGDGESERRIGGMLEEMGGLPEGVVLATKADRDLT
                     TGDFSGEQMRRSVERSLWLLGMDRLQLVYLHDPEHESFDHMMSRGGPVEVLTGLKEEG
                     VIQHLGVAGGPIDLMIRFVETDLFEAVITHNRYTLVNRTADPLFDVAEERGVAALNAA
                     PYGSGILAKGPDAYARYEYREAPKEMVERVRAIQDACREFDVPLTAAALQFSLKDPRI
                     ISTIVGMSRPERVKQTLDLATRPIPPELWERIDALASPNG"
     gene            complement(5131..5949)
                     /locus_tag="VFQ10_01520"
     CDS             complement(5131..5949)
                     /locus_tag="VFQ10_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9964047.1"
                     /translation="MKLLLYRADFMQDKPSSWEALLESATRLRSDNIYGFAFPGKESG
                     LFGHFFELQAMAGGTMFEDEGPPAPRLDDEAGRWALGLLKDLYERASPPETPDWHYDE
                     VAACFREGRAAMSTDWPGGFYTYLDTDQSAVVDRFDVALYPEGPAGRHIYSSSHTFAI
                     PATVSDRPAAIELLRFLTSRESQAHEARLGTLPARSDSLQDVRSEAGPFAERRWNLLE
                     RTQEAALVPPKHANYPAVEDAIWEGIREALLGNKSVEKALEDTEAAARRAAEGV"
     gene            complement(5957..6286)
                     /locus_tag="VFQ10_01525"
     CDS             complement(5957..6286)
                     /locus_tag="VFQ10_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964048.1"
                     /translation="MSRSLKALLVGGPMYDPLYTRLAEFEVIQDLRVETVVAPTHPDL
                     NEQIEAEFASGGASYDLISTHTKYAPSQKQWLTPLDDDLDEGELEPFTPRTLELAHRR
                     ESLQRPA"
     gene            complement(6283..6702)
                     /locus_tag="VFQ10_01530"
     CDS             complement(6283..6702)
                     /locus_tag="VFQ10_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9964049.1"
                     /translation="MACLESHRPLDLITLMLGTNDLKARFGSSASDIAQGAASLAEMM
                     LRSGCGPDGGAPVVVLISPPAVATLTDMAQMFEGAQEKSRQFSRHYERFAEQHGCEFF
                     DASEVIVSSDVDGIHLDPGEHRKLGEAVAARIGEILG"
     gene            complement(6702..6911)
                     /locus_tag="VFQ10_01535"
     CDS             complement(6702..6911)
                     /locus_tag="VFQ10_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964050.1"
                     /translation="MRTILCYGDSNTWGSDPETRERFPEDVRWPGVLRKRLDEEYRVI
                     EEGLPGRTTVRDDPIEGITRTAGLT"
     gene            7119..9155
                     /locus_tag="VFQ10_01540"
     CDS             7119..9155
                     /locus_tag="VFQ10_01540"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HET9964051.1"
                     /translation="MHERRYRQVHLDFHTSADCEGVGADFDPEVFANTVRMGRVKSMT
                     VFAKCHHGFSYYPTEVGTRHPNLAVPDLMGAQIEALHGIGVLAPIYISVMWDDLAGEK
                     EPGWIIARRDGSLMIRPPLSNESPLTGGRGWTTLDVSTGYGDYLVAQTEEICDRYDVD
                     GFFFDICFPVPNYTPWGQARMRETGVRPDEEQSVLRFAEEKLKGFLDRLSEVVRTKVP
                     QATIFYNGTVNPRMAETLPYQTHFEIESLPTSSGIWGYLHYPVMARQARTYGVDFLGM
                     TGRFHKAWADFGGLKTRDQLDYECGTVVGAGGGISVGDQLHPRGVLDPAVYRLLEHSF
                     GRVERLEPFLKGAKPTAEVAILSGYEPSSRGSIPTNTHVADVEGAAQFFLEAAIQFDI
                     VDPSHGSFENYAALLLPDGMSVNHDLRANLERYMSAGGKLILGGTAALARETGEFGIK
                     GVPVAYEGQAPTVPCYLRLDETLAGETELATDYDYVFYNRAHLVRPLEGARAHGELRR
                     ALFDRTWEHFTSHAQAPVGESLDSPLVVEGDGVLYFAAPLFGAYRDHDYWVYREVARN
                     AIRGFLPRPKLKVSGPGWVEATLHEQPAGYDHPKRRILHLVAYHPRRTTQPVQHVDQS
                     WPTSGLGVDVLTDGRTIGRVYLAPGGQPLDFEEVDGYARIALPPVGAHTVVVVE"
BASE COUNT         1713 a         2900 c         3161 g         1695 t
ORIGIN      
        1 ggagagaggt tccaccgtgc tggtggcgtg cccgcagtgg tcaacgagct tctgcaggct
       61 ggcaagatcg agggcggggc gatgaccgtc acgggcaggt cgctggccga gagcgtcgca
      121 gggcgcgaga gcaaggaccg agagatgatc acagcccacg acgagccgct tcaggagcgc
      181 gctggttttc gggtcctctc cggcaacctc ttcgacttcg ctatcatcaa aacgagtgtg
      241 atctcagacg agttccgcga acgttacctg ggcgatcccg ccaacaagga cgtgttcgag
      301 gggcgggcgg tagtcttcga cggctcggat gactaccacg agcgtataaa cgaccccgac
      361 ctcgacatcg acgagcgctc tattctggtg atccgtggcg ctggaccagt cgggtggcct
      421 ggctcggccg aggtcgtcaa catgcagccc cctgactcgc tcctcaagcg tgggatcaac
      481 accctgccta ccatcggcga cggcaggcaa tctggcacct cggacagccc ttcgatcctg
      541 aacgccgcac cggagagcgc cgcaggaggc ggcctggcct gggtgcggac gggggatacc
      601 atccgcatcg acctgggtag cggacgctgc gacatgctgg tctccgagga ggagatagaa
      661 cgccgcaagc gagaggaagg catccctgac ttccccgaga gccagacgcc ctggcaagag
      721 atctaccgcg cctccgtgga tcaactcgac ggcggcgggg tactcgaaag cgcgctgaag
      781 taccgtggca tcgcctcgaa gaccccccgc cacaaccact gatggaaagt tcgttaaacg
      841 ccaagacgac gcgagtgcga ggcgttcttc gttgaatgag ccgcctggac gagtcgaggc
      901 aagggagtac ctggtctcgc agccgcccga actgcgtgtg ccaaacgccc tggagaggat
      961 caggcgcaag ataggcgaga gcggcaggcg ccttgcggta ctcgacgacg acccgacggg
     1021 cacccagacc gtccacggcg tccccgtcct gacgacgtgg tctgtcgaag acctgcgatg
     1081 ggcgctggag caaccctcct ccacgttcta tatccttacc aactcgcgca gtttcccgga
     1141 agaggaagcc gccaggatga accgcgagat ctcggccaac ctcgcggcgg cggcagaaca
     1201 agctggtgcg aagttcgcca tggtaagccg cggcgactcc accctgcgcg gatacttccc
     1261 tgccgagacc gaggcattgg aggaggcact cagggtagac ttggacggca tcgtcctgtg
     1321 tccctgctac atcgaggcgg gccgcctcac tgtggaggac atccactggg tacgccaggg
     1381 ccagagcctt gtgccagcgg gagagacgga gttcgccggc gatgccagct tcggctactc
     1441 ttcctcgaac ctcgccgcgt gggtcgagga gaagacgaca ggccgcgtgc gggcctctga
     1501 ggtcgcgagc gtggggctcg cggatatcag ggagggcggg ccagggcggg tcgccgagat
     1561 gctactcaac gtaatcggcg gcagacccgt cgtggtcaac gcggcctcgt acgccgacct
     1621 cgaggtcttc gtcctcggcc tgctcgacgc ggaggagaag ggcaagcgtt tcctctaccg
     1681 tacggggcct tctttcgtgc gggtacgcgg cggtatctca gagaaaggac ccttgagtgc
     1741 gggggagttg tatgagcatc gcccgcggca ggggcatggg ctcgtgctgg tcggttccca
     1801 cgtcgagatg accacgcgcc agttggaaca cgcgctcgcg ctttcggacg tacggcctgt
     1861 cgagctctcc gtgccgcggt tgctcgacgc gggcgcgcga gatggggagt tggatagggt
     1921 ggtttcggag gtcgaccagg ggttggccga ggctgaagtc gtagtttaca cgagcagaga
     1981 ggtcgtacgc ggcggtgcgg gcaggacggg gctggagatc ggggcctcgg tctccaatgc
     2041 gcttgtggag gtgatgcgga gggtggacag aaggacgccc ttggcattcg tggtggccaa
     2101 gggaggcatc acatcgagcg acgttggcac ccgcggtctc ggcgtacgta gggccgaggt
     2161 cgccggccag atgctaccag gcataatatc cgtctggatc ctgcctgagg acagcgcctt
     2221 tcccgggcta ccctatgtca tcttcgccgg caacgtaggc gaggaggact cgctggcaag
     2281 gatcgtcgag atactaaggg agggataaat gctacgcgcc ggattcatag gactcggggc
     2341 gatgggcgcc ccgatggcca agaggatcgt ctccgccggt ttcgaccttt cggtcttcga
     2401 cgtacgcccc gagaatgctg atcccctggt cgacctgggg gccacgcgtg cgggctcgcc
     2461 gcgagaggtg gccgagggag ccgaagcgct catcctgatg gtggtgaacg cggagcaggc
     2521 cgaaaacgct ctgttcggag gagacggcgc agccgagacc ctgtctccta aatccgccgt
     2581 tgtactcatg agcacgatcg ggcccgaggc ggttcgcggg atcgaagggc gattggccgg
     2641 gcgcggcgca gagttgctcg acgcgcccgt cagcggcggc gtggcgcggg ccgagcgtgg
     2701 agacttgctc atcatggccg gcggtcccgc tgacctgttc gaggaggtca ggcccctgct
     2761 cgcggcgatg gggtcgacgg ttgcgcactg cggcggatcc gtgggggacg ggcagtccgt
     2821 aaagctggtc aaccaactgc tctgcggggt gcacatcgcg gcggcgggag aggcgctcgc
     2881 ttacgcggag gcactgggcc tcgatcccag gtcggtgttc gagacgatca ggcacggtgc
     2941 ggccaactcg ttcatgctcg aggacagggg tgaacggatg ctcgggcgcg agttcgtccc
     3001 cgcgaagagc gccctcggca tcttcgtcaa ggacatgggg ctggtgcggt tggccgcggg
     3061 agagcaaggc ttcaggacgc cgcttgccga cgctgcgcac aggctatacc aggtgggatc
     3121 ctcgctcggg ctgggcgagg aggacgattc cggtgtcgtg cgcgtcttcg agaacgcgga
     3181 cggagcgggt cgagcaggcg tttctcttca gatctagctc atttgcacga tgtacgttct
     3241 atcctttgtt gaggagcaag gctacaggga ggtaacggtc ggatggcttt ctcggaagag
     3301 gtcgcgtggg tgacggggag cagcacgggg atcggacgcg ccgtcgccgt ggcgctcgcc
     3361 gggcaggggc gccgggtggt ggtccactac aacgcgagcg aagatgaggc gcgggaggtg
     3421 gtcgaggaga tcgaagcctc aggcggcgag gcttcgatcg tcgggggtga cgtctctgac
     3481 gccgatgagg tcgggcggat ggtcggggag atagaggacc gtttcgggcg gctcgacgtc
     3541 ctggtcaaca acgcggggtc actgatcgaa cgcagaagtc tatccgagat gaccgatgac
     3601 ctctgggatc gggtgatgga cgtgaacctg aagagcgtct acctggtctc gcgggctgtg
     3661 ttgccgatca tgaggcggca aggaagaggc aggatcatca atatgacgtc tatcgcggcc
     3721 cgaaacggcg gcggtcctgg ctcggtcgcc tacgccacgg ccaaaggcgg cgtaagcacc
     3781 cttacccgcg cgatggccaa ggaactcgtc tccgagaaca tcctcgtaaa cggggtcgcc
     3841 cctggcgtca taaccacccc tttccacgac cgtttcacgc cccccgacgt acgcgagacc
     3901 ctgaagaaca ccatccccat gggacgcgag ggcacccccg aggagacggc cggggtcgtc
     3961 gcgttcctca cttctccggc ggctgactac ctggtaggtg agatcatcga ggtcaacggc
     4021 ggccagttga tgagctagtc gcagaaagga gatccacacc tccccatgtt cggtgcataa
     4081 aaacaagaga aggggcaggc gacgcttgcg tgaagcacag gtagtgaacc tgagagtacc
     4141 atgagatgct ctgccggcca ccctaaccgt tcggggatgc cagcgcgtct atgcgctccc
     4201 acagttcggg tggtatcgga cgggtggcga ggtccagcgt ctgtttcact ctctcagggc
     4261 gactcatgcc gacgatggtg gagattatgc gggggtcttt cagtgagaat tgcaaggctg
     4321 cggctgtcag gggaacatcg aactcgcggc aggcatcctg tattgcgcgg acccgttcca
     4381 ccatctcttt cggtgcctcc ctgtattcgt agcgtgcgta ggcgtcgggg cctttggcga
     4441 ggatgccgct gccgtacggg gcggcgttga gcgctgccac tccgcgctcc tcggcaacgt
     4501 cgaagagagg gtctgcggtc ctgttcacga gcgtgtagcg gttgtgggtg atgacggcct
     4561 cgaagaggtc cgtctcgacg aaccggatca tgaggtctat cgggcccccc gcgactccga
     4621 ggtgctggat aactccttct tccttgaggc ctgtcagtac ctcgaccgga ccgccgcggg
     4681 acatcatgtg gtcaaagctc tcgtgctccg gatcgtgcag gtagaccaac tggagcctgt
     4741 ccatgcctag tagccacagg gaacgctcga cggagcggcg catctgctct ccggagaagt
     4801 ctccggtcgt gaggtcccta tccgccttgg tggccagtac gacgccctct ggcaggccgc
     4861 ccatctcctc gagcatcccg ccgatgcggc gctcgctctc tccgtcgccg tatgaggccg
     4921 ccgtgtcgag gaaattgatc tgactgtcca agactgccct cagcgtctcg cgggcccggt
     4981 cctcgtctac agaataatcg aaggtctcgg gcatgtcccc cagcggcgcg catcccacgc
     5041 acaacggcga gactgacaga cccgtgccgc ccagggtcct cctcgacgag gggtcgacga
     5101 ctctattctt caacattcct cccttgcgga ctacacgccc tctgccgccc gacgggccgc
     5161 ggcctctgta tcctccagcg ccttctccac gctcttgtta cccagaagcg cctccctgat
     5221 accctcccag atagcatcct cgaccgcagg atagttcgcg tgcttgggcg ggacgagcgc
     5281 agcctcctgc gtccgttcca gcagattcca acgccgttcg gcgaacgggc cggcctcgga
     5341 acggacgtct tgcaaggagt cgctccgtgc gggaagggtt cctagcctgg cctcgtgcgc
     5401 ctgcgactcc cgcgaggtca ggaagcgaag gagctcgata gccgccggac ggtcgcttac
     5461 ggtggcaggg atggcgaacg tgtgcgagct cgagtatatg tgacgcccgg ccggcccttc
     5521 ggggtagagt gctacgtcga agcggtccac gacggcgctt tgatcggtat cgaggtacgt
     5581 gtagaagccg ccgggccagt cggtgctcat ggcggccctg ccttcacgga agcaggcggc
     5641 tacctcgtca tagtgccagt ccggcgtctc agggggcgag gccctctcgt agaggtcctt
     5701 gagcagcccg agcgcccagc ggcctgcctc gtcgtccagg cgaggcgcgg gcggaccttc
     5761 atcctcgaac atcgtgcctc cggccatcgc ctgcagctcg aagaagtggc cgaagagtcc
     5821 tgactccttg ccggggaagg cgaagccgta gatgttgtcg gatctcagcc gcgtggcgga
     5881 ctcgagcagg gcctcccacg aggagggctt gtcctgcatg aagtcggcgc ggtacaggag
     5941 gagcttcacg tccaggttac gcgggacgct gaagagactc ccgtctatgc gccaactcta
     6001 acgtgcgggg agtgaagggc tccagttcgc cctcgtccag gtcgtcgtcg agaggggtta
     6061 gccactgttt ctggctcggg gcgtacttgg tgtgagtcga gatcaggtcg taagaggcgc
     6121 ctcctgatgc gaactcggct tcgatctgct cgttgaggtc agggtgtgtt ggggcgacta
     6181 ccgtctcgac cctcagatcc tggataacct cgaactccgc gagacgcgtg tacaggggat
     6241 cgtacatcgg acctccgacc agcaaagcct tcaagcttct actcatccta agatctctcc
     6301 tatgcgggct gcgacggcct ctccgagctt tctgtgctcg ccggggtcga ggtggatgcc
     6361 gtctacgtcg ctggagacga tgacctcgga ggcgtcgaag aattcgcacc cgtgctgctc
     6421 ggcgaagcgc tcgtagtgcc tggagaactg cctggatttc tcctgcgctc cctcgaacat
     6481 ctgcgccatg tcggtgagcg tggcgaccgc gggaggggag atcaaaacca caacaggagc
     6541 gccgccatcc ggcccgcacc cgctccttag catcatctcg gccaggctgg cggccccctg
     6601 cgcgatgtct gaggcggagc ttccgaagcg cgccttgagg tcgttggtcc ccagcatgag
     6661 cgttatgagg tcaagggggc ggtgcgactc gagacacgcc atcaggtaag tcctgccgtt
     6721 cttgtgatcc cctcgatggg atcgtccctc accgtcgtcc tgcccggcaa gccctcctcg
     6781 atcacacgat attcctcatc cagcctcttc ctgagcaccc caggccagcg cacgtcctcg
     6841 ggaaaccgct ccctcgtctc ggggtccgag ccccaggtgt tagagtcccc gtaacacagt
     6901 atcgttctca tcgattcccc tctcgcccca cagatactgg caccattgtt ggcgccgtgc
     6961 gtaggccggg tcaaccacag acctatgcaa aagggataga tactcgaagc ggatatgctt
     7021 gtttcttgac cgtgacgatc ccgccgggta acgtccgttg tgaggccgtg ggaacggaga
     7081 agtttccggt cgtcgaggtc tcccggagag gtagaaggat gcatgagcgg cgatacagac
     7141 aggtccacct cgatttccac acctcggcag actgcgaggg tgttggtgct gacttcgacc
     7201 ccgaggtctt cgccaatacc gttcggatgg ggcgggtaaa gtccatgacg gtcttcgcca
     7261 agtgccacca cggcttctca tactatccga cggaggtagg cacgaggcac cccaatttgg
     7321 ctgtcccgga tctcatgggc gcccagatag aggcgctgca cgggatcggg gtgctggccc
     7381 cgatctacat tagtgtcatg tgggacgacc tcgccggcga gaaggagccc ggctggatca
     7441 tagcccgcag ggacgggagc ctcatgatcc gtcccccgct ctcgaacgag tctcccctga
     7501 cagggggaag gggatggacg accctcgacg tctctaccgg atacggtgac tacctcgtag
     7561 cgcagacgga ggagatctgt gaccgctacg acgttgacgg cttcttcttc gacatctgct
     7621 tccccgtccc gaactacacc ccgtggggcc aggcaaggat gcgcgagacc ggagtgcgcc
     7681 cggacgagga gcagtcagta ctgcgttttg ccgaagagaa gctgaagggc ttcctcgacc
     7741 ggctgagcga ggtggtccgc acgaaggtcc cccaggccac catcttctac aacggcacgg
     7801 tcaacccaag gatggccgag acgctcccct accagaccca cttcgagata gagagcctgc
     7861 ccacctcgtc agggatctgg ggatacctgc actaccccgt catggcgcgg caggcccgca
     7921 cctacggggt cgacttcctc gggatgaccg ggcgcttcca taaggcctgg gccgacttcg
     7981 gcggcctgaa gacccgtgac cagctcgact acgagtgcgg caccgtcgtc ggcgcaggag
     8041 gtgggatctc cgtcggcgac cagctccacc cgagaggcgt cctcgacccc gccgtctacc
     8101 gcctgctcga gcattcgttc ggcagggtgg aaagactcga gcctttcctg aaaggggcga
     8161 agccaactgc ggaggtggcg atcctgtcag ggtacgagcc atcctcccgc ggaagcatcc
     8221 ccacaaacac ccacgtcgcc gacgttgaag gagccgcgca attcttcctg gaagcggcga
     8281 tccagttcga catagtagac ccctcgcacg gctccttcga gaactacgcg gccctactgc
     8341 tccccgacgg aatgagcgtg aaccatgacc ttcgcgcgaa cctggagagg tatatgtcag
     8401 ccggcggcaa acttatcctg ggcggtacgg cggccctcgc ccgagagact ggcgagttcg
     8461 gcatcaaggg cgtccctgtc gcctacgagg gacaggcccc gacggtacct tgttacctgc
     8521 ggctcgacga gacgctagct ggcgagaccg agctggcaac agactacgac tacgtgttct
     8581 acaacagggc gcacctggtt cgcccgctcg aaggcgccag ggcgcacggg gaactacgtc
     8641 gcgcgctctt cgaccggacg tgggagcact tcacgagcca cgcccaggcg ccggtcggcg
     8701 agtcgttgga ctctccgctg gtagtagagg gcgatggcgt tctctacttc gcagcgccgc
     8761 tcttcggggc ttaccgcgac cacgactact gggtctacag ggaggtcgcc aggaacgcga
     8821 tccgcggctt cctgccacgg ccgaagctga aggtatcagg gcctggatgg gtggaggcca
     8881 cgctgcacga gcagcctgcg ggctacgatc atccgaagcg gcggatcttg cacctcgtcg
     8941 cctaccatcc ccgccgcacc acgcagcccg tacagcacgt ggaccagtcg tggccgacct
     9001 cagggctggg cgtcgatgtc ctgacggacg ggagaactat aggacgggta tatctcgcgc
     9061 ccgggggcca gccgctcgac ttcgaggagg tggacggcta cgcgcgcata gcgctgccac
     9121 cggtcggagc gcacaccgtg gtggtagtgg agtagagaag tgcgtgccgg ggcacgcgaa
     9181 gcaccgtctc gatagaaggc gccggccaac gttgcttacg gtcggatgag tttgaaacgt
     9241 gttgcactat ccggtacaga gcttgtatcc tcctactatg gtgctaggtt agtgtaagct
     9301 ggtaatccga aggggagcaa gggaagggga cctgtggagc aaagacagga aagtacgagg
     9361 atcgatccca ccgtattctg ggtagcagct tttatttcgg cggtgttcgt cctgtggggc
     9421 attctcttta cggacaacct agccgccgtc ttcgatgcgg tgctatgga
//
LOCUS       DASTKM010000040        10043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_7188, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000040 DASTKM010000000
VERSION     DASTKM010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7188"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            653..1786
                     /gene="dnaN"
                     /locus_tag="VFQ10_01545"
     CDS             653..1786
                     /gene="dnaN"
                     /locus_tag="VFQ10_01545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET9964052.1"
                     /translation="MRAVCSTETFGKKLALVSRGVSARSTIQLLGGILLEGRGEALGL
                     SATDMEISIQTSSQAEVDGEGRVVIPARIFNDIVRSLPGGSFSLEHDDSEGTVRLVAG
                     ENEYRIRAYAADDYPRLPVFPEEGTFRMSGESLVETVEKVSRSYSRDETRPVLTGILI
                     SFEESRVRMVTTDSYRLSIKETELATTSFEGSREAIIPARAMQEVSRIFSSSDEDDVE
                     VALSENQALFRIGDVALGTRLIDGNFPEYRRLLPGGFEREISVSREELVGTLRRVNLF
                     AARQTPPVPVSLSFSEGSVEVIVRNGEVGDAHERLSASSEDEFLISFNPGYLLDGVSA
                     IDSENVVFKLNEPLKPGLIVPEENGEEPDFLYLIMPMRDPSRG"
     gene            1808..1984
                     /locus_tag="VFQ10_01550"
     CDS             1808..1984
                     /locus_tag="VFQ10_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET9964053.1"
                     /translation="MTLGQAIKASGLVGTGGEAKVLIQAGEVSVNGEVETRRGRRLEE
                     GDVVEVGDERLEIG"
     gene            1981..3105
                     /locus_tag="VFQ10_01555"
     CDS             1981..3105
                     /locus_tag="VFQ10_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET9964054.1"
                     /translation="MSAHLRALRLVNFRNYVDATALLAPGLNVVVGDNAQGKTNLLEA
                     IAFNVTGSSPRTPKENEVVRWGADFARTEARIALDGYTGERGVAIGYAPGQKKRITVD
                     GVPASSLTNYAAGGAGARVVTFFPDDIRVVKGAPADRREFLDALLSSLRPAYARAAVE
                     YARAVQQRNQLLRRIRDGFSSERTLATWDKKVVELGKTVLAGREAALPVLEEHFIDAL
                     RALYGPEKAAIRYGHSSTLEEYSEALREAHSADVERGTTSVGPHRDDFEVSLGGVNLT
                     TFGSQGQQRIATLALKFASRDYVRGAVGEDPILLFDDVMSELDERRREYLAGYFLEST
                     QAVISTTNLEYFDEEILRRTRIIRISGGSILETATDGARR"
     gene            3262..5199
                     /gene="gyrB"
                     /locus_tag="VFQ10_01560"
     CDS             3262..5199
                     /gene="gyrB"
                     /locus_tag="VFQ10_01560"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET9964055.1"
                     /translation="MAAKPTIKNAKSAYDASTIQVLEGLEAVKRRPGMYIGSTGPRGL
                     HHLVREIIDNSIDEALAGYCSEILVTIHPDNSVTVTDDGRGMPVGKMAKYGKPAAEVI
                     MTTLHAGGKFGGEGYKVSGGLHGVGASVVNALSEWLKLEIKVEGHVWSQRYERGFPKG
                     ALTKDRKLKKGEGTGTTVSFLPDPEVFTETQDFSFDTLSRWFREWAFLNKGLKIRLAD
                     QREEARDVTYQYEGGIRDFVEHINEAKDPVHKTIFYLEREEEVGDVEVAMQWNSGFQD
                     SVFTFANNINTHEGGAHLSGFRSALSRTINAYARQKGLLKEKEESLTGDDIREGLAAV
                     ISVKLREPQFEGQTKTKLGNTEVKGLVESVTNRYLAEFLEERPGEAKAIVNKALQAAR
                     ARLAARTVREKIRKGYLESSTLPGKLADCSSKDPARSELYIVEGNSAGGSAKGGRDRN
                     FQAILPLRGKILNVEKANINKILSNAEIQALISAIGAGVGESFDLEQARYNKIVIMAD
                     ADVDGSHIRTLVLTFLYRNMRDLIEAGYVYIALPPLYKITHNRREHYAKNDAELRETM
                     DRMRANGNASISRFKGLSEMNPQQLWETTMDPQNRTMLQVTVESAAVADELFTALMGD
                     KVEPRKQFIEENAREVKNLDA"
     gene            5192..7756
                     /gene="gyrA"
                     /locus_tag="VFQ10_01565"
     CDS             5192..7756
                     /gene="gyrA"
                     /locus_tag="VFQ10_01565"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET9964056.1"
                     /translation="MLDTFSGNVQPHSIEDELKDSFLSYAMSVIVSRALPDARDGLKP
                     VHRRVLYAMQDLGLQPNRPYRKSATVVGDVMGKYHPHGDQAIYDALARMAQDFALRYP
                     LVDGQGNFGSVDGDPPGAMRYTEARLTRMATEMLRDINADTVDFVPNFDESLKQPEVL
                     PARFPNLLVNGSDGIAVGMATKIPPHNLGEVIDATMALIDDPDLDDEEIAKYIKGPDF
                     PTGGLIVGLRGIRDAIATGRGSVRVQAKAHTEQIKGNRTQIVVTEIPYQVNKSYLLQK
                     IAELVKDRKLQDISDLRDESDRNGMRIVIELKREAVPKVVLNNLYKHTQMQQSFGVNL
                     VALVDGVPRTLSYRQALGHYIAHQFEVVTRRTRYELGRARARAHILEGLLIALGDLDE
                     VVATIRRSRSVETARKNLMKGFGLSERQAQAILDLRLQRLTAMERQKVQQEHRDLREK
                     IEYLEGILADEYKVYGIVKAELGEVRAAYADDRRTAITAEEGVDFEIEDLIAEEEMVI
                     SISNGGYLKSVPVNSFRKQGRGGVGVAGMELKEGDYIDHLFITTTHHYMLFFTNKGKV
                     YRLKVHQLPRMGRAAKGRHIANMLPLAQGEHIASVLATKEFEEAEYLLFATRRGIVKK
                     TRFLEYNTPLKNDGIIAINLAGDDELISVRYASEGDEVVIVSRNGKGIRFKQTDARPM
                     GRATAGVKSITLGNDDVVLSMDVISDDAADLFILTERGFGKRTALSQYRAQGRGGQGV
                     IAMKTDGERGKLAGVRVVRPGLHELMIVSNFGTTIRIDADSVSRQGRAAQGVRVMNLR
                     SGDSVSAIAKVISSRSADADGATDELTLEELSGDGQTGTSTAGLPEPEGIGAVPGE"
     gene            7749..8162
                     /locus_tag="VFQ10_01570"
     CDS             7749..8162
                     /locus_tag="VFQ10_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964057.1"
                     /translation="MNSECEGVVECTALVLAESCTEYEGRLTLFGVLDEIRPNGASGT
                     SFVVYTKFEGCEHVSDRERRARIIVTDEDGEVLMESAEYSVYLDGSEEPVTKVAAFDL
                     PEGFETGEQLLWIEAVLDEETLSQTVVRIHDRYSV"
     gene            8219..9406
                     /locus_tag="VFQ10_01575"
     CDS             8219..9406
                     /locus_tag="VFQ10_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9964058.1"
                     /translation="MPEDLAVFIDWENIYISTVTEYKSRPNVSAILEKAREYGRIVAA
                     TAYADWTDGDFRQAPPTLYSNGISPRYISARYFPGGKSQKRRTNSIDVMLAVECSDFL
                     HNHPQVDTYVLVTGDGDFIPLVNLLRSKGKNVVVIGVSEATSYHLIESADHFVSYASL
                     LEEDRAARTQDREPKKEAADPYKELVRAVQTLKKGGKTRVLGQVKQQMIVQLGSFDER
                     NAGFKKFKDFVVEAERRGLVNTVDQDLELQVYLPDEQVPDLQETDLATEEPSSQSQSA
                     SGNRGNGRPAREVRQQEPEPGFDLLEDLTPYELNTVCTSIASLEQPATFVEIFSSIRD
                     LDERGELELSREEIGDVITAMLDADYLGKVRQRPYKQAQADMTFYALNAEKQEVKSAL
                     NSG"
     gene            complement(9416..9697)
                     /locus_tag="VFQ10_01580"
     CDS             complement(9416..9697)
                     /locus_tag="VFQ10_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964059.1"
                     /translation="METMLDIGVGILRLIPLILAFYVPALLGTATWSEGDRTKAILWF
                     AIGFGAIVAVQVLFSSVSAIQVAGIVGLSLVQIAAALCLAALTVYKLAD"
     gene            9800..>10043
                     /locus_tag="VFQ10_01585"
     CDS             9800..>10043
                     /locus_tag="VFQ10_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964060.1"
                     /translation="MGINQRVSLRKFIDDIRRWVEEDRSDEWIASALGSSPSSVQSFR
                     SRNAIYRRGAASTLHDPQDYSSYEGVLEPEGPGIWFD"
BASE COUNT         2224 a         2959 c         3158 g         1702 t
ORIGIN      
        1 agatagggaa ggccttcggc ggacgcgacc attccaccgt tatgcacgcc acggcaaaga
       61 tagccaacct cataaacagc gaccgcgacg tcttctccca aatacatgag atcacccaac
      121 aggtcaagag taaaaggtga cagctaaccc cctcccccag ggataggaga ttgtaacacg
      181 aaatatgcat tgaatgtgta ttctatccac aacctatcca cagggcgtcg tcgtcttgtg
      241 gataagtcgt cggaaacctt ccacaaccag gtcaggttat ccacagtttc tgtggataac
      301 tcttccgacc tgtggataaa accggcggta acagagaaat tcaggcaggt tgcagcattt
      361 gatcctagag atgcctgtgg ataaacctgt ggataagagg ggtagaatct gtgggtaaac
      421 ctgtggatag aattgtggac aactccagcc tgtggataac tggccactta tccacaattc
      481 gtccacagga aaacgcgggt tatccacatt tttatccaca actgcgaggg cgactattcg
      541 gcttaggtaa ggcgaatttt tcgagttata cacatatcca caggccttat tattattatt
      601 gttctaatag atatatgtta ggtagtaata ataaggtgag aagggaggca gtatgagggc
      661 tgtttgtagc acggagacgt tcggcaaaaa gttggcgctg gtttcgcgcg gggtctctgc
      721 gaggtcgacc atccagttgc ttggggggat acttctcgag ggtaggggag aggcactggg
      781 gttatcggcg acggacatgg agatctcgat ccagacgtcg tcgcaggctg aggtcgatgg
      841 agaagggcgg gtggtgatac cggcccgcat cttcaacgac atcgtacggt cgctgcctgg
      901 cgggagcttt tcgctggagc acgacgactc ggaggggacg gtcaggctcg tggccgggga
      961 gaacgagtac cgcattcggg catacgcggc cgacgactat ccgcggcttc ccgtcttccc
     1021 ggaagagggc accttcagga tgtcagggga gtcgctcgtc gagaccgtgg agaaagtgtc
     1081 caggtcgtat tccagagacg agacaaggcc cgtattgacg gggatcctga tcagcttcga
     1141 ggaatcgcgc gtaaggatgg tcacgaccga ctcctaccgc ctcagcatca aggagactga
     1201 gctcgcgacc acctctttcg aggggtcgag ggaggcgatc ataccggcga gggcgatgca
     1261 ggaggtctcc aggatattct ccagctccga tgaggatgac gtcgaggtgg cgctctccga
     1321 gaaccaggcg ttgttcagga tcggcgacgt cgcgttgggc accaggctca tagacggcaa
     1381 ctttcccgag tacaggcgac tcttgccagg cggattcgag cgtgagatct ccgtttccag
     1441 ggaggagctg gtcggcaccc tgagacgggt gaacctcttc gccgcccgcc agacgccgcc
     1501 ggtccccgtg agcctctcgt tctccgaggg ttcagtggag gtgatcgtgc gaaacggcga
     1561 ggtgggagac gcacacgaac ggctgtccgc ctccagcgaa gacgagttcc tgatctcctt
     1621 caacccaggc tacctgctcg acggcgtctc cgcgatagat tcggagaacg tggtcttcaa
     1681 gctcaacgaa ccgctcaagc cagggctcat cgtgcctgag gagaacggtg aagagccaga
     1741 cttcctgtac ctgatcatgc cgatgcgcga tccgtcacgc ggctgaccct tgcggatccc
     1801 acctggcatg acgctgggcc aggctataaa ggcgtccggc ctcgtcggga cgggcggcga
     1861 ggcgaaagtc ctgatccagg ccggagaggt cagcgtgaac ggtgaggtcg agacgcgccg
     1921 cggtcgcagg ctcgaagagg gtgacgtggt cgaggtgggg gacgagcggt tggagatcgg
     1981 ttgagcgctc atctgcgggc cctacgcctg gtcaacttcc gcaactacgt cgatgccacg
     2041 gccctgctcg cccccggact gaacgtggtc gtgggggaca acgcccaggg gaagacgaac
     2101 ctgctcgagg ccatcgcctt caacgtcact ggctcctcgc cccgcacccc caaggagaac
     2161 gaggtcgtcc ggtggggcgc agacttcgcc cgcaccgagg cccgcatagc actcgacggc
     2221 tacacgggtg aacgcggcgt ggccatcggc tacgctccag ggcagaagaa gcgcatcacc
     2281 gtcgacgggg tcccggcttc ctccctcacc aactacgccg ccggcggcgc tggggcccgc
     2341 gtcgtcacgt tcttccccga cgacatcagg gtcgtgaagg gcgcgcctgc ggaccgcagg
     2401 gagtttctgg acgccctgct ctcctcgcta cgccccgctt acgcccgcgc cgcggtcgag
     2461 tacgccaggg cagtccagca gcgcaaccag ctcctgagac gcatcaggga tgggttctcc
     2521 tccgagagga cgctcgccac gtgggacaag aaggtcgtcg aactcggaaa aaccgtcctc
     2581 gccgggcgcg aggcggcgtt gccggttctc gaggaacatt tcatagacgc tctaagggcc
     2641 ctgtatgggc ccgagaaggc cgcgatacgc tacgggcata gttctaccct cgaagagtac
     2701 tccgaggcgc tcagagaggc gcacagcgcc gatgtcgaac gtgggaccac ttcggtcgga
     2761 ccgcacaggg acgacttcga ggtctctcta ggcggggtaa acctgaccac cttcgggtcc
     2821 caggggcagc agcggatcgc gacgctggcg ctgaagttcg cgtcgaggga ctacgtgcgg
     2881 ggggcagtgg gggaggaccc gatcctgctc ttcgacgatg tgatgagcga gctcgacgag
     2941 cgacggaggg agtatctggc cgggtatttt ctggagagca cccaggccgt gatctcgacg
     3001 accaacctcg aatatttcga cgaagagatc ttgagaagaa cccgtatcat acgcatatct
     3061 ggtggttcca tcctggagac cgcaacggat ggtgccagac gttagcgcga ccctgcgaaa
     3121 ccggcttaaa taagccgcaa atgggccgaa gtgctgtttg gaaacggcgg cgaatgtgtt
     3181 acaatcatcg ggtgcagagt tacgatgaaa agaacatctc cggcgccttc gccggactga
     3241 ccaagaggag ggtccggttc tttggcggct aagcccacga tcaagaatgc caagagcgct
     3301 tacgatgcaa gtaccatcca ggtcctcgaa ggcctcgaag ccgtcaagcg gcgccctggt
     3361 atgtacatcg gctcgacggg gcccaggggt cttcaccacc tcgtccggga gatcatcgac
     3421 aactccatag acgaggccct ggccgggtac tgttcggaga tactcgtaac gatccaccct
     3481 gacaactccg tcacggtcac cgacgacggg cgcggtatgc ctgtgggcaa gatggcgaag
     3541 tacggcaagc cggcggccga ggtcatcatg acgacattgc acgccggtgg gaagttcggc
     3601 ggggaaggtt acaaggtctc cggcggattg cacggcgtcg gcgcctccgt ggtgaatgcg
     3661 ctctccgagt ggctcaagtt ggagatcaaa gtcgagggac acgtgtggag ccagcgctac
     3721 gagcgcgggt ttccgaaggg cgctctgaca aaggaccgta agctcaagaa gggcgagggg
     3781 acgggcacca ccgtaagctt cctgcccgac cccgaggtct ttaccgagac ccaggacttc
     3841 tctttcgata ccttgagccg ctggttccgc gagtgggctt tcttgaacaa gggactcaag
     3901 atccggctcg cagaccagcg ggaagaagcc agggacgtca catatcagta cgagggcggc
     3961 ataagggact tcgtggagca catcaacgag gcgaaagacc ccgtccacaa aaccatcttc
     4021 tacctggagc gtgaggagga ggtaggggac gtggaagtcg cgatgcagtg gaacagcggc
     4081 ttccaggact ctgtcttcac gttcgccaac aacatcaaca ctcacgaagg cggcgcgcac
     4141 ctctccgggt tcaggtcggc gctgtcacgg acgatcaacg cctacgccag gcagaaaggt
     4201 cttctcaagg agaaagagga gagccttacc ggcgacgaca tcagggaagg tttggccgcg
     4261 gtcatctcgg tcaagttgcg ggagccccag ttcgaggggc agaccaagac caagctcggg
     4321 aacacggagg tcaagggtct cgtagagagc gtgaccaacc gttatcttgc cgagttcctg
     4381 gaggagcggc cgggtgaagc caaagcgatc gtcaacaagg ctttgcaggc ggcgagggcg
     4441 cggctcgctg ccaggacggt gagggagaag atccgcaagg gctacctgga gagctccacg
     4501 ctgccaggca agctcgccga ttgctcatcc aaggaccctg cgaggagcga gctgtacatc
     4561 gtcgaaggca actcggccgg cggatcggcc aaaggcggaa gggacagaaa cttccaggcc
     4621 atactgccgt tgcgcggcaa gatcctcaac gtagagaaag ccaacatcaa caagatcctc
     4681 tccaacgccg aaatacaggc cctgataagc gctatagggg ccggcgttgg ggagagcttc
     4741 gatctcgagc aagcgcggta caacaagatc gtcatcatgg ccgacgccga cgtggacgga
     4801 agtcatattc gaacgttggt gctgacgttt ttgtatcgca acatgcgaga tttgatcgag
     4861 gccggctacg tctacatcgc tcttcctccg ctctacaaga tcacgcacaa tcgccgcgag
     4921 cactacgcca agaacgacgc cgagctgaga gagacgatgg accgtatgag ggccaacgga
     4981 aacgccagta tctcccgctt caagggactc tcggagatga acccgcagca gctctgggag
     5041 acgacgatgg acccgcagaa caggacgatg ctccaggtca ctgtcgagtc ggcggcggtg
     5101 gctgatgagc tgttcaccgc gctgatgggc gacaaggtcg agccgcgcaa acagtttatc
     5161 gaggagaacg ccagagaggt gaaaaacctg gatgcttgat actttctcgg gcaacgtaca
     5221 gccccactcc atagaagatg agctaaagga ctctttcctt tcctacgcca tgagcgtgat
     5281 agtctcgcgc gccttgcccg acgcgagaga cggcttgaag cccgtccacc gccgcgttct
     5341 ctacgctatg caagatctcg gcctgcagcc caacaggcct taccgcaaga gcgcgacggt
     5401 ggttggagac gtcatgggca agtaccaccc gcacggggac caggcgatat acgacgcgct
     5461 ggcgcgcatg gcccaggact tcgcgctcag atacccgctc gtagacggcc aggggaactt
     5521 cgggagcgtg gacggcgatc caccgggcgc gatgaggtac acagaggcca ggctcacccg
     5581 tatggcgacc gagatgctga gggacataaa cgccgacacc gtggacttcg tcccgaactt
     5641 cgacgagagc ctgaaacagc ccgaggtctt gccggcgcgg ttcccgaacc tgctcgtcaa
     5701 cggctccgat ggcatcgcgg tcggcatggc caccaagatc ccaccccaca acctcggcga
     5761 ggtcatagac gctacgatgg ccctgatcga cgacccagat ctcgacgacg aggagatcgc
     5821 caaatatatc aaggggccag acttcccgac ggggggtctt atcgtcgggt tgcgcggaat
     5881 ccgcgacgcc atcgcgaccg ggcgcgggtc cgtacgggtc caggccaagg cccacaccga
     5941 gcagatcaag ggcaatagga cccagatcgt ggtcacggag atcccttacc aggtcaacaa
     6001 gagctacctg ctccagaaga tagccgagct ggtcaaggac cgcaagctgc aggacatctc
     6061 cgacctgcgt gacgagtcgg accggaacgg gatgcgcata gtgatcgagc tgaagcgcga
     6121 ggccgtgccc aaagtcgtcc tcaacaacct ctacaagcac acccagatgc agcagagctt
     6181 cggggtgaac ctggttgcgc tcgtcgacgg tgtgccgagg acgctctcct acaggcaggc
     6241 cctgggacac tatatcgccc accagttcga ggtggtaacg cgcaggacgc gctacgagct
     6301 cgggcgggcc agggcgaggg cccacatcct agaaggactg ctcatcgcac ttggggatct
     6361 cgacgaggtc gttgcgacca tccgccgctc gcgcagcgtg gagacggcgc gcaagaacct
     6421 gatgaagggg ttcgggctct ccgagcgcca ggcgcaggcc atcctcgacc tcaggctgca
     6481 gaggcttacc gcgatggagc gtcagaaggt ccagcaggag cacagggatc tgcgcgagaa
     6541 gatcgagtac ctggagggca tcctggccga tgagtacaag gtctacggga tcgtcaaggc
     6601 ggagctcggc gaggtcaggg ctgcctacgc ggacgatcgc agaacggcga tcacggccga
     6661 ggagggcgtc gacttcgaga tagaggacct catagccgag gaggagatgg tgatctccat
     6721 ctccaacggg ggttacctga agagcgtccc cgtcaactca ttccgcaagc aaggccgagg
     6781 tggggtcggc gtcgccggga tggagctcaa agagggcgac tacatagacc acctctttat
     6841 cacgacgacg caccactaca tgctcttctt taccaacaag ggcaaggtct acaggctcaa
     6901 ggtccaccag ctacctcgta tgggccgcgc ggcgaagggc aggcacatcg ccaacatgct
     6961 ccccctcgcg cagggcgagc acatcgcttc agtcctcgcc accaaagagt tcgaggaggc
     7021 cgagtacctg ctcttcgcga ccaggagagg tatcgtcaag aagacgaggt tcctggagta
     7081 caacacgccc ttgaagaacg atgggatcat cgcgatcaac ctcgctggcg acgacgagct
     7141 gatctccgtc cgttacgcct cagagggcga cgaagtcgtc atcgtgagcc gcaatggcaa
     7201 gggcatccgc ttcaagcaga ccgacgcacg tccgatggga cgtgctacag ccggggtcaa
     7261 gagcatcacg ctcggtaacg acgacgtggt gctctctatg gacgtgatct ccgacgacgc
     7321 tgcggacctt ttcatactca ccgagcgcgg tttcggcaag cgcacagccc tctcgcagta
     7381 cagggcccag ggacgcggcg gccagggcgt gatcgcgatg aaaaccgacg gtgaacgcgg
     7441 caaactcgcc ggtgtgcggg tcgtgcgccc tggactgcac gagctcatga tcgtctccaa
     7501 cttcgggacg accatccgca tagatgcgga ctcggtctcg cgacagggcc gcgcggcgca
     7561 gggtgtcagg gtgatgaacc tgaggagcgg cgactcggtg agcgccatag ccaaagtcat
     7621 ctcctcccgc tccgccgacg ccgacggcgc cacggacgag ctgacgctgg aggaactctc
     7681 tggggatggg cagacgggca cctctaccgc agggcttccc gaacccgagg ggattggggc
     7741 ggtgccaggt gaatagcgag tgcgaaggcg tcgtcgagtg cacggccctg gtcctcgccg
     7801 agagttgtac ggagtacgaa ggtaggctga cgctcttcgg ggtgctcgat gagatccgcc
     7861 ccaacggcgc cagcgggaca tccttcgtcg tctacaccaa gttcgagggc tgcgaacacg
     7921 tctcggacag ggagcgccgg gcgcgcatca tcgtcaccga cgaggacgga gaggtcttga
     7981 tggagtcggc ggagtattcc gtctacctcg acggatcaga agaacccgtc acgaaagtag
     8041 ccgccttcga cttacctgaa ggcttcgaga ccggggagca actactctgg atcgaggcgg
     8101 tcctcgacga ggagacgctc tcccagaccg tggtcaggat ccacgatcgc tacagtgtat
     8161 aagagaccaa cagaggcccg gatctccggg cctctcgtct acgttaggaa acctattgat
     8221 gcctgaagat ctggcagtat ttatcgactg ggagaacatc tacatctcca ctgtcacaga
     8281 gtacaaatcc aggccgaacg tctcggctat actggagaag gcccgcgagt acgggcgcat
     8341 cgtcgcggct acggcttacg ccgactggac cgacggagat ttccgccagg ccccgccgac
     8401 cctctattcg aacggcatct ctccgcgcta catatcggcc aggtacttcc ccggcggcaa
     8461 gtcccagaag cgccgtacca actccataga cgtgatgctc gcggtagagt gctcggactt
     8521 cttgcacaac cacccgcagg tggacaccta cgtcctcgtc acgggcgatg gggacttcat
     8581 cccgctcgtc aacctgctca ggagcaaggg gaagaacgtg gtcgtaatcg gggtctccga
     8641 ggcgacctcg taccacctga tcgaatcggc agaccacttc gtctcctacg cctctcttct
     8701 cgaagaagac cgcgccgccc gcacgcagga cagggaaccc aagaaggagg ccgcggaccc
     8761 ctacaaggag ctggtcaggg ccgtccagac attgaagaag ggtggcaaga cacgggtcct
     8821 cgggcaggtg aagcagcaga tgatcgtgca gctcggctca ttcgacgagc ggaacgcagg
     8881 cttcaagaag ttcaaggatt tcgtggtcga agcagagcgc cgcggcctgg taaacaccgt
     8941 ggaccaggac ctcgagctcc aggtctacct gcccgacgag caggttcccg acctgcaaga
     9001 gaccgacctc gcaaccgagg agcccagctc acagagccaa tccgcaagcg ggaacagggg
     9061 caacggcagg cccgcgcgag aagtgcgcca gcaagagccg gaacccggct tcgacctcct
     9121 cgaagacctc acgccctacg agctcaacac ggtctgcacg agcatcgcct ccctcgaaca
     9181 gcctgccacc ttcgtggaga tcttctccag catccgcgat ctggacgagc gcggcgaact
     9241 ggagctctcg cgggaggaga tcggggacgt cataaccgcc atgctcgacg cggattacct
     9301 gggcaaggtg cgccagaggc cttacaaaca ggcgcaggcc gacatgactt tctacgccct
     9361 gaacgcggaa aagcaagagg tcaagagcgc actgaacagc ggctgatatg ctgctctagt
     9421 cagccagctt gtagacggtg agggcggcga ggcagagggc cgccgctatc tggaccagag
     9481 acaacccaac tatcccggct acctgtatcg ccgagacgct gctgaacagg acctgcacgg
     9541 cgactattgc gccgaaaccg atggcgaacc acagtatcgc cttggtcctg tctccttcgc
     9601 tccatgtagc cgtcccgagc agcgcgggga cgtagaacgc caggatcaaa ggtatcaggc
     9661 gcaggatacc aacacctatg tcgagcatcg tctccatgat aaaagtctac accataggtt
     9721 ccgggatact ggaaaattgt gcacgccgta tgtacgctag tacgctaata cgattacaat
     9781 taaatagagg tgatggcgtg tgggaataaa tcagagggtt tcgctcagga agttcataga
     9841 cgacatccgc cgttgggttg aggaggaccg cagcgacgag tggatcgcaa gcgccttggg
     9901 gtcgagccct tcgtcggtgc agtcgttccg ctcgcgcaac gccatctacc gccgcggagc
     9961 cgcctcgacg ctgcacgacc cgcaggacta ctcctcctac gaaggggtgc tcgagccgga
    10021 gggtcccggc atctggttcg acc
//
LOCUS       DASTKM010000041         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_535807, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000041 DASTKM010000000
VERSION     DASTKM010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535807"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFQ10_01590"
     CDS             complement(<1..1175)
                     /locus_tag="VFQ10_01590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET9964061.1"
                     /translation="MGLGSFNKQSRQGLFRILSGGTLDLLVIGGGITGASVFRDAALR
                     GMSVALVEANDFASATSGRSSKLIHGGLRYLKNLGFRLAWESCHERNLHVRLNKRLVR
                     PMPFLVPLYRGQGEPRALMRLGMVLYEGLSGFHNYRLHRFMSREETLSLAPALPVDGL
                     TGGCLYYDAVVSDNRWTVETVKDGVRHGGVAVNHAPITGLLREAGKVIGGRIQDNVGG
                     EHYEVHARAVVNATGVFADQIRRMDDPDAARLVRLSKGTHLVFAEEDVPLSVTVVFSS
                     PLDKRSLFLAKHEGCFLYGTSDDWEESDPGAPVPAESDVDYLLATLDRFMPEAGLDGE
                     KVRFVYSGFRPLLFSGDGDLDSAAASREDHIEVSPSGLISVIGGKLTTARIMAIRVL"
     gene            complement(1183..>2297)
                     /locus_tag="VFQ10_01595"
     CDS             complement(1183..>2297)
                     /locus_tag="VFQ10_01595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9964062.1"
                     /translation="RIASRGKKVDRHENLADLAQAREMYLKSLEAFGRYRSFEATYRI
                     PLDDLSPGDTIGALVEALRPYLLADQEETLASGEPLGTSRRSGNTMRKTALDHRYLIR
                     YLLAKWFRGAWREPTFLFSSLGRLFLDEGYSAGVMRVIYDQDEKRYGLLDRVFLEYPL
                     HRAVYDRLQILTRKIEAELEARLQAGREVRIFTAPSGFAYDLFRPLETIAGRLPELMD
                     RVEIVATDLDPHEALAGELAQRAEKLGIRFTFLRGDMTDGEMRQRFEVKAPYDVALFV
                     GLSSWLPKPQILDHLRWIRKNLREDGLLVTDSFTPEAYALSGRYVGYKANYYEPEVYR
                     VLVDYCGFDGLGADVESGRDRINHVLIASPRVRRDE"
BASE COUNT          456 a          720 c          698 g          423 t
ORIGIN      
        1 tccaggaccc gaatcgccat gatccgcgcc gtcgtcaact tgcctcctat tacgctgatc
       61 aggcccgacg gagagacctc gatatgatct tcgcgactcg ctgcggcgga gtctagatcg
      121 ccgtccccag aaaatagaag cgggcgaaag ccactgtaca cgaaccgcac cttttcgcca
      181 tccaaccccg cttcgggcat gaaacgatcc agggttgcca gcaggtaatc gacatctgac
      241 tccgcgggta ctggcgcgcc aggatcggac tcttcccagt cgtccgaggt tccgtacagg
      301 aagcagccct cgtgcttggc cagaaagagc gagcgcttgt cgagcggcga ggagaaaacg
      361 acggtgaccg agagcggcac gtcttcctcg gcgaacacca ggtgggtccc tttggagagt
      421 cggaccaaac gcgcagcgtc ggggtcatcc atccgtcgga tctggtccgc gaagacacct
      481 gtggcgttga cgaccgccct ggcgtggacc tcgtagtgct ccccaccaac attatcttgg
      541 atgcgccctc caatgacctt gccagcctct ctgagtaacc ctgtgattgg agcatgattg
      601 accgccaccc cgccgtggcg cacgccgtct ttcacggtct cgacggtcca gcggttgtcg
      661 ctcacgacgg cgtcgtaata gaggcatccg cccgttagcc catctacagg cagggcaggg
      721 gctagagaga gcgtctcctc acgactcatg aatcggtgaa ggcggtagtt gtggaagccg
      781 gagagcccct cgtataacac catacccaga cgcatcaacg cccgtggctc tccctgcccc
      841 cggtagagtg gcacgagaaa gggcatggga cgcaccaggc gcttattgag ccgaacgtgg
      901 aggttacgtt cgtggcagga ctcccacgca aggcggaagc ccagattctt gaggtaccgg
      961 aggccgccgt gtatgagctt ggaactgcgc ccggaggtag ccgaggcaaa gtcattggcc
     1021 tcgacgaggg caacgctcat cccccgaagc gcagcgtcgc gaaagacgga cgccccggtg
     1081 atgccccctc cgatgaccag gagatccagc gtcccgcctg agagtatacg gaacaggccc
     1141 tgccgcgact gtttgttgaa cgagcccagt cccatcctgg ggttactcgt cccgccgcac
     1201 gcgtggtgag gcgatgagca catggttgat cctgtcccga ccggattcca cgtcagcgcc
     1261 cagtccatcg aacccgcagt agtcgaccag cacgcgatac acctcgggct cgtagtagtt
     1321 ggctttgtag cccacgtacc gccctgagag cgcgtaggct tccggggtga aggaatcggt
     1381 caccagaagg ccatcctcac gcaggttctt ccggatccag cgaaggtggt cgagtatctg
     1441 gggcttgggc agccagctcg acagaccgac gaacagggct acgtcgtagg gggccttcac
     1501 ttcgaacctc tggcgcatct cgccgtcggt catgtctccg cgcagaaacg tgaaacgtat
     1561 tccgagtttt tctgccctct gggcaagctc tccggccagg gcctcgtggg ggtccaggtc
     1621 ggttgcaaca atctccaccc ggtccatgag ttcgggcagg cgtccagcga tggtctccag
     1681 gggacgaaag agatcgtagg caaagccgct cggagcagtg aatatgcgaa cctcccgtcc
     1741 cgcctgcagg cgcgcctcca gttcggcttc gatcttcctc gtcaggatct gcagccgatc
     1801 atagaccgcc cggtggagcg ggtactccag aaagacgcgg tcaagcagac cgtaacgttt
     1861 ttcgtcctgg tcgtagatca cacgcatcac gccggcggag tacccttcat cgaggaagag
     1921 ccgtcctaga gaggagaaca ggaaggtcgg ctcccgccac gccccacgga accacttggc
     1981 cagcaggtaa cggatgaggt agcggtgatc cagcgccgtt ttgcgcatag tgttgccgct
     2041 gcgcctgctc gttcccaacg gctctccaga tgccaaagtc tcctcttgat cggcgaggag
     2101 gtacggtcgg agtgcttcta ccagcgcacc gatggtatct cctggagaga ggtcatccag
     2161 cggaatgcga taggtggcct cgaaagagcg gtaccgcccg aacgcttcga gagatttgag
     2221 gtacatctcg cgcgcctggg cgagatccgc cagattctcg tggcggtcga ccttctttcc
     2281 tcgcgatgcg atgcgcg
//
LOCUS       DASTKM010000042        10936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_359689, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000042 DASTKM010000000
VERSION     DASTKM010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359689"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFQ10_01600"
     CDS             <1..266
                     /locus_tag="VFQ10_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET9964063.1"
                     /translation="FEPVHGSAPDIAGQGIANPIGQIWSGAMMLDHLGHEDAGAGVVS
                     AIEQVLGETEVRTPDLNGSATTSEVTDAILGALEKMRSEDARQ"
     gene            302..445
                     /locus_tag="VFQ10_01605"
     CDS             302..445
                     /locus_tag="VFQ10_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964064.1"
                     /translation="MDKLIGRVVFDPESGVITQVLGRSYRDYAETRTHVRRELSEYME
                     RST"
     gene            442..2118
                     /locus_tag="VFQ10_01610"
     CDS             442..2118
                     /locus_tag="VFQ10_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET9964065.1"
                     /translation="MKAALYCRVSTEEQASSGFSLRQQLEALRRYCKDHDIEVVGEFQ
                     DTSSGANLDRPGLDALRDRISLGGVELVLCQDRDRISREPAHVYILREELLEYGTTLR
                     SLNDRGDDSPEGALHDGILDQLAKFERAKTAERTRRGRMRKAQEGKIVGTGKAPYGFY
                     YADDHYHVDPDRMPFVHEIFEMVADGHTIYEVAQHLRRNGTPSPRGADGRWGRTTIRN
                     IILSDTYLGTFWWGKEKRTTPTVSVVENGVRTYKKKVKRKDRPREEWTAIPVPDSGIP
                     PETIYRARSMVENNTWNVSPNPARTWELSGGIGLCGQCGSRLKTYSTSNAYKTKYFYY
                     LCSNRISSRENGTCPNTKHYPAETLELLVKDTLVDAFREESWIDFVNDTCDQRLRDLR
                     KMHRSNPLKARERLSKRIDALGRKETRLKDLYVDGDISKEEYGERKIVLEEGIAQITD
                     ELSKLDDLDGELHRIEHLRVALLSVESPLSGHYCFTETIADLDDDMMDNGLSYGSRET
                     AARRRQEFYRQAGLRVKVGEELEISLGVDGISVSADESASGSTRSPTGTP"
     gene            complement(2063..2230)
                     /locus_tag="VFQ10_01615"
     CDS             complement(2063..2230)
                     /locus_tag="VFQ10_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET9964066.1"
                     /translation="MVEIGVIGGDGIGPEVVRAGMRVLEAAAKDDPSFELGFTEFPWG
                     CEYYLKHFHLH"
     gene            2403..2738
                     /locus_tag="VFQ10_01620"
     CDS             2403..2738
                     /locus_tag="VFQ10_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964067.1"
                     /translation="MDERDARRNREASERLDRLTREGYKTAVDQAFEAQKSGMRLSRR
                     FFENWVEALDDQAELNRRALGSLQQLVREQSEVFRVLSRESVDAYDGFLNSLDAYEED
                     ISNLDKRED"
     gene            2834..4225
                     /locus_tag="VFQ10_01625"
     CDS             2834..4225
                     /locus_tag="VFQ10_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET9964068.1"
                     /translation="MREEELPRSLSEGPRSGGFDGVNPLATDVLRRNGLKSWTKPAPS
                     LYPHQWSWDSAFIALGLAHVDNRRATDELETLFTGQWSTGKVPHIVFNPEAPPKSYFP
                     DAERWNSSALSEDAPSGPRTSGLCQPPVHAIAVRRIWQTARGKDEERVERARSFLSGN
                     YRRLFSWHRYLATARDPEGSGLVTIYHPWESGTDNSPRWDAALERISVGEVPSYTRYD
                     LEHVTDPSHRPTDLEYDRFLWLLELLKRVRYDEADIYERHPFLIKDVLFSAILVAANE
                     ALLEISEVVEAPREDRRLIEGWIERGREGLEAQWDPELGLCLDQDVLADEPLRVRTVA
                     GFAPLISGGLDRSRLSDLLETLDSQAFTGNPRLSYPLPPSTSPEEPGFHPRSYWRGPV
                     WPVANWLLWWSLLRAGESEKAQRIRRETLEELSEGGFAEYFEPFTGEPLGSGEQSWTA
                     AVALDMLATGGAD"
     gene            4333..4971
                     /locus_tag="VFQ10_01630"
     CDS             4333..4971
                     /locus_tag="VFQ10_01630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9964069.1"
                     /translation="MIERLVRDGSAVARSDGSLHSIFPVAVYAAEGEALREWVLREGA
                     TKTIEIGLGYGISALHVCEGLLVNADPTARHVALDPYQATRFADCGLQFLEEAGVDEM
                     VELHAEESQIALPRFLGEARRFHLAFVDGNHRFDGIFVDLVFLGRLVRAGGIVFVDDY
                     QLPAVARAVSFCVTNLDWTLEEVSATDELHRWAVLRTSAVVDMRPFDYYVDF"
     gene            5085..5777
                     /locus_tag="VFQ10_01635"
     CDS             5085..5777
                     /locus_tag="VFQ10_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964070.1"
                     /translation="MGEVDTLRGVWLAALAIFRRYPLATLVPAAILGAIGEVPAYLID
                     GRPVLDQALTLVTTYVAYYLYLAYAEGIVRRAQRGVQRSGLQSMLDDLMEAAPFVPSV
                     LVAALISLSMTTIATGLLVIPGVWLYTRWSLTTPVIREESIGPLAATRRSNQLVSGRF
                     WFVFMTATVAYYLEGVVIHESALVAGSVTGSHTWGAWTGGSIAATLVVPLAAFATSLA
                     HSSVASRALPLA"
     gene            5958..6464
                     /locus_tag="VFQ10_01640"
     CDS             5958..6464
                     /locus_tag="VFQ10_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HET9964071.1"
                     /translation="MLGKAVEEALNEQIKNELYSAYQYLSMAAYCESENLPGFAQWMR
                     AQSREETGHAIKFYDFILERNGRVVLRAIDGPVVEFGSPLEVFEQALEHEKKVTAMIN
                     DLYGLAVRENDYASQTFLQWFVTEQVEEEKNAGDVVETLKMVGDKSEALFLLDRELGQ
                     RRVEQQAG"
     gene            6587..7282
                     /locus_tag="VFQ10_01645"
     CDS             6587..7282
                     /locus_tag="VFQ10_01645"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HET9964072.1"
                     /translation="MNERQEYRGEDRGRKELADRLREALSRPLDEWREVYEGFSPVDL
                     ESGERRPRVPPAGADTRRAAVLVPIIVEGDDARVVYTVRKDDLADHAGQISFPGGSIE
                     ARDTSLLETALREAEEEIDLRPELVEVVGELEEMYIPPSNFRVSPFVGLLPPEAEMVL
                     APDEVEEIFTVPLKTLTAHETFRKVLWRREGRDYKVPVFAIEGPVPRNIWGATAAMTA
                     ALLARLGWREYRH"
     gene            7292..7687
                     /locus_tag="VFQ10_01650"
     CDS             7292..7687
                     /locus_tag="VFQ10_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964073.1"
                     /translation="MLRVVAMALFGLMFLAEVGDLYGLVLTLADPMPAAERFGITAGA
                     EVLRNTVLLILALVVCFGALLALVGLLARRPGLFHTSALVCAIGYLVYGLLQVVGGAL
                     QLGSAIVVVAGLIYVVLGGLAYAMYRSVY"
     gene            7839..8231
                     /locus_tag="VFQ10_01655"
     CDS             7839..8231
                     /locus_tag="VFQ10_01655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9964074.1"
                     /translation="MATETERDEGVRRVYKDHNLHVLWGVTLMAVLGTSSVTPAFPTI
                     VREFGISSGQVGLLITFFTLPGIVMTPLLGILSDRYVRKKILVPALLLFGLAGGASAP
                     SPATSMCSSPSAFSRVWGQPPSALSTSP"
     gene            8162..9025
                     /locus_tag="VFQ10_01660"
     CDS             8162..9025
                     /locus_tag="VFQ10_01660"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9964075.1"
                     /translation="MLLALRFFQGMGAAALGTLNVTVIGDIYDGRERSSALGYNSSVL
                     SVGTASYPAIGGLLATFGWFYPFALPFAAIPIAIVVMFSLHNPEPRNDDRLRDYFGSV
                     WENLYDREVLGLICASLLTFIVLFGPQISYLPILMNARFDAPSYVIGAVLSGASLTTA
                     LTSTQLGRLTGRFSEKGLVKTAFVLYAIALIAVALAPSLPLLVLPAVLFGVAQGINLP
                     NVFSLLNAHAPTENRGAFMAANGMSLRAGQTIGPLFMASAAGTLGLTGAYLAAAGLAL
                     FAFLLVLTLVR"
     gene            9495..10637
                     /locus_tag="VFQ10_01665"
     CDS             9495..10637
                     /locus_tag="VFQ10_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9964076.1"
                     /translation="MEGEVLRLCSLEEEGLVCFSARSGWDLWLGAQGLRVGDEVLVSA
                     VTHPDMVRIISEHGLRAVPVDIDPETLAPLPCMLQAALTPRTRVVLVAHLFGGRMDLG
                     VVAKFARERGLLLVEDCAQAFQGPERMGDPAADVSIYSFGTLKTSTALGGAVLRVRDR
                     RVLGRMCGIQASYPTQGRGGYLKKLLKVLGLVAVSRPRPYGLLARACARLGSDLDALV
                     NGVVRGFPPHERAATFLGRLRQRPSAPLLAMLSRRLRTFDGERLARRTSTGERFPRRL
                     RVVDLHPGQRSLQRTHWLFPVVVEDPEALVASLRRRGLDASQATSSIAVVETPAGCSS
                     PAEASLMMSGVVFLPMYPELPSQAFDIMAGLLNDCAAHVAAESVAL"
     gene            10637..>10936
                     /locus_tag="VFQ10_01670"
     CDS             10637..>10936
                     /locus_tag="VFQ10_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9964077.1"
                     /translation="MGGHRERTLEELGRSVFDVLVIGGGIVGGRVAFDAARLGLRVAL
                     VDAGDFGGATSGASARLVHGGLRYLGTGDFRLVRTALRERDVFASRVAPHLVRPLP"
BASE COUNT         1995 a         3246 c         3551 g         2144 t
ORIGIN      
        1 tgttcgagcc ggtccacggc tcggcgccgg acatagccgg acagggaatc gcgaacccca
       61 taggccagat ctggtccggt gcgatgatgc tagatcacct cgggcacgag gacgccggag
      121 cgggcgtggt ctcggccatc gagcaggtgc tcggcgagac ggaggtccgg accccagatc
      181 tgaatggctc ggccactacg agcgaggtga ccgacgccat cctcggcgct ctggagaaga
      241 tgcgcagtga ggacgcgagg cagtagccgg caccagctgc cgaacatgag aaaggagcga
      301 ggtggacaag cttatcggcc gggtggtgtt tgatcccgaa agcggcgtga taacgcaggt
      361 actcggcagg agctaccgcg actacgcaga gacgaggacc catgtcagga gggagttgag
      421 cgaatatatg gagaggtcca catgaaggct gccctatatt gccgggtatc cacagaggag
      481 caggcctcaa gcggtttttc tttacgacaa cagcttgagg ccttgcgtcg atattgcaag
      541 gaccacgaca tagaggtagt gggagagttt caagatacct cttcaggggc aaatctggat
      601 cgaccagggc tcgacgcctt aagggacaga atctctttag ggggcgtcga gcttgtccta
      661 tgtcaggaca gggacaggat cagtagagag ccagcacacg tctacattct gcgtgaggag
      721 cttctagaat acggcacaac cctgcgttcc ctcaacgacc ggggtgacga ctctcctgag
      781 ggagccttgc acgacgggat acttgatcag ctagcgaagt tcgagagggc gaagaccgca
      841 gagcggaccc gtagggggag gatgaggaag gcccaggagg gtaagatcgt tggcacgggt
      901 aaggccccct acggattcta ctatgccgac gaccactacc atgttgaccc agatcgtatg
      961 cccttcgtac acgagatctt cgagatggtc gccgacgggc acaccattta cgaggttgcc
     1021 cagcaccttc gtagaaatgg tactccctct cctagaggtg cggacgggag gtggggtagg
     1081 acaacgatcc ggaacatcat ccttagtgac acctacctcg ggaccttctg gtggggcaag
     1141 gagaagagaa ctaccccaac ggtctcggtg gtagagaacg gagtgcgcac ctacaagaag
     1201 aaggtcaagc ggaaggaccg tccccgagag gagtggacgg ccatccccgt gcctgattct
     1261 ggtatcccac ccgagaccat ctaccgtgcg cggtcgatgg tagagaataa cacctggaat
     1321 gtttccccta acccggctag aacctgggag ctttccggtg ggataggttt gtgcgggcag
     1381 tgtggttctc gtcttaagac ctactccacg tccaacgctt acaagaccaa gtacttctac
     1441 tacctatgct ccaatagaat ctccagccgg gaaaatggca cgtgtcccaa cacaaagcac
     1501 tacccagcag agactctgga attgctggtc aaggacaccc tcgtggacgc cttccgtgaa
     1561 gagtcctgga tagattttgt taacgacacg tgtgatcagc ggcttaggga ccttcgcaag
     1621 atgcaccgtt ccaatcccct gaaggccagg gaaagactga gtaagcggat agatgccctg
     1681 ggtaggaagg aaacccggct gaaagacctg tacgtcgatg gagacatcag caaggaggag
     1741 tacggggaga ggaagatcgt tcttgaggag ggtattgcgc agataaccga cgaactatcc
     1801 aagttggatg atctcgacgg cgagcttcac cggatagaac atctccgtgt tgccctgctc
     1861 tccgtggaga gccccctcag cggtcactac tgcttcacgg aaaccatagc tgatctggat
     1921 gacgacatga tggataacgg cctgtcctac ggctccaggg agacggcagc gaggcgcagg
     1981 caggagttct atcgccaggc gggattgagg gtgaaggtgg gggaggagct agagatcagc
     2041 ctaggcgtcg atgggatctc tgttagtgca gatgaaagtg cttcaggtag tactcgcagc
     2101 cccacgggaa ctccgtaaag ccgagttcga acgagggatc atccttcgcc gcggcctcga
     2161 gcacccgcat gccggcgcgc acgacctcgg gtcctatgcc gtcacctcct atgaccccga
     2221 tctcgaccac ggctctatgc tagcaaccgg ctgaggcagc tatcaagtac gtgagggata
     2281 taatcctgcc ggcgtaggga aagctgggag aggcggtgcc acagggcagt agacgtcggc
     2341 cgctactctt tagatcctgc acagattagc gggtatcata ggggtgggaa aggaggtctg
     2401 tagtggacga gagagacgcg cggcgaaaca gggaagcttc ggagcggctg gacaggctca
     2461 cccgcgaagg gtacaagacg gctgttgacc aggctttcga ggcgcaaaag agcggcatga
     2521 ggctctccag gaggtttttc gagaactggg tcgaagccct ggatgaccag gctgagctca
     2581 accgaagggc cctcggtagc ctgcagcagc tggtgaggga acagagcgaa gtcttcagag
     2641 tgttgtccag ggagtccgtc gacgcctacg acggctttct aaattccctg gacgcctatg
     2701 aggaagacat ctcgaacctg gataagcgcg aagattgagt ggaggctctt cagggtaggc
     2761 ttgaaggcgg aggccggtcc ttgtatacat tgaccgggta gacttagtcg atgggagtta
     2821 ctaacaccgg ttcatgcgag aggaagagct tccaaggagc ctttcggagg ggccacggtc
     2881 cggcgggttc gacggcgtca acccactcgc gacggacgtt ctgcgccgca acggcctgaa
     2941 atcctggacc aaaccagccc cttcccttta tccgcaccag tggagttggg actccgcctt
     3001 catcgcgctc ggactagcac acgtggacaa ccgccgcgcc accgacgagc tggagaccct
     3061 cttcaccggc cagtggtcga cgggcaaggt accccatata gtcttcaacc ccgaggctcc
     3121 gcccaagagc tacttccccg acgcggaacg ctggaactcc tcggcgctct ctgaggatgc
     3181 gccctctggt ccgcgcacca gcgggctgtg tcagcctccc gtgcacgcca tagccgtgag
     3241 gcgcatctgg cagacggcgc ggggcaagga tgaagagcgc gtagagcgcg cccgctcctt
     3301 cctcagcggc aattaccggc gtcttttctc ctggcaccgc tacctggcaa cagcccgcga
     3361 ccctgaaggc tcggggcttg tgaccatcta ccatccctgg gagagcggca cggataactc
     3421 cccgcgctgg gacgccgccc tggagaggat ctccgtcggg gaggtacctt cctacacccg
     3481 ctacgacctc gagcacgtta cagatccctc gcaccgtcct acagatctgg aatacgaccg
     3541 ctttctgtgg cttctggagc tcctcaagcg ggtccgctac gacgaggccg acatctacga
     3601 gcgacaccct ttcctcatca aggatgtcct cttttcagcc atcctcgttg cggcgaacga
     3661 ggcgttgttg gagatctccg aggtcgtcga agcgccgcgg gaggacaggc ggctcataga
     3721 gggttggatc gaacggggcc gcgagggtct cgaagcgcag tgggatccag agctcgggct
     3781 ctgcctggac caggacgtac tcgcggacga gcccctgcga gtgcgcaccg tggcaggatt
     3841 cgcgcccctg atctccggcg gactagatcg ctcacgtctc agcgaccttc tcgagacgct
     3901 cgactcgcag gctttcaccg gcaaccccag gctgagctat ccactgccgc cttccacaag
     3961 ccccgaggag cctggcttcc acccccgcag ctactggcgc ggtcctgtct ggcctgtagc
     4021 caactggctc ctgtggtggt cgctgcttcg tgcgggagag agtgagaagg cccagaggat
     4081 tcgccgcgag accctggagg agctctcaga ggggggcttc gccgagtact tcgagccgtt
     4141 caccggtgaa ccgctcggct ccggtgagca atcctggacc gctgccgtcg ccctcgacat
     4201 gctcgccaca ggtggcgcag actgacgccc tgaggagact cagacaggcg cgtaagtaag
     4261 agaggcgggc gatagcgggt ggtttccaca aacgttccag atcgaggctc ggccctgcta
     4321 cgggtgcgcg gcgtgatcga gcgcctggtt cgggacggaa gcgccgtcgc gcgttcggac
     4381 ggctcgcttc atagcatctt cccggtggct gtgtacgccg ccgaaggcga ggcgctgcgg
     4441 gagtgggtct tacgtgaggg ggcgacgaag acgatcgaga tcggcctcgg ctacggcatc
     4501 tcggcgctcc acgtctgcga gggcttgctc gtgaacgcag atccgacggc gcggcacgtc
     4561 gccctcgacc cttaccaggc aacgaggttc gcggattgtg ggctgcagtt cctcgaggag
     4621 gctggggtcg atgagatggt ggaactccac gcggaggagt cgcagatcgc gcttccgcga
     4681 ttcctcggcg aagcccggcg ttttcacctg gcgttcgtgg acggcaacca ccgcttcgac
     4741 gggatcttcg tcgacctcgt cttcctagga cggctggtac gcgcgggcgg gatagtgttc
     4801 gtcgacgact accagctgcc tgcggtcgcg cgcgcggtgt cgttctgtgt aacgaacctg
     4861 gactggacgc tcgaggaagt ctcggccacg gacgaactcc accgatgggc tgtcctccgc
     4921 acttctgcgg tagtggacat gcgaccgttt gactactacg tcgacttctg atgccctagc
     4981 cccgcatgcc acgtgtagcg gtttgcgacc ggagcacggt ggctgggagg tgcgaatcgg
     5041 cgcgtttgcc atgtgccgag ggctgggcga gaatgcgcgg gcgcatgggc gaggtggaca
     5101 ccctcagggg cgtgtggctg gcggccctgg cgatcttcag gaggtatcct ctggcgacgc
     5161 tcgtgccggc cgccatactc ggtgctatcg gggaagtccc cgcctacctc atcgatggtc
     5221 ggcccgtgct ggaccaggct ctcaccctgg tgaccaccta cgtcgcttac tacctgtacc
     5281 tggcatacgc agaggggatc gtcaggaggg cacaacgggg agtccaacgc tccggcttgc
     5341 agagcatgct cgacgatctc atggaggccg cgcccttcgt cccgagcgtc ctagtggccg
     5401 cgttgatctc cctctccatg accaccattg cgaccggcct gcttgtgatc ccaggggtgt
     5461 ggctgtatac gcgctggtcg ctgaccacgc cggtgatccg tgaggaaagc atcgggcccc
     5521 tcgctgcaac acgacgcagc aaccaactcg tgagcggtcg cttctggttc gtgttcatga
     5581 cggcgaccgt agcctattat ctggaagggg tagtgatcca cgagagtgcg ctggtggccg
     5641 ggtcggtcac agggtcgcac acgtgggggg catggacggg aggttcgatc gcggcgacgc
     5701 tggtcgtgcc cctggcggcc ttcgccacct cgttggccca ctccagcgta gcgagtcggg
     5761 cgttgcccct tgcataaggc aacatgctac aggtgtcgga tatcatgacg accgggtcgc
     5821 ccttcccgac ggttccaacc atcagcacga cccagtagac gccgaccggc tagtctgcga
     5881 ttcttgcagg ctctccatcc ggggatcggt atagtaaggg cagcgattta gcgaaggacg
     5941 agaaggagga gatcaccttg cttggcaaag cggtcgagga agcgttgaac gaacagatca
     6001 agaacgagct gtattcggcg taccagtacc tctcgatggc ggcgtactgc gagtcggaga
     6061 accttcccgg cttcgcgcag tggatgcgcg cccaatctcg ggaggagacg gggcatgcga
     6121 ttaagttcta cgacttcatc ctcgagcgca acggtcgggt cgttctgcgg gccatagacg
     6181 ggcccgtggt cgagttcggc tcgccgctgg aggtcttcga gcaagccctg gagcacgaga
     6241 agaaggtcac tgcgatgatc aacgacctct acggactggc agttagggag aacgactacg
     6301 cgagccagac tttcctgcag tggttcgtta cggagcaggt cgaggaggag aagaacgcgg
     6361 gggacgtcgt cgagaccctg aagatggtcg gagacaagtc cgaggcgctc ttcctgctcg
     6421 atagggagtt aggccagcgt cgggtggagc aacaggccgg ttgaggaacc agatctcgtt
     6481 gcggtgacct gtccgcagcc tgttggtgga agatcacatt ggctcaacag atgactcctc
     6541 gccaccgtgc gtaccgaggg catgttggta cgctatgatc tcgatgatga acgaacgtca
     6601 ggagtacagg ggcgaggatc gcggccggaa agaactcgcg gatcggctgc gtgaggctct
     6661 ctcccgcccg ttggacgagt ggcgcgaggt ctacgagggc ttctcgcctg tggatctcga
     6721 gtctggtgaa agacgtccgc gggtgccgcc ggctggcgct gacacgcggc gcgcggccgt
     6781 gcttgttccg atcatcgtag aaggcgatga cgcgcgggtc gtctacacgg tgaggaagga
     6841 tgatctcgcc gaccatgccg gccaaatctc ctttcccggc ggcagcatag aggcccgtga
     6901 cacttcgctc ctggagaccg ccctacgcga ggccgaggag gagatcgacc tcagacccga
     6961 actggtagaa gtggtagggg agttggagga gatgtacatc ccgccctcga acttccgcgt
     7021 cagccccttc gtcgggctcc tgccgcctga ggccgagatg gtgctggccc ctgacgaggt
     7081 ggaggagatt ttcacggtcc ctctgaagac tctgaccgcc catgagacgt tccgcaaggt
     7141 gctctggcgc cgcgagggcc gcgactacaa ggtgcctgtg ttcgctatcg aggggcctgt
     7201 cccacgaaac atctggggcg cgacggcggc gatgaccgca gccctgttgg cacgcctggg
     7261 ctggcgggag tacaggcact agggggacgg tgtgctacgc gtggtcgcca tggcgctctt
     7321 cggcctcatg ttcctggccg aggtcgggga cctctacggg ctcgtcctca cgctcgctga
     7381 tcccatgcct gcggcagagc ggttcggtat caccgccggg gccgaagtcc tgcgcaacac
     7441 cgtactcctg atccttgccc tcgtcgtttg ctttggcgcg ctactcgccc tggtcggatt
     7501 actcgcccgg aggccggggc tctttcacac gagcgccctg gtgtgcgcga tcggatacct
     7561 cgtgtacggc ctcttgcagg tggttggcgg cgcgctgcaa ctcggctcgg ccatagtggt
     7621 cgttgccggt ttgatctacg tggtgctcgg cggtctcgct tatgcgatgt accggtccgt
     7681 gtattagaac acttcagcgt gtcagcacgc gccctgtcgg gagctttcag tacttcagct
     7741 tgtcgcatgc tggcccttgc tgacaagcgg aggccgaagc gcgctgggag tgtgcctgat
     7801 cccacctccg aacacacccc ctgatagatt ctcctatcgt ggctacggag accgagagag
     7861 acgagggcgt tcgccgggtc tataaggacc acaacctcca cgttctgtgg ggcgtaactt
     7921 tgatggcggt cctcgggacc tcgagcgtaa cgcctgcctt tcctaccatc gtacgggagt
     7981 tcggcatctc cagcggacag gttgggctcc tgatcacgtt cttcacactc cccggcatcg
     8041 tgatgacccc gcttctcggc attctctccg accgttacgt aaggaagaag atcttggtcc
     8101 ccgccctcct ccttttcggc cttgccggcg gcgcctctgc gccttcgccc gcgacttcga
     8161 tgtgctcctc gccctccgct ttttccaggg tatgggggca gccgccctcg gcactctcaa
     8221 cgtcaccgtg atcggcgaca tctacgacgg ccgcgagcgc tcgagcgccc tcggatacaa
     8281 ctccagtgtc ctctctgtgg gaacggcgag ctacccggcc ataggcggtc tgctggcgac
     8341 cttcgggtgg ttctacccat tcgcccttcc cttcgctgca atacccatag ctatcgtggt
     8401 catgttctcg ttgcacaacc cggagccgcg caacgatgat cggctgaggg actacttcgg
     8461 gagcgtgtgg gagaacctct acgataggga ggtcctcgga ctgatctgcg cttccctgct
     8521 tacgtttatc gtgctcttcg ggccgcagat atcgtacctt ccgatcctga tgaacgcgcg
     8581 cttcgatgcc ccttcctatg ttatcggggc cgtcctctcg ggcgcctccc ttacgaccgc
     8641 gctgacctcg acgcagctag gccgcctgac cggccgtttc tcggagaagg gcctcgtgaa
     8701 aacggctttc gtactctatg ccatagccct catcgcggtc gctcttgcac ctagcctgcc
     8761 ccttctcgtg ctccccgcag tcttgttcgg agtggcgcaa gggatcaacc tccccaacgt
     8821 cttctcgctc ctcaacgccc acgcccccac agagaaccgc ggggctttca tggccgcgaa
     8881 cggtatgtcc ctccgcgccg gccagaccat agggccactc tttatggcct ccgccgcagg
     8941 aacactcggc ctgacaggag cctacctcgc cgccgccggc ctcgccctct tcgcattctt
     9001 actcgtcctc accctcgtgc gatagagcat ttcagctgtt cagcacgctc aggcttcgcc
     9061 ttcgctcgtc agcacatcag ctttgttagg gagcggctgc gttggtcgtt ctctctgaat
     9121 cagatagggc ctgcatcgat taccacgtag cttggctcgg tgtgggagtc tcgctggcag
     9181 gtacttgctg aagtgctgac aagcccctga aaggcggcgt gctgacttgc tgatctgcac
     9241 gtgtattgga agctttgcgc ttccaataca cgctgtgtgg cgccaagggg tcttacagtg
     9301 taaacttacg tcgtaagata ctggttgcaa cgaaggggtg aggcgttgtg ggttcgagtg
     9361 ggtgggggag gttagggagg acggatggcg tgaggatgtg gcgtggcgta tatgcgcggc
     9421 acaggttgga catctcggtg gaggatatat gtttcggtat tctttcgtgt ggctggcagt
     9481 tgagaaggga ggagttggag ggtgaggttc tgcggctatg ctctttggag gaagaggggt
     9541 tggtttgttt ctcggcgcgg agtgggtggg acctgtggct tggcgcgcag ggtctacggg
     9601 ttggggatga ggttctcgtc tcggccgtta cgcaccctga catggtccgg atcataagcg
     9661 agcatggtct gcgcgccgta cccgtagaca tcgaccccga gacgctcgcg ccgctgccct
     9721 gcatgctcca ggcggccctc acgccgcgca cgcgggtcgt gctggtcgcg cacctgttcg
     9781 gtgggcgcat ggatctcggg gttgtagcga agtttgcgag ggagcgcggc ctgctcctcg
     9841 tagaggactg cgcccaggcc ttccaggggc cggagaggat gggagatccg gcggcagacg
     9901 tctccatcta cagtttcggc accctcaaga cctcgaccgc gcttggcggc gcggtcctgc
     9961 gggtacgcga cagaagggtg cttggtagga tgtgtgggat ccaggcctca tatcccactc
    10021 agggccgggg cgggtatctg aagaagctgc tcaaggtcct cggcctcgtc gccgtctcgc
    10081 ggccccgccc ctacgggttg ctcgcgcgag cctgcgcgcg attaggctct gacctcgacg
    10141 ctctggtcaa cggcgtggtc cgtgggtttc ccccgcatga acgtgctgcg acgttcttgg
    10201 ggcgtctgag gcagcgaccc tccgcgccgc tgctcgcgat gctctccagg aggttaagga
    10261 ccttcgatgg ggaacggctg gccaggagaa cctcgaccgg tgagcgattc ccgcgcagac
    10321 tgcgggtggt tgatctgcat cctggtcagc gatcattgca gaggacgcac tggctcttcc
    10381 ctgtggtcgt cgaagatcct gaggccctgg tcgcgagcct ccgccggcgc ggtctggacg
    10441 cgagccaggc taccagtagc atcgctgtcg tcgaaacgcc cgcagggtgt tcgtcgcccg
    10501 ccgaagcttc gctgatgatg tcaggcgtcg tgttcctgcc catgtatccg gagctccctt
    10561 cacaagcctt cgacatcatg gccgggctcc tcaacgactg cgccgctcac gtagcggccg
    10621 aaagcgtggc gctgtgatgg gtgggcatcg ggaacgcaca ctggaagaac tcggtcggag
    10681 cgtgttcgac gtactcgtga tcggtggcgg catagtcggg ggcagggttg ctttcgacgc
    10741 ggcgcgatta ggacttcggg tggcgctcgt ggacgccggg gactttggag gggcgacttc
    10801 aggagcctcc gcgcgcctgg tccacggtgg gcttcgctac cttggaacgg gtgatttccg
    10861 gctcgtgcgt acggccctaa gggagcgcga cgtcttcgcc tcccgcgtcg cgccgcatct
    10921 ggtcaggccg ctacct
//
LOCUS       DASTKM010000043         7643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1416843, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000043 DASTKM010000000
VERSION     DASTKM010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416843"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /gene="serA"
                     /locus_tag="VFQ10_01675"
     CDS             complement(<1..1046)
                     /gene="serA"
                     /locus_tag="VFQ10_01675"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET9964078.1"
                     /translation="MRVLVPEKLADPGIELLEQDFEVDVFLDLSPEELLQKIGDYDGL
                     IIRSATKVTADVIERAVNLKAIGRAGIGVDNIDIEAATKHGIIVANAPESNTVAAAEH
                     TLGLMLAVARRIPAADSSVRRGEWKRSALKGVEVSEKTIGLVGLGHVGAIVARGALGM
                     GMRVLAYDPYVSEDRMRSMNVGRAESADEVFERADYVSLHVPRTPQTLGLVNEEALTK
                     MKPTAYLINVARGGIVDETALYNALKEGAIAGAALDVFAEEPTTDSPIFTLPNVVVTP
                     HLGASTAEAQDRAGITAAEQVATALRGAVPVHAINAPVPVGEGAEFVAQFSGLCESLG
                     SLLYQLTDDTGDTL"
     gene            complement(1043..2200)
                     /locus_tag="VFQ10_01680"
     CDS             complement(1043..2200)
                     /locus_tag="VFQ10_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET9964079.1"
                     /translation="MMNKYRLMSPGPTPIPPEVSAAGALPIIHHRTPEFGEVFTRVNE
                     NLKRVFLTENDIFTYAASGTGAFEGAIQNLFSPGDKILVVNNGNFGGRWVKMSRAFGL
                     EITELEYDWGQKADNDEVAEALANDPEIKAAICVLSETSTGTVNDIEGFARAASDVVT
                     IVDAVSGLGACTLRTDEWGVDVVVAGSQKALMTPPGLGFVSVSERAMRMHEESLMPRY
                     YFDWTAARKAYEKDPAQTAWTPAVSLIIQLDVALQQLIDEGIENVFQRHVLLGRASRA
                     GIKGLGLELFGPDEDPNSAVTAAWVPEGIDGKQLVRMIFREHGIQVAGGQGEMAGRIF
                     RIGHCGYFDAFDIIATIAATELALESLDYPVELGKGVGAAQRVFSKSGVRA"
     gene            complement(2289..2486)
                     /locus_tag="VFQ10_01685"
     CDS             complement(2289..2486)
                     /locus_tag="VFQ10_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964080.1"
                     /translation="MTRLAVIGLGYRATAMIASMQAVDTGVSVRAVADPATESARRRP
                     ASNKWAAAGCRDRGEAESNTL"
     gene            complement(2662..4533)
                     /locus_tag="VFQ10_01690"
     CDS             complement(2662..4533)
                     /locus_tag="VFQ10_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HET9964081.1"
                     /translation="MATIEDMILLPKPRSVVARTGAYRLTGGSKIICQGDPGSLFPIA
                     HRLQRALWENQRIGWTLRAGDPGIYDTRGGTTMTLALEGGIPAQGYRLRIADDGIDLV
                     AGDAAGAFYGVMTLVQMLRQSEGALPAGEIEDSPDFPSRGVMLDISRSKVPTLQTLFD
                     LVDLFSGWKINHLELYTEHTFAYENHREVWAQASPMTGEDILRLDAYCRERFVELVPN
                     QNSFGHMHHWLERTRYNHLAECPDGFELTWQGQTRQMPPFSLNPTDRRSVEFMGELFS
                     ELLPHFSSEKFNVGCDETWDVGKGRSAQAVEEKGAGRVYLDYLLGIYDLVKHHRRTMH
                     YWGDIIIQHPDLVPELPKDSVVLEWGYEADHPFEEHGAEFARSGIPFFVCPGTSSWNT
                     IAGRTDICLANVRNATENGLRHGAIGLLNTDWGDNDHTQYLPVSYLGFAAGAALPWRH
                     ETNRDEDFIAALDLHAFHDEARVMGRLSHDLGNAHEKAGPVPHNSTVLFHLLTQAPGS
                     PLPDGVTLESLRVAGEHIGSIIEPLEGARMARVDAGIIRDEFANAARMMLHACERGTA
                     ILQGTIDAAEKRDELASGMRIILGEHRRLWSARNRAGGLQDSESIFDERLQEYTGAS"
     gene            complement(4674..5708)
                     /locus_tag="VFQ10_01695"
     CDS             complement(4674..5708)
                     /locus_tag="VFQ10_01695"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9964082.1"
                     /translation="MDLDGLLFSAASAEVLAGMLIASLRLDLPTVCAPSDSPPLSATL
                     IALGLAPGLGDPANMAVSLPEVEGPRPRKLVDDFSLANALRAGIAAGGGPEVLVHLAA
                     IAREAGVGGFGQMLRVLASETPQADPEWLREYGVPGLLSSLGDALHDVPTIAGSLKGN
                     LPPAPPAPGERSRLVFVRARASGAEAVCRVPEGVTDPAGECRVFGSEKEAVSGVLRGE
                     VGEGLLLVVGGCGPRGGPGLLRLDALAQSLKGAGLEVPVLTDGLAPEEALGAWTTLFT
                     PEAVAGGVLSLLRDGDTLRIDLAEGRIRTGAGDFDSREFGTFPNNAGTAYAARYARIA
                     LPALEGAGFA"
     gene            complement(5895..7346)
                     /gene="gatB"
                     /locus_tag="VFQ10_01700"
     CDS             complement(5895..7346)
                     /gene="gatB"
                     /locus_tag="VFQ10_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET9964083.1"
                     /translation="MKEKRTYQAVIGLEFHVHLATTTKMFCGCKVTYGDPPNTHTCPV
                     CLGHPGALPVTNEKAVELGVMAGLALNCDVADRAIFARKNYFYPDLPKGYQISQFDEP
                     ICTGGHIDVPTPEGKIRVGITRLHLEEDAAKNVHVGTTGRMHGSVGSLVDFNRGGTPL
                     MEIVTEPDIPSPEAARATANQLKDILRAVGVSEADMEKGQLRCDANVSIRNPDGTFGT
                     KTELKNMNSFRFVERGLARELERQREILEKGGRVEQTTLHYDPETDEVHELRSKEYAH
                     DYRYFPEPDLLPLELSRAYVREIEDRLPELPDHMLARFVEQYGLSEYDAGVLTSDRNL
                     ASFYEAVAAEVDPKQASNWISGDLRALLNETGTDISDTRVTPEHMKELIELVAAGTVS
                     RSAARTALDAVFETGDSPSAVVEREGLASVGSDELSDIVDGAISANPEEAERVKGGDK
                     KVVGFLVGQVMKATRGSADGRRVRELLLEKLGS"
BASE COUNT         1322 a         2451 c         2418 g         1452 t
ORIGIN      
        1 ctgagggtgt ccccagtatc gtccgttagc tggtagagca aacttcccag agactcgcac
       61 aagcccgaga actgggccac gaactcggcg ccctccccca ccggtacggg ggcgtttatg
      121 gcgtgtaccg gcacggcgcc cctgagggct gtcgccacct gctcggcagc cgtgatgcct
      181 gccctgtcct gggcctccgc ggtgctcgca ccgaggtggg gcgtcacaac cacgttgggg
      241 agcgtgaaga tcggggagtc cgtcgtcggc tcctcggcga atacgtcgag tgcggcaccg
      301 gcgatggcgc cctccttgag ggcgttgtag agggccgtct cgtcgacgat gccgccacgg
      361 gccacgttta tgaggtacgc ggtaggcttc atcttcgtca aggcttcttc gttcacgagc
      421 cctaaggtct ggggagtacg cggaacgtgt agcgagacgt aatcagcccg ctcgaagacc
      481 tcgtcagcgc tctctgccct cccgacgttc atcgagcgca tcctgtcctc ggagacatag
      541 gggtcatagg cgaggacccg catccccatc cccaatgcgc cccgtgccac gatggcgccg
      601 acgtgaccaa gaccgaccag cccgatcgtc ttctccgaca cctcgacacc tttgagcgcg
      661 ctccgcttcc actccccacg ccgcacggaa gagtccgcgg cagggatacg ccgcgccaca
      721 gcgagcatga gccccagggt atgctcggcc gcggcgaccg tgttgctctc aggggcgttg
      781 gcgacgatga tgccgtgctt ggtagcggcc tctatgtcta tgttgtcaac tccgatgccg
      841 gcgcgtccga tagccttgag gttcacggcc cgctctatta catcggcggt gactttcgtg
      901 gcgctgcgga tgatgaggcc gtcgtaatcc ccgatcttct ggagcagttc ttcagggctg
      961 aggtcgagga agacgtccac ctcgaagtcc tgctctagta gctctatgcc aggatcggcg
     1021 agcttttcgg ggacgaggac cctcaagccc tcaccccact cttcgagaag accctctggg
     1081 cggcgccgac acccttgcca agctccacgg ggtagtctag ggactccagc gccagctcgg
     1141 tggccgctat cgtggcgatg atgtcgaatg catcgaagta gccgcagtgt ccgatgcgga
     1201 agatcctccc cgccatctcg ccctgcccgc cggcgacctg tatcccatgc tcgcggaaga
     1261 tcatacgcac gagctgcttg ccgtcgatac cctcaggcac ccaggcagcg gtgacggccg
     1321 agttggggtc ctcgtcaggc ccgaagagct ccagccccag gcccttgatc cccgccctcg
     1381 acgcccgacc gagcagtacg tgccgctgga agacgttctc gatgccctcg tctatgagct
     1441 gctgcaacgc gacgtcgagc tggatgatga ggcttaccgc aggggtccac gccgtctggg
     1501 ccgggtcctt ctcgtaggcc ttcctggccg ccgtccagtc gaagtaatag cgtggcatca
     1561 gcgactcttc gtgcatccgc atggcccgct cgcttacgct aacgaacccc aggccaggag
     1621 gcgtcatgag cgccttctga gagccggcta cgacgacgtc gacgccccac tcatcggtcc
     1681 tcaacgtaca ggccccgagg cccgaaaccg catcgacgat ggtgacgacg tccgaggcgg
     1741 ccctcgcgaa gccctctatg tcgtttacgg tgcccgtcga ggtctccgag agcacgcaga
     1801 tagcggcctt gatctcgggg tcgttcgcca gcgcttcggc gacctcgtcg ttgtccgcct
     1861 tctggcccca atcgtactcc agctcggtga tctccaggcc gaaggcccgg ctcatcttga
     1921 cccaccggcc gccgaagttg ccgttgttta cgacaagaat cttgtctccc ggcgagaaga
     1981 ggttctgtat cgcgccctcg aaagccccgg tcccgctcgc cgcataagtg aagatatcgt
     2041 tctccgtcaa gaagacccgc ttcaaattct cgttcaccct ggtgaacacc tcgccgaact
     2101 cgggcgtgcg atggtggatg ataggtagcg cccccgccgc agagacctca ggaggtattg
     2161 gagtggggcc aggagacata agacggtact tgttcatcat gggtgctgag cttcctctct
     2221 caccgcgtac aacaggatca tattctagac ataatcgttg ccgatggcta cataatggga
     2281 catctctttt acaaagtgtt gctttcggcc tcgccacggt cgcggcagcc ggccgcggcc
     2341 cacttgttgc tcgcgggccg ccggcgagcg ctctcggtgg cagggtcggc gacggcccgg
     2401 acactcacgc cagtgtcgac agcctgcatc gaggcgatca tcgcggtagc ccggtatccg
     2461 agcccgataa cagcgagcct cgtcacgccc ctggatcaaa caccaatcgc ggctgctgcg
     2521 tagtatccca ggctggtgtg gcggaaacgc tgggagagaa gtcctggagc tgctcccatc
     2581 acctccaacc tacaacgatt gtgactctcg gcgcaagcga tacgccgcag ggtgatcggg
     2641 gacggatagg ggctgtgtat ctcacgaggc tcctgtgtat tcctgcagcc gctcgtcgaa
     2701 gatgctctcg ctgtcctgca ggccgccagc gcggttgcgg gcgctccaca ggcggcggtg
     2761 ctcgccgagg atgatacgca tccctgaggc cagctcatca cgtttttcgg cggcgtcgat
     2821 ggttccttgg agtattgccg tcccccgctc acaggcgtgc agcatcattc tcgctgcgtt
     2881 agcgaactcg tcgcggatga tccccgcgtc gaccctggcc atgcgcgcgc cttcgagcgg
     2941 ctcgattatg gagccgatgt gctcgccggc caccctgagg ctctcgaggg ttacaccatc
     3001 agggagcggg ctgcccgggg cctgcgtcag cagatggaag aggaccgtgg agttgtgcgg
     3061 gaccggtccc gccttctcgt gcgcgttgcc gaggtcgtgg ctcaacctgc ccatgacgcg
     3121 cgcctcgtcg tggaacgcgt gcaggtcgag ggcggcgata aagtcctcat ctctattggt
     3181 ctcgtggcgc cagggcagcg cggccccggc ggcgaacccg aggtagctta ccggcaggta
     3241 ctgtgtatgg tcgttgtcgc cccagtcggt gttgagcagc cctatcgccc cgtgcctgag
     3301 cccgttttcg gtcgcgtttc ggacgttggc caggcagatg tcggtacgac cggcgatcgt
     3361 gttccacgaa gaggtgccag gacatacgaa gaaaggtatg cccgaccgcg cgaactcggc
     3421 cccatgctcc tcgaacggat ggtcggcctc ataaccccac tcgagcacca cagaatcttt
     3481 cggcagttca ggcaccagat caggatgctg gatgatgatg tctccccagt agtgcatcgt
     3541 gcgacggtga tgcttgacca ggtcgtagat tccgagcagg tagtcgaggt agacgcggcc
     3601 cgcgcccttc tcctcgacgg cctgagcgct ccgtcccttc cccacgtccc aggtctcatc
     3661 gcagcccacg ttgaacttct cgctggagaa atgcggaagc aattcagaga acagctctcc
     3721 catgaactcg acgctgcgac ggtccgttgg gttcaggctg aagggcggca tctgcctggt
     3781 ctgcccttgc caggtaagct cgaagccgtc cgggcactcg gccaggtggt tgtatcgtgt
     3841 cctctccagc cagtggtgca tgtgcccgaa ggagttctgg ttcggcacca gctccacgaa
     3901 cctctcgcga caataggcgt cgagcctgag tatgtcctca ccggtcatcg ggctcgcctg
     3961 cgcccatacc tcgcggtggt tctcgtaggc gaaggtgtgc tccgtgtaca gttcgaggtg
     4021 gttgatcttc cacccggaga acaggtcgac cagatcgaag agcgtctgga gggtggggac
     4081 cttgctcctg ctgatatcga gcatcacccc gcgcgaaggg aaatcaggag agtcctcgat
     4141 ctcacccgca gggagcgccc cctcgctctg gcgcaacatc tgcaccagag tcatgacgcc
     4201 gtagaatgct ccggccgcgt caccggcaac gaggtctatg ccgtcgtcag caatccgcaa
     4261 ccggtagcct tgcgcgggga tgcccccctc tagcgcgagc gtcatggtcg tcccgcccct
     4321 ggtatcgtag attccgggat cccccgcccg caaggtccac ccgatacgtt ggttctccca
     4381 gagcgcccgc tgcaacctgt gggctatggg gaacaatgat ccaggatccc cctgacagat
     4441 gatcttcgag ccgccggtta gtctgtaggc gcccgtacgg gcgacgaccg agcgcggctt
     4501 tgggagcagg atcatgtctt cgatagttgc catgacttcg attggtttcc tctctgaacg
     4561 tccgttatcc caccaaaagc atgatgccac gtggtaaagt ccggcgcatc ctaggcaacg
     4621 cgcctcgggg taagatcgcg cgtgacggcg agaaagccgg ccgggattac aggctaggcg
     4681 aagccagcac cttccaacgc gggtagtgcg atccgcgcgt agcgggcagc gtaagccgtg
     4741 cctgcgttgt ttgggaacgt accgaactcc ctgctatcga aatctccggc cccggtgcgg
     4801 atgcggccct cagcaaggtc tatcctgagg gtatcaccat cgcggagcag gctaaggaca
     4861 ccgccagcca cagcttcggg ggtaaagagt gttgtccagg ctcccagagc ctcctccggt
     4921 gcgagtccgt cggtcagcac aggcacctcg agtcctgccc ccttcaggga ttgcgcgagc
     4981 gcgtcgagcc gaagtagtcc cgggccgccg cgcggcccgc aacctcccac cacgagcaac
     5041 aatccctctc caacctcgcc acgaagcacg cccgagacgg cctctttctc agagccgaag
     5101 acgcggcact ctcccgccgg atctgtcacg ccctcgggca cgcggcagac ggcctcggcg
     5161 cccgacgccc tggccctgac aaagacgagg cgagagcgct cccccggcgc aggaggcgcg
     5221 ggcggcaggt tccctttcaa gctgcccgcg atggtaggga cgtcgtggag tgcgtctccc
     5281 agggaagaga ggaggcctgg tacgccgtac tcccgcaacc attcagggtc ggcctggggg
     5341 gtctcggagg cgagaacccg caacatctgg ccgaatccgc cgacgccggc ttcacgagcg
     5401 atcgcagcga ggtggacgag aacctcaggc ccgcccccgg ccgcaatgcc ggcccgcagc
     5461 gcgttggcga gagagaagtc gtcaaccagc ttgcgagggc gtggcccttc aacttcggga
     5521 agtgagacag ccatgttcgc agggtccccg aggcccggcg cgaggccaag tgcgataagg
     5581 gtggcggaga gaggtgggct gtccgacggt gcgcagaccg ttgggagatc caggcgcaga
     5641 gaggcgatga gcattccagc gagcacctcg gcgcttgcgg cactgaacag gagaccgtcg
     5701 aggtccatgc tggagcagga gatctctacc aggtcagcga gccattcgcg ggccagcgca
     5761 acaccgcctt cgggtaccga gggttgtagg cgaaggggta ctggctctcc tcctgcgtcg
     5821 tggacggcgt cagggaggcc tgagggttct aggaaaggga cgccgatacg cggggctttc
     5881 aggaccgctg acctctagga gcccagcttc tccagaagaa gctctcggac ccttcggccg
     5941 tcggcgctgc cgcgtgtcgc cttcataacc tgcccgacga ggaagccgac caccttcttg
     6001 tcgccgcctt tgacacgctc ggcctcttcg gggttggcgg agatcgcccc gtccacgatg
     6061 tcggagagct cgtcggagcc tacagaagcg aggccctcgc gttcgacgac cgctgatgga
     6121 gaatcgccgg tctcgaagac cgcgtcgagg gccgttcttg ctgcagagcg cgagacggtg
     6181 cctgctgcga cgagttcgat cagctctttc atgtgctccg gcgtaaccct ggtgtcggag
     6241 atatctgtgc cagtctcgtt gagtagcgcg cgcagatcac cggagatcca gttagaagcc
     6301 tgtttgggat ccacctccgc tgcgaccgct tcgtagaacg atgcgaggtt tctatccgac
     6361 gtaagtaccc cggcgtcgta ctcagagagg ccgtactgct cgacgaagcg ggcgagcatg
     6421 tggtcgggca gctcggggag ccggtcctct atctctctga cgtaggcgcg cgagagctcg
     6481 agcggcagga gatcaggttc cgggaagtag cggtagtcgt gggcgtactc cttgctgcgg
     6541 agctcatgca cctcgtcggt ctcggggtcg tagtgaagcg tggtctgctc gacacggccg
     6601 cccttctcca ggatctcacg ctgtcgctcc agctcgcgcg cgaggcctcg ctcgacaaag
     6661 cgaaaggagt tcatgttctt gagctcggtc ttggtgccga aagtaccgtc ggggtttcgt
     6721 atggagacgt tggcgtcgca gcgtaattgc cccttctcca tgtcggcttc cgaaacgccg
     6781 accgccctca ggatgtcctt gagctgattg gccgtagcac gcgccgcttc gggcgatggg
     6841 atgtcgggtt cggtgactat ctccatcagg ggcgtgccgc cgcggttgaa gtcgacgagg
     6901 gagccgacgg acccgtgcat gcggcccgtg gtaccgacgt gtacgttctt cgccgcgtcc
     6961 tcctcgaggt gaagccgcgt gatccccacc cggatcttgc cttcaggggt agggacgtcg
     7021 atgtggcctc ccgtacagat cggctcgtcg aactgggaga tctggtaccc cttgggaagg
     7081 tcggggtaga agtagttctt cctggcgaag atcgcgcggt ctgcgacgtc gcagttgagc
     7141 gcgagacctg ccatcacccc tagctcgacg gccttctcgt tggtgacggg cagggcccct
     7201 ggatggccga ggcacacggg gcaggtgtgg gtgttcgggg gatcgccgta ggttacctta
     7261 cagccacaga acatcttggt cgtggtcgcc aggtggacgt gaaactcgag ccctatgacc
     7321 gcctggtagg tacgcttctc tttcacttcc aaatgccagc ccccatgcca gattcgcctg
     7381 ccgtcgtaac ctcattctag catcgtcggg catcctgccc ggcctgtggg gcggatgtta
     7441 gaatgcttct tacggaggtt tgcgtaagct tgcataccga gatagagaac gccgggggta
     7501 accggccgca cctcgtggtc ttgcaaggac ccagtctcgg cgacttcttc gagataaccg
     7561 acgacgagct cgttcttggc agcgacccgt tctcggctga catcgtggtg cgggacgtgg
     7621 aggtcgaacc gcggcacgcc gaa
//
LOCUS       DASTKM010000044        12536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_536378, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000044 DASTKM010000000
VERSION     DASTKM010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12536
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536378"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFQ10_01705"
     CDS             <1..540
                     /locus_tag="VFQ10_01705"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HET9964084.1"
                     /translation="TPKAVEESRAIVLKLDEERMRASLVREYTHPDKQYADAAGNMQV
                     LPNGNVFVGWGRGLAISEFSHEGELLFDLRVSPEHRSYRAFRLPWSGSPSDQPACVAE
                     RTSENELEVYASWNGATDIATWEVLAGPHPGQLESLGSVPRNGFETAMLVKTSEPYVA
                     VQARHRSGRVLGASTAVEA"
     gene            complement(685..1350)
                     /locus_tag="VFQ10_01710"
     CDS             complement(685..1350)
                     /locus_tag="VFQ10_01710"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET9964085.1"
                     /translation="MSEDRFEELLGPYLLGELSVEEERELERHLEECSGCRDELARAR
                     QTHDLLRELAAGGPPELKNRVLAQVRGESPARSRGGWRLWVPAAAALVVAVIGAGLLW
                     AITEDSSDGIPLTATVPASEGGGELSGEMEGGNLQIELVVWDLPELRKHEYYELWYAK
                     EGDGRISCGTFRAQQGGQTTVNLTAPANAVSYPEIEVTREPDDGDPGSSGEKVLVGDL
                     RSL"
     gene            complement(1343..1915)
                     /locus_tag="VFQ10_01715"
     CDS             complement(1343..1915)
                     /locus_tag="VFQ10_01715"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9964086.1"
                     /translation="MFGPVVGAVEDEELIRRVAEGDDRALSELYDRYSRPVYATGISM
                     LGDASLAEELVQDAFMSVWRAAGSFDPGRARFATWLYRITRNRAVDLNRRRQVRPFSA
                     GEELLRNVSGGPEPEGGVDGWDVARALSRIPHEHREVLTLAYFEGLSQREISRRTGVP
                     LGTIKSRTTAALKRLHRSLVNPAPEESRHE"
     gene            2235..2798
                     /locus_tag="VFQ10_01720"
     CDS             2235..2798
                     /locus_tag="VFQ10_01720"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HET9964087.1"
                     /translation="MASDRNDERLAAPGDLELVRDFVNTLDILPGADKFADPAGLALW
                     LADHRLVPLSRALTDKDLSRAKKLREALRAFLLANARLPLAPEAAEAFDDAAGPAHLR
                     ARADERGGLELLPAEEGGLDHAIGRLVAIVFAAQQDGTWPRLKACAECHWALYDHTKN
                     HSAAWCGSQCGARVRSRRYRQRHGSPA"
     gene            complement(2821..3645)
                     /locus_tag="VFQ10_01725"
     CDS             complement(2821..3645)
                     /locus_tag="VFQ10_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET9964088.1"
                     /translation="MNGREITTAAIQMSSTPNKGENLDTAERLMRSAASSGACLIALP
                     ELWNCHGLEDVYRENAEPVPGPTTEFLGGLAQELGVYLIGGSILEGDPSSTKLHNTST
                     FFGPDGRMSAVYRKIHLFDVKAPDREYLESRTMAAGTEIVTAKAGAATIGLSVCYDVR
                     FPELYRLLALRGAEVLAVPAAFTLQTGKDHWELLLRARAVENQAYVAAPAQWGRKADG
                     RWTYGRSIIVDPWGTVLSQCPDRDGYALATLDLDYLDTFREQFPALKNRHPEAYDW"
     gene            complement(3685..4155)
                     /locus_tag="VFQ10_01730"
     CDS             complement(3685..4155)
                     /locus_tag="VFQ10_01730"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase
                     domain-containing protein"
                     /protein_id="HET9964089.1"
                     /translation="MLIYLAGPLFSEAERRFNLGLAHRLEALGFDVFLPQRDGVKRDR
                     PPYDTMTPEERRLAMFHLDRTRILDAEVFLFVLDGRVPDEGACVELGIAYCQKYLQDG
                     EKLLVGLHTDPRAAFIGGRLNPMVRVPLEYVVDDEEKLLRVLAGYGSGVEAGTE"
     gene            complement(4180..5091)
                     /gene="ftcD"
                     /locus_tag="VFQ10_01735"
     CDS             complement(4180..5091)
                     /gene="ftcD"
                     /locus_tag="VFQ10_01735"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET9964090.1"
                     /translation="MRPPLFEAVPNFSEGKDATKISRITEAVRAVSGVRILGLHSDPD
                     HNRSVLTFAGGEDSVLEGALVLAKACAAELDLASQTGEHPRMGVLDVLPFVPLEGATM
                     DDAVRLAHLAGEILGSLGFPVFYYGSAATASHRRSLPDIRRGGYEGVAFRLGDPLWQP
                     DSGPKTLDPSRGAVAVGARPFLVAFNAYLDTSDVEVARAVATRVRESGGGLPAVRALG
                     FGVGGKAQVSMNLTDLEQTPIPVAFEAVRSAAADLGASVESTELVGLAPLEAILQTAR
                     YYLGLRELNAQHVLETRLYGPSDSRSL"
     gene            complement(5098..6894)
                     /locus_tag="VFQ10_01740"
     CDS             complement(5098..6894)
                     /locus_tag="VFQ10_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET9964091.1"
                     /translation="MGYLPIEDHGVIGNLYTAALVGTDGTIDWLCLPAFDSPSVFCSI
                     LDEEKGGHFALRPVEYTHSHQLYLPDTNVLLTRFFSPAGLAETLDFMPIVTRPVERHR
                     LVRNVRVIRGKIHFRVECFPAFDYARRDHTVSVGKSGAVFASGDHCLGLATDVRLEKG
                     AEGAAGTHFALGEGETATFVLTHLRRGEAPGEILSDVGFEELLHGTLGYWRRWLSNST
                     YQGRWREIVHRSALVLKLMVYDPTGALVASPTMGLPERIGGERNWDYRYTWLRDAAFT
                     LYALIYVGFEHEARHFMDWLRDRCHEDEDGLLQPLYGIDGRKDITETELWHLSGYMNS
                     SPVRLGNGAFKQRQHDLYGAVLDAAYLYNKHGAPLDYDLWKNLRKILNWLSENWRDPD
                     EGIWEVRGGRRQFVSSKLMSWVALERAGRISRQRGLPSGDGRWMAERDAIYEEIMEKG
                     WNPEKNSFVQYYGSYALDASLLMMPLVKFVGPTDPRWLSTLERIQQNLAHDTLVKRYQ
                     PEYAAPDGLEGDEGSFSLCSFWFVECLTQAGRLDEARLALEKMFSYANHLGLYAEEIG
                     HSGEALGNFPQAFTHLALISAAVHLDRALGAR"
     gene            complement(7117..7539)
                     /locus_tag="VFQ10_01745"
     CDS             complement(7117..7539)
                     /locus_tag="VFQ10_01745"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9964092.1"
                     /translation="MRSTLILAVLAGVCVAVQTSLTGAAQRTLGPLVLVAISGLTTGV
                     FALLISLVVAKPEFTGRAVGYAVTSGVLGAVIVGSIAVAAGQAGVARALSLVIAAQLI
                     AGLVLDALGIFGAGADFSILKALGVLLIIAGGALVVSY"
     gene            7705..8421
                     /locus_tag="VFQ10_01750"
     CDS             7705..8421
                     /locus_tag="VFQ10_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET9964093.1"
                     /translation="MLGGARITFLGHATFRFVTAGGGQIIIDPFLTENPQTPDDMKHV
                     GHLDTILITHGHFDHFSDVESLAGQTGAATVSNFEIFSYLQREGIENAIPIQKGGTIE
                     VGGLKVTATNAFHSSSIQLDDGSYIYAGEPMGYVIEFESGFKLYHAGDTAVFGDMRLI
                     GEIYNPDLALLPIGNQVVMSPLEAAHAARLLGVEHVVPMHYGTFPFLPGTPEEFQEHV
                     SALVPDLKVHVMKPGADLSS"
     gene            complement(8472..9203)
                     /gene="cobS"
                     /locus_tag="VFQ10_01755"
     CDS             complement(8472..9203)
                     /gene="cobS"
                     /locus_tag="VFQ10_01755"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HET9964094.1"
                     /translation="MKGLLSFFTVLPVRGASLEEAAREVRLLPLIGLLTGLPGAVLLL
                     AAHIVPPGVSATLALGAVLLAAGLHHADGVLDVGDAIMAHGSPDRRRDVLEDTRVGIG
                     GLGALFVVYAPTVGALAALCAISPARAALALLAGEVAARSAMLLMLAFGEPADPRSSS
                     VPFVRALSGPHRTPAVVVALLAPLPFLLATSALAPLAIFAAPLVAIGALRISATKFGG
                     IGGDVVGATGEVSRAALLVLLSATI"
     gene            complement(9204..9686)
                     /locus_tag="VFQ10_01760"
     CDS             complement(9204..9686)
                     /locus_tag="VFQ10_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HET9964095.1"
                     /translation="MLGGARSGKSAFAERLASSLGGERVLYVATAVEDGDEDLQRRIR
                     LHRERRPCGWGTLEIGGLDEVLGAAAKWDAVLLDSLTLWVSARDEPALPEFDRFLAGA
                     GDLSVPLLLVSDEVGLGVVPESDAGRRFRDVLGLVNQRAAEAAKEVHLCIAGVGVRIK
                     "
     gene            complement(9701..10771)
                     /gene="cobD"
                     /locus_tag="VFQ10_01765"
     CDS             complement(9701..10771)
                     /gene="cobD"
                     /locus_tag="VFQ10_01765"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HET9964096.1"
                     /translation="MVRGWAQHPGDRHHGAHGDLVSRSLDVRAGELLDFSQNINPLGA
                     PRNALEAARQALYEDSGRYPDLEYSGLREALAAYLGVGAEMVVPTNGGAEALFLAARA
                     AATGGRALVLEPTFSEYAAAARASGMEPVRKVARRREEGFGWDPTSYRDLEGVSVVFL
                     CNPNNPTGDMLGREEVLEVAACVEEAGAVLVVDEAFADFVPEISVTDMVDRGLWVARS
                     FTKFFAIPGLRLGCLVCHDAARVQALQPSWPVNAVAAAAGIAAVRDRGFAEASKVEVA
                     RLREDLFGTLDALPGLRPFPGAANFLLVRGTDGLPGRLARRGVLVRGCGPFYGLGPEY
                     FRVAVRSPVENGLLVEAIKEEL"
     gene            complement(10764..11699)
                     /gene="cbiB"
                     /locus_tag="VFQ10_01770"
     CDS             complement(10764..11699)
                     /gene="cbiB"
                     /locus_tag="VFQ10_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HET9964097.1"
                     /translation="MSPQRGGQVAAALLLDGVLGEPPETIHPTVLMGLVISAFEKRAL
                     RLESPRSRRLAGIVLAVSTPSLVFVSTRKILGFVPCKPRWMIGTALISTTLSMSGLAE
                     AAQKVETGLRDGRLERARTQVGHLVGRDTGDLSESEVCRAAVESVAENTSDGVVAPML
                     YGVLFGAPGALAYKAVNTLDSMVGYRQPPYEDLGWASARLDDLANLAPSRLTMLSTAA
                     VAGRPLRTLLIAVRYGPLTASPNAGMAEAAFAGALGIRLGGANTYGGVLREGPILGDG
                     RLPAPDDIGRAVRLMRRCCVLAGILLFLAERVFRG"
     gene            complement(11696..12370)
                     /locus_tag="VFQ10_01775"
     CDS             complement(11696..12370)
                     /locus_tag="VFQ10_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964098.1"
                     /translation="MYGQPRRVSGPIRTGYFYGLAGVLVALASDLYFLFVDPVHTPGW
                     VLAAIAQYRTRLALALFLFLAILAALRVRPTRLDHGVPYRSLLLRDGALAATVVAVMV
                     GITLFLTTALQATLLAGAARDYAREAAPKIVSYVNEVRMEIRQERKARGASPEKVRDL
                     PPPAQVEEVEATLQPPGLRDLGRSMANFVLRAVLLGTAGGLVGALRGRPALSDNEKPR
                     GAPRRE"
BASE COUNT         2305 a         4133 c         3979 g         2119 t
ORIGIN      
        1 acccccaaag cggtagaaga gtcccgcgcc atcgtgctga agctcgacga ggagcggatg
       61 cgtgcctcgc tggtgcgcga gtacacccac cccgacaaac agtacgccga cgcggccggc
      121 aacatgcagg ttttgcctaa cggcaacgtc ttcgtaggat ggggaagggg actggccatc
      181 tcggagttca gccatgaagg ggagctgctc ttcgatttga gggtctcacc cgagcacagg
      241 tcctaccggg ccttccgcct tccgtggagc ggcagcccct cagatcagcc cgcttgtgtg
      301 gcggagcgca cctccgagaa cgaactggaa gtctacgcta gctggaacgg agccacggat
      361 atcgccacct gggaagtgct cgccggcccg caccccggcc aactagagtc cctgggatcc
      421 gtcccccgga acggattcga aacagccatg ctggtgaaaa cctccgaacc gtacgtcgcc
      481 gtgcaagcca gacatcgttc gggacgagta ctcggcgcct caacggctgt cgaagcatga
      541 ggcaagtcag catgtcggca catcgcctgc tgcggctctc agcatctcag caagtcctgg
      601 cggcgctccc gaaccgggac gccgaggctt tgtgcaggag gctttcctcg atgcgtcgct
      661 cgtcaatgct gacatgctga gagctcatag acttcgaaga tctccgacca ggactttctc
      721 accactggag ccggggtcac cgtcatctgg ttcgcgggtc acctcgatct ccgggtacga
      781 cacagcgttg gcaggagccg tgaggttcac ggtcgtctga cctccctgct gcgcccggaa
      841 ggtgccgcag ctgatccgtc cgtcaccttc tttggcgtac cacaactcat agtattcgtg
      901 cttccggagt tcgggcaggt cccacaccac gagctcgatc tgaaggtttc ccccttccat
      961 ctccccgcta agctcgccgc cgccctccga cgcgggaacc gtcgcggtca agggaatgcc
     1021 gtctgaggag tcttctgtga tcgcccacag gagtccggcg ccaatgacag cgacgaccag
     1081 cgccgccgcc gccggaaccc agagcctcca tccgcctctg gagcgggccg ggctctcgcc
     1141 cctgacctgc gccagtacac ggttcttgag ctccggcggc ccgccggcgg ccaattctcg
     1201 gagaaggtcg tgagtctggc gagcgcgagc cagctcatcc cgacatccgg aacactcctc
     1261 tagatgacgc tcaagttcgc gctcctcctc gacggatagc tcgcctagca ggtaggggcc
     1321 gaggagttcc tcgaagcggt cttcactcat gtcgagactc ctccggggcg gggttgacca
     1381 ggctccggtg gagcctcttg agggcggccg tggtccggct cttgatcgtc cccaaaggga
     1441 cgccggtccg gcgggagatc tcgcgctgtg agagtccttc gaagtacgct agtgtcaata
     1501 cttccctgtg ctcatgaggg atgcgcgaga gcgccctggc gacgtcccaa ccgtccaccc
     1561 ccccctcggg ctcagggccg cctgagacgt ttcgtaggag ctcctcgccg gcagagaagg
     1621 gccgtacctg ccgcctgcga ttgagatcca cagcccggtt gcgggtgatc ctgtagagcc
     1681 aggtcgcaaa cctcgcccga cccgggtcga aggaccccgc cgcccgccag acactcatga
     1741 aggcgtcctg gaccaattcc tcggccaggg aggcatcccc aagcatgctg atgcccgtgg
     1801 cgtataccgg acgcgagtaa cggtcgtaga gctccgagag cgcccggtcg tccccttccg
     1861 ctacccgacg tatcaactct tcgtcctcca cagctcccac taccgggcca aacatctcct
     1921 tcccaccccc gagtcccgaa ctagcagttc aaaccgccat tcggtcaatt cccggatcca
     1981 cctcggcgac cgcagaacca cctttagggt tcgaccgagc cgctcatggg cttgcgtcca
     2041 ggacaggggg tggttccctc cacagcgacc tcgccgatct ctataacggt ttgtacgcaa
     2101 ctacgcagcc cgatggttcg tttcaggaaa ttatgcagcc tgagtgtgtt gtaattatgg
     2161 tcaaaccgtt ttagagtgtg gcgtgatgag ttcttcggcg ttcggggcga gggcggtcag
     2221 gatagaatgt cctcgtggca agcgatagga acgacgagcg actggcggcc ccaggagacc
     2281 tggagctcgt ccgcgacttt gtgaacaccc tggacatact gccaggcgcc gacaagttcg
     2341 cggacccggc tggtctcgct ctctggctgg ccgaccaccg gctcgtgcct ctctcccgtg
     2401 cccttaccga taaggacctc tcgcgggcga agaaactgcg cgaagcgctg cgtgcgttcc
     2461 tcctggccaa cgcgcgtcta ccgctcgccc ctgaggcggc tgaggcgttc gacgacgctg
     2521 ccggccctgc acacctgcgc gccagggccg atgagagagg tgggctggag ctgctcccgg
     2581 ccgaagaggg ggggctggat catgccatcg gccgattggt tgcgatcgtg ttcgcggcgc
     2641 agcaggacgg aacctggccc cgcctcaaag cttgcgcgga gtgccactgg gcgctctacg
     2701 accacaccaa gaaccactcc gccgcctggt gcggctccca gtgcggagcg cgggtgcgtt
     2761 ccaggcgata ccgccagcgc cacggctcgc ccgcgtagag ggtcaggggc gtggactctc
     2821 ttaccagtcg taagcctcgg gatggcggtt cttcagggct gggaactgct cccgaaaggt
     2881 gtcgaggtag tcgaggtcca gggtggcgag cgcgtatccg tcgcggtcgg ggcattggga
     2941 gagcacagta ccccacgggt cgacgatgat tgaccgtccg taggtccatc gtccgtcggc
     3001 cttccttccc cattgggccg gagcggcgac ataagcctgg ttctccacgg cccgcgcccg
     3061 caacagaagc tcccagtgat ccttgcccgt ctggagcgtg aaggccgcag ggacggcgag
     3121 tacctcggcg ccgcgcagcg ccagcaatcg gtagagctcg gggaagcgta cgtcgtaaca
     3181 gacagagagc ccgatggtcg cggcgccagc cttcgcggta actatctccg tgccggccgc
     3241 catggtccgg ctctccaggt attcgcgatc cggggccttc acgtcgaaga ggtgtatctt
     3301 gcggtaaacg gccgacatcc tgccgtcagg cccgaagaaa gtggatgtat tatgcaactt
     3361 cgtggatgag ggatctcctt ccaggatcga acccccgatc aggtagacac ccagttcctg
     3421 cgcgaggccg ccgaggaact ctgtcgtcgg ccctggtact ggctcggcgt tctccctgta
     3481 gacatcctcc aggccgtggc agttccacag ctcaggcagg gctataaggc aggcccccga
     3541 ggatgcggcc gaacgcatga gccgttctgc cgtatcgagg ttctcccctt tgttcggggt
     3601 cgaagacatc tgaatcgccg ccgtcgtgat ctcgcgcccg ttcatcgcac cgatcctctc
     3661 tacgccagta ggctgcttgc tgggtcattc agtacccgct tcgacgcctg acccataccc
     3721 ggcgagtacg cgcagtagct tctcctcgtc gtcgactaca tattctagcg ggacacgcac
     3781 catagggttc agtcttccgc cgataaaggc cgcccggggg tcggtgtgga gtccgacgag
     3841 gagcttctcg ccgtcctgga ggtacttctg gcagtaggcg atgccaagct ccacacatgc
     3901 tccttcgtca gggacgcgtc cgtcgagcac gaacaggaaa acctctgcgt cgaggatcct
     3961 ggtcctgtcg aggtggaaca tggcgagacg ccgctcctcg ggggtcatcg tatcgtaggg
     4021 cggcctatcg cgcttgacac cgtcgcgttg cgggaggaaa acgtcgaagc caagggcctc
     4081 gagcctgtgc gcaagcccga ggttgaagcg cctctcagcc tccgagaaca gtggtcccgc
     4141 caggtagatc agcaagcccg acacctccgg cgcatgcctc tacaaactcc gagaatcgga
     4201 cggaccatag agcctcgtct ccaggacatg ctgggcattc agctcccgca acccgaggta
     4261 gtaacgggcc gtctgcaaga tcgcctccag cggtgccaag ccgacgagct cggtggactc
     4321 taccgaggcg ccgaggtcgg ccgccgccga gcgaacggct tcgaaggcca cagggatggg
     4381 ggtctgttcg aggtcggtca ggttcatcga gacttgagcc ttgccgccga caccgaagcc
     4441 gagcgccctc acggccggaa ggccgccacc gctctccctg acccgtgtgg ccaccgctcg
     4501 ggcgacctcg acgtcgcttg tatccaggta cgcgttgaag gcaaccagga aaggacgggc
     4561 gccgacggcg acggcccctc tgctggggtc tagtgttttc ggaccggaat ccggctgcca
     4621 cagggggtcg ccaagccgga atgcgacacc ctcgtaacca ccccgtctaa tgtcggggag
     4681 ggatcggcga tgcgaggcgg tcgcggcaga cccgtagtag aaaacaggaa atccaaggga
     4741 acccaggatc tcgccggcca ggtgtgccag cctgacggca tcatccatcg tggcgccttc
     4801 caggggcacg aaagggagca cgtcgagcac gcccatcctg gggtgctcgc cggtctggga
     4861 ggcgaggtcg agttcggcgg cgcaggcctt cgccaggacg agggcgccct cgagcaccga
     4921 gtcttccccg ccggcgaaag tgaggacgct cctgttgtgg tcggggtcgg agtggaggcc
     4981 gaggatacgg acacctgaga ccgcgcggac ggcctctgtg attcgtgaga tcttcgtggc
     5041 gtccttcccc tcgctgaagt tagggacggc ctcgaaaagc ggagggcgca cggcggttca
     5101 gcgtgctccg agcgcgcgat ccaggtggac cgcggcggat atgagcgcta ggtgggtgaa
     5161 cgcctggggg aagttgccga gggcctcgcc cgagtgcccg atttcctcgg cgtagagacc
     5221 cagatggttg gcgtaggaga acatcttctc cagggcgagg cgcgcttcat cgaggcgtcc
     5281 tgcctgcgtg aggcactcga cgaaccagaa ggagcacaga ctgaagctgc cctcgtcccc
     5341 ttccaggccg tctggggctg cgtactctgg ctggtaacgc tttacgagcg tgtcgtgggc
     5401 caggttctgc tggatacgtt cgagggtgga gagccagcgc gggtccgtgg gcccgacgaa
     5461 cttgaccagc ggcatcatga gcagggaggc gtcgagtgcg tacgagccat aatattggac
     5521 gaagctgttc ttctcggggt tccagccctt ctccatgatc tcctcgtaga tcgcgtcgcg
     5581 ctcggccatc cagcgcccgt cccccgaagg cagtccgcgc tggcgggaga tcctgcctgc
     5641 ccgctccagt gccacccagc tcatgagttt agaggagacg aactgccgcc tgcctcctct
     5701 cacctcccag atgccctcat ctgggtcccg ccagttctcg ctcagccagt tcagtatctt
     5761 cctgaggttc ttccagaggt cgtagtcaag gggggcgccg tgtttgttgt agaggtaggc
     5821 tgcgtcgagc acggccccgt agaggtcgtg ctggcgctgc ttgaaggcgc cgttgcccag
     5881 ccttacaggg gaggagttca tgtatccgga gagatgccag agctcagtct cggttatgtc
     5941 cttgcggccg tcgatgccgt agagcggttg aaggaggccg tcctcgtcct cgtggcaacg
     6001 gtccctgagc cagtccatga aatgtcgcgc ctcgtgctcg aaaccgacgt agatcagagc
     6061 gtagagggta aatgccgcgt ccctaagcca ggtgtaacgg tagtcccagt tcctctcgcc
     6121 cccgatccgc tcgggcaggc ccatcgtggg ggaggcgacg agcgcgcccg tcgggtcgta
     6181 gaccatgagc ttgaggacaa gcgcggaacg gtggactatc tccctccacc gtccctggta
     6241 ggtgctgttg gagagccagc gtcgccagta accgagggtg ccatgcagca gctcctcgaa
     6301 cccgacgtcg gagagtatct ctcccggagc ctcgcccctc cggagatgcg tcaggacgaa
     6361 agtcgccgtc tccccctcgc cgagcgcgaa gtgcgtaccc gcagcgcctt ccgccccttt
     6421 ctccaaccgc acgtcggtcg ccagcccgag acaatggtcc cccgacgcga agacagcccc
     6481 tgacttgccg acggagaccg tatggtcgcg gcgggcatag tcgaaggctg gaaagcactc
     6541 gacacggaag tgaatcttcc ccctgatgac ccgcacgtta cgcacgagcc tgtgacgctc
     6601 caccgggcgc gtcacgatcg gcatgaagtc aagggtctcg gcaagtcccg caggcgagaa
     6661 gaagcgggtc aggagcacgt tggtatcggg caagtagagc tggtggctgt gggtgtactc
     6721 cacaggtcgc agcgcgaaat gtccgccctt ctcctcgtcg aggatggagc agaagacact
     6781 cggggagtcg aaggcgggaa ggcacagcca gtcaatggtg ccgtcggtac cgacgagcgc
     6841 cgccgtgtag aggttcccga tgacaccatg gtcctctata ggcagatacc ccactccagc
     6901 ctcccttcta agcatcgatg tccgtgcctg tatcttacag tgccgtacat aaggcttgcg
     6961 ccacatgtac gacgagcatg tcagctagtc agcacggcgc ctgttccagg cgacttgtca
     7021 gctttccgtg tgcgagacct ggcctatagg cggcgcagcc tccgggctaa agtgctgact
     7081 agcgaaggcc gcaaggcccg agcgtgctga aaggctctaa tagctgacga ccagggcccc
     7141 gccggcgatg atcaggagga ctcctagcgc cttcaggatg ctgaaatcgg ccccggcacc
     7201 gaagatccca agggcgtcga gcacgaggcc tgcgatcagc tgggcggcta tgaccagcga
     7261 gagagcgcgc gccacaccgg cctgtccggc cgcgacggcg atggagccta ctatcacggc
     7321 cccgaggacg ccggaggtca ccgcgtaacc taccgcgcgg cccgtaaact ccggtttcgc
     7381 caccaccaac gagatcaaga gcgcgaaaac accggtcgta aggcctgaga tcgcgaccag
     7441 gaccaggggc cctagcgttc gctgggccgc ccctgtcagg ctggtctgga cggccacgca
     7501 cacgcccgca agtaccgcga ggatgagggt agatctcatg ctgtccccct gaagttcgtc
     7561 gtctcaatgc agggccccgg tcgggacgcc cgcaggatta tacgcgggtg gcgaccgttt
     7621 ctccggtttg tgaggtgggt atacatctgg ttgttctttt cttgatcgtg tttggtgaac
     7681 gtacggagga taatcgtgga acagatgcta ggcggagcaa ggatcacctt cctcgggcac
     7741 gcgacgttca ggttcgtgac ggcgggcggc gggcagatta tcatagatcc attcctcacg
     7801 gagaaccccc agacccccga cgacatgaag catgtcggtc atctggatac catcctgata
     7861 acccacggcc acttcgacca cttctcggac gtagagtctc tggcggggca gacgggcgca
     7921 gcgacggtct ccaacttcga gatcttctcc tacctgcaaa gggaggggat cgagaacgcc
     7981 atacctatcc agaaaggcgg cacgatcgaa gtcggcggtc tcaaggtaac cgcgaccaac
     8041 gcatttcatt cttcgagcat acagctcgac gacggcagct acatctacgc tggcgagccg
     8101 atgggctacg tcatcgagtt cgagagtggc ttcaagctgt accacgcggg cgacactgcg
     8161 gtcttcggcg acatgcgcct tatcggggag atctacaacc ccgacctcgc cctgctgccg
     8221 atcggcaacc aggtggtgat gagcccgctg gaggcggccc acgcagccag gctcctcggc
     8281 gtagagcacg tcgtcccgat gcactacggt acctttccgt tcctgccagg cacccccgag
     8341 gagttccagg agcacgtctc cgccctcgtc ccagatctca aggtccacgt catgaaacct
     8401 ggtgccgact tgtcgtctta gcgatcagcg gttgacgatc agctttctgc cccggctaac
     8461 ccgagccggg gctatatcgt tgccgatagc aagaccagca acgcggcgcg ggacacctcc
     8521 cctgtagccc cgactacatc accgccgatc ccaccaaact tagttgcgga gattcgtagg
     8581 gcaccgatcg cgaccagggg tgccgcgaag atggcgagcg gcgcgagggc gctggtggcc
     8641 agaaggaatg gcagcggcgc cagcaacgca acgacgacgg cgggagtacg gtgtggcccg
     8701 gagagcgccc ttacaaaggg cacggaggag gagcgcggat ctgcgggctc gccgaatgcc
     8761 agcatgagga gcatcgccga ccgggccgcg acctcacccg ctaacagggc gagggcggca
     8821 cgagctggag agatagcgca aagcgcggct agtgcgccca cggtaggagc gtagaccacg
     8881 aagagcgcgc cgagaccgcc gatgccgacg cgggtatctt cgagcacatc gcggcgccgg
     8941 tcgggactcc cgtgggccat aatcgcgtca ccgacgtcca gaacgccatc ggcgtggtgc
     9001 agaccggcgg cgagaagcac ggcccctagc gcgagcgtgg cgcttacacc aggaggcacg
     9061 atgtgggccg ccagtagcag gaccgctcca ggcaatcccg taaggagacc gattagtggc
     9121 aacaggcgga cctcacgcgc ggcctcttcc agagaggccc cgcgaaccgg aaggactgtg
     9181 aaaaaagaca ggagaccctt caactacttg atcctgacgc caaccccggc gatgcagagg
     9241 tggacctcct tggccgcctc ggccgcccgc tggttgacca gcccaagcac gtcgcggaac
     9301 ctcctccccg cgtcgctctc cggcacgacc ccgagtccga cctcatcgct cacaagcaag
     9361 agaggcacgc tgagatctcc tgccccggcc agaaaccgat cgaactcagg cagcgccggc
     9421 tcatccctgg ccgaaaccca cagggtgagg gagtcgagca ggaccgcatc ccacttcgcc
     9481 gccgcgccca ggacttcgtc gaggcccccg atctccagcg taccccaccc acaaggcctg
     9541 cgctccctgt ggagccggat gcgcctctgc aggtcctcgt ccccatcctc aacggccgtg
     9601 gccacataga gaacacgctc gcctcccagc gaggatgcca ggcgttcggc gaaagcgctc
     9661 ttcccactgc gggcgccgcc gaggatgagg gcgcggacgg ctacagctcc tccttgatcg
     9721 cctcgacgag cagcccgttc tccactgggc tcctgaccgc gacgcggaag tactcaggtc
     9781 cgagcccata gaagggccca caaccccgca cgagcacccc cctcctggcg agccgccccg
     9841 gcagtccatc ggtcccccgg acgagcaaga agttcgcggc ccccgggaaa ggtcgtaggc
     9901 cggggagggc atcgagcgtc ccgaagaggt cttcgcgcag gcgggccacc tcgactttcg
     9961 aggcttcggc gaaacccctg tctctgactg cggcgatccc cgccgcggcg gcgacggcgt
    10021 tcaccggcca ggaaggctgc agcgcctgga cgcgtgcggc gtcgtggcac acgagacagc
    10081 cgagccgcag gccgggtatc gcgaagaact tggtgaagga ccgcgcaacc cagagccctc
    10141 ggtcgaccat gtctgtgacg ctgatctctg gaacgaagtc ggcgaaggcc tcatcgacga
    10201 ccagaactgc gccggcctcc tctacacagg ccgccacctc gaggacttct tcgcggccga
    10261 gcatgtcacc cgtagggttg ttcgggttgc agaggaacac cacagagaca ccttcgaggt
    10321 ctctgtagga ggtgggatcc cacccgaaac cctcctcgcg cctcctggct accttgcgga
    10381 cgggctccat gcccgaggcc cgggccgccg ccgcgtactc cgagaaggta ggctcaagca
    10441 cgagcgccct tccgcccgtt gctgccgccc tggctgcgag gaatagcgcc tccgccccgc
    10501 cgttggtcgg cactaccatc tcagcaccca cgccgaggta ggcggccagc gcttcgcgca
    10561 acccgctata ttccaggtcg ggatagcggc ctgaatcctc gtagagggcc tgcctggcgg
    10621 cttccagagc gttacgcggc gcgccgaggg ggtttatgtt ctggctgaag tcgagtagct
    10681 ccccggcacg gacgtcgagg gaccgcgaga cgaggtcgcc gtgggcaccg tggtgacggt
    10741 cgccggggtg ctgcgcccag cccctaacca cgaaacaccc tctccgccag gaacagcagg
    10801 attccggcca acacacagca gcggcgcatc aggcgcaccg cgcgcccgat atcgtcagga
    10861 gcgggaaggc gtccatcgcc gagtatcggt ccttcccgca gcaccccgcc gtacgtgttg
    10921 gcgcctccca gcctgatacc gagggcgccg gcgaatgcgg cctcggccat cccggcgttg
    10981 gggctggcgg tgagtgggcc gtagcgaacg gcgatcagga gcgtgcgaag tggccgtccc
    11041 gcgacagccg cggtggagag catcgtaagc ctcgacggcg ccaggttggc gagatcgtcc
    11101 agacgggcag aggcccatcc gaggtcttcg tagggaggtt gcctgtagcc aaccatcgag
    11161 tccagcgtat tcacagcctt gtatgccagc gcgcctggcg caccgaagag cacgccgtag
    11221 agcatggggg ccacaacacc gtcgctcgtg ttctctgcaa ccgattccac ggcggccctg
    11281 cagacctcgg attccgacag gtctcccgta tcccgcccga cgaggtgacc cacctgcgtt
    11341 cgcgcccttt cgagccgtcc gtcgcgcagc cccgtctcga ctttctgtgc agcctcggcg
    11401 aggccgctca tagagagcgt ggtcgagatc agggcagtcc ctatcatcca cctgggtttg
    11461 cagggcacga agccgaggat cttccgcgtg gagacgaaga ccagcgaagg cgttgagacg
    11521 gcgagcacga tgccagcgag gcgcctggat ctcgggctct ctaatctcag cgccctcttc
    11581 tcgaacgccg agatcacaag ccccatcaag acggtcgggt gtatggtttc tggaggttcg
    11641 ccgagcacgc cgtcgaggag cagcgccgca gcgacctgtc cccctctttg cgggctcatt
    11701 ctctgcgggg cgcccctcgg ggcttctcat tgtccgagag agcaggacgg ccgcggagcg
    11761 ccccgaccag cccgccagcc gttccgagga ggacggcacg caggacgaag ttggccatag
    11821 accgaccgag atcccgcaga ccgggtggtt gcagggtcgc ctcgacttct tcaacctgcg
    11881 ccggtggcgg gagatccctg accttctccg gggaggcccc gcgcgcttta cgctcctgcc
    11941 tgatctccat acgtacctcg tttacgtagg agacgatctt gggggctgcc tcacgcgcgt
    12001 agtcgcgggc ggcccctgcg agcagcgtcg cctgcaaagc ggtcgtcaga aacagtgtga
    12061 tccccaccat gacggcgacc accgtagccg ccagggcgcc gtcgcgtagc agcagtgatc
    12121 tgtagggcac cccgtggtcg aggcgcgtgg ggcgtaccct gagggccgcc agtatggcca
    12181 agaacaggaa cagagcaagg gccagccgcg tcctgtactg cgctatggcg gcaaggaccc
    12241 aaccaggcgt atggacgggg tcgacgaaga ggaaatacag gtctgaggcg agcgcgacga
    12301 gcacacccgc gagcccgtag aagtaacccg tcctgatcgg tcccgaaact ctcctcggct
    12361 gtccgtacat acagggagga tacagcatcc cccgcgatgg gccctgtgat cgaagtaccc
    12421 ccacgcttct caagcggtac gcgatcggcc atcagctttc agctttgtct tggctgaatg
    12481 ccaaccgccg atggctgata gctcctgaag tgtgaggcca atgccttatg cacggc
//
LOCUS       DASTKM010000045        19033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_712676, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000045 DASTKM010000000
VERSION     DASTKM010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 19033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19033
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712676"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="VFQ10_01780"
     CDS             <1..1256
                     /locus_tag="VFQ10_01780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET9964099.1"
                     /translation="LADMRLEKPMRRLLQGDVGSGKTVVAIAALLTAVEAGGQGAIMA
                     PTEVLAWQHHLSISAALADLPVNVVILTGSQGVAQRRKALEQIAAGEAHVVVGTHALI
                     QKGVVFDDLSLVVVDEQHRFGVGQRTEFREKGSTPDTLVMTATPIPRTLSLTLYGDLE
                     VSLIDELPPGRKPVETRVVDTAERLGAYEAVRKEIEEGRQAYVICPLVEESEALEDVR
                     AAEELYEELANDIFPERVVGLLHGRMKPQDKREVMALFRAGEIEVLVATVVVEVGVDV
                     PNASAIVIEGAERFGLSQLHQLRGRVCRGLHPPKCFLVGVPRSEDSERRLRALEQYQD
                     GFKLSEVDLAIRGEGTLFGSRQSGMPDLKVAKLLRDVEVLVEARREAFDLVASDPTLK
                     RPDHRPLRREIRELLGTDVEWLFRE"
     gene            1260..2306
                     /gene="coaD"
                     /locus_tag="VFQ10_01785"
     CDS             1260..2306
                     /gene="coaD"
                     /locus_tag="VFQ10_01785"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET9964100.1"
                     /translation="MRVIAGSAGGTRLAPVPRGLRPTSDRVRESLFNALGQFFEGGRV
                     LDLYAGTGALGIEALSRGIGYAVFVERDRAARAVIFENLRRTGFAQRAEVIGGEVEAV
                     VGRLAGRGELFDLIFADPPYRIAAEVGGILHRLRSLLAPGGRVAIESGEAPVEGAGGQ
                     KGVTRRYGGTFITILDRSELTMIVAVCPGSFDPVTTGHLDIIRRASKIFDHVVVAVGS
                     NRRKQARLPAVDRARLIEKVTADMESVSVEVMEGLLVEFARERGAQAIVKGLRAVSDF
                     ESEFEQAQLNRRMFPELETVFIMASAEHSFLSSSAVREIASLGGEVRGLVPEGVLETV
                     KQIYSGRDGKIQGS"
     gene            2328..2783
                     /locus_tag="VFQ10_01790"
     CDS             2328..2783
                     /locus_tag="VFQ10_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HET9964101.1"
                     /translation="MDVLVLIDRLEELVEDARSFPGFGNTAMVDRDAAFDIIDQMRQT
                     IPEELKQARWIVKERQAMLDEARSESDRIIRSAQDEAEKITSEEEVLKRAEKRSAEIM
                     EDARRREREIRLGAEDYADEVLANLEDNLGKLLEAVQRGRTKLQGVQEQ"
     gene            2803..3261
                     /locus_tag="VFQ10_01795"
     CDS             2803..3261
                     /locus_tag="VFQ10_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET9964102.1"
                     /translation="MNHLIPLRRPGAEVGDTHEVRAEFSAGPFDLYGRHHEVKKAQAD
                     VGVTRLSEGLHLDVQVVATVLTTCDRTLEPTELELEFSDSEFLSGPNNPEVCVQDWTL
                     DLTRYAERALPNEVPMQVFSPGTEPVHPEGDGDEVDPRWRGLDGLFASRS"
     gene            3288..3470
                     /gene="rpmF"
                     /locus_tag="VFQ10_01800"
     CDS             3288..3470
                     /gene="rpmF"
                     /locus_tag="VFQ10_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET9964103.1"
                     /translation="MAVPKKKTSRARRNQRRAHHAITGPRLVACPNCGAPHVGHRVCP
                     ECGFYKGRTVVAVEDI"
     gene            3490..4503
                     /gene="plsX"
                     /locus_tag="VFQ10_01805"
     CDS             3490..4503
                     /gene="plsX"
                     /locus_tag="VFQ10_01805"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET9964104.1"
                     /translation="MRIAVDALGGDNAPREIVTGALSAARGLLEDELLLVGKPGAIEP
                     EVGPDPPPNVSLRSSGEAVGMDVEPSATLRAHPDASVAVAAGMVRDGEAEAFFSAGNT
                     GATVAAALLRIGRLEGCRRPAIATLLPFPSPVLLLDAGATVSCRPQDLLNFAILGGVL
                     AKRYFRLDGEARVGLLNVGEEPGKGNDLAKEAHRILDESGIRFVGNVEGRDIPSGVAD
                     VLVTDGFTGNIVLKTAEGVAREILGMVRSAMTGDVVSKLAAGILRPRLMGVRDTVDPE
                     NYGGSFLLGVRGTVVIGHGNSQAAGVENALLGISHTGAGLTEELQQALFEHSPGLSED
                     RSA"
     gene            4500..5447
                     /locus_tag="VFQ10_01810"
     CDS             4500..5447
                     /locus_tag="VFQ10_01810"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET9964105.1"
                     /translation="MNGGAAGKIVGVGRALGNRIVTNSDLESVLDTTDEWISTRTGIK
                     ARHFVAEGENAATLAVDASKQALQHAGVDGGSVDLIICGTSTAPESMPSVACLVGEEI
                     GAPGAGAIDLAAACAGFSYAASVASSMLRSGAAGRILLIGSDAMSTIVDPKERSTAVL
                     FGDGAGAVVLDAGEGDSGFVDHILGADGRGATLGRAGHPGDDEKLFQNGREVFKFAVR
                     ILPDMVEKLLARNALSPDEVQYVIPHQANARIIRSAARKMEIPEDRVVINLDRFGNTS
                     AASIPLSYPDIYDAMEPGKYIITVGFGFGLTWAANLYRI"
     gene            5449..6276
                     /locus_tag="VFQ10_01815"
     CDS             5449..6276
                     /locus_tag="VFQ10_01815"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET9964106.1"
                     /translation="MRGDGIERAVVFPGQGTVGGEATGAARDAAVVYVGDEAVPYQLS
                     VFAGSVDLLYRLREAGIEPDVVAGHSLGEYAAAHAAGSLGLADGVRLVAERDRLMSEA
                     SRHNPGGMVALIGAEPASVESAVEEADGIVVAANFNTPRQTVVSGQQDALEEVAERVR
                     GRKVKLEVYGAFHSPLMAEAARKMDELLGEVVLEEPEIPMVGATGGALLTTAEEVREA
                     LRNQMLSPVRWVDVVERLLDLGVREIFEAGEDGTLTRLLRDFKRKDVVGRTAGEVLP"
     gene            6273..6722
                     /gene="fabZ"
                     /locus_tag="VFQ10_01820"
     CDS             6273..6722
                     /gene="fabZ"
                     /locus_tag="VFQ10_01820"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET9964107.1"
                     /translation="MKAPLGPAQIRELIPHRYPFLLVDRIEELEAGVRAAGVKNVTQN
                     EPFFQGHFPDYPVMPGVLIIEAMAQVGAVGVMAGGEHGDKLALFAGIDGVRFRRQVLP
                     GDVLRMEVEIVRLKGRVGRGKGRATVGDERVCEADLMFAFAERGEIR"
     gene            6719..7465
                     /gene="fabG"
                     /locus_tag="VFQ10_01825"
     CDS             6719..7465
                     /gene="fabG"
                     /locus_tag="VFQ10_01825"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET9964108.1"
                     /translation="MIEDGRVAVVTGGGRGIGRAVAVRLANEGANVAITYRSNDASAE
                     EVAEEVRAAGALCEIFKGDVASPEDVDALFKGVGEALGQVEILVNNAGLTRDNLMMRM
                     KEREFDEVLRTNLKGTYLCTRAALRPMLRARWGRIVNVSSVVGLVGNAGQANYAASKA
                     GIIGFTKSVAREVAQRAITVNVVAPGYVETELTRNLTEEVKNQIRGQVPAGRFAEAEE
                     VAEVVAFLAGEGAGYVTGQTVAVDGGMTMQ"
     gene            7543..7788
                     /gene="acpP"
                     /locus_tag="VFQ10_01830"
     CDS             7543..7788
                     /gene="acpP"
                     /locus_tag="VFQ10_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9964109.1"
                     /translation="MDREEILGKIQEITADRLGVDEGDVTSDASFREDLEADSLDLVE
                     LIMELEEQFGMEIPDEEAEKITTVEEAVDYVMEHQAA"
     gene            7785..9035
                     /gene="fabF"
                     /locus_tag="VFQ10_01835"
     CDS             7785..9035
                     /gene="fabF"
                     /locus_tag="VFQ10_01835"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9964110.1"
                     /translation="MIDPDASGRALVTGTGAVTPLGTGVENFWDAALHGKSGIGEITL
                     FDPSPYPSRIAGECWDFDPTDFINKKQARRMDRYAQLGIAAGIQAAESAGIVDLIRER
                     PERVAIVMGSGIGGLSTWEREYRVMNERGPGRVSPFLITMMVPNMAAGQLAIALGATG
                     PSQCPVLACATGGEAIAEGLELIRRKDADVVIAGSSEAPITPLSMAGFCAIRAVSRRN
                     DDPARASRPFDEGRDGFVMSEGAGALVLESEEHAERRGAEPISTVAGYGRTTDAYHVT
                     APDEDGRGVERAMVLALQDSGLDAEEVGHVNAHATATPTGDGPETKAIARVVPHALVS
                     GTKSMTGHSFGAVGATEGIMCALAVKHKVVPPTINLENLAEDCEELNYVGSEPIEAPD
                     LKAAISNSMGFGGHNVVIAFTSVE"
     gene            9044..9703
                     /locus_tag="VFQ10_01840"
     CDS             9044..9703
                     /locus_tag="VFQ10_01840"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HET9964111.1"
                     /translation="MSIRDLIAILPDPLRQRSLTHPSVADPYESNGRLEFLGDSVLNS
                     RVAELVFHGYRELLEGDLTRIRAHLVRKSFLAMVGRSEGIEEIIESSVINDSVIADTV
                     EAIIGGAYLIDRDLVLRTLDEIFNPAEVDTDELRDWKTLLQETLQADGLRPTYRVISK
                     QGPAHRPSFTSRVSVEGKEVATGEGSSIKESEQSAARAALEILGIRPVPRSTVEVHVG
                     A"
     gene            9844..13137
                     /gene="smc"
                     /locus_tag="VFQ10_01845"
     CDS             9844..13137
                     /gene="smc"
                     /locus_tag="VFQ10_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET9964112.1"
                     /translation="MLSAIYIKGFKTFARPVRMPLEGGITSIVGPNGSGKSNITDAVL
                     FAIGEQSPGVLRAGAMSELIFAGSETLSGASAAEVTLVLDNEAGRISLPYGEVSITRR
                     ISRSGETEYRINGSRARLADVRAVAGEAGLGRHSILRQGAVDAIVAGGPAACRLALEE
                     AAGLGVYRRRRVSASRRLERADVRLERSRQLEAELEGQLRRIQREAAAARAYREIESR
                     YRELSLAHLYRVATRELNGLRGKLDEARRQAAEMAARERDVREEETELDSRLQQLEDG
                     IGKTEQRLEALEDGAEDLRVEALRADRTLLRLEAGRGGEGETKLTVSRLEDELRRVSQ
                     TLAGLEDRARDLEAESSARREESDRLQRVVARSSEKYASAESERDRLARDLDGLRTRL
                     AGLGSQSEQRVMTEEGLSHLAAVISDLEESEDRATMENLRRLRDQVDGMLARVDELAE
                     EISRRRGALAAAVGRAESRIRALRQTAPDGASTRLYQVIRARPGYEVAVEAALGEYGA
                     GVLAENLDEGMKLVSDVEPVAVRLDARAMEPNGALPGKPLIECVEVLEARYDEAVRRL
                     LTGIFVVERPDDGANANGYVAVTRDGFRLTRTGVSLRPGPGNFARETRLSAWMEHLEA
                     LKGVPGGTLYDLKETVSGVSNRLERLAGTVDRVKVLADRTARTRALLSREARRRKTAG
                     ENARRSFLERERTISMLSDDTSRTERALAEAETDADRGREELAAATAAAEAARGAAAD
                     SERGLSEVRDTLASGASRRKAISRRLERSENALQDGAGNPEVLAARAARAATKLVTTV
                     RRRRNALRRLRGETAEAHRRVSAERTTISRKTVELAGKLATTRAQAERLAEEVCRAET
                     AAAEAGEEIQAEWGTTLDAARHEAEKHGESTDGERITLARRLKRFGDVNLLALSQEEG
                     LRERHQFISAQRADAEAAANELNRIIQSVDREIEVRFSETFRRVKEAFGEMVPRMLEG
                     SSGVLELSEDGVEIGLRLGRKGWRPLRVLSGGERALLALSFLFSVFLSRTGEDSGAFC
                     VLDEAEAALDDLNLARFLSVVDSHRANGQYLLVTHQKRTMAAADVLYGVIQDASGATT
                     VVSKRLQGE"
     gene            13199..14377
                     /gene="ftsY"
                     /locus_tag="VFQ10_01850"
     CDS             13199..14377
                     /gene="ftsY"
                     /locus_tag="VFQ10_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET9964113.1"
                     /translation="MNGPTVASESGHSTEDVERGNGHLVADLEAFEEEAAEEVPSAEE
                     VQAQRKQPAPEDVWEVEETEPGEAEDSMLESGAVEEAPETIAERQESGGWFGRLRQGL
                     SRSRTSFVGQLNAAVAEFRDVEDEEFWERVEEILISSDVGVPTTAKLVAELEQEALER
                     NITSGAELRELMIEHATRMLDGPVELDVSHMPTVILMVGVNGTGKTTTIGKLSRFLPG
                     TKMFAAGDTFRAAAIEQLQQWGERTGTPVVARERGADAAAVAHEAVHAARSEGTDVLL
                     IDTAGRLHTKVNLMAELDKVQRVVRRQLPGAPHEVLMTVDATTGQNGLRQAQMFKEVA
                     GLTGIILTKLDGTAKGGIALAIANEVGVPIKLISVGEKVEDLHPFDAREFAEALFGDL
                     "
     gene            14378..15709
                     /gene="ffh"
                     /locus_tag="VFQ10_01855"
     CDS             14378..15709
                     /gene="ffh"
                     /locus_tag="VFQ10_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET9964114.1"
                     /translation="MFDTLGERLNKALDGVRGSGNLTEDQVKKVTREIRLALLEADVN
                     YSVVKEFVSNVRERATGAEVSKALSPGQQVVKIVDDELANLMGGTAHKLSFASRPPTV
                     VMLAGLNGHGKTTAAGKLALFVRKLGKNPYLVACDTYRPAAIEQLQQIGRELSVPVYT
                     EGTGPKPEEIAERGIREARDGGYDFVIVDTAGRQVVDVEMMEEIRRVRKASRPHSVLL
                     VLDVVTGQNAVEVAQAFQEYADFDGMVLTKLDGDARGGVALSVVSVTGRPVKFASEGE
                     KMGEFDYFYPDRMAGRILGMGDVLTLIEKAEQQMDRTEAEAQGKKLMSGKFTFEDFLQ
                     QMQMIKKMGPLSGLLGMIPGLGKQLKDVEIDDRQLGRVEAMITSMTVKERRNPKLLQN
                     PSRARRIARGSGATQQDVQKLVKQFKEMQKMMAQMGKGGGMPRIPGLSGRR"
     gene            15728..15970
                     /gene="rpsP"
                     /locus_tag="VFQ10_01860"
     CDS             15728..15970
                     /gene="rpsP"
                     /locus_tag="VFQ10_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET9964115.1"
                     /translation="MAVKIRLARHGSKGNPFYRIVVADSRSPRDGRFIERIGTYDPQQ
                     EPSDIKVDEEKAREWLGKGAQPTDQVRNLLKISGVI"
     gene            15977..16216
                     /locus_tag="VFQ10_01865"
     CDS             15977..16216
                     /locus_tag="VFQ10_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET9964116.1"
                     /translation="MAQLENLLLVLARSLVDEPERVEVSGTESDSRVDLELRVAPDDM
                     GRVIGRQGRTIRAIRTVVKAASVKAGKRVTVEVPD"
     gene            16222..16716
                     /gene="rimM"
                     /locus_tag="VFQ10_01870"
     CDS             16222..16716
                     /gene="rimM"
                     /locus_tag="VFQ10_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET9964117.1"
                     /translation="MDELSDPVVIGVVVAPHGVRGSLRVRALGPGRHLREGTEPVVAG
                     MRRRISAARQTSKGFLLDLEGVKSRAEAKALGGEELLLDRKELDVPEEGEYYVTDLVG
                     LTAVNDAGEVVGKVMDTFETAAHEVLVVREDDRLDLYLPFTLEHVPEVDLRAGQVLIR
                     PPDG"
     gene            16720..17412
                     /gene="trmD"
                     /locus_tag="VFQ10_01875"
     CDS             16720..17412
                     /gene="trmD"
                     /locus_tag="VFQ10_01875"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET9964118.1"
                     /translation="MRSIDVFCVFPKAVDAAVGVGVVGRAIENEIVGYRSFDLRDYSP
                     DGRIDDMPYGGGAGMVVRVDVVARALEEVYGVSAREVRTQRRVVLTEPAGRRLTQGYV
                     EEVASGPDLTVICGRYGGIDERVWSGLATEALSLGDFVLSGGEIVAAALADATIRRLH
                     GVLGNSESLIGESFSAEDVVGPPTYTRPASWDGEEVPGVLLSGDHAKIRAWREHAALE
                     RTRARTTGRRSP"
     gene            17446..17772
                     /gene="rplS"
                     /locus_tag="VFQ10_01880"
     CDS             17446..17772
                     /gene="rplS"
                     /locus_tag="VFQ10_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET9964119.1"
                     /translation="MITSDNMQQRDVTFKPGDTVRVHYRVIEGNRERVQVFEGLCISR
                     KGSGIHETFTVRKNSFGVNVERIFPLHSPKIAKIEVASRGAVRRAKLYYIRDKVGKKA
                     RVKKAR"
     gene            17786..18412
                     /locus_tag="VFQ10_01885"
     CDS             17786..18412
                     /locus_tag="VFQ10_01885"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET9964120.1"
                     /translation="MSGTRSTGLPPEESSLYAFDAAWVLHRRAPLAGADEAGRGALAG
                     PLVAAAVVLGDAEIFGINDSKLLRPTLREEIFGALLRRAEAVSVVAYPAWWIDRHGVG
                     LANREVLSRALASLKSRSGCGLADGNLDLGPEVECLPRADARSAAVAAASVVAKVLRD
                     GAMRALSETHTGYGFGRNRGYGTPEHRLALSELGPCRVHRLSYAGVGD"
     gene            complement(18430..>19033)
                     /locus_tag="VFQ10_01890"
     CDS             complement(18430..>19033)
                     /locus_tag="VFQ10_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964121.1"
                     /translation="WHLISGLLKDPERLKAGLEEMIEQERAGLRGDPDQEAKAWMERL
                     SEVDQERRGYLRLAAKGVLTDEELNEALAELENFRETAEKELRAITTRREVLENLERD
                     RDALLESYASMAPAALDGLSSEERHQIYKMLRLTVEISPDGSLDVSGVLRDSFVSENQ
                     DEGIVLAHYQVDLPSPRSVVALHERVSVLDQVAQGEVFTP"
BASE COUNT         3733 a         5534 c         6561 g         3205 t
ORIGIN      
        1 tcctcgccga catgcgcctg gagaagccga tgcgccgcct ccttcagggt gacgtcggca
       61 gcggaaagac cgtggtcgcc atcgccgcgc tcctcaccgc ggtcgaggcg gggggacagg
      121 gtgccataat ggctccgacc gaggtccttg cctggcagca ccacctctcg atctcagctg
      181 ccctggccga cttgcctgtc aacgtcgtaa tcctcaccgg ctcccagggt gtggcgcaga
      241 ggcgtaaggc actggagcag atagccgcag gcgaggccca cgtcgtcgtc gggacgcacg
      301 ccctcataca gaaaggcgta gttttcgacg acctctcgct cgtagtcgtg gacgagcagc
      361 accgcttcgg cgtcggccag cggacggagt tccgcgagaa agggtccacc ccggacaccc
      421 tcgtcatgac ggccacccct ataccaagga ccctctccct cacactctac ggcgacctcg
      481 aggtctccct gatagacgaa ttgccgccgg gaaggaagcc ggtcgagacc agggtcgtcg
      541 acaccgcgga acggctaggt gcctacgagg cggtcagaaa ggagatcgag gaagggcggc
      601 aggcttacgt gatatgcccg ctcgtcgagg agtctgaggc cctggaagac gtacgcgccg
      661 ccgaggagct ctacgaggag ctcgcgaacg acatcttccc cgagagagtg gtcgggctgt
      721 tgcacggcag gatgaagccc caggacaagc gcgaggtgat ggccctgttc cgggcagggg
      781 agatcgaggt gcttgtggct accgttgtcg tggaggtcgg tgtcgacgtg ccaaacgcga
      841 gcgcgatcgt aatcgaaggc gctgagcgtt tcgggctctc ccaactccac cagctgaggg
      901 gccgcgtgtg cagaggcctg catcccccga agtgtttcct cgtaggagtc cccaggtccg
      961 aagattccga acgacgtctc agggctctgg agcagtatca ggacgggttc aagctctccg
     1021 aagtcgacct ggccataagg ggcgagggga cccttttcgg tagtcgccag agcggcatgc
     1081 ctgacctcaa ggtcgccaag ctcttgcgcg acgttgaagt tctcgtcgag gctagacgcg
     1141 aggcgttcga tctcgtggct tctgacccca cgctgaagcg gcccgaccac aggcccctgc
     1201 ggcgagagat ccgtgagttg ctcggcacgg acgtcgagtg gctcttcagg gaataggtct
     1261 tgagggttat cgccgggtcg gccggaggca ccaggctggc acccgtcccc aggggcttgc
     1321 gccccacgtc cgaccgcgtg cgggagagcc tattcaacgc cttgggacag ttcttcgaag
     1381 gcggcagggt cctcgacctc tacgcgggga ctggcgcgct cgggatagaa gccctgagcc
     1441 gagggatcgg gtacgccgtc ttcgtcgagc gggatcgggc ggccagggct gtgatcttcg
     1501 agaacttaag gaggaccggc ttcgcacaga gggcggaggt aatcgggggc gaggtcgagg
     1561 ccgtggttgg gcggctcgca gggcgcggtg aactgttcga tttgatcttc gccgacccac
     1621 catatagaat cgcagcggag gtcgggggaa tcttgcaccg gctacgctca ttgctcgcgc
     1681 cgggaggaag ggtcgcgatc gagagcggcg aggctccggt tgagggcgca ggaggccaga
     1741 aaggggtaac gcgccgctac ggcggcacgt tcataaccat acttgatcgg tccgagctga
     1801 caatgatcgt ggctgtgtgt ccaggtagct tcgaccctgt aacgacgggg cacctcgaca
     1861 tcatcaggcg cgcttccaag atcttcgacc acgtggtcgt cgccgttggt agtaaccgcc
     1921 gcaagcaggc ccgcctcccc gcggtcgacc gggcgaggct aatagagaag gtgaccgccg
     1981 acatggagag tgtctccgtc gaagtgatgg aaggtctcct cgtggaattc gccagagaac
     2041 gaggggcgca ggcgatcgtg aagggcttgc gggccgtctc cgacttcgag tcggagttcg
     2101 agcaggcgca gctgaaccgg aggatgttcc ccgagctcga aacagtcttc ataatggcct
     2161 cggcggagca cagtttcctc tcctcgagcg cggtcaggga gatagcatcg ctcggtgggg
     2221 aggtgcgcgg gctcgtacca gaaggcgtgc tcgagaccgt aaagcagata tattctggtc
     2281 gggacggtaa gatacaggga tcgtaggcga aggataggta gtagaagatg gacgtactgg
     2341 tactcataga cagattggaa gaactggtcg aggatgctcg cagcttcccc ggtttcggca
     2401 acacggcgat ggtcgaccgg gacgccgcct tcgatatcat agaccagatg cgccagacca
     2461 taccggagga gctcaagcag gcccgctgga tcgtcaagga gcgccaggcg atgctcgacg
     2521 aggcccgcag cgagagcgac cgcatcatcc gcagcgccca ggacgaggcc gagaaaatca
     2581 cttcggaaga ggaggtcctc aagagggcgg agaagcgtag cgcggagatc atggaggacg
     2641 cccgccgccg cgagcgcgag atacgcctcg gcgccgagga ctacgccgac gaggtcctcg
     2701 ccaacctcga ggacaacctg ggcaaactcc tcgaggccgt gcagcggggg cgcacaaagc
     2761 tgcagggcgt ccaggaacaa tagcaagtag taaactaacc tcatgaacca tttgatcccc
     2821 ctcaggcgac ccggagcgga ggtcggagac acccacgaag tgcgtgccga gttttccgca
     2881 ggtcctttcg acctctacgg gcgccaccac gaggtaaaga aggcacaagc agatgtaggc
     2941 gtcacgcgtc tctccgaggg cctgcacctc gacgtgcagg tcgtcgcgac ggtgcttacg
     3001 acctgcgaca ggacgctcga gcccacggag ctcgaactgg agttcagcga ctccgagttc
     3061 ctctcggggc cgaacaaccc tgaggtctgt gtccaggact ggaccctgga tctgacgcgc
     3121 tatgcggagc gcgccctgcc gaacgaggtc ccgatgcagg tgttctcgcc cggcaccgag
     3181 ccagtgcacc ccgaaggtga tggcgacgag gtagacccgc gctggcgggg cctcgacggc
     3241 ctcttcgcct cgcgatcttg agcctatagc aaccaggagg tattcgcatg gccgtaccca
     3301 agaagaagac gtcacgggcg cgcaggaacc agcgccgggc gcaccacgca atcacaggcc
     3361 cgcgtctggt cgcctgcccc aactgcggcg cgccgcacgt ggggcatagg gtctgccctg
     3421 agtgcggttt ctacaaaggc cgcacggtag tcgctgtgga ggacatctag accccgataa
     3481 ggccgtccag tgcgtatcgc ggtagacgcc ctcggcggag acaacgcccc gcgggagata
     3541 gtcaccggcg ccctatccgc tgccagaggc ttgttggaag acgagttgtt gctcgtcggg
     3601 aaacctggtg ccatcgaacc tgaggtgggc cctgatcccc ctccgaacgt ctcgctgcgg
     3661 tcctcagggg aagccgtcgg gatggacgtg gagccctctg ctacgctcag ggcccatccc
     3721 gacgccagcg tcgccgttgc ggccggcatg gttcgtgacg gggaggccga ggcgttcttt
     3781 tcggcgggga acaccggcgc cacagtggcg gcggcgttgc taaggatagg acgcctcgaa
     3841 ggatgccgca ggcccgcgat cgccactttg ctacctttcc cttcacccgt cttgctactc
     3901 gacgctgggg ctaccgtctc ctgcagaccg caggacctct tgaatttcgc tattcttggc
     3961 ggtgtgctcg cgaaacgtta cttcaggttg gacggagaag cgcgggtcgg actgctcaat
     4021 gtcggcgagg agccggggaa gggcaatgac ctcgccaaag aggcgcaccg tatcttggac
     4081 gagtctggca ttcgtttcgt cgggaacgtg gagggtcgcg acatccctag cggggttgct
     4141 gacgttctcg taacggacgg ctttacgggt aacatcgtcc tcaagacggc cgagggggtg
     4201 gcgcgtgaga tcctcggcat ggtccgctcc gcgatgacgg gcgatgttgt gagcaaactc
     4261 gccgcaggta tccttcgtcc gcggctgatg ggggtgagag acacggtgga tcctgagaac
     4321 tacggtggtt ccttcctgct cggcgtaagg ggcacggtcg tgatcggcca cggaaactcg
     4381 caagctgctg gcgtcgagaa cgcgttactc ggtatctcgc acacgggcgc cggcctgacc
     4441 gaggaactgc agcaggcgct ctttgagcac agtcctggtc tgtctgagga caggtcggct
     4501 tgaacggcgg tgctgccggc aagatcgtcg gtgtcggaag ggctctcggg aacaggatcg
     4561 tcaccaacag cgacctcgaa agcgtgcttg acaccacgga cgagtggatc tcgacgcgta
     4621 ccgggataaa agctcgccac ttcgtcgctg aaggcgagaa tgccgcgaca ctcgccgtag
     4681 atgcctcgaa gcaggccctc cagcatgccg gggtcgatgg cgggtctgtc gacctcatca
     4741 tctgcggtac ttccacggcg ccggaatcca tgccttcggt ggcctgcttg gtgggagagg
     4801 agataggggc tccaggcgca ggcgctattg accttgcggc agcgtgtgcc ggcttctctt
     4861 atgctgcctc ggtcgcttca tccatgctcc gctcgggcgc ggccggcagg atcttgctca
     4921 tcgggtcgga cgcaatgagc accatcgtcg accccaagga gcgctcgacg gccgtccttt
     4981 tcggagacgg cgcaggcgca gtcgtactgg atgccggcga aggggactcg ggtttcgtcg
     5041 accacatcct cggggccgac ggcagggggg ctacgctggg gcgcgccggc cacccgggag
     5101 atgacgagaa gctcttccag aacggccgag aggtcttcaa gtttgccgtc aggatactgc
     5161 ccgacatggt cgagaagctc ctcgcccgca acgcactctc gccggacgag gtccagtacg
     5221 taatacccca ccaggcaaat gcacgcataa tacgatcggc ggcacggaag atggagatac
     5281 ccgaggatag ggtcgtgatc aacctcgacc ggttcgggaa tacttcggcg gcgagcatcc
     5341 cactctccta ccctgacatc tacgacgcga tggagccggg aaagtacatt atcaccgttg
     5401 gcttcgggtt cggcttgacc tgggctgcga acctgtacag gatctagttt gcggggtgac
     5461 gggatagagc gtgccgtggt gttccccggg cagggtaccg ttggagggga ggccaccggc
     5521 gccgcgcgcg acgccgcggt tgtctatgtc ggggatgaag ctgtgcctta ccagctctct
     5581 gtattcgccg gctccgttga tcttctctac cgtttgcggg aggcagggat cgagccagac
     5641 gtggtggccg gccactcgct cggcgagtac gcggcggccc acgctgcggg gagcctaggt
     5701 ctcgccgatg gtgtgcggct cgtggcggag agggatcgcc tgatgagcga ggcctcgagg
     5761 cataatcctg gagggatggt agccctgatc ggggccgagc ctgctagtgt cgagagcgct
     5821 gtcgaagagg ccgacggtat agtcgttgcg gcgaacttca acacgccgcg ccagaccgtc
     5881 gtttccgggc agcaggatgc gctcgaggag gtcgctgagc gtgtacgtgg gcggaaggtg
     5941 aagctggagg tatacggggc tttccattcg ccgctcatgg cagaggctgc gcgaaagatg
     6001 gacgagcttc tcggcgaagt cgtcctggaa gaacccgaga taccgatggt cggggcgacg
     6061 ggtggagcgt tgttgacgac ggcggaggag gtgcgtgagg cattgcggaa ccagatgctc
     6121 tcccccgtgc ggtgggtgga cgtggtagaa cgcttgctgg atcttggagt gcgtgagatc
     6181 ttcgaggccg gggaggatgg gaccctgaca aggttgctgc gagacttcaa gagaaaagac
     6241 gtagtgggac ggacggccgg ggaggtgctc ccgtgaaggc accactcggg ccggcgcaga
     6301 tccgggaact cataccccac cgctacccct tcctcctagt ggacaggatc gaggagctcg
     6361 aagccggtgt gcgggccgcc ggcgtaaaga acgtcaccca gaacgagcct ttcttccagg
     6421 gtcactttcc tgattatcct gtcatgcccg gtgtgctcat tatcgaggcg atggcccagg
     6481 taggcgctgt cggcgtcatg gccggcgggg agcacggtga caagctcgcg ctcttcgcgg
     6541 ggatagacgg cgtaaggttc cgcaggcagg tcctgcctgg cgacgtgctc aggatggagg
     6601 tcgagatcgt ccgtctcaaa ggcagggtag gaaggggtaa gggacgggct acggtcgggg
     6661 acgagcgggt ttgcgaggct gacctgatgt tcgccttcgc ggagcgaggg gagatccggt
     6721 gatcgaggac ggacgcgtcg ccgtcgtcac gggcggagga aggggaattg gccgcgccgt
     6781 ggcagtgcga ctcgcgaacg aaggcgcgaa cgtagcgatc acttaccgat ccaacgacgc
     6841 gagcgcggag gaggtggcag aggaagtaag ggccgcgggg gcgttgtgcg agatcttcaa
     6901 gggtgacgtt gcctctcctg aggacgtcga tgcgctgttc aaaggcgtgg gcgaagccct
     6961 cggacaggtc gagatactcg tcaacaacgc tggcctgacc agggacaatc tgatgatgcg
     7021 catgaaagag cgtgagttcg acgaggtgtt gcggaccaac ctcaagggca cctacctgtg
     7081 cacgcgggcg gctctgaggc cgatgttaag ggccagatgg gggcgaatcg tcaacgtgtc
     7141 ctctgtcgta gggcttgtcg ggaacgcggg gcaggccaac tatgctgcct ccaaggccgg
     7201 gatcatcggg tttaccaaga gcgtggcacg ggaggtggcg cagcgcgcca ttacggtcaa
     7261 cgtcgtggcg cctgggtatg tcgagaccga gctgacgcgt aacctgaccg aggaggtcaa
     7321 gaaccagatc aggggccagg tacctgcggg caggttcgca gaggcggagg aggttgctga
     7381 ggtcgttgcg ttccttgctg gcgaaggcgc cggatacgtt acaggccaga ccgtggccgt
     7441 cgacggcggc atgactatgc agtaaggcgt tttaggttta cactacgcgc tatcgttgga
     7501 cctgtcatag taatttgtaa gagtcatcga gaggagagac gcttggatcg cgaagagata
     7561 ctcggcaaga tacaggagat caccgccgac aggctgggag tagatgaggg cgacgtcact
     7621 tccgacgctt ccttccgcga ggatttggag gctgattccc tggatctggt cgagctcatc
     7681 atggagctcg aggagcagtt cgggatggag atccccgacg aagaggccga gaagatcacc
     7741 acagtcgagg aggccgttga ctacgtgatg gagcatcagg ccgcgtgatc gaccccgacg
     7801 cgagtgggag ggcgctcgta accggaaccg gggctgttac accccttgga acaggggtcg
     7861 agaacttctg ggatgcagcc ctgcacggta agagcggaat aggggagatc acgctcttcg
     7921 atccgtcccc ttatccttcc cggatagccg gtgaatgctg ggatttcgat ccgacggact
     7981 ttataaacaa gaagcaggcc cgcaggatgg accgctatgc ccagctcggc atagccgcgg
     8041 gcatacaggc cgcggagagc gcggggatag tggacctgat ccgcgagagg cccgagcgcg
     8101 tggcgatcgt gatgggcagc ggtatcgggg gactctccac ctgggagcga gagtacaggg
     8161 tcatgaacga gcggggaccc ggaagggtga gtccgttcct gatcacgatg atggtcccca
     8221 acatggcggc cgggcaactc gctatcgcgc tcggcgccac gggacctagc cagtgtcctg
     8281 ttcttgcctg cgccacagga ggggaggcga tcgcggaggg gctggagctg atccggcgca
     8341 aagacgccga cgtggtgata gccggctcct cggaagcccc gataacgccc ctctctatgg
     8401 ctggcttctg tgccatacgg gccgtgagtc gccgcaacga cgaccccgcc agggcaagtc
     8461 gtcccttcga cgaaggccgc gacggtttcg tgatgagcga gggcgccggt gccctggtcc
     8521 tggagagcga ggagcacgcg gagcgccgcg gcgcagaacc gatctccacc gtcgccggct
     8581 acggccgtac gaccgacgcc taccacgtaa ccgcgcccga tgaggacggc cgtggcgtcg
     8641 agcgggcaat ggtcctcgct ctccaggatt ccggcctcga cgccgaggag gtcggccacg
     8701 tcaacgccca cgccacagcc accccgaccg gagacgggcc agagacgaag gcgatagcga
     8761 gggtcgtgcc gcacgccctg gtctctggta cgaagtcgat gacagggcac tccttcgggg
     8821 ccgtcggcgc caccgaaggc atcatgtgcg cccttgccgt aaagcacaaa gtcgtcccac
     8881 cgacgatcaa cctcgagaac ctcgcagaag actgcgaaga gctgaactac gtaggaagtg
     8941 agccgatcga ggcccctgac ctcaaagccg ccatctcgaa ctccatggga ttcggcggcc
     9001 acaatgtcgt gatcgcgttt acaagtgtag aatagagaac gagatgtcta taagagacct
     9061 catagcgatt ctaccagacc ctttgaggca gcgctcgttg acccatccga gcgtcgctga
     9121 cccttacgag tcgaacggga ggctcgaatt tctcggggac tccgttttga acagccgcgt
     9181 cgccgagctg gtcttccacg gctaccgcga gctactggag ggggatctga cgcgcatacg
     9241 ggctcatctg gtaaggaagt catttctggc aatggtaggc cggagtgagg gtatagagga
     9301 gatcatcgag agctccgtca taaacgactc ggttatcgcc gataccgtgg aggccattat
     9361 cgggggggcc tacctcatag acagggacct ggtgctcagg acgctggatg agatcttcaa
     9421 ccccgcagag gtcgataccg acgagctgcg tgactggaag acgctccttc aggaaacgct
     9481 gcaggcagac gggctcaggc cgacgtaccg ggtcatctcc aagcaaggac cggcccaccg
     9541 gccaagcttc acctccaggg tctctgtgga aggaaaggaa gtcgctacgg gtgaaggcag
     9601 ctccataaag gaaagcgagc agagcgccgc ccgagccgcg ctggagatac taggcatccg
     9661 ccccgtccca cgctccacgg tcgaggtaca cgtaggcgcc tagagctaat cagcacgctc
     9721 aggcctctaa ggccttcact tgtcatcctg ccgcccgctg gcagcgtgct gaggagctga
     9781 cagctctttt gctgcacttt tccccgtgct ttcatagcgg aagttgcgta aaatacccat
     9841 tcagtgctct cggcgatcta tataaagggc ttcaagacct tcgcccggcc cgtcaggatg
     9901 ccactcgaag ggggcatcac gtcgatcgtc ggtcccaatg gttcggggaa gagcaacatc
     9961 accgacgctg tgctcttcgc gatcggggag cagagcccgg gggtattgcg ggcaggagct
    10021 atgagcgagt tgatcttcgc cggctcggag acgctttcgg gtgcgagcgc agccgaagta
    10081 accctcgtac tcgacaacga agcgggcagg atctcgctcc cttacgggga ggtttcgatc
    10141 acgcgccgga tctcccgcag cggggagacg gaatacagga tcaacggctc ccgtgcccgt
    10201 cttgcggacg tacgcgccgt tgctggcgag gcaggactgg gacgccacag catcctacgc
    10261 cagggtgccg tggacgcgat agtcgctggt gggccggccg cgtgccggct cgcgctggaa
    10321 gaggccgcgg gactcggtgt ctacaggagg cgccgcgtct cggctagccg ccgtctggag
    10381 agggccgatg tccgactcga acgcagccgc caactggagg ccgagctcga gggccagttg
    10441 cgcaggatac aaagggaggc cgccgccgcc cgcgcgtacc gggagatcga atcccgctac
    10501 cgcgagctct ccctggctca cctctatcgg gtcgcgacca gggagttgaa cggacttagg
    10561 gggaagctcg acgaggccag gaggcaggct gcagagatgg ccgcgcgcga gcgggacgtt
    10621 cgtgaagaag agacagagtt agatagccgc ctgcaacaac tcgaggatgg gatcggaaag
    10681 accgaacagc gactcgaagc cctggaggat ggcgccgagg accttcgggt ggaggcccta
    10741 cgcgcggaca ggactctctt gcgactagag gcgggccgcg gaggagaggg ggagacgaaa
    10801 ctcaccgtgt cccgcctgga agacgagtta cgccgggtgt cgcaaacgct ggctggtctc
    10861 gaagatagag ccagagatct agaggccgag tcttcagcga ggcgcgaaga gtcggaccgg
    10921 ctccagaggg tcgttgcgag atccagcgag aagtacgcgt ccgccgaaag cgagagggat
    10981 aggctggcca gggacctgga tggacttcgc acgcgcctcg ccgggcttgg gtcccaaagt
    11041 gagcaacgcg tgatgaccga agagggcctc tcacaccttg cggccgtgat ctcggacctc
    11101 gaagaatcgg aagaccgcgc cacgatggag aatctccgca ggctgcgcga tcaggtagac
    11161 ggcatgctcg cacgggtaga tgagctcgcc gaggagatca gccgcaggcg cggggccctc
    11221 gccgctgctg ttgggagggc agaatccagg atacgtgcgt tgaggcagac tgcccctgac
    11281 ggcgcgagca cgcggctcta ccaggtgatc cgggcccgcc ccggttacga ggtcgccgtc
    11341 gaggcggccc ttggagagta cggtgccggg gtgcttgcgg agaacctgga cgaggggatg
    11401 aagcttgttt cggacgtcga gcctgtggcc gtccgcctcg acgcgagggc catggaaccc
    11461 aacggggccc tgcctggaaa acccctgata gaatgcgtag aggttctgga ggcgcgctac
    11521 gatgaagcgg tccggcgcct cctgacaggt atcttcgtcg tcgagcgtcc tgatgacggc
    11581 gcgaacgcaa acgggtacgt ggccgttacg agggatggct ttcgcctcac gaggaccggt
    11641 gtgagcctgc gtcccggccc tggcaacttc gcgcgcgaga cgcgactttc cgcctggatg
    11701 gaacacctcg aagccctgaa aggcgtgccc ggcggaaccc tctacgacct gaaggagact
    11761 gtctctggtg tctcgaatcg cctggagagg ctcgccggaa ccgtagatag ggtcaaggtt
    11821 ctcgctgacc ggacggcgcg cactcgagcc ctgttatcac gcgaagcaag acgccgcaag
    11881 accgcaggag agaatgcgag gagatccttt ctggagcggg agaggactat ctctatgctc
    11941 tccgacgaca cttccaggac ggagagagcg cttgcggaag ccgaaacgga tgcagaccgc
    12001 gggagggagg agctcgccgc tgccaccgcc gccgccgagg cggcccgcgg cgccgctgct
    12061 gactcggaac gggggctgtc ggaggtgcgc gataccctcg cctctggcgc aagtcgcagg
    12121 aaagctatct cacgcaggct ggagcggtcc gagaacgctc ttcaggacgg agcaggcaac
    12181 ccagaagtgc tcgccgccag ggcggcgagg gcggccacaa agctcgtcac gaccgtccgc
    12241 cggcgcagaa acgccttgcg gcgcctccgt ggagagactg ccgaggcgca ccgccgggtc
    12301 tcagcggagc gcacaaccat ctcgcgcaag accgtggaac tcgcaggcaa gctcgccacc
    12361 actcgcgcgc aagccgaacg cctcgccgaa gaggtgtgca gggcagaaac cgcggccgcc
    12421 gaggcaggcg aggagataca ggcggagtgg ggcaccaccc ttgatgctgc ccgccacgag
    12481 gcagagaagc acggggagag tactgacgga gaacgtatca ccctcgcccg caggctcaag
    12541 cgctttgggg acgtaaacct cctcgccctc tcccaggaag agggactgcg tgaacgtcac
    12601 cagttcatct cggcccagag ggccgacgca gaggccgcag ccaacgaact caaccgcata
    12661 atacaatccg tggacaggga gatagaggta cgtttctcgg agaccttccg gagagtaaag
    12721 gaggcgttcg gggagatggt gccgcggatg ctcgaaggat cttctggggt actggagctt
    12781 tctgaggacg gggtggagat cgggttgcgg ctggggagga agggttggag acccttgcgc
    12841 gtcctatcag ggggcgaacg ggccctactc gcgctgtcgt tcctgttcag cgtgttcttg
    12901 agccgcactg gtgaggattc tggggctttc tgcgtcctcg atgaggccga ggcggcgctc
    12961 gacgatctca acctggcccg tttcctctct gtggtagact ctcaccgggc taacggccag
    13021 tatctgttgg tcacccatca gaaacgaacg atggccgcgg cggatgtgct ctacggggtg
    13081 attcaggatg cctcaggggc cactactgtt gtgtcgaagc gtttgcaggg agagtaagaa
    13141 gtcgcctatg gcacgctcgt ggcgtagttt tttcagacga gggacagatg aagagcaggt
    13201 gaacggcccg acggtagcca gtgagtcggg gcattccacc gaagatgtcg aacgaggcaa
    13261 cggccacctc gttgctgacc tcgaagcatt cgaggaggaa gccgccgaag aggtgccttc
    13321 ggccgaggag gtgcaggccc agaggaagca gcctgcaccc gaggacgtgt gggaggtcga
    13381 agagaccgaa cccggtgagg ctgaggactc tatgctcgag tcaggcgctg tcgaggaggc
    13441 gcctgagact atcgccgagc ggcaggagtc cggcgggtgg tttggcaggc tgaggcaggg
    13501 cctcagccgt agccgcacat cgttcgtcgg acagctcaac gccgccgtcg ccgagttcag
    13561 ggatgtcgag gacgaggagt tctgggagag agtcgaggag attttgatct cctccgacgt
    13621 cggggttccg accacggcga agctcgttgc ggaactcgaa caggaggcgc tggagaggaa
    13681 catcaccagc ggtgccgagt tacgggagct catgatcgag cacgccacac gcatgctcga
    13741 tgggcccgtc gagctcgacg tctcacacat gcctactgtg atcctgatgg tcggggtgaa
    13801 cgggaccggc aagacgacga cgataggcaa gctctcgcgt ttcttgccgg ggaccaagat
    13861 gttcgccgca ggggacacgt tcagggctgc ggccatcgag cagctccagc agtggggcga
    13921 gcggacgggt acgcccgtgg tggcgcgcga gcgcggagcc gacgcggctg ccgtcgccca
    13981 tgaagccgtg catgccgcca ggagcgaggg tactgacgtg ctgctcatcg acacggcggg
    14041 caggctccac accaaagtaa acctgatggc cgagctcgac aaagtacaga gggttgtaag
    14101 gcggcagctt ccaggggcgc cgcacgaggt actgatgacg gtcgacgcga ccacgggtca
    14161 gaacgggttg cggcaggccc agatgttcaa agaggtagcc gggctcaccg ggatcattct
    14221 caccaagctc gacgggactg ccaagggcgg catcgccctc gccatagcca acgaggtggg
    14281 ggtgccgatc aagctcatct cggtcgggga gaaggtcgag gacctccacc cgtttgacgc
    14341 ccgcgagttc gcggaagccc tgtttggaga tttatagatg ttcgatacgt taggcgaaag
    14401 gctcaacaaa gccctcgacg gggttcgcgg tagcggcaac ctcaccgagg accaggtaaa
    14461 gaaggttacg cgtgagatcc ggctggccct cctcgaggcc gacgtgaact attcggtcgt
    14521 caaggagttc gtctccaacg tccgcgagcg ggccacaggc gccgaggtct cgaaggccct
    14581 cagtcctggc cagcaggtcg tcaagatagt cgacgacgag cttgcgaacc tgatgggcgg
    14641 caccgcccac aaactctcct tcgcctccag gccgcctacg gtcgtgatgc tcgcgggcct
    14701 aaacggccac ggcaagacga cggcggccgg gaagctcgcg ctgttcgtgc ggaagctagg
    14761 gaagaacccc tatctggtcg cgtgcgacac ttacaggcct gccgcgatcg agcaactgca
    14821 gcagatcgga cgcgagctct cagttcccgt gtacacggag ggcacgggtc cgaagccgga
    14881 agagatcgcc gagcgcggca tcagggaggc cagagacggc gggtatgact tcgtgatcgt
    14941 cgacacggcg ggcaggcagg tagtcgacgt ggagatgatg gaggagatcc ggcgcgtcag
    15001 gaaggcctcg aggccgcact ccgtcctact cgtgctcgac gtggtaaccg gccagaacgc
    15061 cgtcgaggtg gcgcaggcct tccaggagta cgccgacttc gacgggatgg tcttgaccaa
    15121 gctggacggt gacgcccgcg gcggggttgc gctctccgtc gtctccgtaa cggggcggcc
    15181 cgtcaagttc gcctctgagg gtgagaagat gggcgagttc gattacttct accccgacag
    15241 gatggctggt cgcatcctgg gtatgggcga tgtcctcacg ctcatcgaga aggccgaaca
    15301 gcagatggac cgtaccgagg cggaggccca gggcaagaag ctcatgagcg ggaaattcac
    15361 cttcgaggac tttctgcagc agatgcagat gataaagaag atgggtccgc tatcgggcct
    15421 cctcggcatg atcccgggcc tgggcaaaca gctcaaggac gttgagatag acgacaggca
    15481 gctcggcagg gtggaggcca tgataacctc gatgaccgtc aaggagcgca ggaaccccaa
    15541 gttgttgcag aacccgagcc gggcgcgtag gattgcgcgg ggctcgggcg cgacccagca
    15601 ggacgtccag aaactcgtca agcagttcaa ggagatgcag aagatgatgg cccagatggg
    15661 caagggcggc gggatgccgc gcataccggg tctgtcgggg cgcaggtaga gttcgagagg
    15721 agatagcatg gcggtaaaga taaggctcgc gaggcacggc tccaagggca accccttcta
    15781 caggatagtg gtcgccgatt cacggagtcc gcgcgacggc cggtttatag agcggatagg
    15841 gacctacgat ccgcagcagg agccttctga catcaaagtc gatgaagaga aggcaaggga
    15901 gtggttaggc aagggggcgc agcctactga tcaggtgcgt aacctgctga agatctccgg
    15961 ggtcatctag gtagccgtgg cgcagttaga gaaccttctg ctggtcctcg cccgctcgct
    16021 cgtggacgaa ccagagaggg tcgaggtctc agggaccgag tctgactcgc gggtggatct
    16081 ggaactgagg gtggcgccag acgacatggg gcgtgtgatc ggccgccagg gacggacgat
    16141 ccgagcgatc cgcacggtcg tgaaggccgc atccgtgaaa gcgggaaagc gggtcaccgt
    16201 ggaggtccct gactagagac catggacgaa ctctccgatc cggtcgtgat cggggtcgtg
    16261 gtcgcaccgc acggggtacg cggctccttg agggtccgtg ccctcggccc aggcaggcac
    16321 ctcagagaag gtacagaacc ggtggttgca ggcatgaggc gccgtatctc tgcagccagg
    16381 cagacctcga agggcttctt gctcgacctc gaaggcgtaa agagcagagc cgaggcaaag
    16441 gccctgggag gcgaagagct cttgctggac cgcaaggagc tcgacgtacc cgaagaagga
    16501 gagtattacg tgacggacct ggtcggcctt acggctgtga acgatgcggg tgaggtcgtc
    16561 ggaaaggtaa tggatacgtt cgagactgcc gcccacgagg ttctcgtggt ccgtgaagat
    16621 gatcgactag acctttatct cccctttacc ctcgaacatg tcccagaggt ggacctgcgg
    16681 gccgggcagg tactgatccg cccgccggat ggctagacgg tgagaagcat agacgtcttc
    16741 tgtgttttcc ccaaggcggt cgacgccgcc gtcggcgtcg gggtcgtcgg cagggccata
    16801 gagaacgaaa tcgttggcta ccgcagcttc gacctgcgtg actactcacc tgatggtcgg
    16861 atcgacgaca tgccatacgg tggcggcgcc gggatggtcg tcagggtcga cgtcgtagcg
    16921 agggcgctcg aggaggtcta cggcgtttcg gcgagggagg taagaacgca gcggcgggtc
    16981 gtactcacgg agccggccgg acgcaggctc acacagggct acgtcgagga ggtggcttca
    17041 gggccagatt tgaccgtcat ctgcggacgc tacggaggga tagacgaacg ggtttggagc
    17101 gggctcgcca ctgaagccct ctcactgggc gactttgttt tatctggggg tgagatcgtg
    17161 gccgccgcgc tcgctgatgc cacgatccgg cgtctccatg gcgtcctcgg gaactccgag
    17221 agcctcatcg gcgagtcgtt ctcagccgaa gatgttgtcg gacctcctac ctacacgcgg
    17281 ccagccagct gggatggaga ggaggtacca ggcgtgctat tatcgggcga tcacgcaaag
    17341 atccgcgcct ggcgcgagca cgcggcgctg gagcgtacgc gggcgaggac gaccggaaga
    17401 aggtcaccct agccatccga agtaggtcca cgagaggaga agaacatgat aacgagtgat
    17461 aacatgcagc agcgggacgt aacgtttaag ccgggcgata cggtgcgggt tcactaccgc
    17521 gttatcgagg gcaaccgcga gcgtgtacag gtcttcgaag gactgtgtat ctcgcgcaag
    17581 ggcagcggga ttcacgagac gttcacggtc aggaagaact cctttggggt gaacgtggag
    17641 aggatcttcc cgctccactc cccgaagatc gccaagatcg aagtcgcctc gcgtggcgcg
    17701 gttagacgcg cgaagctcta ctacatccgc gacaaggtgg gcaagaaagc cagggtcaag
    17761 aaggccaggt aagccaacac cggcgttgtc agggactcgc tctaccggcc ttccgccgga
    17821 ggagagttcc ctctacgctt tcgacgcggc atgggtcctc catcgccgcg cacctcttgc
    17881 cggggccgat gaggcgggcc gtggcgcgct ggcaggaccg ctggtggccg cggccgtcgt
    17941 ccttggcgac gctgagatct tcgggatcaa cgattccaag ttgctacggc ccactttgcg
    18001 cgaggagatc ttcggagcgc tgctccgcag ggccgaggcc gtttccgtgg tggcttaccc
    18061 agcctggtgg atcgaccgcc acggcgtcgg cctggcgaac cgagaggtcc ttagcagggc
    18121 tctcgcctcc ctgaagtccc gctccgggtg cggccttgcg gatggcaacc tggatctcgg
    18181 tcccgaggtg gagtgcctgc ccagagctga tgccaggagc gcggcggtcg ccgccgcgag
    18241 tgttgtggcc aaggtccttc gcgacggcgc gatgcgcgcc ctatcagaga cccacactgg
    18301 ctacggcttt gggcgcaatc gtgggtacgg tacgcccgaa catcggctcg ccctctctga
    18361 actcggtccc tgccgggtcc accggctgag ctatgccggg gtgggagact gaaggacaaa
    18421 tccccagggt caaggggtga agacctcgcc ttgcgctacc tgatccaaaa cggatacacg
    18481 ctcgtggagc gcaactacag aacgcggcga ggggagatcg acctgatagt gagcaaggac
    18541 gataccctcg tcttggttct cagaaacaaa gctatcccta aggacgccgc taacatccag
    18601 cgagccgtcg ggcgagatct cgaccgtgag ccggagcatc ttgtagatct ggtgacgctc
    18661 ctcggagctt aagccgtcca gcgcggcggg tgccatgctg gcgtaggact ccaatagcgc
    18721 gtccctatcc cgctccagat tctctaggac ctcacggcga gtcgttatgg ccctgagttc
    18781 cttctcggcc gtctcacgga agttctcgag ctccgctagg gcctcgttca gctcctcgtc
    18841 ggtcagaact cccttggcgg cgagacgcag gtagccgcga cgctcctggt ctacctcgga
    18901 gagcctttcc atccacgcct tggcctcctg gtcggggtca cctctcagcc cagcgcgctc
    18961 ttgctcgatc atctcttcga gcccggcctt gaggcgctcc gggtccttga gcaaaccgga
    19021 gataagatgc cac
//
LOCUS       DASTKM010000046         3929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_360281, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000046 DASTKM010000000
VERSION     DASTKM010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360281"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFQ10_01895"
     CDS             <1..356
                     /locus_tag="VFQ10_01895"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET9964122.1"
                     /translation="GGVHDCPHGALTARVLGPVCEYNAPAAPERYARIAQALGADTRG
                     LELLDAALAGIEELYRLTEDVGIPTMRELGFSEDEIPMLAQIAYEDPQTVGNPREVTV
                     ADYEEIYHDAFVRGK"
     gene            356..1804
                     /locus_tag="VFQ10_01900"
     CDS             356..1804
                     /locus_tag="VFQ10_01900"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9964123.1"
                     /translation="MYTKTKDYSMYLAGEWATGEARMEATSPATGEQIGTVPEGTRAD
                     VERAIAAANEAWSGWAALSAFERAAAMIRIAEIIEERRDDLAQTLTLDQGKPLAAEAY
                     DEVDELVVYFEMASADVTRLEGAMPPSVDAGKRVMLYKVPRGVVGVISPWNWPYTMPA
                     ELIAPALACGNAVIWAPASSTSVCAVKLAECIVDAGLPSGLFNMITGPGSVVGDEVAA
                     NPGTQAIGFIGSIETGQKVAWRAAGKTLLLEMGGNGPMVILDDADLDAATEASLTSAF
                     LGAGQSCTAGERFLVHERVYDAYLERLGGAIERGIRLGDPFDPITTLGPLNNEPTADK
                     MDRHISDALEGGAELVSGGARAEGFPTDLYYRPTVLGKVTPEMRVSREETFGPIVPAT
                     VISDEEEAIRAVNSSPYGLLSAVFTRDLARGLRFAEAVRAGWVNINASSNHWESHLPF
                     GGRAGSRSGVGRVGGRYAMETFTELKTVIVSL"
     gene            complement(1844..2623)
                     /locus_tag="VFQ10_01905"
     CDS             complement(1844..2623)
                     /locus_tag="VFQ10_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964124.1"
                     /translation="MTSEEQSTEDIRADMVEEAVRVAEEAESRDVALRLLGGVAIKVR
                     AHDGLEPAFEREYADLDWITQKGKSSQAQRFFESLGYVPQTRFNTMYGRERLLYFDEK
                     HDRQVDVLVGNFRMCHEIPFGDRMTMEPLTVPLAELLLTKLQIIELNEKDVGDALALL
                     HDHPVGDQDGDTINAAHIAKLCASDWGLWRTFTANLEALGGYLERFDLKDESKERITE
                     RVRALLARIEEEPKTFGWKMRAKIGDRKRWYDLPEEVEGGP"
     gene            complement(2623..3621)
                     /locus_tag="VFQ10_01910"
     CDS             complement(2623..3621)
                     /locus_tag="VFQ10_01910"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9964125.1"
                     /translation="MQANEGEAAEADYSLRRGSRPKRTDDPPKRAGLVGPLLTLLKTS
                     LLLLIAGALGAGCGFGGGGDLTLGYLGWDENVANSYLTKVLLEEELGYDNVELKLADD
                     VGSVYKDLIEGETDAFLDAWMPNHEQFVEEGRGRIGASKEPWYVDPTRYGIAVPDYMQ
                     DVRSISDLDSSGTDMITGLEPGTVLMKKIQTDVVPRYRLQSNLVEATTPAMLAELEQA
                     YSMKEPFVFLAWSPHWMNQEYEFRYLSDPKNAMGSVDAPQTIHSVTREGLAEDEPAAY
                     ALINAMRLDEDNVGSLELAINKAEDPEAGVRRWLEVRENRELVRPWIEAAKNAQEA"
BASE COUNT          698 a         1291 c         1210 g          730 t
ORIGIN      
        1 cagggggcgt ccacgactgc ccccacggcg ccctgacggc ccgcgtcctc ggtcctgtct
       61 gtgagtataa cgcccccgcc gcgcccgagc gctacgcccg gatcgcgcag gcgctaggag
      121 cggataccag ggggctcgaa ctactcgatg ccgccctggc cggcatcgag gaactctacc
      181 gcctgaccga ggacgtcggg ataccgacga tgagggagtt gggcttctcg gaggacgaga
      241 tcccgatgct ggcccagatc gcctacgaag acccgcagac ggtcgggaac ccccgcgagg
      301 ttacggtcgc ggactacgag gagatctacc acgacgcctt cgtaaggggg aagtgatgta
      361 cacgaagacc aaagactact cgatgtacct cgccggtgaa tgggccaccg gcgaagcccg
      421 catggaggcc acgagcccgg cgacgggaga gcagatcgga accgtacccg aagggacccg
      481 tgcagacgta gagcgggcga tagccgccgc gaacgaggcc tggtcggggt gggcggcgct
      541 ctccgcgttc gagcgtgccg cagcgatgat ccggatcgcg gagattatcg aggaacgccg
      601 cgacgacctg gcacagacgt tgaccctaga tcagggcaag ccgctggcgg ccgaggctta
      661 cgacgaggtc gatgagctcg tcgtttactt cgagatggcc agcgccgatg tgacgcgact
      721 ggagggggcc atgccgccct cggtagacgc cggcaagcgc gtcatgctct acaaggtccc
      781 gcgcggggta gtcggcgtga tctcaccgtg gaactggcca tacaccatgc cggcggagct
      841 gatcgcgccg gcgctcgctt gcggcaacgc ggtcatttgg gcgcctgcct cctcgacgtc
      901 cgtctgtgcc gtgaagctag ccgagtgcat cgtcgacgcc ggactgccgt ccgggctctt
      961 caacatgatc accggccccg gttccgtcgt cggggacgag gtggcggcca acccaggcac
     1021 ccaagcgatc ggttttatcg gctccatcga gaccgggcag aaggtggcct ggcgtgccgc
     1081 cggcaagacg ctactcctcg agatgggggg caacgggccg atggtgatcc tggacgatgc
     1141 cgacctcgac gccgccacgg aggcgagcct cacctccgcc ttcctgggcg ctggtcagag
     1201 ctgcaccgca ggggaacggt tcctggtgca cgagagggtc tacgacgcct acctcgaaag
     1261 gctcggcggt gcgatcgagc gcgggatcag actcggcgac cccttcgacc cgatcaccac
     1321 gctcggcccc ctcaacaacg aaccgacggc cgacaagatg gatcggcaca tctccgacgc
     1381 gctggagggt ggcgcggagt tggtctcagg cggggcccgc gccgagggct tccctacgga
     1441 tctctactac cgcccgaccg tgttgggcaa ggtcactcct gagatgcgcg tctcgaggga
     1501 ggagaccttc ggaccgatag tcccagcgac cgtcatcagc gacgaggagg aagccataag
     1561 ggccgtcaac tcctccccct acggccttct ctccgcggtc tttacccggg atctggcgag
     1621 gggcttgcgc ttcgccgagg cggtgagggc cggttgggtc aacataaacg cctcatccaa
     1681 ccactgggag agccacctcc ccttcggcgg gcgagcgggc tcccgctcgg gcgtcggacg
     1741 tgtcggaggc cgctacgcga tggagacttt taccgagctc aagaccgtca tcgtgagcct
     1801 gtaagagtgg agatcggcga ggtgcacgcc ttcgatccag agatcagggc cctccctcca
     1861 cctcctcggg caggtcgtac cagcgtttgc gatcacctat cttcgcgcgc atcttccacc
     1921 caaaggtctt cggctcctcc tcgatgcgcg cgagcaatgc cctgacgcgt tccgtaatgc
     1981 gctccttgct ctcgtctttc aggtcgaagc gctccaggta tccgcccaga gcctcgaggt
     2041 tggccgtgaa ggtgcgccac aggccccagt cggaggcgca cagtttggcg atatgggcag
     2101 cgtttatcgt gtctccgtcc tgatcgccca cgggatggtc gtgcaagagg gcgagggcgt
     2161 cgcccacgtc cttctcgttg agctcgatga tctggagctt cgtaagtagg agctcagcga
     2221 gcggtactgt aagcggctcc atagtcatac ggtccccgaa ggggatctcg tggcacatcc
     2281 tgaagttgcc gacaaggacg tcgacttgcc tgtcgtgctt ctcgtcgaag taaagcagcc
     2341 gctcccggcc atacatcgtg ttgaaccgcg tctgcggaac gtatcccagg gactcgaaga
     2401 accgctgcgc ctgggaggat ttgcccttct gtgtgatcca atccagatca gcgtactccc
     2461 gctcgaaggc gggctccagg ccatcgtgcg cgcgcacctt tatggccacc cctccaagca
     2521 ggcgaagcgc cacatcccta ctctcggcct cctcggcgac gcgcacggcc tcctcgacca
     2581 tgtcagccct gatatcttcc gtgctctgct cttcgctcgt cactacgcct cctgcgcgtt
     2641 ctttgcggct tcgatccagg gccgcaccag ctccctgttc tctctcactt cgagccatcg
     2701 tctgacgccc gcttccggat cttcagcctt gtttatcgcc agctcgaggc tgcctacatt
     2761 gtcctcgtct agcctcatgg cgttgatgag ggcgtaggct gcaggttcgt cctcggcgag
     2821 cccttcccgg gtgaccgagt gaatcgtctg gggagcgtcc accgagccca ttgcgttctt
     2881 cggatcggag aggtaacgga actcgtactc ctggttcatc caatgcggcg accaggcgag
     2941 aaacacgaac ggctctttca tgctgtaagc ctgctctagc tcggctagca tcgccggcgt
     3001 ggtggcctca acaaggttgg attgcagacg gtaccgtggg accacgtcgg tctgaatctt
     3061 cttcatcagg acggtgccag gttctaaacc ggtgatcatg tcggtccctg aggagtcgag
     3121 gtcggagata ctacgcacgt cctgcatgta atcgggaacg gcgataccgt acctggtcgg
     3181 gtcgacgtac cacggctctt tggaagcccc gatccggccc ctaccctctt ccacgaactg
     3241 ctcgtggttc ggcatccagg catccaggaa ggcatcggtt tcgccttcga tcaagtcttt
     3301 gtagacggac ccaacgtcgt cggcgagctt gagctcgacg ttatcgtagc caagttcctc
     3361 ttcgagcagc accttcgtca ggtaggaatt ggccacgttc tcgtcccagc ccaggtagcc
     3421 gagcgtgaga tccccgccgc caccgaaccc gcagcccgca ccgagggccc ctgctatcag
     3481 cagcagaagc gaggtcttca gcaatgtcag gagcggcccg accagccctg ctctcttcgg
     3541 cgggtcatcc gtccgtttcg gacgactgcc tctccttaag ctgtaatcag cctccgcggc
     3601 ctctccttcg ttagcctgca acgtaggatc gcacgccccg ccgtttacac ctctcccgcg
     3661 gcgaaaacaa aaatgcatgc aaccctcgcg cctcctccac cctaccgggg cgcgaatacc
     3721 gctgaatcat acgtcgttcc cgaacctggt tcaacttgaa tgcgcatcca ggacgacaga
     3781 tgcgtccgaa gtgcaggccc gctctagcac agtgggtgga ccgggagatc ctcggccacg
     3841 acctggtggt aagcatgacc atcgcgggtt aaactgttgg cgctttgcgc ctgtagctca
     3901 ggggatagag cagcggtttc ctaaaccgc
//
LOCUS       DASTKM010000047         5258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_888937, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000047 DASTKM010000000
VERSION     DASTKM010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888937"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFQ10_01915"
     CDS             complement(<1..654)
                     /locus_tag="VFQ10_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9964126.1"
                     /translation="MTGARRPRALITGGAGFIGSHLCERLLSEGYQVVCIDNLSTGIL
                     ENVAPFLASEANFEYIEHDVCLHIDLPGELAEVYHFASPASPADFVRIPIEILKSGAL
                     GTHNCLGLARARGARFMLASTSEVYGDPLVHPQREDYRGNVSTTGPRSVYDEAKRYAE
                     ALTMAYHRHHRLDTRIVRIFNSYGPRMREDDGRMVPNFIRQALLGETLTVYGDGTQTR
                     "
     gene            complement(641..2035)
                     /locus_tag="VFQ10_01920"
     CDS             complement(641..2035)
                     /locus_tag="VFQ10_01920"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET9964127.1"
                     /translation="MEHLDVGVVGTGYVGLATGACLAYVGHRVTCLDNNQERVAGLKV
                     GRVPFYEPGVEELIAKCAGRLRFAGSERLTEVIRGSDILILAVGTPQGGDGSADLSNV
                     ADVARAIGRALGHSTPPRELPLVVVNKSTVPVGSAYYVSTLVDDGIDEAGGEGAEFRI
                     ASNPEFLREGSAVHDSLFPDRVVVGTDSREALDALRALYTPIVEQSFPTEWEPRPKVA
                     VPFVTTDLVSAEMIKYASNAFLATKISFINEVANLCELVGADISEVAYGVGLDDRVGS
                     RFLSAGLGWGGSCFPKDVAALRATAREYDHETPLLDAAAAVNEMQRRRVIHKLQRDLH
                     SLKGRRVTLLGLSFKPNTDDLRGPPSLTIAAALNSLGAKVIGYDPVAGKAAAELMPDI
                     QAVFDLYDALRGAHAAVVVTEWQEVRGLDPKKAASLMREPKLIVDGRNALDATASQEA
                     GLLYRSFGRGYDRG"
     gene            complement(2136..3332)
                     /locus_tag="VFQ10_01925"
     CDS             complement(2136..3332)
                     /locus_tag="VFQ10_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964128.1"
                     /translation="MTTAGSGWTTQVVEHTRRLEDYRKTISRLFPALPTDLLDANSIK
                     DVDAIVLTLFLRCYPHELSVLEVGTSFGVAAFHFAGQPSVLRVFGVEQDPLVTEDAGK
                     ASSIDIARAALAEFADERLKIELRTGELSSAWTKAVQELPEDEPLLAFLGGSRTREAV
                     DADLQAIFDANPRAVAILDHCRGDGGPFVQAGIADCMERAWGKYHFRLFGDLSSGMAT
                     SNLGIVYPDEESADVQRCLAELAHLFSQRLDPLWLASREQELIGIVSTYKDEADTLSG
                     QYQDLIGEHQLLADRNTELQKRISQLEKRKSQLQERNASLKMEKRGLQSRTSQLERRI
                     SQLEKRTSQLEERNSRLKEERLLVVSKLSDYRSAGRYKLADAVTKNVLRIPGAKAVAR
                     RTRPKE"
     gene            complement(3357..4250)
                     /locus_tag="VFQ10_01930"
     CDS             complement(3357..4250)
                     /locus_tag="VFQ10_01930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET9964129.1"
                     /translation="MSEAKIVQSTNRIEATVLADSGFGTLHRESKADNGVTIFIPNWN
                     HRPYLPRSIRSALGAIDRLEEAGFSGELVVIDDASRDGSQKFLRTVQVLYEDAPLKTV
                     FLERNLGLPRLRNLAMRMSRYRYVCMLDADNELVADNLPLFLQSGVQTDAAFVHGLLI
                     DKREGRMTGIRSDNVASMRLTAGNYIDAFALLDATKVLRAGGYVTDQRLYGYEDWEMI
                     LHLIAEEEEVVFVPVVMGYYHLTPGSMYAETAEDKGPRPRSAAPEVVTLLRRMFAQTG
                     TREWDAVKVGRIYHPAVGYID"
     gene            complement(4243..5007)
                     /locus_tag="VFQ10_01935"
     CDS             complement(4243..5007)
                     /locus_tag="VFQ10_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964130.1"
                     /translation="MSNTTLLVVSPGQCIEASGDEPLFLSKVACAHKRILASVGPVHS
                     PRYLGRLRRGIHFDALFDIGFASQEDRHSEVSDVPYHFVFNGPIREEEPLAKEPANQA
                     ERTIPWVLVGPRSDRNRDLLGALFELQINPGGVCLLHPRPKNMVPPEPMLGGPQLSAI
                     LSKARYYLWAAERDVVYYESFRFIDPLLAGTVPCKIDPYLTSTGFDVPGVYASVSAFD
                     TEVRDIGYSGMYRRARNFYVSRGRLGEHLSGALRLV"
BASE COUNT          997 a         1610 c         1535 g         1116 t
ORIGIN      
        1 cctggtctgg gtgccatctc cgtagactgt cagggtctcg cccaggaggg cctgtcgtat
       61 aaagttaggc accatcctgc cgtcatcttc cctcattctg gggccgtagc tattaaagat
      121 gcgcacgatt ctcgtgtcca gacggtggtg gcgatgatag gccattgtca gagcctcggc
      181 gtaacgcttg gcctcgtcgt agacgctcct tgggcccgtt gtgcttacgt tgcccctgta
      241 gtcttcgcgc tgcgggtgta cgagggggtc gccgtagacc tccgaggtgg aggcgagcat
      301 gaagcgggcc cccctggcgc gggcaagtcc gaggcagttg tgagtaccaa gtgcgccgct
      361 cttgagtatc tctataggga tgcgcacgaa gtcggctggg ctcgccgggg aggcgaaatg
      421 gtagacctcg gcgagctctc cgggaaggtc tatgtggaga cagacgtcgt gttctatata
      481 ctcgaaattt gcttcgctgg ccaggaaggg ggccacgttc tcgaggatgc ccgtactgag
      541 gttgtcgatg cagacgacct gatagccttc ggagaggagc ctctcgcaga ggtgagagcc
      601 gatgaaccca gcccctcccg taatcagtgc ccgaggcctt ctagcccctg tcatagccac
      661 gtccgaagct cctgtacagg agccctgctt cctgtgaggc ggtcgcatcg agggcgttac
      721 ggccgtccac gatgagcttt ggttcccgca tcagcgaggc ggccttcttg ggatccaggc
      781 ccctcacctc ctgccactcg gtcactacca ccgcggcgtg ggctcctctc agggcgtcgt
      841 agaggtcgaa gacggcttga atgtcgggca tgagctctgc tgcggccttg ccagccacag
      901 ggtcgtagcc tattaccttc gccccgaggg agtttagcgc ggcggctatc gtcaggctgg
      961 gggggcctct caggtcgtcg gtattcggct tgaaggagag cccgagcagg gtcacgcgcc
     1021 tgcccttgag ggagtggaga tctcgctgga gcttgtggat cacccttctg cgctgcattt
     1081 cgtttacggc ggcggccgcg tccaggagcg gggtctcgtg atcgtactcg cgggctgtgg
     1141 cccttagggc ggccacgtct ttggggaagc acgacccgcc ccaaccaagc ccggcggaca
     1201 ggaaacggga ccccacgcgg tcgtcgaggc cgacgccgta ggcaacctcg gagatgtcgg
     1261 cgccgaccag ctcgcagaga ttggcgacct cgtttatgaa gctgatcttg gtcgcaagga
     1321 aagcgtttga agcgtacttg atcatctccg cgctcacgag gtccgtcgtc acgaacggta
     1381 ctgcgacctt ggggcggggc tcccattccg tggggaagct ctgctccact atcggggtgt
     1441 agagggccct gagggcgtcg agggcctccc tggagtccgt ccctaccacc acgcggtcgg
     1501 ggaagaggga gtcatgcacg gcgctgccct ccctcaagaa ctccgggttg gaggcgatcc
     1561 tgaactcagc accctcgcct cccgcctcgt ctatgccatc gtctaccaac gtcgagacgt
     1621 aataggcgct acctacaggc accgtgctct tgtttactac gacgagtgga agctcgcgcg
     1681 gtggagtcga gtggccgagc gcgcgtccta tcgcgcgggc aacgtcggcc acgttcgaga
     1741 ggtcggccga tccatcaccg ccctgcggcg taccgacggc tagaatgagg atgtccgacc
     1801 ctcgtatgac ctcggtcagt ctctcggagc cggcaaaacg gagccttcct gcgcacttgg
     1861 cgattagctc ctcgacgccg ggctcgtaga acgggaccct tcccactttg aggcctgcca
     1921 cgcgctcctg gttgttgtcc aggcatgtca ccctgtgtcc gacataggcg agacacgccc
     1981 ccgtcgccaa cccgacgtag cccgtcccaa cgacacccac atcaaggtgc tccatgcgac
     2041 ctgtttcccc tacatgcctt cggaagtaag cctacagttc ataacagaga tgttacagga
     2101 agggggaata cgcgactagc ctcgacggta ggaccctact ctttcggcct ggtgcgtctt
     2161 gccacggcct ttgcgcccgg tatgcgaagg acgttcttcg ttacggcatc agcaagcttg
     2221 tagcggccgg cactgcgata gtcgcttagt ttcgagacga ctagcaacct ttcctctttg
     2281 agcctggagt tgcgctcttc gagctgggac gtgcgcttct cgagctggga gatgcgcctc
     2341 tcgagttgag aggtgcgact ctgaagcccc ctcttctcca ttttgaggct ggcgttgcgc
     2401 tcttgcagct gggattttcg cttctcgagc tgggagatgc gtttctggag ttctgtgttg
     2461 cggtcggcca gcagttggtg ttcgccgatc aagtcctgat attgcccgct cagagtgtcc
     2521 gcctcgtctt tgtaggtgct tacgataccg atcaactctt gctcccgact cgccaaccat
     2581 agcgggtcca accgttgact aaagagatgg gcgagctctg ccaggcacct ctgtacatcc
     2641 gcagattcct catctggata gacgatccca agattactcg tcgccatacc cgaactcagg
     2701 tcaccgaaga gccgaaaatg gtactttccc caggctctct ccatacagtc tgcgattcct
     2761 gcctgtacga aaggtccccc gtcacccctg cagtggtcga ggatggcaac ggcgcgcggg
     2821 ttggcgtcga agatagcctg aagatcagcg tccaccgcct ccctcgtgcg cgacccgccc
     2881 aagaacgcta gcagcggttc gtcttccggg agttcctgca ctgcctttgt ccaggcgctg
     2941 cttagctcgc ctgtccgaag ctctatcttg aggcgttcat cggcgaactc tgcgagggcg
     3001 gctcgggcga tgtctatact tgacgccttg ccagcgtcct cggtcaccag cgggtcctgc
     3061 tctacaccaa acaccctcaa cacgctgggt tggcctgcaa agtggaacgc ggctaccccg
     3121 aaggacgtac caacctcgag cacactcagc tcgtgcggat aacatcgcag gaagagtgtc
     3181 aggacgatgg catcgacgtc tttgatcgag ttcgcgtcga gcaggtccgt cggtagcgca
     3241 gggaagagcc tgcttatggt tttcctgtaa tcttcgagcc gccgcgtgtg ctcgacgacc
     3301 tgtgtggtcc acccgctgcc ggcagtagtc aagatgcctc ccggtagggg ctaacgtcag
     3361 tcaatgtagc ctaccgcagg gtggtagatg cgccccactt tgacggcatc ccactcccgt
     3421 gtccccgtct gggcgaacat acgccgcagc aaggtcacaa cttcaggcgc agcagagcgg
     3481 ggacgaggcc ccttgtcctc cgctgtctct gcatacatgg atccaggagt cagatgatag
     3541 tagcccatca cgacaggaac gaacacgacc tcctcttcct cggcgatcaa gtgtaggatc
     3601 atctcccaat cttcgtaacc gtagagcctc tggtcggtga cgtacccccc ggctcttagc
     3661 actttcgtag cgtctagcaa ggcgaaggca tctatgtagt taccggccgt aaggcgcatg
     3721 cttgccacgt tgtccgacct tatgcccgtc atcctccctt ctcgcttgtc tatcaacaga
     3781 ccgtgcacga acgcggcgtc tgtctgcacg ccgctctgca aaaacagtgg caagttgtcc
     3841 gcaacgagct cgttgtcggc gtccaacatg cagacgtacc ggtagcgcga catccgcatc
     3901 gccaggttgc gcagccttgg gagaccgagg ttgcgctcca ggaataccgt tttcagcggc
     3961 gcgtcctcgt agagaacctg gaccgtccgc aggaatttct gcgaaccgtc gcgggaggca
     4021 tcgtcgatga ctacgagctc tccagagaaa ccagcctcct cgaggcgatc gatcgccccg
     4081 agggcactgc gtatcgaccg gggaagataa ggtcgatggt tccagttcgg aataaagata
     4141 gttacgccat tatctgcctt gctctccctg tggagcgtac cgaaacccga atcggcgaga
     4201 acagtggcct ctatacgatt tgtgctctgc actatcttgg cctcagacaa gacgcaacgc
     4261 cccggataaa tgctccccca accgtcctct ggagacgtag aagttcctgg cacgacgata
     4321 cattccacta taccctatgt cccgcacctc cgtatcgaag gccgagacag aagcatagac
     4381 gccgggaaca tcgaaccctg tcgaggtgag ataagggtct atcttgcatg gcaccgtccc
     4441 tgccaacagc gggtcgataa aacgaaaact ctcataatac acgacgtcac gttcagcggc
     4501 ccacagataa tatctggctt tcgacagtat cgccgagagt tgcgggccac caagcatggg
     4561 ctctggcggg accatgttct tcgggcgggg atgtaagagg cagactcccc ctgggttgat
     4621 ttggagttcg aacaacgccc ccaggagatc cctgtttcta tcactcctgg gtcccactag
     4681 gacccacggg atcgtacgct ctgcctggtt tgccggctcc tttgccaacg gttcttcttc
     4741 tcggataggg ccgttgaaca cgaagtggta aggtacgtcg gacacctcag agtgtctgtc
     4801 ttcctgagac gcaaaaccga tgtcgaaaag ggcgtcgaag tggatccctc ttctcaggcg
     4861 ccccagatat cttggactat gtaccggacc gacggacgcc aggatgcgct tgtgtgcgca
     4921 ggctaccttc gagaggaata gcggttcgtc accgctcgcc tcgatgcact gccccggact
     4981 cactacaagc aacgtggtgt tactcaaatc ctctgaggcc aaggcgcata cctcgggcga
     5041 cgagtacacc gcaacactgt gtgatgcgtc tctaaagccc ttcgacaggc gcgaagccaa
     5101 gtcctggttg cccgtcgcgg gtggacgtac gattgcgagg gtttcgatct gcatgccaca
     5161 tctacgatat caggcctcat cgttcaatgc tataaaatgc ctcatacaga actctcccgc
     5221 gagggtttag ctactttgag agatcgtttt cggccacg
//
LOCUS       DASTKM010000048         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_184740, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000048 DASTKM010000000
VERSION     DASTKM010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184740"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="VFQ10_01940"
     CDS             <1..1471
                     /locus_tag="VFQ10_01940"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HET9964131.1"
                     /translation="EVAPRLYGSFIEHLGRAIYGGIYELEHSTADDMGFRGDVLELVR
                     ELSVPIVRYPGGNFVSGYDWEDGVGPKEARPRRLDLAWRTVETNQVGTNEFAAWAERA
                     GAEVMMAINLGTRGVDAARNLVEYCNHPSGSHYSDLRRSHGVEYPHGVKVWCLGNEMD
                     GPWQIGRKTPQEYGRLACEAAKAMKWVDPSIELVACGSSYSAMPTFAEWERTVLEHTY
                     DHVEYISLHTYYRNPEDDRATFLAQSLGMDAFIKSVIATCDFVKARKRSKKTINLAFD
                     EWNVWFHSLEADKELEPWSVAPPQLEDVYTFEDALVVGCMLITLLKHADRIKIACLAQ
                     LVNVIAPIMTENGGPAWRQTIFWPFLHASTYGRGTALNLAVESPRYDNDTFGEVPLLE
                     AVATLNDDSGDLTLFAVNRDQEDSLSLEGDLRSFATYEVVEHLVLQHNDPKATNTAKH
                     PHNVAPHDGGDAAMLNGRLTALLPRLSWNVIRMSQASTE"
     gene            1577..>2686
                     /locus_tag="VFQ10_01945"
     CDS             1577..>2686
                     /locus_tag="VFQ10_01945"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HET9964132.1"
                     /translation="MNSAQANERYVVGVDFGTESGRAVVVRASDGAELAGSVCPYQHG
                     VIDRALPESDHPLPPEWALQDPDDYIAVLRTAVPRAVEESGVDSSSVVGIGIDFTSCT
                     LLPTTSDGTPLCSLPEWRDEPHAWVKLWKHHAAQPQADRINDLAEEMGQTWLPRYGGK
                     YSSEWFYSKALQILEEAPGVYKAADRLIEAADWVIWQLCGDETRNACTAGYKAIYQDG
                     HFPTREFLGALHPEFADIVDTKMKRELLPLGSRAGDLTEEAARWTGLQPGIAVAVANV
                     DAHVSVPAAEVVESGRMVMVMGTSTCHLMLSNNLSEVPGMCGVVDGGIVPGLYGYEAG
                     QSGVGDIFAWFVKHAVPPEYHEAARTRGIDLHDHLE"
BASE COUNT          540 a          798 c          861 g          487 t
ORIGIN      
        1 cgaggtggcc ccgcgcctct acggatcctt tatagagcac ctcggacggg ccatctacgg
       61 cggcatctac gagctcgagc actccactgc cgacgacatg gggtttcggg gtgacgtcct
      121 cgaactcgtc cgggagctaa gcgttccgat cgtgcgctac cccggcggca acttcgtctc
      181 cggctacgac tgggaggatg gcgtggggcc taaggaggcg cgcccgcggc gcctggatct
      241 cgcctggaga accgtggaga ccaaccaggt tggcacgaac gagttcgccg cgtgggcaga
      301 gcgagccggc gccgaggtga tgatggcgat caacctcggc acccggggcg tggacgccgc
      361 caggaacctc gtcgagtact gcaaccaccc ctcgggaagc cactacagcg acctaagacg
      421 ctcgcatggt gtggagtacc cgcacggagt caaggtgtgg tgcctgggca acgagatgga
      481 tgggccgtgg cagatcggtc gaaagacgcc ccaggagtac ggccgcttag cctgcgaggc
      541 ggcgaaggcg atgaagtggg tggatccctc catcgagctc gtcgcttgtg gcagctccta
      601 ctctgcgatg cctaccttcg ccgagtggga gaggacggtc cttgagcaca cctacgatca
      661 cgtcgaatac atctcgctgc acacctacta caggaacccg gaggacgacc gcgccacctt
      721 cttggcacag tcgctcggca tggacgcgtt cataaagtcg gtgatcgcga cctgcgactt
      781 cgtcaaggct aggaagcgta gcaagaagac cataaacctc gccttcgacg agtggaacgt
      841 atggttccac tcgctcgagg cggataagga gcttgaaccg tggtcggtcg ctcccccaca
      901 gctcgaagac gtctacacct tcgaagacgc cttggtggtg gggtgcatgc tcatcacgct
      961 actaaagcac gcagacagga taaagatcgc gtgccttgct cagctcgtga atgtcatcgc
     1021 tccgattatg acagagaacg gcggccccgc ttggcgccag accattttct ggcccttctt
     1081 gcacgcctcc acctacggtc gcgggacagc gctgaacctg gctgtcgagt cgccacggta
     1141 cgacaacgat acgttcgggg aggtcccgct gcttgaagcg gtcgccacct taaacgacga
     1201 cagcggtgac cttaccctct tcgccgtcaa ccgggaccaa gaagattcgc tctcgctgga
     1261 aggagaccta agaagcttcg cgacctacga ggtggtcgag cacctcgtcc tgcagcacaa
     1321 cgatcccaag gcgaccaata cagccaagca cccccacaac gtagcgccac acgacggtgg
     1381 cgacgctgct atgctgaacg gaaggttaac agcgctcctg ccccggctct catggaacgt
     1441 gatccgcatg tctcaagcgt cgaccgaatg aggggttcgt atggggacgg cgggcaagcg
     1501 agagcccggt gaacatgcgt gaaggcgttg agcgttgcgc ccccgagact actacgaaaa
     1561 tgagaaggag gcgtgcatga acagcgctca ggcgaacgag agatacgtgg tcggtgtcga
     1621 cttcggcacg gagtcgggta gggctgtagt tgtccgggcg tcggacggtg cggaactagc
     1681 gggttcggtc tgtccctacc aacacggtgt catcgatcgc gctctaccag agagtgatca
     1741 tcctttaccg ccagagtggg ctttgcaaga tcccgatgac tatatagcgg tgctccgtac
     1801 ggcggtcccg agggcggttg aggagagtgg tgtggactcc tcctcagtcg tcgggattgg
     1861 catagacttc acctcctgta cgctgttgcc caccacctcc gatggcacgc cgttgtgctc
     1921 cttgcccgag tggcgcgatg agccgcatgc ttgggtaaag ctctggaagc accacgccgc
     1981 tcagccccaa gcggaccgta tcaacgatct cgccgaggaa atgggacaga catggctccc
     2041 ccgctacggc ggtaagtact cctcggagtg gttctactcc aaggcgctac agatcctcga
     2101 ggaggcgcct ggtgtctaca aagctgctga ccgcctaatt gaggcggctg actgggtgat
     2161 ctggcaactg tgcggcgacg agacccgcaa cgcatgcact gcagggtata aggctatcta
     2221 ccaggatgga cacttcccga cgcgcgagtt tcttggcgcc ttgcaccccg agttcgccga
     2281 catcgtggat acaaagatga agcgtgagct cttaccgctc ggctcgaggg caggagatct
     2341 gaccgaggag gctgcccgct ggaccggcct gcagcccggg atcgcggtgg ccgtggcgaa
     2401 cgtagacgcg catgtgagcg tacccgcggc ggaggttgtc gagtcgggcc ggatggtcat
     2461 ggttatggga acaagtacct gtcatttgat gttgagtaac aacctctccg aggtgccggg
     2521 gatgtgcggg gtggtcgacg gcggcattgt ccccggcctc tatggctacg aagccggcca
     2581 gagcggggtg ggagacatct ttgcctggtt cgtgaagcat gccgtgccgc ccgagtacca
     2641 cgaagcggcg cgtacgaggg ggatagacct tcatgaccat ctcgaa
//
LOCUS       DASTKM010000049         3376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_536932, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000049 DASTKM010000000
VERSION     DASTKM010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536932"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFQ10_01950"
     CDS             <1..351
                     /locus_tag="VFQ10_01950"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9964133.1"
                     /translation="ACFYVRNAERVRGKPRIDLSLDPPPDLVIVVDITSPSLDKFPIY
                     ARAGIPEIWRHDGESLAIFELRGEEYIEVAESKILRPLTSEALSGFIAQRSSLDIVTW
                     MRRVREWSRDNITR"
     gene            404..1045
                     /locus_tag="VFQ10_01955"
     CDS             404..1045
                     /locus_tag="VFQ10_01955"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9964134.1"
                     /translation="METVKSPAEQRVVLHNIGWNTYEHLLADHENNSAPRLTYDRGEL
                     EIMSPSPEHEMFNRRIAQLVLAVAEELNIEAEDLGSTTFRREDLERGFEPDSCFYIQN
                     EEQVRGKDRIDLAVDPPPDLVIEIDITSPSFSKLPIYAQIGVPEVWRYDGERMTILVL
                     EGSDYAERTESIVLPPVTSNVLTDFVEKSKSMKRTAWLVVVREWSRENSGSSS"
     gene            complement(1069..1290)
                     /locus_tag="VFQ10_01960"
     CDS             complement(1069..1290)
                     /locus_tag="VFQ10_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964135.1"
                     /translation="MGLPNVARYPEATVVRDETSILILFGGPYGEQKMSVPLEYVGGD
                     AEAAELRLLAQLQQIGYSVRRGEHEPSDL"
     gene            1390..2250
                     /gene="fdhD"
                     /locus_tag="VFQ10_01965"
     CDS             1390..2250
                     /gene="fdhD"
                     /locus_tag="VFQ10_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HET9964136.1"
                     /translation="MLEKTSGRVRRGSKTKTRVRVVEDGRVRVRPDTLATEEPMEIRL
                     LAGQRMQTVAVTMRTPGSDFELAAGFLYGEGIIDSPDDVQKISYCVDSDLDAEQRYNI
                     VNVELRGGREFDLRPLERHFYTTSACGVCGKASLEQLELRGCPVMPAGPEVSAEVINT
                     LPEKLREAQGLFDATGGLHAAALFDDKGELIALREDVGRHNATDKLVGWALLDGRLPL
                     TDHIIMVSGRSSFEILQKSLAAGAPVVCAISAPSSLAVDVAKQFGMTLVGFLRGGRFN
                     VYAGSERIKP"
     gene            complement(2281..2430)
                     /locus_tag="VFQ10_01970"
     CDS             complement(2281..2430)
                     /locus_tag="VFQ10_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964137.1"
                     /translation="MSEETRQESGGQTSSSIVLVVVLWALVLIPLAWGVYQTLTGVVA
                     LFAGG"
     gene            complement(2441..2911)
                     /locus_tag="VFQ10_01975"
     CDS             complement(2441..2911)
                     /locus_tag="VFQ10_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9964138.1"
                     /translation="MGRKPIYMVYLGVGLVLYFVLAAFGATSLVLFLLVTVVILSFYG
                     GGFATIPAYLKDMFGVEEVGAIHGRLLTAWSCAGVAGPLIINAIADYQISAGRTGADL
                     YTLSLYIMVGVLAVGFVANLLVRPVDPKFHMQSGREEESEKESTASGKAAGSES"
     gene            complement(2930..>3376)
                     /locus_tag="VFQ10_01980"
     CDS             complement(2930..>3376)
                     /locus_tag="VFQ10_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964139.1"
                     /translation="GLAIMGFGGGALIASPLSSTLLETYASNPVDAIVPSFLTLGAIY
                     LVSMMIGAFVVRLPAEGWQPEGWTPPAEEETSDLQTTADVSAGSAIRTPQFWMLWTVL
                     FCNVTAGIGLLEQASPMIQDYFAVEAAAAAGFVGLLSLANMAGRIG"
BASE COUNT          700 a         1053 c         1049 g          574 t
ORIGIN      
        1 gcctgtttct acgttcgaaa cgcagagcgt gttcgaggta agcccaggat agatctgagt
       61 ctggatccac cgccggattt ggtgatcgtg gtagacatca cgagcccctc cctcgacaag
      121 ttcccaatct acgctcgagc aggcataccc gagatctggc gccacgacgg tgaaagtctg
      181 gcgatcttcg agctgcgcgg cgaagaatac attgaggttg ccgagagtaa gatcctccgt
      241 cctctcacga gcgaagccct atccggtttc atcgcgcaaa ggtcctcact agatatcgtg
      301 acctggatgc gccgggtacg cgagtggtcg cgggacaaca tcacccgctg aagcaggcca
      361 gtcctcacga gtcagataac ttctcgcagt ctataatcgc aacatggaga cggtcaagag
      421 tccggcggag cagagggtgg ttcttcacaa catcggttgg aatacctacg aacatctgct
      481 cgccgaccac gagaacaaca gcgcgccgcg tcttacttac gaccggggag agctggagat
      541 catgagcccg tcacccgagc acgagatgtt caatcggcgc atagcacaac tcgtgttggc
      601 cgtggccgag gagctaaaca tcgaagccga agatctcggc tccacgacct tcaggcgcga
      661 agacctggag cgcggcttcg agcccgactc ctgcttctac atccagaacg aggagcaggt
      721 tcgtggcaag gaccgcatag acctggccgt ggacccgcca ccggacctgg tgatcgagat
      781 agacatcacc agtccgtcct tcagcaagct ccccatctac gctcagatag gcgtccccga
      841 ggtctggcgc tacgatggag aacggatgac gatactggtt ctcgaaggtt ccgactacgc
      901 agagagaacc gagagtatcg tcctcccgcc cgtcacgagc aacgttctta ccgacttcgt
      961 cgagaagagc aagtctatga agcgcacggc ctggctcgta gtggtgcgcg agtggtcgcg
     1021 ggagaacagc ggctcgtcta gctgaacacg cccttcctta tcctgcggtc agagatctga
     1081 gggttcgtgc tccccacgcc tgacgctgta gccgatctgc tgtagttgcg cgagcagtcg
     1141 cagctccgcc gcctcagcat ccccaccgac gtactcgagc gggacgctca tcttctgttc
     1201 gccgtagggg ccgccgaaga gaatgaggat gctcgtctcg tccctgacga cggtcgcctc
     1261 ggggtaacgg gcgacgttgg ggaggcccat caatctcaag ctatttgccc gatctcatcg
     1321 tacatcgttc gcgcatgcta acacgccgcc gctgatacac tctgagaaag atcgcaccag
     1381 gagaggccga tgctggagaa aacctcgggc agagtcaggc gcgggagcaa gacgaagacg
     1441 cgggtaaggg tcgtcgagga cggtcgggtg cgcgtgcggc ccgacacgct agcgaccgag
     1501 gaaccgatgg agataagact cctcgccggg cagaggatgc aaaccgtcgc cgtcacgatg
     1561 cgcacgccag gatcggactt cgagctcgcc gcggggttcc tgtacggcga aggtatcatc
     1621 gactctcccg acgacgttca gaagatcagt tactgcgtgg actctgacct cgacgccgag
     1681 cagcggtaca acatcgtgaa cgtggagttg cgcggaggcc gggagttcga tctgcggccg
     1741 ctggagaggc acttctacac cacgagcgcc tgcggcgtgt gcggcaaggc cagcctggag
     1801 cagttggaac tgcgcggctg ccctgtcatg ccggccgggc ccgaggtttc ggctgaggtc
     1861 atcaacaccc tgccggagaa attgcgcgag gctcaggggc tcttcgacgc gacgggcggt
     1921 ctacacgcgg cggccctctt cgatgacaag ggggagctta tcgcgttgcg ggaagatgta
     1981 ggaagacaca acgcgacgga caagctcgtg gggtgggcgc tcctggatgg caggctgccg
     2041 ctcacggatc acatcattat ggtcagcggc cggagcagct tcgagatcct gcagaaatcc
     2101 ctggccgccg gggcccccgt cgtgtgcgcc atctcagccc ccagcagcct ggccgtcgac
     2161 gtggcgaagc agttcggtat gacgctggtc ggcttcctac gcggaggtag gttcaacgtc
     2221 tacgccggaa gcgagaggat aaagccgtag aaaaatcggg cgcgagacac gcccgacaag
     2281 ctatcccccc gcgaacaggg ccacgacgcc cgtaagcgtc tggtaaacac cccacgcgag
     2341 cggtatcagg accagggccc acagcactac gacaaggacg atgctcgagg aggtttgccc
     2401 tccgctctcc tgccgcgttt cttcgctcat ggcgttcctc ctacgattcg ctgccggcgg
     2461 ccttgccgga ggcggttgac tccttctcgc tctcctcttc ccgtccagac tgcatgtgga
     2521 acttcgggtc aacgggcctg accagcaagt tggcgacgaa gccaactgcc agcacgccga
     2581 ccatgatgta cagggagagc gtgtacagat ccgccccggt gcgcccggct gagatctggt
     2641 agtcggcgat ggcgttgatg atcagcggcc ccgcgacgcc ggcgcacgac cacgccgtca
     2701 ggagcctccc gtggatggcc cccacctcct cgacaccgaa catgtccttg aggtaggcag
     2761 ggatcgtggc gaatccgccc ccgtagaagc tcaggatcac cacggtcacc agtaggaaca
     2821 gcactagcga agtcgctccg aaggcggcca gcacgaagta aaggaccagg cccacgccca
     2881 ggtacaccat gtagatgggc ttgcgtccga tgtagtccga ggtcgaggat cagcctattc
     2941 gtccggccat gttggccagc gacagaagtc ctacgaaccc agccgccgcc gcggcctcga
     3001 cggcgaagta gtcctggatc atcggcgagg cctgctccag aagaccgatc cccgccgtca
     3061 cgttgcagaa gagcaccgtc cataacatcc agaattgcgg cgtgcggatg gcgctgcccg
     3121 cagacacgtc ggcggtggtc tgcaggtcgc tggtttcctc ctccgcggga ggagtccagc
     3181 cttcgggctg ccaaccctcc gcgggcaggc gcaccacgaa cgcgccgatc atcatagaga
     3241 ccagataaat agcgcccagc gtcaggaagg agggcacgat cgcatccacc gggttactgg
     3301 cgtaggtttc gagcaacgtg ctcgagagcg gcgaggcgat cagcgcgccg cccccaaagc
     3361 ccatgatagc gaggcc
//
LOCUS       DASTKM010000050         6977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_890063, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000050 DASTKM010000000
VERSION     DASTKM010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890063"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /gene="fba"
                     /locus_tag="VFQ10_01985"
     CDS             complement(<1..1022)
                     /gene="fba"
                     /locus_tag="VFQ10_01985"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01521.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET9964140.1"
                     /translation="MALVTMRQLLDEAANGGYGVGAFNVNNLEQVQAIMEAARETESP
                     VIIQASRGARKYANDRFLYHLMEAATEVYPEISVALHQDHGNSPETCKSAIDLGFTSV
                     MMDGSLMDDGKTPADFEYNVNVTREVVEMAHASGVTVEGELGTLGGIEDDVGSGEVNL
                     TDPDQAVEFVERTGVDALAVAIGTSHGAYKFTKEPDREVLAMHIIEEINERLPTTHLV
                     MHGSSSVPRELVDIINEYGGDIQPTWGVPVREIQEGIKHGVRKINVDTDLRLAATGAI
                     RKAFAEDPSKFDPRYYLKPARAAMQRVVKARMEAFGQAGHAKDYDPISLPDMAVRYRE
                     QGHQLTV"
     gene            complement(1258..2058)
                     /locus_tag="VFQ10_01990"
     CDS             complement(1258..2058)
                     /locus_tag="VFQ10_01990"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HET9964141.1"
                     /translation="MGKRSDRRKFRRRRRWVAFFLLLSLALMVFGVMLLIGMGSPDQR
                     PDASGGLERAASVHVSDAPEAVSGQKRDKKSAAEKNEVDKEAAQKSDEKEVAGDQASQ
                     KPEQKKQSGIPTPPTNDLWMSIPALGLYDNYVTNSAAQSAMDYGAIKLPHTAFPWQDN
                     ANTYIAAHRLGWPGTASDHQFYNLPLLAEGDVIYLGDVNGTTYTYEVTGFKEITPDET
                     WVTTPQPGRDMVSLQTCIENFGDYWTMGPNWYVRYIVQADRVSVDVAK"
     gene            2425..3390
                     /locus_tag="VFQ10_01995"
     CDS             2425..3390
                     /locus_tag="VFQ10_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9964142.1"
                     /translation="MNTETERTKRALSGESTLVARGLKKSYSGFEAVKGVDFRVSGGE
                     CFGFLGPNGAGKTTTMKMIYGAVIPTGGELAVAGLDVYHHEREIKRRIGVVPQENNLD
                     DELKVEENLLIYGRYYDLPSKLVLRRAEELLDFMQLSEKAEATVEQLSGGMKRRLLIA
                     RALLNDPEIVVLDEPTTGLDPQARHLVWDRLRELTDEGKTLILTTHYMEEAARLCDRL
                     VIMEGGLIIAEGTPGALVEEHVSPQVLEFRTDHRSLEGLVPILEAASDAVERTGDESI
                     LAYTDDADALLERVRDSGTEIENTVYRQAGLEDVFLRLTGRRLVE"
     gene            3390..4187
                     /locus_tag="VFQ10_02000"
     CDS             3390..4187
                     /locus_tag="VFQ10_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9964143.1"
                     /translation="MGTIRIPSARGSFRVWQRNITIFQKYWKTILFPNFVEPLLYLAA
                     LGLGLGAFIQQGGIDGQPYVQFVAPGLLASNAMFAASFESTFNTFVKLKIDKIYDAII
                     TTPINAEDVVGGEYLWAGTRAALYGTAFLATIVVLGIVFGQPLVTSWWALLLPVMLLV
                     IGIMFSVMGTLFTSLIERIDLYAYYFTLVVTPLFLLSGVFFPVDDFPAPVPQVAWFTP
                     LYHAVNVCRELATGPSVGVLVDVAWIVGFTGALALIPVQIMRRRLIG"
     gene            4213..4836
                     /locus_tag="VFQ10_02005"
     CDS             4213..4836
                     /locus_tag="VFQ10_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9964144.1"
                     /translation="MEAEQFTVRLARPDDDQAIAALVVEGFLDQFRHVFGGRMDRSLK
                     VMERWVRLEHTSGGVTSLVVEGYADGEIAGSIGVRTEISREDILARGLWRALTRNLGL
                     PRAIWATTLLSYPTYSSIASEAYIERLVISPSFRRQGMARRLLSAAEDLARDAGKKSV
                     GLHVTGNNLGALRLYEAEGYVEVSQQRSLIAAWFMDIREWLYLKKEL"
     gene            complement(4998..6341)
                     /locus_tag="VFQ10_02010"
     CDS             complement(4998..6341)
                     /locus_tag="VFQ10_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9964145.1"
                     /translation="MGITLIVLFVVVAVILAASIVLGRDAREVESARDTEYLELEGVW
                     IRYDVIGGGPAVVLVHGWLSSSRIWEQLAARLAQRFTVYTLDLPGFGESDKPLSGYGI
                     RNGSRLLYAFCAHFGLTRANVIGHDLGGAMAVKLAADHPDVVGRLVVVSAPADEDQID
                     LPTMLWLVTVPVVGPIFYALGRVARPVRRLWMRPFVADSDDLTDVIVDDAGRSTPAAV
                     SKTLSISRRELSRGRLARQARIIKIPLLVAAGEEDQIVDPQSVGVWAGGVERAEICLI
                     DECGHMPMVERIAEFNARILAFLTGDARYLDYVHPVTETNEVVETHQVEGGEEEYATT
                     DPSDDTVDLSPPPTELEPERDSAPRVVRKRGGRYPARSRPQQPYPEPVEEQPTSGDRA
                     RREPSDEDVLPDLPDDLFDWPETRREFGPRERRQDGISDGQDGDSPSDQERPPRL"
     gene            complement(6492..6800)
                     /locus_tag="VFQ10_02015"
     CDS             complement(6492..6800)
                     /locus_tag="VFQ10_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964146.1"
                     /translation="MGLGDIAPGLIVRHTNRRLGKLDPYEACERLISGRISFSEFVDQ
                     VGEEYRELLDFMMDNDLHPGRPEEVLFCIDCQAGLYDRTLLRSQLECLYSHDRRERIG
                     "
BASE COUNT         1272 a         2190 c         2154 g         1361 t
ORIGIN      
        1 accgtcagtt ggtggccctg ttctctgtag cggactgcca tgtctgggag tgagatggga
       61 tcgtagtcct tcgcgtggcc tgcctgcccg aaagcctcca tgcgcgcctt tacgaccctc
      121 tgcatcgcgg ccctggcggg cttcaggtag tagcggggat cgaacttcga gggatcctcg
      181 gcgaaggcct tgcggatcgc acccgtcgcc gccagacgca ggtcggtgtc gacgttgatc
      241 ttcctcacgc cgtgcttgat gccctcctgt atctcccgca cgggcacgcc ccacgtgggc
      301 tgtatgtcgc cgccgtactc gttgataatg tcgacgagct ctctgggcac gctggaggag
      361 ccgtgcataa cgaggtgcgt ggtgggaagc cgctcgttga tctcctcgat gatgtgcatg
      421 gcgaggacct cgcgatccgg ctctttggtg aacttgtaag ccccgtggct cgtcccgatg
      481 gcgaccgcca aagcgtctac ccccgtccgc tcgacgaact ccacggcctg gtcgggatcc
      541 gtcaggttca cctcacccga gccaacgtca tcctctatgc cgcctagcgt ccctagctcg
      601 ccctctacgg tgacgcctga ggcgtgcgcc atctcgacca cctcgcgcgt gacattcacg
      661 ttgtactcga aatccgcggg ggtcttgccg tcgtccatga gcgagccgtc catcatgacg
      721 ctggtaaagc ctagatctat ggcgctcttg caggtctcgg gggagttgcc gtggtcctgg
      781 tgcagggcaa cggagatctc cgggtatacc tccgttgcgg cctccatgag atggtacaga
      841 aagcggtcgt tggcgtactt gcgcgcgcct cgcgaagcct ggatgatgac gggagattcg
      901 gtctcccgcg cggcctccat gatcgcctgg acctgctcca ggttgttgac gttgaacgcc
      961 ccgactccgt agcccccgtt cgccgcctcg tccagaagct ggcgcatcgt aaccagtgcc
     1021 atgtgagtta ccctcctatc tcgaactacc agggccagcc accgcgacgg gagcggccag
     1081 tatagacgct attgcgcaac gtaccaaaga aagagttccc ctggcaaggg acgcgatagg
     1141 gggagtgtgc ccggcgccac tcctacagcg attcgtggca gaacgacgga ccgccacgaa
     1201 tcgcgtgttc ggcccacgag ccgagaggtc gtaggcgaga acaagaagcc gaaatactta
     1261 cttggcgacg tccacggaga ccctgtcggc ctgcacgatg tagcgcacgt accagttcgg
     1321 gcccatcgtc cagtagtcac cgaagttctc tatgcacgtc tgcaacgaga ccatgtccct
     1381 tcctggctgg ggagtggtta cccacgtctc gtccggcgtt atctctttga accccgtaac
     1441 ctcgtaagtg taggtagtcc cgttgacgtc gccgaggtag atcacatccc cctcagccag
     1501 aagcggcaga ttgtagaact gatggtcgct cgcagtgcca ggccacccga gcctgtgcgc
     1561 cgcaatgtag gtgttcgcgt tgtcctgcca ggggaaggcc gtatggggca acttgatggc
     1621 cccataatcc atcgcggact gggccgcaga gttggtcacg tagttgtcgt agagcccgag
     1681 cgccgggatg gacatccaca gatcgttcgt gggcggcgtc ggtatcccag actgcttctt
     1741 ctgttcaggc ttctgcgacg cctgatcgcc ggcgacctcc ttctcgtccg acttctgcgc
     1801 ggcctccttg tcgacctcgt tcttctcggc cgcggacttc ttgtccctct tctgcccgct
     1861 gacagcctca ggggcatcgg acacgtgtac agacgccgcc ctctccaagc caccggaggc
     1921 gtccggtcgc tggtcagggg agcccatccc aatcaacaac attacgccga agaccataag
     1981 ggcaagcgac aacagcagga agaacgcgac ccacctgcgt cgcctcctga acttgcgtcg
     2041 atcagatctt ttgcccatgt gccctctccg tccttcccac ctgcgcaaaa gctgtacacg
     2101 atccgcgtcg ctgccgcttc gccgaacgaa acatgcgcgg gattttagca gcacttttcg
     2161 caaagcggaa gagtaactag tgaacatttc ttaatgattg gcaaagtttc tccgccgatg
     2221 gcagtgagcc tggcgggccg tatactccgt gggacgctga tgcctggccc tggtcggaga
     2281 gccgagatac ggtagtatat gtgccgttcc cgagatcctc cccgaccctg gcgtggtccc
     2341 aaacgccggc cgcttcgggt gagtggcggc ggttaccggg gtcgggactg gtgaacgttt
     2401 ccaagaatat agagaggttg gcggttgaac acggagacgg agagaaccaa gagagccctg
     2461 tctggggagt cgacccttgt ggcgcgaggt ctgaagaaga gctactcagg cttcgaggcg
     2521 gtcaagggtg tcgacttcag ggtctccggt ggagagtgtt tcggtttttt gggcccgaac
     2581 ggggctggga agacgacgac catgaagatg atctacgggg ccgtaattcc gacgggcgga
     2641 gagctcgctg tcgccggtct cgacgtctac caccacgagc gggagatcaa acgccgcatc
     2701 ggggtcgtcc cccaggagaa caacctcgac gacgagctca aggtagagga gaacctactc
     2761 atctacggca ggtactacga ccttcccagc aagctggtac tcaggagggc tgaggagcta
     2821 ctcgacttca tgcagctctc cgagaaggcc gaagcgacgg tcgagcagct ctctggcggg
     2881 atgaagcggc gcttgctcat cgcccgggcc ctgctcaacg accctgagat cgtcgtcctc
     2941 gacgagccga ctacgggact cgacccacag gcccgccatc tcgtctggga ccgcttgcgc
     3001 gagctcacgg atgagggcaa gaccctcatc ctcaccaccc actacatgga ggaggccgcg
     3061 cggctgtgtg acaggctcgt catcatggaa gggggtttga tcatcgccga aggaacgccg
     3121 ggggcgctgg tcgaagagca tgtcagcccg caggtcctgg agttcaggac ggaccacagg
     3181 tcactcgaag ggctcgttcc cattctcgag gcggcctcag acgccgtcga gcggaccggt
     3241 gatgagtcga tcctcgccta caccgacgat gctgatgccc ttctagagcg ggtgcgggac
     3301 tcggggacgg agatcgagaa caccgtttac cgccaggcag ggctggagga cgtcttcctg
     3361 cggctcacgg gccggaggtt ggtcgaatag tgggtacgat aaggataccc tcggcgcgcg
     3421 ggtcgttcag ggtgtggcag cgcaacatca ctatcttcca gaagtactgg aagaccatcc
     3481 tgttccccaa cttcgtcgaa ccgctccttt atctggcggc cctcgggctc gggcttgggg
     3541 cgttcatcca gcagggaggc atcgacggcc agccctacgt ccagttcgta gcgcccgggc
     3601 tcctggcgag caacgccatg ttcgccgcct ccttcgagag cacgttcaac accttcgtca
     3661 agctcaagat agacaagatc tacgacgcca taataaccac gcccatcaac gcggaggacg
     3721 tggtcggcgg tgagtacctg tgggcgggga cgcgcgcagc gctctacggc actgcgtttc
     3781 tcgcgacgat agtggtgctg gggatagtat tcgggcagcc gctcgtcacg tcgtggtggg
     3841 ccctgctgtt gcccgtgatg ctgctcgtga tcggcattat gttcagcgtg atgggcacgc
     3901 tatttaccag ccttatagaa cgcatagacc tctacgccta ctactttacc ctggtcgtga
     3961 cgccgctttt tctgctctcc ggcgttttct ttcccgtgga cgacttcccc gcgccggtcc
     4021 cacaggtcgc ctggttcacc ccgctctacc acgcggtcaa cgtgtgcagg gagctcgcta
     4081 caggaccttc cgtgggagtg ctcgtcgatg tggcctggat cgtcgggttt actggagcgc
     4141 tggccctgat cccggtccag atcatgcgca ggcggctcat cggctgaggg tcggctctgg
     4201 taggatggtc atatggaggc cgagcagttc accgtacgcc tcgcccggcc tgatgatgac
     4261 caggctatcg ccgccctcgt cgtggaagga ttcctggacc agttcaggca cgtcttcggc
     4321 ggtaggatgg accgctcact caaggtcatg gagcggtggg tccgcctgga gcacacctca
     4381 ggaggggtga cctctctggt ggtcgaaggg tacgcagatg gggagatcgc cggtagcatc
     4441 ggcgtcagga ccgaaatctc cagggaggat atcctcgccc gcggtctgtg gagggctctc
     4501 acccgcaacc tcggccttcc gcgagcaatc tgggcgacga ccctgctttc ctacccgaca
     4561 tactcctcca tcgcttcgga ggcctacatc gagcggctgg tgatctcccc ctccttccgg
     4621 cgccagggca tggcacggcg gctgctatcg gcggcggagg acctcgccag ggacgcgggt
     4681 aagaaatccg tcggcctgca cgtgacgggc aacaacctgg gcgctctgcg gctctacgag
     4741 gctgagggtt acgtggaagt ctcgcagcag agatcattga tcgccgcctg gtttatggat
     4801 atccgcgagt ggttgtatct gaagaaggag ctctaaaggg atctgcattt cagcacgccg
     4861 cttcgcggct ctcagcattt cagctagtca gctttcgccg tgagatctca cccttgctgc
     4921 acctagccag acgacttgac ggcaggcgcc acgcagcgac gaaccgcgct accatgctga
     4981 ctagctgaaa tgctgttcta aaggcgggga ggtctttcct ggtcagaggg ggagtcgccg
     5041 tcctggccgt ccgaaatgcc atcctggcgc cgctcgcggg gcccgaattc gcgccgcgtc
     5101 tcgggccagt cgaagagatc atccggcaga tcaggtagaa catcttcatc agagggctca
     5161 cgtctggccc gatcacccga agtcggttgc tcctcgacgg gctcagggta tggttgctgc
     5221 ggccggcttc tggccggata ccttcctccg cgtttgcgca ctaccctcgg agcgctgtcg
     5281 cgttcaggct cgagttccgt ggggggtggg ctgagatcca ccgtatcgtc cgatgggtcc
     5341 gtggtagcgt actcctcctc gccaccttcg acctggtggg tttcgaccac ttcgttcgtc
     5401 tctgtgaccg ggtggacgta atccaggtag cgggcgtcgc ccgtcaggaa ggcgaggatc
     5461 cgggcgttga actcggctat acgctcgacc ataggcatgt ggccgcactc gtctataagg
     5521 cagatctcgg ccctctccac gcccccggcc cataccccga ccgattgagg atcgacgatc
     5581 tgatcctctt ccccggcggc gacgagaaga gggatcttta tgatcctggc ctgcctggcg
     5641 agccggcccc gggagagctc acgcctcgag atactcaagg tcttggagac ggcggcgggc
     5701 gtggaccggc cggcgtcatc cactatcaca tctgtgaggt catcggagtc ggcgacgaag
     5761 ggccgcatcc agaggcggcg gacgggacgt gccacgcgcc ccagggcgta gaagatggga
     5821 ccgactacgg gtacggtgac gagccacagc atggtcggaa ggtcgatctg gtcctcgtcg
     5881 gcaggagctg agacgacgac gaggcggccg acgacatcgg gatggtcggc cgcgagcttt
     5941 acggccatgg ccccgcctag gtcgtggccg atgacgttgg cgcgggtcag tccgaagtgg
     6001 gcacagaaag cgtagagaag cctgctcccg tttcgtatcc cgtatccgga gagtggcttg
     6061 tccgactccc cgaaaccggg caggtcgagg gtatacaccg tgaagcgctg ggccagacgc
     6121 gctgcgagtt gctcccagat gcgggacgag gataaccatc cgtgtacgag tacgaccgcc
     6181 ggccccccgc cgatgacatc atagcggatc cagacccctt caagctccag gtactccgtg
     6241 tcacgggcgc tctccacctc cctggcgtca cgcccgagca cgatggaggc tgcgaggatc
     6301 acagcaacca caacgaacaa cactatcaat gtgatgccca tggaggccat gatagcgcaa
     6361 aaacgcgcca acatgataca gccacgacgc gccctactcg tcccgccgtg gtcggccctc
     6421 tccctacatc gacctcacga accgctaccg cagagccagg cctcacgcgg cctgggacgt
     6481 cgcattcccg gtcagccgat cctttcgcgc ctgtcgtgac tgtagaggca ttccagttgt
     6541 gagcgaagga gagtacggtc gtagagacct gcttgacagt cgatgcagaa gagcacctcc
     6601 tcaggacggc cagggtgcag gtcgttgtcc atcatgaaat cgaggagctc cctgtactct
     6661 tcgccgacct gatccacgaa ctccgagaac gagatgcggc cagagatcaa acgctcgcac
     6721 gcttcgtagg gatccagctt gcccagacga cggttggtgt gcctcacgat gagcccaggg
     6781 gcgatgtccc ccaatcccat gccctccttc atcaccaaga cggtcccttt ctagcccccg
     6841 gaggggtcgt tccccgtttt cctgacaccc cttagaccgc cggcacccca tcagcaggcg
     6901 atccgtgcag tctacgttcg ggcgcgcgtt ttgtaaagcg tctcgccgag aaggggcacc
     6961 ccgaacgata tactccc
//
LOCUS       DASTKM010000051         5553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_185660, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000051 DASTKM010000000
VERSION     DASTKM010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185660"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1394)
                     /gene="hisZ"
                     /locus_tag="VFQ10_02020"
     CDS             complement(147..1394)
                     /gene="hisZ"
                     /locus_tag="VFQ10_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET9964147.1"
                     /translation="MDREPPRKFATTPGTRDVLPPESTRLLEVQGLIRDRFKLFGFRE
                     VLTPALEYSEVIEEHGLRDVSYKLFDQDNQMLLLRPEMTTPIARVISQRLAGSPPPHK
                     LSYVLPAYRRASVGRGQSAELYQAGVEVVGSASPKEDASTIALLVDVLQSLGLSAPSD
                     FAIVLGQSAFYAGYLRHVAPDAAKAVTGALAAKDLVAVDDLAGELPADAAAGVRGIPR
                     IVGPAADESLLEEAGRFVVGKQAREALENLREILRHLEAHGVLGAVILDLGLSGRHEY
                     YTGAVYEAYAAGLGFTVANGGRYDNLLRRFGKELPATGFAIYLERLLSVLPEKDPQLL
                     LVLVGGDVSGIAAAARLRKLGVPVLHLSEDLPPKAAVEYARSVEAAWISYPAAGGVKV
                     ATADPAGEFVFMSVESVARMMLP"
     gene            complement(1466..2860)
                     /locus_tag="VFQ10_02025"
     CDS             complement(1466..2860)
                     /locus_tag="VFQ10_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET9964148.1"
                     /translation="MLSVAVAFAVLLVSGVWDSAAREAEGATAKPNIVFILADDMRKD
                     DLRYMPKTRSALGNKGMSFSNAFVSNALCCPTRATIMRGQYSHNTGVWTNKGSNGGVQ
                     AYRNEGHEKDNMATRLDAAGYRTGLFGKYLNEYSGTKDKPRGWDRWFAHTGGAHYYDY
                     EINDDGRIIHYGSTSADYETDVIADHAKTFIGASAKAGEPFFAYVAPRAPHSPATPAT
                     RDKHAYDGLKARRPPSFNEKKVSDKPPWIRKLPRLSDAKKAKIDNRAEKRAETLQALD
                     DLVAGIVGRLADRGVLSKTYIFFTSDNGWHAGEHRIPQEKWRPYEEDVRVPLLARGPG
                     VAAGSTTYKLVVDTDYLPTYTDLADVHTPNYVDGRSLQPVLEGNTTAWRSAILLEAAA
                     NYSPAYRGLRTSDGRKYVEYASGKRELYNLGRDPYELRDSYDPKSPPATLAKRLKKLR
                     SCAAASCLAAENGQ"
     gene            complement(3189..4136)
                     /locus_tag="VFQ10_02030"
     CDS             complement(3189..4136)
                     /locus_tag="VFQ10_02030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9964149.1"
                     /translation="MARTAYRARGKRWSPTSYALRRARRRRFKVRRTSFVLAVFLIVV
                     LFYFWAPLYGSSERVYTQAVLPEAEILPEPPDLRQPDMERVWDAAIAKDSWPDLATHG
                     KTGTSGRRIALTFDDGPDPRSTPRILDSLRKHDLKATFFVLGRRVKERPGLLRRIVKE
                     GHTIGNHAYNHTDMSDLSQKRMRLELRRTQAAVDHALGYHYPMVLMRPPYGNPYLDSS
                     DALPVFRKVVRDQGLFPILWTIDPRDYMLGGHPEDVLQGIVRADETGRKGERDQVLLL
                     HDTNRQTANALPQIIYHYERSGRQFAAVAELLADKYAQP"
     gene            complement(4212..>5553)
                     /locus_tag="VFQ10_02035"
     CDS             complement(4212..>5553)
                     /locus_tag="VFQ10_02035"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9964150.1"
                     /translation="AGGGAVGVEVLRRALFSGARRRYEPWERSPYRDFPNRVLIVGGG
                     FAGYKAAQTLCELTRDRDDVGVMLISRENYFTFWPMLASVIGGDVATQNVAQPLRRTL
                     IRLGASFRRAPLDSVDLGKRRVVAGGKEFPYEHLVLSLGAEPAYFGIPGVEEHCISIR
                     GIGAGEAIVDRVIERYEEVTLAGGEVPDSKLTFMVIGGGATGVEVASELHSLVRDLLV
                     PDYPNINPHRVKIILVDANKEILKELDPALRRVARKKLADLQIEVINNVKAKKVTADR
                     VILGDGREIMVENVIWTAGARASVKLDEIDLPHDERSGLTVDRYMRVVGHDNVWGIGD
                     CAATVDEDGAPVPPNAQAAVQEGETVARNILSVIDGEEPKPFRYKSLGQLVELGSEFA
                     VNDVLGVKFSGHIAALVWRLTYLYKLESPESRARIAADWLLDIFYDPPVTQIRR"
BASE COUNT          944 a         1767 c         1779 g         1063 t
ORIGIN      
        1 gctgacgata aactcggtac cttctgccct gtagatgagc cttcgcccgt tctcgcggag
       61 aacgccagcc gggagtccgg ccatctccag cgccgcgagc gcgtcagcga agatagcgcc
      121 cttgggcact gcgacgcgca atccgctcac ggtagcatca tcctcgccac ggactcgaca
      181 ctcatgaaaa cgaactctcc ggcggggtcg gcggtggcga cctttacgcc tcctgctgct
      241 gggtaggaga tccaggccgc ctccacgctc ctcgcgtact ccacagctgc ctttggagga
      301 aggtcctctg aaagatgcaa cacgggcacg ccgagcttcc gcagccgggc tgctgccgcg
      361 atgccgctga cgtccccgcc gacgaggacg agcaagagtt ggggatcctt ctccgggagc
      421 acggagagca gcctctccag gtagatggcg aagccggtcg ccggcagctc cttcccgaaa
      481 cgcctgagga gattgtcgta gcgcccgccg ttggcgacgg tgaacccgag gccggcggcg
      541 taggcctcgt agaccgcgcc cgtgtagtac tcgtggcgcc cggagagacc gagatccaga
      601 ataaccgccc cgagcacccc gtgcgcctcg aggtgacgca gtatctcccg aaggttctcc
      661 agggcctccc tcgcctgttt tcccaccacg aaacgtccag cttcctcgag gagcgactca
      721 tccgctgcag gaccgacgat gcgtgggatg ccccggacgc cggcggcggc gtccgccggc
      781 agctccccag caaggtcgtc gacggccacg aggtccttcg cggccagggc gccggttacg
      841 gcctttgcgg cgtcaggggc gacgtgtcgc agatagcccg cgtagaaggc cgactggccg
      901 agcacgatgg cgaagtccga gggggcactg aggccgaggc tctgcagcac gtcgacgagg
      961 agcgcgatgg tggaggcgtc ttcctttggg cttgccgagc cgacgacctc gacgccggcc
     1021 tggtagagct ctgcgctctg cccgcggccc acgctcgcgc ggcggtaggc agggaggacg
     1081 taggagagct tgtgcggcgg cgggctgcct gccaggcgct gggagatgac gcgggctatg
     1141 ggggtggtca tctccggcct taggaggagc atctggttgt cctggtcgaa aagcttgtac
     1201 gagacgtccc tgaggccgtg ctcctcgatc acctcagagt actccagcgc gggcgtgagg
     1261 acctccctaa acccgaacag tttgaaccga tcccggatca gaccctgcac ttcgagcaac
     1321 cgcgtggact cgggcggcag cacgtcgcgc gtgcccggtg tggtggcgaa ctttcgaggc
     1381 ggctcacgat ccataagaga agctatctta acctatgggc gaagccttgg cggccccggc
     1441 aatcagatcg ctacgacttc aagaactact gcccgttctc tgctgccagg caactagcag
     1501 cagcacaact cctcagcttc ttgaggcgct tggcaagggt ggccggtggg cttttgggat
     1561 cgtaagagtc gcggagctcg taggggtcgc ggccgaggtt gtacagctcc ctctttccgc
     1621 tcgcgtactc cacgtacttg cgaccgtcgc tcgtgcggag acctctgtaa gccggtgagt
     1681 agttggccgc agcttcgagc aggatggcgc tcctccaggc ggtggtgttc ccctcgagca
     1741 caggctgcag ggagcgtcca tcgacgtagt tgggcgtgtg cacgtcagcc aggtccgtat
     1801 aggtgggcaa gtagtcggtg tcgacgacca gcttgtaggt ggtcgatccc gccgccacgc
     1861 cggggccgcg cgccaggagc ggcacgcgga cgtcctcctc gtaagggcgc cacttttcct
     1921 gcggtatgcg gtgctcgcct gcatgccatc cgttgtctga ggtgaagaag atgtaggtct
     1981 tggagagcac acccctgtcc gccagcctgc ccacgatccc ggccaccagg tcgtcgagcg
     2041 cctgcagcgt ttcggcccgc ttttcggcgc ggttgtctat cttcgccttt ttggcatcgc
     2101 tgagcctcgg cagcttcctt atccagggag gtttatctga gactttcttc tcgttgaagg
     2161 acggcgggcg ccgggccttg agaccgtcgt aggcgtgctt gtcgcgcgtt gctggcgtag
     2221 ccggactgtg aggggccctt ggcgccacgt aggcgaagaa gggctccccc gctttcgcgg
     2281 aggcgccgat gaaggtcttg gcgtgatccg ctatcacgtc cgtttcatag tctgcggagg
     2341 tcgagccgta gtggatgatt ctgccatcgt cgttgatctc gtagtcgtag taatgggcgc
     2401 cgcccgtgtg cgcgaaccac ctatcccagc cccgcggttt gtccttggtg ccggagtatt
     2461 cattgaggta cttgccgaag aggcctgtcc tgtagcccgc cgcatccaga cgcgtcgcca
     2521 tgttgtcctt ctcgtggcct tcgttcctgt aggcctgcac gccgccgttc gaacccttgt
     2581 tggtccagac cccggtgttg tgggagtact ggcccctcat gatggtggcc cgggtgggac
     2641 aacaaagcgc gttggagacg aaggcgttcg agaagctcat gcccttgttt cccaatgcag
     2701 agcgagtctt cggcatgtac ctgaggtcat ctttgcgcat gtcatcggcc aggatgaaga
     2761 cgatgttggg cttggcggta gccccctcgg cctcccgggc ggcgctgtcc cagacgcccg
     2821 acacgagcag tacggcaaag gccaccgcaa ccgacaacat cacagtcctt ctcatgtcga
     2881 acacccccct tggcaagacg gaagagagaa aagttatcgg acccccgccc cggctacagc
     2941 aacgggagaa gcatagcatg ttcgttgggg aaaggatatc ccgccaaagg gcgtaatggc
     3001 ggggtctttc tcggcgaacc tattgccgcg ccgggctcca ggaagcgggc gaggcaaagg
     3061 aacaggcggc ggtgatccgg gtgtcccgca tcaacttcgt ggtgctcgaa gggcgcctgt
     3121 gcaagatgct cggtggagcg gagctcacca tcgagatcgt ggaggccggg tacctgttcg
     3181 gggacgatct acggctgggc gtacttatca gccagcaact cggccacggc cgcgaattgc
     3241 cggccggatc tctcgtagtg gtagatgatc tgcggcaatg cgttcgcagt ttgcctgttg
     3301 gtgtcgtgca aaagcaacac ctggtccctc tccccctttc gccccgtctc gtccgcgcgt
     3361 acgatgccct gcagcacgtc ctccggatgt ccaccgagca tgtagtctct cgggtcgatt
     3421 gtccacagta tggggaagag gccctggtcg cggacgacct tcctgaacac cggcagcgcg
     3481 tccgagctgt ctaaataggg gttcccgtaa ggcggtcgca tcagcaccat cggatagtgg
     3541 tatccaaggg catggtccac ggccgcctgg gtgcgccgca gctcgagccg cattcgcttc
     3601 tggctcaggt cggacatgtc ggtgtggttg taggcgtggt ttccgatggt gtggccttcc
     3661 ttcacgatcc gtcgcaacaa gccgggtctc tccttgaccc ggcgccccag cacgaagaat
     3721 gttgccttca ggtcgtgctt ccgaagcgag tcgagtatcc gcggggtgga gcgcggatca
     3781 gggccatcgt cgaaggtcag ggcgatcctg cgtcccgagg tcccggtctt gccgtgggtg
     3841 gccagatctg gccaggaatc cttggctatc gcagcgtccc agacccgctc catgtcgggt
     3901 tgtcgcaggt caggaggttc gggaaggatc tctgcttccg gcaatacggc ctgtgtatat
     3961 accctttcgg aggagccgta gagcggcgcc cagaagtaga aaagcaccac gatgaggaag
     4021 accgcgagga cgaacgaggt gcgccgaacc ttaaagcgac gacggcgggc gcgccggaga
     4081 gcgtaggagg ttggggacca tcgtttgcca cgtgccctgt acgccgttct cgccatacta
     4141 gagtatgtta gccgttcgtc ggagagggcg cgagacgtcc cagaccggga tcgtagcctg
     4201 cgcccctgcg gctagcgccg aatctgggtg acgggcgggt cgtaaaagat atcgaggagc
     4261 cagtcggcgg cgatcctggc ccggctctcg gggctctcca gcttatagag gtaagtcagg
     4321 cgccagacaa gggccgcaat atggccactg aatttgacgc caaggacgtc gttgacagcg
     4381 aactcgctcc ctagctcgac gagctgccct aatgacttgt agcggaaggg cttcggttcc
     4441 tcgccgtcta tcaccgagag gatgttcctt gctacggtct caccctcctg tacggcggcc
     4501 tgggcgttgg ggggcaccgg cgcaccatcc tcgtccacgg tcgccgcgca gtcgccgatc
     4561 ccccacacgt tgtcgtggcc tacgacccgc atatagcggt ccacggtaag gccacttcgc
     4621 tcgtcgtgag gaaggtcgat ctcgtcgagc tttacgctcg ccctggcgcc cgccgtccag
     4681 atgacgttct cgaccatgat ctcacgcccg tcaccgagta taaccctgtc cgccgtgacc
     4741 ttcttcgcct tgacgttgtt gatcacctct atctgaaggt cggccaactt cttgcgcgcg
     4801 accctcctca gggccgggtc gagctccttg agaatctcct tgttcgcgtc aacgaggatt
     4861 atcttgacgc ggtgcgggtt gatattgggg tagtctggca cgagcaaatc cctgaccagg
     4921 ctgtgcagtt ccgaggccac ctcgaccccg gtggccccgc ccccgataac catgaaggtc
     4981 agcttggagt cggggacctc accccctgcc agcgttacct cctcgtagcg ctcgatgacc
     5041 ctgtcgacga tggcctcacc tgcgccgatc ccccttatgc ttatgcagtg ctcttccacg
     5101 ccgggtatgc cgaagtaggc aggctccgcc cctagcgaga gcacgagatg ctcgtagggg
     5161 aactccttgc cgcctgccac cacacgtcgc tttccgaggt cgacactgtc cagaggagcc
     5221 cgcctgaagc tggcgccgag ccggatcagg gtccggcgca agggctgcgc gacgttctgg
     5281 gtcgccacgt cgccgccgat gacgctggcc agcatcggcc agaacgtgaa gtagttctcc
     5341 cgggagatga gcatgacccc gacgtcgtcg cggtccctgg tgagctcgca gagggtctgg
     5401 gccgccttgt aaccggcgaa cccgccgcca acgatgagca ccctgtttgg aaagtctctg
     5461 tagggcgacc tctcccaggg ctcatacctc cgccgcgccc ccgagaacaa agcccgccgc
     5521 aagacctcga caccgaccgc tccgccaccc gca
//
LOCUS       DASTKM010000052        11968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1242768, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000052 DASTKM010000000
VERSION     DASTKM010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11968
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242768"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1877)
                     /locus_tag="VFQ10_02040"
     CDS             complement(288..1877)
                     /locus_tag="VFQ10_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9964151.1"
                     /translation="MDNRYDVVRPLGSGGMGEVYLARDRVLGRDVALKVLRKQYAGDD
                     EFAERFKREAMSVASLSHPHIVQVYDRGETEEGSSYIAMEYVPGGTLKERISREGSLE
                     VADAAGLGAQVAEALGAAHDRGMVHRDIKPQNVLLAARGGAKVADFGIARAGSSATIS
                     RTGSVMGTAGYMSPEQALGKPATPKSDLYSLGVVLYEALTGELPYTADNPIAVSMKHV
                     NEPLRPPVQLNPRIPRGMNALVTKLLAKDPEDRYADADELADDLWRVNRGLEPVSAGV
                     GRKQGPSTQRTALASPNVTSKMHRPRPGPVPYRRRRRLPWLLIALAAILFTLGVLGSV
                     QAVLGTDATNGWFNVFQGDKRSPGQPAGPEKVKVPEVDGLTRSVAEKRLTEEGLEVGK
                     VTSFPSDSVAAGRVIEPGIVAGTPVEPGTDVNLTISSGPQPSSSASSSASAQSSPESG
                     ATASASASAQPAPREDRQAVQTPAAGANDPRANVPRPKVQKNPPAQSQNAAAPASEPV
                     DDSGGQGRGRGRGRGGGPGED"
     gene            2106..2633
                     /locus_tag="VFQ10_02045"
     CDS             2106..2633
                     /locus_tag="VFQ10_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964152.1"
                     /translation="MATSGVEANAERLLGAFYDLSGGKLTEPVPIGGPEKPAEGAAPR
                     ADLDPDSTECAVAARYLVDQGYIAPADAASGYVITVAGFDMVREMRGMGGPAAPAGRS
                     KMSDKTQKQLVTLLSIGISIGLSQPLARFIEKEIPERRGIKDDLTEAVLQGLVRVVAL
                     VVASVIVRKLASSRR"
     gene            2715..3620
                     /gene="rbsK"
                     /locus_tag="VFQ10_02050"
     CDS             2715..3620
                     /gene="rbsK"
                     /locus_tag="VFQ10_02050"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HET9964153.1"
                     /translation="MADVFVVGSINQDFVLSVERRPAPGETVTDARLATHNGGKGANQ
                     AAAAALLGASVAFLGRVGDDGFGGPLVRVLSDKGVDTSLVREVPGSSTGAAFITVTPD
                     GENAITVAPGANRRLTTDDVDDASQSIGEARVLVAQMEVPPEVVVRAVEVAAESGTRA
                     LVNLAPPFELPRALLEVLDPLVLNEHEAAFLLGDMVEGIDGALSAAPGLLSLGPKSVV
                     VTLGEEGAVFAGGESAQHLTAPKVKVVDTTGAGDAFVGALAARLAADAPLKDAVAYAV
                     RAGAAAVTEAGAQGALPAPETVDAL"
     gene            complement(3636..4463)
                     /locus_tag="VFQ10_02055"
     CDS             complement(3636..4463)
                     /locus_tag="VFQ10_02055"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9964154.1"
                     /translation="MISKELLSIVRPRPSDTGGLVYAGGTERDGMILGGKARSPATGE
                     TYEIKDGYLDLLKARTGADNIANLANFLPGAGRSYEPLWRVRSLSLLTGEPFPNEREL
                     EIIKRLVRPFRGGRYLDLGCSAGLYTRSLSNNLDSGVAVGIDISPSMLEEAVRHARRI
                     GTNPSFIRANAKHLPFFDASFAGATCGGTLNEFGDPARVLRETYRVLEPSGRLAIMGI
                     LRAGTPRGRGLQRFLSTGGIRFFEPDELQSLLDYAGFEPDPPQTYGPVFFSGATRRG"
     gene            complement(4464..5108)
                     /locus_tag="VFQ10_02060"
     CDS             complement(4464..5108)
                     /locus_tag="VFQ10_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET9964155.1"
                     /translation="MLEGVWMPPDGTDKREYPWEVRDADPDALRSAMRHFPTGVTVVT
                     SGREERAEGMTANAVISVSLDPLLFLVSVHKDARINQRIREEGSFTVNLLADDQEGLS
                     RLFASPERSSGLPALHSLGGGYGSVGAPLAAGALAAIDCELAEIYAGGDHDLFLGRVV
                     EVRLGDTRKGPLIYHEGGYPTLKASPRPEESGAFVDSVRGFDARLNRLRDRRRR"
     gene            complement(5117..5836)
                     /locus_tag="VFQ10_02065"
     CDS             complement(5117..5836)
                     /locus_tag="VFQ10_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964156.1"
                     /translation="MSGAPIVIVGGATWWPGSYRNFASLLQEISGSEVHIAPLTPLDW
                     TLGYFRGFGQLVFEVASTVDRALLESDAKKAVLVGHSAGGLACRVYIGGDPPYGGRRF
                     SGHRRVECLITLGTPHTVADGQRLAPITRVNELFPGALHAEAGLRYVSVAGSVADGAS
                     SPRVRKRYERFVQGGRVAGDGVVPVDAALLPGSEILIVDGVHHNRRLGRWYGSDRETV
                     GRWWPPELRVGGSLVGDRRAQ"
     gene            complement(5857..6471)
                     /locus_tag="VFQ10_02070"
     CDS             complement(5857..6471)
                     /locus_tag="VFQ10_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET9964157.1"
                     /translation="MEFAFNAFVTLLVVVDPLGLAPIFAALTRGFSEEQKRASAIRGT
                     LLGALILFLFALAGDVLLEALGIGLPAFRIAGGLLLFLLSLDMVFARPSGMRSTSVRE
                     QEKESYQQDISVFPLAIPLLAGPGAITTVLLFTGGSSAGQVALFMAVLFVILLLTLSS
                     LLLAPRVMRLFGETGADVLSRVLGVLLAALAVQFVIDGLQASIT"
     gene            complement(6488..7240)
                     /locus_tag="VFQ10_02075"
     CDS             complement(6488..7240)
                     /locus_tag="VFQ10_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964158.1"
                     /translation="MEILIAGPFDAPEVGFGLQQALLEEVAADERGPTALMWTASRYV
                     GATRQETRLPGFAAATEAASGLGFPVLVRNSGGGAVAANRGSLSFSLTFPVEALRHGL
                     YERYSEGLDLIASALRRIGVEAEGGEVEGEFCPGAYSVRSGGPWGVKHAGLAQRVTRR
                     SARLEALVLVSETAEVRDVLERVYGLLGLPFRPESIGNLPADVSQVIKALSEELRGRY
                     QTKRGRIGKKTMDRARSLRGEWRVIPDTGSSL"
     gene            7406..8245
                     /locus_tag="VFQ10_02080"
     CDS             7406..8245
                     /locus_tag="VFQ10_02080"
                     /inference="COORDINATES: protein motif:HMM:NF044469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964159.1"
                     /translation="MLQQEAMIERTRRLCREDERLAAAMIYGSFAQGEGDGFSDIEFI
                     LFFRDEELEGLDQEEWVSRISPVGLYFVNEYGNGTAIFQNLVRGEFHFDRASDIERIV
                     GESMRDADWLPSLEDTLILDRTGELGPRLREIVGPPPERDTPGQIRFVCDCFVNWFVF
                     GSNLFARGELARSLDLLGIVRDRLLQIVRVQEKSTLHWFNSAKFLETEISQTSYARYA
                     ACTARLDRDELRQAYLSAWEWGRELMASLAERHGVAPPTALLVRLDRRFAETFSAPGL
                     EDS"
     gene            8242..8805
                     /locus_tag="VFQ10_02085"
     CDS             8242..8805
                     /locus_tag="VFQ10_02085"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9964160.1"
                     /translation="MRWDETQSPWRTLGSRNVYENPWISVREDSVIRPDGEPGIYGVV
                     HYKNTAVGVLPVEEDHVYLVGQYRYPLERYSWEIPEGGCPEDEEPLRAAQRELREETG
                     LEARNWRMLGEAYLSNSVADEYAVWFLATGLVPGERKPEGTEVIGVRCVPLREAVAMA
                     MDGRITDALSTLALTTYALKGYDGAAG"
     gene            8833..9774
                     /gene="cofD"
                     /locus_tag="VFQ10_02090"
     CDS             8833..9774
                     /gene="cofD"
                     /locus_tag="VFQ10_02090"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HET9964161.1"
                     /translation="MKVCALAGGVGGAKLASGLQDVLLPGDLSVVVNTADDFGLWGLH
                     ICPDLDTVMYTLAGISNPETGWGIAGESFETLNMVESYGEETWFKLGDRDLATHVLRT
                     SRMLSGETLTEVTAGLSGALGVESVVLPMSDDPVSTVLETPQGRLEFQEYFVRRGQRD
                     EVLGIELRGIEDARPTERVLATVSRADAIVLCPSNPVVSVGPILALPGMMEALATSDA
                     PKVAVSPIVGGRALKGPADRMLASLGHEVSATGVARMYAGLVDGMVVDRTDEDERAGI
                     EALGMRVLVTESIMRDAEDRARLGSETLEFGAGLVAR"
     gene            9771..10376
                     /gene="cofC"
                     /locus_tag="VFQ10_02095"
     CDS             9771..10376
                     /gene="cofC"
                     /locus_tag="VFQ10_02095"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HET9964162.1"
                     /translation="MSTISAVVPVKDLWGTKSRLAPILDPGARAGLTLYMMGRVVRAI
                     AEAGVEDVCVVSPDWIVLEEAKRRGATPLVQESRGLNPALEEGRRRALGLGASTLLVL
                     PADLPLLDAGDVRAVLQEAGEGPSAVISPDGGRSGTNALLIQPPDVLTFAFGPDSFEA
                     HLGAARGRSLDVRICERPHLAFDLDTAGDLARLGETGVASP"
     gene            10373..11116
                     /gene="cofE"
                     /locus_tag="VFQ10_02100"
     CDS             10373..11116
                     /gene="cofE"
                     /locus_tag="VFQ10_02100"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HET9964163.1"
                     /translation="MRGIEVLGVEGFPEVRPGDDLNRMISGAVARNLRPRDVLVVTHK
                     IVSKAEGRLVDLRTIEPSTLAKGYATRYGKDPRQIEVVFRESRRIVRMDRGIIISETH
                     HGFVCANAGVDASNVPDDDAVCLLPVDPDASARRLRDALAEDPGVEVAVVISDSFGRT
                     WRHGITDVAIGLAGMDPVADYRGQRDPHGFPMEASVLAIADELAAAAELVMGKTDGIP
                     LAIVRGYSYSPASGSARELLMPPERDMFR"
     gene            11359..>11968
                     /locus_tag="VFQ10_02105"
     CDS             11359..>11968
                     /locus_tag="VFQ10_02105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9964164.1"
                     /translation="MDNLRAYPIYLGIRGSFAFFFTLWATVAAVYRIEVVHLDPLRLV
                     LLGTALEVAIFLFEVPTGVFADTYGRRRSVITGCILMGCGFALEGAIPEFIAVLAAQA
                     VWGVGYTFISGALEAWIADEDADRDLGRVYLRGEQADYFGSFVGIPASVLLGLVTLNL
                     PLIVGGVLTITLGLALFLTMPERNFRPSPREGRSSLHHAATTA"
BASE COUNT         2045 a         3772 c         3983 g         2168 t
ORIGIN      
        1 ggcagcgagc ttcacgcgcg cctggccggg gcccggttcg gggatctcca cgtcctcgta
       61 gccaagaacc tcgggtccgc cgaactcgtg tatccggatc gccttcatgc ttctctccct
      121 cccgcaatga actcggccta cacacccacc attgtgtcag caattcagcc agtaagcacg
      181 cttcggcctt tcaggcctca acttgtcagc ttcttgtttt cgctagaagc ccaatgctcg
      241 tgagcggatg cgggattcgc agcaggtcga tacctctgcc aatcgcatca atcctcccct
      301 ggtcctccac cgcgcccacg tcctcgaccg cgaccttgtc ccccgctgtc atcaacaggc
      361 tcacttgccg gtgcagccgc gttctggctt tgtgcaggtg gattcttttg caccttgggt
      421 ctgggtacgt tggccctggg gtcattcgcc ccggccgccg gcgtctggac cgcctggcga
      481 tcctcgcgcg gagcaggctg cgccgaagcg gatgccgagg cggtggcgcc ggattcggga
      541 ctagactgtg cagacgctga cgagctcgcg gatgaggaag gttgtggtcc tgagctgata
      601 gtcaggttca cgtctgtgcc tggctcgact ggggtgcctg caacgatgcc tggctcgatt
      661 accctgcctg cggcgacact atcgctcgga aacgaggtga ccttcccgac ctcgagcccc
      721 tcttcggtga gcctcttctc tgccacgctc ctggtgagcc cgtccacttc gggtaccttc
      781 accttctccg gtccagcggg ctggccagga gatcgcttgt ctccctggaa gacgttgaac
      841 cagccattcg tggcgtccgt acccagaaca gcctggacgc tgcctagcac gccgagggta
      901 aacaggatcg cggccagcgc gatcagcaac cagggcaggc gtcgtctgcg tcgatacggt
      961 accgggcctg gccggggtct gtgcatcttg ctcgtgacgt tcggcgacgc gagggcagta
     1021 cgttgcgtgc tcggtccctg cttccttccg acacccgccg agaccggctc gagaccacgg
     1081 ttcaccctcc agaggtcgtc cgcaagctcg tcggcgtctg cgtagcggtc ttccgggtct
     1141 ttcgccagga gcttcgtcac gagggcgttc atgcccctgg gtatcctggg gtttagttgc
     1201 acgggtggcc gcagcggttc gttgacgtgc ttcatggaga cggcgatggg attgtctgcg
     1261 gtgtacggca gttcgcctgt gagcgcttca tagagcacca ctccgaggga gtagaggtcg
     1321 ctcttggggg tcgcgggctt gccaagggcc tgttcggggg acatgtagcc tgcggtaccc
     1381 atcacggacc ccgtccttga gatggtcgct gaggagccgg ccctggcgat gccgaagtcc
     1441 gccaccttag ccccgcccct ggctgcgagt agtacgttct ggggctttat gtcgcggtgg
     1501 accatgcccc tgtcgtgggc cgctcccagc gcctcggcga cctgcgcccc gagcccggcg
     1561 gcatccgcca cctcgagcga accctccctg gagatcctct ctttgagcgt gccgccaggc
     1621 acgtattcca tggctatata agaggatcct tcttccgtct cgcccctgtc gtagacctgg
     1681 acgatatgcg ggtgggagag tgacgccacg ctcatggcct cgcgcttgaa acgttcggcg
     1741 aactcgtcgt cgccagcgta ctgtttcctg agcaccttca gcgccacgtc gcgcccgagc
     1801 accctgtcac gggccaggta gacctcgccc atgcccccgc taccgagtgg tcgaacgacg
     1861 tcgtagcggt tgtccaagac tgttcttctt gcctctgaca tcctgtcctt ggtccggctc
     1921 taaaacacca accgcgtcat tctacactta cgcatgggac cttacgaagg atgtcttaga
     1981 ggggttgaag gttaggcggt gccagggtcg ggtactgtgg tggtgtctga tagcattcca
     2041 aagaaaggac tcgatggagc gaagaggcgg acgacgagaa gacggaggta cgtaggggtg
     2101 ggatcttggc tacgagcggg gtagaagcga acgcggagcg gcttctcggc gctttctacg
     2161 atctgtctgg agggaagcta acggagccgg tgccgatcgg gggaccggag aagccagcag
     2221 agggcgcagc gccgagggcg gatctagatc ccgactctac agagtgtgcg gtcgcggcca
     2281 ggtacctggt cgaccaggga tacatcgcac cggccgatgc cgcgtccggg tacgtgatca
     2341 ccgtcgccgg ctttgacatg gtgagagaga tgcgcgggat gggcggtcct gcagcgccgg
     2401 cagggaggag caagatgagc gacaagaccc agaagcagct tgtgacgctg ctttccatag
     2461 ggatctccat cggccttagc cagccgctgg ccaggtttat cgagaaggag atccccgaac
     2521 gcaggggtat aaaagacgac ttgaccgagg ctgtcttgca agggctggtc agggtggtgg
     2581 cgctcgttgt tgcctccgtg atcgtgcgga agctggcctc gagccgcagg tagggcaccg
     2641 atcctggaga cctccccaac ggcgacgtta ctccatcctc gagggtttca tgaggcggga
     2701 gatgagattt ctgagtggct gacgtcttcg tagttggttc cataaaccaa gacttcgtcc
     2761 tgagcgtgga gcgccgcccc gcgcctggcg agacggtcac ggacgctcgt ctggcgacgc
     2821 acaacggtgg caagggggcc aaccaggccg ccgcagcggc cctcctcggc gcgagcgtgg
     2881 cattcctcgg ccgggttggc gacgatgggt tcggcgggcc gctggtacga gtgctctccg
     2941 ataagggcgt cgatacgagc cttgtgcggg aggttcccgg ttcgtccaca ggcgcggcct
     3001 tcataaccgt cacccctgat ggggagaacg cgatcacggt cgcccctggc gcgaaccgcc
     3061 gcctcaccac agacgacgta gacgatgctt cccagtctat cggagaggcc cgggtactcg
     3121 tcgcccagat ggaggtcccg cccgaggtcg tcgtgcgggc cgtcgaggtc gcggcagaga
     3181 gcgggacccg cgcgctggtc aaccttgcgc cgccgttcga gcttccgcgg gcactgctcg
     3241 aggtgttgga tcccctggtg ctcaacgagc acgaggccgc tttcctcctc ggagacatgg
     3301 tcgaaggtat agatggtgcc ctatcggcag cccccggact cctgtctctg ggtccgaaat
     3361 ccgtggtggt cacgctcgga gaagagggtg ccgtcttcgc gggcggcgaa tccgcgcaac
     3421 acctcacggc tccgaaagtg aaagtcgtgg atactacggg ggccggagac gcgttcgtcg
     3481 gggccctggc cgcaaggctc gccgccgacg cccctctgaa agacgcggtt gcctacgccg
     3541 tacgggccgg ggcggcggcc gtgacggagg cgggggccca gggagcgctc ccggcccccg
     3601 agacggtcga tgctttgtag ggtgcggcgc tagcgtcaac cccgtcgggt agcccccgag
     3661 aagaagacag gaccgtacgt ctggggcggg tctggctcga agcccgcgta gtcgagcagg
     3721 ctctgtagct cgtcgggctc gaagaacctg atgccacccg tggagaggaa gcgctggagc
     3781 ccgcgtccac gcggcgtgcc tgcacgcagg atacccatga ttgcgagccg cccgctgggt
     3841 tcgaggaccc ggtaggtctc ccgcaggact cgtgcgggat ctccaaactc gttgagggtg
     3901 ccgccgcagg tggcgcctgc gaaagaggcg tcgaagaatg gaagatgctt cgcgttcgcc
     3961 ctgatgaagg agggattcgt cccgatcctg cgtgcgtggc ggacggcttc ctctagcatg
     4021 gaaggggaga tgtctattcc tacggcgaca ccgctgtcga ggttgttgct gaggctgcgg
     4081 gtgtagaggc ctgcggagca tcccaggtcg aggtagcggc cgccgcgaaa ggggcgtacg
     4141 aggcgtttga tgatctccag ctcccgctcg ttcgggaagg gttcgccagt gagcaggctg
     4201 agggagcgga cgcgccagag tggttcgtag ctgcgtccgg cgcctggcag gaaattggcg
     4261 aggttggcga tgttgtccgc gccggtacgg gctttcaaca gatccaggta tccgtctttg
     4321 atctcgtagg tttcgcccgt cgccggggag cgggctttgc cgcccaggat cataccgtct
     4381 ctctcggtac caccggcgta gacaagaccc ccggtatccg aaggacgggg acggactata
     4441 gagagcagct ccttgcttat catctagcgc cgtcttctgt cacgcaggcg gttcaggcgg
     4501 gcgtcaaagc ccctgacgga gtcgacgaag gcgcccgact cttccgggcg gggggaggct
     4561 ttcagggtcg gatatccgcc ttcgtggtag atcagtggtc ccttgcgcgt atcgcccaga
     4621 cggacctcga cgaccctgcc taagaacagg tcgtgatcgc ctcccgcgta gatctcggcc
     4681 agctcgcagt cgatcgccgc cagtgcgccg gccgccagcg gcgcccccac cgagccgtag
     4741 ccgccgccta aagagtggag tgccggaagt ccgctggagc gttcgggcga ggcgaagagc
     4801 ctgctgagtc cttcctggtc gtcagccagg aggttcaccg taaaggagcc ctcctccctg
     4861 atgcgttggt tgatgcgcgc gtctttgtgg acgcttacca gaaagagcag tgggtcgaga
     4921 gagacagaga tcacagcgtt ggcggtcatg ccctcggctc tctcctcccg cccgctcgtc
     4981 acgacagtca cgccggtagg gaaatgacgc atcgcgctcc gcagggcgtc agggtcggca
     5041 tccctgacct cccagggata ctctcgcttg tccgtaccgt caggtggcat ccacacccct
     5101 tctagcaagc cgtaccctac tgcgcacgcc ggtctcccac gagagagcct cccacgcgca
     5161 actctggcgg ccaccatcgc cctaccgtct ccctgtccga gccgtaccaa cggcctagac
     5221 gcctgttgtg atggacgccg tctacgatca ggatctccga gccaggaagc agggccgcat
     5281 cgacaggaac gaccccgtct ccggccaccc taccgccttg gacgaaccgc tcgtagcgct
     5341 tgcggaccct gggtgaggag gcgccgtcgg cgacgctacc agcgaccgag acgtagcgca
     5401 aaccagcttc agcgtggagg gcgccgggga agagctcgtt tacgcgggtg atcggggcga
     5461 ggcgttgtcc atccgcgacg gtgtgtgggg tgccgagtgt gatcaggcac tccaccctcc
     5521 tgtgtcccga gaaacgcctg cccccgtacg gaggatctcc gcctatatat accctgcacg
     5581 ctagaccgcc cgccgagtgt cccacgagca ccgccttctt cgcatcgctc tcgagcagcg
     5641 ccctgtccac cgtgctcgcg acttcgaaga ccagttgccc gaagccacgg aagtatccca
     5701 gggtccagtc aaggggcgtt aagggggcaa tatgcacttc ggagccggag atctcctgca
     5761 gcaagctcgc gaagttgcgg taactacccg gccaccaggt agccccgccg acgatcacta
     5821 ttggtgcgcc agacatgccc gagagtatag ccgccttcag gtgatgctcg cctggaggcc
     5881 gtcgatcacg aactggactg ctagcgcggc gagtaatacg ccgaggacac gtgagagtac
     5941 gtcggccccg gtctcaccga agagccgcat tacgcgcggg gcgaggagca gcgaactcaa
     6001 ggtcagaagc aggatcacga acaggaccgc catgaacagg gctacctggc cagcgctgct
     6061 gccgcccgta aagaggagga ccgtggtgat ggcccctggt cctgccagaa gcggtatggc
     6121 aaggggaaag actgaaatgt cctgctggta ggattccttc tcctgttcgc gcacgctcgt
     6181 ggagcgcatc cctgaaggcc gggcgaagac catgtcgagt gagaggagga agagcagtag
     6241 gccgccggcg atccggaaag ccggcagtcc gatgccgaga gcctcgagca gtacgtcgcc
     6301 agccagggcg aacaggaaga gtatgagggc gcctagcagc gtgcccctta tggctgacgc
     6361 gcgtttttgc tcctcggaga aaccgcgggt gagtgcggcg aagatggggg cgagacccag
     6421 cgggtccacg accacgagca gcgttacgaa agcgttgaat gcgaactcca tcggacgaaa
     6481 ctctacctca cagcgaagaa cctgtgtcgg ggatgacacg ccactctccc cgtaacgacc
     6541 gcgcccggtc catggtcttc ttcccgatcc ggcctctctt cgtctggtaa cgcccgcgca
     6601 gctcctccga gagtgccttt atcacctgcg atacatctgc cggcaggttc ccgatgcttt
     6661 ctggcctgaa cggtagtccc aataatccat agacgcgctc cagtacgtcc ctgacctcag
     6721 ccgtctcaga gaccagtacg agggcttcga ggcgggcgga ccggcgcgtg accctttgtg
     6781 ccagcccggc gtgcttgacg ccccacgggc cgccggagcg cacagagtaa gccccgggac
     6841 agaactcgcc ctcgacctcg ccgccctccg cttcgacccc gatacgccgc agggctgagg
     6901 cgatgagatc caggccctcc gagtaacgct cgtagaggcc gtgccgcaga gcctctacgg
     6961 gaaaggtgag ggagaacgac agagagcccc tgttcgccgc gacggccccg ccgccggagt
     7021 tccgcaccag cacggggaag ccgagtcctg aggccgcttc tgtggccgcc gcgaagccag
     7081 gcagacgcgt ctcctgcctg gtcgccccca cgtagcgcga ggctgtccac atcagggccg
     7141 tggggccgcg ttcgtcggcc gcgacctcct cgagcagagc ctgttgtagc ccgaaaccga
     7201 cttcgggggc gtcgaatggc ccggcgatga ggatttccac gcgttcactt gcgggcaacg
     7261 aaaagccccg tgtccttggt gatgtggatc tccgagtagg tcacgtctgg cctcctggtt
     7321 gggtgtctac cggcccctgg agggccgcca catctgtggt gctaagatag cagcccggat
     7381 gcagggtcgg atagtacgga ggaggatgct ccagcaggaa gccatgatcg agcgaacgcg
     7441 ccgcctctgt agggaggatg aacgcctcgc tgcggcgatg atatacggct ccttcgccca
     7501 gggtgagggg gatgggttct cggacatcga gttcatcctg tttttcaggg atgaggagct
     7561 ggagggcctc gaccaggagg agtgggtctc caggatctca cccgtcgggc tgtacttcgt
     7621 gaacgagtac gggaacggga cggcgatctt ccagaacctc gtcaggggtg agttccactt
     7681 cgaccgcgct tcggatatcg agaggatcgt cggcgaatcg atgagagacg cggactggct
     7741 tccctcgcta gaagacaccc tcatcctcga ccgcactgga gagttgggcc cacgcctgcg
     7801 agagatcgtc ggtccgccac cggagcgcga cacgccgggg cagatacggt tcgtctgcga
     7861 ctgcttcgtg aactggttcg tcttcggctc gaacctcttc gccaggggcg agcttgcgcg
     7921 atccctggat ctcctcggga tcgtacgcga ccgcctgtta cagatcgtca gagtgcagga
     7981 aaagtcgacg ttgcactggt tcaactccgc gaagtttctg gagacggaga tatcccagac
     8041 ctcctacgcc cgctacgcgg cctgcaccgc ccgccttgac agggacgagc ttcgccaggc
     8101 ttacctctct gcctgggagt gggggaggga gctgatggcg tccctcgcgg agagacacgg
     8161 cgtcgctccg ccgactgcac tcctggtgcg gctggaccgg cgctttgccg agaccttcag
     8221 cgctcctggc ctggaggatt catgaggtgg gacgagacac agagcccgtg gcggacgctc
     8281 ggttccagga acgtctacga gaacccctgg atctcggtcc gcgaggacag cgtgataagg
     8341 cctgacggcg agcctggcat ctacggcgtc gtccactaca agaacaccgc cgtcggcgtc
     8401 ctgcctgtgg aggaggatca cgtctacctc gtaggccagt accgctatcc cctggaacgc
     8461 tactcgtggg agatacctga gggcggctgt ccggaagacg aggaacccct gcgtgcggcc
     8521 cagcgcgagt tgagggagga gacaggcctg gaagccagga actggcggat gctcggagaa
     8581 gcttacctgt ctaattccgt cgctgacgag tatgctgtgt ggtttctcgc cacgggtctt
     8641 gtaccagggg agcggaagcc agaagggacc gaggtcatcg gcgtgcgatg cgtacccttg
     8701 cgcgaggccg ttgccatggc gatggacggc aggatcaccg acgccctcag caccctcgcc
     8761 ctcacgacct atgcactaaa gggttacgat ggcgcggccg gctagggtgg gccgctgcgt
     8821 atggggggtg cgatgaaggt ctgtgcgctc gccggcgggg taggtggcgc gaagcttgct
     8881 agcggtctgc aggacgtgct gctgccgggg gatctctcgg tggtagtgaa caccgccgac
     8941 gacttcggcc tgtgggggct gcacatctgc cccgacctcg acacggtcat gtacacgctg
     9001 gccgggatct ccaaccctga gaccggctgg ggcattgcag gtgagtcttt cgagactctg
     9061 aacatggtcg aaagctacgg tgaggagacc tggttcaagc tcggagacag ggacctcgca
     9121 acccacgtac tcagaacgtc ccgcatgctc tcaggggaga cgctcaccga ggttacggcc
     9181 ggactatccg gtgctcttgg cgtggagagc gtcgtgctgc cgatgagcga cgatcctgtc
     9241 tcgacggtgc tcgagacccc tcaaggccgg ctcgagttcc aggagtactt cgtgcgccgt
     9301 ggacagagag acgaggtgct agggatcgag ttgcgaggta tcgaggatgc caggcccacg
     9361 gagcgtgtcc ttgcgacggt ctcccgtgcc gacgccatcg tcttgtgtcc gtcgaacccg
     9421 gtcgtgagcg tgggcccgat cctggccctg cctgggatga tggaggccct ggccacctca
     9481 gacgccccga aggtggccgt cagcccgatc gtcggcggac gggcgctcaa gggtccggca
     9541 gacaggatgc tcgcctcgct cggccacgag gtctctgcca cgggcgtggc caggatgtac
     9601 gcaggcctgg ttgacgggat ggtcgtcgac cggaccgacg aggatgaacg ggccggcatc
     9661 gaggccctcg ggatgcgcgt cctggtaacg gagtcgatta tgcgcgacgc cgaagaccgg
     9721 gcgcgcctcg ggtcggagac gctagagttc ggcgcaggat tggtggcgcg atgagcacaa
     9781 tctccgctgt cgtacctgtc aaagaccttt ggggcaccaa gagtcgcctg gcgccgatcc
     9841 tcgatcccgg cgccagggca gggctcaccc tgtacatgat gggccgtgtc gtgcgtgcga
     9901 tcgcggaggc aggggtcgag gacgtctgcg tagttagccc cgactggatt gttctggaag
     9961 aggcaaagag acgcggcgca acaccgctcg tccaggagag tcgcgggctc aatcccgccc
    10021 tcgaagaggg acgccgcagg gcgctggggc tcggtgcctc gacgctactc gtgctccccg
    10081 cagaccttcc gctcctcgat gcgggtgacg tgcgagcggt actgcaggag gcgggggagg
    10141 ggccttcggc ggtcatatcc cctgacgggg ggcgttccgg cacgaacgcg ctcctgatcc
    10201 aaccccccga cgtcctgacc ttcgccttcg gtcccgacag cttcgaagca caccttgggg
    10261 ctgcccgcgg acgcagcctg gacgtcagga tctgcgagcg gccccacctc gccttcgatc
    10321 tcgacacggc gggagacctg gcccgtctcg gagagacagg agttgcgagc ccgtgagggg
    10381 tatcgaggtg cttggcgtcg agggcttccc cgaggtccgc cccggcgacg acctgaaccg
    10441 tatgatctct ggggcggtag cgaggaacct gcgtccccgc gacgtgctcg tggtaacgca
    10501 caagatcgtc agcaaggccg agggccgtct ggtcgacctt cgcacaatcg agccgtccac
    10561 gctcgcgaag ggttacgcga cgcgctacgg gaaggacccg cgccagatcg aggtcgtgtt
    10621 tcgagagagc cgccgcatcg tacgcatgga ccgcggcatc atcatctcgg agacccacca
    10681 cggtttcgtt tgcgccaacg cgggcgtaga cgcctcgaac gttcctgacg acgacgccgt
    10741 ctgcctgctc cccgtagacc ctgatgcctc cgcgaggagg ctccgtgacg cgctcgccga
    10801 agacccaggc gtcgaggtgg ccgtggtgat ctcggactcg ttcgggcgga cctggcgcca
    10861 tggaataacg gacgtcgcca tcggcctcgc cggcatggac cccgtcgctg attacagggg
    10921 gcagagggac ccgcacggct tccctatgga ggccagcgta ctcgccatag ccgacgagct
    10981 cgccgccgcc gcggagctgg taatgggcaa gaccgacggc atccctctgg ccatcgtgcg
    11041 ggggtattcg tactcgcccg catcgggcag cgccagagag ctcctcatgc cacctgagag
    11101 ggacatgttc aggtaggatg ctggcgtttc ttgacgccct cggaggttct ggggtacgat
    11161 caccccgtgc taacgcttct ctagacggcg gagaccgaac aggaggaccg caggacccgc
    11221 ggttctcgat cgctctgccg taccttcgca gcgtcccatt ccgaaggctg tcgtgcgccg
    11281 gtgtctgtgt gaatagggac gcttagacac ccttcctcct ggccgaacag gcgtgcgtat
    11341 cgagaaagcg ggtcttgaat ggacaacctg agagcctatc cgatctacct cggtatcagg
    11401 gggtcgttcg cgttcttctt cacgctttgg gccaccgtgg cggctgtcta caggatcgag
    11461 gtcgtgcatc tcgacccact gcggctggtg ttgctgggga cggcgctcga ggtcgcgatc
    11521 ttcctcttcg aggttccgac cggtgtgttt gccgacacct acggccggcg acggtcggtc
    11581 attacgggtt gcatactgat ggggtgcggt ttcgcgttag agggtgccat ccccgaattc
    11641 atagccgtgc tcgccgccca ggcggtgtgg ggtgtcgggt acacgttcat cagcggggcg
    11701 ctcgaggcct ggatcgcgga cgaagacgcc gaccgagacc tgggaagggt gtacctgcgc
    11761 ggagagcagg cagactactt cggctccttc gtcggcatcc ctgcgagcgt gcttctcggc
    11821 ctcgtcacgt tgaacctgcc gctcatcgtc ggcggcgtcc tcaccatcac gctaggcctc
    11881 gcgctcttcc tcacgatgcc cgagcgcaac ttccgaccct ccccgcgcga ggggcgctcc
    11941 tcgctacacc acgcggctac cacggcgc
//
LOCUS       DASTKM010000053         6410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1067043, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000053 DASTKM010000000
VERSION     DASTKM010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067043"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VFQ10_02110"
     CDS             complement(<1..818)
                     /locus_tag="VFQ10_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9964165.1"
                     /translation="MGLIPYKNEVYLDWTEEKNVEAMRAALKDVGSRLGRSYPAIIGG
                     RRVETDDEIVSVNPADPSQVVGRTASATEREADMALETATSTFETWSRTSPEARARIL
                     LRASAIMKRRRFELLAWEAYEGGKPWFEADAQVAEAIDFLEYYAREMLRLKDGVEIYS
                     VPGEESRYTYQPMGVGVIIAPWNFPTAILTGMSSAAIVAGNTIIMKPSEFTSVIGAVV
                     AEIFEEAGLPEGVLNFLPGYGSEVGDYLVEDARTRFISFTGSMKTGLRINELAA"
     gene            complement(828..1745)
                     /locus_tag="VFQ10_02115"
     CDS             complement(828..1745)
                     /locus_tag="VFQ10_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET9964166.1"
                     /translation="MGIVDKAIANSVPAIPRPIVKRISSRYIAGDTLHEALSTVRDLN
                     EQGCVATIDLLGESTESKADATATLKNYEKVVDALDECGLKGGISVKLTGLGLTLNQQ
                     LCRANLEEIVEYAKERGRFVRVDMEDSPYTESTVNMVRDVHGRHENIGAVFQAYMRRS
                     IEDVQTAIEHGFSVRLCKGIYDEPRSIAYKDFDTIRQNYILLLDELLTGGVYVGIATH
                     DEFLIWHALRLIHQLEVPEDRYEFQMLLGVDEELRSILVEAGHKLRVYVPFGEDWYEY
                     STRRLKENPKIAGYVARDVIGGITARVKR"
     gene            1923..3467
                     /locus_tag="VFQ10_02120"
     CDS             1923..3467
                     /locus_tag="VFQ10_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9964167.1"
                     /translation="MASIIGGHAPDGAPGGRLTSTNPARTSETVAEVSLGDAGTFVEA
                     CRAARGAQREWASVPAPIRSQVVKKIGRVVEKNKEALARLVTREIGKPYAESLGEVQE
                     IIDTCDFFTGEGRRLYGQTVPSEMPDKQLFTFRVPVGVAAIITAGNFPVAVPSWYLIP
                     ALTCGNAVVWKPAEYSPAIGDALAKLFMAGGLPDGVLNVVQAEGLAAFEGLEKALDER
                     LVDKVGFTGSSEVGKRIGELCGRHMQSPCLELGGKNPLVVMPDADLDLAVEGALFSGF
                     GTAGQRCTSLGTVIVQQSVHDEFVSRFSRAVEEAAVGDPTKDVLYGPMLSERFHERFL
                     GWLELIEPHHSIHGSSGMGRISADNPRDGFVGDPEAGIYDHPTIVDGVAMEDEIYRTE
                     TFGPIVGVATFSDFDEAIALSNAHGYGLSAAIYTTDATNALRFRERNGAGMLSVNNST
                     SGAEAHLPFGGNGKSGNGSRLSGIWVLDQFTRWQSMNWDYAGKLQKAQMDITDLPADL
                     DYTLPE"
     gene            3480..4628
                     /gene="solA"
                     /locus_tag="VFQ10_02125"
     CDS             3480..4628
                     /gene="solA"
                     /locus_tag="VFQ10_02125"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HET9964168.1"
                     /translation="MYDVIVAGLGGMGSSAAYHLAGRGERVLGLERHTLAHDKGSSHG
                     QSRIIRLAYSEDPAYVPLVLRAYELWERLERETDENLMTLTGGLMIGPPGTKFFEGSK
                     ASAEQYDLPYEVLDAAELKRRYPVLEPTPDTVALFEEKAGFLRPEASVKAHLDMAASL
                     GAELRFEEELLSWEPTQSGVLVETASGTYEAERLVVSAGAWAPKLLAHLGLPLEVTRQ
                     LLFWFDPKGGIEPFLPDRFPIFIWEPEDGNSFYAVPAHDGPRGGVKVAFFRADGKTAD
                     PETIDREVHDEEVEFIRSYLARYVPDLDGDFLYAKICMYTNTPDEHFVISAHPKYPQV
                     AIAAGFSGHGYKFCSVVGEILADLVTEGETPYPIDLFSSTRLVGTARG"
     gene            4795..5175
                     /locus_tag="VFQ10_02130"
     CDS             4795..5175
                     /locus_tag="VFQ10_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9964169.1"
                     /translation="MQNDLVKSESLSGIEVGRESEEMCLLARLFNGFANSTRLSILLL
                     LAQRGEMKVGELVDELGAPQPRVSDHLRCLAWCGYVQVRREGRNAFYAVADERVMQIL
                     ELGEELLQDNLEHVEACDDIEKGC"
     gene            5275..6387
                     /locus_tag="VFQ10_02135"
     CDS             5275..6387
                     /locus_tag="VFQ10_02135"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VLRF1 family aeRF1-type release factor"
                     /protein_id="HET9964170.1"
                     /translation="MAQAQDLKQTITSVAGRPGPVLSAYLSVNADIPENQGRAYLVRL
                     RDAMNDEGVPQGLQRRVREYTAEETHPGARTLAIFADEDNLFEVYRLQVDVPESFRWG
                     DPYVAPLTLVLDEYEPYGAAVLDAERFRYFVVSPLSTAHGEEEIRANGFREVDVHPSE
                     PYPRGGTDFEAASRRTEAHVHRFYKQLGELTRDVTFREGVQRLILAGPRERTAEFRRT
                     LPNELKNWVVAEEHVDLGLPEGELLDRLEAVRERAEHERQKNLLDEIRESGVRGLDET
                     VAALQEENRVYHLAALWELEAEIRWCANDQLAIRDATREECPFCGQKTRVRPLTDVLI
                     DLAAARGARLDFVRGEDEDSDTLRNEFGGIAGLTRF"
BASE COUNT         1215 a         2041 c         1966 g         1188 t
ORIGIN      
        1 ctggccgcca gctcgtttat cctgagtccc gtcttcatcg agccggtaaa ggagataaaa
       61 cgcgtccttg catcctctac cagatagtcc ccaacctccg agccgtagcc tggcaggaag
      121 ttcaagacgc cttcgggcag gcctgcctcc tcgaagatct cggccaccac cgcgccgatg
      181 acgctcgtga actctgaggg cttcatgatg atggtgtttc ccgcaacgat ggcagcgctc
      241 gacatcccag taaggatggc ggtagggaag ttccaggggg cgatgatgac cccgacgccc
      301 atcggctggt aggtgtagcg gctctcctcg ccggggacgg agtagatctc gaccccgtcc
      361 ttgagcctca gcatctcacg agcgtaatac tcgaggaaat ctatggcctc cgccacctgc
      421 gcgtcggcct cgaaccaggg cttgccgcct tcgtaggcct cccaggcgag cagctcgaac
      481 cttcgccgct tcattatcgc cgatgccctg agcagtatgc gcgcccgtgc ctccggcgag
      541 gtgcggctcc aggtctcgaa agtggaggtc gcagtctcca gggccatgtc cgcctcgcgc
      601 tcggttgcgc tggccgtgcg gcccaccacc tgcgagggat cggccggatt caccgacact
      661 atctcgtcgt ccgtctcgac gcgcctgccg ccgatgatcg cggggtagct cctgcccagc
      721 cgggagccga cgtctttcag ggcggcccgc atagcttcga cgttcttctc ctcggtccag
      781 tcgagataga cttcgttttt gtacggtatc agtcccatgt ctggctccta tctcttcacc
      841 ctggcggtta tgcctccgat cacgtccctc gcgacgtagc cggcgatctt ggggttctcc
      901 ttgagacgcc tggtcgagta ctcgtaccag tcctctccga aaggcacgta gaccctgagc
      961 ttgtgcccgg cctcgaccag gatgctcctt agttcctcgt ctaccccgag gagcatctgg
     1021 aactcgtatc ggtcctcggg gacttcgagc tggtggatga gcctgagggc gtgccagatc
     1081 aggaactcgt cgtgcgtcgc tatgccgacg tagacgccgc ctgttagcaa ctcgtcgagt
     1141 aggaggatgt agttctgcct gatcgtgtcg aagtctttgt aggctatgga cctcggctcg
     1201 tcatagatgc ccttgcaaag gcgcacggag aaaccgtgct cgatagccgt ctgcacatct
     1261 tcgatgctgc ggcgcatgta cgcctgaaag acggccccga tgttctcgtg gcgtccgtgc
     1321 acgtcgcgaa ccatgttcac ggtggattcc gtatacggcg agtcctccat gtctacccgt
     1381 acaaagcggc cccgttcttt ggcatactcg actatctcct ccaggttcgc cctgcagagc
     1441 tgctggttca aggtcagccc tagcccggtg agcttgacgg agataccccc cttcagaccg
     1501 cactcgtcga gtgcatcgac gaccttctcg tagttcttca gcgtcgccgt cgcgtcggcc
     1561 ttgctctcgg tgctctcacc caggaggtcg atggtggcga cgcagccctg ctcgtttagg
     1621 tctctgaccg tagagagcgc ctcatggagg gtatccccgg caatgtagcg gctggagatc
     1681 ctcttcacaa tgggccgcgg gatggctggc accgagttcg ctatggcttt gtccactatt
     1741 cccacgcctc aaaaccccac aaaacgacct ctattgcgcc agatacatcc ccgattttac
     1801 tctaaggagc gtcccgaggc tttggtgtag gatagtggca agacaggtga acgagagagg
     1861 tccggaggca gagaatgcgt accaccgacg cccttgaagg aacagcttca gagacccgaa
     1921 ccgtggcctc gataataggg ggacacgctc ccgacggcgc tccgggcggg cgcttgactt
     1981 cgacgaaccc ggcgcgtacg tccgagacgg tggccgaagt atcgctcggg gacgcaggga
     2041 cattcgtaga ggcgtgcagg gccgcgcgcg gggcgcagcg ggagtgggca tcagtgcctg
     2101 cccctatccg ctctcaggta gtaaagaaga tcgggcgcgt cgtcgagaaa aacaaagagg
     2161 cgctcgcgcg gctggtgacc cgggagatcg gcaagcctta cgcagagtct ctcggcgagg
     2221 tgcaggagat catagacacc tgcgacttct tcaccgggga gggccgtagg ctctacggcc
     2281 agaccgtccc ctctgagatg ccggacaaac agctcttcac cttccgcgtc cctgtcggcg
     2341 tcgcggccat catcacggcc ggcaacttcc ccgtcgcggt cccttcgtgg tacctgatac
     2401 ccgccctcac ctgcggcaac gccgtggtct ggaagcccgc cgaatactct cccgccatcg
     2461 gcgacgccct ggcaaagctc ttcatggccg gcggcctacc cgatggtgtc ctcaacgttg
     2521 tgcaggccga aggactggcg gccttcgaag ggttggagaa agccctcgac gagaggctcg
     2581 tcgacaaggt aggctttaca ggctcttccg aagtcgggaa gagaataggc gagctctgcg
     2641 gcaggcacat gcagtctccc tgcctggagc tcggaggcaa gaaccccctc gtagtcatgc
     2701 ccgacgcaga cctcgacctc gccgtcgaag gcgccctgtt ctcgggcttc ggcacggctg
     2761 gccagcgttg cacttctttg ggcaccgtga tcgtccaaca gtccgtccac gacgagtttg
     2821 tgtccaggtt ctcccgcgcc gtcgaggaag cagctgtagg ggaccccacc aaagacgtac
     2881 tctacggacc catgctcagc gagcgcttcc acgagcggtt tttgggctgg ctcgagctga
     2941 tcgagcccca tcacagcatc cacggttcga gcgggatggg caggatctcc gcggacaacc
     3001 ctcgcgatgg cttcgtcgga gatcccgagg ccggcatcta cgaccacccg accatagtag
     3061 acggcgttgc gatggaggat gagatctacc gcacagagac cttcggcccc atcgtcggcg
     3121 tcgccacttt ctcggacttc gacgaggcga tagccctctc caacgcacac ggctacggcc
     3181 tctcggccgc catctacacc accgacgcga cgaacgcgct acgcttcagg gagcgcaacg
     3241 gcgcgggaat gctctccgtc aacaactcca ccagcggcgc ggaggcccac ctgccctttg
     3301 ggggcaacgg gaagagcgga aacgggagcc gtctctcggg catctgggtt ctcgaccagt
     3361 tcacccgctg gcagtccatg aactgggact acgctggcaa gctccagaag gcccagatgg
     3421 acataaccga cctccccgcc gaccttgact acacactgcc tgagtaagag agccgtctga
     3481 tgtacgacgt gatcgtcgcc ggcctcggcg ggatggggag ttcggcggcc taccacctcg
     3541 ccgggcgcgg cgagcgcgtg ctcgggctgg aacgccatac cctcgcgcac gacaagggat
     3601 ccagccacgg gcaatccagg atcatccgcc tggcgtactc cgaggacccc gcctacgtgc
     3661 cgctggtcct tagggcctac gagctgtggg agcggcttga gagggagacg gacgagaacc
     3721 tgatgacgct cacaggagga ctcatgatcg ggcctccagg caccaaattc ttcgagggca
     3781 gcaaggcgag cgccgagcag tacgatctgc cctacgaggt cctcgacgcc gccgaactga
     3841 aaaggcgcta tcctgtcctc gagccaaccc ctgataccgt cgccctcttc gaggagaagg
     3901 cgggctttct caggccagag gcttccgtga aagcccacct cgacatggcc gcttctctgg
     3961 gcgcggaact ccgcttcgag gaggaactgc tgtcctggga accgacgcag tccggggtgc
     4021 tggtggagac ggcatcgggg acttacgagg cggagcggct ggtagtctcg gccggggcgt
     4081 gggcgccgaa gctgctcgct cacctcggac tcccgctcga ggttacacgc cagctcctgt
     4141 tctggttcga cccgaagggc ggcatcgaac cgttcctccc tgaccgcttc cccatcttca
     4201 tctgggaacc cgaggacggc aactcgttct acgccgtccc cgctcacgac ggacccagag
     4261 gcggggtcaa ggtcgcgttc ttcagggccg acggcaagac tgctgatcca gagacgatag
     4321 accgcgaggt ccacgacgag gaggtcgagt tcatcaggag ttatctcgcc aggtacgtgc
     4381 ccgacctcga cggtgacttc ctctacgcga agatctgcat gtacacgaac actcctgacg
     4441 agcacttcgt gatctccgcg caccccaagt acccccaggt cgccatcgcc gccggcttct
     4501 ccgggcacgg ctacaagttc tgcagcgttg tcggggagat cctggccgac ctagtcaccg
     4561 aaggcgagac cccatacccc atagacctct tctcctccac ccgcctggtt ggcaccgcac
     4621 gcgggtagcc ttggagatcc tggctggtaa acacttcagc tcttcgcgtg cgcgagtcct
     4681 ggccgacgtg ctgaccagca cgcgggaagg tctccttcct gcgtcccgct gtaataggct
     4741 tcacacgttt gaaccggcgg tgtatagtag gaaagtacgt ggaaggagtg cactgtgcag
     4801 aacgatttag tcaagtcaga atccttatcc ggtatcgaag ttggtcgcga gagcgaggag
     4861 atgtgcctcc ttgccaggtt gttcaatggt ttcgccaact cgaccagatt atcgatcttg
     4921 ctgcttctgg cgcaacgggg cgagatgaag gtcggagagc tggtagacga gctcggggcg
     4981 ccccagccga gggtctcgga ccacctgcgc tgtctggcct ggtgcggtta cgtacaggtg
     5041 cgacgggagg gcagaaacgc tttctacgcc gtggccgacg aaagggtcat gcagatactc
     5101 gagctaggcg aggaacttct ccaggacaac ctcgagcacg tcgaggcctg tgacgatata
     5161 gagaagggtt gctagactcc gctccagaac cggagagcgt gtttactcga ctcgaagggc
     5221 ggggattcta gtagggccag tcctcgtaag atatacgcaa gaggtgtttc aggcatggcc
     5281 caggctcagg atctcaagca gacgataaca tccgtggcag gcaggccggg accggttctc
     5341 tcggcttacc tgagcgtcaa cgccgacatc ccggagaacc agggaagggc gtacctggtc
     5401 cgactgcgtg acgccatgaa cgatgagggc gtcccccagg ggctgcagcg gcgggtcagg
     5461 gagtataccg ctgaggagac gcacccgggg gccaggacgc tcgccatctt cgcggacgaa
     5521 gataacctct tcgaggtcta ccggttacag gtcgacgttc ccgagtcatt ccgctgggga
     5581 gacccttacg tggccccgct gacgctcgta cttgatgagt acgaaccata cggggctgcg
     5641 gtcctcgacg ccgagaggtt caggtacttt gtggtctctc ccctgagtac cgcccatgga
     5701 gaagaggaga taagagccaa cggcttcagg gaggtcgacg tccatcccag cgagccttac
     5761 cccagaggcg ggacggactt cgaggctgcc agccgccgga cggaagccca cgttcacagg
     5821 ttctacaagc aactgggaga gctaaccagg gatgtcacct tccgtgaggg ggtgcaacgt
     5881 ctgatcctcg ccggccccag agagaggacc gccgagttcc gtcgcacctt gcccaacgag
     5941 cttaagaact gggtggtcgc cgaggaacat gtagacctag ggttacccga aggcgagctc
     6001 ctggaccggc tcgaggctgt cagggagcgc gccgagcacg agcgccagaa gaacctcctg
     6061 gacgagataa gggagagcgg cgttcgcggc ctcgacgaga ccgtcgcggc cctacaagag
     6121 gagaaccgtg tctaccacct cgccgctctc tgggaacttg aggccgagat ccgctggtgc
     6181 gctaacgacc agctcgccat acgcgatgcc acccgtgagg agtgtccatt ctgtggccaa
     6241 aagacccgcg tgcggcccct cacagacgtc ctcattgacc tcgccgccgc ccgcggcgcg
     6301 cgcctcgact tcgtgcgcgg cgaagacgag gattcggaca ccctgcgcaa cgagttcgga
     6361 ggcatagcag gactgactcg cttctgaatt agcaactcag catttcagct
//
LOCUS       DASTKM010000054        13864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1243356, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000054 DASTKM010000000
VERSION     DASTKM010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13864
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243356"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFQ10_02140"
     CDS             complement(<1..254)
                     /locus_tag="VFQ10_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET9964171.1"
                     /translation="MRRLALLSTTVLLLVLALPGLAFAQKGPSTADLSLAMDTMWVIV
                     AACLVLFMQAGFAMLEVGFSRMKNVGSVVAKILVNLAIAAL"
     gene            complement(437..2119)
                     /locus_tag="VFQ10_02145"
     CDS             complement(437..2119)
                     /locus_tag="VFQ10_02145"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9964172.1"
                     /translation="MILKDFSLILLQELDADCPLPGDAARLVRGLAVDEPVKGWISAD
                     EVAVTAREALDEDFLQEIASIGSPAVVWRLEGDVPAESVGRAAALGVGLFVLTPEVPL
                     RRLFEIFSQVEGLLLLSHGAGRTLLESAGGETTIEELAGKISGMLGRSVVVEDPVGRL
                     IASSGYEDHLRATLEEQGLRASKSTSIDETRRERRDRYARLPDGYLSVPVERWRVADG
                     ELFWTPIGAGAPAGYLWIDLEGGPLRPEDVVLLYWARRVLEAELGKERIRLETELGAR
                     GDFVDDLVSGHYGSVELLLQRARYLGADLTEGAVVMVVDIDDFARYLARRRPKEPAIQ
                     ELKRRLADAVRLQTRQLFSNFLLGPRSDNVILFLGYGEGEGPEELPEKTQLLAKGVQR
                     YVKGLLPDLTVSVGIGGYKQDPAQLPEAYSEARVALEIGHRIHGPSCVSTFGKTGTYK
                     LLFRVLQENPEELEAFYGETLEPVVHYDSRYGTELVQTLITYLGNDASTVRTAGDLVA
                     HRHTIRYRLDRVGELTGLDVDKSEDRERLTLGIKAMQLLGRVPERPSSFADR"
     gene            2330..5161
                     /gene="uvrA"
                     /locus_tag="VFQ10_02150"
     CDS             2330..5161
                     /gene="uvrA"
                     /locus_tag="VFQ10_02150"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9964173.1"
                     /translation="MVVRGAREHNLKDITVSMPRAVMVVVTGLSGSGKSSLAFDTIYA
                     EGQRRYVESLSAYARQFLGQMDKPDVDHIDGLSPAVSIDQKTTSNNPRSTVATVTEIY
                     DYLRLLYARAGRPHCHVCGFPVASSTPQQMVEKVLTLPEKTRFLVLAPVVRGRKGEYG
                     RMFKELAESGYARVRVDGELHELPVDLALDKNYKHDIEVVVDRLVIRDGIERRLTDSI
                     ETALNLAEGLVQIETVERDGAGESMLFSESFTCTNCGASIAEIQPRTFSFNSPHGACD
                     RCDGLGSRLEIDPALVVPNEDLSVNEGALVPWANSQTEYHASVLGALSEKYGIDLNLP
                     WRDLPEEHRHLILFGTDGERIYVSYRNRYNRRRQYMTRFDGVVGNLERRYAETDSEYR
                     REKIEEFMSHVPCPKCRGARLRPEALAVTVGGNNIYEFTELSVTGAQRFFDGVEFTTR
                     EWIIGERVVKEIRERLGFLVDVGLGYLTLSRSAGTLSGGEAQRIRLASQVGSGLVGVL
                     YVLDEPSIGLHQRDNRRLLTTLEKLRDLGNTLIVVEHDEETIRSADFIVDVGPGAGVH
                     GGEVIAAGSVGDIEAEERSVTGDFLAGRRRIEPPEERREPTGSIELLGAMEHNLKSVD
                     VEFPLGVFTCITGVSGSGKSTLVNEILYKALANAVSRGKHRPGRHAGLRGVQDVDKVI
                     DIDQSPIGRTPRSNPATYTKVFDHIRQLFASTPEAKIRGYKPGRFSFNVKGGRCEACR
                     GDGQIRIEMHFLPDVYVPCEVCKGRRYNNETLRATYKGKSISDVLDMTVEEACEFFAP
                     VPAISRRMDTLRDVGLGYVRLGQPATTLSGGEAQRVKLASELGKRATGKTVYILDEPT
                     TGLHFADVEKLLAILHRLVDAGNTVIVIEHNLDVIRSADHVIDLGPEGGDAGGEIIAT
                     GTPEEVAGVEESHTGRFLRGLMPEVVAAG"
     gene            complement(5218..5667)
                     /locus_tag="VFQ10_02155"
     CDS             complement(5218..5667)
                     /locus_tag="VFQ10_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964174.1"
                     /translation="MTERDDAQFTELFEVIGAYSRRGYYYQDKALQIIAGTYVFMFEQ
                     EEMPDVRPIVDDILGQYDYVFTTLERGNLDPLSVDAVVRVALYNEEYMEWGINRLGRI
                     LESLHRRSRADESYVDFVEDAAVVIRGLENIVAGSALEEIVEGTNGA"
     gene            5813..6703
                     /locus_tag="VFQ10_02160"
     CDS             5813..6703
                     /locus_tag="VFQ10_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964175.1"
                     /translation="MSAKNADSVVSPNQVMAAGLAATVASFVTSRFGVAGTLLGAALT
                     AMIITGGSAILRSYIEAASGRVRGMPTKLGARHGQQKGGGSGGTMPGRPDLRDNFVGR
                     MRAALDWFRHLPTLSRRSILIKGLLAAAVAFVLCMGVVWGAEKVIGNSLSCGIWAKCP
                     PGALPGIHPAGYGGTGAGSSISGGGMNTGGDERPGLVDRIRQNQNPTENRNPFENRNP
                     LENRNPLENRNPLENRNPFRDDRPALGNPGGGASSASPDPAPGAGGQESIPPNAGQES
                     ASPAAGGRKAQSTASPEAGR"
     gene            6787..8280
                     /locus_tag="VFQ10_02165"
     CDS             6787..8280
                     /locus_tag="VFQ10_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9964176.1"
                     /translation="MTSATYNVPRPALLLVLDGIRPDVLRAAIREGGAPNLGALAQRG
                     EAVWDAVSVFPSITPAATAAIATGEAPAKSGIVGHAWYDEYERRVVVYGAMTETVMAS
                     GPIRVFHNNVWRMNRDDLHAATLFEALHDRGFDGACVNFPVRRGPHTHPVRMKTIEGY
                     LMGGRYLGPSVQGPKEYFMGDLFYSRDTGFSGRRANGGVLRSVGINDDYAARVGAMLL
                     RERAVPFNLVYFFKGDSIAHHHGLSAQRQWLTTADEYVARLFSAGGGVDRVLEDYAVL
                     ALSDHGHAPLLPKSRYVDLRIIPGQKVSSGVKARFGNGTTIVVVPNGRSVLLYLRGDV
                     ELESVVERLVGRRGVDLAAWMEDGWAVVRRLGRELRFLPGSGVRDSFGRSWELEGDPR
                     ALDIDVSDGSLHYGEYPDALERLWGCLHSPRCGDVVLSAAPGYTFGEISGGSHTASDH
                     GSLHASDSNVFVLASGVPAPRRITDVAPTLLSHFGADLVAGDRVLQE"
     gene            8264..8995
                     /locus_tag="VFQ10_02170"
     CDS             8264..8995
                     /locus_tag="VFQ10_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964177.1"
                     /translation="MSSRNNGSEEPSGLRPRYRRAFGCVTWVYMGMLVFLAANFLLVL
                     PSRYLGSGELYGYLPPTLAYLGTLLILPGMVLAALLGARTFRTPRTRGTRVGAGVGAL
                     IGYSGFFGLAWLAMALGLNRRDQAFRTVVFPDLGGSLAFYAFVPLTLLAMGLVIFALY
                     TRGADFERRRRLVYAGAALAVLAGLGVVAADPDPLGVAGALISTLSGAIGGYVSGSGF
                     ARAGGDDMIPPGAEIQRREPRRKSR"
     gene            9034..10872
                     /gene="uvrC"
                     /locus_tag="VFQ10_02175"
     CDS             9034..10872
                     /gene="uvrC"
                     /locus_tag="VFQ10_02175"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET9964178.1"
                     /translation="MPENGFPDRSHLPTSPGVYIFKDAADTVLYVGKAKNVRARVASY
                     FNRSGDGRPKIAELRECVRQIDFIVTGSETEALVLEANLIKRHRPRFNASLKDDKSYP
                     YIVVTTGDEYPRVFATRAAHDPRHRYFGPFPSAGSVHSTLDVLNKTFPFRKCRGPEPG
                     RRSGVPCLNYHIGRSAAPCIGAISKEDYDGIIADVISFLEGRVDGLIREREAAMREAA
                     RDMDFERAAKLRDEISALSHVRDRQQATIASEDSFDAFGAYSEGETACMQVFAVREGQ
                     IVNRDSFLLDNYGESRAEDVSLQFVPQYYGTAAVPREVLVQSDDREDELHTLADHLSD
                     LRGTSVEVRRPRRGDKRRILEMAVHNAELGLEHERMLDEARRNRVASTLDGLREELAL
                     PRLPVRIECYDISNTMGTNSVASMVVFQGGRPAKSEYRRFRVRTVEGADDFASMAEVI
                     RRRLERLEADDEKFTPAPDLILLDGGKGQLSAVIPVLEEMKVGDELPDIPLRSLAKRE
                     EEIFEPGRPEPVILERNSPELHLLQRVRDEAHRFANAYHTKLRGRAMTASVLDDLPGV
                     GPERKKKILAHFGTPEAFLNASLEELESVPGLPGRVAREVHARVHR"
     gene            11104..12033
                     /gene="rapZ"
                     /locus_tag="VFQ10_02180"
     CDS             11104..12033
                     /gene="rapZ"
                     /locus_tag="VFQ10_02180"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET9964179.1"
                     /translation="MHPRRDGRRDVDEQATGSQASGKRRIVIITGLSGAGKSNALQAF
                     EDAGYFCIDNLPPRMIPAVMETTAQGNGGPDGVVLVVDMRGRRYFGEELERNLEAVDK
                     EEGWEHSILFIDSDDPTLIRRYKESRRPHPAARNGDVLEAIRRERRDLANVREMADLV
                     VDTSGYSALDTRLRFKKLAESFSTRMTVSLISFGFKHGTPLDVDTLLDVRFLPNPHYD
                     PELRPLTGHDAPVREAVLGSEDCQYFLDKTCELFSFLIPRYAAEGKTYFTIGVGCTGG
                     RHRSVAITEEIAGRLRQENADMDLFIRHRDTEK"
     gene            12356..13006
                     /locus_tag="VFQ10_02185"
     CDS             12356..13006
                     /locus_tag="VFQ10_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964180.1"
                     /translation="MKSIPAGKYVSDEFRPAMSFRLNEGWRTGPAADFSYGLFMEKYN
                     RLTLSRVSESEPSYLEFLVVPKVYKVVSSYEAKEEPAPEDMVSWLQNNPNLDTENPEP
                     MMVGGVEGAQFDAVASRIPQEYLSGGYHGVNTYHVRHGGNIYDDAPCLPLFQTLPGYG
                     RDSTYELCKMYKVRFIVLEDVNGKTVTIAVLAPTVKFDETWIKARRVLNTVEWKGT"
     gene            13302..13460
                     /locus_tag="VFQ10_02190"
     CDS             13302..13460
                     /locus_tag="VFQ10_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964181.1"
                     /translation="MDHIQKHYYRSHESINPTRIVPKGPILDFTEAHDRESFSDQIPQ
                     GNGYTRYA"
     gene            13457..>13864
                     /locus_tag="VFQ10_02195"
     CDS             13457..>13864
                     /locus_tag="VFQ10_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HET9964182.1"
                     /translation="MNRVEDTRVVAFGGGTGLPVLLRGLRDRVGDLTAVVTVADDGGS
                     SGRLRQELGVAPPGDVRNCLVALAGRRRLAEVFEYRFEAPGDLNDHSVGNIIIAALAD
                     MAGGFCEGVEQAARFLRIKGRVLPAATESLTLVV"
BASE COUNT         2637 a         4357 c         4385 g         2485 t
ORIGIN      
        1 agggcggcga tggcgaggtt taccaggatc ttcgccacca cgctccccac gttcttcatc
       61 cgggagaagc cgacctcgag catagcgaat cccgcctgca tgaaaaggac cagacacgcc
      121 gctacgatca cccacatcgt gtccatcgcc agcgagagat ccgctgtcga tggacccttc
      181 tgcgcgaacg ccagcccggg tagcgcgagc accaacaaca agaccgtggt cgatagtaac
      241 gcgagtctcc gcaaaagctt cctccatatc ttcagggtat gttctctcgg aacatagtct
      301 aggcactggc cacacaaggg tcattagccg aatgtacaaa gatggcgccc ccgcccgatg
      361 ctctcattct ggcgaaagta caaggaggcg gggagcacgc tgacaagccg tctgacgaag
      421 ccagcgtgct tacatgctat ctatccgcga aactcgatgg tctttcgggg acgcggccca
      481 ggagttgcat tgcctttatt ccgagggtga gacgttcgcg gtcctcgctc ttgtcgacgt
      541 cgaggcctgt aagctcgccg acgcggtcga ggcggtagcg gatcgtgtgg cggtgggcga
      601 cgagatcgcc cgctgtcctg acggtcgagg cgtcgttgcc caggtaggtg atcagggtct
      661 gaaccagctc cgttccgtag cgggagtcgt agtggacgac gggctccagg gtctcgccgt
      721 agaatgcttc tagctcctcg ggattttcct ggagcaccct gaagaggagt ttgtatgttc
      781 ctgtctttcc gaaggtcgag acacaggagg gtccgtggat gcggtgcccg atctccagcg
      841 ctaccctggc ctcggagtag gcctcgggga gttgggcagg atcctgcttg tagcccccga
      901 tcccgacgga gaccgtaagg tcgggtagca ggcccttgac gtagcgctgt acacctttgg
      961 ccagcagttg cgtcttctcc ggcaactcct cgggaccttc gccttcccca tatcccagga
     1021 agaggatgac gttgtccgaa cgcgggccga ggaggaagtt cgagaagagc tgacgggtct
     1081 gcaggcgtac agcatccgcc aggcgtcgct tgagctcctg gatggctggc tccttcggtc
     1141 tgcggcgtgc gagatagcgg gcgaagtcgt ctatgtcgac gaccatgacc acggctcctt
     1201 cggtaaggtc ggcgcctagg tatcttgcgc gttgcaggag gagctcgacc gagccgtaat
     1261 ggccgctcac caggtcgtcc acgaagtctc ccctggcacc cagttccgtc tccaggcgta
     1321 tgcgctcctt gccgagttcg gcttccagga cgcgcctggc ccagtaaagt agcaccacgt
     1381 cttcgggcct cagggggccg ccctccagat cgatccagag gtaccccgca ggggcgccgg
     1441 ctccgatggg ggtccagaac agctcgccgt cagcgacccg ccagcgttcg acgggcacgg
     1501 agaggtaccc atcaggtagc ctggcgtagc ggtcgcggcg ctcccgacga gtctcgtcga
     1561 tactcgtact cttcgaggcg cgtagaccct gctcttcgag cgtcgcgcgc agatgatcct
     1621 cgtacccgga gctggcgatc aggcggccca cagggtcctc cacgaccacg gaacgtccta
     1681 gcatccctga gatcttgcct gccagctctt cgatcgtggt ctcaccgccg gcgctctcca
     1741 agagcgttcg gcctgcgccg tgggagagca gcagtagccc ttcgacctgg gaaaagatct
     1801 cgaagagccg ccttaagggt acttcaggag tgagcacgaa aaggccaacc ccgagcgcgg
     1861 cggcccgtcc gaccgattcc gccggcacat cgccctctag ccgccagacg acggcgggtg
     1921 agccgatgga ggcgatctcc tgcaggaaat cctcgtccag ggcctcccgg gctgttacgg
     1981 ccacttcgtc ggctgagatc caacctttga ccggctcgtc gacggcgaga ccgcgcacca
     2041 gcctggcggc gtcacctggc agcgggcagt ccgcgtccaa ctcttgtaga aggatcaggg
     2101 agaagtcctt caagatcact gcgggctccg ttcgcgacgt ggtgtaagac cggcgtttat
     2161 cattttggcc ccgaccgccg aaaatcgcaa acgcggccac ggaaatgggt cttcggagtg
     2221 ctagaatata gccaaaagca aactgcttct ttggcctccc aacagggggc cgtatacttg
     2281 cctgaggcgt cccgaggaag aggggtgaga agagatcgcg gaagaccaga tcgtcgtgcg
     2341 gggggcgcga gaacacaacc tcaaggacat aacggtctcc atgccgcggg ccgtgatggt
     2401 ggtggtgacc gggctctccg gtagcggcaa gtcttctctg gcattcgata ccatctacgc
     2461 agaggggcaa cgccgctacg tggagagcct ctccgcctac gccaggcagt tcctcggcca
     2521 gatggacaag cccgacgtcg accacatcga cggcctctca ccggcggtct ccatcgacca
     2581 gaagaccacg tccaacaacc cccgctccac ggtcgccaca gtgacggaga tctacgacta
     2641 cctgcggctg ctctacgccc gcgcagggag gccgcactgc cacgtctgcg gcttccccgt
     2701 cgcctcctcg acgccccaac agatggtcga gaaggtactt actctccccg agaagacgcg
     2761 ctttttggtg ctcgcgcccg tggtgcgggg gcgcaagggc gagtacggga ggatgttcaa
     2821 agagctggcc gaaagcggct acgcccgtgt cagggtcgac ggcgagctgc acgagctgcc
     2881 cgtcgacctc gcgctggaca agaactacaa gcacgacata gaggtagtcg tcgacaggct
     2941 ggtgatccgt gatggcatag agcgccgcct cacggactct atcgagacag ccctgaacct
     3001 ggccgaaggc ctggtacaga tcgagaccgt cgagcgagac ggcgcgggcg agtcgatgct
     3061 cttctcggag agcttcacgt gtacgaactg cggggcctcc atcgcggaga tacagccgcg
     3121 cacgttctcg tttaacagcc cccacggggc ctgcgaccgc tgcgacgggc tgggcagccg
     3181 gctggagata gaccccgcgc tcgtcgtgcc gaacgaggat ctgagcgtga acgagggggc
     3241 cctcgtgcca tgggcgaact cgcagaccga ataccacgca agcgtgctcg gcgccctgtc
     3301 ggagaagtac gggatcgacc tgaacctccc gtggcgcgac ctgccggagg agcacaggca
     3361 cctcatcctc ttcggcaccg acggcgagcg catctacgtc tcttaccgca accgctacaa
     3421 ccgccgcagg cagtacatga cccgcttcga tggcgtcgtc ggcaacctgg agcggcgcta
     3481 cgccgagacc gattcggagt acaggcggga gaagatcgaa gagttcatga gccacgtccc
     3541 ctgccccaag tgcaggggcg ccaggctcag gcccgaagcc ctggctgtga ccgtcggcgg
     3601 caacaacatc tacgagttca cagagctttc cgttacaggc gcgcagcgtt tcttcgacgg
     3661 cgtggagttc accactcggg agtggatcat cggcgagcgg gtggtcaaag agatcaggga
     3721 gcgcctcggt ttcctcgtcg acgttgggct cggatacctc accctgagcc gttccgcggg
     3781 gacgctctcg ggcggtgaag cccagaggat aaggctcgct tctcaggtag gaagcggcct
     3841 cgtcggggtc ctgtacgtgc tcgatgagcc ctccatcggg ttgcaccagc gagataacag
     3901 gcgtctcctc accacgctcg agaagctaag ggacctcggg aataccctca tcgtcgtcga
     3961 gcacgacgaa gagacgatcc gttcggccga cttcatcgta gatgttggtc caggcgcggg
     4021 ggtgcacggg ggtgaggtca tcgcggccgg cagcgtcggg gacatcgagg ccgaggagcg
     4081 gtccgtaacg ggcgatttcc tggccgggcg gcggaggatc gagccgcccg aggagcgacg
     4141 agagccgacg ggttccatag agttgctggg ggcgatggag cacaacctga aaagcgtgga
     4201 cgtggagttt ccgctcgggg ttttcacctg tattacgggc gtctcgggat cagggaagag
     4261 cacgctggtc aacgagatcc tctacaaggc cctagccaac gccgtcagcc gcggtaagca
     4321 tcgtcctgga aggcacgcgg gactcagggg agtccaggac gtcgacaagg tcatagacat
     4381 agaccagagc cctatcggga ggacaccccg ctccaacccg gcgacctaca ccaaggtatt
     4441 cgaccacatt cgccagctct tcgcttcgac gcctgaggcc aagatcaggg gctacaagcc
     4501 aggacgcttc tctttcaacg taaagggagg ccggtgcgag gcgtgccgcg gagacggcca
     4561 gatccggata gagatgcatt tccttcctga cgtctacgtc ccctgcgagg tctgcaaggg
     4621 ccgccgctat aacaacgaga ccctgcgggc cacctacaag ggcaagtcaa tctccgacgt
     4681 cctcgacatg accgtcgagg aggcctgcga gttcttcgcc cccgtacccg cgatctcacg
     4741 ccgcatggat accctgcgtg acgtcggctt gggctacgtc aggctcgggc agccggccac
     4801 cacgctctcc ggtggcgaag cccaaagagt caagctggcc agcgagctgg gcaagagggc
     4861 gacgggcaag accgtctaca tcctggatga gcctaccacc gggcttcact tcgcggacgt
     4921 cgagaagtta ctcgccatcc tgcacaggct cgtggacgcg gggaacaccg tcatagtcat
     4981 cgagcacaac ctcgatgtga tccggtccgc agaccacgtc atcgacctcg gtcccgaggg
     5041 cggtgacgct ggcggcgaga tcatcgcgac aggcaccccc gaagaggtag ccggcgtcga
     5101 ggaatcacac acgggccgct tcctgcgcgg cctcatgccc gaggtcgtgg ccgcgggcta
     5161 gtcaaaggct catgcagtcg gaccccgacc gagggcggtc cttctgatct tggtgctcta
     5221 ggctccgttc gtcccttcga cgatctcctc gagggccgaa ccggccacta tgttctccag
     5281 gccacggatc acgaccgctg cgtcctcgac gaagtcgacg tagctctcgt cggcgcgcga
     5341 gcggcggtgg aggctctcca gtatgcgccc caggcggttt atgccccact ccatgtactc
     5401 ctcgttgtag agtgcgaccc gcaccacggc gtcaacgctc aagggatcca agttgccgcg
     5461 ttccagggtg gtgaacacgt agtcgtactg cccgaggatg tcgtctacga tgggacggac
     5521 gtctggcatc tcttcctgct cgaacataaa gacgtaggtt ccagcgatga tctggagtgc
     5581 cttgtcctga taatagtagc cccttcggga gtaggctcct atcacctcga aaagctccgt
     5641 aaactgcgca tcatcccgct ccgtcattct ccaccctccg ctgatcgctc tgtggaaatt
     5701 ctacccctgc tgcgaccttt gccgccatgt ggatcggcca cgagaacttc ttcacgaaga
     5761 agtctctctc ggactttgaa acgacgcgga taggctgata gattcactcc ccgtgagtgc
     5821 caagaacgca gattcagtag taagcccgaa ccaggtcatg gcggcggggc tagccgctac
     5881 ggtggcgtct ttcgtcacct cccgcttcgg cgtcgctggc acgctgctgg gggccgccct
     5941 gaccgccatg atcataaccg gcgggtcggc gatcctcagg tcgtacatag aggccgcctc
     6001 gggccgcgtt cgcgggatgc caacgaaact aggcgcgcgc cacgggcagc agaaaggcgg
     6061 cggttctggt ggcaccatgc cgggacgccc cgacctgagg gataacttcg ttggcaggat
     6121 gcgggccgcc ctcgactggt tcaggcacct gccgacacta agccggcgtt cgatcctcat
     6181 aaagggactc ttggccgctg cagtggcgtt tgtgctctgc atgggcgtag tgtggggggc
     6241 cgagaaggtt atcggtaaca gtctctcttg cggtatctgg gcaaagtgtc cgcccggcgc
     6301 ccttcccggt atccaccccg ccggatacgg cggcaccggt gccggatctt ctatctccgg
     6361 cggtggcatg aataccggag gggacgaaag gccaggcctc gtcgaccgca tacgccagaa
     6421 ccagaacccg actgagaacc ggaacccatt tgagaaccgg aacccgttgg agaatcggaa
     6481 cccgttggag aaccggaacc cgttggagaa ccggaacccg ttcagagacg accgtcccgc
     6541 cttgggcaat ccggggggtg gtgcatcatc agcctccccc gaccccgcgc cgggagcagg
     6601 aggccaggaa tccatcccac cgaacgcggg ccaggaatct gcctcgccgg ctgccggagg
     6661 gcggaaggcg caatccaccg cgtcaccgga agccggccgg taacttcgtt cgggcaggcc
     6721 ctctgtcgcg tagcgcggtg cgtggttcgc tccgaagtcg gcgtgaagag ataaacttag
     6781 tcggctttga ccagtgcgac gtacaatgtc cccaggcctg ctctgctctt ggtgctcgac
     6841 ggcatcagac ccgatgtgct acgggcagcc atccgggagg ggggtgcccc gaaccttggc
     6901 gccctggccc agaggggaga ggcagtctgg gacgcggtct cggtcttccc tagtattacc
     6961 ccggcggcga cggccgcgat agcgacggga gaggctcccg caaagagcgg catcgtcggg
     7021 cacgcctggt acgatgagta cgagcggcgg gtggtcgtct acggtgccat gacggagacc
     7081 gtgatggcga gcggtcccat aagagtcttc cacaacaacg tctggcggat gaaccgcgac
     7141 gacctgcacg ccgcgaccct cttcgaggcc ctgcacgacc gcggtttcga cggggcgtgc
     7201 gtcaactttc ccgtcaggcg cggtccgcac acccatccgg tccgcatgaa gaccatagag
     7261 ggttacctga tgggcggacg ctacctcgga ccctccgtgc agggcccgaa ggaatacttt
     7321 atgggcgatc tgttctacag ccgcgatacc ggtttcagcg gacgtagggc caacggcggc
     7381 gtcctccgct ccgttggcat caacgatgac tacgcggcca gggtaggggc gatgttgctc
     7441 agggagcgtg cggtgccgtt caacctcgtc tacttcttca agggcgactc catagcccac
     7501 caccacggcc tctcggcgca gcgtcaatgg ctgactacgg ccgacgagta cgttgccagg
     7561 ctcttttcgg ccgggggcgg ggtcgaccgt gtgctcgaag actacgccgt actagcactc
     7621 tcggatcacg gccacgcgcc actcctcccc aagagccgct acgtcgacct gcgcatcatc
     7681 ccaggccaga aggtctcctc aggggtgaaa gcccgtttcg gcaacggcac cactatcgtc
     7741 gtggtcccga acggacgttc ggttctcctt tatcttcgtg gggacgtcga actggaatcg
     7801 gtggtggaga gactagtcgg gcgccgcggc gtggatctcg ctgcctggat ggaagacggg
     7861 tgggcggtcg tccgccggct cgggcgcgaa ctacgctttt tgccaggctc gggagtgcgt
     7921 gattcgttcg ggcggtcctg ggagctcgaa ggcgacccgc gcgccctcga catcgacgtc
     7981 tccgatggat ctttgcacta cggtgagtac ccggacgccc tggagcggtt gtggggctgc
     8041 ctgcactcgc cgaggtgcgg ggacgtggtg ctctcggcgg cgccagggta taccttcggc
     8101 gagatctccg gcgggtctca cacggcgagc gatcacggct cgctccacgc cagcgactct
     8161 aacgtgttcg tgctcgcgag cggcgtgccg gcgccgcgca ggatcacgga cgtggccccg
     8221 acgctcctct cacactttgg cgccgatctc gtcgccgggg accgtgtcct ccaggaataa
     8281 cggctccgag gagccctctg gactcaggcc gcgttacagg cgggcgttcg gatgtgttac
     8341 ctgggtgtac atggggatgc tggttttcct cgcggccaac ttcctcctcg tactgccgtc
     8401 cagatacctc ggtagcgggg agctttacgg ttatcttccc cccacgctcg cgtacctagg
     8461 aaccctcctg atcctacctg gaatggtgct cgcagcactt ctcggggccc gtacgttccg
     8521 gacaccgcgc acgcgcggga cccgcgtcgg ggccggggta ggcgccctga tcggctacag
     8581 tggctttttc ggcctggcat ggctcgctat ggcactcggg ctaaatcggc gagaccaggc
     8641 gttcaggacg gttgtcttcc ccgatctcgg agggagcctc gccttctacg ccttcgttcc
     8701 actgacgctc cttgcgatgg gcctcgtcat tttcgccctc tataccagag gggcggactt
     8761 cgagcgacgg cgccggctcg tctatgccgg ggcggcgctg gcggtgcttg cagggcttgg
     8821 cgtcgtggcg gcggatccag atccgctcgg tgtagcaggg gccttgatct cgaccttatc
     8881 aggagccatc ggcggctatg tgagcggatc tggtttcgcc agggccggcg gggacgacat
     8941 gatcccacca ggggcagaga tccaacgccg cgagccccgc cgcaagtctc gctgatccag
     9001 gaaacataac ccttcgcggt ataatcccac tcgatgcctg agaacggatt ccccgacaga
     9061 tcacaccttc ccacttctcc cggcgtctac atcttcaagg acgcggcaga cacggtccta
     9121 tacgtgggca aggccaagaa cgtgcgcgcc agggtggcca gctacttcaa ccgctcgggc
     9181 gacgggcgtc cgaagatagc ggaactcagg gagtgtgtca ggcagataga tttcatcgtt
     9241 acgggcagcg agaccgaggc tcttgtactc gaagcgaacc tgataaagcg ccacaggcca
     9301 cgcttcaatg cctcgctcaa ggacgacaag agctacccct acatagtggt caccaccgga
     9361 gacgagtacc ccagggtctt cgccacgcgc gcggcccacg acccgcgcca ccgctacttc
     9421 ggacctttcc cgagcgccgg cagcgtccac tcgacgctgg acgtgctgaa caagactttc
     9481 cctttccgca agtgccgggg tcctgagccg gggcgccgca gtggggtgcc ttgtctcaac
     9541 taccacatcg gacgttcggc cgcgccgtgc atcggggcca tctccaagga ggactacgat
     9601 gggatcatcg cggacgtcat ctcctttctg gaggggcggg tagacggttt gatccgcgag
     9661 cgcgaggccg ccatgcgcga ggccgccagg gatatggact tcgagcgggc ggcgaagctg
     9721 cgcgacgaga tctccgccct ctcccacgtc cgcgaccgcc agcaggcgac catcgcttct
     9781 gaggactcgt tcgacgcctt cggcgcctac tccgagggcg agactgcctg catgcaggtc
     9841 ttcgccgtgc gcgagggcca gatcgtgaac cgcgactctt tcctcctcga caactacggt
     9901 gagtcacggg ccgaggacgt ctcgctccag ttcgttcccc agtactacgg cacggccgca
     9961 gttccacgcg aggttctggt ccagtccgac gaccgcgagg acgagctgca tacgctagcc
    10021 gatcacctct cggacctgag gggaacgagc gtagaggtac gccgccccag acggggggac
    10081 aaacgccgaa ttctggagat ggccgtacac aacgcggagc tcgggttgga gcacgagcgg
    10141 atgctggatg aagccaggcg caacagggtc gcctctacgc ttgacgggtt gcgcgaggag
    10201 ctcgcgctac ctcggctgcc ggttcggatc gagtgctacg acatctcgaa caccatgggg
    10261 accaactccg tggcctccat ggtcgtgttc cagggtggtc gtccggcgaa gagcgagtac
    10321 aggcgattca gggtcaggac ggtcgaggga gccgacgact tcgccagcat ggccgaggtc
    10381 atccgccgcc gactcgagcg cctcgaggcc gacgacgaga agttcacccc tgctcccgat
    10441 ctgatcctgc ttgacggcgg taaggggcag ctctccgctg tcatccccgt cctcgaagag
    10501 atgaaggtcg gcgacgaact acctgacata cccctgcgct cgctcgccaa gcgcgaagag
    10561 gagatcttcg agccgggtcg gcccgagccg gtgatcctgg agcgcaactc ccccgaactg
    10621 cacctcctcc agcgcgtcag ggacgaggcc caccgcttcg ccaacgccta ccacaccaag
    10681 ctccgtggca gagccatgac cgcctctgtc ctggatgacc tcccaggcgt cggccccgag
    10741 cgcaagaaga agatactggc ccacttcggc accccggaag cattcctgaa cgcctccctc
    10801 gaagaactcg agtccgtccc gggactcccc ggaagagtcg cccgcgaagt acacgcgagg
    10861 gtacacaggt agcagggatc aggtttgaac cgttgctgtg gagtgttgcg ttcgtcgctg
    10921 tgtcgtatca aggattagcc ataaggtcga gagtggttgt cggtgggcat actcgtggtg
    10981 ggtgctcgtg cctgattgct gagacctgtc gcctgatgcc tttccgttaa atgtcgctta
    11041 aacatgcgaa tcggcgttgc aagccggtat cgaggatgct aatctcgact tccgagatgg
    11101 cttgtgcatc ccaggaggga cgggcgacgc gacgtggatg agcaggccac cggctcccag
    11161 gcctcgggca aacgcaggat cgtcatcatc acagggcttt cgggcgcagg caagagcaat
    11221 gcccttcagg ctttcgagga cgctggttac ttctgcatag ataacctccc gccgcgtatg
    11281 atcccggccg tcatggagac tacggcgcag ggcaacggcg ggcccgacgg ggtggtcctc
    11341 gtggtagaca tgcgcggacg ccggtacttc ggcgaggagc tggaacgcaa tctggaggcc
    11401 gtggataagg aggagggctg ggagcacagt atcctgttca tcgactccga cgaccccacc
    11461 ctgatcaggc gctacaagga gagccgtagg ccccaccctg cagcccgaaa cggtgacgtc
    11521 ctcgaggcca tacgccgcga gaggcgggat ctcgcgaacg tgcgggagat ggccgacctc
    11581 gtagtcgaca cctcgggcta ctccgcgcta gacacccgcc tgcgattcaa gaagctcgcg
    11641 gagtctttct ccaccagaat gaccgtcagc ctcatctctt tcggcttcaa gcacggtacc
    11701 cccctcgatg tagacacgct actcgacgtc cgtttcctcc ccaaccccca ctacgacccc
    11761 gagctcaggc cgctcacagg ccacgacgcg cccgtgcgcg aggcggtcct cggctctgaa
    11821 gactgccagt atttccttga taaaacctgc gagctcttct ccttcctcat cccccgctac
    11881 gccgcagagg gcaagaccta cttcaccatc ggcgtcggct gcaccggtgg aaggcaccgc
    11941 tccgtagcca ttaccgaaga gatcgcaggg cgcctcaggc aggagaatgc cgatatggac
    12001 ctctttatca ggcacagaga cacggagaag tagcaccccc tggtacgata agaggtatcc
    12061 aaccttttct gacgctttgg cgctgatacg caagcagctg tgggtgtgag aagagcagac
    12121 ttcttgcggg tcggcgcagg tgaacgagat ggtaaaagtc tcgcaggcct aggagagtac
    12181 gatataggag atacatgagg cgacgggtga ggagcagtag caagttatgt atcgcccagt
    12241 agctgggaag ccacaccttg gtgtggggtt gttgatgttg cttatacttg tactgagcac
    12301 ctgtggtgtg gaggctcatg ctcgcaagat accggagagc tcaggcggga gatcaatgaa
    12361 gagcattccc gccggtaagt acgtctcaga tgaatttaga ccggctatgt cgttcagact
    12421 gaacgagggc tggcgaaccg gccccgccgc cgacttctcc tacggactct ttatggagaa
    12481 gtacaatcgc ctcaccctca gcagggtctc ggagtcggag ccctcctatt tggagttcct
    12541 ggttgtaccg aaggtttaca aggtcgtgag ctcctacgag gcaaaggagg agcctgcgcc
    12601 cgaggacatg gtctcctggt tgcagaacaa ccctaacctg gacacagaga atccggagcc
    12661 aatgatggtt gggggcgtgg agggtgcgca attcgacgcg gtcgcttccc gcataccgca
    12721 agagtatctc tccgggggtt accatggcgt caacacctac cacgtacgcc atggcggtaa
    12781 catctacgat gacgcccctt gcctgccgct cttccaaaca ttacctggct acggaagaga
    12841 cagcacttac gaattatgca agatgtacaa ggtccggttc atcgtccttg aggacgtcaa
    12901 cggtaagacg gtgactatcg ccgttcttgc gccgacggtc aagttcgacg aaacctggat
    12961 caaagcgcgg cgggtgctca acactgtgga gtggaaaggc acctaatctc gaaccaagga
    13021 gactattcag ctgatgtacg gcgggcgcga caccctcacg cggtgatctc gcgcctcggg
    13081 aggcgcaccg aggcaaccct tgctcccgag gcggcgcctc aggcgtgatc tgcacgacgg
    13141 tctaggcgcc caactagccg ggttgaacgt ctaggtcagg gagggctccg cctcaagtct
    13201 ttcccactct caccgagcgc gagatcccaa atctcatggc acaatggcga gcggcatcac
    13261 gccccgtaca gcggaaaacc tgtaacctac gatcgggagg tatggaccac atccagaagc
    13321 actactacag gagccatgag agcatcaacc cgacccgcat agtgccgaag ggcccgatcc
    13381 tcgacttcac cgaggcgcac gaccgcgagt ccttctccga ccagatccca cagggaaatg
    13441 ggtatactcg atatgcgtga atagagtcga ggatacaagg gttgtcgcat tcggaggcgg
    13501 cacggggcta cccgtgctgc tgagaggctt gagagacagg gttggggacc ttacggctgt
    13561 cgtcacggtc gccgacgatg ggggctccag cgggcgactg cgccaggagc ttggggtcgc
    13621 gcctccgggc gacgtgagga actgcctggt tgcgctagcg ggacggcgca ggcttgccga
    13681 ggtcttcgaa taccgtttcg aggcgccggg ggacctgaac gaccacagcg tgggcaacat
    13741 cattatcgca gcgctcgctg acatggccgg cgggttctgt gagggtgttg agcaggcggc
    13801 ccgattcttg aggatcaaag gccgagtcct cccagcggcg acggagagcc tgacgctcgt
    13861 cgtc
//
LOCUS       DASTKM010000055         5098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_715013, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000055 DASTKM010000000
VERSION     DASTKM010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715013"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFQ10_02200"
     CDS             complement(<1..194)
                     /locus_tag="VFQ10_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9964183.1"
                     /translation="MDPLVTTDWLAAHLDDEKVRVVDIRGYVDKTDLGGGRQKAEYLA
                     AREEYDEAHIPGAVYVDWTS"
     gene            complement(198..1751)
                     /locus_tag="VFQ10_02205"
     CDS             complement(198..1751)
                     /locus_tag="VFQ10_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET9964184.1"
                     /translation="MADIEKLEVPGPLARLGEAFEEAGHELYLVGGVVRRALLRGSDR
                     DTATEAQINLQDADAATDAHPARIKALLRPHAENLWTVGERFGTIAATIGEYEVQITT
                     YRTDQYTQGSRHPEVRFGDNLQEDLARRDFTINAVAADALTGEIVDPFEGRKDLEKGV
                     IRAVGDPLERMREDPLRMLRAVRFETTLSKPEKPFAMTQDLEGAIRNNAGWLESISAE
                     RIREEFEKILVSENVASGLRTLVRLGLMPYVVPEFMETVDVEQEAEFHHKDVFEHTLI
                     VVSSVEADPILRKAAFFHDIGKPRTLVYEHRCNYCGAKSIRKSAEEGECEHCGGRTVP
                     KKIHFYGHENVGAAIARRAMQRLAYPKDDADAVAHLVANHMRPYGYAASRDPWSDAAV
                     RRFIRDTYLARGDRVLADVDMLLRLARADITGSAPRRRRIAEESWRSLRSRVDQVRAE
                     DAVEKLESPLSGNDLMGQFGLGPGPWIKALKDYLQNEVVEGRLGKDDKERARGLAEAY
                     AREHDILEE"
     gene            complement(1826..2194)
                     /locus_tag="VFQ10_02210"
     CDS             complement(1826..2194)
                     /locus_tag="VFQ10_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HET9964185.1"
                     /translation="MDSREERADTDFEASIAEHAQGYSVVSVRGEMDLHSAPRFQYAI
                     ERAVENEDVGAVVVDLGDVAFMDSTALSALMRSKDTLGQRGISLRLAAPSRAVERIFS
                     VTGFQDYFDVFPSREAAIPV"
     gene            2290..2877
                     /locus_tag="VFQ10_02215"
     CDS             2290..2877
                     /locus_tag="VFQ10_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9964186.1"
                     /translation="MEGRSTERVAVFVDGANLYHSIKNYYKGILDYARLLSAAVGDRK
                     LLRATFYIVEKQEADEGQGTTSARSFVYNLNKFGYKVRSKPLMVHETLTPEGERTISH
                     KGDWDIGVVVDMVRLAGHADTYVLVSGDGDYVEAVEYLQSEKGIRVEVISAAQCTSQA
                     LLDVCDAHTDLADIPDLFRRPGPPKAVGDQHSAIS"
     gene            complement(2914..3807)
                     /gene="murB"
                     /locus_tag="VFQ10_02220"
     CDS             complement(2914..3807)
                     /gene="murB"
                     /locus_tag="VFQ10_02220"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET9964187.1"
                     /translation="MHANTLHLLFPSAKFDEPLKRYTAWKIGGPADALLEPGSVDELI
                     EATEKAAEHGVPVTILGGGTNVLVRDGGIRGLTIRLAKSLTNVEVEGTRVTADAGVLY
                     PVLANNTAGRGLAGLEFATGIPGTVGGAVYMNAGAYGSETTEVLDWAEVFRDHEVVRM
                     ESTELDLSYRHSALHENPDWVVLRAGYTLAPGEPLVLKARIKEFRALRMNGSPNRPSC
                     GSTFKRPKGDFPGRVIEAAGLKGTRVGRIEVSPVHANYLVNLGDGTAQDALELIELVR
                     QQVSEKLGIELEPEVRVIGEP"
     gene            3864..4895
                     /locus_tag="VFQ10_02225"
     CDS             3864..4895
                     /locus_tag="VFQ10_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET9964188.1"
                     /translation="MKAMALAAGKGTRLFPLTGEIPKPMAPVVNTPIIEHIFDLLAGH
                     GIEEVYVNVHYLADALLEVYGEESRVNGMAVHLEREDELLGTAGGVKRLADRFDDTFV
                     VVSGDALTDVNIKELVEFHREKGALATIALRRVYDTSEFGVVEIDEEGNIQGFQEKPS
                     PEEAISTLANTGIYVFEPRALDYIPEGVFFDFAEDVFPKLLEHGERFVGYQEDFYWSD
                     IGTLAAYRQAQYDVLSGKVGVKIPGEKRGEGLWVGEDAQIHSSAEIDGHVLIGKDAVV
                     GRGVKLLGDVTVGSDCWIRPNVTIKRSILLPGASVGGGAYLEDCIVGHHYDVRPQETI
                     RGGALIRRA"
     gene            complement(4907..>5098)
                     /locus_tag="VFQ10_02230"
     CDS             complement(4907..>5098)
                     /locus_tag="VFQ10_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9964189.1"
                     /translation="SPGRVFPRTALLRQVWGEEEYIDERTVNVYVQRLRNKLGDDPTD
                     PKLIETVRGFGYRLVRPTA"
BASE COUNT          911 a         1590 c         1590 g         1007 t
ORIGIN      
        1 tcactggtcc agtcaacgta gacggcgcct gggatgtggg cctcgtcgta ctcctcgcgg
       61 gcagcgaggt actcggcttt ctggcgaccg ccgccgaggt ccgtcttatc tacgtaaccc
      121 ctgatatcca cgacacgcac cttctcgtca tcgagatgtg cggcgagcca gtcggtagtg
      181 acgagggggt ccatcttcta ctcctccagg atgtcgtgct cgcgggcgta ggcttcggcg
      241 agtccgcggg ctctctcttt gtcatctttg ccgaggcgcc cttctactac ttcgttctgc
      301 aggtagtctt ttagagcctt gatccaaggc cctggcccca ggccgaactg ccccatgagg
      361 tcgttgccgc tcagggggct ctcaagcttc tcgacggcat cctcggccct tacctggtcc
      421 accctgctcc tgaggctgcg ccaggactct tcggcgatgc gcctccgccg cggcgcgctc
      481 cccgtgatgt cggccctggc gagcctgagg agcatgtcca cgtccgcgag cactctgtcg
      541 ccgcgcgcga ggtacgtgtc cctgataaag cgcctgaccg cagcgtcgct ccacgggtcc
      601 ctgcttgcgg cgtacccgta aggacgcatg tggttggcga cgagatgggc gacggcgtct
      661 gcgtcgtcct tggggtaggc gaggcgttgc atggcccggc gcgctatggc agccccgacg
      721 ttctcgtggc cgtagaagtg gatcttcttg gggacagtgc gccctccgca atgctcgcac
      781 tccccctcct cggcgctctt ccgtatgctc ttcgccccgc agtagttgca gcggtgttcg
      841 tagacgagcg tccttggctt cccgatatcg tgaaagaaag ctgccttgcg caggatggga
      901 tcggcctcga cgctggagac cacgatcagg gtgtgctcga agacgtcctt gtggtggaac
      961 tcggcctcct gctccacgtc gaccgtctcc atgaactcgg gcacaacgta gggcatgagc
     1021 cccagacgaa ccagcgtcct cagaccagac gccacgttct ccgagaccag gatcttctcg
     1081 aactcctccc ttatgcgctc ggcggagatg ctctccagcc agccagcgtt gttacggatc
     1141 gccccttcca gatcctgcgt catggcgaaa ggcttctccg gcttcgacag cgtcgtctcg
     1201 aagcggacgg cgcgcagcat tctcaggggg tcctcgcgca ttctttcaag aggatcgcct
     1261 acggcccgga tcacaccctt ctccagatct ttgcgtccct cgaaggggtc tacgatctca
     1321 cccgtgagcg cgtcggcagc gacggcattg atcgtgaagt ccctgcgtgc cagatcctcc
     1381 tgcaggttat ctccgaagcg tacctcgggg tgcctgctcc cctgggtgta ctggtcggtc
     1441 ctgtaggtgg tgatctgaac ctcatactct cctatggtgg ccgctatggt cccgaagcgc
     1501 tcacccaccg tccacaggtt ctcggcatga ggccgcagta atgcctttat cctcgcagga
     1561 tgcgcatccg tcgccgcgtc cgcatcctgc aggttgatct gtgcttctgt cgcggtgtcc
     1621 ctatcagacc cgcggagcaa cgccctccgc acgactccgc ccaccaggta gagctcgtgc
     1681 ccggcctcct cgaacgcctc accgagacgc gcgagaggtc ctggaacctc cagtttctct
     1741 atatcggcca tcacaatgac cagattctag tacgcaacgg gctgcggatc aggaccacgc
     1801 ctcacaggca catgggctcg agaggctaaa ccgggatggc ggcttcgcgg gatggaaaga
     1861 cgtcgaagta gtcctggaag cctgtcacgg agaagattcg ttcgacggct ctagaggggg
     1921 cggcgaggcg aagagagatc cctctttggc cgagggtgtc tttggagcgc atgagggccg
     1981 agagggcagt ggagtccata aacgcaacgt cgcccaggtc cacgacgact gcgcccacgt
     2041 cctcgttctc gacggcccgt tctatagcgt actgaaacct cggggcgcta tggaggtcca
     2101 tctcgccccg caccgagaca acggagtatc cttgggcatg ctcggctatg ctggcctcga
     2161 agtccgtgtc cgctcgctcc tctctagagt cgatgcaaag tggtcaatgg atattagcgt
     2221 agttcgtgtg cctggtaaac cgcctccttt gtacgggcat gcaatgtttt ctgctaatat
     2281 tcgggcgtga tggaagggag aagtacagaa cgggtggccg tcttcgtcga cggggccaac
     2341 ctctaccact cgatcaagaa ctactacaaa ggcatcctcg actacgccag gctcctctcg
     2401 gcggccgtcg gagacaggaa gttacttcgg gccactttct acatcgtcga gaagcaggag
     2461 gccgatgagg gacagggtac gacctcggcg cgctccttcg tctacaacct caacaagttc
     2521 ggctacaagg tacgctccaa gccgctcatg gtacacgaga ccctgacccc ggaaggggag
     2581 aggactatct cacacaaggg cgactgggac ataggcgtcg tggtggacat ggtgcgcctc
     2641 gcagggcacg ccgataccta cgtcctcgtc tctggcgacg gagactacgt ggaggccgtg
     2701 gagtacctcc agagcgaaaa aggcatccgc gtcgaggtca taagcgcggc ccagtgcacc
     2761 tcccaggccc tcctcgacgt ctgcgacgcc cacacagacc tggcagacat accggatctc
     2821 tttaggcggc ccgggccgcc taaagcggtc ggcgatcagc attcagcgat cagctgaatt
     2881 gcatgtcagg gcgcgtgctg aaatgctgac tggctacggc tcacctatta cccttacttc
     2941 cggctccagc tcgatgccca gtttctcgct gacctgctgg cggacgagtt cgatcagttc
     3001 cagggcatct tgggccgtac cgtccccgag gtttaccaag tagttggcgt gcaccgggga
     3061 gacctcgatc cggcccacac gcgtgccttt caggccggcc gcctctatta cgcggcctgg
     3121 gaaatctccc ttggggcgct tgaacgtgga gccacagctt ggcctgttag gtgagccgtt
     3181 catacgcagc gccctgaact ccttgatgcg ggccttcagt actagcggct cgccaggggc
     3241 gagcgtatag ccggcccgca gaacgaccca gtctgggttc tcgtgcaggg cgctgtgacg
     3301 gtatgacagg tccagttctg tactctccat cctgactact tcgtggtccc tgaacacctc
     3361 ggcccagtcg agaacctcgg ttgtctcgga gccgtaggcg ccggcgttca tgtagactgc
     3421 gccgccgact gtgcctggaa tgcctgttgc gaactccaga ccggcgaggc cgcggcctgc
     3481 cgtgttgttg gcgagcacgg ggtagagtac cccggcgtcg gcggtaacgc gcgtcccttc
     3541 gacctcgacg ttcgtcaggg atttggcgag ccggatcgtg agccccctga tgccgccgtc
     3601 cctcaccagc acgttcgtac ccccgccgag gatggtcacc gggacgccgt gttcggcggc
     3661 cttctcggtg gcttctatca gctcatctac gctcccgggc tcgagcaggg cgtctgcggg
     3721 acccccgatc ttccaggccg tgtagcgctt caagggttcg tcgaacttgg ccgaggggaa
     3781 gagcaggtgt agagtgttgg catgcatgag agggaatata gagtaccgag gaatgctttg
     3841 cagaacgggc gagggagaga tatatgaaag cgatggctct agcggcgggc aagggaacca
     3901 ggctcttccc gcttaccggt gagatcccca aacctatggc tccggtggtg aacacaccga
     3961 tcatcgagca catcttcgac ctccttgccg gccacggcat cgaggaggtc tacgtcaatg
     4021 tccactacct ggcagacgcg cttctggaag tctatggcga agagtccagg gtgaacggca
     4081 tggccgtaca tctggaaagg gaggacgagc tgctgggcac ggcggggggc gtgaagcgtt
     4141 tggccgacag gttcgacgac accttcgtcg tcgtctcggg cgacgcgctg acggacgtga
     4201 acataaagga gctggtggag tttcataggg aaaagggcgc actggccacg attgccctgc
     4261 ggcgcgtcta cgacacctcc gagttcgggg tggtcgagat cgacgaggag ggcaacatcc
     4321 agggattcca ggagaagcca agccctgagg aggccataag caccctggcg aacacaggca
     4381 tctacgtctt cgagccacgg gccctcgact acatcccaga aggcgtcttc ttcgacttcg
     4441 ccgaagacgt attccccaag ttactggagc acggtgagcg gttcgtaggg tatcaggagg
     4501 acttttactg gtcggacata gggacgctcg cagcgtaccg ccaggcccag tacgacgtgc
     4561 tctcaggcaa ggtcggggtc aagatccccg gcgagaagcg tggtgagggg ctgtgggtcg
     4621 gagaggacgc ccagatacac tcctccgcgg agatagatgg ccacgtcctg atcggaaaag
     4681 acgctgtggt cgggcggggc gtcaagctcc tcggggacgt aaccgtgggg agcgactgct
     4741 ggatcaggcc taacgtcacg atcaaacgca gcatcctgct cccaggtgcg agcgtgggcg
     4801 gcggcgctta cctggaggac tgcatcgtcg gccaccacta cgacgtgcgt ccccaggaga
     4861 ccataagggg cggcgctctg atacggcgcg cgtaggggcg ccgccgctag gccgtcggcc
     4921 tcaccaggcg atagccgaaa ccgcgtacgg tttctatcag cttggggtct gtcgggtcgt
     4981 cgccgagctt gttgcgtagg cgctggacgt agacgttgac cgtgcgctcg tctatgtatt
     5041 cctcctcgcc ccacacctgc ctgaggagcg ccgtgcgcgg gaagaccctt cccgggct
//
LOCUS       DASTKM010000056         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_539032, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000056 DASTKM010000000
VERSION     DASTKM010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539032"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      <1..140
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            147..422
                     /locus_tag="VFQ10_02235"
     CDS             147..422
                     /locus_tag="VFQ10_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964190.1"
                     /translation="MPEQRPFWELIMFGRQHGPRAQREEKVLHYIIHRVDEDADLHDV
                     LQEPYVRRNCSQGEIDEIIMNPELLHHCREHLEHVFASGELDPKRQR"
     gene            440..2389
                     /locus_tag="VFQ10_02240"
     CDS             440..2389
                     /locus_tag="VFQ10_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HET9964191.1"
                     /translation="MSGATAAPKTQLARLKLWLLEKRIEEVQGPEAREGEEQHPWWQV
                     VCLTGVDYFSTLGYIPAIAAVAAGALSPIATLLIVLLTLFGMLPMYRRVAEESPHGQG
                     SIAMLERLLSFWKGKLFVLCLLGFVATAWIVTITLSAADAAVHIAENPLVSSFLHDRE
                     VLITLVLLAVLGAIFLKGFKEAIGIAIFIVGAYLILNLLVVGVGFYEIATHPQNVVRW
                     QDALFTNYGNPLLMVGVSLLVFPQLALGLSGFETGVGLMPLVRGDEEDDPQRLEGRIR
                     NTRRMLTVAALIMSFYLITTSFVTTLLIPARELAPGGSANGRALAYVAHNYLGEALGT
                     TYDVSTILILAFAGASAMAGLLNIVPRYLPRYGMAPEWARAIRPLVLVYTAVAVVVTI
                     IFSADVNAQAGAYATGVLAMMSSAAFAVTLSAWRGGSRFPAIAFGLVTAVFMYALVAN
                     EIKRPDGIVISLFFIGAIVATSLISRVYRSLELRKERIELDETALRFVEEASHEGEIH
                     IVANRRQAGDEAEYDNKEKEQREDNHIPPNVPIIFLEVDVEDASEFEDVLKVRGVEVS
                     GHKVLRAVSSVVPNAIAALLLHLRDTTGKTPHCYFGWTEGNPIVYLFRFLLFGEGDTA
                     PVTHEVLREAEPDGTRRPAVHVGGR"
BASE COUNT          489 a          845 c          845 g          497 t
ORIGIN      
        1 ccggggggcg ggggacccac acggacctct aaggaggtcc gcggggtgaa tcgctaggcc
       61 ggcgaagcca gccagcgtag ggcttttctc tttcggcccg aacccgacag ctaaccccgc
      121 aggcgcagaa gaaggaggta gtaagcgtgc ccgaacagcg acccttctgg gaactaatca
      181 tgttcggcag gcagcacggc cctcgcgctc agcgcgagga gaaggtcctc cactacatca
      241 tccaccgcgt ggacgaggat gccgaccttc acgacgtact ccaggagccc tacgtgcgcc
      301 gcaactgctc ccaaggcgag atcgacgaga tcatcatgaa tcccgagctc ctccaccatt
      361 gcagggagca cctggagcac gtgttcgcct ccggggagtt agatccgaag cggcagcgct
      421 agggatcgga ggataggcga tgtccggggc cacggccgca ccgaagaccc agcttgcccg
      481 gctaaagctc tggctgctcg agaagcgcat cgaggaggta caggggcccg aggccaggga
      541 aggcgaggag cagcacccgt ggtggcaggt ggtgtgcctg actggcgtcg attacttctc
      601 caccttgggc tacatacctg ccatcgcggc ggtcgcggcc ggagcgctct cgcctatcgc
      661 cacccttctc atagtgctct tgaccctctt cgggatgcta ccgatgtaca ggcgggtggc
      721 cgaggagagc ccccacgggc aaggctcgat cgcgatgctc gagaggttgc tctcattctg
      781 gaagggcaag ctgttcgtgc tctgcctgct ggggttcgtg gccacagcct ggatagtcac
      841 gatcaccctc tccgcggcgg acgccgccgt gcacatcgcc gagaaccctc tggtatcctc
      901 tttcttgcac gaccgggagg tcctcatcac gttggtgctc ctggcggtcc ttggcgcgat
      961 cttcctcaag gggttcaagg aggccatcgg catcgcgatc ttcatcgtcg gggcctacct
     1021 catcttgaac ctcctcgtcg tgggcgtcgg tttctacgag atcgcaacgc acccacagaa
     1081 cgtagtccgc tggcaggatg cgctgtttac gaactacggc aacccactct tgatggtagg
     1141 tgtctcgcta ctcgtgttcc cgcagctagc gcttgggctc tcgggctttg agaccggtgt
     1201 gggtttgatg ccgctcgttc gcggcgacga ggaagacgat ccccagcgcc tcgaggggcg
     1261 catccgcaac acacgcagga tgctcacggt tgcggccctg atcatgagct tctacctgat
     1321 taccaccagc tttgtgacga cgctgctgat ccctgcccgg gagctggcac cgggaggcag
     1381 cgccaacggc cgggcgcttg cctacgtggc gcacaactac ctcggcgagg ctttgggcac
     1441 gacctacgac gtgagcacga ttctcatcct ggcgttcgcc ggagcctctg cgatggccgg
     1501 gctgttgaac atcgtgcccc gctatttgcc ccgatacggg atggctcctg agtgggcgcg
     1561 ggcaatacgg cccctcgtgc tcgtatacac ggccgtggcc gtcgtggtca cgatcatctt
     1621 ctctgccgac gtgaacgccc aggcaggcgc ctacgccacg ggtgtgctgg ctatgatgtc
     1681 ctcggccgcc ttcgccgtta cgctctcggc ttggcgcgga ggctccaggt tcccggcgat
     1741 cgccttcggg ctggtcacgg ccgtattcat gtacgcactc gtggccaacg agataaagcg
     1801 tcccgatggg atagtgatct ccctgttctt catcggggcg atcgtcgcca cctcgctcat
     1861 ctcgcgggtc taccgctcgc tggagctcag gaaggagcgc atagaactcg acgagacggc
     1921 gctgaggttc gtcgaggagg cgagccacga aggcgagatc cacatcgtcg ccaaccggcg
     1981 ccaggcgggc gatgaggccg aatacgacaa caaggagaaa gagcagcgcg aagacaacca
     2041 cattccaccc aacgtcccca tcatattcct tgaggtggac gtggaggatg cttcggagtt
     2101 cgaggacgtg ctgaaggtga ggggggtgga ggtcagcggc cacaaggtcc tgagggcggt
     2161 gagttcggtc gtgcccaacg ccatcgccgc cctcttgctt cacctgcgag acacgactgg
     2221 caagacgccg cactgctact ttgggtggac ggagggcaac cctatcgtgt acctgttccg
     2281 cttcctgctc ttcggggagg gggataccgc tcccgtcacc cacgaggtct tgcgcgaagc
     2341 cgagccggac ggcacgagac gtccggccgt ccacgtaggc ggcagatgac tcgaaccttc
     2401 aaaaaccccc tcacccgaac ttcggagaat tctccttcca cgcactccgg ggaatacagg
     2461 agcgtagaag agcagaagtt catcctcgct gtcccgtctc ttatggtgcc ttatagtgca
     2521 tgcgtgggaa gaccgcgcat gaccaggagg gaatttttcg ccctaggagg cgccggagct
     2581 gcggccgccg ggctgtcgct ggccgaagcg atgagcggtg ggagagacga gcccaacctg
     2641 ctcgtcatca ttaccgacga tcagccttac tacacc
//
LOCUS       DASTKM010000057         3691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_11255, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000057 DASTKM010000000
VERSION     DASTKM010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11255"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFQ10_02245"
     CDS             complement(<1..345)
                     /locus_tag="VFQ10_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964192.1"
                     /translation="MAGNVRAYLRFAVGIIRGEVPYPAEVRGRPLDLLHEQLASRDRE
                     ISRLRARLAAGDSDDTIGGIPPEHFIWVFGVARTGSSWLGAMMGDLDDHAIWYEPYVG
                     DVFGYAYYMRAGE"
     gene            725..2281
                     /locus_tag="VFQ10_02250"
     CDS             725..2281
                     /locus_tag="VFQ10_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HET9964193.1"
                     /translation="MFVSDSRKVVFVHIQKTGGLTVDRLLNERIPDLRRIVARHGFAS
                     LGMDELEDWDEYFKFAFVRNPWDRLVSWYSMVTTMPREGNELWQYVHDNSSTFEEFIR
                     NCTDEVEIKEGVHYSFAYNQLDYVTDDHGNLLVDFIGRLENFDEDIQEVFRRIGFELE
                     VVPHKNRSGHQHYSTFYTPDTELIVRERFKRDIEYFGYEFESPRKPGALFVCGCDRSG
                     TTALTDYLNRHPKILVCQESSETTQQGEITLDPSTFERMLDPLPKEPGAPHNDGDRLI
                     KPPAELLANRDPSRLQWMGASNSDYLMRMESVAGNNPGARFIVVYRPIEEVAESWEPE
                     DADDRQNSNDGFGRAVKTWNRGLQGTRRFIRDSLIPRVLIIDYHDFLYRTETVAPLIS
                     RFLELEFDETVKAELSDEVPRSERVRSREKRSLIQEHANRAAEAWILDRIEKQWAKPA
                     LYTQRTSKSALATSLEEVEARTWRLQEKVRELERDRERRRRTFKQLQSSKTWKLLDRI
                     SSLRTRIAPR"
     gene            complement(2299..3378)
                     /locus_tag="VFQ10_02255"
     CDS             complement(2299..3378)
                     /locus_tag="VFQ10_02255"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9964194.1"
                     /translation="MFKINRAFGVGRDAKAFLNMQGGLTRGAVPNPKHHEEQAQRLRK
                     VVTRQRKALHRKDRQIEEQAAVLEEQAAALEPLRERRQREIKLRKELKQKSLEIFHLK
                     NELDAMKVLVENVRNVPSAEQDVGALPDFVIIGAQKGGTSFLYYLLTRHPLVEPAARK
                     ELHFFDQPEHFDNGAEWYRRCFPRLSSKDGQRSITGEATPYYMFDPPVAERMAEVVPQ
                     ARLIALLRNPADRAYSHYQMQVKRGTEPRSFEEAIEQQHSSYVSRGIYVDQLLRWFEF
                     FSKEQMLILKSEDFFERPVETLKGVLNFLDLPDWQPEASELQQRRHTGTYRQKMDPAT
                     RRRLEAYFEPYNQRLYECLDVDFGW"
BASE COUNT          795 a         1081 c         1053 g          762 t
ORIGIN      
        1 ctccccggcg cgcatgtaat aagcgtagcc gaacacgtct cccacgtagg gttcgtacca
       61 tatagcgtgg tcgtccagat ctcccatcat ggcgccgagc cagctactgc cggtcctcgc
      121 gacgccgaag acccagatga agtgctccgg agggataccg ccgatggtat catcgctgtc
      181 tcctgcggcg aggcgtgccc ttagccggga gatctcccgg tctctggagg cgagctgctc
      241 gtgcaggagg tccagtggtc tgccgcgtac ttcggcagga tagggcactt cgccacggat
      301 gatccctacc gcgaatctca aatatgccct cacgttgccg gccacatcca tctcccccgc
      361 gtccgtgcgc gaatctcggc actattcttc ccggtcttcg tccgcatccc atatactagc
      421 ccgtccggtt cgagccagcc gggccgaacg cgcaggacct gcggcgtaga cactatattg
      481 ccctttgctt ccgcagcgaa caagccggca caacgatctg caccctccgc accagaagac
      541 gttcagagag gcctcgtaat ctgtggttcg ccctccacca cgcgttttgt atccgcggat
      601 accgtactgt ccacatattt atcgggcagc ggggcctggt atcctcggct cacggttgaa
      661 agagcattgc tttgcaaagt agactaaacg gcggagaaga ctctagaggg aggattgggt
      721 aagagtgttc gtctccgata gccgtaaggt ggttttcgtc cacattcaga agacgggcgg
      781 actcactgtg gacaggttat tgaatgagcg tatccctgat ctacgtcgca ttgtggcgag
      841 gcacgggttt gcaagcctgg ggatggatga gctagaagat tgggacgaat acttcaagtt
      901 cgccttcgtg agaaatccgt gggatcgatt ggtctcctgg tacagcatgg taacgacgat
      961 gccaagagaa ggcaacgaac tatggcagta cgtgcacgat aacagctcga ccttcgaaga
     1021 gttcatccgc aactgcacgg acgaagtaga gataaaggaa ggcgtccact attcgttcgc
     1081 gtacaatcaa ctagattacg tgacggacga ccatggcaac ttgctcgttg acttcatagg
     1141 ccgactggag aacttcgatg aggatatcca ggaggtcttc cgtaggatag gcttcgagtt
     1201 agaggtagtc ccgcacaaga atcgctccgg tcaccagcac tacagtacgt tttacactcc
     1261 cgataccgag ttgatcgtaa gagagagatt caagagagat atcgagtact tcggctacga
     1321 gttcgaaagc ccaagaaaac caggagctct cttcgtctgt ggttgcgaca ggagcggcac
     1381 cacggccctc acagactacc tgaaccgaca tccgaagata ctcgtctgcc aggaaagctc
     1441 cgagacgaca cagcaggggg agatcacact ggatccctct acgttcgaga ggatgctcga
     1501 ccctctgccg aaggagccag gggcgccaca caacgatggg gaccgtctca tcaagccacc
     1561 agcggagctc ctggcgaaca gggatccgtc caggctgcag tggatgggcg ccagcaactc
     1621 cgactacctg atgcgtatgg agtcggtagc agggaacaac cccggggcgc gcttcatagt
     1681 cgtgtaccgt cccatcgaag aggtcgccga gtcgtgggag cctgaagatg ccgacgaccg
     1741 ccagaatagc aacgacggtt tcggacgtgc ggtgaaaacc tggaacagag gcttgcaagg
     1801 cactaggagg ttcataagag acagcctgat tccgagggta ctgataatcg actaccatga
     1861 cttcctctac cggaccgaga ccgtcgcgcc cctgatctca cgtttcctcg agctggagtt
     1921 cgacgagacc gtaaaggcag agctctcaga tgaagttccg cggtcagaga gggtgaggtc
     1981 cagggagaag cgatccctga tacaggagca cgccaaccgc gccgccgaag cgtggattct
     2041 cgaccgcatc gaaaaacaat gggcaaagcc agcactgtac acccagagaa cctcgaagtc
     2101 cgcgctcgca acctccctgg aggaggtgga agccaggacc tggcgacttc aggagaaggt
     2161 cagggagttg gagcgcgaca gggaacgcag acgccggaca ttcaaacagc ttcagagctc
     2221 aaagacctgg aaactactag acaggatcag cagcctccgg accagaatag cccccaggta
     2281 agtgcgctcc gaacgacgct accacccgaa atcaacgtcg aggcactcat agagccgctg
     2341 gttgtacggc tcgaagtagg cctccagccg ccgtctggtg gccgggtcca tcttttgtct
     2401 gtaggtgcct gtgtgtcgtc tctgttgaag ttccgaagct tcaggctgcc agtctggtag
     2461 gtcgaggaaa ttcaggacgc ccttcaatgt ctcgacgggg cgctcgaaga aatcctcgct
     2521 cttgagtatg agcatctgct ctttgctaaa gaactcgaac cagcgcagaa gctggtccac
     2581 gtagatgccc ctggacacgt atgaggaatg ctgctgctct atggcttctt cgaaggacct
     2641 gggttctgtt ccccttttca cctgcatctg atagtgggag taggccctgt cggccgggtt
     2701 cctgagcagc gctatcagcc gtgcttgcgg caccacctcc gccattcttt ctgcgacggg
     2761 cgggtcaaac atgtagtagg gagttgcctc tccggttatg gacctctgcc cgtccttcga
     2821 gctcagccgc gggaagcacc gccgatacca ctcggcgccg ttgtcgaaat gttctggctg
     2881 gtcgaagaag tgcaattcct tacgagcggc gggctctacg agcgggtgac gcgtgagcag
     2941 gtagtagagg aaggatgtcc cacccttttg cgctccaatg atgacgaagt cgggtagcgc
     3001 gcctacgtcc tgctcagccg agggaacgtt cctgacgttc tccaccaaca ccttcatcgc
     3061 gtcgagttcg ttctttaggt gaaagatctc caaactcttc tgtttgagtt ctttcctgag
     3121 cttgatctcg cgttgccgtc tctcccgcag gggctcgagc gccgcggctt gctcctcgag
     3181 caccgcggct tgctcctcga tctgccggtc tttcctgtgc agcgccttcc tctgcctcgt
     3241 tacgaccttc ctgagcctct gggcctgctc ctcgtggtgc ttgggattcg gtaccgctcc
     3301 ccgcgtgagg ccaccctgca tgttcaagaa cgcctttgcg tctcgcccga cgccgaaagc
     3361 cctgttgatt ttgaacactg gcagacctcg acctttcttt acaacgcagc cattctacga
     3421 ttcggacata aagctactgg cctccgggca aattgtcaaa ggatagactc ctctattccc
     3481 gcgccggaga gggtagtgcg ccccagcagg ttggcaaacc cgcaggtgtc cggagagatc
     3541 tagtcagttc cacgatccta cggcggtcct catccgccta tggcccgctc gtatacacgg
     3601 tactcggggg cgaagaactc gcgcaactcg atctcgcact cttctgagag ggagaacgtg
     3661 cgctcgggcg aagtgttcgc agagcccacc t
//
LOCUS       DASTKM010000058         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1244748, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000058 DASTKM010000000
VERSION     DASTKM010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244748"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFQ10_02260"
     CDS             complement(<1..572)
                     /locus_tag="VFQ10_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9964195.1"
                     /translation="MNTEEGHAGGHVQLVDLAKRFGDFEAVCGIDLDIPPGEFFSLLG
                     PSGCGKTTTLRMIAGFERPSGGHILLDGKDVSGTPPNRRNVNTVFQSYALFSHLTVAE
                     NVAFGLRFAKASREEVRSRVGEALSLVRMQEFRDRKPHQLSGGQQQRVALARALILNP
                     SVLLLDEPLGALDAKLRKALQIELKALQEQV"
     gene            complement(569..1948)
                     /locus_tag="VFQ10_02265"
     CDS             complement(569..1948)
                     /locus_tag="VFQ10_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9964196.1"
                     /translation="METVRSWTDGDLRRLDEIIVEGERRFKERQPRSRALIGEARRVL
                     AGGATSSWQIARPQMIWVSHGEGSRIYDADGGEYVDLHGGYGVGAVGHGHPRIVEALT
                     RRVPRGTHFAQPTEDAIAVADELARRFGLPLWRFSNSGTEATMDAVHLMRAIKGRDLI
                     VKVEGCYHGHHDSVMVSVSNEKDEIGPAERPNSAPSGTGIPRAITDLTLIVPYNDLDA
                     LQRLFEEHRGRIAGMILEPIMMNAGVIPPEEGYLEGVREIVHDHGALLAFDEVKCGVT
                     TAYGGASGYLGVSPDMVCLAKAIGGGVPCGAIGGTEEVMGRIADGTYDQVGTFNGNPL
                     TMAASRATLIEILTPEAYEHFDRLGMIMSDGVRDIVEEYDLAAYPLEFGAKGSVTFSG
                     RRVRNYRDFLELDDRFSQAHWLFQHNGGVFLPPWGKAEQWTLSVQHSEDDARRFVDNF
                     ETFARALRR"
     gene            complement(2159..>3238)
                     /locus_tag="VFQ10_02270"
     CDS             complement(2159..>3238)
                     /locus_tag="VFQ10_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9964197.1"
                     /translation="LLVGGYFAYRTTGVILAFLLTILLSIILSAPVNYLARRGLPRTW
                     GVLAVIAALAGLLWLFGLALVPAVETQSRQFAEAFPTLLEEALALANRLQSFFGLGTQ
                     IGLDPESLSGVGREVLTGSTVSTAAGVGLTAATVVSLAAVVFISTIYLVIRPEPWVNG
                     FVSLFPAGWRQRTREVLQTLYHTVQRWFLGQLAAMTFIAVFWAISLSLIGVPFALLLG
                     IFSGLISFIPYLGALISVVLPVLLALISDPFSAVWVILAFIIIQQIEGNLLQPIVMSR
                     AVDLHPALVIFAILVMGTLFGLIGVFLAVPLVAVLQVLVRELWVQKMDQMGTDPNPPA
                     REPPKRKLPGPLSRALKALRPSRHA"
BASE COUNT          626 a         1024 c         1022 g          566 t
ORIGIN      
        1 acttgctcct gcagggcctt cagctcgatc tggagtgctt tcctgagctt ggcgtcgagg
       61 gcgccgagcg gctcgtccag aagcagaacg gatgggttga ggatcaaagc ccgcgcgagc
      121 gcgacccgct gctgctgacc gcctgagagc tggtgcggtt tgcggtcgcg gaactcctgc
      181 atccgcacca gggagagcgc ctctccgacc ctgctacgga cctcctccct ggacgccttc
      241 gcgaaacgca acccgaaggc gacgttctcg gcgaccgtca ggtgagagaa aagggcgtag
      301 ctctggaaca cggtgttgac gttgcgccgg ttcggggggg tgcccgacac gtccttgccg
      361 tcaagcaaga tatgtcctcc gctgggacgc tcgaaaccgg cgatcatgcg cagtgtcgtt
      421 gtcttgccgc acccgctcgg gcctagcagc gagaagaact ctccaggcgg gatatccaga
      481 tctatgccgc acacggcctc gaagtcgccg aagcgcttgg cgaggtctac gagctgtacg
      541 tgaccccctg cgtggccttc ctccgtgttc acctgcgtaa cgcccgcgcg aaggtctcga
      601 agttgtctac gaagcgcctg gcgtcgtcct cggagtgctg taccgagagc gtccactgtt
      661 ccgccttccc ccacggcggc aagaagacac caccgttgtg ctggaagagc cagtgcgcct
      721 ggctgaaacg atcgtcgagc tccagaaagt ccctgtagtt tcgaaccctc ctgccagaga
      781 acgttacgga ccccttggcc ccgaactcca gcgggtaggc ggcgaggtcg tactcctcga
      841 cgatgtcccg caccccgtcg gacatgatca ttccgagccg gtcgaagtgt tcgtaagcct
      901 cgggggtgag gatctcgatc agcgtagcgc gggaggcggc catcgtgagc gggttaccgt
      961 tgaaggtgcc gacctgatcg taggtgccgt cggcgatgcg gcccatgacc tcctcagtac
     1021 cgcctatggc cccgcaggga acgccaccgc ctatggcctt ggcgaggcac accatatcgg
     1081 ggcttacgcc gaggtacccg ctggcccccc cgtaggccgt ggtgacgccg cacttgacct
     1141 cgtcgaaggc gagcagggcg ccatggtcgt gcactatctc gcgcacgccc tccaggtagc
     1201 cctcctcagg aggtatgacg ccagcgttca tcattatcgg ctcgaggatc atgcccgcta
     1261 tccgcccgcg atgctcctcg aagagcctct gcagtgcgtc gaggtcgttg taagggacga
     1321 ttagggtaag gtcggtgatg gcccgcggga tacccgtccc cgaaggcgcg ctattggggc
     1381 gttcggcggg cccgatctcg tccttctcgt tcgacacgga gaccatgacc gagtcatgat
     1441 gcccatggta gcagccctct accttgacga tcagatcccg gcccttgatc gcgcgcatga
     1501 ggtgcaccgc gtccatcgtg gcctcggtgc ctgagttgga gaagcgccac agcggaagac
     1561 cgaaacgccg cgcgagctcg tcagccacgg cgatcgcatc ctccgtcggc tgggcgaagt
     1621 gggtgcccct cggaaccctc cgcgtcaagg cctcgacgat cctcggatgg ccgtgtccaa
     1681 cggcgcctac gccgtacccg ccgtggagat ccacgtactc cccaccgtcg gcgtcgtaga
     1741 tccttgagcc ctctccgtgg ctgacccaga tcatctgcgg acgcgcgatc tgccagctcg
     1801 aggtcgcccc tccagccaga accctccgcg cctccccgat caaagccctc gacctgggct
     1861 gacgttcctt gaatcggcgt tcaccctcaa cgatgatctc gtcgagcctg cggaggtccc
     1921 catcggtcca actccgcaca gtctccatac acgcctcctt taaatgacga ttcaataata
     1981 ggggcatgat gtgtttatgt caacggtatg ggcatgtcag cacgctcagg ccttaaggcc
     2041 ttcgcttgtc agccagtcag taagagcttg cgtaaacgag tagctgacta gctgaaagcg
     2101 cccgacaggg cgcgtgctga ggtgctgaaa tgctcgtcgt gcatgaggct tgagagccct
     2161 atgcgtggcg tgaggggcgt agagctttca aggccctgct gagtgggcct gggagtttgc
     2221 gtttaggagg ttctctggcg ggtgggttgg ggtccgttcc catctggtcc atcttttgca
     2281 cccagagctc gcgcaccaga acctgcagca cggcgacgag tgggacggcc aggaagactc
     2341 ctataaggcc gaagagcgtg cccattacca gtatggcgaa aattaccagt gcgggatgca
     2401 ggtccacggc ccggctcatt acgatcggct ggaggaggtt gccttcgatc tgctggatga
     2461 tgataaaagc caggatcacc cagacggccg agaaagggtc cgagatcaga gccagaagca
     2521 cgggaaggac gaccgagatg agcgcgccca ggtaagggat aaacgagatc aggccgctga
     2581 agatgccgag gagtagcgcg aaaggcaccc cgatcagaga gagcgagatg gcccagaaaa
     2641 cagcgatgaa ggtcatggcc gcgagctgac caaggaacca ccgttgcacc gtgtggtaca
     2701 acgtctgaag cacctctcgc gtcctctgcc gccatccggc ggggaagagc gagacgaacc
     2761 cgttgaccca gggctcgggc cggatgacca ggtagatggt ggagatgaac acgaccgctg
     2821 ccagcgagac cacggtcgcc gcggtcaacc ccacccccgc agccgtcgat acggtgctcc
     2881 ccgtgagtac ctcccgcccg accccggaca ggctttcggg atcgaggcct atctgggtac
     2941 cgagcccgaa gaagctctgg agacggttgg ccagggcgag ggcttcttcc aggagcgtcg
     3001 ggaaagcctc ggcgaactgg cgggattggg tctcgacggc agggacgagc gccaggccga
     3061 agagccagag cagtcccgcc agcgccgcga tcactgccag aacaccccac gttcgcggta
     3121 accccctcct ggccagatag ttgaccggag cgctgagtat gatagatagc aagatcgtca
     3181 gcaggaacgc cagaatcaca ccggtggtcc ggtaggcgaa atacccgccc acgagcag
//
LOCUS       DASTKM010000059         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_187617, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000059 DASTKM010000000
VERSION     DASTKM010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187617"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFQ10_02275"
     CDS             complement(<1..528)
                     /locus_tag="VFQ10_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9964198.1"
                     /translation="MYEVVVIGGGPSGITAALRARELGATVALIESEHMGGVSTNDGC
                     VPTRVLARAARLVRDAEQFADYGLEGEPPAVNFAGLLNHTEHVVRTVHQKKQLRAQLE
                     AADVHVVDGVGDARFLDSHTLTLGNGSSVQGEKFILCVGGHARRIPLPGSEHALTHSD
                     VWTMKGLPESVAVIGG"
     gene            complement(532..774)
                     /locus_tag="VFQ10_02280"
     CDS             complement(532..774)
                     /locus_tag="VFQ10_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964199.1"
                     /translation="MGIVASTLAIAALFDPLRRRIQELRDRRFYRRKYDAARTLRAFS
                     SKLGEETDLNRQGGELVSVVGETMQPVAGANGKKAR"
     gene            complement(928..2349)
                     /locus_tag="VFQ10_02285"
     CDS             complement(928..2349)
                     /locus_tag="VFQ10_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9964200.1"
                     /translation="MVGTYDAVVVGSGPNGLAAAIELARNGRRVAVLEGRSTIGGGTR
                     SAEITLPGFVHDLGSAIHPLGYASPFFRSLPLREHGLEWIHPSAPLAHPFDDGTAVVL
                     ERSIAATAAGLGPDAAAYGRLMEPISADAGRIAYFFIGRPSLLRHPLALATSGMWSLR
                     DARALAESEFDGERARGLFAGNAAHSFLPLDRPPSAMFGLLLGTLGHAFGWPFPKGGS
                     QALADALASYLASLGGEIYTDVAVRSVEDVPRTRAVLFDVTPRQLLDIAGQHFTEDYN
                     SALKRYRYGPGIFKMDFALDGPVPWTAEGCRRAGTVHLGGTLDEIAAGEGAVARGEHP
                     EKPFILLAQQSLFDQTRAPEGKHTVWAYCHVPNGSTFDMTERIEAQIERFAPGFKDLV
                     LEKRVAGTADLERWNPNLVGGDINGGYMDLRQLFTRPMPRINPYSTPAGGLYICSSST
                     PPGGGVHGMCGYLAARAALRYLQ"
     gene            complement(2385..3158)
                     /locus_tag="VFQ10_02290"
     CDS             complement(2385..3158)
                     /locus_tag="VFQ10_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964201.1"
                     /translation="MAEQTPQDEMREEEKHKPEPRDAAYWARYAETLKVAGVAEGATN
                     INVEGRRAVGPLQGFGKMWQKTYRVSLKDADVTPVEVIKTWKENYKDFWPEGNLFYAP
                     LAGITPGEVALISGSLPGGVKLSTGVMVLYADDESFSLMTPEGHPFSGWITFSSFEEE
                     GTTVAQAQVLMRANDPLYEIGLRMGGHKMENEMWRKTLENLAAHFGVNEPVEMNLVCV
                     DPKLQWSHYRNIWQNAGIRSALYTITAPLRWRRNRARQD"
BASE COUNT          588 a         1087 c         1047 g          637 t
ORIGIN      
        1 accgcctatc acggcgactg attctggcag ccccttcatc gtccagacgt cgctgtgggt
       61 cagggcgtgc tcgctacccg gcaaggggat cctacgcgcg tggccgccca cgcagagtat
      121 gaacttctca ccttgcacgc tacttccgtt gccgagtgtg agggtatgac tatcgagaaa
      181 gcgagcgtcg ccaaccccat cgaccacgtg cacgtccgca gcttcgagct gggcccgcag
      241 ctgcttcttc tggtggaccg tgcgtacgac atgctctgta tggttcaaca gcccagcgaa
      301 gttcaccgcg ggcggttcac cctctagccc atagtcggcg aactgctccg catcgcgcac
      361 cagacgcgcg gccctggcga gcacgcgggt cgggacgcag ccatcgttcg tagagacgcc
      421 gcccatgtgc tcgctctcga taagcgcgac ggtggcgcca agctccctgg cgcgcaatgc
      481 tgctgttatc ccggaaggtc cgccgccgat cacgaccacc tcatacatct tctacctcgc
      541 tttcttaccg ttagcgcctg ccacaggttg catggtctcc cctaccaccg aaaccagctc
      601 tccaccctgc ctgttgaggt ccgtctcctc gcccagttta gaggagaacg ctcttagcgt
      661 cctcgcagca tcgtacttcc tcctgtagaa gcggcgatct ctcaactcct gtatgcgccg
      721 tcgaagggga tcgaacaggg ccgcgatagc cagtgtggag gcgacaatgc ccaccgtcgg
      781 ctcctggccg gtcagcgcgc ggagtgtcgt ctgcgcaagg acgccgaagt agaccaggac
      841 gaaagagagc gtcgagaagc aagaaaacca cgcgatcctc ccgacggctg tgcccagggt
      901 ctgcgtcggg ctctccttcc cgccccgtta ctggaggtag cgtaaggccg cacgggccgc
      961 caggtagccg cacatgccgt gcacgccccc acctggtggc gtcgatgagg agcagatgta
     1021 gaggccgccg gcaggagtag agtaagggtt gatcctgggc attggtctgg taaagagctg
     1081 cctgaggtcc atgtacccgc cgttgatatc gccgccgacc aggttggggt tccacctttc
     1141 gaggtcggcc gtccccgcca cgcgcttctc caagacaaga tccttgaaac ccggggcgaa
     1201 gcgctctatc tgcgcctcga tcctctcggt catatcgaag gtcgagccgt ttgggacgtg
     1261 gcagtaggcc cagacggtgt gcttgccctc gggagcgcgc gtctgatcga agaggctctg
     1321 ctgggcgagc aggatgaacg gcttctcagg gtgctccccg cgcgctacgg cgccctcccc
     1381 cgccgcgatc tcgtcgagcg tgccgccgag gtggacggtg ccggcccgcc tgcaaccctc
     1441 cgccgtccag gggaccggtc cgtcgagcgc gaagtccatc ttgaagatgc caggaccgta
     1501 gcggtagcgc ttgagggcac tgttgtaatc ctcggtaaag tgctgcccgg caatgtcgag
     1561 gagctggcgc ggagtcacgt cgaagagtac cgcacgggtt cgcggcacat cttcgaccga
     1621 acgtactgcc acgtccgtgt agatctcacc tcccaaagac gcaaggtagg acgcgagagc
     1681 gtctgcgagc gcctgcgaac cccccttggg gaaaggccag ccgaaggcgt ggcccaacgt
     1741 cccgagtagc agcccgaaca tggcgctcgg tggtcgatcc agcggtaaga aggagtgcgc
     1801 cgcgttgccg gcgaagaggc ccctggccct ctcgccgtcg aactcgctct cggccagggc
     1861 gcgggcgtcc cgcagggacc acatgccgga cgtagcaagc gccaggggat gccggagaag
     1921 gctcggtctg ccgatgaaga agtaggctat cctgccagcg tcagcggaga tcggttccat
     1981 cagccggccg taggccgcgg cgtcagggcc gagcccggcg gccgtcgccg cgatcgaacg
     2041 ctctagcacc accgccgtac cgtcgtcgaa cgggtgggcg agcggggcag acgggtggat
     2101 ccattccagg ccgtgctctc ttagcggcag cgagcggaag aacggcgagg cgtagcccag
     2161 agggtgtatg gctgagccga ggtcgtggac gaagccaggc agcgtgatct ccgccgacct
     2221 cgtgccccct ccgatggtgc ttctgccctc gagcacggcc acacgccgcc cgttgcgggc
     2281 aagctctatc gccgctgcaa gcccgtttgg gcccgagccg acgacgacgg cgtcgtatgt
     2341 ccctaccacg ccccaattgt aacgttcgac tctagagttc gcgactaatc ctgtctcgcg
     2401 cggttgcggc gccagcggag aggagccgtg atcgtataga gggcggaacg tatgccagca
     2461 ttttgccaaa tgttacgata atggctccac tggagcttgg ggtcgacgca gaccaggttc
     2521 atctcgaccg gctcattgac gccgaaatgg gcggccagat tctccagggt cttacgccac
     2581 atctcgttct ccattttgtg gccgcccata cggaggccta tctcgtagag aggatcgttg
     2641 gcgcgcatca gaacctgggc ctgggctacg gttgtaccct cttcctcgaa ggaactgaat
     2701 gtgatccaac ccgagaacgg gtgtccctcg ggcgtcatca aagagaagga ctcgtcatcg
     2761 gcgtacagga ccatcacgcc ggtcgatagc ttgacgccgc ctggcagcga gccggaaatg
     2821 agggcgacct cgcctggcgt aatgccggcc agcggcgcgt agaagagatt cccctcgggc
     2881 cagaagtcct tgtaattctc tttccaggtt ttgatgacct cgacgggcgt tacgtcggcg
     2941 tctttgaggc tgaccctgta ggtcttctgc cacatcttcc cgaagccctg tagaggaccg
     3001 actgccctgc gcccctcgac gttgatgttc gtcgcgcctt cggccacgcc ggcgaccttg
     3061 agcgtctcgg cgtacctggc ccagtaggcg gcatcgcggg gctctggctt gtgcttttcc
     3121 tcctctctca tctcatcttg cggggtctgc tcggccatca tgcgcctccc ttccgtgggc
     3181 gttcgtcgac acgtctctat gccgagttcg cggccctcac ggcctcctct tcagtatcgt
     3241 ggatggcgat ggcgtcatcc aggcgcgtga tctggaagat gctcttgtaa tggtctgata
     3301 gaccgtaggt gagcaacctt tgcttctcgc ggttgatcct aatgagcaac gtaaccaaa
//
LOCUS       DASTKM010000060         5654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_716181, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000060 DASTKM010000000
VERSION     DASTKM010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716181"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VFQ10_02295"
     CDS             complement(<1..1164)
                     /locus_tag="VFQ10_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9964202.1"
                     /translation="MQAVMETYKRLGIAPTGGQGSWLFDSEGNRYLDFIGGIATNSLG
                     HGHPALVGAIQEQAGKLIHCSNLYEIPIQAEVAQMLSVATDFDRVFFCNSGAESIEAA
                     IKLSRKYAHDTYGSNKHEILTFSKSFHGRTYGGLTATAQPTLHDGFGPMLPGMVHATF
                     GDLEAAKALVGPQTAAVLVEPIQGEGGINEPPEGFLEGLRALCDEHGALLIFDEVQTG
                     AGRTGHLYAYQGTDVVPDVLTSAKGLGGGFPVGAMMVKEAYAALGPGNHGSTFGGNPL
                     AMAAVKAVLRVVNDPAFLEEVRFKGTVLKNALQKLAEQVPGAEVRGEGLLLGLDLGDA
                     GLAGRVFEHCLDQGVLVNLVGGTTIRMAPPLTVSRTEVRAALDTFRGGVYQAVE"
     gene            complement(1152..1919)
                     /gene="argB"
                     /locus_tag="VFQ10_02300"
     CDS             complement(1152..1919)
                     /gene="argB"
                     /locus_tag="VFQ10_02300"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET9964203.1"
                     /translation="MKPVVIKVGGGTLAGGALEDLPDLLSAGYRIVIVHGGGLQLTRM
                     LEALHIPTRFHAGLRVTDEETIEVAENVFAGGVNKGLVRELGDLGIQAAGISGTDGPT
                     LVVEPVPDLGRVGKVVRVVPHLIETLWSGGFVPVVAPLGLGPEGAYNVNADSAAAALA
                     VGLAAGYLFVLTDVDGLLEEGETVETLAPDECEAYVESGLAAGGMVPKLRAAAEAAHG
                     GVEARILNGNRKGALEAAVAGERVGTLISEGVAACKQ"
     gene            complement(1916..3073)
                     /gene="argJ"
                     /locus_tag="VFQ10_02305"
     CDS             complement(1916..3073)
                     /gene="argJ"
                     /locus_tag="VFQ10_02305"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET9964204.1"
                     /translation="MAVGVACGVRDAGRRDLGLLFSELPCETAAVFTRNAVWGAPLVV
                     TREAVENGGVRAVVANSGNANAATGERGIEDARAIQALAAETLGIKAGEVAVASTGVI
                     GVHLPMDRISSCISAASGELGEGGEGFAESILTTDTRAKEAVARVEIGGRTVTVGGTA
                     KGSGMIHPNMGTMLAFLTTDAAVEKSCLQETLSRVTDRTFNRVTVDGDTSPSDMALLM
                     ANGAAANEPLTLDSPDYPAFVEAVESVARTLATEIARDGEGATRLVEVAVEGAASEES
                     AAALAKSVVGSNLVKTAVFGQDANWGRVLTAMGYSGEAFDPDGVELWFGSIKVFSGGE
                     PVPHDEAEANATLGAGEVKITVRLSEGNDSATAWGCDLSYEYVRINGSYRT"
     gene            3232..4242
                     /gene="argC"
                     /locus_tag="VFQ10_02310"
     CDS             3232..4242
                     /gene="argC"
                     /locus_tag="VFQ10_02310"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET9964205.1"
                     /translation="MGLKVGVYGGSGYAGIELVRLLAGHPEVGAMSVASRGYVGLRMG
                     DVYPQLAVDGVYVAPEEIEASDLDVAFVAYGHGESAGAVKELLRSGTRLVIDLSADFR
                     LPVEELYVEWYGEHPAPELLAEAHYGLPEVFGAAEGRLVANPGCYPTAAILALAPVVR
                     RIGTGIRSVTINALSGVSGAGAKPSVKTHFVSVNESAGAYGAPRHRHTPEIEMILRRV
                     GEVPEVTFVPHLIPITRGELETIVVDVGDVPEVEEVLGWYAEDYEGWAFVEAREEYPH
                     VAHVANTNRCRLSAAVDSRAGKLLLFSAVDNLLKGASGAALQNMNLALGYEEYLGLEH
                     LE"
     gene            4253..4732
                     /locus_tag="VFQ10_02315"
     CDS             4253..4732
                     /locus_tag="VFQ10_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964206.1"
                     /translation="MGATVSPVDKTTRQDLILRLISERDLGTQQDVVGALADEGVEVA
                     QATVSRDLAELGVLKVGNRYLALPHDPGSAGIEVLPSFVLGLVPAQNQVVIHTRDGTA
                     GAVSSVLDRVKGLKVLGTIAGQDTVLAIAPDASAAAEVASLIADVCRAKTRPAGNVE"
     gene            4845..>5654
                     /locus_tag="VFQ10_02320"
     CDS             4845..>5654
                     /locus_tag="VFQ10_02320"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET9964207.1"
                     /translation="MLKGKKVVLAYSGGLDTSVCVKWLEQQGAEPYALYLDLGQGEPP
                     EDVKAKALQIGATDAFVRDAKAEFAGEYVAPAIKANALYGGKYPLFTALGRPLIAKKL
                     VETAREVGATHIAHGSTGKGNDQVRFDVTTASIAPDLRVVAPVRDWNMSRPEEMAYAE
                     EHAIPVPTTKDSPYSVDENLWGRSIEAGPLEDPDHEPTEDVFALATSPENAPDEPQYV
                     EIGFQEGLPTALDGEELPLVDLISELGAVAGANAVGRIDMVEDRLVGIKSRE"
BASE COUNT         1010 a         1882 c         1793 g          969 t
ORIGIN      
        1 ctctacagcc tggtaaacgc cgcctcgaaa ggtatcgagc gcagcccgaa cctccgttct
       61 gcttacggtt agcggcggcg ccatgcggat ggtcgtgcca cctaccaggt tgacgaggac
      121 cccctgatcc aggcagtgct cgaagactct cccggcaagg ccggcgtccc ccaggtcgag
      181 cccgagcagg aggccttcgc cccgcacctc cgcgccggga acctgttcgg ccagcttttg
      241 gagagcattc ttcaaaacgg tccccttgaa gcgtacttct tcgaggaaag cagggtcatt
      301 caccacgcgg agcacggcct ttactgcggc catcgccagg gggttgcctc cgaaggtcga
      361 gccgtggttg ccaggcccca gcgcggcgta cgcctccttg accatcatcg cccctacggg
      421 gaagccgcca cccaaaccct tggcgctggt gagaacgtcg gggacgacat cggtcccctg
      481 gtaggcatac agatgtcccg tgcgtcccgc gccggtctgg acctcgtcga agatgagtag
      541 cgccccgtgc tcgtcgcaaa gcgctcgcag cccttcgaga aagccctccg gcggctcgtt
      601 tataccgccc tcgccctgga tcggctcgac cagcacggca gctgtctgcg ggccaaccag
      661 cgcttttgcg gcttcgagat ctccgaaggt ggcgtggacc atgccaggca gcatcgggcc
      721 gaagccgtca tgtagcgtgg gctgggccgt ggctgtgagg ccgccgtagg tgcggccgtg
      781 gaaggatttc gagaaagtca ggatttcatg cttgttggaa ccgtaggtat cgtgcgcgta
      841 cttgcgagag agtttgatcg ccgcctctat actctcggct cctgagttgc agaagaagac
      901 cctgtcgaag tccgtcgcaa cggagagcat ctgcgccacc tcggcctgaa tgggaatctc
      961 atagaggttg gagcagtgga tgagcttccc ggcctgctcc tgtatagccc cgacgagcgc
     1021 cgggtggccg tgccccaggg agttcgtggc gatcccgcct ataaagtcca ggtaacggtt
     1081 accctcgcta tcgaagagcc agcttccctg gccccccgtt ggcgctatcc ccagacgttt
     1141 gtaggtctcc atcactgctt gcatgccgcg actccttcgg atatgagagt tccaacccgc
     1201 tcgccagcga cagccgcctc gagcgcacct ttcctgttgc cgttgaggat cctggcctcc
     1261 accccgccgt gtgcggcctc ggccgcagcc cgcagcttgg gcaccatccc gcccgccgcg
     1321 agcccgctct cgacgtaggc ctcgcactca tccggcgcga gcgtctccac cgtctcgccc
     1381 tcttccagca gcccatcgac gtcggtgagt acgaacaggt agccggccgc gagtccgacc
     1441 gcgagcgccg ccgcggccga gtcggcgttg acgttgtagg cgccctctgg accgagcccg
     1501 agcggcgcga cgacggggac gaaccctcca ctccagaggg tctcgatgag gtgaggcacg
     1561 actcgcacga ccttgccgac cctgcccaga tccgggaccg gctcgaccac tagcgttggc
     1621 ccgtcggtcc ccgagatgcc cgcggcctgt atcccgagat ctcccagctc gcgcacgagg
     1681 cccttgttca cgcctccggc gaacacgttc tcggccacct cgatagtctc ctcgtccgta
     1741 accctgagcc ccgcatgaaa ccgcgtcggg atgtggaggg cctccagcat cctggtgagc
     1801 tggaggccgc ctccatgtac gatgacgatc ctgtagccgg ccgaaaggag atcaggcagg
     1861 tcctctagcg cccctccggc aagcgtgccg ccgccaactt tgatcaccac gggcttcacg
     1921 tcctgtagct cccgttgatc ctgacgtact cataactcag gtcgcaaccc caggctgtgg
     1981 ctgaatcgtt accctcgctc aaccggactg tgatcttcac ttcgcctgcc ccgagggtcg
     2041 cgttggcctc agcctcatca tgtggcacag gctctccgcc cgagaagacc ttgatagaac
     2101 cgaaccagag ctcgactccg tcaggatcaa aagcctcccc cgagtaaccc atcgccgtga
     2161 gcacccttcc ccagttcgcg tcctgaccga acacggccgt cttgaccagg ttgctcccca
     2221 ccacgctctt cgcaagcgcc gccgcggatt cctcgctcgc cgcgccctcc acggctacct
     2281 cgacgagcct ggtcgccccc tcgccgtcgc gtgcgatctc cgtggccaat gttcgcgcta
     2341 cgctctcgac agcctccacg aaggcaggat agtcagggga gtcgagcgtg agcggctcgt
     2401 tagccgcggc gccgttcgcc attagaaggg ccatatcgct cggggaggtg tctccgtcca
     2461 cggtaacgcg gttgaaggtg cggtccgtga cccggctcag agtctcctgc agacagctct
     2521 tctcgacggc ggcgtctgtc gtcagaaacg cgagcattgt gcccatgttc gggtggatca
     2581 taccgctccc cttggccgtc cccccgaccg tgacggtcct gcccccgatc tccacccgtg
     2641 caaccgcctc ctttgcacga gtatccgtgg tcaagatgga ctcggcgaag ccctcaccgc
     2701 cctcacccag ctcgcccgac gccgcactta tacaggatga gatcctgtcc atcggcaagt
     2761 ggacgccgat gacaccggtc gaagctaccg cgacctctcc ggctttgatc ccgagcgtct
     2821 cagccgcgag cgcctgtatc gcgcgggcgt cctcgatgcc ccgctcgccg gtcgccgcgt
     2881 tggcgttgcc gctgttcgcc acgaccgccc gcaccccacc gttctcaaca gcctcccgcg
     2941 tcacaacgag cggcgcgccc cagacggcgt tgcgggtgaa cacggccgcc gtctcacagg
     3001 gaagctcgga gaagaggagc ccaagatccc gcctcccagc gtcgcgtacc ccacaagcaa
     3061 ccccaaccgc cataaatccc gaggtagccg tcacaccaat ggagagagac tttattctga
     3121 ttgttgaatc tttattcacc acgggggcgt attgtcgcac ccacgtgcga aggcgtcaac
     3181 cagtatatgc agaaacttga ataaatatcc gaaagttcta caatgggcgg catgggacta
     3241 aaggtcggcg tctatggggg gagtgggtac gcggggatcg agctcgtacg gctgctcgcc
     3301 ggccaccccg aggttggggc gatgagcgtt gcgtcgcgcg ggtatgtggg gttaaggatg
     3361 ggggacgtct acccgcagct tgccgtcgat ggggtctatg tggcgccgga ggagatcgag
     3421 gcctccgacc tcgacgtggc cttcgtagcc tacggacacg gcgagagcgc cggcgcggta
     3481 aaggagttgc tgagatcggg gacccgcctg gtaatagacc tctctgcgga tttccgcttg
     3541 cccgtggagg agctatacgt ggagtggtac ggggagcatc ctgcgcccga gcttctcgcg
     3601 gaggcgcact acgggttgcc cgaggtcttc ggggctgctg aggggcggct cgtcgccaac
     3661 ccgggttgct atccgacggc ggccattctg gctcttgctc ccgtcgtaag gcgcataggg
     3721 acgggtatac gctccgttac catcaacgct ctatccgggg tgagcggggc aggggcgaag
     3781 ccgagcgtga agacgcactt cgtcagcgtg aacgagagtg ctggcgccta cggggcgccg
     3841 cggcacaggc acacgcctga gatcgagatg attctgcggc gtgttgggga ggtgcccgag
     3901 gtgacgttcg taccgcacct catccccata acgcgtgggg agctcgagac catcgtggtc
     3961 gacgtcgggg acgtgcctga ggtggaggag gtcctcggct ggtatgcgga ggactacgag
     4021 ggatgggctt tcgtcgaggc gcgggaggag tacccgcacg tcgcgcacgt ggccaacacc
     4081 aacaggtgca ggctctcggc tgcggtagat agtcgggcag ggaagttgtt gctcttctcg
     4141 gcggtcgaca acctgctcaa gggcgcctcg ggggcggcgc tacagaacat gaacctggcg
     4201 ctcggctacg aggagtacct ggggctcgag cacctcgagt aggaggacag ttttgggagc
     4261 gacggtgtca ccggtggaca aaacgacgag gcaggatctg atactgcgtt tgatctcgga
     4321 gcgcgacctc ggcacccagc aggatgtcgt cggcgcgctg gcagacgagg gggtggaggt
     4381 cgcgcaggcg accgtgagcc gcgacctcgc cgagctaggg gtcctcaagg tcgggaaccg
     4441 ctacctggcg ctgccgcacg accccggttc cgcaggcatc gaagtcctac ccagtttcgt
     4501 cctcggcctc gtgccagctc agaaccaggt cgtcatccac acccgcgacg ggactgctgg
     4561 cgccgtctcc agcgtcctcg acagggtgaa ggggctcaaa gtgctcggca ccatcgcggg
     4621 ccaggacacc gtgctcgcca tagccccaga cgcgtccgcg gccgcagagg tggcgagcct
     4681 catcgccgac gtctgcaggg cgaagacgcg gccagccggc aacgtcgagt agtcgaggac
     4741 cccaggagtt tacggacgcc actgcgcctg gatccgacct caccctgacg gtgctaggag
     4801 aaccaactac cgtctgaggc catagatacg tttcgggagg gatcatgttg aaaggaaaga
     4861 aggtcgtact cgcctactcg ggcggcctgg acacttcggt ctgcgtcaag tggctcgagc
     4921 agcagggggc ggagccctac gcgctgtatc tggacctcgg ccagggggag cctccggagg
     4981 acgtaaaggc gaaggcgctc cagataggcg ccactgacgc cttcgtgcgg gacgcgaagg
     5041 cggagttcgc cggagagtac gtcgcccctg cgataaaagc gaacgcgcta tacggcggga
     5101 agtacccact cttcacggcc ctcgggcgcc cactcatagc gaagaagctc gtcgagactg
     5161 caagggaggt tggggcgacg cacatcgcgc acggctcgac ggggaagggc aacgatcagg
     5221 tacgcttcga cgtgacgaca gcctccatcg ctccagacct gagggtcgtc gccccggtgc
     5281 gcgactggaa catgagccgc cccgaggaga tggcctacgc cgaggagcac gccatccccg
     5341 tcccaacgac gaaagactcc ccctacagcg tggacgagaa cctctggggc cgctctatag
     5401 aggcagggcc actggaagac cccgaccacg aacccaccga ggacgtcttc gcgctcgcga
     5461 cctctcccga gaacgccccc gacgagccgc agtacgtgga gatcggcttc caggaaggac
     5521 tgccaacggc gctcgatggg gaagagctgc cgctagtaga cctcatatcc gaacttggcg
     5581 ccgtcgccgg cgcaaacgcc gtcggccgca tcgacatggt cgaggaccgc ctggtcggaa
     5641 taaagagccg cgag
//
LOCUS       DASTKM010000061         4856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_363661, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000061 DASTKM010000000
VERSION     DASTKM010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363661"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFQ10_02325"
     CDS             <1..282
                     /locus_tag="VFQ10_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HET9964208.1"
                     /translation="ILGTDDAMAVSLPRLEIQADDVKCSHGSTTGQVDDVELFYLMSR
                     GIPRREAEKLVVFGFFGEVTSRIPLKGLKDKLDRAIEGKIGLGFEERVA"
     gene            287..634
                     /locus_tag="VFQ10_02330"
     CDS             287..634
                     /locus_tag="VFQ10_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET9964209.1"
                     /translation="MAEFHKIAKTGDVAPGEVGVYELEGHRVALCNVDGEFHAIEDVC
                     THDAGPLDQGELIGNQIKCPRHGAKFDVTTGKALTLPAIKPVPKHDVKVEGDDIYVAL
                     AEAKVTGTRRRRR"
     gene            641..1117
                     /locus_tag="VFQ10_02335"
     CDS             641..1117
                     /locus_tag="VFQ10_02335"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET9964210.1"
                     /translation="MLLEGSCHCEAIRFRVESETPYPYQACYCSICRKTAGGGGYAIN
                     LGADANTLEVEGKENLSVYNAKLYDRGEVPASEAERNFCRFCGSFLWLYDPRWPDLIH
                     PFASAIDTPLPKPPEHVRLMLNYAPTWCEIHTGEGESLFPEYPEESLEEWHQRHEL"
     gene            1179..1586
                     /locus_tag="VFQ10_02340"
     CDS             1179..1586
                     /locus_tag="VFQ10_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET9964211.1"
                     /translation="MQSFYREIVLDHFQRPRNRRELEDADIYEHIDNPLCGDVVTVYA
                     KVHDGSFEEVSFTGRGCSISQASASMLTEQLTGKSLEEAEEKIEAFLEMMRTDEREEL
                     GELSALKGVVQTPNRIRCATLAWDAMRRALPTL"
     gene            1691..3178
                     /locus_tag="VFQ10_02345"
     CDS             1691..3178
                     /locus_tag="VFQ10_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET9964212.1"
                     /translation="METEEQGSAGRPAPDAFRSERLDRALARASDAPLREGNRLELLR
                     NGPDTYEDWLAAISGAERWVHLDNYIFSDDEVGERFARALILKAEEGVSVRVLYDWFG
                     CLDVSRSFWRRMREAGVDVRAVNPPTLGGPLGAIRRDHRKLLAVDGEYASTGGVCISD
                     GWLVTSPETGLPYRDTAVGIRGPAVAEVERAFAGLWSVAGESLPDAECPDSENIPKAG
                     EVPARVVVQEPRRMRTLRMLQLLTAGVERRFWITDAYFLSMAILSQSLMSVVRDGVDV
                     RVLVPATNDLPWIGALSRAGYRQFLEAGVRIFEYGGPMIHAKTLVADGWWSKVGSTNL
                     NVASLVANWEIDLVAEDIGFAAQMEELFEEDLSNAREVLLAGSAAHPRVRPERPIQFS
                     DRDARRGVAGSGSGGSATILRVGSTALQKSGYPLETHEHALAAAASGALLGASLLSLR
                     FPRFLAWPLATVGALYGGLGVLRAARSTLSDRWPKPPAQQDVDDP"
     gene            complement(3206..4510)
                     /locus_tag="VFQ10_02350"
     CDS             complement(3206..4510)
                     /locus_tag="VFQ10_02350"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET9964213.1"
                     /translation="MGPGEWQRRIDRANRERLQQQRELGMVPDDEVHPTDYVPRILPA
                     ADWAVLERGLAQRMLAINEWLRRLEAGKDEVVPEEVVESSTLFDASIPTRFGDVPNRQ
                     MGFDIVAVERSGRTGGWEYLVIEDNAKMPVGIEPMNILRARTPDVLPESYTVLRVKPL
                     EGLMERFGEVVRAASPKPDPTMAILSTGPHDQYYLDHKVLASELGAILAERHEVEVDR
                     DGRLVHKKSGSKIDIIYERIEDGRIYDDVKGLIESQARGLIDAVFAPNLGVADDKGVY
                     PFIPEMIRTYLGEEPVLNNVETYSLAVEDARRYVMDHFDELVIKSRSGWGGKDVLISP
                     EESREDIEEFRRMVEENPVEYVAQEVIDFSTHVLCETDEANDGFVLRDAHADYRIHAL
                     APDAQTIEVVPGAMTRVAAPGSKLVNISSGGKMKDTWVQCGA"
BASE COUNT          905 a         1541 c         1538 g          872 t
ORIGIN      
        1 atcctcggta ccgacgacgc gatggcggtc tccctgccac ggctggagat tcaggccgac
       61 gacgtcaagt gctcccacgg ctcgaccacc ggccaggtgg acgatgtgga gctcttctat
      121 ctcatgagcc gcgggatccc acgccgcgag gccgagaagc tcgtcgtgtt cggtttcttc
      181 ggggaggtca cgagcaggat ccccctcaag ggcctgaaag acaagctcga ccgggctata
      241 gagggcaaga tcgggctcgg cttcgaggag cgggtggcct aaccggatgg ccgagttcca
      301 caagatagcc aaaactggcg acgtggctcc cggcgaagtg ggcgtctacg agctggaggg
      361 ccaccgggtt gctctgtgca acgtggacgg cgagttccac gccatcgagg acgtatgcac
      421 ccacgacgcc ggtccactgg atcagggaga gttgatcggc aaccagataa agtgtccgcg
      481 ccacggcgcc aagttcgacg tgaccaccgg caaggcactc accctcccgg ccataaagcc
      541 cgtccccaaa cacgatgtca aggtcgaggg cgatgacatc tacgtggcgc tggcggaagc
      601 caaggttacc gggaccagac gccgcaggag gtaggcgagc atgctgctcg aaggttcctg
      661 ccactgcgaa gcgatacgtt tccgggtcga gtcggagacg ccgtacccgt atcaggcttg
      721 ctactgctcc atctgccgca agactgccgg gggcggcggc tacgccataa acctcggagc
      781 cgacgcgaac accctggagg tcgaaggtaa agagaacctc tcggtctaca atgcgaagct
      841 ctacgaccgt ggtgaggtac cggccagcga ggcggagcgc aacttctgca ggttctgcgg
      901 gagcttcctc tggctttacg acccgcggtg gcccgacctt atccacccct tcgcctcggc
      961 gatagacaca cccctcccca aacctccaga gcacgtccgc ctgatgctga actacgcgcc
     1021 aacgtggtgc gagatccaca ccggagaagg cgagagcctt tttcctgagt atcccgagga
     1081 gtcactcgag gagtggcacc aacgccacga gctctaacct cggcgatgca gcaacacgcg
     1141 aagctacgca cgctcctgat acaatttcct gcaaggtgat gcagagcttc tacagagaga
     1201 tagttctcga ccactttcag cgcccgcgca accggcgcga gctggaggat gccgacatct
     1261 acgaacacat tgataatcct ctctgcggcg acgtggtgac ggtctacgcg aaggtgcatg
     1321 atggcagctt cgaagaggtg agctttaccg gacgcggatg ctccatctcg caagcgtctg
     1381 cctcgatgct cactgagcaa ctcaccggca agagcctcga ggaggcggag gagaagatcg
     1441 aagccttcct cgagatgatg cgcacggacg aaagagaaga gctcggggag ctctccgccc
     1501 tcaaaggcgt cgttcagacc cccaaccgca tcaggtgcgc caccctcgcc tgggatgcca
     1561 tgaggcgcgc gctccccact ctataaatac cgccggcacc gtacattgcc acgtcgcagg
     1621 accaccttcc gtcgacgtga gcgtagcaac ggggtagagt tagactgtac tctccgcgag
     1681 gaggcggggg ttggagacgg aagagcaagg atctgcggga agaccggcgc cggacgcgtt
     1741 ccggagcgag agactggacc gcgcgctggc gcgtgccagc gacgcgccgc tccgggaggg
     1801 caaccggttg gagcttctca ggaacggccc cgacacttac gaggactggc tcgcggccat
     1861 ctccggggct gagcgctggg tgcatctgga caactacatc ttcagcgacg acgaggtcgg
     1921 ggagaggttc gcgagggctt tgatcctgaa ggccgaggag ggtgtgagcg tccgtgtgct
     1981 ctacgactgg ttcggctgcc tcgacgtgag ccgctccttc tggcggcgga tgcgggaggc
     2041 cggcgttgat gtcagggcgg tcaacccgcc gacgctcggt gggccgctcg gcgcgatccg
     2101 gcgcgaccac cgcaagctgc tcgccgtgga cggcgagtac gcctcgacgg gcggcgtgtg
     2161 catctccgac ggctggctcg tgacctcccc cgagacaggg ctcccataca gggacacggc
     2221 cgtcggcatc cgcggcccgg ccgttgccga ggtggagcgc gccttcgcag gactttggag
     2281 cgtcgccggt gaatctctcc ccgatgccga atgccccgac tccgagaaca tcccaaaggc
     2341 cggcgaggtg ccggcgcgcg tggtcgtcca ggaaccacgc aggatgcgga ccctgcggat
     2401 gctgcagttg ctcactgcag gggtcgagcg caggttctgg atcaccgacg cttacttcct
     2461 ctctatggcg atcctctccc aatctctgat gtccgtagtc cgtgacgggg tggacgtgcg
     2521 ggttctcgtg cctgcgacca acgatctgcc ctggatcggg gcgctctccc gcgcgggcta
     2581 ccggcaattc ctcgaggcgg gggtccgcat cttcgagtac ggtggcccca tgatccacgc
     2641 caagaccctg gtcgccgacg gctggtggtc gaaggtcggc tcgacgaacc tgaacgtcgc
     2701 cagcctcgtc gcaaactggg agatagacct ggtggcagag gatatcggct tcgccgccca
     2761 gatggaagag ctattcgagg aggatctctc caacgcccgc gaagtcctcc tcgccgggtc
     2821 tgccgcgcac cccagggtgc gtccggagcg cccgatccag ttctccgacc gcgatgcccg
     2881 taggggtgtg gcagggagcg gttccggcgg ttcggccacc attctacgtg tcggcagcac
     2941 tgccctccag aagagcggct atccgctgga gacccacgaa cacgccctgg cagccgcggc
     3001 cagcggtgcc ttgctcggag cctcccttct cagcctccgt ttcccgcgct tcctcgcctg
     3061 gcctctggcc acggtcggcg ccctctacgg gggtctcggg gtgctgcgcg ccgcgcgctc
     3121 gaccttatct gaccggtggc cgaaaccacc cgcccagcaa gacgtcgacg acccctgata
     3181 gcgggttggg gaaggctcag aagccttatg caccacactg cacccaggtg tccttcatct
     3241 tgcctccgct ggagatgttg accagcttgc tgcccggcgc cgctaccctc gtcatggcgc
     3301 ctggtactac ctcgatcgtc tgggcgtctg gggcgagagc gtggatgcgg tagtcggcgt
     3361 gggcgtcgcg tagaacaaag ccatcattag cttcgtctgt ctcgcagagg acgtgggtcg
     3421 agaaatctat cacctcctgg gccacgtact cgacagggtt ctcttctacc atccggcgga
     3481 actcctcaat gtcctccctg gactcttcgg gagagatgag gacgtcctta ccgccccagc
     3541 cactgcggct ctttatgacc aactcgtcga agtggtccat gacgtagcgt cgggcatcct
     3601 cgacggcgag cgagtaagtc tccacgttgt tcagtaccgg ctcctctccg aggtaggtcc
     3661 gaatcatctc ggggatgaac ggatagacgc ccttatcgtc ggcgacgcca aggttgggag
     3721 cgaagacggc gtcgatgagg cctctggcct gagattcgat caggcccttt acgtcgtcgt
     3781 agatcctgcc gtcctcgatg cgctcgtaga ttatgtcgat cttactaccg ctctttttgt
     3841 ggacgagcct tccgtcgcgg tcgacttcga cttcgtgccg ctcggccagg atcgccccga
     3901 gctcgctggc gaggaccttg tgatcgaggt agtactggtc gtgaggccct gtggagagga
     3961 tcgccatagt cgggtcgggc ttgggcgaag cagcccgcac gacctcgccg aagcgctcca
     4021 ttagcccttc gagcggtttc acccggagca ccgtgtagga ctcgggtagt acatcggggg
     4081 ttctcgccct gagtatattc atcggctcga taccgaccgg catcttggca ttgtcctcga
     4141 tgacgaggta ttcccacccg ccggtccggc cggaacgctc gacggcgact atgtcgaagc
     4201 ccatctgccg gtttgggacg tccccgaatc gggttgggat agaagcgtcg aagagagtgc
     4261 tgctctcgac gacctcctcg ggtacgacct catcctttcc tgcctcgaga cgtctgagcc
     4321 actcgttgat cgcgagcatc cgctgcgcca gcccccgctc caacactgcc cagtccgcag
     4381 ccggcaagat gcggggcacg tagtcggtcg ggtggacctc atcgtcaggg accatgccca
     4441 actctcgctg ctgctggagc cgttccctat tcgccctgtc aatcctccgc tgccattccc
     4501 cagggcccat ccgccccatc tcctcgagca cgggccggta atgagaatag ggctcgccag
     4561 cagggccgaa gacctcgttg ggagaagaca aaccgcgctc gaagcgtatg gagcggtcag
     4621 gcatcagtta acccagagga gcttcaagct gcaggttaca ggcatcagca agacgatccg
     4681 aagtcggagg tcggctggac gacggatcca acctgatacc tgtaatctac tcatccgaat
     4741 attcccgtga ggacacaggg ggagcccagg cagaaagaga gcgagaaggt gacggcgaag
     4801 aagacgttct cgtcagaggt ataggagtag aggcgcttgt agtggaacat caggag
//
LOCUS       DASTKM010000062         4515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1420999, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000062 DASTKM010000000
VERSION     DASTKM010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420999"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VFQ10_02355"
     CDS             complement(<1..882)
                     /locus_tag="VFQ10_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9964214.1"
                     /translation="MPIRWRLTLFIALAIGAILLVLGVALFIFLRAALLKNVEETARM
                     RAIHAAKTISAEDSLEKEDVSEYTLDGVFVIVRDGKGGVLPETVNIQPKDAAKDHVWQ
                     EALADGDPASGRAEITGDDPYYIYAVPVDPAHGDARVVEAGKSFEAALDGVQAVGVIL
                     AAGIGVAFLLSIGGAYLLARTALRPVEAVTATAQEMGEGDLAKRLPVANPKDEIGRLT
                     TTINGLLARLDAAFRRRQEALSRQRRFAADASHELRTPLTSISGHARMLDEWALEGDK
                     ETARRSVGTIRREAGRMR"
     gene            complement(887..1564)
                     /locus_tag="VFQ10_02360"
     CDS             complement(887..1564)
                     /locus_tag="VFQ10_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9964215.1"
                     /translation="MRPGTRILLVEDDRAIAGFVAPELEHLGFQVRCAYDGVTGLEDA
                     QKFGPALIVLDIMLPGLDGVGVLKRLREGGSRVPVIMLTARDTTLDKVHSLDRGADDY
                     LTKPFDIEELLARIRALLRRAEGEEILRVADLEINTSTREVRREEREIELTTREYELL
                     EYMAQNPRRVLSRELLLSRVWDEELGLTTNVVDVYVGYLRRKVDAPGEEKLIRTVRGA
                     GYALKGA"
     gene            1902..3053
                     /gene="sucC"
                     /locus_tag="VFQ10_02365"
     CDS             1902..3053
                     /gene="sucC"
                     /locus_tag="VFQ10_02365"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET9964216.1"
                     /translation="MDLYEYQGKELLREYGLETLPGIVAGTSEEAGRAAETLGGTVAI
                     KAQVLTGGRGKAGGIKVVDSSREAEEAAGQILGMEIRGHTVRRVYVEHGEEIDREFYL
                     SITVDRTAKRPLILFSTEGGVEIEEVAESNPEAIVRLHVDPLLGLLPYQVREITFAAG
                     LEGDVAKGVGSTLSHLYKAFEGLDASLVEINPLVVTGEGRVLTLDAKVTVDDSSLYRH
                     EHIAGLHDVEAADPQEERAQEVGLQYVKLDGDIGILGNGAGLVMSTLDVVAQAGGKPA
                     NFCDVGGGADAEQISTALDIVTSNEQVRSVLFNIFGGITRGDEVARGLLSAIERTGLT
                     LPIVVRLDGTNAEEGRRILAENTPENVVTEETMLEAARRAVELARDGER"
     gene            3058..3933
                     /gene="sucD"
                     /locus_tag="VFQ10_02370"
     CDS             3058..3933
                     /gene="sucD"
                     /locus_tag="VFQ10_02370"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET9964217.1"
                     /translation="MAILVDDGTRLLVQGITGREGAFHTGRMLQSGTRVVAGVTPGKG
                     GRDHEGVPVFDTVEEAVEETGANASVIFVPPPFAADAIFESLNAGLSTICCITEGIPV
                     HDMMRVANYLGTKDATLVGPNCPGVLSPGKANVSIMPKDIFMPGNVGVVSRSGTLTYQ
                     IVNELTQREIGQSTAVGIGGDPIIGTDFIEILSKFEADAETECAVMIGEIGGNAEQLA
                     AEYVKSEMSIPVVAYIAGFTAPEGKTMGHAGAIVSGGESTAEAKSEALREAGIRAATN
                     PSEVAELVAEALGRA"
     gene            4113..>4515
                     /locus_tag="VFQ10_02375"
     CDS             4113..>4515
                     /locus_tag="VFQ10_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964218.1"
                     /translation="MRWLLEERIGEVQGPEAREGEEQLAWWQVVCLTSVDYFSPWLHT
                     RDCGHRRKGTEEEYARKERKQRKDNHISPGEQILILEVQVEDAQEFMDVLEVRGVEVG
                     DQQMLRSISSVVPNAIAAFLLPLRDTTGKTSH"
BASE COUNT          885 a         1398 c         1449 g          783 t
ORIGIN      
        1 gcgcatcctc cctgcctccc tgcggatggt gccgacgctc cggcgcgccg tctctttgtc
       61 tccttcgagg gcccactcgt cgagcattct ggcgtgcccg ctaatggagg tcaggggggt
      121 tctcagctcg tgactcgcgt cggcggcgaa gcgccgctgg cgcgagaggg cctcctgcct
      181 gcgcctgaag gcggcatcga gacgcgccaa caggccgttt atcgtggtgg tgaggcgtcc
      241 gatctcatct ttcgggttcg caacgggcag ccgcttggcg agatcaccct cccccatctc
      301 ctgcgccgtg gccgttacgg cttcgacagg ccgcagggcc gtacgcgcca gcaggtaggc
      361 gccgccgatg gagaggagga aggccacccc gatgcctgcg gcgaggatta ctccgacggc
      421 ctgtacacca tccaacgccg cctcgaagga cttccctgcc tccacgaccc ttgcgtctcc
      481 gtgcgccggg tccacaggca cggcgtagat gtaatacgga tcgtcaccgg ttatctcggc
      541 cctgcccgag gcagggtcgc cgtcggcgag tgcctcttgc cagacgtgat ccttggctgc
      601 gtccttcggc tggatgttga ccgtctcggg gagcacacct ccctttccgt ccctgacgat
      661 cacgaagacc ccgtccaagg tatattccga gacgtcctcc ttttccaggg agtcctctgc
      721 acttatcgtc ttggcggcat gaatggccct cattctggcg gtctcctcga cgttcttgag
      781 cagggcagcc ctgaggaaga tgaagagtgc cacgccaagg accaggagta tcgctcctat
      841 tgcgagggcg atgaacagcg tcaggcgcca cctgataggc aagtgctcag gcccccttca
      901 aggcgtagcc agccccacgc acggtacgga tcagcttctc ctcaccaggg gcgtccacct
      961 ttctcctgag atagccaacg tagacatcca ccacgttggt ggtaaggcct aactcctcgt
     1021 cccagacgcg cgagagcagg agctcgcggg agagcacccg gcgagggttc tgagccatat
     1081 actcgagcag ctcgtactcg cgggtcgtta gctctatctc cctctcctcc cgcctcacct
     1141 cacgggtgga ggtgttgatc tccaggtctg ccacacgaag tatctcttca ccctcagccc
     1201 tccttagcag cgcgcggatg cgggcaagga gttcctctat gtcgaacggc ttggtcaggt
     1261 aatcgtccgc cccacggtcg aggctgtgta ccttgtcgag cgtggtgtcg cgggctgtca
     1321 gcatgatcac gggcaccctg ctgcccccct cgcgcaatcg cttgaggacc ccgaccccat
     1381 ccaagcccgg cagcatgata tccaggacga tcaacgcagg cccgaatttc tgcgcatcct
     1441 ccaagccagt gacaccgtcg taggcacacc gcacctggaa gccgaggtgt tcgagctcgg
     1501 gtgcgacgaa gccggcgatc gctcggtcgt cttctaccaa cagtatcctg gtacctggtc
     1561 tcatctactc ctctttggcc tctggaccga tattgtactt ccggtcgtcc cgactctgct
     1621 aggatcgaag atcgacgaga tacctccagc gcacgcaaag ctgcggatct gcgcacctca
     1681 acccctccgc tcatttgccc aggctggtcc gtcggatgct aacagcgggt gcacgagggt
     1741 ccactctaga caccctctct gagagggagg tgagaacctg caaggccgag cggtcccgca
     1801 tcggggcgcg tacggcgggg aggttcctgg gatcatgatt agggtttgga agtctgtata
     1861 atttcggtcc gtcatggccg acttcgcggg agtcttctaa cttggatctc tacgagtatc
     1921 aagggaaaga actgttgagg gaatacggcc tggagacgtt gcccggcatc gtcgcgggta
     1981 cgtctgagga ggcaggaagg gccgccgaga cactcggggg aacagtggcg atcaaagccc
     2041 aggtccttac cggagggcgc ggcaaggccg gcggcatcaa ggtggtggac agctcgcggg
     2101 aggccgaaga ggcggcgggg cagatcctcg gtatggagat ccgcgggcac accgtgcggc
     2161 gcgtctacgt cgagcacgga gaggagatag acagggaatt ctacctctcc atcacggtag
     2221 acaggacggc gaaacggccc ttgatcctgt tctccacaga gggcggggtg gagatcgaag
     2281 aggtcgcgga gtcgaacccc gaggcaatcg tcaggctcca cgtagaccca ctcctcggtc
     2341 ttcttcccta ccaggtgcgt gagatcacct tcgccgcggg gctggaagga gacgtcgcca
     2401 agggcgtagg atcgacgctc tcgcacttgt acaaggcctt cgagggtctc gacgcgagcc
     2461 tggttgagat caaccccctc gtcgttaccg gggaggggcg cgtcttgacg ttggacgcga
     2521 aggtaactgt ggacgactcc agtttgtaca ggcatgaaca catagccggg ctgcacgacg
     2581 tcgaagccgc agacccgcag gaggaacggg cacaggaggt cgggcttcag tacgtcaagc
     2641 tcgacggcga tatcggcatc ctcggcaacg gggcggggct ggtgatgagc acgctcgacg
     2701 tcgtcgccca ggccggcggc aaaccagcca acttctgcga cgtcggcggc ggggccgacg
     2761 ccgagcagat ctcgaccgcc ctcgatatcg tcacttccaa cgaacaggtc aggagcgtgc
     2821 tcttcaacat cttcggcggc ataacccgcg gcgacgaggt ggcgcggggg ttgctgtcgg
     2881 ccatagagcg gacagggctc acgctcccca tcgtcgtcag gctcgacggt acgaacgccg
     2941 aagagggacg ccggatcctc gccgagaaca caccggagaa cgtggtcaca gaggagacga
     3001 tgctggaagc ggcccgcagg gcggtagagc tcgctagaga cggggagagg taacttcatg
     3061 gcgatactcg tagacgatgg gacgcgtctc ctcgttcagg gcataacggg cagggagggc
     3121 gcgttccata caggaaggat gctacagagc ggcaccaggg tagtcgcagg ggtgacgccc
     3181 ggtaagggcg ggcgggacca cgaaggtgtc cctgtcttcg acaccgtcga ggaggccgta
     3241 gaggagacgg gcgcgaacgc gagcgtgatc ttcgtccccc cacccttcgc cgccgacgcc
     3301 atcttcgagt ctttgaacgc cggactctcg accatctgct gcattactga aggcatcccc
     3361 gtccacgaca tgatgcgggt cgcaaactac ctggggacca aggacgccac cctcgtcggg
     3421 ccgaactgtc ccggcgtcct ctcgccgggg aaagcaaatg tctccataat gccgaaggac
     3481 atctttatgc ccggaaacgt cggcgtcgta agccgcagcg gcacgctgac ctaccagatc
     3541 gtcaacgagc tcacgcaaag agagatcggg cagagcaccg ccgtcgggat cggcggcgat
     3601 ccgataatag ggactgactt tatcgagatc ctcagcaagt tcgaggccga cgccgagacc
     3661 gaatgcgctg tgatgatcgg ggagatcggg ggcaacgcgg aacaactcgc agccgagtac
     3721 gtcaagagcg agatgagcat ccctgtcgtg gcctatatcg ccggcttcac ggccccagag
     3781 ggcaaaacca tgggacacgc tggcgccatc gtctctgggg gcgagagcac ggcggaggcc
     3841 aagagcgagg cgctcaggga ggcaggcata agggccgcga cgaatccttc ggaggtcgcc
     3901 gagctcgtcg ccgaggcgct gggccgggcc tgaagctcga ggaggcctga aggacgaaag
     3961 agatcctgac ccctcgatct ccgatatcct atacttcctg ggctcaccat gaacgggtat
     4021 cgtccaaccc aggcaatccg gacctccccg atcgaggcac gaacgtaggt gccgaatcca
     4081 ccgtttccaa gcagcatgcc ggcaggcggt tcatgcgctg gctcctcgag gagcgcatcg
     4141 gagaggtgca ggggcccgag gccagggagg gagaagaaca gctcgcgtgg tggcaggtcg
     4201 tgtgcttgac cagcgtggac tacttttccc cttggctaca taccagggac tgcggccacc
     4261 gccgcaaggg caccgaggag gagtacgcca ggaaggagag gaaacagcgc aaggacaacc
     4321 acatctcacc tggagagcag attctgatcc tcgaggtaca ggttgaggac gcccaggagt
     4381 tcatggacgt actcgaggta aggggagtcg aggtcggaga tcagcagatg ctgcgttcca
     4441 taagctccgt tgtccccaac gcgatcgcgg ccttcctgct acccctgcgc gacacaacgg
     4501 ggaagacgtc gcact
//
LOCUS       DASTKM010000063         5430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_188442, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000063 DASTKM010000000
VERSION     DASTKM010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188442"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..839)
                     /locus_tag="VFQ10_02380"
     CDS             complement(12..839)
                     /locus_tag="VFQ10_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9964219.1"
                     /translation="MADDPVSRGTLRVEGQELSYLHAGEGSPLLLLHGTYWSRVWAPV
                     IPRLAQDHAVFALDYPGFGRSGGRLTVENATVPRLAELILRAADALRVGEAFYLAGHD
                     IGGAVAQHITAFNQDRVPKLALINSVLYDSWPVPRVARFRDPEVARSVTPEQLVELRR
                     EALRGTVERCLSPGEEEDYLSPWRSEDATRSWTALAAAADARYTMELVEPLKRSGLPI
                     LLVWGEEDSAQPIDYARRFAREFPQARLVAVPGAGHIPMEDDPQMVGATLAGFFAEA"
     gene            complement(1048..1263)
                     /locus_tag="VFQ10_02385"
     CDS             complement(1048..1263)
                     /locus_tag="VFQ10_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9964220.1"
                     /translation="MDRVEDISREKLKEMMDRGDDFVLVETLDEEHYEFAHLPGAINL
                     PPGQTDRAQELIPDKGAEVVVYCASPP"
     gene            1638..3068
                     /locus_tag="VFQ10_02390"
     CDS             1638..3068
                     /locus_tag="VFQ10_02390"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9964221.1"
                     /translation="MTASCDLVVIGAGSGGITAARFAARAGARVVLIEKDRIGGDCTW
                     TGCVPSKALLKVARVAHQARNASGFGLSATTVGPVDLREVMDYVRRSISEVYRFETPE
                     VLRREGVDVRIGRARFEDANTLAVASAEGERTITAKKILLCTGARPNIPNLQGLSDVP
                     YLTSEQVYDLDALPTHLLVVGGGPVGVEMAQAFRRLGSRVSLFQGPARLLPRDEPEAA
                     ETLARILRDEGVGLHLGARVTSVALGDGGVLVRTRVGEARGDALLVATGRTPNVDTMD
                     LPHAGVRFTEGGVSVDGSLRTNVKHIYAAGDVVGGPQFTHYAGYQAFIAARNALFPGA
                     SKGLAESVPWTTFTDPEIAHAGLTEAEARNKYRNGDVGVRFVPMERVDRAVTESDTEG
                     FMKVVHKTNGTVLGATVVAERAGEAVHEWALAISNKLTMSDLASTIHVYPTYSIANQQ
                     LASTYSVESFLKSKAGKVLKKLGGLR"
     gene            complement(3216..3650)
                     /locus_tag="VFQ10_02395"
     CDS             complement(3216..3650)
                     /locus_tag="VFQ10_02395"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9964222.1"
                     /translation="MSEENKAVVHRFNELIGEFFRTGDADALDEVLAPDFVYHQPGTP
                     PDLESFKQFLPMFRAAFPDMRYTVEDMIAEGDKVVDRLTWQATHQGELMGISPTGNTV
                     EVTEMHISRIAEGKIVERWGQPDMLCLMQQLGVVPPPGQSGS"
     gene            complement(4096..4986)
                     /locus_tag="VFQ10_02400"
     CDS             complement(4096..4986)
                     /locus_tag="VFQ10_02400"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HET9964223.1"
                     /translation="MADGQNIERLILVCGATGKQGGAVARSLLDRGFQVRALSRDPQK
                     PEARALAERGAEVVQGDMEERSTMDQVLVEGIYGVFSVQNFWETGYDREVQQGKTVAD
                     AATAAGVEHLVYSSVGSAHRETGIPHFESKLKVEEHVRASGVPYTILRPAFFMQNWEW
                     MREPILGGTLAQPLDPDKPFQHVAVEDVGAFAAIAFDHPDEWIGREVDVAGDEQTMPE
                     IAQTFGAVIGSEVSYYQVPWDQFEEQMGEEAAVMYRWFNDVGYEADIAALRQEYPELT
                     TFERYLRSHGWEGAEVPAGA"
     gene            complement(5152..>5430)
                     /locus_tag="VFQ10_02405"
     CDS             complement(5152..>5430)
                     /locus_tag="VFQ10_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET9964224.1"
                     /translation="SAKEFKEEDHEVTVIFDGAGTKWVGELSDPEHKYNKHFESVKDE
                     VEGACAYCARAFGVKEDVQESGVELMGEFEGHPSLAKLVSEGYQVITF"
BASE COUNT         1050 a         1712 c         1565 g         1103 t
ORIGIN      
        1 cccggtcaca gttatgcctc agcgaagaag ccagccagcg tagctccgac catttgtggg
       61 tcgtcctcca ttggaatgtg acccgctccg ggcaccgcta cgaggcgtgc ctgaggaaac
      121 tctcgagcga agcggcgagc atagtcgatg ggttgagcgc tatcctcttc gccccatacc
      181 agcagtatgg gtaagcccga cctctttagc ggctccacta attccatcgt gtagcgtgca
      241 tctgcggcag cggccagcgc cgtccaggag cgagtggcat cctccgatct ccacggtgag
      301 aggtagtctt cttcttcacc gggcgacaaa catcgctcta ctgtcccgcg aagcgcctca
      361 cgacgaagct cgacgagctg ctcgggggtg acgctcctcg ccacctccgg gtctcgaaag
      421 cgcgctacgc ggggtacggg ccacgaatcg tagagcacgc tgtttatcag tgccagcttc
      481 ggcacacgat cctgattgaa cgccgtaata tgctgggcta cggcaccacc gatgtcatga
      541 ccggccaaat agaaggcctc cccaacccgc agcgcatctg cggcgcgcag aattagctcg
      601 gccaaccgcg gtaccgtggc gttctctacg gtaagccggc ctccagaacg gccaaagcca
      661 ggataatcga gcgcgaacac cgcgtgatct tgcgccaatc tcggtatcac cggcgcccac
      721 acgcggctcc agtaagtgcc atggagtaac agaagcggag atccttcacc ggcatgaagg
      781 taactgagct cctgtccttc aactctcaag gtacctcggg agactggatc atcggccatg
      841 gttatctcct gttgtgcatt ggtcgcaagg gggccagagc tccgaaaggg gatcccggcg
      901 agcgctggac ggtctctact atctctccga gcgctctcct tcgaccggca ggtcgtgtct
      961 catccagtcc cgcttacccc ctgcgtaatg gcagacattc tcgtagccca tggccaccag
     1021 ctcccgggcg gcctcctcgg acttcgttca tggcggactc gcgcagtaga cgacgacctc
     1081 ggcccccttg tcggggatca actcctgtgc ccggtcggtt tgccccggcg gtaggttgat
     1141 cgcgcctggc aggtgggcaa attcgtaatg ttcctcgtcg agggtctcga cgagcacgaa
     1201 gtcatccccg cgatccatca tctctttgag tttctcccta gagatgtctt ctaccctgtc
     1261 cacttatcgc ctccttacgg ctactcgccg tatagccaac ctctttccct aacgcgctag
     1321 ggactccttt tccagccggt gacacactta aacaaaggag aaccggaagg cctaaactcc
     1381 aaggacactg acccgcaagg tctttcgtca atgctgataa aggtcctcta tccattgccc
     1441 agaaacgctt ctgatcacat actacgtaga gcgggacgat taggttgggg tactactgcc
     1501 ccggctctct tctggcttgc tgctcgatag aattactgtg ctttgaggcg cccatacgct
     1561 accaaccccg gcttctcaac ccatctccgt gggttcgtct tgtgtgcgcg aagattatcc
     1621 gcagaaagga gtctgatgtg acggcgtctt gcgatctcgt ggtcatcggc gccgggtcgg
     1681 gcggcattac cgccgcgcgg ttcgcggccc gagccggcgc gcgggtggtg ctcatagaga
     1741 aggatcgcat cggcggcgac tgtacctgga ccggttgcgt gcccagcaag gccctgctca
     1801 aggtagccag ggtagcgcac caggcacgca acgcttccgg tttcggtttg agcgcgacca
     1861 ccgtggggcc ggtggacctc agagaggtga tggactatgt ccggcggtcc atctctgagg
     1921 tgtatcggtt cgaaaccccc gaggtcttac gccgagaggg cgtggacgtg cggataggcc
     1981 gggcaaggtt cgaggacgcg aacacgctcg ctgttgcctc cgccgaaggc gagaggacca
     2041 tcactgcaaa gaagatcctg ctctgcaccg gcgcgcgccc gaacatcccg aacttgcagg
     2101 gcctctcgga cgtgccgtac ctcacctccg agcaagttta tgatctggac gcgctgccca
     2161 cgcacctgct ggtggtgggc ggcgggccgg ttggcgtcga gatggcgcag gccttccgac
     2221 ggttgggctc gcgggtcagc ctgttccagg ggccggctcg tctgcttccc agggacgaac
     2281 ccgaggctgc agagacgctg gcgcggatac tgcgcgacga gggcgtcgga ctgcacctcg
     2341 gcgcgcgcgt gacaagcgtc gcgctgggcg acgggggtgt ccttgtgcgc accagggtgg
     2401 gcgaggcgag aggcgacgct ctgctcgttg caaccgggcg tacacctaac gtcgacacca
     2461 tggatctgcc gcacgcaggc gtgaggttca cagagggtgg tgtctcggtc gacggctccc
     2521 tgcgcaccaa cgtaaagcac atctacgcgg cgggcgacgt ggtcggcgga ccgcagttca
     2581 cccactacgc gggttaccag gcgttcatcg ctgcccgcaa cgcactgttc cccggcgcgt
     2641 ccaaggggct ggcggaatcg gtgccatgga cgaccttcac agatcctgag atcgcgcacg
     2701 ccggactcac cgaagccgag gcgcgcaaca aataccgtaa cggggatgtc ggcgtgcgct
     2761 tcgtgccaat ggagcgcgtg gaccgcgccg tgaccgagag cgacaccgag ggcttcatga
     2821 aggtggtgca caagacgaac ggcaccgtgc tcggcgcgac cgtcgtggcc gagcgggcag
     2881 gtgaggccgt ccacgagtgg gccctggcga tatcgaataa actgacgatg agcgacctag
     2941 caagcaccat ccacgtgtac cctacctact cgatcgccaa ccagcagctg gcgtcgacct
     3001 actcggtcga gtcgttcctc aaaagtaagg cgggcaaggt cttaaagaag ctgggaggcc
     3061 taaggtagca atgggttagg aaaccttgca cataaagtcg gcatttggag gtactttttg
     3121 tgcaactcgg atttctgcac aaaaagtctc gcatagagaa caccggggcg caacagcccc
     3181 ggctccatga ccgcgtggcg aatggattat agcgattaag atccgctctg ccccggcgga
     3241 gggacgacac ctagctgctg catcaagcat agcatgtccg gttggcccca acgctccacg
     3301 atcttcccct cggcgatgcg gctaatgtgc atctccgtca cctcaaccgt attgccggtc
     3361 ggggagatgc ccatcagctc cccttggtga gtggcttgcc acgtgagtcg gtccactacc
     3421 ttatctccct cggcgatcat atcctcgact gtatagcgca tgtcggggaa ggcggcgcgg
     3481 aacatgggga ggaattgttt gaagctttcg aggtcgggtg gtgtccccgg ctggtgatat
     3541 acgaagtctg gggcgagaac ctcatcgagg gcgtcggcgt cacccgtgcg gaagaattca
     3601 cctatgagtt cgttgaaccg atggacaacg gccttgttct cttccgacat agctaccctc
     3661 ctttccccgc ttccccaagc gagttcaacc cttgcctacc ggtatgatgc taccgaccga
     3721 agctcggcgc atccgcaaag tcttattggt gtgctacgaa gactcctgct gccacctctt
     3781 agcccaagct aggcttgggc ggcgttacgt aaagctactc ttcgtgctct tcgcccaccc
     3841 ctgacttttt gtgcagagcc ccaacttttt gtgcagcagc taccctatgt gcgaggccgg
     3901 gctacaggga catacgaata tgctcctggg tcaccggccg ccgacactca ggtaggatta
     3961 caaatgggtc aagattccgg ctaccgtctc aaccgaaggg tgacgctcgt cccgtctaga
     4021 tccgattctc cagcaattat ctgcttgatg cgggctccta tgacacctac gcaaaagcga
     4081 taattgccgt acagttcaag cccctgcagg aacctcggca ccctcccatc cgtggctgcg
     4141 gaggtaccgc tcaaaggtgg taagctcggg gtactcttga cgcaaggcag cgatatctgc
     4201 ttcgtagcct acgtcgttga accacctgta catgacggcc gcctcttcgc ccatctgctc
     4261 ctcgaactgg tcccagggca cctggtagta gctaacctca ctgcctatga cggctccgaa
     4321 ggtctgagct atctcgggca tggtctgttc gtccccggca acatccacct ctcgcccgat
     4381 ccactcgtca gggtgatcga aggctatggc ggcgaaggca ccgacgtcct cgaccgccac
     4441 gtgctggaac ggtttatccg gatcgagcgg ttgggccagg gtacctccga ggatgggttc
     4501 gcgcatccac tcccagttct gcatgaagaa cgccggacgc agaatcgtgt aaggtacgcc
     4561 actggcgcgc acgtgctctt ccaccttcaa cttgctttcg aagtgtggga tgcccgtctc
     4621 cctgtgcgcg ctgccgaccg agctgtagac gaggtgctcc acacctgcag ccgtcgccgc
     4681 atcagcgacc gtcttgccct gctgcacctc acggtcgtag ccggtctccc agaagttctg
     4741 cacagagaaa acgccgtaga tgccttcgac gagcacttgg tccatagtgg agcgctcttc
     4801 catatcaccc tggacaacct cggcgcctcg ctcagctagg gcccgcgctt ccggcttctg
     4861 cgggtcacgg gagagtgcgc gaacctgaaa gcctcgatca agaaggcttc gcgccaccgc
     4921 acctccctgc ttgccggtcg ctccgcacac cagaatcaag cgttctatat tttgcccatc
     4981 agccatatcc cttgctcctt ggttctgtgg taatcaaaac ctcatccgag tatagcttcg
     5041 agaaaagggc gactgaggcg ggaatacaac ccgcttcaag tagacaattg ccaattcttc
     5101 cacatggcct gccagagcca ccaggagacg ttccaacgct cccatggtgg gtcaaaatgt
     5161 gatcacctga taaccttcgg acaccagctt ggcaaggctg ggatgtcctt caaattcacc
     5221 catgagttcg acgccgctct cttggacatc ctctttgaca ccgaaggccc tcgcgcaata
     5281 ggcacaagcg ccctcgacct catccttcac cgactcgaaa tgcttgttgt acttgtgctc
     5341 cggatcggag agttcgccga cccacttggt gcctgccccg tcgaagatga cggtcacttc
     5401 gtggtcttct tccttgaact ccttggcgga
//
LOCUS       DASTKM010000064        14065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_12374, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000064 DASTKM010000000
VERSION     DASTKM010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14065
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12374"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..129
                     /locus_tag="VFQ10_02410"
     tRNA            57..129
                     /locus_tag="VFQ10_02410"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:90..92,aa:Trp,seq:cca)
     gene            160..408
                     /gene="secE"
                     /locus_tag="VFQ10_02415"
     CDS             160..408
                     /gene="secE"
                     /locus_tag="VFQ10_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET9964225.1"
                     /translation="MGKKRASAGSNKKQPQNKGGGFLAGPRRFARDVRGELRRVSWPD
                     RDQLRQSTAVVLIIVIVLALYVAAVDVVFQSLVRLVFL"
     gene            441..971
                     /gene="nusG"
                     /locus_tag="VFQ10_02420"
     CDS             441..971
                     /gene="nusG"
                     /locus_tag="VFQ10_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET9964226.1"
                     /translation="MKKWYVVNTYSGHENKVRTTLERRIDSMGLERNFGEIRIPTESV
                     IEIKDGKKVPTIQRQFPGYVLVNMDMNDTTWQLVRQTPGVTQIVGAGEKPLALSRAEV
                     ERLLHPGEVETREKVKTTVDYQIGETVKVIGGPLSDFTGSISDINVDQSRLKVLVSIF
                     GRETPVELSFNQVAKL"
     gene            986..1423
                     /gene="rplK"
                     /locus_tag="VFQ10_02425"
     CDS             986..1423
                     /gene="rplK"
                     /locus_tag="VFQ10_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET9964227.1"
                     /translation="MGRGRGRRVARKLKLQITGGQANPAPPVGPALGQAQVNIGEFVR
                     QFNDATRDQLGQIVPVEITVYEDRSFTFITKTSPAAFLLKQAAGLDKGSGEPNRMKVG
                     TVSRAQVEEIARTKMPDLNAADIEGAAKIVEGTARSMGITVDG"
     gene            1416..2105
                     /gene="rplA"
                     /locus_tag="VFQ10_02430"
     CDS             1416..2105
                     /gene="rplA"
                     /locus_tag="VFQ10_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET9964228.1"
                     /translation="MGRKLLEQKSRIDPEQRYTPREALELLKGLEGANFDESVEAHVH
                     LNVDPRRADQMVRGTLMLPNGTGKTRRVAVFAEGEKAREAEESGADVVGSDDLAARIK
                     DEGFMDFDVAVATPDQMRAVGQLGPVLGPRGLMPNPKSGTVTQDVGRAVEEIKRGKIE
                     YRVDRYGIIHGVIGKKSFDLDSLVENYYALREELQRARPAPVKGRYFKSVALSTTMGP
                     SVKVDPAVERD"
     misc_feature    2120..2253
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2262..2789
                     /gene="rplJ"
                     /locus_tag="VFQ10_02435"
     CDS             2262..2789
                     /gene="rplJ"
                     /locus_tag="VFQ10_02435"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET9964229.1"
                     /translation="MNREEKVRVIESLTAKLRVGSAVLVDYQGMDVARSTDLRRRSRE
                     SGVDFVVAKNTLTKRAADEAGVEGISEFLVGPTALAFSEDPVASAKLMAEFADQVESF
                     VLKGGLLEGGRVLGEAEVVALSKLPGREQLLAQIVGGISSPLTGLVTVLNNTVQGLVV
                     ALGQIAEQKQSAEQS"
     gene            2816..3199
                     /gene="rplL"
                     /locus_tag="VFQ10_02440"
     CDS             2816..3199
                     /gene="rplL"
                     /locus_tag="VFQ10_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET9964230.1"
                     /translation="MALNKEELMESIENMTVLELSELVKALEERFGVSATAVAAAPAA
                     GAAGDGAAAAEEEQTEFDVVLQGPGEKKIQVIKVVRAATGLGLKEAKALVDEAPNPVK
                     EALSREDADALKTQLEEAGASVELK"
     gene            3542..6859
                     /locus_tag="VFQ10_02445"
     CDS             3542..6859
                     /locus_tag="VFQ10_02445"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET9964231.1"
                     /translation="MPDLVEIQRNSFQKFLNEGIQKTFTEISPIEDYTGSYAVEFGEG
                     RFEDPPVSVEECMSKDKTYAAPLFVKVRFIVKETGEVREQDVFMGDFPLMTDSGTFVI
                     NGTERVVVTQLVRSPGAYIMEPKDPTKQVLTASLMPARGSWLEFEVEAKGYVSVRIDR
                     KRKLPVTVLLKALGMGGPEEILSRFGDSWLVRNTLERDDTPSREEALLEVFRRQRPGE
                     PVNIENAQNLVDGLFFDAKRYDLSEVGRYKVNKKLKLDVPPDTQTLTIEDIEALLRRL
                     IETSEEVAEDEEFGRVNYERIRHKLDEYEHFGNRRLRTVGELVQEAFRIGMYRMERVV
                     RERMTTQDEDSITPQSVVNVKPVSSAIKEFFGSSQLSQFMDQTNTLSGLSHRRRLSAM
                     GAGGLSRERAPIEVRDVHPTHYGRMCPIETPEGPNIGLIGQLSTFATVDDYGFVQTPY
                     RKVEKGKLTDEIEYLSADEEEEFTIAQANTPIDPKTGKISEDAQILARRKGGDVATVA
                     AEEVDYVDVAPLQTVSVGTALIPFLEHDDANRALMGANMQKQAVPLLRSEAPYVGTGM
                     EFRAATDTGDVLLAHHAGTVERVVSDRITVRRSGAGGELDEYYLRKFARSNQGTCVNQ
                     RPLVKEGDEVEAGTIMADGSSTDQGELALGKNLLVAFMPWEGFNFEDAIIISERIVKD
                     DTLSSIHIEEYEVQARDTKLGPEEITRDIPNASDDVLMNLDVDGIIRIGAEVGSGDVL
                     VGKVTPKGESEPTPEEKLLRAIFGEKAREVKDSSLKVPHGEGGVVIDVKRFSRDGGDE
                     LQPGVNEMVRVFVAKKRKIAEGDKLAGRHGNKGVISKIVPEEDMPFMEDGRPVDVILS
                     PLGVPSRMNIGQILETHLGWAASQGLGEDGGEPVFVSTPVFSGAEVEDVNEAIENVRN
                     NGGAGTGMGRGGKVTLYDGRTGEPFDGRITVGYMYILKLLHLVDDKIHARSTGPYSLV
                     TQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQELLTIKSDDRVGRVKSYESIVKGE
                     NIPEPGVPASFKVLLKEMQSLGLSVKPVYNAEAAAEDQDRRDWDEAARALGINLSRDE
                     PTGNLEDSPGAFGRGGI"
     gene            6863..10744
                     /locus_tag="VFQ10_02450"
     CDS             6863..10744
                     /locus_tag="VFQ10_02450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET9964232.1"
                     /translation="MQGLERDIDINKLEKISIGLASADEIREWSRGEVTKPETINYRT
                     LRPEKDGLFDERIFGPSKDWECYCGKYKRIRFKGIICERCGVEVTRARVRRDRMGHIE
                     LAAPVAHVWFVKGVPSRMGYLLDISPKELDRVLYFASSIVTWVDREERANDIDLLRGR
                     VEDEIQQLELDRDEEIDQIQALRTKRESVIKGESSPDELTEEYAEIPEDERKAVITKV
                     YKEAEEAVADIGRSVAEQIGLVRRAWEEFQTLEPRQIVDDEELFREMKDRFGDEYGYG
                     VYFRGGMGAEAIRDLIGQVDLKNEAQDLRDIVASSRGQKRQKAIKRLKVVDAFRVSGN
                     SPEWMILEAIPVLPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLRRLLDLGAPDV
                     IVNNEKRMLQEAVDALFDNGRRGRAVTGAGNRALKSLSDMLKGKQGRFRQNLLGKRVD
                     YSGRSVIVVGPGLKMHQCGLPRLMAVELFKPFVMKVLVDRALAPNIRSAKQMVERLRP
                     EVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVEGKAIQVHPLVCSAFNADFDGD
                     QMAVHVPLSPEAQAEARVLMLSTQNILLPANGFPIAVPSQDMVLGLYYITYGPEDFDE
                     AEVKALLSSYDEAEAAYKEGTLKIHDKVIARRGGERIETTLGRVLFNENVEQTLRDYL
                     GDAYDPESYPYINHTLKKGEIKDLVAGYVARYPTELVSQLLDTVKRVGFHTATRAGIS
                     VGKNDIVVPEQKEEILERYQGMVDEIEDQWDQGFISDDEREERVITLWTEAKNEVEQA
                     MKDNLWRLNPIYMMADSGARGNYNNFTQLAGMRGLMTNTKGEIIEEPVKSNFMEGLSV
                     LEYFTSTHGARKGQADTALRTADSGYLTRRLVDVSQDVVVNEEDCGSDGYVDLPLYLE
                     GGRGDANDSVAARFLARDLVNPQTGEVVASAGEDITPGLLATWRKMLPRETLVPVRTP
                     SKCESPHGVCQTCYGRALSTYRPVDVGEAVGIIAAQSIGEPGTQLTMRTFHTGGIAGE
                     DITAGLPRVVELFEARHPKAEATLAEVDGIVSLEEADSDRQIKVVITAPDGEERSYTV
                     ERQLLRPEFVEGAEVRANTPITEGSIYPHAILENDLHYGRGSTTTERYLVDEVQKVYR
                     AQGVDIHDKHIEVIVRQMLRKVIVDDPGDTKLLPEQVADRFTVLAENARVEEEEGRPA
                     TYHTVLMGITKASLATDSFLSAASFQETARVLTDAAIEGKVDSLSGLKENVIIGKLIP
                     AATGLPRYRQLTVAVEGYRTQDVDELDIAAS"
     gene            10924..11292
                     /gene="rpsL"
                     /locus_tag="VFQ10_02455"
     CDS             10924..11292
                     /gene="rpsL"
                     /locus_tag="VFQ10_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET9964233.1"
                     /translation="MPTTNQLVRNEREKQTKKAATPALQGSPQKRGVCTRVSTTTPRK
                     PNSALRKVARVRLVNGQEVTAYVPGEGHNLQEHSVVLVRGGQVKDLPGVRYKVVRGAL
                     DTLGVNNRKQGRSKYGTKKS"
     gene            11304..11774
                     /gene="rpsG"
                     /locus_tag="VFQ10_02460"
     CDS             11304..11774
                     /gene="rpsG"
                     /locus_tag="VFQ10_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET9964234.1"
                     /translation="MPRRAAPPKKKIAADPVYGSVLVQQLINKIMLDGKKSTSESIVY
                     DALSYIEERTGTSPVQVLNNALDNIRPRLEVKSRRVGGATYQVPVEVDARRANTLALR
                     WMVGFARARREKTMGRRLAGEILDAKDNIGSSVKRKEDTHRMAEANRAFAHYRW"
     gene            11787..13934
                     /gene="fusA"
                     /locus_tag="VFQ10_02465"
     CDS             11787..13934
                     /gene="fusA"
                     /locus_tag="VFQ10_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9964235.1"
                     /translation="MAVQVSRKIPLKRVRNIGIMAHIDAGKTTTTERILLYTGLTHKL
                     GEVHDGNAVMDWMAQERERGITITSAATTVFWGGIEGSSKNGKGTREGVHSRIADQHR
                     INIIDTPGHVDFTVEVERSLRVLDGAIALFDSVAGVEPQSETVWRQADKYGVPRIAFV
                     NKMDRIGADFYKAVGTMVDRLGANAVPVQLPIGAESEFQGIVDLVEMRAIVYTDDLGA
                     TWEVTDIPEDIGALAEEYREKLLEAVADQDEELMVMYLEGEEIEPDQIRAAIRQATLD
                     IRMTPVFVGSAFKNKGVQPLLDGVVDYLPSPLDVPPVEGRTLDGEEITREADENGPLA
                     ALAFKVQADPHVGKLTYVRVYSGTLKAGSYVMNTTKGRRERVGRLLQLHANSREQRDE
                     VYAGELVAAIGLSDTGTGDTLVSADDEEQIVLEEMIFPEPVIRQAIEPKTKADQEKLS
                     VALQRLAEEDPTFTVRSDEETGQTIVAGMGELHLEIIVDRLLREFRVDANIGRPQVAY
                     RETIRKRVEGIEGRFVRQTGGRGQYGHAVISAEHNDEAGYEFDNKIVGGVIPREYIPS
                     VDKGIREALDSGVIAGYPVVDVRVELVDGSYHEVDSSEMAFQIAGSMAIKEALKRANP
                     VLLEPVMDVEVVVPEEFMGDVMGDLSARRGHIQGMDSRGGGQVIRAMVPLSEMFGYAT
                     TVRSKTQGRATFTMQFDHYAEVPKSIAAEIAER"
     gene            13941..>14065
                     /locus_tag="VFQ10_02470"
     CDS             13941..>14065
                     /locus_tag="VFQ10_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9964236.1"
                     /translation="MSRGRFERTKPHLNVGTIGHVDHGKTTLTAAITRVLAKEVP"
BASE COUNT         2958 a         4220 c         4644 g         2243 t
ORIGIN      
        1 ccgtggtgcc gccatcatac ggcgcaccgg gagacccggt aaggatcgtc agacagaggg
       61 cagtagctca gttggtagag cagcggtctc caaaaccgca ggtcgggggt tcgagtccct
      121 cctgccctgc ttcgagaggc aaagccatgc gaggacacaa tgggtaagaa gcgcgcatcc
      181 gcggggtcga acaagaagca gccgcagaac aagggcggcg gctttcttgc tggaccgagg
      241 aggtttgcca gggacgtgcg gggtgagctc aggcgggtca gctggcccga ccgtgaccag
      301 ctcaggcaaa gcacggccgt ggtgctcatc atcgtgatcg tcctggcgct gtacgtcgcc
      361 gccgttgacg tcgtgttcca gagcctggtg cgcctggtct tcttatagat cttcctatta
      421 ggtattagga gcgtttcgtt ttgaagaagt ggtacgtcgt aaatacatac tcgggtcacg
      481 agaacaaggt gcgcacgacg ctggagcgca ggatcgattc catgggcctc gagcggaact
      541 tcggtgagat ccgcatcccg accgagagcg tcatcgagat caaggacggc aagaaagtcc
      601 ccacgatcca gcgccagttc cccggctacg tcctcgtgaa catggacatg aacgacacga
      661 cctggcagct cgtgcgccag acgccgggag tcacccagat cgtgggcgcc ggggagaagc
      721 cgctcgctct cagccgggcc gaggtggaga ggctcctgca tccaggcgag gtcgagacgc
      781 gcgagaaggt caagacgacg gtggattacc agatcgggga gacggtcaag gttattgggg
      841 gaccgctctc ggacttcacg ggttcgatct cggatatcaa cgtggatcag tcgaggttga
      901 aagtgctcgt ctccatcttc gggcgcgaga cgcccgtgga gctctctttc aaccaggtag
      961 cgaagctcta aggagaggat ttttcatggg aagaggacgc gggcgccgcg tagcgcgcaa
     1021 gttgaagttg cagatcacgg gcgggcaggc gaacccggct ccgccggtgg gccctgccct
     1081 cggccaggcg caggtgaaca tcggcgagtt cgtgcggcag ttcaacgacg ccacgcgcga
     1141 ccagttgggc cagatcgtac ccgtggagat cacggtctac gaggaccgct ctttcacttt
     1201 cataaccaag acatctccgg cggcgttctt gctgaagcag gctgctggac tcgacaaggg
     1261 cagcggcgag ccaaacagga tgaaggtcgg aacggtgagc agggcccagg tcgaggagat
     1321 agcacgtacc aagatgcctg acctcaacgc cgcggacatc gagggcgcag cgaagatcgt
     1381 ggagggtacg gccaggagca tggggataac ggtcgatggg taggaagctg ctcgaacaga
     1441 agtcgaggat agatccggaa cagcgctaca cgccgcgcga ggcgctagag ctactgaagg
     1501 gcctcgaagg cgcgaacttc gacgagagcg tcgaggccca cgtccacctg aacgtggacc
     1561 ccaggcgcgc cgaccagatg gtgcgtggca cgctgatgct gccgaacggc acgggcaaga
     1621 cgcgcagggt cgctgtcttc gccgagggcg agaaggccag ggaggccgag gagtcagggg
     1681 ccgacgtggt cggctcggac gacctcgcgg cccgcatcaa ggacgagggc tttatggact
     1741 tcgacgtagc cgtcgcaacc ccggatcaga tgagggccgt gggccagctc ggtcccgtcc
     1801 tcggtccgcg cgggctcatg ccgaacccga agagtggcac agtgacgcag gacgtaggca
     1861 gggccgtcga ggagatcaaa cgcggcaaga tcgagtaccg cgttgaccgc tacggcatca
     1921 tccacggcgt aataggaaag aagtccttcg atctggatag cctggtcgaa aactactacg
     1981 cgctgcgcga ggagctgcag cgggcccgtc ccgctcccgt aaaggggcgc tacttcaagt
     2041 ccgtggcact gagcacgacg atgggaccct ccgtgaaggt tgaccccgcg gtggagagag
     2101 attagtatca cgcacggact ttagtcaata gagcagacca gagacagccg gggtccggaa
     2161 ggacttaatc atccggccga ggttgcggaa agctttcgag ggatctcttt gccccgagcg
     2221 cctctgcggc ctcggggctt tctagtcgca gaggagtcga attgaacagg gaagagaaag
     2281 tgcgggtgat cgagagcctc accgccaagc tgcgggtggg ctcagcggtg ctcgtcgact
     2341 accagggcat ggacgttgcc aggagcacag acctccgtcg ccgtagccgc gagtccggcg
     2401 tcgacttcgt ggtcgcgaag aacaccctga ccaagagggc agcggacgag gctggggtag
     2461 aggggatctc ggagttcctc gtcgggccca cggccctcgc cttctcggag gatcccgtcg
     2521 ccagcgcgaa gctgatggcc gagttcgccg atcaggtgga gtctttcgtg ctcaaaggcg
     2581 gcctcctgga aggcggacgc gtgctcggcg aggccgaggt ggtggccttg agcaagctgc
     2641 cgggccgcga gcagctgctc gcgcagatag tcggcggcat ctcctccccg cttacggggc
     2701 tggtcacggt gctcaacaac accgtccagg gtctggtagt ggcgctcggc cagatcgccg
     2761 agcagaagca gagcgccgaa cagtcgtagt cgaacaaaga taggaggacc cagcgatggc
     2821 actcaacaaa gaagaactga tggagtcgat cgagaacatg accgttctcg agctctcgga
     2881 actggtgaag gccctggagg agcgcttcgg cgtctccgcc acagccgtag cagcagcccc
     2941 cgccgctggc gcggccggcg atggcgctgc cgccgccgaa gaggagcaga ccgagttcga
     3001 cgtcgtgctc cagggcccag gcgagaagaa gatccaggtc ataaaggtcg tgagggcagc
     3061 gacaggcctc ggcctgaagg aggcgaaggc cctcgtcgac gaggccccca accccgtcaa
     3121 ggaggccctg agccgcgagg acgccgacgc cctcaagacc cagctcgaag aggccggcgc
     3181 gtctgtggaa ctcaagtagg gctcgcccta cttgagttcc acgccggtca gcttgcacgt
     3241 gcgcaaagga ccttgctgac aagctgaggc cgttaggccg aagcgtgctg acatgccgct
     3301 cggcgcaagc gccgttcgtg gcatgccacg aagagctgtg ctacactccc ggcgccggtg
     3361 caggaccggc cacaaattat ttgcgattct gccttgactt gcccaggtat caagggtaag
     3421 atacccgttt gcgtgttcgg ccattcttca acgacccatg cggaggagag ctaacttggt
     3481 gagccctgtc gtgcgccgga aacggcaccc gtatgctcgg ttacagtcca cagaagttca
     3541 gttgccggat ctcgtcgaga tccagcggaa ttcttttcag aagtttctaa acgagggtat
     3601 ccagaagaca ttcacggaga tctcccctat agaggactac acaggctctt acgccgtgga
     3661 gttcggggag ggtcgcttcg aagaccctcc ggtctccgtc gaagagtgca tgtccaagga
     3721 caagacctat gcggcgccgc tgttcgtgaa ggtccgcttt atagtcaagg agaccggaga
     3781 ggtcagggag caggacgtct ttatgggcga tttcccgctc atgaccgact cggggacctt
     3841 cgtcatcaac gggaccgagc gggtcgtcgt cacgcagctc gtacggtcgc ctggcgccta
     3901 catcatggag ccgaaggacc ctaccaagca ggtcttgaca gcgagcttga tgcctgcgcg
     3961 cgggtcctgg ttggagttcg aggtcgaggc caagggctac gtctccgtgc gcatcgaccg
     4021 caagaggaag ctgcctgtca cggtgctgct caaggcacta gggatgggcg ggccagagga
     4081 gatactaagt aggttcggcg actcgtggct cgtgcgcaac accctggaaa gggacgacac
     4141 cccttccagg gaagaggccc tgctggaggt cttcaggcgg cagaggcctg gcgagccggt
     4201 caacatcgag aacgcccaga acctcgttga cgggctgttc ttcgacgcca agcgctacga
     4261 cctctccgag gtcgggcgct acaaggtcaa caagaagctc aagctcgacg tgccgcccga
     4321 tacccagacc ctgaccatcg aggacatcga ggccctcctg cgtcgcctca tcgagacctc
     4381 cgaggaggtc gccgaggacg aggagttcgg cagggtcaac tacgagcgca tccgccacaa
     4441 gctcgacgag tacgagcact tcggcaacag gcgcctgagg accgtagggg aactggtgca
     4501 ggaggcgttc aggatcggga tgtaccgcat ggagcgcgtg gtgcgcgagc ggatgacgac
     4561 ccaggacgag gattccataa ccccccagag cgtggtcaac gtcaagcccg tgagcagcgc
     4621 cataaaggag ttttttgggt cctcccagct ctcgcagttc atggaccaga ccaatacctt
     4681 gagcgggctc tcccacaggc gacgcctctc cgcgatgggg gctggcggtc tgagcaggga
     4741 gcgggcgccg atagaggtgc gcgacgtgca tcccacccac tacggcagga tgtgccccat
     4801 agagacgccg gagggcccga acatcgggct catcgggcag ttgtcgacgt tcgcgaccgt
     4861 agacgactac ggtttcgtac agacgcccta caggaaggtc gagaaaggga agctcacaga
     4921 cgagatcgag tacctctccg ccgacgagga ggaagagttc acgatcgccc aggcaaacac
     4981 ccccatagac cccaagacgg gcaagatctc cgaagacgcc cagatcctgg cccgcaggaa
     5041 gggcggagac gtggcgaccg tggccgccga ggaggtcgac tacgtagacg ttgcgccttt
     5101 gcagacggtc tccgtcggca cggctttgat cccgttcctc gagcacgacg acgccaacag
     5161 ggcgctcatg ggcgccaaca tgcagaaaca ggccgtgccg ctcctcagga gcgaggcccc
     5221 ttacgtcggg acggggatgg agttccgggc tgcgaccgac accggcgacg tgctccttgc
     5281 gcaccacgct ggcaccgtcg agcgggtcgt ctccgacagg ataacggtca ggcgtagcgg
     5341 agctggtggc gagctggacg aatactactt gaggaagttc gcccgctcca accaggggac
     5401 ctgtgtcaac cagcgcccgc tcgtcaagga gggcgatgag gtcgaggccg ggactatcat
     5461 ggccgacggc tcctccaccg accagggcga gctggccctg ggtaagaatt tgctcgtggc
     5521 tttcatgcct tgggaggggt tcaacttcga ggacgccatt atcatctccg agcggatcgt
     5581 caaggacgac accctctcct ccatacacat cgaggagtac gaggtgcagg cccgcgacac
     5641 aaagctaggg ccggaggaga tcacccgcga tatccccaac gcctcagacg acgtcctgat
     5701 gaacctggac gtggacggca tcatccgtat cggggccgag gtcggctccg gagacgtact
     5761 cgtcggcaag gtcaccccca agggcgagag cgagccgacg cccgaggaga agctgctgcg
     5821 cgcgatcttc ggcgagaagg cgcgtgaggt caaggattct tccctcaagg taccgcacgg
     5881 cgagggcggc gtcgttatcg acgtcaagcg cttctcgcgt gacgggggcg acgagctcca
     5941 gccaggcgtc aacgagatgg tcagggtctt cgtcgccaaa aagcgcaaga tcgctgaggg
     6001 cgacaagctc gccgggcgtc acggcaacaa gggcgtcatc tccaagatcg tgcccgagga
     6061 ggacatgccg ttcatggagg atggtcgtcc tgtcgatgtg atcctctcac ccttgggcgt
     6121 tccgagccgt atgaacatcg gccagatcct ggagacccac ctcgggtggg ccgccagcca
     6181 gggcctcggg gaggacggcg gcgagcccgt cttcgtctcg acgcccgtgt tctccggcgc
     6241 tgaggtcgag gatgtcaacg aagctataga gaacgtgcgc aacaacggtg gtgctggaac
     6301 gggcatgggc cgcggtggca aggtcacgct ctatgacggc aggacaggag agcctttcga
     6361 cggcaggatc accgtcggtt acatgtacat actcaagctc ttgcacctgg tggacgacaa
     6421 gatccatgcc aggagcacag gtccttactc gctggtcacc cagcaaccgc ttggcggcaa
     6481 ggcccagttc ggcggccagc ggttcggaga gatggaggtg tgggcgctcg aggcctacgg
     6541 cgcggcgcac accctgcagg agctgcttac tataaagagc gacgacaggg tcggcagggt
     6601 caagagctac gagtcgatag tcaagggcga gaacatccct gagcctggtg tgccggcgag
     6661 tttcaaggtg ctgctcaagg agatgcagtc cttggggctc tccgtcaagc cggtctacaa
     6721 cgccgaggcc gcggccgagg atcaggaccg cagggattgg gacgaagccg cccgcgcgct
     6781 gggcatcaac ctcagccgcg acgaaccgac cggcaacctg gaggactcac cgggcgcctt
     6841 cggcagagga ggtatataga gcgtgcaggg actcgagcgc gacatagaca taaacaaact
     6901 ggagaagatc tccatcggcc tcgcctccgc cgatgagatc agggagtggt ccaggggcga
     6961 ggtcaccaag cccgagacca tcaattatcg tactctacgt cctgagaagg acggcctctt
     7021 cgacgagcgc atcttcggcc cttccaagga ctgggagtgc tactgcggca agtacaagag
     7081 gatccggttc aagggcatca tctgcgagag gtgcggggtc gaggtaaccc gcgcccgcgt
     7141 acgccgcgac aggatgggcc acatcgagct cgccgcccct gtggcgcacg tgtggttcgt
     7201 caagggcgtg cctagccgaa tgggatatct gctcgatatc agtcccaagg agctcgaccg
     7261 ggtactctac ttcgccagct ccatagttac gtgggtcgac cgtgaggagc gggccaacga
     7321 catagacctg ctgcgtggca gggtggagga tgagatacag cagctcgagc tggacaggga
     7381 cgaggagatc gaccagatcc aggccctgag gaccaagcgc gagagcgtga taaagggcga
     7441 gagctctccg gacgagctga ccgaggagta cgcggagata cccgaggacg agcgcaaggc
     7501 cgtcataacg aaggtataca aggaggccga ggaggccgtc gcggacatag gccgctcggt
     7561 tgccgagcag atagggctcg tcaggcgcgc ctgggaggag ttccagacgc tggagccgcg
     7621 ccagatcgtg gacgacgagg agctcttccg cgagatgaag gaccgtttcg gagacgagta
     7681 cggctacgga gtgtacttcc gcggcgggat gggcgccgag gccataaggg acctcatagg
     7741 acaggtcgac ctcaagaacg aggcccagga cctcagggac atcgttgcct cctccagagg
     7801 acagaagcgc cagaaggcga tcaaacgcct caaggtcgtc gacgccttcc gcgtgagcgg
     7861 caacagcccc gagtggatga tcctggaggc catccctgtg ctcccgccgg acctccgccc
     7921 gatggtccag ctcgacggcg gccgtttcgc cacctctgat ttgaacgacc tctaccgcag
     7981 ggtcataaac aggaacaaca ggcttcgtcg cctgctcgac ctcggcgctc ccgacgtgat
     8041 cgtgaacaac gagaagcgga tgctccagga ggccgtcgac gccctcttcg ataacgggcg
     8101 tcgcggacgc gccgtcacag gcgccggaaa cagggcgctc aagagcctct cggatatgct
     8161 caagggcaag cagggccgct tcaggcaaaa cttgctcggc aagcgcgtgg attattccgg
     8221 ccggtcggtc atcgtggtcg gccctggcct gaagatgcac cagtgcggcc tcccacggct
     8281 tatggccgtc gagctcttca agcctttcgt gatgaaggtc ctcgtagaca gggccctggc
     8341 gccgaacatt cgtagcgcca agcagatggt ggaaaggctc cgtccggaag tctgggacgt
     8401 cctggaggac gtcatcaagg agcaccccgt gttgctcaac cgcgcgccga cgctccaccg
     8461 ccttggcatc caggcgttcg agcccgtgct cgtggagggc aaggccatcc aggtccaccc
     8521 gctggtctgt tccgccttca acgcggactt cgacggcgac cagatggcgg tgcacgtacc
     8581 cctgagcccc gaggcgcagg cagaggcccg cgtcctcatg ctctccacgc agaacatact
     8641 tctgcccgcc aacgggttcc ccatcgccgt tccctcgcag gacatggtgc tcgggctcta
     8701 ctacataacc tacggccccg aggacttcga cgaggccgag gtcaaggcgc ttctctcctc
     8761 ttacgacgag gccgaggccg cgtacaagga ggggacgctc aagatccacg acaaggtcat
     8821 agcccgcagg ggcggcgaac ggatcgagac caccctgggg cgcgtccttt tcaacgagaa
     8881 cgtcgagcag accctaaggg actacctcgg cgacgcctac gaccccgagt cctatcccta
     8941 catcaaccac acattgaaga agggtgagat caaagacctc gtcgccgggt acgtggcccg
     9001 ttacccgacg gagctggtga gccagctcct cgacaccgtg aagagggtgg ggttccacac
     9061 ggccaccagg gccggcatct cggtcggcaa gaacgacatc gtcgtgcccg agcagaaaga
     9121 ggagatcctc gagcgctacc agggcatggt cgacgagatc gaggaccagt gggaccaggg
     9181 cttcatctcc gacgacgagc gggaagagcg cgtcataacc ctctggaccg aggccaagaa
     9241 cgaggtcgag caggcgatga aggacaacct ctggcgcctg aacccgatct acatgatggc
     9301 cgactcgggc gcccgcggca actacaacaa cttcacccag ctcgctggca tgcgcggcct
     9361 gatgacgaac acgaagggtg agatcatcga ggagcccgtg aagagcaact tcatggaggg
     9421 cctctcggtg ctggagtact tcacctcgac ccacggcgcg aggaagggtc aggccgacac
     9481 tgccctgcgt accgccgact cgggctacct gacacgccgc ctcgtcgacg tctcccagga
     9541 cgtcgtggtg aacgaagagg actgcggctc ggacggctac gtcgaccttc cgctctactt
     9601 ggaaggcggc aggggcgacg ctaacgattc ggtcgctgcc cgcttcctgg cccgagatct
     9661 ggttaacccc cagacgggag aggtcgtcgc cagcgcaggc gaggacatca ccccaggtct
     9721 ccttgccacc tggcgcaaga tgctgccgcg cgagacactc gttcccgtgc ggacgccctc
     9781 caagtgcgag agcccgcacg gcgtctgcca gacctgttac ggcagggcgc tttcgactta
     9841 caggcccgtc gacgttggcg aggccgtcgg catcatagcg gcccagtcta tcggggagcc
     9901 tggcacgcag ctcacgatgc gcaccttcca caccggcggc atagcaggcg aggacatcac
     9961 agcaggcctc cccagggtcg tcgagctctt cgaagcccgt catcccaagg ccgaggcaac
    10021 cctcgccgag gtcgacggta tcgtctccct ggaggaagca gactccgaca ggcagatcaa
    10081 ggtcgttatc accgcgcccg atggcgagga gaggtcgtac acggtcgagc gccagctcct
    10141 caggcccgag ttcgtggagg gcgccgaggt cagggcgaac accccgatca cggaagggtc
    10201 tatctacccg cacgccatcc tggagaacga cctccactac ggccgtggca gcaccacgac
    10261 cgagcgctac ctagtcgatg aggtgcagaa ggtctaccgc gcacagggcg tggacatcca
    10321 cgacaagcac atcgaggtca tcgtacgcca gatgctgcgc aaggtgatcg tggacgaccc
    10381 aggcgacacg aagctgctcc ccgagcaggt cgccgaccgc tttaccgtcc tggccgagaa
    10441 cgcacgcgtc gaggaggagg aaggccgccc agccacctac cacacggtcc tgatgggcat
    10501 aaccaaagcc tcgctcgcca ccgacagctt cctctctgcg gcctctttcc aggagacggc
    10561 acgcgtcctt accgacgctg ccatcgaggg caaggtggat agcctctcag gcctgaagga
    10621 gaacgtcatt atcgggaagc tcatacccgc agccacagga ctgccgcgtt acaggcagct
    10681 aaccgtggcc gtcgagggct acaggacgca ggacgtcgat gagcttgata tagcggcgtc
    10741 ttagccactc agcagttggg ccggggcgac tacgccccgg ccttttcacc ttgacataac
    10801 ggtcttctga gcctaaaata gttcgctgcg gttacgagtc gttaccaatg ggtagcaaag
    10861 actttgtgcc gtggcacgtg attctgtaag cccctttgca cggatagaga gagaggcgcg
    10921 gccatgccga cgaccaacca gcttgtgcgc aacgagcggg agaaacagac gaagaaggcg
    10981 gcgacgccgg cgctgcaggg gtctccccag aagcgcggcg tgtgcacgcg cgtctcgacg
    11041 accacgccga ggaagccgaa ctctgcgctg cgcaaggtgg ccagggtccg tctggtgaac
    11101 ggtcaggagg tcactgccta cgttcccggg gagggacaca atctgcagga gcactccgtc
    11161 gtgctcgtgc gtggcggaca ggtcaaggac ctgcccggtg tgcgttacaa ggtcgtgcgc
    11221 ggggcgctcg atactctcgg ggtcaacaac cgcaagcagg gccgttccaa gtacgggacc
    11281 aagaagtcgt aggaggatag gagatgccga ggcgagcagc gccaccaaag aagaagatag
    11341 ccgccgatcc cgtctacggg agcgtgctgg tgcagcagtt gatcaacaag atcatgctcg
    11401 acggtaagaa gagcacctcg gaaagtatcg tctacgacgc cctctcttac atcgaggagc
    11461 gcacgggcac gagcccggtc caggtactga acaacgccct ggataacatc aggccccgcc
    11521 tggaggtcaa gagccggcgc gtcggcggcg cgacctacca ggtgcccgtt gaggtcgatg
    11581 cccgcagggc caacacgctg gcgctgcgct ggatggtggg cttcgcccgc gccagacgcg
    11641 agaagaccat gggccgcagg ctggcagggg agatcctgga cgccaaagac aatatcggct
    11701 cgagcgtcaa gcgtaaggaa gacacgcatc ggatggcaga ggccaacagg gccttcgccc
    11761 attacaggtg gtgacgagga actaatatgg ctgtacaagt atccagaaag atcccgctaa
    11821 aacgggtcag aaatatcggc atcatggccc acatcgatgc tggaaagact acgacgacgg
    11881 agcgcatcct gctctatacg ggccttaccc ataagctggg cgaggtccac gacggtaacg
    11941 ccgtgatgga ctggatggcc caggagcgcg aacgcggcat taccataact tcagccgcca
    12001 cgaccgtgtt ctggggcggc atcgaaggct ctagcaagaa cggcaagggt acccgcgaag
    12061 gcgtccatag ccgcatagca gatcagcatc gtataaatat tatagacacg cccgggcacg
    12121 tggactttac tgtggaagtg gagcgcagtc ttcgggtgct cgacggtgcg atcgcgcttt
    12181 tcgattccgt ggccggggtc gagccgcagt ccgagacggt ctggcgacag gccgacaagt
    12241 acggggtgcc gcgcatagct ttcgtcaaca agatggaccg catcggcgcg gatttttaca
    12301 aggccgtagg caccatggtg gacaggcttg gggccaacgc cgttcccgtg cagttgccga
    12361 taggcgccga gagcgagttc caggggattg tggacctcgt ggagatgagg gccatcgtct
    12421 acaccgacga cctcggggcc acttgggagg tcaccgacat acccgaggac ataggtgctc
    12481 tggctgagga gtacagggag aagctcttgg aggcggtcgc cgatcaggac gaagagctca
    12541 tggtcatgta tctggagggc gaggagatcg aacccgacca gatccgggcc gccatccgtc
    12601 aggccaccct ggatattagg atgacacccg ttttcgtcgg ttccgcgttc aagaacaagg
    12661 gcgtgcagcc actcctcgac ggtgtagtcg actacctgcc gagcccgctc gatgttccgc
    12721 ctgtggaggg caggaccctc gacggagagg agatcacacg cgaggcggac gagaacggcc
    12781 cgctggcagc gttggccttc aaggtgcagg ccgatccgca cgtcgggaag ctgacctatg
    12841 tgcgggtgta ctcaggcacg ctcaaggccg gctcctacgt gatgaacacc accaagggca
    12901 ggcgcgagcg tgtggggcgt ctcttgcagc tgcacgccaa cagccgcgag cagcgagatg
    12961 aggtctatgc tggcgagctc gttgctgcca ttggcctctc cgatacgggg acgggcgaca
    13021 cgctcgtttc ggcggacgac gaggagcaga tcgtgctcga ggagatgatc ttccccgagc
    13081 ccgttatcag gcaggctatc gagcccaaga cgaaggccga ccaggagaag ctctccgttg
    13141 cgcttcagcg gctcgccgaa gaggacccga cgttcaccgt tcgcagcgac gaggagaccg
    13201 ggcagaccat cgtcgccggc atgggcgagc tgcacctgga gatcatcgtc gacaggctcc
    13261 tgcgcgagtt cagggttgac gcgaacatcg gccgtcctca ggttgcttac cgcgagacca
    13321 tccgcaagcg cgtggagggc atcgagggcc ggttcgtgcg gcagacgggt ggccggggcc
    13381 agtacggcca cgcggtcatc agcgccgagc acaacgacga ggcaggctac gagttcgaca
    13441 acaagatcgt cgggggcgtc atcccgaggg agtacatccc aagcgtcgat aagggcatca
    13501 gggaagcgct ggattccggc gttatcgcgg gctaccccgt ggtcgatgtc agggtcgagc
    13561 tggtcgacgg ttcctaccac gaggtcgact catccgagat ggcgttccag atcgctggtt
    13621 ccatggccat caaagaggcc ctcaagaggg ccaacccggt ccttctcgag cccgttatgg
    13681 acgtcgaggt agtggtgccc gaagagttca tgggcgacgt gatgggcgac ctctcggcta
    13741 ggcgtggcca tatacagggt atggactccc gcggcggagg acaggttatc cgcgccatgg
    13801 tgccgctctc cgagatgttc ggttacgcga cgaccgtgcg ctccaagacc cagggccgcg
    13861 ctacgttcac tatgcagttc gatcattacg ccgaggtgcc gaagagcata gcggccgaga
    13921 tagcagaaag gtaggagagg atgtcaagag gcaggttcga gcgcacaaag ccacacctca
    13981 acgtgggcac catcggtcac gtcgaccacg gcaagaccac cctaactgcg gccattacaa
    14041 gggtccttgc caaggaggtc cccga
//
LOCUS       DASTKM010000065        16715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_540977, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000065 DASTKM010000000
VERSION     DASTKM010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16715
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540977"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            856..1128
                     /locus_tag="VFQ10_02475"
     CDS             856..1128
                     /locus_tag="VFQ10_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964237.1"
                     /translation="MKPRVTAQLHSSSGRSSKILRDHTQIETVVEVAGVGRLVLDVDS
                     GGGYSLHAYPEDGEDPSRDLLAVGVVHADGIVAVHPGGLSAVDGRG"
     gene            1125..1526
                     /locus_tag="VFQ10_02480"
     CDS             1125..1526
                     /locus_tag="VFQ10_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964238.1"
                     /translation="MIEQISTAPVEVALLAAFTFILGAFALILWDLLRGRRPGWLRVM
                     AVIVGAPACALAATVISAGVGWAVAATLEPDEQPPARTEQTTPENRERTSPAEGRGPE
                     TTTERSASPTATATASPTVTASATATASPSP"
     gene            complement(1906..1995)
                     /locus_tag="VFQ10_02485"
     tRNA            complement(1906..1995)
                     /locus_tag="VFQ10_02485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1959..1961),aa:Ser,seq:gct)
     gene            complement(2057..3064)
                     /locus_tag="VFQ10_02490"
     CDS             complement(2057..3064)
                     /locus_tag="VFQ10_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9964239.1"
                     /translation="MASDAAETKLRKVARRPARGSLGNFLGDLRDLLRYETLKATVKE
                     FMRDDALGLGAQLAFYLILAIFPFILFCVAILDAFSSASPQFVTELFDYLRRLLPAQV
                     FGLIQTYTENTLRNEDTAPGLLSVGILGTIWAASGAFAALMNALNRAYDVQETRPFWK
                     VRGIAILMTLGLSALILIGVLLLVAGPSIGRTLAEVLTLGDEFMFVWNIVRWPAALLF
                     MVVTVALLFYFAPDANQPFRWITPGGVVGILLWVLASVAFNLYLSSDFNTYNKTYGSI
                     GTVIILLLYLYISSLTILFGATLNATLVRIKEEVSGEQILDAEPANDKTDVIEDEIKK
                     E"
     gene            3301..3858
                     /locus_tag="VFQ10_02495"
     CDS             3301..3858
                     /locus_tag="VFQ10_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9964240.1"
                     /translation="MNRVEDEVLVRRVASGDERALSGLYDRYAGLIYGTGMRYLGDRG
                     TAEELVQDVFTSVWKNAAGFDPARATFATWVYRITRNRATDLIRRRKARVRTIDGEPL
                     FEPGETDPSGELSQNFDVAALLSRLTPVHREVLILAYFHGLSQREISDRTATPLGTVK
                     SRTTAALRALRETMRFDEDRWSADE"
     gene            3851..4516
                     /locus_tag="VFQ10_02500"
     CDS             3851..4516
                     /locus_tag="VFQ10_02500"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET9964241.1"
                     /translation="MSEHPGTRDLLGPYVMGTLEPYEEREVEDHLQRCTSCRQEAQEL
                     RLVHERLADLAYSTEAPPEDLGARVVAGIPRREAGRRVPIWVAAVAAAFCVLAVLGVI
                     ITPDLFGARASANLSPTGRAPDAGGEVSIRGTGENMEMRLEAWGLPPCKSEQYYELWL
                     VEGHERVSAGSFTVGQSGRVDVRLNVPSFAGSYPKIGVTAEYDKDPRASEAKMLSGEL
                     HEL"
     gene            4766..6877
                     /locus_tag="VFQ10_02505"
     CDS             4766..6877
                     /locus_tag="VFQ10_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HET9964242.1"
                     /translation="MRLIVAEKPSVGRDIASTLGRHRKEKGSLAGEGWTVTWALGHLA
                     ELAPPDAYGEEYKKWRLESLPILPERFKVRVNHKTREQFNVVKGLMRSPSVTEVVNAC
                     DAGREGELIFAYLYGLSGCRKPVLRLWISSLTHEAIREGFDALREGTSMKPLEDAARS
                     RGEADWIVGMNATRAYSVRFGGRGNVLSVGRVQTPTLNLLVDREKEIQDFEPEKFWTV
                     HARFSRDGSTYDGVWFQKKQNRLPEREAADRIAEKVRGGTGTVRKAQNKTATEKPPLL
                     YDLTELQRNANARYGFTAERTLRAAQALYEERKLITYPRTSSRYLSKDMVGGLEKRVE
                     AAGALPDLSPFAQKLLRGQKLPVSKRIVDDTKVTDHHAIVPTNKKSSGGLPSDEAKVY
                     DLVARRFVAVFFPAARFENTTVVTEVKEETFLSRGRLVLEAGWRALYPDGVGGRKEKE
                     PPVLPPIEVGQEWAVAKVGVKEGETKPPPRFSESALLGAMETAGKLVEDEELRQQMKD
                     SGLGTPATRAATIERLIKVGYIEREKKILVPTEKGRSLIGLLGESPLSSPELTTRWEE
                     RLAKMERGVERRPDFIADIKGFTTKLVEEVRGMEGERLTAPSRSREPLGTCPKCGAPV
                     VETKKAYGCSAWKKTGCDFAIWKQVSGKRLSEGQAKQLLARGRTAQMKGFKSKAGKPY
                     AAALKLDSEHKVRLDFENSRT"
     gene            7072..7827
                     /locus_tag="VFQ10_02510"
     CDS             7072..7827
                     /locus_tag="VFQ10_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9964243.1"
                     /translation="MSVGVNGVPALKIEKLRKTYSNGFLALGGVSLEVEAGKFFGLLG
                     PNGAGKTTLINGVVSLARPDSGSVEVFGKDAFREFREARRMIGVSPQEVNLDKFLTVE
                     ETLLFHAGYYGVPRRKARERAEELLERFELAQRRGQRTNTLSGGMKRRVLFARALMHD
                     PKVLFLDEPTAGVDVELRYKLWGYIRELNRGGMTILLTTHYLEEAEALCEEIALINEG
                     NIAAQGSSEDLKERYEARNVEEVYMKVIGHDLH"
     gene            7814..8581
                     /locus_tag="VFQ10_02515"
     CDS             7814..8581
                     /locus_tag="VFQ10_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9964244.1"
                     /translation="MIFTDRTFQTLLSREVLRFTRVWMQTIVPQLLTSLLYLVVFGIA
                     LGGRIKQIEGIPYLDYILPGIALMSLITNSQMNSSWSVFDAKRERYIDEVLISPMSDL
                     QITLAYSLGGMLRGLVMGAGVFIVGVPFVGVQIKHPLMLVVIGLLAAFAFSSLGTMVG
                     ALATRIEHISLVTSVIIQPLAFLGGVFYSVGMLPGVLKVVTFFNPIFYTVDAARYVTL
                     GVSDLNPYPTLGMVCLIAAICFGGAWWAISRGPNLRY"
     gene            complement(8637..8981)
                     /locus_tag="VFQ10_02520"
     CDS             complement(8637..8981)
                     /locus_tag="VFQ10_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964245.1"
                     /translation="MASTSVSANGPLRIGVIILTIGTAVIHLYLGLQGFPLFILNGLG
                     YLALLAALTLPIPRISDYRSLTRWVLAGYTTLTILLWILVGARNSIGYADKIIEIVLV
                     ALLVLEASRSHR"
     gene            complement(8986..10206)
                     /locus_tag="VFQ10_02525"
     CDS             complement(8986..10206)
                     /locus_tag="VFQ10_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9964246.1"
                     /translation="MTSDAWRRRSPLVFLFVTVFVDMIGYGIVVPLLPFYAGLYVSGA
                     VLVGLLGSLYAAMQFAGGPFLGGLSDRHGRRPVLLLCLLGTSLAYLLLGLAQTLASLV
                     LAVVLAGAASGTLATAQAYIADSTVKEDRARGLGLIGAAFGLGLMAGPALGGLLSLYS
                     LSAPALFASALALTNCVFGYLTLPESHARHLRRKVPLLRLDPISQLARILKMRHVQAL
                     LAAVLLLNLALAGLVTNFPLFSHVRFGWGTTSNAFFFAFVGVCAVATQGFLIGRLQPR
                     FGESRLLVGGLALVSLNLVLVALVPTGVFLYPVVGLLALGMGLAIPSLTAIISNRTPP
                     EAQGRLMGGLQAILSLALIVGPAAAGLAFDHLGIPAPYLIGGTLTLLALIVSGTDLLP
                     GRRAEALQRARTSG"
     gene            complement(10203..11102)
                     /locus_tag="VFQ10_02530"
     CDS             complement(10203..11102)
                     /locus_tag="VFQ10_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET9964247.1"
                     /translation="MDSISQLPTATEELHASSGCDDHLERLTQLCVDDLISAFGLGEV
                     SHGRTVMESISRIPARRLARQILTYDRIVGESGLGTGGAWALKRLSRNTSIEGQENVP
                     RVGPLLLVSNHPGLADAVALFAATPRDDLRVIAADRPFLEALPNTSRYLLTVSETSAG
                     RSGIVRAAARHLRSGGSVLTFPGGRIEPDPAVLPGAVRALDRWSSSADLFARLIPELV
                     VVPVVVSGVISPSALRNPLIHLRRHRSDREWLAATLQVLVPALRNVDARVEFGRPIHT
                     EPGAVGDAVIEETRRLIENRETA"
     gene            complement(11240..11641)
                     /gene="folB"
                     /locus_tag="VFQ10_02535"
     CDS             complement(11240..11641)
                     /gene="folB"
                     /locus_tag="VFQ10_02535"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET9964248.1"
                     /translation="MSEDRILLADMYFHGRHGTLQAEHDLGQPFVVSIELYLDLRPAG
                     TSDDLTKTVDYSEVHRMARHIVEGPPVQLTETLAEMIAAATLDEHPAVEAVKVKVAKP
                     HVRLDDTVLAGSAVEILRRREAEPGSQARQS"
     gene            11755..12405
                     /gene="ribA"
                     /locus_tag="VFQ10_02540"
     CDS             11755..12405
                     /gene="ribA"
                     /locus_tag="VFQ10_02540"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET9964249.1"
                     /translation="MPSIRRVARARLPTSHGGFGMFVYEAADSKEHVALTTGFEPGTA
                     GEDEAILVRLHSECLTGDVFGSRRCDCGEQLEDSMRLLREQGRGVLLYLRQEGRGIGL
                     ANKISAYALQEEGFDTVQANLVLGLPEDARDYRVAAEMLLDLGVHRAALLTNNPAKIE
                     GLRSHGIEVVERLPVQVQPNPFNLRYLRTKKEKMGHLFPASILHGGLPNGPANGAS"
     gene            12420..13088
                     /locus_tag="VFQ10_02545"
     CDS             12420..13088
                     /locus_tag="VFQ10_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HET9964250.1"
                     /translation="MRPSVTVSYAQTLDGRVATTTGESKWISCPDSLRFAHEMRASHD
                     AVLVGAGTVCKDDPRLTVRHVPGTSPLRVVVDSTLRTPPGAAVLADGAARGTVLAVTD
                     RAPEERCEKAHSLGATVMKLPSGLDGRVDLAALLEELVASGIGSVMVEGGATVITGFF
                     RAGLVDRIAVCVAPKILGEGIEAIGDLGIRDLSRSLTLADAAVEPYGVDLILSGRIEY
                     PDRG"
     gene            13201..14118
                     /locus_tag="VFQ10_02550"
     CDS             13201..14118
                     /locus_tag="VFQ10_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HET9964251.1"
                     /translation="MSAGTEMLVYRGEVPQDLRLDLPTFAGSYGFPIKYGYAAAGRVL
                     DTGPGVENLSPGDPVFVHHPHQEVFVVPSRMPVRLPDDLDPILGVFSANLETALNIVH
                     DTPVRLGETALVFGQGVVGLLVALLLKLAGVGPVLVVDTLEERRRLALAAGADGAFAP
                     GGLNDRVMEITDGRGADVAVETSGSGAALQSAIDAVVAEGTVVVASWYGTKPVTLALG
                     GHFHRGRVRLRSSQVGRLDPALAPRWDRARRMDTVLGLLGRLSLRDLISHRIPFGEAP
                     EAYTLLDERPEETLQVIFTYEGLRGGQDV"
     gene            14111..14488
                     /locus_tag="VFQ10_02555"
     CDS             14111..14488
                     /locus_tag="VFQ10_02555"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET9964252.1"
                     /translation="MYEIYVAARFEAAHRLVGDFGPAARTHGHTYRIEVIVRGQHLGD
                     DGTLYDIGELGRAVEGLAGSMHYRDLNEVPGLAEVNTTAEAVASYCWDKLARPLRGRG
                     LDSLAVRIWESPDAYAAREDALA"
     gene            14454..15569
                     /locus_tag="VFQ10_02560"
     CDS             14454..15569
                     /locus_tag="VFQ10_02560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9964253.1"
                     /translation="MPTQPAKTRWPEGVFLRVAFITVGDTGRKTGGYLYNGRVISGLR
                     QRGFEIEEVVAGGASPEEQRTAALRLESAFDPLRFDTIVVDALARIAVSKHLDLWLSW
                     RPVVALVHELPSVAGGGSRHETVASERTYEEPLLRADRLVAVSDHGRKVLLGRGISPR
                     RIHVVPPGFDGVPVSGGSHVQSDGPVRALCVAQWIERKGILPLVEAWTLRERKGAVLE
                     LIGETDADPDYAVLVRAAIEAAPRGSIVVSGCVDEASLGASYASADLFVLPSRYEGYG
                     IVYAEALARGLPIIACDTGPVPDLVGREAAVLVQPDDREDLSAALDLLLGDPTLRMGM
                     SAAASRRASRLPRWEDTVAGFEQVLGTAAETRKSGPM"
     gene            15579..16451
                     /locus_tag="VFQ10_02565"
     CDS             15579..16451
                     /locus_tag="VFQ10_02565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9964254.1"
                     /translation="MDRRDHREQNRVAWNAVVGAHESHRGDLSRFISEGGNTLFQEER
                     DLLGELEGRSLVHFQCNSGGDSISLAKLGATVTGVDISDEAVSRARDLTKKTGTRATF
                     ERADVYDWLEEATREGRHYDIAFASYGVICWLPDLRGWARGTAGILNPGGRFVLVDFH
                     PAADIFDKDWNHVHDYPSGGEPLPLEEGVGDYVAVSGGGLTPAGFVEGVRDFQSPEGC
                     YLFRWGLGEVITALAEAGLRITALEEYPYANGERKFAGMRELRGKRMVPPEDVPTIPL
                     MYGIRAEKDQHPAT"
     gene            complement(16482..>16715)
                     /locus_tag="VFQ10_02570"
     CDS             complement(16482..>16715)
                     /locus_tag="VFQ10_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964255.1"
                     /translation="LGSSIQVALLVTPILVFLGPLLGHPLRLIFTPLELGALGAAVVV
                     TGFVALDGRSNWLEGAMLCGVYVIAALAFFFSP"
BASE COUNT         3263 a         4973 c         5556 g         2923 t
ORIGIN      
        1 ctccgaggcc catgcggagc aaatcatcgg gagcctcgaa gatcctggca acccctaagg
       61 tcctcgagac agggaagcat ccattcatcc aatgacttag aagtagaatt ccacgaagtt
      121 cgcgctagca aggagtcgcg cttcaaatag gagagggccc aagaaggttt ctctcataag
      181 aatagcgaag aatagcgtcg ggcagagggt agtagagaca caccttccgg cttggcacaa
      241 gaaaagtcgg tcagttacca gagattgcgc tttcatgaga aggaatgcgc gatatgttgc
      301 gtacaaaggt taccagctgt ttgaaacccg cttgctctcc ggcagcgttc gctgttataa
      361 gtaccgaaga gcacatggcg tccttgcaag ggagctatcc gagggaggca aagagtatga
      421 ccgaacgtga cgaccatcga acgggctccg ggggggtcaa ggacactgtc cgggacgagc
      481 tggccgagtc gcaagatcgt cgctagccgg aaatagccaa tccgcgaaac ctgggctggg
      541 gtcctaaggg gctccggccc ttctcctctt gtccctattc accggagtgc gtggaagggg
      601 aattctcaga agttgggcgg gcatctgaaa caaaatcgtc ttgacacgtc tgccacagtc
      661 gctactatgt tcccttggta aaggaacaac ccaaagagta gaggcttaga agcccgcccc
      721 aattatgggc gcttaagaga cgggcagagc cagtagcgca accgactacc gcattctcgg
      781 tagtcggttg cgtttcgctt tgggcgaaag gacgtgagag ggcttgagcc ttcgtaggct
      841 ccgggacgga cacgcatgaa acctagggtg acagctcagc ttcattcatc ttcgggcagg
      901 agctcgaaga tcctcagaga ccacacccaa atcgagaccg tcgtggaagt ggccggggtg
      961 ggtaggttgg tactagatgt ggacagcgga ggcggctact cgctgcacgc ttaccccgag
     1021 gatggcgaag atccgagccg agacctgctc gcagttggcg tggtgcatgc cgacgggatc
     1081 gtagccgtcc acccgggggg gttgtctgct gttgatggtc gaggatgata gaacagatca
     1141 gcaccgcgcc ggtggaggtg gcgctgttgg ccgcgttcac ctttattctg ggagcgttcg
     1201 ccttgatcct gtgggacctc ctccgagggc ggaggcccgg atggctgcgc gttatggcgg
     1261 ttatcgtggg ggcccctgcg tgcgccctgg ccgctacggt gatctcggcg ggggtcggat
     1321 gggcggtggc tgcgacgctt gagcccgacg agcaaccgcc ggcgcgcacc gagcagacga
     1381 caccagaaaa ccgagagagg acctcgcccg ccgaaggtag gggccccgag acgaccaccg
     1441 agcggagtgc gtcccccacc gccacggcta cggcttcacc cacggttacg gcttcagcca
     1501 cggctacggc ctcaccttca ccctagcaat agtaagagta gtcaggcaat acgatgaaca
     1561 tccatcacga ataacctatc acattgggag gctccgatcc ttctttttgc ccttattcac
     1621 ccaacctcgt agaagaggaa ttctcggaaa gtcacatcca ggattttgca tatgaggaaa
     1681 cattgagaca tcttttggtc aagcgaggcc gagatacgct gcgaaggcga ccacaacttc
     1741 tggtgccgtg gatacaaatc caagattacg aatgcggcgt tcgtggccgc ataaacgggt
     1801 gttctttgct gcgttggtgt attcgttggt gtagtaagag ccggaaggag ttccggccct
     1861 taccctgagc ttcccgttta tgctgcaaat agacgactct tgaagcggag gaggagggat
     1921 tcgaaccctc gataccccgt tgagaggtat aacaccttag cagggtgccg ccttcaacca
     1981 ctcggccact cctccgggtc acaggagtat atcaaagacg tgttgcgtct tcgtgcgctg
     2041 gcctctgagc cttctcctat tctttcttga tctcgtcctc gataacgtcg gtcttgtcgt
     2101 tggccggctc ggcgtcgagg atctgctcgc cggagacctc ctcttttatc ctgacgagcg
     2161 tggcgttgag ggttgcgccg aagaggatag tcagggagga gatgtagagg tagaggagca
     2221 ggatgatgac ggtgcctatg gagccgtagg tcttgttgta ggtattgaag tcgctactca
     2281 ggtacaggtt gaaggcgacg ctggcaagaa cccacagcag gatgcccacg acgccgccgg
     2341 gcgtgatcca gcggaacggc tggttggcgt caggggcgaa gtagaagagc agggctaccg
     2401 tcacgaccat gaacaagagc gccgcgggcc agcgcactat gttccagacg aacatgaact
     2461 cgtcgccgag agtaagtacc tcggcgagcg tcctaccgat ggacgggcct gctaccagga
     2521 gcagtactcc tatcagtatg agggccgaga ggcccagcgt cataaggatg gcgatgcccc
     2581 tgaccttcca gaacggccgc gtctcctgca cgtcgtaggc gcggttgagg gcgttcataa
     2641 gggccgcgaa ggctcctgaa gcggcccaga tggtaccaag gattccgacg gataggaggc
     2701 ctggcgccgt gtcttcgttg cggagcgtgt tctcggtgta ggtctggatc aggccgaaga
     2761 cctgcgccgg caggagccgc ctcaggtagt cgaagagttc cgtgacgaac tgcgggcttg
     2821 cgctggagaa ggcgtcgagg atcgcaacgc agaacagtat gaaagggaag atggcaagga
     2881 tcaggtagaa ggcgagctgc gcgccgagac cgagggcatc gtcgcgcatg aactctttta
     2941 cggtcgcctt gagcgtctcg tagcggagaa ggtccctcag gtcgccgagg aagttgccta
     3001 gcgacccccg cgcaggcctt cgggcgacct ttctgagctt tgtctcggcc gcgtctgatg
     3061 ccacctgggc atacctccct tctcgtaacc agccctattt taaccgcaat ccccatcgtg
     3121 gagggcgaag acgctaatcc ggtaagcccg gccggtcgta taaatctgtg gatggaaaag
     3181 atgttatcgg tgagggccga atgtctcgcg ccggatcacg cacgcggatg ggatatcatc
     3241 tgctccgtgt ggcgtgggag cggagagtcc gttcccgcag gcggttgggg aaggctctgg
     3301 atgaaccgtg tagaagacga ggttctagtg cgaagggtgg catccgggga cgaacgcgcc
     3361 ctctctggac tctacgaccg ctacgccggg ctgatctacg ggacggggat gcgttacctg
     3421 ggagacaggg ggacggctga ggaattggtt caggacgtct tcacctcagt gtggaagaac
     3481 gcggctggct tcgaccccgc gcgggcgacc ttcgccacgt gggtgtaccg catcacccgt
     3541 aaccgggcga cggacctgat ccgtcgccgc aaggcgcgtg tcaggaccat cgacggtgaa
     3601 ccgctcttcg agccaggaga gacggacccc tctggcgagc tctcacagaa cttcgacgtg
     3661 gccgccctgc tctcgcggct cacaccggtt cacagggagg tgctcatcct cgcttacttc
     3721 cacggcctct cgcagcgtga gatctccgac cgcaccgcta cgcccctggg tactgtaaag
     3781 agccgcacta cggcggctct gcgcgccctg cgcgagacaa tgaggttcga cgaggaccgg
     3841 tggtccgccg atgagtgagc accctggcac gagggacctc ctcggccctt acgtgatggg
     3901 taccctcgaa ccttacgagg agcgggaggt agaggatcac ctgcaacggt gcacgagttg
     3961 caggcaggag gcacaagaac tacggctggt ccacgaacgc ctcgctgatc ttgcgtacag
     4021 caccgaggcg ccaccggagg atctcggggc cagggtcgtg gcggggatac cgaggcgtga
     4081 agccggccga cgcgtcccga tctgggtggc cgctgtcgcc gcggccttct gcgtgctcgc
     4141 tgtgctcggc gtgatcatta cccctgatct cttcggggcc agggcctcag cgaacctgag
     4201 ccccaccggc cgcgcacccg atgcgggcgg cgaggtgagc atccggggca caggcgagaa
     4261 catggagatg cgcctcgaag cgtggggatt gccaccatgc aagagcgaac agtactatga
     4321 gctgtggctc gtcgagggcc atgaacgagt aagcgcaggg agctttacgg tcggacagag
     4381 cggccgggta gacgtaaggc tgaacgttcc gagcttcgcc ggttcctacc ccaaaatcgg
     4441 agtcactgcc gagtatgaca aagacccccg cgccagcgaa gcgaagatgc tctcaggcga
     4501 gttgcacgaa ctctgaaaac aacctaacgt gccttgctcc ctttctgcag caaggggagg
     4561 atgaaagaca tgcgggtgcc ctctcctggc tgactcgcag accacggggc atcgaagggc
     4621 tcggaaccgc gccgccatcc gcggggtaag taagcttccc agtccacgta accacggtgc
     4681 gtcaccatcc gagtaggtcg gcgaagttct gcattccaga caatctcgtc tggctatcgt
     4741 ccctcccgta cgtatactcc tttgtatgag gctaatcgtc gccgaaaagc cgtccgtcgg
     4801 cagggacata gcgagcaccc tggggaggca tcggaaggag aaaggatcac tggccggcga
     4861 aggctggacc gtgacctggg cgctcgggca tctggccgaa ttggcaccgc cggacgctta
     4921 cggcgaggag tacaagaagt ggcgtttgga gagcctgccg atcctgcctg aacgcttcaa
     4981 ggtcagggtg aaccacaaaa cccgcgagca gttcaacgtg gtaaagggtc tgatgagaag
     5041 tccctctgtg acggaggttg taaacgcctg cgacgcgggt cgggagggag agctcatctt
     5101 cgcctacctg tatggtctct cggggtgcag gaagcccgtg ctgcggctct ggatctcctc
     5161 cctcacccac gaggcgatac gggagggttt cgacgcttta cgtgagggca cttcgatgaa
     5221 acctctcgaa gacgcagccc gcagccgggg agaggctgac tggatcgtcg gcatgaacgc
     5281 cacccgcgcc tactcggtcc gcttcggagg ccgtggaaac gtactctctg tgggccgcgt
     5341 ccagaccccg accctgaacc ttctcgtcga ccgtgaaaag gagatacaag acttcgagcc
     5401 cgagaagttc tggaccgtcc acgcccgctt ctcgcgtgac gggagtactt acgacggcgt
     5461 ctggttccag aaaaagcaga accgcctgcc cgagagagag gccgcggacc ggatcgcgga
     5521 gaaagtgcga ggcgggacgg ggaccgtccg caaagcgcag aacaagacgg caactgagaa
     5581 gccgccgctc ctctacgatc tgaccgagct ccagcgcaac gccaacgccc gctacggttt
     5641 cacggccgaa cgtaccttgc gtgccgccca ggcgctctac gaggagagaa agctgatcac
     5701 ctacccgcgc acctccagcc gctacctctc caaggacatg gtgggcggcc tcgagaagcg
     5761 ggtcgaggcc gcgggggcgc tccccgacct gtcgcctttc gcgcagaagc tcctcagagg
     5821 acagaagctc ccggtaagca agcgcatcgt ggacgacacc aaggttaccg accatcacgc
     5881 catcgtcccg acgaacaaga agtcttcagg cgggctgccg tcggatgagg cgaaagtcta
     5941 cgacctggtg gccaggcgct ttgtggccgt gttcttcccc gctgcccgct tcgagaacac
     6001 caccgtcgtg accgaggtca aggaggagac gttcctttcc cgtgggcgcc tggtgctcga
     6061 ggcggggtgg cgcgcgctct accccgacgg ggtgggaggg cggaaggaga aagaaccacc
     6121 cgtgttgccg ccgatcgagg tggggcagga gtgggccgta gcgaaggtcg gcgtcaagga
     6181 gggcgagacc aagccgcccc cgcgcttctc ggagtccgcg ctgctcgggg cgatggagac
     6241 tgcggggaag ctcgtcgagg acgaggagct caggcagcag atgaaggact cgggcctcgg
     6301 gacgccggcc accagggccg ccaccataga gcggctcata aaggtaggct acatcgagcg
     6361 cgagaagaag atcctcgtcc ctacagagaa aggaaggtcg cttatcgggc tcctcgggga
     6421 gagtccactc tcatccccgg agctcacgac ccgctgggag gagcggctcg cgaagatgga
     6481 aagaggcgtg gagcgccgcc ctgacttcat tgccgacata aaaggcttca ccacaaagct
     6541 cgtcgaagag gtccgcggga tggaggggga gaggctgacg gctccctccc gcagccgcga
     6601 gccgctaggt acttgcccca agtgcggagc tcctgtcgtc gagacgaaga aagcctacgg
     6661 ctgctcggcg tggaagaaga ccgggtgcga cttcgcgatc tggaaacagg tctccggcaa
     6721 gcgcctcagc gagggccagg cgaagcagct cctcgcaagg gggcgcaccg cacagatgaa
     6781 aggtttcaag agcaaagcag gcaagcccta cgccgccgcg ctcaagctcg atagtgaaca
     6841 caaggtgcgg ctagatttcg agaacagccg tacctagatc ctcctcgcgt cagagagata
     6901 actgtttctt ctgagttgtg cgctacactc tcgcgggtat gaaaactatt gtcttcgggg
     6961 ccgggttggc cggcctcacc tgcgcaaaag tactcagaga acgcggagtg gaggtctccg
     7021 tcttggaggc gtcagacggt gtcggcgaga aggccgcggg gtggtgattg cgtgagcgtt
     7081 ggcgtaaacg gggttccggc actaaagata gagaagctca ggaagactta ttctaacggg
     7141 tttctggcac ttggtggggt ctcgctcgaa gtggaggcag gcaagttctt cgggttgcta
     7201 ggacctaacg gtgcaggaaa gacgacgctc ataaacggcg tggtcagcct ggccaggccc
     7261 gattcaggct cggtcgaagt cttcggaaag gacgccttca gggagtttcg ggaggcacgg
     7321 cgtatgatcg gggtctcccc ccaggaggtg aacctggaca agttcctgac cgtcgaggag
     7381 acattgcttt tccacgctgg atactacggc gtgcccagga ggaaggcccg tgagagagcc
     7441 gaggagcttc tggagcggtt cgaactcgcc cagaggcgtg ggcagaggac gaacacgctc
     7501 tcggggggga tgaagcggcg cgttctgttc gccagggcgc tcatgcacga cccgaaggtg
     7561 ctgtttctgg acgagccgac ggcgggcgta gacgtggagt tacgctacaa gctgtggggc
     7621 tacataagag agctaaaccg tggaggtatg accatcctgt tgacgacgca ttatctggag
     7681 gaggccgagg cgctgtgcga ggagatcgcg ctgataaacg agggtaacat cgcggcccag
     7741 gggagcagcg aagacctcaa ggagcgctac gaggcaagga acgtcgagga agtctacatg
     7801 aaggtgatag gccatgatct tcactgatag gactttccag accctcctct cccgcgaggt
     7861 gctgcgattc acgcgggtct ggatgcagac catagttccg cagcttttga cctcgctcct
     7921 ctacctggtg gtcttcggga tcgcgctcgg cggccgcata aagcagatag agggcattcc
     7981 ctacctcgac tacatcctgc ctggcatagc cctgatgagc cttataacga actcgcagat
     8041 gaactcatcc tggtcggtct tcgacgccaa gcgcgagcgc tacatagacg aggtgctcat
     8101 cagcccgatg agcgacctgc agatcacgct cgcctactct ttaggcggta tgctgcgcgg
     8161 gctggtgatg ggggcagggg tcttcatcgt cggggtgcct ttcgtcggag tccagatcaa
     8221 acacccgctg atgctcgtgg tgatcggact tctcgccgcc ttcgcgttct cctcgcttgg
     8281 cacgatggtc ggcgccctgg cgactaggat agagcacatc tcgctcgtca cgagcgtcat
     8341 catccagccc ctcgcgttcc tcggcggggt attctattcc gtcgggatgc tgcccggcgt
     8401 gctcaaggtg gtgacgttct tcaacccgat cttctacact gtcgacgcag cccgctatgt
     8461 gacgctcggc gtctccgacc tgaaccccta tccgaccctg ggtatggtct gcctcatcgc
     8521 cgcaatctgc tttgggggag cctggtgggc cataagccgt ggcccgaatc tgcgatactg
     8581 atgaggtgcg gcgctccacc agcgttgtcc atctccctcg agaatctcga aaggcatcaa
     8641 cgatgcgacc tgctggcttc tagcacgaga agtgctacca ggacgatctc gatgatcttg
     8701 tcggcgtacc cgatagagtt ccgcgccccg accagaatcc agaggaggat cgtcagcgtc
     8761 gtgtatccgg cgaggaccca ccgtgtgagg ctcctgtagt ccgagatcct tggtatcggc
     8821 aaagtcaggg ccgcaaggag cgcaagatac cccagcccgt tgaggataaa gagcgggaag
     8881 ccctgcaggc cgaggtagag gtggatcacg gctgtcccga tagtcaggat gatcaccccg
     8941 atcctgagcg ggccattcgc ggagacgctc gtagaagcca tgctcttacc ccgacgtcct
     9001 agccctttgt agagcctcgg ccctccttcc cggaagcaga tccgtccccg agacgatcag
     9061 ggcgaggagg gtcagggtcc cgccgatgag gtaaggcgca gggataccga gatggtcgaa
     9121 ggcgagcccg gcggccgcgg ggcccacgat cagggccagg ctgaggatgg cctgcagacc
     9181 tcccatgagc cttccctgcg cctcgggcgg ggtccggttg gagatgatgg ctgtgaggct
     9241 agggatggcg agccccatcc ccagcgccag gaggccgacg accgggtaga ggaatacacc
     9301 ggtagggacc agtgctacga ggaccaggtt gagggagaca agggcgagtc cgccaacgag
     9361 gagtctgctc tccccaaagc gtggctggag gcgtccgatc aggaatcctt gcgtggcgac
     9421 ggcgcacacc cctacgaaag cgaagaagaa ggcgtttgaa gttgtccccc acccgaaccg
     9481 gacgtggctg aacagcggga agttagtcac gagcccggcg agagccaggt tgagcagtag
     9541 cactgccgcg agcaaagcct gaacgtgtct catcttcagt atgcgggcca gttgcgagat
     9601 ggggtcgaga cgcaagaggg ggaccttcct gcgcaggtgg cgcgcatgcg attcggggag
     9661 ggtcaggtag ccgaagacgc agttggtaag ggcaagggct gaggcgaaga gggctggtgc
     9721 gctcaatgag tagaggctga ggaggccgcc gagggctggt cctgccatga ggccgagccc
     9781 gaaggcagcc ccgatcaggc ctagcccccg cgccctgtcc tccttaaccg tgctgtcggc
     9841 tatgtaagcc tgggcggtcg ccagcgtgcc gctcgcggcg cctgccagga cgacagcgag
     9901 gacgagtgag gcgagcgtct gggcgaggcc gagcaaaagg taggcgagcg aagtcccgag
     9961 caggcaaagc aggaggacag ggcgtctccc gtggcggtcc gagagcccgc cgaggaaggg
    10021 accgccggcg aactgcatcg cggcgtagag ggagccgagc aggccgacaa gtactgcgcc
    10081 agaaacgtac aggccagcgt agaagggtag caaggggacc acgatgccgt acccgatcat
    10141 gtccacgaac accgtgacga acaggaacac tagcggcgat cggcgacgcc acgcgtccga
    10201 cgtcacgccg tctccctgtt ctcaatcagc cgacgcgtct cctcgatcac cgcgtcgccg
    10261 acggctccgg gttcggtatg gatgggacgt ccgaactcga ccctggcatc cacattgcgc
    10321 agggccggga cgagcacctg gagcgtggcg gcgagccact cgcggtcgct cctgtggcgg
    10381 cgcaggtgga tcagggggtt ccggagggcc gaaggtgaga tcacaccgct caccacgacg
    10441 ggaacgacga caagctccgg tatgaggcgg gcgaagaggt cggcgctcga ggaccagcga
    10501 tccagtgctc tgacggcgcc gggcaagacc gccgggtcgg gctcgatcct gccccctgga
    10561 aacgtgagta cgctgccgcc gctacgcagg tgcctggccg ccgcccgcac gatccccgaa
    10621 cgtcctgcgg aggtctccga gactgtcaga aggtaacgcg aagtattggg gagggcttcg
    10681 agaaagggtc ggtcggcggc gatgacacgc agatcgtccc tcggcgtcgc cgcgaagagg
    10741 gcgacggcgt ctgcgaggcc tggatggttg gagacgagga gcagcggccc cacgcgcggt
    10801 acgttttcct gaccctcgat gctggtgttg cgggaaagcc ttttcagcgc ccacgctccc
    10861 cccgttccca ggccagactc gccgactatt ctgtcgtaag tcaggatctg gcgcgccaga
    10921 cgccgggctg gtattcgcga gatggactcc attaccgtac gcccgtggct tacctcgccc
    10981 aggccgaagg cggagataag gtcgtccaca caaagctgcg tgagacgttc gagatgatcg
    11041 tcgcaccccg aagaagcgtg gagctcctcg gttgcggtcg gcagttggga gatgctatcc
    11101 atggcgcttc cccgtcagcc ttgtggtcac cacgtgtgcc accctttcgc ttgcctcaaa
    11161 cgatgatcgg ggcgcctcct cgttatctgt tagtacgcac cggccgcagg tgcggttcgg
    11221 gtaccgttat gaggataccc tatgactggc gtgcctggct tcccggttcg gcttcgcggc
    11281 ggcgtaggat ctcgacggcc gagccggcga gtacggtgtc gtcgagcctg acgtgaggct
    11341 tggcgacctt cacctttacg gcttcgaccg ccggatgctc gtcgagcgta gctgcagcga
    11401 tcatctctgc cagggtctcg gtaagttgca cgggcggtcc ctcgacgatg tggcgcgcca
    11461 tacggtggac ctcactgtag tccacggtct tcgtcaggtc gtcggaggtc cccgcgggtc
    11521 tcaggtcgag gtacagttcg atgctcacga cgaaaggctg gccaagatcg tgctcggcct
    11581 gcagggttcc gtggcggccg tgaaagtaca tgtccgcaag aagtatgcga tcctcgctca
    11641 tcgactcctt tcgctccctt gcatccgaat cagtatgcca gggattagac tgagagggcg
    11701 gctgtagtag aatctcggtc tactcgacca cgacctagat cggacacagg gaccatgcct
    11761 tcgatccgac gtgttgcacg agccaggtta ccaacttccc acggcggctt cgggatgttc
    11821 gtctacgagg cggcagacag caaggaacac gtggcgctca ccaccggttt cgagcctggg
    11881 actgctggcg aggatgaggc catcctggtt cgactgcact ccgagtgcct gacgggagac
    11941 gtcttcggct cgcgacgttg cgactgcggg gagcagctcg aggatagcat gaggctcctg
    12001 cgcgagcagg ggcgcggtgt cctcctctac ctgaggcaag agggacgcgg catcgggctc
    12061 gcgaacaaga tctccgccta cgcgctccaa gaggagggat tcgacaccgt ccaggcgaac
    12121 ctggtccttg gcctacccga ggacgcgcgc gattatcggg tcgccgccga gatgctcctg
    12181 gacctcggcg tgcaccgggc ggccttgctg acgaacaacc cggcgaagat agaaggcctc
    12241 agaagccacg gcatcgaggt cgtggagcgc ctgccggtcc aggtccaacc caacccgttc
    12301 aacctcaggt acctgcggac gaagaaggag aagatgggac acctcttccc ggcttcgatc
    12361 ttgcacggag ggctgcccaa cggcccggcg aacggagcat cgtgatggga gaggcctcta
    12421 tgcggccttc cgtcaccgtg agctacgcgc agacgctcga cgggcgcgtg gcgaccacga
    12481 cgggggaatc aaagtggatc agctgccctg actccctgcg cttcgcccac gaaatgcgcg
    12541 cctctcacga cgccgtcctc gtcggcgccg gaacggtctg caaggatgat ccccgtctga
    12601 cggtccgtca cgtccctggc accagcccgc ttcgcgtggt cgttgacagc accctgcgca
    12661 ccccccccgg cgccgccgtg ctcgctgacg gcgcggcccg tggaacggtg ctcgccgtta
    12721 cggaccgcgc gccggaagaa cgctgcgaga aggcccactc tctgggcgcc accgtcatga
    12781 agctgccttc tggtctcgac ggacgtgtcg acctcgcagc actcctggag gagttggtag
    12841 cgtcaggcat cgggtcggtg atggtcgagg gaggggccac cgtgatcacg ggctttttca
    12901 gggccgggct ggtcgaccgg attgccgtgt gcgtcgcgcc caagatattg ggtgaaggca
    12961 tagaagccat cggcgacctc ggcatccgcg acctctcccg gtctctcacc ctcgccgacg
    13021 ccgcggtgga accttacggg gtggatctta tcctcagcgg acgcatcgaa tacccggatc
    13081 ggggatgaac tgaaggccag agccctctgg tttaccgccc cacgcgcggc cacgctccgc
    13141 gcggagacgg tagagcctcc cggacccggc gatgtacgtg tcgagaccat cgcctccgcc
    13201 gtgagcgcgg gcacagagat gttggtctac cgcggcgagg tcccgcagga tctccgattg
    13261 gacctgccca ccttcgcagg gagctacggg ttcccgatca aatacggcta cgccgccgca
    13321 gggcgtgtcc tcgacaccgg tcctggcgtc gaaaacctct ctccgggaga tcccgtcttc
    13381 gttcaccatc cgcaccagga ggtcttcgtc gtcccctcgc ggatgcccgt gcgactcccc
    13441 gacgacctcg accccatact cggcgtgttc agcgccaacc tcgagacggc cctgaacatc
    13501 gtacacgata cgcccgtgcg actgggtgag acggccctgg tcttcggcca gggcgtcgtc
    13561 ggtttgctcg tggccctgct cctgaagctt gccggcgttg ggcctgtact cgtggtcgac
    13621 actctcgagg agcgtcgcag gctggcgctc gccgcgggcg ctgacggcgc gtttgcgcca
    13681 ggagggttga acgatcgtgt catggaaatc acggacgggc ggggggctga cgttgcggtc
    13741 gagaccagcg ggtccggggc cgctttgcag agcgcgatag acgctgtggt ggccgagggg
    13801 acggtcgtcg tggcttcgtg gtacggcacc aagcccgtca cgctcgcgct cggaggacat
    13861 ttccataggg gaagggtgag gctcagatcc tcgcaggtcg gtcgcttgga ccctgcgctc
    13921 gcgccgcgct gggacagggc ccggcgtatg gacacggttc tcggcctgct gggccggctg
    13981 agcctgaggg acctcatctc ccaccgtatc ccgttcggag aggcgcccga ggcttacacg
    14041 ctcctcgacg agagaccaga agagacgctg caggtgatct ttacctacga gggactccga
    14101 ggaggccagg atgtatgaga tctacgttgc cgcgaggttc gaggctgcgc acaggctcgt
    14161 cggagacttc gggcccgcgg ccaggacgca cggacatacc tacaggatag aggtgatcgt
    14221 gcgcggccag catctcggag acgacggcac gctctacgat atcggtgaac tggggcgggc
    14281 tgtagaggga ctggccggct ccatgcacta cagggacctg aacgaggtcc cagggctggc
    14341 ggaagtcaat acgacggcag aagcggtggc ctcctactgt tgggataagc tggcacgacc
    14401 cctccgcggc cgggggctcg attcgctcgc ggtgcgcatc tgggagtccc ccgatgccta
    14461 cgcagcccgc gaagacgcgt tggcctgagg gggtcttcct gcgcgtcgct ttcatcacgg
    14521 tgggagacac gggccgcaag accggcggct acctgtacaa cgggcgtgtg atctccggcc
    14581 tgcgccagag ggggttcgag atcgaagagg tcgtggccgg tggagcatcg cctgaggagc
    14641 aacgaacagc cgccctacga ttagaatccg cgttcgaccc tttgaggttc gacaccatag
    14701 tagtcgacgc cctcgcccgc atcgcagtgt ccaagcacct ggatctctgg ctgtcctggc
    14761 ggcctgtagt agcgcttgtc cacgaactgc cgagtgtcgc aggcggcggg tccaggcatg
    14821 agaccgtagc cagcgagcgg acctacgagg agccgctgct gcgcgcggac aggctcgtcg
    14881 ccgtaagcga ccatggaagg aaagtgctct taggacgggg tatctctccc cgacgtatcc
    14941 acgtcgtgcc gccgggcttc gacggcgttc cggtcagcgg tggatctcac gttcagagcg
    15001 acggtcccgt gcgcgccctc tgcgtcgcgc agtggataga gcgcaagggg atactgcccc
    15061 ttgtcgaagc gtggacgctg cgtgagcgta agggcgctgt gctagagctg atcggggaga
    15121 cagacgctga ccccgattac gcggtcctgg tcagggcggc catcgaggcc gcaccccggg
    15181 gctccatcgt cgtatccgga tgcgtggacg aggcgtcgct cggtgcctct tacgcgtcgg
    15241 ccgacctctt cgtcttgccc tcgcgctacg agggatacgg gatcgtctac gcggaagcac
    15301 tcgcccgcgg gctcccgata atcgcctgtg acaccggtcc agttcccgac ctggtcggtc
    15361 gggaggccgc ggtattggtt cagccggacg acagggagga tctctcggcg gccctggatc
    15421 tcctgctcgg ggatcccacg ctcaggatgg ggatgtccgc cgccgcaagc cgccgggcct
    15481 cccgcctgcc gcgttgggag gatactgtcg ccggcttcga gcaggttctc gggaccgcgg
    15541 ccgaaaccag gaagtcgggg ccgatgtaga atctccccgt ggaccgtagg gaccatcgag
    15601 aacagaaccg ggtggcgtgg aacgccgttg tcggtgccca tgaaagtcac cgcggggacc
    15661 tctcacgctt cattagtgag ggcggcaata cgctcttcca ggaggagcgt gatcttctcg
    15721 gtgaactcga aggaagatcc ctggtccact tccagtgcaa ctcagggggc gactcgataa
    15781 gcctggcgaa gctaggcgcg acggttacgg gtgtggatat tagcgacgaa gccgtctccc
    15841 gtgcccggga tctcacgaag aaaaccggca caagggccac gttcgagcgc gccgacgtgt
    15901 acgactggct cgaggaagcg acacgcgagg gcaggcacta cgacatcgcc ttcgcctcct
    15961 acggtgtcat ctgctggctg ccagatctca gaggctgggc ccggggcacc gccggcatcc
    16021 tgaaccccgg cgggcgcttt gtgctggtgg atttccatcc tgcggccgac atcttcgaca
    16081 aagactggaa ccacgttcac gactatccct ccggcgggga accgctaccc ctggaagaag
    16141 gcgtcggcga ctacgtggcc gtgtcaggcg gaggtctcac cccagccggg ttcgtcgagg
    16201 gggttcgaga cttccagagc cccgaaggct gctatctctt tcggtgggga ctcggggagg
    16261 ttattacggc gctggccgaa gcgggactca ggatcacggc tctcgaagaa tacccgtacg
    16321 cgaacgggga acggaagttc gccgggatgc gcgagctacg ggggaaacga atggttccgc
    16381 ctgaagacgt cccaaccatc cccttgatgt acggcatccg tgcggagaaa gatcagcatc
    16441 cggctacata accgaaacaa accagagtgc ctgaaacctc tctatggcga gaagaagaac
    16501 gcgagggcgg cgataacgta gacgccgcag agcatcgcgc cttctaacca attggacctg
    16561 ccgtcgaggg cgacgaagcc tgttacgacc acggcggccc cgagggcgcc gagctccagg
    16621 ggggtgaaga tcaggcgcag gggatggccg agcaggggcc cgaggaagac gaggatgggg
    16681 gtgacgagca gggcgacctg gatagaggag cccag
//
LOCUS       DASTKM010000066         6344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_364974, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000066 DASTKM010000000
VERSION     DASTKM010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364974"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="VFQ10_02575"
     CDS             <1..1440
                     /locus_tag="VFQ10_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET9964256.1"
                     /translation="VPLHERPDGRQLLEDAREGRFTAVLVSKLDRLGRSLLVIVDAHD
                     RLDAARATLRSGREPIDTSNPSGRLIFQMLASFAEYDRENIRERTQAGLHRAFRRGKH
                     MGRIPYGYRANADAQLVVEEQEAEIVREIIANIAGGSTLYAEAKRLNDSGVPAPGTRY
                     GTGERKPGRLWQSKTLSNIVHQSAYTGLHRVKINGGEEVIEREVPIILENTSVRDKAV
                     AALSENRRYRNRKTDRRYLLAGLVKCAHCGYGCSGHATWSKGKRYSYYVCIAKWPEKT
                     PKASPHKPPYVKAEWLEKTVWEDVRSFLENPGEVLERVREQMGDEDEAEKLERRRGDL
                     GKRLAGKQAEKDRYVRLYAQGHISEDELSVYLADLKNQIGNLRLLLESVEADLSQYAE
                     KREIAQTTSAWLATLQERISEVEAETEEAFQARRQLVKLLVAGVTIGRREDGEREIRI
                     TYRFGPPTEPNAEFIAEGMFVSSMENALS"
     gene            complement(1538..2053)
                     /locus_tag="VFQ10_02580"
     CDS             complement(1538..2053)
                     /locus_tag="VFQ10_02580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9964257.1"
                     /translation="MKGGVVGLRDLVVRCCEPEDYKAVHLVYSSPRAMAETLGVPYSS
                     EDAWREELARERDDNFSIVACVQGKVVGHLALSVYMNPRTRHSGHFGIAVRDDWQGKG
                     VGTRLMEACVDLADNWLGLTRLDLRVYTDNAPAIALYEKFGFKVEGTHRRFALRNGEY
                     VDAHVMARLLV"
     gene            2176..2430
                     /locus_tag="VFQ10_02585"
     CDS             2176..2430
                     /locus_tag="VFQ10_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964258.1"
                     /translation="MDQRNLGREGLVVSELGLGCMGMSEFYGTGDEEEFERREYLEEN
                     ASAASVTLTEDDLASIEGAMPRGSAAGERYAEEQMRAVGR"
     gene            2440..3039
                     /locus_tag="VFQ10_02590"
     CDS             2440..3039
                     /locus_tag="VFQ10_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565683.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HET9964259.1"
                     /translation="MTDVEHISRKGTGVPLSWFPIPEESELPEDLQGLFRKAREMVGF
                     VPNVFKAYSYRPERLSAWFAHYRQLHEPTPNLDAAEREMIAVAVSMANGCLYCLVAHG
                     AALRQALGDPILADRITLDHRRAGLDERRTAILDFAVKVTERPLDCDPGDLERLKGYG
                     LTEEEVWDVVEIASMYNFTNRMSLACGMIPNEEYHALAR"
     gene            3061..3315
                     /locus_tag="VFQ10_02595"
     CDS             3061..3315
                     /locus_tag="VFQ10_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964260.1"
                     /translation="MLQVISVLGALAILAAFAANLFGWIQPSNLWYSVANFLGSAVLT
                     VVAVVDQQLGFLLLEGTWALVSLWGIFAALRGRRAPPGAH"
     gene            3363..3563
                     /locus_tag="VFQ10_02600"
     CDS             3363..3563
                     /locus_tag="VFQ10_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964261.1"
                     /translation="MSGVKYVSEVKVEPVEGKIRRAYLPVQEDPVLFGVHSEVAEHYG
                     VSPENVEPYDTTLDYVVAAAGG"
     gene            3564..3827
                     /locus_tag="VFQ10_02605"
     CDS             3564..3827
                     /locus_tag="VFQ10_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9964262.1"
                     /translation="MLGTFSGALAARQVSFENLHAETVGEIEVESKVLVIKRIKQIFH
                     ITAQGRDRETIERALQVYADSCPVAASVKGSIEISSELDLTVA"
     gene            complement(3841..5313)
                     /locus_tag="VFQ10_02610"
     CDS             complement(3841..5313)
                     /locus_tag="VFQ10_02610"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9964263.1"
                     /translation="MTEMKATPEESIARVTVSADTGFLPPLIDFVKQITHRLGLGDAE
                     QVDHAVELVCTNIIEHAFGPEEEGSFDVQVLRRPGLVVVAVEDRGLPFDYKRLQDGED
                     RTVLEILHRSFDETRFINLGRRGNRVELLKHLPHTDVRDHLPEEEHRGTVEAAAAHED
                     VPLEIRMMRPDESFELSRCVYRSYGYSYDWDYIYYPDRIRELQEKGLMISCVAVTPEG
                     EFVGHLAITVERPGSLVGESGQAVVDPRFRGHHLFPRMKTFMAEWAADAGMYGLYSEA
                     TAIHPYSQRGNLHLGARETGFLLGYIPASVSYKQIGEEREGRRGSVALFYMRITDEPE
                     REIYPPPPYLEAVRRVVEHNGLRRTIGGVPDPTLQHSLMSVEVRQDHNLAFLRIEEPG
                     ADLEELVHRHLRDLSLHRVDCVYVDLPLSHPATQRSAAGLNNLGVFFGGIIPEAHDGG
                     GDVLRLQYLNNVEIEAGDVSTASDFGEELLGLIFRQNTLP"
     gene            5800..6021
                     /locus_tag="VFQ10_02615"
     CDS             5800..6021
                     /locus_tag="VFQ10_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964264.1"
                     /translation="MFNDWHYVLAQDREAREQDFAERVAERGRYARSPGRPSLRARVA
                     RRLFALAVAAESEETWRVVWERLEARGRL"
     gene            6063..>6344
                     /locus_tag="VFQ10_02620"
     CDS             6063..>6344
                     /locus_tag="VFQ10_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9964265.1"
                     /translation="MKALELRGVEKSFKKRRGEVVRALDSISLSVGKGEVVGILGPNG
                     SGKSTLVRVISTLVLPDSGEVEVFGIDATRHPKRVQRSMNRVSVEASFFK"
BASE COUNT         1299 a         1855 c         2023 g         1167 t
ORIGIN      
        1 gttccgctgc acgaaaggcc cgacggcagg cagctcttgg aagatgctcg ggagggcaga
       61 ttcacggcag tgctcgtctc caagctagac aggctgggac gttcactgct tgtcatcgtc
      121 gacgctcatg accgcctcga cgcggccagg gcaaccttgc gttccggccg cgaacctata
      181 gatacatcca acccttccgg aaggctgatc ttccagatgc tcgccagctt cgcggagtat
      241 gatcgcgaga atatccgaga gaggactcag gccgggctgc accgagcttt tcggagaggc
      301 aaacacatgg gacgcatacc ctacgggtac cgtgccaacg cggatgccca gcttgtggtc
      361 gaagagcagg aagcggagat tgtacgggag atcatcgcca acatcgcggg cggctcaaca
      421 ctttatgcgg aagcaaagcg cctgaacgat tcgggcgtac ccgctcccgg aacacgctac
      481 ggtaccggcg agcgcaagcc ggggaggctg tggcagtcta agaccctctc aaacatcgtc
      541 caccagagcg catacactgg tcttcacagg gtaaaaatta acggcggcga ggaagtcatc
      601 gagcgcgagg tgccgatcat cctggagaat acgagcgtta gggacaaggc cgtggccgcg
      661 ctttccgaga acaggcgcta tcgtaaccgc aagaccgacc gaagatatct gctggccgga
      721 ctggtgaagt gcgcacactg cggatatggg tgctctggac atgccacgtg gagcaagggc
      781 aaacgctact cctactacgt gtgtatcgca aagtggcctg agaagacccc taaagcgtcg
      841 ccgcacaagc cgccttacgt caaagctgag tggctggaga agacggtgtg ggaggacgtg
      901 cgatccttcc tagagaaccc tggagaggta ctggagcggg tcagagagca gatgggggac
      961 gaagacgagg cggagaagct cgaaaggcgt cggggagacc tgggaaagcg cttggccgga
     1021 aagcaggccg agaaggatcg ctacgtgcgg ctctacgcgc agggacacat ctctgaggat
     1081 gagttgagtg tgtacctcgc cgacctcaaa aaccagatcg gtaacctccg gctcctcctg
     1141 gagtcggtcg aggctgacct gtcacagtat gccgagaagc gggagatcgc ccagacgacg
     1201 agcgcgtggc tcgccactct acaggagcgc atctcagagg tcgaggcgga gactgaggaa
     1261 gccttccagg ctcgccgcca gcttgtgaag ctcctcgtgg ctggcgtcac gatcggtagg
     1321 cgcgaagacg gggaacgcga gatcaggatc acctatcgct tcgggccgcc aaccgagccc
     1381 aatgcggagt tcatcgctga gggtatgttt gttagcagca tggagaacgc cttgtcgtag
     1441 tcgttgccgc agacatcgca ctgggccatg gctcggctcc tttcctacgt ttacttcctt
     1501 tagatcatgc ccggtgtcag ggaatcgcaa tctgccccta caccagcaga cgggccatga
     1561 cgtgagcgtc cacgtactcg ccgttgcgga gtgcaaatcg cctgtgggtt ccctcgacct
     1621 tgaatccgaa cttctcgtag agtgcaatcg cgggcgcatt atcggtgtat acgcggagat
     1681 ccaacctcgt aaggcccaac cagttgtccg cgaggtccac gcaagcttcc atcaaccggg
     1741 tgccgacgcc cttgccctgc caatcatccc ttaccgctat accgaagtgt cctgagtgtc
     1801 gagtcctcgg gttcatgtag acactgagcg cgagatggcc gacgacctta ccctgtacgc
     1861 aggccactat agagaagtta tcgtctcgtt ctctggcaag ctcttcgcgc catgcatcct
     1921 ccgaagagta cggaactcca agtgtttcgg ccatagcccg tggggaggag tagacgaggt
     1981 gcacggcttt gtaatcttcc ggttcacagc accggacgac caggtcacgc aagccgacga
     2041 ctcctccttt cacacgagca tctcgagacc ccctattctc acacgcgaag ctgaaatgct
     2101 gaaatatgct cgcggcgaat tgtgtaataa actcgggcgg tggtatcaag aaacaccagg
     2161 ctagaggagt gatctgtgga tcagcgaaat ctgggtcgcg aagggctcgt cgtatcggag
     2221 cttgggctcg ggtgcatggg gatgagcgag ttctacggca cgggggacga ggaggagttc
     2281 gagcgccggg agtacctaga agaaaacgcc agcgccgcaa gcgtaacgct caccgaggac
     2341 gacctcgcca gcatcgaggg ggccatgcct cgcggatcgg ccgccggcga gcgctacgcc
     2401 gaagagcaga tgcgggccgt aggccgctag gaggagccga tgaccgatgt cgagcacatc
     2461 tcgcgcaagg ggactggtgt gcccctaagc tggttcccca tccccgaaga gtccgagctt
     2521 cccgaggacc tgcaggggct tttcaggaag gcgcgggaga tggtcgggtt cgttcccaac
     2581 gtcttcaaag cttacagtta ccgtcccgag cggctctccg cctggttcgc ccactaccgc
     2641 cagctccacg agccgacccc gaacctggac gcggcggaga gggagatgat cgccgtggcc
     2701 gtctcgatgg cgaacggctg cctctactgc ctggtcgccc acggcgccgc ccttcgccag
     2761 gccctgggcg accccatcct tgccgaccgc atcacgctag atcacaggag ggccggcctc
     2821 gacgaacgcc gcacggccat cctggacttc gccgtcaagg ttacagagcg cccgctggat
     2881 tgcgatccgg gagacctgga acgcctcaag ggctacgggc tgaccgagga ggaggtctgg
     2941 gatgtagtgg agatcgccag tatgtataac ttcaccaacc gtatgtcgct ggcgtgcggc
     3001 atgattccca acgaggagta ccacgcgctg gcaagatagt atttccgaag gtacccttct
     3061 gtgctgcagg tgatctcggt tctcggagcg ctggctatcc tcgccgcctt cgccgccaac
     3121 ttgtttggct ggatccaacc ctccaacctc tggtactcgg tagccaattt cctgggctcg
     3181 gccgtgctga cggtggtggc cgtcgtagac cagcaactgg gcttcctact cctggagggc
     3241 acctgggccc tggtgagcct ctggggcatc ttcgctgccc tgcgcgggag acgagcgccg
     3301 cccggggcgc actagagggg gacctgtata ctgcgggggc acacagcgga gaggagatga
     3361 gcgtgtcagg agtaaagtac gtctccgagg taaaagtgga gccggtggag gggaagatcc
     3421 gccgcgctta tctccccgtc caggaggatc cggtgctgtt cggtgtccac agcgaggtcg
     3481 ccgaacacta cggcgtctcc ccggagaacg tagaacccta cgacaccacg ctcgactacg
     3541 tggtcgccgc cgcgggcggc tgactgctcg gcactttttc aggcgcgctg gcagcgcgtc
     3601 aggtgtcgtt cgagaacctc cacgccgaga cggtcggaga gatcgaggtc gagagcaagg
     3661 tgcttgtgat caagaggatc aaacagatct tccacatcac ggcgcaaggg cgggaccgcg
     3721 agaccataga aagggcactc caagtctacg ccgatagctg tccggtggcg gcttccgtaa
     3781 aggggagcat cgagatctcc agcgagttgg acctcacggt cgcctgaacc gcctgcgagc
     3841 ctacggaagt gtgttctgcc tgaagatcag gccaaggagc tcttctccga agtccgatgc
     3901 tgtgctgacg tcgccggctt cgatctcgac gttgttcagg tactggagac ggagcacgtc
     3961 tcctcctccg tcatgggctt cggggatgat cccaccgaag aacaccccga ggttattcaa
     4021 cccggccgca gatctctgcg tcgcaggatg ggagagagga aggtccacgt agacgcaatc
     4081 cacgcggtgc aggctgagat ctcgcaggtg gcggtggaca agctcctcga ggtcggcccc
     4141 aggttcctcg atgcgcagga aggcgaggtt gtggtcctga cgcacctcga cgctcatcag
     4201 ggagtgctgt agcgtcgggt cagggacccc gccgatagtc cgtcgcagcc cgttgtgttc
     4261 gacgacgcgc ctgaccgctt ccagataagg tggcggagga tagatctcgc gctccggttc
     4321 gtctgtgatg cgcatgtaga acagggcgac ggacccgcga cgaccttctc tctcctcgcc
     4381 gatctgcttg tacgagacgg acgcaggaat atagcctagc aagaaccccg tctcccgcgc
     4441 gccgagatga agattgcccc tctggctgta cggatggatg gcggtcgcct cgctgtagag
     4501 cccgtacatg ccggcgtcag cggcccactc cgccatgaac gttttcattc tcgggaagag
     4561 atggtgtcca cgaaaccgtg ggtccacgac cgcctgtccg gactccccca caagcgagcc
     4621 gggacgttcc acggtaatcg ctagatgccc cacgaactcg ccctccggcg ttaccgcgac
     4681 gcaggagatc attagcccct tctcctgaag ctccctgatc ctgtcaggat agtagatgta
     4741 gtcccagtcg tagctgtagc cgtaggagcg gtagacgcag cgcgaaagct cgaatgattc
     4801 atcaggacgc atcatgcgaa tctccagagg gacgtcctca tgcgcagcgg cggcttccac
     4861 ggttccgcgg tgctcctctt cggggaggtg gtccctgacg tcggtgtgag gaaggtgctt
     4921 gagtaactcg accctgttgc cgcgccggcc gaggttgata aatcgcgtct cgtcgaagga
     4981 cctgtgaagt atctccagca cggtccgatc ctcaccgtcc tgcaggcgtt tgtagtcgaa
     5041 gggcagcccc cgatcctcga ccgccacgac cacaagaccc ggtcggcgca ggacctggac
     5101 gtcgaaggaa ccctcctctt caggtccgaa ggcgtgctcg ataatgttcg tgcaaaccag
     5161 ttcgacggcg tgatccactt gctcggcgtc ccccaggccg agccgatggg taatctgctt
     5221 cacgaagtcg atcaggggtg gtaagaaccc ggtatctgct gataccgtga cgcgggcgat
     5281 cgattcctcc ggcgttgctt tcatctcggt catgagtaat ccttcttcga aacagccatt
     5341 tgcatcagga acttgattct ctcaggtctc gtcgtcctca gccaaacatg cagcacgcac
     5401 aagaagctga caagctcccg acaggacgcg tgctgagatg ctgaccagct tgcattaagt
     5461 aaagttaagt gagatcgttg acaagttctc gctggggggg tagagttcta gcgatgttgt
     5521 tgtcgcgaga aggactgaag gattgatgca catccgtcgc acgaacatag gtgttccggt
     5581 gttcggggga gaggtgcgtc ccgaggtccg tgaggtcgcg atgagaacgt gagcttctag
     5641 ctcaaccaca cttctcagga gaggcccccg gaccgagcgg accgggggcc tcgagctttg
     5701 ctccgagaga gctggcgaag gtctttggct ccgatcccaa cacggacggt atcagccctt
     5761 ccagggcgcg acgaagagac gagagaagga gacagagaga tgttcaacga ctggcactac
     5821 gtcttggcac aggaccggga agcccgtgaa caagacttcg ccgaaagggt ggccgagcgc
     5881 gggagatacg cgcggtcccc tggacggccc tccctgaggg ccagggtagc gcgcaggctc
     5941 ttcgccctgg cggtggccgc tgagagcgag gagacctggc gcgtcgtttg ggagaggttg
     6001 gaggccaggg ggcgcctgta gagtgggcgc ccctcagggc gggatttcta taggaggttc
     6061 ttatgaaggc tctggagcta agaggagtag agaagagttt caagaagcgg cgtggggagg
     6121 tggtacgcgc cctcgactcg atctcgctct cggtcgggaa aggggaggtc gtcggcatcc
     6181 tcgggcccaa cgggagcggg aaaagcacgc tggtgcgtgt tatctccacg ctcgtcctcc
     6241 ctgacagcgg agaggtcgag gtcttcggga tcgacgccac gcgccatccc aagcgggtgc
     6301 agcgcagcat gaaccgggtt agcgtcgagg caagcttctt caaa
//
LOCUS       DASTKM010000067         9587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1422193, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000067 DASTKM010000000
VERSION     DASTKM010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422193"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..486
                     /locus_tag="VFQ10_02625"
     CDS             289..486
                     /locus_tag="VFQ10_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964266.1"
                     /translation="MEQRKSEAEVALELMRLVLEHAGSRRLSPTRTLALYHLCKEAVA
                     YSPEDRVTADYLEENLKKLLQ"
     gene            787..1713
                     /locus_tag="VFQ10_02630"
     CDS             787..1713
                     /locus_tag="VFQ10_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964267.1"
                     /translation="MSVIEDGRVETGLEGRGARCLALDPKDPDTLYVGTSDEGIFKSE
                     DGGGTWEKLSGIGHPRVTAVAVSPTDGAVYAGTEPSALFVSRDGGLSWRELEGMRNLP
                     SAPTWSFPPRPWTSHVRAIALSYADPDLVVAGIELGGVVRSADGGETWQDQRPGAQAD
                     CHSLFAHPGAPELLYEAGGGGFAQSTDFGKSWEGADGGMDLHYVWGLAADAEDPSLVY
                     TSAASGPGRAHGARPSNATIYRRSGNERWQPVLEGLFAFPYALCQDPQTPGTLYAGFG
                     DGTILRGTDAGAQWEEVAQVASGIQDLASVST"
     gene            complement(1812..2090)
                     /locus_tag="VFQ10_02635"
     CDS             complement(1812..2090)
                     /locus_tag="VFQ10_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9964268.1"
                     /translation="MDLPRLRRIRQGAVMSQEELAERSGVARDTISKLETGRRGAYPS
                     TIRKLAAGLEVRPQMLMGGVEYLDEPSEGEPTDKKPVEKPERDKRIGF"
     gene            2193..2822
                     /locus_tag="VFQ10_02640"
     CDS             2193..2822
                     /locus_tag="VFQ10_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET9964269.1"
                     /translation="MSLDPRLVVPYVTEQSPRGERTMDIYSRLLADRIIFIGMPVDDQ
                     VANVVMAQLLHLDSEDPEQDINLYINSPGGSVHAGMAIYDTMQFVNADVATTALGMAA
                     SMGSFLLTAGAKGKRSALPNARILLHQPHIGGGLGGQVSDVEIHARELVRTKQRMNEL
                     YSQMTGQPIEKIERDTDRDYMLGPEEALEYGVIDRVVNRPQEGVIAAGK"
     gene            2845..3837
                     /locus_tag="VFQ10_02645"
     CDS             2845..3837
                     /locus_tag="VFQ10_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9964270.1"
                     /translation="MDLLILGGTGFLGRHLVEAALGDGHRPALFNRGLSEPGLFPEVE
                     KLEGDREGNLSALLGRRWDAVIDTCGYVPRVVRASAGLLADAVEHYTFVSSISVYSDA
                     IAPGADEEAPVRELPDPTVEEVTEETYGGLKALCERAAEEMLPDRVLNVRPGLISGPH
                     DPTDRFSYWPRRVAAGGEILAPDHEERGVQYIDVRDLASWMVKMSEERRTGTYNATGP
                     DYELRMGRLLEECEAVGGAGAEPVWVSEDFLEEKGVEPFTELPLWLPREYAALQTIDC
                     GRAIEAGLTFRPLSETIEDVLDWDRATRAGREPAAGLRPEREQELLRAWHGATL"
     gene            3853..4260
                     /locus_tag="VFQ10_02650"
     CDS             3853..4260
                     /locus_tag="VFQ10_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964271.1"
                     /translation="MNLTHEYMHHRTGYRLGAGCCWIRIYKGAECDAPVVVCEELPKV
                     GGVATGEVSGYLAAEVVREHFSGGLPDLPRPLLWIEHRPARRRGPGKYFLHTFPSYRP
                     RTVGAGFVRRVTLGASQREPLAPAEVAILIREV"
     gene            4408..4596
                     /locus_tag="VFQ10_02655"
     CDS             4408..4596
                     /locus_tag="VFQ10_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964272.1"
                     /translation="MGEEKGTTEYAVKQVGDRYYPVIIDRDAGGHYEIKNPLTGGVLS
                     YNNPEAAEKFVQRALEKG"
     gene            4777..6402
                     /locus_tag="VFQ10_02660"
     CDS             4777..6402
                     /locus_tag="VFQ10_02660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9964273.1"
                     /translation="MSGSRTSEKEPVRAYRRGTPSGRAVRERRFREPPLRSLLESRAL
                     LVLLEIMLLGAVLRFYGLGFQSLWSDELASWDISTRETISQVIGGVRGDDHPPLYFLI
                     LRFAQWIFGDSEWALRLPSAFAGWLCIPAIYLLGKMLYSEREGIVAALFLAVFWAPIY
                     FSQEARVYSMLILLSILTFYFWWSVMLGLRYRRELPTGESALYVVCAVLCAYVHYFGL
                     ILVVLQGAALAALAYGTLRKAMLLYVPVTVAYLPWLPSMVHQFGYNTQSGTLTGEPTL
                     QVPPDYFQFLFGRSGLLSFAAWTLLSFLLIRGWDDLRRRRKRGVVPPGLLLAVWALGP
                     FVVAIVASQSMLTNENLLVSLPAVYLLLARSVTRTFSGRAAAVFQGTVAVGLAAVGLA
                     YLLFSMDYYTTPTKEQIRKAALFVTGHEGKNTLVVRCDTDNRLDYYLRIRESGARDDV
                     QACEAGDFQKIESRVKEGDYKEVFHLISHTDPDQQMVSEFQRSFQPVRYERFDGAAVV
                     VYKVRTSMPEGLPQPEPPDVPQPEPPGNLPRQE"
     gene            6439..7365
                     /locus_tag="VFQ10_02665"
     CDS             6439..7365
                     /locus_tag="VFQ10_02665"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET9964274.1"
                     /translation="MESKERSGAVGLKGVLAAVLTPMDEDLNPDHQAFAAHCHRLLAA
                     GCHGLSVFGTTGEANSLSVNERLAALEALIESGVPVEKLLPGTGSCAITDTVWLSRAA
                     LEAGTAGALVLPPFYYKDVGDDGLFRFFAEVVERVGDDRLRLYLYHIPQMTGVDLGLP
                     LISRLIEAYTGVVVGTKDSSGERERIMTLCREFPDFSVLAGTETFLLETLRSGGEGCI
                     SATVNVTSRLARRVHDAHALGKDDEAEALQERLTQLRASIEAYPVIPALKQLMATLTG
                     DKQWRNIRPPLSSLDEKHTNALLSNVPLAELL"
     gene            complement(7454..7615)
                     /locus_tag="VFQ10_02670"
     CDS             complement(7454..7615)
                     /locus_tag="VFQ10_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964275.1"
                     /translation="MLVGDAQGSKGMAEAIDHAQSILESVLREAKTAGAENAARSGIF
                     GRVLYLYVP"
     gene            9072..>9587
                     /locus_tag="VFQ10_02675"
     CDS             9072..>9587
                     /locus_tag="VFQ10_02675"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9964276.1"
                     /translation="MKREARTDKFTEEVEDRYAGYTVYDRSYEKIGSVDDLFVDENDS
                     PEYIGVKMGFLGTRTSLIPFHIARVNDARQIIEVAADKETLKNGPTFDDDREITPEFE
                     NEVYFYYGLERSSTIEQSGTHGAYYAEQSTQTTGVADPTDVSDEDEVRVQRAEEEIRV
                     GTRERKAGSIRV"
BASE COUNT         1899 a         2869 c         3027 g         1792 t
ORIGIN      
        1 cgtctgaggg gcggccgggg atctttggac agaggtcggc ctccctgccg acggtgacgt
       61 aggtgctcgc gtaagtggcc ctgaaccggt ggccgcagac gggacagacg acgtaaggtc
      121 tgctcggctc acccacaggc atcgtcagcc tcccgcgtgc ctccgggccg cgtatctgag
      181 tcccttctgg ggcctggaac tgggatcaaa gcgctcccga ttctaccata gctgctcgct
      241 ccgccggtat aattccctgc acagaactgc ccggagggag gcatccgtat ggagcagcgc
      301 aagagcgagg ccgaagtcgc cctggagctt atgcggctcg tcctggaaca cgctgggagc
      361 aggcgactct ctccgacccg tactctggcc ctctaccacc tctgcaagga ggccgtcgcc
      421 tactccccgg aagaccgcgt aaccgcggac tacctggaag aaaacctgaa gaagttgctt
      481 cagtagcatt tcagcaactc agcacttccg catctcagct tgggcgaagt cactgtcggc
      541 gaggtcatac cttcctgtgc ctgaaggact ttgtgtcttg tcctggttgg ggacagggag
      601 gatcgagggc ggagcgcata gtcaggagta cctatttccg aaaggtcgga acgccaggcg
      661 accaggtagg gtgcctgacg ccttccactt cggccttgta tgtctgtgag cgtcatcgca
      721 tactctcacc ggtacgaaaa aggaggtcta gccgtggtac gactgtacgt ggcaggagaa
      781 aatgctgtgt ctgtgataga ggacggtagg gtcgagaccg ggctggaagg gcgcggcgcc
      841 cggtgcctgg cgttggaccc caaagatcca gacaccctct acgtcggcac ctcggacgag
      901 ggtatcttca agagcgagga cggaggaggg acgtgggaaa agctctccgg catcggacac
      961 cccagggtca ccgccgtcgc cgtctcgcct accgacgggg ctgtctacgc ggggacagag
     1021 ccctcggccc tgttcgtcag ccgcgacggc ggtctatcct ggcgcgagct ggagggcatg
     1081 aggaatcttc cctccgcgcc aacctggagc ttcccaccca ggccatggac ctcccacgtc
     1141 cgtgctatcg cgctctccta tgcagaccca gatctcgtcg tggccggcat agagctgggc
     1201 ggggtggtga ggagcgccga cggcggtgag acctggcaag accagaggcc tggtgcccag
     1261 gccgactgcc attccctgtt cgcccacccc ggagctcctg agctcctgta cgaggccggg
     1321 ggcggtgggt tcgcccagag cacggacttc ggcaagagct gggagggcgc agacgggggg
     1381 atggatcttc actacgtgtg gggcctggcc gcagacgcgg aagacccctc gctcgtctac
     1441 acttcggcag cctcgggacc tggccgggcg cacggcgcaa gaccatcgaa tgcgaccatc
     1501 taccgcaggt caggcaatga gcggtggcag cctgtcctcg aaggcctgtt cgcattcccc
     1561 tatgcgttgt gccaggaccc acagacacca gggacgctgt acgctggctt cggagacggg
     1621 acgatactcc gcggcacaga tgccggggcg caatgggagg aagtcgccca ggtagcctcc
     1681 gggatccagg atctcgcgtc agtgtcaacc tgaaacaggc cgatagcact tcagcaagtc
     1741 agcacgccgc cctgcgggcg gttcgtcaac ttcctgggcg gttcgcgaac cgtccctaca
     1801 agaagatcgc tctagaatcc gatcctcttg tccctttccg gcttctctac gggcttctta
     1861 tcggttggct ccccttcgga cggctcatcc agatactcca cgccacccat cagcatctgt
     1921 ggccgtactt cgagaccggc ggcgagcttg cggatcgtag aaggatacgc cccccttcgt
     1981 cccgtctcga gcttcgagat cgtgtcccgc gcaaccccac tcctctcagc caactcctcc
     2041 tgactcatca cagccccctg ccgaatgcgc cgcaaacgcg gaaggtccat actcagtacc
     2101 tccaaaacac tgcaaaaaac ttgcacaaaa cctacaacaa tgttacgcta attggagtat
     2161 agcgtgttgg cggaagtaga gggagtttgg tgatgagttt ggatccgagg ctggtcgttc
     2221 cctatgtgac ggagcagagt ccgcgtgggg agcggactat ggacatctat tcgcggcttt
     2281 tggcggacag gatcatcttt atcgggatgc cggttgacga ccaggtcgcc aacgtcgtga
     2341 tggcccagct cttgcacctg gattctgagg accccgagca ggacatcaac ctgtacatta
     2401 actcccctgg tgggagcgtg catgctggga tggcgatcta cgataccatg cagttcgtca
     2461 acgcggatgt ggcgacgacg gcgcttggga tggcggcctc gatggggtcg ttcctcctta
     2521 cggcgggggc gaagggcaag cggagcgcgc tccccaacgc gcggatactg ctgcaccagc
     2581 cgcacatcgg cgggggtctc ggggggcagg tctcggacgt ggagatccac gccagggagc
     2641 tcgtaaggac caagcagcgg atgaacgagc tgtattccca gatgacgggg cagcccatcg
     2701 agaagatcga acgcgacacc gatcgcgact acatgttggg acctgaggag gccctggagt
     2761 acggtgttat cgacagggtc gtaaaccgtc cgcaagaggg cgtcatcgcg gcaggcaagt
     2821 agagcctgtg cgcatatagc acggatggac ctactcatac tgggcggaac gggattcctc
     2881 gggcgccacc tcgtcgaggc ggcgctcggg gacggccatc ggcctgcact cttcaaccgg
     2941 gggctgagcg agccagggct gttccccgag gtcgagaagc tcgagggcga cagagaaggg
     3001 aacctctcag ccctcttggg acggcgctgg gatgcggtga tcgacacctg cggctacgtg
     3061 cccagggtcg tcagggcgtc tgccgggcta cttgctgatg ctgtcgaaca ttacaccttc
     3121 gtctccagca tctccgttta ctctgacgcc atcgcgccgg gggccgacga agaggcgccg
     3181 gtgcgggagc tcccagaccc gacggtcgag gaggttacgg aggagactta tggggggctc
     3241 aaggcactgt gcgagcgggc cgccgaggag atgctgccgg acagggtgtt gaacgtccgt
     3301 ccaggtttga tctctggtcc gcacgacccc acggaccgtt tttcctactg gccccgccgg
     3361 gtcgctgccg ggggcgaaat acttgcccct gaccacgaag agcgtggagt gcagtacatc
     3421 gacgtgcgag acctagcgtc gtggatggta aagatgtcgg aggagcgccg gaccggaacg
     3481 tataacgcca cgggccctga ctacgagtta cggatgggcc gattgctgga agaatgcgaa
     3541 gccgtcggtg gcgccggcgc ggagccagtc tgggtgtccg aggacttcct ggaggagaag
     3601 ggagttgagc cgttcacgga gttgccgttg tggttaccgc gggagtacgc cgccctgcag
     3661 accatagatt gtggcagggc catcgaggcg ggactcacct tccgacccct gtccgagacg
     3721 atcgaggacg tgctggattg ggaccgcgcc acgagggcgg gtagagagcc tgctgcgggc
     3781 ctcaggcccg agagggagca ggagttgctc cgggcctggc acggtgccac cctgtagaat
     3841 gcgggttgcg agatgaacct cacgcacgaa tacatgcatc acaggacggg ctacaggctc
     3901 ggggccgggt gctgctggat caggatctac aaaggcgccg agtgcgacgc tcccgttgtc
     3961 gtgtgcgagg agctccccaa ggtcggaggc gtcgctacgg gagaggtgtc aggttatctg
     4021 gccgccgagg ttgtcaggga gcacttctct ggtgggctgc cggatctgcc ccgtccactg
     4081 ctctggatcg aacaccgtcc ggcccgcagg cgcgggccag gcaagtactt cctgcacacc
     4141 ttcccctctt accgtcccag aaccgtaggc gcaggcttcg tgcggcgcgt tacccttgga
     4201 gcctcgcagc gcgagcccct cgctccggca gaggtagcca tcctcattcg ggaagtctag
     4261 ggtgactggc aggaggattg acataccgcg aatcgctcgc tcggctttga gccatcggct
     4321 atgagcgaaa acaggaagct gaactgctga caagccgatt gcggttacaa tgaggatgtc
     4381 ctctttgcaa ccaacccagg aggcgaggtg ggcgaagaga agggcacgac cgagtacgcc
     4441 gtaaagcagg tcggagaccg ctactatcct gtcatcatcg acagggatgc aggcggtcac
     4501 tacgagataa agaaccctct gaccggcggc gtcctgagct acaacaaccc cgaagccgca
     4561 gagaaattcg tacagcgcgc ccttgagaaa gggtaggggc ggttcgcgaa ccgccctgct
     4621 gcggtttgcg agcagcccta tatatcgtgc ccactcacgc agtccccaca atggtttgtc
     4681 taggatcgga ggagacgttg atgtacctgg agaacctgtt ctaacatccc ataacgtccg
     4741 ctgctcgtaa ccgtgcgtga aaggggagcg cgtacagtgt caggatctag gacgagcgag
     4801 aaagagccag tacgcgccta ccgccgtggg acaccctcag gaagggcggt tcgcgaaagg
     4861 cgctttcgcg aaccgcccct ccgctccttg ttggagagca gggcgcttct tgttctgctg
     4921 gagattatgc tcctcggtgc ggtgttgcga ttctacggcc tgggcttcca gtcgctctgg
     4981 agcgatgagc tggcgagctg ggacatcagc accagggaga cgatctccca ggtcatcggg
     5041 ggagtgcggg gcgacgacca tcctcccctt tacttcctga tcctccgctt cgcgcagtgg
     5101 atcttcggcg attcggagtg ggctttgagg cttccttctg cctttgcggg gtggttgtgt
     5161 ataccggcga tctaccttct cggcaagatg ctctactcgg agcgcgaggg gatcgtagcg
     5221 gcgctcttcc tcgcggtatt ctgggctccc atctacttca gccaggaagc ccgcgtttac
     5281 tcgatgctga tcctgctctc catcctcacc ttctacttct ggtggagcgt tatgctcggc
     5341 ctgcgctatc gccgggaact gccgaccgga gaatccgccc tttacgtcgt ctgcgccgta
     5401 ctctgcgctt acgtgcacta cttcgggctg atccttgtcg tgttacaggg cgcagcactg
     5461 gcggcgctcg cgtacggcac gctgcggaag gcgatgctcc tttacgtccc cgtaaccgtc
     5521 gcttacctgc cctggctgcc tagcatggtc caccagtttg ggtacaacac acagagtggg
     5581 acactgaccg gggagccgac cctacaggtc ccgcccgact acttccagtt cctgttcggc
     5641 cgctcggggt tgctctcgtt cgccgcgtgg acgctgctct ccttcctcct catccgcggt
     5701 tgggacgacc tgcggcgtcg caggaagaga ggcgtagtcc caccaggctt gctgctcgca
     5761 gtgtgggccc tgggaccgtt cgtcgtggcc atcgtggcgt cgcagtctat gctcacgaac
     5821 gagaacctcc ttgtctcact acccgccgtc tacctcctcc ttgcacgctc cgtaacccgc
     5881 acgttctcgg gcagggcggc ggcggtcttc cagggaaccg tggccgtcgg gctggccgcg
     5941 gtgggccttg cgtacctgct gttctcgatg gactactaca ccaccccgac caaggaacag
     6001 atccgcaagg ccgccctctt cgtcacaggc cacgaaggca agaacactct ggtcgtccgc
     6061 tgcgacacag acaacaggct cgactactat ctgaggatca gagagtcggg tgcgagagac
     6121 gacgtacaag cctgtgaggc cggtgacttc cagaagatag aaagcagggt gaaagaagga
     6181 gactacaagg aggtcttcca tctaatctcg cacacagatc cggaccagca gatggtctcc
     6241 gagttccaga ggagcttcca gccggtccgc tacgaacggt tcgacggcgc cgccgtggtc
     6301 gtctacaagg tcaggacgtc catgcccgaa ggcctgccgc aacccgagcc ccctgacgtg
     6361 ccgcaacccg agcctcctgg caacctcccg aggcaagaat gaccctggag agcgtgtgga
     6421 ccgggcagag aggagaccat ggagagtaaa gagagatcgg gcgcagtggg gctgaagggt
     6481 gtcctggcag ccgtcctcac cccgatggac gaggacctaa acccggacca tcaggctttc
     6541 gccgcccact gccacaggtt gctcgcggcg ggctgccacg ggctctcggt cttcggcact
     6601 acgggtgagg caaactccct ctccgtgaac gagcggctcg cggcgctcga agcgctaatc
     6661 gaaagtggtg tacccgtaga gaagttactg ccagggaccg ggtcctgcgc catcactgac
     6721 accgtatggc tgagccgcgc ggcgctcgag gctgggaccg cgggtgcgct cgtactgccg
     6781 ccgttctact acaaggacgt aggggatgat ggtctgttcc gcttcttcgc cgaggtcgtc
     6841 gagcgcgtcg gcgacgaccg tctgcgcctc tacctctatc acatcccaca gatgacaggc
     6901 gtcgacctcg ggttgccctt gatctcacgc ctcatcgagg cctacaccgg cgtggtagtc
     6961 ggtacgaagg acagctccgg tgagagggaa cgcatcatga ccctctgccg ggaattccca
     7021 gacttctccg tcctcgccgg cacggagact ttccttctag aaacgctgcg tagcggcggt
     7081 gaaggctgca tctccgcaac cgtcaacgtc acctcccgcc tcgctcgccg cgtacacgac
     7141 gcgcacgcat tggggaaaga cgacgaggcg gaagccttgc aagaacgcct aacgcaactg
     7201 cgcgcgtcca tcgaagcata tcctgtaata ccggccctca aacaactcat ggccacactg
     7261 accggcgaca agcaatggcg caacatccgt cccccgctct ccagcctgga cgaaaaacat
     7321 acgaacgccc tcctctccaa cgtgccccta gccgaactgc tctaagagcc tcgtgcatgg
     7381 cttgtcaggt ctcgcacacg ctacaggctg aaatgctcgt cgggcattag gcttaatgat
     7441 cggttgtttg ttgttagggg acatagaggt agagcactct gccgaaaata cccgatctag
     7501 cagcgttctc agcaccggcg gtcttggctt cccgcagaac gctctccagt atcgattgag
     7561 catgatcgat ggcttcagcc atccccttac tgccctgagc gtctccgacc agcatagtct
     7621 ggccctcctc tttcccctac ctgccggatc gacgggggca tcgttgctgg tttggcttct
     7681 aagggcctgg tcgccccggt cctgatggtc tagtatcccc ctcgtgggag aagggcgtac
     7741 agagggcgca caacccgacg agtctacaaa ggagtatgtg actctcggag aggccgccaa
     7801 actcctcggg atccatcgga acaccgtaca caacgggatc aaggttgctg aaggtgaatg
     7861 tgagggttac ggtatagagc gagattcgct cggcggtggg cgcacaagtg cagatgtgca
     7921 ttaggcgccc actgcacaac cgctagtaag gaggtcttcc gaatgatagc gacccgcttg
     7981 aacgagatcc tgtcggacta cacacgagtt gggcgccgtc agggaacagt tcggtgccga
     8041 gtgagtccgc cgcgaaatgg cggagaccac gctgcgcgag ggaatggcgg aagagcaacg
     8101 ccggcgggag caggccgaac gggagcgtga cgatctctgt caggacctag aggccatcat
     8161 ggcggcgcga gaatcgcctc agacgggcga ggaacagcag agtagagtcc gccccactgc
     8221 gttacagtag aggctcagga aggcttacag aggtcctgat ggcgcaagcg gatagggagg
     8281 tgatggcgcg atgcaggaac gacgtgagca gctgctacaa gagctacgca aggcagagca
     8341 ggcacgagac ttgctgagtg aactggagcg tctcctaaag gaagaaaatc attcggaact
     8401 aaggcgctgc gtgacgcagc tgaagcgttt ggcgcaatcc ttgcctgagg gccacagcgt
     8461 gcggctgttg atcgaatctg ctatccggcc tgagatggtg ggcagtgtaa gtagagctcg
     8521 ctctgcctta gagggagaag tcaggacgct tcagggagcc ctgcactacc actccagcta
     8581 atcgcccgga ttttatcgga tcggagtaag aggcttcgcc gataggtggc tcctcatggc
     8641 ctgcggtggg gtcgactttc caaacggtag cctctccagg cttctcggat tcgctgctta
     8701 cggtaattat cacctcggag aaggcggccc acaagtcgat gacccttcgt tacctctgtg
     8761 tacaccagtt tcttgtggac gttgtgccag gtgttaattc gctctaccac gcaacgcttt
     8821 gtggcctgaa acggagccgg cttgcccttc tcggaaatca tgggctccag gccacgggac
     8881 tttagtttct ggcgggtggt ctgcgaatcg tagccacgat cgaggtagac gctcatctgt
     8941 tcggggagca acccaagagc atctccaggt gtccagggtc tctcccaaca gcggcgttta
     9001 gctttgccag gagagtgggt agagctagca atgtatggtc gtacgaagca tcctatgtgg
     9061 aggtatttac cttgaaacgc gaagcgcgca ctgacaagtt cacggaggaa gtagaggatc
     9121 gctacgcagg ctacacggtc tacgaccgaa gctacgagaa gatcggcagt gtagacgacc
     9181 tgttcgtaga cgagaacgat agccccgagt acataggcgt gaagatgggc ttcctaggga
     9241 cccgtacttc cctgatcccg ttccatatag cgagggtcaa cgacgcgcgg cagatcatcg
     9301 aggtcgcagc agacaaggag acgctcaaga acggaccgac tttcgatgac gaccgtgaga
     9361 tcacccccga attcgagaac gaggtctact tctactacgg cctcgagcgt tcttctacca
     9421 tcgagcagag cggcacccac ggagcctact atgccgagca gagcacccag acgacgggtg
     9481 tggccgatcc caccgacgtg agcgatgagg acgaggtgag ggtgcagaga gcagaggaag
     9541 agattagggt cggaacccgc gaacgcaagg ctggctcaat aagggtc
//
LOCUS       DASTKM010000068         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1246877, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000068 DASTKM010000000
VERSION     DASTKM010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246877"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VFQ10_02680"
     CDS             complement(<1..862)
                     /locus_tag="VFQ10_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET9964277.1"
                     /translation="MALEQERTTPPSADASPGGEGRLRKASVLLGGLAVFALVLVLYL
                     KTLAPTVLYLQDPKLLDAVMLQMQVSVLGITHPTGYPTYLMLTHLFTYLPFGDPAYRV
                     NLGSAVYAALAVVAVYAAGLLLGRRVVAAASGALAFGLGTALWSQAVIAEVYTLNALL
                     VSATILVLLLWREYRKDRYLLLSAFLLGLCLTNHLTSGLLLPASLLLVALVDWRRLVD
                     VRLVVRGAGLFLLGLTPYLYLPLRAAMDPPMEANNPTDFGRFWYVVSGGNLTGSFFSF
                     GPSELPERMVF"
     gene            1156..>2245
                     /locus_tag="VFQ10_02685"
     CDS             1156..>2245
                     /locus_tag="VFQ10_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9964278.1"
                     /translation="MKVLVLGGDGYCGWPTSLHLSDVGHEVVIVDNCVRRTIDHELSG
                     ASLTPISDIKDRVGAWREVSGQTIGFEFGDLLDWEFVSDVVQRHQPEAIVHFAEQRAA
                     PYSMIDRRHAVYTQHNNVIGNLNVLFAINEYVPDCHLVKLGTMGEYGQPNIDIEEGYI
                     EIEHNGRKDLLPFPKQPGSWYHLSKVHDSHNIHFACKIWGLRATDLNQGVVYGTVTQQ
                     AERDGRLINRFDYEEVFGTVLNRFCVQAAIGHPLTVYGNGSQTRGYLDIRDTVRCIEL
                     AVENPAERGEMRVFNQFTEEFSVRQLAELVVDAANKLGIEARMESIENPRVEKYDHYY
                     NAKHTGLLDLGLEPHLLGDSLLDSLLNIA"
BASE COUNT          497 a          662 c          710 g          376 t
ORIGIN      
        1 agaagaccat cctttccggc aactccgaag gcccgaagga gaagaacgag ccggtcaggt
       61 ttcccccgct gaccacgtac cagaaacgcc cgaagtcggt agggttgttc gcttccattg
      121 gagggtccat cgcggccctg aggggcaaat acaggtaggg agtgagcccg agcaggaaga
      181 gcccggcacc ccttacaacg agcctgacat cgactagcct gcgccagtct acgagggcga
      241 ccaagagaag gctggctggc aagagcaatc cgctggtgag gtggttggtc aggcagagcc
      301 ccaaaaggaa ggccgagagc agcaggtaac ggtctttgcg gtactcgcgc cagagtagca
      361 ggacaaggat ggtggcggac acgagcaggg cgttgagggt gtatacctcc gcgataacag
      421 cctgggacca gagggccgtc cctagcccga aggccagggc gccagacgcc gcggccacca
      481 cccgccggcc gagcaggagc ccggccgcgt agacggctac cacggccagc gccgcgtaga
      541 cggcggaacc gaggttcacg cggtaggcag ggtccccgaa aggcaggtac gtgaagaggt
      601 gcgtcagcat caggtaggtc ggatagccgg taggatgcgt gatgccaagc accgagacct
      661 gcatctgtag catcaccgcg tcgagcagct tggggtcctg taggtagagg acggtgggag
      721 ccagagtctt gaggtagagg accagcacga gcgcgaagac cgcaagccca ccgagcaaga
      781 cactagcttt gcgtagacgt ccctctcctc cgggagacgc atccgcagaa ggaggagtag
      841 tcctttcctg ttccagtgcc actatctaag acgtcctttg ccgatcttcc gggcgaagct
      901 attgtaccca gatgggctac cgatgttaat ttgcattaag ttgcgttaat atagcgtgcg
      961 gcgggtatgc taacgggtgt atcgtcagag ctatgcgagg ccggcgtgcc ggaccgcagg
     1021 aagagcggaa cagagcgttt acaccgattt agtacgcgtc taacgatttt tccgctacaa
     1081 tcgtcggctg gcaagttctg tttgaattgg gtcagatccc ataagagttt gtagagcaag
     1141 aagggagcga gagtaatgaa ggtactggtt ctaggcggcg acgggtattg tggctggccg
     1201 acctctttgc acctttccga cgtcggccac gaggtggtga tcgtggacaa ctgcgtcagg
     1261 cgtaccatcg accacgagtt aagcggtgcg agccttaccc cgatctcgga cataaaggac
     1321 cgggtcgggg cgtggcgcga ggtcagcggg cagaccatcg gttttgagtt cggcgaccta
     1381 ttggactggg agttcgtctc cgacgtcgta cagcgccacc agcccgaggc catagtgcac
     1441 ttcgccgagc agcgggccgc tccctactcc atgatcgaca ggcgccatgc cgtctacacc
     1501 cagcacaaca acgttatcgg caacctgaac gtgctcttcg ccataaacga gtatgtgcca
     1561 gactgccacc tagtgaagct cggtactatg ggcgagtacg gccagcccaa catagacatc
     1621 gaagagggct acatcgagat agagcacaat ggccgcaagg acctcctgcc cttcccgaag
     1681 cagccaggaa gctggtacca cctcagcaag gtccacgact cccacaacat ccacttcgcg
     1741 tgcaagatct ggggcttgag ggccacggac ttgaaccagg gcgtcgtcta cgggaccgtc
     1801 acgcagcagg ccgagcgcga cgggcgcctc ataaaccgct tcgactacga ggaggtcttc
     1861 gggacggtcc tcaaccgttt ctgtgtgcag gcggccatag gccatccgct cacggtctac
     1921 ggcaacggtt cccagacgag gggctacctc gacatccgcg acacggtgcg ctgcatcgag
     1981 ctcgccgtgg aaaaccccgc cgagcgcggc gagatgcggg tcttcaacca gttcaccgag
     2041 gagttctcgg tcaggcagct cgccgagctg gtggtggacg ccgcgaacaa gctcggcatc
     2101 gaggccagga tggagagcat cgagaacccg cgcgtcgaga agtacgacca ctactacaac
     2161 gccaagcaca cgggcctcct cgacctcggc ctcgaaccgc acctcttggg cgacagcctg
     2221 ctggactcgc tcctgaacat cgcgc
//
LOCUS       DASTKM010000069         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1247188, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000069 DASTKM010000000
VERSION     DASTKM010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247188"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..103
                     /gene="ffs"
                     /locus_tag="VFQ10_02690"
     ncRNA           4..103
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFQ10_02690"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            430..2232
                     /gene="dnaX"
                     /locus_tag="VFQ10_02695"
     CDS             430..2232
                     /gene="dnaX"
                     /locus_tag="VFQ10_02695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET9964279.1"
                     /translation="MNHTTLYRRWRPRRFGGIVGQEPVVRTLRRAIEMDRVSHAYLFS
                     GPRGTGKTSTAKVLAMGLNCEKGPTPEPDGTCQSCRAIVSNSSMDVLEMDAASNRGID
                     EIRDLRDKVNLAPVAGRMKVYIIDEVHMLTAEAFNALLKMLEEPPEHVIFVLATTEKH
                     KVLPTIISRCQSFDFRRPSIETLTEKLREISDAEGIEVEPEALTVIARQGRGSFRDAE
                     GLLDQLSSFAEGRITASMVRELLGGVGPESLIETTYALHERRAADALRIVDRLSGEGK
                     DLGQFVAELLAHLRNLMLLPYAPEVALAEVGAEERPQLEEQANAVPTAEVVRLIEALD
                     DAVSLMRRGADPKLELELVFVKLTRDYTEPDMGAILGRLESLERAVENGSVATNPPRA
                     TADTVREEPPAEREPAHETVEPDAPSDDASLLEDSDDRPETGVDLASEWHGIMGELKR
                     RRQALTAAVYGEARVESFDGSVLRLIYPEEQSFHVGMAKDPGHLHKLGEVLEERLGSA
                     PRLEINAAGEASATTATEEQRPTPPPIEQPAPASEESPPKTGSPNGSGSTVAENSGPQ
                     QEAMRRDDVIHDQREVFEIAREFGLFDENERAKS"
     gene            2579..>2710
                     /locus_tag="VFQ10_02700"
     CDS             2579..>2710
                     /locus_tag="VFQ10_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET9964280.1"
                     /translation="MQQVQQMQQQMQQAQEELANETVTASAGGGAVKATMTGSMELVS
                     "
BASE COUNT          542 a          816 c          896 g          456 t
ORIGIN      
        1 gatggcctgg tcccgggcaa tggggccccg tgaaccgtgt caggaccgga aggtagcagc
       61 actaagcggg taacctcatg tgctccgggg tagccagacc ggagtcggcg gccggaagtt
      121 cgccgggtgc ggttacgtcg aaggcggggt gcgcggcatt tcagcatttc agcacgctcc
      181 gcccttcggg cttcgcttgt cagcacggcg ccctgcaggc gccttgtcag catttcagcc
      241 tctctcgttc cggcggtact cgttgcgttc caccaacccc tcttcagcct gccgccgaca
      301 tctgggtttg tacttacggg cagagtcgcg cgctggtaca aaaagctgaa gtgctgacac
      361 ccccgatggt tcgagacgag tcgtccggta tactaaatgt gtgagtcact cgaaccgaag
      421 ttacttctaa tgaaccacac gacgctctac cgcaggtggc gtccgcggcg ctttgggggc
      481 atcgttgggc aggagcctgt tgtacggacg ttgcgacgcg ccatcgagat ggaccgcgtc
      541 tcgcacgcct acctattctc tggtcctagg gggacgggga agacgagcac ggcgaaggtg
      601 ctcgcgatgg gcctcaactg cgagaagggg cctacgccgg agcccgacgg gacttgccag
      661 agctgcaggg ccatcgtgag caactcctcg atggacgttc tcgagatgga cgcggcctcc
      721 aaccggggga tagatgagat acgggacctc agggacaagg tgaaccttgc gcctgtcgcc
      781 ggcaggatga aggtctacat catagacgag gtccacatgc tcacggccga ggccttcaac
      841 gccctcctca agatgctcga agagcccccc gagcacgtca tcttcgtgct cgccacgacc
      901 gagaagcaca aggtcttgcc gacgattatc agccgctgcc agagcttcga cttccgccgc
      961 ccgagcatcg agacgcttac ggagaaactg agggagatct cggatgcaga aggcatcgag
     1021 gtcgaacccg aggccctgac cgtcatcgcg cgccagggga ggggctcttt cagggacgcg
     1081 gaggggcttc ttgaccagct ctcgtctttc gcggagggta ggatcacggc ctccatggtg
     1141 cgcgagttgc tgggcggcgt cggccccgag tcgctcatcg agacaaccta cgccctgcac
     1201 gaacgcaggg cagcggacgc cttgcgcata gtggaccgcc tctcgggcga ggggaaggat
     1261 ctcggccagt tcgtcgctga actgctcgcg cacctgagaa acctgatgct cctcccttac
     1321 gcccccgagg tcgcactcgc cgaggtcggg gccgaggaac gcccccagct cgaagagcag
     1381 gcgaacgccg tccccacggc cgaagtcgtg cggctgatcg aagcacttga cgacgccgtg
     1441 agccttatgc gacgcggcgc agaccccaag ctggagctgg agctggtctt cgtcaagctc
     1501 acccgcgatt acacggaacc ggatatggga gctatcctgg gccggctgga gagcctcgaa
     1561 cgtgcggtag agaacggaag cgtcgccacg aaccccccgc gggccaccgc tgataccgta
     1621 cgtgaggagc ctcctgctga gcgagagcct gcgcatgaaa cggtggagcc ggacgcgccg
     1681 tccgacgacg cttcgctcct cgaagactct gatgaccggc cggagaccgg cgttgacctc
     1741 gcctccgagt ggcacgggat catgggcgag ctcaagcgtc gccggcaggc gctcacggcc
     1801 gcggtctacg gcgaggcgcg ggtcgagagc ttcgacggta gcgtgctgcg gctcatctac
     1861 cccgaggagc agagcttcca cgtcgggatg gccaaggatc cgggacacct ccataagctg
     1921 ggcgaggtcc tcgaggaacg cctcggctcg gcaccacgcc tggagatcaa tgccgcggga
     1981 gaagcatcgg ccacgaccgc cacagaagaa cagcgcccga cgcccccccc gatagagcaa
     2041 ccagcgccgg cttctgaaga gagccctcct aagaccggga gtccgaacgg gtcaggcagt
     2101 acggtcgctg agaatagcgg tccccagcag gaagcgatgc ggcgcgacga tgtgatccac
     2161 gaccagcgcg aggtcttcga gatcgcccgc gagttcggtc tcttcgacga gaacgagcgc
     2221 gctaagagtt aggaattgtt ggcttactct cgactgtttt cgaatccata cagttgcgct
     2281 ctggagcccg cgcaagactg aatctcgact gaagatatag catcccacag gtgaagcgcg
     2341 gcgtgagcga gatggaggca ggtttaagtt ctaccggggg agcctgtata cgaggttggc
     2401 cgctacgaac cgagagtaga gtcgcatgga aggctccgtc aggaatttcg tgctactttg
     2461 cgaagggtgc gggggccaaa ggtggtggcc tgcctggtta gatcgcttat actcatgtgg
     2521 tccatagacc tagagccagg gagtgttcgt ggggaatcgc agaaatgtaa acaagatgat
     2581 gcagcaggtt cagcagatgc agcagcagat gcagcaggct caggaggagc tcgcgaacga
     2641 gacagttacc gcgagcgccg gcggtggggc tgtgaaggcg acgatgaccg gcagcatgga
     2701 gctcgtctcc
//
LOCUS       DASTKM010000070         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1424290, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000070 DASTKM010000000
VERSION     DASTKM010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424290"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..240)
                     /locus_tag="VFQ10_02705"
     tRNA            complement(167..240)
                     /locus_tag="VFQ10_02705"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Ile,seq:gat)
     gene            393..893
                     /locus_tag="VFQ10_02710"
     CDS             393..893
                     /locus_tag="VFQ10_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET9964281.1"
                     /translation="MSANGFTKMSIYGMNMDLFSSSPIVILKVEDENRYLPIWIGQAE
                     ARSILFKLQNQEFVRPLTHDLALSLVNELGGSMERITVTELRDSTFFATIRLEIEGKT
                     VEVDSRPSDAIALAIRSGAEIFASDEVIEEAGVVFEEAIEDTPEDEVVDKFKDWMNQV
                     SPEDFK"
     gene            complement(955..1233)
                     /locus_tag="VFQ10_02715"
     CDS             complement(955..1233)
                     /locus_tag="VFQ10_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964282.1"
                     /translation="MDLLMVRDRSTGRFVYTERLERTSGETSWEYVRRSVRREARIRT
                     RFDGDAMEVIVGWDVDSVDAFLRAYPEYRTDGDFDGASGTQEHEGRSG"
     gene            complement(1236..2303)
                     /locus_tag="VFQ10_02720"
     CDS             complement(1236..2303)
                     /locus_tag="VFQ10_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964283.1"
                     /translation="MAEHAAEYEWRGEGEEAEVVLYAPDDSAFERTLPAARLPGVESP
                     VYAAASHVDLGWAAASATHVAPDLLSAPLRGLLLVAGTRSENLGVPPQELMQRVLREL
                     PEVGARLPSLNESGAGRFCESGARSAAEDGLIEEEDLPLLDVRPGDADAIGRRALAAA
                     PREWEGGVEPDLRLVTGVLDTDGAESLGLEEGALVLVVRVDAGDLGLLALETHRERTR
                     LGDFGVTPELPAAPAGSGEADDLVAALYAAANFADARASRALWMLRRVLGGAAGGLEI
                     CAAWRTGGVEVREGRMVHRRDLAWAESGRVLVCGSYAAAGTGNMWASAPPFGVPEEEG
                     RWPWEEAGLLERLAMLHPPEG"
     gene            complement(2312..>3095)
                     /locus_tag="VFQ10_02725"
     CDS             complement(2312..>3095)
                     /locus_tag="VFQ10_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET9964284.1"
                     /translation="AGEELPFSQRDISWRGHSIEVRLNAEDASNDFAPSPGVVTAYEE
                     PGGPGVRVDSSLRGPGVVAESYDPLFAKLIVSGVDREEALTRLGRALAEFRVEGISTT
                     LPFYRAILDDEAFVSGNYTTGFIAERMQRLNIQPSTKGETGEASEKRVREVEVEVNGK
                     LFRVRVFGDEDATGGPPRRRRGETRRASTSEGAVRAPMQGTIVKVLVKEGQEVAADEP
                     VCILEAMKMESEVRSQKAGTVSEVRVEAGQTVRSGDTLIFVE"
BASE COUNT          567 a         1081 c          906 g          541 t
ORIGIN      
        1 cgcacttcct gccgagcatg ggggaccaga ccgtaaaggc catggaagcg gcgctttcgt
       61 agggcaaaac cgtgttgctg cacggttgct gcacaggggc cccgggtacg tctcaaggcc
      121 cgcttcgcac ttcatgtttt acctgcaaaa cacaagattt tcgtcgtggg cctgcctgga
      181 ctcgaaccag ggacctcttc cttatcagag aagcgctcta accggctgag ctacaggccc
      241 taagtgatcg gtgcaacaga tcttcgccgc cggaataatg tacaacacgg gcaggcgact
      301 gacaacctga ttgaacacaa gagaggctgg cagtaaaatc ggccagtata ggaaaacccg
      361 cgacctcgcg gagtgagagg agcatatagc cagtgagcgc caacggcttc accaagatgt
      421 ccatctacgg catgaacatg gacctctttt cctccagccc aatcgtcatc ctcaaggtcg
      481 aagacgagaa ccgctatttg ccgatctgga tcggccaggc tgaggcccgc tctatcctgt
      541 tcaagcttca gaatcaggaa tttgtcaggc ctctgacgca cgatcttgcc ctcagcctcg
      601 tcaacgagct gggcggctcg atggagagga ttacggtaac cgaactgagg gactcgacgt
      661 tcttcgccac cataaggctc gagatagagg gcaagacggt cgaggtcgat tccaggcctt
      721 ctgacgccat tgccctcgcc atccgatcgg gggccgagat cttcgcctct gacgaggtaa
      781 tagaagaggc cggcgtcgtc ttcgaggagg cgatagaaga cacccccgag gacgaggtcg
      841 tagacaagtt caaggactgg atgaaccagg tctctcccga ggacttcaag taggggcggt
      901 tcgttgtaag ggcgcttcgc gaagcgccct taggccatca tacgggtacc gcctctaccc
      961 acttcgccct tcatgttcct gcgtccccga cgccccgtcg aagtctccgt ccgtacggta
     1021 ctcggggtat gcgcgcagga acgcatcgac cgagtcgacg tcccacccga cgatgacctc
     1081 catcgcgtcc ccgtcgaagc gcgtccggat acgggcctcc ctcctgacgc tacgccggac
     1141 gtactcccag gacgtctcgc cggatgtgcg ctccagcctc tccgtgtaaa cgaaccggcc
     1201 cgtgctccgg tcccttacca ttaacaggtc caacgtcagc cctccggggg atgtaacatc
     1261 gccagacgct ccaacagccc agcctcttcc cacggccacc ggccttcctc ctccggaacc
     1321 ccgaacggcg gcgcactcgc ccacatatta cccgttccgg ccgccgcgta acttccgcag
     1381 acaagcacac gcccactctc ggcccaggcc agatcccttc tatgcaccat ccgcccttcc
     1441 cgcacctcga ccccaccggt cctccaggcg gcacagattt ccagccctcc cgccgcaccg
     1501 cccagcaccc gcctcagcat ccacagagcc cttgaggcgc gggcgtcggc gaagttggcc
     1561 gcggcataga gcgccgcgac gaggtcatcg gcctcaccgc tgccggcagg ggctgccggc
     1621 agttcgggcg taacgccgaa gtctcccaac cgagtgcgct ccctgtgggt ctcgagggcg
     1681 agcagtccga gatctcctgc gtcgaccctg acgacgagga cgagggctcc ctcctccagc
     1741 cccagactct ctgcgccatc ggtgtcgagg acccccgtca ccaacctgag atctggttcc
     1801 accccaccct cccattcgcg cggagcggcg gcgagcgccc ggcggccgat ggcgtcggcg
     1861 tccccagggc gcacatccag aagcggcaga tcctcttctt cgatgaggcc gtcctccgca
     1921 gcggaccgcg cccccgactc acagaatctg cccgcccctg actcgttcag ggaaggcagg
     1981 cgtgcgccga cctcggggag ctcccgcagg actctttgca tcagctcctg tggcggcacg
     2041 ccaaggttct cggacctggt accggcgacg aggagcaggc cacgcagtgg ggcggaaagg
     2101 aggtcggggg ctacgtgcgt ggccgaggct gcggcccatc cgagatctac gtgcgaggcc
     2161 gcggcgtaga cggggctctc cacgccgggg agtctggcgg cgggcagggt gcgttcgaaa
     2221 gccgagtcgt cgggagcgta gaggacgacc tcggcctcct cgccctcacc gcgccactcg
     2281 tactccgcag cgtgctcggc caaaggacac cctactctac aaagatcagg gtgtcccctg
     2341 accttacggt ctgcccggct tcgacgcgga cctcggagac ggtgccagcc ttctgggagc
     2401 ggacctcgct ctccatcttc atggcctcga ggatgcacac gggctcgtcc gcggcgacct
     2461 cctgcccctc cttcacgagc accttgacga tggtgccctg catcggtgcg cggaccgcac
     2521 cctcgctcgt cgaagcccga cgcgtctcgc ctcgcctgcg ccgcggaggc ccgccggtgg
     2581 cgtcctcatc cccgaacacc ctcaccctga agagtttccc gttcacctcg acctcgacct
     2641 cacggacgcg cttctcagag gcttcacccg tttcgccttt ggtagagggc tgtatattta
     2701 gtcgctgcat ccgctcggct ataaagcccg tcgtatagtt gccggagacg aatgcttcgt
     2761 cgtcgaggat agcgcggtag aagggcaacg tcgtggagat accctccacc ctgaattcgg
     2821 cgagcgcccg cccgagccgc gtcagggcct cctctctgtc gacgccgctg acgatgagct
     2881 tggcgaacag cgggtcgtag gactcagcca cgacgcctgg accgcgcagc gaggagtcta
     2941 ctcttactcc tggcccgcct ggttcctcgt aagccgtaac gacaccagga ctcggggcga
     3001 agtcgttcga cgcgtcctcg gcgttcaggc gcacctcgat ggagtggccc cgccaagaga
     3061 tgtctctctg cgagaaggga agttcctcgc cagcg
//
LOCUS       DASTKM010000071         7328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_14852, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000071 DASTKM010000000
VERSION     DASTKM010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14852"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /gene="aroA"
                     /locus_tag="VFQ10_02730"
     CDS             <1..945
                     /gene="aroA"
                     /locus_tag="VFQ10_02730"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET9964285.1"
                     /translation="LGRGPFTVDGVPRMRERPVADLVDGMRQLGAEVEYAGEDGRFPL
                     MIKGGGIPGGKARVSASKSSQFISGLLMAAPYAQAPVMLHPVGRKEWPYVGITVALMR
                     AFGVEVDEANGRFTVAPALYSSRAYEVEPDASGASYFMAAAAVTGGRVRIPGLGASSP
                     QGDLRFAGVLRDMGCRVEIASDFVEVQGPDHLRGVEVDMNAFSDTMITLSAIAPFAEG
                     PTTIKNVGHTRLQESDRLSAVATELTRLGVKTQTTSSSIRIIPKNIRSGVVRTYGDHR
                     MAMAFAITGLVASGIRIRDPGCVTKTFPGYFGALESLR"
     gene            980..1645
                     /gene="cmk"
                     /locus_tag="VFQ10_02735"
     CDS             980..1645
                     /gene="cmk"
                     /locus_tag="VFQ10_02735"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET9964286.1"
                     /translation="MGGILVAIDGPAGSGKSSVARAVAGRLGVVNLNTGAAYRAAALL
                     ALREDVDLADGEGLAALARRVELDPEGASIDGGRVGESELRSPEVSAAASKVSAHPPL
                     REVLLPVQRAAAQKARAKGGAVVEGRDIGTVVLPDAELKVYLSAAPEERARRRAYQTG
                     REGELDRIRAALARRDRQDSEREISPLKPASDAVVLDTTSLSLEEVISRVLDLATDLR
                     EQT"
     gene            1642..2295
                     /locus_tag="VFQ10_02740"
     CDS             1642..2295
                     /locus_tag="VFQ10_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9964287.1"
                     /translation="MRGAMLFEGRRKPGEMSGRYRLFRWVMTLLCRLLFGYRVHGGNS
                     VPSDGPLIVASNHSQYADPVLVCVAVPRRLQWMAKKQLFVFPFRKFFEFIGSFPVDRE
                     GGGRGAIRAALAFLAEGWALGIFPEGTHRGAGASREAKSGVVVLALRSGASVLPVHIG
                     KLPGPLSRLRGQRLHIFVGEPVHLDPAMRGGAAYREAADEILHTIYALPERRAQESL"
     gene            2292..3596
                     /gene="der"
                     /locus_tag="VFQ10_02745"
     CDS             2292..3596
                     /gene="der"
                     /locus_tag="VFQ10_02745"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET9964288.1"
                     /translation="MNRLPVVAVVGAPNVGKSSLVNRIMGRRGAVVSERPGTTRDRAY
                     NRAEWAGKEFTLVDTGGMEPRAKVGLETLVTLQARVAVEEADVVVHLTDARLGPTEAD
                     AVIAGELKRARSKVVLAVNKLDNPADDTERHSFYSLGEGEPHAISAVHGLGVGDLLDV
                     VVSVLPQSEAQSEEEPPALAIVGRPNVGKSTLLNRLLGTDRAVVSEKAGTTTDAVAAE
                     IALQVEGETERFLLLDTAGVSKGARRARGVPYYSSLRTSEAIRRSDVSLLLVDATDGI
                     VAGDLRIAREIEEAGSSCGVLVNKRDLVPPQRLREIEGLISARLTDLKPQAVHISALT
                     GAGVDEVLPFAARVAQAYTKRVPTHEVAEFVGEVVGRSAPPKGVKIRYATQTGTAPPT
                     FTLFATRPKDLPENYLRYVENALRERYGLWGVSVRIKVKSSR"
     gene            3761..6277
                     /locus_tag="VFQ10_02750"
     CDS             3761..6277
                     /locus_tag="VFQ10_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9964289.1"
                     /translation="MKAIIMAGGQGTRLRPLTSDQPKPMIPIVNSPCMEHIVKLLERH
                     GFTDIAVTLQFLPDEIRDYFGDGSEWNVNIRYSVEDAPAGTAGSVKMAEQQLGLQGER
                     LLIISGDALTDADLSTLVEFHEEKGSEATMVLKSVANPLDFGIVITEEDGRISRFLEK
                     PAWGQVFSDTVNTGIYLLEPSATAEIPDPEGGEYDFSKELFPKLLDAGRPLYGYITED
                     YWEDIGTLEQFASAQRDVLDGRIEGIRPPGTRLRDNIYVGRRAQVDDDELEGPVVIGE
                     NVRVDEGAWIGPYSVIADNVVVSAGASVERSVIAEGTYIGEGAELVDTLVGRNSYIQA
                     RARILERSALGDDVIVGEGATIAPEVKVYPHKTIETGANVTQSLIYETMGLRTVFRRG
                     VVSGKFNVDVTPEFVTRLASSFGTTLDPGAVVTLGRDSTLSAQVAKRAMTAALLGTGI
                     NIRDLRAAHAGVVRHDVLAGKSSAGAHVRAGSDPDDVEILFFSADATPITEGDERSVE
                     KTFVREEYRRAYGGDIGELIYPGRVVEQFVERLERAVDREKTTGATIVVDFCGGVAGL
                     VASRVFSRLGVSAVVMEGFANANAVGEAGRMNLEDSIERVARIVPTVDAAFGAVVGPE
                     AEYVQFVDDEGEWVPPDVMLACLIDQMHPQTAVLPINLSRGYERLVDQGQGKVEMSRT
                     GLSNVAIRAASIRADLASLADGRYIFPAFLPAPDCFMTLASALEVFHEMPLSGVRRKF
                     GDTFDHVHREQIECPWSAKGRVMRGLAEKFGGDPDAILAEGVLLNLEGGWVLMLPDPD
                     SPMFYVYAESPDGEGNPAQLMEVYVDLVKSLIDEQALLSS"
     gene            6316..6573
                     /locus_tag="VFQ10_02755"
     CDS             6316..6573
                     /locus_tag="VFQ10_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964290.1"
                     /translation="MDHSGMHGGFDWENAEDVTALSEEDLRDRLEALSEEERALGYRR
                     RILQGRIDLIRGELVERGAVALSPRDLARVLLGDSPDKETR"
     gene            6570..7019
                     /locus_tag="VFQ10_02760"
     CDS             6570..7019
                     /locus_tag="VFQ10_02760"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9964291.1"
                     /translation="MRKCPECGAEGSAGMIFCAECGSKLDVGQSTVSYAPSFGDDVEP
                     GTSGGAEGAALIEMDQVEGTAGRRMHDLGDDVVSVGRGQESSIFLDDVTVSRKHAEIV
                     HGDGGYRIRDVGSLNGTYVNRVRVDAVDLRNGDEIQVGKYRFRFVFS"
     gene            7075..>7328
                     /locus_tag="VFQ10_02765"
     CDS             7075..>7328
                     /locus_tag="VFQ10_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9964292.1"
                     /translation="MNREKGEGYAPASESYSATKDSFTIGEVVERLRPEFPTLSVSKI
                     RYLERRRLINLSRTRGGYRLFSSQDIELLRYILTLQDKEYL"
BASE COUNT         1412 a         2189 c         2468 g         1259 t
ORIGIN      
        1 cttggcaggg gcccgttcac cgtggacgga gttcccagga tgcgcgagcg cccggtcgca
       61 gatctcgtag acgggatgag gcagctcggt gctgaggtgg agtacgcagg agaagacgga
      121 aggttcccgc ttatgataaa gggcggcggg atccccggcg ggaaggcgcg cgtgtcggcg
      181 agcaagagca gccagttcat cagcgggctg ctgatggcgg caccgtacgc acaggctcct
      241 gtcatgctcc accccgtggg caggaaagaa tggccctacg tcggcatcac agtggcgctg
      301 atgcgggcct tcggcgtgga ggtagacgaa gcaaacgggc gcttcaccgt cgccccagcc
      361 ctttactcct cgcgggcata cgaggtggag cctgacgcct cgggggcgtc ctactttatg
      421 gcggcggcgg ccgtaactgg cgggcgcgtg aggattccag gtctcggcgc atcttcgcca
      481 cagggagacc tgcgcttcgc aggcgtgctg cgcgacatgg gctgtagggt cgagatagcc
      541 tctgacttcg tcgaagtaca agggcctgac cacctgcgcg gcgtcgaagt cgacatgaac
      601 gcgttctctg acaccatgat cacactctct gcgatcgcac ctttcgctga aggccctacc
      661 accataaaga acgtaggaca tacgcgtctg caggagtcgg accgcctctc cgccgtcgca
      721 accgaactga cccgcctcgg tgtaaagacg caaacaacct catcatctat ccgcataata
      781 cctaagaaca taaggtctgg agtggtacgc acttacggcg accacaggat ggcgatggcg
      841 ttcgcgataa cggggctggt tgcgtcgggc atcaggatcc gggaccctgg atgcgtgaca
      901 aagacctttc ccggttactt cggggcgttg gaatccttga ggtgaagcgg cgggactttc
      961 ggctatactt cctgcgtcga tgggaggcat actcgtagcc atagacggac ccgcggggag
     1021 cgggaagagc tcggtcgcgc gggccgttgc cgggcgcctc ggggtcgtca acctcaacac
     1081 gggcgccgcc tacagggccg ccgcgctcct tgccttgcgc gaggatgttg accttgccga
     1141 cggggagggg cttgctgccc tcgcgcgcag ggtggagctg gatccggaag gagctagcat
     1201 cgacgggggg cgggtggggg agagcgaact ccgctcgccc gaagtctcgg cggcggcatc
     1261 gaaggtctcg gcccatcccc cgctcaggga ggtcttgctc cccgtacaga gggcggcggc
     1321 ccagaaggcc agggcaaagg ggggtgccgt cgtcgaggga agggacatcg gtaccgtcgt
     1381 cctgcctgac gctgagctga aggtttacct atccgccgcc ccagaagagc gggcgcgcag
     1441 gcgggcatac cagacgggca gggaggggga gttagatcgc atcagggccg ccttggccag
     1501 gcgtgaccgc caggactcgg agcgggagat ctcacctctc aaacccgcct cggacgccgt
     1561 agtcctcgac actacgtcct tgagcctcga agaagtcata tcaagggtcc tcgacctcgc
     1621 cacagatcta cgcgagcaaa cgtgagggga gccatgctct tcgagggaag gcgaaaaccg
     1681 ggtgagatgt ctgggaggta ccgcctgttc cggtgggtga tgaccctgct ctgccgcctc
     1741 ctgttcgggt acagggtcca tgggggtaac agtgtgcctt ccgatggacc cttgatagtg
     1801 gcctccaacc acagccagta tgccgacccc gtcctggtct gcgtggccgt cccccgtcgc
     1861 ctgcaatgga tggcgaaaaa gcagctcttc gtcttccctt tcaggaagtt cttcgagttt
     1921 atcgggtcgt tccctgtgga ccgcgagggc ggaggaaggg gcgcgatacg cgccgccctg
     1981 gccttcctgg ccgaaggttg ggcgttgggc atcttccccg agggaaccca cagaggcgcc
     2041 ggagcctcca gggaagccaa gagcggtgtc gtcgtactcg cactccgcag cggcgcttcc
     2101 gtactgcccg ttcacattgg caagctacct ggacccctct cccgcctgcg cggccagcgg
     2161 cttcacatct tcgtgggcga accggtgcac ctggacccgg ctatgcgggg tggtgcggcg
     2221 taccgcgaag cggcggatga gatcttgcac accatctacg ctctgccgga gcggcgtgcg
     2281 caggagtcgc tttgaaccgg ctccctgtgg tcgccgtcgt cggggccccc aacgtgggga
     2341 agagctcgct ggtcaacagg atcatgggcc gacgcggtgc cgtagtctct gagaggccgg
     2401 gtaccacgcg tgatagagcg tacaaccggg ctgagtgggc cggcaaggag ttcacgctgg
     2461 tggacactgg cgggatggag ccgcgcgcga aggtcggcct cgagacgctc gtgacgctgc
     2521 aggccagggt tgcggtcgag gaggctgacg tggtggtgca cctcaccgac gccaggctag
     2581 ggccgaccga ggccgacgcg gttatcgctg gcgaactcaa gcgcgcgcgg agcaaggtcg
     2641 tgctcgcagt caacaagctg gataaccccg ctgacgatac cgaacgccac agcttctact
     2701 ccctcggtga gggtgagccc catgcgatct cggcggtgca cggcctgggt gtcggggacc
     2761 ttctcgacgt cgtcgtctct gtgctgcccc aatcagaagc acaaagcgaa gaggagccgc
     2821 cggcgctcgc catcgtcggc aggccgaatg tcggcaagag tacgcttctc aatcgactgc
     2881 tcgggacgga ccgcgccgtc gtctcggaga aagccggcac gacgaccgac gccgtcgcgg
     2941 ctgaaattgc acttcaagta gagggagaga ccgaacgctt tctcctgctc gatacggcag
     3001 gtgtaagcaa gggcgccagg cgggcccgcg gcgtgcctta ctactcatcc ttgcggacct
     3061 cagaggcgat aagacgttcc gacgtctcgc ttctgctggt ggacgccacg gacggcatcg
     3121 tggcaggtga cctcaggatc gcccgcgaga tagaggaggc aggctcctcg tgcggggtcc
     3181 tggtcaacaa gcgggatctc gtacccccac aaaggctgcg ggagatagaa ggactcatct
     3241 cggcccgcct aaccgacctc aagccgcagg ctgttcacat ctcggcgctc acgggtgcgg
     3301 gggtggacga ggtcttgcct ttcgcggcta gagtggcgca ggcatacacg aagcgggtgc
     3361 cgacgcacga ggtagcagag ttcgtgggtg aggtggtagg gcgcagcgct cccccgaagg
     3421 gggtcaagat ccgctacgca acccagacag gcacggcgcc accgacgttc acgctcttcg
     3481 ccaccagacc caaagatctg cccgagaact atctgcgcta cgtggagaac gcgctccgag
     3541 agaggtacgg actctggggc gttagcgtca ggataaaggt caagagcagc aggtagagag
     3601 accatttggg ccactttgcg caaactccca gcgcgtgtag ggtagacttt gattgggaga
     3661 gctagggcag aggaggttga gcagagaaca tgttcgacgt ggcggctaga tcagggcact
     3721 tgaggcgttg gcggtttgtt ccggccagga aggtcctccc atgaaggcga tcatcatggc
     3781 cggggggcag ggaactcgcc tgaggccgtt gaccagcgat cagcccaagc cgatgattcc
     3841 cattgtcaac tccccttgca tggagcacat cgtgaagctc ctcgagcgcc acggcttcac
     3901 ggatatcgcc gtgaccttgc agttcttgcc ggatgagata agggactact tcggcgacgg
     3961 ttcggagtgg aatgtaaaca tccgctactc cgtcgaggac gccccggcgg ggaccgcagg
     4021 ctccgtgaag atggccgagc agcaactcgg cctccagggc gagcgcctgc tcatcataag
     4081 cggcgacgcc ctgacggacg ccgacctgag cactctggtc gagttccacg aggagaaagg
     4141 ctctgaggcc acgatggttc tcaagagcgt cgcgaacccc ctcgatttcg gcatcgtcat
     4201 aaccgaggag gacgggcgca tctcgcgctt tctggagaag ccggcctggg ggcaggtctt
     4261 ctccgacacg gtaaacaccg gcatctatct gctcgagccc tcggcgacgg ccgagatccc
     4321 cgaccccgaa ggaggagagt acgacttctc caaggagctg ttccccaaat tgctcgacgc
     4381 aggacgtcca ctatacggat acatcaccga ggattactgg gaggatatag gcactctgga
     4441 acagtttgcc agtgcccagc gcgacgtgct cgacggccgc atagagggca tcagacctcc
     4501 gggcacgcgc ctgagggata acatctatgt ggggcggagg gcgcaggtag acgacgacga
     4561 gctcgaaggg cccgttgtga tcggggagaa cgtccgcgtc gacgagggcg cgtggatcgg
     4621 gccctactcg gtcatcgccg acaacgtcgt cgtctcggct ggcgcctcgg tggagcgcag
     4681 cgtcatcgcc gaggggacct acataggcga gggcgctgag ctcgtggaca ccctcgttgg
     4741 tcgtaactcc tacatccagg cgcgggcgcg cattctggaa cgcagcgcgc tcggtgacga
     4801 cgtaatcgtc ggcgagggag cgaccatagc ccctgaggta aaggtgtacc ctcataagac
     4861 catagagacc ggggccaacg tcacgcagtc tctgatctac gaaaccatgg gccttcgcac
     4921 cgtgttcaga cgcggcgtag taagcggcaa gttcaacgtg gacgtaacgc ccgagttcgt
     4981 cacccgcctg gcctcatcct tcggcaccac gctcgacccg ggggccgtcg taaccctagg
     5041 tcgcgattct acgctctcag cccaggtcgc aaagcgcgcc atgacggcgg cgctgctggg
     5101 gacggggatc aacatcaggg acctcagggc agcccacgcc ggggttgttc gccacgacgt
     5161 gctcgccggc aagtcttcgg ccggggcgca cgtccgtgcc ggcagcgacc ccgatgacgt
     5221 agagatcctc tttttctccg ccgacgctac ccccataaca gagggagatg agaggtctgt
     5281 ggaaaagacc ttcgtgcgcg aggagtaccg tagagcctac ggcggggaca taggggagtt
     5341 gatctacccc ggccgcgtcg tggagcagtt cgtggagcgc ctggaacgcg ccgtggacag
     5401 ggagaagacc acaggcgcca ccatcgtcgt ggatttctgc gggggcgtcg ccggcctcgt
     5461 ggcgagccgg gtgttctcca ggctcggcgt gagcgccgtc gtgatggaag gtttcgccaa
     5521 cgccaacgcg gtcggcgagg cgggaaggat gaacctggag gatagcatcg agcgcgtcgc
     5581 ccgcatcgtg cccacggtgg acgctgcctt cggcgccgtc gtcggcccgg aagccgagta
     5641 cgtgcagttc gtcgacgacg agggagagtg ggtgccccct gatgtcatgc tcgcctgcct
     5701 catcgaccag atgcacccgc agaccgccgt gctccccatc aacctaagcc gcgggtacga
     5761 aaggctcgtg gatcagggcc agggcaaggt cgaaatgagc cgcacgggcc tctccaacgt
     5821 cgccataaga gccgccagca tcagggctga cctcgcttcc ctggccgacg gccgttacat
     5881 cttccctgcc ttcctgcctg ctcctgactg ctttatgacg ctggccagcg ccctcgaagt
     5941 attccacgag atgccccttt ccggcgtgcg ccggaagttc ggagacactt tcgaccacgt
     6001 tcacagagaa cagatcgagt gcccgtggtc ggcaaaagga cgtgtgatgc gcggcctcgc
     6061 cgaaaagttc ggcggggacc ccgatgccat cctcgccgag ggcgtgctgc tcaacctcga
     6121 aggcggctgg gtgcttatgc tcccagatcc cgacagccct atgttctacg tctacgccga
     6181 gtccccagac ggcgaaggga acccagcgca gctaatggaa gtgtacgtcg acctggtgaa
     6241 gtccctgatc gatgagcagg ctctgctctc aagctgagat tgcacttcag gttcagggta
     6301 ggttatactc agaccatgga ccattcgggg atgcatgggg gattcgactg ggagaacgcc
     6361 gaggacgtca cggccctctc tgaggaagat ctgagggata ggctagaggc gctctcggag
     6421 gaggagcggg ccctcggtta caggcggagg atcctgcagg ggaggataga tctgatccgt
     6481 ggcgagctcg tagagcgggg tgctgtcgcg ctttcgccca gagacctggc gcgggtattg
     6541 ctcggcgatt cgcccgataa ggagaccagg tgagaaagtg tcctgagtgt ggagctgagg
     6601 gctcggcggg gatgatattc tgcgcggagt gcggctccaa gctcgatgtc ggccagagca
     6661 ccgtctcgta cgcgccgagc ttcggcgatg acgtcgagcc tggcacatca ggcggcgccg
     6721 aaggagccgc cttgatagag atggatcagg tcgagggtac cgccggacgc aggatgcacg
     6781 acctgggcga tgacgttgtc agcgtcggcc gtggacagga gagcagcatc ttcctcgacg
     6841 acgtcaccgt ctcacgcaaa cacgcggaga tcgtgcatgg agacggcggt tacaggattc
     6901 gcgacgtggg aagcttgaac gggacatacg tcaaccgagt cagggtcgac gccgtcgacc
     6961 tgcgcaacgg agatgagata caggtaggca agtaccgctt caggttcgtg ttctcctaga
     7021 gcggcaggca ctgtagtcga gaggatggga gtgaacaggg tggagaacaa cggtatgaat
     7081 agggagaaag gcgaaggtta cgcgccagcg tcggagagct acagcgctac caaggacagt
     7141 ttcactatcg gcgaggtcgt cgagcgtctg agacccgagt tcccgaccct ctcggtcagc
     7201 aagatccggt acctggaacg caggcgtttg atcaacctca gccgcacgcg cggcggttac
     7261 aggcttttct cctcccagga tatcgagctc ctcaggtata tccttacact gcaagacaag
     7321 gagtatct
//
LOCUS       DASTKM010000072        12226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1072316, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000072 DASTKM010000000
VERSION     DASTKM010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072316"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFQ10_02770"
     CDS             <1..466
                     /locus_tag="VFQ10_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET9964293.1"
                     /translation="ADQMVQGLGLAESTPGPLIMVTQYVGFIGAWNDPGPFSPLLYGT
                     LGALITTYATFLPCFLFIFFLAPYIELLANDRRLRAILIGVTAAVVGVVANLAVYFAT
                     QVLFPEGISLVGLDIFALVVAVVSFVVLQRYKVPIYLMVPVGGVLGMAWTLL"
     gene            482..997
                     /locus_tag="VFQ10_02775"
     CDS             482..997
                     /locus_tag="VFQ10_02775"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HET9964294.1"
                     /translation="MKRPHWVLLVYRIPREPSRHRVAVWRKLRDLGALYLQDGVAALP
                     EDAATREQLEWLQLRVREAGGEATLWEAKPGTMAEEAELVGAFRSSREEAYRAIIAGA
                     ERIRRKAEMGGRALSEQLGKLEREFRAERRRDYFRSPLRLEAAGALKAAREAIRDRER
                     SPGVLGTEEKV"
     gene            997..1413
                     /locus_tag="VFQ10_02780"
     CDS             997..1413
                     /locus_tag="VFQ10_02780"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HET9964295.1"
                     /translation="MLWATRRGCHVDRTACIWLIQRFLDPQATFDFFRDPAEAPEGAE
                     PFDVVGARLSHRGEDCSFETFLKEYELDDPVLREIAEIVHDADLMDEKYGRPESEGLD
                     AIVRGMQLSLPDDHALIEHTDATFDGLYAYLRRETL"
     gene            1415..2125
                     /locus_tag="VFQ10_02785"
     CDS             1415..2125
                     /locus_tag="VFQ10_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9964296.1"
                     /translation="MYRPKIEVRGLRYEAGGTEILAGVDALVPAGEVTAVVGPSGAGK
                     STLLRAINRLIEPSSGEVYLDGESTGSLDPLELRRRVGMVFQIPALFGVSVEEAVLYG
                     ARLAGRSADPARLLEVVGLDPSMCRRVPETLSVGQQQRVSVARALALEPEALLLDEPT
                     SALDEGARRKIEELVRDLNARLGLTMVFVSHDLSQVERVADRVVLLAEGRSAGQWERP
                     EFFSTAGERARRLIAGRL"
     gene            2136..2900
                     /gene="fetB"
                     /locus_tag="VFQ10_02790"
     CDS             2136..2900
                     /gene="fetB"
                     /locus_tag="VFQ10_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HET9964297.1"
                     /translation="MQTVGSALLTLGLVAIAVALSLYEKLGLEKEIGVAVFRSFVQLA
                     AIGYVIDFIFGLESIGAVALLLAGMVLFAAWTSASRAGDVPRAFVVVALAVGVAAVAT
                     LGLLLALGVIPATARYLIPLGGMAIGNSMNAASLTLTRIRDDVREQRPKVEAALALGA
                     TGRQAASPILKTALQSALIPLIDATKTTGIIFLPGAMVGMIIAGANPLEAVRLQIVVL
                     YMLLGSVSIAAILVGTLSYRSFFTPRHQIRKAMSDE"
     gene            complement(3097..3924)
                     /locus_tag="VFQ10_02795"
     CDS             complement(3097..3924)
                     /locus_tag="VFQ10_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9964298.1"
                     /translation="MTNGDDTRAPEAGRYASVNGLEMYYEIHGSGPPLVLLHGAFSAI
                     GTSFGELLPALAETRQVIAFELQGHGRTADIDRPLSIEQMADDTAAALRHLGIAQADI
                     FGYSMGASVALHLAIRHPDVVRNLVLASVTYNLSGVHPGLMEGLADMKPEMMFGSSWH
                     EEYTRIAPNPENFATLFAKKTQMDRNIKDVPAEVVQSIKAPTLLIIGDSDLVRPEHAV
                     EMFRLLGGGVFGDTPAGLPNSQLAVLPGTSHVTVVHRADLLLSIIPPFLDAPMPVAE"
     gene            4221..4667
                     /locus_tag="VFQ10_02800"
     CDS             4221..4667
                     /locus_tag="VFQ10_02800"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9964299.1"
                     /translation="MKQLHTQIEIDAPAKRVWELLTDFASYPQWNPFIRHISGRLAPG
                     ERLQARLEPPGGRAMTFKPKVLAAEPDRELRWLGHLLVPGVFDGEHSFTIEPLEENRV
                     RFVQREAFKGLLVPLFARSLETNTQRGFEEMNRALKERAETAPAQD"
     gene            complement(4706..5959)
                     /locus_tag="VFQ10_02805"
     CDS             complement(4706..5959)
                     /locus_tag="VFQ10_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964300.1"
                     /translation="MSPRISALLAGSAWALSAAFVLLSVPLYWSTSASVVLLGVPQIV
                     AVLALAALVLAFSLVGALIVARRPGNGVGWILCAAGLTIGATTLASGYAVFSLTGPSG
                     LPATEWAAWFAYWIWVPGVGPAMTFGLLMFPDGRLPSRRWRVVGWLSVVALVTLACGL
                     AFTPGPLSDYPEIDNPVGLAPLEGSVLEDGGFGWGLLSASVVLSAVSIAVRYRGATGE
                     ERQQIKWLAFAGVLIAVGWSASWVAQDAPFASGGLLGVVSLLLGVTSLLGIPVAVGIA
                     ILRHRLYDIDLVINRTLVYGSLTVLLALVYFGIVTATQAIIQALTGHEQQPQLAIVAS
                     TLVIAALFNPLRRHIQAYIDRRFYRRKYDARKTLEAFSSTLRDETDLEALSDDLVGVV
                     RETIQPAHASLWLRPDTTSNDEQPD"
     gene            6215..6652
                     /locus_tag="VFQ10_02810"
     CDS             6215..6652
                     /locus_tag="VFQ10_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964301.1"
                     /translation="MREMTKDRALVGVVTALALLVLAVGCGLVSDQGRQEAKKKVEAK
                     GQQARQEAKKKVEDLQKKVESKGQQARQEVKKKVEAGQEDLKKKVDDLQKKVEAGQED
                     LKKKVDEVQTKMDDVQKKVTDLQKKVDELLKKVEAQEQQDQKK"
     gene            6757..6966
                     /locus_tag="VFQ10_02815"
     CDS             6757..6966
                     /locus_tag="VFQ10_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HET9964302.1"
                     /translation="MSIARVTELSATSETSFEDAINVAIARATRTLRGVEGAWVKDMN
                     VSIEDGNIRTSRVNLEVTFQLEDPS"
     gene            complement(7009..7425)
                     /locus_tag="VFQ10_02820"
     CDS             complement(7009..7425)
                     /locus_tag="VFQ10_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET9964303.1"
                     /translation="MKTDARNYGIQREYGPRLEAPGPGVMSIEGERTWSVLAHLSTFL
                     NLFTGFLGPIAAFVIWLVYKDDSPTVARHALRSVFYQVAWLSAIFIGWTVTVALMVVL
                     VGFLLVPVMAVVTLAPFVQATYEAYTAYRGDGRIYL"
     gene            7632..8969
                     /locus_tag="VFQ10_02825"
     CDS             7632..8969
                     /locus_tag="VFQ10_02825"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET9964304.1"
                     /translation="MTEESQSAGNGRGTTATGTESLSVTDNRTGKSFDFEISDGTVRA
                     MDFRQIKVDEDDFGLMTYDPGYSNTASCRSAITYIDGEKGILEHRGIPIEQLCEHSSY
                     LEVAYLLIYGRLPTEDELDSWTYEITHHTYVHENIKNFMNGFRHDANPMGMLQASVGA
                     LSTFYPEAKDIDDEYQRHVAAVRLIAKMPTLAAFAYRHSLGLPYVYPDNDLSYAGNFL
                     SMLFKMAEPKYEPDPRLEKALDVLFILHADHEQNCSAAAVRVVGSSRVDPFSAIAAGC
                     AGLYGPLHGGANVAVLRMLQRVGSIENIPAFLEGVKQGDERLMGFGHRVYKNYDPRAR
                     IIRGHVDEVLEATNKESPLLDIAIELEKQALDDDYFTSRKLYPNVDFYSGLIYEALGI
                     PTDAFTVMFAIPRTSGWMAQWMEMIDDPELKIARPRQIYTGPREIDYVPIDER"
     gene            complement(9110..10588)
                     /locus_tag="VFQ10_02830"
     CDS             complement(9110..10588)
                     /locus_tag="VFQ10_02830"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET9964305.1"
                     /translation="MVSREPQDARILSVDVGSSSVRAELYDDAGEGVEGTEVKLDYEF
                     AYTPEGGAEKDADELLDLVVRAVDGTLSEAGGAVISGVAMSTFWHSVLGLDRDGRPTT
                     PILTWADRRAAECVPDLRERLDERAYHRRTGCVPHSSYWPAKLLWASCALPEAFEKTE
                     RWVSPADYFYANFFGEPYQVGTSMASGTGLFDQNRRKWDRETLEVLPAEEAQLSVISD
                     EPRKGLAGEWAERWPPLRETPWLPAVGDGACSNVGSGCTGSDRLALMVGTSGAMRVLW
                     KAESVEIPDGPWCYRADAKRYVMGGALSDGGNLIEWLRNTLRLPDTEKTEEMLSYMEP
                     DSHGLTFLPLLAGERGPGWADRANGTVSGLSMTSKPVEILRAAMEAVAFRFAIIAEML
                     GTASPGEKEVVASGGGLLNSATWTRIMADTLGRPVTLSGVQEASSRGAALIALEALGG
                     PEIEAAQAPLGETFEPDPSRHEVYMKALERQRRLYDAVLGGS"
     gene            10735..11412
                     /locus_tag="VFQ10_02835"
     CDS             10735..11412
                     /locus_tag="VFQ10_02835"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9964306.1"
                     /translation="MASEDRLRELEEKYEDYKVYDNQGERIGKVDDLFVDETDREEYI
                     GVKLGFLGLKSTLIPMDIVRVNESERSIEVSESKDHVRNAPSFDDDEDITPDYEDRIR
                     SYFGLESLGTPESGYGAYTSTGGEHVDSEREEAQAYDTEDNSTDYDYRSSADPEHGER
                     AGESSESGASISSTGSSADEMIREAPATRHTEETESIQEGGRTKIRRRVIREEIIEED
                     DPQSQNS"
     gene            complement(11492..12016)
                     /locus_tag="VFQ10_02840"
     CDS             complement(11492..12016)
                     /locus_tag="VFQ10_02840"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET9964307.1"
                     /translation="MLKCREAKAAGRSVLAMGDCPRLVMFFYGTLKRGERNHERFCGG
                     ALSVEEGAVRGDVYDLPFGYPALVVPGESIHAFGTGDFARDAEEQRRLGRGPVPLLEG
                     PRVFGEIFAFDDPESRLPALDRLEGFDPADASSHYRRVLLPVETNEGPVLLAWAYVVE
                     ESAGSHLPGGKWPP"
     gene            complement(12068..12226)
                     /locus_tag="VFQ10_02845"
     CDS             complement(12068..12226)
                     /locus_tag="VFQ10_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9964308.1"
                     /translation="MAEDAVAAGASVLWMQSGVINEEAAAYAEEHGLIVVMDRCIKVD
                     YATLVGDS"
BASE COUNT         2489 a         3789 c         3844 g         2104 t
ORIGIN      
        1 agcggaccag atggtgcagg gactggggct ggccgagtct acgccaggac ccctgatcat
       61 ggtcacccag tacgtgggct tcatcggtgc ctggaacgat cctggcccct tctcacccct
      121 gctctacggg acgcttgggg cgcttatcac tacctacgcg acgttcttgc cctgctttct
      181 gtttatcttc ttcctggcac cttacataga gcttttggcc aacgaccgca ggctcagggc
      241 gatccttatt ggggttacgg cggccgtggt gggggtggtg gccaacctgg ccgtctactt
      301 cgccacgcag gttctgttcc cggagggcat ctccctggtc ggcctcgaca tcttcgcgct
      361 ggtagtcgcg gtcgtctcgt tcgtggtgtt gcagcgctac aaggtcccga tctacctgat
      421 ggtgccagtg ggcggggtgc tggggatggc gtggaccctg ctgtaagagt agtctgagac
      481 cgtgaagcga ccgcactggg tgctgctagt ctacaggatc ccaagagagc cctcgcgcca
      541 ccgggtggcc gtgtggcgta agctcaggga cctcggtgct ctctacctcc aggatggggt
      601 agcggcgctc cccgaggacg ccgccacccg cgagcagctg gagtggctgc agctgcgggt
      661 gagggaggcc gggggcgagg cgaccctgtg ggaggccaag cccgggacta tggccgagga
      721 agccgagttg gtgggggcct tccgttcctc gagggaggag gcctaccggg ccatcatcgc
      781 gggggcggag cgcataaggc gcaaggcgga gatgggcggc agggccctgt cggagcagct
      841 cggcaagctg gagcgcgagt tcagggcgga gaggcgcagg gactactttc gatccccgct
      901 gcgcctcgag gcagcggggg cactcaaggc cgctcgcgaa gccataaggg accgcgaaag
      961 gtcgcccggg gtcctcggca ccgaggaaaa ggtctgatgt tgtgggccac gcgccgtgga
     1021 tgccacgtgg accgaaccgc ctgcatatgg ttgatccagc gcttcctgga cccgcaggca
     1081 accttcgatt tcttccggga tcccgcggag gccccggagg gagcggagcc cttcgacgtg
     1141 gtcggggcca ggctctccca ccgaggggag gattgctcgt tcgaaacatt cctcaaggag
     1201 tacgagctcg acgaccccgt gctgcgcgag atcgcggaga tagtccacga cgccgacctg
     1261 atggacgaga agtacgggag gcccgagtcc gagggcttgg acgccatcgt gcgcggcatg
     1321 cagctctcac tacccgacga ccacgcgttg atcgaacaca ccgacgccac cttcgacggc
     1381 ctctacgcct acctgcgacg ggagaccctc tagcttgtac cggccaaaga tagaggtccg
     1441 cgggctccgc tacgaggcag gcggcaccga gattctggcc ggcgtcgacg cgctcgtgcc
     1501 ggccggggaa gtcacggccg tcgtggggcc ttcgggggcg gggaagagca ccctgctgcg
     1561 cgcgatcaac cgcctcatag agccgtcctc gggagaggtc tacctcgacg gcgagtcgac
     1621 gggctcgctc gacccgctgg agctgcggcg tcgggtcggg atggtcttcc agatacccgc
     1681 actcttcggg gtttccgtgg aggaggcggt cctgtacggg gcgcgcctgg ccggcaggag
     1741 cgcggatccc gctcggctgc tcgaagtggt ggggctcgac ccttcgatgt gccggcgcgt
     1801 cccggagacg ctctccgtcg ggcagcagca gcgcgtctcg gtggcgcggg ccctcgccct
     1861 ggagcccgag gcccttctgc tcgacgagcc cacgagcgcc ctcgacgagg gcgccaggcg
     1921 caagatagag gagctcgttc gcgatctgaa cgcccgcctc gggctcacga tggtcttcgt
     1981 gagccacgac ctctcccagg tcgagcgcgt cgcagaccgc gtcgtcctgc tggcggaagg
     2041 aaggagcgcg ggacagtggg aaaggccaga gttcttctcg actgcaggcg agcgggcgcg
     2101 gcgactcata gcggggaggc tctagggtgg aggggatgca gacggtcggc tcggcgctgt
     2161 tgacgctcgg gctcgtggcc atcgccgtcg cgctgagcct ctacgagaag ctcggtctgg
     2221 agaaggagat cggcgtcgcc gttttccgct cgttcgtgca gctcgccgcg ataggctacg
     2281 tcatagactt catcttcggg ctcgagagca ttggcgcggt cgcgctcttg ctcgcgggga
     2341 tggtcctctt cgccgcttgg acctcggcga gccgggccgg ggatgtcccg cgggctttcg
     2401 tggtggttgc actcgccgtg ggggtggcgg ccgtggcgac gctcgggttg ctgctcgcgc
     2461 tcggtgtaat accagcgacg gcgcgctacc tgatcccgct cgggggcatg gcgatcggga
     2521 actccatgaa cgccgccagc ctgacgctga cccgcatccg ggacgacgtc agagagcagc
     2581 ggcccaaagt cgaggccgct ctggccctcg gggcaacggg ccgccaagcc gcatccccca
     2641 tcctcaagac ggccctccaa agcgctctca tacccctcat agacgccacg aagacgaccg
     2701 ggatcatctt cctgccgggc gcgatggtcg gcatgatcat cgccggcgcg aaccctttgg
     2761 aggccgtgcg gcttcagatc gtcgtcctct acatgctctt ggggagcgtc tccatagccg
     2821 ccattctggt cggcacgctc tcttaccgct cgttctttac acccaggcac cagatccgaa
     2881 aggcgatgtc ggacgagtag tttgaccgcc cagtatcatg gaggggcgcc tatcctacac
     2941 tcgcctcgct gccgtcagat gccagcatcc ccatcgcctc gacatggatc tcgccccgca
     3001 cgcggaccag cgtcagcata cccgggactg tgctgcgtgc gacatggctg gccatgacct
     3061 cgtgttgcgg gccagccgcg cctcggacgt tccccgtcac tcggccactg gcatcggcgc
     3121 gtcgaggaac ggagggatta tcgaaagcag cagatcggcg cggtgcacaa ccgtcacgtg
     3181 agaagtgccg gggagcaccg cgagttgtga attcgggagg ccggcggggg tatcgccgaa
     3241 gacgccgccg ccgagcagtc gaaacatctc aacagcgtgc tcgggacgga cgagatcgga
     3301 atcgccgata atgagtaggg tgggcgcctt gatcgactga acgacctcgg ccggaacgtc
     3361 cttgatgttc cgatccatct gcgtcttttt ggcgaacagc gtggcgaaat tctccggatt
     3421 cggcgctatc cgagtgtatt cctcgtgcca cgaagaccca aacatcattt cgggtttcat
     3481 gtccgctaat ccttccatga gcccggggtg gacgccgctc aggttgtaag tgaccgaggc
     3541 gagcacgaga ttgcgcacca cgtccggatg tcggatggca agatgcaacg cgacgctggc
     3601 gcccatgctg tagccgaaaa tatcggcctg agcgatcccg aggtgacgga gcgctgcggc
     3661 ggtgtcatcg gccatttgct cgatgctcag cgggcgatcg atgtcggccg tgcgcccgtg
     3721 tccctgcagc tcgaaggcga tgacctgccg agtctcggca agagccggca gaagctctcc
     3781 gaacgacgtg ccgattgccg aaaatgcgcc gtgaagcaga accagcggcg gtccggagcc
     3841 gtggatctcg taatacatct cgagaccgtt cactgaagcg tagcggccag cctccggggc
     3901 ccgagtatcg tcgccgttcg tcatgatggg ttgctcctat cttggcggtt ggcattgtct
     3961 ggtatcagca tacggcaata atctcgaaca accagagaat tctacagagc aaacaagaaa
     4021 acacccgggt gaataggcct accctcgagc ttactgctac cgaatgcggg ggttggacgg
     4081 aagctatact ggatacgaac tttgaagaat ttgcctttta cgatattggg tgaatgactt
     4141 gtctaaggtg ggttgtcgat agcagtgcga gaataggcgt gcaaacaaag ggacagctga
     4201 ggcgatagga gtccgagccg atgaagcaat tacacactca gatcgagatc gacgcccccg
     4261 ctaagcgcgt gtgggaattg ctcacagact tcgcctcata ccctcagtgg aacccattca
     4321 tccgccacat cagcggccga cttgctcccg gggagcgcct ccaagcacgt ctcgagcctc
     4381 ccggcgggcg agccatgacc ttcaagccca aggtcttagc ggctgaacct gaccgcgagc
     4441 tgcggtggct aggccaccta ttggtgcctg gtgtgttcga tggtgagcac tccttcacca
     4501 tcgagccgct agaagagaac cgtgttcgtt tcgtccagcg agaagcgttc aagggtctgc
     4561 tcgtacctct tttcgcccgg agtctcgaga ccaacacgca gcgggggttt gaggagatga
     4621 accgcgccct caaggagcgt gccgagaccg cccctgcgca agactaacct tcttcttgac
     4681 ccagcgcctc tgcacaaggc agcggctagt ctggctgctc gtcgttcgag gtcgtatcgg
     4741 gacgcagcca cagcgaggcg tgggctggct gtatcgtctc ccttaccacc cccaccaggt
     4801 cgtcgctcaa tgcctccaga tctgtttcgt ccctgagcgt cgacgagaag gcctctaagg
     4861 tctttctagc atcgtacttc cttctataga agcgcctatc tatgtacgcc tggatatgcc
     4921 tacgcagggg gttgaacagg gctgctatca caagggtcga tgccactatg gccagctgcg
     4981 gctgctgctc atggccggtg agcgcttgga tgatggcctg ggtcgccgtc acgattccga
     5041 agtacacgag cgccagcaag accgtgagcg agccgtagac gagggtgcgg tttatgacga
     5101 ggtctatgtc gtagaggcgg tgcctcaaga tcgctatacc cacggcgacg ggtatgccga
     5161 ggagggaagt gaccccaagc agcaaggaaa ccaccccaag cagtcccccg ctagcgaatg
     5221 gggcgtcctg agccacccaa ctggctgacc aaccgaccgc tatgagaaca ccggcgaaag
     5281 cgagccactt gatctgctgc cgttcctctc ctgtcgcccc gcgatatcgc accgctatgg
     5341 agaccgctga gagcaccacg ctcgccgaga gcagacccca cccgaagcca ccgtcttcca
     5401 ggacgctgcc ttcgagcggg gcgagcccca cagggttgtc gatctccggg tagtcgctca
     5461 gaggccctgg cgtgaaggcc aagccgcagg ctagcgtcac cagggccacc acggagagcc
     5521 agcccacgac cctccagcgg cgtgagggaa gccgcccgtc agggaacatc aggagcccga
     5581 acgtcatggc gggacccacc ccaggtaccc agatccagta ggcgaaccag gcggcccact
     5641 ccgtagccgg caaaccgctc ggccctgtga gcgaaaacac ggcgtaaccg ctggcgaggg
     5701 tcgtagcccc gatggtcaga cctgccgcac acaagatcca gccgacgccg ttgccgggtc
     5761 ggcgtgccac gatgagagcg ccgaccaaag agaacgccag caccagggcc gccaaggcca
     5821 ggacggcgac aatctggggg acgccgagga gcacgaccga ggctgaagtg gaccagtaca
     5881 ggggcacgga caggaggacg aacgccgcgg agagcgccca cgctgacccg gccaatagtg
     5941 cggagatccg agggctcatc tcgaccggct catagctcgg tgccgatgtc tcccttgcac
     6001 gcgcggtgac atacctcctt actctacacg gtgatcgtcc ccaaaacgtg acggcaaccg
     6061 ttatgaggga aagttagaga ggaaagatca gagcatccta ccatcggcgt ttggcgatct
     6121 ccgcagagat caataacacg cacagaagac ttggagatcg taagatccta aagcaaaaaa
     6181 ggacacagca accgaaagga caagggagag gacgatgcga gaaatgacga aggatcgggc
     6241 gttggtgggg gtagtgactg cgctagcgct actggtctta gcagtgggat gcggtctggt
     6301 ttccgaccag ggccgccagg aggcgaagaa gaaggtcgag gccaaaggtc agcaggccag
     6361 acaggaggcg aagaagaagg ttgaggacct ccagaagaaa gtggagtcca aaggtcagca
     6421 agccaggcag gaagtgaaga agaaagtgga ggctggccag gaggacctta agaagaaggt
     6481 agacgatctc cagaagaagg tggaggccgg ccaagaggac ctgaagaaga aggtggacga
     6541 ggtgcagacg aaaatggacg acgtgcagaa gaaggtaacc gacctccaga agaaggtgga
     6601 cgagttgctg aagaaggtag aggcccaaga gcagcaggac cagaagaagt aaacgaagca
     6661 gccgtccgac tcatccggag gtgaggatga cgaggattac cagaggacca tccacgagca
     6721 ttaagcggca agcttggggt tagggaggcg agtaccatga gcattgcccg agtcaccgag
     6781 ttgagcgcca catccgagac gagcttcgag gacgccatca acgttgccat cgcccgcgcc
     6841 accaggacgc ttaggggcgt agagggcgcg tgggtcaagg atatgaacgt cagcatagaa
     6901 gacggcaaca tcaggacctc cagggtcaac ctggaggtaa ccttccagct ggaagatccc
     6961 tcctagagcg gataccacac ctgctgtttg tagactgaaa cctggtgcct acaagtagat
     7021 gcgtccatcg cctcggtagg ccgtgtacgc ctcgtaggtg gcctgtacga aaggtgcgag
     7081 cgtcacaacg gccatgacgg gcacgaggag gaacccgaca aggacgacca tcagcgcgac
     7141 cgtcacggtc cacccaatga agatggcgct gagccaggca acctggtaga agacagatct
     7201 gagcgcgtgc ctggccaccg ttggcgagtc gtctttgtag acaagccaga tcacgaacgc
     7261 cgcaataggc ccgagaaagc ccgtaaagag gttcaagaac gtgctcaaat gggccagcac
     7321 cgaccacgtg cgctcgccct caatgctcat cacgccagga ccaggtgcct cgagccgcgg
     7381 gccgtactcg cgctggatcc cgtaattcct cgcgtcagtc ttcatgtgcg ctccttcctc
     7441 accttggcta accgcgtata cgcgggcgca agacgaaggt ttcgtccccc atgtggccgc
     7501 ttcgcgcaca tcactttccg tttcccctcc agtgcgtgat aatacccgta cctcatagtg
     7561 cgttttggcg cggacacata tatggcacga gcatatataa ttaacgtgcg tgcaacagaa
     7621 aggggcacag gttgaccgaa gagtcgcaga gcgcgggtaa cgggcggggt acgactgcca
     7681 cggggaccga gagtctatcg gtcacggaca atcgtacggg gaagagcttt gacttcgaga
     7741 taagcgacgg cacggttcgg gccatggact tcaggcagat caaggtcgac gaagacgact
     7801 tcggcctcat gacctacgac cctggttact ccaacacggc gagttgcagg agcgccatca
     7861 cctacataga cggggagaag gggattctgg agcatcgtgg catccccatc gagcagctat
     7921 gcgagcactc ttcctacctc gaggtagcct atctactaat ctacggcagg ctccctaccg
     7981 aggacgagct ggatagttgg acctacgaga taacccatca cacctacgtt cacgagaaca
     8041 tcaagaactt catgaacggc ttccgtcacg acgcgaaccc gatggggatg ctgcaggctt
     8101 ccgtcggggc cctctcgacc ttctaccctg aggccaagga catcgacgac gagtatcagc
     8161 ggcatgttgc ggcggtgcgt cttatcgcca agatgccgac tctcgccgct ttcgcctacc
     8221 gtcatagtct cgggttgccc tacgtctacc ccgacaacga cctctcctac gcgggcaact
     8281 tcctgtccat gctcttcaag atggccgagc ccaagtacga gcccgacccg cgcctggaga
     8341 aggcgctcga cgtgctcttc atactgcacg ctgaccacga gcagaactgt tcagccgccg
     8401 ccgtaagggt cgtggggtct tcgagggtcg accccttctc ggccatcgcc gccggctgcg
     8461 ccgggctcta cggaccgttg catggcgggg ccaacgtcgc tgtcctcagg atgctccagc
     8521 gcgtaggaag tatcgagaac atccccgcct tcctcgaagg cgtcaagcaa ggtgacgagc
     8581 gtctaatggg cttcggccac agggtgtaca agaactacga cccgcgggcc aggatcatcc
     8641 gcggccacgt cgacgaggtg ctcgaggcca cgaacaagga gagtccgctg ctcgacatcg
     8701 ccatcgagct cgaaaagcag gcgctggacg acgactactt cacgagccgc aagctctatc
     8761 ccaacgtcga cttctactcg ggcctcatct acgaagccct cgggataccg acggacgcct
     8821 tcaccgtcat gttcgccata ccacgcacct cgggttggat ggcccagtgg atggagatga
     8881 tcgacgaccc cgagctcaag atcgcccgtc cgcgccagat ctataccggc ccccgcgaaa
     8941 tagattacgt gccaatagac gagcgctaac gcgcccgtca gcacttcagc acgctctggc
     9001 ctgcggcctc cgctttcagc aagtcagcca gggcttttgc ccacgcgaag gctgaaatgc
     9061 tgaaagcgcc cgacagggcg cgtgctgaag tgctgacatg ctggaggctc tatgagcctc
     9121 ctagcacggc gtcgtaaagc cggcgctggc gttcgagtgc cttcatgtag acctcgtgcc
     9181 tggatgggtc tggttcgaag gtctcgccga gtggcgcttg tgcggcctcg atctccggcc
     9241 ctcccagcgc ttccagggcg atcagggccg cgcccctgct ggaagcctcc tgtacacccg
     9301 agagcgtgac cggtctgccc agagtgtcgg ccatgatcct ggtccacgtc gctgagttga
     9361 gcaggccacc gccgctggcg acgacctcct tctcgccggg ggaagcagtt ccgagcatct
     9421 ccgcgataat ggcgaacctg aaggccacgg cttccatcgc ggcacgcagg atctctactg
     9481 gcttgctagt catcgagagc ccggagacgg ttccattggc cctgtcggcc catcccggtc
     9541 cacgctctcc ggcgagcagg ggcaggaacg tgaggccgtg ggagtccggc tccatgtagg
     9601 acagcatttc ctcagtcttt tcggtgtcgg ggagacgtag cgtgttgcgc agccattcga
     9661 tcaggtttcc gccatctgag agcgcgccgc ccatgacgta gcgtttcgcg tcagccctgt
     9721 aacaccaggg accatcgggt atctcgacgg actccgcttt ccacagcacc cgcatagcgc
     9781 cgctagtgcc gaccatcagc gccagccggt cactacccgt gcacccgctc ccgacgttgg
     9841 agcaggcgcc gtcgcccacc gcgggtagcc agggcgtctc tcgcaggggc ggccaccgct
     9901 ctgcccactc cccggccaac cccttacggg gctcatccga gatgacggag agctgggcct
     9961 cttcggcggg caggacctcc agtgtctcgc ggtcccactt ccgccggttc tggtcgaaga
    10021 ggcctgttcc cgaggccatt gacgtaccca cctgatacgg ctccccaaag aaattggcgt
    10081 agaagtagtc ggcaggggag acccagcgtt ccgtcttctc gaaggcctcg ggcaaggcgc
    10141 agctcgccca caacagcttg gcaggccagt agctggagtg gggcacgcaa cccgtgcgac
    10201 ggtggtaggc cctctcatcc aggcgttcgc gcaaatctgg tacgcactca gccgcgcggc
    10261 ggtcggccca ggtaaggatc ggggtcgtag gacgcccgtc ccggtcaagg ccgaggaccg
    10321 agtgccagaa ggtgctcatg gcgacaccgg agatcaccgc tccaccggcc tcggagagtg
    10381 tgccgtctac ggcgcggacc acgaggtcga gtagttcgtc ggcgtctttc tcagcgccgc
    10441 cttccggcgt gtacgcgaac tcgtagtcga gcttcacctc ggtgccctct acgccttcgc
    10501 cagcgtcgtc gtagagctcg gccctcaccg aagaggaacc aacgtccaca gagagaatcc
    10561 tagcgtcctg aggttccctg gataccacgc tcccacctcc ctgaaagatc attcttcatg
    10621 gtagtttagc aagccgcttc ccctcatcat tctccaggag cgcgcatgtt taccggcagc
    10681 cctgactcgg gtaacgagat cacaaacagc cgcaggcttt tctaggagga gaacgtggcg
    10741 agcgaagata ggttgaggga gctcgaggag aagtacgagg actacaaggt ttacgacaat
    10801 cagggggaga ggatcggcaa agtcgacgac ctcttcgtgg acgagacaga ccgcgaagaa
    10861 tacatcggcg tcaagttggg gttcctcggc ctcaagtcca ccctgatccc gatggatatc
    10921 gtccgcgtaa acgagagcga aagaagcata gaagtctcgg agtcgaagga ccacgtaaga
    10981 aacgccccga gcttcgacga cgacgaggac atcacccctg actacgagga tcgtatccgt
    11041 agctacttcg gcctcgaatc cctgggaacc cccgaaagtg gctacggtgc ttacacgagc
    11101 accggcggcg agcatgtgga cagcgagcgc gaagaggcgc aagcctacga cacagaggac
    11161 aattctacgg actacgacta ccgttcttcc gcagaccccg agcatgggga acgcgcggga
    11221 gagtcctctg agagtggagc gtccatcagc agcacagggt cctccgctga tgagatgatt
    11281 cgggaggcgc cggccacgcg ccacaccgaa gagaccgaga gcatacagga gggtggacgc
    11341 acgaagatcc gccgccgtgt gatacgcgag gagatcatcg aagaagacga cccccagagc
    11401 cagaacagtt agctaacgct cccagctcat cagcaaaaca ggaacctgac accgcgacat
    11461 gcgcaaggtc atcgacctcg tgcatactgg attacggtgg ccatttgcct ccgggcaggt
    11521 ggcttcctgc tgattcttcg actacgtagg cccacgccag gaggaccggg ccctcgtttg
    11581 tctcgacagg gagcaatacg cgccggtagt ggctcgacgc atcggccgga tcaaagcctt
    11641 ccagacggtc gagggctggt agacgcgact ccggatcgtc gaaagcaaag atctcaccga
    11701 agacgcgtgg accctccagc aatggcaccg gcccacggcc gagccgccgt tgctcctcag
    11761 cgtcccttgc aaaatccccg gtcccgaaag catgaatgga ctctccaggg acgaccagcg
    11821 caggatagcc aaagggaaga tcgtacacat ccccacgcac cgccccctct tcgacgctca
    11881 gggcaccgcc gcagaaacgc tcatggttgc gctcaccgcg tttgagcgta ccgtagaaga
    11941 acataaccag ccgcggacaa tcacccatag caagcacgct tcggcctgcg gccttcgctt
    12001 ctctgcactt cagcacggcg gcgaagccgc cttttcagca agtcagcatc gcgcgctgac
    12061 atgctgacta actgtcacct acaagcgtcg cgtaatctac ctttatacag cggtccatga
    12121 cgacgatgag gccgtgttct tcggcgtagg cggcggcctc ttcgttgata acgcctgatt
    12181 gcatccacag aacgctcgcg ccggcggcca cggcgtcttc ggccac
//
LOCUS       DASTKM010000073         4893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_896632, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000073 DASTKM010000000
VERSION     DASTKM010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896632"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFQ10_02850"
     CDS             <1..618
                     /locus_tag="VFQ10_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET9964309.1"
                     /translation="VASKRKVSNPLALAVMALLYERPMHPYEMVTLMRERGKHESVRL
                     RYSSLYSVVGALEREGLIVPRETVREGRRPERTVYEITGAGREEFLTWLRELLREPVK
                     EYTQFAAGLSFLAGLPPEEAVALLKERVRRLERENEEMRSRLATAMEQWNLPRLFLVE
                     SEHELMLREAELGWVREIVEEIEAGRLGSLSEWRALHEGHGEGGE"
     gene            618..1064
                     /locus_tag="VFQ10_02855"
     CDS             618..1064
                     /locus_tag="VFQ10_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964310.1"
                     /translation="MSERAGLPRWLKLANPVIVALQRRGVVIGTMRLLAVPGRKSGQL
                     RTTPVSPLMVEGERYIIAGLEEADWVKNARVAGWGILARGREQERVNLMELPLQERAP
                     VLREFPNRVPHGVQFFRQLYGISGTPQEFEALASRCPVFRVEEAGP"
     gene            1262..1771
                     /locus_tag="VFQ10_02860"
     CDS             1262..1771
                     /locus_tag="VFQ10_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HET9964311.1"
                     /translation="MTDQTTRTIGARHADEAAIRELFGLLLDDWGRGDGEAYGSRFTE
                     DADYVAFDGTRTRGRREISASHQRLFDKFLKGTRLTGRVLSIKFPSPDVALVHATGGT
                     VMRGKTRPSPERDSIQTLVAVREGAEWRFAAFHNSRVRPIGSNAATFLLWAVSDRLWR
                     TFAPGKEDA"
     gene            1768..2970
                     /locus_tag="VFQ10_02865"
     CDS             1768..2970
                     /locus_tag="VFQ10_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9964312.1"
                     /translation="MRRAKRALIIGGGVAGPVAAMALRQAGIDSVVYEAYAGGADGAG
                     GFLTFASNGMDALHAIDAHHLVLAEGFPTPRMTIQNGTGKHLGEVPLGGKLPDGTVSQ
                     TLKRADLYRTLRDEAVRRGARVEYGKRLVDAETTPDGSVTARFEDGTEAVGDLLIGAD
                     GIHSRTRRIIDTSAPGARYIPVLNIGGYARDVRVLAEPGTFRMVFGKRAFFGYAVHPS
                     GEVWWFANPPRADEPTRAELAAIGTEQWREMLMDLFAGDATPAVEIIRSTPGELAGWA
                     TYDLPSVPTWHKGSLVIIGDAAHATAPSSGQGASMAIEDAVVLARCLRDLPDIGQAFA
                     AYERLRRTRVERIVAHGARTSNSKAAGPVARVLRDMMMPLILKRVASGESLAWMHDYH
                     IAWDEKVA"
     gene            complement(3018..3485)
                     /locus_tag="VFQ10_02870"
     CDS             complement(3018..3485)
                     /locus_tag="VFQ10_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9964313.1"
                     /translation="MGKGVNPIPEGFHTVTPYMTIKDASDAIEFYKEAFGATEIFRWA
                     DPDGRVRHAEVVIGDSPIMLTDEAPEFGMAGPQSLGGSPVHVFLYVEDADAVFERAIE
                     AGATELMPVEDSEDGDRRGGVTDPFGHVWYIGTHVEDISREELQKRYDAMVNQ"
     gene            complement(3602..4846)
                     /locus_tag="VFQ10_02875"
     CDS             complement(3602..4846)
                     /locus_tag="VFQ10_02875"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET9964314.1"
                     /translation="MGPVEFSVETARELLLAAQGLGSVPERATKDDVRGIIRRMGVLQ
                     IDSISVVARSPYLVLWSRLGSYEPRWLDELLAEGVLFEYWSHAACFIPIEDYGLYRRL
                     MIEGGEKTRSWMQAHHEEIEHVMERVSENGPVRVAEFARTDGKAGGWWEWKPEKRALE
                     HLFAAGELMISRRENFHRVYDLRERVLAKALPDWEDALAPNEQEVRRALALKTVRALG
                     VALARWVPDYFRTPKKGVSGLLEELADEGSLLRARIEDEPAYLHPDNAGLAEEILSGG
                     LRSSVTTLLSPFDPVVWDRARALELFGFEYKIEVYTPAARRRYGYYSLPILHSGSLVG
                     RLDAKAHRKQGTFEVKSIHLEPDMPVNDDLVSELAYALRDCAGWHGTPEVLVRRSDPT
                     ELTEPLRLALDGGAVPRSAKTT"
BASE COUNT          891 a         1575 c         1579 g          848 t
ORIGIN      
        1 gtggcgtcga agaggaaggt atccaacccg ctggctctgg cggtgatggc gctcctctac
       61 gagcggccga tgcaccctta tgagatggtc acgttgatgc gcgagcgggg caagcacgag
      121 tcggtgcggc tgaggtacag ctctctttac tcggtagttg gtgctttgga gcgggagggc
      181 ctcatcgtgc cgagggagac ggtacgagag gggcggcgtc ccgagcggac ggtctacgag
      241 atcacgggcg cgggacgcga ggaattcctc acctggctgc gcgagctttt gagagagccg
      301 gtcaaagagt acacgcagtt tgcggccggg ctttcgtttt tggccgggct tcctcccgag
      361 gaggctgttg cgcttctgaa ggagcgggtg cggcgactgg aaagagagaa tgaggagatg
      421 agatcccgtc tggccaccgc catggagcag tggaacctac cacgcctatt cctcgtagag
      481 tcggagcacg agttgatgtt gcgggaggcc gaactgggat gggtgcgcga gatagtagag
      541 gagatcgaag ccggaaggct gggcagtctg tcggagtggc gcgccctcca tgaggggcat
      601 ggcgagggtg gagagtgatg tctgagagag ccggactacc ccgatggctc aagctcgcca
      661 atcctgtgat cgtggccttg cagcgccgcg gggtagtgat cggcaccatg cggctgctgg
      721 cagtgcctgg ccgcaagagt ggccagctgc gtacgactcc ggtttcaccg ctgatggtgg
      781 aaggcgaacg ctacatcatc gccggcctcg aagaagcgga ctgggtcaag aacgcgcggg
      841 ttgcggggtg ggggatcctg gcgcgaggcc gagagcaaga gcgggtaaac cttatggagt
      901 tgccgctcca ggagcgcgcc cccgtcttgc gtgagttccc gaacagggtg ccacatggag
      961 tccagttttt tcggcaactc tacggtatct ccggcactcc acaagagttc gaagcgctgg
     1021 cgtcccgatg tccggtgttc cgggtggagg aggctgggcc gtgatctgaa taaagaggac
     1081 ccccggcggg gtgctgcaac acctcatgcc ggaggtcgta atccccgagt agacaggacc
     1141 gcgcagggcg agacccgtta cacgaggcgt gtatttccaa ggataacagg tcgaagcgct
     1201 ccgcggaacg cagccaggga tctctgaccc gatccctttt gacgaaggaa ggtattcgac
     1261 aatgaccgac caaaccaccc gcacaatcgg agcgcggcac gcggacgagg cggccatcag
     1321 ggagctgttc ggtttgctcc tcgatgactg gggcagggga gacggcgagg cttacggttc
     1381 ccgtttcacc gaggacgccg actatgtagc tttcgacggg acgcgcacca ggggacgccg
     1441 ggagatctcc gcatcacacc aacgactctt cgacaagttc ctcaagggca cacgcctcac
     1501 gggccgcgtc cttagcatca aattcccgag ccctgacgtt gccctcgtcc atgcgaccgg
     1561 aggcacggtt atgcgcggca agacgaggcc ttcgccggaa cgggattcga tccagaccct
     1621 cgtcgccgtg cgcgagggcg ccgaatggcg ctttgccgcg ttccataaca gccgcgttcg
     1681 tcccataggc agcaacgcgg ccacgttcct actctgggct gtcagcgaca ggctctggcg
     1741 taccttcgcg ccgggaaagg aggacgcatg agacgcgcga agagggccct gatcatcggc
     1801 ggcggcgtcg ccggtccggt ggccgcgatg gcgctaaggc aagctggaat agactctgtc
     1861 gtctacgagg cgtatgcggg cggcgcggac ggcgccggcg gcttcctcac cttcgccagc
     1921 aacggcatgg acgccctgca cgccatagac gcccaccacc tcgtgctggc cgagggcttc
     1981 cccacgccgc gcatgacgat ccagaacggt accggcaagc acctcggcga ggtccccctc
     2041 ggcgggaagc tgcccgatgg cacggtcagc cagaccctca agcgggccga cctctaccgg
     2101 acactgcgcg acgaggcggt caggcgcggc gctcgcgtcg agtacggcaa gcggctcgta
     2161 gatgccgaga ctacgcctga cggcagcgta acggcgcggt tcgaggacgg taccgaggcc
     2221 gtgggagacc tcctgatcgg ggccgacggc atccactccc gcacccgccg catcatcgat
     2281 acgtcggcgc ccggggcccg ctacatcccg gtgctcaaca tcggaggata cgcccgcgac
     2341 gtgcgtgtcc tggccgaacc tggcacgttc cggatggtct ttggcaagcg ggcgttcttc
     2401 ggctacgcgg ttcatccctc aggcgaggtc tggtggttcg ccaaccctcc ccgggccgac
     2461 gagccgacaa gggcggagct cgccgccatc ggaaccgagc agtggaggga gatgctgatg
     2521 gacctcttcg ccggagacgc cacaccggcc gtcgagatca tccgttccac ccccggagaa
     2581 ctcgccgggt gggctaccta cgacctcccc tccgtcccaa cctggcataa gggttcgctg
     2641 gtcatcatcg gggacgcggc gcacgccact gctccttcct cgggacaggg cgcgtctatg
     2701 gccatagagg acgccgtcgt gctcgccagg tgcctgcgcg acctgcccga tataggacaa
     2761 gccttcgccg cctacgagcg gctccgcagg accagggtgg aacgcatcgt cgcgcatggg
     2821 gcgaggacga gcaacagcaa agccgcaggc cccgtcgccc gggtgctgcg cgacatgatg
     2881 atgcccctga tcctcaagcg cgttgctagc ggtgaatccc tcgcatggat gcacgactac
     2941 cacatcgcct gggacgagaa ggtcgcgtaa ctagagctcc ggccgcttag tgcgggtggt
     3001 caaggcgatg cttgcggcta ttggttcacc atagcatcgt accgtttctg gagttcctcg
     3061 cgggagatgt cctcgacgtg cgtgccgata taccagacgt gcccgaaggg gtccgtcacc
     3121 ccgccgcggc ggtcgccatc ttcgctgtcc tcgaccggca tcagctccgt cgcccctgcc
     3181 tcgatcgccc tctcgaacac cgcgtccgcg tcctctacgt agaggaacac gtgtactggc
     3241 gagccgccta gcgattgcgg gccggccata ccgaattcgg gcgcctcatc ggtgagcata
     3301 atcggcgagt cgccgatcac gacctccgcg tgccggacgc gcccgtccgg gtccgcccag
     3361 cgaaaaatct ccgtcgcccc gaacgcctcc ttgtagaact cgatggcgtc tgacgcgtct
     3421 ttgatcgtca tgtacggcgt aacggtgtga aaaccctccg ggatgggatt gactcctttg
     3481 cccattgtcc tcctttgcaa gctcacgcct tcaaacacaa tggaagtgta cgcacaaacc
     3541 acctggttcc catcctcgga ttccgccggt gcggacgacg attttgccga cttcgtcgtg
     3601 gctaggttgt cttcgcggat ctcggtacgg caccgccatc gagcgcgagc cggagcggct
     3661 ctgtgagttc cgtaggatcc gaccggcgga ccagcacttc gggcgttcca tgccagccgg
     3721 cgcaatcacg cagagcgtag gcgagctcgg agacgaggtc gtcgttcacc ggcatgtccg
     3781 gctccaggtg gatcgacttg acctcgaaag ttccctgctt acggtgggcc ttggcgtcga
     3841 gcctgcccac gagggagccg ctgtggagta tcgggaggga gtagtatccg tacctgcggc
     3901 gggccgccgg agtgtagacc tcgatcttgt actcgaagcc gaagagctcc agagcccgcg
     3961 cccgatccca gaccacagga tcgaacgggg acagcaaggt cgtgacagaa gaccgcaacc
     4021 cgcccgatag gatctcctcg gccagcccag cgttatcggg gtgcaagtag gcaggctcgt
     4081 cctcgatcct ggcccgaagg agtgagcctt cgtcggccag ctcttcgagt agtccggaga
     4141 cacccttctt gggtgtgcgg aagtagtcag gaacccagcg ggccaacgca acgccgagcg
     4201 cgcgtaccgt cttgagagcc agcgcccgtc gcacctcttg ttcgttcggc gcgagggcgt
     4261 cttcccaatc tggcagcgct ttagcgagca cgcgctcgcg cagatcgtag acgcggtgga
     4321 agttctcgcg acgggagatc atgagctctc ctgcggcgaa caggtgctcc agtgcccgct
     4381 tctcgggctt ccactcccac cagcctcctg ccttcccgtc ggtgcgggcg aactccgcga
     4441 cgcgcacggg accattctcg ctgacgcgct ccatcacgtg ctcgatctcc tcgtgatgag
     4501 cctgcatcca gctcctggtc ttctcccctc cctctatcat gagccgccgg tacagaccgt
     4561 agtcctcgat cgggatgaag cacgccgcgt gcgaccagta ctcgaacagc accccctcag
     4621 ccagaagctc gtccagccag cgcggctcgt aggaacctag cctgctccac aacaccaggt
     4681 agggactgcg cgcgacgacg gagatcgagt cgatctgcaa cacccccatc cgccggatga
     4741 tcccccgaac atcgtccttc gtggcacgct ccggcacgga acccaacccc tgcgccgcga
     4801 gtaacaactc ccgcgccgtc tctacagaaa actctacggg tcccacctcc tgtcgacaac
     4861 cgtatagtta tacggcagaa gtctgagtgt aac
//
LOCUS       DASTKM010000074         2487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1249761, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000074 DASTKM010000000
VERSION     DASTKM010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249761"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFQ10_02880"
     CDS             <1..208
                     /locus_tag="VFQ10_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964315.1"
                     /translation="AVGELGTSETERQIAAAEIDIKRLDAERELEMMEVQMGLREPEP
                     IEAAPTEETAKPQPEETEEGPRGQ"
     gene            198..989
                     /locus_tag="VFQ10_02885"
     CDS             198..989
                     /locus_tag="VFQ10_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964316.1"
                     /translation="MDSRKNLSRRLMLRLAPFATFAGLGTFAALESSYILAGLLGIGA
                     FVTGRRLLDRRENRRKELLSRARHNRGELGRVAREDRIAASQMKRLVALQDGLLESWE
                     LLPEECRPLLDEDIFTIIGEVEDAARLARRRAALRRHLESVDRHSIHGRIKELQKDLS
                     HLEPGSPMRTSFESALQGRRGELAGYDDVLGGISMINAQLESAESLLGNLRGELLALD
                     DSLVSGPQDPGLARLKERIAYFRHGLDEVTRRVETLPATERMSAR"
     gene            986..1645
                     /locus_tag="VFQ10_02890"
     CDS             986..1645
                     /locus_tag="VFQ10_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964317.1"
                     /translation="MNEDRNTSRQSFFSRVFAGSTEGEETSIYASEEIGMPGTDESEG
                     LAAHGFTVERAAEVIRNLPPEVPRPAAVRIVRGTLEAAGIDIAELETSTRARESKLNS
                     EIDLSENRIQRFKDETEEVIRNLEDEIRKAREARDFGVSEEERKISSAEAGLDNIDIV
                     RDFFGLPQEEADEPPAAGEETQVMEGVEDEEEDDTQVLRRPGPLSDSEDYLEPGDKKE
                     Q"
     gene            1642..2100
                     /locus_tag="VFQ10_02895"
     CDS             1642..2100
                     /locus_tag="VFQ10_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9964318.1"
                     /translation="MSIPRRLGRLAQGFVSSLQEDERFRERLRTGRERSEALRGAFEA
                     AWKGASEEWRKAEERRAAQEAAAEQTWGWRSATTTFSPRKYPPNVLAAYDRLGLVPSA
                     SMEEVDRKRRELVKRFHPDRFSDPEKRLRAERVTAEINAAHDAIERHVAG"
     gene            complement(2184..>2487)
                     /locus_tag="VFQ10_02900"
     CDS             complement(2184..>2487)
                     /locus_tag="VFQ10_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HET9964319.1"
                     /translation="GLPWIAGSNTFITGHALGFPRTRVPYVFYKLKKMRPGDEILIED
                     KEGKKYTFRVYDFMTVEPNDYWAIYPVEGKTVVSLQSCTPIPTFENRLIVRGELVS"
BASE COUNT          539 a          723 c          831 g          394 t
ORIGIN      
        1 ggccgtaggc gagctcggca ccagtgagac cgagcgccag atagcggcgg ccgagatcga
       61 cataaagcgg ctcgacgccg agcgggagct cgagatgatg gaggtccaga tgggcctgcg
      121 cgagccagaa ccgatcgagg ctgcgccaac cgaggagaca gcgaagcctc agcccgagga
      181 gaccgaggag ggaccgcgtg gacagtagaa agaacctctc gcggcggctg atgctacgct
      241 tggccccttt cgctaccttc gcaggtctcg gaacctttgc ggctctcgaa tcctcttaca
      301 tcctcgccgg cctgctaggc atcggggcct ttgttacagg ccgtcggctc ctcgacaggc
      361 gagagaatag gcgcaaggag cttttgagcc gggccaggca caacagggga gagcttggcc
      421 gagtggcgag ggaggatcgc atcgcggcgt cgcagatgaa gcggcttgtc gccctgcagg
      481 acgggctgct ggagagttgg gagcttctgc ccgaggagtg caggccgctt ctggacgagg
      541 atatcttcac catcatcggc gaagtcgagg acgcagcgcg actggcgcga cgtcgggcgg
      601 cactgaggag gcatctggag agcgtggacc ggcattctat ccacggtcgc ataaaggaac
      661 tgcagaagga tctctcgcat ctggagccag ggtcgcccat gcggacgtcg ttcgagagcg
      721 cgctccaggg acgccgcggg gagctcgcag gctacgacga tgtgctcggc ggcatcagca
      781 tgatcaacgc ccagctcgag agtgcagaga gcctgctcgg gaatctgcgt ggagagttgc
      841 tggccctgga cgacagtctc gtctctgggc cgcaagaccc tggcctcgcg cggctcaaag
      901 agcggatcgc ctacttccgc cacggactag atgaggtgac gaggcgcgtc gagaccctac
      961 ccgctacgga aaggatgtcg gcgagatgaa cgaggatcgc aacacgtcgc ggcagagctt
     1021 cttctccagg gtgttcgcgg ggagcaccga gggcgaggag acctctatct acgcctcgga
     1081 ggagatcggt atgccgggga cagacgagtc ggaaggtttg gcggcgcacg gattcaccgt
     1141 cgagcgggcc gccgaggtca tcaggaacct ccctcctgag gtgccgagac ctgccgccgt
     1201 gcgcatcgta cgtgggacgc tcgaggcggc gggtatagac atcgcggagc tggagacctc
     1261 cacgagggcc agggagtcca agctcaactc agagatagac ttgagcgaga atcgtataca
     1321 gcggttcaag gacgagaccg aagaggtgat ccggaacctc gaagacgaga tacgcaaggc
     1381 gcgcgaagcc agagacttcg gcgtctccga agaagagagg aagatctcgt ccgccgaggc
     1441 cggcctcgat aacatagaca ttgtgcgcga cttcttcggt ctgccacagg aagaggcgga
     1501 cgaaccaccc gccgccggtg aagagacgca ggtcatggag ggcgtggaag acgaggagga
     1561 agacgacacc caggttctgc gccgtccagg cccgctctcg gactccgaag actacttgga
     1621 accaggggat aaaaaggaac agtgagcatc ccgcgccgcc tggggcgcct cgcccagggt
     1681 ttcgtctcca gcctgcagga agacgagcgc ttccgcgaaa ggctccgtac agggcgcgag
     1741 cgtagcgagg ccctgagagg agccttcgaa gctgcctgga aaggcgcctc agaggagtgg
     1801 cgcaaggcgg aggagcgccg cgccgcacaa gaggcggccg cagaacagac ctggggatgg
     1861 cggtccgcga ccaccacatt ctccccaagg aaatacccgc ccaacgtctt ggccgcctac
     1921 gacaggctcg gcctcgtccc cagtgcttcc atggaagaag tagataggaa acgcagagag
     1981 ctggtcaagc gcttccaccc ggatcgcttc tccgatccgg agaagcgact acgtgccgag
     2041 cgcgtaacgg cagagatcaa cgccgcccac gacgccatag aacgccatgt agcgggttga
     2101 ggaaggttca ggccttcctc aacccgcgca ttttagcaag tcagcaaaga ctcccgaacg
     2161 caaatagctg aaatgctgac aagctaagaa accagttcgc cgcgcactat aaggcgattc
     2221 tcgaacgtcg gtattggggt gcaactttgg agcgagacta cggtcttgcc ctccacgggg
     2281 tagatcgccc agtagtcgtt tggctcgacg gtcatgaagt cgtagaccct gaacgtgtac
     2341 tttttccctt ccttgtcttc gatgaggatc tcatcacctg gtctcatctt cttgagctta
     2401 tagaagacgt agggcaccct ggtccgtggg aagccgaggg cgtgccccgt gataaacgtg
     2461 ttcgagcccg ctatccaggg caggccg
//
LOCUS       DASTKM010000075        24001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_544563, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000075 DASTKM010000000
VERSION     DASTKM010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 24001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24001
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544563"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VFQ10_02905"
     CDS             <1..853
                     /locus_tag="VFQ10_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9964320.1"
                     /translation="GGLSGSISGCVRRFDREKGWKIGPSPLNAGPRKGAPLYLQVERR
                     IEDLPLQGRYRAGDRIPPETELVDALGVSRITVRAGLARLVERGLLERRQGSGTFLVR
                     PPEGARLSAGLERLETYTVHAARLGLELGSSDLTIVAVGASPDEAAALEVARGTLLAR
                     VSRVLLIEGRPAAWMVDVVVENIIGVDDVRERFRPDAMLLDLLVSEGVPVGYSQMSIE
                     AVMISPGDHLGEKLGLKVHSAALSLIQTMYLIDGRPAQWSRDTFLPGKLNLHVVRELF
                     EVRNHPQ"
     gene            958..1296
                     /locus_tag="VFQ10_02910"
     CDS             958..1296
                     /locus_tag="VFQ10_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HET9964321.1"
                     /translation="MPYISWEDFEKVDVRVGTIVEAVPFPEARKPSIKLIVDFGPEVG
                     TRKTSAQLTAHYEPEGLVGRQVVAVLNFPPKRIAGFESEVLVLGVPDGNEDVVLLSPD
                     HRVPPGGRMF"
     gene            1366..2079
                     /locus_tag="VFQ10_02915"
     CDS             1366..2079
                     /locus_tag="VFQ10_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HET9964322.1"
                     /translation="MSETNLEQFREVTPEGRFTLQNSAMLKVRLENDQIQAKLGSMVA
                     YQGDVRFEHKSGGLGRLFKKAVTGEGVKLMAANGTGELFLAQDKRKIMVLDLQNERMI
                     VNGDNILAFEPGIDWDIRRVEGAGRLAGGLFNVVLEGTGKVALTSDGEPVLLDTSTPT
                     FADPESAIAWSGGVRTGVRSDVSFKTFTGRGSGESFQVAFEGAGWVLIQPSEGAAVPE
                     HSHAGEGGGGGGLGSLFED"
     gene            2115..2519
                     /locus_tag="VFQ10_02920"
     CDS             2115..2519
                     /locus_tag="VFQ10_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9964323.1"
                     /translation="MDQQRVPDLEELDEQQVRHFLAELFSDLSAEAEYEIRHEDYVME
                     MPQSGERIRGREKMRQFQEDYPAPPTIQLGRVVVREGLWVIEGVNDYGEGQVFTVVLI
                     IELKDGKMWRDTRYYAESFEAPEWRAQLAERF"
     gene            2687..3055
                     /locus_tag="VFQ10_02925"
     CDS             2687..3055
                     /locus_tag="VFQ10_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964324.1"
                     /translation="MESERVTREWLEGLDPAEFQEISAMISDLQRERATARRQKLEQR
                     RSAASKKAQNKRYLELERVKCGKKGCKKCASGELHGPYWYLYRPKENGPGRTSEYVGK
                     VLPESLAKEFDIPEDRRTAN"
     gene            complement(3146..4675)
                     /locus_tag="VFQ10_02930"
     CDS             complement(3146..4675)
                     /locus_tag="VFQ10_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HET9964325.1"
                     /translation="MQERERGADFNSKFTRRRFLTRRRFLQAAGGAAAWVALGGTLGC
                     DPAPRARATAASAPAGQAWNFRSRPDLHPPVIQVTKQARDTAPGYIFIAPKNGPEEAG
                     PGQDGCMILGNDGQPVWLRLLQNEDMDVMNFKAQTYKGETVLTWWEGLHTGYGQGEYV
                     ILDSSYRELARFGAGNGYQGDHHEFLITPEDTALITIYNRLPRDLVSEGDPLDGYVLD
                     GIAQELDIETGEVLFEWHSLEHVGLDESYTNHFDYFHINSIDVFDKDHLLISSRSTST
                     VYKVDRKTGQVVWRLGGKKSDFEMGPGTRTTFQHDARRQRDGTITIFDNGNVNRVDQS
                     RGIVVEVDEAKMSASLMREYTHPDRLLSDTQGNVQVLPKGNVLVGWGSAPFFSEFNHH
                     GELLFHAAFPTEGETYRAFRFPWSGQPTDDPAIAAELGTEDKVTIYASWNGATEVTTW
                     QVLAGSAPDQLEPLASAPRQGFETVITVRTTESYVGLNATNGSGKVLGTTNAIKLEDR
                     T"
     gene            complement(4796..7237)
                     /gene="leuS"
                     /locus_tag="VFQ10_02935"
     CDS             complement(4796..7237)
                     /gene="leuS"
                     /locus_tag="VFQ10_02935"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET9964326.1"
                     /translation="MSETKQRKTYDPTDLERRWQERWAESGLYKTEEDESKPKHYALT
                     MLPYPSGDLHVGHWYPMTPSDTRARFMRMNGYRVFFPIGFDAFGLPAENAAIQRGIQP
                     REWTYANIEQMRGQLKQMGAMFDFDSEVVTCDPEYYRWNQWFFVKFFEKGLAYRKEAP
                     VDWCPKDNTTLAREQVVGPDRRCERCGTPVIKKNLPQWLFKITDYAEELLDFSNIDWP
                     ERVETLQRNWIGRSEGTEIRFEIEGYGPVEVFTTRPDTLFGATFFVMSPEHPAVEKIT
                     TPEQAEEVRAYVERAARMSEIDRTDVTRDKTGVFTGAYAINPANREEIPVYIADYVLM
                     GYGTGAIMAVPGHDERDFEFARKYSIQIRTVITPPDWDGEALEVAYAGEGTMINSGKF
                     DGTPSTEGKERVTAWLEERGAGKATVTYRLHDWLISRQRYWGTPIPIVYCDECGIVPV
                     PEEDLPVLLPEESEFMPTGESPLKLDPDFYNTECPSCGGPATRETDTMDTFVDSSWYW
                     YRYLSPHYDEGPFDPERGEKWLPVDQYTGGIEHAILHLLYARFFTKVMRDLGLVDFDE
                     PMTSLRNQGNILGPDGQKMSKSRGNVVNPQEYVDRFGSDALRGFLMFIGPWNEGGPWD
                     GKGIEGVSRWLRRALSLVTGGDSSGGEADPTELERRTNRLIKKITEDLEAFRFNTSIA
                     ALMEHTNYLLAVRGRVGDDEWHEALRTFVTVLSPFAPHHAEETWAAMGEEYSVHEQAW
                     PGYEEDLIQAQEITLIVQVNGKLRDRIEAPADISEEAARELALSSARVRPHLEGKELR
                     KSIYVPGRLVNLVVG"
     gene            complement(7442..9283)
                     /locus_tag="VFQ10_02940"
     CDS             complement(7442..9283)
                     /locus_tag="VFQ10_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9964327.1"
                     /translation="MNTFRMLASPRVWGPILGVVVVLALIFYAYLAAVASPQENLKDL
                     PIALVNEDRGADLAGEDVNLGDRVVENVTNPDSPAAGTVDWVRPDTRSEALKAIGNGD
                     YYGAIVIPEDYTQRISGLAGPPRLPIAVVVEDQGAEINGRPVELGGEVANTITSPDSP
                     APDFVQWERLEGRGEAKKSLESGEYYAAIVIPKNYSQHLVSLSGPPVGVPPLAQPPVL
                     EPAEIELLTSPAVRPSTTAQIENAFTGIVGGVSGATSEQIFEGLSEQGVPVPQGAGAV
                     ISDPVKGNVSEADVSGEAGPLPKAPEPARIEVFTNPSAGQAVATPVRTISTGIGDAVS
                     MATSERLSSAAAEQGAQLTPEVAAVIGDPVRADVTEAQPVGPDSGNGQSPFFLAFLVN
                     LSGLIGGAVIFFLAGGAAERMEGLGLRPSRTGLWTVRLGLVFGLLIAGAELLVAFGLL
                     GVEHEASTAQVCLFLTLATTAAVSVTMLFAVAFGPAGIAISAVLNVILGLVSSGGLAP
                     LEALPPFYQAYADWLPLRYVIDGLRSLLFYDGSLDAARLESGWRDSLWFFGGGGRSEA
                     AGLEDAVWMIGAYLAVAAVLGYLISLFRDVFARRGRKEAGRVAETAG"
     gene            complement(9280..10548)
                     /locus_tag="VFQ10_02945"
     CDS             complement(9280..10548)
                     /locus_tag="VFQ10_02945"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9964328.1"
                     /translation="MTLEVRHATCCVIGCGPAGAMLGLMLAREGVDVLVLEKHGDFLR
                     DFRGDTLHPSTMEIMDELGLAEGLLDLEHTKAPRIEARLPGRTVTVADLSHLKTRYPF
                     ITFMPQWDFLDYVTGEAKRYPNFRLEMNAEAKDLILEDGIVRGVRYETPQGPCEARAL
                     LTVAADGRSSPFREQTGLKMVRTSPPIDVLWFRLSRRDGDPEDTFGYAGGGRFMVVIN
                     RGEYWQIAYVIRKGDYQKVREDGLEDFRRSVGETIPDLADRTWELQDWEAVKLLSVQA
                     DRLRRWHRPGLLCIGDAVHAMSPVGGVGINLAIADAVAAANALAGPLGEGRVHSRDLI
                     AVQLRRELPTRAIQAFQSFAQRRVVSSSLGPDGAPSIPVPARMLLSLRPVRDLAARII
                     AFGIWPEHVKRQDASERIGRTQVARKESVE"
     gene            complement(10569..11210)
                     /locus_tag="VFQ10_02950"
     CDS             complement(10569..11210)
                     /locus_tag="VFQ10_02950"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9964329.1"
                     /translation="MVATSEELGPRARAKRDQILAGARRVFLRDGFAASSTDAIAAEA
                     KVSKRTLYVYYPSKEDLFVDVLRKLTIENPQTRALESMEGMSPENREELRRDLLELAQ
                     KIVTTMMQPDYLALLRTTIADIHRFPQLAGLFRATVPERAMSSFAVFIERTRDRRVIR
                     EDVNGEAAARMFVGPLLTYAVLDGLFVAGGPPRKPTSGRIEEIVDMYVEAISP"
     gene            complement(11304..11996)
                     /locus_tag="VFQ10_02955"
     CDS             complement(11304..11996)
                     /locus_tag="VFQ10_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HET9964330.1"
                     /translation="MASQVLTALLVTGAFAALAYALGMISPSGALGGLLVGATIYASL
                     GPRGFAVLALFVIGGSLLTRFGYGSKQRTGTAQEHGGRRSARNALANCAVATLCAILA
                     ATGSEPFAAAFVASLGAAFADTAESEIGQLFSRTPRLISTLQKVPPGTDGAVSFPGTL
                     AGAGAAVLTALLGLSLGMLETPASALLVAIAAFLGTLADSLIGALSPRAGNELTNLLC
                     TLVAALLALILT"
     gene            complement(12004..12423)
                     /locus_tag="VFQ10_02960"
     CDS             complement(12004..12423)
                     /locus_tag="VFQ10_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964331.1"
                     /translation="MDYESPLASRVAGDVWSGLSRAALEAGFTKTTAHWESLREPRIP
                     REDPPVHVFAIGQDRPDAIDAVGAVGDPGAPHLYGVIVAVPSKPSPLAGSLLYQGVER
                     LTRTGVKAGMVEPALLHAIPAECERYARRLLERLGSP"
     gene            complement(12477..12812)
                     /locus_tag="VFQ10_02965"
     CDS             complement(12477..12812)
                     /locus_tag="VFQ10_02965"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9964332.1"
                     /translation="MNASRSDMFRKLLQRDPRNPMVLYSLGNELFKEGRYREARDHLQ
                     RAVANKPDYSVAYRMLGRAHYELHENAEARDIFLKGREVAQGNGDLQTVKEIDVFLRR
                     LQKREVGEG"
     gene            12889..13212
                     /locus_tag="VFQ10_02970"
     CDS             12889..13212
                     /locus_tag="VFQ10_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9964333.1"
                     /translation="MDIGPLLRELAEGTALEPFRALIEPLQEHDKTRRSDLVLTLRVY
                     FATGGNASEAADRLFLHRNSMLYRLERIQKLTGLDLRDHRVALALQLGLLAKERGERI
                     ATEYP"
     gene            13193..13414
                     /locus_tag="VFQ10_02975"
     CDS             13193..13414
                     /locus_tag="VFQ10_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET9964334.1"
                     /translation="MQLSTRNRLPGTITEVVKGEAAAKVTLQVGDNHVVALITRESAD
                     ELGLEPGKQATALVKATDVMILTEGGDVA"
     gene            complement(13446..14285)
                     /locus_tag="VFQ10_02980"
     CDS             complement(13446..14285)
                     /locus_tag="VFQ10_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9964335.1"
                     /translation="MKGRDKVGSSTRLPRGSDTLASGLALASLALLVGFVSLPLLSLV
                     VWTVDKGTWRAMASPVAVDALLLSLRTTAITMAILLLIGTPAAYVLARAEFRGSRVIN
                     TLIDIPVVLPPSAAGIALLLAFGRLGLVGQYLNVFGITLSFTTAAVVMAEVFVAAPFY
                     VRQAATGFGAVDRAVEEAAMVDGAGHWRRFFRVTIPLALPALVAGALTAWARALGEFG
                     ATIIFAGSFRGVTQTIPLAIFAEFQRDLDAAVALSVLVLGFAFAVILAVRCLTARSTK
                     DRA"
     gene            complement(14282..15079)
                     /gene="modA"
                     /locus_tag="VFQ10_02985"
     CDS             complement(14282..15079)
                     /gene="modA"
                     /locus_tag="VFQ10_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9964336.1"
                     /translation="MVSGVALAGIAGGCGGVSGGESGGGGKQQGGTLTVLAASSLIDA
                     FGVLANRFEEQNPGVKVKQSFESSSTLLAQIQQGAPADVFASAAKEEMNTAVKEGLVK
                     GEPEVFVKNREIVMVPKDNPANIRRFRDVAKPDVKLVLAGKDVPAADYALEILGKAKG
                     EYGADFEKDVLSNVVSRESDVRASVNRVVVGDADATFGYASDYTPDIRDRVEIVRIPP
                     DLNIVAPYPIAALEDAKSPELAKKWVELVRSEEGQGVLQKWNFEPAA"
     gene            complement(15260..16441)
                     /locus_tag="VFQ10_02990"
     CDS             complement(15260..16441)
                     /locus_tag="VFQ10_02990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HET9964337.1"
                     /translation="MGVLSLALVLAGAEGPARAQSADEDMSVARSTRVPLLEAKISDQ
                     DAALRGMIEEISTVGAELDRAQSRVDDAGARVDDLRRQTRQLKRQIAEQDAAFREAQA
                     RYRAQAQAAYKGGSLEGLAALLDGWFFDSWEGVAGTGDPVVARILLDSRQNLAAYRES
                     RQTLRNTQRQIAQKRRDYNAAIEEQQATTADLRVREQALDQSIDRLGTNNSRTEARLQ
                     KLKAAERARILKTTAATGGGEVGSEHELEVAREDIVATSVEPTSENAYMELYRASATK
                     YGFGPDWYILAAVGKVESNHGQAMGPSSAGAMGPMQFMPTTWETSGVDGNGDGVANVM
                     DPEDAIPAAASYLEDCGAPRDWYRALYSYNHADWYVKKVLAVAEAYRRLAEDESVGPY
                     I"
     gene            complement(16623..17489)
                     /locus_tag="VFQ10_02995"
     CDS             complement(16623..17489)
                     /locus_tag="VFQ10_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET9964338.1"
                     /translation="MGKRGLGRGLSALIATGESVGGLKFEELPVSAIRPNSRQPRTSF
                     PEAGIRELAASIQEVGILQPLVVRPTPGGFELIAGERRLRAAKEAGMERVPVLIRQAG
                     EDESIELALVENLQRENLGPLETAAAYQALMDGFGLSRERLARRLGKSRAAVTNTLRL
                     VHLPEKIQALLGDGKLSEGHARALLGLETEEQMTRLAQRVVDEKLSVRKTEELVREML
                     GNPRESSSAMPQRSNATPDEFHQASRLMREALELPVRVKGLKRGGKVEIRFRQTEELQ
                     ALVSLLTSRNDT"
     gene            complement(17482..18246)
                     /locus_tag="VFQ10_03000"
     CDS             complement(17482..18246)
                     /locus_tag="VFQ10_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET9964339.1"
                     /translation="MPAVRTIVAIVNQKGGVGKSTTAINLAAYLAGKGERILVVDMDP
                     QGNATSGLGVSPGPGGCMYDVLLEGKPLRDVAVKTEIEGLDVAPATINLVGAEVELVS
                     SLAREFKLKRALEKQSSYDRVLLDCPPALDLLTLNALTAADEALIPIQCEYYALEGLT
                     QLMQSIRMVREELNPGLEVGGVLLTMFDPRTNLAHQVAEEVRSFFGERVFHTIVPRNV
                     RLSEAPSFGMPIALYAPKSTGAEAYSAVADEVVNRG"
     gene            complement(18273..19013)
                     /gene="rsmG"
                     /locus_tag="VFQ10_03005"
     CDS             complement(18273..19013)
                     /gene="rsmG"
                     /locus_tag="VFQ10_03005"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET9964340.1"
                     /translation="MKRPMAALGLMEAQAAAWGLRLCHDRRGHLQEYARLLASYDLAN
                     VIGTRDVDRILMDHVLDSLSCFLHEPLFRAWRLADVGSGGGLPGIPIKILRPDLAMTL
                     VESTGKKASFLRHAVGGLSLRGVEVANTRVEDLGRARGHRGAYDVVTCRAVARLSVVA
                     EYCVPLLETGGCAIAMKGRLEPEELAEGNRAVDALGAKVAETTRVPMLPEVGEKDRNL
                     VILEKIRETPEPYPRLSGIVAKRPLGVR"
     gene            complement(19117..19902)
                     /gene="jag"
                     /locus_tag="VFQ10_03010"
     CDS             complement(19117..19902)
                     /gene="jag"
                     /locus_tag="VFQ10_03010"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HET9964341.1"
                     /translation="MTDSMEFYASTIEEAVEKAALELGLSSEQVSYRVLDEGNSGFLG
                     IGARDARIIVQASAPHASGEDEHPETTPAENVVVDEPEPEPSENAGSSTLSEVYGEQS
                     ESEQVEAPQELLDEIRALVSSVLDAMGFEARVEVYDAGGFIAADVAPDNTALFIGQKG
                     ETIDALQYLVNVSAFRDRPFFKRIVLDAEGYRQRRVEAIQGMAHRTARRAVRERRTVE
                     MPPMNSSERRVVHLFLSENPGVTTESEGSGDNRRVKVSPITQA"
     gene            complement(19899..20756)
                     /locus_tag="VFQ10_03015"
     CDS             complement(19899..20756)
                     /locus_tag="VFQ10_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET9964342.1"
                     /translation="MLNAIADFFQNLFSPVVSVLGAVLLYFHQTLGAPWWLAIVLLTV
                     IVRSLLFPLTVKQVRSMRAMQDLKPHMDRVRAQFKENPQRQREEMAKVYQEQGVNPLG
                     GCLPILVQMPIFIGIFYVIRQFGGYSFGDRTVPAQYPSFHEGGILWFQNLSVADSTYL
                     LPIISAVTMLAATEITAKNIDPQQRWLMRLLPIGFTIFLLNFPAGLFVYWITSNLVTL
                     GQNFVIYNYGPGKKPTDGAKGIDGGKLKATDGQARNSGGEQSSERSQEEIAKQAAKVA
                     KRKRRKKKK"
     gene            complement(20767..20982)
                     /gene="yidD"
                     /locus_tag="VFQ10_03020"
     CDS             complement(20767..20982)
                     /gene="yidD"
                     /locus_tag="VFQ10_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET9964343.1"
                     /translation="MSPKKLSTGLIRLYRRYLSPMMTPSCRYTPSCSLYTLQAIEKYG
                     VPRGILMGVLRVLRCHPFSKGGFDPVK"
     gene            complement(20995..21333)
                     /gene="rnpA"
                     /locus_tag="VFQ10_03025"
     CDS             complement(20995..21333)
                     /gene="rnpA"
                     /locus_tag="VFQ10_03025"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET9964344.1"
                     /translation="MRLTDSPEFERVYRQGTAYRGRLFSVHAFPNEHGTPRLGLSISR
                     KVGNAVTRNAVRRRLREVFYSCISELSGNLDLVVSARPAAAEATFEELQEEFSKSLGR
                     VGGPVGRRPV"
     gene            complement(21355..21489)
                     /gene="rpmH"
                     /locus_tag="VFQ10_03030"
     CDS             complement(21355..21489)
                     /gene="rpmH"
                     /locus_tag="VFQ10_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET9964345.1"
                     /translation="MKRTYQPNRRKRAKTHGFRKRMSSVGGRRVVADRRRRGRKRLAV
                     "
     gene            21806..22654
                     /locus_tag="VFQ10_03035"
     CDS             21806..22654
                     /locus_tag="VFQ10_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9964346.1"
                     /translation="MFLGYSATMRGALNSLLGMMHAQRTPGETDQAGVGVRIAVYPRS
                     GTPEITEVSASDPGSATRKLTKIVSEKVREAGGRVPEESIREVVENLIHAGYQGVVIS
                     VLEDGNVVRVSDKGPGVENKSRAMEFGFSGAAPGALGEIRGIGAGLGIARAAAEKVGG
                     TVTIEDNIGGGTVATVSAEATETVTGEGEAASVPPPQRRYPDGVPRMNISERQQKALI
                     TVLECGEVGPSTVADRLEISVSTAYRDLSVLEEHGLVLADESGKRLISPLGRDLVEAI
                     INAWVR"
     gene            22657..24000
                     /gene="dnaA"
                     /locus_tag="VFQ10_03040"
     CDS             22657..24000
                     /gene="dnaA"
                     /locus_tag="VFQ10_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET9964347.1"
                     /translation="MAGKAEQVWTEVLDRASEHIDVSSLRVWFEGIRPVALYEDQLEI
                     SVPNTFAKEYIESRFRPLLEEVLDDVVGQETSLVVSVGGPGVHTGNGTAAEGAESVLN
                     ARAPRSFRAKYTFDTFVIGAGNRFAHAATLAVAETPGLVYNPLFIYGGVGLGKTHLLR
                     AVGHYVEDQDPSMRIRYVTCEQFTNDFINSMRDNAPLEFQKRYRENDVLLVDDIQFLE
                     NKVETQEAFFHTFNALYEENKQIVIASDRHPRYIQTLENRLVSRFEWGLVTDIQPPDL
                     ETRIAILRKKALMDHLEVDDDVLTFIASKVSTNIRELEGALVRILAYASLYGRQVSAD
                     LAEEVLRDILPDQAYREIPIELIQHEVCRYFGIAKADLVGQSRSKGFAYPRQVAMYLS
                     RELTDESLPKIGKAFGGRDHSTVMHATAKIANLINSDRDVFSQIHEITQQVKSKR"
BASE COUNT         4510 a         7461 c         7331 g         4699 t
ORIGIN      
        1 cggcggtctg tcgggtagca tttcggggtg tgtcagacgt ttcgatcgag agaaagggtg
       61 gaagatcggg cctagtccgc taaacgcggg accgaggaaa ggggctccac tctacctcca
      121 ggtcgagcgg cgcatcgagg atcttccgtt gcagggtcgc tacagagccg gggaccgcat
      181 accgcccgaa accgagttgg tcgacgctct gggcgtctcg cggataacgg tacgggccgg
      241 gctggcgcga ctcgtagaac gggggttgct ggagcggcgc cagggtagcg ggacgttcct
      301 agtgcggcca ccggaaggag cgcggctctc ggcaggcctg gagcggctgg agacttacac
      361 ggtccacgct gcaaggctgg ggcttgagct cggtagctcg gacctgacca tagtggccgt
      421 cggtgcctcg ccggatgagg ctgcggcatt ggaggtcgcc agaggtacgc tgctcgccag
      481 ggtctcgcgc gtcctgctca tcgaaggtag gccggccgcg tggatggttg acgttgtggt
      541 cgagaacatt atcggggttg acgacgtcag ggaacggttc cgccctgacg ctatgctgtt
      601 ggatctcctg gtctccgaag gtgtgcccgt gggctacagt cagatgtcca tcgaggccgt
      661 catgatcagt ccgggagacc atctcggtga aaaactcggc ctgaaagtcc attccgcagc
      721 tctctcgctg atccagacca tgtacctgat agacgggcgt cccgcgcagt ggtcacgcga
      781 taccttcctg ccggggaaac tcaacctcca cgtcgtgaga gagctcttcg aggtcaggaa
      841 tcatccgcaa tagttatcag gtgtcaggct acaggtatat acctggatcc gggactgcgc
      901 cggttctcga aacagcttgc tgatacctgt agcctctaga cataggagga gccccgattg
      961 ccttacataa gctgggaaga cttcgagaag gtggatgtac gggtcggcac gatcgtcgag
     1021 gccgtacctt tccccgaagc caggaagcct tcgataaaac tcatcgtgga cttcggaccg
     1081 gaggtcggga ccaggaagac cagcgcccag ctcacggcgc actatgagcc tgagggactc
     1141 gttggtcggc aggtcgtcgc tgtgctcaac tttccgccta agaggatcgc aggcttcgag
     1201 agcgaggtgc tcgtcctcgg cgtacctgac gggaatgagg acgtggtgct gctctcccca
     1261 gatcaccggg tcccgccggg tggcaggatg ttctaggaaa cttctggacg ggcgcttcgt
     1321 acccaggtag tatcgtttac ttcgatcgga aagggaggta gcgcggtgag tgagacgaac
     1381 ctggagcagt ttcgggaggt tacgccggag gggcggttca cgctgcagaa ttcggcgatg
     1441 ctcaaggtgc ggttggagaa cgaccagatc caggccaagc tcggatcgat ggtcgcctac
     1501 cagggcgacg tccgcttcga gcacaagagc ggcggcctgg gacgtctctt caagaaggcc
     1561 gtcacgggcg agggcgtcaa gctaatggcg gccaacggga cgggcgagct gttcctcgcg
     1621 caggacaaac gcaagatcat ggtcctggac ctccagaacg agcgcatgat cgtcaacggt
     1681 gacaacatcc tcgccttcga accaggcatc gactgggaca taaggcgggt cgaaggcgcc
     1741 ggccgtctcg caggcggcct cttcaacgtc gtcctggaag gcacaggtaa ggtcgccctc
     1801 acctcggacg gggagcccgt actcctcgac acctcgaccc caaccttcgc cgaccccgaa
     1861 tcagccatcg cctggagcgg cggtgtgagg acgggcgtaa ggagcgacgt ctccttcaag
     1921 accttcaccg gacgcggcag cggagagtcg ttccaggtag ctttcgaggg ggcaggctgg
     1981 gtcctcatac agccctcgga aggcgctgcc gtccccgagc attcccacgc cggcgaaggc
     2041 ggtggaggcg gcggtctcgg gtccctcttc gaggactagc cggcctcacc gcaactgccg
     2101 caggagaagg acagatggat cagcaacggg tgcccgatct cgaagagttg gacgagcaac
     2161 aggtgcgcca cttcctcgcc gaactttttt ccgatctaag cgccgaagcg gaatacgaga
     2221 tacgccacga ggactacgta atggagatgc cccagtccgg agagcgcatc aggggccggg
     2281 agaagatgag gcagtttcag gaggattacc ccgctccccc aaccattcag ctaggaaggg
     2341 tggtcgtcag agagggcctg tgggtcatcg agggcgtcaa cgactacggc gagggacagg
     2401 tgtttaccgt cgtgctgatc atcgagctca aagacgggaa gatgtggcgt gatacccgct
     2461 actacgcaga gtcgttcgag gcgccggagt ggcgggccca gttggccgaa cggttttagg
     2521 aggtttcatg atgttctctg ggaacagaac tggactaaca ggcgattctc taccttgttg
     2581 gttcgaccaa tcagaagcgc gctatactgg ctggagtagg tcttatgtac agtacggaga
     2641 actcctgtga taatgtgtac ataagttagc tggtgtggaa ggtgtgatgg aatcggagcg
     2701 ggtaaccagg gagtggttgg aggggctgga tccggcggag ttccaggaga tctcggccat
     2761 gatctccgac ttacaacgcg agcgcgcaac ggcgcggcga cagaaactcg aacagcgtcg
     2821 atccgcagcc agcaagaagg cccaaaacaa gcgctacctt gaactggaac gcgtcaaatg
     2881 cggcaagaaa ggctgcaaga agtgcgccag cggagagctt cacggccctt actggtacct
     2941 gtaccggccc aaggagaacg gaccaggacg taccagcgag tatgtcggca aggtgctgcc
     3001 cgagtctctc gccaaagagt tcgacatccc tgaagaccgg cggacggcga actagccgac
     3061 ctgtttctta tgtatggcaa actgcccact ttttgcctag cttacataag atgagcagat
     3121 gagatttgcc gtcgatactt gaaggctagg tacggtcctc cagcttgatt gcgttggtgg
     3181 taccgagcac cttacccgag ccgttcgtgg cgttcagacc gacataggac tcggtggtgc
     3241 gtactgtgat cacggtctcg aagccctgac ggggagctga tgcgaggggt tctagttgat
     3301 ctggggcgct cccggccaac acctgccagg tggttacctc tgtagcgccg ttccagctgg
     3361 cgtagatggt taccttatcc tcggttccga gctcggcggc gatggcaggg tcgtcggtag
     3421 gttgcccact ccacgggaaa cggaaggccc tgtaagtctc gccctcggtt gggaaggcgg
     3481 cgtggaaaag cagctcgcca tgatgattga actcagagaa gaacggcgcg cttccccagc
     3541 ctacaaggac gttgcctttc ggcaatacct gtacgttgcc ctgggtatca gagagcaacc
     3601 tgtcggggtg ggtgtactca cgcatcaggc tggcactcat tttagcttcg tcgacctcga
     3661 ccactatgcc gcgggactga tctactcggt ttacgttgcc gttatcgaag atggtgatag
     3721 taccgtctct ctggcgacgg gcgtcgtgct ggaaggtggt tctggtacca ggacccatct
     3781 cgaagtcgct tttcttcccg ccgagccgcc acacgacctg gcccgtcttt cggtcgacct
     3841 tgtacacggt agaggtcgag cgggaggaga tgagcaggtg atccttatcg aatacgtcta
     3901 tggagttgat gtggaagtag tcgaagtggt tggtataaga ctcatctagg ccgacgtgct
     3961 ccaggctgtg ccactcgaag agcacctcgc cggtctcgat gtccagctcc tgggctatcc
     4021 cgtcgagcac ataaccgtct aagggatcgc cctccgaaac gaggtctctg ggcaacctgt
     4081 tgtagatcgt gatcagggcc gtatcctcgg gcgtgatgag gaactcgtgg tggtccccct
     4141 ggtagccgtt gccggcgccg aaccgggcta gttcgcggta ggagctatcg aggatcacgt
     4201 actcgccctg gccgtatcct gtatggagtc cctcccacca ggtgagcacc gtctctccct
     4261 tgtatgtctg cgctttgaag ttcatcacat ccatatcctc gttctgcaag aggcgcagcc
     4321 aaacgggctg tccgtcgttg cccaggatca tgcatccgtc ctgccccgga ccggcctcct
     4381 cgggcccgtt cttcggagcg atgaagatat agccgggagc agtatcgcgc gcctgcttgg
     4441 tcacctggat gaccggcggg tggagatccg gacgcgagcg gaagttccat gcctgtcccg
     4501 ccggtgcgga tgcggcggtc gcccgggcgc gtggggccgg atcgcagccc agggtgccgc
     4561 ccagcgcaac ccacgcggcg gcgccaccgg cggcctgcag gaacctcctt cgcgtgagga
     4621 acctccttcg cgtgaacttg gagttaaagt cggcacctct ttctcgctcc tgcacaggct
     4681 acttcttcac ccatcttcct gaagttacgc aaggtacact gcttcaccct acgcctacaa
     4741 cagcatcgca tagtcttccg agtctctctc gcagcggcgc ggggttcctg gaccgctatc
     4801 ctacgacgag gttgacgagc ctgcctggca cgtagatgct cttgcggagc tctttgcctt
     4861 cgaggtgagg acggaccctg gcgctcgaca gtgccagctc tctggccgcc tcctcggaga
     4921 tatctgctgg ggcttctatg cggtcgcgca gtttgccgtt gacctggact atgagcgtta
     4981 tctcctgagc ctggatcagg tcttcctcat agcctggcca cgcctgctcg tgcacagagt
     5041 actcctcccc catcgcggcc cacgtctcct cggcgtggtg cggcgcgaac gggctgagga
     5101 ccgtgacgaa ggtccgcaag gcttcgtgcc actcgtcgtc cccgacgcgg cctcttaccg
     5161 cgagaaggta gttagtgtgc tccatgagcg cggcgatgga cgtgttgaag cggaaggctt
     5221 cgaggtcctc tgtgatcttc ttgatcaggc ggttcgtccg gcgctccagc tccgtcggat
     5281 ctgcctcgcc gccagaggag tcccctcctg tcacgagcga gagggcgcgc ctgagccagc
     5341 gcgagacacc ctctatgccc ttgccgtccc agggaccgcc ctcgttccac ggaccgatga
     5401 acatcaggaa accccggaga gcatcggagc cgaagcggtc tacgtactcc tgcgggttga
     5461 ccacgttgcc tcggctcttg ctcatcttct gcccgtcggg tccgagtatg ttcccctggt
     5521 tgcgaaggct ggtcataggc tcgtcgaagt caaccagacc aagatccctc atgaccttgg
     5581 tgaaaaagcg ggcgtagagc aggtgcaaaa tggcgtgctc gatgccgccg gtgtactggt
     5641 cgacggggag ccacttctct ccgcgctcgg gatcgaaagg cccctcatcg tagtgcggcg
     5701 agaggtagcg gtaccagtac cacgatgagt ccacgaaggt atccatagtg tccgtctcgc
     5761 gcgttgccgg gccgccgcaa ctgggacact cggtgttgta gaagtcaggg tcgagcttga
     5821 ggggcgattc tcccgtcggc ataaactcgg attcctctgg gagcaggacc ggcaggtcct
     5881 cttcggggac tggtacgatg ccgcactcgt cgcagtagac tatggggatc ggggtccccc
     5941 agtagcgctg gcggctgatc aaccagtcgt gaagcctgta ggtgaccgta gccttcccgg
     6001 caccgcgctc ctcgagccag gccgtcaccc tctctttgcc ctcggtgctc ggggtgccgt
     6061 cgaacttgcc tgagttgatc atcgtgccct cgccggcgta agccacttcc agcgcttcgc
     6121 cgtcccagtc cggcggcgtt atcacggtcc ttatctggat gctgtacttc cttgcgaact
     6181 cgaagtctcg ctcgtcgtgg cctgggacgg ccatgatcgc gccagtaccg tagcccatca
     6241 ggacgtagtc ggcgatgtag acggggatct cttctctatt ggccgggttg atcgcgtaag
     6301 cccccgtaaa cacgccggtc ttatccctcg tcacgtcggt gcggtcgatc tcgctcatcc
     6361 tggcggccct ctcgacgtag gcgcgcacct cttcggcctg ctccggggtc gttatcttct
     6421 cgaccgccgg gtgctccggg ctcataacga agaacgtggc gccgaagagc gtatctggtc
     6481 gggtagtgaa gacctcgacg gggccgtagc cttcgatctc gaacctgatc tcggtcccct
     6541 cagagcgccc gatccagttt cgctgcagcg tctcgacccg ctcgggccag tctatgttcg
     6601 agaagtcgag gagctcctcg gcgtagtccg tgatcttgaa gagccactgc ggcaggttct
     6661 tcttgatgac cggcgtcccg caccgctcgc agcgcctgtc tgggcccacc acctgctcgc
     6721 gggccagcgt cgtgttgtcc ttcgggcacc agtcgacagg ggcttcctta cggtacgcca
     6781 gacccttctc gaagaacttc acgaagaacc actggttcca gcggtagtac tcggggtcgc
     6841 aggtcacgac ctcggagtcg aagtcgaaca tcgcgcccat ctgcttgagc tggccgcgca
     6901 tctgctcgat gttcgcgtag gtccattcgc ggggttgtat gcccctctgg atcgctgcgt
     6961 tctcggcggg cagcccgaag gcgtcgaagc ctatcgggaa gaacaccctg tagccgttca
     7021 tccgcataaa gcgcgctctg gtgtccgagg gcgtcattgg gtaccagtgc ccgacgtgca
     7081 gatcaccact cgggtaaggg agcatcgtga gcgcgtagtg cttgggcttg ctctcgtcct
     7141 cctcggtctt gtacaggccg ctctcggccc accgctcctg ccagcggcgt tcgaggtccg
     7201 tgggatcgta tgtctttctc tgtttcgtct cgctcacgag agactccttc tgttaagtgc
     7261 ttggggtgat tctagatcag gggaaatccg cgcagggctt tatagtcggg agtccgactt
     7321 ctgaatcttg tgatccctgg cgcacataca ctaaatatag catcaggtat ggcctttaca
     7381 aaaggagggt gccgcccgga agccactccg ggcggcaagt aagtacctct cggcgagagt
     7441 tctaccctgc cgtctcagca actctcccgg cctctttacg tccgcgccgg gcgaacacgt
     7501 cacggaacaa cgagatcagg tacccgagca cggcggcaac cgccaggtac gcgccgatca
     7561 tccacacggc atcctcgagg cctgcggctt cggatctccc gcccccaccg aagaaccaca
     7621 gggaatctct ccaaccgctt tccaggcgag ctgcgtccag ggagccgtcg tagaagagca
     7681 gggagcggag cccgtcgata acgtagcgca ggggcagcca gtcggcgtac gcctggtaga
     7741 agggtgggag agcctcaagg ggcgcgaggc ctccagagga gacgaggcca aggatcacgt
     7801 tcaaaaccgc gctgatggct atgcctgcgg gcccgaacgc caccgcgaag agcatggtca
     7861 cggacacggc agccgtcgtg gcgagcgtga ggaacaggca cacctgcgcc gtgctcgcct
     7921 cgtgctcgac gccaagaagg ccgaacgcga ccaacagctc ggccccggct atgagcaaac
     7981 cgaatactag cccgagccgt accgtccaca atcccgtgcg ggaagggcgt aggcccagcc
     8041 cctccatccg ctcggcggcg ccgccggcga ggaagaagat gaccgcgccg ccgatcaggc
     8101 ccgagaggtt gacaagaaac gcgaggaaga aaggcgattg gccgttacca gaatcgggtc
     8161 cgaccggctg cgcctcagtg acgtcggccc ttacagggtc gccgatcacg gctgcgacct
     8221 cgggcgtaag ttgcgccccc tgctccgctg cggccgagga gagccgctcg ctcgtcgcca
     8281 tcgagaccgc atccccgatc cctgtggaga tggtccgtac gggtgtcgcg acagcctgcc
     8341 cggccgaagg attcgtgaac acctcgatcc gggccggctc aggggccttg ggaagcggcc
     8401 cggcttcacc ggatacgtcg gcctcggaga cgttcccttt gacggggtcg gagatcaccg
     8461 ccccggcccc ctgtggcacg ggcacaccct gctccgagag cccttcaaat atttgctcgc
     8521 tcgtcgcgcc agagaccccg ccgacgatgc cggtgaaggc gttctcgatc tgggccgtag
     8581 tagagggcct taccgcaggg ctcgtcaaca actcgatctc agcaggctcg agtacaggtg
     8641 gctgcgccaa aggaggcaca cccaccggcg gaccggacag gctcaccaga tgctgcgagt
     8701 agttcttcgg gatcacgatc gcagcgtagt actcgccgct ttcgagactc tttttggcct
     8761 cgccgcggcc ctcgagccgc tcccattgca cgaaatccgg ggccggggag tcgggagaag
     8821 tgatcgtgtt cgccacctcc ccaccgagct ctacaggccg gccgttgatc tccgccccct
     8881 ggtcctcgac tactacggct atgggtaacc tcggcgggcc ggccaggccg gagatccgct
     8941 gcgtatagtc ctcggggatc acgattgcgc cgtagtagtc gccgttgccg atggccttca
     9001 gcgcctcgct gcgggtgtca ggacgcaccc aatcgactgt gcctgctgcc ggcgagtccg
     9061 gattcgtgac attctcgaca acgcggtcgc ccaggttgac atcctctcct gcgaggtctg
     9121 cacctctgtc ctcgtttaca agggcgattg gcaggtcctt gaggttctcc tgagggcttg
     9181 cgacggcggc caggtaagcg tagaagatca gggcaagaac cacgaccaca ccgagaatgg
     9241 gaccccaaac cctcgggctc gcgagcattc taaacgtgtt cattccaccg actcctttct
     9301 tgctacctgc gtccggccga tcctctctga ggcgtcctgg cgctttacgt gctcgggcca
     9361 gatgccgaaa gcgatgatgc gggccgcaag atctcgcacc gggcgcaaac tcaagagcat
     9421 cctcgctggt acgggtatcg aaggcgcccc atccgggccg agggacgagg agacaacacg
     9481 ccgctgggcg aaggactgga aggcctgtat cgctcgggtc ggtagctcgc gcctgagctg
     9541 caccgctatc aggtcgcgcg aatgtacgcg cccttcccca agcggtcccg ctagggcatt
     9601 cgccgcggcg accgcatctg ctatggcgag gttgatgccg acgccgccga cgggagacat
     9661 ggcgtgcacg gcgtccccga tgcacagtag ccctggcctg tgccagcgcc gcagccggtc
     9721 agcctgcaca gagagaagct ttacggcctc ccaatcttgc aactcccatg tacgatcggc
     9781 gagatctggg atcgtctcgc cgacggatct tcggaagtct tccagaccgt cctcgcgcac
     9841 tttctggtag tcgcctttcc tgattacgta ggcgatctgc cagtattcgc cgcggtttat
     9901 aacgaccatg aaacgcccgc ctccagcata cccgaaggta tcctcggggt ctccatcccg
     9961 acgcgagagc ctgaaccaga gcacgtcgat gggcggagag gtcctgacca tcttcaggcc
    10021 ggtctgctct ctgaagggag aggaccgtcc gtcggcggcg accgtgagga gtgcccgcgc
    10081 ctcacaggga ccttggggtg tctcgtagcg cacgccgcgt acgatcccgt cttccaggat
    10141 caagtctttc gcctcggcgt tcatctccag gcggaagttc gggtagcgct tcgcttctcc
    10201 tgtcacgtaa tccaggaagt cccactgggg catgaaggtg atgaacgggt agcgcgtctt
    10261 caggtggctg aggtcagcga ccgtaacggt ccttcccggg aggcgggctt cgatcctggg
    10321 ggccttggtg tgctccaggt cgagcaagcc ctccgccagg ccgagctcgt ccattatctc
    10381 catggtcgag gggtgtagtg tgtccccgcg gaagtcccgc aggaagtccc cgtgcttctc
    10441 gagcaccagc acgtcgaccc cctcgcgcgc cagcatgagc ccgagcatcg ccccggccgg
    10501 gccgcagccg atcacacagc atgtggcgtg ccggacttcg agcgtcatct gcgtctcctt
    10561 tctcaccctc aggggctgat cgcctccacg tacatatcca ctatctcttc gatgcgcccg
    10621 gacgtgggct ttcgcggcgg tcctccggcg acaaagaggc cgtcgaggac cgcgtatgtg
    10681 agcaaaggac caacgaacat gcgcgctgcc gcctctccgt tcacgtcttc cctgatcacc
    10741 cttcgatccc tggtccgctc tatgaagacg gcgaagctgc tcatggctcg ctcgggtacc
    10801 gtggcgcgga agagtccggc caactgcgga aacctgtgga tgtccgcgat ggtcgtcctg
    10861 agcagcgcga gatagtccgg ctgcatcatg gtcgtgacaa tcttttgcgc cagctcaagc
    10921 agatcccggc gtagctcttc tcggttctcc ggtgacatcc cctccatgga ctccagtgcc
    10981 cgcgtctggg ggttctcgat ggtgagcttc cgaagtacat ccacgaagag atcctccttg
    11041 ctcgggtaat atacgtagag ggttctcttg gagaccttcg cctcagccgc tatggcatca
    11101 gtgctggagg cggcgaagcc gtcgcgcaga aacaccctcc gcgcgccggc caagatctgg
    11161 tcccgcttcg ccctcgccct cggaccaagc tcctcagatg tcgcaaccac cgcacctcct
    11221 atttgagtaa actaaacagt accgtttatt atgagtagac tgactaggtt tgtcaagaat
    11281 ggttctgggg gacgggtagt gtttcaggtg aggatgaggg cgagcagggc ggcaacgagc
    11341 gtgcagagta ggtttgtcag ctcgttaccg gcgcggggtg agagggcccc gatcaggcta
    11401 tccgcgagcg ttcccaggaa cgccgctatc gctacgagga gggcggatgc aggcgtctct
    11461 agcattccga gggagagtcc cagcaaggcc gtaagaacgg ctgctccggc gcccgccagt
    11521 gtccctggaa acgagacggc cccgtcggtg cctggcggga ctttttggag ggtggagatc
    11581 aaacgcggcg tgcggctgaa gagctggcct atctcggact ctgccgtgtc tgcgaaggcg
    11641 gcgccgaggg aggcgacgaa ggctgcggca aagggctccg accctgttgc ggcaaggata
    11701 gcgcagaggg tggcgacggc gcagttggcc agggcgttgc gggcgctgcg gcgtcccccg
    11761 tgttcctggg cggtgcccgt acgttgcttg ctaccgtagc cgaacctggt caggagggag
    11821 cccccgatca cgaacagggc gaggacggcg aagccgcgtg gacccaggga ggcgtagatc
    11881 gtggccccta ccagaagtcc gccaagggcg ccgctggggc tgatcatccc aagcgcgtag
    11941 gctagggcgg cgaaagcccc ggttaccagg agggcggtca ggacctggga ggccaagtgg
    12001 tccctacggg ctcccgagcc gttctaggag ccggcgagcg tagcgctcgc actctgcagg
    12061 aatggcgtgc aggagggcgg gttcaaccat accggccttc acgccggtcc ttgtcaggcg
    12121 ctcgacgccc tgatagagga gagacccagc caggggacta ggtttgctgg gaacagcgac
    12181 gatcacaccg tagaggtgcg gcgcgccagg gtcgccgacc gcgccgacgg cgtcgatggc
    12241 gtctgggcgg tcctgcccga tggcgaagac atgcacggga ggatcttccc tgggaatccg
    12301 gggctccctg agactctccc agtgcgccgt ggtcttcgtg aaccccgctt ccagcgcggc
    12361 cctggagagg cctgaccaga cgtcgccggc caccctactg gcgagtggac tctcgtagtc
    12421 cacgaagatg cctaccttgg cttccgacac gccagggatg ttagcagagt cgagggctat
    12481 ccctccccca cctcgcgctt ctgtaagcgg cgcaagaaga catctatctc cttcacggtc
    12541 tggaggtcgc cgttcccttg ggcaacctct ctgcccttaa ggaagatgtc cctggcttcg
    12601 gcattttcat ggagctcgta gtgggcgcgg cccagcatcc tgtaggcgac agagtaatcc
    12661 ggcttgttcg cgaccgcgcg ctggaggtga tccctggcct ccctgtagcg tccctctttg
    12721 aagagctcgt tgccgagcga gtagaggacc atcgggtttc tcgggtccct ctggagcagc
    12781 ttgcggaaca tgtcagatcg ggaagcgttc atgcgtcctt tttagcatgc gggagaggga
    12841 tctgttagat tcggcccccg taccttcccg ctatctggaa aggcggcaat ggacataggg
    12901 ccgctcctgc gggagctcgc tgagggcact gcgctggagc cttttcgggc gctcatagaa
    12961 cccctacaag agcacgacaa gacgcgccgg agcgacctgg tgctgactct tagggtgtac
    13021 ttcgcgaccg gcggcaacgc gagcgaggcc gcagatcggc tgttcttgca ccgcaacagc
    13081 atgctgtacc gcctggagcg catacagaag ctgaccggtt tagacttgag agaccacagg
    13141 gtagcgcttg cgctacaact tggactgctg gctaaggaga gaggagagcg aattgcaact
    13201 gagtacccgt aaccgactgc caggcaccat caccgaggtg gtaaagggag aagcggcggc
    13261 caaagtaact ctccaggtcg gggacaatca cgtggtggcc ctcattacca gggagagcgc
    13321 cgacgagctc ggcctcgaac caggcaagca ggctaccgcc ctggtcaagg cgacggacgt
    13381 catgatcctg accgaaggcg gagatgtagc ctgaccaacg gcgcaaggca cagatagacc
    13441 ggggcttatg cccggtcttt ggtagatctc gcggtcaggc atctgacggc caggatcacg
    13501 gcgaaagcga agcccaggac gagcacggag agcgccacgg cggcgtcgag gtctctctga
    13561 aactcggcaa agatggccag tgggatggtc tgagtaacgc ccctgaagct cccggcgaag
    13621 atgatcgtcg caccgaactc tccgagcgcc cgcgcccagg ccgtgagcgc ccctgcgacg
    13681 agcgcgggta aagccagggg tattgtaact ctgaagaatc gcctccagtg gccagcccca
    13741 tcgaccatgg cagcctcctc taccgcccgg tctaccgcgc cgaaacccgt cgcggcctgc
    13801 ctgacgtaga aaggggctgc cacgaagacc tcggccatca ccacggccgc ggtcgtgaag
    13861 ctcagggtga tcccgaagac gttcaggtac tgtcccacaa ggcctagcct cccgaatgcc
    13921 agtaacagcg ctatgcctgc cgcgctcggc ggtagcacca cagggatatc tatcagggtg
    13981 ttgatcacac gactccccct gaactcagcg cgcgccaaca cgtaggccgc gggcgtccca
    14041 atcaggagca gaatcgccat cgtgatggcc gtggtcctga ggctcagaag cagggcgtcc
    14101 accgccacgg gagaggccat cgcccgccag gttcctttgt ctaccgtcca gaccacgagg
    14161 ctaagcaggg gcaagcttac gaagccgacg agcagcgcga gggaggccaa ggctaggccg
    14221 gaagcgagcg tgtcgctccc gcggggaagt ctagtagagg aaccgacttt gtcgcgtccc
    14281 ttcacgccgc tggctcgaag ttccacttct ggagcacgcc ctggccctcc tcgctcctca
    14341 ccagttcgac ccacttcttc gcgagctcgg ggctttttgc atcctcgagg gcggcgatcg
    14401 ggtagggcgc gacgatgttc aaatcgggcg ggatccgtac gatctctacc ctgtccctta
    14461 tgtccggggt gtagtcgctg gcgtagccga aggtggcgtc ggcgtcccct acgaccactc
    14521 ggttgacgga tgcccgaacg tctgactccc tcgaaaccac gttggagagg acatccttct
    14581 cgaagtcagc cccgtactcg cccttggctt tgcccagtat ctccagggca tagtcggcag
    14641 ccggcacatc tttccctgcc agcacgagct ttacatcggg cttcgcgacg tcccggaacc
    14701 gccttatgtt cgccggattg tccttcggta ccatgacgat ctcgcggttc ttcacgaaga
    14761 cctcgggctc gcccttcacc aggccttcct ttacggctgt attcatctcc tccttggccg
    14821 cggaggcgaa gacgtcagcg ggggctccct gttggatctg ggcgagcaac gtcgagctgg
    14881 actcgaaact ctgcttcact ttcacgccag ggttctgttc ttcgaaccgg ttcgccaaca
    14941 ccccgaaggc gtcgatgagc gaagaagcgg ctagaacggt cagcgtccct ccctgctgtt
    15001 tccctccacc gccgctttcc ccacctgaaa ctccgccaca accacccgcg atccccgcca
    15061 gagcaacgcc cgaaaccaac aacgcagcca acggtgtcag acggacccgc ccgtgcccgc
    15121 taatgccttc tcctttatcc agacatctgt cctgccgaca gctaatgcta gagaaaggct
    15181 gctacccggt ctttgtacaa agtgcacgaa atcctgaagc cgtgttgtga ggaaggccca
    15241 agtccttcct cacagcactc tagatgtagg ggccgacgct ttcgtcctcc gccaggcgcc
    15301 tgtacgcttc cgccacggcg aggaccttct tcacgtacca gtcggcgtgg ttgtaggagt
    15361 acagcgctct ataccagtcc cgcggcgccc cacagtcttc gaggtaacta gcggccgcgg
    15421 gtatggcgtc ttcggggtcc attacgttgg ccacaccatc gccgttgccg tccacgcccg
    15481 aagtctccca cgtcgttggc atgaactgca tcgggcccat cgcgccggcg ctggacggtc
    15541 ccatggcctg accgtggttg gactctacct tcccgacggc cgcgagtatg taccagtcgg
    15601 gcccgaaccc gtatttcgtg gccgacgccc tgtagagttc catgtaagca ttctcggagg
    15661 taggctcgac ggatgtggct actatgtctt ctcgcgcgac ctcgagctcg tgctcgctac
    15721 ccacctctcc cccgccggta gccgccgtgg tcttcaaaat acgcgccctc tccgccgctt
    15781 tgagcttttg cagacgggct tccgtccgcg aattgttggt gcccaggcgg tctatggact
    15841 ggtcgagcgc ctgctcgcgc acgcgaaggt ccgcggtcgt cgcctgctgc tcctcgatgg
    15901 cagcattgta gtcgcgccgc ttctgcgcga tctgacgttg cgtgttcctg agcgtttgcc
    15961 tggactctcg atacgcggcc agattctgtc gactatcgag gaggatgcgg gccacaacgg
    16021 gatcacctgt cccagcgacg ccctcccagg aatcgaagaa ccacccatcc agcaaggcgg
    16081 ccaggccttc gaggcttcca cccttgtagg cggcttgagc ctgagccctg tacctggctt
    16141 gcgcctcgcg gaaggccgca tcctgctcgg ctatctgtcg tttcaattgc ctggtctgtc
    16201 gtctgagatc atcgacgcgc gcccccgcat catccacccg cgattgcgcc cggtccaact
    16261 ctgcgcctac ggtggagatc tcctcgatca tgccccgcaa tgcggcgtcc tgatcactta
    16321 tcttggcttc cagcaaaggc acccgcgtgg atcgtgcgac agacatatcc tcgtccgcgg
    16381 attgcgcccg cgccggcccc tcagcacccg ccaggacaag cgcaagagac aatacgccca
    16441 aactcgacca aactcttagc tgtcctcccg acatgccgtc ccctccaccc gcgccagaaa
    16501 cctcgagata gcgcagacag ctaagtctat actaacgcaa caaaaagcgg tagatctcat
    16561 tgcaggccgg taacttccct attctatgca cacaaaggta attgtgtatt tagtgtcttg
    16621 cgtcaggtat catttcgtga agtcaggagg gacacgagtg cttggagttc ctctgtttgt
    16681 cggaagcgga tctcgacctt tccgccgcgc ttgaggccct tcactctcac gggcaattcg
    16741 agggcttcgc gcatgaggcg tgaggcttgg tggaactcat ccggggtcgc attacttctc
    16801 tggggcatgg cactcgaaga ctcgcgaggg ttgcccagca tctcgcggac gagctcctcg
    16861 gtctttctga cggagagctt ctcatccact accctttgtg ccagccgggt catctgctcc
    16921 tctgtctcca gccccaacag tgcacgggcg tgtccttcag agagctttcc gtcgcccagg
    16981 agggcctgaa tcttctccgg caagtgcacg agccgcaggg tgttggttac cgcggccctg
    17041 ctcttgccca gccttcgcgc gagccgctcc ctggaaagcc cgaacccatc catgagagcc
    17101 tggtaggcgg ccgcagtctc cagagggcca aggttctctc tctggaggtt ctctacgagc
    17161 gccagctcga tggactcgtc ctcgcctgcc tgcctaatga ggacaggcac cctctccatg
    17221 ccagcttctt tggccgccct cagcctcctc tctcctgcta tgagctcgaa gccgcccggg
    17281 gtcgggcgta cgaccagagg ctgcaatata ccgacttcct gtatggaggc ggcaagctct
    17341 cgtatccctg cctcgggaaa gctggttctc ggctggcgtg agttcgggcg gatcgccgat
    17401 acgggcaact cctcgaactt cagtccccct acgctctccc cggtagcgat gagggcggag
    17461 aggccccgcc ccaaacccct cttacccacg gttcaccact tcgtccgcaa cggccgaata
    17521 cgcctcggct cctgtgctct tcggcgcgta gagggctatg ggcatgccga agctgggagc
    17581 ttcactcaac cttacgttcc tcgggactat ggtgtggaag acgcgttctc cgaagaagga
    17641 acgtacttct tcggccacct gatgggccag gttcgtgcgt ggatcgaaca tcgtcaggag
    17701 caccccgccg acctcgagac cggggttcaa ttcctccctg accatcctga tgctctgcat
    17761 gagctgggtg agcccctcga gggcgtagta ctcgcactgt atggggatga gggcctcatc
    17821 agcggcagtc agcgcgttga gggtcaagag atccagggcc ggtggacagt cgagcaacac
    17881 cctgtcgtac gatgactgtt tctccagagc ccgcttgagt ttgaactccc gtgccagact
    17941 ggagaccagt tcgacctcag ccccgaccag gttgatggtg gccggcgcga cgtcgagccc
    18001 ttcgatctcc gtctttacag ccacgtcccg caagggtttg ccctcgagca gaacgtcgta
    18061 catgcatccg cccgggcctg gcgacacacc gagcccactt gtggcgttcc cctgcgggtc
    18121 catgtcgacg acgagaatcc tttcgccctt tcctgcgaga taagccgcca ggttgatggc
    18181 ggtcgtgctc ttgccaacac cgcctttctg gttcactatg gctacgatag tcctcaccgc
    18241 gggcatctta gcacgtcgtt tgtctgcgtt gctcagcgaa cgcctagcgg tctcttggcc
    18301 acaattccag acaggcgcgg atacggttct ggggtctccc ggatcttttc gaggattacg
    18361 aggtttcgat ctttctcacc gacctcaggg agcatgggca ccctcgtagt ctccgccacc
    18421 ttcgccccga gcgcgtcaac cgccctgttg ccctctgcaa gctcctctgg ttccaaccga
    18481 cctttcatgg cgatggcaca accaccggtc tcgaggagcg gcacacagta ttccgcgacc
    18541 actgataacc gggctactgc cctgcaagtc acgacatcat atgcgccgcg atgccctctc
    18601 gctctgccga gatcctccac cctcgtattc gcgacttcta cgccccttag ggagaggccg
    18661 cctaccgcgt gccgcaaaaa actggccttc ttgccggtcg attctacgag ggtcatggcg
    18721 agatcgggcc tcagaatctt tatcgggatg ccgggaaggc caccaccact tcccacgtcg
    18781 gccaaacgcc aggcacgaaa gagcggttcg tgcaggaagc aactcaacga atcgagaacg
    18841 tggtccatca ggatcctatc cacgtcccta gtccctatga cgttcgcaag gtcataggaa
    18901 gcgagaagcc tggcgtattc ctgcaggtgc ccccgccggt cgtggcacag gcgcagcccc
    18961 caggcagccg cctgtgcctc catcaggcca agagcagcca tagggcgttt cacgtgaaac
    19021 atctacccgc gacaggagtt tgtttgtgca aatttctgtc gtcaggcgtg tacggtggcg
    19081 atagtaatag tttgcgtttc acgtgaaacg agtttcctat gcctgagtta tgggagagac
    19141 cttgacccgc cgattatcgc cgctgccttc gctctccgtg gtgacccctg ggttctctga
    19201 gagaaagagg tgtacgacgc gccgttccga tgagttcatg ggcggcattt ccacggtccg
    19261 tcgctcgcgg acggcacggc gggccgttct gtgggccatg ccctggatcg cctccacgcg
    19321 gcgttggcgg tatccctcgg cgtcgagcac gatccttttg aagaagggtc ggtctctgaa
    19381 cgctgacacg ttgaccagat actggagtgc atcgatagtc tcgcccttct gccctataaa
    19441 gagcgctgtg ttatcagggg cgacgtccgc ggcgataaac ccgcccgcat cgtagacctc
    19501 tacacgggct tcgaagccca tagcatccaa aaccgaagaa acgagggctc taatctcgtc
    19561 gagcagctcc tgtggtgcct ccacctgttc ggactccgat tgttccccgt agacttcgga
    19621 cagggtggaa gagccagcat tttcagaggg ttccggctca ggctcgtcga ccaccacgtt
    19681 ctctgcaggc gtggtttcag gatgttcgtc ttcgcccgag gcgtgtggtg ctgaggcttg
    19741 cacgatgatg cgggcatccc tggctccgat accgaggaaa ccagaattgc cctcatcaag
    19801 cacgcggtac gaaacctgct ccgaggatag gcctagctcg agggccgcct tctcgacggc
    19861 ctcctcgatg gtagaggcgt aaaactccat actgtccgtc atttcttctt ctttctacgc
    19921 ttcctctttg cgaccttggc ggcttgtttg gcgatctcct cttgactgcg ctccgaggat
    19981 tgttctccac ccgaatttcg agcctgcccg tccgtggctt tcagctttcc gccgtcgatg
    20041 cccttcgcac catcggtggg ctttttacca ggaccgtagt tatagatcac gaagttctgg
    20101 cccagtgtta ccaggttaga ggtgatccag tacacgaaca gtccggcggg gaagttgagg
    20161 aggaatatag taaagcctat aggaagaagg cgcataagcc agcgctgctg cgggtctatg
    20221 ttcttggcgg ttatctccgt ggccgccagc atcgttaccg ccgagatgat cggcaagagg
    20281 tatgtggaat cggctacaga gaggttctgg aaccacagga tgccgccctc gtggaagctg
    20341 gggtattgcg cgggcaccgt cctatccccg aagctgtaac cgccgaactg gcggataacg
    20401 tagaagatcc cgataaatat gggcatctgg actaggatcg ggagacatcc gccgagggga
    20461 ttgacgccct gctcctgata gaccttcgcc atctcctccc gctgccgctg gggattttcc
    20521 ttgaactgcg ctcggactct gtccatgtgc ggtttcagat cctgcatggc gcgcatgctt
    20581 ctcacctgtt ttaccgtcag ggggaagagg aggctccgta cgatcaccgt cagcagcacg
    20641 atcgcgagcc accacggcgc gccaagcgtc tgatggaagt acagcagtac tgcgccgagg
    20701 acgctcacta cgggactgaa taggttctgg aaaaagtctg ctattgcgtt gagcatttga
    20761 tcctactcat tttaccgggt cgaatcctcc cttcgagaac gggtgacaac ggagcaccct
    20821 gaggacgccc atcaaaatgc ccctcggtac gccgtacttc tctatggcct gcagcgtgta
    20881 gagggagcag ctcggcgtat atctgcaaga cggggtcatc atgggggaga ggtacctgcg
    20941 gtagagtcga ataagtcccg tgctcaactt cttcggagac atggcccgct gcagtcaaac
    21001 cggccggcgc ccgacagggc cgccgaccct gcccaacgac ttgctgaact cctcctgcag
    21061 ctcctcgaac gtagcctcag cagcggccgg cctcgccgac accacgaggt ccaggttccc
    21121 ggataattcg gagatgcacg agtagaatac ctctctgagc ctccttcgca cggcgtttct
    21181 cgttaccgcg ttgccgacct tacgggagat ggagagcccc agccgcggag tgccatgttc
    21241 gttgggaaag gcgtgcacgg agaagagcct gcccctgtaa gcggtaccct gacgatagac
    21301 cctctcgaac tcgggcgagt cagtcaggcg catgcgcttg cttcgggaca acggtcagac
    21361 tgccagacgc ttgcggcccc ggcgccggcg gtcagcgacg accctgcgcc cgcccaccga
    21421 actcatgcgc ttgcggaagc cgtgcgtctt ggctctctta cggcgatttg gctgataagt
    21481 acgcttcatt gaactcctct acaggcactc cgaagtattc agtcgaccga cgagagtata
    21541 tccgaagccg gcaacgccat ccagcgatct ccagagcaag ttcgcccccc cgaacacgag
    21601 acaacctggt ccgtatcgca gaaaaacgct gcaaaacggc gtttataatg tgcacttgct
    21661 ctgggtcacc catacctcta ctcgttctgg atagcaccag tgcaggcccc gaagtcaccc
    21721 tgaggcgcag atatgtttgc ggttatccac aggctgtgga taaatctgtg gataaccatg
    21781 ttaattgcct gcaaaaatag gttttgtgtt cttgggctat agcgctacga tgaggggtgc
    21841 gctaaattcc cttctgggga tgatgcacgc acagcgaaca ccaggggaga cggaccaggc
    21901 gggcgtcggc gttcgcatag cagtctatcc caggagcggc acgccagaga tcaccgaggt
    21961 ttcggcgtcc gacccgggtt ccgccacgcg gaagttaacc aagatcgttt cggagaaggt
    22021 tagagaggcg gggggcagag ttcccgagga gtctattcgc gaggtggtgg agaacctcat
    22081 acacgcaggc taccaggggg tggttatctc cgttctcgag gacggcaacg tcgtgcgcgt
    22141 ctcggacaag ggtcctgggg tggagaacaa gagcagggcg atggagttcg gtttcagcgg
    22201 cgccgcgcct ggggcccttg gtgagatccg gggcatcgga gccgggcttg gcatcgcccg
    22261 cgcggcggcc gagaaggtcg gcgggacggt caccatcgag gacaatatcg gtggcggcac
    22321 ggtggccacc gtctctgcgg aggccaccga gactgtcaca ggggaaggag aggcggcatc
    22381 agtgccaccc ccgcagaggc ggtaccccga cggagtaccg cggatgaaca tctccgagag
    22441 gcagcagaag gcactcatca cggtactcga gtgtggcgag gtgggtcctt cgaccgtggc
    22501 tgacaggctg gagataagcg taagcacggc ctacagggat ctctcggtcc tcgaagaaca
    22561 cggccttgtc ctggctgatg agtcgggcaa gcgcctcatc tccccgctcg gcagggatct
    22621 ggtcgaggcc atcatcaatg cctgggtcag gtaagcgtgg ccggtaaggc agagcaggtc
    22681 tggacggagg tcttggatcg ggcctcagaa cacatagacg tctcgtcgct acgggtctgg
    22741 ttcgagggca tacgtcccgt agccctctac gaggatcagc tggagatatc tgtccccaac
    22801 acgttcgcca aggagtacat cgagagccgc ttcagaccgc tcctggaaga ggtgctcgac
    22861 gacgtggtag gccaggagac atccctcgtg gtcagcgtgg gaggacccgg cgtccatacc
    22921 gggaacggta ctgccgctga gggcgccgag tctgtcctga acgctcgtgc gccgagatct
    22981 ttcagggcca agtacacgtt cgacacgttc gtcattgggg ctggtaacag gttcgcccac
    23041 gcggcgacgc tcgccgtcgc agagacacct ggcctcgtct acaatccact ctttatctac
    23101 ggcggcgttg gcctggggaa gactcacctg ttgcgcgccg ttggacacta cgtggaggac
    23161 caggatccga gcatgaggat ccggtacgtt acgtgcgagc agtttacgaa cgactttata
    23221 aactccatga gggacaacgc gcccctcgag ttccagaagc gctaccgcga gaacgacgtc
    23281 ctcctggtcg acgacatcca gttcctagag aacaaggtcg agacccagga agcgttcttc
    23341 cacaccttca acgcgctcta tgaggagaac aagcaaatag tcatagcctc ggacaggcac
    23401 cccagataca tccagacgct ggagaaccgt cttgtcagcc gcttcgagtg gggccttgtg
    23461 acggacatcc agccgcccga cctggagacc aggatcgcca tcctgcgcaa gaaagccctt
    23521 atggaccacc tggaggtcga cgacgatgtg ctcaccttta tcgcctcgaa agtctccact
    23581 aacatcaggg agctcgaagg ggccctggtc cgtattctgg cctacgcctc gctctacggc
    23641 cggcaagtca gcgccgacct cgccgaggag gtcctcaggg atatactgcc ggaccaggca
    23701 taccgtgaga tccccataga actcatccag cacgaggtct gccgatactt cggtatcgcg
    23761 aaagccgacc tcgtaggcca gagccgctca aaaggtttcg cctaccccag acaggtcgcc
    23821 atgtacctct cccgggaact cacagacgag tcactcccca agatagggaa ggccttcggc
    23881 ggacgcgacc attccaccgt tatgcacgcc acggcaaaga tagccaacct cataaacagc
    23941 gaccgcgacg tcttctccca aatacatgag atcacccaac aggtcaagag taaaaggtga
    24001 c
//
LOCUS       DASTKM010000076         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_544658, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000076 DASTKM010000000
VERSION     DASTKM010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544658"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFQ10_03045"
     CDS             complement(<1..302)
                     /locus_tag="VFQ10_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9964348.1"
                     /translation="MELGGLHHMTAVTTDAAENVAFYTDVLGLRLVKKTVNQDDVSAY
                     HLFYGDEVGNPGTEITFFDWPHLRVAPNHPGAGEVSTTELRVPDNAALDWWAGRFQ"
     gene            complement(325..981)
                     /locus_tag="VFQ10_03050"
     CDS             complement(325..981)
                     /locus_tag="VFQ10_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9964349.1"
                     /translation="MGRERTQGLGWSLDHLGRPSGWEAERSDAARNRRVILRTARTLF
                     EERGVTCVTMEEIARASGVGKGTLYRRFPHKGFLCQALLDEPTRLLQEQTLEILREPG
                     TPAVEKLDAFLNRLVAFTAENLDLLYGGNEPLCGADRLERFSDPAYDWRRSTVLGLLR
                     AAVNQGDVDAGLDLEYLATALLAPLDVDLFYYQHRVQGLPTWRIGSGVRAFVPALEKA
                     "
     gene            1162..2184
                     /locus_tag="VFQ10_03055"
     CDS             1162..2184
                     /locus_tag="VFQ10_03055"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET9964350.1"
                     /translation="MRSGTHTFLQTTRVHFGAGALENLEEEARSYGRAFVITGRSLNE
                     RTGLISRVEAMLGGRHAGTFAGIGQHTPGGAVEQAAVEAELAAADLLVSVGGGSVIDG
                     TKAVARELGYPQQVAIPTTLSGAEWANRAGVTDERSGRKRGFADPKTVPRVVVLDPEA
                     TLFTPEKLWLSTGIRALDHAVEGFLFGGDHPVTDVTGLEGARRLMEYLPLSRSIPKDL
                     EVRQELQLAAWLSYFGPANTPMGLSHELGRRIGASYEVPHGITSCIILAPSLQVVKER
                     VQDDRWTMLSQALGGEPPERVSSLVEKLGLPGRLREVGVPEEDLEDISGEYGDGEALA
                     ILKAAY"
BASE COUNT          448 a          743 c          756 g          424 t
ORIGIN      
        1 tcctggaatc gcccggccca ccagtcaagc gcggcgttat ccgggacgcg caactcggta
       61 gtcgagacct cgccagcacc gggatggttg ggggctacgc gcagatgggg ccagtcgaag
      121 aaggtgatct cggtcccagg atttccaacc tcgtctccgt aaaataggtg gtaggcggag
      181 acgtcgtcct gattcacggt ctttttgacg agcctcagcc cgagcacgtc ggtgtagaat
      241 gccacgtttt ccgcggcgtc cgtcgtgacc gccgtcatgt ggtgcaggcc gccaagttcc
      301 atctctatcc tccgatcggt cgtctcacgc cttctccagc gcgggtacaa aggcgcgcac
      361 accgctgcct atgcgccagg tcggtagccc ctgtaccctg tgctggtagt agaacaggtc
      421 cacgtcgagc ggtgcgagca gcgccgttgc aaggtactcg aggtcgagcc cggcatctac
      481 gtctccctgg ttgaccgcgg cccgcaagag cccgaggacg gtcgatcggc gccagtcgta
      541 cgctggatcc gagaagcgct ccagacggtc agccccgcac aacggctcgt tgccgccgta
      601 gagcagatcc agattctccg cggtaaaggc cacgagacgg ttcaagaacg cgtcgagctt
      661 ctcgacggcc ggcgtccctg gttcgcgcag gatctccagc gtctgctcct ggagaagcct
      721 ggtaggctcg tcgagaagcg cctggcacag gaaacccttg tgtggaaagc gccggtagag
      781 cgtacccttc ccaacacccg aggccctggc gatctcttcc atggtgacgc acgtgacgcc
      841 gcgctcctcg aacagcgtcc gcgccgtacg caggatcaca cgcctgttgc gcgccgcatc
      901 gctgcgctca gcctcccagc cagagggacg ccccaggtga tccaaactcc accccaaccc
      961 ctgtgtgcgt tcacgcccca cgactatcac tctccatcga gaccttgaca ccggcagtct
     1021 accgcgctac cctcgcactt taaagtggac tcacgtccgt ttttgtcgtg taacctgaaa
     1081 acaggttgtg ggagcatacc ggtaagcgag tgccgcatcg ccgcggtcgc ctggagttgc
     1141 gcgtagcggt acgataggag cgtgagaagc ggtacccaca ccttccttca gaccacgcgc
     1201 gttcatttcg gcgcgggtgc tctggagaac ttggaagaag aggcccggtc gtacggccga
     1261 gccttcgtca tcaccgggcg cagcctgaac gaaaggaccg gtctcatcag ccgtgtcgag
     1321 gcgatgctcg gcggcaggca cgcggggacc ttcgccggta tcgggcagca tacccccggt
     1381 ggggcggtgg agcaggccgc cgtcgaggcg gagttggcgg cggccgatct cctggtcagc
     1441 gtcggtgggg gaagcgtaat agacggcacc aaggcggtgg cgcgggagct aggatatcct
     1501 cagcaggtgg cgatcccgac caccctctct ggcgcggagt gggccaacag ggcaggggtc
     1561 acggacgaga gatccggtag gaagagaggc tttgccgacc cgaagaccgt tccgagggtc
     1621 gttgtactcg atcctgaggc taccctcttc acgccagaga agctctggct ctcgactggc
     1681 ataagggccc tggatcacgc ggtcgaagga tttctcttcg gaggcgacca tcccgtcacc
     1741 gatgtaaccg gtctcgaagg agctcggcgc ctgatggagt atctgccgct ttcgagaagc
     1801 atacccaaag acctggaggt gcgccaggag ttacagctcg cggcctggct ctcgtatttc
     1861 ggaccggcga acacgccgat gggtctctcg cacgagctgg ggcgcaggat cggggccagc
     1921 tacgaagtcc cgcacggcat tacctcctgt atcatcctcg caccaagcct gcaggtcgtg
     1981 aaagagcgcg tacaggacga ccgctggacc atgctctccc aggctctagg tggagaaccg
     2041 cccgagcgcg tgtcatcgct ggtcgagaaa ctcggattac ccggccgcct tcgcgaggtc
     2101 ggggtgcctg aggaagacct ggaagatatc tccggggagt acggcgacgg ggaagcgctg
     2161 gcgatcctga aagctgcata ctaggatgag cgggcgatga tcgccgcgag actccagagg
     2221 gatgatgagg taaggatagt ctcacctgcc gtcagcctcg ggttcatacc agaagatcag
     2281 cagctgacag ccagggagcg gctcgaaggt ctaggcctcc gttgttcgtt ctccacaaac
     2341 gccgaggtga ttgatcgctt cgattcctcc c
//
LOCUS       DASTKM010000077         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_897395, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000077 DASTKM010000000
VERSION     DASTKM010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897395"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFQ10_03060"
     CDS             complement(<1..252)
                     /locus_tag="VFQ10_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964351.1"
                     /translation="MKARELLSGLGLPGRDLYHLPDSGKRFPDGAQYRVEIPSVEGPR
                     VLEAVIEEADRREVQIHRVSQGSGIMLLTDAEILEMCLMA"
     gene            complement(249..983)
                     /locus_tag="VFQ10_03065"
     CDS             complement(249..983)
                     /locus_tag="VFQ10_03065"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET9964352.1"
                     /translation="MEQSRRTRLRDRAANQLLEMVISDGLEPGERLPPERELCAHLGV
                     SRTVVREALNLLEARGLISIEHGRGAVVSGGNPRAVRETLGLLLRVQPKTLWELLEMR
                     KILEVEVAGLAAERAGPDDLTAMRLQLDRMRDSIEVPEGYVDADVEFHALLARATRNE
                     VLLTMLEPVVDLLRASRRVSAARPGNALRALGEHERILRRVEAKDAGRAREEMRTHLA
                     KTAEDIEAAIGEGVLDAGELLEKEGV"
     gene            complement(1043..1795)
                     /locus_tag="VFQ10_03070"
     CDS             complement(1043..1795)
                     /locus_tag="VFQ10_03070"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET9964353.1"
                     /translation="MREGLTRRQGGEEDFRWRGEDVSRIEGFSDAVFAFAVTLLVVSL
                     EVPKTFDELLATMRGFFAFAICFALLLTVWYDHYKFFRRYGLKDTPTRWLNSTLLFLM
                     LLYVYPLKFLFTMLIDEWLGFGNSEMIEASQIPLLLVIYGAGFVAIQLVFFLMYLRAY
                     SLRTALELDSQELSVTREEMQGFFLNVLVGLVSVAIAALGGVGATSWAGLVYLLVFPL
                     QTINARVMGSRRRKESSSRTQARGEDADSEET"
     gene            1956..>2415
                     /locus_tag="VFQ10_03075"
     CDS             1956..>2415
                     /locus_tag="VFQ10_03075"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9964354.1"
                     /translation="MILPGRERVEFRELDRPEPGHGQVLVKMKASGLCGSDLRAIYHE
                     HTGSGAERYQNVVAGHEPSGQVEAVGPGVSGFEPGDRVVVYHIQGCGRCEECAKGFMI
                     GCTSQERSAYGWQRDGGHADYLLAEARTLLHLPEGLTYTDGALVACGFGTA"
BASE COUNT          456 a          808 c          737 g          414 t
ORIGIN      
        1 cgccatcagg cacatctcca ggatctcggc gtcagtgagc agcatgatcc ccgacccttg
       61 agagacgcgg tggatctgga cttctcgtct gtcggcctcc tcgatgaccg cctccagcac
      121 ccgcggtccc tcgacgctgg ggatctcgac cctgtactgc gccccgtcag ggaaacgctt
      181 ccccgaatcg ggcagatggt agaggtcgcg tccaggcaac cccagtcctg ataacagctc
      241 cctggccttc ataccccctc cttctctagg agctctccgg cgtcgagtac cccttcaccg
      301 atggcagcct cgatgtcctc agccgtcttc gcgaggtggg ttcgcatctc ctcccgcgct
      361 ctcccggcgt cctttgcctc cacccggcga agaatccgct cgtgctcccc tagggcccgc
      421 agcgcgttgc cgggcctggc ggcgctcact cgtctgctgg cccgcagcag gtcgactacg
      481 ggctcgagca tggtaagcaa gacctcgttg cgggtcgctc gcgccagcag agcgtggaac
      541 tcgacgtcgg cgtcgacgta gccttcgggc acctcgatgg agtcccgcat cctgtcgagc
      601 tggagccgca tggccgttag gtcatcgggg ccggcacgct ccgccgcgag acctgcgacc
      661 tcgacctcaa ggatcttgcg catctccaga agctcccaca acgtcttcgg ttgtacgcgc
      721 aggagcagac ctagggtctc acgcacggcc cgcggattgc cgccgctgac caccgcgccg
      781 cggccgtgct cgatgctgat gaggccgcgg gcctcgagca agttcagagc ctccctcacc
      841 accgtcctgc taaccccgag atgcgcacac agctcacgct ccggcggaag acgctctccc
      901 ggctccagcc cgtccgatat caccatctcc agcaactgat tcgctgcccg gtccctgagc
      961 ctggttcgtc gtgactgctc catcacccta aagcattgtc ataccaatca ggatgcctgt
     1021 caaaccctgg ccgtccgggg ctctacgttt cttcgctgtc cgcgtcttct cctctggcct
     1081 gagtgcggga cgagctctcc ttccttctgc gggagcccat aacgcgggcg ttgatcgtct
     1141 gcagagggaa gaccagcagg tagaccaatc ctgcccagga ggtcgccccg acacctccga
     1201 gggcggctat ggcaaccgag accagcccga ccaagacgtt gaggaagaag ccctgcatct
     1261 cctcgcgcgt cactgacagc tcctgcgagt cgagctccag ggcggtgcgc agtgagtagg
     1321 cccgcaggta catcagaaag aacacgagct ggatggcgac gaagcctgca ccgtagatga
     1381 ccaacaagag cggtatctgg gaagcctcta tcatctcgct gttgccgaaa cccaaccact
     1441 cgtctatcag catggtgaag agaaacttca gggggtagac gtacaggagc atgaggaaga
     1501 gcagcgtcga gttcagccac cgggtagggg tgtccttcag gccgtagcgt ctgaagaact
     1561 tgtagtggtc gtaccacacg gtgaggagca gcgcgaagca gatcgcgaac gcgaagaaac
     1621 cccgcatggt cgcgagcagc tcgtcgaagg tcttggggac ctccagggac accaccagga
     1681 gcgttaccgc gaaggcgaag acggcgtcgg agaacccttc gatgcgcgag acatcctccc
     1741 cgcgccagcg gaagtcctcc tcgccgccct gcctccgcgt caacccttcg cgcaccacta
     1801 cccattcacc ccgctaggcc tccgccggtt tgtagagtgc ccatgataga ggagctatcg
     1861 cagtacgcac agtcgtgcat cttgacggga gacgatctca taacccatga taagaagagg
     1921 acgtagaggc actggaggcg atctacttgc gaggggtgat cctacccgga cgtgaacggg
     1981 tggagttcag agagttagac cgccccgagc cgggacacgg acaggtactc gtaaagatga
     2041 aggcctcggg gctctgcggg agcgaccttc gcgccatcta ccacgagcac acgggctcgg
     2101 gggctgagcg ctaccagaac gtcgtcgcgg gccacgaacc atcagggcag gtcgaggccg
     2161 tgggacccgg agtaagcggg ttcgagccgg gcgacagggt cgtcgtctac cacattcagg
     2221 gctgtgggcg ctgtgaggag tgcgcaaagg ggttcatgat cgggtgcaca tctcaggagc
     2281 ggtcggccta cggctggcag cgcgacggcg gccacgccga ctacctgctg gccgaggcaa
     2341 gaaccctgct acacctgccc gaagggctga cctacacaga cggcgccctc gtggcctgcg
     2401 ggttcgggac cgcct
//
LOCUS       DASTKM010000078         7957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_193171, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000078 DASTKM010000000
VERSION     DASTKM010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193171"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2003)
                     /locus_tag="VFQ10_03080"
     CDS             complement(<1..2003)
                     /locus_tag="VFQ10_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964355.1"
                     /translation="MLHDSYLDQTSGPIGRVVTSEESPATAHEFFFWTAETEAAQNLN
                     IGHIVAAESEDATAVAVLDDPRRYSDLQSFLDDFYAYDGDPSLEALSERVEILVFRAR
                     VLATKHRDERKKSKRPVRTGPVFFATNTAIEFALGTEEFSGHSIPMLLHENGNEKNGS
                     PQRTPLYVDEEYLLGPEAGHLNITGMSGLSTKTSHALFTIASTFQTVEDKKVAALMFN
                     VKGADLLFLDKPVEVSAEDDPELAERYKRAGQRGLPPEDREMYESLGIEVRPFENLRI
                     FAPLTFGGDPQASTVYPADIPAKKLNTRRRAWGEDSCVYPIVWDLEDVLPHAGRIFDP
                     TDYDDKFKGFVEYLRAGQVRTMRRFHAEIDEAFAYFEESESSYWNGHHQATVAKVRNR
                     FGVLPSKCGGLLVDGEVEYGDLPQVDGSFEDREMRVVDISQLSGVPQDLVVTKVINSV
                     WEMAEQNRLGVDKLIIFVDELNKYAPSGSRTSSLKDTLVDIAARGRHLHLTLFGAQQF
                     RSRVDDEVVGNAATSLYGRIGDEELTNSSYRSFSGTTREELLQLEKGRLLLRHAHYAV
                     PIFGTFPRPMVLMGKQGTDIFGEQEQDPASSVLAVMRKLTAKPPAIIDIRSEIEGVPE
                     EQVYEALGAVQAAHRTGNGAADPYKNFRWNLKRPARTGSSRRDA"
     gene            complement(2004..3125)
                     /locus_tag="VFQ10_03085"
     CDS             complement(2004..3125)
                     /locus_tag="VFQ10_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964356.1"
                     /translation="MVGDFVEKLSQAIEGAGRRGIHVRAGSCDLEAQQFDRHLPEGEE
                     PLPTGRPHHLESPVGSRDLGDHKGISRLCYFLDGVQSSREIGRIGMSPVIVSTVAATI
                     VNRCGRRFSRMPLESPPIVVQAVILPRSAGDPGVDAFWDLLLAAGFSELDPEEVPSSP
                     HLVLDSAGYIAEADPSDYVGMRERARGRVRSLRERLEGGLLRRWEGDDRVLEDSEAWI
                     AVDGQLKDIRESNRRAIGLIKSVARPEFVGRDVGMLLDLEPGKRTTSFIPDWQLRRDQ
                     GERRTSWYLRMWPPQRGADALGSLMRVEAPRDTKPEQVDEISRWILAERAPLAKPDPR
                     WPAMIYPIQYVEKILKPLVQGPERAYARLERQLASNGRN"
     gene            complement(3174..3758)
                     /locus_tag="VFQ10_03090"
     CDS             complement(3174..3758)
                     /locus_tag="VFQ10_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964357.1"
                     /translation="MTEEKGKGLDLPERVRKSLAGAVAQRGVQARVISTQGRESMRPP
                     QGHIIIAAGESEVEAVRGPARIVRRLEFYKTFYGPVVRLALSVYPMGGEPLSAGTALN
                     VSQVSGDAALTGLGRQKTIYIHFYSATDDDLTYAFSKEIPNAPEQRGETKKVLKMARD
                     AYTQTPEERRSFSAAVGLAERQFELPVPEPDEER"
     gene            complement(3755..5104)
                     /locus_tag="VFQ10_03095"
     CDS             complement(3755..5104)
                     /locus_tag="VFQ10_03095"
                     /inference="COORDINATES: protein motif:HMM:NF005034.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9964358.1"
                     /translation="MDLRERVDGSREELLGELNEFLRMPSISAREDENGDFRNCARWV
                     AGKLEDAGAEASIMETEGYPVVYAEVGSGESTLLSYGHYDVQPPEPLDLWESDPFEPT
                     IRGGNLFARGVADDKGDVLARIQALRLYTEEHGPLPFKLKFLIEGEEEIGSPNLASFV
                     RSNADLLSAEACLWEGSMKDEAGRPMIFCGTKGMAYVELRARGASHDLHSMYGGIAPN
                     PVWRLIQALRTIKDENGEITLDGLHALAEEPSEKDLEAISRILFDDAALRESWGVESL
                     DRELSGQDALQEMLLRPTANIAGIQSGYTGPGSKTIVPSEAFVKMDFRLVAGQSPGSV
                     LELIRAHLRERGFEDIEVVNLHGVEPAKTPVDSPVVDIAIQTWQDLGEYEAIVYPTIG
                     GSGPTSLLVTDLGIPTIMTGSVANTGSRLHSPNEWVRLDDYFEAINYFARFFERYAL"
     gene            5233..5748
                     /locus_tag="VFQ10_03100"
     CDS             5233..5748
                     /locus_tag="VFQ10_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964359.1"
                     /translation="MEKKPDRDLESRRRGNHHRWDSDDFFGLDVEKMSPKAQAVTGIV
                     TGGLILLAAALLIAFTNLWWLIFIFGWAVFPAFGVFARGIAGLVDTRSELPATNARER
                     ELLEALQSRGELTPAQAAMETSLTVNEADEMLKDLAAKGHLDVRVRGGGLFYGLWETD
                     SEAGRRRIGDE"
     gene            5843..6583
                     /locus_tag="VFQ10_03105"
     CDS             5843..6583
                     /locus_tag="VFQ10_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964360.1"
                     /translation="MGERRIPETLEEAYRLRDTADSLQERDFYQEQVYHLRREEYRSR
                     GIRTEISSRRRERQSAIEFSREMFFLEVAEISRHGPGTRETRAAQQEARRYATRLDTH
                     AFLKQLASPHSGAVELYRRNYLELLRMGKSPSEAYYLSRVIGLAGEGEFRHALQSVRE
                     TGYPARGGVSYAVERAAIQIRDLPGGMILILLTFFASLFAFVWATQTWDNAEAGTIFF
                     VSLGLIAAGALMLISAIFIVTFIRSINK"
     gene            complement(7371..7925)
                     /locus_tag="VFQ10_03110"
     CDS             complement(7371..7925)
                     /locus_tag="VFQ10_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9964361.1"
                     /translation="MRTRMVLHEKGIDFEVHEVDLSNKSEEFLSVSPYGKVPVLSVNG
                     TSLYESNIVNEYLDEVYDSPKLMPENPEERASVRSWMAFADDYFFPSVYRVRMGLQRG
                     YSDDEIQEAKGKLYDALSRLERQLEGREYLVGEYTLADIAHAGNFHRLRVMAKSGEVG
                     LEKYPRVMVWIERVESRESYEASM"
BASE COUNT         1413 a         2420 c         2426 g         1698 t
ORIGIN      
        1 gcgtcgcggc ggctcgaccc agtcctcgcg gggcgtttga ggttccagcg gaagttcttg
       61 tatgggtccg ccgcgccgtt gcccgtgcgg tgggctgcct gcacggcgcc gagggcttcg
      121 tagacctgct cttctggcac gccctctatc tcggatcgga tgtcgatgat ggctggaggt
      181 ttggctgtga gtttgcgcat gacggcgagt acggaggagg cgggatcctg ctcttgctct
      241 ccgaagatat ccgtgccttg cttgcccatg agaaccattg gtcttggaaa ggtgccgaaa
      301 atcggcaccg catagtgcgc gtggcgtagc agaaggcgtc ccttctctag ctggagtaac
      361 tcttcacggg ttgtccctga aaaggagcgg tagctcgagt tggttagttc ctcgtctcct
      421 atgcgaccgt agaggcttgt ggcggcgttg ccaacgacct cgtcgtcgac cctgctcctg
      481 aactgctgtg ctccgaagag cgtgaggtgg aggtgccgac ctcgcgcggc gatatccacg
      541 agcgtgtcct tgagagagga ggtgcgcgaa ccggatggcg cgtacttgtt gagctcgtca
      601 acgaaaatga taagcttgtc caccccgagt ctattttgct cggccatctc ccacacgctg
      661 tttatgacct tggtgacgac gagatcctgc gggacgccgg agagctgcga gatgtccaca
      721 acgcgcatct cgcggtcctc gaatgatccg tcgacctgcg gtaggtcacc gtactcgacc
      781 tcgccatcca cgaggaggcc gccgcacttg ctcggcagca cgccaaagcg gttgcggacc
      841 ttggcgaccg tagcctggtg gtgtccgttc cagtaggaac tctcgctctc ctcgaagtag
      901 gcgaaagcct cgtcgatctc tgcatgaaat cggcgcatag tccggacctg cccagcccgc
      961 aagtactcaa cgaagccttt gaacttgtcg tcgtagtccg tcgggtcgaa gatacgtcct
     1021 gcatggggca gcacgtcctc taggtcccac actatggggt agacgcagct atcctcaccc
     1081 cacgcccgcc gacgcgtgtt cagttttttt gccggtatgt ctgctggata aactgtgctg
     1141 gcctgcggat cgccaccaaa agtgagtggg gcgaagatcc tgaggttctc gaatggcctt
     1201 acttcgatac caagggactc gtacatctcc cgatcctcgg gcggcaatcc tcgctgcccc
     1261 gcccgcttgt aacgctcagc cagctcggga tcgtcctccg cgcttacctc gaccggcttg
     1321 tccaggaaaa ggaggtcggc gcctttcacg ttgaacatca gggcagcgac cttcttgtcc
     1381 tcgacggtct ggaacgtgct agctatggta aagagggcgt ggctggtctt ggtcgagagc
     1441 ccgctcatgc ccgtgatgtt gagatggcct gcttcaggcc cgagaaggta ttcctcatcc
     1501 acgtagaggg gcgtgcgctg aggacttcca ttcttctcgt tgccgttctc gtgcaggagc
     1561 atcgggatgc tgtgtcccga gaactcctcg gttccgaggg cgaactcgat ggccgtgttc
     1621 gtggcgaaga acacggggcc ggtgcgtacg gggcgtttgg acttcttgcg ctcgtcgcgg
     1681 tgtttggtgg cgaggacgcg ggcgcggaag accagtatct ccacccgctc ggagagcgct
     1741 tcgagcgaag ggtcgccgtc gtaggcgtag aagtcgtcga ggaagctctg caggtcggag
     1801 tagcggcgcg ggtcgtcgag gacggcgacg gccgtggcgt cctcggactc ggcggcgacg
     1861 atgtgcccga tgttcaggtt ctgggcggct tcggtctctg cggtccagaa aaagaactcg
     1921 tgggcagtgg ccgggctctc ctcgctggta acgacccggc ctatgggacc cgaggtctga
     1981 tccagataac tatcgtgcag catctagttc ctcccgttcg atgctagttg gcgctccagc
     2041 cgtgcgtagg cccgctccgg gccctgcacc agcggtttca ggatcttctc tacgtactgg
     2101 atcgggtaga tcatggccgg ccacctcgga tcaggcttcg ccaggggcgc ccgctcggcg
     2161 aggatccaac gcgatatctc gtcgacctgt tctggtttgg tgtcgcgcgg cgcttcgacg
     2221 cgcatgaggg agccgagggc gtcggcgccg cgctgcggcg gccacatcct gaggtaccag
     2281 gaggtgcgcc gctcgccctg gtcgcgacga agctgccagt ctgggatgaa gctcgtcgtc
     2341 cgcttccccg gttccaggtc cagcagcatc ccaacgtccc ggccaacaaa ctcggggcgg
     2401 gcaacgctct ttataaggcc tatggcgcgc ctgttcgact ccctgatgtc cttgagttgt
     2461 ccgtccactg cgatccaagc ttcggagtcc tcgagtaccc tgtcatcacc ctcccaccgc
     2521 ctgagcagcc ctccctccaa ccgctccctt agcgacctga cccttccgcg cgccctctct
     2581 ctcatgccta catagtcgga cgggtccgcc tcggctatat accccgcgga gtcgaggacg
     2641 aggtgggggg aggaggggac ctcctcaggg tcgagttcgg agaatcctgc cgcgagaagc
     2701 agatcccaga aagcatcgac gccaggatcg ccggcgctgc ggggtaagat cacggcctgc
     2761 actactatgg gtgggctctc cagcggcatc cgcgagaacc gcctcccgca gcggttgacg
     2821 atcgtggccg ccaccgtgga tacgatcacg ggagacatcc ctatgcgccc gatctcacgc
     2881 gagctctgca ccccatccag gaaatagcaa agccgcgaga tgcctttgtg gtcgccgagg
     2941 tccctggatc ccacagggga ctccaggtgg tgcgggcgcc cggtcggcaa aggctcttct
     3001 ccctcaggga gatgacggtc gaactgctgg gcctccagat cgcatgaacc cgctctcacg
     3061 tggatgcctc tgcgcccagc cccttcgatg gcctggctga gtttctctac aaagtcgcct
     3121 accacgcttc ccgcttgcat aggtggcagt atacgcgggc tcgccgccga agactatcgt
     3181 tcttcgtcag gttcaggtac aggaagctcg aactgtcttt cggcgagccc gacggccgcc
     3241 gaaaagctcc tgcgctcttc cggcgtctgg gtataggcgt cccgggccat cttgaggacc
     3301 ttcttcgtct cgccgcgttg ctcgggggcg ttggggatct ccttggaaaa tgcgtacgtg
     3361 aggtcgtcgt cagtggcgga gtagaagtgt atgtagatgg tcttctggcg gccgaggcca
     3421 gtgagggcgg cgtcaccgct tacctgggag acgttgaggg cggtgccggc ggagagcggt
     3481 tcgcctccca tcgggtagac gctgagggcg aggcgaacga cgggcccgta gaacgttttg
     3541 tagaactcaa gccggcgtac gatgcgggca gggccgcgga ccgcttccac ctcactctct
     3601 cctgcagcga tgattatgtg tccttgcggg ggacgcatcg actcgcggcc ctgcgtggag
     3661 attacacgcg cctgcacgcc tctttgggcg actgccccgg cgaggctctt cctgacacgc
     3721 tcggggaggt cgaggccctt gcctttctcc tcggtcaaag ggcgtacctc tcgaagaagc
     3781 gggcgaagta gtttatggcc tcgaagtagt cgtccaggcg gacccactcg ttaggggagt
     3841 gaagccggct ccctgtgttc gccacgcttc cggtcatgat ggtcggtatc ccgaggtcgg
     3901 tgaccaggag cgaggtcgga ccgctccccc cgatggtcgg atacacgata gcctcgtatt
     3961 cgcccagatc ctgccacgtc tgtatcgcga tgtcgacgac gggtgagtcg acgggcgtct
     4021 tcgccggctc gacgccgtga aggttgacca cctcgatatc ctcgaagccg cgctccctga
     4081 ggtgggcccg gatcaactcc agtacagaac cggggctttg gccggcgacg agcctgaaat
     4141 ccatcttcac gaacgcctcc gaaggtacga tggtcttgga gcctggaccg gtgtagcctg
     4201 actgtatgcc cgcgatgttc gcggtcgggc gcagcagcat ctcctgcagc gcgtcctggc
     4261 cggaaagctc ccggtcgagc gactccacac cccaggactc ccgcagcgct gcgtcgtcga
     4321 agaggatgcg gcttatggct tccaggtcct tctccgaggg ttcttcggcg agggcgtgga
     4381 ggccgtccag tgtgatctca ccgttctcgt ccttgatggt cctcagggcc tggatgaggc
     4441 gccagacagg gttcggggct atgccgccgt acatgctgtg caggtcgtgg gaggcgccgc
     4501 gggcgcgtag ttccacatag gccatccctt tggtgccgca gaagatcatg ggtcgccccg
     4561 cctcgtcttt catggagcct tcccagaggc aggcttcggc ggagaggaga tcggcgttgg
     4621 accggacgaa ggacgccagg ttggggctgc cgatctcctc ctcgccctcg atgaggaact
     4681 tgagtttgaa gggcagtggg ccgtgctcct ctgtatacag acgcaacgcc tgtatacggg
     4741 cgagcacgtc ccccttgtcg tcagcgacgc cgcgggcgaa gaggttcccg cccctgatcg
     4801 tcggctcgaa cgggtcgctc tcccagagat ccaggggctc gggcggctgc acgtcgtagt
     4861 ggccgtagga gaggagtgtc gattcgccag acccgacctc cgcatagacc acaggatatc
     4921 cttcggtctc catgatgctg gcctcggcac cggcgtcctc cagcttcccc gctacccacc
     4981 gtgcgcagtt gcggaagtct ccgttctcgt cctcgcgggc ggagatcgaa ggcatccgaa
     5041 ggaactcgtt caactcccct agtaactctt ctctcgaacc gtccacgcgc tcacgcaaat
     5101 ccatccgcca gacctccttt gtgactgcga tgaggttagc actttcgcgt ccagccttcg
     5161 cgtcctcgtt gcgtatcttc tggcatggtc catcagggga gttgctgata tattgagacc
     5221 cgaggtaaac ctgtggagaa gaaaccggat cgtgacctgg agtcccgccg cagaggcaac
     5281 caccacaggt gggattctga cgacttcttc ggcctggatg tagagaagat gagcccgaag
     5341 gcccaggccg taacggggat agtgacgggc ggtttgatcc tcctcgccgc cgcactcctg
     5401 atagctttta ccaacttgtg gtggctgatc ttcatcttcg ggtgggcagt cttcccagcc
     5461 ttcggcgtct tcgccagggg tatcgctggc ctcgtcgata cccgttcgga gcttccggcg
     5521 acgaacgcaa gggagcgcga gctactggaa gccctacaga gtaggggtga gttgacgcct
     5581 gcacaggctg ccatggagac ctcgctcacg gtaaatgagg ctgacgagat gctcaaggat
     5641 ctcgccgcca agggccacct cgacgtcagg gtgcgtgggg gagggctatt ctacggcctg
     5701 tgggagactg attccgaggc cggcagaagg aggataggag acgagtagac atgtttctgt
     5761 cgcaactcgc tcatggtatc attcaggacc cacaaggggt tttcggcgcg tgagatctgg
     5821 agtatagggt aaacactggt tcatgggaga acgtaggata cctgagacgc tcgaggaggc
     5881 ttaccgcctg cgggatacgg ccgactcgtt gcaggagaga gacttctacc aggagcaggt
     5941 ctaccatctg aggcgcgagg aataccgctc gcgcggcata aggacggaga tctcctcccg
     6001 gcgccgcgag cgccagagcg ccatagagtt ctcccgcgag atgttctttc tggaggtcgc
     6061 cgagatctcc cgccacggcc cagggacccg cgagacccgc gccgcccagc aagaggcccg
     6121 caggtacgcc acgcgcctgg acacccacgc cttcctgaag cagctcgcct cacctcattc
     6181 aggcgccgtg gagctgtaca ggcgcaacta cctggagcta ctcaggatgg gcaagtctcc
     6241 ttccgaagcc tactacctct cgcgggttat cgggctggcg ggggagggag agttcaggca
     6301 tgccctccag agcgtgcgtg agaccggcta tccggcccgg ggaggcgtgt cctacgccgt
     6361 ggagcgggcg gcgatccaga taagggacct gcctgggggc atgatcctga tcctcctaac
     6421 cttcttcgcc tcactgttcg ccttcgtgtg ggctacccag acctgggata atgcagaggc
     6481 aggcacgatc ttcttcgtct ctttgggcct tatcgccgcc ggggcgctga tgttgatctc
     6541 agcgatcttt atcgttacct ttatcaggtc gataaacaag tagtcccttt acatcggggc
     6601 gcgaacacat cctgcggggg gcatagtaac gccacattca cccgtacccg acacactgat
     6661 tcctgcactt gcgataactg tcaatctccc acttctcaac tgtcaataac tgtcaaatta
     6721 actgtcaggt catcactcac tcccctttcg ctgcttccac tgttctagct tggcgttact
     6781 gccgatgtgc tcggtcttgc gctttccatc ctctagtatg gtgtgcgagt gtcgaagcta
     6841 tggtgcataa tgagactatg ggtaaggtaa tcctagctat agtcgtcata gctgtcatag
     6901 ccgcagtggt gtggttcgtg ctgactcgta ggcgggggta acggcagagc acgagcttgt
     6961 agcagcttgt gacatgtacg tggctcaggt atacgtctcg tgctcaccat gctctctgtt
     7021 tcctacactc gcgctaactg tcaacacccg cttttcaact gtcaaattaa ctgtcaatcc
     7081 ctaccgcgta tagtcctccc tttctccgat aacttgccct cggggtcgtc agccttaccg
     7141 agataggttt tcctttgctc gccgtcctcg tggtatcgga aataccaata aggcccacgc
     7201 ccccgcagca ctgaaggtta acccttgcca aagccagtca gggaggttca tctgataggt
     7261 attctacaag gtgggtgtta gcggtaacag agcccgtggt gctgcaaagg atactctgtg
     7321 ggacagctac catcgagcat tagcggtact ggcccgaccc agatggtttc ctacatggat
     7381 gcctcgtagc tctccctact ctccacccgc tctatccaaa ccatcactct agggtacttc
     7441 tccaagccaa cctcaccgct cttggccatc actcgtagac ggtggaagtt gccggcatga
     7501 gcgatgtctg ccaaggtgta ctcccctact agatactccc taccctctaa ttgacgctct
     7561 aggcgtgaga gcgcatcgta gagcttcccc ttagcctctt gaatctcatc atctgagtac
     7621 cctcgctgca gccccatgcg aaccctatag acagaaggga agaagtagtc atccgcgaac
     7681 gccatccaag agcggaccga tgctctctcc tcggggttct ctggcatcag cttgggagag
     7741 tcgtagacct catccaggta ctcgttgacg atgttggatt catagagaga agttccgttc
     7801 acgctcagca ctgggacctt accgtaggga ctaacgctca ggaactcttc gctcttgttg
     7861 ctcaggtcaa cctcgtggac ttcgaagtcg atccccttct cgtgcagcac catcctggtg
     7921 cgcatggcgt agggtcattg ctgagtgttg tagagct
//
LOCUS       DASTKM010000079         4838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_369226, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000079 DASTKM010000000
VERSION     DASTKM010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369226"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VFQ10_03115"
     CDS             complement(<1..1091)
                     /locus_tag="VFQ10_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9964362.1"
                     /translation="MVEILLQKNVPAEMRDGATLMSNVYRPAGGGGYPVLLTRLPYGK
                     DLPSVTSVLDPVTAAEAGYIVVVQDVRGRYRSEGKFVPFVKEYEDGYDTVEWASKLPG
                     SNGSVGMYGLSYFGKTQWHAAVMGPPSLKSMVPGLTWGNHLNGVQMRGGVQELGLMQY
                     WAQTSLTLDLLFRKYAGDREIVGEKLPEVIHTIDTLLAGGGYDVLPLTDLPNPDELVP
                     FVEGGFGRGVDHEDWEYLNIEGKYGNVDAPTFHIGGWYDCFIGETLRQYEAMKERSRE
                     AGIRPPHLLVGPWTHGDFGSTFGELDFGIGSSGMFLDYRGDLTDAHLRWFDATIKGDE
                     GALRAIPPVKVFVMGENRWRGYEEWPPPGS"
     gene            complement(1091..1825)
                     /locus_tag="VFQ10_03120"
     CDS             complement(1091..1825)
                     /locus_tag="VFQ10_03120"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9964363.1"
                     /translation="MKVIGAGFGRTGTWSLKAALEKLGFGPCYHMTEVFAHPEHADLW
                     VSAWRKEPVDWDKVLEGYEATMDWPACTFYEELMERNPEAKVLLSVRDPERWYESTRT
                     TIYELSMLLDSSAIARLIFGLSSFLVFGGFAGGRSSLVNDMIWQGTFDGCFEDKAYAI
                     EVYERHNDEVKRRVPSERLLVYEVKEGWGPLCEFLGVPEPEEPFPHLNDAALMRRGTK
                     AVRVLATALPVALSIAAALVLLRRRA"
     gene            complement(1827..3452)
                     /locus_tag="VFQ10_03125"
     CDS             complement(1827..3452)
                     /locus_tag="VFQ10_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET9964364.1"
                     /translation="MLEGLVQDFQLTLPYFLRRAEQLYGYREIVTRRPDKSFHRHTYA
                     DFVSRAKKLAVALGNLGLEPGDRVGTLGWNTYQHLEAYFGVPSSGLVLHTLNPRLHED
                     DLAYIAGHAEDRVMLVDEPLVPVFEKFRERTGVDHAVVVTEGGDVPEGMISYEDLIGD
                     SDEADFEYPDFDENQAAAMCYTSGTTGRPKGALYSHRSTVLHSMMAAMGNTFALSEAD
                     CVLPVVPMFHVNAWGLPFASVLVGAKLMMPGPNLDAASLLEDLEQEEVTLTAGVPTVY
                     LAVLQALDAEPERYDLSKLRAMVIGGSAAPKGVIKDYKERHGIEVLHAWGMTEMSPIG
                     TVANLTTEMKKLPEEEQFEYEAKQGNPVPFVEIRARGDEGFVPWDGETMGELEVRGPW
                     VASSYYEAPEGDDKFTDDGWFKTGDVVTIDRFGFIKITDRSKDLIKSGGEWISSVELE
                     NALMAHPAVSQACVIAIPHEKWDERPLAAIVLREGESATEDELHTHLEVDFARFWLPD
                     AYEFVEAIPMTATGKFQKLKLREQFEGYTSPKS"
     gene            3559..3771
                     /locus_tag="VFQ10_03130"
     CDS             3559..3771
                     /locus_tag="VFQ10_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964365.1"
                     /translation="MKGGQDWSRVEEERFEVVAVTLFGKIVVARYATLEQAEWRARKL
                     GEEAERNPRGYVQYLARPAERPAGNQ"
     gene            3886..4008
                     /locus_tag="VFQ10_03135"
     CDS             3886..4008
                     /locus_tag="VFQ10_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964366.1"
                     /translation="MATEYLQFAGALVFFLGLLIGSYWFVYVPDQGQRKRDDES"
     gene            4047..4511
                     /locus_tag="VFQ10_03140"
     CDS             4047..4511
                     /locus_tag="VFQ10_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9964367.1"
                     /translation="MQVSAKTDYALRAASELALAATEDAGPIKGDWISETQGIPKKFM
                     ENILHDLKRSGIVRTRRGASGGYWLALPAEEISLAAIIRAVEGPLANVRGEWPEAVEY
                     QGAAEHLQEVWIAVRANLRAVLESVTLADIVDGNLPSSITKLTSDPEAWVHH"
BASE COUNT          885 a         1496 c         1536 g          921 t
ORIGIN      
        1 gaccctggag gcggccactc ctcgtacccg cgccagcggt tctctcccat gacgaaaacc
       61 tttaccgggg gtatggctcg gagcgcgccc tcgtctccct ttatggtggc gtcgaaccag
      121 cggaggtgag cgtctgtcag atcgcccctg tagtccagga acatgcccga gctcccgatg
      181 ccgaagtcga gctctccgaa ggtgctgccg aaatccccgt gggtccatgg gccgaccaga
      241 aggtgcgggg gacgtatccc tgcctcgcga gagcgttctt tcatcgcctc gtactggcgg
      301 agcgtctccc cgataaagca gtcataccag cctccgatgt ggaaggttgg cgcgtctacg
      361 ttgccgtact tgccctcgat gttgaggtac tcccagtcct cgtggtccac gccgcgcccg
      421 aagcctccct ccacaaaggg gaccagctcg tccgggttcg gaaggtccgt gagcggcagc
      481 acgtcgtagc cgccacccgc aaggagggtg tctatggtgt ggattacctc agggagcttc
      541 tccccgacga tctcgcggtc gccggcgtac ttgcggaaga ggaggtcgag ggttagcgac
      601 gtctgcgccc agtattgcat caggccgagt tcctggacgc cgccgcgcat ctggacgccg
      661 ttcaagtggt tgccccaggt gaggccaggg accatgctct tcagcgaggg cggacccatg
      721 acggcggcgt gccactgggt ctttccgaag tatgataggc cgtacatgcc gaccgacccg
      781 ttggaaccag gaagcttgga ggcccactcg acggtgtcgt agccgtcctc gtactccttg
      841 acgaagggga cgaactttcc ctccgaacgg taacgtccgc gcacgtcctg cacgacgacg
      901 atgtagccgg cctccgcggc cgttacagga tccaggaccg acgtgacaga tgggagatcc
      961 ttgccgtacg ggaggcgtgt caggaggaca ggatacccac ccccgccagc aggccggtag
     1021 acgttggaca tcagggtcgc gccgtcgcgc atttcggcag gcacgttctt ctggagcagg
     1081 atctctacca tcaggcgcgc ctgcggagca gcacgagggc ggccgcgatc gagagcgcga
     1141 ccggtagggc ggtggcaagc accctcaccg ctttcgttcc gcgccgcatc agggccgcgt
     1201 cgttgaggtg cgggaacggc tcctcgggct cgggcacgcc gaggaactcg cacaacggcc
     1261 cccaaccctc cttgacctcg tagaccagca gacgctcgga cgggacgcgg cgtttgacct
     1321 cgtcgttgtg ccgctcgtag acctcgatgg cgtaggcttt gtcctcgaag cacccgtcga
     1381 aggtgccctg ccagatcatg tcgttcacca ggctgcttct gcccccggcg aacccaccga
     1441 agacgaggaa gctgctcagg ccgaagataa gacgcgcgat ggcggagctg tcgaggagca
     1501 tgctcaactc gtagatggtg gtgcgggtgc tctcgtacca gcgctccgga tcgcgcacgc
     1561 tcaggagcac cttcgcctct ggattccgct ccatgagctc ctcgtagaag gtgcaggccg
     1621 gccagtccat ggtggcctca taaccctcga gaaccttatc ccaatccacc ggctctttac
     1681 gccaggcgga aacccacaag tccgcgtgct cggggtgcgc gaagacctcg gtcatgtggt
     1741 aacaggggcc gaagccgagc ttctcgagcg ccgccttcag cgaccacgtg ccggtccggc
     1801 cgaagccggc tccgataact ttcaccttag ctcttgggag acgtgtagcc ttcgaactgc
     1861 tcgcgcaact tcaacttctg gaacttgccc gtagcggtca tcgggatggc ctccacgaac
     1921 tcgtaggcgt cgggcagcca gaacctggcg aagtccactt ccaggtgggt gtgcaactca
     1981 tcctccgtcg cgctctcgcc ttccctgagg acgatggcgg ccagaggcct ctcgtcccac
     2041 ttctcgtggg ggatggcgat tacacaggcc tgggagacgg cagggtgggc cataagcgcg
     2101 ttctccagct cgaccgagga gatccactcc cccccgctct ttatcaggtc tttactgcgg
     2161 tcggtgatct tgatgaaccc gaaacggtct atggtgacga cgtcccccgt cttgaaccac
     2221 ccgtcgtcgg taaacttgtc gtccccctct ggcgcctcgt agtaagaact cgcgacccaa
     2281 ggaccgcgaa cttctagctc gcccatggtc tctccgtccc acggcacaaa gccctcgtcg
     2341 ccgcgggcgc gtatctctac gaaaggcacg gggttgccct gcttggcctc gtactcgaac
     2401 tgctcctctt cagggagctt cttcatctcg gttgtcaaat tggccacggt cccgatgggg
     2461 ctcatctccg tcatacccca cgcgtgtagg acctcgatcc catgccgctc cttgtagtcc
     2521 tttatgacgc ctttgggcgc cgcagacccc ccgatgacca tcgcccgcag cttcgacaga
     2581 tcgtaccttt caggctccgc gtccagagcc tgcaaaacag cgaggtagac ggttgggaca
     2641 ccagccgtga gcgtcacctc ttcttgctcc aggtcttcaa gaagacttgc ggcgtccaga
     2701 ttcgggccag gcatcatcag cttggccccg acgaggacgg aggcgaaggg caggccccag
     2761 gcgttgacgt ggaacatggg gacgacaggc agcacgcagt cagcctcaga gagcgcgaac
     2821 gtgttgccca tcgccgccat catggagtgg agtaccgtcg agcggtgaga gtagagggcc
     2881 ccctttgggc gtcccgtcgt ccccgaggtg tagcacatgg ccgccgcctg gttctcgtcg
     2941 aagtcggggt actcgaagtc ggcctcgtcg gagtcaccga tcaggtcctc gtaggagatc
     3001 ataccttctg gcacatcgcc accctctgtc acgaccacgg catggtccac gccagtacgc
     3061 tccctgaact tctcgaagac gggcaccagg ggctcgtcga cgagcatgac cctgtcctcg
     3121 gcgtggccgg cgatgtaggc gaggtcgtcc tcgtgcagcc tgggatttag ggtgtgcaac
     3181 acaagtcccg atgaaggtac gccgaagtac gcttcgaggt gctggtaagt gttccagcca
     3241 agcgtcccta ccctgtcccc tggctccagg ccgaggttcc cgagggctac ggcgagcttc
     3301 ttggcgcgag agacgaagtc ggcgtaggtg tgtctgtgga aagacttgtc gggtcggcgg
     3361 gtgacgatct cgcggtatcc gtagagctgc tcagcccggc gcaggaagta gggcagggta
     3421 agttggaagt cctggacaag tccctctagc atctcgtctc ctctattggt cgggtgcagg
     3481 gcccgtcaca gatacggccc ccagcgcctg tactccctcc cccggaaagc gttgctaagg
     3541 gcgatcttag caggcagaat gaaagggggt caagactgga gccgggtgga agaggaacgc
     3601 ttcgaagtgg tggcggtgac gctcttcggg aagatagttg tggcccgata cgcgacgctg
     3661 gagcaggccg agtggcgggc caggaagctg ggcgaagagg cagaacgcaa cccgcgcggc
     3721 tacgtccagt acctggcgcg gccagcggaa aggccggccg ggaatcaata acaggacgtc
     3781 cgcgggggca tcatggccct tgttcctgac cgggcggcga agccggcgct gctatcgtag
     3841 aattgtgtgg cggggtgtct gacgttttcg gaacaaggag gttggatggc gacggagtac
     3901 ctgcagttcg ccggggcgct ggtgttcttc ctgggattgc ttatagggtc ttactggttc
     3961 gtctacgtac cggaccaggg ccagagaaag agggacgatg aaagttgaag ccggagggta
     4021 tggccttcca gggagcgtga ggtagcttgc aggtttctgc caagaccgac tacgcgctaa
     4081 gggcggcctc cgagctggcc cttgctgcga ccgaggacgc gggccctata aaaggggatt
     4141 ggatctcgga gacccagggc atccccaaga agttcatgga gaatatactg cacgacctga
     4201 aacgctcagg gatagtccgt accaggcgtg gcgcctctgg cggctactgg ctcgcgttgc
     4261 cggcggagga gatctcactc gccgccatca tccgcgccgt cgaaggaccc ctcgccaacg
     4321 tcaggggcga gtggcccgag gcggtcgagt atcagggcgc cgccgagcac ctgcaggagg
     4381 tttggatcgc ggtccgcgcc aacctgcgcg ccgtcctcga gtccgtaacc ctcgccgaca
     4441 tcgtcgacgg aaatctcccc tcctcgatca cgaagctcac tagcgacccc gaagcctggg
     4501 tgcatcatta gctggtcagc ccttcggcat ttcagctctg ttgcctgaaa gctttggcct
     4561 gtgaccgaga tttgcagatg caacatctgc aaatcgcttc ttgacttcct ggacgaccgt
     4621 ggttaggctc gcgctattgt tcacttgata agtggagatg atgtgagtag agagcgggcg
     4681 aaagagggga cggaggtcaa gcctgttggc ttctgggagg cattctggtt ctgggtgaaa
     4741 ctcggtttca tcaacttcgg gggtcctgct ggccagatcg ccatcatgca ccgcgaactg
     4801 gtcgaaagca aacgctgggt ctcagagaag cagtacct
//
LOCUS       DASTKM010000080         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1251097, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000080 DASTKM010000000
VERSION     DASTKM010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251097"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFQ10_03145"
     CDS             <1..285
                     /locus_tag="VFQ10_03145"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET9964368.1"
                     /translation="IAEEYRKAIASKNGMVPASVLVDGFVRGTWKTKRSRDKVTLEIK
                     PFEPLAKKDRDALAEEGERLIRFTGAESYEIRFAEPWKIHGTSDQSRIGH"
     gene            complement(406..1587)
                     /locus_tag="VFQ10_03150"
     CDS             complement(406..1587)
                     /locus_tag="VFQ10_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HET9964369.1"
                     /translation="MTAVTEPGPEDRTVSPRMRLELLAEVPAFSHLPDPVLEDMASRL
                     TEERFRPADSVVVEGSTDDRLYMIVEGRAEASTTGPSGIVPLATLGPGELFGELSLLE
                     AGSRRQATVAAVEPLLLLGLRAADLRLALDAHPEGRSAFEKLADDLLVTKLLKQASPF
                     STLDGERLRSLAARLERLEVPSGKTIIRQGEAGEECYVLRSGRVEVLAVGAQGDERIL
                     ATLDPGSLFGEAALLTEGPRNATVRAIEPCTLLALRRTDLLEVLGEDRQTRERMLELV
                     RMRDRPRRVRGIEVHHRTTITGETITTLKDARRDTNYRLSPAGWFLWQRLDGEHTLRD
                     LTSEYLAAYEASASHAVVEAVVGLVAAGFVEGVKLSPEVIAEEPTLWQRVRATIRRIL
                     E"
     gene            complement(1808..>2431)
                     /locus_tag="VFQ10_03155"
     CDS             complement(1808..>2431)
                     /locus_tag="VFQ10_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9964370.1"
                     /translation="TLGMLDFGPEDTPRIWHQVGRDLARLHEGVGEYGPVAGLECEPL
                     PDPGPMPDELASAGYFTAEEARWLSGWLDLLSPFALAPLPRRFRHGDLQTTNVIVRPD
                     SPTYLALIDWGACGWGDAAHDFAGIPLRAVPFMLEGYREVAPFPEDETAEARILWRHL
                     QISLYLLGRPPQPGLSWAERPAAMLLEIMRFFLGSPGKPWTDLYLAP"
BASE COUNT          464 a          789 c          738 g          440 t
ORIGIN      
        1 atcgccgagg agtaccgcaa agcgatcgcc tcgaagaacg gcatggttcc tgcctccgtg
       61 ctcgtcgacg gttttgtccg cgggacgtgg aagaccaagc ggagccgtga caaggttacc
      121 ctcgagatca aacctttcga gcccctggcc aaaaaggatc gcgacgccct ggccgaggag
      181 ggagagcggc tcatccgttt cacaggcgca gagtcctacg agatccggtt cgccgagccc
      241 tggaagatcc acgggacgtc ggatcaaagc cggatcggac actgaccgtc gtcgacagcc
      301 tactccagga cgacgtttag atggatactt ggaccttaat tatcccatcg cccaagctcc
      361 tgtgtagacc tgtgaagcaa aggtccaccc cgccaatgta gctctctact ccaggatacg
      421 ccgtatcgtt gctcgcaccc gttgccacaa ggtgggctcc tctgcgatca cttcgggact
      481 cagcttcacc ccctccacga agcctgccgc cactagtccg accaccgcct ccacgacggc
      541 atgcgatgcg gacgcttcgt acgcagccag gtactccgac gtgagatccc gcaaagtgtg
      601 ctccccatcg aggcgctgcc acaggaacca gccggctggg gagaggcgat agttggtatc
      661 gcgcctggcg tccttgaggg tcgtgatggt ctctccggta atagtggtgc gatggtgcac
      721 ttctatcccg cgtacccggc gcgggcgatc gcgcatccgc acgagctcga gcatacgctc
      781 tcgcgtttgc cgatcctcgc caaggacctc cagcagatcc gtccgacgca gcgcgagcag
      841 tgtgcacggc tcgatggctc tcaccgtagc gttgcgcggt ccttcggtga gcagggccgc
      901 ctccccgaag agggagccgg gatctagcgt ggccagaatg cgttcgtcgc cctgcgctcc
      961 caccgcaagc acctccacac gaccgcttcg gagcacgtag cattcttcgc cggcttcgcc
     1021 ctggcggatg atggtctttc cgctggggac ctcgagacgc tcgagccgtg ccgcgaggct
     1081 ccgcagccgc tcgccgtcca acgtggagaa gggactcgcc tgcttgagca actttgtcac
     1141 caacaggtca tctgccagct tctcgaaggc agagcgcccc tcaggatgtg cgtcgagtgc
     1201 caggcggagg tctgctgctc gcaggcccag caagaggaga ggctcgactg ccgccaccgt
     1261 ggcctgacgc ctgcttccgg cctcgagcag ggatagttcg ccgaacagct ccccaggccc
     1321 tagcgtcgcc agaggaacga tgcctgatgg cccggtcgtc gatgcctcgg cccgaccttc
     1381 cacgatcata tagagccggt cgtctgtaga accctctacc actacagaat cggcaggacg
     1441 gaagcgctcc tccgtcaagc gtgaagccat gtcttccaac accggatcgg gcagatggga
     1501 gaaagcaggt acctctgcaa ggagttccag gcgcatcctg ggtgagacgg tcctatcctc
     1561 cggtcccggt tctgtcacgg cggtcacaga ccctttccga gcttccgacg tatcttactg
     1621 gagatcatta aacaatagcc cgcgctcgga tcaaggcgga gcggcttcgt gccggccatc
     1681 gatgagaagg ttgcactttc cctggagtaa gcaaagcggt gaattgtgat gttaataggc
     1741 ctgaccgaag tcgctgtgat accttgtaac cggctgtcta atccgcgctg ctccgctggg
     1801 tctgggttca cggggctagg tatagatccg tccacggctt gccaggcgaa ccgaggaaga
     1861 agcgcatgat ctccagcagc atcgccgccg ggcgctcggc ccacgagagt cctggttgcg
     1921 gtggccgtcc gagaagatag agggagatct gcaaatgtct ccacagtatt cgggcctccg
     1981 ccgtctcgtc ttccggaaaa ggagccacct cccgatatcc ctcgagcatg aaagggaccg
     2041 ccctgagcgg tatgcctgcg aagtcgtggg cagcatcccc ccagccgcac gctccccagt
     2101 cgatcagggc caggtacgtt ggcgaatcgg gacggactat cacgttggtg gtctgcaagt
     2161 ctccatgacg gaaacgcctt ggtagcgggg cgagcgcgaa gggggacagg agatccaacc
     2221 accccgaaag ccagcgggcc tcctcggcgg tgaagtaccc ggccgatgcc aactcgtccg
     2281 gcatcggccc cgggtcgggg agcggctcgc actcgagacc cgctaccggt ccgtactcgc
     2341 ccactccctc gtgcagtctg gcgaggtcgc ggcccacttg gtgccagatc ctgggggtat
     2401 cttcgggacc gaagtcaagc ataccaagcg t
//
LOCUS       DASTKM010000081         3598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_369480, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000081 DASTKM010000000
VERSION     DASTKM010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369480"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFQ10_03160"
     CDS             complement(<1..381)
                     /locus_tag="VFQ10_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9964371.1"
                     /translation="MDNQESFVRVLGQRDVIALSFGAMIGFGWVVLVGTWLLDAGSLG
                     SIIAFLIGGLLVIFVGLTYSELVSAMPKAGGEHNYAWRALGPTGGFVASWAIALGYVS
                     VVAFEAVALPTAVNYLAPLETGSLW"
     gene            complement(1031..2152)
                     /locus_tag="VFQ10_03165"
     CDS             complement(1031..2152)
                     /locus_tag="VFQ10_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HET9964372.1"
                     /translation="MRITGIEIVPIKLPLLEPFVVSYGTFPDLASVLVQLETNEGLTG
                     WGEGTPDPHVTGETFEGVVATLRHLAPALLGRNPLDRSAAMQLLGSRIAGAPAAKAAL
                     DIALHDLAGRVADLPVWALLGGRAREALSISRVVSLKSPEAMALDARQHVAAGFGTVK
                     LKIGDASDIRGDVRRVAAVREAIGPDIGIKIDVNGGWHTAGAAVGAARGIVPYDPEYL
                     EQPVGRRDLEGMAEVRRLCGVPVMADEAVLDARDALRAVRVRACDLINIKLMKCGGLL
                     AALALNTVAETAGVGCQIGTMVESSVASAAGLHLALALHNAATVEMGGPIMLAEDVGG
                     LRAYYEHDRVTVPDSPGLGVEPDEAVLKRYAGQRDWITA"
     gene            complement(2206..3006)
                     /locus_tag="VFQ10_03170"
     CDS             complement(2206..3006)
                     /locus_tag="VFQ10_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964373.1"
                     /translation="MVGERKLLFMRLRSRLDLEKTERLQRSVFGVSDRDLASSSILVA
                     VHETGGEVLGAFDGELLVGFVSGWGGYVDGRTRLVSDLMAVEVGYRGGIGFALKGLQA
                     VVASQAGFEEITWTVDPLRAANARLNFERLGAHARKYLRNVYGEHFGEGLYGGLPSDR
                     LFVTWPLRSARVRSRLLGRYEPLAPDALATLPEYAPGASESRVRLAVPGDFDGLLASD
                     PVRAREWRFRLRDNLEAAFASGYVITGFGGRRGEPRGYYLLERDLVFE"
     gene            complement(3191..>3598)
                     /locus_tag="VFQ10_03175"
     CDS             complement(3191..>3598)
                     /locus_tag="VFQ10_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HET9964374.1"
                     /translation="VLFPLAIPLTWGVLQANEMANPANYFILYAAIRSILSGAVFGDH
                     CSPISDTTILSSLASGCDHIDHVRTQLPYALLVGVVCVTFGLLPIGFGVPGWILLIVM
                     VAILLAALYLLGKRVDEAEDVSQRRAPTPRPAS"
BASE COUNT          722 a         1188 c         1060 g          628 t
ORIGIN      
        1 ccagagggat ccggtttcca aaggagccaa gtagtttaca gcagtgggga gagccaccgc
       61 ctcgaaggcg accaccgaca catacccgag cgctatcgcc catgaggcga cgaacccacc
      121 cgttggtccc agcgccctcc aagcgtagtt gtgctccccg cctgccttgg gcatcgccga
      181 gactaattcc gagtacgtca gacctacgaa aatgaccaga aggcccccga tcaggaaagc
      241 gatgattgat cctaggctac ccgcgtccag cagccaggtt ccgacgagta ctacccaccc
      301 gaacccgatc atggcaccga acgacagggc gatcacatcc ctttgtccca gaactctgac
      361 gaagctctct tggttatcca cgaacctttc ctcctcgccc ctagctaaca tccctggaaa
      421 cgccccagcg tctccaagct actcatatca tatgtctgcc acttagcagg gtcgagtgac
      481 aacggtgtgc gctctgccag caaccgtgca cagaaaggct ctcgaagacg tcgtcagtag
      541 ggaacgagga tctggagcgc ttggaggccc tcgctagtgg cctcagggcg agcgacccgg
      601 agacgtggca cttatcgtag ttcagccaag cgctcgccgc gcggtcgggt ccgtgagtga
      661 gtcctcgagt gtcttcgacc tcccttcatg ggcagaggtc ccaaggagat cgagggagca
      721 ctctttgcgc gcgttggcat caggttggca tcaagtaggt cgggcaagca accggccctc
      781 cgtcttccag cgttttgcct gcaaatcaag taaaatgtgg aaccgacgag gagactcgaa
      841 cccttgggct tgctccagct atgagtatgc ggtccggggt tgctcagcgt tgcaggggta
      901 tgcaaatcag cgtgctgcag acggggtctg taccttccta gggcttctgt ttcccgctga
      961 cgcgcacgtg gcttcccgcg acaccgcact cgaacgcagg cgggtatcgt caggcggcca
     1021 gcggctggta tcaagcggtg atccaatcac gttggccagc gtagcgcttt aggacggcct
     1081 catccggttc gacaccgagg ccggggctat caggtacggt gacccggtcg tgctcgtagt
     1141 aggcacgcaa gccaccgacg tcctcggcca gcatgatcgg tcctcccatc tctaccgtcg
     1201 cggcgttgtg gagcgccaag gcaaggtgca ggccggccgc cgatgccact gaagattcga
     1261 ccatggtccc tatctggcac cctactcctg cagtctctgc gacggtgttg agcgccagcg
     1321 cggcaagcag ccctccgcac ttcatcagct tgatgttgat cagatcgcaa gcccgcactc
     1381 gaaccgctcg cagcgcgtcg cgggcgtcca gtacggcttc gtcggccatc accggcacgc
     1441 cacacaaccg gcgcacctcg gccatacctt cgaggtcgcg ccgaccgacc ggctgctcta
     1501 ggtactcggg atcgtagggg acaatgcccc tcgcggcgcc aactgccgca ccagccgtgt
     1561 gccagcctcc gttcacatct atcttgatcc cgatgtccgg cccgatcgcc tcccgcacag
     1621 ccgcaactcg ccgcacgtcg cctcgaatgt cgctagcatc tccgattttg agtttgaccg
     1681 tgccgaagcc tgccgcgacg tgctgtctgg cgtcgagagc catcgcctcg ggagacttca
     1741 ggctgacgac ccgggagatg gacaaggcct ccctggcgcg cccgcctagc agcgcccaga
     1801 ctggcaggtc ggcgacgcgg ccagcgaggt cgtgcagcgc gatgtcgagc gcggccttcg
     1861 ccgccggtgc gcctgcgatc cgcgatccca gcagctgcat cgcggcggac cggtctagag
     1921 ggttccgccc cagcaacgcc ggggcgaggt ggcgcagcgt ggcaactacc ccctcgaagg
     1981 tctcaccagt tacgtgtggg tcgggggtcc cctctcccca gcccgtcagc ccctcgtttg
     2041 tctccaactg caccaacacg ctcgctagat ccggaaacgt gccgtaagag acgacaaacg
     2101 gctccagcag tggcagcttt atgggtacga tctcgatgcc ggtgattcgc actttgcctc
     2161 ctcgaatacc ctcgagtgca gagaatcagg tagagctact cagtctcatt cgaagaccaa
     2221 atcgcgttcg agcagatagt aaccgcgagg ctcaccgcgt cgacccccga agcccgtaat
     2281 gacgtagccg gacgcgaagg ccgcctcgag attatccctc agccgaaacc gccactcgcg
     2341 tgccctcacc ggatcagaag cgaggagacc gtcgaagtcg ccggggacgg cgagacggac
     2401 ccggctctcg gatgcaccag gagcatattc aggtagtgtg gcgagcgcgt caggcgcgag
     2461 cggttcgtac cttcccagca gcctcgacct cacacgagcg ctgcgaagcg gccaggtgac
     2521 gaacaggcga tcgcttggca ggcccccgta gaggccttct ccgaaatgct ccccgtacac
     2581 gtttcgcaag tatttgcgcg cgtgggcgcc gaggcgctcg aagttcagcc gcgcgtttgc
     2641 agcccgcagt ggatcaaccg tccacgtgat ctcctcgaaa ccagcctggg aagcgacgac
     2701 ggcttggagc cctttcagag cgaacccgat accgccccgg tacccaacct ctaccgccat
     2761 caagtcggaa acgagacgcg tccggccatc cacgtaacct ccccagcccg acacgaaacc
     2821 taccagcagt tccccatcga aagccccgag tacctccccg ccggtttcgt ggacggcgac
     2881 gagaatgctg gatgaagcta ggtcgcggtc agataccccg aagaccgagc gctgaagtcg
     2941 ttcagttttc tctaggtcga ggcgcgagcg caggcgcatg aaaagcagct tccgctcacc
     3001 gaccatcttc caccaccctg cagagccgct ctcgaaaccc aagatcgtga gagcagtcga
     3061 atctgccaat gacggcacgc tcactgttgc cgctcgaagc cctggtaggc tgaatctagc
     3121 gtccacggga ggactctctg ttgccactgc tccaagtccc gagaagccga acagagcaaa
     3181 ttggggtctc ttaagaggct ggtctcgggg tgggagccct tctctggctc acgtcctcgg
     3241 cttcgtccac cctcttgcct aacaggtaga gggctgcgag aagtatcgcc accatcacga
     3301 tcagcagaat ccaccccggc accccgaacc cgattgggag taggccgaag gtaacgcata
     3361 cgacccccac caggagtgcg tatgggagct gggtgcgaac gtggtcgatg tggtcgcaac
     3421 ctgaggctag cgaagagagg atcgttgtat cagagatcgg ggagcaatgg tcgccgaaga
     3481 ccgcacccga caggatcgag cggatcgccg cgtacaggat gaagtagttc gcggggttcg
     3541 ccatctcgtt ggcctgcagg acgccccacg taagcgggat cgccagcgga aagaggac
//
LOCUS       DASTKM010000082         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_17365, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000082 DASTKM010000000
VERSION     DASTKM010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17365"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFQ10_03180"
     CDS             <1..652
                     /locus_tag="VFQ10_03180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9964375.1"
                     /translation="PQMLMFDTWEDFAKGVGADKYGVDKLTASYTIIQSDHAGTHMDA
                     VKHIRPDGPGASEIPLEYCFSDGVVLDFRHKEYGAGISAAEIDEALEKIGYELKEKDI
                     VLIQTGASAYNDDQERYLRDHCGMTRESTLHLISGGVRMMGIDAVTFDPPVWAMFERE
                     QFWEAHRVMWEEDYWHLENLTNLDQLPPYGFKLSVLPIKWVGTTGAPVRAVAILDD"
     gene            772..1575
                     /locus_tag="VFQ10_03185"
     CDS             772..1575
                     /locus_tag="VFQ10_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9964376.1"
                     /translation="MAGGKGASLARMTAEGLPVPPGFVIPSYVLQESIDAERMLELAA
                     SHSTRELQELVAATEPPREGITAAYENLVGGRLTATYKLVGNKKVAVRSSAVAEDSEA
                     ASYAGQQETYLFVEGGEEVCRRVVDCWASFFSERALFYRSQKGSLEDLRMAVVVQKMV
                     DPEKSGVIFTVDPVRRRRDRMVVEAVRGIGERVVSGEVTPDHYALDRKGKVKREKVVD
                     DRVLTDDELQRLGDLGRQLEERHGAAQDIEWAMIGEEIYLLQSRPVTTM"
     gene            1613..>2338
                     /locus_tag="VFQ10_03190"
     CDS             1613..>2338
                     /locus_tag="VFQ10_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET9964377.1"
                     /translation="MAMIAPEWHEPRSLDEALSLRAEHGEEATVVAGGSFLGIVMNQR
                     LFAPTMLVALRSVPDLDYIGADGGPLRLGAMTTHRAVERSPVVKEGWPSLAFTFSVVA
                     SPRVRNQATVGGVVADADYASDPPSMLAALEARVVARSVRGEREIPMDVFVLDHYETA
                     LEEDELVTELRIPRSEGRSVYRKFCSRSGEDRPCVSVAAAQEDGGLRVVVGAVAGRPQ
                     YFPEICDLARGERLSGEVATEIGR"
BASE COUNT          430 a          726 c          790 g          392 t
ORIGIN      
        1 gcctcagatg ctgatgttcg atacctggga ggacttcgcg aagggggtcg gggcggataa
       61 gtatggcgtg gacaagctga ccgcctccta cacgatcatc cagagcgacc acgccggcac
      121 ccacatggac gccgtaaagc atatccgacc cgacggtccg ggcgcgagcg agattccgct
      181 cgagtactgc ttctccgacg gcgttgtcct ggacttccgc cataaggaat acggggcggg
      241 tatctcagca gccgagatag acgaggcgct cgaaaagatc ggctacgagc tcaaggagaa
      301 ggacatcgtc ctgatccaga ccggagcctc cgcctacaac gacgaccagg aacgctacct
      361 ccgcgatcac tgtggcatga cccgggagtc cacgctccat ctcatctcag gaggcgtccg
      421 catgatgggc atagacgccg tgaccttcga cccgccggtg tgggcgatgt tcgagcgcga
      481 gcagttctgg gaggcccacc gcgtcatgtg ggaggaggac tactggcacc tagagaacct
      541 gactaacctc gaccagcttc ccccgtacgg cttcaagctc tccgtgttgc ccatcaagtg
      601 ggtcggtacc acaggagccc ccgtccgtgc cgtcgccatc ctcgacgatt gagggccggg
      661 gcggagaggc cgttggatat ggacaaacgg gaaagagaaa agtccgcttt gggtaatggg
      721 cagaggagcg ccgtcgtctg gttcacggag gaaacttgcc aggatgtggc ggtggccggc
      781 ggcaaggggg ccagtctggc ccgcatgacc gccgagggac tcccggtgcc cccgggcttc
      841 gtgattccct cgtacgttct tcaggagtcc atcgacgccg agcggatgct ggagctcgcg
      901 gcctcccact ccacgagaga gctccaggaa ctcgtcgcgg ctaccgaacc cccgagggag
      961 ggaataacgg ccgcctacga gaacctcgta ggcggtcggc tcacggctac gtacaagctt
     1021 gtgggcaaca agaaggtggc cgttcgttcc tccgccgtcg cggaggactc cgaggccgcg
     1081 agctacgccg gtcagcagga gacctacctc ttcgtcgaag gtggcgagga agtctgcagg
     1141 cgggtcgtgg actgctgggc gtctttcttc tccgagcgcg ctctcttcta tcgttcgcag
     1201 aagggctccc tcgaagatct gcggatggcc gtcgtggtcc agaagatggt agaccccgaa
     1261 aagtccggcg tgatcttcac cgtggaccct gtcaggcgcc gccgcgaccg gatggttgtc
     1321 gaggccgtca ggggcatcgg ggagcgggtc gtctccggcg aggtgacccc ggaccactac
     1381 gccctggacc gcaagggtaa ggtcaagcgc gagaaggtcg tggatgaccg cgtccttacc
     1441 gacgatgagc tccagagact cggcgacctc ggtaggcaac tagaggaacg tcacggcgcc
     1501 gcacaggaca tcgagtgggc gatgattggc gaggagatct acctcctcca gtcccggccc
     1561 gtcacgacga tgtagacgca ctatttgacg cggttctgga aggatttttg agatggcaat
     1621 gatcgcaccg gagtggcacg agccacgctc gctagacgag gctctctccc tgcgtgccga
     1681 gcacggggag gaggcgaccg tcgtcgcggg cggctccttt ctcgggatcg tcatgaacca
     1741 gaggctcttc gcccccacaa tgctcgtcgc ccttcgcagc gtcccggatc tcgactacat
     1801 aggggccgac ggcggaccgt tacggctcgg ggcgatgacc acccaccggg ccgtagagcg
     1861 ctcgccggta gtgaaggagg gctggccgtc gcttgccttc accttctccg tggtcgccag
     1921 cccgcgtgtc aggaaccagg cgaccgtcgg cggggtcgtc gccgacgcgg actacgcttc
     1981 agatccccct tcgatgctcg cggctcttga ggcgcgggtc gttgcgcgga gcgtgagggg
     2041 agagcgcgag ataccgatgg acgtgttcgt cctcgatcac tacgagacag cactcgaaga
     2101 ggacgagctc gtcacggagc tccggatacc ccgcagcgag ggacgctccg tctaccggaa
     2161 gttctgctcg cgctcaggcg aggataggcc ctgcgtctcc gttgcggcgg cccaggagga
     2221 cggcgggcta cgggtcgtgg tcggcgcggt cgccggacgg ccccagtact tcccggagat
     2281 ctgcgaccta gcgagggggg agcgcctgag cggcgaagtc gccaccgaga tcggacgc
//
LOCUS       DASTKM010000083         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1251486, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000083 DASTKM010000000
VERSION     DASTKM010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251486"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFQ10_03195"
     CDS             <1..338
                     /locus_tag="VFQ10_03195"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET9964378.1"
                     /translation="SVIILATALGSGELILWPYITTQVGIALVWLSVVGISVQFFLNM
                     EVERYTLVTGETAVTGFSRMWIGWSVLFVLGAILPNTFPGWAASGAELFTYIFGLGEG
                     AIPIPLSLT"
     gene            complement(1101..3116)
                     /locus_tag="VFQ10_03200"
     CDS             complement(1101..3116)
                     /locus_tag="VFQ10_03200"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET9964379.1"
                     /translation="MSEPRNLTQKLLGTHLTEGDLVSGEEIDLTVDQILIEDATGSMT
                     ALQFEALGAELVTVPLAVMYVDHNVLQIDDKNMDEHRYLQSFSARYGIRYSRPGNGIS
                     HYVHLERFAKPGELLVGADSHSTMAGAVGMFAVGAGGLDVAVAMAGYGFSLEYPKIVG
                     VELHGELPAWVQSKDIILELLRRYGVRGGVGRVFEFTGDGVSTLSVTDRGTICNMITE
                     LGATAAVFPSDEQTREWFATQRREEDYVPLAADTSATYDEDESIDLSDLEPLIAKPTS
                     PGNVVPVREVAGTETVQVCVGSSVNSSYEDLAAAAAVLRWNVVHPRIEMTVTPGSRQI
                     LDTISKSGVYQDFVAAGARMLEPVCGPCIGVGQAPSAGVPSVRTFNRNFPGRSGTQGD
                     QVYLCSPATAAVTALKGEIADPRELEDPPKITPAPSNPLINDRQILDPPPPEEARSVE
                     IVRGPNIVPPPEGRPLPEVLEGRITFVVEDDVSTGDMAPDGALGMSLWSNIPECAKYM
                     FRRQDPAFHDRVLEWGGGFIVGGHNYGQGSSREQAALAPLHLGIRAIVAKSFARIHRR
                     NLISQGILPLHFVNEADYEQVSQGETWRIESVREVVSVGGAILAAKSDAEREIELEAR
                     LLAREREILVAGGMLKYLRGGSQKRIGIVEGDSASAESGRPESGGRS"
     gene            complement(3184..>3638)
                     /locus_tag="VFQ10_03205"
     CDS             complement(3184..>3638)
                     /locus_tag="VFQ10_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964380.1"
                     /translation="LRDVSARSLGKRGPQVARSIPRNSRQVIQLLPFPGNMRLDRRHR
                     HLGSSARDQGGKDNPEWFFLTGIGCASEHESEGAAGALNSCGDAGVGRLEAEAVPSFD
                     GADLLGEVAGHREKIRRINIGGDVSSEASHSLGGQRRRYEQPLRLEVQ"
BASE COUNT          680 a         1110 c         1109 g          739 t
ORIGIN      
        1 cgagcgtcat tatccttgcc acggcgctcg gctccggcga actgatcctg tggccctata
       61 taaccactca ggtgggaata gctctcgtgt ggctctccgt ggtcggcata agcgtgcaat
      121 tcttcttgaa catggaggtc gaacgctaca cgttggtgac tggcgagacc gccgtcacgg
      181 gctttagccg catgtggata ggctggtccg tgctcttcgt tcttggggcg atcttgccga
      241 acaccttccc aggatgggct gccagcgggg ccgagctgtt cacatacatc ttcggtctcg
      301 gggagggtgc catacccatc cctttgtctc tgacgtagtt gctctgcacc aggttgttgg
      361 ccccacccgc cccagcgaag gctagcgccc ccaggattga gacagccccg acctcttgcc
      421 agtagccagg gaaattcgcg atggcctgag gagcctcggt gacaacgcct gcccaggccg
      481 acagatcggt ggctatgaat atcgctagtg cgatgaagag cagaatgaca atgaccagca
      541 cagcctggac cttctcgagt agctgataga ccactggaga gagggtaacc gacaaggcga
      601 tactgatcag gaatatcgtc gcgacgatgg gaatagcgcc ttcgccgagg ttgaagatgt
      661 atgtgaatag cgatgccccg ctggcggccc atcctgggat ggtgttcggc aagatcgccc
      721 cgaggtcgtc gtgctcgagt tcgcttcctt cagcaaaggt ggtaaatcta ggcttttctg
      781 cctcgatttc ttagtgccag tgcagatagt acagagagcg gacgagcgga ctcgaaccgc
      841 ttacctggtc tctttacgag tgatgattca tgcgttgcaa gggcttgcac ggggttgcag
      901 atcccgcata tctaagctgg tttcctttct gtgccttgtc ctgcgttgca ccgtatagcg
      961 ttcccggtgg tgtcagagtg gtgtcaatat cgctctatgc tgtccgcgta gcatgtcgcg
     1021 gattgagctg tccgcaatca catagaaggg gaagcggcgc tggctcttct cccaagcgcg
     1081 aactcggaaa gagatcatag tcaggaccga ccaccagact caggacgacc tgactccgcc
     1141 gaagcgctgt cgccttcgac aatgccgatt cgtttctgac tacccccacg caggtacttg
     1201 agcatcccgc cggccacgag gatctcgcgc tcacgagcaa gaagccgagc ttcgagctcg
     1261 atctctcttt cggcgtcgct ctttgccgcc agtatcgccc ctccgaccga gaccacctca
     1321 cgaacggact cgattctcca agtctcaccc tggcttacct gctcataatc agcctcgttt
     1381 acgaagtgga gcggcaagat gccctgggag atcaggttgc ggcgatggat ccgcgcgaag
     1441 ctcttggcca cgatcgcgcg gatgccgagg tgcagtgggg caagtgcggc ctgctcgcgg
     1501 ctagatccct gcccgtagtt gtgcccgccc acgatgaagc cgccgcccca ctccaacacc
     1561 cggtcgtgga acgccgggtc ctgccggcgg aacatgtact tcgcgcactc ggggatgttt
     1621 gaccagaggc tcatccctag agcgccgtcg ggagccatat cccccgtgga gacgtcgtcc
     1681 tcgacgacga aggtgattcg tccctcgagc acctcgggga gcggtcgtcc ctcgggcggg
     1741 ggaacgatgt tcggtcctct tacgatctcg acggaccgag cctcctcagg tggaggtggg
     1801 tccaggatct gacgatcgtt tatcagcggg ttggagggcg caggcgtaat tttcggtgga
     1861 tcttcaagtt cgcgcgggtc ggcgatttcg cccttgagcg cggtcaccgc agccgttgcc
     1921 ggagagcaga ggtagacttg atccccctgc gtcccgctcc ggcccgggaa gttgcggttg
     1981 aaggtcctga ccgacggtac gccagcggag ggagcttgcc cgaccccgat gcagggtccg
     2041 cagacgggct cgagcatcct tgcgccggcg gcgacgaagt cctggtagac tccgctcttc
     2101 gagatagtat cgagaatctg gcgcgagcct ggcgtcaccg tcatctctat gcgggggtgt
     2161 actacgttcc accggagaac ggccgcagcg gcagcgagat cctcgtagga ggagttcacc
     2221 gaggagccaa cgcacacctg gaccgtctcc gtaccggcaa cttcacggac cggcacgacg
     2281 ttgccgggag aggtgggttt cgcgatcagc ggctctaggt ccgagagatc tatggactcg
     2341 tcctcgtcgt aagtggcact ggtgtcggcg gcgagcggga cgtagtcctc ctcgcgcctc
     2401 tgggtagcga accattcgcg agtttgctcg tcgcttggga agacggcggc agtggcgccc
     2461 agctccgtga tcatattgca tatcgtgccg cgatccgtca ccgagagggt cgaaacccca
     2521 tctccggtga actcgaagac ccgtcccacc cctccgcgca ccccgtagcg tcgcaggagt
     2581 tcgaggatga tgtctttcga ttgcacccag gcaggtagct ctccgtgcag ctcgacaccc
     2641 acgatcttgg ggtactccag cgagaagccg taccctgcca tggcgacggc aacgtcgagt
     2701 ccgccggcgc cgacggcgaa catcccgacc gcgccagcca tggtggagtg cgagtcggca
     2761 cctaccagga gctcaccggg tttggcgaac cgctcaaggt ggacataatg cgagattccg
     2821 ttgccggggc gggagtagcg gataccgtag cgcgccgaga atgattgtag gtagcgatgc
     2881 tcgtccatgt tcttgtcgtc gatctgcaag acgttgtggt cgacatacat gacagcgagc
     2941 gggacggtaa ccagctccgc cccaagcgcc tcgaactgga gggcggtcat agagccagtg
     3001 gcgtcctcga tcaggatttg atcgacggta aggtctatct cttccccgga aacgagatca
     3061 ccctcagtca ggtgagtgcc cagtagcttc tgcgtgaggt ttcgtggctc actcatgttc
     3121 tcctcctcta taatcgagac cggtgcgtaa attctcggaa cgggcacccg gaccatggat
     3181 ccatcactgg acctccaagc gcaacggctg ttcgtagcga cggcgctgac cacccaggct
     3241 atgagatgcc tccgaggaca cgtcgccgcc aatgttgatc cggcggatct tctcgcgatg
     3301 gcccgccacc tcaccgagca gatcggcccc atcgaacgaa ggtacggcct ccgcttcgag
     3361 ccgtcctacc ccggcgtcac cgcaggagtt gagagcgcca gcggcaccct ccgactcgtg
     3421 ctcgcttgca cagcctatac cagtgaggaa aaaccactcg gggttgtctt taccgccctg
     3481 atctctggcc gacgacccca ggtgtcggtg gcgccggtcg aggcgcatgt tcccagggaa
     3541 tggcaagagt tggatgactt gccttgagtt cctcggtata gagcgtgcca cttgtggtcc
     3601 gcgtttgccc aagcttctag cactcacgtc acgaagac
//
LOCUS       DASTKM010000084         7713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_17950, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000084 DASTKM010000000
VERSION     DASTKM010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7713
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17950"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..844
                     /locus_tag="VFQ10_03210"
     CDS             263..844
                     /locus_tag="VFQ10_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HET9964381.1"
                     /translation="MESLFLAGLGVGLASVAGLRAFVPLALFSLFARLGFVVEPDILS
                     PQIGWTIVLVLSALAVVEIMLDKVPALDPAFGYVMIPIRMVAGAVLFAAVYEPGTLFG
                     GAAPGLVAGGGIAAVVAGLKVILRPPSRSSSAGVSTAFLSAMEDFVAVSGGVVGLFLP
                     FLPLLFVGFLLFFYNRIRKRRGRKYGGLRILGD"
     gene            872..1855
                     /locus_tag="VFQ10_03215"
     CDS             872..1855
                     /locus_tag="VFQ10_03215"
                     /inference="COORDINATES: protein motif:HMM:NF003259.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HET9964382.1"
                     /translation="MDEREITIVKGGRETPFSRGILAQTLAQAGARAELAHQIASEVR
                     AELLAEQRYTVEEEEVLARVRNKLVKKDAFVVGRLDKWRVLRESTEPIVVLIGGATGV
                     GTSTLAADVARRLNIQSVVGTDSIREVLRRAISPDLLPLLHKSSYKIEREDLRVPVEE
                     DEMVLFGFRQQASQVAVGVEAIVDRGLKEGTNLVVEGVHLVPEIILNRYRDHPNVCTL
                     VVYLSDEHVHRSRFYIRALGTAMRRPAEEYISYFEEIRQIHDYIVESSARAGVQAVEN
                     VSIENTSDAAVEIVTNRVSGIAERAGRHASAMLLDGSTPRHGIPVTRAKSG"
     gene            1886..2308
                     /locus_tag="VFQ10_03220"
     CDS             1886..2308
                     /locus_tag="VFQ10_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET9964383.1"
                     /translation="MLDRQRLRTFVLGGAVGALVGILLAPRSGKELRGSLANRAGEAR
                     EKGRETYFDAQEQMRERLAEVREGSRQRPEVVDLGDSSPASGGGEAQRPYLRDVSRDV
                     EPEAVEEIADAERSEQLRRKVRETRERLRGPKDSGPER"
     gene            2305..2919
                     /locus_tag="VFQ10_03225"
     CDS             2305..2919
                     /locus_tag="VFQ10_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964384.1"
                     /translation="MKPTSFVPMVGEISAEDASEVPGLGAVISYWTGDRYSAEEWFRE
                     LNDLWGTAGFAVRRGGEVQGFVVYGPPERLARAARYPLGPIDDEAVLLAYAGGDVRTR
                     RRLLVRMLRDLRQRGVGRVEAIASDRGVSNHVPTAFLLESGWRPVRRATYRMSYYTLA
                     HIDLKSAVEVGELARALVGRVKLPGLKSPVPGALARTTGEQRSR"
     gene            complement(2924..3427)
                     /locus_tag="VFQ10_03230"
     CDS             complement(2924..3427)
                     /locus_tag="VFQ10_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964385.1"
                     /translation="MRDIIFWLSGAALAFLVTAGAFLLLANLASVPSQKRLNPDQARA
                     GSGQSVDLILDREQLASLRYQQDQSLALVVENEGGEHLSDVNVNLKVFSENTALSDSR
                     YYRQTVQKIPAGEAASVRFEFNLLVREEPVARPPTANPEPSRKILEIRATTPGGTSTV
                     RTVILPP"
     gene            complement(3424..4632)
                     /locus_tag="VFQ10_03235"
     CDS             complement(3424..4632)
                     /locus_tag="VFQ10_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964386.1"
                     /translation="MLSRVGSAKRVAVLSVAAVIVAGCAHAHKEVSIAPLVPAAGRVD
                     PGLGPGTRALSSGPGYKGSPSWNPGGDRIAFTVDGYVVDRPTDSGNLRYWTTRDFIAE
                     DTEWISEDTLMILGSASPSGTEDTPRSLYRARAEENSLELERVEKEVSAIGPGRGGLI
                     FAIGHRSFESGLALTRGSGEVRRVYSEPIKGRVASLSLSPDGDEAVLAVRPPGDLETS
                     ALHVFDLRKGKGREIMRLEGNQEILGTPQWTGQYLYFVGGETNAPVDSDGSEPLYEIY
                     HVPEEGGAPEPAPGVGEDFVAASIRVSPDGKRLAVIGRLNPTSPTNLYVLDPLAKNLT
                     LVTTNEDMEIKTGPDDLAWSPGGKSVAVIARGTPSTEPEVRADPADRLLRDFYNLYEI
                     PVDGPEDAPP"
     gene            complement(4622..6496)
                     /locus_tag="VFQ10_03240"
     CDS             complement(4622..6496)
                     /locus_tag="VFQ10_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9964387.1"
                     /translation="MGIEEREDNRLPPALSFKARIFVALVGISSVTSLIIGLVLYYFA
                     QDRLVHAENTLLKQRSQTANAGAQDFLEGLRDPEDKTLPPPPDYAEELVRAVADPTGL
                     EVLYVGPDMEPLAARDGLGNPLDPKKAYGRLDRAELEETASSPGGEGRLVWSEGRSGY
                     VAVWPLRTYDGTVKGVMVYHVPRDELGDTLAYLRYGILGAILTSILLAGAASLLLTRQ
                     VTRPLSETKDAAIRVASGDYAATVPVKSRDELGEVARTFNYMAEEIEHYVGEIQEQKS
                     RLEAVLESSPEAVVATDSGERVTMVNAAAARMLGIRASDRGRTLEEIGAPRAVLRCLR
                     EAAANGVAVREVELGEKAFWAYAAQMDRGQPDLAPNGDAGIILAVRDITEHRSLEKAK
                     TDFVSDLSHELRTPLTTIQSAVSLLERARDRLDPLEHRALELADQELQRIRGMVEELM
                     TLAQMDSWKYQLEVGPANMSTVVQTAIESVETKAQRFGIKIYFGDAGEHRCICDVQKL
                     YQVFINLLDNAIKYSDPGARVDVEIEEDDSALTVRIRDTGVGIPKEDLNQLFERFYRV
                     DKDRSRATGGSGLGLAISRQIVEMHGGSISVESEVDVGSVFEVRLPKAFLSRSVSHAL
                     "
     gene            complement(6512..7201)
                     /locus_tag="VFQ10_03245"
     CDS             complement(6512..7201)
                     /locus_tag="VFQ10_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9964388.1"
                     /translation="MDETILLVDDDAALLEVTSIVLASEGYRVVTAEDGAEALRILGR
                     EGLDLVVLDIMLPRMSGFEVLKKLREHSDVPVVLLTAKSQSVDKVVGLELGADDYITK
                     PFDTKELLARIKAILRRFGRDGRGREGLLRLGPLELDPNGYTVSRSGETLDLTPTEFK
                     ILALLMRRPGQAFTRAQISEAVSTGSHYLASRYIDVHISRLRAKVERDPSSPEIVQTV
                     PSVGYRAARPV"
     gene            7382..>7713
                     /locus_tag="VFQ10_03250"
     CDS             7382..>7713
                     /locus_tag="VFQ10_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9964389.1"
                     /translation="MAKSIGIDLGTTNSCVAVLEGGDPKVITNSEGERTTPSVVAFDR
                     RSGERLVGQLARRQAVTNPERTVYSIKRFMGRRYSDVKTEAERVGYQVKSGPGGDIRI
                     EVDDKEYSP"
BASE COUNT         1343 a         2371 c         2472 g         1527 t
ORIGIN      
        1 gaggaacgga ttccaccgta gatctccgag ctctggtaca cgaagcccct tcgcttgcag
       61 aaggccacta tcttgtccat cgtcacggtc tcgggcacgg gtcctccgaa ttcttagggt
      121 caggcaagta tagcccagag agacccccgg attgagcctg gcacatggcg ggagagggac
      181 cgcgtttctc cttcgtcgat gatggaccgc ttcgcgagat ggcctgggcg gagctaccct
      241 gggccgcgct ataatgttgg ccgtggagag tcttttcctg gcaggattgg gtgttggtct
      301 cgcctccgtc gcggggttga gggcctttgt gccgcttgcg ctgttttctc tgttcgcccg
      361 gctcgggttt gtcgtggagc ctgacatcct cagcccgcag atcggatgga ccattgtcct
      421 ggtgctctcc gctctggcgg tcgtggagat catgctcgac aaggtcccgg ctcttgaccc
      481 tgcgttcggc tacgtcatga tacccatcag gatggtggca ggggctgtcc tgtttgcggc
      541 ggtgtacgag cctgggacgc tcttcggcgg cgcggcgccg gggctcgtgg ccggtggagg
      601 gatcgccgcc gttgtcgccg ggctcaaggt tatcctgcgc cctccgtcga ggagttcgtc
      661 ggcaggggtc tcgacggctt tcttgagcgc tatggaggac tttgttgcgg tttcaggcgg
      721 agtagtaggg cttttcctgc ccttcctgcc gcttttgttt gtcgggtttc tgctattctt
      781 ctacaaccgc atcaggaagc ggcgtgggag gaagtacggg ggcctccgca tccttggcga
      841 ttagtattcg tcggcgttct aggagggtgc aatggacgag cgggagatca cgattgtcaa
      901 ggggggtagg gagacaccgt tctcccgagg aattctggcg cagacgctgg cccaggcggg
      961 ggcgcgcgcc gagctggccc accagatagc gagcgaggtg cgggccgagc tcctggcgga
     1021 gcagcgctac acggtcgagg aggaggaggt cctcgcccgc gtcaggaaca agctggtcaa
     1081 gaaggacgcc ttcgtcgtcg gcaggctcga caagtggcgc gtattgcggg agtcgaccga
     1141 gccaatagtg gtcttgatcg gcggggcgac gggggtaggg acaagcacgc tcgccgccga
     1201 cgtggccagg cgcctcaaca tccagagcgt ggtgggtact gattcgatca gggaggtcct
     1261 gcggcgtgct ataagccccg acctgctccc tttactccac aagagttctt acaagataga
     1321 gcgcgaggac ctgagggtac ctgtcgagga agacgagatg gtcctcttcg ggttcaggca
     1381 gcaggcctcc caggtcgccg tcggcgtcga ggccatcgtt gacaggggcc tgaaagaggg
     1441 gacgaacctc gtggtcgaag gggttcacct cgtgcccgag atcatcctca accgctaccg
     1501 cgaccatccg aacgtgtgca cgctcgtggt ctacctctcc gacgagcacg tgcaccgttc
     1561 gcgtttttac atcagggccc tgggcacggc gatgcgtcgt cctgcagagg agtacatctc
     1621 ctacttcgag gagatacgtc agatccacga ctacatcgtc gagagttccg ccagggccgg
     1681 tgtgcaggct gtggagaacg tctccataga gaacacttcg gacgcggcag tcgagatcgt
     1741 gaccaacagg gtctcgggga tagccgagag ggccggccgg catgcctcgg cgatgctcct
     1801 cgatgggtct acacccaggc atgggatccc cgtcacccgc gcaaaaagcg gttagaataa
     1861 cgctggaggc cagaaggaga caaaggtgct ggacaggcag cgactgagaa ccttcgtact
     1921 cggcggcgcg gtaggggccc tggtgggcat actcctggcc ccgcgcagcg gcaaagagct
     1981 caggggttca ctcgccaaca gggctggtga ggcccgcgag aagggccgcg agacctattt
     2041 cgatgcgcag gagcagatga gggagcgtct ggccgaggtc cgcgaaggat cacgccaacg
     2101 ccccgaggtg gtcgacctcg gcgactcctc ccctgcctca ggggggggag aggctcagcg
     2161 accctacctc agggacgtgt cgcgcgacgt cgagcccgag gctgttgagg agattgccga
     2221 cgccgagagg tccgagcaac tacgccggaa ggtgagagag accagggaac ggctgcgggg
     2281 accgaaggac agcggtccgg agcgatgaaa cccacgtctt tcgtgccgat ggtcggtgag
     2341 atctcagccg aggatgccag cgaggtgccc ggtcttgggg ccgtgatctc gtactggacc
     2401 ggcgataggt actccgccga agagtggttt cgcgagctga atgacctctg ggggacggcg
     2461 ggtttcgcgg tgcggcgcgg tggagaggtt cagggattcg tggtctacgg gccgcccgaa
     2521 cgtctggcgc gggcagcgcg ttatccgctg gggccgatcg atgatgaggc cgtgctgttg
     2581 gcctacgccg gtggagatgt acgtacgcgc cgccggctgt tggtgaggat gctcagggat
     2641 ctcaggcagc ggggcgtcgg gagggtcgag gcgatagcca gcgaccgagg ggtgtccaac
     2701 cacgtgccta ctgcattcct tctggagagc ggctggcggc ccgtgcgccg cgccacttat
     2761 cgtatgagct actacacgct cgcgcacatc gacctgaaga gcgccgtcga ggtgggggag
     2821 ctggcacggg cgctggtcgg gcgggtaaaa ctccccgggc tcaagtcacc tgtgcccggt
     2881 gcactggccc gcactacggg cgagcaaagg agccggtagg gcctcagggt ggcaggatca
     2941 ccgtcctgac cgtcgaggtg ccccctggcg tcgtggccct tatctccagg atcttgcgcg
     3001 atggttcagg gttcgctgtg ggtggcctcg caacgggctc ttcgcggacc agtaggttga
     3061 actcgaaacg cacgctggca gcctcgccgg ccggtatctt ctggaccgtc tgtctgtagt
     3121 aacgggagtc cgatagggct gtgttctcgg agaagacctt caggttcacg tttacatccg
     3181 agaggtgctc tccgccctcg ttctctacta ccagggcgag actctgatcc tgttggtacc
     3241 tgagagacgc gagctgttct ctatccagta tcagatccac ggattgtccg gacccggccc
     3301 ttgcctggtc cggattcaga cgcttctggg aagggacgga agcgaggttc gccaggagca
     3361 ggaaagctcc cgctgtgacc aggaaggcga gagccgcgcc ggagagccag aatattatgt
     3421 ccctcatggc ggcgcatcct cggggccatc gaccggtatc tcgtagaggt tgtagaagtc
     3481 tctcaggagc ctgtctgcgg ggtccgcacg cacctcgggt tccgtggatg gggtgccgcg
     3541 ggcgattacc gcgacgctct tcccgccggg cgaccacgcg aggtcgtccg gtccggtctt
     3601 tatctccatg tcctcgttgg tcgttacaag tgtgaggttc ttcgcgaggg ggtccagtac
     3661 gtacaggttg gtcggtgacg tcgggttgag gcgtccgatg acggccaacc gcttgccgtc
     3721 aggagagacg cgtatgctcg ctgccacaaa gtcctctccc acacctggcg cgggttccgg
     3781 cgcgccgccc tcctcgggca catgatagat ctcgtagagt ggctctgagc cgtcgctgtc
     3841 tacaggtgca tttgtctccc cgcctacgaa gtagaggtac tgccccgtcc actggggcgt
     3901 gccgagtatc tcctggttgc cttccagccg cattatctct cgccctttcc ccttccgaag
     3961 atcgaacacg tgcaaagccg aggtctcgag gtctccggga ggacgcacgg ccaggacggc
     4021 ctcgtcgccg tcaggcgaga gggagaggga tgcgacgcgg cctttgatag gctcggagta
     4081 gactctgcgg acctccccac tgcctcgtgt cagcgcgagc ccgctctcga aagacctgtg
     4141 accgatggcg aagatcaaac cccccctgcc cgggccgatc gccgaaacct ccttctcgac
     4201 cctctctagt tccagcgaat tctcctctgc tcgcgccctg tagagggacc tcggggtgtc
     4261 ctctgtccct gagggcgacg ccgagccgag tatcatcagg gtatcttccg agatccactc
     4321 tgtatcttct gcgatgaaat ccctggtggt ccagtacctc aggtttcccg agtccgtcgg
     4381 cctgtccaca acgtagccgt ctaccgtaaa cgcgatcctg tcgccccccg gattccagct
     4441 cggtgagcct ttgtacccgg gtcctgaact gagggctcgt gtgccagggc caagtccggg
     4501 gtccaccctg cccgctgccg ggacgagggg cgcgatggag acctccttat gggcgtgggc
     4561 gcaccccgcc acaatgacag ccgctacgga gaggacggcg acccgttttg ccgaccctac
     4621 cctagagagc atgactcacg gagcgggaga ggaatgcctt cgggagcctg acctcgaaga
     4681 cggagcccac gtcgacctcg ctctcgacgg agatgctgcc gccgtgcatc tccactatct
     4741 gcctacttat ggcgagccca agcccgcttc ccccggtcgc gcgagagcgg tctttgtcca
     4801 ccctgtagaa acgctcgaag agctggttca gatcctcttt ggggatgccc acgccagtat
     4861 cacggatgcg gacggtcagc gccgagtcgt cctcctctat ctctacatcc accctggcgc
     4921 ctgggtcgga gtatttgatc gcgttgtcca gaaggttgat gaagacctgg tagagcttct
     4981 ggacgtcgca gatgcacctg tgctcgcccg cgtcaccgaa gtatatcttg atgccgaacc
     5041 tctgggcctt ggtctctacc gactctattg ccgtctggac gaccgtactc atgtttgccg
     5101 gccctacctc gagttggtac ttccaggagt ccatctgggc cagggtcatc agttcctcga
     5161 ccatccccct tatgcgctgc aattcctgat cggccagctc gagcgcccgg tgctccagcg
     5221 ggtcgagccg gtcccgtgcc cgctccagca ggctgaccgc gctctggatg gttgtcagcg
     5281 gggtcctgag ctcgtgggaa agatccgaga cgaagtcggt cttggctttc tccagcgagc
     5341 ggtgctcggt tatgtctctt acggccagga tgatgccggc gtcgccgttt ggtgcgaggt
     5401 cgggttgccc cctgtccatc tgggcggcgt aagcccagaa agccttctct ccaagctcga
     5461 cctcccttac cgcaaccccg ttggcggccg cttcgcgcag acacctgagg actgcgcgtg
     5521 gggccccgat ttcctccagc gtacgccccc tgtctgaggc ccttatgccc agcatcctgg
     5581 ccgccgccgc gttgaccatc gtgacccgct cgccagagtc cgtcgccacg acagcctcgg
     5641 gagaggattc gaggacggct tccagacggc tcttttgctc ctggatctcc ccgacgtagt
     5701 gctctatctc ctcggccatg tagttgaaag tccttgctac ctcaccgagc tcgtccctgc
     5761 tcttgacggg cacggtcgcc gcgtaatccc ctgaggcgac ccttatggct gcatccttgg
     5821 tctcggagag gggacgcgtg acctgacgcg tgagaagcag gctcgccgcc ccggcgagga
     5881 ggatgctagt cagaatggcc ccaaggatgc cgtagcgcag gtaggccagc gtatctccga
     5941 gctcgtcgcg gggaacgtgg tagaccatga ctcccttcac ggtaccgtcg taggtcctga
     6001 gcggccacac cgctacgtaa cccgagcgtc cctcgctcca cacgagtctg ccctctcccc
     6061 ctggcgatga tgcagtctcc tcgagttccg ctctgtcgag tctcccgtat gcctttttgg
     6121 ggtcgagagg attccccagg ccgtcgcggg cggccagagg ttccatgtcg ggtcctacgt
     6181 agaggacctc caggcccgtc ggatcggcga ccgcccggac gagctcctcg gcgtaatcgg
     6241 gcgggggtgg gagggtctta tcttcggggt cgcgcagtcc ttccaggaaa tcctgcgcgc
     6301 cagcgttcgc cgtctgagag cgttgcttga gcagcgtgtt ctccgcgtgt accagcctgt
     6361 cctgggcgaa gtagtacagg accagcccga taatcaggct cgtcacagaa gagatcccga
     6421 cgagggctac gaagatcctg gccttgaagc tcaacgccgg cgggagccta ttatcttctc
     6481 tctcttctat gcccatcctc cgtcgtggga tctagacggg cctcgcggcc ctgtagccga
     6541 cgctagggac ggtctgtacg atctccggac tcgaagggtc ccgctcgacc tttgccctga
     6601 gcctgcttat gtggacgtct atgtagcggc ttgccaggta atgggacccc gtggagaccg
     6661 cctcggagat ctgggcccgc gtaaaggcct gcccaggacg gcgcatgagg agcgcgagta
     6721 tcttgaactc ggtaggcgtg agatccaacg tctcccccga gcggctgacc gtgtagccgt
     6781 tgggatccaa ctccaaaggg ccgagcctca ataatccctc cctgcccctg ccatcccgac
     6841 cgaaacgcct gagtatggct ttgatcctgg ccagcaactc tttggtgtcg aagggcttgg
     6901 tgatgtaatc gtcggcccca agctccagcc cgaccacctt gtctacagac tggctcttcg
     6961 ccgtgagcaa gaccacaggg acgtcgctgt gctcgcgcaa cttcttcaag acctcgaacc
     7021 cgctcatcct cggcaacata atgtcgagca ccacgagatc caaaccctcc cgcccgagaa
     7081 tccggagcgc ctcggccccg tcctcagccg tcactacccg ataaccctca gatgccagta
     7141 cgatggacgt gacctccaag agcgccgcgt catcatcgac caacaagatc gtttcgtcca
     7201 atccgactcc ctacaccccg agaacataca accagcgtcc accgcaccgg agaccatatt
     7261 atcctaccct ataccaggcc tcctccgtaa cagcaacctg cgacgaaaaa cttgcaacgg
     7321 tgacactcag attttatact gatgacactt tagataacgt tatctcgtgg gaggtatgcg
     7381 catggccaag agcattggca tagatttggg cacgaccaac agttgtgttg cggtactcga
     7441 gggtggggat ccgaaggtga tcaccaactc ggaaggggag aggacgacgc cttcggtcgt
     7501 ggccttcgac aggaggagtg gagagcggct ggtagggcag ttggcgagga ggcaggctgt
     7561 aacgaacccg gagcgtaccg tctactcgat caagcgcttt atggggcgcc gctacagtga
     7621 cgtgaagact gaggccgagc gcgttggtta ccaggtcaag tctggccctg gcggggacat
     7681 caggatagag gtcgacgaca aggagtactc tcc
//
LOCUS       DASTKM010000085         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_18369, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000085 DASTKM010000000
VERSION     DASTKM010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18369"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFQ10_03255"
     CDS             <1..963
                     /locus_tag="VFQ10_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HET9964390.1"
                     /translation="PDLTDSVKEVIGALGVDIEWEIAEAGETVMDREGTPLPESVLDS
                     IRRNKVALKGPLTTPVGTGFRSVNVALRKELDLYGNIRPSLSLPGVETPYENIDIIIY
                     RENTEDLYAGVEHTVGKHAAESIKIITREGTERICRMAFERSKEQGRRHITVVHKANI
                     MKFTDGLFRDVFFEVARDYENDFEEIDDRIVDNMCMQLVMKPNLYDVLVCPNLYGDIL
                     SDLCAGLTGGLGVAPGANIGEEIAVFEPVHGSAPKYAGQNRANPLATLLSAKNMLIHL
                     DYGEEADRMQSAIEAALKNPQTRTKDLGGSAGTREFTRAIVSNL"
     gene            1100..2221
                     /locus_tag="VFQ10_03260"
     CDS             1100..2221
                     /locus_tag="VFQ10_03260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9964391.1"
                     /translation="MRIAFFTETFLPATDGVVTRLRHTIEELLRMGDEMLVIAPKYGE
                     GPGSYEGVPIHRVSSVPFPPYPQIKLAPINPGVGSALRDFGPELIHAVNPFVLGRGAP
                     FYSRRLDVPLVASYHTNIAAYARFYGLGLFDRATRWYTRKLHNRAAINLCTSEATLRY
                     LAHEGVERLHLWPQGVDARRFHPDKFSKDWRVRLTNGHPAERLLLYVGRLGHEKNIAN
                     LRGVLGEVPGVRLALVGDGPARRDLEQEFANTRTVFTGVLQGEELAAAFASADAFLFP
                     STTETLGIAMIEALASGLPVIAARTGATREVVDEGETGLLYDPGSDADLAAAVRKIAS
                     DDGLRARMGRNARASAQRRDWGTSTRTLRGYYEQALGQR"
     gene            2218..2682
                     /locus_tag="VFQ10_03265"
     CDS             2218..2682
                     /locus_tag="VFQ10_03265"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET9964392.1"
                     /translation="MSEAKAKLKSGGKRFSKFSIVGLSNAVIDIGVLNLFLWLEPTRD
                     VSLLVIYNGVALVLANLNSYAWNTLWTFRGRAEHDLRQILLFGLQALVNIVVSNGLFW
                     ALIHPIIVYTEVPTYLAGNVAKIISVTVASTISFFIMRYVVFSRKRWFNERL"
     gene            2716..2788
                     /locus_tag="VFQ10_03270"
     tRNA            2716..2788
                     /locus_tag="VFQ10_03270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2749..2751,aa:Gly,seq:gcc)
BASE COUNT          573 a          856 c          902 g          523 t
ORIGIN      
        1 ccggatctca cggactccgt aaaggaggtt ataggcgcgc tcggcgtcga catcgagtgg
       61 gagatcgccg aagccggcga gacggtgatg gatagggaag gtacgcctct tcccgagtca
      121 gtgctcgact ctatccggcg caacaaggtg gcgctgaagg ggccgctaac cactcccgtc
      181 gggacggggt tcaggtccgt gaacgtagcg cttcgcaagg agctcgacct ctacgggaac
      241 atcaggccat ccttgagcct accaggggtg gagacgccgt acgagaacat cgacatcatc
      301 atctaccgcg agaacactga ggacctctac gccggcgtgg agcataccgt cggcaagcac
      361 gcggcggagt cgatcaagat catcacccgc gagggcaccg agcgcatctg ccggatggcc
      421 ttcgagcggt cgaaagaaca gggccgcagg cacatcaccg tcgtccacaa ggctaacatc
      481 atgaagttca cggacggtct gttccgtgac gtcttcttcg aggttgccag agattacgag
      541 aacgacttcg aggagataga cgatcgtata gtcgacaaca tgtgcatgca gctcgtcatg
      601 aagccgaacc tctacgacgt tctggtctgc cccaacctct acggcgacat cctatcggac
      661 ctctgcgcag gacttacggg tgggctcggc gtcgccccag gggccaacat aggggaggag
      721 atcgcggtct tcgagcctgt ccacggctcg gcacccaagt acgccggaca gaacagggcc
      781 aacccgctcg cgacgttgct ttcggccaag aacatgctca tccatctcga ctatggcgag
      841 gaggccgaca ggatgcaatc ggcgatagag gcggccctga agaaccccca gacgcgtaca
      901 aaggatctcg gcggcagcgc cggaaccagg gagttcacgc gggccatcgt ctcgaacctg
      961 tagaatcggg gtgcggatcg ttacgagcgg acatcggagg caggtgctgg ccggtagaga
     1021 cagaacctgt aggggagcgc tcaggcggcg atacggtgtg gtattagccc ggttcggccg
     1081 cggctagcgg ggaggcccag tgcgcatcgc gttctttacc gagaccttcc ttccggccac
     1141 cgatggcgtg gtcacgagac tcaggcacac catagaagag ctgctccgga tgggcgacga
     1201 gatgctcgtg atcgccccca agtacggaga aggaccgggt tcttacgagg gggttccgat
     1261 ccaccgtgtc tccagcgtgc ctttcccgcc ctatccccag ataaagctcg ctcccataaa
     1321 ccctggcgta ggcagcgcgc tgcgcgactt cgggcccgag ctgatccatg ccgtcaaccc
     1381 tttcgttctg ggacggggag ctcctttcta ctccaggcga ctggacgtcc ctctggtggc
     1441 ttcctaccac acgaacatcg ctgcctacgc ccgcttctac gggctgggac tcttcgaccg
     1501 cgccacgcgc tggtacacca ggaagctgca caacagggcc gccatcaacc tctgcacttc
     1561 ggaggcgacg ctcaggtacc ttgcccacga gggtgtcgag cggctgcacc tgtggccaca
     1621 gggtgttgat gccaggcgct ttcaccccga caagttctcg aaggattggc gggtcagact
     1681 gaccaacgga cacccggcgg agcggcttct gctctacgtc ggacgcctcg gtcacgagaa
     1741 gaatatcgct aatctcaggg gggttctcgg cgaagtacca ggagtgcgtc tcgccctcgt
     1801 cggagacgga ccagcgcgca gggatctcga acaggagttc gcgaatacgc ggacggtctt
     1861 tacgggagtg ctgcaggggg aggagctcgc ggccgcgttc gcctccgccg acgctttcct
     1921 gttcccttcg accacggaga cgttggggat agcgatgatc gaagccctcg cctccgggct
     1981 gcccgtgatc gccgccagga cgggggccac cagggaggtg gtggacgaag gcgagacagg
     2041 gcttctctac gatcctggtt ctgatgcgga cctcgccgcc gccgtccgaa agattgcctc
     2101 agacgacggt ctgagggcac ggatgggacg caatgcccgc gcgtccgccc agaggcgcga
     2161 ttggggaacc tctacgcgca cgttgcgcgg atactacgaa caggcgctgg ggcagagatg
     2221 agcgaagcga aggccaagct caagagcggc gggaagcgct tctccaagtt ctccatcgtc
     2281 gggctctcca acgccgtcat agacatcggg gtcttgaacc tcttcttatg gctcgagccg
     2341 acgcgcgacg tttccctgct cgtcatctac aacggtgtgg cgctggttct tgcgaaccta
     2401 aacagttacg cgtggaatac attgtggacc tttaggggaa gggccgaaca cgacctgagg
     2461 cagatactac tgttcgggct gcaagcgctc gtaaacatag tggtaagcaa cgggttgttc
     2521 tgggccctga tccaccccat catagtgtat acggaggtgc caacttacct ggccggtaac
     2581 gtcgcaaaga tcatctccgt taccgtggct tctaccatca gctttttcat catgcgctac
     2641 gtggttttct cgcgaaagag gtggttcaac gagcgcttgt aagagcccta ggcccgtgct
     2701 aaattagagc cgcacgcgga agtagctcag ttggtagagc atcaccttgc catggtgagg
     2761 gtcgcgggtt cgaatcccgt cttccgctct ggccgtgggg cgtagcggcg ccgctggcgg
     2821 catggccgag tggttaggca agggtctgca aaac
//
LOCUS       DASTKM010000086         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_547022, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000086 DASTKM010000000
VERSION     DASTKM010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547022"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFQ10_03275"
     CDS             <1..184
                     /locus_tag="VFQ10_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964393.1"
                     /translation="LLEQLTRLGVHGRALDPRPSDIYPEHVHEPSRDGYVLILYDPTL
                     PPEVPRWPTHPLAASP"
     gene            complement(299..1141)
                     /locus_tag="VFQ10_03280"
     CDS             complement(299..1141)
                     /locus_tag="VFQ10_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HET9964394.1"
                     /translation="MFSSFVTLLREGFEVTLLVAIVLAYLVKIERREDIRQVWYGVGA
                     ALLVSLAVGGILFVTAGGLEGRAEYIFEGTAMWVAVGFLTYMILWMRRESRNVAQSIR
                     RGIDSAVAKGGSLALVSLVFVMALREGIETALFLFGITRTSEPLQVAFGAALGIAGAV
                     VLGYAVYAGGKRINLGMFFKVTGVFLILVAAGLLAHGVAEFQEAGLIPTIMPLWDVSS
                     SPVLGEGTIASEFLTAFFGWDPEANLLELLAWFTYVVVVGYAFLRPQPLPEGATASPA
                     TRNN"
     gene            complement(1307..>2222)
                     /locus_tag="VFQ10_03285"
     CDS             complement(1307..>2222)
                     /locus_tag="VFQ10_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET9964395.1"
                     /translation="YLEHGRMMPEDGLDTLAAFDEIYLGAVGWPTVPDHLSLWGLLLP
                     IRRTFDQYVNLRPARLLRGVKSPLAEPNDLDITVVRENTEGEYSDSGGRVYRGTPHEI
                     AVQESIFTRRGVERIIRYAFEEAKARRGLLVGATKSNGISVTMPFFDEIFREISSDYP
                     DVEASLMHADALAARLVLAPHSFDVVVGSNLFGDLLSEVTAAIAGAIGIAPSANLNPE
                     GTYPSLFEPIHGSAPDIAGQGAANPSGAIWAGAMMLDHAGYPDASARILSALEETLAS
                     GTKTSDLGGTATTDEMADAVVQRLRIHA"
BASE COUNT          450 a          706 c          668 g          398 t
ORIGIN      
        1 tctcctcgag cagctcaccc gactgggtgt ccacgggcgc gcccttgatc cccgaccctc
       61 cgatatctat cccgaacacg ttcatgagcc ctccagagac ggatacgtcc tcattctata
      121 cgatcccaca ctcccgcccg aggtgccgcg atggccgaca catccattgg cggctagccc
      181 ctaggatcgt aagatggccg tctacgtccc tcggaggcta gcgcttcagt atcgaagcgc
      241 tagcctcgta cgtgacccgt gcggacagta ggagtcttgt aggttccgag ctggaaagct
      301 agttgttacg cgtagctggg ctggcggtgg caccctcggg gagcggctgc ggccgcagga
      361 aagcgtatcc gaccaccacg acgtacgtga accaggcgag caactccagc aggttcgcct
      421 cagggtccca gccgaagaat gcggtgagga actcgcttgc gatggtcccc tcgcccagca
      481 cgggtgagct gctgacgtcc cacagaggca tgatggtggg aattagaccg gcctcctgga
      541 actcggccac accgtgggcc aggagccccg cggcgaccag gatcaggaag accccggtca
      601 ctttgaagaa catgccaagg tttatgcgct tccctcctgc gtagacggcg taacccaaca
      661 ccacggcccc cgctataccg agcgccgcgc cgaaagcaac ctgcaagggc tccgaagtac
      721 gcgtgatccc gaaaaggaag agcgcggtct ctatcccctc acgcaaggcc atcacgaaga
      781 ccagcgagac cagcgcgaga ctgccaccct tcgccacggc gctgtcgata ccccggcgaa
      841 tactttgcgc tacattgcgc gactcccgcc gcatccagag gatcatataa gtcaggaacc
      901 cgacggcaac ccacatcgcc gtaccctcga agatgtactc cgctctcccc tccaacccgc
      961 cagccgtcac gaatagaatc cctcccacgg ccagcgatac cagtagggcc gcaccgaccc
     1021 cgtaccacac ctgcctgatg tcctcgcggc gttcgatctt gacgagatag gcgagcacta
     1081 tcgcgaccaa aagcgttacc tcgaatccct ccctcaaaag ggtaacgaaa gaactaaaca
     1141 ccgagacctc gcctcctcgt tcgccgatac tccttacaaa tgacaatcat ttgcaataac
     1201 tgtgtggcag catagtgagg gcagattgca gagtcaaggg accggcggcc tgggtgtgag
     1261 ctaggtcaaa tggccttgtc gagcggtggg gtaaggctgg ctgcggtcag gcgtggattc
     1321 tgagcctctg cacgacagcg tcggccatct catctgtcgt ggccgtgccg ccgaggtcgc
     1381 tggtcttggt gcctgatgcc agggtctctt cgagtgcgga gaggatacgg gcgcttgcgt
     1441 ctgggtatcc tgcgtggtcg agcatcatgg cgccggccca gatcgcgcct gatgggtttg
     1501 cggcgccctg gcccgcgata tcgggggccg agccgtgaat aggctcgaac agggagggat
     1561 atgttccttc tgggttcagg ttggctgagg gtgcgatccc gatggcccct gcgatggcgg
     1621 ctgtcacctc agagagcaga tcaccgaaga ggttggagcc gacgacgacg tcgaaggagt
     1681 gcggggcgag cacgagcctg gcggccaggg cgtcggcgtg catcagggac gcctctacgt
     1741 cagggtagtc agaggatatc tcgcggaaga tctcatcgaa gaaaggcatc gtaacgctga
     1801 ttccgttcga cttggtcgcc ccgaccagaa ggccacgtct ggccttcgcc tcctcgaaag
     1861 cgtaacggat tatcctctcc acgccgcgtc gagtaaagat gctctcctga acggcgatct
     1921 catgtggcgt acccctgtac acccgcccgc ccgagtcaga atactctccc tcagtgttct
     1981 ccctgacgac ggtgatgtcg aggtcgttcg gctccgccag cgggctcttc acgccgcgca
     2041 aaaggcgtgc ggggcgtaga tttacatact ggtcgaaggt gcgccttata ggaagcaaga
     2101 gtccccagag ggagaggtga tccggcaccg taggccagcc gacggcgccg aggtagatct
     2161 catcgaaggc cgcaagggta tcgaggccgt cctccggcat catccgcccg tgctccaggt
     2221 ag
//
LOCUS       DASTKM010000087        14588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1076835, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000087 DASTKM010000000
VERSION     DASTKM010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14588
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076835"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFQ10_03290"
     CDS             <1..180
                     /locus_tag="VFQ10_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964396.1"
                     /translation="AEILEQLKRYFPDALWPCVRYNSKLSEAPGFGQHIFEYSKRSNG
                     AADYARLAERLAKGT"
     gene            189..830
                     /locus_tag="VFQ10_03295"
     CDS             189..830
                     /locus_tag="VFQ10_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964397.1"
                     /translation="MGKTRKVPEDLMGSILREISGPPGGERDSQESVEPGTAPPASGG
                     ESAISWEDQPWLVGTTFNLSKQVGRELDRLRHDLQRQEGIHTSNSEIAEVALRIAIED
                     ARERGRESELARRLVSGLGGRPVYRTGGAGLATRRSVDGSGRILETTYNEEGEVVDEE
                     EVGDVTDLPVETEYVDAQGRLRSLARDELGNTFEWVTAEGLGTLEARIVRAAD"
     gene            905..1447
                     /locus_tag="VFQ10_03300"
     CDS             905..1447
                     /locus_tag="VFQ10_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964398.1"
                     /translation="MSIPRYYTVGAVARDVRALKALDERLEDLGVTADPLLVFSRRKD
                     ERLVGVTLPDVRTRRVESGLSRTQWFELASTYLGVTAVSVLMGAFHPPTGIAVQAVIT
                     LAVIIGLILYHRRPHLQKKLLAMGLPERFAEEWEGAFHDGFALALATVPSDLFDEMQD
                     AFLQAPGIESPFALDRRPVL"
     gene            1640..2335
                     /locus_tag="VFQ10_03305"
     CDS             1640..2335
                     /locus_tag="VFQ10_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HET9964399.1"
                     /translation="MIVRERPDSFVLIEQHEHALIAGEFALRWKERPRPFESTVYAVA
                     NHDLAWRELDREVLWNEEKDRPYSFVDYPLDLKLPAQRRGIDLVADEDPYAGCLCSMH
                     YARFLLDSDRPEEVEFRDGEVERQGSLEVRMSENELENLERNFRLLRLCDGLSLFLCL
                     NEPCGEDSPPPYPGGFAFEGTRFGPVWDDGQTLRLDPNPFSEAFGIEIPYRIIGRDRR
                     PLGSDVLELQVTC"
     gene            2391..3449
                     /locus_tag="VFQ10_03310"
     CDS             2391..3449
                     /locus_tag="VFQ10_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET9964400.1"
                     /translation="MRNASYEFLKRLLSTPGPSGYEEAAAKVWREEAEKFAEVGGDRM
                     GNSFATIGAGGSPKVMLAGHVDEIGVMITHIEENGLLRFTGVGGWDPQVLVGQRIRLL
                     AKGGDLSGVIGKKAIHLMEPDERKKVSEIKSLWIDIGAKDAEEAGGMVRVGDVGVIDQ
                     DIVELPNGRIASRSVDNRMGAFVVLEALRLLSEDDDLGAEVVAVACVQEEIGLYGARG
                     AAFGLDPDAAIAVDVTHATDTPGVSKSEHGDHSLGGGPVIKRATNISPIVSEGLIAAA
                     EGENIPYTLEADSRSTGTDADAIQFTRAGIATGLVSCPNRYMHSPNEVVDVEDVENCA
                     RVISAYIKTLSPETNFIR"
     gene            3463..4356
                     /locus_tag="VFQ10_03315"
     CDS             3463..4356
                     /locus_tag="VFQ10_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9964401.1"
                     /translation="MTTEEEPKRRASIGVEAALLLTVFFFGTNFTAVKIVVESVSPIL
                     FAATRFTLAGCLLLLFVPFLEGGSRLRREDLLAVLGLGVVGVTLTQTVFTIGVSLTTA
                     ANTALVYSTAPVWGMLLGFALGLERPRLAGILGVGLSLLGVGFIVYGGLEFAGTSLVG
                     DALILVAAVFWGSYTVLSLSLLERYSPIALAAYAMTLGGLAAFPLAALDPGGFDIGSL
                     DGLAWGAAAYSALCSSAFGFAAWGYGVSHVGANRVLIYQYLVTLTGVSTGIVLLGEDF
                     GLEQLVGAVVILCGVYLARRR"
     gene            complement(4380..5075)
                     /locus_tag="VFQ10_03320"
     CDS             complement(4380..5075)
                     /locus_tag="VFQ10_03320"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET9964402.1"
                     /translation="MWGRHQEEGGFPVTTSGGPDSDTGRIEAFSDGVFAIAITLLIIE
                     IGVPHVVGTASLFEKLVELWPSYLGYAISFLVIGTVWANHHNRFRLISRSDHILLFLN
                     IVFLMCVAFIPFPTALLADYLSGTNEHRTTAVAVYSGTLAVTAIFFTILWLYAAGNYR
                     LVDRAVDPHLLRAMTRRYVFGMILYILAFALAFVSPAASLALIVILALIFVLPEPEGP
                     GSSRRTRRRPIRG"
     gene            complement(5195..6289)
                     /locus_tag="VFQ10_03325"
     CDS             complement(5195..6289)
                     /locus_tag="VFQ10_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9964403.1"
                     /translation="MKGHALQRYFLLALMALAALAFVLGVIYLDPGTGRTQTADPVES
                     APDGIPADEPVARVASVLSPSVVQINVSGIEQTPFGAQKQEGLGSGVIYRSDGYIITN
                     NHVVEGSRDVEVAFADGTTERGEVVGTDPTTDIALVSVDRNDLPAADFASGDPIVGQT
                     AVAVGSPSGFESTVTSGIISGTGREVPAEYTGGTQAPSLVDLIQTDAAISPGNSGGAL
                     ADREGRVMGINVAYLPPAETGAENIGFAIPSATAVSVADQLIEDGEAVHPYVGVYLSD
                     LTQQTASKFGSPVDSGALIEEVEPGSPADDAGIHRGDIVTAAGSEEVRSSGDLLSALR
                     DYMPGDTVELKVLRNGEKSTLQVNLAERTQ"
     gene            6531..7220
                     /locus_tag="VFQ10_03330"
     CDS             6531..7220
                     /locus_tag="VFQ10_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9964404.1"
                     /translation="MRIMVVEDEERLARLISRVLGEEGYAVETEVNGRQALMRALADE
                     YDLLIVDWMLPELSGVQLVKRLRAAEVGTPVIMLTARDQIEDRVEGLDAGADDYLPKP
                     FAFPELLARVRALARRPWGEEKDGAILGAGDVTLDPVRHVVRHRGETVDLTAKEFALL
                     ATLLQRPGQVFTRPVLLDTVWGASPEVYANVVDLYVSYLRKKLDGDGDTSHIRTIRGV
                     GYTFEPQPGPH"
     gene            7220..8503
                     /locus_tag="VFQ10_03335"
     CDS             7220..8503
                     /locus_tag="VFQ10_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9964405.1"
                     /translation="MFDHIRRRLTVGYVGILALILLVFGVVVVFTFYLRASEQQDNLL
                     LQKAEDKASSVLSGEDKYGVVKATTDYDVAVIVLLPDGSSYGDLDETSFSLGLPYTYM
                     AQRAGQERQTISETVPGPGGAVRVMSIPVQKYGEFGSVVAVVQAAQSRQAVSETVERL
                     IFLLVLTGFGALVLATAGGLFMSRRAMRPVQEAFGRQRTFIADASHELKTPLTLIRAD
                     AEVLSRGIDDPNKISENRELVEDLLGETDRMNAVLSDLLLLARLDAQKVSVSREPFDL
                     ALVISETSERFAARARSEDKNLEVHHSGKLPARGDAPRTGQGLAALLDNALRFTPPGG
                     TITVEGRITGKQVEVTVTDTGPGIAPESLDRIFERFYRADAHSAARSRGLSGGGTGLG
                     LAIARDLARAQGGEVSAENAEGGGARFTLSLPLGP"
     gene            complement(8540..9724)
                     /gene="moeB"
                     /locus_tag="VFQ10_03340"
     CDS             complement(8540..9724)
                     /gene="moeB"
                     /locus_tag="VFQ10_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET9964406.1"
                     /translation="MQTRQPLVESPNGALELSNEEIARYSRHLIMPEVALEGQKQLKA
                     AKVLTVGTGGLGSPLALYLAAAGIGTIGIVDFDVVDESNLQRQIIHGTSDVGRPKVES
                     AHDKIKDINPNVEVRVHEEALTSENALEIIEDYDVIVDGTDNFPTRYLVNDACVLLNK
                     PNVYGSIFRFEGQASVFYAEEGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGAIGTIQ
                     ATETAKLILGIGEPLIGRLLLYDALNMRFREMKLRKDPNCPVCGENPTVTELIDYQEF
                     CGIPQAESAEQVNGVPEISVNELKQKLDHGEEINVLDVREPHEYEVANIGVRLIPLGE
                     LPQRLAELDQDEYFVVHCKTGSRSAKAVKLLQDAGFQNVHNVKGGITAWSEEIDPSVP
                     KY"
     gene            complement(9781..10062)
                     /locus_tag="VFQ10_03345"
     CDS             complement(9781..10062)
                     /locus_tag="VFQ10_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HET9964407.1"
                     /translation="MPKVKIPTPLRQYTGGNTEVEVGGATAGEALENLASEHPDLKQH
                     LYTGDGKLRSFVNVYKDDEDIRYLEGQDTEVTDGDEISIIPSIAGGRCR"
     gene            complement(10075..10494)
                     /locus_tag="VFQ10_03350"
     CDS             complement(10075..10494)
                     /locus_tag="VFQ10_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET9964408.1"
                     /translation="MPLKIGAGDLGQIHDHAKEAYPEECAGALVGIESGGIKVVVDVW
                     RAENTHEEERSRRFLIEPLEVKRFEERADEKDMGLLGFYHSHPDHPAEPSEYDREHAW
                     PGYSYVIASLGAENIEDMRSWTLRDDRSGYDEEPIVG"
     gene            complement(10496..11383)
                     /locus_tag="VFQ10_03355"
     CDS             complement(10496..11383)
                     /locus_tag="VFQ10_03355"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET9964409.1"
                     /translation="MTELVGGTPLLELPGISAEVPGVDILGKAEWYTPGGSVKDRPAL
                     WMIRDGEKRGSLTPEKTILDATSGNTGIAYAWIGAALGYRVKLCMPKNASEERKKILC
                     AYGVDFVLTDPDDGSDGAIREARRLYAEDPERYFYPDQYSNAANPRAHYESTAPEIWE
                     QTNGEVTHFVAGLGTSGTFVGTATRLKEYNPAIEAISFEPDSAFHGLEGMKHMESAIV
                     PAIYDPTIADQNRAARTEDAYEMVKRVAREEGILIGLSAGAAVATALEVAREIESGTV
                     VTILCDGADKYLSESFWEQ"
     gene            complement(11588..12310)
                     /locus_tag="VFQ10_03360"
     CDS             complement(11588..12310)
                     /locus_tag="VFQ10_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET9964410.1"
                     /translation="MTETHTRQQQTTEEKLPAQYINYVFFELDPLWRRLPGEERARGK
                     REFLEAAEIHSGEMLLRTFSLMGLRADADFMLWRIGYDLDAFESMTGALLKTGLGKYL
                     RVTYSYFALSRRSVYVGEHTPGFENRTYIVPGEGEYLFVYPFVKTRAWYRLPIEERQR
                     MMNEHMQIGRRHYPVKNNTGYCFGIDDYEFILSFEAESPEKFQDLMMELRESEASSYT
                     ELDTPIFTCRRRTLPEILQTLG"
     gene            complement(12452..13450)
                     /locus_tag="VFQ10_03365"
     CDS             complement(12452..13450)
                     /locus_tag="VFQ10_03365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9964411.1"
                     /translation="MKYRTLGSTDIAVSEVGFGVWTVSTGWWGEVDDERSVRLLREAR
                     ERGINYFDTADTYGSGKGETLLADAFGHVRDEVVISTKIGYDFYNHTARRGQQERPQD
                     WSEEFIRFALEQSLKRLDTEYIDFLHLHNTKMDAIEDEGLFALMEEFMSEGKIRAYGI
                     ALGPKIGWLEEGVRAMRERRVEGVQMIYNLLEQDPGRGLIEAARDTGTSLIVRVPHSS
                     GMLEGHFDENTTFAKNDHRRHRPKEWLTTGLKKVEQLSFLTESGERTLGQSALKFVLS
                     APEVVSTLPNIYGKEQIEEFAATSETPDLTPEELSRIAELYENDFGISQSAGRHVS"
     gene            13593..14357
                     /locus_tag="VFQ10_03370"
     CDS             13593..14357
                     /locus_tag="VFQ10_03370"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HET9964412.1"
                     /translation="MIARYAGGESGARRAARAGSVVVVVDAFRASTTIAVLVSKGARI
                     IPVASVEGAAAAEADLRIGERGSAKVAGFDFGNSPTEILGSEIPPGSTVALSTTNGTR
                     VLEAARGASTILTGAFVNASILAEELASGTYDGDEVVVVGCGWEGHRASEDEAAAGAI
                     LHRLRSHGAVLDVRARRVVEVYLSRSVGTLRNSSAARRLKCLGYEGDLEFCLAEDTVP
                     VVPRLVGGTFVGLIAGSQRPRRSSARGPTQKAGISS"
BASE COUNT         2613 a         4441 c         4633 g         2901 t
ORIGIN      
        1 gctgagatac tcgagcaact caagcgttat ttccccgacg ccctgtggcc ttgtgtgcgc
       61 tacaactcga agctctcgga agcgccaggg ttcggccagc acatcttcga gtactcgaaa
      121 aggagcaacg gggccgctga ctacgcccgt ctggcggagc ggctcgcgaa agggacgtga
      181 aaggagacat gggcaagaca agaaaagtgc cggaagacct catgggaagc atcttgagag
      241 agatctccgg gccgcctgga ggcgaaaggg attctcagga gtccgtcgag cctggaacgg
      301 ccccgcctgc ttctggtggc gaaagcgcaa tctcctggga ggatcagccc tggctggtgg
      361 gtaccacgtt caacctctcc aaacaggtcg gcagagagct ggacaggctt cggcatgacc
      421 tgcagcgtca ggagggtatt cacacctcca actccgagat cgccgaagtg gcgctcagga
      481 tcgcaatcga agacgccagg gagagaggga gagagagtga gctggccagg agactggtca
      541 gcggactggg cggccggccc gtgtatcgga ccggcggggc aggcctggcg acacggcgca
      601 gcgtcgacgg gtcaggacgc atcctcgaga ccacctacaa cgaagagggg gaggtagtcg
      661 acgaggagga agtcggcgat gtcaccgacc tgccggtgga gacggaatac gtggacgcgc
      721 aggggaggct caggagcctg gcgagggatg agctggggaa caccttcgag tgggttacgg
      781 ccgaggggct cggcacgctc gaggcgagga tcgtccgggc cgccgactga gaggattgcg
      841 tctggacact ccctgaggcc gctgaaccaa gacgggccga aatcactggt aaaatcgccg
      901 aagcatgagc attccacgtt actataccgt cggggccgtc gcgcgggacg tgcgggcgct
      961 caaagccctt gacgaacgtc tagaggacct tggggtcact gctgacccac tcctcgtgtt
     1021 ttcgcggcgc aaggacgaac gactcgtggg cgtaacgctc cctgacgtca gaacccgccg
     1081 ggtcgagtcc gggctctcac ggacgcagtg gttcgagttg gcgagcacct acctcggggt
     1141 gacggccgtg agcgttctca tgggtgcctt ccatccgccg accgggatcg ccgtacaggc
     1201 cgtcattacc ctggccgtta tcatcggcct catcctctac caccgcaggc cgcaccttca
     1261 gaagaagctt ctcgccatgg gcctccccga gaggttcgcc gaggagtggg aaggtgcctt
     1321 ccatgacggc ttcgccctgg cgctggccac ggtaccctca gacctcttcg acgagatgca
     1381 agacgctttc ctccaagccc ccggaatcga gtcaccgttc gccttggata ggcggccggt
     1441 gttgtaaagt cggtcagcac gctacgggcc taaaggccct cgctctcagc ttgccagcta
     1501 cttccgttcc ggcctcctcc tgtcgcgtgg gagcaactct cggaggttcc cgtgcctagg
     1561 gcgaggccta ccacctgctg actagctgac aagcggaagc cggaggctgg agcgtgctga
     1621 aagctgctaa gcttggccca tgatcgttcg cgagcggcca gactcttttg tgctcatcga
     1681 gcagcacgag cacgccttga tcgcgggcga gttcgcgctc cggtggaagg agaggcctcg
     1741 acccttcgag tccacagtct acgccgtcgc caaccacgat ctggcctggc gcgaactgga
     1801 ccgggaggtg ttgtggaacg aggagaagga caggccctac tcctttgtgg attatcccct
     1861 ggacctgaaa ctgccggccc agaggagagg gatagatctg gtagcggatg aggaccctta
     1921 cgcgggatgc ctttgcagta tgcactacgc ccgcttcctg ctcgactccg atcggcctga
     1981 ggaggtcgag ttccgcgatg gcgaggtgga gcgccagggc agcctcgaag tgagaatgtc
     2041 cgaaaatgag ctggagaacc tcgagcgaaa cttccgctta ctgcggctct gcgacggcct
     2101 ctcgctgttc ttgtgcctca acgagccctg cggcgaagac agtccgccgc cttatcccgg
     2161 agggttcgcg ttcgaaggga cgcgcttcgg gccggtatgg gatgatggac agacgttgcg
     2221 cctcgatccc aatccctttt cggaggcgtt cgggatagag atcccctacc ggattatcgg
     2281 tagagaccgg cggcccctcg ggagtgatgt gctcgaactt caggtaacct gttaggggcg
     2341 ccgggaggcg ctcattcaga gattcgcttc tactatagaa aggacccatt gtgaggaacg
     2401 cctcgtacga gttcttgaaa aggctgcttt cgacgccggg gcccagcggt tacgaagaag
     2461 ctgccgcgaa agtctggagg gaggaggccg agaagttcgc cgaagtcggg ggcgacagaa
     2521 tggggaacag cttcgccacc ataggggcgg gtggctcacc gaaggtcatg ctcgcagggc
     2581 acgtagacga gatcggggtg atgatcaccc acatagagga gaacggcttg ctccgcttta
     2641 ccggcgtcgg cggctgggat ccgcaggtgc tcgtgggaca gcgcattcgg ttgcttgcga
     2701 agggcggcga tctgtccggt gttattggca agaaggcaat ccaccttatg gagcctgatg
     2761 agcgcaagaa ggtctccgag ataaagagcc tgtggatcga tataggcgcc aaagacgccg
     2821 aagaggccgg gggcatggtc cgcgtcgggg acgtcggcgt aatcgaccag gacatcgtgg
     2881 agcttccgaa cggccgcata gcttcccgct ccgtggataa tcgcatgggc gcgttcgtgg
     2941 tgctagaagc cctgaggttg ctctccgaag atgatgatct cggtgccgag gtcgtggccg
     3001 tggcctgcgt gcaggaggag ataggtctct acggtgcccg tggggctgcc ttcgggctag
     3061 atcccgacgc ggcaatcgcc gtcgacgtga cccacgccac agatactcca ggcgtctcca
     3121 agagcgagca cggggaccac tccctgggcg gtggccccgt tataaagcgg gcgacgaata
     3181 tctcgcccat cgtctcggaa ggcctcatcg cggcggctga gggcgagaat atcccctaca
     3241 cgctggaggc cgactcgcgc tcgacgggga ccgacgcaga cgccatacag ttcaccaggg
     3301 ccgggatcgc gacgggactc gtctcgtgcc ccaaccgcta catgcactct cccaacgagg
     3361 tcgtggacgt agaagacgtc gagaactgcg cccgtgttat ctccgcctac ataaagaccc
     3421 ttagcccgga gacgaacttc atccgttaac cgaaggctcc cgttgaccac tgaagaggaa
     3481 ccgaaacggc gggcctcgat aggagtcgag gccgcgctac tgttgacggt cttcttcttc
     3541 gggaccaact tcacggcggt aaagatcgtc gtcgagagcg tctcgccgat cctcttcgcc
     3601 gcaacacgct tcaccctggc cggttgtctc ttgctcttgt tcgtgccctt tctcgaaggg
     3661 gggagtcgcc tgcgtcgcga ggacttgctc gccgtactcg gcctcggggt ggtcggcgtc
     3721 acactcaccc agacggtctt caccatcggg gtgagcctga ccacggcggc aaacacggcc
     3781 ctcgtctact cgacggcccc cgtgtggggg atgctcctcg gattcgcact cggcctggag
     3841 cggccgaggc tcgctggtat cctcggcgta ggactctccc tcctcggggt cgggttcatc
     3901 gtgtacggcg gactcgagtt cgcaggcacc agcctcgtcg gggacgctct catactggtc
     3961 gctgccgtgt tctggggctc ctacaccgtc ctctcgctct cacttctgga acgctactct
     4021 cccattgccc tcgccgccta cgccatgacc ctcggcgggc tcgcggcctt cccgctcgca
     4081 gcgctcgacc ctggcggttt cgacatcggt tccctggacg gtctcgcgtg gggggcggcg
     4141 gcttactctg ctctgtgttc ctcagccttc ggcttcgccg cctggggtta tggagtctcc
     4201 cacgtcgggg cgaaccgggt cctgatctac cagtacctcg tcaccctgac aggcgtgagt
     4261 acgggcatcg tactcctcgg cgaggacttc ggactggaac aactcgtcgg cgcggtcgtc
     4321 atcctctgcg gggtctacct ggcgagaaga agatagcgag gatctccgga ccttccggtt
     4381 cagcctcgaa ttggtcgccg ccgggtgcgg cgtgagcttc ctggtccttc cggttcgggt
     4441 aagacgaaga tcagggcgag gatgacgatg agcgccagac tcgccgccgg gctgacgaag
     4501 gcaagcgcga aggccaggat gtacagaatc attccgaaca cgtagcgacg cgtcatggca
     4561 cgcagcaagt gagggtccac cgccctgtcc acgaggcggt agttccctgc ggcgtagagc
     4621 cagagaatgg tgaagaagat ggcggtcaca gccagcgtgc cgctgtatac ggcgacggcg
     4681 gtagtccggt gctcattggt tcctgacaga taatcggcga gcagggccgt ggggaagggg
     4741 ataaaggcga cgcacatcaa gaacacgatg ttcaggaaga gcaggatgtg gtctgagcgt
     4801 gagatcaggc ggaaacggtt gtgatggttc gcccagacgg tgccgattac gaggaagctg
     4861 atcgcgtacc ctaggtaaga aggccacaac tcgacgagct tctcgaaaag agatgccgtg
     4921 cccaccacgt gcggaacccc gatctctatg ataaggagcg tgatcgcgat ggcgaacacc
     4981 ccgtcggaga aggcctctat tcttcctgta tcactatccg gcccgccgct tgtagtaacc
     5041 gggaatcccc cttcttcctg atgcctgccc cacagtgtgc actacctgtg agtccgagcg
     5101 caatgcacgg gcgccctccc tctcgaggac gcccgtttgt ccgcaagctc cggtctgttc
     5161 accggcatcg tctagccggt ggggagcaag cgccttactg ggtacgctcc gcgaggttta
     5221 cctggagcgt ggacttttcg ccgttcctga ggactttcag ctctaccgta tcgcccggca
     5281 tgtagtcgcg cagcgccgag agcaggtcgc ccgagctacg gacctcctcc gaacctgcgg
     5341 cggtcacgat gtcaccgcga tgtattccgg cgtcatcggc cgggctacca ggctcgacct
     5401 cttcgatcag agcccctgag tctacggggc ttccgaactt gctcgcggtc tgttgggtga
     5461 ggtcagagag gtagacgccg acgtaagggt ggacagcctc gccgtcctcg atcagctggt
     5521 ccgccacgga gacagctgtg gcagaaggta tggcgaagcc gatgttctca gcacccgtct
     5581 cggccggtgg caggtaggcc acgtttatcc ccatgaccct gccttcgcgg tctgccaggg
     5641 cgccgcccga gttgcctggg gagatggctg cgtcggtctg gatgaggtcg accagggagg
     5701 gggcctgcgt accgcctgtg tactcggcgg gtacctcacg ccctgtgccg ctgataatgc
     5761 ctgaggtgac cgtagactcg aagcctgatg ggctgccgac agcgaccgcc gtctggccga
     5821 ctatcgggtc cccgctcgcg aagtccgccg cgggcaggtc gttgcggtcc acgctgacga
     5881 gggcgatgtc cgtcgtcggg tccgttccta cgacctctcc gcgctctgtg gtgccgtcag
     5941 cgaaggcgac ctcgacgtcg cgcgagccct ctacgacatg gttgttggtg atgatgtagc
     6001 cgtcggagcg gtagatcaca ccgctcccta gcccctcttg cttctgcgcc ccgaaagggg
     6061 tctgttcgat tcctgagacg ttgatctgca ccacggaagg gctcagtacg gaggcgaccc
     6121 ttgccacggg ttcgtccgcc ggaatgccgt ccggggccga ctcgacggga tcagcagtct
     6181 gcgtcctgcc cgtgccaggg tcgaggtaga tgacgccaag cacgaaagcc agcgccgcca
     6241 gtgccatgag agccaacaag aaatagcgct gcaaggcgtg gcccttcata ccgttacctc
     6301 cccgctctct cgcggccccg actccagaag cgcctcgaga agtcgccctg cgctgtaccc
     6361 gcctgacagc tttctcggat gagttaccaa ttctcctcct tgtggtgtga tctctgcgtc
     6421 gtagtctgga cttccaacat gtgaagagcc tttgcggtcc atgagcgttt tcacagaaaa
     6481 cgtcgcacag gggggcagat ctgctgcaga gcggcgataa tacatgtgtg atgcggataa
     6541 tggtagtaga ggacgaagag aggctggcgc gcctgatctc gcgggtcctt ggcgaggagg
     6601 gctacgccgt cgagacggag gtcaacggcc ggcaggcgct catgcgggca ttggcagacg
     6661 aatacgacct ccttatcgtg gactggatgc tcccggagct gagcggcgtc cagctggtca
     6721 agaggctaag ggccgcggag gtagggacgc cagtgatcat gctgaccgcc cgcgaccaga
     6781 tcgaagaccg cgtggaaggc ctcgacgccg gtgccgacga ttacctcccc aagcctttcg
     6841 ctttccccga actgctcgcc cgcgttcggg ctctcgcacg ccgtccgtgg ggcgaggaga
     6901 aagacggcgc gatcctcggg gccggcgacg ttacgctcga tcctgtgcgg cacgtggttc
     6961 gccacagagg tgagaccgtg gacctgacgg ccaaggagtt cgcactgctc gccacccttc
     7021 tccagcgccc cgggcaggtc ttcacccgcc cggttctgct cgataccgtg tggggcgcct
     7081 cgcccgaagt ctacgccaac gtcgtcgacc tctacgtctc ctacctgcgc aagaaactcg
     7141 acggcgacgg cgacacatcc cacatccgaa ccatccgcgg tgtcggctac accttcgagc
     7201 cgcaaccagg accgcactga tgtttgacca catcaggcgg cgcctgacgg tgggctacgt
     7261 aggcatactg gccctcattc tgttggtctt cggcgtcgtt gtggtgttca ccttctacct
     7321 gcgggcaagc gaacaacaag ataatctact ccttcagaag gcggaagaca aggccagcag
     7381 cgtattgagc ggcgaagaca agtacggtgt cgtgaaggct accactgatt acgacgtggc
     7441 cgtgatcgta cttctgcctg atggttcgtc ctacggagat ctggacgaga cgtccttttc
     7501 tctcggcctc ccctacacat acatggccca gagagccggg caggagaggc agacgataag
     7561 tgagaccgtt cccgggcctg gtggcgccgt aagggtgatg agcattcccg tacagaaata
     7621 cggagagttc ggaagtgtgg tcgcggtggt acaggcagct cagtcgcgtc aggcggtgtc
     7681 tgaaacggtg gaacggctga tcttcttgct ggtcctcacg ggctttggag cgttggtact
     7741 ggcgacggcc ggcgggcttt tcatgtcgcg gcgggcgatg cgtccggtcc aggaagcgtt
     7801 cggcaggcag aggacattta tcgctgatgc ctcccatgag ttgaagacgc cgctgaccct
     7861 gatccgggcc gacgccgagg tcctctcccg cggtatcgac gacccgaata agatctctga
     7921 gaaccgtgag ctcgtcgagg acctgctcgg tgagaccgac aggatgaacg ccgtgctctc
     7981 cgacctccta ctgctggcgc gtctggatgc gcagaaggtt tccgtctccc gcgaaccttt
     8041 cgacctcgca ctagtcatct ccgagacctc ggagcggttc gccgcccgcg ccaggtccga
     8101 ggacaagaac ctcgaagttc accactcggg caaacttcct gcgcgcggcg acgcgccccg
     8161 tacgggccag ggcctcgccg ccctcctcga taacgccctc cgcttcacac ctcccggcgg
     8221 gaccataaca gtggagggcc gtatcacagg caaacaggtc gaggtgaccg ttacggacac
     8281 cgggccaggg atagcccctg agagcctaga tcgcatcttc gagcgtttct acagggcaga
     8341 cgcgcacagc gccgccagga gcaggggact ctcgggcggc ggaacgggtc tcggtctcgc
     8401 catcgcccgc gacctcgccc gcgcccaggg cggtgaagtc agcgccgaaa atgccgaagg
     8461 aggaggggca aggttcacgc taagcctccc tctcggtccc tgaacccaaa ctcgcgccag
     8521 gtcgctcgca gtaccctccc taatacttcg ggacgctcgg gtcgatctcc tcgctccagg
     8581 ctgtgatgcc gcccttgacg ttgtggacgt tctggaagcc ggcgtcctgc aggagcttga
     8641 cggccttcgc gctgcggctg ccggtcttgc aatgaacgac gaagtactcg tcctgatcca
     8701 gctccgccag acgttgtgga agctccccca agggtattag cctcacacct atgttggcga
     8761 cctcgtactc gtgcggctcg cgtacatcga gcacgtttat ctcctcgcca tgatccaact
     8821 tctgcttgag ttcgttcacg ctgatctcag gcacgccgtt gacctgctcg gcactctcag
     8881 cctgcgggat gccgcagaac tcctggtagt cgatcagctc cgtaacggtc gggttctcgc
     8941 cgcagacggg gcagttcggg tccttgcgga gcttcatctc gcggaagcgc atgttcagcg
     9001 cgtcgtacag gaggagcctt cctatgagcg gctcgccgat cccgagtatg agcttggccg
     9061 tctctgtggc ctggatggtg ccgatggcgc caggcaggat gccgagcact ccgccctcgg
     9121 cgcagctcgg gacgaggccg ggaggtggtg gctcggggta gaggcagcgg tagcaaggac
     9181 cttcctcggc gtagaacacg ctcgcctggc cctcgaagcg gaagatcgaa ccgtagacgt
     9241 tgggcttgtt cagtaggacg caggcgtcgt tcaccaggta cctggtcggg aagttgtccg
     9301 tgccgtcgac gatcacgtcg tagtcctcga tgatctccaa cgcgttctcg ctggtgagcg
     9361 cctcctcgtg gaccctgacc tcgacgttgg ggtttatgtc cttgatctta tcgtgggcgc
     9421 tctccacttt gggacgtccc acgtccgaag tgccgtggat gatctggcgc tgcaagttcg
     9481 actcgtcgac aacgtcgaag tcaacgatcc ctatggtccc gattccggcc gcagccaggt
     9541 aaagggcgag cgggctcccg agtccgcccg tgcctaccgt gagtaccttc gcggccttga
     9601 gctgtttctg cccttcgagg gcgacctcgg gcatgatcaa gtgcctgctg tagcgtgcga
     9661 tctcttcgtt gcttagctcg agtgccccgt tgggcgactc cactaatggc tgtcttgtct
     9721 gcatctgaag ataaacctct cgtttgaaaa agtaacgatc gaagggtgga tccttgaaaa
     9781 ctacctacat cgtcctccgg cgatggaggg gattatagaa atctcatcgc catcggtgac
     9841 ctcggtgtcc tggccttcga ggtaacggat gtcctcgtcg tccttgtaga cgttgacgaa
     9901 cgagcgcagc ttgccgtcac ccgtgtacag atgctgcttg aggtcggggt gctctgaggc
     9961 aaggttctcg agggcttcgc ccgccgtggc gccgccgacc tcgacctcgg tgttaccgcc
    10021 ggtgtactgt ctcaaagggg ttgggatctt gaccttgggc atatctgagt ctccttatcc
    10081 tactattggc tcttcgtcgt aacccgagcg gtcgtctctt agggtccacg agcgcatgtc
    10141 ttctatgttc tcagcgccca gcgaggctat cacgtaggag tagccgggcc aggcgtgctc
    10201 gcggtcgtac tcggaaggtt ctgctggatg gtcagggtgt gaatggtaga agccgagcaa
    10261 ccccatgtcc ttctcgtcgg cccgctcctc gaatctcttt acctcgagcg gctctatgag
    10321 aaagcggcgg ctgcgctcct cctcgtgcgt gttctcggcc cgccacacgt cgacgacgac
    10381 cttgatgccg ccgctttcga tgccgaccag cgcaccggca cactcctcag ggtacgcctc
    10441 tttggcgtgg tcgtggatct gccccaggtc ccctgcgcct atcttgagcg gcaacttact
    10501 gctcccagaa gctctcggag aggtacttgt cggcaccgtc gcaaaggata gtgacgaccg
    10561 tacccgactc tatctccctc gcgacctcga gcgcagtcgc gaccgccgcg ccggccgaga
    10621 ggccgatgag gattccttcc tcgcgcgcaa cccgcttgac catctcgtag gcgtcctcgg
    10681 ttctcgccgc ccgattctgg tcggcgatgg tcgggtcgta gatggcgggc acgatggcgc
    10741 tctccatgtg cttcatgcct tcgaggccgt ggaaggccga gtcgggttcg aaggaaatgg
    10801 cctctattgc tgggttgtac tccttgagac gcgtcgccgt accgacgaag gtgccgctcg
    10861 tgccgagccc ggcgacgaag tgcgtgacct cgccgttcgt ctgctcccag atctcaggcg
    10921 ccgtcgattc atagtgcgcg cgcgggttgg cggcgttgga gtactggtcc ggatagaagt
    10981 agcgttcagg gtcctctgcg tagagcctcc gcgcctcacg tatggccccg tccgagccgt
    11041 cgtcagggtc ggtaaggacg aagtcgacgc cgtaggcgca taggatcttt tttcgctcct
    11101 cgctggcgtt cttcggcatg cagagcttca ccctgtagcc gagagcggcg ccgatccaag
    11161 cgtacgctat ccccgtgttt ccgctcgtcg cgtcgaggat tgtcttctca ggcgtcagag
    11221 agcccctctt ctcaccgtct ctgatcatcc acagggcagg ccggtccttg accgatccgc
    11281 cgggcgtgta ccactcagcc ttgcccagga tgtcgacgcc ggggacttcg gccgagatgc
    11341 caggcaactc caagagtgga gtgccgccga caagctccgt cacgttcgtc cccgcccgct
    11401 cattaatccg agtgatcatg tcgggattga ttatacaggg tggagatgca gcgtccagag
    11461 aatcaaggtt agatacctga ccaacgttat agccgagagg caagatagaa gcctcccggc
    11521 tatggttggt gcgttgcgaa ggattatacc tgaatcctga gtagcgattc tacaacgaga
    11581 gccgtgatta tcccaaagtt tgcaaaatct ccggcagggt gcggcgacgg caggtgaaga
    11641 tcggggtatc cagctcggta taggaactgg cttcgctctc cctgagctcc atcatcaggt
    11701 cctggaattt ctcgggggat tcggcctcga aggagaggat gaactcgtag tcgtctatcc
    11761 cgaagcagta gcccgtgttg ttctttacgg ggtagtgtct acggccgatc tgcatatgct
    11821 cgttcatcat gcgctggcgc tcttcgatgg gcaacctgta ccaagcccgc gtcttgacga
    11881 agggatagac gaagaggtac tcgccttctc ccggcactat gtatgtgcgg ttctcgaaac
    11941 ctggcgtatg ctcgccgacg tagacagagc gcctgctgag ggcgaagtaa gaataggtga
    12001 cgcgcaggta cttcccgagt cccgtcttca ggagggcgcc ggtcatgctc tcgaaagcgt
    12061 cgaggtcgta accgatcctc cacaacataa agtccgcgtc tgcgcggagg cccataagcg
    12121 agaaagtgcg caagagcatc tcgccagaat gtatctccgc agcttccagg aactcccgct
    12181 tgcccctcgc gcgctcctcg ccggggaggc gtcgccagag gggatccaac tcgaagaaca
    12241 cgtagtttat gtactgggcc ggaagcttct cttctgtggt ctgttgctga cgggtgtggg
    12301 tttcggtcat ttcgactccc ttcggtcgtc ttagcatttc agcaattcag cacgcgcccg
    12361 acagggcgct tgtcagcatt tcagctttcg cttgcgcaac tccttgctga attgctgaga
    12421 gccgcgaagc cggagcgtgc tgacatgctg actagctgac atgcctacca gctgactggc
    12481 tgataccgaa gtcgttctcg taaagttcag cgatgcggga gagctcttcg ggcgtaaggt
    12541 ccggtgtctc cgaggtagct gcgaactcct ctatctgctc cttgccgtag atattcggga
    12601 gggtggagac tacttcgggg gcggagagga cgaacttgag ggcggactgg ccgagggtac
    12661 gctcgccgga ttcggttagg aaggagagtt gctccacctt cttcaggccc gtcgttagcc
    12721 actctttcgg gcggtggcgg cgatggtcgt tctttgcgaa ggtcgtgttc tcgtcgaagt
    12781 ggccttctag catccctgag gagtggggga cgcgtacgat cagggacgtg cccgtatctc
    12841 tggcggcctc gatcaggcct cggccagggt cctgttcgag gaggttgtag atcatctgca
    12901 cgccttctac cctgcgctcg cgcatcgccc ttacgccttc ttcgagccat cctatcttgg
    12961 gcccgagggc gatgccgtag gccctgatct taccctcgct catgaactct tccatgaggg
    13021 cgaagagccc ttcgtcctcg atggcgtcca tcttggtgtt gtgtaggtgg aggaagtcta
    13081 tatattccgt gtcgagcctc ttcagactct gctccaacgc gaagcggata aactcttccg
    13141 accagtcctg cgggcgctcc tgctggccgc gccgggccgt atggttgtag aagtcgtagc
    13201 cgatcttggt cgagatcacc acctcgtcgc gcacgtgccc gaaggcgtct gcaaggagcg
    13261 tctctccttt gcctgatccg taggtatccg cggtgtcgaa gtagtttatg cctcgctcgc
    13321 gggcttctct gagaagcctt acggacctct cgtcgtccac ctctccccac cagccggtcg
    13381 agaccgtcca caccccgaag ccgacctcgg agaccgctat atcggtactg cccagcgtac
    13441 ggtatttcat cttccgctcc ctgttcgcgt tggaccttgg atcaagctcc attgatctac
    13501 tacattctaa cgtgggacgc tgttgacttt ctctcaatct gcacgcgggt ctataatctc
    13561 ccgatgctgg agaaggctct ggacctcgcg ccgtgatcgc ccgctacgca ggcggggaga
    13621 gcggggcgcg gagggcggcc cgcgccggtt ctgtggtggt cgtggtcgac gccttcaggg
    13681 cgagcacgac gatagcggtc ctcgtctcca agggcgcaag gatcataccc gtcgcttctg
    13741 tcgaaggggc tgcggctgcc gaggcggacc tccgcatagg ggagcggggc agcgcgaagg
    13801 tggcaggctt cgactttggt aactcgccta cggagatcct gggttcggag ataccgcccg
    13861 gctccacggt cgccctctcg accacgaacg ggacccgcgt cttggaggcc gcccgcggtg
    13921 cgagcaccat cctgaccggc gccttcgtca acgctagcat cctggcggag gagctggcca
    13981 gcgggaccta cgacggcgac gaggtggtgg ttgtcggatg tggctgggag ggtcaccgcg
    14041 cctccgagga cgaggcagcg gcaggggcca ttctccaccg gctgcggtct catggtgcgg
    14101 tacttgacgt gcgggccagg cgtgtcgtcg aggtctatct atcgcgttcc gtggggacct
    14161 tgcgcaacag cagtgcggca cggaggctga aatgtctcgg gtacgagggg gatctggagt
    14221 tctgcctcgc cgaggacacg gtgcccgtcg tgcctcgtct cgtcggtggc accttcgtcg
    14281 ggttgattgc agggtcccag cgaccccgcc gatcttcggc ccgggggcca acacaaaaag
    14341 ccgggatttc ttcttgacaa ggggatggcg cgccattacc ctggagtcag cgaaggcgga
    14401 tcaaatgtta caagttccgt cgaatccgcg acgggaagga ggtgagcaga cacgtgacta
    14461 tgactatcac ccacgagaag acccgcgagg ttacccgcga ggacgtggac cgggccatag
    14521 acgtcatcga gcgtcacctc gagaaggagg ccaggacccg caacctgctg gacgagcagg
    14581 cgatcatg
//
LOCUS       DASTKM010000088         9412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_724783, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000088 DASTKM010000000
VERSION     DASTKM010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724783"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /gene="smpB"
                     /locus_tag="VFQ10_03375"
     CDS             complement(<1..446)
                     /gene="smpB"
                     /locus_tag="VFQ10_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET9964413.1"
                     /translation="MTDFARNKKALHDFAIEETYEAGIQLTGPEVKSVREGRANLKES
                     YARVRDGEIFLIGAHISPYANARQERQLPTRDRKLLLHRKEIDRLLGKSQEEGKTLIP
                     LRLYSKNGIVKLQIAVASRKRQYDKRREIAKKTADREIERAMKERLR"
     gene            complement(443..2056)
                     /locus_tag="VFQ10_03380"
     CDS             complement(443..2056)
                     /locus_tag="VFQ10_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HET9964414.1"
                     /translation="MILPAMLVRRGKYSVADPLFVEERRPVLVARKLRRGAEAGDIVL
                     VDAREKNRSLRGEVTRVIGPSEDPRNVYEALFASIGTSREFPRKVDEEAEAVAHREIG
                     DRRDLRHLPTVTIDGADAKDFDDAISVERSAGGYRVWVHIADVTHYVEPGSALDRQAA
                     YRGNSVYLPGTVAPMLPGRLSNDVCSLRPYSERAAVTVEMEVTGEGETRSYSAFRSTI
                     TSDARLTYEAVDAFLRGDGSVERADLVRTAYDLSRKLKLRAARRGKLDLGGREPEYMI
                     DEDGVPMGAGVRRSTPARELIEELMVLANESVAKMLHGKKGGVFRVHERPDQESLGLL
                     TERLAAIGVQAEPTPENLGTISQMAKSDAVNYLILRSIPRALYSPEDLGHYGLALEHY
                     THFTSPIRRYADVLVHRAMLGDEPPEDLAGVAGHISDREYTSMICERTADEYTLMWLM
                     RDRVGEILEGTVVSTAVFGLFVELETGATGLVHVSKLPGWWELEPNGVVLSNDAIGAS
                     YRVGDRVLVELLDVLPLMQRAELRVVKRL"
     gene            complement(2295..3179)
                     /locus_tag="VFQ10_03385"
     CDS             complement(2295..3179)
                     /locus_tag="VFQ10_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HET9964415.1"
                     /translation="MRFNLGFFLAEALKNLRLNLLMSITAVTTTFICILVFGLGLLVS
                     AHIQSIIGSVREDVSVEAFMPNATSQEVDALANEVESWPEVAIVTQISEEQAFAEFKD
                     KFEDQPDLYKDLDLGVFPPSIQIQLEDPDSASAVAERLKEQGFTERNLSYPQQTIDRL
                     NSITSYVIWGLYGATLLFLVASVLLISNAIRLSIFARRKEIEVMKLVGASDGFVRTPF
                     VFEGLLQAFAGASLAAFTVIWINLLFIDWSHNALPYVPISNDAVNTFSTLLLLIAVGV
                     GIGVIGSFLSVTRFLKKI"
     gene            complement(3169..3855)
                     /gene="ftsE"
                     /locus_tag="VFQ10_03390"
     CDS             complement(3169..3855)
                     /gene="ftsE"
                     /locus_tag="VFQ10_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET9964416.1"
                     /translation="MIRFDNVTKVYGNDTIALERVNLGIDSGEFVFLVGASGSGKSTI
                     VRLILKEMEPSAGAIYVNGTRLSSVPRRRIPRLRRDIGCVFQDFKLLPNKTAAENVAY
                     AMEVTGQRRRTIRTKVPQILNLVGLSEKMHKFPDMLSGGEQQRVSIARAFVSQPPILV
                     ADEPTGNLDPTTSVSIMQLLHRINRIGTTVLVATHDREMVDVMRKRVVALEEGRVVRD
                     KVKGAYADEV"
     gene            complement(3934..4656)
                     /locus_tag="VFQ10_03395"
     CDS             complement(3934..4656)
                     /locus_tag="VFQ10_03395"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HET9964417.1"
                     /translation="MRSVVAGVLLPVLVVVLIGPYTFLPSLLEYAVARDVKARLGIEK
                     QPYVELKSDPPVRMLAGEFSGGRIVMKNTDLGNVTATRARIDLDPFDIDVWATMRKGQ
                     VVDRDPLSGDVRVDVPEWEVSRLAKAGSEIPIQDVDVQKDGVVVRSEVSVLGRRFPAS
                     IEGEPGLRDGELIFQPQGLTAAGVQVPDELADRLLAGTSFEYPLDRLPYQTTITGVEA
                     EEGRIILSGSVPSIPLGAYPGG"
     gene            complement(4661..5752)
                     /gene="prfB"
                     /locus_tag="VFQ10_03400"
     CDS             complement(4661..5752)
                     /gene="prfB"
                     /locus_tag="VFQ10_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET9964418.1"
                     /translation="MTELKDKTYELEERLAELEAFFDVDRLRTEAEELGKDMSRPGFW
                     DDPQEARTVSARFSRVKGRVDLLDGLKESLSDSEELLELAEEDGDLLPEVREELRRVE
                     RVLEEQEVARLFAGEYDEGDAILTINSGAGGVDSQDWAEMLARMYQRWAQRRGFGLEV
                     IEYTEGEEAGIKSATFTVSGEYAFGLLSAERGVHRLVRISTFDAGARRHTSFASVAVA
                     PVIGETVEVEIEEKDLKVDTYRASGAGGQHVNKTDSAVRITHLPTGIVAQCQNERSQH
                     QNREVAMRVLRARLFELEREKREQEIAAQSGEKADIEWGSQIRSYVLHPYKLVKDHRT
                     GEETANVDRVLDGDLDAFVYAYLKNRTVA"
     gene            complement(5752..8337)
                     /gene="secA"
                     /locus_tag="VFQ10_03405"
     CDS             complement(5752..8337)
                     /gene="secA"
                     /locus_tag="VFQ10_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET9964419.1"
                     /translation="MANLLTKILRMGEGRKVKVLQQRVEAVSALEPEIEKLSDSALRE
                     KTDEFRERLAGGETIDDLLPEAFAVVREVSRRTLGMRPFDEQIMGGIVLHEGKIAEMK
                     TGEGKTLAATMPVYLNALEGKGVHVVTVNDYLARRDAAWMGEIYTFLGLEVGLIQESM
                     DFAERKIAYHSDVTYGTNAQFGFDYLRDNIATSPDQLVQRELNFGIVDEVDSILVDEA
                     RTPLIISGMPETAADVYYRFAAIVPRLKSGEDYEVDEKKQQVAPTESGVEKVEKALGI
                     ENLYDDVNTNLVNHLNQALRAQTLFHKDDEYIVTDGQVYIVDEFTGRVLEGRRYSEGL
                     HQAIEAKEGVEIKEENQTVATITIQNFFRMYDKLSGMTGTAATEADEFMHTYKMEVVS
                     IPTHKEMIRDDKDDLVYKTEKSKHKAVVEDIVARNKQGQPILVGTVSVDVSERLSALL
                     GRRGIQHNVLNAKQHEREAEIIAEAGERGAVTIATNMAGRGTDIKLGEGVPELGGLYV
                     LGTERHEARRIDNQLRGRSGRQGDPGESRFYLSFEDELLRLFGGQRMQGIIERIGLEE
                     DVPIEAGMISNSVRRAQEQVESRNFQIRKRILEYDDVLNKQREVIYAIRRDILMGEDV
                     DTKSYVEDVLTEVVSEYVSENVYPEDWDLEALTTEINRFYPSRIDFESIDVETLTAAE
                     VLAMVLEDADERLEERKAEWDERTAELEKRGLARGDGVATFEEAERRTLLSVVDSRWR
                     EHLYEMDYLREGIGWRGLSQRDPLVEYKREGFDIFQEMERGLKADYVTYIYRVENIKL
                     REEEMQQLSYSGGEEPNQRPKSLRKTASKVGRNEPCPCGSGKKYKKCHGQPGAPPLGS
                     EARAG"
     gene            complement(8371..8943)
                     /gene="raiA"
                     /locus_tag="VFQ10_03410"
     CDS             complement(8371..8943)
                     /gene="raiA"
                     /locus_tag="VFQ10_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET9964420.1"
                     /translation="MHILIKGRNIPVTEALERYAWEKVERVARFFDDERTASRAEVEL
                     IHERNRAVSEPEVAEATLFINGSVLKASEASEDMYASIDRMSDKLERQVKRFRGRQID
                     RWQGQLKNTADVAPDEAKPFVVEEEEEIETRIVRTKQFQMKPMGAEEAVLQLELLDHD
                     FYVFTSADTGDINVVYRRRDGDYGLIEPAR"
     gene            complement(9163..>9412)
                     /locus_tag="VFQ10_03415"
     CDS             complement(9163..>9412)
                     /locus_tag="VFQ10_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964421.1"
                     /translation="KMKATVSDTLEVVRSTRDQVELSAAQRRANVAGAYTATKPLRGK
                     ILLIDDVFTTGATMSACAGTMVRAGAEEVHALSLCRTC"
BASE COUNT         1570 a         3012 c         2799 g         2031 t
ORIGIN      
        1 cggaggcgct ctttcatggc gcgttcgatc tcgcggtcag ccgtcttttt ggcgatctca
       61 cgccgcttgt cgtactgcct cttgcggctt gcgacggcga tttggagctt gacgatgccg
      121 ttcttggagt agagtctgag ggggatgagg gtctttccct cctcctgact cttgccgagg
      181 aggcggtcga tctcctttct gtgcaacagt agcttgcggt cgcgcgtggg gagttgccgt
      241 tcctgcctgg cgttcgcgta aggcgagatg tgcgccccga tcaggaagat ttctccgtcc
      301 ctcaccctgg catagctctc cttgaggttg gcccgccctt cccttacgga ctttacttcg
      361 ggccctgtca gctggatgcc tgcctcatag gtctcctcga tggcaaagtc gtggagggct
      421 ttcttgttac gcgcgaagtc tgtcacaatc gtttcaccac cctcaactcg gcccgctgca
      481 tgagcggtag gacgtcgagc aactctacga gaaccctgtc ccccaccctg tatgaggcgc
      541 ctatggcatc gttggagagg acgacgccgt tcggctccag ctcccaccat ccagggagtt
      601 tgctcacgtg gacgaggcct gtggcgcccg tctcgagctc gacgaagagg ccgaagacgg
      661 cggtgctcac gacggtccct tcgaggatct cccccactcg gtcccgcatc aaccacatga
      721 gcgtgtactc gtccgcggtg cgttcgcaga tcatggatgt gtactctcta tcagagatgt
      781 ggcccgcgac cccggcgagg tcctcgggag gctcgtctcc cagcatggcg cggtggacga
      841 ggacgtcggc gtagcgccgg atcggggacg tgaagtgcgt gtagtgctcc agcgcgaggc
      901 cgtagtgtcc gaggtcctcg ggcgaataga gggcgcgagg gatggagcgt aggatcaggt
      961 agttcaccgc gtctgacttg gccatctggg agatcgtccc aaggttttca ggcgtcggct
     1021 cggcctgcac accgatggcg gcaagcctct ctgtgagcag tcccagggac tcctggtcgg
     1081 gtctctcatg caccctgaag acgccgccct tcttcccgtg cagcatcttc gcgaccgact
     1141 cgttggcgag caccatgagc tcctcgatca actctctggc cggcgtggag cggcggacgc
     1201 cagcacccat gggcaccccg tcttcgtcga tcatgtactc cggctcccgc ccgccgagat
     1261 ccagctttcc acgtctcgcc gcccgcagct tgagcttgcg cgagagatcg tatgcggtcc
     1321 ttaccaaatc cgccctctcg accgacccat caccgcgcag gaacgcgtcg acggcctcgt
     1381 aggtaagcct cgcgtcgctt gtgatggtgc tccggaaagc actgtagctc ctcgtctctc
     1441 cttcgccagt gacctccatc tctaccgtta cggcggcccg ctctgagtac ggacgtaggg
     1501 agcagacatc gttcgagagg cgcccgggga gcatcggtgc gacggtccct ggaaggtaca
     1561 cagagttgcc cctgtaggcg gcctgcctgt cgagggctga gccaggctcc acgtaatggg
     1621 tcacgtcggc tatgtggacc catactctgt agccgcctgc agaacgctcg accgagatcg
     1681 catcgtcgaa atcttttgcg tcggcgccgt ctatggtaac cgtagggagg tgacgcaggt
     1741 ccctgcggtc cccgatctcc ctgtgcgcga cggcctcagc ctcctcatcg acctttctcg
     1801 ggaactcgcg ggaggtaccg atggaggcga agagcgcctc ataaacgttg cgcgggtcct
     1861 cagaaggccc gatgacgcgt gttacctcgc ccctgagcga gcggttcttc tcgcgggcgt
     1921 ccacgagaac tatatcgcca gcctcagcgc ccctgcgaag cttgcgtgcg accagcactg
     1981 gacgccgctc ctcgacgaag agcgggtctg ctaccgagta cttcccccga cggaccagca
     2041 tagccggaag tatcaagaga gcgctccgat cagaggtatc tggcaaagca caccgtgagt
     2101 ctaccatgcg gcttcgccgc gcatgtcagg acgatcaggc cttgcgtcct tggcttgtca
     2161 gcatttaggg ttcgcggtcg gagaagcctc tcaatgagta ggttctcctc tgatattttc
     2221 tcgagcgcac gagtgtctca ccacactaca cgtggctgaa ttgctgacat gctgaagata
     2281 tagcgcctgc ggcgctatat cttcttgagg aatcgtgtta cagagaggaa gctgccgatt
     2341 acgccgatgc ctactcctac ggcgatcagg aggagcaggg tcgagaaggt gtttactgcg
     2401 tcgttggaga tcggtacgta ggggagggcg ttatgcgacc agtcgatgaa gagaaggttg
     2461 atccagatca cggtgaacgc cgcgaggcta gctccggcga aggcctgcag aaggccctcg
     2521 aagacgaatg gtgtgcgcac gaagccgtcc gaggcgccga ccagcttcat cacctcgatc
     2581 tccttgcgcc tggcgaagat ggagagcctg atggcgttgg agataagcag tacgctggcg
     2641 accagaaaca ggagcgtggc cccgtagagg ccccagatca cgtaggaggt tatggagttg
     2701 agacggtcta tggtctgctg ggggtagctc aggtttctct ccgtgaaccc ctgttccttt
     2761 agcctctcgg ctacagcgct ggcactatcc gggtcctcga gctgaatctg gatggaagga
     2821 gggaagacac ccaggtcaag gtccttgtag aggtcgggct ggtcctcgaa cttatccttg
     2881 aactcggcga acgcctgctc ttcggagatc tgggtcacga tcgcgacctc tggccagctt
     2941 tcgacttcgt tcgcgagggc gtcgacctcc tggcttgtgg cgttcggcat gaatgcctcg
     3001 acgctgacgt cttcgcgcac tgagcctatt atgctctgga tgtgggcgct gacgagcagg
     3061 ccgagcccga agaccaggat gcagatgaac gtggtggtga cggcggtgat gctcatgagc
     3121 aaattcaggc ggaggttctt gagggcctcg gccaaaaaga accccaggtt aaacctcatc
     3181 ggcgtacgcc ccctttacct tgtcgcggac gacacgcccc tcctccaatg cgactacccg
     3241 cttgcgcatc acgtccacca tctctctgtc gtgggtcgcg acaaggacag tggtgcctat
     3301 acggttgatc ctgtgcaata gctgcatgat gctgacgctg gttgtggggt cgaggttgcc
     3361 cgtaggctcg tcggcgacga ggatgggagg ctgagacacg aaggccctgg ctatggagac
     3421 gcgctgctgt tcgccgcctg agagcatatc tgggaacttg tgcatcttct cagagaggcc
     3481 cacgaggttc agtatctgcg gcaccttcgt gcggatcgta cgccgcctct ggcccgtgac
     3541 ctccatcgcg taggcgacgt tctcggcagc ggtcttgttc gggagcagct tgaagtcctg
     3601 aaagacgcac cctatatccc ggcgcagacg agggattctg cgccgcggca cggacgatag
     3661 cctggtgccg ttcacgtaga tggcccccgc cgagggttcc atctccttca ggatcaggcg
     3721 tactatggtc gacttgcccg agccgctagc ccctaccagg aacacgaact cacctgaatc
     3781 tatcccgagg ttcacccgct ccagggcgat ggtgtcgtta ccgtaaacct tggtgacgtt
     3841 gtcgaatcgg atcattactt ctccacgcat ggttcgaggt cccccctcca gggtcccggc
     3901 catcggagac gataacaaca accgcaggcg ttatcaacca ccggggtagg cgccgagagg
     3961 tatgctcggc acactaccgc ttagtatgat ccgcccttcc tcggcctcga cgccggtgat
     4021 ggtggtctgg taaggcagac ggtcgagggg gtactcgaag gacgtaccgg cgagcaaccg
     4081 gtctgcgagc tcatcgggca cctgaacccc cgccgccgtg aggccctgcg gctggaagat
     4141 caactctccg tcgcgcaagc caggctcccc ttcgatggag gccgggaacc gcctgccgag
     4201 caccgagacc tccgacctga cgaccacccc gtctttttgc acatccacgt cctgtattgg
     4261 tatctcagat cccgccttcg ccaagcgcga gacttcccac tcgggcacat cgacccgtac
     4321 atctcccgat aggggatccc tgtccaccac ctggcccttt ctcatggtgg cccagacgtc
     4381 tatgtcgaag gggtcgaggt cgatgcgcgc cctagttgcc gttacgttgc ccaggtcggt
     4441 gttcttcatt acgatgcggc cgcccgagaa ctcgcccgcc agcatgcgta ccggggggtc
     4501 gctcttcagc tcaacgtagg gctgcttttc gatgccgaga cgggccttga catctcgggc
     4561 caccgcgtac tccagcagcg acggcaagaa cgtgtagggt ccgatcagga ccacgacgag
     4621 tacgggaaga agcacgcccg ctacaacgct ccgcataacg ctaggccaca gtcctgttct
     4681 tcaggtaagc gtagacaaag gcatcgaggt cgccgtcgag gaccctgtcc acgttcgcag
     4741 tctcctcgcc ggtgcgatgg tcctttacga gcttgtaagg gtggagtacg tatgagcgga
     4801 tctgcgaacc ccactcgatg tcggccttct ccccgctctg cgcggcgatc tcctgctccc
     4861 gcttctcgcg ctcgagttcg aagaggcgcg ccctgagtac acgcatggcc acctcacggt
     4921 tctggtgctg gctgcgctcg ttctggcact gcgccacgat gccagtcggg aggtgcgtga
     4981 tcctgaccgc cgagtcggtc ttgttgacgt gctggccgcc ggcccccgag gctctgtagg
     5041 tatccacctt gaggtctttc tcctcgatct cgacctcgac ggtctcaccg ataacgggtg
     5101 cgacggctac ggaggcgaag ctggtgtgcc tccttgcccc ggcgtcgaaa gtgctgatcc
     5161 tgacgagacg atgtacgcca cgttcggcgg ataagagccc gaaggcatac tcccccgaga
     5221 cggtgaaggt ggcgctcttt atgcctgcct cctcgccttc tgtgtactcg atcacctcga
     5281 gcccgaagcc ccgccgctgg gcccagcgct ggtacatgcg cgccagcatc tcggcccagt
     5341 cctgcgaatc taccccgccc gcgcccgagt ttatggtcag tatggcgtca ccctcgtcgt
     5401 actcgccggc aaagagcctg gcgacctcct gctcctcgag tacccgctcg acccgcctga
     5461 gctcctccct gacctcgggg agcaggtcac cgtcctcttc ggcgagctcg aggagttcct
     5521 ccgaatccga gaggctctct ttgagcccgt cgagcagatc gactctgcct tttacccgcg
     5581 agaagcgggc cgataccgtt ctggcctctt ggggatcgtc ccagaaacca ggacggctca
     5641 tgtccttacc taactcttct gcctctgtgc gtaaccgatc cacatcgaag aacgcctcga
     5701 gctcagcgag gcgctcttcc agctcgtatg tcttgtcctt gagctctgtc actaacctgc
     5761 ccgggcttcg gacccgagcg gcggcgcgcc tggctgtccg tgacacttct tgtacttctt
     5821 gccgctgcca cacggacagg gctcattgcg cccgaccttg ctcgcggtct tgcgcaggct
     5881 cttcggacgc tggttcggct cttcgccgcc gctgtaggag agttgctgca tctcctcctc
     5941 gcgcagcttg atgttctcca cgcggtagat gtaggtgacg tagtccgcct tcaggccccg
     6001 ctccatctcc tggaagatgt cgaatccctc ccgcttgtat tcgaccagcg ggtcgcgctg
     6061 ggagagacca cgccagccga taccctcgcg caggtagtcc atctcgtaga gatgctcccg
     6121 ccagcgggaa tcgaccacag agagcagcgt ccggcgctcg gcctcctcga acgtggccac
     6181 cccgtcaccg cgggctagtc cgcgtttctc cagctcggcg gtccgctcgt cccactcggc
     6241 cttgcgctcc tcgaggcgct cgtcggcgtc ctcgaggacc atcgcgagga cctcggcggc
     6301 cgtaagggtc tcgacgtcga tagactcgaa gtcgatcctg ctaggataga agcggtttat
     6361 ctcggtcgtc agggcctcca ggtcccagtc ctcggggtag acgttctcgg agacgtactc
     6421 agagacgact tctgtgagca catcctccac gtaggacttc gtgtcgacgt cctcccccat
     6481 cagtatgtcg cgcctgatcg cgtagatcac ctctcgctgc ttgttgagga cgtcgtcgta
     6541 ctcgagtatg cgcttgcgaa tctggaagtt gcggctctcg acctgttcct gggcacgcct
     6601 cactgagttg gagatcatac ctgcctcgat cggcacgtcc tcttcgagcc cgatgcgctc
     6661 tatgatcccc tgcatccgct ggccgccgaa gagcctgagg agctcgtcct cgaaggacag
     6721 gtagaacctc gactcgccgg ggtccccctg cctccccgag cggcccctca gctggttgtc
     6781 tatgcggcgc gcctcgtggc gctcggtgcc gaggacatag agcccgccga gctcgggcac
     6841 tccctcgcca agcttgatat cggtaccgcg gcccgccatg ttcgtcgcta tggtcaccgc
     6901 gccccgctcg ccggcctcgg cgatgatctc cgcctcccgc tcgtgctgct tggcgttaag
     6961 gacgttgtgc tggataccac gccggcccag cagcgccgag agacgctccg agacgtctac
     7021 ggagaccgtg ccgacgagga tgggttggcc ctgtttgttt cgcgcgacga tgtcctcgac
     7081 gacggctttg tgcttggact tctcggtctt gtagaccagg tcgtctttgt cgtccctgat
     7141 catctccttg tgcgtcggga tggagacgac ttccatcttg taggtgtgca tgaactcatc
     7201 ggcctctgtg gcggccgtgc ccgtcatgcc cgagagcttg tcgtacatcc tgaagaagtt
     7261 ctggatggtg atggttgcta cggtctggtt ctcttccttg atctcgacgc cttctttggc
     7321 ctcgatggcc tggtggagcc cttcggagta acgcctgccc tcgagcacac gtcccgtgaa
     7381 ctcgtcgacg atgtagacct gcccgtcggt gacgatgtac tcatcgtcct tgtggaagag
     7441 cgtctgcgcc ctcagcgcct ggttgaggtg gttgacgagg ttggtgttga cgtcgtcgta
     7501 gaggttctct atgccgaggg ccttctcgac cttctctaca ccgctctcgg tcggggctac
     7561 ctgctgcttt ttctcgtcca cctcgtagtc ttcgccgctc ttcaggcgcg ggactatggc
     7621 ggcgaaacgg tagtagacgt cggcggcggt ctccggcatg cccgagatga tgagcggggt
     7681 gcgtgcctcg tcgaccagga tagagtcgac ctcgtccact atcccgaagt tgagctcgcg
     7741 ttgcacgagc tggtctggtg aagtcgctat gttgtccctg aggtagtcga agccaaactg
     7801 cgcgttcgtg ccgtaagtga catccgagtg gtatgcgatc ttccgctcgg cgaagtccat
     7861 gctctcctgg atgaggccga cctcgaggcc gagaaacgtg tatatctcac ccatccaagc
     7921 cgcgtcgcgg cgggcaaggt agtcgttgac ggtaacgacg tggacgcctt tgccttcaag
     7981 ggcattgagg taaacaggca tggtcgcagc gagcgtcttg ccctcgcccg tcttcatctc
     8041 cgcgatcttc ccctcatgca agactatgcc gcccatgatc tgctcgtcga agggacgcat
     8101 cccgagggta cgcctggaga cctcccgcac gacggcgaag gcctcgggca gcaggtcgtc
     8161 gatggtctcc cctccggcga gcctctcgcg aaactcgtcc gtcttctccc gcagtgccga
     8221 gtcggagagc ttctctatct cgggctccaa cgccgagacc gcctccacgc gctgctggag
     8281 gaccttgacc ttgcgaccct cgcccatcct caatatcttc gtcaacaagt tcgccaaaac
     8341 aagctacctc gtagtagtgg tcgtaccgga ctagcgggca ggctctatga gcccgtagtc
     8401 tccgtcacga cgccggtata ccacatttat atcaccggta tcggcgctcg tgaacacgta
     8461 gaagtcatgg tcgaggagct ccagctggag gacggcctcc tcagcgccca tcggcttcat
     8521 ctggaactgt ttggtcctga ctatgcgcgt ctcgatctcc tcttcttcct ctacgacgaa
     8581 gggtttcgcc tcgtcgggtg ccacgtccgc tgtattcttc agctggcctt gccagcggtc
     8641 gatctggcgc cccctgaagc gcttgacctg ccgctcgagc ttgtccgaca tcctgtcgat
     8701 ggaggcgtac atgtcttcgg aggcctcact ggctttcagc acagatccgt tgataaacaa
     8761 cgtggcctca gctacctcag gctcggagac ggcccggttc cgctcgtgga tgagctctac
     8821 ctcggcacgc gatgcggtgc gctcgtcgtc gaagaacctg gcgacccgct ccaccttctc
     8881 ccaggcgtag cgctccagag cctccgtcac cgggatgttg cggcccttga tgagtatgtg
     8941 catttcgctc ctcccttcgg tcgtcgcgag catttcagca cgcgccctgt cgggcccttt
     9001 cagcacttca gctttttggt gtcgattcac cgctggggtg cgaacgcgaa tcgttcgcca
     9061 cgatgaaaag cctctccata gaagcttccc tccgccatcc aagagctgcc tgaaggctga
     9121 agtgctgaca agcgaaggcg ttagcctgag cgtgctgaaa tgctaacatg tcctgcacag
     9181 actcagggcg tggacttctt ctgctcccgc ccggaccatt gtcccggcgc atgcgctcat
     9241 ggtcgctccc gtggtgaaga cgtcgtcgat cagcaagatc ttgccgcgca gaggttttgt
     9301 ggccgtgtaa gcgccggcga cgttggccct tctctgggcc gccgagagtt cgacctggtc
     9361 ccttgtgctg cgcacgactt ctagtgtatc cgatacggtt gctttcatct tc
//
LOCUS       DASTKM010000089        26208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1429759, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000089 DASTKM010000000
VERSION     DASTKM010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 26208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26208
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429759"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /gene="accB"
                     /locus_tag="VFQ10_03420"
     CDS             complement(<1..425)
                     /gene="accB"
                     /locus_tag="VFQ10_03420"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET9964422.1"
                     /translation="MDENRREPLPLREVEQLVELVRESGMGEIRVRQGEIEISVRARS
                     DVSATPGANQAASGTRGVSEVVEHTPEMDGLHAVRSPVVGTFYRAPAPGEAAYVEVGD
                     MVSTGQTLCIVEAMKLMNEIVADVSGEVVEVLAENAGGV"
     gene            501..2321
                     /gene="mutL"
                     /locus_tag="VFQ10_03425"
     CDS             501..2321
                     /gene="mutL"
                     /locus_tag="VFQ10_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET9964423.1"
                     /translation="METGRASINVLDPRVAQQVAAGEVVDRPASVVKELVENAIDAGA
                     SRIEVELGDGGTSRILVRDDGSGMSRDDAGLSVLRHATSKIRNVEDIEAVTTLGFRGE
                     ALPSIASVSSFSLTTSTGQGTATKVVVEGGEAAETSAASHPKGTTVLVDRLFYNVPAR
                     RAFLKGARAERSAIVEVVTHLAIAHPTLAFRLTEGGRDYLSLPEAGDLLERLAQILGV
                     AKARALRKVEYESGPFGVSGYAALPSLTEGTRAHQTISVNGRFVRAENLHRGLDDAYR
                     ATVPSGRYPPVAVEITADPRRVDVNVHPTKQVVRFSEERAAREAVAAALRDAIEWRTQ
                     APPQAPRAAERAFTQDRFSGSSRPPADYPSFAAEGRSPYTAPPARGLQEARERLSEAS
                     RPLAESLAEEREAYRDLPEKGGLPQASPEELRVIGQLAAGYILIEEPESLWVVDQHVA
                     HERAILDRLHDSDSPATVQSLLVPEVVDLSPAEAELALASLEELSVYGFEAEPFGPRS
                     VRVTAVISTLADRDIAGAFKDALSAISGTEPGHRREDHILATIACHSAVKMGDKLSQP
                     EMEALIKSWLESRLPATCPHGRSICYRIGLNEVARKLDRH"
     gene            complement(2331..2549)
                     /locus_tag="VFQ10_03430"
     CDS             complement(2331..2549)
                     /locus_tag="VFQ10_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964424.1"
                     /translation="MGRTSEVDEPGYVKGDRVCVLRGHGSREPGVVIGYYVDWGLYRV
                     VYMVDLAGRGPRAVEEWRLSFRREGEEC"
     gene            2689..3576
                     /locus_tag="VFQ10_03435"
     CDS             2689..3576
                     /locus_tag="VFQ10_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9964425.1"
                     /translation="MTRDRLLGAFAVVAAAFIWGTLGLFAKILYAQGVSFETLVAVRA
                     SIGWAAVLLFVLATGGARSLWVSGRDLVYLVPLGLVGIGFFYLLYFYTIRESTVGTAA
                     ILLYSSPAFVVVLARLFLKEALNAPKVLALFLTMGGIFLVAGGYDPANLEVSPKVLLA
                     GLLSGLTYGLYSIFGRPVAGRLRSSIILSYALFFGASLLVVAALPTLGTLAGLSVGSY
                     ALLLMLAVVHTALGFALYTFGLGRLGAGRAAIVATVEPVVAGALGAMLLGEDLTVPKV
                     VGAGLVISGAALAQGRVRK"
     gene            3687..4814
                     /locus_tag="VFQ10_03440"
     CDS             3687..4814
                     /locus_tag="VFQ10_03440"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET9964426.1"
                     /translation="MPIEFKEGGSDYSLGVEEELPIVDATTGELVQKIEEIMSRLPTE
                     LHESVSYELFQSVMEIKTPPCASTSEVEKHLRELRSRVGSWAAACGASLASAGTHPFS
                     RYRDQKVTDQERYKNVLRTLRWIAQREVIFGQHVHVAVPGPEEAIIAHNRLAEQTPIL
                     LALSANSPFWQGFDTGFESSRVKIFESFPRAGLPPAFPDYEAFEHYVDLMVESGAMED
                     YTFCWWDVRPHPKIGTIELRVLDSQTSLKNTLALTALTQCLVAQVLEEVGPHEPYNMH
                     LVVENKFRASRYGMDARFYDVREKITVPARDLARNLVERLQPHAQDLGCESELEGVLE
                     IVEGGTGSQRQREIYKESGNFLDVVAFLIEGTRPALAEEQS"
     gene            complement(4867..5148)
                     /locus_tag="VFQ10_03445"
     CDS             complement(4867..5148)
                     /locus_tag="VFQ10_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HET9964427.1"
                     /translation="MVTAIVLVRTERERVQEAAQAMIAIDGVTEAYSVTGPYDLVVMV
                     RIPDFESLADVVPERIAQVPGVARTETMVAFRAYSNYDLDRAWSLGFEE"
     gene            complement(5158..6249)
                     /locus_tag="VFQ10_03450"
     CDS             complement(5158..6249)
                     /locus_tag="VFQ10_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964428.1"
                     /translation="MGVRIERRSHRAAPDSHRPRPSGRPRRPHSRPKERRQRTLPAWL
                     TLLGSILLLVVVFGFIFFVARGCVATQQSTQIRKYVTSADSILSDSSNAGRGELQRIL
                     GNAGGNAARLDDEALAQAANRTEQIYLQALRQEEIPPEFEDDHHYVVTALGVRSQATD
                     ELVQAATGDASGFAGELAAAVEDYRTSDSIVANYYIPAVKHSLETAGQTSDQTYLEEP
                     QSFMDYGALGFDTAPVSGTARSDPNALHGVRVTAVRVAGKPLNSDGNVVLTGADEPTF
                     AVTVGNDGEVPETGVEVEVVLNTRAERQSESKTIARLEPKKMTVVEVGGFIPGELDET
                     AKVTVEAGPVEYEKRTANNILTGTVTFGI"
     gene            complement(6261..6344)
                     /locus_tag="VFQ10_03455"
     tRNA            complement(6261..6344)
                     /locus_tag="VFQ10_03455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6308..6310),aa:Leu,seq:cag)
     gene            6494..8122
                     /locus_tag="VFQ10_03460"
     CDS             6494..8122
                     /locus_tag="VFQ10_03460"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9964429.1"
                     /translation="MTETRYIEGQGRYALAEMVGRGGFATVWRARSLKGYLRGKDVAV
                     KIIPVYSAGERSRALREGQIAEGLRHKHIVETLEVIPGDHEIYLVTEYVRGMPLDEAA
                     KGYGVDEIVDSLAQILEALVYAHGQGIIHRDIKPQNALVDARGRVKLTDFGVAFRAGD
                     TRLTRVGFAVGTPGYIAPEILDGADPTALTDIYAVGATARTLLASQPYEPPPRLREFV
                     DRTTSPNPDHRPQSAWAAVKLLTGRKAPSGSISPDGRQVPDRLSGGFKDGALRIINGL
                     AAGCLGYLLAAEILTDGAQAAGVAAGLGVLAYLLPRLGALGVIVALAVLLIRNGAGLG
                     FSALVPVLGGVWVLGAGTANPDVRKLPLGPALAVPLALLVGLGTLLGTTLLAALPLLF
                     GALMRPLGACLSAGAGAFTLICYDLTVRNGFFADGPYAELPFSGAKLDVLPITLGVGG
                     LIDYSQGYLHYFNKLPLLILLWAAMAGVISLAEWAGKWVVGLAVAIVGGALGYALLLF
                     PKAPPGTLASDMISLGLAAIIYAVLRYLASRVRG"
     gene            8129..8956
                     /locus_tag="VFQ10_03465"
     CDS             8129..8956
                     /locus_tag="VFQ10_03465"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HET9964430.1"
                     /translation="MGFLKHIEKRMESLVEGVFGRAFRRQIHPVEIAKGLTKQMDEGR
                     MVSISRTYAPNDFTVHLSKEDTESIRAYQASLNDELIQYASTHAENKNYHLMTPPRIR
                     FEIEDTLRFGEFGVTAKLTGGDGPREKGAPQDTSGQTRIFRTEESSGGEIDQGTATIS
                     ADEARRHGLAREIVEVVLGDDKHQLEGRGPWSVGRSQENDIVIHDPNVSRRHARISRA
                     DNGFVVEDLGSTNGTLLDGAPIDRERIEGGDELTFGQSTARFIRRIDSPGEESGGRR"
     gene            8960..9445
                     /locus_tag="VFQ10_03470"
     CDS             8960..9445
                     /locus_tag="VFQ10_03470"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9964431.1"
                     /translation="MLELQVPLLAAKALILLLLFAFIYAVMSRSIGDLRKIPDDEPYV
                     PGRGHNGRGTYARRSSASGVPGLVVEDSEILAPDTRFELGDGETSMGRSSGSTIVLKS
                     DDYASGRHAQLTRHGGLLYVEDLGSTNGTFVNGRKTVGATPLRNGDTVRIGSTVFRYE
                     E"
     gene            9450..10658
                     /locus_tag="VFQ10_03475"
     CDS             9450..10658
                     /locus_tag="VFQ10_03475"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET9964432.1"
                     /translation="MPFLELQPFGVTDTGKVRQNNEDALLVGEGEDETLFVVADGIGG
                     FEAGEVASSLAVDVLKDLPPGESFKAAIGEANRRIVAAGRGDEKLSGMGTTVVAIRFG
                     GTHGEPVAEVAHVGDSRAYLMRGGDMKPITEDHSLVAELVRSGDLTRDQAAEHPQKNL
                     ITRALGADEEVDVDTAVLSVEAGDRVLLCSDGLSDMVSEAGISEILAQSPDDPERAAR
                     GLLSAALDAGGNDNITVVVVDVMEQPAREADARNERERHSSGISELLAAKPSVKDTGA
                     RTPSRGKAAPSRPRRPTRAARRKRPSRLRKALGKLVRGLAIILVLVAALTPVYLWGSS
                     RYFFEFDEGEVVAYQGLPYAPFGIELNQEWRRPGLTESQIKEPYQKPIENHKLYTRDQ
                     AERVIEDLGS"
     gene            10660..11973
                     /locus_tag="VFQ10_03480"
     CDS             10660..11973
                     /locus_tag="VFQ10_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET9964433.1"
                     /translation="MTQRAFTPMILALLVTTLGFATLIFVQEAASPPLIYGAYYAGTL
                     FVLYLGVRLLLPHSDVLLLPIVTLLTGLGLVMIFRLTYNVEGAKQLALTQAVWILMGS
                     GAMFLLVLFFRNYDRIFDYKYLLAIAAVFLIASTFTPLGYSVNGARLWVDLGPVNFQP
                     SEFARIALVIFFAGYLAEKRDVMAATSRSFLGIQLPAIKYFGPVALVWVASFGLLVFE
                     KDLGSSLLFFAVPLLMLYAATGRTAYVLLGGVLFTLGSFVTFLLFEHVQLRVQSWLDP
                     WRDPDGTGFQILQSIFNIADGGLLGTGLGAGFAQTIPEVETDFVFSAIASELGLLGAT
                     AVLLAFLAFIYRGTKIALLAEDDASKLLAFGLTAMFALQTLIIVGGVTKAIPLTGITL
                     PFVSYGGSSVVGSFILTGLLLLVSERAGRREAEGLAYRDDPWGAP"
     gene            11958..13406
                     /locus_tag="VFQ10_03485"
     CDS             11958..13406
                     /locus_tag="VFQ10_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9964434.1"
                     /translation="MGGSMNAHLRRTFYLFVAGFVGLVVMLVYWQVYSREALANNPEN
                     GFQTQRSVSSPRGLILAGDGETVLAKSVKRGTPTGPVYDRVYPLGEPFANVVGYWSTR
                     YGATGIEISNNSDLSSVAGEPETLDDLINRATGGPQPGNNVVLTLDPELQELAYQELS
                     ASSTGRGAVVALNPKTGEILAMATSPSFDPNDVDENYPDLASAPNAPLVDRAAQSLYP
                     PGSVFKVITAAAALEQGVSPQQTFFDSGAYELPGYTVRNFEGKDYGEVTFTRALAYSI
                     NVVFAKIAVEIVGAEALTQMAVNFGFGDPYDDFPLPVSGSSVNSLPPEQWTTGTLAQT
                     GFGQGEVQTNAFEMALVAGAVANDGEIMEPRIVREVRSPDGIILDKPTPSVRRQALPP
                     GTANTLNGMMQEVIIQGGFTEAEIPGMKVAGKTGTAESGNGLPHSWWVTFAPADDPEI
                     AMCVMIENGGRADRSAMPLAARLMKAYLEGGV"
     gene            13441..15534
                     /gene="pknB"
                     /locus_tag="VFQ10_03490"
     CDS             13441..15534
                     /gene="pknB"
                     /locus_tag="VFQ10_03490"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET9964435.1"
                     /translation="MQQLVDNRYRLVKSLGSGGMADVYLAHDDILDRDVALKVMSNRY
                     ASDEEFVERFKREAQSAAALSHPNIVSIFDRGESEDGTYYIAMEYLPGGTLKNRIVKR
                     GALPARTAAAVALQMAEALRAAHERDVIHRDIKPHNILITGLGDVKVTDFGIARAAAS
                     STMTRTGHILGTAHYISPEQAMGEPVGPASDLYSLGVVLYEMLTGELPFDAETPLGIA
                     MKHVNGHLRQPMELNPAVPDGINAITLRLLAKNPEGRYSSDAELIEDLERVCAGLDPS
                     GTTTEMMPHAVAATTRLNPPSPPQRIGRNKVRRRRAAPPLLALLALLVLAALTWGGYI
                     LLQDRQAAQIGVPDLVGMSLDGAREKVGDDFEVEVAKSVSGERPVDTILGQSPSTGKA
                     EKGSTISVNVVGTRVSDVPDVVGSGRAEAEQALKAAGFQVEVDERESSFDDQNRVMGQ
                     DPSGGTRAEVGSGVTITVGTGPSSVPIPRLYGKTPAQAKSVLLGAGLRLGTQNRDYNS
                     DVAEGVIYFQNPSGGENVEPGTAVDVTVSLGPEQVEVPEIYGLSVAAAQTALSNAGLG
                     STPIEVEGDEPAGTALATDPGVGAMLDPGSTVSLYYSAGPPPATTASPDASQSPNSEP
                     SQGPDQGTQDEGNTDAKPAADKQPADRPQRNERTQDNSGPDSSGQDSSGQGSSGSGNG
                     VGLGTNEGQGSGSEG"
     gene            complement(15538..15801)
                     /locus_tag="VFQ10_03495"
     CDS             complement(15538..15801)
                     /locus_tag="VFQ10_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964436.1"
                     /translation="MEGGQAQGSVRIRATSPGEYPILVVELPSGGLRAVYFETGYDLG
                     RAKTVEEDWLLENAVGRHSFVELDPPVETPAGYLGDYVRRELL"
     gene            15882..18380
                     /locus_tag="VFQ10_03500"
     CDS             15882..18380
                     /locus_tag="VFQ10_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9964437.1"
                     /translation="MTFIFVEGVTMSDPIPEDGGTEAGASEGIADEPLLDRLPPEDLP
                     EELLLRRLGRLDTRRALAEICRNVPALEEILLPRGIQADALTRKSGGGAPLLRGLARR
                     ISHDAAAWGAFRTAIQEQIPPETFEALEGFSPENLEELKQAHTTEGLLLAALSAEEEP
                     EEGVVETLLSAWREERGQAEKRASEDARVRDLEAELERLKQDNERLSFGARAAKERAA
                     ALAEEVEVLTGERTDTSELVRKAEERAASALDLRGQLDERIETLERRSAQLEKALEGE
                     RSAYSRAAERVEELHEELGRVVEERDRIKNALQDARFTDRGFGELLVRAIKNEVAALP
                     ASLDSTAQAARLMEFMGKVLQAHSDLRSPGEQGRRTSEEDPDDKDPSPASRRRKTDGP
                     ARSAALPRVGGYARPRPTLSFRALGGAGEVGGSSHLLDFGKTRILVDAGIKPDGRGPV
                     TPDFGGLDGLDAAVVTHAHLDHCGALPLLVRDRPKMPIYCTPPSAKLIISALNDHAAM
                     GGGLPGGAPIHEVRKRLVPVPFGKPLDVGGAKVTLTESGHILGAASVLFSSGEATVFH
                     TGDICMEDHFSIPSARLPEVRDIDLLIMEATLADQKPQPFSESIRTMVEVINDTTLDR
                     GGTVLIPTYALGQAQEIILGIKHYGREYGLDREVFIYVDGSVVTTSERLYAEQLGYMK
                     PYLQHGDPREIFFSENIRAVANDDKARERILSNPCAIIASPVTMQGGASAFYRKRLEK
                     SSENAVILPSNAASSYGTHRAEGEEEQWRVERVSFAAHCTQDELLGITEKLSPRQIIL
                     IHGSRRRISDLAFRLAPNHKIHTPTVGETVRTVL"
     gene            complement(18516..19142)
                     /locus_tag="VFQ10_03505"
     CDS             complement(18516..19142)
                     /locus_tag="VFQ10_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964438.1"
                     /translation="MGFEESARARLRRYREEADVSLELVREVETMARRFFGRISAGLE
                     YTAVLGRQAGFEVETAYAGGALDVRAVAGPGAEAGVSFGLVSGAAAETDEDLMHEELS
                     RYILEPSGYSGRILGWSTDAGDAPCQIFAVYRDGVWKTRGRFVARARGRVDDPDDVLN
                     GFCLRILGRLIDLASLSGGAGRSWTDEPYALRDLLEGRRAPTALRWPR"
     gene            complement(19147..19929)
                     /locus_tag="VFQ10_03510"
     CDS             complement(19147..19929)
                     /locus_tag="VFQ10_03510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9964439.1"
                     /translation="MARSLKGKVAVVTGASSGIGEATARELASRGASVVLASRAVDKL
                     EALQRSISASGGVAVAVETDVSERGSVEAMVGRAVGELGSLDVLVNNAGLGLSGRITE
                     VRTEDVRHVFEVNAIGPLNCIQSALVYMGKGGRIINVSSVVGRRAIPKVGAYCASKFA
                     LNALSDALRVEVADRGISVTSVYPGTTSTSFRENSRRTRDEKRGWRPKGVRPERVAQK
                     IADAAERGPRDVYVTIPDRLFVAGVTLFPGLADRVLRAWAKD"
     gene            complement(19978..20154)
                     /locus_tag="VFQ10_03515"
     CDS             complement(19978..20154)
                     /locus_tag="VFQ10_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964440.1"
                     /translation="MVSKMQSLRWLENLKRSQRLTAREERRKEEVMEIFRELYDISLR
                     HQDPSIFPPWRPSW"
     gene            complement(20512..21114)
                     /locus_tag="VFQ10_03520"
     CDS             complement(20512..21114)
                     /locus_tag="VFQ10_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9964441.1"
                     /translation="MGGERERLGLVWIDCSYPVVAAGLKEALEGRARVHVGDTAPTDA
                     EVPYCAVFDTSGAEGISEGIERIRGVNADIPILVFSLHVDLPLARAALRHGARGFIHA
                     GMELDQIARAVEVAHEGEIVAPRKLLEYLIANDDPTGLDALTIRQREILQLVAQGLSN
                     AQIAKNLFLSESTVKQHMRATYMVLGVSDRKEAARLINGR"
     gene            21331..21873
                     /locus_tag="VFQ10_03525"
     CDS             21331..21873
                     /locus_tag="VFQ10_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HET9964442.1"
                     /translation="MASVLVGITGGIAAYKAPGVIRRLREAGNEVRVVATDAAFRFVP
                     EETLSIAAGAGVHTDVTWWEHSGRVEHVSLARWADLVLIAPATADSMARAAIGLGDDL
                     LSATILAGAKRVLWAPAMNPEMWQSPATKRNVDTLKSWGHRFVGPAEGLMASAEEEPG
                     VGRLAEETEIVAAVREILAT"
     gene            complement(21949..22464)
                     /locus_tag="VFQ10_03530"
     CDS             complement(21949..22464)
                     /locus_tag="VFQ10_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9964443.1"
                     /translation="MEVEYLISEYKFCPRCGNRLRVRLLKRGEPERLVCESCGFVFYL
                     GPKLVAGAIIEIDGRIVLIQRDIEPGYGKWTFPGGFVERGERVEAAAEREVLEESGLE
                     IEVGEIVGLYTYDGQIPAIAVFTAEVTGGEPTPLDETMDIRCFPREGLPWTEMAFPST
                     EQALKDYLRRI"
     gene            22517..22589
                     /locus_tag="VFQ10_03535"
     tRNA            22517..22589
                     /locus_tag="VFQ10_03535"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22550..22552,aa:Gly,seq:ccc)
     gene            complement(22788..23600)
                     /locus_tag="VFQ10_03540"
     CDS             complement(22788..23600)
                     /locus_tag="VFQ10_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET9964444.1"
                     /translation="MSEVNGNNPGGVTSKAHSPVPNEELGTSDKMAKLRIFRGDAEGG
                     EEVEYEIPLVEGMVVLDAVLAIQAQQAPDLAVRWNCKAAHCGSCSAEINGRPKLLCKT
                     RIEEYDGGEIYVGPMRAFPVIKDLVSDVSWNYEVSKGIKPFQTSEQAPFTMYEEDVER
                     LYEPKKCIECFMCQDVCHVLRTHHKHPEFAGPRFFVRNQWLEMHPMDEADRLADLKED
                     NGLGYCNITKCCTEVCPVDIHITDNSIIPLKERVADEYYDPAKWLMRKIRRS"
     gene            complement(23597..25363)
                     /locus_tag="VFQ10_03545"
     CDS             complement(23597..25363)
                     /locus_tag="VFQ10_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9964445.1"
                     /translation="MELQRDGQNGHREAYETREHDVLIIGAGGAGLRAAVAAAEHGLS
                     VGIVTKSLLGKAHTVMAEGGIAASVGNVDPDDSWRQHFIDTMRSGKFLNNWKMVETFT
                     KEAPDRVYELEQWGALFNRTPEGKISQRPFGGHTYRRLCHVGDRTGLELIRTMQEKVL
                     ATDAKVYMETTVTKLFKKEGRVVGALAYTRENGKFVHFKARAVVMATGGWGRIFKVTS
                     NSWEGTGDGVVLGYDAGAELVDMEMMQFHPTGMVWPPGVRGLLVTEGVRGEGGLLRNS
                     EGVRYMENYDPEKMELSTRDVVARANYTEVQEGRGSEHGGVYLDITHLGYDGIMKKLP
                     TMYEQFLKLADIDISREPMEVFPTVHYTMGGIKIDPESAASSVPGLFAAGEVAGGLHG
                     ANRLGGNSLSDLLVFGRRAGEGAAAYIEESVHSAEIDEGEVLHEIGRVLEPLEKKEEG
                     ESPYLIQQELQETMMEHANLMRDGDSLEEGLGKILALKDRLPQISVPGSRLFNPGWHT
                     AQDVRYLVQISEIIIRTALERKERRGAQWRLDFDGPDEELGKINFIVKKDGQGEVGIE
                     RREIPPMPEHLASLVEEAKAKA"
     gene            complement(25367..26113)
                     /locus_tag="VFQ10_03550"
     CDS             complement(25367..26113)
                     /locus_tag="VFQ10_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964446.1"
                     /translation="MSSIAAAWKRLPWWVEPVSVVIVLSVFGLYSFLIVSFLPGEYKD
                     YLSPFYSPPIPHPDWVPDFITAPMFIIWMPLGFRATCYYYRKAYYRAFFWDPPACQAG
                     AQEREPRKAENYRGERAVFVLNNVHRYFLYASFVVLAFLWYDTVLTFFPGGSFGVKVG
                     SIIWLVNVILLSTYSLSCHSLRHLVGGNKDCYTCVRGGNARRKVYNGISRLNLRHPQW
                     AWYSLFSVLLTDVYFRLIQAGVIPDLTILG"
BASE COUNT         4754 a         8597 c         8178 g         4679 t
ORIGIN      
        1 tcgacgccac cggcgttctc ggccagtacc tcgacgacct cccccgagac gtcggcgacg
       61 atctcgttca tcagcttcat cgcctcgacg atgcaaaggg tctgcccggt gctgaccata
      121 tcccccacct cgacataggc ggcctcaccg ggagccggcg ccctgtagaa ggtcccgacc
      181 acgggcgagc gcacggcgtg caaaccgtcc atctcgggcg tgtgctccac cacctcagag
      241 acaccccggg tccctgatgc agcctggttg gccccaggcg tggccgaaac atcggatctg
      301 gccctgaccg agatctcgat ctccccctgg cggacccgta tctcccccat cccgctctcg
      361 cgcacgagct cgacgagctg ctcgacctcc cttagcggca gcggttctcg ccgattctcg
      421 tccatctctg gcatagtcag ggattctaca cgccagcccg tgggaaaacc cgcccccgga
      481 tgtacaattg cccaagatcc atggagaccg gacgcgcgag tatcaacgtc ctcgacccca
      541 gggtggccca gcaagtggcc gccggggagg tcgtggaccg cccggcttcg gtggtcaagg
      601 agctcgtcga gaacgccatc gacgcaggcg cctcccgcat cgaggtcgag ctgggagacg
      661 gcggcacctc gcgcatcctg gtccgcgacg acggctcggg gatgtccagg gacgatgcag
      721 gcctctccgt actcaggcac gcgacgagca agatccggaa cgtcgaagat atcgaggccg
      781 tcaccaccct ggggttcagg ggcgaggcac tcccctctat cgccagcgtc tcctctttct
      841 cactgactac ctccacgggc cagggcacgg ccaccaaggt cgtcgtcgag ggtggggagg
      901 cggccgagac ctcggccgcc tcccacccta aggggacgac agtcctcgtc gaccggcttt
      961 tctacaacgt ccccgcaaga cgcgcctttc tgaagggcgc gcgggcggag cgctcggcga
     1021 tagtggaggt cgtgacgcac ctggcgatag cccatcccac gctcgccttc cgcctgacgg
     1081 agggaggcag agactacctc tcgctgcctg aggctggaga tctactcgag cgcctggccc
     1141 agatcctggg cgtcgccaaa gcgcgcgcgc tgcgcaaggt ggagtacgag tcgggacctt
     1201 tcggcgtctc aggctacgca gcccttccaa gcctgacgga gggcacaagg gcccaccaga
     1261 cgatctcggt gaacggccgc ttcgtcaggg cagagaacct ccaccgcggc ctcgacgacg
     1321 cctacagggc gaccgtgccg tccgggcgct accccccggt cgccgtcgag ataaccgccg
     1381 acccccgccg cgtcgacgtg aacgtccatc ccacgaagca ggtcgtccgc ttctcggagg
     1441 agagggcggc ccgcgaagcc gtggccgccg ccctcagaga cgccatcgag tggcgcaccc
     1501 aggcaccacc acaggcccca cgtgcggccg aaagagcttt cacccaggat cgcttttcag
     1561 ggtcctcgcg tccaccggca gattaccctt ccttcgccgc tgaagggcgc tccccttaca
     1621 cagcgcctcc cgcgcgtggc ctgcaggagg cgcgcgaaag gctctccgag gcttccaggc
     1681 cgctcgctga gtcgctcgcg gaagagcgcg aagcgtacag ggacctgccg gaaaagggcg
     1741 ggctgccaca agcctccccc gaggaactgc gggtcattgg gcaactcgcc gccggctaca
     1801 tcctcatcga ggaaccggaa tccctctggg tcgtcgacca acacgtcgcc cacgagcggg
     1861 ccattctgga caggcttcac gactcggaca gcccggcgac ggtgcagagc cttctcgtcc
     1921 ccgaggtcgt cgacctctca cccgctgagg ccgagctggc cttggcaagc ctcgaagagc
     1981 tctccgtcta cggcttcgag gccgagccgt tcgggccgag atccgtgagg gttacggccg
     2041 tgatctccac gctcgccgac agggatatag ccggcgcatt caaggacgct ctctcggcca
     2101 tctcgggcac cgagccgggc caccgccgcg aagatcatat cctcgccacc atagcctgcc
     2161 actctgccgt aaagatggga gacaaactct cccagcccga gatggaagca ctgataaaga
     2221 gctggctaga aagccgcctc cctgccacct gcccacacgg gaggtctatc tgctatcgga
     2281 tcggcctgaa cgaggtggct cgcaaactcg accggcacta aagcagcatg tcagcactcc
     2341 tctccttctc ggcggaagga gagtcgccac tcctctaccg ctctggggcc tcgcccggcg
     2401 aggtcgacca tgtatacgac cctgtaaagt ccccagtcga cgtagtagcc gatcaccacg
     2461 ccgggttcgc gcgagccgtg tcccctcagg acacacaccc gatcgccctt cacgtatcct
     2521 ggttcgtcta cctcggaggt tctgcccata ggccgctctc tcccgactac caagcttctc
     2581 cgaaaagggt actacgtgga gcatccggag atcgtcccct cggcgcgacg gtggagctta
     2641 ccgtataaat accgcttgcg ggcaccacag acgggagagc tcctcgacgt gaccagagac
     2701 agattgctcg gcgcattcgc cgtcgttgcg gcggccttta tctggggaac gctcgggctg
     2761 ttcgccaaga tcctctacgc ccagggtgtc tccttcgaga cgctggtcgc ggtgcgggct
     2821 tcgatcggat gggcggccgt actgcttttc gtcctggcaa ccggcggcgc caggagcctg
     2881 tgggtttcgg ggagggacct ggtgtacctt gtccctcttg ggctcgtggg gataggcttc
     2941 ttctacctgc tctacttcta tacgatcagg gagagtacgg ttgggacggc ggcgatcctg
     3001 ctctacagct cgcccgcatt cgtggtggtg cttgcgcggc tgttcctgaa ggaggcgctc
     3061 aatgcgccga aagtactcgc gctcttcctg accatgggcg ggatcttcct ggtcgctggc
     3121 ggctacgacc cggcaaacct ggaggtgagc ccgaaggtgc tgctggcagg actactctcg
     3181 ggcctgacct acgggttgta ttccatcttc ggccgtcccg tggcaggccg ccttcgttct
     3241 tccatcatcc tgagctacgc actcttcttc ggagcctccc tactcgtcgt ggccgcgctt
     3301 cccacgctcg gtacgctggc cggcctctcg gtgggctcct acgccctgct actgatgctc
     3361 gcggtggtgc ataccgcgct ggggttcgcg ctctacacct tcgggctcgg gcgcctcggg
     3421 gcggggaggg cggctatagt ggccacggtc gaacccgtcg tggcaggcgc cctcggggcg
     3481 atgctgcttg gggaggacct taccgtaccc aaagtggtgg gcgcagggct cgtgatctcc
     3541 ggcgccgcac tagcccaggg cagagttcga aagtagtcgt tcttggcctt tgttcgccaa
     3601 agcgggtact atgttgcccc acgttgagaa ctgccgcctg caagagatcc acttccctcg
     3661 aacacggagg ctagaggagg gatgccatgc ctatagagtt caaggaaggc ggttcggact
     3721 actccctcgg ggtcgaggag gagctgccca tcgtagacgc gaccaccggt gagctggttc
     3781 agaagatcga ggagatcatg agccggctgc ctactgagtt gcacgagtcc gtctcctacg
     3841 agctgttcca gtcggtgatg gagataaaga ctcccccctg cgcctcgacc tcagaggtcg
     3901 agaagcacct gcgcgagcta cgcagccggg tcggctcgtg ggccgcggcc tgcggcgcct
     3961 cgctcgcctc tgcggggacc catccctttt cccgctacag agaccagaag gtcacggacc
     4021 aggagcgcta caagaacgtc ttgaggacgc tgcgctggat cgcccagcgc gaggtgattt
     4081 tcggccagca cgtccacgtg gccgtccctg gcccggagga ggcgatcatc gcccacaacc
     4141 gactcgccga gcagacgccg attctcctgg ctctctcggc caactctcct ttctggcaag
     4201 gtttcgacac aggcttcgag tcttcaagag tgaagatctt cgagagcttc ccgcgtgccg
     4261 gcctccctcc cgccttcccc gactacgagg cattcgagca ttacgtggac ctgatggtcg
     4321 agtccggcgc gatggaagac tacaccttct gttggtggga cgtgcgcccg caccccaaga
     4381 tcgggacgat agagttgagg gttctcgact cccagaccag cctgaagaat accctggccc
     4441 ttaccgccct gacccagtgc ctcgtcgccc aggtactcga ggaagtcggc ccgcatgaac
     4501 cctacaacat gcacctggtg gtagagaaca agtttcgcgc ctccaggtac ggtatggatg
     4561 ctaggttcta cgacgtacgc gagaagatca cagtcccggc cagggatctc gcccgtaacc
     4621 tcgtcgagcg actgcagccc cacgcccagg acctaggttg cgagagcgag ctggaaggcg
     4681 tcctggagat agtggaaggc ggcacgggta gtcagcgcca gcgcgagatc tacaaagaga
     4741 gcggtaactt cctcgacgtc gtcgccttcc tcatagaagg tacccgcccc gccctcgccg
     4801 aagaacaaag ttaggcttca ggcaacaggt atcagcagat cttcctgaag tctgttaccc
     4861 gtagcctcat tcttcgaagc caaggctcca ggcgcggtcc aggtcgtagt tcgagtaagc
     4921 gcggaaggcg accatggtct cggtgcgggc gacgcccggg acctgggcta ttctttcggg
     4981 gacgacatcg gcgaggctct cgaagtcggg gatacggacc atgacgacga ggtcgtaggg
     5041 gcctgtgacc gagtaagcct cggtgacgcc atctattgcg atcatggcct gggcggcttc
     5101 ctggacgcgc tcccgctccg tcctgacgag gactatagca gttaccatgc agactcctta
     5161 gatcccaaac gtcaccgtcc ccgtgaggat gttatttgcc gtacgcttct cgtactcgac
     5221 aggccctgcc tcgacggtaa ccttggcggt ctcgtcgagc tcgccgggta taaagccgcc
     5281 gacttccacc accgtcatct tcttgggttc gaggcgcgct atggtcttgc tctcggactg
     5341 gcgctcggct ctcgtgttga ggacgacctc gacctcgact cctgtctcgg gcacctcacc
     5401 gtcgttcccg acggtaactg cgaaggtcgg ctcgtccgcc cctgtcagga caacgtttcc
     5461 gtccgagttc agtggcttcc ctgcgaccct gacagccgtt acccgcaccc cgtgcagggc
     5521 gttcgggtcg ctccttgcag tgccagatac gggggccgtg tcgaaaccga gcgccccgta
     5581 atccataaag gattgtggtt cctcgaggta ggtctggtcg cttgtctggc ccgcagtctc
     5641 cagggaatgc ttgacggccg ggatgtagta gttcgcgacg atgctgtcgc tcgtcctgta
     5701 gtcctcgacg gccgccgcga gctccccggc aaacccgctc gcatcccccg tagccgcctg
     5761 cacgagctcg tctgtggcct gggaccttac gcccagcgct gtaacgacgt agtgatggtc
     5821 gtcctcgaac tctggaggta tctcctcctg cctcaatgcc tgtaagtata tctgttccgt
     5881 acggttggcc gcttgggcga gcgcctcgtc gtccagcctg gcagcgttcc ctccggcgtt
     5941 gcccaggatg cgctggagct cccctctgcc cgcgttggag gagtcgctca agatgctgtc
     6001 cgcgctcgtg acgtacttgc ggatctgggt cgactgctgg gttgccacgc agccgcgggc
     6061 tacgaagaag ataaacccga agaccacgac taggaggagg atggagccga ggagggtgag
     6121 ccaggcaggg agcgtcctct ggcggcgctc tttcggacgg ctgtggggtc tcctgggacg
     6181 acccgacggc ctcggacggt gagagtcggg ggcggccctg tgagatctac gctctatacg
     6241 aacccccaaa gaacatgtcg tgggcgaggg gggactcgaa cccccacggc ctctcggcca
     6301 ctagaccctg aacctagcgc gtctaccaat tccgccactc gcccgcaggc cctctccggg
     6361 agacgtcctg aactgcgggg gattgtacca gcatatcagc acgccgaccg aagggaggcg
     6421 gcgggctgac aagcggaggc gaagcctaag cgtgctgata cttgataggc tatacaatag
     6481 tctatcaagt atcgtgacag agacgcgcta catcgaagga cagggacgct acgcgcttgc
     6541 cgagatggtc gggcgcggcg gcttcgcgac cgtgtggcgc gcccgttctc tgaagggcta
     6601 cctcaggggc aaagacgtgg ccgtcaagat catccccgtt tacagtgctg gcgagcgctc
     6661 gcgcgccctg cgggagggcc agatcgccga agggctcagg cacaagcaca tagtggagac
     6721 cctcgaggtc atccccggcg accatgagat ctacctcgta acggagtatg tgcgggggat
     6781 gccgctcgac gaggcggcca aagggtacgg cgtagacgag atcgtggact cgttggccca
     6841 gatcctcgaa gccctggtct acgcccacgg gcagggcata atccaccgcg acataaagcc
     6901 gcagaacgcg ctcgtggacg cccgcgggcg ggtaaagctt acggacttcg gggtcgcgtt
     6961 cagggcgggg gacacacgcc ttacgcgcgt cggcttcgcc gtgggcacgc ccggctacat
     7021 cgcgcctgag atcctggacg gcgccgaccc gaccgcgctc acggacatct acgccgtcgg
     7081 cgccacggcc cgtaccctgc tcgcgagcca gccctacgag ccacccccgc gcctcaggga
     7141 gttcgtcgac cggacaacct cgcccaaccc ggaccaccgc ccgcagagcg cgtgggccgc
     7201 cgtcaagctc cttactggca ggaaggcccc atcgggcagc atctcgcccg atggacgcca
     7261 ggtgcctgac aggctctcgg gtggcttcaa ggacggtgcg ttacgcatca tcaacggcct
     7321 ggccgcaggg tgtctagggt atctcctcgc ggccgagatc cttacggacg gcgcgcaggc
     7381 ggccggcgtc gccgcgggac tcggcgtgct tgcctacctg ctgccgcgcc tcggcgccct
     7441 cggggtcata gtcgccctcg ccgtcttgct gatacggaac ggggctggcc tcgggttctc
     7501 cgcgctcgtg cccgtcctcg gtggcgtctg ggtgctgggc gccggtacgg ccaaccccga
     7561 cgtgagaaag ctgccgctag gacctgcgct agccgtgccg ctggccctcc tcgtcgggct
     7621 gggaacactc ctggggacca ccctccttgc cgccctgccg ctgcttttcg gcgcgctgat
     7681 gcggcccctc ggcgcgtgcc tttcggctgg ggccggggcc tttaccttga tctgctacga
     7741 cctcacggtg cgcaacggct tcttcgccga cggcccttac gccgagctgc ccttctcggg
     7801 ggcgaagctc gacgtcttgc cgatcacgct gggagtcggt ggcctgatcg attattccca
     7861 ggggtacctg cactatttca acaaattgcc gctcttgatc ttgctgtggg cggcgatggc
     7921 aggtgtcatc tcgctcgccg agtgggcagg caagtgggtc gtaggcctgg cggtcgcgat
     7981 agtcggggga gccctggggt acgcgctact ccttttcccg aaggcccctc ccggcacgct
     8041 cgcttcggat atgatatcgc tcggtctcgc ggctataata tacgcggtgc tcaggtatct
     8101 cgcatcgagg gtgagagggt aggtttcttt gggttttctc aaacacatcg aaaagcggat
     8161 ggaatcgttg gtagagggcg tctttggaag ggcgttcagg cggcagatcc accccgtaga
     8221 gatcgccaag ggccttacca agcagatgga tgaaggcagg atggtttcga tctcgcgcac
     8281 ctacgccccg aatgatttca cggtgcacct ctccaaagag gatacggagt ccatcagggc
     8341 ctaccaggca tctttgaacg acgagctgat ccagtacgct tcgacccacg cggagaacaa
     8401 gaattaccat cttatgactc ccccaaggat acgcttcgag atcgaagaca cactccgctt
     8461 tggggagttc ggcgtcaccg ccaagcttac aggcggcgac ggtccccgcg agaagggcgc
     8521 cccgcaggac acctcgggcc agacccgcat cttccgcacc gaggagtcga gtggcgggga
     8581 gatagaccag ggcaccgcca cgatatccgc cgacgaggct aggcgccacg gtctggcgcg
     8641 ggagatcgtg gaggtcgttc tcggtgatga taagcaccag ttggaagggc gcggcccgtg
     8701 gagcgtaggt cgctcgcagg agaacgacat cgtaatacac gacccgaacg tgtcgagaag
     8761 acacgccagg atctcacgcg ccgacaacgg ctttgtcgtg gaggacctcg gttctacgaa
     8821 cggcaccctg ctggacggcg cccccataga ccgcgaaagg atcgaaggcg gcgacgagtt
     8881 gaccttcggc cagagtacgg cccgattcat ccgccgcata gactctccgg gcgaggagtc
     8941 cggcgggaga cgttagggga tgctcgagct ccaggtgccg ctgctcgcgg cgaaggctct
     9001 gatcttgctc ctgctcttcg cctttatcta cgccgtaatg agccggagca tcggggacct
     9061 gcggaagata ccggacgatg agccctacgt gccgggccgg ggtcacaacg ggcgcgggac
     9121 gtacgcccgc cggagctccg cctcgggggt tcctgggctg gtggtcgagg attcggagat
     9181 cctcgccccc gacacgcggt tcgagctcgg ggacggcgag accagcatgg ggcgctcgtc
     9241 tggcagcact atcgtcctga agagcgacga ctacgcctcg gggcgccacg cccagctcac
     9301 caggcacggg ggattgctct acgtcgagga ccttggctcg acgaacggga cattcgtgaa
     9361 cgggcgcaag accgtgggag ccacgccctt gaggaacggg gacaccgtca gaatcggctc
     9421 gaccgtcttc aggtacgagg agtaggatcg tgcctttcct cgagttgcag cctttcggcg
     9481 tcaccgacac cggcaaggtc cgccagaaca acgaggacgc cctgctcgtc ggcgaaggcg
     9541 aggacgagac gctcttcgtc gtcgccgacg gcataggcgg cttcgaagcc ggggaggtcg
     9601 cgagttccct cgccgtagac gtgctcaaag atctcccgcc aggcgaatcc ttcaaagcag
     9661 ccataggaga ggcgaaccgc aggatagtgg ccgctggccg cggtgacgag aagctctcgg
     9721 ggatgggtac taccgtggtc gccatcaggt tcggtgggac gcatggagag cccgtggccg
     9781 aggtggcgca cgtcggtgat tcgagggcct acctcatgcg tggtggcgac atgaagccga
     9841 taacagagga ccactcgctc gtcgccgagc tggtgcgtag cggcgacctg accagggatc
     9901 aggccgcaga gcacccccag aagaacctca tcacccgtgc ccttggcgcg gacgaggagg
     9961 tcgatgtcga caccgccgta ctctctgtag aggccggcga ccgcgtcctg ctctgttctg
    10021 acgggctctc ggacatggtc tcggaggcag ggatctcgga gattctggcc cagtcgccgg
    10081 acgacccgga gagggcagcg cgcggcctgc tctccgcggc cctcgacgct ggcggcaacg
    10141 acaacatcac cgtcgtcgtc gtcgacgtaa tggagcagcc tgcgcgcgag gcggacgcgc
    10201 gaaacgagcg tgagagacac tccagcggca tcagcgagct gctcgccgcg aaaccttcag
    10261 taaaggatac gggtgcccgc acgccatcca ggggcaaggc cgccccgagt cgcccccggc
    10321 ggcctaccag ggccgcgcgc cggaagcgac ctagtcgcct caggaaggcg ctcggaaagc
    10381 tcgtgcgggg gttggcgatc atcctggtcc tcgtcgccgc cctgacgccg gtctacctct
    10441 gggggtcaag caggtacttc ttcgagttcg acgagggtga ggtggtggcc taccagggtc
    10501 tgccctacgc tcccttcggc atcgagctca atcaggagtg gcgcaggcca ggcctgacgg
    10561 agtctcagat caaagagccc taccagaagc ccatagaaaa ccacaagctc tacaccaggg
    10621 accaggcaga gcgggtcatc gaggatctgg gcagctaagg tgacccaacg cgctttcacg
    10681 cccatgatcc tggcactgct cgtgaccact ctaggtttcg cgaccctcat cttcgtgcag
    10741 gaggcggcga gcccacccct catctatggc gcgtactacg cgggaaccct cttcgtgctc
    10801 tacctcggag tgaggctact tttgccccac tccgacgtgc ttctgctgcc catagtcacg
    10861 ctgttgacgg ggctggggct cgtcatgatc ttccgcctga catataacgt ggagggggcg
    10921 aagcagctcg cgctgacaca ggcggtctgg atcctcatgg ggagcggcgc catgttcttg
    10981 ctggtgctgt tcttccgcaa ctacgaccga atcttcgact acaagtacct gctcgccatc
    11041 gcggcggtct tcctgatcgc ctcgacgttc acgccccttg gctactcggt caacggggcg
    11101 cggctctggg tggatctagg accggtcaac tttcagccct cggagttcgc ccgcatcgcc
    11161 ctcgtgatct tcttcgccgg gtacctggcc gagaaacgtg acgtaatggc ggcgacgagc
    11221 cgcagcttcc tcggcatcca gcttcctgcg ataaagtact tcggacctgt agccctcgtc
    11281 tgggttgcct ccttcggcct cctcgtcttc gagaaggacc tagggtcgag cctgttgttt
    11341 ttcgccgttc ccctgctgat gctctacgcg gcgacgggcc gcaccgccta cgtgctgctc
    11401 gggggagtcc tcttcacgtt aggctccttc gtgaccttcc tcctattcga gcacgtgcag
    11461 ttgcgggtgc agagctggct ggacccgtgg agggaccctg acgggacggg gttccagatc
    11521 ctccagagca tcttcaacat cgctgacggc gggctgctcg gtacggggct gggggcgggc
    11581 ttcgcccaga ccatccccga ggtcgagacg gacttcgtct tctcagcgat agccagcgag
    11641 cttggcctgc tcggggcgac ggcagtcttg ctggccttcc tggccttcat ttacaggggg
    11701 accaagatcg ccctgctcgc agaggatgat gcatccaagc tcctggcctt cgggctcacg
    11761 gcgatgttcg ccctccagac cctgattatc gtgggtgggg taaccaaggc cataccgcta
    11821 acggggatca cgctgccctt cgtctcctac ggcggctcct ccgtcgtcgg cagcttcata
    11881 ctcacgggcc tcctcctgct cgtctcggag agggcgggac ggcgcgaggc cgagggcctc
    11941 gcctacaggg atgatccgtg gggggctcca tgaacgccca cctcaggcgt accttctacc
    12001 tgtttgtggc agggttcgtc ggactcgtgg ttatgctcgt gtactggcag gtctactcca
    12061 gggaggccct ggccaacaac cccgagaacg ggtttcagac ccagcgaagc gtctcctctc
    12121 cgcggggttt gatcctggcc ggcgacggcg agaccgtgct ggcgaagagc gtcaagcgcg
    12181 gcaccccgac aggccccgtc tatgaccgcg tctatcctct gggcgagccg ttcgcgaacg
    12241 tggtcggcta ctggagcacc cgctacgggg cgacaggcat agagatctcc aacaactccg
    12301 acctctccag cgtagcaggg gaaccggaga ccctggacga cctgatcaac cgcgccacgg
    12361 gaggcccgca acctggcaac aacgtggtcc tcaccctgga ccccgaactc caggagctcg
    12421 cctatcagga gctctcagcg agctccacgg gcaggggcgc cgtggtcgcc ttgaacccca
    12481 agaccgggga gattctcgcg atggccacat ccccatcctt cgaccccaac gatgtagacg
    12541 agaactatcc cgacctggct agtgccccaa acgcgcccct cgtcgaccgt gcggcccaga
    12601 gcctctaccc gcctggttcg gtattcaagg tgatcacggc cgctgcagca ctagagcaag
    12661 gagtcagccc gcagcagacc ttcttcgaca gcggggccta tgagttgccc gggtacaccg
    12721 tccgaaactt cgagggaaag gactacgggg aggtaacgtt cacgcgggcg ctcgcctact
    12781 ccatcaacgt ggtcttcgcc aagatcgcgg tcgaaatcgt gggggctgag gccctcacgc
    12841 agatggccgt gaacttcggc tttggggacc cctacgatga cttcccatta cccgtctcag
    12901 ggagcagcgt gaacagcctc ccacccgagc agtggaccac ggggaccctc gcccagacgg
    12961 gcttcggcca gggcgaggtg cagaccaacg cgttcgagat ggcgctcgtc gcaggagccg
    13021 tcgccaacga cggcgaaatc atggagcccc gcatcgtgcg cgaggtccgc tcccccgacg
    13081 gcatcatcct ggacaaacca acgccgagcg tccgcaggca ggcgctccca ccaggtacgg
    13141 ccaacacgct taacgggatg atgcaggagg tcatcatcca gggggggttc accgaagccg
    13201 agataccagg catgaaggtc gcggggaaga cggggaccgc cgagtcgggc aacgggctgc
    13261 cgcattcgtg gtgggtcacc ttcgccccag ccgacgaccc ggagatcgcc atgtgcgtga
    13321 tgatcgaaaa cggcggaagg gccgatagga gcgccatgcc cctggcggcc cgcctgatga
    13381 aagcctacct cgagggcgga gtgtgaacgt tgtccgcact cgcgtctata atctaggtcc
    13441 gtgcagcagc tcgtagacaa ccgttaccgc cttgtgaagt cgctgggcag cggtggcatg
    13501 gccgacgtat acctggcaca cgacgacatc ctggataggg acgtggccct caaggtgatg
    13561 agcaatcgct acgccagcga cgaggagttc gtggagcgct tcaagcgtga ggcccagagc
    13621 gcagcagccc tctcgcaccc gaacatcgtc tctatcttcg acaggggcga gtccgaggac
    13681 gggacttact acatcgcgat ggagtatctg cccggcggca ccctgaagaa ccgcatcgtc
    13741 aagcgcggcg cgctccccgc ccgcacggcg gcggccgtgg cgctccagat ggccgaggcc
    13801 ttgagggccg cgcacgagcg cgacgtcatc caccgcgaca taaagcccca caacatcctg
    13861 atcacaggtc tcggggacgt aaaggtcacg gacttcggta tagcacgcgc ggcggcgtct
    13921 tcgaccatga cgagaacggg ccacatcctg ggtacggccc actacatctc gcccgagcag
    13981 gcgatgggcg agccggtcgg tccggcgagc gacctctact cgctaggggt agtcctctac
    14041 gagatgctca caggcgaact tcccttcgac gctgagaccc ccctggggat cgcgatgaag
    14101 catgtcaacg gccacctgcg tcagccgatg gaactcaacc ctgcggtgcc agacgggata
    14161 aacgccataa cgctcaggct gctcgccaag aaccccgaag gccgctactc gagcgacgcg
    14221 gagctcatag aagacctcga gcgcgtctgt gccgggctcg acccctcagg cacgacgacg
    14281 gagatgatgc cccacgccgt tgcggcgacc acgcgcctca accccccatc tcccccacaa
    14341 aggattggcc gcaacaaggt tcggcgtagg agggctgcgc caccgcttct cgccctcctc
    14401 gcgctcctgg tcctcgccgc actcacctgg ggcgggtaca tccttcttca ggaccggcag
    14461 gcggcgcaga taggcgtccc agacctggtc ggcatgagcc tggatggcgc cagagaaaag
    14521 gtaggggacg atttcgaggt cgaggtcgca aagagcgtct ccggcgagag acccgtcgac
    14581 accatactcg gccagagtcc cagcaccgga aaggcggaga aaggctcgac gatctcggtc
    14641 aacgtcgtcg ggacgcgggt gtcggatgtg cccgacgtgg tgggctcggg gcgcgcggag
    14701 gcggagcaag cgctgaaggc cgccggcttc caggtcgagg tcgacgagcg ggagagctcc
    14761 ttcgatgacc agaaccgggt catgggccag gatccatcgg gcggtacgcg tgctgaggtg
    14821 ggatcagggg tgaccatcac agtcgggacc ggcccttcga gcgtgccgat cccgcggctg
    14881 tacggaaaga ccccggcgca ggccaagtct gttctcctgg gggcaggcct gaggctgggc
    14941 acccagaacc gggactataa cagtgacgtt gcagagggcg ttatctactt ccagaacccc
    15001 tcaggaggag agaacgtgga gcccggaacc gcggtcgacg tcaccgtcag cctgggtccg
    15061 gagcaggtcg aggtccctga gatctacggc cttagcgtgg cggcggccca gacggcgctc
    15121 tccaacgccg ggctgggctc cacccccatc gaggtcgagg gtgacgagcc ggcaggcacc
    15181 gcgcttgcga cggacccagg ggtcggcgcg atgctggatc caggctccac cgtgagcctc
    15241 tactacagcg caggtccccc tcccgctaca acggcctccc ctgatgcctc gcagtcgccc
    15301 aattccgaac catcgcaagg ccccgatcag gggacccagg acgaagggaa caccgacgcg
    15361 aaacccgccg ccgacaaaca accagccgat agaccgcaac gcaacgagcg cacgcaggat
    15421 aacagtggac cggacagcag tggtcaggac agtagtggtc agggcagtag tggatctggt
    15481 aacggcgtag ggttgggcac gaacgagggc caggggtcag gaagcgaagg gtaggatcta
    15541 gaggagctcc cgcctcacgt agtctccgag gtaccctgcg ggcgtctcca cgggaggatc
    15601 tagctcaacg aagctgtggc gtccgacggc gttctctaag agccagtcct cctcgacggt
    15661 cttggcacgc ccgaggtcgt agcctgtctc gaagtagacc gcgcgtagcc ctcccgaagg
    15721 aagctcgacc acaaggatag ggtactctcc gggagaggtc gcccggatcc tgacgctccc
    15781 ttgtgcctgg ccgccttcca tgactggagt ctatacgaga tccggagccg gcgggggctc
    15841 agaggggccg tgataaaatc acgggagcat gaagagacca tatgacattc atattcgtgg
    15901 agggagtcac catgtcagat cccatacccg aagacggagg aactgaggcc ggagcctcag
    15961 agggtatcgc cgacgaaccc ctgctcgaca ggctgccgcc cgaggatcta cctgaggagt
    16021 tgctcctgcg gcgtctcggg aggctcgaca ccaggcgcgc gctcgccgag atctgcagga
    16081 acgtgcctgc ccttgaagag atccttcttc caaggggcat acaggccgac gccctgacgc
    16141 gcaagtcagg cggcggcgcg ccgctcctgc ggggcctcgc gcgcaggatc tcccacgacg
    16201 cggctgcctg gggcgcgttc cgcaccgcta tccaggagca gatcccaccc gagaccttcg
    16261 aagccctgga aggtttctcc ccggaaaacc tcgaggaact caagcaggcc cacaccaccg
    16321 agggtttgct gctggccgcc ctaagcgcgg aagaggagcc cgaagagggc gtcgtcgaga
    16381 cgctcctgtc cgcctggcgg gaggagaggg gccaagccga gaaacgggcc tccgaagatg
    16441 cccgtgtcag ggatctggaa gccgaattgg agcgcttgaa acaggacaac gagcggctct
    16501 ctttcggagc cagggccgcc aaagagaggg ccgccgccct cgccgaagag gtcgaggtcc
    16561 ttaccggcga acgcacggat acctccgagc tggtcaggaa ggcagaggag agggcagcct
    16621 cggcgttgga cctgcggggc cagctcgacg agcgcatcga gacgctcgaa aggcgcagcg
    16681 cccagctcga gaaggcgctg gaaggcgagc gctcggccta ctccagggcg gccgagcgcg
    16741 tcgaggagtt gcacgaggag cttgggcgcg tggtcgagga gcgtgacagg ataaagaacg
    16801 ccctgcagga tgcccgcttt accgacagag gcttcggaga gctcctggtg cgggccataa
    16861 agaacgaggt cgcggccctg ccggcttcgc tcgacagcac cgcgcaggcc gcccgcctga
    16921 tggagttcat gggcaaggtg ctacaggcgc actccgacct gcgtagtcct ggggaacagg
    16981 gccgcaggac ctcggaggaa gaccccgacg acaaggaccc ttctccagcg agccgccgga
    17041 gaaagacaga tgggccggcg cgtagcgccg cgctcccacg ggtcggcggc tatgccaggc
    17101 cgaggccaac gctgtccttc cgcgccctgg ggggagctgg tgaggtgggt ggcagcagcc
    17161 acctcctcga cttcggcaag acgaggattc tggttgacgc ggggatcaaa ccggacggac
    17221 gcggccccgt aacgcccgac ttcggcgggc ttgatgggct cgatgcggcc gttgtaaccc
    17281 acgcgcacct ggaccactgc ggggcgctgc ccctgctagt gcgagaccgg cccaagatgc
    17341 cgatctactg tacgccccct tcggcgaagc tcataatcag cgcgctcaac gaccacgcgg
    17401 cgatgggcgg cgggctgccc ggaggcgcac ctatccatga ggtccgtaag cggctggtcc
    17461 ctgtaccctt cggcaagccg ctggatgtcg ggggggcgaa ggtcacgctg accgagagcg
    17521 gccatatcct gggggccgcg agcgtcctct tctcctcagg agaagcgaca gtcttccaca
    17581 ccggcgatat ctgcatggaa gaccacttct ccatcccctc ggcccgactt cccgaggtac
    17641 gggatataga cctcctcatt atggaggcga cactggccga ccagaagcca caaccgttct
    17701 ccgagtcgat caggacgatg gtcgaggtca taaatgatac gacgctggat cggggcggga
    17761 cggtcttgat ccctacgtac gccctcgggc aggcgcagga gatcatcctc ggcatcaagc
    17821 actacggcag ggagtacggc ctggatagag aagtctttat ctacgttgac ggttccgtgg
    17881 tgacgacctc cgagcggctc tacgccgagc agctcgggta tatgaagccc tacctccagc
    17941 acggcgaccc gcgtgagatc ttcttctcag agaacatcag ggccgtcgcc aacgacgaca
    18001 aggcgcgcga gcgtattctt tcgaacccct gcgccatcat cgcctccccg gtaacgatgc
    18061 agggtggggc gagcgctttc taccgcaagc ggctggagaa gagttcggag aacgccgtca
    18121 tcctgccatc caatgccgcc tcatcctacg gtacgcaccg cgccgagggc gaggaggagc
    18181 agtggagggt cgagcgggtc agcttcgccg cgcactgcac ccaggacgag ttgctcggca
    18241 tcaccgagaa actaagcccg cgtcagatca tcctgatcca cggctccagg cgccggataa
    18301 gcgacctggc cttcaggctc gccccaaacc acaagatcca caccccgacc gtcggcgaga
    18361 ccgtccgcac cgtgctgtag tgtcggggga ctgctcgagg tcatccggct cagggaagcg
    18421 aagggggggt gatcaccagc acctcggggt gaggctgcca aagctgtgac agcgcttttc
    18481 acgggagctg aactatgaac ctggtgcctg aagagttatc tcggccagcg cagcgccgtt
    18541 ggggcgcgcc ggccttcgag gagatccctc agggcgtaag gctcgtccgt ccagcttctg
    18601 ccagcgcccc ctgagaggga cgcaaggtcg atcaggcgtc ccaggatcct gaggcagaag
    18661 ccgttcaaca cgtcgtcggg atcgtcgacc cgcccacgcg cacgtgccac gaagcgtcct
    18721 ctggtcttcc acacgccgtc gcggtaaacg gcgaatatct ggcagggtgc atctccggcg
    18781 tccgtcgacc agccgaggat gcggcccgag tagcccgaag gctccaggat gtaccggctc
    18841 aactcctcgt gcatgaggtc ctcgtcggtc tcggccgccg cgccagacac gagcccgaac
    18901 gagacgccgg cctccgcgcc tggccccgcc acggcccgca cgtcgagcgc cccgcccgcg
    18961 taggcggtct cgacctcgaa cccggcctgc cgtccgagta ccgccgtata ctcgagcccc
    19021 gccgagatac gaccaaagaa gcggcgtgcc atcgtttcca cttctcgaac gagctcgagt
    19081 gatacgtcgg cctcttcgcg gtagcggcgc agacgggcgc gagcactctc ctcgaagccc
    19141 aagactctaa tccttcgccc aggccctgag aacgcggtct gcgagtcctg ggaagagcgt
    19201 aacaccggcg acgaagagcc gatcggggat cgtcacgtag acgtcgcggg gacctctttc
    19261 agcagcgtcc gctatctttt gtgcgactct ctctggccta acgcccttcg ggcgccagcc
    19321 acgcttctcg tccctggtgc gtctggagtt ctccctgaaa gacgtgctgg tcgtgcccgg
    19381 atagacgctg gtgacggaga tacccctgtc ggcgacctcc accctgagcg cgtcggagag
    19441 agcgttgagg gcgaacttgc tcgcgcagta ggcgccgacc ttggggatgg cccgcctgcc
    19501 gaccacggac gagacattga ttatgcgccc ccccttcccc atgtacacta aggcggactg
    19561 gatgcagttc agggggccaa tggcgttgac ctcgaacacg tgccgcacgt cctcggtccg
    19621 cacctcggtg atcctccctg agagtcctag cccggcattg ttgaccagta cgtccaggga
    19681 ccctagctcc ccgactgccc gtccgaccat ggcctcgacc gagccccttt ccgaaacatc
    19741 ggtctcgacg gccaccgcca cgccgcctga ggctgagatg ctacgctgca gcgcttcgag
    19801 tttgtccaca gccctggagg ccagcacgac agaagcgccg cgcgacgcca gctccctggc
    19861 cgtcgcctcg ccgatgccgc tcgaagctcc ggtgacgacc gccaccttgc ctttcaggga
    19921 tcgcgccata ggatgattat ctctggcgag cggggtgact tgctcccctg aagcccctca
    19981 ccaggacgga cgccagggcg ggaaaatgga tgggtcctgg tggcgcaggg agatgtcgta
    20041 gagctcgcgg aatatctcca tgacctcctc cttgcgacgc tcctcgcggg cagtcagcct
    20101 ctgcgatcgc ttcaagttct ccagccagcg caggctctgc atcttgctga ccactatcct
    20161 ccgtccttcc gttcctggcg agccttcttc ccttggggtg cttgcccctc catcgacagg
    20221 ggcacgtata gtaccatctc gaaccgtact tcagtcagcg tgccagcacg cctgcttcgc
    20281 aggctttcaa catttcagca cgctgcccgg cttcgccacg cttcttgcca gccgttcagc
    20341 tagctcatag atggcggagg gtactctgga gagtcgattt atagagataa aggacacgcg
    20401 atcgagcatg gacggtgaac cgccaccaaa aactgaaatg ccgaagtgct gacaatctgc
    20461 cgcaggcagc gtgctgacca gcttgcaaag aggttttacc cctttgcaaa cctagcgacc
    20521 gttaatgagc ctcgcagctt ccttacggtc actcaccccg aggaccatgt aggtggcgcg
    20581 catgtgttgc tttaccgtcg actcggaaag gaagaggttc ttggcgatct gtgcgttgct
    20641 gagtccctgg gcgacgagtt gcagtatctc ccgttggcgg atggtgagcg cgtcgagacc
    20701 cgtggggtca tcgttcgcta tgaggtactc gaggagcttc ctgggggcga cgatctcgcc
    20761 ctcgtgggcc acctccacgg cgcgggctat ctgatccaac tccatcccag cgtggatgaa
    20821 ccccctggct ccgtggcgta gggcggcccg ggcgagcggc agatccacgt gcaggctgaa
    20881 gaccagaatg gggatgtcgg cgttgacccc ccggatgcgt tctatgccct cagatatgcc
    20941 ctcggcgcca ctcgtgtcga atacggcaca atagggaacc tcggcgtccg taggagcggt
    21001 gtctccaacg tgtacccgcg cccgcccttc cagcgcctcc ttgagcccgg cggccacaac
    21061 gggataagag caatcgatcc agacaagccc caaacgctca cgctcgccgc ccaccaatgt
    21121 cgcctctctc cgtagaaggt cctgcagggc tgtacacgcc tatcaggttg cagtagaaca
    21181 ctgtttgcat cttaccagag aatgcctggg tgctaatacc ccgggttagc cctccatccc
    21241 gatcgactca ggcacaaccc ctggtctgca ctctgcctac acgtgtccgc cgccggatcc
    21301 tcgtctgagc cggctctgta tactacctga gtggccagcg tactcgtggg gataacgggc
    21361 ggcatcgcgg cgtacaaggc gccaggtgtg atccggcgcc tcagagaggc tggaaacgag
    21421 gtcagggtcg tcgccaccga tgccgctttc cgctttgttc ctgaagagac gctctccatc
    21481 gcagccggcg ccggcgtcca cacggacgtg acctggtggg agcattcggg ccgcgtcgaa
    21541 catgtctccc tcgcccgttg ggccgacctc gtcctcatag ccccagcgac cgcagactcc
    21601 atggcgcggg ccgccatcgg gctcggggac gacctgctct cggccacgat cctcgctggc
    21661 gcgaagagag tactttgggc cccggccatg aaccctgaga tgtggcagag cccagccaca
    21721 aagcgaaacg tcgatacgct gaagagctgg ggtcaccgct tcgtcggccc agccgaaggc
    21781 ctcatggcct cagcggagga agaacccggc gtagggcgcc tcgccgagga aacagagata
    21841 gtggctgccg tccgagagat tctcgcaacc tgatagctgg tcagcttgta gttttcgcat
    21901 acagtcgaat gcgcgggttg cagagaatcc atagtcctgc gaatcgccct agatccgtcg
    21961 caggtagtct ttcagggcct gctcggtgct cgggaaggcc atctccgtcc acgggagacc
    22021 ctctcgtggg aaacacctga tgtccatcgt ctcgtcgaga ggcgtaggct cgccgcccgt
    22081 gacctcggcc gtgaagacgg ctatggccgg tatctgtccg tcgtacgtgt agaggccgac
    22141 gatctccccc acctcgatct ccagccctga ctcctcgagc acctcgcgct ccgctgcggc
    22201 ctcgacccgc tcaccgcgct ctacaaaccc gccagggaaa gtccacttgc cgtagcccgg
    22261 ttcgatgtcg cgctggatca ggactatacg cccgtctatc tcgataatag cccccgccac
    22321 gagtttcggc cccaggtaga atacgaaacc gcaactctcg cacacgagcc gctccggttc
    22381 gccgcgtttg agcaagcgga cgcggaggcg gttgccacac ctggggcaga acttgtactc
    22441 ggagatcaga tactctactt ccaaggtgtt ctcaggttac tagggccacg tccggcgtgc
    22501 taaaataccc gcccacgcgg acgtagctca gttggtagag cgccagcttc ccaagctgga
    22561 ggtcgcgggt tcgaagcccg tcgtccgctc tgttatggca tgtcagcacg ctcaggctgg
    22621 cgccttcgct tgtcagcgct tccctttcca gtctcgctcg attgcgtatg gagagtcgcc
    22681 gtcggtggag atggggtcgg acgcccaggg gcgacttcct gacaagcgag ggccgaaggc
    22741 ccgcagcgtg ctgaaatgct gacaagcgaa cgaagggagc gtgctgacta gctcctcctg
    22801 atcttgcgca tcagccattt ggccgggtcg tagtactcgt cggcgacgcg ctccttgagg
    22861 gggatgatgg agttgtcggt gatgtggatg tcgaccgggc agacctcggt gcagcacttg
    22921 gttatgttgc agtacccgag gccgttgtcc tccttgaggt cggcaagccg gtcggcctcg
    22981 tccatcgggt gcatctccag ccactggttc ctgacgaaga agcgtggtcc tgcgaactcg
    23041 gggtgcttgt ggtgggttct caagacgtgg cagacatcct ggcacataaa gcactcgatg
    23101 cacttcttcg gttcgtagag tcgctcgaca tcctcctcgt acatggtgaa gggagcctgc
    23161 tcgctggtct ggaaaggctt gatgcccttg gacacctcgt agttccagga gacgtcggag
    23221 accaggtcct ttatgacggg aaaggcacgc atgggaccta cgtatatctc tcccccatcg
    23281 tactcctcga tgcgcgtctt gcacaaaagc ttgggcctgc cgtttatctc ggcgctacac
    23341 gagccgcagt gggccgcttt gcagttccag cgcaccgcaa gatcaggggc ctgttgggcc
    23401 tgtatcgcga gcacggcgtc gagtacgacc atgccctcga ccaggggtat ctcgtactcg
    23461 acctcctcgc cgccctccgc gtcgccgcgg aagatcctca gctttgccat cttgtcgctg
    23521 gtgcccagtt cctcgttcgg taccggggag tgggccttgg aggtcacccc ccctggatta
    23581 ttgccgttga cttcgctcat gccttcgcct tcgcctcctc tacgagcgac gccaggtgtt
    23641 caggcatggg cggtatctcg cgccgctcga tgccgacctc gccctgcccg tccttcttca
    23701 cgataaagtt gatcttcccc agctcctcgt caggaccgtc gaagtccaat ctccactgcg
    23761 ccccccgtct ctccttgcgc tccagggccg tcctgatgat gatctccgag atctggacca
    23821 gataacgtac gtcctgggcg gtgtgccatc cagggttgaa gagcctggat cccggaacgc
    23881 ttatttgcgg cagccggtcc ttgagcgcga gtatcttgcc aaggccctcc tcgagggagt
    23941 ccccgtcgcg catcaggttc gcatgctcca tcatagtctc ctggagctct tgctggatca
    24001 ggtaggggct ctcgccttcc tctttcttct ccaaaggctc gagcacacgt cctatctcgt
    24061 gcagcacctc gccctcgtca atctccgccg aatgcacgct ctcctcgatg tacgcggctg
    24121 cgccctcgcc ggccctacga ccgaagacga ggagatctga gagcgagttg cccccaaggc
    24181 gattcgcgcc gtgcagacca ccggcgacct cgccggcggc gaagagccct ggcacgctgg
    24241 aggcggcact ctcggggtcg atcttgatcc cgcccatcgt gtagtggacg gtcgggaaga
    24301 cctccatcgg ctccctcgag atgtctatgt cggcgagctt gaggaactgt tcgtacatgg
    24361 tcggcagctt cttcatgatc ccgtcgtagc cgaggtgcgt gatatccagg tagaccccgc
    24421 cgtgctcgct gccgcggcct tcctgtactt ctgtgtagtt cgcccgagcc accacgtcgc
    24481 gcgtggagag ctccatcttc tcggggtcgt agttctccat gtaccgcaca ccctcggagt
    24541 tgcggagcag tcctccctcg ccgcgcacgc cctcggtgac gagcagaccg cgcacgcctg
    24601 gaggccagac catccctgta ggatggaact gcatcatctc catgtccacc agctcggccc
    24661 ctgcgtcgta gccgaggacc acgccgtccc ccgttccctc ccaggagttg gaggtgacct
    24721 tgaagatgcg tccccaccca cccgtcgcca tgactaccgc cctggccttg aagtggacga
    24781 acttgccgtt ctcccgcgtg taggccaggg cgccgacgac cctgccttcc ttcttgaaga
    24841 gcttggtgac ggtcgtctcc atgtatactt tcgcgtcggt ggcgaggacc ttctcctgca
    24901 tggtccggat cagctccagc ccggtccggt cgccgacgtg gcagagacgt ctgtaggtat
    24961 gcccgccgaa aggccgctga cttatcttgc cctcaggtgt cctgttgaag agggcccccc
    25021 actgctcgag ctcgtagacc ctgtcgggag cctctttcgt aaacgtctcg accatcttcc
    25081 agttgttgag gaacttgccg ctcctcattg tgtcgataaa gtgctggcgc caggagtcgt
    25141 cggggtcgac gttcccgaca gaggccgcga tgccgccctc agccatgacg gtgtgggcct
    25201 tgcccagcag gctcttggtg acgatgccga ccgacaggcc gtgctcggcc gccgctaccg
    25261 cggccctgag acccgctcct ccggcgccga tgatcaggac gtcgtgctcg cgcgtctcgt
    25321 atgcctcacg gtgtccgttc tgtccatctc tctgcaactc caccacctac cccaaaatcg
    25381 tcaagtcggg tatgacgccc gcctgaatga gcctgaaata tacgtccgtg agcagcaccg
    25441 agaacaggct gtaccaggcc cattgcgggt gccgcaagtt caagcggctg atgccgttgt
    25501 aaaccttgcg cctggcgttg ccccccctaa cacacgtgta gcaatccttg ttgcccccta
    25561 ccaggtgccg cagcgagtgg cacgagaggc tgtacgtgga gagcaggatc acgttgacca
    25621 gccagatgat cgaacctacc ttaaccccga acgagcctcc ggggaagaag gttagaaccg
    25681 tgtcgtacca aaggaaggca agcaccacga aagaggcgta cagaaagtac ctgtggacgt
    25741 tgttgagcac gaacacggcc ctctcgcccc tgtagttctc ggccttgcgc ggctctctct
    25801 cctgcgcccc ggcctggcag gcaggcgggt cccagaagaa cgccctgtag tacgccttgc
    25861 ggtagtagta gcacgtcgcc cggaaaccca ggggcatcca gattatgaac atcggcgccg
    25921 ttatgaagtc aggcacccag tcagggtgtg ggatcggcgg cgagtaaaac ggcgaaagat
    25981 aatccttgta ctcgccaggc aaaaaagaca cgatgaggaa tgagtagaga ccgaaaacac
    26041 tcaatacgat cacaacagat accggctcga cccaccacgg taacctcttc caggcggcgg
    26101 ctatactcga caaccttgtt ctccctctag atacttgtaa cgtcttgtaa ctaaagtcgt
    26161 ttactgattc gggaagtata gaggatcgta tagaggctcg ttgtgaat
//
LOCUS       DASTKM010000090         9234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1429809, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000090 DASTKM010000000
VERSION     DASTKM010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9234
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429809"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="VFQ10_03555"
     CDS             <1..1444
                     /locus_tag="VFQ10_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET9964447.1"
                     /translation="VRERIERLLDPGTAFLELSSLAAYGMYDGRVPAAGIVTGVGRVS
                     GREVMVVANDATVKGGTYYPMTVKKHLRAQEIAEQNYLPCIYLVDSGGAFLPLQDEVF
                     PDREHFGRIFYNQAQMSAKGIPQVAAVMGSCTAGGAYVPAMSDEVVIVKGTGTIFLGG
                     PPLVKAATGEEVSAEELGGADVHTRRSGVADHFAENDEHALAIVRSIVENLNWEKSVP
                     WTVSEPEEPLYDPGELYGIVPSDYRQGYDVREVIARTVDGSRLHEFKPLYGETLVCGF
                     ARIMGHPVGILANNGILFSESALKGAHFIELCCSRGIPLVFLQNITGFMVGRDYEAGG
                     IAKDGAKMVMAVANAGVPKFTVIVGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGG
                     QQAANVLLTVQEEGLRREDKKMTDEEREDFKAPILEKYEDEGNPYHSTARLWDDGVID
                     PVDTRTVLALGLSAAANAPAEETRYGIFRM"
     gene            1447..1872
                     /locus_tag="VFQ10_03560"
     CDS             1447..1872
                     /locus_tag="VFQ10_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9964448.1"
                     /translation="MRAAVVIVENGRVALIERVRDGRTYFVFPGGRVEGGESPQQAAV
                     REAHEELGVSVDLGDLIFVAHRDGRERRYYLASIAGGTFGTGRGTEMMTSGTTAKGTY
                     RPVWVDLTILTELDVRPRELSEALASLAGAGEIRWIGEN"
     gene            1948..2688
                     /locus_tag="VFQ10_03565"
     CDS             1948..2688
                     /locus_tag="VFQ10_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9964449.1"
                     /translation="MTMNRPDSHNALNAALIEELTRCFEEISDDDGVRVVVLAGEGRS
                     FCAGADIGYMRETATFSYEENLEDARRLAMMYWTIDECPKPVVAKVQGAAMGGGAGLL
                     AVADVVVADSEARFAFPEISLGIAPATISPFVVRKIGVSHARSLFITGERFDAERARE
                     ISLVHRVVSRNGLDAEVDEKVGELLRGGPVAQATLKGLLRRLETTEPMEAPGLTARVI
                     SELRTGEEGQEGLAAFLEKREPRWRRER"
     gene            2685..4628
                     /locus_tag="VFQ10_03570"
     CDS             2685..4628
                     /locus_tag="VFQ10_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET9964450.1"
                     /translation="MSFGSVLVANRGEIAVRIIRTCRELGIRSIAVYSEADERAMHRR
                     LADEAYLLGPAPAPESYLNIGRLVEVIELSGAEAVHPGYGFLSESAEFARAVESAGAV
                     WVGPPPEAMETVGYKVQAKELARSAGVPTILGYEGEDNSEDRLAEEADRIGFPVLVKA
                     SAGGGGRGMRAVTHQRDFVEAVRGARREAEAAFGDGSVFLEKLVEDPRHVEVQVIGDR
                     QGNVLHLFERECSIQRRHQKVIEEAPSPALDPDLRESICNAAVRFACEAGYRNAGTVE
                     FLLDGDHFYFLEMNARLQVEHPVTELITGFDLLYLQLAVAAGEPLPLTQEEVTLRGSA
                     IEVRIYAEDEQGMPSGGLLETFSPPEGAGIRNDAGFEVGDEVPIHYDAMIAKLIVSAP
                     DRPSAVRRLRRALTDYEILGPSSNLPLLERIAAHPAFETGETTTRFLEDYGLTEPQPE
                     KPVPREAMILASAAELHLIPSSSDPFSAGPWRLLGATTLDYDEHRLEAVRTGPVRFKI
                     TAGGEMSVVEVLYYGDGRIRALIDEESLEAGILLGPEEVLISLRGETHTLRKPAPPAV
                     DETGPGADAAASLTAPMPGTVVKVLVQEGQEVEEGQLLLVLEAMKMEQPVSAPHAGVV
                     HSLPFDEGSLVPGGAVLAEVRET"
     gene            4856..5272
                     /locus_tag="VFQ10_03575"
     CDS             4856..5272
                     /locus_tag="VFQ10_03575"
                     /inference="COORDINATES: protein motif:HMM:NF044909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964451.1"
                     /translation="MSERTTEGATPDRAGIEGFFVRYGEAVSAGDLKAISGCYAVPAI
                     VLSDAGSIPISTREEIEAAFDGAAERYRAQGMVAARPTVVAVENLTEKLLFADVRWDY
                     LDEEGRSAERNSYRYVLRLVDAGPRICALIATSSGE"
     gene            5592..6281
                     /locus_tag="VFQ10_03580"
     CDS             5592..6281
                     /locus_tag="VFQ10_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9964452.1"
                     /translation="MGYAVRGLGRGSRPYGVLERLRALILTGAYGPDERLIEEQLAER
                     LGVSRTPVRQALTMLEAEGLVEITPNRGATVCSFSIEDVWDIYDLRAVLEGHAARRAA
                     GRIVRRELERLRDLAGEMEGLPGRFDDHEEEIRALVALNQEFHGTIAEASRNRRLERL
                     INRTVEIPLMFKAFFWYTPHERVISNLYHRQILESLENGDAGRAEITMREHVYEGRDF
                     VIGALEEDLNG"
     gene            6274..7488
                     /locus_tag="VFQ10_03585"
     CDS             6274..7488
                     /locus_tag="VFQ10_03585"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9964453.1"
                     /translation="MAEGDNGTRPLEGVRVVEMGSLLAGPFCGQLLADFGAEVIKLEP
                     PGKGDPMRVWGRHRKEGRTLWWPIIARNKKSVTINLREREGQDLARRLISGGDVVVEN
                     FRPGTLEKWGLGYEDLSRINPGLVMVRVSGYGQTGPYKDQAGFGSIGEAMGGIRHVTG
                     YPDRPPPRVGISLGDSLAATFGALGAVTALYNRETRGGKGQVVDVGIYEAVLALMEST
                     IPEYALAGHIRGRTGAILPFVAPSNTYPTGDGDYVVIGANADTVFGRFAKALGHPEWA
                     EGEKYATHHARGENQEELDALISDWTSQRSAEEVLEVMKEASVPAGKLFTAEDMLSDE
                     QYAARQNIVTVDDPEIGPFPMQNVVPRLSETPGGVRWTGPKLGQHNEEVFKEVLGMSE
                     EDLDGLRERGIV"
     gene            7488..8369
                     /locus_tag="VFQ10_03590"
     CDS             7488..8369
                     /locus_tag="VFQ10_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET9964454.1"
                     /translation="MHIEIVDVGPRDGLQNEDKTLSPEVRAELCEKLAAAGVPRVEAA
                     SFVNPKRVPQMAGAEEVMSAIERSPGTSYAGLVLNEKGYERAVQAGVDEVRYAFPVTE
                     TFAKRNQNTTVADAAQLAVRLVERARLDGVRVSITLSAAFGDPFEGWVDPERVLGIAA
                     SVAASGPDEIVLADTIGVGVPNQVRKLVEGVRAHGITVACHFHDTRNTGIANAAAAVE
                     SGATVLDASVGGTGGCPFAPRATGNIATEDLVYLLHGMDYETGIDLDALIEVATWLAQ
                     QLDKELPGQVYKAGNFV"
     gene            8560..>9234
                     /locus_tag="VFQ10_03595"
     CDS             8560..>9234
                     /locus_tag="VFQ10_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HET9964455.1"
                     /translation="MYNVAVDVGGTFTDVLVFDEETGGLTEGKVLSTPEDPSKGVVEG
                     IEAVCEKVGISFPDLHLFFHGTTVVTNMILTNTGSRVGLLTTKGHEQILHLARAWTPG
                     PLYGWMALQKPDPPADPTDTVGVSERISSGGEVLDELDETEVREALKRLVDRGVESLA
                     IGFLNSYVNPAHERRARDIVREAYPDLSVSISADIGQEYGEYERTLTTVVNTAVQPRT
                     MLYMRNF"
BASE COUNT         1838 a         2783 c         3081 g         1532 t
ORIGIN      
        1 cgtgcgggag aggatcgaac gcctcctcga cccaggcacg gcctttctgg agctctcgtc
       61 cctggcggcc tacggtatgt acgacggaag ggttcccgcg gcggggatag tcacgggggt
      121 tgggcgcgtc tcgggacgag aggtgatggt agtagccaac gacgcgaccg taaagggcgg
      181 tacctattac ccgatgacag tcaagaaaca cctgcgggca caggagatag cagagcagaa
      241 ctacctgccg tgtatctatc tcgtcgactc agggggtgct ttcctgcctc tgcaggacga
      301 agtcttccca gaccgagagc actttgggcg catcttctac aaccaggcgc agatgagcgc
      361 taagggcata ccccaggtcg ccgcggtgat gggaagttgt acggccggag gagcgtacgt
      421 ccccgccatg agcgacgagg tggtcattgt caaggggacg ggtaccatct tcctgggtgg
      481 tcctccgctt gtcaaagctg caaccggcga agaagtatcg gctgaagagc taggtggcgc
      541 ggacgtccac acccgccgat cgggagtcgc cgaccacttc gcagagaacg acgaacacgc
      601 cctcgctatc gtccgctcta tcgtagagaa cctgaactgg gagaagtctg taccctggac
      661 cgtctcggag cctgaggagc cgctctacga ccccggagaa ctctacggta tcgtccctag
      721 tgactacaga caaggctatg acgtcaggga ggtcatcgca aggacggtgg acggcagccg
      781 cctgcacgag ttcaagccgc tgtacggcga gactctggta tgtggcttcg ccaggatcat
      841 gggccacccc gtcggcatcc tggccaacaa cggcatcctg ttctcagaga gcgcgctcaa
      901 gggtgcccac tttatagagc tgtgctgctc ccgcggcatc ccactcgtgt tcctgcagaa
      961 catcacgggc ttcatggtag gcagggatta cgaggccggt ggcatcgcca aggacggggc
     1021 caagatggta atggcggtcg ccaacgccgg cgtgccgaag ttcaccgtga tcgtcggcgg
     1081 ctcgttcggg gccggcaact acgggatgtg cggcagggcc tactcgccgc gctttttgtg
     1141 gatgtggccc aacgcccgca ttagcgtgat gggcggccaa caggctgcga acgttcttct
     1201 caccgtgcag gaggagggac tgcggcgcga ggacaagaag atgactgacg aagagcgtga
     1261 agatttcaag gctccgatcc tcgaaaagta cgaggatgaa ggtaaccctt accactccac
     1321 cgcaagactc tgggacgacg gggtcataga tcctgtcgat acccgtacgg tcctggccct
     1381 cggactctcc gccgccgcaa acgcccccgc ggaggagacg cgctacggga ttttcaggat
     1441 gtgaggatgc gcgccgccgt cgttatcgtg gagaacgggc gggtggccct gatcgaacgt
     1501 gtgcgcgacg ggcgcaccta cttcgtgttt cctggtggca gggtcgaggg tggcgagagt
     1561 ccgcagcagg ccgccgtgcg tgaggcgcac gaagagttgg gggttagcgt agaccttggg
     1621 gatcttatct tcgtggctca ccgtgatggc cgcgagcggc gatactacct tgcgagtatc
     1681 gcaggaggaa cgttcggcac gggacggggt accgagatga tgacgtcggg gacgacagcg
     1741 aagggaacct acaggccggt ctgggtggat ctgaccatcc tgacggagct cgacgtgcgg
     1801 cctcgggagc tctccgaggc gctggcttcc ttggcaggag ccggagagat acgttggatc
     1861 ggagaaaact agtaatgccg ctacgcagag attggggtac cgatgcctga ctcgttgaag
     1921 gcgaccagga gtggcccctt ggcgaccgtg acaatgaaca ggcccgattc acacaacgcg
     1981 ctgaacgcgg cgctcatcga ggagttgacg cgctgcttcg aggagatctc cgatgacgat
     2041 ggggtgcggg tcgtggtgct tgctggcgaa ggacgctcat tctgcgccgg tgcggacatc
     2101 ggatacatgc gggagacggc caccttttcc tacgaggaga acctcgagga cgcaagacgg
     2161 ctggccatga tgtactggac catagacgag tgcccgaagc ctgttgtggc aaaggttcag
     2221 ggagcggcga tggggggagg ggctggtctc ctcgcagtgg ctgacgtagt cgtggccgat
     2281 tcggaggcgc ggttcgcctt ccccgagata agtctaggca tcgctccggc cacgatctca
     2341 ccgttcgtgg tgcgtaagat cggagtttcg cacgccaggt cgttgttcat caccggagaa
     2401 cgattcgacg ccgaaagggc gcgcgagatc agtcttgtac acagggttgt ttcgagaaac
     2461 ggactcgacg cggaggtcga tgagaaagta ggagagctac ttcgaggagg accggtcgcg
     2521 caggcgactc tcaaggggct gttacgccgg ctcgagacga cggagccgat ggaggcgccg
     2581 ggcctgacgg cgcgagtgat ctctgagctg cgcacgggtg aggagggcca ggagggactt
     2641 gcagccttcc tcgagaaacg cgagccgcgt tggagaaggg agcggtgagc ttcggtagcg
     2701 tcctcgtcgc caaccgcggc gagatagcgg tacgcatcat ccgcacctgc cgcgaactcg
     2761 gaataaggag catcgccgtc tactcggagg cggacgaacg cgcgatgcac cgccggctcg
     2821 ccgacgaagc atacctactc ggcccggctc cggccccgga aagctacctg aatatcgggc
     2881 ggcttgtaga ggtgatcgaa ctctcagggg ccgaagccgt gcatccaggg tacggcttcc
     2941 tctcggagag cgcagagttt gccagggcag ttgagtcggc cggcgcagtc tgggtcggac
     3001 ccccgcccga ggcgatggag accgtcggct acaaggtgca ggctaaggaa ctggctcgca
     3061 gcgccggagt gccgacgatc cttgggtatg agggggagga taatagcgag gaccggctgg
     3121 cggaggaggc tgatcgcatc ggcttcccgg tcctggtaaa ggcgagcgca ggaggtgggg
     3181 gacggggaat gcgggccgtg acgcaccaga gagacttcgt cgaggcggta aggggggcgc
     3241 gtcgggaggc cgaggcggcc tttggagatg gatctgtgtt cctcgagaag ttggtggaag
     3301 acccgcgcca cgtggaagtg caggtaattg gagacaggca aggcaacgtc ctccatctct
     3361 tcgagcggga gtgttcgatc caacgccgcc accagaaggt cattgaagaa gccccgtccc
     3421 cggcgctcga cccggacctc cgtgagtcga tctgcaacgc cgccgtccgc ttcgcctgcg
     3481 aggcgggcta ccgcaacgca ggcacggtag agttcctact cgacggtgac cacttctact
     3541 tcttggagat gaacgcgcgc ctgcaggtcg aacaccctgt gacggagctc ataactggtt
     3601 tcgacctcct ctacctccaa ctcgcggtcg cagcgggaga accactccca ctcacccagg
     3661 aggaagttac cctgcgcggc agcgccattg aggttcgcat ctacgcagag gacgagcagg
     3721 ggatgccctc gggcggctta ctcgaaacgt tctcgccgcc ggagggtgca ggcatccgta
     3781 acgacgccgg gttcgaggtc ggggatgagg taccaataca ctacgatgcg atgatcgcga
     3841 agttgatcgt ctccgcccca gatcgtccat ccgcagtacg ccgtctccga agggccctca
     3901 cagactacga gatcctgggt ccctcctcca acctcccgct cctcgagcgc atcgcggccc
     3961 atcccgcgtt cgagacgggc gagaccacga cccgcttcct cgaagactac ggtctcacgg
     4021 agcctcagcc tgagaaaccc gtcccccgcg aggctatgat cctcgcctca gcggcggaac
     4081 tccacctcat cccgtcgtca tcggatccct tctcggcagg cccctggcgt ctcctcggcg
     4141 cgaccaccct ggattacgat gagcaccgcc tcgaggccgt gcgcaccggg ccggtaaggt
     4201 tcaagatcac cgcaggagga gaaatgtccg tcgtcgaggt tctttactac ggagatggcc
     4261 gcatccgtgc cctcatcgac gaggagtccc tcgaggctgg aatactactt ggtcccgaag
     4321 aggttctcat ctctctacgc ggcgagaccc atacgcttcg taagcccgcc cctccagccg
     4381 tcgacgagac agggcctggc gccgatgccg ccgccagcct tacagccccg atgcctggta
     4441 cggtcgtgaa ggtgctcgtc caagagggcc aggaggtcga ggagggacaa ctcctcctcg
     4501 tcctggaggc gatgaagatg gagcagccag tctctgcgcc ccacgcagga gtcgtccact
     4561 cactcccatt cgacgaggga tccctcgtcc ctggaggcgc cgtgctagcc gaggtgcgcg
     4621 agacttagaa tccgtgcttc gctaaccccg gagggcacgc tcgccgtcgg ttggtgcaag
     4681 ccgtcctcct ccgtggcgcc ggacacaagc ccgcccgaaa agatcctctg gcgttccgcc
     4741 aaacagggcc aagtatgccc ggttttgtcg gtaccatcag ggcacatgga gtcgcggacg
     4801 ctgaggggcg cgacgggtta cgagctttgc aagccacgct aaaggaggaa ctccgatgag
     4861 cgaacgaacc accgaagggg caacaccgga tcgggctggc attgagggct tcttcgtccg
     4921 ctacggcgaa gccgtgtcag ccggcgacct gaaggccatc tccggttgct acgctgtccc
     4981 tgccatcgtg ctgtccgatg cgggcagcat cccgatctcg acgcgcgagg agatcgaggc
     5041 cgcgttcgac ggcgcggcgg agcgctaccg cgcgcagggg atggtggccg cccggccaac
     5101 cgtcgtcgcg gtggagaacc tcaccgagaa gctgctcttt gccgatgtgc ggtgggacta
     5161 cctcgacgag gaagggcgta gcgccgagcg aaatagttac cgttacgtgc tcaggctggt
     5221 ggacgcaggg ccgcgaatct gcgcgctcat cgcgacctcc tccggcgagt agcgtcccgc
     5281 cgtgtcgccg accttcacca cggcgctgat agagccgcgt gttagcgtcc cttctggttc
     5341 ggaaggttcg gcgggcggtt ccacgagcag agtctagcgc ggccgcctcc ggatggtctc
     5401 tgcaaaacat agagctatct acctgctgcg aatcagcaga atcgcacatt cggctacaaa
     5461 caaagacaac agccgacgag cggagcccga agggcgtagc gtgctgaaat gcttgcaata
     5521 ttgacacaag attcgtgggc gcctaagatt gtatacaagg gggtctccgt gggtcgaaaa
     5581 cgggggtttg agtgggatat gctgtacggg gccttgggag agggtctcgg ccatatgggg
     5641 tcttggagcg gttgcgggct ttgatcttga cgggcgcgta tggtcctgat gagcgtttga
     5701 tcgaggagca gctcgcggag cggctcgggg tgagcaggac ccctgtgagg caggccctca
     5761 ccatgctcga ggctgagggg ctcgtcgaga tcacgccgaa ccgtggggcc acggtatgtt
     5821 cattctccat cgaggacgta tgggacatct acgacctgcg cgccgtactg gagggccacg
     5881 ccgcgcggcg cgcggcagga cgcatagtga ggcgcgagct ggagcggctg cgggacctgg
     5941 ccggggagat ggagggtctg cccggccggt tcgacgatca cgaagaggag atccgggctc
     6001 tcgtcgcgct caaccaggag ttccacggga ccatcgccga ggcgagccgc aacaggcgtc
     6061 tggagcgcct gatcaaccgc accgtcgaga taccgctcat gttcaaggcc ttcttctggt
     6121 acacgccgca cgagcgggtg atctccaacc tctatcaccg ccagatcctg gaatccctgg
     6181 agaacggcga cgccggcagg gcggagataa ctatgcgcga gcacgtctac gagggccgcg
     6241 acttcgtcat aggagccctc gaagaggatc tgaatggctg agggtgacaa tgggacccgg
     6301 ccactcgaag gcgttcgcgt cgtcgagatg ggcagcctgc tcgcaggccc tttctgcggc
     6361 cagctcctcg cggacttcgg cgccgaggtc ataaaactcg agccgccagg caagggggac
     6421 cccatgcgcg tgtggggccg ccaccgcaag gaggggcgca ccctctggtg gcctatcatc
     6481 gcccgcaaca agaaatccgt caccatcaac ctgcgggagc gggaggggca ggaccttgcc
     6541 cgccgtctca tctccggggg cgatgtcgtc gtcgagaact tcaggcccgg cacgctggag
     6601 aaatggggcc tcgggtacga ggacctgagc aggatcaacc ccggtctcgt catggtgcgg
     6661 gtttcgggct acggacagac aggaccatac aaggaccagg cggggtttgg atccatcggc
     6721 gaagccatgg gcgggataag gcacgtgaca ggatacccgg accgtccccc gccgcgggtc
     6781 ggcatcagcc ttggcgactc gctcgctgcg acgttcggcg cccttggcgc ggttacggcc
     6841 ctctacaacc gcgagacgcg tggcggcaag ggccaggtgg tggacgtggg gatctacgag
     6901 gccgtcctgg ccctcatgga gagcacgatt ccggagtatg cgctcgccgg gcatatccgc
     6961 gggcgcaccg gggccatcct gcccttcgtg gcgccttcga acacctaccc taccggtgac
     7021 ggagactacg tcgtgatcgg cgccaacgcc gataccgttt tcggacggtt cgccaaggcg
     7081 cttggacatc ccgagtgggc cgagggcgaa aagtacgcaa cccaccacgc ccgcggcgag
     7141 aaccaggaag agctggacgc tttgatctcc gactggacca gccaacgaag cgcggaagaa
     7201 gtgctcgaag tcatgaagga ggcgagcgtc cccgcaggga agctcttcac cgccgaggac
     7261 atgctctcgg acgaacagta cgccgcccgc cagaacatcg tcaccgtcga tgacccggag
     7321 atcggaccgt tcccgatgca gaacgtcgtt ccccgtctct cggagacacc aggaggggtg
     7381 cgctggaccg gcccgaagct aggccagcat aacgaggagg tgttcaaaga ggtgctcggg
     7441 atgagcgaag aggacctgga cggtttgcgc gagcggggca tcgtctgatg cacatcgaga
     7501 tagtcgacgt aggaccccga gacggtctac agaatgagga caagacgctc tcccccgagg
     7561 tccgcgccga gctctgcgag aagctcgcag ccgccggcgt cccgcgcgtc gaggcggcca
     7621 gcttcgtcaa cccgaagcgc gtcccgcaga tggccggcgc ggaagaggtg atgtcggcca
     7681 tagagcgttc cccgggcacg agctacgccg gtctcgttct caacgagaag ggctacgagc
     7741 gggccgtcca ggcaggagta gacgaagtac gctacgcctt ccccgtaacc gagaccttcg
     7801 ccaagcgcaa ccagaacacc accgtggcgg acgcggcgca gctcgcggtg cgactcgtgg
     7861 aacgggccag gctcgatgga gtgcgcgtct ccatcacgct ctccgccgcc ttcggagacc
     7921 cgttcgaagg atgggtcgat cccgagcgag tactcgggat cgccgccagc gtagcggcat
     7981 cgggtccgga cgagatcgta ctggcagaca ccatcggcgt aggagtccca aaccaggtgc
     8041 ggaagctcgt agagggtgtc cgggctcacg gaatcaccgt cgcctgccac tttcacgaca
     8101 cacgcaacac aggaatagcc aacgccgcag ccgccgttga gagcggcgcg acggtgctcg
     8161 atgcctccgt cggcggcacg ggcggctgcc ctttcgcccc ccgcgccacg ggcaacatcg
     8221 ccacagaaga cctcgtctac ctcctgcacg gcatggacta cgaaacagga attgacctcg
     8281 acgccctcat cgaagtagca acgtggctcg cacagcaact cgacaaagag cttccaggac
     8341 aggtgtacaa ggcgggaaac ttcgtgtaag gcttcaggcc acagccatca cgcaaaacgt
     8401 cggacgtgaa caggaaaggg cgagcagtat gacacatcca gacacaaaat ctgaagaagg
     8461 tagcacacaa gaaaagccgc ctcgccaccc accagaaagc tgacaagcga aggcgaaacc
     8521 tgagcgtgcc gaaatgctaa tcgaacgtag ggagcgacca tgtacaacgt tgcggtagac
     8581 gtcggtggga cttttacgga cgtgctcgtc ttcgatgagg agaccggtgg gctgactgaa
     8641 ggcaaggtgc tctcgacgcc ggaggatcct tcgaagggcg tggtcgaggg gatagaggcg
     8701 gtctgcgaga aggttgggat ctcctttccc gacctgcacc tcttctttca cggcacgacc
     8761 gttgtgacga acatgatcct gaccaacacc ggctcgcgag tcggcctcct gacgacgaag
     8821 gggcacgagc agatcctgca cctcgccagg gcctggaccc ctggtccgct ctacggctgg
     8881 atggcactcc agaaacccga ccctccagcc gatccgacag acaccgtggg cgtctccgag
     8941 cgcataagct cgggtggcga agtgctagac gagctggatg agactgaagt tagggaagcc
     9001 ctaaagcggc tggtggacag gggcgtggag tcgctagcca taggtttcct caactcgtat
     9061 gtgaaccccg ctcacgagcg gcgcgccagg gatatcgtgc gcgaggcgta cccggatctc
     9121 tccgtgtcta tctcggcgga catcggacag gagtacggcg agtacgagcg gacgctgacc
     9181 acggtcgtca acacggccgt gcagccgcgc accatgctgt acatgaggaa cttc
//
LOCUS       DASTKM010000091        20313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_372435, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000091 DASTKM010000000
VERSION     DASTKM010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 20313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20313
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372435"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFQ10_03600"
     CDS             <1..395
                     /locus_tag="VFQ10_03600"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HET9964456.1"
                     /translation="AVSTTGLNPPTPLANFLDLLGGTAGPCALFALGATLAARTVAGG
                     TAEVSYMTFMKLALHPAALFFTTTIIFSVDPFWATAALLGAALPIAANVFIVARQYDV
                     YVVRTSSAILVSTAVSMISVSALLLVLQ"
     gene            561..950
                     /locus_tag="VFQ10_03605"
     CDS             561..950
                     /locus_tag="VFQ10_03605"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HET9964457.1"
                     /translation="MASIRETIEREVKGWPGVEARVHRFGGVEFRVNGHEIGHLHGDR
                     MADLPFPVRMREELVESGKAQLHHVLPQTGWVSYYLSGEEDVAGALDLFKKNYVRLTA
                     RKSAGTAGRAGHRGASDISEVDDPGKE"
     gene            1133..2554
                     /locus_tag="VFQ10_03610"
     CDS             1133..2554
                     /locus_tag="VFQ10_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9964458.1"
                     /translation="MSAEEVATEKVEVSRRLLIGVLISAVFVSVLNSSMVNVVVPVIG
                     RDYGVTEAQVGWVITGYLLTYAVGIPLYGRAADVFSIRSAFMVGLIAFAAGSLICAWA
                     PSLGALVFGRIVQGAGGAAIPALASVSVTKVLPPGERGAALGLIVSSVGIGAAIGPIL
                     GGAVGALAGWHYLFVGTLILTAALIPAALYVLPGDSPEDARGFDLPGGLLLGLAAGLF
                     LFGITRGQVSGFGSAGSWGCFAGSALAAAGFGWRIRNAAEPFVSPDLFQNREYVAAVV
                     VGYFCMLTNVSALVFVPLLVSEANGLSPGQAGLVLTPGAVALAIISPLAGRWSDSIGV
                     RPLIFAGLVTMLLSVLTISTFGAGASPVVVSLGMLGVGAGFALANSPNVNAAASSLGP
                     DELGAGLGIFNGAFFLGGGTGPAVIGAFLAARKEAEAGALNPLYGLDTAAFSDAFLVL
                     ALTLLISLVAALGLRNTTAKEKT"
     gene            2551..3441
                     /locus_tag="VFQ10_03615"
     CDS             2551..3441
                     /locus_tag="VFQ10_03615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9964459.1"
                     /translation="MSDYGRELEFGVSVAPLADPPDLAARVAKAADSAGLELVGIQDH
                     PYQRRFLDTWTLISTLVPVTEHIHFFPDVVNLPLRPPAMLAKAAASLDVLSAGRVEIG
                     LGAGAFWDAVAGMGGPRRSPGEAVRSVEEAIKVMRLVWSDERSLRFDGEFYSLKGMRP
                     GPHPVHDIGIWVGAYGPRMLDLIGRLADGWVPSFGHAPPGRLPEMNRRIDEGAERAGR
                     QPRDIRRVYNVSGSIGAVDEGSLQGPPSRWVETLTGFALEHGMDTFIFWPSGDHERQV
                     AVFAGEVVPAVREAVRSGRS"
     gene            3486..3794
                     /locus_tag="VFQ10_03620"
     CDS             3486..3794
                     /locus_tag="VFQ10_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964460.1"
                     /translation="MLDKKAYLKAARHHARRAGVSLLSARSPHSIMSFLSVGCILSAG
                     SILSIGSVGSVLSIGSSGSILSIGSTGWILSIGSVGSILGLTSAGYSPSREREDEEES
                     "
     gene            4023..5018
                     /locus_tag="VFQ10_03625"
     CDS             4023..5018
                     /locus_tag="VFQ10_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET9964461.1"
                     /translation="MSEGILQRLSRGIVLGDGGYLLELERRGYVQAGPFVPEPSITNP
                     EALEELHREFVDAGVDVLQTLTFYASEDKLATVGLEGRVDDMNREATRIARRIAGEAE
                     RDVLVAGNLSCAWVYRPEDPETHRRTRELFDRQLDVMTAEGVDFVIAETFNWLGEAMI
                     AAEAIRATGLPAMVTMGPDNDPNSFEGYSPGEQVKRLADAGVDIVGANCFRSPEYMLP
                     IMEEMRAAVPDAVHMAAQPVAFHGTDERPNYVSLPEFPYELEKITLPRATMGEFALRA
                     RDLGYNYIGSCCGSVAAHVREMARALGKLSSDEREWRTRTGIAQSGYELHRMREQ"
     gene            5084..6271
                     /gene="solA"
                     /locus_tag="VFQ10_03630"
     CDS             5084..6271
                     /gene="solA"
                     /locus_tag="VFQ10_03630"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HET9964462.1"
                     /translation="MQGTGKKAFEYIVVGCGGIGSAAVYWLSRAAGSEVLGLEQFHLG
                     HDRGGSEDHSRIIRLSYHDQAYTTLTPHTYQAWSEIEDESGIRLVFETGGLDLESVED
                     APDYINHYAGAMAAVGIPYQELSAQEVMDRFPQFILDESVLGVYQPGAGFVDAGRANA
                     VHIALARTRGATVLDEAPVYGVRTAGEGVEVETAAGTFSARKLVVAAGAWTNRVLGHL
                     GYGIPITCTQEQVTYFRTPHLWEFAPDRFPVWIWHGGPGYTFYGLPVYGAAGSKAGQD
                     TGGDVVSVDTRTFDPNPRILATLRDFLQRYIPRSLGPELYTKTCLYDMPPDREFVLGT
                     VPGIPQVAFFCGAGHAFKFAGLVGRILSEIALEGRTRYEIDAFRPEREALTDPDYRPT
                     LAM"
     gene            6278..7804
                     /gene="hutH"
                     /locus_tag="VFQ10_03635"
     CDS             6278..7804
                     /gene="hutH"
                     /locus_tag="VFQ10_03635"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET9964463.1"
                     /translation="MTEFYVENLDPASVVAVARQRARVAMGEEARERVSASRGRIEIM
                     LEGDRPVYGVTTGFGALANTRITAEKRLELQHTLLRSHAAGMGPFIEPEVVRAMMAIR
                     AKTLSAGYSGVRPELIDALVAFLNADLTPAVPEYGSMGASGDLAPLAHVGLCLVGEGR
                     LLEGGEPVKTSEALERAGIRPLGLATKEGLALINGTDGMLAMLLLSLEDLRVLLKTAD
                     VTAAMSVEAMLGTDRVFAEDLHSLRSHPGQLASAGNISKLLSGSPIVASHRDSPHLVQ
                     DAYSLRCAPQVFGAARDTLDHASLVAGRELASVTDNPVVLPNGRVESGGNFHGEPLAL
                     VLDFLAIAASEVGAITERRIDRMLDPRRSDGLPAFLSPRPGVNSGFMMPHYTAAALAE
                     ENRRLANPASTGSLPTSAMQEDHNSMGWSAGRKLRRLLDNVSGILAVEAACAAQALDL
                     RYPLQPGHATSAIRQRIRRDIPFIEGDTFMAPHLETAKSLVTSGELALTAERAIGPLA
                     "
     gene            8022..9704
                     /locus_tag="VFQ10_03640"
     CDS             8022..9704
                     /locus_tag="VFQ10_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9964464.1"
                     /translation="MSLLLVSGANLATPLLIGRAIDEGIGPRRLEVIFVVVGWLVGLA
                     LARALFTFLQGYLAERASQGVAYDLRDALFERIERLSFSYYDRVQTGQLVTRLTSDVE
                     QIRTFAGSGVVQLANAFVMLIGTTVLLLYLDWQLALVALAIVPIIAVLLVRFVGRIRP
                     LFREVQQTLGRLNTVLQEDLLGVRVIRAFAREDYETARYTSVNEELLQKNLTTVRVFS
                     NNFPFVFLFANLGTLAIIWFGGWQVIGGRLSIGDLVAFNTLLGFMLFPILTIGFLSAS
                     FSRAGASSQRVFDVLDAPLDVKDAPDASILLPLSCRVDFDDVSFRYPGSARDILAGVS
                     FTARPGQTVAVLGTTGSGKSTLVNLIPRFYDVTGGAVRLDGNDVREVTLSSLRSQIGI
                     VLQETRLFSGTVRDNVAFGKPEATDEEIVAAAEAAQAAEFVKELSDGYDTVIGERGIG
                     LSGGQRQRIAIARALLIDPRLLILDDSTSAVDAETEAAIQETLDRLMREKHRTVFVIA
                     QRVSTVRDADLILVLDKGSIVASGTHEDLRRESELYNEILGSQLLSPSETAA"
     gene            9795..10343
                     /locus_tag="VFQ10_03645"
     CDS             9795..10343
                     /locus_tag="VFQ10_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET9964465.1"
                     /translation="MGGTTGGQNARDCLSLLRTLRAVRSFRPDHVPQEVVDDVLEVAR
                     WSGSASNKQPWEILVVRERDTLRSLASVGGYAWHLAGAPLGIVLVMAGERAEQETYDE
                     GRLAERIMLAAHAHGVGSSIGWFVGSGRDAARELLGIPKEKVVRTAISLGYPEEEARR
                     SRARRGPARKSLGEIVHEESYA"
     gene            10351..10731
                     /locus_tag="VFQ10_03650"
     CDS             10351..10731
                     /locus_tag="VFQ10_03650"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HET9964466.1"
                     /translation="MNDTFDAETLRLLDETREVRIETRRENASPEHRTIIWVVVVEGE
                     VFVRSLRGPRGRWYREISSNPEGALHVHDDRLPVHAAHATEGAIVDAVSAAFRSKYQQ
                     SSPASTEAMVRPETLPTTLKLSPA"
     gene            10801..12585
                     /locus_tag="VFQ10_03655"
     CDS             10801..12585
                     /locus_tag="VFQ10_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9964467.1"
                     /translation="MPQERASDRGATAMRLLGELRPYVGKLVLVLGLVVLGAAAQAGG
                     PWLIGRSIDRYILPGDVAGLSRIMVVLFGVYAVGAIASRGQVYQVGSIGQSILASMRA
                     RIFDRLQHLPLGFFDRRPVGDLMSRVTNDVDTLNQLFSQGLTQLLGSLFSLIGIVIAM
                     LFLDWNLALACFAIIPIMLLLTSFFARRARRAFRLTRETVGDVTAGVQEDIVGVREAQ
                     AFNRTDENIARFRRRNAANRDANVQAVAITSAFAPTIDLLSTLATALVIGYGGYLVFA
                     GSLSIGILTAFLIYVQQFFRPIQLASQVYTQAQAAVAGAERIYNILDEEPEPEDLQDA
                     PKLGAASGRITFEHVTFAYDPGNPVLHDVDFRVEPGMTVALVGPTGAGKTTIASLIPR
                     FYDATQGRVLIDGRDVRELARKDLRANIGIVLQEPFLFSGTIAENIGYGRSGDPSEAT
                     REEVEAAASVVNADDFITSLPEGYDTVLGEGGGALSQGQRQLLSFARAILTDPRILIL
                     DEATSNIDTRTEATIQRALAKLLEGRTSIVIAHRLSTIRNADLILVIERGSIAERGSH
                     AALIEKNGLYADLYRRQFREPVPSGGRS"
     gene            12659..13531
                     /locus_tag="VFQ10_03660"
     CDS             12659..13531
                     /locus_tag="VFQ10_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9964468.1"
                     /translation="MSVLPERILLPTDGSKDAVRAAEAASDLAKRCGAELHVVHVWHD
                     VRGFAHDFVKRELRRQGQEILDEQVKKIRASGVEVTKAYLRRGRTSNEVIALSKEIDA
                     DLLVVGSRGLGTVRRILMGSQSEEIVHHAQVPVLVLTGAEDFWAPARIIIGEDFSDDA
                     RRAGELAAGIGKLYGAEGLLVYSHPDLPEVPPGEARSAVQNLTQMRERDKSMLEERAS
                     ELEEILGRRPEIRISGGYPANVLLEASQVVQPSLVAVGSRGLAGLIRTRLGSVSTKVV
                     TAARGPVLVYPHVD"
     gene            13682..14632
                     /locus_tag="VFQ10_03665"
     CDS             13682..14632
                     /locus_tag="VFQ10_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HET9964469.1"
                     /translation="MMPQTTGIHHVTAISGVPQRNVDFYAGTLGLRLVKKTVNFDDPE
                     TYHLYYGDGAGCPGTIMTFFPWAHAPGGRIGAGQLVVTSFSIPTSSLGYWTERLVENG
                     VRFERPRDRFGDTVLSFEDPDRLRVELVATEDGRPGWSGGPVQAEHSVRGFHHVTLAV
                     EAPDRTAGLMTDSLGFRSLDEVEGRIRLASGEGGPGYLVDVVNAAGFPHGSMGVGTVH
                     HVAFRVPDEETQLALREEVAALGYSVTPVLDRNYFRSIYFREPGGVLFEIATDPPGFA
                     VDEDPEHLGEDLKLPPWLETRRDRLEEVLPPLRVPIGESA"
     gene            14629..15285
                     /locus_tag="VFQ10_03670"
     CDS             14629..15285
                     /locus_tag="VFQ10_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9964470.1"
                     /translation="MSADLGFVHRYLPGEDESSPTLLLLHGTGGNEEDLIPLGQELAP
                     GAAFLSPRGKVSEYGAPRFFRRLAEGVFDQEDLLLRTHELAEFVEAASAEYGFDRSKL
                     VAVGYSNGANVAASTILLHPGLLKAAVLFRAMVPFEPDLTPDLSGMPVFLAAGRMDRM
                     IPPDNTQRLADILDEAGADVDLRWRDTGHPLTYEEVGEAKEWLSEVLPKFSSAQDRGK
                     "
     gene            15411..17072
                     /locus_tag="VFQ10_03675"
     CDS             15411..17072
                     /locus_tag="VFQ10_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9964471.1"
                     /translation="MAKPVILAVDDDPQVLRAVERDLRRRYAREYRILRADSGESALD
                     TLGKLKLRGDPVALFLVDQRMPKMTGVEFLEQAIERYPEAKRVLLTAYSDTEAAIRAI
                     NDVGLDYYLQKPWDPPDENLYPNLDDMLGDWQADYRPPFTGLRVVGDRWSPDSHRTRE
                     FLALNRVPYRWLDIEGSEEAREILAHADSGAPKLPLVVYEDGSYEEAPQNREIAEKIG
                     LQTRAGQQFYDLVIVGGGPSGLAAAVYGASEGLKTVLIEREAPGGQAGMSSRIENYLG
                     FPAGLSGGDLARRAVDQAKRFEVEILTPQEVSEVRAEDPYRIVTLADGTEISCHALII
                     TTGVSYRKLDVPGVDHLTGRGIYYGAAQTEALSCKGEDVFIVGGANSAGQAAMFFSKQ
                     AGRVFILCRGSDLRKSMSDYLVRQIEDMDNIEVLVDTGVVSVEGEDHLERITAKNTRT
                     GETETATAHSLFIFIGAAPQTEWLDGVVERDERGFILSGPDLMQGGKRPKGWRPDRDP
                     FLLETSVPGIFVAGDVRHGSVKRCASAVGEGAIAIQFVHQYLREI"
     gene            17074..18102
                     /locus_tag="VFQ10_03680"
     CDS             17074..18102
                     /locus_tag="VFQ10_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9964472.1"
                     /translation="MREASDEFVSQQHGKLISLGTMAAGLAHELNNPAAAIGRSAAEA
                     REAFREASEKAAELGTLPLTEEQHSFVAGLPEEVARRTAASLDPLEVSDLEDELAMWL
                     EDRSVEEGWEISSTLAGAGLETAWLEGLADRVPQEALGGVLGWLRATMAGDELLREIE
                     GGSARISELVGAIKTFTHMDKAATRKVDVHAGLNSTLIMLGHKLKKGDVEVVRDYEKD
                     LPHVCGHASELNQVWTNLLDNAIDAVDGQGRITVRTATENGRVLVEVSDDGPGIPDDV
                     RERIFEPFYTTKDVGEGTGLGLDITYRVIVEDHKGDIRVLSEPGDTRFQVRLPVNEPG
                     DAEKAADV"
     gene            18099..19472
                     /locus_tag="VFQ10_03685"
     CDS             18099..19472
                     /locus_tag="VFQ10_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9964473.1"
                     /translation="MMREVREEQGFTPEQVEALMPYGRESRLQTGDLLFDERSVVDSF
                     YVVLEGEVEITRLDGAAEIPVQTHAPGEFTGGLAVLTGRTSIHRGRAGAPSRVLEIDS
                     ETFRRMPAEVPDLADIFISGLARRMRYTQRAYRQREKLAALGKLSAGLAHELNNPAAA
                     ARRASEELGEAILEAQLTSVGHDERFSAGEREALVALQRETAAGNAALLDPLSLGDAE
                     DRLADWLEEHGCEEPWDMAPALAEAGLDTDRLEAMAGAFDDRSLACGLEWLAGTLDLL
                     GLASEIETSAGRISELVGAMKEYTYMDRAVVGEVDVISGLQNTLTILGPRLKDVTLLR
                     EYEKDLPGIPGRGGELNQVWTNLIDNAIDAVDGRGSITIRAYVEGDRVVVEVVDDGRG
                     IPREAQVHVFEPFYTTKDIGAGTGLGLDIVRRIVTGHGGEIFVQSEPGETCFTVRLPL
                     DTTQNGG"
     gene            19478..20065
                     /locus_tag="VFQ10_03690"
     CDS             19478..20065
                     /locus_tag="VFQ10_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HET9964474.1"
                     /translation="MGKTHVYEEICTQKREVDARVLEQTVSLAVEIAREGREGRKIGT
                     LFVVGDSGEVIKRSKPLILDPLHGHPDESKQIEDPDMRETIKELAQLDGAFLVSNAGV
                     VLSGARYIDAASDSLDLPLGLGSRHVAGASISRQTRAVAVVVSESSRVRMFDDGELVS
                     EIVPELWMIDGYTSRMEGRTFTRRDEDVTVIGRAE"
     gene            20055..>20313
                     /locus_tag="VFQ10_03695"
     CDS             20055..>20313
                     /locus_tag="VFQ10_03695"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HET9964475.1"
                     /translation="MPSKADEVLDFWFGRASEPGYGEFRDEWFRKDPEFDARVTDQFA
                     GLYEEAAAGDLDGWRDDARSCLALVIVLDQFPRNMFRGDGRT"
BASE COUNT         3726 a         6436 c         6616 g         3535 t
ORIGIN      
        1 tagccgtctc gaccacagga ctcaaccccc caacgcccct ggccaacttc ctcgatttgc
       61 tcggcggcac ggcaggcccc tgtgccctgt tcgccctcgg cgccaccctc gcagcacgca
      121 ccgtggcggg cggcacggcc gaagtctcct acatgacgtt catgaagctc gcactccacc
      181 cggccgccct cttcttcacg accaccataa tcttcagcgt cgatcccttc tgggctaccg
      241 cagccctgct cggtgcagcc ctgcccatag ccgccaacgt ctttatcgtc gcccgccagt
      301 acgatgtcta cgtcgtccgt acctccagcg ctatcctggt ctctacagcg gtgtccatga
      361 tctctgtctc ggcattgttg ttggtcctcc aatagggcgg aaaaccgtac caaggtccag
      421 gtgggactcc acctttcgtc cggtgtaata atagaccctc gctcatatga tgtgggcaca
      481 catcaaggct tgatatcaac ctcgtcggtg gtattgcttg tttagccggc caagcagagg
      541 cggtaagaga ggagggcaac atggccagta tcagggagac catcgagcgt gaggtaaagg
      601 ggtggcccgg ggtcgaggcg agggttcacc ggttcggtgg ggtcgagttt cgggtaaacg
      661 ggcatgagat cgggcacctg cacggcgaca ggatggccga cctccccttc cctgtgagga
      721 tgagagagga actcgtcgag tcaggcaaag cgcaacttca ccacgtgctg ccccagacag
      781 ggtgggtcag ctactacctg agtggcgagg aggatgtcgc cggggcgctg gacctgttca
      841 agaagaacta cgtccgtttg acggcgcgca agagtgcagg gactgctggc cgggctgggc
      901 accggggagc gtcggacatt tcggaagttg atgatccggg taaggagtaa tctcgaccca
      961 gggttcgaag cggagtagta cgccattcca gaaagcatat tttggagatg atgagattga
     1021 cattcggaga ccgaagtttg aggatcgcaa gtagaggcgg cacgttgcgg gtgggctcga
     1081 ctaacctcgg ctcgcccagg cgggcgcttt ctgcgtccga ggatgccgag gcttgagcgc
     1141 cgaggaagta gccacagaga aggtcgaggt ctcgaggcgg ctcctgatag gggttttgat
     1201 ctcggccgtc ttcgtctctg tactcaacag ctccatggtc aacgtcgtgg tccccgttat
     1261 aggaagggac tacggcgtca cggaggcgca ggtcgggtgg gtgatcaccg gctacttgct
     1321 gacctacgcc gtgggcatcc cgctctacgg gcgcgccgcg gacgtattca gcatccgcag
     1381 cgctttcatg gtaggtctca tcgcgttcgc tgccgggagc cttatctgtg cgtgggcgcc
     1441 gagcctcggt gcgctcgtct tcgggcggat cgtgcagggt gctggcgggg ctgcgatacc
     1501 ggcgctcgcc agcgtctccg tcacgaaggt actccctcct ggcgagcggg gggcggcgct
     1561 aggcctcatc gtctccagcg tgggcatcgg ggctgcaata ggccccattc tcggcggcgc
     1621 tgttggtgcg ctggctggct ggcactattt gttcgtgggg accctcattt tgacggccgc
     1681 cctgattcct gccgcgctct acgttctacc tggcgacagc cctgaggatg cgcgcggctt
     1741 cgacctgcct ggcggtttgt tgctcgggct cgcggctgga ttgttcctgt tcgggatcac
     1801 ccgcgggcag gtctccgggt tcggctcggc gggctcctgg gggtgcttcg ccggctcggc
     1861 cctggcggct gccggtttcg gctggcgcat aaggaacgcc gccgagcctt tcgtctcccc
     1921 tgatctcttc cagaacaggg agtacgtagc cgccgttgtc gtcgggtact tctgcatgct
     1981 caccaacgtg tcggcgctcg tcttcgtgcc gctgctcgtc tccgaggcca acgggctctc
     2041 gccggggcag gccgggctag tgctcacgcc aggcgcggtg gccctcgcaa tcatctcgcc
     2101 gctcgcggga cggtggtcgg acagcatcgg ggttcgccca ctcatattcg ccgggctggt
     2161 gaccatgctg ctctctgtgc tcactatctc cactttcggt gccggggctt cgcccgtggt
     2221 cgtctcgctc ggcatgctcg gagttggtgc cgggttcgcc ctcgccaact cccctaacgt
     2281 caacgccgcc gcctcctccc ttggacccga cgagctgggc gccggacttg ggatcttcaa
     2341 cggcgcgttc ttcctcggcg gcgggacggg gccggccgtg atcggggcgt tcctcgcggc
     2401 ccgcaaggag gcggaggccg gtgcgctgaa cccgctctac gggctcgaca ccgccgcgtt
     2461 ctctgacgct ttcctagtgc tcgccctgac gcttctcatc tctttggtgg cggcgctggg
     2521 gctgaggaac acgacagcaa aggagaagac gtgagcgact atggcaggga actcgagttt
     2581 ggtgtgagcg tggcaccact cgcagacccg cctgacttgg ctgcgcgcgt cgccaaggcc
     2641 gctgacagtg ccggtctgga gctggtgggt attcaggacc acccctacca gcgacgcttt
     2701 ctggacacct ggacgctcat ctcgacgctc gtgcctgtga ccgaacacat ccacttcttc
     2761 cccgacgtcg tcaacctccc gctccgtcct ccggccatgc tcgcgaaggc ggcggccagc
     2821 ctcgacgtgc tctcggcagg gagggtggag atcgggcttg gcgcaggggc tttctgggac
     2881 gccgtggccg gcatgggcgg cccgcgaagg agccccggcg aggccgtccg ctccgtcgaa
     2941 gaagcaataa aggttatgcg actggtttgg agcgacgagc gttcgctgcg cttcgacggc
     3001 gagttctatt ccttgaaggg catgcggccg ggtccgcacc cggtccatga catagggatc
     3061 tgggtcgggg cctacgggcc caggatgctc gacctcatag gaaggctcgc ggacggctgg
     3121 gtgccctcct tcggccacgc gcctccgggg cggctccctg agatgaaccg taggatcgac
     3181 gaaggcgccg aaagagcagg aaggcagcca cgggacatcc gtcgggtgta caacgtctcg
     3241 ggcagcatcg gcgccgtaga cgaaggttcc ctccaaggcc caccatcgag gtgggtcgag
     3301 acgctcacgg ggttcgccct ggaacacggt atggatactt tcatcttctg gccctccggg
     3361 gaccacgagc ggcaggtcgc ggtcttcgct ggcgaggtcg tgcccgcggt aagggaggcc
     3421 gtacggtcgg ggcgctcctg aggtaggatg atagacgacc accaggcgac gttctcgagg
     3481 gagacatgct ggataagaag gcgtacctca aagcagcccg gcatcatgcc cgtagggccg
     3541 gagtcagcct gctcagtgcg cggagcccac acagtatcat gagtttcctg agcgtggggt
     3601 gcatactcag cgcgggctcc attctgagca tcgggagtgt gggatctgtg ttgagcatcg
     3661 gcagctctgg cagcatcctc agcataggca gcacgggttg gatcctgagc atcgggagcg
     3721 tgggctccat cctgggccta accagcgcag gatacagccc ttcccgagaa cgtgaggacg
     3781 aggaagagtc gtaggtttgc aaagagaacc aaacctctct gcaagcattt cagcacggcg
     3841 gctgcgccgc cttgtcagca agtcagccag gaccttccaa tgagaaagct gacaagttga
     3901 aatgctgaca agtcgcctga aacaggcggc gtgctgacag gctgaaatga ttggatcgtt
     3961 cgaagttcct ctgctaggct gcacagtaag caacacggcc tacatagaga aggagggcgt
     4021 ccatgagcga gggtattctg cagcggttat ccaggggcat cgtcctcggt gacggcggtt
     4081 acttgctaga gttggagcgc cggggctacg tgcaggccgg tccgttcgtg cccgaaccca
     4141 gcattacgaa ccccgaggcc ctggaggagc tacacaggga gttcgtcgat gctggcgtcg
     4201 acgtgctcca gacgctcact ttctatgcca gcgaggacaa gctggcgacg gtcgggctcg
     4261 agggcagggt cgacgacatg aaccgcgagg ccaccaggat cgcacgcagg atcgcaggtg
     4321 aggccgaaag agacgtgctc gtcgcaggca acctgagctg cgcctgggtc taccggcccg
     4381 aagacccgga gacccaccgc cgcacccgcg agctcttcga ccgccagctc gacgtgatga
     4441 ccgcggaggg cgtggacttc gtgatagcgg agaccttcaa ctggctaggc gaggccatga
     4501 tagccgccga agccatcagg gccacagggc tccccgcgat ggtgaccatg ggccctgaca
     4561 acgatcccaa ctccttcgag ggctattcgc cgggcgagca ggtaaagcgt ctggccgacg
     4621 caggagtgga catcgtaggc gccaactgct ttcgctcacc agagtacatg ctcccgatca
     4681 tggaggagat gcgcgcagcc gtccccgacg cggttcacat ggcggcccag cccgtcgcgt
     4741 tccacgggac agacgagcgg cccaactacg tctcgctccc cgagttcccg tacgagttgg
     4801 agaagattac cctcccccgc gccacgatgg gagagttcgc actaagggcg cgtgacctcg
     4861 gatacaacta cataggctcc tgctgcggct cggtcgcggc ccacgtcagg gagatggcaa
     4921 gggccctcgg caagttatcc tccgacgaac gcgagtggcg tacgaggacg ggcatagccc
     4981 agtccggcta cgagctgcac cgtatgagag agcagtagga ccgcgaaagg cggcgtcgag
     5041 ctctctgaga aggcccacat tggggccagg gcgagaggtt ggcatgcaag gaacaggaaa
     5101 gaaggcgttc gagtacatcg tcgtgggttg cggcggcatc gggagcgccg ccgtgtactg
     5161 gctttcccgc gcggccggct ctgaggtcct cggcctcgaa cagttccatc tcggccacga
     5221 caggggcggc tcggaagacc actcgcgaat catccgcctc tcctaccacg accaggccta
     5281 caccacccta acgccccata cctaccaggc ctggtccgag atcgaggacg agtccggtat
     5341 acgactggtc ttcgaaacgg gcggcctcga cctggaatcc gtggaggacg cacccgatta
     5401 catcaaccac tatgccggtg cgatggcagc ggtaggcatc ccttaccagg aactctccgc
     5461 acaggaagtg atggatcgtt tcccgcaatt catcctcgat gagagcgtgc tcggcgtcta
     5521 ccaaccagga gccggcttcg tggacgcggg cagggccaac gccgtacaca tagccctggc
     5581 aaggaccagg ggcgcgacgg tcctcgacga ggcgccggtg tacggtgtgc gtacggccgg
     5641 tgagggcgtc gaggtcgaga cggctgcagg gaccttctcc gccagaaagc tcgtcgtcgc
     5701 ggcaggagcc tggaccaaca gggtgctcgg tcatctcggg tacgggatcc ccattacatg
     5761 cacccaggag caggtgacgt actttcggac gccccacttg tgggagttcg cgcctgacag
     5821 attcccggtc tggatctggc acggcggccc tggttacacg ttctacggtc tccccgtcta
     5881 cggtgctgct ggctccaaag ccggccagga taccggcggc gacgtcgtga gtgtcgacac
     5941 ccgcacgttc gacccgaatc cacgcatact cgccacactg cgcgactttt tgcagcgcta
     6001 catcccccgc tcgctgggtc ccgaactcta taccaagacc tgtctctacg acatgccgcc
     6061 agaccgcgag ttcgtgctag gcaccgtgcc agggataccc caggtcgcct tcttctgcgg
     6121 cgccggacac gccttcaagt tcgccggcct cgtcggcagg atcttgagtg agatagcgtt
     6181 ggaaggccgc acgcgctacg agatagatgc cttccgtccc gagcgtgagg cgctcacgga
     6241 cccggactac agaccgaccc tggcgatgta gcgtccggtg acggaattct acgtagagaa
     6301 cctcgacccg gcctctgtcg tcgctgtggc gaggcaaagg gcgcgggtgg cgatgggcga
     6361 ggaggcgcgt gagagagtga gcgcttcccg cgggcgcatc gagatcatgc tcgaaggcga
     6421 ccgtccggtc tacggggtga cgacaggctt cggggcgctc gccaacacca ggatcaccgc
     6481 agagaaacgc ctcgagctcc agcacacgct cctccgcagc cacgccgccg gtatgggacc
     6541 gttcatcgag cctgaggtgg tgcgggccat gatggcgatc agggcgaaga ccctatccgc
     6601 gggctactcc ggtgtccgcc ccgagctcat cgacgccctg gtggcgttcc tcaacgcgga
     6661 cctcaccccc gccgtgcccg agtatggctc tatgggcgcg agcggagatc tcgcccccct
     6721 cgcccacgtg gggctctgcc tcgttggaga aggccggctg ctggaggggg gagaaccagt
     6781 caagaccagc gaagctctag agcgcgccgg catccgaccc ttagggctcg ccacaaaaga
     6841 aggtctcgcc ctgatcaacg gcacagacgg tatgctggct atgctgctgc tctcgctcga
     6901 agacctgagg gtcctcctga agaccgccga tgtgaccgcg gcgatgtccg tcgaggccat
     6961 gctcggcacc gacagggtct tcgcggaaga cctccactcc ctgcggtctc atccaggaca
     7021 actcgcaagc gccggcaaca tctcgaaact gctctcaggg agtcccatcg tagcatcgca
     7081 ccgcgactcg ccccacctgg tccaggacgc ctattccctg cgctgcgccc cccaggtctt
     7141 cggcgcggcg cgcgacacgc tagatcacgc ctcactcgta gccggccgcg agctggcctc
     7201 agtcacggac aaccctgtcg tgctgccgaa cggacgggtc gagagcgggg gcaacttcca
     7261 cggcgagccg ctggccctcg tcctcgactt cctggccatc gcagcctcgg aggtcggtgc
     7321 catcacggag cgcaggatcg accgcatgct cgacccgaga cgttcggatg gcctgcccgc
     7381 ctttctcagc ccgcgacccg gggtcaactc ggggttcatg atgccacact acacagccgc
     7441 ggccctcgcg gaagagaatc gccgactcgc gaacccggcg agcacaggct ccctcccgac
     7501 ctcggcaatg caagaagacc acaactcgat gggatggagc gccggccgca agcttcgcag
     7561 gctcctcgat aacgtctcag gcatcctcgc cgtcgaagcg gcctgcgcgg cccaggctct
     7621 cgacctccgc taccccctgc agccaggcca cgcaaccagc gccatccgcc agcgtatcag
     7681 gcgagacatt ccgttcatcg agggcgacac ctttatggct ccgcacctgg agactgccaa
     7741 gagcctcgta acctctgggg agctggcact gacagccgaa cgagccatcg gccccctcgc
     7801 ctagagagcg gtcgacaggg aggctgatgg gataccataa gaacggatgg caacagagaa
     7861 ccatcaaggc aggatcgccc gtggccgcgg tgagccggcg accgtgatgg gcgcgtaggg
     7921 gatatcttct ggccggcaag gagatacctt ccggagcagg ggcactacgc agggcgctgg
     7981 cgtacctccg ctcgtacgcg tgggagagtg gggtcgcggt cctgtcgctc ttgctggttt
     8041 caggggcgaa cctggcgacg cctctactca tcgggcgcgc catcgacgaa ggcatcggtc
     8101 cgcggcggct cgaggtcatc ttcgttgtgg tcgggtggct ggtcgggctc gcgctggcac
     8161 gggccctgtt cacgttcttg caggggtatc tggccgagag ggcttcacag ggtgtcgcct
     8221 acgacttgcg ggatgctctg ttcgagagga tagagcgcct ctccttctcc tactacgata
     8281 gggtccagac gggtcagctc gttacccgcc tcaccagcga cgtggagcag atccgcacct
     8341 tcgccggctc cggcgtcgtc caactagcca acgccttcgt catgctcatt ggtacgacgg
     8401 tcttgctcct ctatctcgac tggcaactcg ccctcgttgc gctggccatc gttcccatca
     8461 tcgccgtgtt gctggttcgc tttgtcggta ggatcaggcc tcttttcagg gaggttcagc
     8521 agacgctcgg aaggctcaac accgtgctgc aggaggatct tctgggggtg cgtgtgatcc
     8581 gggccttcgc ccgtgaggac tacgagacgg cccgctacac atcggtcaac gaggagctgc
     8641 tgcagaagaa cctcaccacc gtgcgggtgt tctccaacaa cttccctttc gtctttctat
     8701 tcgccaacct ggggacgctg gcgatcatct ggtttggggg ctggcaggtt atcggcggca
     8761 ggctctcgat cggggatctc gtcgccttca acaccctgct cggctttatg ctcttcccga
     8821 tcctcacgat aggtttcctc tcggccagct tctcgcgcgc aggagcctca tcccagaggg
     8881 tcttcgatgt cctcgacgcc cccctcgacg taaaggacgc ccccgacgcc tccatcctcc
     8941 tccccctctc ctgcagggtc gacttcgacg acgtttcgtt ccgctaccct ggcagcgcgc
     9001 gcgacatcct ggccggcgtg tcctttaccg ccaggccggg gcagaccgtg gccgttctgg
     9061 ggactacggg ctcgggtaag tccacgctcg tcaacctcat accgcgcttc tacgacgtga
     9121 cgggaggggc cgtcaggctc gacggcaacg acgtgcgcga agtgacgcta tcctcgctca
     9181 ggtcccagat cgggatagtc ctgcaggaaa cgcgcctctt ctcaggcaca gtgcgcgata
     9241 acgtggcgtt cggcaaaccc gaagccaccg atgaggagat cgtggctgca gccgaggcgg
     9301 cccaggccgc tgagttcgta aaggagctct cggatggcta cgacaccgtt atcggcgagc
     9361 ggggcatcgg tctctcgggc ggacagcggc aacggatcgc catagcgcgg gcgctcctca
     9421 tcgatccccg gctcctcatt ctcgatgaca gcacctcggc cgtggacgcc gagacggagg
     9481 ctgcgatcca ggagactctg gacaggctca tgcgcgagaa gcaccgcacg gtcttcgtca
     9541 tcgcccagag ggtctcgacg gtgcgggacg ccgacctgat cctggtcctc gacaaaggca
     9601 gtatcgtagc cagcggtacc catgaggatc ttcggcggga gagcgaactc tacaacgaga
     9661 tcctcggctc ccaattgctc tccccctcgg agactgcagc atgaacagat tgacgggaac
     9721 atgatccagc gtctgctccg atcggagaac ggagtcggca acgtcgccgc gaacactgca
     9781 gagaggagag aaccatgggc gggaccacag gcggacagaa cgcaagagat tgtctctcct
     9841 tactgcgcac cctgcgcgct gtccgctcgt tcagaccaga ccacgtaccg caggaggtcg
     9901 tggacgacgt cctggaggtg gcccgctggt cagggagcgc gtccaacaaa cagccctggg
     9961 agatcctggt agtccgcgag cgggacacgt tgcgctccct cgcctcggtc gggggctacg
    10021 catggcacct ggcaggtgcg ccgctcggga tcgtcctcgt catggccggt gagcgggccg
    10081 agcaggagac ctacgacgag gggaggctcg ccgagcggat catgctcgcc gcccacgcac
    10141 acggggtcgg ctcgagcata ggttggttcg tcggctccgg cagggacgca gccagggagc
    10201 tactgggcat accaaaggag aaggtggtgc ggaccgcgat ctccctcggt taccccgagg
    10261 aggaggcccg cagatcccgc gcccgccgcg ggccggcgcg taaatccctc ggcgagatcg
    10321 tccacgagga aagctacgct taggagaacc atgaacgata ctttcgacgc cgaaaccctc
    10381 aggctcctcg acgagaccag agaagtgcgc atcgagaccc gccgtgaaaa tgcctccccc
    10441 gagcaccgca cgatcatctg ggtcgtggtc gtggaaggcg aggtcttcgt gcgctcgctg
    10501 cgcggcccca gagggcgctg gtaccgtgag atctcatcca accctgaggg cgcactgcat
    10561 gtgcacgacg accgcctccc cgtgcatgca gcacacgcca ccgaaggggc gattgtggat
    10621 gcggtaagcg ccgccttcag gagcaaatac cagcaatcct cgccagcctc tacagaggcg
    10681 atggtgcgtc ccgagacgtt gcccaccaca ctgaagctct cgcccgccta gaggctagtt
    10741 cgcgcgccag aaagagctgt ccgaggagga gagatccggg gcatagaatc cattgcacgc
    10801 gtgccgcagg agcgcgccag cgaccggggc gccaccgcca tgcgccttct aggagagctg
    10861 cgtccctatg taggcaagct ggtgctcgta ctcggtctcg tcgtgctcgg ggcggcggcg
    10921 caggctggcg gaccctggtt gatcggacgc tccatagacc gctacatcct gcccggcgac
    10981 gtagccgggc tctccaggat aatggtggtg ctcttcgggg tctacgctgt gggggccatt
    11041 gcctcgcggg gccaggtata ccaggtcggt tccatcgggc agagtatcct ggcctccatg
    11101 cgggccagga tcttcgaccg gctccagcac ctcccactgg ggttcttcga caggaggccc
    11161 gtcggagacc tgatgagccg ggtaaccaac gacgtcgaca ccctcaacca gctcttctcg
    11221 caggggctca cccagttact ggggtctctg ttcagcctca tcgggatcgt tattgcgatg
    11281 ctcttcctcg actggaacct cgccctcgcg tgcttcgcga tcatccctat catgctcctc
    11341 ttgacctcct ttttcgcccg ccgcgcgaga cgcgcctttc gcttgacgcg cgagacggtc
    11401 ggggacgtga ctgcgggagt tcaagaggac atcgtcgggg tgcgcgaggc gcaggccttc
    11461 aacaggaccg atgagaacat cgctcgtttc aggcggcgaa acgccgccaa ccgcgacgcc
    11521 aacgtgcaag ctgtggcgat cacctcggcc ttcgccccta caatagattt gctctcgacc
    11581 ctggccaccg ccctggtcat tggctacggc ggctatctag tcttcgccgg ctcgctctcg
    11641 atcggcatcc tgaccgcgtt cctgatctac gtccagcagt tcttccgacc gatacagctc
    11701 gcctcccagg tctacaccca ggcccaggcc gcggtcgcag gcgcagaacg catctacaac
    11761 atcctcgacg aagagcctga gcctgaggac ctgcaggacg cgccgaagct cggtgccgcg
    11821 agcggccgca tcaccttcga gcacgtgacc ttcgcatacg accctggcaa ccccgtcctg
    11881 cacgacgtgg acttccgggt cgagcccggt atgacggtcg ccctcgttgg cccgacgggg
    11941 gctggcaaga cgaccatagc gagcctgata ccgcgcttct acgacgccac acaagggagg
    12001 gtcctaatcg acggtaggga cgtgagggag ctcgcccgca aggacctcag ggccaacatc
    12061 gggatcgtgc tccaggagcc cttcctgttc tcgggcacca ttgcggagaa catcggctac
    12121 ggccgttccg gcgacccctc ggaggcgacg cgcgaggagg tggaggccgc ggccagcgtg
    12181 gtcaacgccg acgacttcat cacgtccctt cccgagggct acgatacggt tctgggtgag
    12241 gggggtgggg cattgagcca gggccaacgc cagttactct ctttcgccag ggcgatcctg
    12301 acagacccgc gcatcctcat cctggacgag gcgactagca acatagacac gcgcacggag
    12361 gcaaccatcc agagagccct ggccaagttg ctcgagggta ggacgagcat cgtcatcgcc
    12421 caccgtctga gcaccatcag aaacgccgac ctcatcctcg tcatagagag agggagcatc
    12481 gcggagcggg gcagccatgc cgcgctgata gagaagaacg gcctgtatgc ggacctctac
    12541 cgccgccagt tccgcgagcc cgtcccgtcc ggcggaagat cctgacccag cctgaaaggg
    12601 cggctggcgg tatcattcgt gctggcgtta gataatcggg agatgagagg agtttcgtat
    12661 gagcgtgctt ccggagagaa tactgctccc gacagacggc tcgaaggatg ctgtcagggc
    12721 agcagaggcg gcctcggatc tggccaagag gtgtggggcc gaacttcatg tcgttcacgt
    12781 ctggcacgac gtgcgggggt tcgcccacga cttcgtcaaa agggagctga ggcgccaggg
    12841 ccaggagata ctagacgagc aggtgaagaa gatcagggcc tccggtgtgg aagtcacgaa
    12901 ggcgtatctc aggaggggac gcacctccaa cgaggtcatc gccctcagca aagagatcga
    12961 cgctgatctc ctggtcgtcg gcagccgcgg gctaggcacg gtgcggcgca tcctgatggg
    13021 cagccaatcc gaggagatcg tgcaccacgc ccaggtcccc gtgctcgtac ttacgggcgc
    13081 agaagatttc tgggctccag cccggatcat catcggcgag gacttctccg acgacgcgcg
    13141 cagggccgga gagctcgccg ccggcatagg caagctctat ggtgccgagg gactgctcgt
    13201 ctactctcat cccgatctac cggaggttcc tcctggggag gcgaggtccg cggtccagaa
    13261 cctcacccag atgcgcgagc gggacaagag catgctcgaa gagcgtgcca gtgagctgga
    13321 ggagattctc ggtaggaggc cggagatcag gatctcagga ggatacccgg ccaacgtgct
    13381 cctcgaagcc tcccaggtgg tgcaaccctc gctcgtagcc gtcggcagca ggggcctggc
    13441 cggactgatt cggaccaggc tagggagcgt atcgaccaag gtcgtgaccg ccgccagggg
    13501 acccgtcctg gtctatccgc acgtcgatta gttaacgggc atcaggatct gcgtaggata
    13561 gtcccgcttg ctctgcagcc tgatcagccg aggaccgtct caccattctc agccgaatcg
    13621 ccccaaggta tgccggtgat aagatgatcc tacgtccgat gaaacctcga agaggagcac
    13681 catgatgccg cagactaccg gaatccacca cgtgaccgcc atctcgggcg tgccgcagcg
    13741 caacgtggac ttctacgccg gcactctggg cctcaggctg gtcaagaaga ctgtcaactt
    13801 cgatgatccg gagacttacc acctgtacta cggcgacggc gcagggtgtc ctggaaccat
    13861 catgaccttc ttcccttggg cgcacgcgcc aggtggacgc ataggggccg gccagctcgt
    13921 cgtcacctct ttctccatcc caacctcctc actaggctac tggacagagc gcctggtaga
    13981 gaatggcgtc cgcttcgaga gaccccgcga ccgtttcggc gatacagtcc tttctttcga
    14041 ggacccagac cgtctgcgcg tagagctcgt ggccacggaa gacgggcgac caggatggtc
    14101 cggcggtcct gtccaggccg agcactccgt cagggggttc caccacgtca ccctcgccgt
    14161 cgaggcgccc gaccgtaccg ccgggctgat gacagactca cttggcttca ggagcctgga
    14221 tgaggttgag ggtaggatca gactcgcctc aggcgagggc ggtcctggat acctggtgga
    14281 cgtggtgaac gctgccggct tcccccacgg gagcatgggc gtaggcaccg tgcaccacgt
    14341 cgcgttccgg gtccccgacg aggagaccca actcgcgctg cgtgaggagg tagccgcact
    14401 cggctacagc gttacgcccg tgctcgaccg caactacttc cgttcgatct acttccgaga
    14461 gcctggcggg gtcctgttcg agatcgccac ggatcccccg ggcttcgcgg tagacgagga
    14521 ccccgaacac ctcggcgaag acctcaagtt gccgccctgg ctcgagaccc gccgcgacag
    14581 gctcgaagag gttctcccac cgctgcgcgt cccgatagga gagagcgcat gagcgccgat
    14641 ctcggtttcg tccaccgcta cctgcctggc gaagatgagt ccagcccgac gctcctcctg
    14701 ttgcacggaa cgggcgggaa tgaagaggat ttgatcccgc tcggacagga gctcgctcct
    14761 ggcgctgcct tcctctcccc ccgcggcaaa gtctcagagt acggcgcccc gcgcttcttc
    14821 cgccgcctcg cagaaggggt tttcgaccag gaggacctcc tgctgcgcac gcacgagctg
    14881 gccgagttcg tggaagccgc ttcagcagag tacggtttcg accgctcgaa gctcgtcgcc
    14941 gtaggctact ccaatggggc caacgttgcg gcgagcacga tcctgctgca ccccggtctg
    15001 ctaaaggccg cggtcctgtt cagggcaatg gttcctttcg agcccgatct gacgcccgac
    15061 ctctcgggga tgcccgtctt cctggctgca gggcgcatgg accgcatgat cccgccagat
    15121 aacacacaac gcctggcgga catcctggac gaagccggcg ccgacgtcga cctccgctgg
    15181 cgcgacacag gccatcccct gacctatgag gaagttggcg aggcgaagga gtggctctcg
    15241 gaagtcctac caaagttctc tagtgcccaa gaccgtggga agtaaccacc tacccgaacg
    15301 gccaggttgc cgaggatgct agaatttttc tggagcgtct ggaaggacat acggccgcga
    15361 tttgcgggat gaaacaactg attgaccatc gtatctgaag ggcgggcggt atggcgaagc
    15421 cggtgatact ggcggttgac gacgacccgc aggtgctgcg ggccgtggag cgcgatctca
    15481 ggcgccggta cgcgcgcgag tacaggatac ttcgggcgga ctcaggggaa tcggcgctcg
    15541 acacactggg caagctcaag cttagaggcg accccgtagc gctctttctg gtcgaccagc
    15601 ggatgccgaa gatgacgggc gtcgagttcc tcgagcaggc tatcgagcgt tatcccgaag
    15661 ccaagcgtgt cttgctcacg gcctactcgg ataccgaggc agctattcgc gcgatcaacg
    15721 acgtaggcct tgactactac ctgcagaaac cgtgggaccc gccggatgag aacctctacc
    15781 caaacctcga cgacatgctc ggcgactggc aagccgacta caggccacct ttcacgggtt
    15841 tgcgggtcgt cggggaccgc tggtcgcccg actcgcaccg cactcgggag ttcctggccc
    15901 tcaaccgcgt accttaccgg tggcttgaca tcgagggatc cgaagaggct cgagagatcc
    15961 tggcgcacgc cgattctgga gcgccgaagc tgccgctcgt cgtctacgag gacggctctt
    16021 atgaggaggc gccgcagaac agggagatag ccgagaagat cgggcttcag acccgcgccg
    16081 ggcagcagtt ctacgacctg gtgatcgtcg gcggtggacc atccggcctt gcggccgcgg
    16141 tctacggggc ttcagagggc ctgaagaccg tcctcataga gcgcgaggcc cctggcgggc
    16201 aggccggaat gagcagccgc atcgagaact atctgggctt ccctgccggg ctttcgggcg
    16261 gcgatctggc gcggagggcc gttgaccagg cgaagcggtt cgaggtcgag atcctgacac
    16321 cccaggaggt gagcgaggtg cgcgcggagg acccgtaccg catcgtgacc ctcgccgacg
    16381 ggacggagat ctcgtgccac gccctgatca tcacgacagg cgtctcctac cggaaattgg
    16441 atgtgccagg tgttgatcat ctcacggggc gcggcatcta ctacggcgca gcccagacgg
    16501 aagctctttc gtgcaagggc gaggacgtgt tcatcgtcgg gggggcgaac tcggccggcc
    16561 aggccgcgat gttcttctca aagcaggcgg gcagagtctt catcctctgc aggggcagcg
    16621 acctgaggaa gagcatgtcc gattacctgg tgcgccagat agaagacatg gacaatatcg
    16681 aggtgctggt cgacacagga gtggtctccg tcgagggtga ggatcacctg gaacggataa
    16741 ccgcgaagaa cacacggacg ggcgaaaccg agaccgcgac ggcccattcg ctcttcatct
    16801 ttatcggggc tgcgccgcag acggagtggc tcgatggcgt cgtagagcgc gacgaacgtg
    16861 gctttatcct ctcgggaccg gacctgatgc aagggggtaa gcgtcccaag ggctggcggc
    16921 cagataggga cccgttcctt ctggagacga gcgtgccagg catcttcgtc gctggtgacg
    16981 tgcgtcacgg ctcggtcaag cgctgcgcct cggccgtcgg cgagggcgca atcgccatcc
    17041 agttcgtcca ccagtacttg agggagatct gaggtgcgag aggcgtcaga cgagttcgtc
    17101 tcgcagcaac acggcaagct gatctcgctc ggcaccatgg ccgcaggcct ggcccacgaa
    17161 ctgaacaacc ccgccgcagc tataggccgc agcgccgccg aggcccgcga ggcgtttcgg
    17221 gaagcgtcgg agaaggccgc ggaactgggt actctgcccc tcactgaaga acaacactcc
    17281 ttcgtcgccg ggcttccgga agaggtagcg cggaggaccg cggcctcgct cgacccgctg
    17341 gaagtgagtg acctggagga tgagcttgcc atgtggctgg aggaccgcag cgtcgaggag
    17401 gggtgggaga tctcctccac cctcgccggc gccgggctgg aaacggcctg gctcgaaggg
    17461 ctcgcagata gggtgcccca agaagcgcta ggcggtgttc tcggctggct gagagcaacg
    17521 atggccggtg acgagttgct gcgggagatc gagggtgggt ctgcacgcat ctccgagctg
    17581 gtcggggcga tcaagacatt tacgcacatg gacaaggcgg ccaccagaaa ggtcgacgtg
    17641 cacgctgggt tgaacagcac tcttatcatg ctcggacaca agctgaagaa gggcgacgtc
    17701 gaggtcgtcc gcgactacga gaaagacctc ccccacgtct gcggccacgc cagcgagctg
    17761 aaccaggtgt ggacgaacct gctcgacaac gccatcgacg ccgtcgacgg ccagggtcgc
    17821 atcacggtca ggacagccac cgagaacggg agggttctgg tcgaggtctc cgacgacggc
    17881 ccgggcatcc cagacgacgt gcgggaacgg atcttcgagc ctttctacac cacgaaggat
    17941 gttggcgaag gcacggggct cggcctggac atcacctacc gcgttatcgt cgaggaccac
    18001 aagggcgata tccgggtcct ctccgaaccc ggcgacaccc gcttccaggt gcgcctgccg
    18061 gtgaatgaac cgggtgacgc agagaaagcg gcggacgtat gatgcgcgaa gttcgggagg
    18121 agcaaggctt cactcccgag caggtcgagg cgcttatgcc ttacggcagg gagtcgaggc
    18181 tgcagacagg agacctcctc ttcgacgaga ggtccgtggt ggacagcttc tacgtcgtgc
    18241 tcgaggggga agtcgagatc acacgcctcg acggcgctgc ggagatcccg gttcagaccc
    18301 acgcacccgg ggagttcacc ggcgggctcg cggtgctgac ggggaggacc tcgatccaca
    18361 gggggcgggc aggcgcgccg agccgcgttc tggagatcga ctcggagaca ttcaggcgca
    18421 tgcccgccga ggtccctgac ctggccgaca tcttcatctc gggcctggcc aggcgcatgc
    18481 gctacacgca gcgggcctac cgccagcggg agaaactcgc ggccctgggc aagctctcgg
    18541 ccggcctagc ccacgagctc aacaatcccg cggccgcggc gcggcgggcc tccgaagagt
    18601 tgggcgaggc gatcctcgag gcccaactca cttccgtagg gcacgacgag cgcttctccg
    18661 ccggagaacg cgaggcgctg gtagccctgc aacgcgaaac ggcagcgggc aacgcagctc
    18721 ttctcgaccc gctctctcta ggcgacgccg aggatcggct cgccgattgg ttggaagagc
    18781 atgggtgtga ggagccttgg gatatggcgc cggcgttggc tgaggcgggc ctggacacgg
    18841 accgtctgga agcgatggcc ggggcattcg atgaccgctc gctcgcctgt ggtctggagt
    18901 ggctcgctgg gacgctggat ctcctcggcc tggcaagtga gatcgagact agcgctggac
    18961 gcatctccga gcttgtcggg gctatgaagg agtacaccta catggacaga gcggtggtcg
    19021 gagaggtcga tgtgatctcc ggcctccaga acaccctcac catcctcggc ccgaggctga
    19081 aggacgtcac tttgttgagg gagtatgaga aagacctgcc agggataccg gggcgcggcg
    19141 gcgagctgaa ccaggtatgg accaacctca tcgacaacgc catagatgct gtggacggcc
    19201 gcggaagcat caccatcagg gcgtacgtgg aaggcgaccg cgtggtggta gaggtcgttg
    19261 acgacgggcg tgggataccg cgcgaggcgc aggtccacgt cttcgagcct ttctacacga
    19321 ccaaagatat cggggccggg accggactcg ggctagatat cgtacggcgc atcgtcaccg
    19381 gtcatggagg cgagatcttt gtccagtcgg agcctgggga gacatgcttc acggtaaggt
    19441 tacctttaga tactacacag aacggaggtt gatctcgatg ggaaagacac acgtgtatga
    19501 ggagatctgc acccagaagc gcgaggtaga tgcacgggtg ctcgagcaga ccgtctccct
    19561 cgcggtcgag atcgcgcgcg agggccgcga ggggcggaag atagggacgc tcttcgtggt
    19621 gggcgactct ggcgaggtca taaagcgatc caagcccttg atcctcgacc ctttgcacgg
    19681 acaccctgac gagagcaagc agatagagga tcccgacatg cgggagacca tcaaggagct
    19741 ggcccagctc gacggtgcct tcctggtctc gaacgcgggc gtggtgctct ccggcgcccg
    19801 ctacatagac gccgcctcgg acagcctgga tctgccgctc gggttgggca gtcgtcacgt
    19861 ggcaggcgct tctatctccc gtcagacgag agccgtggcc gttgtcgtct cggagagctc
    19921 gagggtcagg atgttcgacg acggcgagct ggtctctgag atcgtacccg agctctggat
    19981 gatagacggc tacacgagcc gcatggaggg ccgcaccttc acccgccgcg acgaagacgt
    20041 aacggtaata ggccgtgccg agtaaggccg acgaagtact cgacttctgg ttcggccgcg
    20101 caagcgagcc tggctacggc gagtttcgcg acgagtggtt ccgcaaagac cccgagttcg
    20161 acgccagggt cacggaccag ttcgccggtc tctacgagga ggccgcagcc ggcgacctcg
    20221 acggctggcg cgacgatgca aggagctgcc tagcgctcgt aatcgtgctc gaccagttcc
    20281 cccgcaacat gttccgtggc gacggacgca ccc
//
LOCUS       DASTKM010000092        18422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_20334, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000092 DASTKM010000000
VERSION     DASTKM010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 18422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18422
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20334"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFQ10_03700"
     CDS             <1..437
                     /locus_tag="VFQ10_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9964476.1"
                     /translation="PEEATVCVGTEFSTRDDGLPGMEEHYDFEFPKENIVKIDEGLIY
                     QQTDKGKQCNFGEVFQTDGRIEALNLELIKDDKSFFPIYNPALNVSKEVIEKNPQITK
                     LFAPISEKLTTEELQKLNAKVDVQGQLEDQVAEEWLSQNGFI"
     gene            739..1581
                     /locus_tag="VFQ10_03705"
     CDS             739..1581
                     /locus_tag="VFQ10_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET9964477.1"
                     /translation="MRVALAQIDCVLGDTETNLSRAKGAVAEAKNRSADLTVFPELSL
                     SGYALGELGDDVPIEAHSKPIISLAETAGEMAVVVGFCEEGQRFQTYNSATYLEGGVL
                     VHLHRKLYLPNYRIYEERKHYNPGQNMRAFDTRFGRMAMLICNDAWQTFLPFIAVQDG
                     AQVLIIPAASGSYPYPELLDTRDYWRGITRFYARMLESYVIFVNRVGKQDNLLFWGYS
                     HVVDPWGTVVAEAPLHEEALITVDIDLGNVRRRRREVPLVKEARLALLARELNRLAEE
                     GGDL"
     gene            1622..4480
                     /locus_tag="VFQ10_03710"
     CDS             1622..4480
                     /locus_tag="VFQ10_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET9964478.1"
                     /translation="MPDIPEPSLRQLHAALDGKLEGYLRTDEASRAMWSTDASIYLRK
                     PVGVVVARSEEDVELALSAARDVGLSITPRGTGTSLAGQATAPGLALDVSEMRRALEV
                     DLEERRCVVEPGLVQGELNAIVGPHGLVFGADTSTSDVATLGGMVGNNSAGMRSLVYG
                     MTSDQVLGLRCVLASGETVDLVSLNREEARGRARHADTLARLLRGAMEIGDRYNDEIR
                     RRFPKMIRRVSGYGLDALVDPETLDLTRLICGSEGTLAVVTRAEFRLHPLPEARALAS
                     FEFDTLPAAARATGLFLQMNPSAIELLDDVAIGRARANPAYRDSTRFVKGNPKAVLVV
                     EWSGTKEELDERFTKLDELAGEAGALVATPLRTEAQMSQTVTLRKSTLPLLLGTTDKE
                     KPVAFVEDAAVPPDRLEEFVVRFEEIVEKNGTWACFYGHASVGCLHVRPALDTSDPDG
                     VSRMRRIAEEVADLVVDCGGSISGEHGDGLSRSEFMEKMYGGEILRAFADVKRLFDPE
                     GMMNPGVILAPPRMDRQLRIGPGRKRLPIQTNLSFDDQGGFAKAVELCNGSGFCRKRT
                     GGTMCPSYMVTLDEQDTTRARANMLRSVIEGTLPPEELTGRRMKEVMDLCVGCKACKT
                     ECPSRVDVASMKTEVLYQAGREHGFSLRQKAAGHIRRQLALAALVPSLYNAFAGTRLA
                     RRTAAMLGIDPRRSLPRVAKKTFTRRFPELPQGAGPAEVALFNDTWNEYQRPEVGEGA
                     VRLFAAAGARIHLPEVVCCGRPMLSEGLVDEARKNAGRNLDLLMPLVERDVPLVGLEP
                     SCILTLRDDYSKLLPDDERVEKLARATRLFEEAMLELDGELLLREGAPVLLHGHCHQK
                     ALVGTGPTERVLGLAADVEVVDSGCCGMAGLFGYEKGHYEVSMKMGERRLFPAVREAG
                     ERVVVAPGTSCREQILDGTERRALHPAEYLASLLDRST"
     gene            4487..4672
                     /locus_tag="VFQ10_03715"
     CDS             4487..4672
                     /locus_tag="VFQ10_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964479.1"
                     /translation="MAEREAALWFAFRDDRMLVLEDAPVRVPPVGSPDEISIARAMID
                     DFARRRGLDPEDPETPG"
     gene            4802..7021
                     /locus_tag="VFQ10_03720"
     CDS             4802..7021
                     /locus_tag="VFQ10_03720"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET9964480.1"
                     /translation="MAILGGAYTLWRRPARVPGAIAGVARDVSWGPHRIGEFTVVLET
                     GPSLSDVVLSVSHSSRPDRILWQSIPGASFLAAAEGEETVRESSGHFFIDDEIDRPHL
                     DQTIDYVEKHQSTLVVGGRLTRRGDSEGVDYTLTFLLVTEERLRFEVEVEEPYNRVYL
                     TYASSPEERFFGFGTQYTYFDMKGHKLPIFIQEQGIGRGKQPVTWAADWQADAGGSPY
                     TSYASVPHYITSQMRSLFLENYEYSSFDLREQDRVQVEVFSSRMTGQILSGETPVELI
                     ELYTEYSGRMRLLPEWILSGAVVGLQGGTDMVLDAYDELEALGTPVAALWLQDWVGQR
                     ETNFGMQLWWNWELDEDHYPGWDRLREQLERDDVRLITYIGPWLADDVKNEKKNHRRN
                     LFEEAERRGYLVKNREGEPYRVKTTDFSAAFVDLTNPEARDWIKDVIKEELIGNGASG
                     WMADFGEGLPYDAVLFSGADPKSYYNRYAEEWAELNREAIREAGRGEDIVFFNRSGYT
                     RSPRYSTLFWLGDQLVDWDEHDGIKSAVTGLLSSGLSGYSLQHSDIGGYTAIDNFLLK
                     YHRSKELLRRWIELGAFTVVFRTHEGNRPKVNHQIYSDRETLRHFDRFAKVYAAWKPY
                     RMELVKKASETGIPVVRHPFIHYPNDPEVLGLNYQYMVGPEFMVAPVLDPGSDSVEIY
                     LPAGKWVHLWTGERYGLPQRGAYVTIPAPIGEPAVYYKEGSAEGTRFREELERRGLL"
     gene            7068..7460
                     /locus_tag="VFQ10_03725"
     CDS             7068..7460
                     /locus_tag="VFQ10_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9964481.1"
                     /translation="MKRENVTSGTVWESRVGYSRAVRRGRFVYVSGTTATDGSGGYVG
                     VGDPYAQTVQAINNIKVALQAVGADLSDVVRTRIYVVNIEDWEKIGSAHGVCFGQVRP
                     ATSLIEVSRLIAPEILVEIEADALIDES"
     gene            complement(7482..8582)
                     /locus_tag="VFQ10_03730"
     CDS             complement(7482..8582)
                     /locus_tag="VFQ10_03730"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HET9964482.1"
                     /translation="MFSRVGAGLSAGYDRLRTSVWSILQAAVASSLAYFVAEVFVGHE
                     QPFFAPIAAVICLSVTLGQRNRRAIELVFGVAVGLMVADLLVLVIGNGTLQIAVVVLL
                     AMAAAVFFGGGTILVNQAAVSAVLVVVLQPPEDLFSPERFVDALIGGGVALAINYLFP
                     INPERLVERAAAPVFEELVAVLEEISAVLADGDERRAERVLERARELDGRVRTLNEAL
                     EAGHETARLSPTRRRSLRQLEFYGSAGIRIELAVINTRVLARGVYNAIRRGDRIPPKL
                     SEAVLDLSRAVRALAAFLEDGEGPEEARRWALEAARTATEILEERHELGISVLVGQVR
                     SAAVDLLRSTGMDQASALQALEDAAGRASEIG"
     gene            8733..9245
                     /locus_tag="VFQ10_03735"
     CDS             8733..9245
                     /locus_tag="VFQ10_03735"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET9964483.1"
                     /translation="MGRRRIEPGQGLESVWDYPRPPRVEDVDKKVKVVFGGVTLAYTT
                     RAKRVLETSHPPVYYIPRQDIQMEFLRGSGRTSHCEWKGRASYYDIATKDEITGEAKS
                     EKGVAWHYPDPVPEFEGLKDHVAFYPNRMDACWVGGEKVEAQEGDFYGGWITPDIVGP
                     FKGAPGTWGW"
     gene            9253..9888
                     /gene="pdxH"
                     /locus_tag="VFQ10_03740"
     CDS             9253..9888
                     /gene="pdxH"
                     /locus_tag="VFQ10_03740"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET9964484.1"
                     /translation="MDASSLRKEYTSAGLDKADVDPDPIVQFQEWFEKVIDADLHEPN
                     AMILATATADGKPSARTVLLKGYDERGFVFYTNYEGRKAVELESNPRAALVFYWGELE
                     RQVRVEGTVSRVSVEESDEYFASRPRGSRLGAWASEQSRVVDGRRTLEQKLHSLQAEY
                     ENREVPRPPFWGGYRIEPEAIEFWQGREDRLHDRLVYRREDGAWRIERLQP"
     gene            9960..10778
                     /locus_tag="VFQ10_03745"
     CDS             9960..10778
                     /locus_tag="VFQ10_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9964485.1"
                     /translation="MKLVTYSVGGGETSVGSLEGEEIRPLGHEDMIEFIEYGGSPEPG
                     EDTVPLGDARIHAPISRPQKIIGIGLNYEDHAAETGADIPEKPIVFAMYPNALIGPGE
                     AIRIPSITEQADYEAELTVIIGRAARNVSESEALDYVFGYTNANDVSSRDLQFSEGGQ
                     WTRSKSIDTFCPLGPFIVTTDEIEDPQDLSIRCILNGEVMQEGTTQKMIFSVAELVSF
                     LSQGMTLVPGDVILTGTPPGVGAARDPQVWLKAGDEVTIEIQGLGSLTNPVEKD"
     gene            10972..11370
                     /locus_tag="VFQ10_03750"
     CDS             10972..11370
                     /locus_tag="VFQ10_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964486.1"
                     /translation="MRVIGVLLILEVIGLLVLGGYEFLQVDWRGINLRSPQNQAVEVA
                     VFALFVPSAVLTLLSALSFLFLRRRGWLLAAIAQGLSLAVCLWLYSLFQPGYVYPIMA
                     YCILMILYLNSHDVRMVFRPGQSPQESHES"
     gene            11360..13228
                     /locus_tag="VFQ10_03755"
     CDS             11360..13228
                     /locus_tag="VFQ10_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964487.1"
                     /translation="MKAESPCVEAATALLRRFEPVIRSTTGDKFYPMDVEPYVRACSL
                     WVQSPGEEAVCIVPGGKLSLDRLAQQPMDEAGAVHFLKFTDAEDLGPDGRGRRLRLFR
                     RRAKDRKESTRAFRAGRGRLARVGYFSRFVDALYSIALLARGRVPGESAAAASVAYER
                     IMAEQEQYRYHGRVLRQDGWIVLQYWLFYPFNDWRSGFFGANDHEADWEKVFIYLSES
                     EDGEVSPEWVAYAAHNYTGDNLRRRWDDPELEKVGEHPVIYVGAGSHASYYAPGEYLT
                     ELTLPLPSPLARVSGAIRTFWKTRLGQYVGDGEEDGGPGYFSIPFVDYARGDGLSVGE
                     GGDRSWDEPRLISDPPPDWVSGYRGLWGLYARDPFEGEDAPAGPMYNRDKSINRAWYD
                     PVGWAGLDKVPPPTETLEAVLEQRADIISRCDVLRNEIDDKSRQLKKLGAELAAMCDR
                     SHLDAPYQEGTKRVAELSGELDRLRGKLATDEAVSESLAEYAGRLKAGERDPARSHIS
                     RAHRPASEDELRVSRVAEAWAAISVSLMLISFVGIAMFEREHLISMLVFSIAFFAFVE
                     AGFRGRLVNLVSSANIGLAVVATLIIVYEFFWQLVVAAVLVIGIYVLWDNLRELRR"
     gene            13266..13664
                     /locus_tag="VFQ10_03760"
     CDS             13266..13664
                     /locus_tag="VFQ10_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET9964488.1"
                     /translation="MARTIRVVVAKVGLDGHDRGAKIIARALRDAGMEVIYTGLHQTP
                     EQVVEAAIQEDVDAIGISILSGAHMTLIPRIIELLEENEAEDILVFVGGTIPKEDITR
                     LKELGVGEVFTPGTPTKKAVEYVRQATPAS"
     gene            13755..14528
                     /locus_tag="VFQ10_03765"
     CDS             13755..14528
                     /locus_tag="VFQ10_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9964489.1"
                     /translation="MDYVSVQHDDDVAVLTIDRQEKLNALDMQVVEEIGQALLDLEAE
                     GPRAIVVTGAGERAFVAGADIRAMSIMEPLEAKRFSEIGHAAMALLDRSPVPTIAAVN
                     GYALGGGCEVALACDIRIAAENATFGFPEVSLGILPGMGGTQRLPRLIGPALAKELIF
                     TGRRISAEEAREIGLVNRVVAEGEALNAARELAAEISANGPLAVRHAKAAANRTLDVD
                     LVSGLEFEADQFALLFATEDAREGMNAFGEKRKPEFEGR"
     gene            14543..14923
                     /locus_tag="VFQ10_03770"
     CDS             14543..14923
                     /locus_tag="VFQ10_03770"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9964490.1"
                     /translation="MRVRYSEIDAQGHVNNATYLSYFEVGRVEWLRATGHSYREMEAR
                     GRGLVVVEALLHYRRPAFFDDELTLSTHLADIGKVSLRFDYEVLKDGEVLVTRHTRHA
                     CVDLATGKPIRMPEELLMLAKGTP"
     gene            complement(15178..15522)
                     /locus_tag="VFQ10_03775"
     CDS             complement(15178..15522)
                     /locus_tag="VFQ10_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964491.1"
                     /translation="MRGGAYKVEADLSGLKLASFVLTRGLSDVDLTLPEPSGVVPVRL
                     TGGASEVNIRRPAGVEARLSMIGGVSKLTFDEQSFDAVGGKLRLRSPGYDSVSDRYEI
                     EVSGGASKVTVQ"
     gene            complement(15555..15836)
                     /locus_tag="VFQ10_03780"
     CDS             complement(15555..15836)
                     /locus_tag="VFQ10_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964492.1"
                     /translation="MTREETARIRAASKGGEGGEFSAPLGSAQSGRMMFANGVSRLTL
                     RAASGMDDLYRARFEGPVPKVEVADGTVTFRYPRRFSGLFEWRSRSGGR"
     gene            15893..16771
                     /locus_tag="VFQ10_03785"
     CDS             15893..16771
                     /locus_tag="VFQ10_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9964493.1"
                     /translation="MFGFLGPNGAGKTTTIRMLATLLRPHSGTARVFGHDVVGESDAV
                     RDRVSLTGQFASVDEDLTGLENLVLIARLLGYSRKGAKGRAGELLHAFGLSEAAGRLA
                     KTYSGGMRRRLDIAASIVVTPDLLFLDEPTTGLDPRNRNQVWDIVRAMSSGGTTVLLT
                     TQYLDEADRLADRLAVIDNGKVIAEGTPGELKNSVGSDVLRVRLRESEARPRAERVLS
                     RALGVAVETPSDPVALLARVSEPERAPDALAELPRAGIAVTDFSLGQPSLDEVFLALT
                     GRPAKQETYKEEEEAV"
     gene            16768..17607
                     /locus_tag="VFQ10_03790"
     CDS             16768..17607
                     /locus_tag="VFQ10_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9964494.1"
                     /translation="MTDPAGVSRTTIAEEALRSALSSGERPKRPNALSASLTFGWRAL
                     LKIKHVPEQLFDAVGNPIIFTLLFTYLLGGAIAGSTGAYLQFLIPGILVQTVILITAY
                     TGFNLNTDISKGVSDRFRSLPIWRPSVIVGALLGDVARYALASAIVLTLGLVLGFRPE
                     GVVGVLLSVALLVVFAFSVSWIWTALGLVLRTPNSVLWVSTLVMFPLTFASNIFVEPK
                     TMPGWLETAVGYNPITHLVSAIRGLMSGTMPAGHISWLLVGCAMLVAVFAPITVRLYR
                     NKE"
     gene            17686..>18422
                     /locus_tag="VFQ10_03795"
     CDS             17686..>18422
                     /locus_tag="VFQ10_03795"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HET9964495.1"
                     /translation="MNPLLGQHAYRLSEAGVAGNRTSHGAENNLRKIRLLLEHDASNT
                     FGMEDLLQDVGFDEAYDAVAHQIGNPPDREENPERGCIDPARTAAGLVEAGGRIEAVA
                     GSGGRLIFATGHPGAMILYYLGLARWARELGGDALTAETRGRYERGVSLDWAESVAAL
                     GDGASLFHTHDPEPMTDVLRQTGAVDLVVADHGFAGAAIATGVPTIVVMDTNDPAFAV
                     VAGRGADVTVVPMDDNRPLNAYSTALDV"
BASE COUNT         3569 a         5780 c         5889 g         3184 t
ORIGIN      
        1 acccggaaga agccaccgtg tgcgtgggaa cagagttctc gacgcgcgat gacggcctgc
       61 cgggtatgga ggaacactac gatttcgagt tcccgaagga gaacatcgtc aagatagacg
      121 aaggtctgat ctaccaacaa accgacaagg gtaagcagtg caacttcggt gaggtgttcc
      181 agaccgacgg tcgcatcgag gcgctcaacc tcgagctcat caaagacgac aagagcttct
      241 tcccgatcta caacccggcg ctcaacgtga gtaaggaagt gatcgagaag aacccgcaga
      301 ttaccaagct gttcgcgccc atctcggaga agctgactac agaggagctg caaaagctga
      361 acgcgaaggt cgacgtgcaa gggcagctcg aagaccaggt agccgaagag tggctaagtc
      421 agaacggctt tatctagcaa acggcccgac ggcgattgtc ggatggtaag cccggcaatc
      481 gcttctttcc ttgatgtcct cggccagagg actcctttgc ctgaccgctc cacggtcggc
      541 tggcccgcgt ctcttcttgc gggccgatca gagatcgtag ggccttaacc ggcgtgtggc
      601 ataaagtacg atccgggcga gttatggaac ccggatcgcc ttgtttcccg cccacccatc
      661 gttccgttct cctagtggtt ctattccggt accacgacgt gcggggggag cgcatcatcg
      721 agaggtatag cacttaacgt gcgcgtagcc ctggcccaga tcgactgtgt cctcggcgac
      781 accgaaacga acctcagcag ggccaaaggg gcggtggcgg aggcgaagaa caggagcgcg
      841 gacctcaccg tcttccctga gctgagtctg tccggctatg ctctcggaga gctcggtgac
      901 gatgtcccca tcgaggcgca cagcaagcca ataatctccc tggccgagac cgcgggagag
      961 atggccgtgg tcgtggggtt ttgcgaggaa gggcagcgct tccagaccta caacagcgca
     1021 acctatctcg aaggaggagt cctcgtgcac ctgcaccgca agctctactt gccgaactac
     1081 aggatctacg aggagagaaa gcactacaac ccgggacaga acatgcgcgc cttcgacacc
     1141 aggttcggcc gcatggccat gctgatctgc aacgacgcct ggcaaacgtt tcttcctttc
     1201 atcgcggttc aggacggcgc ccaggtgctc attatccccg ccgccagcgg ttcctaccct
     1261 tatcccgagt tattggacac aagggactac tggcgtggga tcacccgctt ctacgcccgc
     1321 atgctcgaga gctacgttat cttcgtcaat cgagtcggaa aacaggacaa cctgctcttc
     1381 tggggctact cgcacgtcgt ggatccctgg gggacggtcg tggcggaagc gccgctccac
     1441 gaggaggcct tgatcaccgt ggacatagac cttggcaacg tccgccgacg ccgcagggaa
     1501 gtgcccctgg tcaaggaggc ccgcctcgcc ctgctggcca gggaattgaa tcgtctcgcc
     1561 gaagaaggcg gggatctctg attggcgaca cgtccgctct gcatgtaaag ttgaccggac
     1621 catgccggat atacccgaac cctcccttag gcaactccac gccgccctcg acggcaagct
     1681 cgaagggtac ctgcgcaccg atgaagcctc acgcgccatg tggtccacgg atgcctccat
     1741 ctacctccgc aagcccgttg gtgtcgtcgt cgcccgctct gaagaggacg tggaacttgc
     1801 tctatcggcg gcccgcgacg tcgggctctc gatcacaccc cgtggcaccg gcacgtccct
     1861 ggccgggcag gcgacggccc ctgggctggc cctcgatgtc tccgagatgc gccgggcact
     1921 ggaggtagac ctcgaagagc ggcgttgcgt ggtcgagcca ggactggtgc agggcgagct
     1981 caacgcgatt gtcgggcccc acgggctcgt gttcggggcg gacacctcta cctccgacgt
     2041 ggcgacccta gggggtatgg tcgggaacaa ctcggcgggg atgcgctcgc tcgtatacgg
     2101 gatgacctcg gatcaggtcc tcggcctgcg ctgcgtgctg gcctccggag agacggtgga
     2161 cctagtttcc ctcaacaggg aagaagccag aggacgcgcc cgtcacgccg acacgctcgc
     2221 aaggctgctg cgcggggcga tggagatcgg ggaccgctac aacgacgaga taaggcgccg
     2281 gttcccgaag atgattcgtc gcgtctccgg ctacggtctc gacgccctcg tcgaccccga
     2341 gaccctggac ctcacgcgcc tgatatgtgg ctccgagggc acgctggccg tggtcacccg
     2401 cgcggagttc agactacacc cgctccccga ggcccgcgcc cttgcctcat tcgagttcga
     2461 caccctccct gcagcggccc gtgccacggg cctgttcctg cagatgaacc catcggcgat
     2521 agagctgctc gacgacgtcg cgataggccg cgcgagggca aaccccgcct acagggactc
     2581 gactcgcttt gtcaaaggca accccaaggc cgtcctggtc gttgagtgga gcgggacgaa
     2641 agaggagctg gacgaacgct tcacgaaact cgacgaactg gccggcgaag ccggggcgct
     2701 ggttgcgacg ccgcttcgca cagaggccca gatgagccag accgtcacgc tgcgcaagtc
     2761 caccctgccg ctcctgctcg gtactacaga caaagagaaa ccggtcgcct tcgtggagga
     2821 cgcggccgtg ccgccggacc ggctggagga gttcgtcgtc cgcttcgagg agatagtgga
     2881 aaagaatggt acctgggcct gcttctacgg acacgccagc gtgggatgcc tgcacgtcag
     2941 gcccgcgctc gacacctcgg accccgatgg cgtctcgcgg atgcggcgca tcgccgagga
     3001 ggttgcggat ctcgtcgtcg actgtggcgg gtccatctca ggggagcacg gcgatggcct
     3061 ctcgcgctcg gagttcatgg agaagatgta cggaggggag atcctgcgcg ccttcgcgga
     3121 cgtcaagcgt ctcttcgacc cagaagggat gatgaaccct ggtgtgatct tggccccacc
     3181 ccggatggac cggcagctgc gcatcggccc cggccgaaag cgcctcccca tacagacgaa
     3241 cctctccttc gatgatcagg gcggattcgc gaaagctgtc gaattgtgca acgggagcgg
     3301 cttctgccgc aagaggacgg ggggcacgat gtgcccctca tacatggtga ccctagacga
     3361 gcaggacact acgagggcgc gggccaacat gctcagatcc gtgatcgaag gcaccctccc
     3421 accggaggag cttacggggc ggcgcatgaa ggaggtcatg gacctctgcg tcggatgcaa
     3481 ggcgtgcaag acagaatgcc cctcccgggt agacgtcgcc tcgatgaaga ccgaggtcct
     3541 ctaccaggcc ggcagagaac acggcttttc cttgcgccag aaggcggcag ggcacatccg
     3601 ccgtcagctc gccctggccg ccctggtccc ttcgctctac aacgccttcg caggaacacg
     3661 cctcgcccgc cggaccgctg ccatgctagg catagacccc cgccgctcct tgccacgggt
     3721 ggcgaagaag actttcacca ggcgcttccc cgaactgccg cagggcgccg gacctgcgga
     3781 ggttgcgctc ttcaacgaca cctggaacga gtaccagagg cccgaggtcg gggagggagc
     3841 ggtaaggctc ttcgcggccg cgggagcgag aatccatctg cccgaggtcg tctgttgcgg
     3901 gaggccaatg ctgtctgagg ggctcgttga cgaggcacga aagaacgccg ggcgaaacct
     3961 ggatctcctt atgcccctcg tcgagcgcga tgtcccgctg gtcggcctcg agccgagctg
     4021 catcctgacc ctgcgcgacg attacagcaa gcttctcccc gatgacgagc gcgtcgaaaa
     4081 actggcgcga gcaacccggc tcttcgagga ggcgatgctc gagctcgacg gagagcttct
     4141 gctaagagaa ggcgccccgg tcctcctgca cgggcactgc caccagaagg cgctcgtagg
     4201 gacgggcccg acggagcggg tgcttggtct cgcggccgat gtcgaggtcg tggactccgg
     4261 ttgctgcggg atggccgggc tcttcggata cgagaaggga cactacgagg tctcgatgaa
     4321 gatgggagag aggagactct ttcccgcggt gcgtgaggcc ggggagcggg tcgtcgtcgc
     4381 gccgggaacc agctgcaggg agcagatcct tgacggaaca gaacggcgtg ccctacatcc
     4441 agccgagtat ctggcctcgc tcctcgacag gtcaacctaa agtccgttgg cggaacggga
     4501 ggcagcgctg tggttcgcgt tcagggatga caggatgctc gtgctcgaag acgcgcctgt
     4561 ccgcgtaccg cccgtgggta gtccggatga gataagcatc gctcgcgcca tgatagatga
     4621 cttcgctaga cgacgcggcc tcgaccccga agacccggag acaccagggt aggccgcgtc
     4681 gtatggctgg gcgtacctct cttgtgagat gcgagggtaa acagagccgg aagagatcgc
     4741 ggagaggaga ttgataacgc ggaggaagtt tatttggcta gccggtattg gtgtggctgg
     4801 gatggccata ctgggtggtg cctacacgct ctggagaagg ccggctcgtg tccccggcgc
     4861 tattgccgga gtggcgcgcg acgtctcctg gggaccgcac cggatcggcg agtttaccgt
     4921 cgtgctggag actggtccca gcctttcaga tgtagttctc agcgtttcgc acagctccag
     4981 gcctgacagg atactatggc agagcatccc cggggcgagc ttcctggcag ccgccgaggg
     5041 cgaggagacg gtcagagagt ccagtgggca cttctttatc gatgatgaga tagacagacc
     5101 acacttggat caaaccatcg actacgtcga gaagcaccag tctacgctgg tcgtcggtgg
     5161 acggctcaca cgacgaggcg attcagaagg cgtggactat acgctcacct tcttgctcgt
     5221 gaccgaggaa cggctgcgct tcgaggtcga ggtggaagag ccctacaacc gcgtctatct
     5281 cacctacgcc tcctcgcctg aggagcgctt cttcggtttc gggacgcagt acacgtactt
     5341 tgacatgaag ggccacaagt tgcccatctt catccaggag cagggtatag gcaggggcaa
     5401 gcagcccgta acctgggccg cggactggca ggccgacgcc ggcgggagtc cgtacaccag
     5461 ctacgccagc gtaccgcact acataacgag ccagatgcgc tccctgtttc ttgagaacta
     5521 cgagtactcc tccttcgact tgcgggagca ggacagggtc caggtcgagg tcttctcctc
     5581 ccggatgaca ggccagatcc tgagcggaga aaccccggtc gaactgatag agctctacac
     5641 agagtactcg ggcaggatgc gcctgttgcc agagtggatc ctcagcggcg cggtcgtcgg
     5701 cttgcagggc ggcacggata tggtcctcga tgcatacgac gagctcgaag ccctcggcac
     5761 acccgtcgct gcactctggc tgcaggactg ggtggggcag cgagagacca acttcggtat
     5821 gcagctgtgg tggaactggg agctcgacga agatcactat cccggctggg atcgactcag
     5881 ggagcagcta gagagggacg acgtgaggct cattacctac atcggcccgt ggctcgccga
     5941 cgacgtgaag aatgagaaga agaaccaccg ccgcaacctc ttcgaggagg cggaaagaag
     6001 aggttacttg gtaaagaatc gggagggcga accttacagg gtcaagacca ccgacttctc
     6061 ggcggccttc gtggacctga cgaacccgga ggcgcgggat tggatcaagg acgttatcaa
     6121 ggaggaactc atcggcaacg gggcctcggg atggatggcg gacttcggtg agggtctccc
     6181 ctacgacgcc gttctgttct ccggcgcgga cccgaagagc tactataacc gctacgccga
     6241 ggagtgggcc gagctcaacc gcgaagccat ccgggaggcg gggcgaggag aggacatcgt
     6301 cttcttcaac cgctccggtt atacacggag cccgcgttac agcacgctgt tctggcttgg
     6361 tgatcaacta gttgactggg atgaacatga cggcataaag agtgctgtca ccggcctgct
     6421 ctcgagcgga ctttccggct atagcctgca gcattccgac atcggcggct acacggcaat
     6481 agacaacttc ctgctaaagt atcatcgctc caaagagttg ctcaggcgtt ggatcgagct
     6541 cggcgccttc accgtcgtct ttcgcaccca cgagggcaac aggcccaagg tgaaccacca
     6601 gatctactcg gacagagaga ccctccgcca cttcgaccgc ttcgcgaagg tctacgccgc
     6661 gtggaagccc tacaggatgg aactggtgaa gaaagcctcg gagacgggca tccctgtcgt
     6721 caggcatcca tttatccact atcccaacga tcccgaggtg ctcggtttga actaccagta
     6781 catggtcggc cctgagttca tggtcgcgcc tgtgctcgac cctggcagtg acagcgtaga
     6841 gatctacctc ccggcgggca agtgggtcca cctctggaca ggagagagat acggccttcc
     6901 gcaacgcggc gcctacgtga cgatcccggc tcctatcgga gagcccgccg tctactacaa
     6961 ggaaggttca gcggaaggaa cccgcttcag agaagagctc gaacgccggg ggctgctgta
     7021 acggtagaga ggtagaatgg ggtggttctt ttcctcgggg gactatcatg aagcgggaga
     7081 acgtaaccag cggcacggta tgggagagca gggtcggcta ctcgcgggcg gtccggcgcg
     7141 gtcgcttcgt gtacgtctcg gggaccaccg ccacggatgg gagcggggga tacgtcgggg
     7201 ttggggaccc ctacgcgcag accgtccagg ccataaacaa tatcaaggtg gcgctccagg
     7261 ctgtcggcgc agatctctcc gacgtggtga ggaccaggat ctacgtggtc aacatcgagg
     7321 actgggagaa gatcgggtct gcccatggcg tgtgcttcgg ccaggtccgt cccgccacga
     7381 gcctgatcga ggtcagccgc cttatagcgc ccgagatcct ggtggagatc gaagccgacg
     7441 ccctgatcga cgaatcgtag gggcgggttt gaaacccgcc cctacccgat ctccgaggcg
     7501 cggcccgcgg cgtcctccag ggcctgtagg gccgaggcct gatccatccc cgtgctcctg
     7561 agcaggtcga cggcggcgga ccggacctgg ccgacgagga cgctgatccc gagctcgtga
     7621 cgctcctcca ggatctcggt ggcggtcctg gccgcctcga gcgcccaccg gcgggcctcc
     7681 tctggcccct cgccatcctc cagaaaggcg gccagggccc tcacggcccg cgagaggtcg
     7741 aggacggcct cggacagttt cgggggtatc ctatctcccc gcctgatggc gttgtagacg
     7801 ccccgggcca ggacgcgggt gtttatgacg gccagctcga tccttatacc ggcactgccg
     7861 tagaactcga gttgcctcaa ggacctacga cgggtcggcg atagcctggc cgtctcgtga
     7921 cccgcctcga gcgcttcgtt gagggttctg accctgccat ctagctccct ggcacgttcc
     7981 aggacccttt cggcccgtct ctcgtctccg tccgctagca cggcggagat ctcctccagc
     8041 acagcgacca gctcctcgaa gacgggggcc gcggccctct cgaccaggcg ttcggggttg
     8101 atggggaaga ggtagttgat cgcgagcgca accccgcccc cgatgagcgc gtcgacaaag
     8161 cgttcaggcg agaagaggtc ctctggaggc tgcagcacta caactaggac cgccgagacg
     8221 gcagcctggt tgaccagtat ggttcctcct ccgaagaaaa ccgcagccgc catcgcaagg
     8281 agtacgacga cggcgatctg gagcgtcccg ttccctatga cgagcaccag gaggtcggcc
     8341 accatgagcc cgacggcgac cccgaagacg agttcgatgg cacgcctgtt cctctggccg
     8401 agcgttacgc tgaggcagat gacggcggct atgggggcga agaagggctg ctcgtgcccg
     8461 acgaacactt cggctacgaa gtaggccagg ctcgaggcca cagccgcctg caagatagac
     8521 cacacgctgg tcctgagacg gtcgtaccct gcagagagac cggcaccgac cctcgaaaac
     8581 aaaagtccct tctctcacgc cacccacacg accccagtga actatacacc ctcaggagga
     8641 cgagctaggt gttcacgaat cgtacgcctg cgtacacaag ggtacttcta tggaccatat
     8701 aatctcagga gatcaaagac gaggaggact tcatgggccg caggcgaata gaaccaggtc
     8761 agggactgga gtccgtctgg gattacccgc gtccgccgag ggtcgaggac gtggacaaga
     8821 aggtcaaggt cgtcttcggc ggcgtcacgc tcgcctacac cacgcgggcc aagcgcgtcc
     8881 tcgagacgag ccatccgcct gtctactaca tccctcgaca ggacatccag atggagttcc
     8941 tgaggggatc aggccgcact tctcactgcg aatggaaggg ccgcgccagc tactacgaca
     9001 tcgccacaaa ggacgagata accggcgaag cgaaatccga gaagggcgtc gcctggcact
     9061 atcccgaccc ggtgcctgag ttcgaaggac tgaaagatca cgtggcattc tatccgaaca
     9121 ggatggatgc ttgttgggtg gggggagaga aggtcgaggc gcaggaaggg gatttctacg
     9181 ggggttggat cacacccgac atagtcggcc ccttcaaagg cgcccccggc acctggggct
     9241 ggtagataca ccgtggatgc ctcgagtctt cgcaaggagt acaccagcgc gggcctggac
     9301 aaagccgacg tagaccccga ccccattgtc cagtttcaag agtggttcga gaaggtcatc
     9361 gatgctgacc tccacgagcc gaacgccatg atcctcgcca ccgccaccgc agacgggaag
     9421 ccctcagccc gcactgtact tctcaagggc tacgacgaaa ggggcttcgt cttctacacc
     9481 aactacgagg gacgcaaggc cgtcgagcta gagagcaatc cccgcgccgc gctcgtcttc
     9541 tactggggcg agttggagcg gcaggtgagg gtcgagggca cagtctcccg cgtctcagtg
     9601 gaggagtcgg acgaatactt cgccagccgt ccgcgcggaa gccggctcgg ggcatgggct
     9661 tcggagcaga gcagagtcgt ggacggtcgc cgcacgctcg aacagaagct gcattccctc
     9721 caggcggagt acgagaaccg cgaggttccg cgccctccgt tctggggcgg ttacaggatc
     9781 gaacctgaag cgatcgagtt ctggcagggt cgggaagatc ggctgcacga ccggctggtg
     9841 tatcgccgag aagatggagc ctggaggatc gagcgtttgc agccgtaggg aggcgtgagg
     9901 ccccgtcttg ctgctaagat gcgtgtcgtt gcatctcgat tcccaggaga ggagagctct
     9961 tgaagctggt tacgtattcc gtgggtggtg gggagactag cgtcgggtct ctggaaggcg
    10021 aggagataag gcccctcggc catgaggaca tgatcgagtt catcgagtac ggcgggagcc
    10081 cggagcctgg agaagatacg gtgccgctcg gggacgcccg catccacgcc ccgatttcaa
    10141 gacctcagaa aatcatcggc ataggcctca attacgagga ccacgctgct gagacggggg
    10201 cagatatacc cgagaagccc atcgtcttcg ctatgtaccc gaacgcgctc atagggccag
    10261 gcgaggccat caggataccc tccataaccg agcaggccga ctacgaggcc gagctcacag
    10321 tcattatcgg gagggctgcc cgcaacgtct ccgagtccga ggcgctggac tacgtcttcg
    10381 gctacacgaa cgcgaacgac gtctcttcgc gcgacctgca gttctcggag ggcggacagt
    10441 ggacgcgcag caagtctata gacaccttct gcccgttagg gccgttcatc gtgaccacag
    10501 acgagatcga agacccccaa gacctctcca tacgctgcat cctaaacggc gaggtcatgc
    10561 aggaagggac gacgcagaag atgatcttct ccgtcgccga gctcgtctcc ttcctctccc
    10621 agggcatgac cctcgtcccg ggcgacgtca tacttacagg cacgccccca ggcgtcggcg
    10681 ctgcccgcga cccgcaggtg tggttgaagg ctggagatga ggtgacgatc gagatacagg
    10741 gcctaggatc cctcaccaat cccgtcgaga aggactagat ctctcagaga aattccatgt
    10801 ctcgggtcgc cgattcactt ttttgccgaa ctcgtgaaaa agccggtgag ggggtacttg
    10861 gcctgctcga tagtcgatgg gctgatactc acgacgggct gtagaattgg cgggtaggcg
    10921 agagaaagta ccaagaccct ggtggcacca gagacgacat cataccggcc tgtgagggta
    10981 atcggcgtgc tccttatcct cgaggtgatc gggctccttg ttctcggcgg ctacgagttc
    11041 ttgcaggtcg actggagggg gatcaacctg cggagtccgc agaatcaggc cgtagaagtt
    11101 gcggtcttcg ccctcttcgt cccttccgcc gtcctgacgc tgctctcggc gctgagcttc
    11161 ctcttccttc gccgcagggg atggcttctg gccgcgatcg cgcagggcct gagcctggct
    11221 gtgtgcctgt ggctctactc tttgttccag cctggctatg tctacccgat catggcctac
    11281 tgcatactga tgatcctcta cctcaactcc cacgacgtaa ggatggtgtt ccgtcccgga
    11341 cagagccctc aggagtcgca tgaaagctga gagtccatgc gtggaggccg ccacggccct
    11401 tctccggcgt ttcgagcccg tgatccgctc gaccacaggg gacaagttct accctatgga
    11461 cgtcgaaccc tacgtgcggg cctgcagcct ctgggtgcag agtccgggtg aggaagccgt
    11521 ctgcatcgtg cccggtggga agctgagtct ggaccggctc gcgcagcaac ccatggacga
    11581 ggccggtgcc gtacatttcc tcaagttcac ggatgccgag gatctcgggc ctgacggtcg
    11641 tggcaggcgc ctgcgactgt tccgcagacg cgccaaggat cgcaaggagt cgacgagagc
    11701 tttcagggcc gggcggggcc gtctcgcccg cgtagggtac ttctcccgct tcgtcgacgc
    11761 cctttactcg atagcgctcc ttgcccgcgg aagggtgccg ggggaatcgg ccgccgcggc
    11821 ttcggtggcc tacgagcgca taatggccga acaggaacaa taccgctacc acgggcgcgt
    11881 actgcgccag gacggttgga tcgtactgca gtactggttg ttctaccctt tcaacgactg
    11941 gaggagcggt ttcttcggcg cgaacgacca cgaagccgac tgggagaagg tcttcatcta
    12001 cctctccgag tcggaggatg gggaggtaag ccctgagtgg gtggcctacg cggcccacaa
    12061 ctatacgggg gacaacctcc gtcgccgctg ggacgacccg gagctcgaga aggtggggga
    12121 gcatcccgtg atctacgttg gcgcaggctc ccacgccagc tactacgcgc cgggcgagta
    12181 cctgacggag ctgaccctcc ccctgccttc tcctctggca agggtgagtg gagccatccg
    12241 gactttctgg aagacgaggc tcggacagta cgtgggggat ggtgaggagg acggcgggcc
    12301 aggctacttc agcatcccct tcgtggacta cgcgcgcgga gatggtttga gcgtggggga
    12361 ggggggagac aggagctggg atgagcccag gctcatctcg gacccgcccc ccgactgggt
    12421 ctcgggctac cgcgggctgt ggggactcta cgccagggac cccttcgagg gcgaggatgc
    12481 ccccgctggc ccgatgtaca accgcgacaa gagcatcaac cgcgcctggt acgaccccgt
    12541 gggatgggcc ggactagaca aagtgcctcc cccgaccgag acgctagagg ccgtcctcga
    12601 acagcgggcc gacatcatct cgcggtgcga cgtcctaagg aacgagatag acgacaagag
    12661 ccggcagctc aaaaagctcg gcgccgagct cgcggcgatg tgcgacagat cccacctcga
    12721 cgcgccctac caggaaggga ccaaacgcgt cgccgagctc tcgggagagc tggaccggct
    12781 ccgtggaaaa ctcgccacgg acgaggccgt ttcagagtcc ctcgctgagt acgccggccg
    12841 tctgaaggcc ggcgagcggg acccggcccg ctcccacatc tcccgcgccc acaggccggc
    12901 ttccgaagac gagctgaggg tgagccgcgt ggccgaggcc tgggcggcca tcagcgtgag
    12961 cctgatgctc ataagcttcg tcgggatagc catgttcgag cgggaacacc tgatctccat
    13021 gctcgtcttc agcatcgcct tcttcgcgtt cgtcgaggcc ggcttcaggg gccgcctcgt
    13081 caacctggtc agcagcgcca acataggact cgccgtagtc gccacgctca tcatcgtcta
    13141 cgaattcttc tggcagctcg tagtcgccgc cgtgctcgtg ataggcatct acgtcctttg
    13201 ggacaacctg cgcgagctca ggagatgacg cacggaattc gagcggcagt taaaatgggc
    13261 tcactgtggc gcgcacgata agggtggttg tagcgaaggt tggcctcgat gggcacgaca
    13321 ggggggccaa gatcatcgcc cgcgccctcc gcgacgcggg gatggaggtc atctacacgg
    13381 gcctccacca gacccccgag caggtcgtcg aggcggccat ccaggaggat gtcgacgcca
    13441 ttggcatctc catcctctcc ggcgcccaca tgacgctcat accccgcata atcgaactcc
    13501 tcgaagagaa cgaggccgaa gacatcctcg tattcgtcgg cggtaccatc cccaaagaag
    13561 acatcacaag actaaaggag ctcggtgtcg gcgaggtctt taccccggga acaccgacga
    13621 agaaagctgt agagtatgtg aggcaggcta cgcctgccag ctagtcagca cgccgccttg
    13681 cggcggcttg tcagctaatc agccaaaagt tgaagtgccg acatgctgaa atgctgccga
    13741 ccggagggag gcaaatggat tacgtttcgg tacaacacga cgacgatgtc gccgtcctga
    13801 ccatcgaccg ccaggagaag ttgaacgccc ttgacatgca ggtggtcgag gagatagggc
    13861 aggcactgct agatctggag gcagaagggc cgcgcgccat cgtcgttacg ggagcggggg
    13921 agcgggcctt tgtcgctggg gccgacatca gggccatgag catcatggaa cccctggagg
    13981 ccaagcgctt ctccgagatc ggccatgcag cgatggccct cctcgacagg agccccgtcc
    14041 ccaccattgc cgccgtgaac ggctacgcgc tcggcggagg atgtgaggtc gcgctcgcct
    14101 gcgacatcag gatagccgcc gagaacgcga ccttcggctt ccccgaggtc agcctcggga
    14161 tactccctgg catgggcggc acccagcgct tgccgcgcct gatcgggccc gcgctggcca
    14221 aggagttgat cttcaccggc cgccgcataa gcgccgaaga ggccagggag ataggtctcg
    14281 tcaaccgcgt cgtcgccgag ggtgaagcgt tgaacgcggc cagggaattg gcggccgaga
    14341 tctctgccaa cggtcctctc gccgtcaggc acgcaaaggc ggcggcgaac cgcacgctcg
    14401 acgtcgacct cgtaagcggc ctagagttcg aggctgacca gttcgccctg ctcttcgcca
    14461 ccgaggacgc ccgcgaaggc atgaacgcct tcggtgagaa gcgcaaaccg gagttcgagg
    14521 gaagatagcc cacgagacca aaatacgggt ccgctacagt gagatcgacg cccaggggca
    14581 cgtaaacaat gccacttacc tctcctactt cgaagtcgga cgcgtagagt ggctgcgcgc
    14641 cacaggccac tcttacaggg agatggaggc acggggtcgc ggcctcgtcg tcgtcgaggc
    14701 gctcctccac taccgtagac cggccttctt cgacgacgag ttgaccctga gcacacacct
    14761 cgccgatatt ggcaaggtct ccctccgctt cgattacgag gtactcaagg acggagaagt
    14821 gctcgtcacc aggcacacgc gccacgcctg cgtcgacctg gccacaggca aacccatcag
    14881 gatgccggag gaacttctca tgctcgcaaa gggcacgccg tagtccggaa tgcgcaggat
    14941 cactaccgca gtcgggggca agaacgcaaa gaagcgagcg gtggtggcga ggaagcttgc
    15001 ggtgctccta cacactttgt gggttagcag ggaggcctac gacgggtaaa gacaccgaag
    15061 cgggtattct gtacggcagt aggtgattgc gttcccggca tgcagagatc tcgcatcatc
    15121 gcggaaggat cgtactgaga aaatgtgagc ctgcagtcgt cctccctgtt tattggctca
    15181 ctgaacggtg accttgctgg cgccgccgga gacctcgatc tcgtagcggt cggagacact
    15241 gtcgtagccg gggctcctga gccggagctt gccgcccacg gcgtcgaagc tctgctcgtc
    15301 gaacgtcagc ttgctgacgc cgcctatcat gctcagccgc gcctcgaccc ctgccggacg
    15361 gcgtatgttc acctcgctgg cgcccccggt gagacgtacc gggaccaccc ccgacggctc
    15421 cggaagcgtc aggtcgacgt cgctcaaccc ccgcgtcaag acgaaggagg ccagcttcaa
    15481 tccgctgagg tccgcctcga ccttgtacgc tccgccgcgc acctcgacct cccagggcac
    15541 cgcggcatta agcgttacct ccccccggac cggctgcgcc actcgaagag cccgctaaag
    15601 cggcggggat aacgaaaggt cacggtgccg tctgccactt cgaccttcgg tacaggcccc
    15661 tcgaagcgtg cccggtagag gtcatccatc ccggaggcgg cccggagggt gagtcgggaa
    15721 accccgttgg cgaacatcat ccgcccgctc tgtgccgagc ctaaaggagc cgagaactcg
    15781 cccccttcgc ccccttttga agcggcccgg atcctggcgg tttcctcccg ggtcatggcg
    15841 ttggccttcc gcatgaggtc cacgaagagc gcgagatccc gcgccggggg agatcttcgg
    15901 cttccttggt ccgaacggcg cgggcaagac cacgacgatt cggatgctcg ccaccctgct
    15961 acgcccgcac tcgggcacgg cgcgggtttt cggccacgac gtcgttgggg agtccgacgc
    16021 ggtacgcgat cgggtaagcc tcaccgggca gttcgcctcg gtagacgagg atcttaccgg
    16081 actggagaac ctcgttctta tagcccgcct cctcggctat tctcgcaagg gtgcgaaagg
    16141 gcgcgccgga gagctcctcc atgccttcgg gctctccgaa gcggcgggcc ggctggccaa
    16201 gacctattcg ggaggcatgc ggcgcaggct cgacatcgcg gcgtcgatcg tcgtcacgcc
    16261 ggacctcctg ttcctcgacg agccgaccac cggcctcgac cccaggaacc gcaaccaagt
    16321 ctgggacatc gtccgcgcca tgtcctcggg gggaacgact gtccttttga ccacgcagta
    16381 cctcgacgag gcggaccgac tcgcggaccg cctcgccgtc atagacaacg ggaaggtgat
    16441 cgccgaaggc acccccggcg aactgaagaa ctcggttggc tcggacgtcc tgcgcgtcag
    16501 gctgcgcgaa tcggaagccc gccccagggc cgagcgagtg ctttcgcggg cgctcggcgt
    16561 cgcggtggag accccttctg acccggttgc cctcctggcg agggtctccg aacccgagcg
    16621 ggcacccgac gccctcgcag agcttccccg cgccggcatc gccgtgacgg acttctcgct
    16681 cgggcagccg agcctcgacg aggtgttcct cgccctgacg ggacgccccg ccaaacaaga
    16741 aacctacaaa gaagaggagg aagccgtatg accgatccgg ccggtgtgag ccgtaccacc
    16801 atcgccgagg aagcgctgcg ttcggcgctc tcgtccggcg agaggcccaa gcgtccgaac
    16861 gctctctcgg cctcgctcac gttcgggtgg cgggcgctgc tcaagataaa gcacgtccct
    16921 gagcagctct tcgacgccgt ggggaacccg atcatcttta cgctactgtt cacgtacctg
    16981 ctcggcggcg ccatagccgg ctcgaccggc gcctacctcc agttcctcat ccccggcata
    17041 ctggtgcaga ccgtcatact catcaccgcc tacacggggt tcaacctcaa caccgacatc
    17101 tcgaagggcg tttccgaccg cttccggtcg ctcccgatat ggcgaccttc cgtcatcgtg
    17161 ggtgcgctgc tcggcgatgt ggcgcgttac gcgttggcgt cggcgatcgt cctcacgctt
    17221 ggcctggtcc tcggctttcg gccggagggc gtggttggcg tactgctgtc ggtggcgttg
    17281 ctcgtcgtgt tcgcgtttag cgtctcgtgg atctggacgg ccttggggct cgtcttgcgg
    17341 acgccaaact ccgtgttgtg ggtgagcacg ctcgtcatgt tccccctcac cttcgccagc
    17401 aacattttcg tcgagccaaa gacgatgcca ggctggctcg aaacggccgt cggctacaac
    17461 ccgatcacgc acctggtgag cgcgatccgc ggcctgatgt cagggacgat gccggcgggg
    17521 cacatctcct ggctgctcgt ggggtgcgcg atgctcgtcg cagtctttgc ccctatcact
    17581 gtgcgcctct accggaacaa ggagtgagcg gatcacctca attctcctcc gttgcctcca
    17641 caggtcggcc ctcacggtgg atgggctctg ctaaactcag cctccgtgaa tcctctgctc
    17701 ggacagcacg cataccggct ctccgaagcc ggggtagcag gtaatcgaac ctcacacggt
    17761 gccgagaaca acctccgcaa gataaggctg ctcctcgagc acgacgcttc caacacgttc
    17821 gggatggagg atctgctaca ggatgtggga ttcgatgagg cctacgacgc tgtcgcccat
    17881 cagatcggca acccacctga cagggaggag aatccggagc ggggatgcat agacccggcg
    17941 cgtacggctg cagggctagt cgaggcgggt gggcggatag aggccgtcgc cgggtcgggt
    18001 gggcgattga tcttcgccac cgggcatcct ggagcaatga tcctctacta cctcggtctc
    18061 gcccggtggg cgcgagagct tggcggcgat gccctcaccg ccgagacgcg cggacgctac
    18121 gagcgaggag tgtccctaga ctgggcggag tcggtggccg ccctcggcga cggggccagc
    18181 ctgttccaca cccacgatcc cgagcccatg acggatgtat tgcggcagac gggagcggtc
    18241 gaccttgtgg tcgccgacca cgggttcgcg ggggctgcga tcgccaccgg cgtccccacc
    18301 atcgtggtga tggacaccaa cgatccagcc ttcgccgtag tcgccggtcg aggcgccgac
    18361 gtaacggtcg tgcccatgga cgacaaccgg ccgctgaatg cctactcgac ggccctcgac
    18421 gt
//
LOCUS       DASTKM010000093         5404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_196906, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000093 DASTKM010000000
VERSION     DASTKM010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196906"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFQ10_03800"
     CDS             complement(<1..254)
                     /locus_tag="VFQ10_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964496.1"
                     /translation="MDPHEQSDRDFSRARRRAFLRRIGAYLRRDPASNQLLSFDEVKG
                     ALGAISQVYLGLREVPVSKIVGSVGRHRDFDRAFLPSKPDL"
     gene            complement(321..938)
                     /locus_tag="VFQ10_03805"
     CDS             complement(321..938)
                     /locus_tag="VFQ10_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9964497.1"
                     /translation="MKILCVSDRVERMLYGPNLTSYARGVEAVISCGDLPFDYLEYIV
                     TFLGVPVYYVLGNHDPGPDGPEYPGGCTPLDGRVVEAEGKGGGVVLAGLSGSPVYSGG
                     PNQYTERHMRRRARTLSVRIRCRTLVGRPKPTIFVAHSPPFGLGDQEDVAHVGFESFL
                     DLIDRYHPPLWLHGHVHLYGPDKERVRQKGDTKIINVYSHRILEV"
     gene            1134..1526
                     /locus_tag="VFQ10_03810"
     CDS             1134..1526
                     /locus_tag="VFQ10_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9964498.1"
                     /translation="MAGPETPKLMVDVVIPSEAGVVLIRRGSEPFEGKWALPGGFVDV
                     GETVREAAVREVAEETGLAVEVSRLVGVYSEPDRDPRGHNVSVAFLARVLGGQMQAAS
                     DAAEVEVLDPNTVELAFDHSRIIADAMV"
     gene            complement(1654..2526)
                     /locus_tag="VFQ10_03815"
     CDS             complement(1654..2526)
                     /locus_tag="VFQ10_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET9964499.1"
                     /translation="MDQTRVVLDCDTANEVDDQFAIAHALGLPEGTLDVRGVISVHNT
                     TAHGPGSRDMYQEEAVRVVALCASDVPCVPGADRPMESMEEPVPSAGLDFLVEEARLS
                     PLTVIATGPATDVASLLLAAPESRENVRVVWLGGFGDQASYRVWQNRMGELNGRADIS
                     AWRTLLKEPVDLLHIPTWPAPAKILVDPASFGEELRALGRPIASYLAGILQPLAAEYE
                     YRSGKELWNLACVAAVADPGSITSEPLAVPTLDAAGAHDFTQPGREVDVVLDLDERRV
                     LAGLMEALARHPEG"
     gene            2643..3101
                     /locus_tag="VFQ10_03820"
     CDS             2643..3101
                     /locus_tag="VFQ10_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET9964500.1"
                     /translation="MDWEIIFWVALAALAFVGELLTVSFFLLFFSLGAVVGLVAALLG
                     FSITVQAVGFLAASVLSMVILRPAFLNRLALGSGESYLGHRGITGKRAVVTEEIEAGG
                     KGMVRVGSGEFWTARSLHPDERIERGTRVRVLDTDGLTALVDTVEKEEKS"
     gene            3098..4105
                     /locus_tag="VFQ10_03825"
     CDS             3098..4105
                     /locus_tag="VFQ10_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9964501.1"
                     /translation="MTGLIGLAVLALVVFLVAARAVRIIPQSRVAIVQRFGRYHRTAE
                     SGLTLVVPIMDRMLPKTDLREQVVAFQPQAVITSDNVGMQISTVVYYQVVDARAAEYE
                     VANFHLALEQITQTTLRNVIGNLILDKTLTSRDEINAKLRTVLDEVTEKWGIRVTRVE
                     LKEIIPPRDIQQAMEKQMQAERTRRAAILTAEGDKRAAVLKSEGEKASAILRAEGDQR
                     AAVLRAEGEAEAYRNVQAAQIEMTGKLFERLETSDLSPESLRYLYLKALPEMAKGPAS
                     KLFVVPSEIQDLAGKLGALAGGASLAGEDGPQDEGEQRRIQARGADPNGELPARRENQ
                     L"
     gene            complement(4140..5333)
                     /locus_tag="VFQ10_03830"
     CDS             complement(4140..5333)
                     /locus_tag="VFQ10_03830"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET9964502.1"
                     /translation="MNDQMHTGIAEATRLTQQGRLDEATAAIQRALGGTFAPAAQEGP
                     GDTNEPREVISRLVRETPAGGRTPTPRRPGAADWTAPGPTLLTHGVQLPPGMKAGKLA
                     SVSVPEGGRFVERSYSNSAGTRSYKLYIPSSYTGQAVPLIVMLHGCTQSPDDFAKGTR
                     MNELSEEHTFLVAYPAQASNANMQKCWNWFQSADQQRGRGEPSLIAGITSQVIDEYEV
                     AEGRVYVAGMSAGGAMAAIMGSTYPDLYAAVGVHSGLAPGSAHDMSSAFTAMRQGGPV
                     VAHNDVAEGEHWEILPTILFHGDGDTTVHPRNGDRLLAHLARGGNGSSPRVRTRQGQV
                     PGSYKYTRFTYRDAEGRDLVERWSVHGLGHAWSGGSYPGSYTDPRGPDASAEMVRFFE
                     QHHKR"
BASE COUNT          970 a         1681 c         1757 g          996 t
ORIGIN      
        1 aggtcgggtt tggaaggcag gaaggccctg tcgaagtctc tgtggcgtcc gacgctaccg
       61 acgatcttgg agactggcac ctcgcgcagc cccaggtaga cctgcgagat ggccccaaga
      121 gcccccttga cctcgtcgaa ggagagcaac tggttggagg ctgggtctct gcgcaggtac
      181 gcgccgatcc ttcgcagaaa ggcccttctg cgggcgcgtg aaaagtctct gtcggattgc
      241 tcgtggggat ccataaacct cactcatgga ccaatctaca gtcccccggc cttgtcaaca
      301 agggcgcgga acgggaaaag ttacacttcg aggattcggt ggctgtagac gttgattatc
      361 ttggtgtctc ccttctgtct gacccgctcc ttatccggcc cataaaggtg tacgtgtccg
      421 tgcagccaca agggaggatg gtacctgtcg atgaggtcaa ggaaggattc gaagcccacg
      481 tgggccacat cctcctggtc cccgaggccg aaaggcgggg agtgggcaac gaagattgtg
      541 ggcttcggtc ggccgacgag ggttcggcaa cggatcctga ccgagagcgt cctcgcccgg
      601 cggcgcatgt ggcgctcggt atactggttg gggccgcccg agtaaacagg agacccagag
      661 agccccgcaa gcaccacccc gccgcctttg ccctccgcct ccacaacccg tccgtccagc
      721 ggcgtgcagc caccggggta ctcggggcca tcggggcccg gatcatggtt accaagcacg
      781 tagtagacag ggacgccgag aaaggtgacg atgtattcga ggtaatcgaa aggcaggtcc
      841 ccgcaggaga tcaccgcctc gacgcccctg gcgtaggagg tcaggttcgg accgtacagc
      901 atcctctcca cccggtcgct gacgcaaagt atcttcatgt gtatattcct gtcaccctgc
      961 agagcatacc ctttttcgac ggggatcccc ggtcttcgaa gcgaacggtg ccgggaccgc
     1021 ggggtaacgc ggcccggatg cgggccgcgt tgcccctggc ggcacgaacc aacataatgc
     1081 accacgacac ggcgtaacct ttcgatgcgc cgggcacgca ggaggtgcga accttggcag
     1141 gaccggagac cccgaagctg atggtcgacg tggtgatccc ctctgaggcg ggggtggtct
     1201 tgatccgccg tggcagcgaa cccttcgagg ggaagtgggc gctgccaggg ggttttgtgg
     1261 acgtgggaga gacggtgagg gaggccgccg tacgcgaggt tgcggaggag acgggcctcg
     1321 ctgtagaggt ctcgcgcctc gtcggcgttt actccgagcc agaccgcgac ccgcgcggcc
     1381 acaatgtcag cgtcgccttc ctggcccgcg tcctgggcgg ccagatgcag gccgcctctg
     1441 acgccgcaga ggtcgaagtc ctggacccca acaccgtcga actggcattc gaccacagcc
     1501 gcatcatcgc cgacgcgatg gtgtgacatt tcagccagtc agcacgccgc ctacggcggc
     1561 tctcagccaa gcagcctttg tttgctcaag ttctgacaaa cggaggcgaa gccgcagcgt
     1621 gctgactggc tgacgagcgc agggagcgga aggttaacct tccggatggc gtgctagagc
     1681 ctccatgagg cccgcgagta ctcgtcgttc gtcgaggtcc aagacaacgt ctacttccct
     1741 tcccggctgc gtgaaatcgt gcgcacccgc cgcatccagg gtcgggacgg ccagtggttc
     1801 tgaggttatg gatcctggat cggcaacggc ggcgacgcag gccaggttcc acagttcctt
     1861 tccggaccgg tactcgtact cagcggccag aggctgcagg atcccggcca ggtacgatgc
     1921 gatgggccgt ccgagcgcgc gcaactcctc tccgaatgat gccggatcga ccagtatctt
     1981 cgccggcgcg ggccacgtgg ggatgtgcag gagatccacg ggttccttca gcaacgtgcg
     2041 ccaggccgag atgtcggccc tgccgtttag ctctcccatc ctgttctgcc agactctgta
     2101 gctggcttgg tccccgaacc ctccgagcca gacgacgcgc acgttctccc gcgactccgg
     2161 cgccgccaga agcagcgatg cgacgtccgt cgccggtcca gtggcgataa cggtcagtgg
     2221 gctcaggcgc gcttcttcca ccaagaaatc gaggcccgca ctaggcaccg gctcctccat
     2281 gctctccatc gggcggtccg ctccgggaac acagggcacg tcgctcgcgc acagggcgac
     2341 cacccgcacg gcctcctcct ggtacatgtc gcgcgagcca ggaccgtggg cagtggtgtt
     2401 atgcacggag atcacgcccc gcacatccag cgtcccttcc ggcaacccga gcgcgtgggc
     2461 tatagcgaac tgatcgtcta cctcgttcgc tgtgtcgcag tcgaggacta cgcgtgtttg
     2521 gtccatgatc ttctctcccg tccgattcga tggtcatggc cgtattgtag gcaggtggga
     2581 tgggcactgg ttggttccgc gcttgcggcg gtggtgtaaa cttacgtcag gaggcacgag
     2641 acttggattg ggagatcatt ttctgggttg cgctggcggc tctggccttc gtcggtgagc
     2701 ttctgaccgt ctctttcttc ctgctgttct tctcgctcgg cgctgtcgtg gggctcgtgg
     2761 ccgccctgct tggattcagc atcacggtcc aggccgtagg gttcctcgcg gcctcggttc
     2821 tcagcatggt catcctgcgt cccgccttct tgaaccggct cgcgcttggg agtggggaga
     2881 gttacctcgg acacaggggg atcacgggca aacgcgcggt cgtaacggag gagatagagg
     2941 ccggaggtaa ggggatggtg agggtaggca gcggtgagtt ctggacggcc cgctcgttgc
     3001 atcccgacga aaggatagag cgaggcacca gagtgagggt gcttgacaca gacgggctta
     3061 ccgccctggt cgataccgtg gagaaggagg aaaagtcatg accggcctca tagggttggc
     3121 agtactggcg ctcgtggtct ttctggtggc tgcgagggcc gtcaggatca tcccccagag
     3181 cagggttgcg atcgtgcaac gcttcggccg ttaccaccgt acggccgaga gcgggctgac
     3241 cctcgtcgtc ccgataatgg accgcatgtt acccaagacg gacctgaggg aacaggtcgt
     3301 tgccttccag ccgcaggcgg tcatcaccag cgacaacgtc ggaatgcaga tctcgaccgt
     3361 tgtctactac caggtcgtcg acgcgcgggc cgccgagtac gaggtcgcaa acttccacct
     3421 ggcgctggag cagatcaccc agacgacctt gcggaacgtc atcggtaacc tcatcctcga
     3481 caagaccctt acttcccgcg acgagatcaa cgcgaagctg cgtacggttc tcgacgaggt
     3541 aacggagaag tggggcatca gggtgacgag ggtcgagctg aaggagatca tccccccgag
     3601 ggacatccag caggcgatgg agaagcagat gcaggccgaa cggactcgcc gcgccgctat
     3661 cctgaccgcc gaaggcgaca agcgggccgc cgtcctgaag tccgaaggcg aaaaagcgtc
     3721 cgcgatcctt cgcgcggaag gcgatcagcg ggcggccgtg ctgcgcgccg agggtgaggc
     3781 cgaggcctac cgcaacgtcc aggcagccca gatcgagatg acgggcaagc tcttcgagcg
     3841 cctggaaacc tctgacctct ctcccgaatc actccgttac ctctacctga aggctctccc
     3901 cgagatggcc aaaggaccgg cgagcaagct cttcgtcgtt ccttccgaga tacaggacct
     3961 cgcagggaaa ctcggagctc ttgccggcgg agcctccctc gccggcgaag acggcccgca
     4021 agacgaaggt gagcaacgcc ggatccaggc tcggggcgca gaccctaacg gcgagcttcc
     4081 ggcacggcgg gagaaccagc tgtagagcgg ggtatgggcg gagcacggtc ccggcctctc
     4141 taccgtttgt ggtgctgctc gaagaatcgc accatctcgg cagaggcatc gggccctctg
     4201 ggatccgtgt acgagcctgg gtagctcccg cccgaccagg catgtcctag cccatgcacg
     4261 ctccaccgct ccacgagatc tcgaccttcc gcgtcacggt aggtgaacct ggtgtacttg
     4321 taactgcctg gcacctgacc ctggcgtgtc cttactcgcg gtgacgagcc gttcccgcca
     4381 cgggctagat gggccagaag gcggtcaccg ttgcgcggat gaacggtagt atcgccatca
     4441 ccgtgaaaca atatcgttgg taaaatctcc caatgttctc cttcggcaac atcgttgtgt
     4501 gcgactaccg gtccgccctg gcgcatcgcg gtgaaagcgg aagacatatc gtgggctgat
     4561 ccaggggcga ggcctgagtg gacgccgact gcggcgtaaa ggtcgggata ggtcgagccc
     4621 ataatagcgg ccatcgctcc tcctgccgac atcccggcta cgtacaccct gccttcggcg
     4681 acctcatact cgtctatcac ctggcttgtg atacccgcaa tgagcgacgg ctcgccccga
     4741 ccgcgctgct ggtccgcgga ctggaaccag ttccagcact tctgcatgtt ggcgttgctc
     4801 gcttgcgcag gataagcgac caggaaggtg tgctcctcgg agagttcgtt catgcgggtg
     4861 cccttagcga agtcgtccgg gctctgggtg caaccgtgca gcatgacgat caggggaact
     4921 gcctgtccag tgtagctgct cgggatgtac agtttgtagg agcgggttcc ggcgctgttg
     4981 gagtacgaac gctcgacgaa gcgtcctccc tcggggacgc tgaccgaggc gagttttcca
     5041 gccttcatac cgggagggag ttgcacaccg tgggtgagca gggtcggccc aggagccgtc
     5101 caatcagccg cgccaggccg gcggggggtc ggcgtacgac cccccgccgg agtctctctt
     5161 acgagacggg agatcacctc cctcggctcg tttgtgtcgc ctggaccttc ctgcgctgca
     5221 ggggcgaacg tgccgccgag ggcgcgctgg atagcggcgg tcgcctcgtc gaggcggccc
     5281 tgttgcgtga ggcgtgtggc ctcagcgatg ccggtatgca tctggtcgtt catgatagtg
     5341 ctcctctcga tgtcaggaag attggatcct gcccttcagc gccgccttca cggccgggct
     5401 cgcc
//
LOCUS       DASTKM010000094         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_197283, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000094 DASTKM010000000
VERSION     DASTKM010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197283"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFQ10_03835"
     CDS             complement(<1..512)
                     /locus_tag="VFQ10_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HET9964503.1"
                     /translation="MPDLLVTTDGQPDRDGTLIKVTPQSAGWEYVGFEVLRLEAGRSV
                     SRRTGGDEVCLVPLSGTCTVSAGDERWEDIGGRESVFEGPPYAVYLPPGTGYKVEAST
                     ELEIAVASAPAGRGVDPMMVRPEDIEVEIRGSGNMEREIRPILMEDRRAERLLVVEVL
                     TPNGHWSSYPP"
     gene            complement(528..2408)
                     /gene="iolD"
                     /locus_tag="VFQ10_03840"
     CDS             complement(528..2408)
                     /gene="iolD"
                     /locus_tag="VFQ10_03840"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HET9964504.1"
                     /translation="MVTVRLTAAQALVRFLTEQYVERDGIERHFFAGCFGIFGHGNVA
                     GVGQALQQYSDGLPYYQARNEQAMVHTASAYARQKDRLQAFACTTSIGPGATNMVTGA
                     ALATINRLPVLLLPGDVFASRGPDPVLQQLETPHDGTLSVNDCFRPVSRYWDRVMRPE
                     QVMPAALAAMRVLTNQAETGAVTLALPQDVQTEAFDYPETFFEKRIWHVGRPLPDAGA
                     MERAAAMIRASRRPMIVAGGGVIYSEAAEALRRLCERTGIPVGETQAGKGSLPYDHPC
                     ALGAVGATGTFAANRAAREADLVIGVGTRWSDFTTASKTAFQNPEARFVNINIADFDA
                     HKHSGVALVGDARSTLEVLDDAVMGYEVEPGYREQNAEESREWDREVGRLYALGHGPL
                     PAQSEVIGAVNSFSDPRDVVVCAAGSMPGDLHKLWRTRDPKGYHVEYGYSCMGYEISG
                     GLGVKMAAPEREVYVMCGDGSYLMMNSEIVTSIQEGHKLTMVLVDNSGFSSIGALSRS
                     VGSDGFGTHYRYRKRGSIGLDSEEAPGDILPVDLAKGAESLGAYVIRTENIEDLKKAL
                     SDAKDVDRTVVIHIPVDRYEGVPDYDSFWDVPVSEVSEMESVTSAREEYAENKKAERR
                     YL"
     gene            complement(2571..>3152)
                     /locus_tag="VFQ10_03845"
     CDS             complement(2571..>3152)
                     /locus_tag="VFQ10_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9964505.1"
                     /translation="LDFDAISAVPLFWITGTGLSDEPSRTATLAALEHRPSGITVFDL
                     DYRPTLWASEEEAAHCYREALRHATVAVGNQTEVEVAVGTGDPFEASEGLLDLGIELA
                     IVKRGPDGVLARTKDGVAEAPPIEVEIVNGLGAGDAFGGALCHALLSGWDAERTIRYA
                     NAAGAIVASRLACADDMPTREEVERLLEKSSNA"
BASE COUNT          534 a         1021 c         1002 g          595 t
ORIGIN      
        1 ggcgggtagc tcgaccagtg cccgttgggg gtcaggacct cgaccaccag gagccgctcc
       61 gccctgcggt cctccatcag gatcggacgt atctcccgct ccatgttgcc cgagccgcga
      121 atctccactt cgatgtcctc gggccttacc atcatcgggt cgaccccccg cccggcaggc
      181 gctgaggcga ccgcaatctc caactctgtc gaggcttcta ccttgtaccc ggtaccaggc
      241 ggcagataga ccgcataagg aggcccttcg aaaacgctct ccctgccacc aatatcctcc
      301 cacctctcat cccccgccga cacggtgcac gttcccgata gcgggaccag gcacacctca
      361 tcgccccctg tgcgccgact cacgctccgg cccgcctcga gcctgagcac ttcgaaccct
      421 acgtactccc agcccgcgga ttgcggcgta acctttatca gggtgccgtc gcggtcaggc
      481 tggccgtcgg tggtaacgag caggtctggc acgccgaaag ctcctttcta gaggtatctg
      541 cgttctgctt tcttgttctc cgcgtactcc tcgcgggctg aggtgaccga ctccatctcg
      601 gagacctcgg agacggggac gtcccagaag gagtcgtaat cggggacgcc ttcgtaacgg
      661 tcgacgggga tgtggatgac cacggtcctg tcaacgtctt tggcgtctga gagggctttc
      721 ttcaggtctt cgatgttttc ggtccggatc acatacgcac cgaggctctc ggcacctttg
      781 gccaggtcga cgggcaggat gtcgcccggc gcctcttccg agtctaggcc tatggaccct
      841 ctcttgcggt agcgatagtg ggtgccgaag ccgtccgagc cgacggagcg cgagagtgcc
      901 ccgatagagg agaacccgga gttgtcgacc aggaccatgg tgagtttgtg gccttcctgg
      961 atggaggtaa cgatctcgga gttcatcatc aagtaagaac cgtcgccgca cattacgtag
     1021 acctcgcgct cgggggccgc catcttcacg ccgagcccgc cggagatctc ataccccatg
     1081 cacgagtagc cgtactcgac gtggtagccc ttggggtcgc gcgtcctcca gagcttgtgc
     1141 agatcccccg gcatggaacc cgccgcgcag acgacaacat cccggggatc ggagaaggaa
     1201 ttgacagccc ctatgacctc gctctgggcc ggcagcggcc cgtgaccaag ggcatagagc
     1261 cgcccgacct ccctgtccca ctcccgcgac tcctctgcgt tctgctccct gtagcccggc
     1321 tcaacttcgt agcccataac ggcgtcgtcc aatacttcga gtgtggagcg ggcgtcaccg
     1381 acgagggcga ctcccgagtg cttgtgtgcg tcgaagtcgg cgatgtttat gtttacgaag
     1441 cgagcctcgg ggttctggaa cgccgtcttg gacgccgtgg tgaagtctga ccagcgggtg
     1501 ccaaccccga tgacgaggtc ggcctcgcgg gccgcgcggt tcgccgcgaa ggtacccgtt
     1561 gcgccgacgg cgccaagggc gcagggatgg tcgtagggca gggagccttt gcccgcctgt
     1621 gtctcgccta caggtatgcc cgtgcgttcg cacaggcgcc gcagggcttc tgcggcctcc
     1681 gagtagatga cgcccccgcc ggccacgatc ataggccggc gggaagcacg gatcatggcc
     1741 gctgcgcgtt ccatcgcgcc tgcatcgggc agagggcgtc ctacgtgcca gatccgcttc
     1801 tcgaagaacg tttcgggata gtcgaaggcc tctgtctgca cgtcctgtgg gagtgcgagg
     1861 gtaacggccc ccgtctcggc ctggttcgtg agcacgcgca tcgcagccaa ggcggcaggc
     1921 atcacctgtt cggggcgcat taccctatcc cagtaccgag ataccggcct gaaacagtcg
     1981 ttgacggaga gggttccgtc gtggggggtc tcgagctgct gcagaacggg gtcaggtccg
     2041 cgggaggcga agacgtcgcc aggcaggagc agtacgggca ggcggttgat cgtggctagg
     2101 gccgctcctg taaccatgtt ggtggcgccg ggtccgatgg aggtggtgca ggcgaaggcc
     2161 tggaggcggt ctttctggcg ggcgtaggcc gaggcagtgt gtaccatcgc ctgctcgttg
     2221 cgggcttggt agtaaggaag gccgtcagag tactgctgga gtgcctggcc gacaccggcg
     2281 acgttaccgt ggccgaagat accgaagcat ccggcgaaga agtggcgttc gatcccgtct
     2341 ctctctacgt actgctcggt caggaagcgc actagcgcct gggccgccgt caaccttacc
     2401 gtgaccaaga gatcaccccc ttcccgcgct ttgcgacagg atagaacctt ccgcacagca
     2461 aggcttcagg ctacaggttt caagtatcag gatcggcatg ggacagtcac ctctctgagg
     2521 agagctatca tgtctcagct cttgggaaat gcactctggt ccgtacactc tcaggcattc
     2581 gaggacttct ccagcagtcg ctccacctct tcgcgggtcg gcatgtcgtc ggcgcaagcc
     2641 aggcgagagg cgacgatggc tcccgcagcg ttggcgtagc ggatggtcct ctcggcgtcc
     2701 caccccgaga gcagcgcgtg gcagagcgcc cctccgaagg catcacccgc gccgagcccg
     2761 ttgacgatct ctacctctat tggtggggct tcggctactc cgtctttagt gcgggcgagc
     2821 acgccatcag gtccccgctt gacgatcgcg agttctatcc cgaggtcgag tagcccctcc
     2881 gaggcttcga aaggatctcc tgtgcctacg gccacctcga cttcggtctg gttgccgacg
     2941 gccaccgtgg cgtgccgcag cgcctccctg taacaatggg ccgcctcctc ttccgaggcc
     3001 cagagcgtcg gcctgtagtc gaggtcgaag accgtgatgc cggagggcct gtgctcgagg
     3061 gcagcgaggg tcgccgtacg actcggctcg tcggagagac cagtaccggt gatccagaac
     3121 aacggcacgg ccgagatcgc gtcgaagtcg ag
//
LOCUS       DASTKM010000095        11590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1430889, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000095 DASTKM010000000
VERSION     DASTKM010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430889"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..703
                     /gene="rplT"
                     /locus_tag="VFQ10_03850"
     CDS             89..703
                     /gene="rplT"
                     /locus_tag="VFQ10_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET9964506.1"
                     /translation="MARTARSVHARKKRRKVLEQAKGYRGTKHSSYKRAKEQVWKSGV
                     YAYEGRKQRKRDFRALWIQRINAGAREHDLSYSRLIHGLRLAEIDLDRKILADLAVTE
                     PEIFGAIVAQAKNALDGKPVDRRVEVERSAEPEQPISSAARPPEAPAEQAEEAETSED
                     GLQATDAASRKAEELNVNLSSVEGTGQGGRITVGDVKKAARGVE"
     gene            711..1397
                     /locus_tag="VFQ10_03855"
     CDS             711..1397
                     /locus_tag="VFQ10_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9964507.1"
                     /translation="MPGTPAISPKRAARLKTKKHREAERLFLAEGAAVLEEAPTAPLQ
                     VFDEVGQLRRVSTLKTPSGPVGVFPFLDVTATELLASVRRVVLLHGVQDPGNVGTAIR
                     SAHAFGAGVALSSGSADLYNPKTVRASMGSIFHAPVARELESVDFLAEAGSAGFELVA
                     AVPEAGVTPRSLSRGRLVVCIGAEGSGLPEEVVRTCEMRVGIPSLAPSLNASVAASIL
                     LYEAYSRVLP"
     gene            1407..2435
                     /gene="pheS"
                     /locus_tag="VFQ10_03860"
     CDS             1407..2435
                     /gene="pheS"
                     /locus_tag="VFQ10_03860"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET9964508.1"
                     /translation="MTVSERIEQLKSEALSAVSSSASTADLEDVRVRFLGRSAELTEI
                     KKAIGTLSPEERKEVGRSSNLASREIEAALGSRTEEVAAREQEERLRAEAVDVTLPGV
                     PFPRGHLHPSQIVIDDVVDFFVGLGYRVAEGPEVETDYFNFTALGIPPGHPARSMQDT
                     FFLDEGLSLRTHTSAVQIRTMLVQGPPVYIVCPGRVYRRDSDPTHTPMFHQVEGLAVD
                     KGLTLGHLKGTLAAMARRVFGDDVEIRLRPSYFQYTEPSVELDVSCFVCGGSDPGCKV
                     CKGAGWLEMLGAGMVDPGVLEEVGYDPETYTGYAFGMGPDRMAMVKYGVPDLRLFFEG
                     DLRFLRQF"
     gene            2448..4880
                     /gene="pheT"
                     /locus_tag="VFQ10_03865"
     CDS             2448..4880
                     /gene="pheT"
                     /locus_tag="VFQ10_03865"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET9964509.1"
                     /translation="MRVPLSWLQEYLDFDLSADELVDLFSLRSQEIDGVHHLGVIDGE
                     VVVGEVIEFGPHPNADRLSVAKVDLGGREVQIVAGAPNPYPGARVPVVLPGSVMSDGT
                     RLGEAKLRGLQSYGMMMSERELGISQDHEGILLLDDSYEVGRPVADYFPVGDTVLDID
                     VTPNRPDLWGMIGVARELAAILGTDYRIPTPSFQGGGKPTHDFGLRVEAEDLCPRYDL
                     RRISDLQPGQEAPLWMRRRIYAAGMRPINAIVDATNYVMLETGQPIHAFDADRVRGGI
                     VVRRGRAGEKIKLLDGSTRSLDEETLVIADEERGLVIAGVMGAADAEVGEETTDALIE
                     VATFEGRNILQTSQKLGLRTDASGRFERGLDPNMVAYAMDRVSGLLAQAPGGTPADDT
                     LSHYPEPVEPWRVPLRLGRAELLLGMPVQEDEAAVRLEALGCEVEREDGRLWATVPTF
                     RRDLRREADLVEEVGRLIGLDRVPERLPAVPQAGALTAAQKKSRGLRRLLADLGLSEA
                     ITYPFGPERWTDDLGLDDGAVSLENPLSAEGAYLRTSMLPGLLDATARNRAFGARGGA
                     LFELGSVFFADVPQDDLREAALRFRMTGEHDVKDTGGLMGVREAGRIGVVLAGTVRPA
                     SWNVPEVEAGFFEAKGLVERLVPGARFVPAARPYLHPGRAAMVLVGEEEAGWVGELHP
                     EVAERFELHGWPVAALELDPALCHPDPGPRFEPFVNIPTVARDLAVLVEERVPVGDML
                     RLVQDSPSPLLTEVRVFDVYTGSPVPEGRKSVALGFTFQGEKTLTDEEVDAEVGRILS
                     MLEDEFDARVRS"
     gene            complement(4870..5427)
                     /locus_tag="VFQ10_03870"
     CDS             complement(4870..5427)
                     /locus_tag="VFQ10_03870"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9964510.1"
                     /translation="MRLDDRPRELEGERIELRRHARENYHLYAEWYGDPEIWHLTSWA
                     PSPLSRSAVERLFEDRELSKTDDSFAIHVRGDDKPIGVISLMNKSEANESAELSVIVG
                     HQDDRHQGYGTEAIDRLLSYAFEELGLNRIGLSVFDFNEEAISAYEKLGFVVEGRFRQ
                     AIKRRSGFHDAILMSILKSEWEPSS"
     gene            5702..7774
                     /locus_tag="VFQ10_03875"
     CDS             5702..7774
                     /locus_tag="VFQ10_03875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET9964511.1"
                     /translation="MPKPVGHSQRYMPGLDGLRAIAVLAVIAYHLNPAWAPGGLLGVG
                     VFFTLSGYLITDLLLGQRAATGRLQLGDFWLRRARRLLPALFLILAVVVAWVTLLDRS
                     LLPGLRGDVLAAVGYVSNWWNIVREASYFARFGPPPPLDHLWSLAVEEQFYLIWPWLL
                     WLGLRYVPGRYTLACLTLVGAALSAAAMALIYQPGVDPTRVYEGTDTRAFGLLIGAAL
                     AMVWPSRKLRADRINHRARLLIDGAGVVGLVVIGILIWRTSEYSPFLYKGGIVLLSVA
                     TVLVLTALVHPASWLGVAVGWTPLRWLGVRSYGIYLWHYPIIVLTAPGPQQGATLSLE
                     VLQVVATIVVAALSWQFFEEPIRRGAIGRIWAQGRSRLARRGDTPRGSYLPRRPFLSG
                     MVSVVAVAFLALDCAGLTGMVSPRAQTSGLSARSEKGQVHPNVSTHHMAASPTTTPTP
                     EATLSDVAVLTSTQTRESQKPKSREPESRKAESPVMSSCNSVAYIGDSTSEGMVLPNY
                     LPDPGHRLGAQYARVGATEQYLEISGARSIVETLSEKQASGYAIARDLKANGFRGCWV
                     IALGSNDTANVYVGSMVGLEARIRRMMRVMGDQPVLWITVKSLLDTGPYAENNMRRWN
                     RTVVDACAKYPNMRVFDWASLAEDSWYISDGTHYTSEGYRHRARLSADALAHAFPASG
                     ESSDCVVQ"
     gene            7859..8218
                     /locus_tag="VFQ10_03880"
     CDS             7859..8218
                     /locus_tag="VFQ10_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964512.1"
                     /translation="MKVIADRIVTLGFGKYVRADNIVALVPIEEDRGSGRRTLVYVDG
                     LPEPLVAGRTEGTILRDMVGAESGATELLRELQLQIGQVRPLLKSSIRSEAGLDLDEL
                     SRRITDLTGEQASPKMF"
     gene            8215..8796
                     /locus_tag="VFQ10_03885"
     CDS             8215..8796
                     /locus_tag="VFQ10_03885"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET9964513.1"
                     /translation="MKAAFYDRDPRQVAVALLGCILSHDTQEGKTVGVIVETEAYRPD
                     DPACHAYKGPTMRNRNIFGPPGIAYVYLSYGTHKLLNVVCEPEGVGSAVLIRALRPLD
                     GEDLMERRRGRARDLCNGPGRLTQALGVDLDYDGQDITSGSLIISDGVAPPEGIMETT
                     RIGITRGTDLPWRYLIDGDEHVSVPPKTISRRR"
     gene            complement(8777..11056)
                     /locus_tag="VFQ10_03890"
     CDS             complement(8777..11056)
                     /locus_tag="VFQ10_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET9964514.1"
                     /translation="MARTYQKRTRRGERARDIRRPVRLPERRKGSSRRRSLASRILRG
                     MGVFLLCMLVAGAAFAAGGYLGLIESVQKLDEPRTYETHPTYIYSAPVGGNEDSRRVI
                     GTIIEGQNRKMASLSEMPPHLLDAVVAKEDERFRAHGGVDLWGIMRALWVDIRAGKAV
                     EGASTITQQYVRNAYLSQDRSITRKIKEALIAIEVERKQDSKDQIIADYLNTVYFGNN
                     AYGVEAAAETYFNKSVEDLSIAESATLVGLLWSPSILGEDRDGAQYQRDLVLTKMFDT
                     GYVPSRDYNQALEEPLPEAWPANTPTKSGFTGPELTRDFAKYTHDELIRRYGVHTVLE
                     GDLSVYTTIDLKKQVAAHDIVYGPSGYLPAPDDPDLALVSIEPGTGRIETMVGDRDEG
                     SQFNLVTQGRRQPGSSFKPFALIAALEQGIDPNTRYVSENKEYKVDVGLDKPETWEVK
                     NYDGIVRGEISLKEALWRSDNTVFADLAMNAGGRGLKNGPQKIADVARRCGITTHLPE
                     HPKPSIVLGAYEVSPLDMASAYATIANDGRRVEPTAVSKVVSNEDDKDEKVLYVAPEH
                     PEGEQVISEDIANEATEIMIGDVTEGIAKDAYLGDRPVAGKTGTSENFFDAWFIGFTP
                     ELVTDTWMGYAEGGQTLEYVLEYARRLHGLSGGITPAEIWKAYTRSVLSGEPIEQFDG
                     VKMPERADPKPRLKNDPDAVRKRASASPGARGRAPESRRGAAAGASASSIAPRHSRAQ
                     RAGAWRYTAAPSSASAASR"
     gene            11124..>11590
                     /gene="tyrS"
                     /locus_tag="VFQ10_03895"
     CDS             11124..>11590
                     /gene="tyrS"
                     /locus_tag="VFQ10_03895"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET9964515.1"
                     /translation="MKAFRDHSTIYRNAVDVIPREELDRRLASGETLRVKLGIDPTAP
                     DIHLGFAVVLGKLRAFQDLGHTAVLVIGDYTARVGDPSGRSKSRPILSPEQIEENTRT
                     YLEQAYVILDRDRTEVRHNSEWLAPLRMADLIELTRATTLARILERDDFNKRYA"
BASE COUNT         2048 a         3588 c         3724 g         2230 t
ORIGIN      
        1 cggaagcaga agcggcgtct caatacagag accgacctta cccgctcgga cgagaagaag
       61 atcaagcgcc ttctggggga gagatagcat ggcacgtacc gcgcgcagcg tccacgcccg
      121 caaaaagcgc cgcaaggttc tggaacaggc gaaaggctac cgcgggacca agcacagctc
      181 gtacaagcgg gccaaggagc aggtctggaa gagcggcgtc tacgcctacg agggacgcaa
      241 gcaacgcaag agggacttca gggccctctg gatccaacgc atcaacgccg gcgcccgcga
      301 acacgatctg tcttactcca ggctgatcca cgggctgagg ctcgccgaga tagacctcga
      361 ccgcaagatc ctggccgacc tcgccgtcac cgagcccgag atcttcggcg ccatcgtggc
      421 ccaggcgaag aatgccctgg acggaaaacc ggtcgaccgg cgggtcgagg tggagcgaag
      481 cgccgagccg gaacagccta tctcatccgc cgcccgcccg cccgaagcgc ctgcagagca
      541 ggcggaagag gccgaaacct cggaggacgg actccaggcc accgatgccg cgagtcgcaa
      601 ggccgaggaa ctgaacgtga acctctccag tgtggaagga accggccagg gcgggcgcat
      661 caccgtcggc gatgtcaaga aggccgccag gggagtcgag tagccaaggg atgccgggta
      721 ccccggcgat ctccccgaaa cgggctgcga ggctcaagac caagaaacac cgcgaggccg
      781 aacgcctgtt ccttgctgag ggcgctgctg ttctcgaaga agcccctaca gcacctttac
      841 aggtcttcga tgaagtgggc cagttgcgtc gcgtttcgac gctcaagacc ccgtccggac
      901 cggtcggggt cttccctttc ctggacgtaa ccgcaaccga gctgctggcc tctgtgcggc
      961 gcgttgtgtt gttgcacggg gttcaggacc ccgggaacgt cggcacggcc atccgctcgg
     1021 cccacgcgtt cggcgccggg gtcgccctct ccagcggctc cgccgatctc tacaatccaa
     1081 agacggtcag ggcttcgatg gggtccatct tccacgcccc tgtggcgcgg gaactcgagt
     1141 ccgtcgactt cctggccgaa gcaggctccg ccggtttcga actcgtcgcg gctgtccccg
     1201 aggcaggggt cacgcccagg tctctgtccc gtggcaggct cgtcgtctgt atcggcgctg
     1261 agggctcagg gctgcctgaa gaagtcgtca gaacctgcga gatgagggtc ggtataccgt
     1321 ctctggcgcc ctcgctgaac gcgtctgtgg cggcgtcgat cctgctctac gaggcgtatt
     1381 cgcgtgtgct accataatcg cccactatga cggtgagcga gcgcatcgaa cagttgaagt
     1441 ccgaggcact ctcggccgtc tcctccagtg cttccacagc cgatctcgag gacgttcgcg
     1501 tacgatttct cggtcgctcc gcggagctaa cggagataaa gaaggccata ggcaccctat
     1561 ctcccgagga gcgcaaggag gtcggaaggt cctccaatct cgcctcgcgt gagatcgaag
     1621 cagccctcgg ctccaggacc gaagaggtcg cggcgcgtga acaggaggag cgtctgcggg
     1681 ctgaagctgt cgacgttacg ctgcctggtg tcccgttccc caggggtcac ctgcatccat
     1741 cccagatagt aatagacgac gtcgtcgact tcttcgtcgg cctgggctat cgcgtcgccg
     1801 aaggtcccga ggtcgagacc gactacttca acttcacggc cttggggata ccgccggggc
     1861 atcccgcacg cagcatgcag gataccttct ttctggatga aggtctctcc ttgaggaccc
     1921 acacctcggc cgtacagatc aggacgatgc ttgttcaggg acctcccgtc tacatcgttt
     1981 gtccgggacg cgtatatcgt cgggactccg acccgacgca cacgccgatg tttcaccagg
     2041 tcgagggcct tgcggtggac aagggcctca cactcgggca cctgaagggc acgctcgccg
     2101 cgatggcccg tcgagtcttc ggggatgacg tcgagatccg gctccgtccc agctacttcc
     2161 agtacaccga acctagcgtc gagttagacg tgagttgttt tgtgtgcggc gggtcagatc
     2221 ccggttgcaa ggtctgcaaa ggtgctgggt ggctcgagat gctgggggcc gggatggtgg
     2281 accctggcgt gctcgaggag gtgggatacg accccgaaac gtacaccggc tacgccttcg
     2341 ggatgggacc cgacaggatg gcgatggtca agtatggtgt ccctgatttg cgtctgttct
     2401 tcgagggcga cctccgcttt ctgcgtcagt tctagaaggg gttcaccttg cgcgttccgt
     2461 taagctggct acaggagtac ctcgacttcg acctctccgc cgatgagctc gtagatctct
     2521 tttccctccg aagccaggag atcgacggcg tccaccacct cggtgtgatc gacggcgagg
     2581 tagtcgtggg cgaggtaatc gagttcggac cgcatcccaa cgccgacagg ctctctgttg
     2641 cgaaggtcga tctcgggggc agggaggtac agatcgtggc cggcgccccg aacccatacc
     2701 ccggcgcccg cgtgcccgtc gtacttccag gtagcgttat gtcggacggg acgaggcttg
     2761 gagaggcgaa gctgcgcggc cttcagtcct acgggatgat gatgagcgag cgcgagctcg
     2821 gaatctcgca ggatcacgaa gggatactgc tgctcgacga ttcctacgag gtcgggcgcc
     2881 ctgtcgccga ctactttccc gttggggata ccgtcctcga tatcgacgtt actccgaaca
     2941 ggcccgacct ttggggtatg atcggggtgg ccagagagct ggcagccata cttgggacgg
     3001 actacaggat tcccacacca tctttccaag ggggagggaa gccgacccac gacttcggac
     3061 tcagggtcga ggccgaggat ctgtgtccgc gatacgatct caggcgcatc tctgacctcc
     3121 aaccggggca agaggcgccg ctgtggatgc ggcgcaggat ctacgctgcc ggcatgcggc
     3181 ccataaacgc cattgtggac gcgaccaact acgtcatgct ggagacgggc cagcccatcc
     3241 atgccttcga cgccgacagg gtgcgtgggg gtatcgtcgt ccgtcgcggg agggcgggcg
     3301 agaagatcaa acttctcgat ggttccacgc gctcgctgga cgaggagaca ctcgtgatcg
     3361 ccgacgagga gcgcgggctg gtgatcgccg gggtgatggg cgccgcggac gctgaggtgg
     3421 gggaggagac cacggacgcc ctgatcgagg tagccacctt cgaagggcgc aacatccttc
     3481 agacttccca gaagctcggc ctgcgcaccg acgcttctgg ccgcttcgag cgcggcctcg
     3541 acccgaacat ggtggcgtac gcgatggatc gggtgagtgg cctgctcgct caggctccgg
     3601 gcggaacgcc cgccgatgac accctcagcc actaccccga gcccgtggag ccctggcgtg
     3661 taccgctgcg gctcggccgg gccgagctcc tgcttggcat gccagtgcag gaggatgagg
     3721 cggcagtacg tctggaggcc ctggggtgcg aggtcgagcg ggaagatgga aggttgtggg
     3781 ctaccgttcc caccttcagg agggatctcc gccgcgaggc agacctcgtc gaggaggtcg
     3841 gacggctcat cgggctcgac agggtgccag agaggctgcc cgctgttccg caagccgggg
     3901 cgcttacggc ggcgcagaaa aagtcgcgcg ggctcaggcg tctgctcgcg gaccttggac
     3961 tctcggaggc tatcacctac ccgttcggcc ccgaacgctg gacggatgac ctcggcctgg
     4021 acgatggggc ggtcagccta gagaaccccc ttagtgctga gggcgcctat ctgcgaacca
     4081 gcatgcttcc ggggctgctc gacgccacgg cccgcaacag ggctttcggg gcgcgaggcg
     4141 gggcgctgtt cgagctaggg agcgtgttct tcgcagacgt cccccaggac gacctgcgag
     4201 aggccgcgct gcggttccgc atgacgggcg agcacgatgt gaaagacacc ggcgggctca
     4261 tgggcgtccg tgaggccggc aggatcggcg tcgtgctcgc cggcaccgtg cgccctgcta
     4321 gctggaacgt tcccgaggtc gaagccggtt tcttcgaggc gaagggtctg gtcgagcggc
     4381 tggtgcctgg cgctcgtttc gtacccgcgg cgagaccgta tctgcatccc ggacgcgcgg
     4441 cgatggtgct ggtcggcgag gaagaggctg gttgggtcgg ggaactacat cccgaggtag
     4501 cagagaggtt cgagttgcac ggctggcctg tcgctgctct tgagttggat cccgccctgt
     4561 gccatccgga tcctgggccg cgcttcgaac ctttcgtcaa tattccgacc gtcgccaggg
     4621 atctggccgt cctcgtcgaa gagcgggtac cggtaggcga catgttgcgc ctggtacagg
     4681 actcgccgag ccccttgctg accgaggtgc gcgtcttcga cgtctacacg ggaagtcccg
     4741 tcccagaagg gcgaaagagt gtggccttag gtttcacgtt ccagggtgag aagaccctca
     4801 cggacgaaga ggtggacgct gaggtcgggc gtatcctcag catgctcgaa gacgagttcg
     4861 acgcacgcgt caggagctag gctcccactc cgacttcagg atgctcatga ggatggcgtc
     4921 gtgaaagccg gagcggcgct ttatggcctg gcggaaacgc ccctctacca cgaagcccaa
     4981 cttctcgtag gccgagatgg cctcctcgtt gaaatcgaag acgctaagac cgatgcggtt
     5041 gagtccaagc tcctcgaagg cgtagcttaa cagccggtcg atggcctctg tcccataacc
     5101 ttggtggcgg tcgtcctgat gaccaactat aacggagagc tccgccgact cgttggcctc
     5161 gctcttgttc atgaggctga tcacacctat gggcttgtca tcgcccctga cgtggattgc
     5221 gaaagaatcg tctgtcttag agagctccct gtcctcgaac aacctctcga ccgccgaacg
     5281 gctcagaggc gatggcgccc aactcgtcag gtgccaaatc tccgggtccc cgtaccactc
     5341 cgcgtagagg tggtagttct cgcgggcgtg gcgccgaagc tcgatcctct caccttccag
     5401 ctctctgggt ctgtcgtcaa gccgcacggc caggaatata ccgggtttgg cggggatgcg
     5461 ccacgctaca ttttgtgctt cagaacaggt ttctctgggc acattgccaa cctcgcggag
     5521 atcgcgggta caatggggcc catacggagc cgtgtagtag agtggagggg ccggagccgt
     5581 tgcttactcc tttagcagga atcggatcgt cgactcgtaa tgacctactg cccaggtagg
     5641 acggtcggcg gggtaacggt gagtctcggc ctgtactatg ttggcgctgg gggagggcat
     5701 catgcctaag ccggtcgggc attcccagcg ctacatgcca gggctcgacg gactgcgggc
     5761 catcgcggtg ctcgccgtga tcgcgtacca cctgaatcca gcctgggcgc caggggggct
     5821 gctaggggtt ggtgtgttct ttaccctgag cggctacctg ataacggatc tgctccttgg
     5881 ccagcgagcg gctacggggc gccttcagct cggtgacttc tggctccgca gggcgcggcg
     5941 gctcctgccc gcccttttcc tgatacttgc ggtggtagtg gcgtgggtta cgttgctgga
     6001 tcggtctctt ctcccgggcc tcaggggaga cgtgctggcc gccgtcggct acgtgagcaa
     6061 ctggtggaac atcgtccggg aggcatccta cttcgcccgc ttcgggccac cgccgccact
     6121 agatcacctg tggtccctgg ctgtggagga gcagttctat ctgatctggc cctggttgct
     6181 ctggctcggg ctgcgctacg tccctgggcg ctacaccctg gcctgcctga ctctggtggg
     6241 cgcagccctc tccgccgccg ccatggccct gatctaccag ccaggcgtcg acccgacgcg
     6301 ggtctacgaa ggaaccgaca cccgcgcctt cggcctcctc atcggtgccg cgctggccat
     6361 ggtgtggccg agccgcaagc ttcgcgccga ccgtatcaac catcgagccc gcctcttgat
     6421 tgacggtgcg ggagtcgtcg ggctggtcgt gatagggatc ttgatctggc ggacgagcga
     6481 gtactcgccg tttctgtaca aaggtgggat cgtgctcctt tcggtggcga cggtcctggt
     6541 cttgaccgcc ctcgtacatc ccgcaagctg gctaggcgtg gctgttggct ggacgccgct
     6601 tcgctggctc ggtgtgcgct cctacggcat ctacctgtgg cactacccga tcatcgtgct
     6661 caccgcgccg ggcccgcagc agggggcaac cctttccctc gaagtgctcc aggtggttgc
     6721 gacaatcgtc gttgccgccc tctcctggca gtttttcgag gaacccatcc gccgcggtgc
     6781 cataggacgg atctgggcgc agggccgatc aaggctggcg cgcagagggg acaccccgcg
     6841 aggaagttat cttccgcgga ggccatttct cagcgggatg gtctctgttg tagcggtagc
     6901 cttccttgcg ttggactgcg caggcctcac cgggatggtc agcccacgcg cacagaccag
     6961 tgggctttcc gctcgctccg aaaaggggca agtgcatccc aatgtctcca cacatcacat
     7021 ggccgcttct ccaaccacga ctcctacccc agaggctacg ttatcggatg ttgcggtact
     7081 tacctctact cagacacgag agtcacaaaa gccgaaatca cgagagccgg agtcgcgaaa
     7141 ggctgagtcg cccgtcatgt cctcttgcaa ctcggttgct tacatcggcg actctacgtc
     7201 ggaaggcatg gtcttgccca actatttgcc cgatcccggc caccgcctgg gagcgcagta
     7261 cgcgcgggtc ggcgctacgg agcaatacct cgagatctcg ggtgccagat cgatcgtcga
     7321 gacgctatct gagaagcagg ccagcggcta cgcgatagcc cgtgatctga aagccaatgg
     7381 cttccgggga tgctgggtga tcgcgctcgg tagcaacgac acagctaacg tctacgtggg
     7441 ttctatggtg gggctggagg ccaggatcag gaggatgatg cgggtgatgg gcgaccagcc
     7501 ggtgttgtgg atcaccgtga agtcgcttct ggatactgga ccctacgcgg agaacaacat
     7561 gcggcgctgg aaccgcaccg ttgtagacgc ctgcgcaaag tacccgaaca tgcgggtctt
     7621 cgactgggct tcgctggcgg aagatagctg gtatatctcg gacgggaccc actacacttc
     7681 ggagggctac aggcaccgcg cccgtctgtc tgcggacgcg ctggcccacg cattccccgc
     7741 ctcgggagag agttcggatt gcgtcgtcca gtagcctgtg gcggtctttg cggcgtgacg
     7801 ggtcgcccga ggccggcggg gatgatataa gggtctgtag gatggagatt gtatcgagat
     7861 gaaggttata gcggaccgta tcgtcaccct cgggttcgga aaatacgtaa gggccgataa
     7921 catcgttgcc ctcgtcccga tagaagagga ccgcggaagc ggacgccgca cgctcgtcta
     7981 cgtcgacggc ctgcccgagc cactcgtcgc cggaaggacg gagggcacga tcctcaggga
     8041 catggtcggc gccgagagcg gggcgacaga gctactccgc gagttgcaac tccagatcgg
     8101 gcaggtccgt ccccttttga aatcttccat tcgctccgag gccgggctcg acctcgacga
     8161 gctctcccgc cgcataacag accttacggg ggagcaggca tctcccaaga tgttctgaag
     8221 gccgctttct acgacaggga tcccaggcag gtcgcggtag ccctcctcgg ctgcatcctc
     8281 tcccatgaca cccaggaggg caaaacggtg ggcgtgatcg tcgagacaga agcgtaccgt
     8341 cccgatgacc ccgcttgcca cgcttacaaa gggccgacga tgcgcaaccg taacatcttc
     8401 ggcccgccag gcatcgccta cgtctacctc tcctacggta cgcacaaact actcaacgta
     8461 gtctgcgagc ccgagggcgt cgggagcgcc gttttgatca gggcgctacg ccccctcgac
     8521 ggcgaagatc tgatggagcg gcgtaggggc cgggcgcgtg acctttgcaa cggccctggc
     8581 cgcctgaccc aggccctcgg cgtggacctg gattacgatg ggcaggacat cacctcaggt
     8641 agcctgatta tctcggacgg agtagcgccg cctgagggca tcatggagac cacccggatc
     8701 ggcataacac gcggtaccga ccttccgtgg cgttacctca tcgacggaga tgaacacgtc
     8761 tccgtcccac cgaagactat ctcgaggcgg cgctagcact ggaaggtgca gccgtgtaac
     8821 gccaggctcc ggctcgttgg gcccgagaat gtcttggggc gatgctggat gccgaggcgc
     8881 ccgccgccgc tccccgacgg gattctgggg cccgcccgcg ggctcccgga ctcgcagagg
     8941 cgcgtttacg gacagcatcg gggtcgttct tcaagcgggg tttggggtct gctctttcag
     9001 gcatcttgac gccgtcgaac tgttcgatgg gttcaccgct caagactgat cgcgtgtacg
     9061 ccttccagat ctcggccggg gtgatgccgc ctgagaggcc atgtagtctg cgggcgtatt
     9121 cgaggacgta ttcgagagtc tgcccgcctt cggcgtagcc catccacgtg tcagtaacca
     9181 gttcgggagt gaagcctata aaccaggcgt cgaagaagtt ctcgctcgta cccgtcttgc
     9241 cagcgaccgg gcggtctccc aggtaggcgt ccttcgctat accttctgtg acatctccta
     9301 tcatgatctc ggtcgcctcg ttagctatgt cctcggaaat tacctgctcg ccctcaggat
     9361 gctcgggtgc cacatagagc accttctcat ccttgtcgtc ctcgttgctt accaccttcg
     9421 aaaccgcggt cggttcgacc ctgcgtccgt cgttcgcaat cgtcgcgtag gccgatgcca
     9481 tgtcgagtgg agaaacctcg tacgcgccga gtactatgga aggcttggga tgttcgggga
     9541 gatgcgtggt gatcccgcat ctccttgcga cgtccgcgat cttctgtggt ccgtttttca
     9601 ggccacggcc gccagcgttc atcgccaggt ctgcgaacac cgtgttatcc gacctccaga
     9661 gcgcctcttt aaggctgatc tcaccccgca cgatgccgtc gtagttcttt acctcccacg
     9721 tctccggttt gtcgaggccg acgtcgacct tgtactcctt attctccgag acgtacctgg
     9781 tattgggatc gatgccttgc tccagggctg cgatgagggc gaacggcttg aacgagctgc
     9841 cgggctggcg cctgccctgg gtgacaaggt tgaactggga gccctcgtcc ctgtcgccga
     9901 ccatagtctc tatacggccg gtacctggct ctatggagac gagggcgagg tcgggatcgt
     9961 caggcgcggg gaggtatcca gaaggtccgt agacaatatc gtgggcggcg acctgcttct
    10021 tcaggtctat ggtggtgtag acgctgaggt ctccttctag aacggtatgc acgccgtaac
    10081 gccgaatgag ctcgtcatgc gtgtacttgg cgaagtcgcg ggtgagctca ggccctgtga
    10141 accccgactt ggtcggtgtg ttggcgggcc aggcctcagg cagtggctcc tcgagggcct
    10201 ggttgtagtc ccggctcgga acatagcctg tgtcgaacat cttggtcagt acgaggtctc
    10261 gctgatactg tgccccatcg cggtcctctc cgaggataga cggagaccac aggagcccga
    10321 caagggtcgc cgactccgcg atgctcagat cctcgaccga cttgttgaag tatgtttcgg
    10381 cggccgcctc caccccgtag gcgttgttac cgaagtagac ggtattcagg tagtcggcga
    10441 tgatctggtc cttcgagtcc tgtttgcgct caacctctat ggcgatgaga gcttctttga
    10501 tcttcctggt gatcgaacgg tcctgcgaga ggtaggcatt cctgacatac tgctgggtga
    10561 tggtgctggc accctcgacc gcctttccgg ccctgatgtc gacccagaga gcgcgcatga
    10621 tcccccagag gtcgacgccg ccgtgcgccc tgaagcgctc gtcttctttg gcgaccacgg
    10681 cgtcgagcag gtgcggcggc atctccgata gcgaagccat cttgcggttc tggccttcga
    10741 ttatcgtgcc gatcacacgc cgcgagtcct cgttgccgcc gaccggcgcc gagtagatgt
    10801 aggtgggatg ggtctcgtaa gtccgcggct cgtccagctt ctgcacgctc tctatgagcc
    10861 ccaggtaccc gcccgccgcg aacgccgccc cagcgaccag catgcacaac aaaaagaccc
    10921 ccatgccacg cagaatcctc gaggccaggc tcctcctgcg ggaagatccc ttgcggcgct
    10981 cgggtagacg taccggccgt cggatgtccc tggcccgctc accccgcctc gttctcttct
    11041 ggtaggtgcg ggccatcgag ccggtatact acactaaggt ggggcaggtt acatttgtat
    11101 tacgaggaag ttgcaatctg ttaatgaagg cctttcgaga ccacagcacg atttacagga
    11161 acgccgtaga tgtgatccca cgggaggagc tagaccgccg cctcgctagt ggcgagactt
    11221 tgcgcgtaaa gctgggtata gaccccacgg cccctgacat ccacctcggc ttcgctgtgg
    11281 tcctagggaa gcttcgtgca ttccaggatc tggggcacac cgctgtgctc gtgatagggg
    11341 actacacggc gcgcgtcggc gacccatccg ggcgttcgaa gtcgcgtccg atactctcgc
    11401 cggagcagat cgaggagaac acgcggacct acctcgagca ggcgtatgta atcctcgacc
    11461 gcgatcggac cgaggtccgc cataactcag agtggctcgc gccgctgagg atggcggatc
    11521 tcatagagct gaccagggcg acgacattgg cccgcatcct ggagcgcgac gacttcaaca
    11581 agcgctacgc
//
LOCUS       DASTKM010000096         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_373733, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000096 DASTKM010000000
VERSION     DASTKM010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373733"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1545
                     /locus_tag="VFQ10_03900"
     CDS             211..1545
                     /locus_tag="VFQ10_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET9964516.1"
                     /translation="MRPVKSFLSHDGSWAYRSMLLISFCTVALGVWARLYHLGFPPRI
                     LWDEKYFPAMANKYLQGVYQFDLHPPLGKFIIAAGISLLGNEALGWRIMPTLFGLALL
                     PLAAVLGWYCLKERVGAMLMATFFAAETIFIVHSRTGVMDIFVVFFVLAAILAALRAE
                     SDRQVIFSSVLLGMAIAVKWAAFPVAVPIGYVLWRKGLLRPFLGGLWVAAVVYVAVVY
                     VGALITITANPIEAWVWAWDWHVDAARKVTAVIPHTWGSPWWSWPIMLRPIRFFYGAN
                     AEGLLQVIVAIGNPLIWWSSTLAVVVSILDIARKAVARKLSADDPIIPIVLGYVFLLA
                     PWVAGTRIPYIYNYLPIYPFAILALVYWLCKIWNRLRWGPWVVVAFTACAVALTVFFL
                     PLVLAIPISEDNLMRRVWIDYWFEYEKPVPGSGCLVPNPTRLCPPVNATLSR"
     gene            complement(1529..1987)
                     /locus_tag="VFQ10_03905"
     CDS             complement(1529..1987)
                     /locus_tag="VFQ10_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET9964517.1"
                     /translation="MNGVVEGLYVTGEGSAAMERVEEVRTVEGCGIEGDRYCEGTGFW
                     TRYGDVCEVTLIEGEDLDYIENELGISVKNGEHRRNIITRGIRLGDLRRKKFHIGETV
                     LEYDRPRPPCRHVQDLSQPGMRRALKGRGGICARVVRAGSIRVQDAIETE"
     gene            complement(2034..>2658)
                     /locus_tag="VFQ10_03910"
     CDS             complement(2034..>2658)
                     /locus_tag="VFQ10_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET9964518.1"
                     /translation="APLTIEAPRGSLVNAAYPAAVVAGNVETSNRIADTVLAALAGFA
                     PERIPAQGQGTMNNTVIGGQGWTYYETIGGGQGASNRGPGPSGVHVGMSNTLNTPIEA
                     FEMEYPMRVERYELLYDSGGDGEHRGGDGIVRAVRVLEPASLSLLTDRRRHGPRGVDG
                     GEPGRVGRNLLGGEELPPKVSREMEEGDVVAVETPGGGGYGRAGGGT"
BASE COUNT          419 a          896 c          781 g          562 t
ORIGIN      
        1 tgtttccgtg gtgaaactgc gagaggagat caccgatccc gacacagagc gggcgaatat
       61 ctacgaggag cactacgaaa tctaccgctc gctctacccg gcgacgagta ctgccatgtc
      121 ccgcctcacg gacctcgccg ctgattccac cggcgagtaa tctagtactg aagcgattct
      181 cacttacaaa ccttcgcgaa acctagtaac gtgcgtcctg tgaagtcctt cctgagccac
      241 gacggatcgt gggcctaccg ctcgatgctg ctcatcagct tctgcacggt cgcgcttggg
      301 gtgtgggcca gactgtacca tcttgggttc ccacccagga tcctgtggga cgagaagtac
      361 ttcccggcga tggccaataa gtacttacag ggcgtctacc agttcgacct tcatccgccc
      421 ttgggaaagt tcatcatagc agccggcatc tccctcctgg gcaacgaggc ccttgggtgg
      481 aggatcatgc ctacattatt cggcctggct ctcttgccgc tcgcggcagt cctcggctgg
      541 tattgcctca aggagagggt tggcgccatg ctgatggcca ccttcttcgc cgccgagacg
      601 atattcatag tccattccag gaccggggtc atggacatct tcgttgtttt cttcgtgctc
      661 gcggccattc ttgcggcgct gcgagccgag agtgacaggc aggtgatctt ttcttccgta
      721 ctgctcggca tggccattgc cgtcaagtgg gcagccttcc ctgtcgccgt gcccattggc
      781 tatgtcctct ggcgcaaggg actgcttcga ccatttctgg gggggctctg ggtcgccgct
      841 gtcgtctacg tcgccgtggt ctacgtcggg gcgctcatca ccatcactgc gaaccccatc
      901 gaagcatggg tgtgggcttg ggattggcac gtcgatgccg cgcgcaaggt caccgccgtc
      961 atcccccaca cctggggctc gccctggtgg agctggccga tcatgctgcg gcccatacgc
     1021 tttttctacg gggcgaacgc ggagggattg ctacaggtaa tcgtcgcgat cggcaacccc
     1081 ctgatctggt ggagcagcac gctggccgtg gtcgtgagca tcctcgacat agcgcgcaaa
     1141 gccgtagcca ggaagctctc ggcggacgac ccgatcatac cgatagtgct cggctacgtg
     1201 ttcctcctcg cgccctgggt ggccggcacc aggatcccgt acatctacaa ttacctgccc
     1261 atctacccgt tcgccatcct ggcgctggtc tactggctct gcaagatctg gaatcgactg
     1321 cggtgggggc cctgggtggt cgtcgccttt acggcctgcg ccgtggccct gaccgtgttc
     1381 ttcctcccgc tggtcctggc catacccata agcgaggaca acctcatgcg ccgcgtatgg
     1441 atcgactact ggttcgaata cgagaaacca gttcctggca gcggatgtct cgtcccgaac
     1501 cccaccaggc tttgcccccc agtgaacgct actctgtctc gatagcgtcc tgtaccctga
     1561 tgctgccggc ccggacgact ctggcgcaga tgccgcctcg acccttgagg gcccgcctca
     1621 tccctggctg gctcaggtcc tgtacgtgcc tgcagggcgg acgtgggcgg tcgtactcca
     1681 gcacggtttc cccgatgtgg aacttcttcc tgcgcaggtc gcccagcctt atgccgcggg
     1741 tgattatgtt gcgcctgtgc tcgccgttct tgacgctgat gcccagctcg ttctcgatgt
     1801 agtcgaggtc ttcgccctcg atcagggtca cctcacaaac gtctccgtaa cgcgtccaga
     1861 atcctgtccc ctcacagtag cggtcccctt cgatgccaca cccctcgacg gtacggacct
     1921 cctccacgcg ctccatggcc gcgctgcctt ctcctgtaac gtagagcccc tcgacgacac
     1981 cgttcatcct gcttgctccc tccggtaagt agaccacggc aagaggttag cagctacgtg
     2041 cccccgccgg cacgcccata accgccaccg ccgggtgtct cgacggccac gacgtctccc
     2101 tcctccatct ccctgctgac cttgggcggc aactcctcgc cgcccaggag attgcggccg
     2161 actcttccag gttcgccgcc gtcgacgccg cgcggcccgt ggcggcggcg gtcggtgagc
     2221 aacgagaggc tggcgggttc gagtacacgc acggcgcgga cgatgccatc gccgccgcgg
     2281 tgctcgccgt ccccgccaga gtcgtagagc agctcgtagc gctcgacccg catgggatac
     2341 tccatctcaa aggcctcgat aggagtgttg agggtattgc tcatcccgac gtggacgcct
     2401 gatggacctg gacccctgtt gcttgccccc tggccgccgc cgatggtttc gtagtacgtc
     2461 cagccctgac ccccgatcac ggtgttgttc atcgtaccct ggccctgcgc gggtatcctc
     2521 tctggggcga agccagcgag cgcggcgagc acggtgtcgg cgatgcggtt ggaggtctcg
     2581 acgttcccgg ccacgaccgc agcagggtag gccgcgttga ccaggctccc cctgggggcc
     2641 tctatggtga gcggggcg
//
LOCUS       DASTKM010000097         6527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1079458, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000097 DASTKM010000000
VERSION     DASTKM010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079458"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /gene="rsmI"
                     /locus_tag="VFQ10_03915"
     CDS             <1..795
                     /gene="rsmI"
                     /locus_tag="VFQ10_03915"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET9964519.1"
                     /translation="MPLTVVPTPIGNLEDITLRALRYLREVDLVACEDTRRTGRLLAH
                     YEIKKDLIAYHEYNEERLAPDLAERAKTERVALVTDAGTPLVSDPGYRLVRACIEAGV
                     EIEALPGPSALTTALVVSGLPADTVVFTGFPPRKGRERRELLERVATEPSTFVLFESP
                     HRVGKTLRDLPSGTPVAVCRELTKLHEEVFRGTAEEAARHFPGRVKGEIVLVIRGGTA
                     DEGLEEVVRRARDYVAEGESPSRAAARAARDSGHRRGEIYERLVKG"
     gene            855..1655
                     /locus_tag="VFQ10_03920"
     CDS             855..1655
                     /locus_tag="VFQ10_03920"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET9964520.1"
                     /translation="MPLADDSGEEVMGAMLVDSHTHLLRLEVSPERAVRSAAEVGVVA
                     VVNVGTDVEDSQRGAELAAVLPNVYSTAGIHPHNAGTYTAADLEALAELSESPGIVAL
                     GEVGLDYYRSQWSGEVQRALFEDVISLANDTRLPLVIHSREAFADTMACLGSARVPIV
                     LHCFEGGDREVREATERGYYVGLAGNVTYRDSATARVLRLIDEERILVETDAPYLSPQ
                     PVRGKKNAPENVVYTAGFVAERLGIEDEELATLTTRNARTLFGLPLEV"
     gene            1662..2438
                     /gene="rsmA"
                     /locus_tag="VFQ10_03925"
     CDS             1662..2438
                     /gene="rsmA"
                     /locus_tag="VFQ10_03925"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET9964521.1"
                     /translation="MSEPGFPKKRFGQHFLNDPNTARIVASGITEDDVVLEVGPGRGF
                     LTAFLADKAALVHAVELDPDVLPSLREAVGERANVRIHEGDALRLDYSGLDPAPNRLA
                     ANLPYNIASPLVLLLLEEVETLRTLRFMVQFEVARRMAAERGSKDYAAYAVLTQLLAR
                     VRILHKVPPTVFDPPPRVHSAVVEMGRREPPDDYTGIKRLVLSAFRSRRKQLVNNLPE
                     PARGPAPRLLESLGHGPNVRAEELMPENFAALYAALSWAL"
     gene            2438..3310
                     /gene="ispE"
                     /locus_tag="VFQ10_03930"
     CDS             2438..3310
                     /gene="ispE"
                     /locus_tag="VFQ10_03930"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET9964522.1"
                     /translation="MREVRLGAFAKVNYALDVLGLRVDGYHEVRTVMQSISLADELLL
                     RRADGGFDLSLEPEEVEIGPQEHNTTYLAWKALQRLTGEKLPVEVLLRKRIPAGSGLG
                     GGSADAAAVLVGLNGLFGLGLRVDELREIGACIGADVPFCISGGTAMGEGVGEILTPL
                     PVPPAHLLVVATPPRSADTGKIYHAFDEARINITHSVEPVVSALRSGSLPALAAAVGN
                     DLAPVTRDIIPEVADLEQSLLASGALGASMSGSGTAVFGAFEDAEAAGIAKDTVNAPF
                     VNVYEPVPRGVEID"
     gene            3401..3471
                     /locus_tag="VFQ10_03935"
     tRNA            3401..3471
                     /locus_tag="VFQ10_03935"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3433..3435,aa:Gln,seq:ttg)
     gene            3531..4940
                     /gene="glmU"
                     /locus_tag="VFQ10_03940"
     CDS             3531..4940
                     /gene="glmU"
                     /locus_tag="VFQ10_03940"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET9964523.1"
                     /translation="MSESSPIFAVILAAGKGTRMKSNRAKVLHTLCGVPMVNYVIEAT
                     RPLVPEGLFVVVGHQAGLVEAVLPEDAKPVLQEEQRGTGDAVRVALAALEDEVNGVLL
                     VVNGDGPLISDGTLGELVERHSSAGVGATVLVAELEDPSGLGRVVEDAGVVRIVEERD
                     ATEYERQIRLVNLGIYAFRLSEIRDTIGGLTSENQGGELYLTDVLETIGRRSRAVTYR
                     LKNPEEANLVNDRSQLARAEAILRRRILDAHMRDGVTVRDPASTHIEASVEIGRDTVI
                     LPGSLLRGETRIGTDCVIGPSTDLQDTRVGDGALVEHSVGRGAEVGDGASVGPYSFLR
                     TGTVLDPGAKVGAYCEVKNSRVGAGSKIPHLSYVGDAEIGADANLGAGTITANYDGEN
                     KHRTLVGDGVHTGVNTNLIAPVTVGEDAYTGAGSVVNKDVPAGKLAVGAPARAIRESP
                     QRRRKRAQQGDSAKKETEG"
     gene            4948..5943
                     /locus_tag="VFQ10_03945"
     CDS             4948..5943
                     /locus_tag="VFQ10_03945"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET9964524.1"
                     /translation="MMQNGYLTQTTEKRLMIVSGQGYPDLSERIAERLDIELSRVELR
                     EFPNGELYARYLESVRGADMFIVQSLCDPVNENLMQLLIMIDAAKRASAKRIVAVIPW
                     YAYSRQDRKTKPREPITARLIANMIQAAGAERVMTMDLHSGQIEGYFSFPVDHLTAMH
                     TFVDYFVDQGFQDAEDAVVVAPDTGEVKVAKQLAGHLRLPWAIVNKMRDRPGHSEVTH
                     VIGNVEGKRVVMIDDVVATGGTLVNAAKRLVEQGAIEVSAAATHGEFSGDAYRKIEDS
                     PLKEVVVTNTLPLKEDEPRSKIQTLNIAPILASTIKNVFTDESVSEVFMGENQLF"
     gene            5961..>6527
                     /locus_tag="VFQ10_03950"
     CDS             5961..>6527
                     /locus_tag="VFQ10_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET9964525.1"
                     /translation="MADNVDLQAQERDTRGKNAARRLRASGMTPAVLYGEGNEAGGST
                     ALAVPTKTVDYTLHHFGDNALYNIDFDGATSTARVMDAQRDPVSGVLLHVDFAPVDML
                     ERIEVTVPLAVVGEAPGVEEGGVLQQVAYEIQVESLPGDIPQEVTIDVSGLGMHENLT
                     LGDLTLPEGVTLLSEPEEVAVTVTAPTEI"
BASE COUNT         1256 a         1964 c         2215 g         1092 t
ORIGIN      
        1 atgcccctca cagtagtccc aacccccata ggcaacctgg aggacatcac ccttcgggcc
       61 ctcaggtacc tgcgtgaggt agacctcgtg gcctgcgagg acaccaggcg tactggacgc
      121 ctgctcgccc actacgagat aaagaaggac ctcatcgcct accacgagta caacgaggaa
      181 cgcctggccc ccgacctggc cgaaagggcg aagacggagc gcgtagccct cgtcaccgac
      241 gccggcaccc cgctcgtctc cgatcctggc tacaggctag tgcgcgcctg catcgaggcc
      301 ggcgtcgaga tagaggccct ccctggccca tcggccctca ccacggcact agtggtctcg
      361 gggctacccg ccgatacggt cgtcttcacc ggcttcccgc cgcgcaaggg aagagagcgc
      421 agggagttac tggagcgcgt cgccaccgag ccgtccacct tcgtgctctt cgagtcacca
      481 catcgggtcg ggaagacgct aagggatctt ccttccggga ccccggtcgc cgtgtgcagg
      541 gagcttacga agctccacga ggaggtcttc agggggacgg ctgaggaggc tgccaggcac
      601 ttccccggga gggtaaaggg cgagatcgtg ctcgtaatcc gcggcggaac ggcggatgag
      661 ggcctcgaag aggtagtccg ccgggcgcgg gactacgtgg cggaggggga gagtccttcg
      721 cgggcagcag ctagggcagc ccgcgactcg ggacatcgtc gcggtgagat ctacgagcgt
      781 ctggtaaagg gttgaaggag gtttctactt cagcgtggaa acgccttcca cacctgacac
      841 ccgatacctt tataatgccc ctcgctgatg actctgggga agaggtgatg ggcgctatgc
      901 tcgtggactc gcacacgcac cttttgcggc tcgaggtctc gccggagaga gcggtaagaa
      961 gcgctgcgga ggtcggagtc gtggctgttg tgaacgtcgg gaccgacgtc gaggattcgc
     1021 agaggggggc cgaacttgcg gcggtgttgc cgaatgtcta ctcgacggca ggtatacatc
     1081 cccacaacgc gggtacgtac acggcggccg acctcgaagc gctggccgag ctctcggagt
     1141 cgccggggat agtggccctt ggtgaggtgg ggctcgacta ctaccgctct cagtggtcgg
     1201 gggaggtgca gcgggcgctg ttcgaggatg tgatctccct tgccaacgat acgcgcctgc
     1261 cgttggtgat ccactcccgc gaagccttcg ctgacacgat ggcctgcctc ggaagcgcca
     1321 gggttcctat cgtcctgcac tgcttcgagg gcggcgaccg tgaggtcagg gaggcgacgg
     1381 agcgcggcta ctacgtcggc ctagcaggca acgtcacgta cagggacagc gcgacggcca
     1441 gggtgctccg cctgatagac gaggagcgca tcctcgtgga gacggacgcc ccctacctca
     1501 gtccccagcc tgtgcgcggc aagaagaacg cacccgaaaa cgtcgtatac acggccggat
     1561 tcgtcgccga gcggctcggg atagaagatg aggaactggc caccctcacc acccgtaacg
     1621 cccgcaccct cttcggcctg cctctagagg tataagctgc ggtgtccgag ccgggcttcc
     1681 cgaaaaagcg attcggacag cacttcctga acgaccccaa caccgccagg atagtggcct
     1741 cgggcataac ggaggatgac gtggtcctcg aggtcggccc gggacgggga ttcctgacgg
     1801 ctttcctcgc cgataaggcc gcgctggtac atgccgtgga gctggacccc gacgtgctgc
     1861 cgagcttgcg tgaggccgtt ggagagaggg cgaacgtgcg gattcacgaa ggagacgccc
     1921 tccgcctcga ttactccgga ctcgaccccg cgccaaacag gctggccgcc aatttgccct
     1981 acaacatcgc ctcgccgctc gtgctgctgt tgctcgagga agtggagacg ttgcggacgc
     2041 tgcgatttat ggtgcagttc gaggtggcac gcaggatggc ggcggagcgc ggttccaagg
     2101 actacgctgc atacgccgtg ctaacgcagc ttctggcgcg cgtcaggatt ttgcacaaag
     2161 tcccgccgac ggtgttcgat ccgcctccca gggtacactc cgcggtcgtc gaaatgggtc
     2221 gcagagaacc gccggatgac tacacaggga taaagcgcct ggtgctaagc gcgttcagaa
     2281 gccgccgcaa acagctcgta aacaacctgc cagagccggc ccgcgggccg gctccacggc
     2341 ttctcgagtc ccttggacat gggcctaacg tgagggctga ggagctgatg ccggaaaact
     2401 tcgcggcctt gtacgcggcg ctatcctggg cgttgtgatg cgggaggttc ggctcggggc
     2461 gttcgccaag gtcaactacg cgcttgacgt tctcgggctt cgtgtggacg ggtaccacga
     2521 ggtcaggacc gtcatgcaga gcatctcgct cgccgacgag ctgctgttac ggcgcgccga
     2581 cggagggttc gatctctcgc tggagcctga agaggtggag atcggaccgc aggaacacaa
     2641 cactacctac ctggcctgga aggcgctgca gagattgacc ggtgagaagc ttcctgtgga
     2701 ggtccttttg cgtaaaagga ttccagccgg gtctggactt ggtggcggct cggccgatgc
     2761 agcggcggtc ctcgtcgggc taaacgggct attcggcctc gggctgcggg tagatgagtt
     2821 gcgtgaaatc ggggcctgca tcggggccga tgtgcctttc tgcatctcgg gagggaccgc
     2881 catgggcgaa ggcgtcgggg agatcctcac gccgcttcct gtgccaccgg cccatctcct
     2941 cgtcgtcgcc acaccgcccc ggagcgcgga cacgggtaag atctaccacg ccttcgacga
     3001 ggcaagaata aatatcacac actccgtcga gcctgtcgtc tcggcgttgc gttcggggag
     3061 tctcccggcc ctcgccgcgg ccgtaggcaa cgacctggcc cccgtgacca gggacatcat
     3121 cccagaggta gccgacctcg agcagtcgct tctagcatca ggggcgctgg gggcttcgat
     3181 gagcggcagc gggacggcag tcttcggagc cttcgaagac gctgaagcag ccgggatcgc
     3241 gaaggatacg gtcaatgctc ctttcgtcaa cgtctacgag ccggttcctc gcggggtgga
     3301 gattgactga gcagaaagat cggacgcacg caagtgggtc ctatcaatcg aggttctgac
     3361 ggcgaaaagg cgctccggta taatcgtcgc gggcctgtac tggggcgtgg ccaagtggca
     3421 aggcaccggg ttttggtccc gggatcgcag gttcgaatcc tgccgcccca gcagatgagc
     3481 gtgtggtagg cgacagccgg gtagacgaac gataggagtg gtgctagtac atgtctgaga
     3541 gttcgccgat cttcgccgtg atcctggcgg ctggcaaggg caccaggatg aagtcgaacc
     3601 gggcgaaggt gctgcacacc ctgtgcggtg tccctatggt caactacgtg atcgaagcca
     3661 ccaggcccct cgtccccgag gggctattcg tcgttgtagg ccatcaggcc gggctcgtcg
     3721 aagcagtcct gcccgaggac gcgaagcctg ttctgcagga ggaacagcgc gggaccggtg
     3781 atgccgtgcg cgtcgccctg gccgcgctcg aagatgaagt gaacggcgtg ctcctcgtgg
     3841 tcaacggcga cgggcccctt atctctgatg ggaccctcgg agaactggtc gagcggcaca
     3901 gttccgccgg cgttggggcg acggtcctcg tggccgagct ggaggacccg agtggcctcg
     3961 ggagggtggt cgaggacgcc ggcgtggtca ggatagtcga ggagagggac gcgaccgagt
     4021 acgagcgaca gatcaggctc gtcaacctcg gcatctacgc gttcagactc tccgagataa
     4081 gggatacgat cggggggctc acctcggaga atcagggcgg cgagctgtac ctgacagacg
     4141 tactggagac gatcgggcgg cgcagccggg cggtcactta caggctcaag aatcctgagg
     4201 aggcgaacct cgtcaacgac cgctcccaac ttgcgcgggc cgaggcgatt ctcaggcgcc
     4261 gcatcctgga tgcccacatg agggacgggg tgacggtgag ggacccggct tctacgcaca
     4321 tagaggcttc ggtcgagatc gggcgcgaca ctgtgatcct gcctggaagc ctgttgcgcg
     4381 gggagacgag gatcggtacg gactgcgtga tcgggccctc cacagacctc caggacacgc
     4441 gtgttggtga cggcgccctc gtggagcact cggtgggacg cggggccgag gtcggtgacg
     4501 gggcgagcgt cggtccctac tctttcctgc ggaccggtac ggtcctcgat ccaggggcca
     4561 aggtcggggc ttactgcgag gtcaagaact cgcgcgtagg ggcggggagc aagattcctc
     4621 acctctcgta cgtgggggat gcggagatcg gggccgacgc caaccttggg gccgggacca
     4681 taacggcgaa ctacgacggc gagaacaagc accgtacctt ggtgggagac ggggtccata
     4741 caggagtgaa cacgaacctc atcgcgcccg tcacggtcgg cgaggatgct tacacagggg
     4801 ccgggtcggt ggttaacaaa gacgtgccag cgggcaaact ggccgtagga gccccggcgc
     4861 gtgcgatccg ggagtctccc cagcggcgta gaaagagggc gcagcaaggc gattcggcca
     4921 agaaagagac ggagggctag cgcgacgatg atgcagaacg gctatctgac gcagaccaca
     4981 gagaagcggc tgatgatagt cagcggccag ggctatcccg atctatccga acgtattgcc
     5041 gagcggctcg acatagagtt gagcagggtc gagttgaggg aattccccaa tggtgagctg
     5101 tacgccaggt atctggagag cgtcagggga gctgacatgt ttatcgtgca gtcgttgtgc
     5161 gaccctgtca acgagaacct gatgcaactg ctcatcatga tagacgccgc caaaagggcc
     5221 tctgccaaaa ggatcgtggc ggtcatcccg tggtacgcct actcgcgcca ggaccgcaag
     5281 accaagccgc gtgagcccat aaccgcgcgc cttatagcta atatgatcca ggccgcgggg
     5341 gctgagcggg tgatgacgat ggacctgcac tcgggccaga tcgagggcta cttctctttc
     5401 cccgtggatc acctgacggc gatgcacacg ttcgtcgact attttgtgga ccagggtttc
     5461 caggacgcgg aggacgccgt cgtggtcgcc cccgacacgg gcgaggtcaa ggtcgccaag
     5521 cagctcgccg gacacctcag gctcccctgg gccatagtga acaagatgcg cgacaggccg
     5581 ggacactctg aagtgacgca cgtcataggg aacgtagagg gcaagcgggt cgtcatgatc
     5641 gacgacgtag tagccacggg cgggaccctg gtcaacgccg cgaagcgcct tgtggaacaa
     5701 ggcgcgatcg aggttagcgc cgcagccacc cacggcgagt tctcggggga cgcctaccgg
     5761 aagatcgaag actcccccct caaggaggtg gttgtgacca acacgctgcc cttgaaggag
     5821 gatgagccgc ggtctaagat acagacactc aatatcgcac cgatccttgc gagcaccatc
     5881 aagaacgtct tcacggacga gtcggtgagc gaggtcttta tgggcgagaa ccaactcttc
     5941 tagaccgtta ggagcgagac gtggcagaca acgtggatct acaggcgcag gagcgcgaca
     6001 cgcggggcaa gaacgcggcc cgacgcctca gggcctcggg gatgacccct gccgtcctct
     6061 acggcgaggg taacgaggcc ggcgggagca cggcgctagc ggtcccgacg aagaccgtgg
     6121 actacaccct ccatcacttc ggtgacaatg ccctctacaa catagacttt gatggggcta
     6181 cctccacggc ccgcgtcatg gacgcgcagc gtgaccctgt ctccggggtc ctgctccacg
     6241 tcgacttcgc gccggtagac atgctcgagc gcatcgaggt tacggtcccg ctcgctgtcg
     6301 tcggcgaggc cccaggcgtg gaggaaggcg gtgtgctgca gcaggtcgcc tacgagatcc
     6361 aggtggagtc cctccccggc gacatcccgc aggaggtcac gatcgacgtc tcgggcctag
     6421 gtatgcacga gaacctcacc ctgggcgacc tcacgctccc cgagggcgtg acgctgctct
     6481 ctgagcccga agaggtcgcc gttacggtca cggcgccgac ggagatc
//
LOCUS       DASTKM010000098        19096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1256681, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000098 DASTKM010000000
VERSION     DASTKM010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 19096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19096
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256681"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFQ10_03955"
     CDS             <1..660
                     /locus_tag="VFQ10_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9964526.1"
                     /translation="DGYDGPEPVTSSSGPSDWLTERQLERMHARYSAEVTMTDHWLGH
                     FLDKAQALGILDNAMLIVLSDHGHAFGEHGYAGKVAGALYPELTDTIFFIRHPGGKGA
                     DQTSDHYASTHDVAPTILGAMGLQPHPQMGGQDLSVVLDGKEPDPRPHFTAGYHDHVW
                     ARDEKYAMFSLYNGSDPHLFDLENDPKMDKDIASSNPDVVKKMFDEYVIKDAGGPLPH
                     Y"
     gene            703..1320
                     /locus_tag="VFQ10_03960"
     CDS             703..1320
                     /locus_tag="VFQ10_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964527.1"
                     /translation="MRRLLSFLLKWSGILCVPGGLLWALSPTGVSISNLAFHTPNVFW
                     KMFWSAPLLILLGLVGLQLRQSEHSGLLEKLGFFVAVVGLVLIIAGDIGLYWLGIDDI
                     FIVTAPAYKAFRLGLLVLAVGSILFAVTSPREGALPTWGLLPFVLGSMCGLISFSRDF
                     GSFGAVLWALFGVGWAWLGISLVVEGFSSFLRTRRAARDGIAVKH"
     gene            complement(1348..2292)
                     /locus_tag="VFQ10_03965"
     CDS             complement(1348..2292)
                     /locus_tag="VFQ10_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9964528.1"
                     /translation="MKLRTSLLAIGGIGGGLALYNRRLERSGGQLPNRLGGEVRYYRW
                     RGGDLAYSVAGDGEPLLLVHGVYAGASSFEFRKNFEELSKSFRVYALNLLGCGLSERP
                     SRRYEPEDVALQIEDFVREEIGGPAHLVASSLTAALVMPAVVRSGQLFQKLVLICPTG
                     YGILDRPSGRLGDVIYGLFLAPILGNTLYHAIVSERGIRYYLGSMAYHDADLVTEELV
                     EDFYSTAHQPGARYFPAAFVSGKLNLGVADLWMRVPHRTLICWGLEARTVPVREARRF
                     VENNPRCEPRFFKDAALLPHDERARTFNEELKTFLLKK"
     gene            2426..2899
                     /locus_tag="VFQ10_03970"
     CDS             2426..2899
                     /locus_tag="VFQ10_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9964529.1"
                     /translation="MDRERLVDETLMLLPTLMRLVERPSPVEMGEIARRGVATDVQVS
                     PGHIQVLIALARGPRSVGQLAEELGVSPPAATQLVDKLAEHGMVDRHNDPADRRVVLV
                     DYVAGMREVARRIVRDRRRPLHDAMSEMTDGEALAFVKGLRLLAQSFGAAAGEEI"
     gene            2899..6156
                     /locus_tag="VFQ10_03975"
     CDS             2899..6156
                     /locus_tag="VFQ10_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9964530.1"
                     /translation="MQRIVRWCLKNKSVVILATILLIGSGVYATTRLNQELLPDISFP
                     IITISTPVAGAGPDLVDEQVTQPVEDAINGVQGIESVRSTSSQGFSVVVVEFDLDADT
                     EEAESDLQAALDGIQLPSQAGDPEVKSQSASQFPILSLSLAAEDQDLADLTRYAEDEV
                     VSSIEEVDGVASVDLIGGAEKRIQVDLDPDKVKEKGLSIDAVVGALSGAEVDAPVGSV
                     TVNGRETPVSTTSEIGGISALEDLPVGVDGGDTGGAPSTAPADIQGSASPRGAAPPGG
                     APAGAAAAPTRAFAPPAGRAVGTMGSPAAEPVLLGDVAQVRKVGSGLSGISRTNGEPS
                     LGLNVIKETDANTVEVAQEVERVLDDVRDEIGRDQVVVVANAATDVEESVNGLVEEAL
                     VGAVLAILVIFAFLRSVRATLVTAVSLPTSVLAALLFSWVDNLTLNIITLAGLTIAVG
                     RVVDDAIVVLENSYRHVQSGYEPEESALKGTTEVASAITSSTLTTIAVFLPLALVGGI
                     VSKFFVPLSLTVALALVASLIVAVTIIPVLVSIFLRRRKGDRTPQRTDEDASEDGSGG
                     FLPFQAWTQRFGGESDGLLLRLYTPMLRWGLRHRMAVLLLALLTFAGGLGLVRFLPVT
                     FFPPSEERLLIADVELPAGTALGKTSEKLRPFEDFLQQDEGIKSYQVSIGGEDTQDPE
                     SPVRPGNKAQAFINVRESADVRNTLDRVDNEGDELYGENFQIQILSNGPPQGGLEAVL
                     TGGSKEELAKAADIVSDEFRKLEDVNNVESDLSGGNPEVEIKVDPEKAAKAGLSPVQV
                     SGSLGSLLGESTVTTLGETPVVVGVPQGSVDSLDEVLGLPVGSDTTVGAVAEVAEVES
                     PAAVSRTDGERAVTVTGGITSTDTQTVSTEAQRAIADLDLPANVTAHVGGENEDIDES
                     FRNLFLSIIVALVLVFLLLVVFFGSLLVPLVILLAVPLTTVGAFGALYLTNTAISVPS
                     LLGVLLLIGIVVSNAILLVDFAVKAQARYETSDEAILAAGRARLRPILMTAFATIFAL
                     MPLALGLSGGGNGLISSSLAITVVGGLATSTFLTLLVVPVGYSLLKSGRRRKGKA"
     gene            6338..6535
                     /locus_tag="VFQ10_03980"
     CDS             6338..6535
                     /locus_tag="VFQ10_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964531.1"
                     /translation="MKDRFESIPVQVPRSEIEAMGVEIGLDDPVEAARIFDRLKGISW
                     RGDYVRSEEGWVAAWVREVIC"
     gene            6935..8332
                     /locus_tag="VFQ10_03985"
     CDS             6935..8332
                     /locus_tag="VFQ10_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9964532.1"
                     /translation="METPVGTVLAGRYRVESTLGSGGMAVVYRAEDDILGRAVALKTL
                     HHRYAEMPSFRRRFRQEARAMASLDHENIVKVYDISQDGELPFIVVECVSGRDLGDLL
                     GGRSRRRSGRLNEQFVRRMAIQLLHALSYAHRRGIIHRDIKPSNILLTSGGTVKVADF
                     GIARIVEEDDVLMDEAGEIVGSARYMSPEQLRGEDATPRSDVYSAGVLLYHCLTGRPP
                     FSGDVKSLARQHIHKDPQPPRRLNKRITPGMEAVIMKALSKKPRDRYFSANAMLDDIE
                     MKAQPRSAGTTEAPGQARRKARGGALVVASVAVLLLLLGGGGALASGLVGLPQDGSVA
                     GTLSRMNPVETAPPSPPQGAQGESNAEAAADQSGGQNVASVTEAASSQAKRPARPKMV
                     PVPNVTAYYDYYAEDALLRRGFEVRFVYDYQDGFAPRGVTWATDPAAGTLAPRGSTVT
                     VYATPKDLPLPRRLR"
     gene            complement(8480..9004)
                     /locus_tag="VFQ10_03990"
     CDS             complement(8480..9004)
                     /locus_tag="VFQ10_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964533.1"
                     /translation="MSPMQEDPTLTPWRSLGETLEGSFAARARGLLAPDFAILGREGQ
                     EIGRLEIHGAQGAGLGAGDLEARIERSTLSGYRMLTEGAEILAAQPLGAWNAPEIMCL
                     DRLYQGQLSLLRNTARAGPAGERATVHITGGLTNRYYGAVFDAGDAGSLPVAFFLLYL
                     TVALRRKAYVTESS"
     gene            9086..9445
                     /locus_tag="VFQ10_03995"
     CDS             9086..9445
                     /locus_tag="VFQ10_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964534.1"
                     /translation="MELRNKEELRNEILQDWLRSAWVYPFQGPDERTYLRLTPGGRLK
                     MRRRIGELERALGVEGTELVKQEETGTLPAERDRLELAMMVQAYDTERRLMQSQGAIL
                     GTPAVTLEEQADPDTTP"
     gene            9638..11449
                     /locus_tag="VFQ10_04000"
     CDS             9638..11449
                     /locus_tag="VFQ10_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HET9964535.1"
                     /translation="MSLKNRRKRDRRGRNERQSASYGHYSSSLNPFDSGKRSSRRGIA
                     GPIIIICAIVAVLVAADFWLNSGKIHRGVEVGSVSLGGKTPAEARQIVRNRAMGPLKE
                     IDFAGPAHFTRTAREMGVSFNIDQTVDKAYAVGREGNILERLSERLRASFGGRTISPD
                     IDYQPAKARGEVREIASQIDHPPREADVKIYGSEVEVANSRDGYELNLAATMASLDSA
                     IEDMSGKAHLRGDVLDPGVVTAEAEAAAKKARGALSEQLTLKAEGKSWILSPADLGST
                     LDVTKLDGKIDVSLNRDHLDGRLTDVYNDLTVKPVEASYDFDADGDIFVKPGHEGQSV
                     EGEKLLDSIQGGLFEGKREYQVPIIVAKPRYTTDELEANKPSELQGTYRTNYTATTDQ
                     GQTRVENLKIASGAISGTFVAPGDTFSMLEHVANLDYFQTHVIVDGAETVDEGGGLCQ
                     VTSTLYNAALYAGMEITERTAHYSQLPYIRPGMDATVWYGGPGTADDLDMKFKNTSDG
                     YVLLQEYVSNDGYIYANVYGVPDKVKVEMSSEPIFRAEDASKWVTYYERTRNGKVVYR
                     DQWETAYGALIDDEGRKIPTPDVPVAEVDGTYLGPAF"
     gene            complement(11532..12878)
                     /locus_tag="VFQ10_04005"
     CDS             complement(11532..12878)
                     /locus_tag="VFQ10_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964536.1"
                     /translation="MLTQTDKANDLAWRFGGERGGEFAPAGIPVSRLAHEYGTPFYLY
                     HGEMISERVSRVREALGTEVSYSIKANPSLGVCQLVASEREAGAEVASSGELAVALGA
                     GFEPHDIVFAGPGKTDDELRRAVLEGIFAVNVESTNEIDRLARIAREVGKDIGVGLRI
                     NPEAQLMGSGMRMGGTVGQFGMDQAELGEAVQKTLGRAELALRGVHVYTATQVFEVGP
                     LLEHCRNILEISLEAADRAGRPLQMIDFGGGFGVPYFEKMSEFDLDGFADGFRDLLSS
                     YRQDQRLEGCRYLFELGRYLVADAGVYVTKVVDVKETRGKTFVVTDGGMNHHLTATGN
                     MGQVFRKAYPLLNLTRMGGVPEESVAVAGPCCTPLDMFGNNIPLATPEAGDLIGVFYS
                     GAYGYSASNLGFLSHPAPAEVLLWQGGAHLLRPGGRPEDVLSGQRALSKPESSVPG"
     gene            complement(12859..14448)
                     /locus_tag="VFQ10_04010"
     CDS             complement(12859..14448)
                     /locus_tag="VFQ10_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9964537.1"
                     /translation="MRSRPEFTLHDLLYNSVARDPQKTAVVDGDAKHTYEDLERQSGS
                     LGAALSEAGVGRGDRVGIYLDKSWEAVVAMLAASRIGAAYVNVNPLFKAPQVEYLAGD
                     CDIRVMIGDTPKLEELQPKTVQTAFYRGAKPEGQAARSYVDVAEALEGEGLKVDRNVS
                     ESDLGTILYTSGSTGMPKGVSTSQRNVVVGAQIVSTYLENTPEDRILSALPLNFDAGM
                     SQFTTSLRAGATLYLLRSRLPGDLLKALRRHEITGVTGVPPLWALLIRGARSIRENPL
                     THLRYLANTGGRIPQANLDELRDLLEPAGTRIFLMYGLTEAFRSTYLVPEEIHRSSPE
                     QGNCIGKAIPNTEIMVITREGKEAAPGEPGELVHRGPTVAMGYWGNEQATRKAYRPNP
                     LAPPELLDVERVVYSGDTVKRDEEGYLYFLGREDAMIKNQGYRLSPEEVENLLLGSGL
                     VHEACAFGVENPAVGQDVIAAISLRNGVEEGAVDRVREYAINNGPPYMVPKEILVRDE
                     LPKTGSGKIDRKGISSAYSNG"
     gene            complement(14445..14696)
                     /locus_tag="VFQ10_04015"
     CDS             complement(14445..14696)
                     /locus_tag="VFQ10_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET9964538.1"
                     /translation="MVSDNGDARSAIRGWLQENVTGGRDVLADEPLIDNGVMTSLQTV
                     ELVVFLEDTFGIVVEDEEFDEENFGSVEAIARLVESKAA"
     gene            complement(14707..15909)
                     /locus_tag="VFQ10_04020"
     CDS             complement(14707..15909)
                     /locus_tag="VFQ10_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964539.1"
                     /translation="MKAAVEPVESPELEDKIAQLRILSFPDFPEVRDVHYYSALYRWY
                     QDHPLSGKLYRWVSLTEEGEVVGHLAAFPQFYRIDGQRVVAHTPGDYMVLPQHGFQAI
                     MLMRSFFRWTENCVVCDMVPAVIGVETRLGAKVVGDLKYAAKLMNVSRLPMPSVPAPL
                     RRLLNLPQQFAPARGFTDRPGAEAHEVEEYVAPPAPRPRAPIPRPLQELLNGGLRAVD
                     EALGSGLGGDLEVEALDGFDDSFDDLFERVTAVVPCTPEKDAAFLRWRYGPGSPQAPV
                     TVLGVRSRGRMLGYAVLKAAFHGQDGYILDLTTLPGRQDVVRALLRETVRSFRRMGVQ
                     IIRYRFVEAPTSPRSADLRRLGFFYRRGRHHKLLVKFADTSLRETTGDLVNWSLSFGD
                     GEAGFWLR"
     gene            16296..17738
                     /locus_tag="VFQ10_04025"
     CDS             16296..17738
                     /locus_tag="VFQ10_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET9964540.1"
                     /translation="MKEISKTGAPGATRLYNNETERAMKRKRGKRRYARRRKTAGVAL
                     IACFLLVGAVAVYNLTRGDDEIVRGVSIGSVDVGGMSRDEARKAVQEDASATFEEISF
                     GTGSEGFSVSGDDLGIKVDAASAVEEAYSIGRRGNMFQHLSDTLRSYLGGVRVDLDAG
                     YDMDRARSVLEERAEEFNEKPQDASFRVTDSGKVEVEEARNGRVMDQEKTLANLEGAL
                     KNMSGNVAIAEGPPPEPEISTAEVQSYRPKEVIGEYQTDFLWDSNPNRKYNMKLAAKS
                     VNNTVLEPGEVFSFNKLTRSLDYKEAKTFSNGGVGIANGGGLCQVSSTLYMAANYAGL
                     QIVERHPHYAVLPYIKPGFDATVWFGENGWGAMDMQFKNNTDGYIVVREWVDDKGLLN
                     AQILGQPTGKKVEMSTEKIYEDPVKGIKWTTYKKVTEDGEVIRDGFLYTYRYSFNPPP
                     PENAPHYKTTAPRVAGWSDPTNTTGWASPH"
     gene            17745..>19096
                     /locus_tag="VFQ10_04030"
     CDS             17745..>19096
                     /locus_tag="VFQ10_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9964541.1"
                     /translation="MGLFSRRKRWYKRRRSRLPVGVLVVLVLLAGAVYVVTRTLDGTA
                     NFSDPSQKGTPSEPSEEAKSEDAPAKGSDQGQSTPEEPAPDPEQATPEAAAYVALAPE
                     LPGTTPQSIKGVYRSKIDKSWASVRVQPENEKKPFVVFTHRDGQSWKAEKSVRADEPN
                     YARNDVVPLSGVPKDLIEYLYAENVFAAEVPEPKEEKLDLEDLPDAGSADFSSTETVM
                     DDVPESDRERVQKVLEGAKQEIEDYDGVAGLYVWDLDNDSGYGIRPDEQFFAASTIKI
                     PIMVAVYRKVDQGELEFSQQIEIQEEDWAAGAGWLQWEKAGTKQTVGDLLLLMMTQSD
                     NVAANALVRTVGGADHVNEVARSMGAEDTLLYQKVSSERGVVPALDNRSTPRDMATMM
                     QQIAEGKAASEKSCGYMIDLMHENGLDWWLDEGLPPDVDAANKAGWLYRVYDEVGIVE
                     "
BASE COUNT         3658 a         5949 c         6059 g         3430 t
ORIGIN      
        1 gatggatacg atggccccga gcccgtgacc tcgagcagcg ggccctcgga ctggctcacg
       61 gagcgccagc tcgagcggat gcacgcgcgc tactccgccg aggtgacgat gaccgaccac
      121 tggctgggcc acttcctcga caaggcgcaa gcactcggga tcctggacaa cgccatgctg
      181 atagtgctct cggatcacgg acacgctttc ggggagcacg gttacgccgg caaggtcgcc
      241 ggtgcgctct accccgagct caccgatacc atcttcttta tcaggcaccc tggaggcaag
      301 ggcgcggacc agactagcga ccactacgcc tcaacccacg atgtggcgcc aacgatacta
      361 ggtgcgatgg gcctgcaacc tcacccgcag atgggggggc aggacctctc agtcgtgctg
      421 gacggaaagg aaccggaccc caggccacac tttacggccg gctaccacga ccacgtctgg
      481 gcccgcgacg agaagtacgc tatgttcagc ctctacaacg gctcagaccc tcacctcttc
      541 gacctggaaa acgacccgaa gatggacaag gacatcgcct cgtcgaaccc cgacgtggtg
      601 aagaagatgt tcgacgaata cgtgatcaag gacgccggcg gccccttgcc ccactattga
      661 tcgggcgcct gatgctatcg taccggagag gtaagagaag tcttgcgtag gctgttgtca
      721 ttcctcctca aatggagcgg catactgtgc gtgccaggtg gcctgctgtg ggcgctctct
      781 cctactggcg tctccatctc caatctcgct ttccacaccc caaacgtctt ctggaagatg
      841 ttctggtcgg ccccactgct catcctcctc ggcctggtcg gtctgcagtt gcggcagtca
      901 gagcattccg gcctgttgga gaagctgggg ttcttcgtgg ccgttgtggg acttgtcctg
      961 atcatcgcgg gcgacatcgg cctgtattgg ctcggtatcg acgacatctt tatcgtgaca
     1021 gcgcccgctt acaaagcctt ccgactcggg ctgctcgttc tggccgtggg ttctatcctc
     1081 ttcgccgtta catccccccg cgaaggcgct ttgcctacct ggggcctgct gcctttcgtc
     1141 ctcgggtcga tgtgcggcct gatctctttt tcgcgggact ttggctcctt cggcgccgtg
     1201 ttgtgggctc tgttcggtgt cgggtgggcc tggttgggta tctcgctcgt ggtcgagggc
     1261 ttctcctctt tcctgcgaac tagacgtgcc gcccgcgatg gtatcgccgt gaaacactga
     1321 cgcggtgcgg atccaggcca ggcttcccta ctttttcagc aagaacgtct tcagctcttc
     1381 gttgaaggtc ctggcgcgct cgtcgtgtgg caggagggcg gcatccttga agaaacgtgg
     1441 ttcacatcgg gggttgttct ccacgaaacg ccgcgcttcg cggacaggga ccgtacgcgc
     1501 ttcgaggccc cagcagatga gggtcctgtg cggcacccgc atccagaggt cggcgacccc
     1561 caggttgagc ttgcctgaga cgaaggccgc agggaagtat ctcgccccag gctggtgagc
     1621 agtgctgtag aaatcctcga ccagctcttc tgtaacgaga tcggcatcgt ggtaggccat
     1681 gctacccaga tagtagcgga tgccccgctc ggagacgatg gcgtggtaga gcgtattccc
     1741 gaggatcggc gcgagaaaga ggccgtagat cacatccccc agccgtcctg agggacggtc
     1801 caggatcccg tagcccgtcg gacagatcag gactagcttc tggaagagct gcccactccg
     1861 aacgacggct ggcatgacga gcgccgccgt gagagagctg gccacgaggt gggcagggcc
     1921 cccgatctcc tcccttacga agtcctcgat ctgcaacgcg acgtcctcgg gctcgtagcg
     1981 ccgcgaaggc ctctccgaca aaccgcaccc caggaggttc agggcatata ccctgaagct
     2041 cttcgacaac tcttcgaagt tcttgcggaa ctcgaaggaa gacgccccgg cgtagacgcc
     2101 gtgcaccagg agcagcggct caccatcccc cgccaccgag tacgcgagat ccccacccct
     2161 ccaccggtag tagcgcacct caccaccgag ccggttcgga agttgcccac cactcctctc
     2221 caaccgccgg ttgtagagcg ctaatccccc gccaatgccc cctatcgcca ggagcgacgt
     2281 cctcagcttc atcccacctc ctcagacatg ctcgacgcca ggatcgtacc gaaagcgtcg
     2341 gccacattcc tcgttgacaa acctagagcc tgggttagca tttatgcaga ttatataagc
     2401 agcttaaatt tgtaggagca ttcacatgga cagggaacgg ctcgttgacg agaccctgat
     2461 gttactaccg actttgatgc gcttggtcga gcggcccagt cctgtggaga tgggtgagat
     2521 cgcacgccgt ggggtggcta cggacgttca ggtttcgccg gggcacattc aggttttgat
     2581 agcgttggct cgtgggccgc ggtccgttgg acaactcgcc gaggagctag gggtctcacc
     2641 gccggctgcg acgcagctcg ttgacaagct ggccgagcat gggatggtgg accgccacaa
     2701 cgatccggcg gaccggcggg tcgttctcgt agactacgtg gcaggcatgc gcgaggtagc
     2761 caggcgcatt gtgagggacc gtcgtcgccc actgcatgac gccatgagcg agatgacgga
     2821 cggggaggct ctggccttcg tcaagggcct gaggttgttg gcccagagtt tcggcgccgc
     2881 tgcgggggag gagatctgat gcagaggatc gtccgttggt gtctaaagaa caagtccgtc
     2941 gtcatcctgg cgacgatttt gctcatcggg agcggggtct atgcgacgac gcgcctcaac
     3001 caggagctct tgccagacat ctccttcccc atcataacca tctcgacccc cgtggctggc
     3061 gccggaccag atctggtcga cgagcaggta acgcagcctg tcgaggacgc aataaacggg
     3121 gtgcagggga tagagtcggt caggtccact tcctcgcagg ggttctccgt cgtggtcgtc
     3181 gagttcgacc tcgacgccga caccgaggag gccgagtccg acctccaggc ggccctcgac
     3241 ggcatacagc ttccttcaca ggcgggagat cccgaggtaa agagccagtc tgcttcgcag
     3301 ttccctatcc tgtcgctctc gctagccgca gaggaccagg atctcgccga cctcacaaga
     3361 tacgccgagg acgaggtcgt ctcctccatc gaggaagtcg acggcgtggc gagcgtcgac
     3421 ttgatcgggg gggccgagaa acgtatccag gtggatctcg accccgacaa ggtgaaggag
     3481 aagggcctct ctatcgacgc cgtggtcggc gcgctaagcg gggcggaggt ggatgctccc
     3541 gtgggtagcg tcaccgtaaa cggacgcgag acccccgtaa gcaccaccag cgagatcgga
     3601 gggataagtg ccctcgaaga cctccccgtc ggggtggacg gcggcgatac cggcggggcc
     3661 ccttccaccg cgcccgcaga tattcaaggc tctgcctcac ccaggggcgc agctcctcca
     3721 ggtggcgctc cggccggcgc tgccgccgct ccgaccagag ctttcgcccc tcccgccggt
     3781 agagcggtgg gaacgatggg atctcccgct gcggagccgg tgctgctagg ggatgtggcc
     3841 caggtgcgta aggtgggctc gggcctatcg gggatctcgc gtaccaacgg tgaaccgagc
     3901 ctgggcctta acgtgatcaa ggagacagac gccaacaccg tcgaggttgc acaagaggtc
     3961 gagagagtac tcgatgacgt gcgagacgag atcgggcggg atcaggtcgt ggtcgtggcc
     4021 aacgctgcga ccgacgtcga ggagtcggta aacgggctcg tcgaggaggc gctcgtcggg
     4081 gccgtactcg cgatactggt gatcttcgcc ttcctgcgct ccgtacgcgc aaccctggtc
     4141 accgcggtct ctctgcctac ctctgtgctc gccgccctcc tcttctcctg ggtagataac
     4201 ctgacgctga acataatcac cctcgcaggc ctgacgatag ccgtgggcag ggtggtcgac
     4261 gacgcgatcg tggtgctgga gaattcctat cgccacgtgc aaagcggcta cgagccggag
     4321 gaatctgccc tcaaggggac cacagaggtg gccagcgcca tcacctcatc gacgctaacg
     4381 acgatcgccg tctttctccc tctggccctc gtcggcggaa tagtctccaa gttcttcgtc
     4441 ccgctctcgc tgacagtcgc tctcgcgcta gtagcctcgt tgatcgtggc cgtgaccatt
     4501 atccccgtgc tcgtcagcat cttcctcagg cgccgtaagg gagaccgcac gccacagagg
     4561 acggatgaag acgcctccga ggacgggagc ggcggtttcc tccccttcca ggcatggacg
     4621 cagcgtttcg gcggcgagtc ggacgggctg ctcttgcggc tctatacccc gatgttgcgc
     4681 tggggtttgc gacacaggat ggccgtgttg ttgctcgcac tccttacatt cgcaggggga
     4741 ctcgggctcg tgcggttcct gcccgttaca ttcttcccgc cgagcgagga gcgtctcctc
     4801 atcgcggacg tggagttgcc tgcgggcacg gccctcggga agacgagcga gaagctgcgc
     4861 ccgttcgagg acttcctgca acaggacgaa ggcataaaga gttaccaggt ctccatcggg
     4921 ggtgaggaca cccaggaccc cgagtccccc gtcaggcctg gcaacaaagc ccaggccttc
     4981 atcaacgtaa gagagagcgc ggacgtcagg aatacgttgg atcgggtgga taatgagggc
     5041 gacgagctct acggggagaa cttccagatc cagattctct cgaacggccc accccagggc
     5101 gggctcgaag ccgtcctcac cggcggttcc aaggaggagc tcgcaaaggc ggcggacatc
     5161 gtctccgatg agtttcgcaa gctcgaagac gtgaacaacg tggagagcga cctctcgggc
     5221 ggcaaccccg aggtggagat caaagtagat ccggagaagg ccgcgaaagc agggctctcg
     5281 cccgtacagg tctcgggctc gcttggctcc cttctcggcg aaagcaccgt gaccacgctc
     5341 ggggaaacgc ctgtcgtagt tggcgtgccc cagggctccg tggattcgtt ggatgaggtg
     5401 ctggggttgc cggtcgggag cgacactacc gtgggagccg tcgccgaggt ggcagaggta
     5461 gagtctccag cggccgtcag ccgcaccgac ggggagcgtg ccgtcacggt cacgggtggg
     5521 atcacttcta cagacaccca gacggtctcc acggaggccc agagggccat tgccgacctc
     5581 gacctgccgg cgaacgtcac ggcgcatgtc ggcggcgaga acgaggacat agacgagagc
     5641 ttcaggaacc tcttcctctc cataatcgtg gcgctggtgc tcgtcttcct gttactggtc
     5701 gtgtttttcg gctcgctact cgtcccgctg gtgatactgc tcgccgtgcc cctaacgacg
     5761 gtcggggcct tcggagcgct ctacctgacg aatacggcca tctccgtacc ctccctgctc
     5821 ggcgtcctac tgcttatcgg aatcgtggtc tccaacgcca tcctgctcgt cgacttcgcc
     5881 gtcaaagcgc aggcccgtta cgagacctcc gacgaggcta ttctggctgc cggtcgggcg
     5941 cgtctgaggc ccatcttgat gacggccttc gccaccatct tcgccctgat gccgctcgcc
     6001 ctcgggctct ccggcggcgg taacggtctg atctcgtcga gcctcgccat aacggtcgtc
     6061 ggaggcctcg cgacctcgac gttcctcacg ctgctcgtcg tgcctgtcgg ctactcgctc
     6121 ctcaagagcg gccgcaggcg aaagggaaag gcatagaagg gtggagtcaa cacccaccta
     6181 ctgatcggcc gaatttcgac cctgcgtagt gttttgtacg acagtctcgt tcctttggga
     6241 aaggcagact gtccggtatc cgaagaggag gtcggccatc agagagggta gataccggac
     6301 ccccgacagg cagcagcaga tcaggatgcg cgaggtgttg aaggacaggt tcgagagcat
     6361 cccggtccag gtgccgcgta gcgagatcga ggccatgggc gtcgagatcg ggctcgacga
     6421 cccggtcgaa gccgccagga tcttcgaccg gctcaagggt atctcgtgga ggggcgacta
     6481 cgtgaggtcg gaagaaggct gggtcgccgc ctgggtcaga gaagtgatct gctgaagtct
     6541 ttcagcacgt gagcatttca gctcgtcagc ttgtagcgtg tgcgagccct ggctgacaag
     6601 gcggcgcagc cgccgggctg aagtgctgac gagcgaaggc gaaggctgga gcgtgctgac
     6661 tcgctgaagt gctactttgt ttcgagccgc gcacctcgac ttcgacgggc ccctcacacg
     6721 acccagaccg tccggctggc gtagctcgcc aagcaaaacc cccacgatgg ccttcttcgg
     6781 ccggaacgag cctctagatg tgccacgcac gtttgacatg ctccagcatg ttgggtaacg
     6841 tcatagtcgc cctcccgtag tgtacctgga gtgacttcgg gcatatatac ttattgcatt
     6901 ccagggtcca cgtacggtcg aaggtggtaa gaggctggaa acgccggttg gaacggtcct
     6961 cgcaggacgt tacagggtag agagcacgct cggttccggt gggatggcgg tcgtctacag
     7021 ggctgaagac gacatcctcg gtcgtgccgt cgcgctgaag accctacatc atcgctacgc
     7081 cgagatgccc tctttcaggc ggcgcttcag gcaggaggcc cgtgcgatgg cctccttgga
     7141 ccacgagaac atagttaagg tctacgatat ctcccaggat ggcgagctgc ccttcatcgt
     7201 tgtcgaatgt gtctcgggca gggatctcgg ggatctcctc ggtggtcgca gtcgccgccg
     7261 cagcgggcgc ctcaatgagc agttcgtaag gaggatggcg atacagcttc tgcacgcgct
     7321 ctcctatgcc cataggcggg gcatcataca cagggacatc aagccttcca atatcctcct
     7381 cacgtcgggg ggaaccgtca aagtcgctga cttcggcatc gcccgcatcg tcgaggagga
     7441 tgatgtcctg atggacgagg ccggtgagat cgtcggcagc gcccgctaca tgtcccccga
     7501 gcagctcagg ggcgaggacg cgacaccaag gagcgacgtc tactcagcgg gtgtcctcct
     7561 ctaccactgc ttgacgggaa ggccgccgtt ctcgggcgac gtcaagagcc tcgccaggca
     7621 gcatatacac aaagatccgc agccgccgcg cagattgaac aagaggatca cgccaggcat
     7681 ggaggcggtg atcatgaagg ccctctccaa aaagcccagg gacaggtatt tctcggccaa
     7741 cgcgatgctc gacgacatag agatgaaagc gcagcccagg tcggcgggga cgacagaggc
     7801 gcctggacag gcgcgccgca aggctcgtgg tggcgcgctc gtcgttgcct cggtggccgt
     7861 tctgctactc ctcctgggcg gcggaggggc actggcctcg gggctcgtcg gccttccaca
     7921 ggatggcagc gtggcaggca ccttgagccg gatgaaccct gtcgagacgg cacccccatc
     7981 gccgccccag ggtgcgcaag gggagtccaa cgctgaagcc gccgccgatc agtccggcgg
     8041 ccagaacgtc gcttctgtga cggaggcagc gtcctcccag gcgaagaggc cggccaggcc
     8101 gaagatggtc cctgtcccga acgtaacggc ctactacgac tattacgcgg aggatgccct
     8161 cctcagaaga ggcttcgaag tgaggttcgt ctacgattac caggacggtt tcgcaccgcg
     8221 cggggtgacg tgggccacgg acccggccgc ggggacgctg gctccaaggg gctccacggt
     8281 aaccgtctac gcaacgccca aggacctccc gctacccagg cggctccgct aaagagctcg
     8341 aaaggccaag cctttcgaga agcgtgctgg tcagcaagcc gccaagcttc gccagacggc
     8401 ttgtcagcag ttgagcgttt tgccctctat cgcttcgaga ggcgctgtag aaccagtccc
     8461 gctgggcgag cccgcgctct tagctacttt ccgtaacgta ggccttgcgt ctgagtgcga
     8521 cggtaagata gaggaggaag aaggcgacag gtagtgagcc tgcgtcgccg gcgtcgaaga
     8581 cggccccgta gtaccggttg gtgaggcctc cggtgatgtg taccgtagcc ctttccccgg
     8641 ccggtccggc cctggccgtg ttgcgcagga gggaaagctg gccttggtag aggcggtcca
     8701 ggcacattat ctcgggcgca ttccaagctc cgagcggttg tgccgcgaga atctcagcgc
     8761 cttccgtgag catcctgtag ccggagaggg tggagcgttc gatccgcgcc tccagatccc
     8821 cggctcctag tccggctccc tgggctccgt gaatctccag acgtccgatc tcctggccct
     8881 ccctgccgag gattgcgaaa tcaggagcga ggagtcctcg cgcgcgcgcc gcgaaggagc
     8941 cttccagggt ctccccaaga ctcctccagg gcgtgagcgt ggggtcttcc tgcatggggc
     9001 tcatgatacc cttccataag gcgggaaggt ggtggcgttt ccggatggaa gagggtaaat
     9061 tacgcgagaa acggaagaga gctaaatgga gcttaggaac aaagaagagc tcaggaacga
     9121 gatccttcag gactggttgc ggagcgcatg ggtctatccc ttccagggac cagacgagcg
     9181 cacctacctg cgcctgacgc cgggcgggcg gctcaagatg cggcgcagga tcggggagct
     9241 ggagagagcc cttggcgtcg agggcacaga gcttgtcaag caggaggaga cgggcaccct
     9301 tcccgccgag cgcgacaggc tggagctcgc gatgatggtc caggcatacg acaccgagcg
     9361 gcgtttgatg cagagccagg gcgccatcct cggaaccccc gcggtgacgc tggaggaaca
     9421 ggccgacccg gacacgactc cctgatgttc ccctggtggc ggcccactct tgcacacatc
     9481 ggcaaagagg tggcgtactc acccttgccg tgttaggctt tccgggcggc gctcgttcgg
     9541 gccagggcga ggtgtcgcca gccaagagag tgagggggga cctctccaga aatctgtcgt
     9601 tcgggcgcat ggaaggccgc tcgcggaggg tggatatttg agtctcaaga accgaagaaa
     9661 gcgggacagg cgcgggagaa acgaacgtca gagcgcgtct tatggccatt actcgtccag
     9721 cttgaaccct ttcgactcgg gcaagcggag tagccggcgg gggatagcgg gacctataat
     9781 catcatctgc gccatcgttg ccgtgctcgt cgccgctgac ttctggctga actcgggcaa
     9841 gatacaccgc ggtgtggagg tcggcagcgt ctctttgggc ggtaagacac ctgccgaggc
     9901 acgccagatc gtcagaaacc gagccatggg tccgctcaag gaaatcgact tcgccggccc
     9961 cgcgcatttc acccgcacgg cccgcgagat gggtgttagc ttcaacatag atcagaccgt
    10021 ggataaagcc tacgccgtcg ggcgcgaagg gaacatcctc gaacggctct ccgagcgtct
    10081 gcgcgcctca ttcggcggaa ggacgatatc tcctgacatc gactaccagc ctgcgaaagc
    10141 gcggggcgag gtgcgcgaga tcgcctccca gatagaccac cccccgaggg aggccgacgt
    10201 aaagatctac ggctcggagg tggaggtcgc gaactcccgc gacggctacg agttgaacct
    10261 cgcggccacc atggcgagct tggacagcgc catcgaagat atgagcggga aggcccacct
    10321 cagaggcgac gtgctcgacc ctggggtagt gacggctgag gcagaggcgg cggcgaagaa
    10381 ggccagagga gccctctccg agcagctaac gctcaaagct gaggggaaga gttggatcct
    10441 ctcaccggcg gatctggggt cgactctcga cgtcaccaag ctggacggta agatcgatgt
    10501 cagcctcaac cgcgaccatc tagacggccg gctcaccgac gtctacaacg accttacggt
    10561 caagcccgtg gaggccagct acgacttcga cgcggacggc gacattttcg tcaaaccagg
    10621 ccacgaaggg cagagcgtcg agggcgagaa gctactcgac tccatacagg gtggcctctt
    10681 cgaaggcaag cgcgagtacc aggtgcctat tattgtggcc aagccgcgtt acaccacgga
    10741 cgagctcgaa gccaacaagc cgagcgaatt gcagggcacc tacaggacca actacacggc
    10801 gactacagac cagggccaga cgcgggtgga gaacctcaag atcgcttctg gtgccattag
    10861 cggtaccttc gtggcgccgg gtgatacgtt ctcgatgctc gagcacgtcg ccaacctcga
    10921 ctactttcag acgcacgtca tcgtggacgg cgcggagact gtggacgagg gaggcggact
    10981 ctgccaggtc acctcgacgc tctacaatgc ggctctctac gccggcatgg aaataacgga
    11041 gcgcaccgcc cactactcgc agttgcctta catcaggccg ggcatggacg cgacggtctg
    11101 gtacggtggt cctggaacgg cggacgacct ggacatgaag ttcaagaaca cctctgacgg
    11161 atacgtgctc ctgcaggagt atgtctcgaa cgacggctac atctacgcca acgtatacgg
    11221 tgtacctgac aaagtcaagg tggagatgtc gtccgagccc atcttcagag ccgaggatgc
    11281 ctccaagtgg gtaacgtact atgagagaac gagaaacggc aaggtcgtct atcgcgatca
    11341 atgggagacc gcgtatggcg ccctgataga cgacgaaggc aggaagatcc cgacgcccga
    11401 tgtgcccgtg gcagaggtag acggaaccta cctagggccc gcgttttaga cagcccgcta
    11461 cgcccttcgg agctttttgg ctggcgcagc cctgttgccg actcacgttc ggaccccacg
    11521 cggtctggaa attagcctgg tacgctggac tccggcttcg agagcgctct ctggccgctc
    11581 aagacatcct cgggcctgcc gccgggacgc aggaggtgag cccctccctg ccacagtagc
    11641 acttcggcgg gtgcggggtg gctcaggaag cccaggttgg aagcggagta gccgtaagcc
    11701 ccgctgtaga agacgccgat caggtcccct gcctcagggg tggctagggg gatgttgttg
    11761 ccgaacatat cgagcggcgt gcagcagggt cctgccacag caacgctctc ctcgggcacc
    11821 ccgcccatgc gggtaaggtt gagcagcgga tatgccttcc tgaacacctg gcccatgtta
    11881 cccgtcgcag tgagatggtg gttcatgccg ccgtccgtga ctacgaaggt cttgcccctc
    11941 gtctccttca catcgacgac cttggttacg tagacgccgg cgtcggccac caggtagcgc
    12001 cccaactcga agaggtaccg gcacccttcg aggcgctggt cctggcggta ggaggagagt
    12061 aggtcgcgga agccgtcggc gaagccgtca aggtcgaact cactcatctt ctcgaagtag
    12121 gggaccccga agccgccgcc gaagtcgatc atctggagcg gccggccggc ccggtctgcc
    12181 gcctccagag agatctccag aatattgcgg cagtgctcga ggaggggccc cacctcgaag
    12241 acctgtgtcg ccgtgtagac gtggacgccg cgcagggcga gttcggctct accgagcgtc
    12301 ttctgtaccg cctccccgag ctcagcctgg tccatgccga actgtccgac cgtaccgccc
    12361 atgcgcatcc ctgagcccat gagctgtgcc tcggggttga tcctgagtcc cacaccgata
    12421 tctttgccca cctctcgggc tatacgggcg aggcggtcga tctcgttcgt ggactcgacg
    12481 ttgacggcga agatgccctc cagaacggca cgcctgagct cgtcatcggt cttgccagga
    12541 cccgcgaaga cgatgtcgtg gggctcgaaa cccgcgccga gcgccaccgc cagctcccct
    12601 gaggaggcga cttcggcacc ggcctcccgc tccgaggcta cgagctggca tactccgaga
    12661 ctcgggttcg cctttatcga gtaggagact tcggtcccca gagcctccct gacgcggctg
    12721 accctctccg agatcatctc cccgtggtag aggtagaagg gcgtcccgta ctcatgcgcc
    12781 agccttgaga ccgggatgcc ggcaggggcg aactcgcctc cgcgctcccc gccgaagcgc
    12841 caggccaggt cgttggcttt atccgtttga gtaagcactg cttatcccct tgcggtcgat
    12901 cttgccagaa cccgtcttgg gcagttcatc cctgacgagt atctccttgg gcaccatgta
    12961 gggtggcccg ttgtttatgg cgtactccct gaccctgtcc acggcccctt cctccacgcc
    13021 gttccgtagg gagatagcgg cgatcacatc ctgcccgacg gccgggttct cgactccgaa
    13081 ggcgcaggcc tcgtgaacga gcccagagcc gagcaggagg ttctcgacct cctcggggct
    13141 caggcggtag ccctggttct ttatcatggc gtcttcgcgc cccaggaagt acaggtaccc
    13201 ttcctcgtcc cgctttacgg tgtccccaga gtagacgacc ctctcgacgt cgagtagttc
    13261 cggcggggct agcgggttag ggcggtaggc ctttcttgtc gcctgctcgt tgccccagta
    13321 gcccatcgcc acggtggggc cccggtgaac gagctcgcca ggctcgcctg gcgccgcctc
    13381 tttgccttcc ctggttatca ccatgatctc cgtgttcggt atcgccttgc cgatgcagtt
    13441 gccctgctcg ggggaggaac ggtgtatctc ctcaggcacg aggtacgtcg agcggaacgc
    13501 ttccgtcagc ccgtacatga ggaagatcct ggtccccgcg ggctccagaa gatccctcaa
    13561 ctcgtcgagg ttggcctggg gtatccttcc ccccgtgttg gcgaggtagc gcagatgcgt
    13621 caaagggttc tccctgatgg acctggcgcc ccggatcagg agcgcccaca ggggcggcac
    13681 gccggtcacg cccgtgatct cgtgccgcct gagcgctttc aagaggtccc caggcaacct
    13741 ggagcggagc aggtacaggg tcgcgccggc ccgcagggag gtcgtgaact ggctcatccc
    13801 ggcgtcgaag ttgaggggga gcgccgaaag tatccggtct tccggcgtgt tctccaggta
    13861 cgtcgagacg atctgtgccc ctaccaccac gttgcgctgg ctcgtcgaga cgccctttgg
    13921 catccccgtg gatcccgagg tgtagaggat ggtccccagg tcagactcgg agacattcct
    13981 gtccaccttt aggccttcac cctccaaggc ctccgctacg tccacgtagg atctggcggc
    14041 ctgtccttcc ggcttcgcac ccctgtagaa cgcggtctga accgtcttgg gctggagttc
    14101 ctcgagcttc ggggtgtccc cgatcatgac ccttatgtca cagtcgccag cgagatactc
    14161 gacctggggc gccttgaaca gggggttcac gttgacgtag gcggcgccta tgcgcgaggc
    14221 tgcgagcatc gccacgacgg cctcccagga cttgtccagg tagatgccaa ccctgtcgcc
    14281 cctcccaacg cccgcctccg agagtgcggc gccgagggag ccgctctggc gctccaggtc
    14341 ctcgtaggtg tgctttgcat caccgtccac gaccgccgtt ttctgcgggt cgcgcgccac
    14401 ggagttgtaa agcagatcgt gaagtgtgaa ttctggacgt gatctcacgc cgctttgctc
    14461 tccacgagcc tggcgatggc ctcgacggac ccgaagttct cttcgtcgaa ctcctcgtct
    14521 tctacgacga taccgaaagt gtcctcgaga aacacgacga gctcgacggt ctgcaacgac
    14581 gtcatgacgc cgttgtcaat gagcggctcg tcggccagga cgtcgcgccc gccggtcacg
    14641 ttctcctgaa gccatccgcg tatggcggac ctcgcatctc cgttgtcaga aaccatgtca
    14701 gcctccttat ctgagccaga aacctgcttc gccgtcgccg aaagacagcg accagttgac
    14761 gaggtctccc gtcgtctcgc gcaagctggt atccgcgaac ttcaccagca gcttgtggtg
    14821 tctacccctg cggtagaaga agccaagacg ccgcaggtcg gcggacctcg gcgaagtggg
    14881 cgcctccacg aacctgtacc ttattatctg aacgcccatc cgcctgaagg aacgtaccgt
    14941 ctcgcggagc agggcccgca cgacgtcctg acgtcccgga agcgtcgtca gatccagtat
    15001 gtagccgtcc tgaccgtgga aggcggcttt gagcacggcg tagcctagca tcctccctct
    15061 cgatctcacc ccgagcacgg ttaccggtgc ctggggagaa ccgggaccat aacgccagcg
    15121 caaaaacgcc gcatccttct ccggcgtaca gggcaccacc gcagtgaccc tctcgaacag
    15181 gtcgtcgaag gaatcgtcga acccgtcgag cgcctcgact tcgaggtctc cacccaggcc
    15241 gctgccaagt gcctcgtcca cggcccgcag tcccccgttc agcaactcct gcagcgggcg
    15301 cggaatcggc gcgcgcgggc gcggggccgg cggcgccacg tactcctcca cctcgtgcgc
    15361 ctcggcgcca ggcctgtcgg tgaaccctcg ggctggcgcg aactgctgcg gaaggttgag
    15421 gagtctcctc aacggcgccg ggacggaggg catgggtagc cgtgagacgt tcatcagctt
    15481 cgccgcgtac ttcaggtcgc cgaccacctt ggcccctagc cgcgtctcga cgccgatcac
    15541 cgctggcacc atgtcgcaga ccacgcagtt ctcggtccag cggaaaaaag agcgcatcag
    15601 catgattgcc tggaaaccgt gctggggaag caccatgtag tccccgggcg tgtgggcgac
    15661 gacccgctgg ccgtcgatcc tgtagaactg cgggaacgcc gccaagtggc ccacgacttc
    15721 tccctcttcc gtcaaggaga cccagcggta caactttcct gacagaggat gatcctggta
    15781 ccagcggtag agggcggagt aatagtgcac atcgcgcacc tcggggaaat ccgggaacga
    15841 gaggatgcgc aactgcgcga tcttgtcttc cagctccggg ctctcgaccg gctccaccgc
    15901 cgccttcatc ggctgaaccc cgatccggcg ctcctcggaa tggggcgctg ggaagccgat
    15961 ctttccggca gtcctcctcc ctggcttgct atcttacctt ctgatcggtg acgcaaggaa
    16021 ccttccactc tgtagggtct cacggtgcag atccaaaggg caaagtgtaa tggagtggtt
    16081 cgtaaaagcc aagcgacgcg ccttggggtt gagcatctct catgaacagg agctgccctg
    16141 tgtggtagtg ttccaattca ggcaggtgtg atttcgcggc atttactcta gagaggttga
    16201 agcgtcgaac aatttcccgg ataattggcg cctgcgtgtc accgcgaccc cgcagggccg
    16261 gtggcgctaa ttggcatccc aggaggggcg actacttgaa ggagattagc aagacgggtg
    16321 caccgggcgc cacccgactg tacaacaacg agacagaacg ggcgatgaag cgcaagcgcg
    16381 ggaagaggcg ctacgcccgc aggcgcaaga ctgcgggcgt agccctgatc gcgtgtttcc
    16441 tgctggtcgg ggccgtagct gtctacaacc tgaccagggg agacgacgag atagtccgcg
    16501 gtgtcagcat cggctccgtc gacgttggcg ggatgtccag agacgaggcc cgcaaggccg
    16561 tgcaggaaga cgcctcggcg actttcgagg agatctcctt cggcaccggt agtgaggggt
    16621 tctccgtaag tggtgacgac ctcggcataa aggtggacgc ggcctctgcg gtggaagaag
    16681 cctactccat cggacgaagg ggtaacatgt tccagcacct ctccgacacc ttacgctcat
    16741 accttggagg ggtgagggtg gacctcgacg ccgggtacga catggacagg gccaggagcg
    16801 ttctggaaga gcgggctgag gagttcaacg agaagcccca ggacgcctcc ttcagagtca
    16861 ccgatagcgg caaggtagag gtcgaggagg ccaggaacgg gcgcgtcatg gatcaggaga
    16921 agaccctggc gaacctcgaa ggagcactga agaacatgag cgggaacgtg gccatcgcag
    16981 aagggccccc gccagagccg gagatctcca cggccgaggt ccagagttac aggccgaagg
    17041 aggttatcgg agagtaccag accgacttcc tgtgggactc gaacccgaac cgcaagtaca
    17101 acatgaagct ggccgccaaa tccgtgaaca acaccgtact ggaaccgggc gaggtgttct
    17161 cgttcaacaa gctgaccagg tcactcgact acaaggaggc gaagaccttc tccaacggcg
    17221 gcgtcggcat cgccaacggt ggtgggctct gccaggtgtc ctcgacgctg tacatggcgg
    17281 ccaactacgc ggggctccag atcgtcgagc gtcacccgca ctacgccgtg ctcccctata
    17341 taaagcctgg ttttgacgcg acggtatggt ttggggagaa cggctggggt gccatggata
    17401 tgcaattcaa gaacaatacc gacggctaca tagtagtccg cgagtgggtc gacgacaaag
    17461 gcttactcaa tgcccagatc ctcggccagc ccacgggcaa gaaggtcgag atgagcacgg
    17521 agaagatcta cgaggacccc gtcaagggga tcaagtggac tacctacaag aaagtgaccg
    17581 aagatggcga ggtgatccgc gacgggttcc tctacacgta ccgctatagc ttcaacccgc
    17641 cccccccgga gaacgcgccg cactacaaga ctaccgcccc cagggtagcc gggtggtccg
    17701 accccacgaa caccaccggt tgggcaagcc cccactagcg cgccttgggt ctgttctctc
    17761 gtcgtaagcg gtggtacaag cgtcgccgta gccggctgcc ggtcggcgtt ctggtcgtgc
    17821 tggtgctcct ggccggggct gtgtacgtcg taaccaggac cctggatggg acggccaact
    17881 tctccgaccc ttcacagaag ggcacccctt ccgagcccag cgaggaggcg aagtctgaag
    17941 atgcccccgc gaaggggtcg gatcaggggc agtccacgcc ggaggagccc gcgcctgatc
    18001 ccgagcaggc cacgcctgag gccgcggcgt acgtcgccct ggcccccgag ctgccaggga
    18061 cgacccccca gagcataaag ggcgtgtacc ggagcaagat agacaagtcg tgggcctccg
    18121 tacgcgtcca gcctgagaac gaaaagaagc ctttcgtcgt ctttacccac agggacggcc
    18181 agtcgtggaa ggccgaaaag tccgttcgcg ccgacgagcc gaactacgcg aggaacgacg
    18241 tggttccgct ctccggcgta ccgaaagatc tcatcgagta cctctacgcc gagaacgtgt
    18301 tcgcagccga agtccccgag ccgaaagagg agaaactgga cctcgaagac ctgcctgacg
    18361 ccggatcggc ggatttctcg tctacggaaa cggtgatgga tgatgtgccc gagtccgacc
    18421 gcgagcgcgt gcagaaggtc ctcgaagggg cgaagcagga gatcgaggac tacgacggtg
    18481 tggcaggtct ctacgtgtgg gacctcgaca acgactcagg ctatgggata agacccgacg
    18541 agcagttctt cgcggcctct accataaaga ttcccatcat ggtggccgtg taccgcaagg
    18601 tggaccaggg tgagctcgag ttctcgcagc agatcgagat ccaggaagaa gactgggccg
    18661 ccggagcagg atggctacag tgggagaagg cagggaccaa gcagaccgta ggcgacctgc
    18721 tcttgctgat gatgacccag tcagacaacg tcgccgccaa cgccctggtg cgcaccgttg
    18781 ggggagccga ccacgtcaac gaggtcgccc gttctatggg ggcagaggac acgctgctct
    18841 accagaaggt gagtagtgag aggggcgtcg ttccggccct cgacaacagg agcacgcccc
    18901 gcgacatggc taccatgatg caacagatag ccgaaggcaa ggccgcgagc gagaagagtt
    18961 gtgggtacat gatcgacctc atgcacgaga acgggctcga ctggtggctc gacgagggcc
    19021 ttcccccaga cgtggatgcg gcgaacaagg ccggctggct ctacagggtc tacgacgagg
    19081 tggggatagt cgagca
//
LOCUS       DASTKM010000099         5131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1080221, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000099 DASTKM010000000
VERSION     DASTKM010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080221"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1220
                     /locus_tag="VFQ10_04035"
     CDS             165..1220
                     /locus_tag="VFQ10_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964542.1"
                     /translation="MFESDLEDNVFRRRLMKEVAPGVPVHFSFYSPSVKVQVMHPAEN
                     LPGANGRYIGDAVTIEFVPDDAAAVERVREYIWHWEGYNAFTAVRKINHLRRGVCPES
                     VQGILERVRGIDKRAAAKTAEVLDPEELWELLSRGDFKTLARLPGMGEKRSRAAVEFY
                     ENERSDRRFLYAANRNDRFRGKPVITELDLGGDLEAQVWEHAVLARRLGDPDPLGPEE
                     PPGHTTVRNANLVHPLPMTPSLMSRGVYHSEQVGLFADTLRRVLLDGERLVGVGALRI
                     QRGKLFHTAKMAGVGMKTRARVLASGLLDDEYTYESLVAIPGVTEAQARTIARAAMAV
                     KEGPVPIGAARTEVRVS"
     gene            1210..3009
                     /locus_tag="VFQ10_04040"
     CDS             1210..3009
                     /locus_tag="VFQ10_04040"
                     /inference="COORDINATES: protein motif:HMM:NF005906.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HET9964543.1"
                     /translation="MSPSIYGFVKEHEPVSPEEISTRFLSLASPNGDARALVQRLVEG
                     DSRFIWEGEDLRTAQAGSLALGEAPYVIFDVETTGSAAGKGGAITEFGALKLVRGEVV
                     DQFSTLVNPGRPIDPFVVRLTGITDRMVSDAPFIWEVMPRFEEFVEGCVLVGHNVHFD
                     CSFVTAARGGSPLPNEVLDTLKLARCLVPGLKRYRLSALVSHFGVRQAPNHRALSDAA
                     ATTGVFRRLLKLLGSAGVKSVGEALTIRGGRGRIKPQKQHLASGVPNTPGVYYFVDKH
                     GTTLYVGKAKDLKARVRTYFNGGDGRRKVGRLVEEVAEVRVRETESELHALILEAREI
                     KRLLPRYNSVGRDDKASWFIRLDLGEPYPVPERVSTNSSEGGLIHLGPYRSAGVLDTC
                     IEALGRIFPLKRCSGDGEVCFYGQMGRCAPCMGMDEQEYRERVIDEVVALLRGEGGEE
                     HLRALVRERERLAAGLEFEAAARLRDLISGIERIRLSRTVVDAEGVQAVVAPSTEPGV
                     VEVFILCAGGLIAHRGFEPGDATGLGRFAEEVLARPQENGSRPGEEGSNEARIVAAYL
                     RRRQAVVEAVRLSEARDLLKAAQRVAEAAAPLT"
     gene            3128..3805
                     /locus_tag="VFQ10_04045"
     CDS             3128..3805
                     /locus_tag="VFQ10_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9964544.1"
                     /translation="MTRKILLVDDEPSLQKMLTHALEREGFQVQVAGDGEAALEAFKT
                     FEPHLIVLDIMLPKLDGTEVIRRIRAQSDVPVIMLTARDDEIDRVVGLELGADDYVTK
                     PFAVRELVARVRAIMRRAAVPAGQRPDELTYDRLRINLPSRRVSADGEEVDLTYTEFE
                     LLVTLASSPGRVFSRSALLRRVWGDEFRDERTVDVHIRHLREKIERDPRNPEFIHTAR
                     GVGYVFR"
     gene            3792..>5131
                     /locus_tag="VFQ10_04050"
     CDS             3792..>5131
                     /locus_tag="VFQ10_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9964545.1"
                     /translation="MFSAKERSSPTLDFRRKASKLLASKKGGEFKPRLSMRIWLTALF
                     VLVTAFAAITAYEIVHPILYETVNRSSEAAFKQVGDQFEDQVRRLRERGEPLTEQRIN
                     AFARSHGLQWGIVRAEGGQQTQGDLENWSAGPVRRAVEDPRRPSDDMEQIETGVHKGQ
                     LQATYAHPINVDVKGKAEPQPRALVFVKFFTQSDVENVRAAMRNIERLALLAGALALL
                     IAGFAGYFAAVLISRRVSRLGLAAEHLAAGNFDARITTRVEDEVGSLGATFNAMAVSL
                     KGAFDQIEQEKERGSAILDGMTDAVVGVDRALNPVFLNPRARKLLESSDHAFHNRLQE
                     VLAKTRYSGPVTDSEAKAGDRIIEIRAAPLEDGALAILRDVTEERHMQRVKAEFIANA
                     SHELKTPLTALSGYLEMLEDEEDERVRAEFLNDMRAQTGRLQSLARTLLDLSRL"
BASE COUNT          982 a         1555 c         1736 g          858 t
ORIGIN      
        1 gccggagagc ccgtcgaggc catagtgttg cggcaggaaa agctcgtcgg ctagtcagcc
       61 tgttagcacg gcgtaagccg gagcgtgctg actggctgac aaggtgaaat gctgacgagt
      121 tgcctgttcc aggcgacgtg ctgaaatgct aaaatgaggg accgatgttc gagtcagatc
      181 tcgaagataa cgtcttccgc aggcggctga tgaaggaggt tgcaccgggg gttccggtgc
      241 acttctcgtt ttattcgcct tcggtcaagg tacaggtgat gcaccccgcg gagaatctcc
      301 ctggggccaa cggccgctac atcggggacg ctgtcaccat cgagttcgtc cccgatgacg
      361 ctgcggccgt cgagcgggtc cgcgaataca tctggcactg ggagggctac aacgccttca
      421 cggcggtgcg caagatcaat cacctcagac gcggcgtctg ccccgagagc gttcagggca
      481 tcctggagag ggtccgcggc atagacaagc gggcggccgc gaagacggcc gaggtcttgg
      541 atcctgagga gttgtgggag ctactctcca ggggtgactt caagacgctg gcgcggctac
      601 caggtatggg cgagaaacgc tcccgtgccg ccgtcgagtt ctacgagaac gagaggagcg
      661 accggcgttt tttgtatgcc gccaaccgca acgaccgttt ccggggcaag ccggtgatca
      721 cggagctaga cctggggggc gacctcgagg cgcaggtgtg ggaacacgcc gtgctggcgc
      781 gaaggctggg cgaccccgac ccactcggac ccgaggagcc tcccggtcac actaccgttc
      841 gcaacgcgaa cctcgtgcat ccgctcccga tgacgccttc actgatgagc cggggcgtct
      901 accactctga gcaggtcggg ctcttcgccg atactttgag acgtgtcctt ctagacgggg
      961 agaggttggt cggtgtcggc gccctacgca tccagcgcgg caagctcttt cacaccgcca
     1021 agatggccgg cgtcgggatg aagacgcggg ccagggttct tgcgagcggg ctgctcgacg
     1081 acgagtacac ctacgagagc ctcgtcgcca tccccggcgt cacggaggca caggccagga
     1141 ccatcgccag ggccgcaatg gctgtgaaag aggggcctgt ccccataggg gcggcgcgga
     1201 cggaggttcg tgtctcctag tatctacggt ttcgtgaaag aacacgagcc tgtgagcccg
     1261 gaagagatct ccacaagatt cctctcgctc gcgagcccga acggtgatgc ccgggctctc
     1321 gtccagaggc tcgtcgaagg agattccagg ttcatctggg agggcgagga cttgagaacc
     1381 gcgcaagccg gctcgctcgc gctcggggag gctccttacg tcatcttcga cgtggagacc
     1441 accggttctg cggccgggaa ggggggcgcc atcacggaat tcggagccct caagctcgtg
     1501 cgcggggagg tagtagacca gttctcgacg ctcgtcaacc cgggacgccc gatagacccc
     1561 ttcgtggtac gcctgaccgg cataacggac cgcatggtct cggatgcccc ttttatctgg
     1621 gaggtcatgc cacgcttcga ggagtttgtc gaggggtgtg ttcttgtcgg gcacaacgtc
     1681 catttcgact gctccttcgt cacggcagca agaggtggga gcccactccc caacgaggtc
     1741 ctcgataccc tgaaactcgc ccgatgcctg gtgccaggtc tcaagcgcta ccgactgagc
     1801 gcgctcgtca gccacttcgg tgtgcgccag gcgccgaacc acagggccct ctccgacgct
     1861 gctgcgacga cgggggtgtt ccgtagactg ctgaagctcc tgggctccgc aggcgtaaag
     1921 agcgtcggag aagcgctcac tatccgcggg ggaaggggtc gcatcaaacc ccagaagcag
     1981 cacctggcga gcggggtgcc caacacgccg ggcgtctact acttcgtcga caagcacggc
     2041 accaccctct acgtcggcaa ggcgaaggac ctgaaagccc gcgtcaggac ctacttcaat
     2101 ggtggggacg gacgccgcaa ggtcggacgt ctcgtcgagg aggtcgccga ggtacgcgtc
     2161 agggagacag agagcgagct tcacgccctc atcctcgagg ccagggagat aaagcgcctg
     2221 ttgccgcgtt acaactccgt cggtagggac gacaaggcga gctggttcat aaggctcgac
     2281 ctgggtgaac cctaccctgt gcccgagcgg gtctcaacga attcgtctga gggcggcctc
     2341 atccacctcg gcccttacag gagcgccggg gtgttggaca cctgcatcga ggccctcggc
     2401 aggatcttcc cactcaagag gtgctctggt gatggggagg tctgcttcta cggccagatg
     2461 ggacgctgcg cgccttgcat gggcatggat gagcaggaat accgcgagcg ggtcatagac
     2521 gaggtggtcg cgcttttgcg cggcgagggt ggcgaggagc acctcagggc gctcgtgagg
     2581 gagagggaac ggctggccgc cgggctggag ttcgaggctg cggccaggct gcgcgacctg
     2641 atctccggta tagagcggat acgcctctcc aggacggtgg tggatgctga gggggtgcag
     2701 gccgttgtcg ccccatcgac ggagcctggt gtggtcgagg tcttcatcct gtgcgcaggc
     2761 ggacttatag cacaccgcgg cttcgagccg ggcgatgcga ccggactggg gcgcttcgcc
     2821 gaggaggtgc tggcgagacc ccaggagaac ggttcccgtc ccggggagga aggctcgaac
     2881 gaggcgcgaa tcgtcgctgc ctatctaagg cgtcggcagg cggtagtcga ggccgtgcgt
     2941 ctaagtgaag cgcgggatct tctgaaggcg gcccaacggg tcgctgaggc cgcggccccg
     3001 ctgacgtgag gacccgccgg gtcgggaggc tttgtactcc atcatgctaa aatagaacca
     3061 aggtgaattg tcggttcctg cgtacaagaa gagagtttag ccaggtatgg tacaggatct
     3121 acagggtgtg acgcgcaaga tacttctggt cgacgacgag ccgtcgctcc agaagatgct
     3181 cacgcatgcc ctcgagcgcg aggggttcca ggtccaggtc gctggtgacg gcgaggccgc
     3241 tttggaagcg ttcaagacgt tcgagccgca cctgattgtg ctcgacatca tgctccccaa
     3301 gctcgacggc acggaggtca taaggaggat cagggcgcag agcgacgtgc ccgtcatcat
     3361 gctgaccgcc cgcgatgacg agatagacag ggtcgtgggg ctcgagctcg gggcggacga
     3421 ctacgtcacc aagcctttcg ccgtcaggga gctggtggcg cgcgtcaggg cgatcatgcg
     3481 cagggccgcg gtaccggcgg gccagcgtcc cgacgaactg acctacgaca ggctcaggat
     3541 caacctgcca agtcgcaggg tgtcggccga cggtgaagag gtggacctca cctacaccga
     3601 gttcgaactc ctggtgaccc tggcctccag cccaggcagg gtgttctcgc gcagcgcgct
     3661 tctgcgcagg gtgtggggcg acgagttccg cgacgaacgc acggtggacg tccatatccg
     3721 ccacctccgc gagaagatag agcgcgatcc caggaatccc gaattcatcc acaccgcgcg
     3781 gggtgtcgga tatgttttcc gctaaggaac ggtcgagccc gactctagac ttcaggcgaa
     3841 aggccagcaa gctgctcgcc tcgaagaagg gaggcgagtt caagccccgc ctgagcatgc
     3901 gcatctggct cacggcgctc ttcgtgctgg taaccgcctt cgcagcgata accgcctacg
     3961 agatcgtgca tcccatcctc tatgagaccg tcaaccggtc cagcgaggcc gccttcaagc
     4021 aggtcgggga ccagttcgag gaccaggtgc gacggttgag ggaacgcggc gaacccctta
     4081 ccgagcagcg catcaacgcg ttcgcccgct cccacgggtt gcagtggggc atagtcaggg
     4141 cagagggtgg ccaacagacg caaggggacc tggagaactg gtccgccggg ccggtaaggc
     4201 gtgccgtgga ggatcccagg aggccgagcg atgatatgga acagatcgag acgggcgtcc
     4261 acaaaggaca gctgcaggcc acttacgccc atcccataaa cgtggacgtc aaaggcaagg
     4321 ccgagcctca gcctcgggcc ctggtcttcg tcaagttctt cacacagagc gacgtcgaaa
     4381 acgtgcgggc ggcgatgagg aatatagagc gattggcgct actcgccggc gccctggcgc
     4441 tgctcatcgc cgggttcgcg gggtacttcg cagctgtctt gatctcccgg cgcgtcagcc
     4501 gtctggggct tgccgccgag cacctggcag ccggcaactt cgacgcgagg ataacgacgc
     4561 gcgtcgagga tgaggtcggc tctctgggcg cgacgttcaa cgctatggca gtctccctca
     4621 agggggcttt cgaccagata gagcaggaga aggagcgcgg gagcgccata ctcgacggca
     4681 tgacggacgc ggtcgtcgga gtggacagag ccctgaaccc ggtctttttg aacccccgcg
     4741 caaggaagct cctcgagtcc tccgaccatg ccttccacaa ccgcctgcag gaggtactcg
     4801 ccaagacccg ctacagcggt cccgtgacgg attccgaggc gaaggcaggg gacaggatca
     4861 tcgagattcg ggccgccccc ctcgaggacg gggcgctcgc catcctgcgc gacgtcacgg
     4921 aggagcgcca catgcagcgc gtcaaggcag agttcatcgc caacgcttcc cacgagctca
     4981 agacgcctct gacagccctc tccggctacc tggagatgct cgaggacgag gaggatgaac
     5041 gggtcagggc ggagttcctg aacgacatgc gagcccagac cgggcgcctg cagagcctgg
     5101 cacgcaccct gctcgacctc tcgcgcctcg a
//
LOCUS       DASTKM010000100        10920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_551622, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000100 DASTKM010000000
VERSION     DASTKM010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10920
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551622"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..1898
                     /gene="crtI"
                     /locus_tag="VFQ10_04055"
     CDS             381..1898
                     /gene="crtI"
                     /locus_tag="VFQ10_04055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HET9964546.1"
                     /translation="MGALASSSTRPNKRVAVVGAGMGGLAAAIRLAAKGFEVEVFEKN
                     AQVGGRVGRVETEGFAFDTGPSLLLMTDTYRELFRSAGRDLDDYLRLIPLDSNYRVTF
                     GDGDTLTIRRTLPEMIDELERIEPGVTPRFYRFLEDACNKYRLGRSEFVERDFDSARD
                     FFGLRNLRLLLKTRALNNYYRQVSTFFRTEKLRQAFSLQTMYLGLSPFEAPAVYSLLP
                     YTELAEDGLWFPEGGMYSLVEAMERLVTELGVRLHLNSQVERVVVTKGRARGVVVEGS
                     EREADAVLANADLPYAYRELLAGSADKDFRLKRREKLEYTASAFMLYLGVDGKLENMV
                     HHNFYLSGRYRENFEAIFRDRTLPQDPSFYTVVPSKTEPRMAPEGMESLFVLVPVPHL
                     GPNVDWDRDGDAFREKVYGLLERRCGLDRSRVVYEKARTPLDWRDEYNLEEGAAFGIG
                     HGIFQVGYFRPPMVSKAVQGMYFVGASTRPGTGVPLVTIGARLVADRIGREIGLS"
     gene            1895..2848
                     /locus_tag="VFQ10_04060"
     CDS             1895..2848
                     /locus_tag="VFQ10_04060"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HET9964547.1"
                     /translation="MKLDPAGGKKLDGSLSLAESYELCRKIQKAHSRTYYFSTRLFPA
                     EVRPRVHALYAFMRYADEIVDTPHDLPLDAQLSVLEEFEAETMAAVSGEQVPNPVLRA
                     YADTVLACGIEPETITAFMESMKMDTRVFRYQTFSDLEAYTYGSAAVVGLMMCRVVGV
                     AEGKADTHAEALGIAMQLSNFLRDLGEDWRRGRVYLPLEDLAHFGYTEQDLALGVVDE
                     RFVALMRFEIDRARRLYEVADGGIGYIPRGRRFPVVVARKLYAAILDRIEAQRYDVFS
                     RRAQVSRPAKLLVAAGCAASDPGEIVDRIRYRRTRHTDAPD"
     regulatory      2907..3019
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3084..4937
                     /locus_tag="VFQ10_04065"
     CDS             3084..4937
                     /locus_tag="VFQ10_04065"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HET9964548.1"
                     /translation="MSPNLRELLEARVLIGDGAVGTLLGERGVGFGHPYARANLSHPE
                     MVTDIHVEYLRAGADAIETNTFAANRFKLETHDLQERVREVNAEGARLARKAARTVAG
                     TGERALVLGAIGPLGRPLVPVGPVSPDRAKYVFLEQAEALLEGGADAILLETFTDLSE
                     LRLAYEAVETLGAPVLAYKTFVEDGETLAEGLPERAAREISSWGADLTGANCTVGPQR
                     MVEIIGQMAVGAGPVAAFPNPGLPQLVSGHIRFQSDVAHFAEHGVKLAEAGARLVGGC
                     CGTTPAHVRALSGALRDFRVEGKGARRRVTVVEQAGRTEVAAEPVSEFAERLRTGFAV
                     TVELDLPRGNAIAQVVEAARRLKERGVHAIDISDGARARLRMHPVAAARIVQEETGIE
                     VVAHISCRDRNIIGLQADLLGAAALGVKNILAVTGDPAQIGDYPEATSVFDTDSVGLV
                     HILSRMNRREDLAGNHIGEPPGFLVGASFNPTSEDLAQEVEKLRRKVEAGAHAFWTQP
                     VFELRALENALEHIGDNGVCILLGLMPLRSAKQAEFLHHEVPGIHIPKHVRENLANLP
                     PEDAPKYGVEVAQKLLVEARPLIGGAYIMPPASAPDLAGDVLQAISSPVGR"
     gene            4944..8675
                     /gene="metH"
                     /locus_tag="VFQ10_04070"
     CDS             4944..8675
                     /gene="metH"
                     /locus_tag="VFQ10_04070"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET9964549.1"
                     /translation="MVLQSTKNEERQARLYEFRALLEQRIVLLDCAMGTMIQSYGLSE
                     GDYRGKRFADHPGEQKGNNDLLSITQPGIISDIHEAAMDAGSDIIETNTFNSTSIAQT
                     EYRLEDLAYDLNYEAACVAREAADAYTKKTLQKLRFVAGALGPTNRTASLSPDVNNPG
                     FRNITFDELAKSYAEAARGLVEGGVDLLLIETVFDTLNARAAIFGITSYLEEQGLDVP
                     IMISGTITDASGRTLSGQVTEAFWNSVRHANPISVGLNCALGSKELRQYIGELSRISE
                     VPISAYPNAGLPNEFGEYEETPEMMAAEIREWAEAGWLNIVGGCCGTTPDHLRAIAEA
                     VSGHPPREIPEIPKKLRLSGLEPCNIGPDSLFVNVGERTNVTGSARFKDLISSEDYET
                     ALDVARQQVENGAQIIDINMDEGMLEGEESMVRFLDLVASEPDICRVPVMIDSSRWSI
                     IEAGLKRVQGKAVVNSISLKEGEEEFVRQARLLKRYGAAVIVMAFDEKGQAETKERKV
                     EICRRAYGVLTEGVGFPPEDIILDPNVFAIATGIEEHDNYGVDFIEAVREISSTLPHA
                     KTSGGISNVSFSFRGNNPMREAIHAVFLYHAIKAGLTMGIVNAGQLAVYDEIEEDLRE
                     AVEDVVLNRRPDSTEGLLALAQRYRDRGDSVTDEQEREWRGWPVEKRLEHALVKGIAE
                     FVEEDAEEARLQAGRPLDVIEGPLMHGMNVVGDLFGSGRMFLPQVVKSARVMKKAVAY
                     LIPFMEENGDEARKPNGTVVMATVKGDVHDIGKNIVGVVLQCNNFEVIDLGVMVPSAK
                     ILQTASEKGADAIGLSGLITPSLDEMVHNAKEMQREGFDIPLLIGGATTSQMHTAVKI
                     APGYEQPVIHVKDASRAVGVVQNLVSEGRKVDYAEQIAADYERVRRKHEGRRSKTTLA
                     TLESARANRTKIDWEGYSPPKPNVLGVQTFDDYDLREIKPYIDWTPFFHSWQLKGSHP
                     RILDDPEKGEEARTLFADAQELLDRIITEKWLTARAVFGLFPANALEDDTLEVYTDES
                     REEVLASLDFLRQQKQKPPGRPNRSLADFVAPRSMGLEDYVGLFAVTAGLGADEAARR
                     FEEDNDDYNAIMVKALADRLAEAFAELMHRRVRTEFWGYAADEDLDNEALIREEYGGI
                     RPAPGYPACPEHTEKRTLWRLLDVGNNAGITLTESCAMSPGAAVSGYYFSHPDCRYFG
                     VAEIGRDQVEDYAERKGWTLEEAEKWLSPNLGYDVEHKA"
     gene            complement(8757..9428)
                     /locus_tag="VFQ10_04075"
     CDS             complement(8757..9428)
                     /locus_tag="VFQ10_04075"
                     /inference="COORDINATES: protein motif:HMM:NF021998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HET9964550.1"
                     /translation="MTQQDHATQDQIRPETSTGHYLLEVLGILCFVLLLLLIGVDVYQ
                     GMIDFGDLWLAPILAVLAYLAADFLSGFVHFLADNFGSYDTPIIGPNFIEPFREHHID
                     PKGIVGNDFVDANGNNSLASLPFMLGVWIIVPLETTYYGYLFGTFSLFLCLAAFLTNQ
                     FHKWAHMDIPPAFVGWLQAWGDPLQGAPRHPSREPLRHLLLHHRGVLETLYWIAPASS
                     SGWSV"
     gene            complement(9577..10206)
                     /locus_tag="VFQ10_04080"
     CDS             complement(9577..10206)
                     /locus_tag="VFQ10_04080"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HET9964551.1"
                     /translation="MGNKGGGLGTTLVLALVIVVLSLALGVGLARYGLLLPIVGPLLG
                     EKPPRTTTGPVVVEGIRELDQLATVRWTESVPVTRETGGDILDRLFDGERVLVIATGE
                     VEAGVDLGDIGKEDVSVNSDTVTIDLPEPQILSASLDEEKTRVYDRDFSPLNVRPDDD
                     LVEEARLQAVKKIRDAARENGILVTAEHNAEDSVRAFVTTLGFEEVRFR"
     gene            complement(10315..10527)
                     /locus_tag="VFQ10_04085"
     CDS             complement(10315..10527)
                     /locus_tag="VFQ10_04085"
                     /inference="COORDINATES: protein motif:HMM:NF025533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HET9964552.1"
                     /translation="MAGPEDILSRYEPYAPTFRPGEKAPISGTYICDRGTMLPPVRVQ
                     LSKGEEFPEAGGLGGHTRWRETNIQA"
     gene            complement(10527..>10920)
                     /locus_tag="VFQ10_04090"
     CDS             complement(10527..>10920)
                     /locus_tag="VFQ10_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET9964553.1"
                     /translation="RGLKVETSRTSESADYTNLVELRMGENAVSGTLFGPRMQPRLVE
                     AMGFDFDVVPEKYMLFIRNEDRPGMIGKLGSILGEHGINIGNMAVGRGEPGSRAAMAV
                     TVDEPVPEEVLNALVETPGFNEARSVTL"
BASE COUNT         2164 a         3401 c         3496 g         1859 t
ORIGIN      
        1 ggtccaggcc cacagcctct ggcgtcactg ggacgagccg cgcatcggct ggtatcaggg
       61 agcccaccag cgctttatac gcgcctccga aggtcgtgag gtcctcgaag agaccctccg
      121 cgccgccgac atgcttccca aagagacgtc aggcactgcc tctcatagcg cttagcggaa
      181 agggcacccc ttccgctaag cgcgctggtc agccagtaag ccagtgctcg cgccagcgaa
      241 agctgaagtg ctgacaagcc gcccgcaggg cggcgagctg acttgctgaa gtgcccttgt
      301 aagctccttt actcggaggc gcggtgaacg ctgttagcat ctgcaacgta tactctcgac
      361 gtactctcgg aaaaaggaag gtgggcgctc tggcgtcgtc atcgaccagg ccgaacaagc
      421 gggtggcagt cgtcggggcc gggatgggcg gcctggcggc ggcgatccgg ctggccgcca
      481 aggggttcga ggtcgaagtc ttcgagaaga acgcccaggt cggcggacgc gtgggacgag
      541 tcgagaccga aggcttcgcc ttcgatacgg gcccatcact cctcctgatg accgatacct
      601 atcgcgagct cttccgctct gccgggcgcg acctggacga ctacctcagg ctcataccgc
      661 tggactccaa ctaccgcgta actttcggtg acggcgacac cctgaccatt cgccgtactc
      721 tccccgagat gatagacgag ctggaacgca tagagcctgg ggtcacaccg cggttctacc
      781 gcttcctcga agacgcctgc aacaagtacc gcctgggccg cagcgagttc gtggagcgcg
      841 acttcgacag cgccagggac ttcttcggac tgcgcaacct caggctcctg ctgaagacca
      901 gggcactcaa caattactac cgccaggtct ccacgttctt ccgaaccgag aagctcaggc
      961 aggctttctc tttgcagacg atgtacctcg gcctctcccc tttcgaggct ccggcggtct
     1021 actcactcct cccctacacc gagctcgccg aggacggcct ctggttcccc gaaggcggga
     1081 tgtattccct cgtcgaggcg atggagaggc ttgtgaccga gcttggcgta aggctccacc
     1141 tgaacagcca ggtcgagcgt gtagtcgtca ccaagggcag ggcgcgcggc gtcgtggtcg
     1201 aaggaagcga gagagaggcc gatgccgtgc tcgccaacgc agacctgcct tacgcctacc
     1261 gcgaactcct cgccggatcg gcggacaaag acttcaggct gaagagacgc gagaaactcg
     1321 agtacacggc ctcggcgttc atgctctatc tgggcgtcga tggaaagctc gagaacatgg
     1381 tgcaccacaa cttctacctt tccgggcgct acagggagaa cttcgaggcc atcttcagag
     1441 atcgcacgct accccaagac ccctcgttct acaccgtcgt cccttcgaag acggagccgc
     1501 gcatggctcc tgaggggatg gagagcctgt tcgtgctcgt ccccgtgccg caccttgggc
     1561 cgaacgtgga ctgggatcgc gacggggacg ctttcagaga gaaggtctac ggcctgctgg
     1621 agcggaggtg cggtctcgac cggagccgcg tcgtctacga gaaggcaagg acgccgctcg
     1681 actggcgaga cgagtacaac ttggaggagg gcgccgcgtt cggtatcggg cacggcatct
     1741 tccaggttgg gtacttccgg cctccgatgg tgtcgaaggc cgtacagggg atgtacttcg
     1801 tcggcgcgag caccaggccg ggtaccgggg tgccgctcgt taccataggg gcgaggctgg
     1861 tggccgaccg catagggcgg gagatcggcc tctcatgaag ctagaccctg ctggcggcaa
     1921 gaagctcgac ggcagtcttt cgctcgccga gagctacgag ctgtgccgca agattcagaa
     1981 ggctcacagc aggacctact atttctcgac caggctgttc cccgccgagg taaggccgcg
     2041 cgtgcacgcc ctctacgcct tcatgcgcta cgccgacgag atcgtagaca caccacacga
     2101 cctgccgctc gacgcccagc tctccgtcct cgaagagttc gaggccgaga ctatggcggc
     2161 cgtctccggt gagcaggtcc ccaaccccgt tctgcgcgcc tatgcggaca cggtgcttgc
     2221 ctgcggcata gagccagaga ccataaccgc ctttatggag agcatgaaga tggatacccg
     2281 cgtcttccgc taccagacat tctctgacct cgaggcatac acctacggga gcgccgccgt
     2341 cgtcggcttg atgatgtgca gggtcgtagg tgtcgccgaa gggaaggccg acacccacgc
     2401 cgaggccctg ggcatcgcca tgcagctctc caacttcctg cgcgacctcg gggaggactg
     2461 gcggcgcggg cgggtgtatc tgccgttgga ggatctggcg cacttcgggt atacggagca
     2521 ggacctcgct cttggtgtcg tcgacgaacg cttcgtcgca ctgatgcgct tcgagataga
     2581 cagggcgcgg cgactctacg aagtcgccga tggggggatt ggctacatcc cgagggggag
     2641 acgtttcccc gtggtcgtgg cgcggaagct gtatgcggcg atcctcgacc gcatcgaggc
     2701 ccagcgatac gacgtcttct cacgcagggc gcaggtctcg cgccccgcaa aactcctggt
     2761 cgccgccggg tgcgcagcca gcgaccccgg cgaaatagta gaccgcatcc gctacaggcg
     2821 gacccgccac acagacgcgc cagattgaaa tcgcctggct ttcgtgctct aattgcccgg
     2881 tcacatgccc catgagtggg ctatgacgct catcgagagc ggcggaggga cgggccctga
     2941 gaagccgcgg caaccggcga ggcgattgac cctcgcggcg ggtgccaact cccgcggagc
     3001 agattccggc agatgaggtg aaagatcaga cgcagcctct ctcctccgcg aatgtgtgac
     3061 gaataaggag agaggtcttt accttgagtc cgaacttgag agagcttctc gaggcgcgag
     3121 tcctgatcgg ggacggcgcc gtgggcacgc tgctcggcga acgcggcgtc ggcttcggcc
     3181 acccctacgc ccgcgcgaac ctctcccacc cagagatggt cacggatatc catgtggagt
     3241 atctgagggc tggcgccgac gccatagaga ccaacacttt cgccgccaac cgtttcaagc
     3301 tggagaccca cgacctccaa gagagggtgc gcgaagtaaa cgccgagggc gccaggctgg
     3361 cccgcaaggc ggcccgtacc gtcgcaggaa cgggggagag ggcgcttgtc ctcggggcca
     3421 tcggtccgct cggacgtccg ctcgtcccgg taggtcccgt cagtccagat agggcgaagt
     3481 acgtctttct ggagcaggcc gaagcgctcc tcgaaggcgg ggctgacgcg atcctcctgg
     3541 agaccttcac cgacctctcc gagctgcggc tcgcctacga ggcggtcgag accctgggcg
     3601 caccggtcct ggcgtacaag actttcgtcg aggacggcga gacgcttgcc gaagggcttc
     3661 cggagcgggc tgcgcgtgag atctcctcct ggggcgccga cctgacgggg gcgaactgta
     3721 ccgtcggtcc gcaaaggatg gtggagatca tcgggcagat ggccgtcggg gcaggtcccg
     3781 tcgcggcctt ccccaacccc gggctccccc agctcgtcag cggccacatc cgctttcaaa
     3841 gtgacgtcgc ccacttcgcc gagcacggcg tgaagctagc cgaggcaggg gcgcggctcg
     3901 taggcggctg ctgcgggacc accccggccc acgtgagggc gctctccgga gcgctgcggg
     3961 acttcagggt cgaaggcaaa ggcgcacgcc gccgggtgac cgtggtggag caggcaggac
     4021 ggacggaggt tgcggcagaa cccgtctctg agttcgcgga gcggttgcgg accggcttcg
     4081 ccgtgacggt cgagctcgac ctgccgcgcg gtaacgccat cgcgcaggtc gtcgaggccg
     4141 cgcgccggtt gaaggagcgt ggagtccacg ccatagacat ctccgacggg gcgcgggcca
     4201 ggctaaggat gcaccccgtg gcggcggcca ggatcgtaca ggaggagaca ggcatcgagg
     4261 tggtcgccca catctcatgc cgtgacagga acattatagg gttgcaagcc gacctgctcg
     4321 gtgctgctgc cctgggcgta aagaacatcc tggccgtgac gggcgaccca gcccagatcg
     4381 gggactaccc ggaagcgacc tcggtcttcg acaccgactc ggtcgggctc gtccacatcc
     4441 tctcaaggat gaaccggagg gaggacctcg cgggcaacca cataggcgag ccgccggggt
     4501 tcctcgtagg ggcgtctttc aaccctacgt cagaagacct ggcccaggag gtagagaagc
     4561 taaggcgcaa ggtcgaggct ggtgcccacg ccttctggac gcagcccgtc ttcgagttac
     4621 gggccctgga gaacgccctg gagcatattg gtgacaatgg ggtctgcatc ctgctcggcc
     4681 tcatgcccct ccgcagcgcc aagcaggcgg agttcctcca ccacgaggtc cccggtatac
     4741 acatccccaa gcacgttcgt gaaaacctcg ccaacctgcc ccccgaagac gccccgaaat
     4801 acggcgtcga ggtcgcccag aagctactcg tcgaggcgcg gcccctgata ggtggcgcct
     4861 acataatgcc gccggccagt gcgccagacc tcgcagggga tgtgctccag gcgataagct
     4921 ctccggtagg gagataggag tcgatggttc tccagagcac gaaaaacgaa gagaggcagg
     4981 ccaggttgta cgagttccgg gcactgctgg agcagcgcat tgtgctgctg gactgtgcca
     5041 tggggaccat gatccaatcc tacgggctct ccgaagggga ctacaggggg aaacggttcg
     5101 ccgaccatcc aggagagcaa aagggcaaca acgacctgct ctcgatcacc cagccaggga
     5161 taatcagcga catccacgaa gcggcgatgg acgcgggatc ggacatcatc gagacgaaca
     5221 ccttcaactc tacctccatc gcccagacgg agtaccggct ggaggacctg gcctacgacc
     5281 tgaactacga ggcggcctgc gtggcccgcg aggctgctga cgcctacact aagaagacgc
     5341 tacagaagct gcgctttgtg gcgggcgccc tcgggccgac gaaccgcacg gcctcgctct
     5401 cgcctgatgt caacaatccc ggcttcagga acatcacctt cgacgagcta gccaaatcct
     5461 acgcagaggc cgcgcggggg ctcgtggagg ggggggttga cctgctgcta atagagaccg
     5521 tcttcgacac attgaacgcc agggccgcca tcttcgggat cacgtcttac ctcgaagagc
     5581 agggtctcga cgttcccatc atgatctcgg gcacgatcac cgacgctagc gggcgcacgc
     5641 tctcggggca ggttaccgaa gctttctgga actccgtgcg ccacgcgaac cccataagcg
     5701 tggggctgaa ttgcgcgctc ggatcgaaag agctgaggca gtacatcggg gaactctccc
     5761 ggatctcgga agttcccatt agtgcctatc cgaacgcagg actgcccaac gagttcggcg
     5821 agtacgaaga gacgccggag atgatggcgg ccgagatccg ggagtgggca gaggccggct
     5881 ggctgaacat cgtcggcggc tgctgcggga cgacgccgga tcacttgagg gccatagccg
     5941 aggcggtctc tggccacccg ccgcgtgaga taccggagat acccaagaag ctcagacttt
     6001 cggggctcga gccgtgcaac atcgggcccg actcgttgtt cgtgaacgtc ggcgaacgga
     6061 ccaacgtcac gggttcggcg cggttcaagg acctcatatc gagcgaagac tacgagacgg
     6121 ccctggacgt ggcgcgccag caggtcgaga acggcgccca gatcatagac atcaacatgg
     6181 acgaggggat gctcgaaggt gaggagtcga tggtccgttt cctcgacctc gtggcctcag
     6241 agcccgacat ctgccgggta cccgtcatga tcgactcctc caggtggtcg atcatcgagg
     6301 ccggcctgaa acgggtgcag ggcaaggcgg tggtcaactc tataagcctg aaggagggcg
     6361 aggaggagtt cgtcaggcag gcgcgacttc tgaagcggta cggtgctgcg gtgatcgtta
     6421 tggccttcga cgagaagggc caggccgaaa caaaggagcg gaaggtcgag atatgcagac
     6481 gcgcctacgg cgtcctcaca gagggggtcg ggtttccgcc cgaggacatc atcttggacc
     6541 ccaacgtctt cgccatcgcc acaggcatcg aggagcacga caactacggt gtcgacttta
     6601 tcgaggctgt gcgtgagatc tcctctaccc tcccccacgc caagacctca ggcggcatct
     6661 cgaacgtctc gttctcgttc aggggcaaca acccgatgcg ggaggccatc cacgcggtct
     6721 tcctctacca cgccataaag gcaggcctga cgatgggcat agtgaacgcg ggccagctcg
     6781 ccgtctacga cgagattgaa gaagacctgc gcgaggccgt cgaggacgtt gtcctgaacc
     6841 gccgtcccga ctctaccgaa ggcctgcttg ccctggcgca gaggtaccgc gaccgtggcg
     6901 actcggtaac ggacgagcag gagagagaat ggaggggctg gcctgtcgag aagaggctcg
     6961 agcacgcgtt ggtcaagggc atagccgagt tcgtcgagga ggacgcggag gaggcgcgcc
     7021 tccaggccgg gcgtccgctc gatgtgatcg aaggcccgct tatgcacggg atgaacgtcg
     7081 tcggggacct gttcggatct ggcaggatgt tcctgccgca ggtcgtaaag agcgcccgcg
     7141 tgatgaagaa ggccgtcgcc tacctcatcc cgtttatgga ggagaacggg gatgaggcac
     7201 gcaagcctaa cgggacggtc gttatggcaa cagtaaaagg cgatgtgcac gacatcggga
     7261 agaacatcgt cggggtcgtg ctgcagtgta acaacttcga ggtcatagac ctcggcgtaa
     7321 tggttccttc ggcgaagatc cttcagaccg ccagtgagaa gggcgctgac gccatcgggc
     7381 tctccggcct cataactccc tctctggacg agatggtcca caatgctaaa gagatgcagc
     7441 gcgaaggctt cgacataccg ttgctcatcg gcggggctac gacctcccag atgcacacgg
     7501 ccgtcaagat cgcacctgga tacgaacagc ccgtgatcca cgtgaaggac gcctcgcgcg
     7561 ccgtcggcgt ggtccagaac ctggtaagcg aaggccgcaa ggtcgactac gccgagcaga
     7621 tcgccgccga ctacgagcgg gtccggcgca agcacgaagg acgccgctcg aagaccaccc
     7681 tcgcgacgct ggagagtgcg cgcgccaacc gcacgaagat cgactgggaa ggctactccc
     7741 cgccgaagcc caacgttcta ggcgtacaga cgttcgacga ctacgatctg cgggaaataa
     7801 agccctacat agactggacg cccttcttcc actcctggca actcaagggc agccatccca
     7861 ggatacttga cgaccccgag aaaggcgaag aggccagaac gctcttcgcc gacgcgcaag
     7921 agctactgga ccgcatcata accgagaagt ggctcacggc ccgcgccgtc ttcggcctct
     7981 tccccgcgaa cgccctggaa gacgacaccc tcgaggtcta caccgacgaa tcgcgcgaag
     8041 aggtgctcgc cagcctcgac ttcctgcgcc agcagaaaca gaagccgcct ggccgtccga
     8101 accgctccct cgccgacttc gtagccccca ggagcatggg tctggaagac tacgtagggc
     8161 tcttcgccgt gaccgcgggc ctcggtgccg acgaggcggc gcggcgcttc gaggaggaca
     8221 acgacgatta caacgcgatc atggtcaagg cgctggccga caggctcgcc gaggccttcg
     8281 ccgagctcat gcaccggcgg gtacgtacgg agttctgggg ttacgcagcg gacgaggatc
     8341 tggacaacga ggccctgatc agggaggagt atggcgggat ccgcccggcc ccaggatatc
     8401 ctgcctgccc ggagcacaca gagaagcgga ccctttggag attgctcgac gtggggaaca
     8461 atgctggcat cacgctgacc gagagctgcg cgatgtcgcc aggcgcggcc gtcagcggct
     8521 actacttctc acaccctgat tgcaggtact tcggcgtagc cgagatcgga cgcgaccagg
     8581 tcgaagacta cgccgaacgc aaaggctgga ccctggaaga ggccgagaag tggctctccc
     8641 ccaacctcgg ctacgacgtc gagcataagg cttgagaacc tgagcatgtc acccgtcgag
     8701 ggtttcttcc ctctctgacc tgagcctggg atcggtaccg gggacggagc gccggatcag
     8761 acgctccacc cgctcgaaga agcgggtgcg atccagtaga gggtttccag aaccccgcgg
     8821 tgatgcagta gtaggtgtcg taggggctct cgtgatggat gtcgtggtgc tccctggaga
     8881 ggatcgcccc acgcctggag ccaacctaca aaggccggcg ggatatccat gtgcgcccac
     8941 ttgtggaact ggttggtaag gaaggcggcg aggcacaaga aaagagagaa ggtcccgaag
     9001 agatacccat agtaagtagt ctccaaaggc acgataatcc acacaccgag catgaaggga
     9061 aggctcgcca ggctgttgtt gccgttggcg tcaacgaagt cgttgccgac gatccctttc
     9121 gggtcgatgt ggtgctcccg gaagggttcg atgaaattgg gtccgatgat cggcgtgtca
     9181 taggagccga agttgtcggc gaggaagtgt acgaacccgg agaggaagtc agcggccagg
     9241 taagccagga cagccaggat cggcgcgagc cacaggtctc cgaagtcgat catcccctgg
     9301 tagacatcca cgcctatcaa caacaaaagc aacacgaagc ataaaatgcc aaggacttcc
     9361 agcaaataat ggcctgtgct ggtctcggga cggatctggt cttgcgtagc gtggtcttgc
     9421 tgggtcatat acaacctcct tggtggggca tacggaaagg gatggtctct ggatcagtgc
     9481 ggaagagaac tcttgccgac tcacctgcgg gcaggtgaca ctcggagtgc cccaaaccta
     9541 ggaatcttcg ggcatacagc cggttgcttt gaggtcttag cggaacctga cctcttcgaa
     9601 tcctagagta gtgacgaacg ctcgtacgct atcttctgcg ttgtgttcgg ctgtaactag
     9661 aatcccgttc tctcgcgcgg catctctgat cttcttcacc gcttgcagac gggcttcttc
     9721 gacgaggtcg tcgtcggggc ggacgttcag ggggctgaag tcgcggtcgt agacgcgggt
     9781 tttctcctcg tcgaggctgg cagagaggat ctggggttca ggtagatcta tggtgacggt
     9841 gtcgctgttc acgctgacat cctccttgcc gatgtcacca aggtcaaccc cggcctcgac
     9901 ttctcctgtg gcgatcacca gcaccctctc cccgtcgaac aggcggtcca gaatatcgcc
     9961 gccggtctcc ctcgtcacag gcacggactc ggtccatcgc accgtggcga gctggtcgag
    10021 ctctcttatc ccttccacca ccacaggccc cgtcgtcgta cgcggcggct tctctccgag
    10081 aagcggcccc actatcggaa gcaacaagcc atagcgcgcc agcccgacgc cgagcgcgag
    10141 gctcaagacg acgataacga gcgccagaac gagcgtagtt ccgagcccgc cacctttatt
    10201 gcccatgttc tccctttttc cgagcgtgca gttgcggaga ttctacaccg gtcgaaatcc
    10261 ctgtcgggcc tctacactac ccagcggata agaagctgac tggctgaaat gctgtcacgc
    10321 ctgaatattc gtctcgcgcc agcgggtgtg cccgccgagg cccccggcct cagggaactc
    10381 ctcgcccttg gagagctgta cccggacagg cgggagcatg gtccctctgt cgcagatgta
    10441 ggtgcccgag ataggggcct tctcgccggg cctgaaggtt ggtgcgtagg gttcgtagcg
    10501 ggagagtatg tcttccgggc cggccatcac agggtgaccg agcgggcctc gttgaaaccc
    10561 ggcgtctcta cgagggcgtt caagacttct tcggggacgg gctcgtccac ggtgacggcc
    10621 atcgccgcgc gggaaccagg ctcgccgcgc ccgacggcca tgttgccgat gttgatgccg
    10681 tgttccccga ggatactgcc gagcttcccg atcatgccag gcctgtcctc gttacggatg
    10741 aagagcatgt acttctcggg caccacgtcg aagtcgaatc ccatcgcttc gacgagccgc
    10801 ggctgcatcc ttggcccgaa gagcgtcccg ctcaccgcgt tctctcccat gcgcaactcc
    10861 acgaggttcg tgtagtccgc gctctccgaa gtccgcgagg tctcgacctt gagcccgcgc
//
LOCUS       DASTKM010000101        18684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_375543, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000101 DASTKM010000000
VERSION     DASTKM010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 18684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18684
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375543"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..467)
                     /locus_tag="VFQ10_04095"
     CDS             complement(87..467)
                     /locus_tag="VFQ10_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET9964554.1"
                     /translation="MQPHNEHREPQEHGGTEIREHLANERTLLSWVRTGVSLISIGLV
                     VERAAALVDASMKVGSTSASELFGLALALLGALTLVIGTTQFLNNRRRISTGEFVPSV
                     AAYLIIVAGSLAFAGAFVIYVLIT"
     gene            633..1949
                     /locus_tag="VFQ10_04100"
     CDS             633..1949
                     /locus_tag="VFQ10_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET9964555.1"
                     /translation="MSKGDRLLDRRAGLEQEFFLLENSGHPSERADEFLERCREESAG
                     PVCFAPEFVLGLVEVNTPPVYTLYDLEREYAQNLRIALRTARSLGLRLYPLGTYPLPL
                     EPAVRDGLDYRVQVGTVGPERFVDAGRCAGTHLHLELQAGTVDEGVGISATASAAARE
                     EVSNIYNLATALDPALVALTRSCPFFEGHKTGLAVRTVHYRGSANFGWEGVYRDLPQV
                     GALLPYAEDAEHLIRQQFDRYKAWLSAMDRAGVKRRHFAEAGGDLLRPAWNPVRLNRQ
                     GTLELRGMDSNYPEVTLTVAAIILRAADRVRRDGLEVSPDDGVRAFELTGDVLRVPGF
                     GYLGGELLHAAVTGGAGDETVAAYLDSILEFAGVEDERLAVLLHHRRTTGYYPTTEAG
                     ILEAYDGHLSEDEGLRLVLEACDELETQVSHLSLSQRQEEEPADVS"
     gene            1984..2475
                     /locus_tag="VFQ10_04105"
     CDS             1984..2475
                     /locus_tag="VFQ10_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9964556.1"
                     /translation="MGKDRAALSMDFGVRVAAVVEREGALLLVRHQKPDRDPYWVLPG
                     GRLEPGERIPECAGRELAEETGLTAGFSGVLYVSEFLRDGRHTIDVVARMALEGDEEA
                     TLGSDPEVEPGAEPTLREIRWVSVEELQEIELLPASIKERLLRDAAGGWAPENVYLGS
                     TDG"
     gene            2468..3190
                     /locus_tag="VFQ10_04110"
     CDS             2468..3190
                     /locus_tag="VFQ10_04110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9964557.1"
                     /translation="MADPQDTLLSVVLLDAAVAGIVIGGFWLAVTWMRSLGKKIGYSL
                     APLGFSRPRGGLLAGIGAGMAVGIGAFLLSIIVTPLSVLVLERLGYSTRSTVQQPFME
                     GLVGWVQESPGVAVPAIILVVVLFGPAVEELVFRGVVFNGLYRLGTLVYTRSVGTEHS
                     KRPPGKTIFVSSALVSSVLFALLHLEPVLLPVLLILAITLCALFQRTGSLLPSFVAHA
                     TFNSVAAMLIILRGMNVLNIPI"
     gene            complement(3187..4410)
                     /locus_tag="VFQ10_04115"
     CDS             complement(3187..4410)
                     /locus_tag="VFQ10_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET9964558.1"
                     /translation="MREERYLDAGRAAEELGVSLATLYAYVSRGMVRSEAVEGKGRNR
                     RYRAEDVRRLKERKERRRDPEGVVEGALHWGTPVLESAITMIDDGGGLYYRGRDVAAL
                     AGEKSIEKVAALIWTGDEAMAPALFPPEMSGPSWRISDVVDSVAGLTPVEVFQVMLPV
                     AAADDPAAYDLRPGAVARTGARILRLMTDVAAGESAPGLAATLQRGWSPDDPEAADLL
                     GSALVFCVDHELPVSTFAARCVASSEATPYAVVLAGLAALGGVKHGGEIELVEAFLRE
                     VDASGDARAVISGRLRRGERIPGFGHSLYPEGDPRGAGLLRLTAGVYPESIAVTLSDS
                     VAGEMLQLMGERPTVDFALATVARALELPPGGAVALFALGRTVGWIGHAIEQYRSGSL
                     IRPRARYVGEQPGER"
     gene            4564..5694
                     /locus_tag="VFQ10_04120"
     CDS             4564..5694
                     /locus_tag="VFQ10_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HET9964559.1"
                     /translation="MGESSGTGFVAGLEDVVAAETRLSGVDGEAGELIIAGFQVEELA
                     GRASFEETVYLLWHDILPTSGQLADFREEVAGRRELHPATLDLLRAVAAEKVPAMDAL
                     RMAAGTVSPGTDYEEALALVARLPTIVTAYWRMLDGEEPVDPDPELGHAANYLYMLTG
                     EVPGAEFARALDTYLSTVSDHGMNASTFAARVIVATRSDFVSAVVGAIGALKGPLHGG
                     APGPALDVVFEIGDANRAEAYLREKLGRGERLMGFGHRIYRVRDPRADVLAAAAERLY
                     TTDGDRELYELALDVEQTALTLLAEHKPGRNLQTNVEFYTALLLHGLRLPTQIFTPTF
                     AVGRVAGWTAHCFEQRALDRLIRPQSVYVGSRDRSWVALEER"
     gene            complement(6450..7718)
                     /locus_tag="VFQ10_04125"
     CDS             complement(6450..7718)
                     /locus_tag="VFQ10_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET9964560.1"
                     /translation="MPDGKNIERAEIVTVGTEMLLGDLVDTNTAWISQRLAGLGVGIY
                     RHTTVGDNSERIVAALREAASSSDLVVTTGGLGPTSDDLTNACLSTLTGREMVEYPEA
                     REHVDTMFTKFGRKPTANNYKQALFPQGTELIPNPVGTAMGALVEWEGTLFATLPGVP
                     SEMTSMFEATLEPLIRARSEGSIVSKTLHFAGIGESALAEKVQEFLDATDPTVAPLAG
                     QGKVRLRITTRAATEKEAQEKIAPVEKELIARLSGYYFGEDNETLEGAVGRLLQERGA
                     TLALAESCTGGLLAKRLTDMPGSSAFFGEGLVTYSNESKERLLGVPHALIMEHGAVSE
                     PVAREMAEGARRISGADYGLSVTGIAGPDGGTEEKPVGLVFVGISDSEDTFAKKLDLT
                     AWARSRDSIRERSANQAFDLLRLRLEENQS"
     gene            7742..8353
                     /gene="yqeK"
                     /locus_tag="VFQ10_04130"
     CDS             7742..8353
                     /gene="yqeK"
                     /locus_tag="VFQ10_04130"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET9964561.1"
                     /translation="MEDHAKTHTLLERAEAFARSRLSERRYEHTLRVADTAESLALAH
                     DLEADRARLSALLHDAAREKEPEEFLKLADEWNLQVGDPERQSPKLLHGSVAAELARR
                     ELGIDDEEVLEAVRAHTTGSPGMGPLALVLYVADKIEPARDYPSVGRLRTLAREDLRR
                     AAEESLRRAIAHNEERGKATHPASLRMLDWLEADRTAQSRIEK"
     gene            complement(8303..8872)
                     /locus_tag="VFQ10_04135"
     CDS             complement(8303..8872)
                     /locus_tag="VFQ10_04135"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET9964562.1"
                     /translation="MNATTHAVFGIGALAGAALVTGADPPAYVYPAAVAAAWLPDIDN
                     PRSTLGNGLNRLKNPTLNLLSRPLSWALRTASFVLVRTVGHRTMTHSVFGVLLFSLLV
                     LLFLGRFPNLALALVAGYASHVVADALNTRGVPLLWPIGKPFRILPGGVRSGGAVEVA
                     VALVALAFTLYALLLLYPGLRGAIGLQPI"
     gene            complement(8904..9359)
                     /gene="dut"
                     /locus_tag="VFQ10_04140"
     CDS             complement(8904..9359)
                     /gene="dut"
                     /locus_tag="VFQ10_04140"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET9964563.1"
                     /translation="MSSERPLQVPFRRLVPEAGMPERAHLGDAGYDLRSVERVDLAPG
                     SRALVRTGISVAIPEGYAGLVLPRSGLAARHGISLVNTPGLIDSGYRGEIQVPLINHD
                     RQETFLIEEGTRIAQLVLVRVASAFFAEVEFLESATDGRGEGGFGSSGS"
     gene            9500..11224
                     /gene="metG"
                     /locus_tag="VFQ10_04145"
     CDS             9500..11224
                     /gene="metG"
                     /locus_tag="VFQ10_04145"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET9964564.1"
                     /translation="MSEQRRRYLVTSALPYANGPLHVGQISGAYLPADAFVRYLRARG
                     EDVVYACGTDEHGVPITLTAEREGKDPQEVVDYWWVNMKETFQRFSISFDNFSRTSTP
                     LHAKNTQLFYRKLKEGGYISEAESKQMYSPTEGRFLPDRYVEGTCPVCGYKEARGDQC
                     DNCGSWYEAYELIEPHSKVTGGPVEVRETTHLYLDLPEFSDRLREYVAGQEHWRTSVK
                     NFILGIIDGGLEKRPITRDIQWGVPVPEPGFEDKRFYVWFDACIGYVSSTMEWAQNIG
                     EPDRWREYWQEPESRLIHFIGKDNTVFHTLIWPAQLMGTAEDGEEPFVLPYDVPANEF
                     MNLEVLVDGERRAMQMSTSRNLAVWLHEALDRFPADPLRFYLSSNLPETGDVVFSWRD
                     FQSRVNSDLIGNLGNYINRVLSFAERYFDGEVPRPEELPEDAVAVLEDFRELEARYEA
                     KMLAPRPREALGELLAMGRSANRFFDASAPWKTRKEDPEQTRLTLYTCSVLLGSLAYH
                     AAPYVPEAIGRLEEFFDGPIARVSDLDDLPKGYRAAGAKPLFRRIEDDEVAAAEGQLS
                     RAVRGEAS"
     gene            complement(11261..12196)
                     /gene="thyX"
                     /locus_tag="VFQ10_04150"
     CDS             complement(11261..12196)
                     /gene="thyX"
                     /locus_tag="VFQ10_04150"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET9964565.1"
                     /translation="METTERNTERARQLFEELGGAVKDGFPNIHSQVHHTKAGTPYLK
                     RPGVVVLAKPQTNVAGLGVFLEGFDPDLHFTEYLDDPTRLPDSSQLCKTAGQTCYMSF
                     GPRRTKNDNAAAYIERLTSAGHGSVLEHSTFNFLLYGISRSVTHELVRHRAGTAFSQI
                     SQRYVSGAVLRFVERPEYQEDEELHRAFEERADRAATEYEAMAELLLERQEGGASMLT
                     ADYRTDARKKVQQTARSLLPNETEAPMVFTGNVRALRHIIEMRADAHAESEIRNLALR
                     IFLCLRTADPILFGDYELGELPDGTYTVSTKNRKA"
     gene            complement(12252..13094)
                     /locus_tag="VFQ10_04155"
     CDS             complement(12252..13094)
                     /locus_tag="VFQ10_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HET9964566.1"
                     /translation="MMEAQDVLTQYLGRVRGSRLLDADEEKALARRVRGGDMRARQKL
                     IESNLRLVISIAKKYRGRGVLFEDLIQEGNAGLIRAVEKFDPEMGNRFSTYATWWIRQ
                     AVTRAVADHARTVRLPAHVVDALYRLRRAENALSIELGRDATEEELVERLGVKPEEAR
                     RLREVSQPISSINAKINAEEGSEFGELLPDERSGDDYARVEVGQWERVLVEAVRSLPE
                     REARIIEMRHGLDGSETLTLREVSEELGISQERARQVEIKALRTIRTGRYASTLRRAL
                     SEAV"
     gene            complement(13177..13473)
                     /locus_tag="VFQ10_04160"
     CDS             complement(13177..13473)
                     /locus_tag="VFQ10_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964567.1"
                     /translation="MSGEAKRKARKRRSALRPDTALVPVGEPEQEIYVWSLPTCCLRS
                     LKETAMSEVGERRLLELDCSCGRRWHITSSLDPRILERFVTHGGPRVGGSYPAA"
     gene            complement(13517..13720)
                     /locus_tag="VFQ10_04165"
     CDS             complement(13517..13720)
                     /locus_tag="VFQ10_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964568.1"
                     /translation="MGGSAWIAALGEEVVYEEDLPACCRRSALHYDDSFECPVCGAMW
                     REPLPVQPEEDAFAQREERRGAA"
     gene            complement(13720..14292)
                     /locus_tag="VFQ10_04170"
     CDS             complement(13720..14292)
                     /locus_tag="VFQ10_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964569.1"
                     /translation="MREEYMIERQGKRFVLYAGLLEEAHSRGLRSIETELLQVPAKEN
                     GEVAIVKAVIRTEEGKFAGIGDASPQNVNRTIAPHLIRMAETRAKARALRDAINVGVT
                     AFEELGGEEEVVEASGTQEREGRPRAQNEEGRGELPATRKQLNYLEALISDVVEDGIG
                     RFEEMVGKPVGELTREEASEWIGRLSGRAA"
     gene            complement(14369..14941)
                     /locus_tag="VFQ10_04175"
     CDS             complement(14369..14941)
                     /locus_tag="VFQ10_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9964570.1"
                     /translation="MIEERAHQVFEVAGGDFGGKLMVTRRTGRAVREALEGALEGLPK
                     GGILYVDTRGVELMDYSFADEALGILASRSASGEYGDKHLVLVEEDRDLLENVEASLR
                     QRSLAMIRVDEIGAEPGIVGKVPDHLVETLQAIYDAGSITNAALAARLDLNHTACNNR
                     ATRLAKLGLIHRRIETAAPGGRQYTYERIA"
     gene            complement(15036..15950)
                     /locus_tag="VFQ10_04180"
     CDS             complement(15036..15950)
                     /locus_tag="VFQ10_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964571.1"
                     /translation="MKTQLELNIRSRGAMSGVLTMGSLDPAFAAATSVQGEPVVVDLR
                     TVEFVEPAGLCALAALLEYLSARCEVVDLALAGRDVPAYLERMNFFRSFGETVRTNAD
                     VASLEERRRHNPGTLQELINFHTEEEIPGIIERISEILENQGYRLRERVAICSTLSEV
                     CANAAEHGVSSFGAYAAVQAYHHIVSGSRRRGEEVLIAIADGGVGVRETLTRNPEYAE
                     HTTTDNDALRHALEMGVSATGEIGRGGGLALVAGIAARAGGSLSLRSGSGRVTVYENR
                     KNSRNVPDFPGTFVRVSLPRTPQEKTSG"
     gene            complement(16185..16682)
                     /locus_tag="VFQ10_04185"
     CDS             complement(16185..16682)
                     /locus_tag="VFQ10_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9964572.1"
                     /translation="MILRPPRESDVEVVYSWDRDPELAAWNGRSPISISLSAARRDYL
                     ARWEDPSVRTFIIEARGEPIGMATLYEFRRDGCELGIKIGPGNLRGRGYATEAVTLLI
                     DYVFHTLGLAAVRGSTLAHNDRMQRVFEKNGFVRVGDGSLLSRYDNRRYTELFYERRR
                     EDHKP"
     gene            16834..18159
                     /locus_tag="VFQ10_04190"
     CDS             16834..18159
                     /locus_tag="VFQ10_04190"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964573.1"
                     /translation="MSGTVGELFGGSSRRVRDVIWGDVSIDRAVKTLLETGAMQRLRG
                     MSQLGFTFGSFPRARHTRLDHAVGVYHLAGLTLKRIAESGAYLEDRDVRAALAAALLL
                     DAGRYPYAASVEGIKVPGAPTLKELSRRWIEHTEVAKVLRAEWDLEPHNVFRLVSRGD
                     TPPHSLTPTEHLLRDILFGSLNVDALDRLIRDARGARVPYGLVEVESLIQRLRIVGQE
                     NKAILALDEEGAGTLQSVVFSRYLVYYNVYGHHALRIPTVMFLRAVQDAIGAGNVTFD
                     ELSELDDAGAFVLLEDSAESSTAALMKRLADRRPYHLALEFDERHPSYSSLIRLRDDA
                     SWRRRVEEAWSRYLTRYRKGVAGPFDILIDLPAREHFPVELRLIRHNPLPGERNPIGW
                     HNLSGMSEDEMTRYHAPLRRILIATANDDLVKSVRRHAEELFTIAEEVG"
     gene            18556..18628
                     /locus_tag="VFQ10_04195"
     tRNA            18556..18628
                     /locus_tag="VFQ10_04195"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18589..18591,aa:Lys,seq:ctt)
BASE COUNT         3403 a         5907 c         5808 g         3566 t
ORIGIN      
        1 tacgtttggg tgggaagcca ggtcggaacc aggaacgtgg cctccgggaa gccttgatat
       61 tcggcttcag atttctctgg cgggggctac gtgatgagca cgtatatgac gaacgccccg
      121 gcgaaggcca ggctacctgc gactattatc aggtacgctg ctactgatgg aacgaactcg
      181 cccgtggaga tcctgcgccg gttattgagg aactgggtgg tacctatgac gagcgtgagc
      241 gccccgagta gggccagcgc cagcccgaag agctccgagg cgctcgtgga gccaaccttc
      301 atggaagcat ccacaagcgc cgccgcccgc tcgacgacca gccctatgga gatcaggctt
      361 acccccgtcc tcacccacga gagcagcgtc cgctcgttcg cgaggtgctc cctgatctcg
      421 gtcccgccat gctcctgtgg ctcgcggtgc tcgttgtgcg gttgcaagat ctctgacctc
      481 cttctcgtcc acaccgaaga ttacatgttg gagtgaccga gagattcctc gctgcccgga
      541 tttccgcccc agggccaaag cctgctcccc acatctaggg tagcgtttcc cccacaaaag
      601 atagagaata cgtcaaggca ttcggggaca gggtgagtaa aggcgacagg ttgttggatc
      661 gacgggccgg gctcgagcaa gaattcttcc tgctcgagaa ttccgggcat ccatcggagc
      721 gggccgacga gtttctggag cgctgccgcg aggaatctgc cggtcctgtg tgcttcgccc
      781 ccgagttcgt gttgggcctg gtcgaggtaa acacgcctcc tgtctacacc ctctatgacc
      841 tggagcgtga gtacgcgcag aacctgcgga ttgccctgcg cacggcgaga tctctgggtc
      901 tcaggctcta cccgctcggc acctatcctt tgcctctgga gccagccgtc cgcgacgggc
      961 tggactacag ggtccaggtg ggcacggtcg gacccgagag gttcgtggac gccggcaggt
     1021 gcgcgggcac gcacctgcat ctggagttgc aggcggggac ggtcgatgag ggagtaggaa
     1081 tctccgccac cgcgtcagcc gccgcacggg aggaagtctc gaacatctac aacctcgcga
     1141 cggccctgga tcccgctctg gtggccctca cccgctcgtg tccattcttc gaggggcaca
     1201 agacggggct ggccgtccgc accgtccatt acaggggcag cgccaacttc gggtgggaag
     1261 gagtctatag ggacctgccc caggttgggg ccttgctacc ctacgccgaa gacgcagaac
     1321 acctgatccg tcagcagttc gaccgctaca aagcctggct cagcgctatg gaccgggccg
     1381 gcgtaaagag acgccacttc gccgaggcgg gcggagacct gctcaggccg gcctggaacc
     1441 ccgtgcgtct caaccgccag ggaacgctgg agttacgcgg catggatagc aactaccccg
     1501 aagtaacgct gactgtggcg gccattatcc tccgcgccgc ggaccgcgtc aggcgggatg
     1561 gcctcgaagt atcgccggac gacggggttc gcgctttcga gcttacggga gacgtgctga
     1621 gggtgcctgg cttcggttat ctcggcgggg agctgctcca cgcggcggtt accggaggag
     1681 cgggtgacga gaccgtagcc gcctacctgg actcgatcct ggagttcgcc ggcgtagagg
     1741 acgaaaggct cgccgtcctc cttcaccacc gccgtacgac cggatattac ccaaccaccg
     1801 aggccggtat cctcgaagcc tacgacggac atctctccga agacgaaggt ttgcgcctgg
     1861 tgctcgaagc atgcgacgag ttggagaccc aggtctcgca cttgagcctg tctcagcgcc
     1921 aggaggaaga gccggcggac gtctcgtagc tctccctggt cttctcgcgg gtatactcgc
     1981 tgcatgggca aggatcgtgc agctctcagt atggacttcg gggtgcgggt cgcagcagtc
     2041 gtggagcggg agggagcact gctcctggta cgccaccaga aaccggacag ggacccgtac
     2101 tgggtgctgc caggaggcag gctcgagccg ggcgagagga taccggagtg cgcaggccgc
     2161 gaactcgctg aggagactgg ccttacggcc ggcttctccg gtgtcctcta cgtgagcgag
     2221 ttcctgaggg atggacgcca tacgatcgac gtcgtcgcca ggatggcgct ggaaggagac
     2281 gaggaagcca ccctcgggag cgaccccgag gtcgaacctg gtgccgagcc gaccctcaga
     2341 gagatacgct gggtgagcgt ggaggaactg caggagatcg agcttctacc ggcttccata
     2401 aaggaaaggc tcctgaggga cgccgccggc ggatgggccc cggagaacgt ctacctcgga
     2461 agcacggatg gctgacccgc aggacacgtt gctctcggtc gttttgttgg acgcggccgt
     2521 cgcggggatc gtgatcgggg ggttctggct ggcagtcact tggatgcgga gcttgggcaa
     2581 gaagatagga tactctctgg cgccccttgg attctcgcgg cccaggggag ggctcctggc
     2641 gggaatcggc gccgggatgg ccgtcggcat cggagccttc ctcctcagta taatcgtaac
     2701 ccctctcagc gtcctcgtcc tcgaacggct cggctactct acgagatcga cggtccagca
     2761 accgttcatg gaaggactgg ttggctgggt acaggagagt ccgggcgtgg ccgtaccggc
     2821 gataatcctg gtcgtcgtac tcttcggacc ggccgtcgag gaacttgtat tcaggggtgt
     2881 tgtcttcaac ggcctctacc ggctgggaac ccttgtctac accagatccg ttgggacgga
     2941 gcattccaaa aggcctcccg gtaagacgat cttcgtctct tccgcactcg tatcttctgt
     3001 actattcgcc ctgctgcacc tggagccagt cttgttaccc gtactcttga tacttgcgat
     3061 aactttgtgc gctctgttcc agcgtacggg tagcctcctt ccctcgttcg tcgcgcacgc
     3121 gacgttcaac tccgtcgccg ccatgcttat catcctgaga ggcatgaacg tcctgaacat
     3181 tcccatctag cgctcgcccg gctgttctcc gacgtatctt gccctggggc gtatgaggga
     3241 gccgctccgg tactgctcta tggcgtggcc gatccaacct acggtcctgc cgagggcgaa
     3301 gagcgcgacg gccccgccag gcggcaactc cagagcccgc gccaccgtcg ccagggcgaa
     3361 atccacggtg ggacgctcgc ccatcaattg cagcatctct ccggcaacac tatcggagag
     3421 cgtgacggcg atactctcag gatacacccc ggccgtcaga cgaagtaatc cggcgccgcg
     3481 gggatcccct tcaggataaa gcgagtgccc gaagccaggt atacgctccc ctctcctgag
     3541 acgcccggag atcaccgccc gcgcatcccc cgaggcatcc acctcccgca aaaaggcctc
     3601 cacgagctcg atctctcccc cgtgcttgac gcccccaagc gccgcaagcc cggccagaac
     3661 cacggcatag ggcgtggcct cggacgaggc gacacacctg gcagcgaagg tcgagacggg
     3721 taactcatgg tcgacacaaa agacgagcgc ggagccgaga aggtcggcag cctctggatc
     3781 gtctggactc cagccgcgct ggagcgtcgc ggccagacca ggagcggact ctccagcggc
     3841 cacgtccgtc atcaggcgga gtatcctagc gccggtgcgc gcgacagcgc caggacgcag
     3901 gtcgtatgcc gcagggtcat cggcggccgc cacagggagc atgacctgga agacttctac
     3961 aggcgttagt ccggcaacgc tgtctacaac gtcacttatg cgccaggagg gtcctgacat
     4021 ctcgggtggg aagagcgccg gggccatggc ctcgtcgccc gtccagatca aagccgcaac
     4081 cttctcgatg ctcttctccc cggcgagtgc agcgacgtcg cgccccctgt agtacaatcc
     4141 gccaccgtcg tcgatcatgg tgatcgctga ttccaacaca ggcgtgcccc agtgcagggc
     4201 accctcgaca accccctcag ggtcccgcct gcgctccttg cgctccttga gcctccttac
     4261 gtcctcagcc cggtaacgcc tgttgcgccc ctttccctcg accgcctcgg aacgtaccat
     4321 gcccctgcta acgtacgcat acaaagtcgc cagactgacc cccaactcct ccgcagcccg
     4381 cccagcgtcg agataccgct cctccctcat ccaacagcct cctcgcccgg gtgtacgacc
     4441 atgagcccga aaccctacta tacattgatt atcgcatcaa tattgataca tgttttatag
     4501 agagttacga ttgacagata tgacggcggc tcggggatcc gggtcaggca tgaaaggact
     4561 gacatgggcg agagttctgg gacgggtttc gttgcggggt tggaggacgt ggtggcggcc
     4621 gagacccgcc tgagcggtgt ggacggagag gctggggagt tgatcattgc gggtttccag
     4681 gtcgaggagc tggctggacg agcttccttc gaggagaccg tttacctgct ctggcacgat
     4741 atcctgccga cttcgggtca gctcgcggat tttcgagagg aggtcgccgg gaggcgggag
     4801 ttgcatccgg ctacgttgga cctactgagg gccgtggctg cggagaaggt accggcgatg
     4861 gatgcgctgc gcatggctgc cggtaccgtg agcccgggca cggactatga ggaggctctg
     4921 gcgctggtgg cgcgtctacc cactattgtg acggcgtact ggcggatgct cgatggtgag
     4981 gagcccgtgg accctgatcc tgaactaggc catgctgcca actacctgta catgctcacg
     5041 ggcgaagtgc ctggcgcgga gttcgcgcgg gccctcgata cgtacctcag tactgtgtcc
     5101 gaccacggca tgaacgcctc cacattcgcg gcgagggtca tcgtcgctac acgctcggac
     5161 ttcgtctccg ctgtggtcgg ggcgatcggg gctctcaagg ggccgttgca cggtggcgcg
     5221 cctgggcctg cgctggatgt ggtgttcgag atcggggatg cgaacagggc cgaggcttac
     5281 ctgcgggaga agctcgggcg cggcgagcgg ctaatgggtt tcgggcacag gatctaccgt
     5341 gtacgcgacc cgagggcgga cgtgctcgcc gccgccgccg agcgcctgta caccacagac
     5401 ggggataggg agctctacga gctggcgctc gacgtggagc agacagcact cacgctactc
     5461 gcggagcaca agcctggcag gaacctgcag accaacgtcg agttctacac ggcgctactg
     5521 cttcacgggc ttcggttacc aacacaaatc ttcaccccga ccttcgcagt ggggagggtg
     5581 gcggggtgga cggcgcattg cttcgagcag agggcgctcg accgtctcat caggccgcag
     5641 tccgtgtacg tggggagtag ggatagaagc tgggtagcgc tcgaagaacg gtagtgtggg
     5701 gagacacgaa aacgaccccc ggagagaatc cgggggccgt acccttctga gcgttacgca
     5761 ggtatcccgt ctttcagcac gtccgccttc acaacatggg aggcggataa agtaacccag
     5821 gatcccgccg ggcgttttgg tgggcctagg tcagagccac ctctcccaga agatacccgc
     5881 gactactact cgatgcgtct atctggatca cctcctttcg cgtgccttca actccgcgaa
     5941 gtttactacc gcgctatgta tccagcaagg gtgatcctcg agattggttc tgttccaggc
     6001 ggagcctgag gactttctgg ccttttggac ccaattacta cggagaaggt ttacatctat
     6061 atctatgtgc ggtatgaata aaggtttacc agtgcccgta tcctcctttc cctttcccca
     6121 cctattaccc cgatgcgggc actggctctg agtactcaaa ccgtacctat attggccccc
     6181 tcgcgcaacc cctgtttaaa caggcttcgc gacgtttaac accgcttcat accctctctg
     6241 ccactaatcc tctcccacgc acctccatgt aggccgcttc gaccatcgca agtgatgtac
     6301 tcagacgtga gacaagctca cggtaatcag catctaacat gccctgcgag cgcaacaggt
     6361 taagcgtagg gtacgccgaa tccttccgga attcgtatac gagagcagga gagggcagat
     6421 gggtagaaaa tagcgtattt gcaggtaaat caagattggt tctcttccag gcggagcctg
     6481 aggaggtcga aggcctggtt cgcgcttcgt tcacggatcg agtctctgga gcgtgcccag
     6541 gcggtgaggt cgagtttctt ggcaaacgtg tcctctgaat cagagattcc gacgaagacg
     6601 agcccgacgg gcttctcctc cgtcccaccg tcaggacctg ctattccggt gaccgagagc
     6661 ccgtagtcgg cgccggagat cctgcgcgcc ccttcggcca tctcgcgggc tacgggctcc
     6721 gagacggcgc catgctccat gatcagggcg tgcggcaccc caagcaggcg ctctttagac
     6781 tcattcgagt aggtaacgag cccctctccg aagaatgcag aggatccagg catgtcggtc
     6841 aggcgcttgg cgaggagccc ccccgtgcag ctctctgcga gggccagggt ggcgccccgc
     6901 tcctggagca ggcgtccgac agcgccttcg agggtctcgt tgtcctcgcc gaagtagtaa
     6961 ccactgaggc gcgcgatcaa ctccttttcg accggcgcta tcttttcctg cgcctccttc
     7021 tcggtggcgg ccctggtggt gatacgcaag cgcaccttgc cctggcccgc cagtggggcg
     7081 accgtggggt ctgttgcgtc gaggaactcc tggaccttct ccgcgagcgc ggactccccg
     7141 atgccggcga agtggagggt cttggagacg atggaacctt cgctgcgggc ccggatcagg
     7201 ggctctagcg tcgcctcgaa catgctcgtc atctcggagg gaacacctgg gagcgtggcg
     7261 aacagcgtcc cttcccactc tacgagcgct cccatcgccg tccccaccgg gttggggatg
     7321 agctcggttc cctgggggaa aagggcctgc ttgtagttgt tggccgttgg cttgcggccg
     7381 aacttcgtga acatcgtgtc cacgtgttcc ctggcctcgg ggtactcgac catctctcgg
     7441 cccgtgagcg tcgagaggca agcgttggtc aggtcgtccg aagtagggcc gaggccgcct
     7501 gtggtgacca caaggtcgga gctggaggcg gcctcgcgca gggcggcgac gatcctctcc
     7561 gaattatccc ccacggtggt gtgacggtag atacccaccc cgagcccggc gagtcgctgg
     7621 ctgatccagg ccgtgttcgt atcgacgagg tcgccgagta gcatctcggt ccccacggtc
     7681 acgatctcgg ctctctctat gtttttccca tcaggcacgc aggtattcta caatccgccg
     7741 gatggaggac cacgcgaaga cccacactct cctcgaaagg gccgaagcct tcgcccgttc
     7801 caggctctcg gagaggcgtt acgaacacac cctgagggtg gctgacaccg ccgagagtct
     7861 ggcgctggct cacgatctcg aggcggacag ggcacgctta tctgccctgc tgcacgacgc
     7921 agcccgcgag aaggagcccg aagaattcct gaagctggcc gatgaatgga atcttcaggt
     7981 gggtgatccg gaacggcaga gcccgaagct gctacacggc tccgtggccg cagagctggc
     8041 aaggcgcgag cttggcatcg atgacgagga ggttctggag gcggtcaggg cccacacgac
     8101 ggggagcccg gggatgggac cgctcgcgct cgttctctac gtcgcggaca agatcgaacc
     8161 agcccgcgat tacccgagcg tgggacgcct gcgcactctg gccagggagg atttgcgccg
     8221 ggctgccgag gagtcattgc ggcgtgccat agcccacaac gaagagcgag gtaaggccac
     8281 gcaccctgcc agcctgagga tgctagattg gctggaggcc gatcgcaccg cgcaatccag
     8341 gatagagaag tagcagggcg taaagggtga aggcgagagc cacgagagcc acggcgacct
     8401 ccacagctcc tcccgacctg acacccccgg gcaaaatcct gaacggcttc ccaatgggcc
     8461 aaaggagcgg cacgccgcgc gtgttgagtg cgtcggcgac gacgtgcgaa gcgtagccgg
     8521 cgacgagggc cagagcaagg ttcggaaaac gtcccaggaa gagtaagacc agcagcgaaa
     8581 agaggagaac accgaacact gaatgggtca tggtgcgatg gccgacggtc ctgacgagca
     8641 cgaaagacgc cgtcctcagg gcccagctca atggccggct caggaggttg agcgtaggat
     8701 ttttcagccg gttcaggccg ttgcccaggg tgctccgcgg gttgtcgatg tcgggtagcc
     8761 aggcggcggc gacggcggca gggtagacgt aggcgggcgg gtcggccccc gtgacgagcg
     8821 cggcgccggc cagggctccg atcccgaaga cggcgtgggt ggtggcgttc aatgcggacg
     8881 cagtttagca acgaacctca cgcctaggac cctgacgaac cgaagccgcc ttcaccgcgg
     8941 ccgtcggtcg ccgactcgag gaactctacc tcggcgaaga acgctgaggc gacgcgcacg
     9001 aggacgagct gcgcgatcct cgttccctcc tcgatcagga aggtctcctg gcggtcgtgg
     9061 tttatcaggg ggacctggat ctcaccgcgg tagcccgagt ctatgaggcc aggcgtgttg
     9121 acgagggaga tcccatgcct cgcagcgagg ccgctgcgag ggagcacgag cccagcgtaa
     9181 ccctcaggga tggcgaccga gatcccggtc cgcaccaggg cccgggatcc aggcgccagg
     9241 tccaccctct cgaccgaacg caggtcatag cctgcatccc ccaggtgggc gcgctcgggc
     9301 atccccgcct cggggacgag acggcggaaa gggacctgaa gcgggcgttc ggagctcatc
     9361 cggagaatgg tagcaccgga aggtttcagg ctacagcagc tccagcgata cgccaccgcg
     9421 caagattcac atcagggcaa tgcccctgcc ttacggttga tacctgatgc ctttcgtctt
     9481 ctgctgctat ccttagttca tgagcgagca gagacgacgg tatctcgtaa cctcggcgtt
     9541 gccttacgca aacgggcctt tgcatgtagg gcagatctcg ggcgcttacc tgcccgctga
     9601 cgcgttcgtg cgttatctgc gcgcgcgtgg cgaggacgta gtctacgcct gcggcacaga
     9661 cgagcacgga gtccctatca ccctgacggc ggagcgcgag gggaaggacc cacaggaggt
     9721 cgtcgactac tggtgggtca acatgaagga gactttccaa cgtttcagca tctctttcga
     9781 taacttctcg cgcacctcga ccccgcttca cgcgaagaat acccagcttt tctaccgcaa
     9841 gctaaaggag ggcggctaca tctcggaggc tgagtccaag cagatgtaca gccctactga
     9901 gggccgcttt ctgccagacc gttacgtaga ggggacctgt cccgtgtgcg gctacaagga
     9961 ggcgaggggc gaccagtgcg acaactgcgg ctcgtggtac gaggcgtacg agctcatcga
    10021 gcctcactcc aaggtaacgg gcggacccgt cgaggtgagg gagacgacgc acctctacct
    10081 cgacctcccc gagttctccg acaggctgag ggagtatgtc gctggacagg agcactggcg
    10141 cacgtccgtc aagaacttca tcctcggcat catagacggc ggcctggaga agcggccaat
    10201 tacgcgggac atacagtggg gcgtccctgt ccccgagcct ggcttcgagg acaagcgctt
    10261 ctacgtgtgg ttcgacgcct gtatcggcta cgtttcttcg accatggagt gggcccagaa
    10321 catcggggaa cccgacaggt ggagggagta ctggcaggag cctgagagcc ggctcatcca
    10381 ctttatcggg aaggacaaca cggtcttcca caccctgatc tggcccgccc agctcatggg
    10441 caccgcagaa gacggtgagg aacccttcgt cctcccctac gacgtgccag ccaacgagtt
    10501 catgaacctg gaggtcctgg tcgacgggga acgacgggcg atgcagatgt ccacgagccg
    10561 taatctggct gtctggctgc acgaggctct cgaccgcttc cccgccgacc ctctgcgttt
    10621 ctacctgtcc tcgaacctgc ccgagacggg ggacgtcgtt ttctcctggc gggatttcca
    10681 gagcagggtg aactcggatc tgatcgggaa cctcggcaac tacatcaacc gtgtcctctc
    10741 ttttgcagag agatacttcg atggcgaggt tccacgcccc gaggagctac ctgaagatgc
    10801 cgtggcggtc ctggaagatt tcagggagtt agaggcgcgc tacgaggcaa aaatgctcgc
    10861 cccgaggccc cgcgaagcgc tcggagagct tctggcgatg ggcaggagcg ccaaccggtt
    10921 tttcgacgcg tcggccccct ggaagacccg caaggaggac ccggagcaga cccgcctcac
    10981 cctctatacc tgctccgtgc tgctcggctc tctcgcctac cacgcagcac cttacgtccc
    11041 tgaggctatt gggcggctcg aggagttctt cgacggccct atagcccgcg tctcggacct
    11101 cgatgatttg ccgaagggct accgcgccgc gggggcgaag cctctgttcc gcaggatcga
    11161 ggatgacgag gtcgcggcgg ctgaggggca actctccagg gccgtccgcg gcgaggcgag
    11221 ttaggcggcg tccgggctgg atagctcccc tttttcgggc ctatgccttg cggttcttgg
    11281 tcgatacagt atacgtgccg tcggggagtt ccccgagctc atagtcgccg aagaggatgg
    11341 gatcagcggt ccgcaggcac aggaagatgc ggagggccag gttgcggatc tcgctttcgg
    11401 cgtgcgcgtc ggcccgcatc tcgatgatgt gccttagggc gcgcacgttc cccgtgaaga
    11461 ccatcggggc ctccgtctcg ttcggcaaca gggagcgtgc ggtctgctgc accttctttc
    11521 tcgcgtcggt cctgtaatct gcggtgagca tgctcgcccc gccctcctgg cgctccagca
    11581 agagttcggc catcgcctcg tactcggtag cggccctgtc agcacgttcc tcgaaagctc
    11641 tgtgcaactc ttcgtcttcc tggtactccg gacgctccac aaagcgaagc acggctccgc
    11701 taacatagcg ttgtgaaatc tgggaaaaag cggtgccggc cctgtgtcgg accagctcgt
    11761 gggtgacgct gcggctgatg ccgtagagca ggaagttgaa ggtggagtgc tcgagcacgc
    11821 tgccgtgccc cgcagaggtc aaacgctcga tataagcggc ggcgttatcg ttcttcgtgc
    11881 gcctgggtcc gaatgacatg tagcacgtct gcccagccgt cttgcagagt tgggatgaat
    11941 ccggaagcct ggtggggtcg tcgaggtact cggtgaagtg gaggtccggg tcgaagcctt
    12001 cgaggaacac tccgagtcca gcaacgttgg tctggggctt cgcgagaacg acgactcccg
    12061 gtctcttgag ataaggggtc cctgcttttg tatggtgaac ttgcgaatgg atattgggga
    12121 agccgtcttt gacggctccg cccaactctt cgaacagttg ccgcgcccgc tcggtgttac
    12181 gctccgtggt ctccatgggc tcccttccca ttgttgcctc tggagagcac cttagcccat
    12241 ttgggcaggt tctacacggc ctcagagagg gcgcgacgca gggtgctggc gtagcggccg
    12301 gtgcggatag tccttagagc cttgatctcg acctgcctgg ctctctcctg ggagatgcca
    12361 agttcctcgg agacctcgcg caaggttagg gtctcgctgc cgtcgaggcc gtggcgcatc
    12421 tcgatgatcc tggcctcccg ctcgggcaga gacctcacag cctcgacgag cacccgctcc
    12481 cactggccga cctcgacgcg cgcgtagtcg tcgccggagc gctcgtcagg gagcaactcg
    12541 ccaaactcgg agccttcttc ggcgttgatc ttcgcattga tgctggagat gggctggctg
    12601 acctcgcgca gacgcctggc ctcctccggt tttacgccga gcctctcgac gagctcctcc
    12661 tcggtcgcgt cgcggccgag ttcgatgctg agagcgttct cggcgcgtcg caggcggtag
    12721 agcgcatcaa cgacatgtgc aggcagacgc acggtgcggg cgtggtcggc gacggcgcgg
    12781 gtgacggcct gacggatcca ccacgtcgcg taagtggaga agcggttgcc catctcgggg
    12841 tcgaacttct cgacggcccg tatcaggcct gcgttcccct cctggatcaa gtcctcgaag
    12901 agcacacctc gccccctgta cttcttggcg atggagatca caagccgcag gttcgactcg
    12961 atgagcttct gacgggctct catgtccccc ccgcggacac gccttgctag agccttctcc
    13021 tcatcggcgt cgagcagcct gctacccctc accctgccca agtactgtgt cagaacatcc
    13081 tgtgcttcca tcattacgtc tcccctctct tggctagtcc gcatttcagc tttcgctcgc
    13141 cctcgctgac aggctgacta tctgaggtgc tcttcatcat gccgcggggt acgatccccc
    13201 gacccgtggg cctccgtggg ttacgaagcg ttccaggatg cgggggtcga ggctggatgt
    13261 gatgtgccac ctccgcccgc acgagcaatc cagctcgagg agccgccgct cgcccacttc
    13321 ggacatggcc gtctctttga gggatcgcag gcagcacgta ggcaacgacc acacgtagat
    13381 ctcctgctcg ggttcgccta cggggacgag cgcggtatcg ggcctgaggg cgctccgcct
    13441 cttgcgcgct tttcgctttg cctctccgct caccttcgat ctccctctct cccccatcgt
    13501 ttgccaacct tcgtccctac gctgctcctc tacgctcttc gcgctgtgcg aaggcgtcct
    13561 cctcgggttg cactggcagc ggctccctcc acatcgcgcc gcatacaggg cactcgaacg
    13621 agtcgtcgta gtgaagggcg ctgcgcctgc agcaggcagg gaggtcttcc tcgtagacga
    13681 cttcctcgcc gagggcagcg atccaggcgg acccacccac tacgcggccc tccctgagag
    13741 acgcccgatc cactcgctcg cctcctcgcg ggtgagctcc cctacgggct ttccgaccat
    13801 ctcctcgaac cggccgatcc catcttcgac gacgtccgag attagggcct ccaggtaatt
    13861 gagctgcttc ctggtcgccg gaagctcgcc tcttccttcc tcgttctgcg ccctgggccg
    13921 cccttcacgc tcctgcgtcc ccgaagcctc aacgacctct tcctcgccgc cgagctcctc
    13981 gaaagcggtt acacctacgt tgatggcgtc cctgagagca cgcgccttgg ccctcgtctc
    14041 agccatccgg atcaggtgcg gagctatcgt ccggttcacg ttctgcgggg aagcgtcacc
    14101 gatgccggcg aacttgccct cctcagtccg gatcacggcc ttcacgatcg ccacctcgcc
    14161 gttctccttt gcaggaactt gcaagagctc cgtctcgatg ctcctgagcc ccctgctgtg
    14221 cgcctcctca aggagccccg catagagcac gaacctcttg ccctggcgct cgatcatgta
    14281 ctcttccctc atcacttaac ctcctccgac cagaagccgg taacgctatc ttcactgaac
    14341 ttggtgagga tgtggtcaaa aataaacatc aggcgatccg ctcgtaggta tactggcgtc
    14401 cgccgggggc cgcggtctcg atgcggcggt ggatcagtcc gagtttcgcg agtctggtcg
    14461 ccctgttgtt gcaggctgta tggttgagat ccagcctcgc agcgagggcc gcgttggtga
    14521 tggagcctgc gtcgtagatc gcctgcaggg tctcgaccag atggtccggc accttgccga
    14581 ctatgccagg ctcggccccg atctcatcga cccggatcat ggccagcgaa cgctgcctca
    14641 gagaggcctc gacgttctcc aggagatccc ggtcctcctc gaccaaaacc agatgcttgt
    14701 ccccatactc cccgctcgca gatctcgacg ccagtatccc aagcgcctcg tccgcgaacg
    14761 agtagtccat gagctcgacc ccacgcgtgt ccacatacag gatgccaccc ttcggcaatc
    14821 cctccagcgc tccttcgagc gcctcacgaa ccgcacggcc cgtcctacgg gtcaccatca
    14881 acttgccacc gaagtcgccc ccagcaacct cgaagacctg gtgcgcccgc tcctcaatca
    14941 ccggagctgt cctcactgtc gtcagtgaaa tcatatatga gcattgtacc ggtcttccct
    15001 ggataagtca acggcgtatt gggggtattt tgggttcatc ctgatgtttt ttcctgtggt
    15061 gtacgtggca gggagacgcg gacgaaggtg cctgggaagt cggggacgtt ccgggagttc
    15121 ttacggttct cgtagacggt tacgcggcct gatcctgagc gcaaagagag cgagccgccg
    15181 gcgcgggcgg cgatccccgc gaccagggcg agtccgcctc cgcggccgat ctcaccggtc
    15241 gccgagacgc ccatctcgag tgcgtggcgc agggcgtcgt tgtcggtggt ggtatgttcg
    15301 gcgtactcgg ggttacgagt gagcgtctcc cgcacgccga cgcctccgtc ggcgatcgcg
    15361 atgaggacct cttcgccgcg gcggcgggac ccgctaacga tgtggtggta ggcctggacg
    15421 gcggcgtagg ccccgaaaga ggagacgccg tgctcggcgg cgttcgcgca gacctccgag
    15481 agcgtcgagc agatcgcgac ccgctcgcgc aggcggtagc cctggttctc caggatctcc
    15541 gagattctct cgatgatccc tggtatctcc tcctcggtgt ggaagttgat caactcctgc
    15601 agcgtccccg ggttgtgccg cctgcgctcc tcgagagagg ccacatcggc attggtgcgt
    15661 accgtttcgc cgaacgacct gaagaagttc atccgctcca gataggccgg cacgtcgcgt
    15721 cctgcgaggg caagatccac cacctcgcag cgcgcactca ggtactccag taatgctgcc
    15781 agggcgcaga gtcctgcagg ctcgacgaac tcgaccgtgc gcagatccac gaccacaggc
    15841 tctccttgca cgcttgtggc ggcggcgaat gcgggatcga ggctgcccat ggtaagcacc
    15901 ccgctcatcg cgccccggct cctgatgttc agttccagct gtgtcttcat cagccttatt
    15961 gtagtcggtt aggcaaggaa gcggacggga agctgtgcag gggcattaca tgacacggca
    16021 tcttgcttct ggagaagggc gtcctcacag cggcatgcag aaggtgccgg caccttctgc
    16081 atgcctggca tcaggcaaca ggtttcagga tttgcaaggg atggtttttt tgctgaggac
    16141 accttctctc cagtggcatg gctcacatct tacgaaaagc gctgttacgg tttgtgatcc
    16201 tcccgccggc gttcgtagaa gagctcggtg tagcggcggt tatcgtagcg gctcaggagg
    16261 gaaccgtcac cgacccgcac aaagccgttc ttctcgaaga ccctctgcat gcggtcgttg
    16321 tgggccaggg tcgagccgcg cacggctgcg aggcccaggg tgtggaagac gtagtctatg
    16381 aggagggtaa cggcttccgt agcgtagccc ctaccgcgaa ggtttccggg tcctatcttt
    16441 atgcctagtt cgcagccgtc gcggcggaat tcataaaggg tcgccatgcc gatcggctcc
    16501 ccacgggcct ctatgataaa cgttcttacg ctcgggtcct cccagcgggc caggtagtcg
    16561 cgccgggccg ccgagaggga gatggagatg ggtgagcgcc cattccaggc ggcgagctcc
    16621 gggtccctgt cccaggagta gactacctcc acgtcggatt cccgcggcgg cctgaggatc
    16681 accgattctc cctgtagagc ctttacctct tctatacgta tggtgtctcc tctctcaaga
    16741 aacagaatgt tacacccgtc gccagcccga ggcatccttt cgggtgtgta cattttagtt
    16801 agaatagccc aaagcaggag atcgggagcg tcattgagtg gtacggttgg ggagctcttc
    16861 gggggttcca gcagaagagt cagggacgtg atctgggggg acgtctctat agatcgggcg
    16921 gtgaagacgc tgctcgagac tggcgcgatg caacgtctga ggggcatgag ccagctcgga
    16981 tttaccttcg gatccttccc gagggcccgt cacacgcgcc tggatcacgc ggttggcgtc
    17041 taccacctcg ctgggctgac gctaaagcgg atcgcggagt ctggcgccta tctcgaagac
    17101 cgcgacgtga gggccgccct ggccgctgcc ctgctgctag acgccggccg atatccttac
    17161 gctgcttctg tcgaagggat caaagtccca ggcgcgccca ccctcaagga gttgagcagg
    17221 cgttggatcg aacacaccga agtggcgaag gtcttgaggg cagagtggga cctcgagccc
    17281 cacaacgtct tcagactggt ctcgcgcggg gataccccgc cacacagcct gacccctacg
    17341 gaacacctgc taagggacat cctcttcggc tcgttgaacg tggatgccct cgaccgcctg
    17401 atacgcgacg ccaggggagc cagggtaccc tacggccttg tcgaggtaga gtccctcata
    17461 cagcgcctga ggatcgtggg ccaggagaac aaggcgatac tggcgctgga cgaagaaggg
    17521 gcgggtacac tgcaatccgt ggtcttctcc cgctacctgg tgtactacaa cgtctacggg
    17581 caccacgctc tgcgtatccc gaccgtaatg ttcctcaggg ccgtccagga cgccataggg
    17641 gctggtaacg tgaccttcga cgagctctcc gagctggatg atgcgggagc cttcgtcctg
    17701 ttggaggatt ctgccgagag ctccacagcc gccctcatga aacgccttgc agaccgcagg
    17761 ccctaccacc tcgccctgga gttcgatgag cgacacccga gctactcctc actgatccgg
    17821 ctccgcgacg acgcctcctg gcgccgccgg gtggaagagg cctggtcccg ctatctcacc
    17881 cgctaccgca aaggtgtggc cggaccattc gacatcctca tagacctccc cgcaagagag
    17941 cacttccccg tggagttgcg cctgatccga cacaaccccc tccccggcga acgcaacccc
    18001 ataggctggc ataatctctc tggcatgtcc gaagacgaaa tgacgcgcta ccatgccccc
    18061 ctccgccgga tactgatcgc cacagcgaac gacgacctcg tgaagtccgt gcgccggcac
    18121 gccgaagaac tcttcaccat cgccgaggaa gtaggataat cagcacttca gcatttcagc
    18181 acgcgccctg tcgggcgctt gtcagcacgt cgcctgtcac aggcgacttg cagcatttca
    18241 gctaggcagc tttcgcatgc cgacgtcctt gctgagtggc tgacaagatg gcgcagccgc
    18301 cgtgctgact tgctgacaag cgaaggccta aaggccggag cgtgctgaaa tgctgaagtg
    18361 ctgaaatgct caaaacaaaa gggccggtgt tcatagaagg gtgagaccct tttccggccc
    18421 ttagcggccc ccagataggg ccgctaggat gatacgaaga ttataaatga gcggacgcct
    18481 ccgtcgcaag ccctgtgaac aaaacttaac tttggcccta tactccccgc gagcggtgtt
    18541 ataattcgcc ctcgcgggcc gctagctcaa ttggcagagc aagagactct taatctctag
    18601 gttccaggtt cgagtcctgg gcggctcact cgaaaaaccc tctgtttgca ggtaaaacgc
    18661 gacggcgcta ggaagccccc gatg
//
LOCUS       DASTKM010000102         5351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_551973, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000102 DASTKM010000000
VERSION     DASTKM010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551973"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFQ10_04200"
     CDS             <1..534
                     /locus_tag="VFQ10_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET9964574.1"
                     /translation="FSREGVTLFEWGFLEVHSGGLRLGFFLALRILLLVSSAGLLTAT
                     TAPVALADGIEDLLSPLKRVRFPAHEVAMMMTIALRFIPTLGEEAEKIKRAQAARGAD
                     FSEGGPLRRARSLVPVLVPLTVGAFRRADELADAMESRGYRGGEGRTRYREMRFRVSD
                     ALALAVTLLILLVGILL"
     gene            531..1292
                     /gene="truA"
                     /locus_tag="VFQ10_04205"
     CDS             531..1292
                     /gene="truA"
                     /locus_tag="VFQ10_04205"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET9964575.1"
                     /translation="MKLAGLVEYEGTGFAGWAAQPERRTVEGVLSDALSTVLRQPVKM
                     SAAGRTDAGVHASGQVVSFRAQTELGPALISYKTTAVLPKDVALRRCVAVREDFDARR
                     DARSRTYEYRLVNDEIRSPLQRHHAGYAPQRLDLELLQEAARLVEGTHDFRAFTPQKS
                     YHVRFERIVTRSRWSRHDDLLRYRISADSFLYGMVRTLVGTMLEVAGGKRDLTEFDRL
                     LSGGKRSEAGPAVTSRGLTLVGVGYDDLDLWRSER"
     gene            1289..1957
                     /locus_tag="VFQ10_04210"
     CDS             1289..1957
                     /locus_tag="VFQ10_04210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET9964576.1"
                     /translation="MNTDDTRELLREIDAYVEDLFAPPDEILEAALRDSRRAGLPGIN
                     VSPNEGRLLQLLTEIAGSRRILEIGTLGGYSAIHFARALPDDGALISLEIDEHHAGVA
                     RNNVGRAGLSEKVEIRVGDAHELLAAIIEDEEGPFDVIFIDADKESYPEYLDASLRLV
                     RPGSLILGDNTIRGGTVLDPKDSTAQATREFNERIARDPSLCSIALPLIRERIDGLTI
                     ARVL"
     gene            2132..2566
                     /gene="rplM"
                     /locus_tag="VFQ10_04215"
     CDS             2132..2566
                     /gene="rplM"
                     /locus_tag="VFQ10_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET9964577.1"
                     /translation="MMKSYMARPLEVERKWYVVDAEGKHLGRLATEIVRVLRGKNKPQ
                     YTPHVDVGDFVVVVNADRIAVTGRKAQQRVYRRHSGYPGGMKETSYEQMLARKPTEVL
                     RKAVYGMMPKTRLARKQFKKLKIYAGPEHPHSAQDPQTLEVK"
     gene            2567..2959
                     /gene="rpsI"
                     /locus_tag="VFQ10_04220"
     CDS             2567..2959
                     /gene="rpsI"
                     /locus_tag="VFQ10_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET9964578.1"
                     /translation="MAEALYSGTGRRKTAVARVRILPGDGKIIVNGRDVDEYFPRPAY
                     RTAVRLPLELLGSAGRYEVVAKIEGGGPTGQAEALRHGIARALAEESQEARSELKAAG
                     MLTRDSRAVERKKYGLKKARKRPQFSKR"
     gene            2979..3167
                     /locus_tag="VFQ10_04225"
     CDS             2979..3167
                     /locus_tag="VFQ10_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964579.1"
                     /translation="MSGSFRAAGGARSTSIPGGGTGTSPDEHIEYAAFEAEAFCDPFL
                     HRRRQGFRIYPSMVKEAD"
     gene            3164..4492
                     /gene="glmM"
                     /locus_tag="VFQ10_04230"
     CDS             3164..4492
                     /gene="glmM"
                     /locus_tag="VFQ10_04230"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET9964580.1"
                     /translation="MRNRTITFGTDGVRGVANKGLLPEDALRLGLAASRVFGGKILVG
                     RDTRLSGEMLASALCAGAASGGADVVDLGVLPTPGVAALAPRLGATAAGVVSASHNPY
                     PDNGIKFFSGEGRKLSPEKEREIEDLLGRDITRPTGSGVGGVEVLADATRMYAAWVLR
                     KLRPSAEGVKVLLDCANGAAYEVAPLVFREIGVDLTLTGADPTGTNINEDCGSTHIEE
                     LDASGHDVAFAFDGDADRVLALDEQGNVVDGDRIIAILARDKKKRGVLSGEVVVTVMS
                     NLGFFKAMEAMEIAYDVTDVGDRHVAEAMDRLGASLGGEQSGHVILSEHTTTGDGLVT
                     ALALLDVMVRSGRPLSELAAVMKVYPQTLINVRVEEPGAAKAVAASETVRLAVSEARS
                     TLGEEGRVLLRPSGTEPVVRVMVEHSDETVCREVCEGISRAVYDAGKEGT"
     gene            4489..5346
                     /locus_tag="VFQ10_04235"
     CDS             4489..5346
                     /locus_tag="VFQ10_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964581.1"
                     /translation="MRFVGCALAWRPGEARDKVSCLVVLDERGSIIANSFVADASEIA
                     ETVEDYGSERRGIVVGVDAPLAVPNERGTRRIERILSKVALPAYSASRRMFGGEPYAE
                     ELLSELEKVGVEYTDYPFPRERGQSVVVEVDSAATLKVLSLERSGAADNGDLTKRLRA
                     MDDPKLRKGNKESRAAALKGAIEMLWDTPGLRLRTGNLSGDLSASQNVDVSKLDISAS
                     MSHADLDRTVGLVEGTLAAYTVHRHWRGRDGSIVVGAGDEGSVLLPARNALRKRLAEE
                     CGNAGVPYV"
BASE COUNT         1011 a         1633 c         1838 g          869 t
ORIGIN      
        1 ttctcgcggg agggggtcac gctcttcgag tggggcttcc tggaggttca ttccggcggg
       61 ttgaggttgg gtttcttcct ggctttgcgg atactattgc tcgtctcctc cgcagggttg
      121 ctcacggcga cgacggcccc ggtggcgctc gctgatggga tcgaagacct cctttctccc
      181 ctgaagaggg tgcggttccc tgcgcacgag gtcgcgatga tgatgaccat agcgctccgt
      241 ttcatcccca cgctcgggga ggaggcggag aagatcaaaa gggcccaggc agcccgcggc
      301 gctgacttct ctgagggagg gccgcttcgc agggcccgct ctttagtccc cgtactcgtg
      361 ccgctgaccg tcggcgcctt ccgccgcgcc gacgagctgg cagacgcgat ggagagcagg
      421 ggctacaggg gcggcgaagg ccgcacccgc taccgggaga tgcgcttccg ggtcagcgac
      481 gcgctcgccc tggccgtcac cctgctcatc ctgctggtgg ggatcctgct ttgaaactcg
      541 ccgggctcgt cgagtacgaa ggcaccggct tcgcgggatg ggcggcgcaa cccgaacgaa
      601 ggacggtcga gggtgtactc tccgatgcgc tctctaccgt tctgcgccag ccggtcaaga
      661 tgagcgccgc gggccgcacc gatgcagggg tgcatgccag cgggcaggtc gtctccttca
      721 gagcccagac cgagctcggg cctgccctta tctcctacaa gaccacggcg gtactgccca
      781 aagatgtcgc actccgtcgt tgcgtcgccg taagggagga tttcgacgcc cgcagggacg
      841 caaggagccg gacatacgag tacaggctgg tcaacgacga gatcaggtcc cccttacagc
      901 gccaccacgc gggctacgcg ccgcagagac tcgacctgga gctcctgcaa gaagcggcaa
      961 gactggtgga aggaacacat gacttcaggg cctttacccc tcagaagagc taccacgtcc
     1021 gcttcgagcg tatcgtgacc cgctcgcgct ggagcaggca cgacgacctg ctaaggtacc
     1081 gcataagcgc cgactcgttc ctgtacggta tggtccgcac cctggtcggc acgatgctgg
     1141 aggttgcggg cgggaagaga gacctgacgg agttcgaccg gctgctctcg ggaggaaagc
     1201 gcagcgaggc aggacccgcg gtaaccagcc gcggacttac gctggtcggt gtgggctacg
     1261 atgacctgga tctatggagg agtgagcgat gaacacagac gatacgagag agctcttgcg
     1321 ggagatcgac gcctacgtcg aggacctgtt cgccccaccg gacgagatcc tcgaagcggc
     1381 tttgcgggac tcgcggcggg ccggactgcc tgggataaac gtctcgccca acgaaggaag
     1441 gctgctgcag ctgctcaccg agattgccgg gtcgcggcgc atactggaga taggcaccct
     1501 gggaggctac agtgccatcc acttcgcccg cgccctgccc gacgacggag ccctgatctc
     1561 cctggagata gacgagcatc atgccggagt cgcccgcaac aatgtcggac gggccggcct
     1621 ctccgaaaag gtcgagatac gggtcggtga cgcccacgaa ctcctcgccg ccatcatcga
     1681 agacgaagaa ggtcccttcg acgtcatctt catagacgcc gacaaagaga gctaccccga
     1741 gtacctggac gcgtccctgc gcctcgtacg accaggctct ctcatcctcg gcgacaacac
     1801 tataaggggc ggcaccgtac tcgaccccaa ggactcaacg gcccaggcga cgcgcgagtt
     1861 caacgagcgt atagccagag accccagcct ctgcagcatc gcgctacctc tgatacggga
     1921 gcgcatagac ggcctgacca tagcaagggt cctgtagaac ggaagataca tgtgacgatg
     1981 agcatacgcg cgatcagagc acggtccgtg tgggggcggt tcgcgcaccg ccgaacgcct
     2041 gtgggccatt ctttgaggct ggtccttttg ccgatgtatg ctactatttg atccgttcga
     2101 aggcctttga agttcgcaca agctccggag gatgatgaag agttacatgg cgcggcccct
     2161 ggaggtcgag cgaaagtggt atgtcgtcga cgccgaggga aagcacctcg gacgtctggc
     2221 gacggagatc gtacgcgttt tgcgcggcaa gaacaagccg cagtacacgc ctcacgtcga
     2281 cgttggcgac ttcgttgtcg tggtgaacgc ggacaggatt gccgtcacgg gcaggaaagc
     2341 ccagcagagg gtctacaggc ggcactcggg ttatcccggc ggcatgaaag agaccagcta
     2401 cgagcagatg ctcgcgcgca agccgaccga ggtcctgcgc aaggccgtct acgggatgat
     2461 gccgaagacc cgcctcgcca gaaagcagtt caagaagttg aagatctacg cgggacccga
     2521 gcacccacac tcagctcagg acccgcagac gctagaggtg aagtagatgg cagaggctct
     2581 ctatagcggg acgggaaggc gcaagaccgc cgttgcacgg gtaaggatac tccctggcga
     2641 cggtaagatc atcgtgaacg gccgcgacgt ggacgagtat ttcccgcgtc cggcctaccg
     2701 cacggcggtg cgcttgccgc tcgagctcct cggttcggcc ggccgctacg aggtcgtagc
     2761 caagatcgaa ggtggcggtc ccaccgggca ggcagaggcc ctgcgccacg gtatagcacg
     2821 ggccctcgcc gaagaatcgc aagaggcccg tagcgaacta aaggcagccg ggatgctcac
     2881 ccgggattcc agggccgtcg agcgcaagaa gtacggcctc aagaaggccc gcaagcgccc
     2941 gcagttctcc aagcgctgag ccgcaagagc ggatcttcat gtcgggctcc tttcgcgcgg
     3001 cgggaggggc ccgttctacg tccataccgg gaggaggaac cggtacgagt ccggacgagc
     3061 acatcgagta cgctgccttc gaggccgaag cgttctgcga tcctttcttg cacaggcgcc
     3121 gccagggctt caggatctac ccgagcatgg taaaggaggc agattgagga accggaccat
     3181 aacgttcggc accgacgggg tgcgcggggt cgccaacaag ggcctgctgc ccgaagacgc
     3241 actgcgcctc ggcctcgccg cctcccgtgt ctttgggggc aagatcctcg tcggacgcga
     3301 cacgcgcctc tcaggagaga tgctcgccag cgccctgtgt gccggcgctg cgagcggtgg
     3361 cgccgacgtc gtagatctcg gtgtcctgcc tacgcccggc gtcgcggccc ttgcgcctcg
     3421 tctcggggcc acggccgcgg gtgtcgttag cgcctcgcac aatccctacc ctgacaacgg
     3481 catcaagttc ttctcgggag aaggccgcaa gctctcccct gagaaagagc gtgagatcga
     3541 agacctgctg ggccgagaca ttacgcgccc caccggaagc ggggtcggag gggtcgaggt
     3601 gctcgccgac gccacacgta tgtacgcggc gtgggtgctc cggaagttgc gtccgtcggc
     3661 tgagggtgtc aaggtgctcc tggactgcgc caacggggcc gcctacgagg ttgcgccgct
     3721 ggtgttccga gagatcggag tggatctcac cctgaccgga gccgatccca cgggtacgaa
     3781 cataaacgag gactgcggct cgacgcacat cgaggaactc gacgcctcag ggcacgatgt
     3841 ggctttcgcc ttcgacgggg acgcagacag ggtgctcgcc ctcgacgagc aggggaacgt
     3901 ggtcgacggg gacaggatca tcgccatcct cgcccgcgac aagaagaaac gcggagtcct
     3961 ctcaggcgag gtcgtggtca cggttatgag caacctcggc ttcttcaagg ccatggaggc
     4021 tatggagata gcctatgatg tgacggacgt gggtgacagg cacgtcgccg aggcaatgga
     4081 caggctcggt gcttcgctgg gcggggagca atcagggcat gtgatcctct ccgagcacac
     4141 cacgacagga gacggcctcg tgacggccct cgcgctcctc gacgtgatgg tccgcagcgg
     4201 cagaccgctc tccgagctgg ccgcggtgat gaaggtgtat ccgcagacgc tcatcaacgt
     4261 gagggtcgag gagcccgggg ccgcgaaggc cgtcgcggcc tcggagacgg tacggctggc
     4321 cgtctccgag gctcggagca ccctcggcga ggaaggacgc gtccttttga ggccgagcgg
     4381 gacggagccg gtcgtgcggg tgatggtcga gcattcggac gagacggtgt gccgggaggt
     4441 ctgcgagggg atctctcgtg ccgtgtacga cgcgggtaag gagggaactt gaggtttgta
     4501 ggctgcgcgc tggcgtggag gccgggcgag gcgcgggata aggtctcgtg cctcgtggtg
     4561 ctggacgagc gggggagcat catagccaac tcgttcgtgg ctgacgcgtc ggagatagca
     4621 gagactgtcg aggattacgg ctcggagcgg cggggtatcg tcgtcggcgt cgacgcgccg
     4681 ctcgcggtcc cgaacgagag ggggacgcga aggatcgaac gcatcctctc gaaggtggcg
     4741 ctccctgcct actcggcgag ccgcaggatg ttcggcggcg agccttacgc agaggagttg
     4801 ctctccgagc tggagaaggt tggcgtggag tacacggatt accctttccc gagggagagg
     4861 ggccagagcg tggtcgttga ggtagattct gcggcgacgc tgaaggtcct ctccctggag
     4921 cgctcgggcg cggcggacaa cggcgacctc acaaagcggc tccgggcgat ggatgacccc
     4981 aagctccgta aaggcaacaa ggagagcagg gcggcggcgc tcaagggcgc tatcgagatg
     5041 ctctgggaca cgccgggcct caggctaagg acgggcaacc tctccgggga cctctccgcg
     5101 tcccaaaacg tggacgtctc caagctcgac atctccgcct cgatgtccca cgcagacctc
     5161 gacaggacgg tcggcctggt tgaggggacg ctcgccgcct acacggtcca ccgtcactgg
     5221 aggggaaggg acggctcgat cgtagtgggc gcaggagatg agggctccgt gctgctgccg
     5281 gcgaggaacg ccctgcgcaa gcgcctggcc gaagaatgtg gcaacgccgg tgtgccctac
     5341 gtgtagcccc g
//
LOCUS       DASTKM010000103        13454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_23925, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000103 DASTKM010000000
VERSION     DASTKM010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13454
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23925"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /gene="mutL"
                     /locus_tag="VFQ10_04240"
     CDS             complement(<1..560)
                     /gene="mutL"
                     /locus_tag="VFQ10_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET9964582.1"
                     /translation="MRLFPRLLSEEAAGYAGLVRVLSPEVAHRIAAGEIIERPASAVK
                     ELIENSLDAGASRVEVEIEDGGVALIRVSDDGSGMSPEDAQRAVAEHATSKIRTADDL
                     ASVVSLGFRGEALHAIGAVSDLTLTTRERGNEDSLGARVRVFGGKIVESASAAHTQGT
                     TVEARDLFLNLPVRRGFLGTVRAEANA"
     gene            complement(567..2921)
                     /gene="mutS"
                     /locus_tag="VFQ10_04245"
     CDS             complement(567..2921)
                     /gene="mutS"
                     /locus_tag="VFQ10_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET9964583.1"
                     /translation="MLGRYAELKAQLPPETILLYQVGTFFETFEEDAKRISQALSIRL
                     TSREAAGEGRVPLAGVPGHALQEHLATLLRKGYSVAVAEQRQHPTKPKQFTREVTRIL
                     TPGTVIEDNVLSAGQANYLAAFVVRDGRAGIAVVEASTGEFTGTEVSEEQLPAELERW
                     SPREIVVPERTAAEKLPKVRAAVSSAPRWTFEPSAGEQALKRHFKVSGLKGYGLDGRP
                     ALAGAAGALIQYLASLRGGGPPEQVVTFRPYDPGSGMVLDAATRRNLGLEELVETVDR
                     TRTPMGRRALRRWLERPLLEVDRIRQRLGAVDVLTGDYMLREDVREHLGRIPDVERIA
                     TRIVRLSASPNDLLSLKGALEEIGPLRETLEPASQGSSLLSRVLGAMEEPPGVRELVA
                     AAISEESGEIIRPGYSGELDEAREFRDGAHEWLTRFEAEERLKSGLKTLKVGYRDGEG
                     YFIEVAGKETSAVPERYQHRKALKHNARYVTVELKEHESRMLGARDEVERIERSILAE
                     IRAAVKEAAPELQKVSRAIAVVDVISSFAAAATELRYCRPEVTQGRGIRIFGGRHPVV
                     EHNLEMPFVPNDSEVDGDSRLQIITGPNMAGKSVFLRQVALISLLAQTGSYVPADEAS
                     LGVIDRIFTRVGAEDRLASGESTFMVEMTEAASILNSATERSLVILDEVGRGTSTYDG
                     MSLAWAIAEYLHDDVQALTLFATHYHELTRLVEMLPGCRNYKASVEEVGGEIVFLHRI
                     ESGAESSSYGVHVAKLAGLPPQVTDRAGEILARLEAEGVKDFAG"
     gene            complement(2983..3996)
                     /locus_tag="VFQ10_04250"
     CDS             complement(2983..3996)
                     /locus_tag="VFQ10_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964584.1"
                     /translation="MFTAIVFVVALVAIILGAAFFTNAIEILGGRLGLRQGAVGSLLA
                     AVGTALPESMIAIVAILEPVLTGEASEEGALIGIGAILGAPFMLATLAMFVVGVSTLF
                     FRRRRDQGTRLRVDVATVGRDVGFFLVFFAMAAGVGLVELPLYIKVIVALVLAFGYGL
                     YVRRTLSSGEHLEEVPKRLLFLPRRQEPPHFAVVLQTLASLGVIIAGAHFFVDAVEHA
                     AAGLGLPAGLIALILAPLATELPEKFNSIFWIREGKDTLALGNVTGAMMFQSTVPVAF
                     GVLFTPWNLAPLDLFAVVLALASGGLVYLTLRVSGKLRAGRLMLGGLFYIAFVVGAVV
                     AVL"
     gene            4370..5293
                     /locus_tag="VFQ10_04255"
     CDS             4370..5293
                     /locus_tag="VFQ10_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET9964585.1"
                     /translation="MSPKPVILDVDPGHDDAVALMLACGHPDLDLLAVTTVAGNVPIE
                     KTTRNTLRVLSFVGCTHVPVGVGASEPLERPLHTAEDIHGKSGLDGAEEIPDAGFEPD
                     YRGAVALIADTLRSSTEPVTLIPVGPLTNIAAFLREHPDLRDSVAHISLMGGSMGLGN
                     TTPAAEFNIYVDPEAAREVFESRLPITMSGLDITHQAGAGPLERMRLRATGRVGGVVA
                     GFLDYFAATYESVFGFDAPPLHDPVAVAAVLELGLLKTRPMRVDIECESDLTRGETVC
                     DFYGVTGRAPNAEVGVDLDHQGFFDLLYGVL"
     gene            complement(5340..6521)
                     /locus_tag="VFQ10_04260"
     CDS             complement(5340..6521)
                     /locus_tag="VFQ10_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9964586.1"
                     /translation="MQGKDSAKRLSGRRLRRAFYLVLLAVLIGLLISYWSWVDAQVRG
                     VVVISSVLDAPVLTPAVEAAGGDPRFSQARVAGNPSLVVRPAGERPWPAIFLVNGAVR
                     EGRKLPEVRNLAEGFARAGYLVVVPDLPGLTEARITPQTADATTQVAREISGRPDVED
                     GKVALVGVSTGATLALLAAEDPALRGKVSLVAGVAPYSNIKTVLSLATTGHYRRPGGE
                     LVRYEADSFLSYVVARSLVAALPPGEDKLTLAAELERAGRESPDPLSGFSSRRTDDLG
                     PGAKSVVRLIANRNPERFAALYANLPDDVRHDLEELSPLAGTERIRVPVELVTGPRDK
                     YFPPSQSYALERVAPQRRVTVTGALDHAKLEVSPEDIQAFATFDAFVVRSLRTARAVE
                     S"
     gene            6689..7141
                     /locus_tag="VFQ10_04265"
     CDS             6689..7141
                     /locus_tag="VFQ10_04265"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9964587.1"
                     /translation="MADLPQQGESLPDLDLTTESGEHIGTRGLTGQKTVLYFYPKDDT
                     PGCTKEACAFRDRMDEYAKAGIQVYGVSLDSPESHREFREKYSLNFPLLTDEGGRASE
                     ALGVLREDRKRTKRVTFLLDPEARISKVYPEVTPDTHAEEILEDAASI"
     gene            complement(7279..8073)
                     /locus_tag="VFQ10_04270"
     CDS             complement(7279..8073)
                     /locus_tag="VFQ10_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9964588.1"
                     /translation="MAEVNGARIYYEVAGEGDPLVLVHAGIADSRMWEGQLTAFADRY
                     RVIRHDMRGFGMTAMVDGPFSHYEDLRGLLDSMDVERAHLVGCSMGGGAVLDFALEYP
                     QRVGDMVLVGSAVGGFGPDIDPPRQWDDIVAAEEAGDLERVSELEVQVWVDGPGRSPE
                     DVDASVRDLVREMNLIALRNEASGLGDEWEPEPPAADRLPDVQAPTLLIVGDEDQPSV
                     FAAADLLQRELPNVRKVVMHGAAHLPNMERPEEFNRIVLDFLKDYR"
     gene            complement(8249..8464)
                     /locus_tag="VFQ10_04275"
     CDS             complement(8249..8464)
                     /locus_tag="VFQ10_04275"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET9964589.1"
                     /translation="MLPSDKKTECPYKGVASYYSVQAGGETREAIVWYYPTPIPAAAG
                     IEGHLCFFNEKVDLEVDGEAQQQPQWS"
     gene            complement(8905..9177)
                     /locus_tag="VFQ10_04280"
     CDS             complement(8905..9177)
                     /locus_tag="VFQ10_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964590.1"
                     /translation="MAYRIVVASLVVCLLLVVGMLLLERTGFIEIDEKAIQPEVVSCQ
                     IGHKDMEVRVLGARAAYRGEVVPRYEFWLPPFGGCAVYYWDRPNRD"
     gene            9336..9698
                     /locus_tag="VFQ10_04285"
     CDS             9336..9698
                     /locus_tag="VFQ10_04285"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9964591.1"
                     /translation="MPESYLPLTPAVLDIMVALGNEELHGYAIMQEVRRRTDGQRHLA
                     PGTLYRSLKQMEQRGWVAQSEERPEPNLDDERRRYYRLTEIGRRVALAEVERLEGLVG
                     AARLKGFIPSPRPSGADA"
     gene            9806..10000
                     /locus_tag="VFQ10_04290"
     CDS             9806..10000
                     /locus_tag="VFQ10_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964592.1"
                     /translation="MEKELLLALGRCGKLTAVEAALESSLSVEEAERMLQELAAKGHL
                     EVSIEHGRLHYALWDREAPL"
     gene            10131..11078
                     /locus_tag="VFQ10_04295"
     CDS             10131..11078
                     /locus_tag="VFQ10_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964593.1"
                     /translation="MARRWTFYLPVMIGAAVAVACAVALSALSEKAEATFPGKNGRIA
                     YSAHDGNDYEIYTINSRGGAKRKVTNNNRDDYSPSYSPGAKRIAYVKFDGNDYEIYTI
                     KVGGRSETKLTHNNADEAAPCYSPDGKKIAYMVYSNATGNTEIYTIYVGGGGKTKVTN
                     GGQPSYSPDGKRIVYWDYHKTASNPHPDTEIYTINAFGGDKTQITHNNNDEFTPDYSP
                     DGKKIVYAGLEGLERNNAESDIYKIKVGGGAKTKLTNNNRYDTYPTFSPDGKKIAYTV
                     SKGKAIRGDIYTINAFGGGKTKLTHNNADEFQPSWGSRN"
     gene            11443..12114
                     /locus_tag="VFQ10_04300"
     CDS             11443..12114
                     /locus_tag="VFQ10_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9964594.1"
                     /translation="MAVVDDQRLFTKGISGLVDMLPGVEVVGVAYDGEEAVALCRKEE
                     PDVVLMDISMPKMDGISATREIKDLLPQTAVVILTGHEEDEHVFEGIKAGAQGYLLKD
                     SEPDDLSRAIHTVYAGNTIIAPDLAQKMLTTFEGGRPGGSTHLAAPPLTEREFEVIKA
                     LARGMSDRQIAKSFGISEKTVRNHTSNIYRKLHIFDRTQAVIYAVREGVIDVGDLEYR
                     PPREP"
     gene            complement(12068..13096)
                     /locus_tag="VFQ10_04305"
     CDS             complement(12068..13096)
                     /locus_tag="VFQ10_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9964595.1"
                     /translation="MDGPDKLRLLERAVTASTNSIVITDPNQPDDPIVYVNPAFERTT
                     GYAAEEVLGRNCRFLQREDRAQPALKELKAAVCEGRHCTVVLRNYRKDGTLFWNELSI
                     YSVRDEDGRMTNFVGVQNDVTERIRAEEILSEIRRAERRRIARDLHDIVLQDLSGALQ
                     SLRLTHLQAKKSRISIDFQEELGALGRASSGLRNAIYDLRHEKEQPFLESVEALVELN
                     QQATPEREMRLVIEEGFPVSLPAKESVELLRILQEALANVRRHSAARNVEVGLRTDDE
                     AILIEVADDGRGFDPGSARAGIGLSSMGERVEGLGGKIEVRSRPGEGTKVMVRVPVGD
                     GTPDPRRR"
BASE COUNT         2626 a         4333 c         4063 g         2432 t
ORIGIN      
        1 gcgttcgcct cggcccgcac cgtgccgagg aaaccgcggc gcacgggcag gttcaggaac
       61 aggtcgcgag cctccaccgt cgtaccctga gtatgggccg ccgacgcaga ctcgactatc
      121 ttcccgccga agacccgcac acgggcgccg agggaatctt cattgccgcg ttcgcgggtt
      181 gtgagcgtga gatccgagac cgctccgatg gcgtgcagtg cttcgccgcg gaagcccaga
      241 gagacaacac tggcgaggtc gtccgccgtc cggattttgc tcgtcgcgtg ctccgcgacg
      301 gcgcgctggg cgtcctcggg cgacatcccc gagccgtcgt cgctgacccg gatgagggcc
      361 actccgccgt cctcgatctc gacctcgact ctgctcgccc ccgcgtcgag ggagttctcg
      421 atcaactcct tgacggcgga cgctggacgc tcgatgatct ccccggcggc gatcctgtga
      481 gccacctcgg gcgatagcac ccgaaccagt cccgcatagc cggcggcctc ctcggagagg
      541 agcctaggaa aaagcctcat ctgatctcat ccggcgaagt ctttgacgcc ttcggcttcg
      601 aggcgggcga ggatctcacc ggcccggtcg gtgacctggg gcggtaaccc ggcgagctta
      661 gccacgtgga cgccgtagga ggaggactcc gcgccggact ctatcctgtg caggaacacg
      721 atctcacccc ccacctcctc gacgctggcc ttgtagtttc ggcagccggg caacatctcc
      781 acgagccgcg tcagctcgtg gtagtgggtg gcgaagagcg tgagggcctg cacgtcgtcg
      841 tgcaggtatt ctgcgatggc ccaagcgagg ctcatcccgt cgtaggtcga ggtgccgcgt
      901 cctacctcgt ccaggatgac caacgagcgc tccgtcgcgc tgttcaggat actcgccgct
      961 tctgtcatct ccaccataaa ggtagactca ccgctcgcga gcctgtcctc agctccgacc
     1021 cgcgtaaaga tgcggtcaat tacgccaagc gacgcctcgt cggcggggac gtaggagcct
     1081 gtctgggcca gcagggagat gagggccacc tgcctgagga agacggactt gccggccatg
     1141 ttggggcccg tgatgatctg gagccgcgag tccccgtcga cctcagagtc gttggggacg
     1201 aagggcatct ccaggttgtg ctcgacgact ggatgccttc caccaaagat gcggatgccc
     1261 ctcccttgcg tgacctcggg gcggcagtag cgcagctcgg tcgccgcagc ggcgaaggag
     1321 gagatcacat ccacgacggc tatggcgcgt gagaccttct gcagttcggg cgcggcctcc
     1381 ttgacggcgg cgcgtatctc ggcgaggatg ctgcgctcga tgcgctcgac ctcatcgcgg
     1441 gccccgagca tccgtgattc gtgctccttc aactccaccg ttacgtagcg cgcgttgtgc
     1501 ttgagcgcct tgcggtgttg gtagcgttcg gggaccgcgc tcgtctcctt gcccgccacc
     1561 tcgataaagt aaccttcccc gtccctgtag cctaccttca aggttttcag gcccgacttg
     1621 agccgctcct cggcctcgaa gcgcgtcaac cactcgtggg cgccgtccct gaactcgcgc
     1681 gcctcgtcga gctcccccga gtagccgggg cgtatgatct caccgctctc ctcggagatc
     1741 gccgctgcga ccagctccct taccccgggc ggctcctcca tcgcccctag caccctcgag
     1801 agcagcgagg agccctgcga cgccggctcc agcgtctccc tgaggggccc gatctcctct
     1861 agcgcaccct tgagcgagag cagatcgttg ggcgaggccg aaagccttac gatgcgcgtg
     1921 gcgatgcgct ccacgtcggg gatgcgtccc agatgctcgc gtacatcctc gcgcagcatg
     1981 tagtctccgg tgagcacgtc gacggccccc agacgttgcc tgatgcggtc cacttccagc
     2041 aggggccgct ccagccagcg cctgagcgct cgccggccca tcggcgtgcg ggtccggtct
     2101 acggtctcga ccagctcctc caagcccagg ttgcgcctcg tcgccgcgtc gaggaccatc
     2161 cccgatccag ggtcgtaagg gcggaaggta accacctgct ccggcggacc gccgccgcgc
     2221 agggaggcga ggtactggat caaagccccc gcggcgccag cgagcgccgg gcgcccatcg
     2281 agcccgtatc ccttgagtcc tgagacctta aagtgtctct tcagggcctg ctcgcccgcg
     2341 gaaggctcga aagtccagcg cggggcggag cttacggcgg cgcgcacctt gggcaacttc
     2401 tcggccgcgg tcctttcggg aacgacgatc tcgcgcggcg accaccgttc cagctcggcg
     2461 gggagctgct cttcagagac ctcggtgccc gtgaactctc ctgtcgaagc ctcgacgacg
     2521 gctattccag ctcggccgtc cctgacgacg aacgcggcca ggtagttggc ctgccctgcg
     2581 gagaggacgt tgtcctctat tacagtgcca ggcgtcagaa ttcgcgtgac ctcacgggtg
     2641 aactgcttgg gtttcgtcgg gtgctgccgc tgctcggcga cggcgacgga gtaacccttc
     2701 ctgagtaggg tggcaagatg ctcctgcagg gcgtgtcctg ggaccccggc cagcgggacg
     2761 cggccctcgc cggccgcctc gcggctcgtc agccggatcg agagcgcctg cgagatcctc
     2821 ttcgcgtctt cctcgaaggt ctcgaagaac gtccccacct ggtacaggag gatcgtctcc
     2881 ggcggaagtt gcgccttcag ctccgcgtat cgtccaagca tggagctatt ctacggcccg
     2941 aaccccgctt cgtcagcccc aaagtcgcac gccggcccgg gatcacaaaa cggccaccac
     3001 cgcgccaacg acgaaagcaa tatagaacag gccaccgagc attagccttc cggcccgcag
     3061 cttaccggag accctcagcg tgaggtagac gagccctccc gaagccaggg cgagcaccac
     3121 tgcgaaaagg tcaagcgggg cgaggttcca cggcgtgaaa aggacgccga aagcaacggg
     3181 tacggtgctc tggaacatca ttgcgcccgt aacattgccg agggcaaggg tgtccttgcc
     3241 ctcccttatc cagaagatgg agttgaactt ctcgggtagt tcggtcgcca gcggtgccag
     3301 tatcagggct atgaggccgg cgggcaaccc caggcccgcg gcggcgtgct cgaccgcgtc
     3361 aacgaagaag tgtgcgcccg caataatgac tccgaggctc gcgagcgtct gcaagacgac
     3421 ggcgaagtgc ggcggctcct gcctgcgcgg caggaatagt agcctcttcg gcacctcctc
     3481 gagatgctcg ccagaggata gcgttcgtct gacgtagaga ccgtagccga aagccagcac
     3541 gagagcaaca atgaccttta tgtacagcgg caactccacc agtccgacgc ccgcggccat
     3601 ggcgaagaag acaaggaaaa acccgacgtc cctgccgacg gtcgccacgt cgacgcgcag
     3661 cctcgtcccc tggtctctgc gtcgccgaaa gaagagcgtc gagactccga cgacgaacat
     3721 ggcgagggtc gcgagcatga acggtgcccc gagtatcgct cctataccaa taagtgctcc
     3781 ttcttccgac gcttcgcctg tgaggacggg ctcgaggatg gcgacaatgg cgatcatgct
     3841 ctcaggcaag gcggtaccta cggctgcgag aaggcttccc acggccccct gacgcagccc
     3901 gaggcgtcct cctagaatct ctatcgcgtt tgtaaagaag gcggccccca gaataattgc
     3961 aaccagcgcc accacaaaca ctatagccgt gaacaaaacc catctcctct cagacgggcc
     4021 gcgagacgcg aaaagcgagc caacgcggcc tgtttgcctg ccgagggtct cgctcagcgc
     4081 gccttggcgc tggtaaccgg ccgggagcct cgctccgtaa tgacgaccgg tccccccgca
     4141 acagcgggcg gctactcccc agttttttat gatcctacct gatgaatctt tgtcgttagt
     4201 gagactcttc acccatgtcg cagagaccga cgaatacgac gcacgtctac tgcttttgcc
     4261 cgctcgcgcc gggctagatg tgtccttcgg cgccattctc tgtcgcgctg tacgcggggc
     4321 tcggaaagag ataatttagc gttggtccgt aatgtggaga ggagcgagag tgagtccgaa
     4381 acctgtgatc ctggacgtag accccggcca cgacgacgcc gtggccctga tgctggcctg
     4441 cggacacccg gatctcgacc tgctcgccgt cacgaccgtc gccggcaacg tccccatcga
     4501 gaagaccacg cgcaacaccc tgcgggtgct ctccttcgtc gggtgtacgc acgtgcccgt
     4561 gggcgttggc gcgtcggaac ccctggaacg gccgctccac accgccgagg acatacacgg
     4621 caagagcggg ctggacgggg ccgaggagat ccccgacgcc ggcttcgagc ccgactacag
     4681 gggggctgtg gccctgatcg cggacaccct gcggagctct acggagccag tcaccctcat
     4741 acccgtcggc ccgctcacca acatcgccgc gttcctgcgc gagcatcctg accttaggga
     4801 cagcgtcgcg catatctccc tcatgggtgg gagcatgggc ctgggcaata cgacgccggc
     4861 cgccgagttc aacatctatg tagacccaga ggcggctcgt gaggtcttcg agtcacgact
     4921 gcccataacg atgtcggggc tcgacataac acaccaggcc ggcgccggtc ctctcgagag
     4981 gatgcggctg cgagctacgg gaagggtggg aggcgtggtg gccgggttcc tagactattt
     5041 cgccgccacc tacgagagcg ttttcggctt cgacgccccg ccgctgcacg atcccgtcgc
     5101 cgtcgcggca gtactggaac taggcctgct gaagacacgc cccatgcggg tcgacatcga
     5161 gtgcgagagc gacctcactc gcggcgagac cgtctgcgac ttctacggtg tgacaggcag
     5221 ggcgccgaac gccgaagtcg gcgtcgatct cgaccaccaa ggcttcttcg acttgctgta
     5281 cggggtgctg taaagcggtt ttcacagagg ccgccttctg aaaaccgcgc cctcagcagt
     5341 cagctttcaa cagcgcgggc cgtgcgcagc gagcggacca cgaaggcgtc gaaagttgcg
     5401 aaggcctgga tgtcttccgg ggagacttcg agtttggcgt ggtcgagcgc ccctgttacc
     5461 gtcaccctgc gctgaggcgc gacgcgctcg agcgcgtacg actgtgaggg agggaagtac
     5521 ttgtcgcgag gaccggtgac gagctcgacg gggacacgta tccgttccgt tccggcgagc
     5581 ggcgagagct cttcgaggtc gtggcgtacg tcgtcgggga gattggcgta gagcgcagcg
     5641 aagcgttccg gattacggtt cgcgatcagc ctgacgacgc tctttgcgcc cggtcccagg
     5701 tcgtccgtcc ggcgcgagct gaagccggag agcggatctg ggctctcgcg gcccgccctt
     5761 tcgagctcgg ccgcgagcgt aagcttgtcc tcgcccggcg gcagggcggc gacgagagag
     5821 cgggccacga cgtaggagag gaaggagtct gcctcgtagc gaaccagctc tcctccgggc
     5881 ctgcggtagt ggcctgtggt cgcgaggctc aggacggtct ttatgttcga atagggggcc
     5941 accccagcga cgagggagac ctttcctctg agggcgggat cctcggcggc caggagggct
     6001 agtgtggcgc ccgtcgagac ccctaccagg gctacctttc cgtcttcgac gtcgggccgg
     6061 ccggagatct cacgcgcgac ttgcgtggtg gcatcagcag tctgcggtgt gatcctagcc
     6121 tctgtcaagc caggcaggtc gggcacgacc acgaggtatc cggcccgcgc gaagccctcg
     6181 gccagatttc gcacctcagg cagttttctt ccttcccgca cggccccgtt taccaggaat
     6241 attgccggcc acggtctctc ccccgcaggc ctcacgacga gcgaggggtt cccagcgaca
     6301 cgcgcttgcg agaagcgtgg atctccgcca gcggcttcga cggctggcgt gagcacaggc
     6361 gcgtcgagga cggaggagat caccacgacg ccacgcacct gcgcgtccac ccacgaccag
     6421 tacgagatga gcagcccgat caataccgca agcaatacga gatagaaggc gcgcctgagc
     6481 cttctaccgg agagtctctt tgccgaatcc ttgccttgca acgtccctcc cgccggagac
     6541 gatctcatct accactaaac tatgcaacgc ggatcaccgc ctgtccacgg cgcgtcggat
     6601 attgttctgt tcagaaggat tccttttgta ccctcacggg taaacatcgt agacttgcgg
     6661 gcggatatca ctaaagagga ggaaactcat ggcagacctg ccacagcagg gcgagtcact
     6721 gcccgacctg gaccttacga ccgagagcgg ggaacacata ggtacgagag ggctaacggg
     6781 ccagaagacc gttctctact tctaccccaa ggacgacacc ccgggctgca cgaaggaagc
     6841 ctgcgccttc agggaccgca tggacgaata cgcaaaggcg ggtattcagg tctacggcgt
     6901 ctcgctcgac tcccccgaat ctcaccgcga gttccgcgag aagtacagcc tcaacttccc
     6961 actcctcacc gatgagggcg gccgcgcttc tgaggccctc ggggtgctac gcgaggaccg
     7021 gaagcggacg aaacgtgtca ccttcctgct cgacccggaa gccaggatct ccaaggtcta
     7081 ccccgaagtc acaccggata cccacgctga ggagatcctg gaagacgccg cctcgatata
     7141 gtctctgaag gcggagacgc cgtcattgct tggcaagcgt cagcccgcca ggagtcgggt
     7201 gaggcgcaga tgcacaccac ccgaaagttc ctgtgagccc tgacactacg ggttcgtagg
     7261 gtcgtctagt tgcagccttt agcggtagtc tttcaggaaa tccagcacga tccggttgaa
     7321 ctcttctggc ctttccatgt tcggcaggtg ggccgcaccg tgcatgacga ccttgcgcac
     7381 gttcggaagt tccctctgta gcaggtcggc tgcggcgaag acgctcggct ggtcctcgtc
     7441 tccgacaatg agcaacgtcg gagcctggac gtcagggagc cggtccgctg cgggcggctc
     7501 cggttcccac tcatcgccca gccctgaagc ttcgtttctg agggcgatca ggttcatctc
     7561 ccggaccaga tccctgacgg aggcgtctac gtcttcgggg gatctcccgg ggccgtcgac
     7621 ccagacctgg acctccagct cggagacccg ctccaggtcc ccagcctctt cggcggcgac
     7681 gatgtcgtcc cactgcctcg gggggtcgat gtcagggccg aacccgccca cggcggatcc
     7741 cacgagaacc atgtctccca ccctctgcgg atactcgagc gcgaagtcga gcacggcccc
     7801 gccgcccatc gaacagccca caagatgcgc ccgttcgacg tccatagagt cgagcagacc
     7861 gcgcaggtct tcgtagtggg agaagggtcc atccaccatc gcggtcatcc cgaagcctcg
     7921 catatcgtgg cggatgaccc tgtagcggtc ggcgaaggcc gtgagctggc cttcccacat
     7981 cctgctgtcc gcgatacccg cgtgtacgag gaccagcgga tccccttctc ccgcaacctc
     8041 atagtagatc cgcgcgccgt tgacctcggc catgcctgtc tcccgaagca tctcttccct
     8101 ttccctgata cgttcacgtc tcctccggca ccagggtcct attctgcctc gatgggacac
     8161 tcttatgaag acgctcggct gccagcacag ggccagtata cgctccccgc atctgcggca
     8221 caggaaggtt agccgcgagc gtaattcgct acgaccactg cggctgctgc tgcgcctcgc
     8281 cgtccacttc cagatccacc ttctcattga agaagcacag atggccttct atgccagcgg
     8341 ctgcgggtat gggggtcgga tagtaccaga cgattgcttc cctggtctca ccgcccgcct
     8401 gcaccgagta gtaagacgca acccccttgt agggacattc cgttttcttg tcgctaggga
     8461 gcagtacatc ctcagagact tcctcagggg gaagtagtag cgaggcggca gaccggtctc
     8521 gaagaggatc cttggacgat ccgtctcagc cacaacctcc ccatcaacgc ggatcttgac
     8581 gtgcctggag ctcttcaaga cgtcaacgcg atgatagggg tcgcggggat ggacaaagac
     8641 ctcctcgtcc tcttcgtacc agtgatctat gttgttcccc atctacgaac aatgctgggc
     8701 tgcgtttccg agccttgctg catgagagtg gcactcagag tacggaattg tcgaggttat
     8761 agagcggatt gaaaggagac gcaccctgca tctttggcga ttgaacagcg caccagcaat
     8821 gagcaggaaa gcggttcgag tccgctcgtc ggttctgcgt atccgctaat ccgcagtgtc
     8881 accgccgtgt tgtgaaagtc cttcctaatc ccggttgggc ctgtcccagt agtacacggc
     8941 gcacccgcca aacggaggga gccagaactc gtacctgggg acaacctccc cacggtacgc
     9001 tgcccttgcc cccaagacgc gtacctccat gtccttgtgc ccgatctgac agctgacgac
     9061 ctcaggctgg atggccttct catctatctc tataaaaccc gttctctcta acaacaacat
     9121 gccgacgacc agcaagagac acaccaccag cgacgccacc actattctgt atgccacgcg
     9181 gtagattatc cctctattca cccgagtgcg tgcaagtcac gttgcgccga cgatctgtct
     9241 tcgctatagt accactgtgg atatactcgc ggcgagacta tacgcagatg tagccctcgg
     9301 ggaggtcgtg cgtgggcgat agtgcacgag cacccatgcc tgagagctac ctgcccctca
     9361 ctccggcggt gcttgacatc atggtggctc tgggcaacga ggagctgcac ggctacgcca
     9421 tcatgcagga ggtcaggcgc cgcaccgatg gacaaagaca cctcgctcct ggcaccctct
     9481 accgctcgct taagcagatg gagcagaggg gatgggtggc acagtcagag gagcgccctg
     9541 agccaaacct ggacgacgag aggaggcgct actacaggct tacggagatt ggcaggaggg
     9601 tggccttggc cgaggtggag cgtcttgagg gcttggtggg tgcggcgcgt ttgaagggct
     9661 tcatcccgag tccgcgtccc tccggggcag atgcctgaga tgaggccccg tggcgcttcc
     9721 ccggcccgcg ccggttgggg agtgctaccc tcgtgctgct gctccaggag ggcggcacgg
     9781 cggcccaagc caagccgagg gcatcatgga gaaagagctg cttttagcgc tcgggcgctg
     9841 cgggaagcta acggcggtgg aggcggcgct tgagagctcg ctttcggtgg aggaggccga
     9901 acggatgcta caggagttgg cggccaaggg gcacctggag gtcagtatcg agcacggcag
     9961 gctgcactac gctctgtggg atcgcgaagc tcccctatga gggtgtttgt cgctcttgcg
    10021 taggcgagtc ctgtctttag ggaaaggagg tcgtgctcta aggaagagca tatcatgatg
    10081 ttgttatagg aacttaggcg cacagagaac agaagaggag ggggaaccac atggcaagaa
    10141 gatggacgtt ctatctaccg gtgatgatcg gggctgcggt ggcggtggca tgcgcggtgg
    10201 ctttatcggc gctctcagag aaggcggaag ctacctttcc cggcaagaac ggcaggatag
    10261 cctattcggc ccacgacggg aacgactacg agatctacac catcaactcc cgcggcgggg
    10321 ctaagcgcaa ggtgacaaac aacaataggg acgactacag cccttcctac tcgcccggcg
    10381 ccaagaggat cgcctacgtg aagttcgatg gaaacgacta cgagatctac acgatcaaag
    10441 tcggtgggag aagtgagacc aaactcaccc acaacaacgc ggacgaagcc gccccttgct
    10501 actcgcccga cggcaagaag atcgcctaca tggtgtacag caacgccacc gggaacacgg
    10561 agatctacac gatctacgtc ggcgggggtg gaaagaccaa agtgaccaac ggcggacagc
    10621 cttcctactc gcccgacggc aagaggatcg tctattggga ctatcataag acagcatcaa
    10681 acccccaccc ggacacggag atctacacga ttaacgcctt cggaggggat aagacccaaa
    10741 tcacccacaa caacaacgac gagttcactc ctgactattc gcccgacggc aaaaagatcg
    10801 tctacgcagg cttggagggc ttggagcgta ataatgccga gagcgacatc tacaagatca
    10861 aagtcggtgg gggtgctaag accaaactca ccaacaacaa caggtacgac acgtatccta
    10921 ccttctcgcc cgacggcaag aagatcgcct acacggtgtc caaaggcaag gccatcaggg
    10981 gcgacatcta cacgatcaac gccttcggag ggggtaagac caaactcacc cacaacaacg
    11041 cggacgaatt ccaaccttcc tgggggagtc gtaattgaac cgtcttgttg ccctggtcgc
    11101 cgaacggtcc ggtccctatc gtaagggtca tcgcacctat cctctcttat cggccacagc
    11161 tcgacaagag cagctggcag acccgcatct tcaggccctt atagtacacg cgcacaatgg
    11221 gtcgactgac ctactcagaa ttacccagct tggcgtggtc caaatatccg acgataacgc
    11281 tgcccgcgca gtggatcatt cgagagacag ggcactcagt atctccaccg gttcatccgt
    11341 gacacgcttg acggacagtg gatacagtta gggtcgtaac ggacttcgaa cagacaaagg
    11401 ggcgtgagac tcttgagcaa tgaaggcgaa gggattatac gcgtggcggt tgtggacgac
    11461 cagcggcttt tcaccaaggg tatttccggc ctcgtggaca tgttgcccgg cgttgaggtg
    11521 gtgggcgtgg cctacgacgg cgaggaggcc gtcgcgcttt gccgcaagga agagccggac
    11581 gtcgtcctga tggacatctc catgccgaaa atggatggca tcagtgccac gcgcgagatc
    11641 aaagaccttc tgccgcagac cgccgtagtc attctcacgg gccatgaaga agacgagcat
    11701 gtcttcgagg gcataaaagc aggcgcgcag ggctatctgc taaaagactc ggaacccgac
    11761 gatctctcgc gtgccatcca cacagtctac gccggtaaca ccataatcgc gcccgacctc
    11821 gcccagaaga tgctgacgac tttcgagggc ggcaggcccg ggggaagtac acatctcgct
    11881 gctccgccgc tgaccgagag ggagttcgag gtgatcaaag ccctcgcccg gggcatgagc
    11941 gacaggcaga tagccaaatc atttggcatc tcggagaaga cggtcaggaa ccacacctcc
    12001 aacatctaca ggaagttgca catcttcgac agaacccagg cggtgatcta cgccgtgagg
    12061 gaaggcgtca tcgacgtcgg ggatctggag taccgtcccc cacgggaacc ctaaccataa
    12121 ccttggtacc ctctccaggg cggctcctga cctcgatctt cccacccaac ccctccaccc
    12181 gctctcccat cgacgagagc ccgatgcccg ccctggccga cccaggatcg aatccccttc
    12241 catcgtcggc cacctctatc agaattgctt cgtcatccgt ccgcaacccc acctcgacat
    12301 tcctcgctgc ggagtgacgc cgtacgttcg cgagggcctc ctgcagtatg cgcaacaact
    12361 ctacgctctc ctttgcaggg agacttacag ggaacccttc ctcgatgacc agtctcatct
    12421 cacgctcggg agtagcctgc tgattcagct ccaccaacgc ttcgaccgat tccaggaacg
    12481 gctgctcctt ctcgtgcctg aggtcgtaaa tggcgttgcg cagacccgag ctcgcccgcc
    12541 ccagggctcc gagttcctct tggaagtcga tgcttatcct ggatttcttg gcctgcaggt
    12601 gggtaaggcg caacgactga agtgcgcccg agaggtcctg cagtactatg tcgtgcagat
    12661 cccgggctat cctgcgccgt tccgcccgac ggatctccga aaggatctcc tcggccctga
    12721 tgcgctcggt aacgtcattc tggaccccga cgaagttggt catgcgacca tcttcgtccc
    12781 ttacggagta gatgctgagt tcgttccaga acagggtccc gtccttgcgg tagttcctga
    12841 gcacaacggt acagtgccgt ccctcgcaga cagcggcctt gagctccttg agtgctggct
    12901 gggcccggtc ttcgcgctgc aagaaacggc aattgcggcc caaaacctcc tcggcggcgt
    12961 agccagtcgt cctctcgaag gcgggattta cgtacacgat agggtcgtca ggctggttag
    13021 ggtcggtgat cactatggag ttggtgctgg cggtcaccgc cctttcgagc aaacgcagtt
    13081 tgtcaggacc atccatagat tgccctcatc tgcgacacga ccctccacaa gtcgcccatc
    13141 aaactagcgt acggtgcaca cgctacttta tacaccttag gagcgggcgc tcgccagaga
    13201 cagcatcctc ggcgccgtga cccaggcgaa cgccgcgtct cactgcgacg aactggggcg
    13261 aaggacctag gcgcaattac ccggacacag gtagacggaa agtctgatgg cacccggaca
    13321 catccggctc atactcgttc gtgaaatgaa tgttctcgag cacgtccagc tgcgcttctt
    13381 gatcttccgg tccctttacc ggcaaagggc cgtccaaagg cttgggagcg gggtttgcac
    13441 cttcgaaccg caag
//
LOCUS       DASTKM010000104         8133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_905290, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000104 DASTKM010000000
VERSION     DASTKM010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905290"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFQ10_04310"
     CDS             complement(<1..364)
                     /locus_tag="VFQ10_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HET9964596.1"
                     /translation="MRALFLIDGEHYPPVVVDAMQSVQQSLRAEGVAAAFLGGTEKIR
                     DGFDYGVPLVEDTDPVSAVEKALADYEVDVVVDLSDEPVIGYRERMKIASLALYAGAR
                     YLGSDFELKPPDLRPVSTK"
     gene            complement(371..1195)
                     /locus_tag="VFQ10_04315"
     CDS             complement(371..1195)
                     /locus_tag="VFQ10_04315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9964597.1"
                     /translation="MSTQGRMVICQISDIHCGSPYFIPDLLERSILEINDLDPTAVVV
                     SGDLTNAGYRQEFEEAAEYVRRIQCERVMVIPGNHDSRNVGYIHFERLFGERYSILDF
                     EEAVMVAVDSSEPDLDDGRVGREHYEFIHETFDMAEDKLKIFVIHHHLIPIPGTGRER
                     NIIYDAGDVLELLADTQVDLVLSGHKHVPYAWKLENMFIVNAGTASTTRLRGNTRPCY
                     NVIEIEDGRVRVFRKYPFKDRELIVDFDSATHQYLHYEEVQSDGRAEDRRKRRTLS"
     gene            complement(1279..2106)
                     /locus_tag="VFQ10_04320"
     CDS             complement(1279..2106)
                     /locus_tag="VFQ10_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HET9964598.1"
                     /translation="MSADEDIRFVTYLSPSIPQALFEALADQVQCALGRERVSLRVES
                     RVSGPQKGGECSSFAEEADVAFMCAPSFIWLRDLQPPPVELLGVLPVFDDERNQGQPV
                     YFCDVLVRNDGPIHAFSELEGGSWAYNDACSLSGYYSLLNKLAESDLDESFFDNIFCS
                     GSHLDSIEAVLSGEADAAAIDSNVLRIRHREVPALRSKLRVIESWGPFPIQPVVVSSV
                     LPSRLKQRLRAAFLATEENQRTHQILKEFGLTRFVVVDQGDYSLDTHKDLAALLEAT"
     gene            complement(2124..3743)
                     /gene="argS"
                     /locus_tag="VFQ10_04325"
     CDS             complement(2124..3743)
                     /gene="argS"
                     /locus_tag="VFQ10_04325"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET9964599.1"
                     /translation="MAGAVREAVYRTYGVELNGIHVERPNDPAHGDFATNVALANARV
                     LRRNPREVAQNLVDALDAPFVREAEVAGPGFVNFRLSSEALWEELGALLRKGDTYGRR
                     EPSGDPVLLEFVSANPTGPLLVSHGRHAAYGDSLGRIMEAAGANVSRECYLNDGGNQV
                     RLLGESVAARYAELQDREWPVSDPEMLYRGEYVREIARDLLDERGDVYMEMPQNEALP
                     EIGAFAIRWCIEEIEQTLERSRVRFDTFFKETSLYESGAVEEVVRDLVESGNAYEGDG
                     ALWLTTSKFGDDKDRVLVKSDGSYTYMVPDLAYHRDKWDRGFRHAVDVLGADHTGYPP
                     RLRAGLVALGVPRDFLHVEIIRLVKLIRGEEQVKVSKRAGNIVTFDELLDEVGEDVAR
                     YFYVRSSHKTEMNFDLDLAIKHSEENPVFYVQYAHARISSIFERAGTGPDTLGEVPAG
                     DLAPEERLLVLELLDFPRVIQNAASRREVHPIPTYLETLATRFHQFYTVHRVLVEDAD
                     VRARRLALCAATKSVLRSGLDLVGVNSPEKM"
     gene            complement(3758..3997)
                     /locus_tag="VFQ10_04330"
     CDS             complement(3758..3997)
                     /locus_tag="VFQ10_04330"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HET9964600.1"
                     /translation="MLTYTYMRVQIDRFEDNGWAVLLPYPDGRRSFDVPREFLPEEVS
                     AGDVFEVRFEYDRDETLRIAEENRRLLDELLGGEE"
     gene            complement(4195..4668)
                     /locus_tag="VFQ10_04335"
     CDS             complement(4195..4668)
                     /locus_tag="VFQ10_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET9964601.1"
                     /translation="MIRRCTLVSVGKPKGWAAEASEDYAKRLRRYFSVNIIEVTEEDM
                     NRRSHKEVLAAEGERILKRLPKEAYVISLDREKGKQRPSEKLALTLDDLGASGRSHVA
                     FVIGGPLGLSPDVLRRSNDLWSFGSITLPHSLARVVLLEQLYRAAKIHRNEKYHW"
     gene            complement(4665..5489)
                     /locus_tag="VFQ10_04340"
     CDS             complement(4665..5489)
                     /locus_tag="VFQ10_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9964602.1"
                     /translation="MRLSVLSSGSSGNATYMETASGGLLVDAGLPPRRIGALLARIGR
                     SLADVNAVLLTHGHADHTCGVTALVRERNIEVFAAPGVGESLGANTVAPGEPCSVEDL
                     GVTFFEVPHDSMTYGVRVSDGSSVVAMATDLGEAGVNVLRSMSGAEAVVLEANHDPDW
                     LRRGPYSASLKRRISSRRGHLSNRQAADAAVALAPQGLKDLVLAHLSKTNNSPTRACG
                     TVDRALREAGHGSVRVRAAIAGHPTPWIEVGAPLLVPEHVYRYGERGTAGQLFGIE"
     gene            5721..6308
                     /locus_tag="VFQ10_04345"
     CDS             5721..6308
                     /locus_tag="VFQ10_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9964603.1"
                     /translation="MAERGRHYLILADEDLISLVEAADAEAFATLYDRHSGAAFSLAY
                     RMMGERQASEDLTQDAFLKVWRSASSYRAERGSVRTWILSIVHNRGIDQIRSQASRRR
                     TQDKIEASAPRSQPSEAFVETLRNSQRDQVREALDTLPPEQLKILELAYFSGYTHVEI
                     SDLLRLPLGTVKGRMRLGLKKIRDYFESRDAAVPR"
     gene            6305..7078
                     /locus_tag="VFQ10_04350"
     CDS             6305..7078
                     /locus_tag="VFQ10_04350"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET9964604.1"
                     /translation="MSEMNHERFEDLKDAYVLGALPEEERLSFEDYLAAHPERQAEID
                     ELGAVAGLLAFSPQEQEPPPELRRSVMEVVGAEAEPRRVRGRSTFAKVGDYLSVRSLA
                     LGAAALLVIGLLSWNVLLQSQVEVLQGRVQDAQGRVQDLQAQVENARDQRQQSPTVEL
                     EGSWADQGANAKVVSIQENQVILVARNMPSVPEDQTCQIWVINDDVPKPSGLFQPDRN
                     ITAAPITNSITNADVIAVTVEPAGGSKKPTSDPVLLANL"
     gene            7280..7486
                     /locus_tag="VFQ10_04355"
     CDS             7280..7486
                     /locus_tag="VFQ10_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HET9964605.1"
                     /translation="MSVARVTELSVISETSFEDAIQRGIARATSTLRGVEGAWIKDQN
                     VMVEDGNITGYKVNMEITFVLEDS"
     gene            7729..>8133
                     /locus_tag="VFQ10_04360"
     CDS             7729..>8133
                     /locus_tag="VFQ10_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9964606.1"
                     /translation="MYANPDLREIRDVLQSSDTVAVVGLSDRPYRTSYAIAGALQGFG
                     FKIFPVNPNLRGPVLGEEPYESVKNVPTQVDIVDVFRRSGKVMPVAEDAVAAGASVLW
                     MQSGVINEEAAAYAEEHGLIVVMDRCIKVDYAT"
BASE COUNT         1563 a         2443 c         2545 g         1582 t
ORIGIN      
        1 gcttggtaga gacgggacga agatccgggg gcttcagttc gaagtcgctg ccgaggtagc
       61 gcgccccggc gtagagggcg agcgaggcta tcttcatccg ctctctgtag ccgattacgg
      121 gctcgtcgga gaggtcgacg acaacgtcaa cctcgtagtc ggcgagcgcc ttctctactg
      181 cggagacggg gtctgtgtcc tcgaccagcg ggacgccgta gtcgaagcca tcccttatct
      241 tctccgtgcc cccgaggaag gccgctgcca caccttcggc tcgaagggac tgctgcacgc
      301 tttgcatagc gtccacgacg acaggaggat agtgctcgcc atcgatcagg aacagcgccc
      361 gcacggggtt ctagctcagc gttcgccgct tgcgacggtc ctctgctctg ccatcgctct
      421 ggacctcttc gtagtgcagg tactggtggg tcgcggagtc gaagtccacg atcagctcgc
      481 ggtccttgaa cggatacttg cggaagaccc tcacccttcc gtcctcgatc tcgatgacgt
      541 tgtagcaggg gcgcgtgtta cccctgaggc gggtggtcga ggccgtgcct gcgttgacga
      601 tgaacatgtt ctcgagcttc cacgcgtaag gcacgtgctt gtgcccggag agtacgaggt
      661 cgacctgcgt gtcggcgagc aactccagca catcccccgc atcgtagatg atgttgcgct
      721 cccttcccgt tcccgggatg gggatcaggt ggtggtggat aacgaagatc ttgagcttgt
      781 cctcggccat gtcgaaggtc tcgtggatga actcgtagtg ctcgcggcca acccgcccgt
      841 cgtcgagatc gggctcggat gagtccacgg cgaccatgac ggcttcttcg aagtccagaa
      901 tagagtaccg ttcaccgaag agccgttcga agtgtatata accgacgttg cgcgaatcgt
      961 ggttgcccgg tatcaccatc acgcgctcgc actggatgcg ccggacgtac tcggccgcct
     1021 cctcgaactc ctggcggtaa cccgcgttcg tcaggtcacc cgacaccacg acggcggtag
     1081 gatccaggtc gtttatctcg aggatcgaac gttcgagcaa gtcgggtatg aagtacggac
     1141 tgccgcagtg gatgtcgctg atctgacaga taaccatcct gccctgcgtg ctcattcact
     1201 ccccgattct attcacttaa ggcgccgccg gaagtatagc agagcgcctt cgatgccccg
     1261 caggctacag gtatcgggct atgtggcctc gagaagtgct gcgaggtcct tgtgggtgtc
     1321 gagggagtag tctccctggt caacgacgac gaagcgggtg aggccgaact ctttcaggat
     1381 ttggtgtgtg cgctggttct cttctgtggc gaggaaggcg gcgcgcagtc gttgtttcaa
     1441 ccgtgagggt agtacagagc tgaccacgac gggctgtatc gggaacgggc cccaggactc
     1501 gatcacgcgc aacttgctgc ggagggccgg cacctctcgg tgtcgtatcc tcaagacgtt
     1561 cgagtcgatc gcagcggcgt ccgcctcgcc gctaaggacc gcttctatgg aatcgaggtg
     1621 agaccctgag cagaatatgt tatcgaaaaa gctctcgtcc aagtctgact ccgccagctt
     1681 gttcagcagg ctgtagtagc cactcaacga gcacgcatcg ttgtacgccc atgagcctcc
     1741 ttcgagctcg gagaaagcgt ggatcggacc gtcgttgcgc accaagacgt cacagaagta
     1801 gacgggctgc ccctggttcc tttcgtcgtc gaacacgggc aggacgccga gcagttcaac
     1861 gggcggcggt tgcagatcgc gcaaccagat gaaagaagga gcacacatga acgccacgtc
     1921 ggcttcttcg gcaaaggagg agcattcgcc gcccttttgc ggtcccgaga cccgcgactc
     1981 taccctgagc gagacccgct cgcgtccgag ggcgcactgc acctggtcag ccaacgcctc
     2041 gaagagggcc tgtgggatac ttggcgagag gtaggtgacg aagcggatgt cctcgtccgc
     2101 agacatcatt gctctaccgc accttacatc ttctctggcg agttcactcc gaccagatcg
     2161 aggccgctgc ggagcacgga cttagtggcg gcgcaaaggg cgaggcggcg ggcgcgaacg
     2221 tcagcgtctt cgaccaggac gcggtggacg gtatagaact ggtggaaacg cgtggcgagg
     2281 gtctcgaggt aggttgggat gggatgcacc tcgcgcctgg aggctgcgtt ctgtatgaca
     2341 cgggggaagt cgaggagctc gagcacgagg agacgctcct cgggtgcgag gtcccctgca
     2401 gggacctcgc ccaaagtatc aggacccgtt cctgcccgct cgaaaatact agatatcctg
     2461 gcgtgggcgt actgcacgta gaagacaggg ttctcctcgg agtgttttat ggcgagatcc
     2521 aggtcgaagt tcatctccgt cttgtgggag gagcgcacgt agaagtagcg cgccacgtcc
     2581 tcccccacct cgtcaagcag ctcgtcgaag gtcacgatgt tgccggcccg cttgctgacc
     2641 tttacctgct cttccccacg aatcagcttg acgagccgta ttatctccac gtgcaggaaa
     2701 tcccttggta cgccaagcgc gacgaggcct gccctgaggc gtggcggata gccggtgtga
     2761 tcggcgccaa gcacatccac agcgtgcctg aaacctcgat cccacttgtc gcggtggtag
     2821 gcgagatcgg gcaccatata cgtgtaagat ccatcgctct tgacgagcac tctgtcctta
     2881 tcgtcaccga acttcgaggt cgtcagccag agggctccat cgccctcgta agcgttgcca
     2941 ctctccacca ggtcccgcac gacttcctct accgcgcccg actcgtagag actcgtttcc
     3001 ttgaagaacg tgtcgaagcg gacgcgagag cgctccaggg tttgctctat ttcctcgatg
     3061 caccagcgga tggcgaacgc gccgatctcc gggagagctt cattctgcgg catctccatg
     3121 tagacgtctc cgcgttcgtc caggagatca cgcgcgatct cccgcacgta ttccccgcgg
     3181 tagagcattt cagggtcgga gacgggccat tcccgatctt ggagttctgc gtagcgcgcg
     3241 gcgacggact caccgagaag ccgtacctgg ttgcctccgt catttaggta gcactcccgc
     3301 gaaacgtttg cgcccgcagc ctccatgatg cggccaagcg agtcaccgta ggccgcgtgc
     3361 cgaccatggg acactagcaa cgggcccgtc ggattggcgc tcacgaactc gagcagtacg
     3421 gggtctccgg agggctcccg ccgcccgtac gtgtcaccct tgcgaagcag agcgccaagc
     3481 tcttcccaga gcgcttccga cgagagcctg aagttgacga agcctggtcc tgcgacttcg
     3541 gcctcccgca cgaaaggggc gtccagggcg tccaccaggt tctgagcgac ttctctcgga
     3601 ttccgtcgca ataccctcgc attggcgagt gctacgttgg tcgcgaagtc gccgtgagcg
     3661 ggatcgttcg gccgctcgac gtgaataccg ttcagctcga caccgtaggt cctgtagacg
     3721 gcttcccgaa ccgcgccagc caaacgctcc tcgaagctca ctcttcaccc ccgagaagct
     3781 cgtcaagaag ccgccggttc tcttcggcga tccgcagggt ctcatccctg tcgtactcga
     3841 aacggacctc gaaaacgtcc cccgcggaga cctcttcagg caggaactcg cgcggtacgt
     3901 cgaagcttct cctcccgtcg gggtaaggca agagcacggc ccaaccattg tcctcgaacc
     3961 tgtctatctg cactcgcata tatgtgtatg ttagcacgct gcctccggca gcttgtcagc
     4021 ttttcagcac gccgcctttc aggcggcttg tcagcacgct acgcccttcg ggcttcgctt
     4081 ctcagcacgg cgcttcgcgc ctcttagcac ttcagcaacg gcttgcatac gcgtaaaacc
     4141 gaaatgttga caagcgcagg cgtcagccgc agcgtgctga cgtgtgaagc ggagctacca
     4201 gtgatatttc tcgttacggt gtatttttgc cgcgcggtag agctgttcga ggagaacgac
     4261 cctggcgagg gagtgcggga gtgtgatgct gccgaaggac cagaggtcgt tggatctacg
     4321 cagtacatca ggggagaggc cgagcgggcc gccgatcaca aaggcgacgt gactccggcc
     4381 tgaggcgccg aggtcgtcca gtgtcagggc gagtttctct gatgggcgct gctttccttt
     4441 ctccctgtcg agggagatca cgtacgcttc tttcgggagg cgcttcagga tacgctctcc
     4501 ctctgcggcg agcacctctt tgtgggagcg gcggttcatg tcttcctcgg tgacctcgat
     4561 gatgtttaca gaaaaatagc ggcgcagtcg ttttgcgtag tcctcagagg cttcggcggc
     4621 ccagcccttt ggtttcccga cggagacgag ggtgcagcgg cgtatcactc gataccgaag
     4681 agttgtcctg ccgtgccccg ctccccgtag cggtagacat gctcgggcac cagcagcggg
     4741 gctcctacct cgatccaggg tgtcggatgg ccggcgatcg ccgcccgaac cctgacggag
     4801 ccgtggcctg cttccctgag cgcccggtct acggttccac aggcccgggt cggagagttg
     4861 ttggtcttgg agaggtgggc gaggacgagg tctttgagcc cctgcggagc cagcgccacg
     4921 gcggcatcgg ccgcttgccg gttcgaaagg tggccgcgcc tcgaggagat ccggcgcttc
     4981 agactcgccg agtatgggcc acgcctgagc cagtctggat cgtggttcgc ctcgaggacc
     5041 accgcctcgg ctccactcat gcttcgcagt acattgactc cagcctcacc gaggtccgtc
     5101 gccatcgcca cgacgctact tccatcggat acccgaaccc cgtaggtcat cgagtcgtgt
     5161 gggacttcga aaaacgttac tccaaggtct tcgacggagc agggctctcc tggggccacc
     5221 gtgttcgcgc cgagggattc gccgacgccc ggcgcggcga agacctcgat attgcgttct
     5281 cgcaccagag cagtgacacc gcaggtgtgg tcggcgtgtc cgtgggtgag caggacggcg
     5341 tttacgtcgg ccaagctcct gccgatacgg gcgaggagag cgcctatgcg ccgtggcggg
     5401 aggcctgcgt ctacgaggag gccgcctgat gccgtctcca tgtaggtcgc attgcccgag
     5461 cttccgctcg aaagtacact cagccgcaaa tccgtcctct atcgggttgg gcgtttgatc
     5521 atcgggaacg aagcccgttt agatcttagc acgcccaatc caggtgctct tgtcgacgag
     5581 ctcgccctcg acgattcagg tgcttttcgt aatctggcag cgtgcaagtg tcactacttg
     5641 attcttccga tgggctgttt gcggtacttt ggcagagggg gcgttgatcc ggactggcgg
     5701 tattgccgta gaatcagggc atggctgaaa ggggacgaca ctaccttatt ctcgcggatg
     5761 aggatttgat ctccctggtc gaggcggccg acgccgaggc gtttgcgacg ctctacgacc
     5821 gccacagcgg cgcagccttc tccctggctt acaggatgat gggggagcgt caggcctccg
     5881 aagacctgac gcaggatgcg ttcttgaagg tctggcggag cgctagcagc tatcgtgcgg
     5941 agcgaggcag cgttaggacc tggatcctct ccatcgtcca caaccgcggc atagaccaga
     6001 tccgttccca ggcaagccgg cgcaggacgc aggacaagat cgaagcctca gccccacgct
     6061 cccaacccag cgaggctttc gtggagaccc taaggaactc gcaacgcgac caggtcaggg
     6121 aggcacttga caccctccca cccgagcagc tgaagatact ggagctggcg tacttttccg
     6181 ggtacacgca tgtggagata tccgaccttc tccgcctgcc tctcggaacg gtcaagggca
     6241 ggatgcgttt gggactgaaa aagatcaggg actacttcga gtcaagagac gcggcggtgc
     6301 ccagatgagt gagatgaacc acgaacgctt cgaagatctc aaagacgcct acgtcctcgg
     6361 tgccttgccc gaggaggaga ggctgagttt cgaagattac ctcgccgccc accctgaaag
     6421 gcaggccgag atagatgagc ttggcgccgt ggctggcctg ctcgcgttct cccctcaaga
     6481 acaggaaccg ccgccggagc tgcgcagaag tgtcatggag gttgtcgggg ccgaggcgga
     6541 gccccgccgc gtcaggggca ggtccacgtt cgcgaaggtc ggggactatc tcagcgtccg
     6601 gagcctcgcc ctcggcgccg cggccctgct cgtgatcggg ttgctatcct ggaacgtgct
     6661 attgcaaagt caggtagaag tcctacaagg ccgggttcag gatgcgcagg gccgggtaca
     6721 ggacctgcaa gctcaggtcg agaacgcgcg ggatcagcgg cagcagagcc cgacggtcga
     6781 gctcgaggga tcgtgggcgg atcagggcgc caatgccaaa gtcgtttcca tccaagagaa
     6841 ccaggtcatc ctggtcgcca ggaacatgcc ttctgtgccc gaggaccaga cgtgccagat
     6901 ctgggtcata aacgacgacg tgcccaaacc cagcggactc ttccagcctg acaggaacat
     6961 tacagcggcg ccgatcacga actccataac gaatgccgac gtgatagcgg tgaccgtcga
     7021 accggcagga ggttcgaaga agccgacgag cgatcccgta ctactggcca acctctaagc
     7081 ggctacaggc accaggatcc agggcggcag gcgatgcaca ctacctcaat ggtccgaccg
     7141 atcttgtagg tggggttagg tacgtaccta acgctcgtct cgcggcctgg gcgcctctgt
     7201 cggggagcgt ttattttgtg cgggcgcggg tagtaaatgc tcagggaagc agagagcaag
     7261 ctagggcaaa ggagagcata tgtccgtagc cagagtaacc gaactgagcg tcatctcgga
     7321 gacgagcttc gaggatgcta tccagcgggg catagcccgg gccacttcca ccctgcgcgg
     7381 tgtggaggga gcctggatca aggaccagaa cgtcatggtc gaagacggga acatcacggg
     7441 ctacaaggtg aacatggaga tcaccttcgt cctggaagat tcgtaagggc cacagctagg
     7501 cacaatgtat tgccccggct ctcaggccgg ggttttgtct tggtggcttg cttcgcaaac
     7561 gttctaggca tgcgtggaca gcagggactg gcaagcgctg ctcggcgtca ttggcggcag
     7621 agcggccgac gttatggtcg aggccaaggg caaagagcac gcccttgcgc cgatgggcgt
     7681 cgagatcgta taacatcggg acctgaggtc cgtgaggaag agagcgacat gtacgcaaac
     7741 cctgacctta gagagataag agatgttctg caaagctcgg acaccgtggc tgtcgtcgga
     7801 ctttcggaca gaccttaccg caccagctac gcgatcgccg gcgcactgca gggcttcgga
     7861 ttcaagatct tccccgtcaa cccgaatctc cgtggtcctg tgcttggcga agagccttac
     7921 gagtcggtca agaacgtccc cactcaggtg gacatagtcg acgtgttccg gcgtagcggg
     7981 aaggtcatgc cagtggccga agacgccgtg gccgccggcg cgagcgttct gtggatgcaa
     8041 tcaggcgtta tcaacgaaga ggccgccgcc tacgccgaag aacacggcct catcgtcgtc
     8101 atggaccgct gtataaaggt agattacgcg acg
//
LOCUS       DASTKM010000105        18328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_552812, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000105 DASTKM010000000
VERSION     DASTKM010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 18328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18328
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552812"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..108)
                     /locus_tag="VFQ10_04365"
     tRNA            complement(27..108)
                     /locus_tag="VFQ10_04365"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72..74),aa:Tyr,seq:gta)
     gene            complement(209..280)
                     /locus_tag="VFQ10_04370"
     tRNA            complement(209..280)
                     /locus_tag="VFQ10_04370"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Thr,seq:tgt)
     gene            complement(587..1087)
                     /locus_tag="VFQ10_04375"
     CDS             complement(587..1087)
                     /locus_tag="VFQ10_04375"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9964607.1"
                     /translation="MALILDGYNLIGALDRYGVAGSLDAARDLLINDALKAAGWTGRP
                     LIVVFDAHRGTEAERVESRAGGAVRVIYSAPGESADDVIERLLSRLEGSATVYTADFA
                     LQRTALARGATRATPREFMNLLDELPAITRSPDVRRKSRLADRLSLETLHRLEEIRRR
                     AADGDA"
     gene            complement(1078..1788)
                     /gene="rlmB"
                     /locus_tag="VFQ10_04380"
     CDS             complement(1078..1788)
                     /gene="rlmB"
                     /locus_tag="VFQ10_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET9964608.1"
                     /translation="MKQETVYGKRPVVEALRSGRRKVFEVIDAVGDEEVANAAGARGV
                     SVKRASRQRVEELAWGGVHQGVAARVEPYPYSGLEEILSIPDPLVLVLDGVTDPRNLG
                     AVLRAADGAGASGVVIPKDRAVGVRAPAVKASAGASEHVLVARVTNLRRAVDTMKEAG
                     LWIYAAEAGGTPYTELDLAAAVALVLGSEGRGVRRLVREGCDGTVSIPMLGGVESLNV
                     SVASAVLLYEARRRRGWP"
     gene            complement(1789..3207)
                     /gene="cysS"
                     /locus_tag="VFQ10_04385"
     CDS             complement(1789..3207)
                     /gene="cysS"
                     /locus_tag="VFQ10_04385"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET9964609.1"
                     /translation="MSLLVKDSMSGKMVEVGESGRLGLYICGPTVYDHIHVGNARAPL
                     FWDVVARYLRSRGYDVTFVQNITDIEDKIINRANQEGVSWQEIVDRYTDSFHERLEML
                     GIGLPDIEPHATEHIPEMISLIEELIENGHAYAPEEAKGDVYYAVGSFPQYGALSHQR
                     PEEMRETEKGQTGFKRSPLDFALWKASKPGEPSWESPWGRGRPGWHIECSAMVSKHLP
                     EGADIHGGGTDIRFPHHENELAQSAAAHPNQPFVRAWAHHGMVTLTSGKMAKSVGNVL
                     DVTRAVNLHGRNAVRMWLLQSHYSQPIEYSEEILEEKRRSFERLMRLYLQISGSQSSS
                     GLSDTLAADLRESVEDAMKDDLNTPEAMAALFEVAGRAGREMSSRPEAAGEFGSLAEA
                     IEEVMTIFGFDLAQERSTGVEGVNIRYSGDPGAEILNQVATREKSRREKDWDTADRLR
                     EELHAEGWTVEDTSEGPILSRR"
     gene            complement(3204..4514)
                     /locus_tag="VFQ10_04390"
     CDS             complement(3204..4514)
                     /locus_tag="VFQ10_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HET9964610.1"
                     /translation="MPAGTALSGVRVRFAPSPTGLLHLGAARTALFDYLFARHHGGDL
                     ILRIEDTDRERSRPEFEHAQLEDLEWLGLSFDEGPYRQSERGELYEGAARRLIDAALT
                     YESTDESGRRALYFRPPVRSGSFVDELRGEVTFGGVEDFVILKSDGTPSYNFAVVVDD
                     LELGITHVIRGEEHLSNTARQALLYRALGATEPRFIHLGVILGPDGKKLSKRHGAASI
                     ADYRREGYVPEALVNYLALLGWNHPEGKEEFAGLHEISREWDPSRLGTSPATFDPDRL
                     LFFNANHIRRLPAEELLRRLEPFLEESLPGGREPAALEAIREDMRTLSEASHLLRAIT
                     GPVNPDVLVEELQGASDEVFAHAAAGLEGREFGDVEEARDFVAGLRAWAEERGIKTRD
                     LLHPLRLALTGKNRGPEMAYLFAVLGAEEARERILRAREARLGS"
     gene            complement(4493..4972)
                     /gene="ispF"
                     /locus_tag="VFQ10_04395"
     CDS             complement(4493..4972)
                     /gene="ispF"
                     /locus_tag="VFQ10_04395"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET9964611.1"
                     /translation="MRVGLGVDVHAFAAPEDGRRLVLGGVEIPSDRGLLGHSDADVLA
                     HAVADALLGAAGLEDIGHYFPDTDERYRDADSIGLLRDVRGLVGESWAVANVDAVVIC
                     ERPKIRDHREAMRKNLAAALGTEESRVGVRGTTTERLGFPGRGEGIAAQAVCLLERR"
     gene            complement(4972..5655)
                     /gene="ispD"
                     /locus_tag="VFQ10_04400"
     CDS             complement(4972..5655)
                     /gene="ispD"
                     /locus_tag="VFQ10_04400"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET9964612.1"
                     /translation="MLAGGTGRRMGRPKQFIELLGMPALHYTMQAFEESPEVATIYAV
                     GDRQRIEALVQAAGITKYAACAEPGEARSLSTRNGLHLCAEDPQTIVLVHDGSRCLVT
                     PGVIKRVIRAAHGASEPDGVIPGVPVSDTIKVAREGNVAETLDRTNLWAVQTPQAFRL
                     GLLRRLHDLPDEFLRAATDDASLVERDGGRVEIVAGEKTNIKLTSPEDLVFAEAILAV
                     REGAREAMP"
     gene            complement(5682..6767)
                     /gene="disA"
                     /locus_tag="VFQ10_04405"
     CDS             complement(5682..6767)
                     /gene="disA"
                     /locus_tag="VFQ10_04405"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HET9964613.1"
                     /translation="MSPRRRHLPAELARALELVAPGTDLREAIENVMRAHNGALIVLD
                     KPERLESLGIISGGMKVEVEFAPMRLYELAKMDGAIVVSPDISAIHYANVQLSPDPSL
                     ESEETGMRHLAAHRTAQQTGSLVIAVSERRRVVSLYQGSYGPHVLEDIGVVLSKANSA
                     IATLEKYTRRLREEARALTVHEYDGAVTLREVVGAVGTFEYSARIAEEIEAYVRELGS
                     EGRLVEMQLEQAFHGVPEQYEALLRDYVAEEADDRVVREELRTLSAEQLSDSAEIARA
                     LGYGPDGQLEELFIKPRGYRQLERVPRLPRRVAESLIRAFGSLEEILDATEEELDEVE
                     GVGQARARTIQRGLERQRSLETTGEVS"
     gene            complement(6764..8158)
                     /gene="radA"
                     /locus_tag="VFQ10_04410"
     CDS             complement(6764..8158)
                     /gene="radA"
                     /locus_tag="VFQ10_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET9964614.1"
                     /translation="MATKTLYVCSNCGHAEPKWLGRCPDCGEWSTFVEEMRESKKVVG
                     FAERATRQKAGVAGRTLALSEVPVVEGGGRMDAGVGELNRVLGGGIVPGSMVLVGGEP
                     GVGKSTLLLQVMGHLGEGCLMVSGEESPRQVALSARRLGVEGRGFRVLSETDVDIIEA
                     TILQERPRAVVVDSIQTLYSPELSGAPGGVGQVRECAARLMRLAKSEGIAVVLVGHVT
                     KEGSIAGPRVLEHMVDTVLQFEGDRFQSFRVLRALKNRFGSTNEVGVFEMTGRGMVEV
                     EDPSAFFLSKREEEVPPGVATVCLLEGTRPMLVEIESLVAPSPLANPRRIAKGVDTGR
                     VNMLCAVLSRRAGLALQDQDVYVNVTGGVRVEEPAADLGVALAIASAMRDRPVGKGTA
                     CFGEVGLTGDVRYVSGAQRRTSEMAKMGFGRIIRPESTSDGPPDNETGGGSRRESSVV
                     EVSTLEEAVAVALL"
     gene            complement(8307..9542)
                     /locus_tag="VFQ10_04415"
     CDS             complement(8307..9542)
                     /locus_tag="VFQ10_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET9964615.1"
                     /translation="MSGANLPLEGVTVVSLEQAVAAPFATRQLADLGARVIKVERPGV
                     GDFARGYDETVMGMSSHFVWLNRSKESLALDLRQDEAQEVLRRLVARADVFVQNLAPG
                     AAGRLGFGAEVLREEHPRLVYCSISGYGEDGPYTEKKAYDLLVQCEAGLISITGTPET
                     PSKVGISAADIAAGMYAYSGILSALYRREKTGEGATLEVSMLEALGEWMGFPAYFAGY
                     GGEEPRRSGAAHAAIAPYGPFGCGDGEVIFLGIQNEREWKKFCEEVLGREGMAEDHRF
                     DKNSRRVENRDGLHAEIESVFSGLSSEEVIERLEGAGVANARMRTVRQFLDHPQLEAR
                     NRWREFGSPVGSLRGLLPPATLDGAEPVMSPIPEVGEHNEKLLAELGYGETRDLGSSG
                     GSSRSLGSQSKEPSKRLPS"
     gene            complement(9539..9745)
                     /locus_tag="VFQ10_04420"
     CDS             complement(9539..9745)
                     /locus_tag="VFQ10_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964616.1"
                     /translation="MTGHSGTATLPGMSVEFLLTSLVVVLIPGTGVVYTSTIGGGRRH
                     GLFAPVRWAGVAYFLNAGPDGYLP"
     gene            9751..9993
                     /locus_tag="VFQ10_04425"
     CDS             9751..9993
                     /locus_tag="VFQ10_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964617.1"
                     /translation="MPLELPRSPLRPRLGGEGSIQAEIGWIYMCVWLAVAIGLVVFDW
                     KAWRGPAPAPATTALSVPPGEATQAAHQEEEQTIGR"
     gene            complement(10138..12648)
                     /locus_tag="VFQ10_04430"
     CDS             complement(10138..12648)
                     /locus_tag="VFQ10_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET9964618.1"
                     /translation="MFERFTERARKVVVLAQDEARHFNHNYIGTEHLLLGLLREDEGV
                     AAQALYSLNVALDEVREQVESIVGYGEEGTGAQAPFTPRSKKVLELALREALQLGHNY
                     IGTEHILLGLVRESEGVAARVLSNLDVDPDKVRREVVRRLGGGRSRSRGGGESGGGRG
                     VEAKRPKTRQLDQYGRNLTAYAEEDKLDPVIGRSQEIARIMQILVRRTKNNPVIIGEP
                     GVGKTAIVEGLAEEIAEGKVPEILGDKEVYTLDLGALVAGSKYRGEFEERLKKIMKEI
                     TDHGDIILFIDEIHNLVGAGAAEGAIDAASILKPALARGEIQVIGATTIDEYRKYVEK
                     DKALERRFQTIQVGEPSVEETELILKGLREKYETHHNIDITDEALKAASQLGDRYISD
                     RFLPDKAIDLVDEAASKMRIKTMSSPPYYKEIDEELAAVRSEKEAAIDGQEFEKAARF
                     RDSERKLVAQRRELEKAWREGKIEEKRVSIGENEIAEIVSMWTGIPVKKMTEEESARL
                     LQMEDALHGRIVGQNEAIKAVSRSIRRTFAGIKDPNRPSGSFVFLGPTGVGKTELART
                     LAEYLFGNQDAMIRLDMSEYMERHTVSRLVGSPPGYVGYDEGGQLTEQVRRRPYSVVL
                     FDEIEKAHPDVFNIMLQILEDGQLTDAQGRTVDFKNVVLIMTSNVGAQTINKTKSLGF
                     GSDDSGLSYKEMKGRVTSELRKIFRPELLNRIDEIIVFHKLEREDMRHIIEIQIKRLR
                     QQLVERDVTIEFTDEALDKLAEEGYDPAFGARPLKRVLQRMIEDPMSEMILRGEVPNG
                     SKVIIEPNDKTSEEINEEESIVDIKVTQPKEIVKVESE"
     gene            complement(12971..14446)
                     /gene="lysS"
                     /locus_tag="VFQ10_04435"
     CDS             complement(12971..14446)
                     /gene="lysS"
                     /locus_tag="VFQ10_04435"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF014031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET9964619.1"
                     /translation="MSETSRSTGIHIPGWAERVADSLGAGPHVVVSGISVSGNIHAGN
                     LREVLVAEAVANALRQRGEEVRFIFHADTIDPLRKIAPGIPKSYEEYIGHSLSHIPDP
                     EGCHVSYAEHFLVPFEEALREMEMDIEVLRSHELYERGVYTGVTSEAIENTDELREIL
                     QEVTNRKMPEHWSPYLPRAESGKLTGMRVLEHLPEQTSVVFADEDGWQDSADYSRGEG
                     KLGWRVELAARWKALGVTFEPFGKDHTSRGGSTDTADRMSREVFHYPVPGRYEYEWIG
                     LKGRGDMSSSRGIVLLPKDLLKIMPPGAVRRIILGRDPARRFDIDLEGGFPRFMDEYR
                     AETGEPYVPFTHLVTVAQTVGEDTDAAAQMLRRGGYEEAVGEGLADDLYYARNWAAEW
                     APESMRLGLLDLAESEQAAASLDDEQREYLREVSARLRAEMDGEAIQEVLYSTAIERG
                     LKPKRAFAAVYRVLLGKTSGPKAGPFIAGLTLDQVSERFSV"
     gene            complement(14511..14999)
                     /gene="greA"
                     /locus_tag="VFQ10_04440"
     CDS             complement(14511..14999)
                     /gene="greA"
                     /locus_tag="VFQ10_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET9964620.1"
                     /translation="MTDKNQELVTPEGLEKLQQELAYLTETRRKEVANRIRQAREFGD
                     ISENSEYDEAKNEQGLLERRIGELQRRVRNVKVVDPSEVEDDAVDLGTRVTLRVAGRE
                     GQERTFQIVGANESDPTSGKLSHASPVGRAVLKRKVGEKVMVSTPRGATEYEIVNVEA
                     AS"
     gene            complement(15160..15924)
                     /locus_tag="VFQ10_04445"
     CDS             complement(15160..15924)
                     /locus_tag="VFQ10_04445"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET9964621.1"
                     /translation="MAVDVGNTQTVLGLIDGGEPRGQWRIATEAHRTADELAVTFAGL
                     LALEGLSPHEVSAMIVSSVVPGLARSYRNLASEVFGVPYYAVTAEMETGLYNRYDDPG
                     AVGADRIVNAVAAVRHYGVPVIIVDIGTATTVEAVDGEACYLGGAILTGLYVSLEALV
                     SKTAKLPSVDLEEEPPRVIATNTPDSIRSGFIYGYAGAVDALIRRSRDELGEDGVKVV
                     ATGGPAGVIVPYCREIQEFDPDLTLKGLLALYEMNA"
     gene            complement(15924..16427)
                     /gene="folK"
                     /locus_tag="VFQ10_04450"
     CDS             complement(15924..16427)
                     /gene="folK"
                     /locus_tag="VFQ10_04450"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET9964622.1"
                     /translation="MTRAFLSLGSNLGDRMAYLRAVVAALDRGPRMRIKGTSKVYETI
                     PVEVDEEQPDYLNCVVELECGVPAIELLRYCQGVEAALGRERSGAAEKAPRTVDIDVL
                     LFGEQTITEPELSVPHAGVTRAFNLRGLADLDADLYIPGCGPVLELLGRAEGGGILKF
                     GEADDLC"
     gene            complement(16424..17284)
                     /gene="folP"
                     /locus_tag="VFQ10_04455"
     CDS             complement(16424..17284)
                     /gene="folP"
                     /locus_tag="VFQ10_04455"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET9964623.1"
                     /translation="MGRTARNTDRAGNLTAGPGPVVVGILNVTPDSFSDGGNFLHPEA
                     AAEHAAAMLDEGAGMLDVGGESTRPGSDPVSQEEEIQRVVPVVERIISERPEAIISVD
                     TYRSGTATAALQAGAKFVNDVTAFRGDPSMASVIKEAACPVILMHMQGEPKTMQKEPR
                     YEDVVREVRDFLAERAEYAVSAGVRPENIIVDPGIGFGKNLQHNLALLRNLDAIVDLG
                     FPVLIGASRKSFIERITGVQEPRDRVSGTVATTVLAYERGATFFRVHDVRANREALAV
                     AEAVLHTSRR"
     gene            complement(17285..>18328)
                     /locus_tag="VFQ10_04460"
     CDS             complement(17285..>18328)
                     /locus_tag="VFQ10_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9964624.1"
                     /translation="EQTLNQLLVEMDGFDSKSGIIMLAATNRADILDPALLRPGRFDR
                     QIVVDRPDLPGREKILRVHTRGKPLGPDVEIGTLARGTPGFTGADLANLVNEAALLAA
                     RHDKNQIDMSEMEEAIDRVIAGPERKTRLISDKEKEITAYHEGGHAIVGALLPDADPV
                     HKITIIPRGQALGVTMSLPTEDRFMMSRGQLMAQLAMMLGGRAAERVVFDEITTGASN
                     DLERVTQTARQMVTRFGMSEKLGPMALGHQQGQVFMGRDFNNQPDYSDEIAFQIDKEI
                     RRIVDESYDTAEDVLVRNRELLDKLSAELIEYETVDANHLKRLIEQYAVDSISPNGAV
                     PDSDGRYRGSYQD"
BASE COUNT         3006 a         6124 c         5618 g         3580 t
ORIGIN      
        1 agctacaggg gcatattccg tctgtgtggg ggcgggagga ttcgaacctc cgtaggcgaa
       61 gccggcagat ttacagtctg ctccctttgg ccgctcgggc acacccccgg ccacgccccc
      121 gcggcactct gtgcggagcg ctcgaatatg gtagcaaacg ccaccgactt ttcaacagat
      181 gagaaggact gcagggacct ccaaggagag cccgggaagg gattcgaacc cccgaccggc
      241 cgcttacaag gcggctgctc tacctctgag ctacccgggc gcggcgccgc aggcgccgcg
      301 ccgcacgctg gcggctgcgc cagctggtca gcacgctccg gcctacggcc ttcgcttgtc
      361 agcacgttcc ggcctccact ctgagcccgc ttcgggcctt cggccctcgc tttcagccaa
      421 tagcccttca ggaccagctc gccggcacgt accagggatt gtaacagcgt gaaccccgca
      481 ccgacatagc cattgttcct tgctgaaagc ggagcccgtc ggggcgaagc gtgctgacaa
      541 ggcgcgaagc accgtgctga caagcggagg cgttcgccgg agcgtgctaa gcgtcgccat
      601 cggcggcgcg ccggcgtatc tcttccagcc tgtgcaaggt ctcaagcgat agccggtctg
      661 ccaaacggga cttgcgccgg acgtcggggc tgcgggttat ggcaggtaac tcgtccagga
      721 ggttcatgaa ctcccgcggg gtggcgcggg tcgcgcctcg ggcgagggct gtgcgctgca
      781 gggcgaagtc agccgtgtag actgtggctg agccctccag tctggagagc aggcgttcga
      841 tcacgtcgtc cgccgactcc cctggggcgc tgtagatcac ccgcacagct ccgccggccc
      901 gtgactcgac cctctcggcc tcggtacccc ggtgcgcgtc gaagactacg ataaggggcc
      961 ttcccgtcca ccccgccgcc ttcagggcgt cgtttatcag gaggtcgcgg gccgcgtcga
     1021 ggctcccggc caccccgtaa cggtcgaggg cgccgatgag gttgtagccg tccaggatca
     1081 gggccacccc cgcctcctcc ttgcctcgta gagcaatacc gcggaagcga cggagacgtt
     1141 tagagactcg acgcccccta gcatggggat ggagacggtc ccgtcgcacc cctcgcgcac
     1201 gagccgccgc actccccgcc cctcgctgcc tagcacgagc gccacggccg ctgcaaggtc
     1261 gagttcggta taaggcgtcc cgccggcctc ggctgcgtag atccagaggc cggcctcctt
     1321 catcgtgtct acggcgcgcc tcaggttcgt cacccttgcg accagcacgt gctcgctcgc
     1381 cccagcgctc gccttgaccg cgggagccct cacccctacg gccctgtcct tgggaatgac
     1441 gaccccgctg gccccggccc cgtcagcggc ccgcagcact gccccgaggt tgcgcgggtc
     1501 ggtgacaccg tcgagcacga gaaccagagg gtcagggatg gagaggatct cctccagtcc
     1561 ggaataagga taaggttcga cgcgggctgc cacgccctgg tgaaccccgc cccaggccag
     1621 ctcctctacc cgctggcgtg aggcgcgctt tacgcttacg ccgcgcgctc cggcggcgtt
     1681 cgcgacctcc tcgtcaccta ccgcgtcgat gacctcgaag acctttcgtc ttccgctcct
     1741 gagagcctcg accacgggcc ttttgccgta gacggtctcc tgtttcaatt agcggcggct
     1801 gaggatcgga ccttcagaag tatcctccac ggtccaccct tcggcgtgga gctcttcgcg
     1861 cagccggtcg gcggtatccc agtccttctc acgcctggac ttctctcttg tggccacctg
     1921 gttcagaatc tcagcgccag ggtccccaga gtacctgatg ttcacgcctt ccacacccgt
     1981 ggatctctcc tgcgcgaggt cgaacccgaa gatagtcatg acctcctcta tggcctcggc
     2041 gagcgaaccg aactcgcccg ctgcctcggg gcgactcgac atctcacgtc ccgcccttcc
     2101 ggcgacctcg aagagagccg ccattgcctc gggagtattc aggtcgtcct tcattgcatc
     2161 ttcgacgctt tcgcgaagat ccgctgccag cgtgtcagac agacccgagg aactttgcga
     2221 accggagatc tggaggtaaa gacgcatgag ccgctcgaag gagcggcgct tctcctcgag
     2281 gatctcctct gagtactcga tgggctggga gtagtggctc tggaggagcc acatccgcac
     2341 ggcgttgcgg ccgtggaggt tcacggcccg cgtcacgtcg agcacgttcc cgacggattt
     2401 agccatcttt ccgctggtga gcgtcaccat accgtgatgg gcccaggcgc ggacgaaggg
     2461 ttggttaggg tgggccgcgg cgctctgggc gagctcgttc tcgtggtgcg ggaagcgtat
     2521 atccgtgccg ccgccgtgga tatcggcccc ttctggcaga tgcttgctca ccatcgcgga
     2581 gcactcgatg tgccagccag ggcgcccccg gccccagggg ctctcccagg agggctcgcc
     2641 cggtttggag gccttccaca gcgcgaagtc cagcgggctc ctcttgaagc ctgtctgacc
     2701 cttctccgtc tcgcgcatct cttcgggacg ttggtggctg agcgcgccgt attgcgggaa
     2761 gcttcctacg gcgtagtaga cgtcgccttt ggcttcctct ggggcgtagg cgtggccgtt
     2821 ctctatgagc tcttcgatca gggagatcat ctcggggatg tgctcggtgg cgtgcggctc
     2881 gatgtcaggc agccctatgc ccagcatctc cagccgttcg tggaaggagt ccgtgtagcg
     2941 gtctacgatc tcctgccagg agactccctc ctggttggcc ctgtttatga tcttgtcctc
     3001 tatgtccgtg atgttctgga cgaacgtcac gtcgtaaccc cggctcctca ggtacctggc
     3061 gaccacgtcc cagaacaagg gcgcccgcgc gttgccgacg tggatgtgat cgtagaccgt
     3121 aggaccgcag atgtagaggc cgagcctgcc gctctccccc acctcgacca tcttgccgct
     3181 catgctgtcc tttacgagca agctcatgac cctagtctag cctctctagc ccgaagtatc
     3241 ctctccctgg cctcctcggc acccaacacc gcgaagaggt aagccatctc gggccccctg
     3301 ttcttgcccg tgagcgctag cctcagaggg tgtaggagat ccctggtctt tatcccccgc
     3361 tcctcagccc acgcgcgaag cccggctacg aaatcacgcg cctcctccac atccccgaac
     3421 tcccggcctt ccagcccggc ggcggcgtgc gcgaagacct cgtcgcttgc gccctgcaac
     3481 tcctctacga gaacgtcagg gttcacgggg cccgtgatcg cacgcagcaa gtgcgacgct
     3541 tcggagaggg tcctcatatc ttctcttatg gcctcgagtg ccgcaggctc ccgcccgcct
     3601 ggcagcgact cttcgaggaa aggctcgagg cgcctgagaa gctcctcagc aggcagacgc
     3661 cggatgtggt tggcattgaa gaacagcaga cggtcggggt cgaaggtcgc cgggctcgtt
     3721 cctaggcgtg aagggtccca ttcgcgcgag atttcatgga ggcccgcgaa ctcttccttc
     3781 ccttccggat ggttccagcc gagcagggcg aggtagttca ccaacgcttc agggacgtat
     3841 ccttccctgc ggtagtcagc gatgctcgcg gcgccgtggc gtttggagag cttcttgccg
     3901 tcaggcccga ggatgacccc gaggtggata aaccggggtt cggtcgcgcc gagcgccctg
     3961 tagagcaagg cctggcgggc ggtattggag agatgttcct cgccccggat cacatgcgtt
     4021 atccccagct ccagatcgtc gacgaccacg gcgaagttgt aagaaggcgt gccgtcggac
     4081 ttgaggatca cgaagtcctc tacgccgccg aacgtcacct ctcccctcaa ctcatcgacg
     4141 aagctcccgc tacgcaccgg cggacggaag tagagagcgc gccgccccga ttcgtccgtg
     4201 ctttcgtagg tgagagcggc atctatcaga cgccgggcgg ctccctcata gagctcccca
     4261 cgttcgctct ggcggtatgg gccctcgtcg aaagacaatc cgagccattc gaggtcttcg
     4321 agctgcgcgt gttcgaactc cgggcggctc cgctccctgt cggtgtcctc gatacggagg
     4381 ataaggtctc ccccgtgatg cctggcgaag aggtaatcga agagcgcggt gcgggccgcg
     4441 ccgaggtgga ggagccctgt agggctaggg gcgaagcgga ccctgacccc gctcaacgcc
     4501 gttccagcag gcacaccgcc tgggcggcta tgccctcgcc cctacccggg aagccgagtc
     4561 gctcggtcgt ggtgccgcgt acgccgacgc gggactcttc tgttccgagg gcggcggcga
     4621 ggttcttccg catggcttcc ctatggtccc tgatcttcgg ccgctcacag atgacgacgg
     4681 cgtccacgtt ggcaaccgcc cacgattccc caacgagccc gcgaacgtcg cgcagcaacc
     4741 cgatggagtc ggcatccctg taacgctcgt ccgtgtcggg gaagtagtgg ccgatgtctt
     4801 ccagacctgc cgccccgaga agggcgtccg caacggcgtg ggcaagcacg tcggcatcgg
     4861 agtggccgag gaggcctcta tcggagggga tctcgacccc cccgagtacg agcctgcgac
     4921 catcctccgg cgcggcgaag gcgtggacgt cgacaccgag cccgacccgc atcaaggcat
     4981 ggcctcacgg gcaccctcgc gcacggcgag tatggcctcg gcgaatacca gatcctcagg
     5041 ggaggtcaac ttgatgttgg tcttctcccc cgcgacgatc tccacacgac ctccatcccg
     5101 ctcgaccagg gaggcatcat cggtcgcggc acggaggaac tcgtcaggca agtcgtggag
     5161 gcggcgcagc aggccgaggc ggaacgcctg gggagtctgg accgcccaca ggttcgtcct
     5221 gtccagggtc tcggcgacgt tgccttccct ggcgactttg atggtgtcag agacggggac
     5281 ccctggaata acgccgtcag gctcgctagc tccatgtgcc gcccttatga cgcgctttat
     5341 gacgccgggc gtgaccaggc aacgagaccc gtcgtgcacc agtacgatag tctgcgggtc
     5401 ttccgcgcag aggtgcaggc cgttcctggt cgagagggag cgggcctcgc caggttcggc
     5461 gcaggcggcg tacttggtga taccggcggc ctgcacgagg gcttcgatcc tctgcctgtc
     5521 gccgacggcg tagattgtgg cgacttcggg cgactcttcg aaggcctgca tcgtgtagtg
     5581 gagggccggc atgccgagga gttcgatgaa ctgcttgggg cggcccatcc tgcggcctgt
     5641 gccgccggcg agcaccagcg cgatcgctga tccccggtcc tctatgagac ctcgcctgtg
     5701 gtctccaggc tacgctgacg ttcgaggccg cgctggatgg tgcgggcgcg ggcctggccg
     5761 accccctcta cctcgtcgag ctcctcctcc gttgcgtcga ggatctcttc gagagagccg
     5821 aacgccctga tgaggctctc tgcgaccctg cgcggcaggc gtggcacccg ctctagttga
     5881 cggtatccgc ggggtttgat gaacaactcc tccagctggc cgtccggacc gtagccaagg
     5941 gcacgggcga tctctgccga gtcagagagt tgctcggccg atagtgtcct gagctcctcc
     6001 ctgacgaccc tgtcgtctgc ctcttccgcg acgtagtctc tcagcaaggc ttcgtattgc
     6061 tcgggaacgc cgtggaaggc ttgctctagc tgcatctcga ccagccgacc ctcgctgcca
     6121 agctccctca cgtaagcttc tatctcctcg gcgatcctgg ccgagtactc gaaggttccc
     6181 acggcgccga cgacctcccg cagcgtcacg gccccgtcgt actcgtgaac ggtcagggcc
     6241 cgcgcctcct ccctcagcct cctggtgtac ttctcgagcg tcgcgatagc cgagttcgcc
     6301 ttggacagca ctaccccgat atcctccaga acgtgcggcc cgtaggagcc ctggtagaga
     6361 ctcaccaccc tcctacgctc ggagaccgct atgacgaggc tgcccgtctg ctgcgccgtg
     6421 cggtgagccg ccaggtggcg cataccggtc tcctcagact cgagggaggg gtctggacta
     6481 agctggacgt tcgcgtagtg gatggcggag atatcgggcg agaccacgat cgcgccgtcc
     6541 atcttcgcca gctcgtagag gcgcatcggc gcgaactcca cctccacctt catcccgccc
     6601 gagatgatac cgaggctttc gagcctctcg ggtttgtcca ggacgatcag ggccccgttg
     6661 tgcgccctca tgacgttctc tatggcctcc ctgagatccg taccaggagc cacgagctcg
     6721 agggcgcggg ccagctccgc aggcagatgc cgccgccggg gactcacagc aatgccaccg
     6781 ccacggcttc ctccaaggta cttacttcca cgacgctcga ctctctccgg gacccgcctc
     6841 cggtctcatt gtccggcggg ccatccgacg tactttccgg cctgattata cgaccgaagc
     6901 ccatcttggc catctcggag gtccggcgct gggcgcccga gacatagcgc acgtcccccg
     6961 tgagcccgac ctcgccgaaa cacgcagtcc ccttgcccac aggtctgtcc cgcatcgccg
     7021 aagcgatagc gagcgccacc ccgaggtcgg ccgccggctc ttcgacccgc acgccaccgg
     7081 tgacgttgac gtacacgtcc tgatcctgga gtgccaggcc cgccctcctg ctcagcaccg
     7141 cacacagcat gttgacccgc cctgtgtcca cgcccttggc tatgcgccgc gggttggcga
     7201 gcgggctcgg tgctaccagg ctctctatct cgaccagcat cggccgcgtc ccctccagaa
     7261 gacagaccgt agcgacgcca ggcggcactt cctcctccct cttcgagagg aagaatgccg
     7321 aagggtcctc cacctcgacc atcccgcggc ccgtcatctc gaagaccccc acctcgttag
     7381 tcgacccgaa gcggttcttg agggcccgca agaccctgaa gctctggaac cgatccccct
     7441 cgaactggag caccgtgtcg accatgtgct ccagtacgcg agggcccgct atggacccct
     7501 ccttcgtcac gtgcccaaca aggaccaccg ctatgccctc gctcttggcg agccgcatga
     7561 gcctcgccgc gcactcgcga acctgcccga ctccgcctgg cgcccccgag agctcgggcg
     7621 agtatagcgt ctggatcgaa tccactacca cggccctggg ccgctcctgg agaatggtcg
     7681 cctcgataat gtccacgtcc gtctcggaga ggaccctgaa gccccgccct tcgaccccga
     7741 gtcgccgggc gctcaaagcc acctgcctcg gcgattcctc gcctgagacc ataaggcagc
     7801 cttcccccag gtgtcccata acttgaagaa gaagcgtgct cttgccgacg ccgggttccc
     7861 ccccgacgag gaccatcgag cctggtacga tgccacctcc tagaaccctg ttcagctccc
     7921 ctaccccagc gtccatcctg cccccgccct cgactacggg cacctcgcta agggccagcg
     7981 tcctacctgc gaccccggcc ttctgcctcg tcgcccgctc ggcgaaaccg acgaccttct
     8041 tgctctcgcg catctcctcc acgaaggtac tccactcccc gcaatcaggg caacgtccga
     8101 gccacttcgg ttcggcgtgc ccgcaattgg aacaaacata aagagtcttc gtagccatga
     8161 tccgattgta ctggatcgaa gaaaaagtac agtaaacaaa gggcgattgg ggtcatctca
     8221 catcctacat taccctctca cagcaaactg gttaagccct gattaactcg gtaccaagcg
     8281 gcaagatccc acagaggcca gcgcagttac gagggtaagc gcttggaggg ctccttggat
     8341 tgagacccca aggagcgact ggaacctcca gaagagccca ggtctcgtgt ctccccgtag
     8401 ccgagctccg cgaggagttt ttcgttgtgc tccccgacct cggggatggg gctcatgact
     8461 ggctccgcgc cgtccagggt agcagggggg aggagcccac gtaacgaacc cactggcgag
     8521 ccgaactcgc gccagcggtt tctggcctcc agctgggggt gatcgaggaa ctgccgcacg
     8581 gtgcgcatcc tggcgttcgc cacccccgcc ccttcgagcc tctctatgac ttcctccgag
     8641 gagaggcctg agaagacgct ttctatctcg gcgtgcaacc cgtcgcggtt ctcgacgcgc
     8701 ctggagtttt tgtcgaagcg gtggtcttca gccatcccct cgcgccccag gacctcctca
     8761 cagaacttct tccactcgcg ctcgttctgt atcccgagga agatgacttc gccatcgccg
     8821 cacccgaacg ggccgtaagg cgctatcgcc gcgtgtgcag cgccgctgcg ccggggctcc
     8881 tcaccaccgt acccggcgaa gtacgccggg aagcccatcc actctcccaa cgcctccagc
     8941 atcgagacct cgagcgtcgc accttcgccc gtcttctcgc gtctgtagag ggcggagagg
     9001 atgccggaat aagcgtacat tcccgcggcg atgtcagcgg cggagatgcc gaccttggag
     9061 ggtgtttcag gcgtgcccgt gatggagatc agccccgcct cgcactgcac gagcaggtcg
     9121 taggccttct tctcggtgta aggcccgtct tcgccgtagc ccgagatgga gcagtacacc
     9181 agccgcgggt gctcttccct cagcacttct gcaccgaaac cgaggcgtcc ggccgcgcca
     9241 ggagcgaggt tttggacgaa gacgtcggcc ctggccacga ggcggcgaag aacctcttgg
     9301 gcctcgtcct gccttagatc gagggccagc gactccttgg agcggttgag ccagacgaag
     9361 tgacttgaca tgcccatgac cgtttcgtca taaccccggg cgaagtcccc tacgccgggg
     9421 cgctcgacct ttatgaccct cgcgccgaga tccgcgagct ggcgggtggc gaagggggcg
     9481 gcgaccgcct gctccaggga gactacggtc accccttcga gaggtaggtt cgcgccactc
     9541 atggtaagta accatcaggc ccggcgttta ggaagtaggc gacccccgcc catcggaccg
     9601 gggcgaacag accgtgcctc cggccgccgc cgatagtgct cgtgtagaca accccggtgc
     9661 ccgggatgag gaccacgacg agcgacgtca gcaggaactc gacggacatg ccaggcagcg
     9721 tagccgttcc ggaatgaccc gtcaagaaca ttgcctctcg aactcccccg gagccccctg
     9781 cgcccccgtc ttggaggaga agggagcatc caggccgaga tcggctggat ctatatgtgc
     9841 gtgtggttgg ccgtggcgat aggcctcgtc gtcttcgact ggaaagcctg gcgcggacct
     9901 gcgcctgcgc ccgcgacgac ggcgctctcg gtaccgccag gagaagcgac acaggcggcg
     9961 caccaggagg aggaacaaac cataggacgg taatccgaag caggaacaag ggcgctgagg
    10021 gatctgtccg ggaccgaatt caccctggtg atgctacgcg agaatccgag tagccccgca
    10081 tgaaaggccg ggacgccata gacgccccgg ctcctgtgtg cggtagtttg gttttcctta
    10141 ctcagattct accttgacga tctccttggg ctgggtgacc ttgatgtcga ctatggactc
    10201 ttcctcgttt atctcttctg aggttttgtc gttgggctcg attatgacct tggagccgtt
    10261 ggggacctcg ccgcgcagga tcatctcgct catggggtct tcgatcatgc gctgcagcac
    10321 acgcttcaag gggcgcgccc cgaaggcggg gtcgtagcct tcctcggcga gcttgtcgag
    10381 ggcctcatcg gtgaactcga tcgtaacgtc gcgctcgaca agctgctggc ggaggcgctt
    10441 tatctggatc tcgattatgt ggcgcatatc ctcgcgctcg agcttgtgga acacgatgat
    10501 ctcgtcgatc ctgttgagga gctcgggacg gaagatcttg cgcagctcgc tcgtgacgcg
    10561 gcccttcatc tccttgtagg agagaccgga atcgtcagag ccgaagccca aagacttggt
    10621 cttgttgatg gtctgcgccc cgacgttgga ggtcatgatg aggacgacgt tcttgaagtc
    10681 cacggtgcgg ccctgcgcgt ctgtgagctg tccgtcctcc agaatctgca acatgatgtt
    10741 gaagacgtca gggtgggcct tctcgatctc gtcgaagagg actaccgaat aaggtcgtcg
    10801 gcgcacctgt tcagtgagct gtccgccctc atcgtagccg acgtagccag gaggcgaacc
    10861 gacgagacgt gagaccgtat ggcgctccat gtactcggac atgtcaagcc ggatcatggc
    10921 gtcctggttg ccgaagaggt actcggcgag cgtcctcgcg agctcggtct tgccgactcc
    10981 ggtaggaccg aggaagacga atgagccgct ggggcggttg gggtctttta tgccggcgaa
    11041 ggtacgcctt atggaacggg agacggcttt tatggcctcg ttctgcccga cgatgcgccc
    11101 gtgcagggcg tcctccattt gcaggagcct cgctgactcc tcctcggtca tcttcttgac
    11161 cgggatgcct gtccacatag agacgatctc ggcgatctcg ttctcaccga tggagacgcg
    11221 cttctcctcg atcttaccct cgcgccaagc cttctcgagc tcgcggcgct gggcgacgag
    11281 cttgcgttcg gagtcccgaa agcgcgcggc cttctcgaac tcctggccgt cgatggcggc
    11341 ctctttctcg gagcgcaccg cggcgagctc ctcgtctatc tccttgtagt agggcggcga
    11401 ggacatggtc tttatcctca tcttggaggc tgcctcgtcg acgaggtcga tggccttgtc
    11461 aggcaggaag cggtcagaga tgtagcggtc gccgagttgc gaggcggcct tgagcgcctc
    11521 gtcggtgatg tctatgttgt gatgtgtctc gtacttctcg cgcagcccct tgaggatgag
    11581 ctcggtctcc tcgaccgagg gctcgcccac ctggatggtc tgaaagcgcc gctccagggc
    11641 cttgtccttc tccacgtact tgcggtactc gtctatcgtc gtagcaccga taacctggat
    11701 ctcaccccgc gccagcgcag gcttgaggat ggaggcggcg tctatggcgc cctcagcggc
    11761 tcccgctccg accagattgt ggatctcatc gataaacagg atgatgtcgc cgtggtctgt
    11821 gatctccttc attatcttct tgaggcgctc ctcgaactcg ccgcggtact tggagcctgc
    11881 gacgagggcg ccgaggtcga gcgtgtagac ctctttgtcc cccaggatct cgggtacctt
    11941 accctctgcg atctcctcgg ccagcccctc gacgatggcg gtcttgccga cgccgggctc
    12001 accgatgatt acggggttgt tcttggtgcg cctgacgagg atctgcatga tcctcgctat
    12061 ctcctggctc cggccgatca cagggtccag cttgtcctcc tcggcgtagg ccgtgaggtt
    12121 cctgccgtac tggtcgagct gacgggtctt gggccgcttg gcctcgaccc ctcgaccgcc
    12181 gccggactct ccaccgcctc ggctgcggct gcggccaccg ccgagcctcc tgactacctc
    12241 ccgcctcacc ttgtcagggt cgacgtcgag gttggagaga accctggctg cgacgccctc
    12301 gctctccctc accagcccga gaaggatgtg ctccgtgcct atgtagttgt ggccgagctg
    12361 cagcgcctct ctgagcgcga gctctaggac cttcttggag cgtggcgtga acggggcctg
    12421 cgcgcccgtg ccttcctcgc cgtagccgac gatgctctct acctgttcgc gcacctcgtc
    12481 gagggcgacg ttcagggagt agagggcctg ggcagcgacg ccctcgtcct cgcgaaggag
    12541 tccgaggagc aagtgctcgg tgccgatgta gttgtggttg aaatgacggg cctcatcctg
    12601 tgcgagcacc acgaccttgc gggcccgctc cgtgaaacgt tcgaacatta gcgggaatct
    12661 cctctgtttc gagggtcctt cacggcatcg gggatcataa cggtctgtat ctgtatacga
    12721 gtatcgaaga tcgacggatt acacatctct aaaaatctgc ctttcgaaat aacgtgcgcc
    12781 tctcctgata gtacctctct atagctcgct ccggccaacc taagtataca accgttgggc
    12841 tggttttacg gtacccgctt ggagctttac gctctaagcg ctatacctcg tgttgcggtg
    12901 ggccggctca tcctcttgta gcgctttatg gaagactatc gtagtcctct caccgatatg
    12961 gtgtctcgtt ttacacggag aacctctcgc tgacctgatc cagggtcaat cctgcgatga
    13021 aggggccggc tttgggcccg ctggtcttgc ccagcaagac cctgtagacg gcggcgaacg
    13081 ccctcttggg cttgagtccg cgctcgattg cagtcgaata gagtacttcc tggatggcct
    13141 ctccatccat ctcggcccgg agcctcgccg agacctcgcg caggtactcg cgctgctcgt
    13201 cgtcgagcga cgcggccgcc tgctccgact cggccaggtc gagcaacccc agccgcatcg
    13261 actcgggagc ccactcggca gcccagttgc gggcatagta gagatcatcc gccagccctt
    13321 caccgaccgc ctcctcgtac ccgccacgcc gcagcatctg cgcggccgcg tcagtgtctt
    13381 cgccgacggt ctgggcgacg gtgaccagat gggtgaaggg gacgtagggc tcgcctgtct
    13441 cggcgcggta ctcgtccata aagcgcggga agccgccttc caggtcgata tcgaagcgac
    13501 gggcggggtc gcggccgagg atgatgcgcc tgacggcgcc tggtggcatt atcttcagga
    13561 gatccttcgg caggagcacg ataccccgag agctgctcat gtccccgcgg cccttgagcc
    13621 ctatccactc gtactcgtag cggcccggca ccgggtagtg gaagacctcg cgggacatcc
    13681 gatcagcggt gtcggtcgag ccaccgcgac tggtgtggtc cttgccgaaa ggctcgaagg
    13741 ttaccccgag cgccttccac cgggccgcga gctcgacccg ccagccaagc ttgccctcgc
    13801 ccctggagta atcggcgctg tcctgccagc cgtcctcatc cgcgaagacg acgctggtct
    13861 gctccggcaa gtgctccagg acgcgcatcc cggtaagctt ccccgattcc gcccggggca
    13921 ggtagggcga ccagtgctcg ggcatctttc gattcgtgac ctcctgaagg atctcgcgca
    13981 actcgtctgt gttctcgatg gcctcgctgg ttaccccggt gtagacgccc cgctcgtaca
    14041 gctcgtgcga gcggagcacc tctatgtcca tctccatctc tctgagcgcc tcttcgaacg
    14101 gcacgagaaa atgctcggcg taggagacat ggcagccttc ggggtccggt atgtgcgaga
    14161 ggctatggcc gatgtattct tcgtagctct tggggatgcc cggggctatc ttgcgcaacg
    14221 ggtcgatcgt gtccgcgtga aaaatgaaac ggacctcctc gccgcgttgc cgcagagcgt
    14281 tcgccaccgc ctcggcgacg aggacctcgc ggaggttgcc agcgtggatg ttaccggaga
    14341 cgctgatgcc gctcacgacc acgtgcggcc ccgctcccag agaatcggcg accctctcgg
    14401 cccacccggg tatgtgaatg ccggtcgacc tgctggtctc gctcatgcgg tactccaatt
    14461 gggttcggtg gttggaatcg gggagaagcg ggtcgcgatc tcccgacgcc ctagctggcc
    14521 gcctcgacgt tgacgatctc gtactccgtc gcgccgcggg gtgtggagac catgaccttc
    14581 tcccccacct tcctctttag tacggcccgg cccaccgggg aggcgtggct gagcttcccg
    14641 ctcgtcgggt ccgactcgtt ggcgccgacg atctggaacg tccgctcctg tccctctctg
    14701 cctgcgaccc tgagggttac ccgagtacca agatcgaccg cgtcgtcctc gacttccgaa
    14761 gggtcgacaa ccttcacgtt gcgcaccctg cgctggagct caccgatccg gcgctccagc
    14821 aagccctgtt cgttcttcgc ctcgtcgtac tcggagttct cagagatgtc cccgaactca
    14881 cgggcctggc ggatgcggtt ggcaacctcc ttgcgacgcg tctcggtcag gtatgcgagt
    14941 tcctgctgga gcttctcgag accttccgga gtaacaagct cctggttctt atcggtcata
    15001 agccgcgaga ttataagggc gggggcaagg ggtgtcaacg aggaaacgct gcgcgtttcc
    15061 gcaggctccg gcctttcagg cctccgcttg tcagcacttc agcacgcttc gggcctgcgg
    15121 ccctcgcttg tcagcattgg cgtgctggct gaattgctct cacgcgttca tctcgtagag
    15181 cgccagcagg ccctttaacg tgaggtcggg gtcgaactcc tgtatctcgc ggcaataggg
    15241 cactatgacc ccggccggac cacctgtggc gacgaccttg acgccgtcct cgcccagttc
    15301 gtcacgggac ctcctgatca gggcgtcgac agcgcctgcg tagccgtaga tgaaaccgct
    15361 gcggatggag tcgggagtat tggtggctat gaccctcggt ggttcttctt cgaggtccac
    15421 gctgggaagc ttggccgttt tggagactag cgcttccagc gaaacgtaga ggcctgtcag
    15481 tatggcaccc ccgaggtagc atgcctcgcc gtcaacggcc tcgacggtcg tcgcggtgcc
    15541 gatatcgacg atgatcacgg ggaccccgta gtgcctcaca gccgccacgg cgttgacgat
    15601 cctgtcggcc ccgacggccc cggggtcgtc gtagcggttg tagaggccgg tctccatctc
    15661 ggcggttacc gcgtagtagg gtaccccgaa gacctcggag gcgaggttac ggtaggaacg
    15721 ggcgagaccg ggcacgacgc tggagacgat cattgcgctc acctcgtggg ggctcaaacc
    15781 ttcgagcgcg aggagcccgg cgaaggtgac ggcgagctcg tcggccgtcc tgtgagcctc
    15841 cgtggcgata cgccactgtc cccgcggctc cccaccatcg atgagcccga ggacagtctg
    15901 ggtattcccg acgtccacag ccatcaacaa aggtcgtcag cctctccgaa cttcaggatt
    15961 cctccccctt ccgctcttcc aagcaactcc aggaccggtc cgcaccccgg aatatacaga
    16021 tccgcatcaa ggtccgcgag ccccctgagg ttgaaggcac gcgtcacccc cgcgtgcggc
    16081 accgagagtt caggttccgt tatggtctgt tctccgaaca gcaacacgtc tatatccacc
    16141 gtgcgtgggg ccttctcggc ggccccgctg cgctcgcgcc cgagcgcggc ctcgacaccc
    16201 tggcagtagc gaagcaactc gatcgcggga acgccacact ccagctcgac cacgcaattc
    16261 agatagtccg gctgctcctc gtcgacctcg acaggtatcg tctcgtagac tttcgacgta
    16321 cctttgatcc tcatccgagg cccccgatcg agggcggcga ccacggccct caggtaggcc
    16381 atacgatcac ctagattact gccgaggctc aggaatgcgc gagtcaccgg cgggaggtgt
    16441 ggagcacggc ctcggcgacc gcgagcgctt cgcggttggc gcgcacgtcg tgcaccctga
    16501 agaaggtcgc gccccgttcg taggcgagca ccgttgtggc gaccgtgccg gagacgcgat
    16561 ctctcggctc ttgtacgcct gtgatcctct ctataaagct cttgcgcgag gccccgatca
    16621 ggacggggaa accgagatct acgatagcgt caaggtttcg caggagcgcc aggttgtgct
    16681 ggaggttctt cccgaagccg atgccggggt ccacgattat gttctcgggc ctgacgcccg
    16741 ccgagacggc atattcggcc ctctcagcaa ggaagtccct gacctcgcgc accacatcct
    16801 cgtagcgggg ttccttctgc atggtcttcg gctcgccttg catgtgcatc aggatcacgg
    16861 ggcacgccgc ctcctttatc acggaggcca tgctggggtc gccacggaac gcggtaacgt
    16921 cgttgacgaa ctttgcaccg gcttggaggg ctgccgtggc ggtgcctgac cggtaggtgt
    16981 cgaccgagat aatcgcctcg gggcgctcgg agatgattct ctcgacaaca gggacgacgc
    17041 gttgtatctc ctcctcctgc gagaccgggt ccgaaccggg gcgggtggac tctccaccga
    17101 cgtccagcat gccggccccc tcgtcgagca tggcagcggc gtgctctgcc gcggcttcag
    17161 ggtggaggaa gttcccgccg tcggagaagg agtcaggggt tacgttcagg atgccgacga
    17221 cgacagggcc gggacctgcc gtaaggttcc cggccctgtc tgtgttccgg gccgtgcggc
    17281 ccatctagtc ctggtagcta cccctgtagc gtccgtcgga gtcggggacg gcaccgttcg
    17341 gtgagatgct atccacggcg tactgctcga tcaggcgctt caggtgattg gcgtctacgg
    17401 tctcgtactc gatcagctcg gcggagagct tgtccagcag ctcacgatta cgaaccagta
    17461 catcctcggc ggtgtcgtag gactcatcga cgatcctgcg gatctccttg tctatctgga
    17521 aggcgatctc gtctgagtaa tccggctggt tgttgaagtc gcggcccatg aagacctgtc
    17581 cctgctggtg ccccaaggcc atgggtccta gcttctcgct catcccaaag cgcgttacca
    17641 tctgcctggc cgtctgcgtc actcgttcga ggtcgtttga ggcgccggtt gtgatctcgt
    17701 cgaagaccac cctttcagcg gccctaccgc cgagcatcat ggcgagctgt gccatcagct
    17761 ggccccggct catcatgaac ctatcctctg ttggcaggct catggttaca ccgagcgcct
    17821 ggccgcgcgg gatgatggtg atcttgtgca cagggtcggc atcaggaagg agcgctccta
    17881 cgatggcgtg cccgccctcg tggtaggctg ttatctcctt ctccttgtcg gagatgaggc
    17941 gggtcttcct ctccggaccg gcgatcacgc ggtctatggc ctcttccatc tctgacatgt
    18001 cgatctggtt cttgtcgtga cgggccgcaa ggagcgcggc ttcgttgacc aggtttgcaa
    18061 ggtcggcgcc ggtgaagccc ggagtccccc tagcgagcgt gccgatctct acgtctgggc
    18121 ccaggggctt gcctcttgtg tgtaccctga gaatcttctc tctaccgggc agatccggac
    18181 ggtccacgac tatctgcctg tcgaaacgcc ctggcctgag gagcgcgggg tcgaggatgt
    18241 ccgcgcggtt ggtggcggcg agcatgatga tcccgctctt cgaatcgaag ccgtccatct
    18301 ccacgaggag ctggttcagt gtctgctc
//
LOCUS       DASTKM010000106        16431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_24524, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000106 DASTKM010000000
VERSION     DASTKM010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16431
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24524"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1241
                     /locus_tag="VFQ10_04465"
     CDS             81..1241
                     /locus_tag="VFQ10_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HET9964625.1"
                     /translation="MAEMKQRNLEEVKDLSVEEAVEIMRQAGIVGAGGGGFPTYFKYM
                     KPQPHLIVNATESEPGYWGDKLVQREHLEEFLLLFEAMKEIFGFEQISMGVHEKDREW
                     FAAYQEHATDGLYDMRYVPDTYALGEEKTLIKHSTDVKVPLFLDTPDGKRRPGMPPDV
                     GIVVNNSETLLNVYNALFLGKPTTTTFFTVYGEEIDLKVYEAPIGASFAEILGIAGLD
                     VENSGHLSLLDGGPYLHDMAIEEIGTGDGYLRRMTNALFVIARDRQGKEYADIKTPPP
                     EEGIVSLVGEISGVHIPLGGGLLKPATPLVSEGDEVEYEQKLGEPVDEGFSIGVWASM
                     GGEISSIENDIVAIAGGAISQEEAEAEAEASKAGGGPPRGEAEPFQEVETTN"
     gene            complement(1279..2112)
                     /locus_tag="VFQ10_04470"
     CDS             complement(1279..2112)
                     /locus_tag="VFQ10_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9964626.1"
                     /translation="MPETWSLRRRLTLSGGEIAYDVLGYGPPLILVHGTPSRSYIWRD
                     VAMRLADRFTIYIFDLLGFGQSERREGLDVSISGQSRLLAEMVEAWELEAPSVAGHDI
                     GGAIALRTHLLEGVPFGCIALVDSVVLRPWITPTTRHVKAHLDVYGTMPAEIFEAIVA
                     SYLRTATHMPLDQHAFATYLEGWRGESGQRLYLQKDAQLDEEDTAEFEPLLPSINIPV
                     SIIWGEQDAWLVPSLAERLHEVIPGSDLLVLPETGHFAMEDSPKEVAAALFEFFTRCS
                     D"
     gene            complement(2112..3182)
                     /locus_tag="VFQ10_04475"
     CDS             complement(2112..3182)
                     /locus_tag="VFQ10_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9964627.1"
                     /translation="MRYRSYTPGAAQSAMDFINGWFGARGLETTRYTGAGPRGDLASL
                     VVRVGEGDPRILLHGHVDVVPGEESQFELREEGDELYGRGVYDMKGALAAMMYAIEDL
                     QKLGCEATVELLIVPDEEGEYGAKAGAEVLISNGHVGDFVITGEPTDFHIGTQAKGVL
                     DLRVTLRGKSAHGSQPWLGKNAALLAYEHYRKVTDLPFTEQRSDLFPYPSVNLARIIG
                     GDVLNRVPDRCTYDLDVRFLPHQDPEEIVRQIRSVDLPADVEILFYREPTYVTRQNPY
                     VKVLREVSARHFRGNPVGVGRHGASDIVYFQRAGVPGVEFGPVGGGHHGPNEYVVTSS
                     LETYRHMIVQFVQIVGRNGKVL"
     gene            complement(3417..4706)
                     /gene="murA"
                     /locus_tag="VFQ10_04480"
     CDS             complement(3417..4706)
                     /gene="murA"
                     /locus_tag="VFQ10_04480"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9964628.1"
                     /translation="MSERFYVEGGKQLQGEIEVSGAKNAALKFVAASLLTPGKSVLHH
                     VPRIKDMRTMLALLDELGVLTRFTGNTLEIDASTVDSYTAPYELVRQMRASLVVLGPL
                     AARFGEAEVSAPGGCNLGLRKFNFHVDGLRALGVDVELDHGFIKASAPGGLRAAEVNF
                     EYPSVGATENVMMAAVLADGVTIIENGAREPEISALAGFLNSMGARVMGAGTGRIVIE
                     GVSELYPTEWTVMPDRIEAGTFLMATAATGGDVTVTNALPEHLKMELEKLREMGVDVR
                     ATPGFPGIRIRVEDTASLSGVDVATLPFPGFATDLQAQMMVLLTQASGAGIITENVYE
                     NRLQVAEELNRLDAGIDLFGGHRALVRGPRRLSGTIVQSPDLRGGAALVMAGLVSEGT
                     TVVDGAAHILRGYEDFEEKLTALGATVAFEAGAPIHR"
     gene            complement(4860..5315)
                     /gene="atpC"
                     /locus_tag="VFQ10_04485"
     CDS             complement(4860..5315)
                     /gene="atpC"
                     /locus_tag="VFQ10_04485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET9964629.1"
                     /translation="MAEDRDQWGGGRQLFCRVITPEEIIYDGEADLVIARIADGDIGV
                     LVDHSPLVSTVKAGEVRIRENDEQRVYATSDGFFKVSENLVQVLVEEAVSPEDIDVDK
                     AENRVEEAEREISEVSEEAEDRDRVLEEIRRRQRTGENFVRVARKYRGS"
     gene            complement(5315..6922)
                     /gene="atpD"
                     /locus_tag="VFQ10_04490"
     CDS             complement(5315..6922)
                     /gene="atpD"
                     /locus_tag="VFQ10_04490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET9964630.1"
                     /translation="MTESESGQSFNRTTFGGDRGEVDNGHTGAQQEGSQDGHEERREG
                     VGTVVEVKGPVVDVRFPPEDIPEIFNAVTVPIEADGETTELTLEVQQLLGDDMVRTVA
                     MASTDGLRRGVDAQDTGQPITVPVGKSVLGRVLDVLGQPVDERGPVEDAEHYWPIHRP
                     SPRFEDLATQAEQFVTGIKVMDLLCPVRRGGKVGLFGGAGVGKTVVIQELINNIALQY
                     EGTSVFAGVGERTREGNDLYGEFEEAGILPNVGLVFGQMNEPPGARFRVALTGVTIAE
                     YFREELGQDVLFFVDNIFRFVQSGNEVSALMGRMPSEVGYQPTLGTEMGALQERITST
                     KNGSITSFQAVYVPADDFTDPAPFTVFAHLDSTVELSRSLSAQGIYPAVDPLTSSSTL
                     LDPSIVGDEHYQVAQQVQSILQRYKELQDIIAILGIDELSEEDKVIVGRARRLQRFLS
                     QPFFVAEQFTGLEGKFVELDETIRSFKEVVEGKHDELPEQAFYLVGGIDEAVKKARKT
                     SGEGESEDEEYGDEESGEEESGEDNEG"
     gene            complement(6938..7804)
                     /gene="atpG"
                     /locus_tag="VFQ10_04495"
     CDS             complement(6938..7804)
                     /gene="atpG"
                     /locus_tag="VFQ10_04495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET9964631.1"
                     /translation="MASARDFQRQINTVKNQARVFSALQTVSSVKFRKAEARVRRARP
                     YAENVEQMMRDVADSASGDLPLLTGREVRRVAVCTLTADRGLAGGFNAQVLRRTVSLR
                     NEKDPGALQVASGRKAVAFFRFRRVELVEVFTGFTDNPTYEKAREIGRALTRLFDDEE
                     ADEVYLVYNRFESVLNQRPVVKRLLPVAPEEDDEAEEENGVSYMEYVPDAETVLKQLV
                     PRYVETMVWQALLEGAAGEHGARMTAMKNATDNANDLVRDLTLQMNKARQAQITQEIL
                     EIVGGAEALSAG"
     gene            complement(7808..9445)
                     /gene="atpA"
                     /locus_tag="VFQ10_04500"
     CDS             complement(7808..9445)
                     /gene="atpA"
                     /locus_tag="VFQ10_04500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET9964632.1"
                     /translation="MGRLRPEEISSILKGAITDYENRVRTEEIGQVLEVGDGIARVHG
                     LTNAMYQEMLEFEDSRGNKIIGLALNLEEDNVGAIIAGDDTHIQEGNTVRRTEQLASV
                     PVGEGVLGRVIDALGRPMDGKGEIEAEGTLPVEIVAPGIIKRQPVKQPLQTGIKAIDS
                     MIPIGRGQRELIIGDRKTGKTSILLDTIINQADQDVKCVYVAIGQKDSTVAGVVAALE
                     EAGALEYTTVVNASAAQSATLQYLAPYAGCAIGEFFMNRGEDALLIYDDLSKHAVAYR
                     QMMLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDDAGAGSLTALPVIETKAGDISAY
                     IPTNVISITDGQIFLDSDLFNSGQRPAIDTGNSVSRVGSSAQIKAMKSVAGTLRLDLN
                     QYRELASFAQFGSDLDKATQQTLNRGERLTAILKQDERDPMRVEEQVAVLYAASNGYL
                     DDIETDAVTDWERQFRDFMRDTHGDLLDSVRSEQQLSDENEEKLREAVEQFNSNYEPE
                     HTSLVDVSVGDNEDEEDNAPEPENGEGEDQTREEEED"
     gene            complement(9445..9984)
                     /gene="atpH"
                     /locus_tag="VFQ10_04505"
     CDS             complement(9445..9984)
                     /gene="atpH"
                     /locus_tag="VFQ10_04505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET9964633.1"
                     /translation="MSAVSTYAEALFEAARERDELEEVLSDLQEFVTALRETEELRLF
                     FYGGQVPERQKRRALDGLTEGMKTSTTNFLKVLADNGREEILEDVLRLYEEIVKEHLG
                     RIEVEVTTAVELSGESERRLRERLGNLLEGREVILETNVDQDLIGGAVFRFGGRMMDG
                     SIRGRLESLREGMLERSVV"
     gene            complement(9981..10532)
                     /gene="atpF"
                     /locus_tag="VFQ10_04510"
     CDS             complement(9981..10532)
                     /gene="atpF"
                     /locus_tag="VFQ10_04510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF017242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET9964634.1"
                     /translation="MLDPTGIFDLGNTSLLFWEIITFFILLFLLVRYVYPPIRKQIQE
                     RQTQIERSIDEAEKTRAEARELLAEYRRQIEEARGEGRRILEDARKQAEAQRERTKRE
                     AREEGDRMIERAREEIDRERESALRELRREVADMVVQASEQVIGRSIDRDEHERLISQ
                     AIDDLEAEVAGATSGPGDGSGIA"
     gene            complement(10567..10779)
                     /gene="atpE"
                     /locus_tag="VFQ10_04515"
     CDS             complement(10567..10779)
                     /gene="atpE"
                     /locus_tag="VFQ10_04515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET9964635.1"
                     /translation="MIGVGIAAGTAVIGPGVGMGYLIGQTIASIHRQPEAFEQTRTLM
                     FLGIGLVEAFALYGIVFALLIAFVLG"
     gene            complement(10878..11666)
                     /gene="atpB"
                     /locus_tag="VFQ10_04520"
     CDS             complement(10878..11666)
                     /gene="atpB"
                     /locus_tag="VFQ10_04520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET9964636.1"
                     /translation="MSTIALMQVSQDELREKILHTFESGRHAWVIPLHIGPIDISINK
                     GVWFLFFGAAVTFLILFIGSRLLKDRPGAYQVIVEELYGFGRNLGGQVGEEGRKWFPY
                     TLSLFVFILVLNLIGLVPNTYPVTANITFTLVLAALTFILTQFEGFRRYGAGTYLKSF
                     APPGLPLKPIMVPFMWSVEILGEFTKPLTLGMRLYANILAGHLIILVFLGLILYFGTF
                     AAIVSVPLAVVFYAFEIFVAVLQAYIFAILTQVYIELAMFREDH"
     gene            complement(11663..12094)
                     /locus_tag="VFQ10_04525"
     CDS             complement(11663..12094)
                     /locus_tag="VFQ10_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964637.1"
                     /translation="MSGHWQLAFRSAGWMALLAAVAVAVISYLFDMDSAVAFGYGAGV
                     GLLSFISTAVTVSLLTGRSKVIGVVLGVGSFVARYGFVAIALGIPMYRDLWPTLPALG
                     GFAAVYFAETVLLVPWAIRTMIIPVAERQVDEGAERRRGTR"
     gene            complement(12091..12318)
                     /locus_tag="VFQ10_04530"
     CDS             complement(12091..12318)
                     /locus_tag="VFQ10_04530"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET9964638.1"
                     /translation="MPDTRSQRNDARGNYARFVGLGFTLILIFGLPTVVGFFLDRLAG
                     TLPLFLLIGLAGGFVGGMFYVYRVLQKLDGG"
     gene            12571..13236
                     /locus_tag="VFQ10_04535"
     CDS             12571..13236
                     /locus_tag="VFQ10_04535"
                     /inference="COORDINATES: protein motif:HMM:NF044444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9964639.1"
                     /translation="MLGSGDEVSGDRGPRRRRRPEEAERAILAAARAFLEEHPFREMT
                     VEGVMVRTGLSRPAFYAYFRDRYEVVTRLLEGIGGLLFALDWRWLSGGEGGDEGREVL
                     VDALRAGSRTFVEYGPVLRAIADAAGYDARVEQVYRHGLIERLVGAVASRISRDMEAG
                     LSPSDLDPQETARALVLMTERYLLDAFGRPERQPSRRESAAVFATLEEIWVRTLYGPG
                     GSC"
     gene            complement(13281..13769)
                     /locus_tag="VFQ10_04540"
     CDS             complement(13281..13769)
                     /locus_tag="VFQ10_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET9964640.1"
                     /translation="MEPFEDPAPDTSDSHEVLKKVRPSWNEYFMRIAHEVATRSTCPR
                     LAVGAVIVRDKRILTTGYNGSPSGMPHCEDVGCLIRIVDGRESCQRTLHAEQNAIIQA
                     AYHGVSVTGASIYCTHQPCLMCVKMIMNAGIDAVHYAGGYPDPLAIELAAEGGLAMVR
                     IE"
     gene            complement(13756..14403)
                     /gene="upp"
                     /locus_tag="VFQ10_04545"
     CDS             complement(13756..14403)
                     /gene="upp"
                     /locus_tag="VFQ10_04545"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET9964641.1"
                     /translation="MSETSPDVKNLHIVDGPLSRHKLAVLRDERTTTVGFRQAMKELA
                     LIMVAEATGNMPTRPVRIRTPLTETEVEEISGPVCLVPVLRAGLGMLDGALDLLPTAT
                     VGFMGLQRDEETAQPIEYYVNLPRNLDEYLVIVLDPMLATGGSLSATIDKLKREGATW
                     ISCLHAVAAVPGVERVSSEHPDVTFYAAAVDPELDDRSFIVPGLGDAGDRLYGTL"
     gene            complement(14396..15619)
                     /gene="glyA"
                     /locus_tag="VFQ10_04550"
     CDS             complement(14396..15619)
                     /gene="glyA"
                     /locus_tag="VFQ10_04550"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET9964642.1"
                     /translation="MIGPYTEVVDSEVQEALDGELERQRTTIELIASENFTSPAVLAA
                     QGSVLTNKYAEGYPGKRYYGGCEQVDKVENIAIERAKRLFGAEHVNVQPHSGSQANEA
                     AYAALVDPGDKVLSMDLAHGGHLTHGMKLNFSGRTYDFVHYGVGEDGYIDYDQVRDIA
                     LRERPKMILAGASAYPRIFDFERFREISDEVGAFFLTDMAHIAGLIAADVHPSPIPYS
                     EIVTTTTHKTLRGARGGMILCREEFAKKVNSRVFPGMQGGPLMHAIAGKAVALGEALQ
                     PEFEEYARKIVANCRVLAEGLLEGGLDLVSGGTDNHLILVDLKDAGITGKQLEDSLEA
                     VGITCNKNMVPNDPEPPTVTSGIRLGTAAMTTRGMGEDEMQEIAEIIVATSHGKTSGL
                     RDRVKALTEAYPLYE"
     gene            complement(15889..>16431)
                     /locus_tag="VFQ10_04555"
     CDS             complement(15889..>16431)
                     /locus_tag="VFQ10_04555"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET9964643.1"
                     /translation="AIAALKAGKVALVPTETVVGLVATEPGLPRIRAIKGREANKPIA
                     LLCASAEEAFGLAANVPPLAEHLADIYWPGPLTLVLELSSGGTIGVRVPAGRAVRGLL
                     ESYGGPLYATSANFSGELAPAALDEVDPRVLEAVDVVVSGQRGSGEASAVVDLSGGNV
                     RLVRGTSELSTQKLARFAPE"
BASE COUNT         2906 a         5254 c         5102 g         3169 t
ORIGIN      
        1 gccaccaagg ccgccgagta cgactactcg aagattgcgc caggctgggg aaccaactag
       61 atctttaggg ggcgatggtc atggccgaga tgaagcaacg caacctcgaa gaagtaaagg
      121 acctgagcgt cgaagaggcc gtggagatca tgcgccaggc cggcatagtc ggcgctggcg
      181 gcggcggttt ccctacctac ttcaagtaca tgaagcccca gccgcacctg atcgtcaacg
      241 ccaccgagag cgagccaggc tactggggcg acaagttggt gcagcgagag catctggagg
      301 agttcctcct gctcttcgag gccatgaagg agatcttcgg attcgaacag atctccatgg
      361 gcgttcacga gaaggacagg gagtggttcg ccgcttacca ggagcacgcc accgacggcc
      421 tctacgatat gcgctacgtg cccgatacgt acgccctggg cgaggagaag accctcataa
      481 agcactccac cgatgtcaag gtgcccctgt tcctggatac gcccgatggg aagcggcggc
      541 ctgggatgcc cccggacgtc ggtatcgtcg tcaacaactc ggagaccctg ctgaacgtct
      601 acaatgcgct ctttctgggc aagccaacca ccacaacgtt tttcaccgtc tacggcgagg
      661 agattgacct gaaggtctac gaggcgccga taggggcctc cttcgcggag atcctcggga
      721 tagccgggct ggacgtggag aactctggcc acctctcgct cttggacggc ggtccttacc
      781 tgcacgatat ggctatcgag gagataggga cgggcgacgg ctacttgcgg cgaatgacga
      841 acgccctctt cgtaattgcc agggacaggc aaggcaagga gtacgccgac ataaaaaccc
      901 caccccctga ggagggtatc gtctctctgg tcggagagat ctcgggcgtg cacatcccgc
      961 tgggcggcgg tctgttgaag ccggccactc cgctcgtctc cgagggcgac gaagtagagt
     1021 atgagcagaa gttaggcgag cctgtggacg agggcttctc gatcggtgtc tgggcgagca
     1081 tgggaggaga gatctcctct atagagaacg acatcgtcgc catcgccgga ggcgcgatat
     1141 cccaggagga agccgaagcc gaggcggaag cgagcaaagc aggcggcggg ccacctcgcg
     1201 gggaggccga accgtttcaa gaggtcgaaa caactaacta agaggcagcg agggtgccgt
     1261 caacggaggg cgagggcgtt agtcgctaca tcgggtaaag aactcgaaga gtgccgccgc
     1321 gacctctttt gggctatctt ccatggcgaa atgccctgtc tcgggcaaca cgaggaggtc
     1381 tgagcccggg atgacctcat gcagacgttc ggcgagagag gggacgagcc atgcgtcttg
     1441 ctcgccccag atgatgctga cgggaatgtt gatggacggg agtagaggct cgaactctgc
     1501 ggtgtcttcc tcgtcgagct gggcatcttt ctggaggtag agcctctgtc cagactcccc
     1561 cctccaccct tcgaggtagg tcgcgaaggc gtgttggtcc agcggcatgt gcgtcgctgt
     1621 gcggaggtag gaggcaacga tggcctcgaa gatctccgcg ggcatggttc cgtagacgtc
     1681 taggtgcgct tttacgtgcc tggtcgtcgg ggttatccac ggacggagga cgacgctgtc
     1741 gaccagggcg atgcatccga aaggcacgcc ttcgagcagg tgggtgcgta gggcgatcgc
     1801 gcccccgata tcgtggccgg ctaccgaggg cgcctcgagc tcccaggcct ccaccatctc
     1861 ggcgagcagt cgtgactggc ccgagataga gacgtcgagc ccttccctcc gctccgactg
     1921 gccgaacccg agcaggtcga agatgtagat ggtgaacctg tctgcgagcc tcatggcgac
     1981 gtcgcgccag atgtaggaac ggctcggcgt gccgtgaacc aggatcaggg gcggcccgta
     2041 gccgagcacg tcgtaggcga tctcgcctcc cgagagcgtg aggcgcctcc tgaggctcca
     2101 ggtctccggc atcagagtac cttgccgttg cggccgacga tctggacgaa ctggacgatc
     2161 atgtgcctgt acgtttccag agacgaggta accacatact cgttcggacc atgatgtccc
     2221 ccgccgacgg gaccgaactc gacaccaggc acccctgcac gctggaagta gacgatgtcg
     2281 ctcgcgccgt ggcggcctac gccgaccggg ttgccgcgga agtggcgcgc agagacctcg
     2341 cggagcacct tcacgtaggg gttttggcgc gtcacgtagg taggttcgcg gtagaacagg
     2401 atctcgacat cggcgggaag gtccaccgag cgtatctgac gcacgatctc ctcagggtcc
     2461 tggtgcggga ggaagcggac gtcgaggtcg taagtgcagc ggtctggcac gcggttgagt
     2521 acgtcgcctc cgatgatgcg cgccaggttc acgctcggat aagggaagag gtcgctcctc
     2581 tgttctgtga aaggcaggtc tgtgacctta cggtagtgct cgtacgcgag gagcgcggcg
     2641 ttcttgccca gccagggctg ggagccgtgg gcgctcttac cccgcagcgt aacacggagg
     2701 tcgagcactc ccttcgcctg ggtaccgata tggaagtccg tgggctcgcc cgtgatcacg
     2761 aagtccccca cgtggccgtt ggagatcaag acctcagcgc ccgctttcgc gccgtattca
     2821 ccctcttcat cggggacgat gaggagctcg actgtggctt cgcaccccag cttctgcagg
     2881 tcctcgatgg cgtacatcat ggcggcgagg gcgcccttca tgtcgtagac cccacgaccg
     2941 tagagctcgt ccccctcctc gcgcaactcg aactgcgact cctctccagg caccacgtcg
     3001 acgtgcccgt gcaggagtat ccttggatct ccttccccta cccgcaccac gagcgaagcc
     3061 aggtccccgc ggggtcccgc gcccgtatag cgcgtcgtct cgagcccgcg cgccccgaac
     3121 cagccattga taaagtccat cgcggactgc gccgcgcctg gcgtgtaaga cctgtagcgc
     3181 acgagctgcc gggcgagcgc atggacctcg gtgttctcaa ctattaccgc cagtagattc
     3241 tccgcttttc ggaagccgac aagagcagtc tatatttcag cacgccgcct tgcaggcggc
     3301 ttgtcagcac gcgccctttc gggcgctttc agcatatcag cacttcagct ttgcgcatgc
     3361 gaaggcttta gcctgagcgt gctgactcgc tgacatgatg aggctgcagg ccgatactag
     3421 cggtggatcg gggctcctgc ctcgaaggcg acggtcgcgc cgagtgctgt gagcttctct
     3481 tcgaaatcct cgtagccgcg caggatgtgc gcggcgccat ccacgacggt ggtgccctcg
     3541 gagaccaggc cagccatcac cagcgccgcg cctccgcgca ggtcggggga ctgcacgatg
     3601 gtccccgaga gccggcgcgg gccgcggacg agtgccctgt ggccaccgaa aaggtctata
     3661 cccgcgtcga ggcggttcaa ctcctcggcc acctgaagcc tgttctcgta gacgttctcc
     3721 gtgatgatgc cagcacccga agcctgggtg agcaagacca tcatctgggc ctgaaggtcg
     3781 gtcgcgaagc ctgggaaagg aagcgttgcg acgtctaccc cggagaggga agcggtatcc
     3841 tcgaccctaa ttctgatgcc cgggaaacca ggggtggcgc gtacatccac gcccatctcc
     3901 cgcagcttct cgagctccat cttcaggtgt tcggggaggg cgttggttac cgtgacgtcc
     3961 ccgccggtcg ccgccgtggc catcaagaaa gtacccgcct cgatacggtc aggcatcacc
     4021 gtccactcgg tggggtacag ttcactgacg ccttcgatca cgatgcggcc cgtgcccgct
     4081 cccatgacgc gcgcgcccat cgaattgagg aagccggcga gggcggagat ctccggctct
     4141 ctcgcgccgt tctcgataat ggtgacccca tccgcgagca cggccgccat catcacgttc
     4201 tctgtggcgc cgacgctagg gtactcgaag ttgacctcag cggcgcgcaa gccgcctggc
     4261 gcgctagcct ttataaagcc gtggtcgagt tcgacgtcaa cgccgagagc ccgcaggccg
     4321 tcgacgtgaa agttgaactt cctcaagccc aggttacacc caccaggcgc cgagacctcg
     4381 gcctccccga agcgcgccgc gaggggcccg aggacgacta gactagcccg catctggcgc
     4441 accagctcgt agggggccgt gtacgagtct acggtggagg catcgatctc cagggtgttt
     4501 cccgtaaagc gcgtaaggac accgagctcg tcgaggaggg cgagcatggt gcgcatgtct
     4561 ttgatgcggg ggacgtggtg gaggaccgac ttccccggcg tgaggagcga cgccgcgacg
     4621 aacttcaggg ctgcgttctt ggcccctgag acctcgatct ccccctgcag ttgtttcccg
     4681 ccctcgacgt agaatctctc agacacggat cctccccggt atgccttccc ggtccgctca
     4741 tccggactcg tagtatcagt atgccaatga aaggttacga aacgacaaca gccggataac
     4801 gtctgcgtta ctaccggcga tgccgtttcg ccaggcgtgt attctagcgc ttttgggccc
     4861 tagctgcctc tgtacttgcg cgccacgcgt acgaagttct cacccgtacg ctggcgacgc
     4921 cttatctcct cgagcaccct atcacgatcc tcggcctcct ccgagacctc ggagatctcc
     4981 ctttctgcct cctcgacacg gttctccgcc ttatcaacgt cgatgtcctc aggagagaca
     5041 gcctcctcga cgagcacctg cacaaggttc tcggagacct tgaagaaccc gtcggaggtc
     5101 gcgtagactc tctgttcgtc gttctccctt atcctgacct ccccagcctt cacagtggag
     5161 accagcggag agtggtcgac cagcaccccg atatccccgt cggcgatacg ggcgatcacc
     5221 aggtcggcct caccgtcgta gattatctcc tcaggcgtga tgacccggca aaagagctgc
     5281 cgtcccccgc cccactggtc gcgatcctca gccattaacc ctcgttgtct tcgccggact
     5341 cctcttcccc tgattcctca tcgccgtatt cctcgtcctc tgactcccct tcgccgctgg
     5401 tctttcttgc cttctttacg gcctcatcta taccgccgac gaggtagaac gcctgctcgg
     5461 gcagttcgtc gtgcttgcct tcgacgacct ccttgaagga gcggatcgtc tcgtcgagct
     5521 cgacgaactt gccttcgagg ccggtgaact gctcagcgac gaagaaaggc tgtgagagga
     5581 agcgctggag gcgcctggcc cggccgacga tgaccttgtc ctcctccgag agctcgtcta
     5641 tgccgagaat ggcgatgatg tcctgcagct ctttgtatcg ctggaggata ctctggacct
     5701 gctgtgccac ctggtagtgc tcgtcgccca cgatggacgg gtccagcaac gtcgaggacg
     5761 acgtcagggg gtcgacggcc gggtatatgc cctgcgctga gagcgaccgc gagagctcga
     5821 ctgtcgagtc gaggtgggcg aacaccgtga aaggtgccgg gtcggtgaag tcgtcagccg
     5881 ggacgtacac cgcctggaag ctggtgatcg aaccgttctt cgtcgaggtt atgcgttcct
     5941 ggagcgcgcc catctcggtg cctaaagtgg gctggtagcc gacctctgag ggcatgcggc
     6001 ccataagcgc ggagacctcg ttgcctgact ggacgaagcg gaagatgttg tcgacgaaga
     6061 agaggacgtc ctggccgagc tcctcgcgga agtactcggc gatggtgacg cccgttagcg
     6121 cgacgcggaa gcgggcgcct ggcggctcgt tcatctggcc gaagacgagc cccacgttag
     6181 ggaggatccc ggcttcctcg aactcgccgt agaggtcgtt cccctcgcgc gtgcgctcgc
     6241 cgacgccggc gaatacggaa gtgccctcgt actgcagggc tatgttgttg atcagctcct
     6301 ggatgacgac cgtcttgccg acgcctgcgc cgccgaagag gccgaccttg ccgcccctgc
     6361 gcaccgggca aagaaggtcc atgaccttga tgcccgtgac gaactgctcg gcctgcgtcg
     6421 cgaggtcctc gaacctcggg gaaggacggt gtatcggcca atagtgctcc gcgtcctcca
     6481 caggcccccg ctcgtccacc ggctgcccca gcacgtcgag cacgcgcccc agcacgctct
     6541 tgcctaccgg cacggttatc ggctggcctg tatcctgcgc gtccacgccg cgcctcaggc
     6601 cgtcggtcga ggccatcgct accgtgcgaa ccatgtcgtc cccgaggagc tgctgcacct
     6661 ccagggtcaa ctcggtcgtc tcgccgtcag cctcgatagg aaccgtgacg gcgttgaaga
     6721 tctccggtat gtcctcaggc gggaacctga cgtccacgac cggccccttg acttcgacga
     6781 ccgtgcctac gccttcgcgg cgttcttcgt ggccgtcctg gctgccctcc tgctgcgcac
     6841 cggtgtggcc gttatcgacc tcgcccctgt cgccgccgaa ggtcgtgcga ttaaaggact
     6901 gtccgctctc gctctcggtc atgaaaactc cttctcttta gcccgcggag agggcctccg
     6961 cgccgccgac gatctcaagg atctcctggg tgatctgggc ctggcgcgcc ttgttcatct
     7021 ggagggtaag gtcgcgcacc aggtcgtttg cgttgtccgt ggcgttcttc attgccgtca
     7081 tgcgggcgcc gtgctcgccc gccgctcctt cgaggagggc ctgccacacc atcgtctcca
     7141 cgtagcgcgg cacgagctgc ttcaggactg tctcggcgtc ggggacatat tccatgtagg
     7201 agacaccatt ctcttcttcc gcttcgtcat cctcctccgg ggcgaccggc aggagtcgtt
     7261 tgaccacggg cctctggttc aggacgctct cgaagcggtt gtagaccagg tacacttcgt
     7321 cggcctcttc atcgtcgaag agccgcgtga gggcacgccc tatctcacgt gccttctcat
     7381 aggtcgggtt gtcggtgaaa ccggtaaaga cttcgacgag ctcaacacgc ctgaaccgga
     7441 agaacgcgac ggccttgcgg ccgctcgcga cctgcagagc gcctgggtcc ttctcgtttc
     7501 tgaggctaac ggttcggcga agcacctgcg cgttgaaccc gccggccagg ccccggtcgg
     7561 ctgtgagggt gcagacggcg acgcgccgaa cctcccgccc ggtcagaagc ggaaggtccc
     7621 ctgaggcgct atctgccacg tcgcgcatca tctgctccac gttttctgcg taggggcgtg
     7681 cacgcctgac cctggcctcc gccttgcgga acttcactga ggagaccgtc tgcagggcgg
     7741 agaagaccct cgcctggttc ttaacggtat tgatctgcct ctgaaagtcc ctggctgaag
     7801 ccaagcccta gtcctcctct tcctctcgag tctggtcttc gccctccccg ttctcaggct
     7861 ccggcgcgtt atcttcctcg tcctcgttgt cccctacgga gacatcgacg aggctagtat
     7921 gctcgggctc gtagttggag ttgaactgct ctacggcctc gcgcagtttc tcctcgttct
     7981 cgtccgagag ctgttgctcg gagcggacgg agtcgagcag gtcgccgtgg gtgtcgcgca
     8041 taaagtcgcg gaactggcgc tcccagtccg tgaccgcgtc ggtctcgata tcgtccaggt
     8101 agccgttcga cgcggcgtag aggacggcta cctgctcctc gacccgcatc gggtcgcgct
     8161 cgtcctgctt caggatcgcc gtcaggcgtt ccccgcggtt tagggtctgc tgcgtggcct
     8221 tgtccaggtc gctcccgaac tgggcgaagc tggcgagctc gcggtactgg ttcaggtcga
     8281 gcctgagtgt gcctgcgacg ctcttcatag ctttgatctg ggccgaagac cccacgcggc
     8341 tcacggagtt acccgtgtcg atggccgggc gctggcccga gttgaagagg tctgagtcga
     8401 ggaagatctg gccgtccgtg atggagatga cgttcgtcgg gatgtacgcc gagatgtccc
     8461 ccgccttggt ctcgatgacc ggcagcgccg tgagcgaccc ggctccggcg tcgtccgaga
     8521 gctttgcagc gcgctccagc aggcgagagt gcaggtagaa aacgtccccc ggatacgcct
     8581 cgcgcccagg cggtcgcctc aggagcagca tcatctgcct gtaggcgacg gcgtgcttgg
     8641 agaggtcgtc gtagatgaga agtgcatcct cgccccggtt cataaagaac tcgccgatgg
     8701 cgcaccccgc gtaaggggcc agatactgca gcgtcgcaga ctgggccgcc gaggcgttca
     8761 cgacggtcgt gtactccagc gcgccggcct cctccagtgc ggcgacgacg cctgccacgg
     8821 tggagtcctt ctggccgatg gcgacgtaga cgcacttcac gtcctggtcg gcctggttga
     8881 tgatggtgtc gagcaggatc gaggtcttac ccgtcttcct gtcgccgatg ataagctccc
     8941 gctgccctcg cccgatcggg atcatggagt ctatagcctt gatacccgtc tggaggggct
     9001 gtttgaccgg ctggcgcttg atgatgccag gggccacgat ctccacagga agggtcccct
     9061 cggcctcgat ctcaccctta ccgtccatcg ggcgtccaag ggcgtctatg acgcggccta
     9121 gcacgccctc gcccaccggc acgctcgcaa gctgctcggt gcgccttacg gtgttgccct
     9181 cctggatgtg ggtgtcgtcg cccgcgatga tggcgccgac gttatcctcc tccaggttga
     9241 gcgcgagccc gatgatcttg tttccgcggg agtcctcgaa ctccagcatc tcctgataca
     9301 tcgcgttcgt gaggccgtgt acgcgcgcga tgccgtcccc gacctcgagg acctgcccga
     9361 tctcttcggt tcggacgcgg ttctcgtagt ccgtaatagc gccttttagt atggaggaga
     9421 tctcctccgg ccttagccta cccactaaac aacactcctc tcgagcatcc cctcgcggag
     9481 gctctccaac cgcccccgga tactgccgtc catcatgcgt cccccgaacc ggaagaccgc
     9541 gcccccgata aggtcctggt ccacgttagt ctccaggatc acctcgcggc cttcgagcag
     9601 gttgcccagc cgctccctga gacgacgctc cgactccccc gagagctcca cggccgtcgt
     9661 gacctccacc tcgatcctgc caagatgctc tttgacgatc tcctcgtaga gccggagcac
     9721 atcctccagg atctcctccc gcccgttgtc cgcgagcacc ttcaggaagt tggttgtcga
     9781 ggttttcatg ccttcggtca ggccgtccag ggccctgcgc ttctggcgct cggggacctg
     9841 cccaccgtaa aagaagagtc tgagctcctc ggtctcccgc agggccgtaa cgaactcctg
     9901 gagatccgag aggacctcct cgagctcgtc tctctcacgc gccgcctcga agagggcctc
     9961 ggcgtacgtg gatacggcgc tcaagcgatc ccgctcccgt cacccgggcc cgaggttgca
    10021 ccggccacct cggcttcgag atcgtcgatg gcctgggaga tcaggcgctc gtgctcgtca
    10081 cggtctatgg agcgtcctat gacctgctct gaggcctgga ccaccatgtc tgcgacctcg
    10141 cggcggagct cgcgcaaagc cgactcccgc tcgcggtcta tctcctcgcg ggcgcgctcg
    10201 atcatgcggt cgccctcctc gcgggcctcc ctcttggtac gctcgcgctg cgcttcggcc
    10261 tgcttgcggg cgtcctcgag gatacgccgg ccctcaccgc gggcctcctc gatctggcgc
    10321 ctgtactcgg ccagaagctc gcgggcctcg gcgcgcgtct tctcagcctc gtctattgag
    10381 cgctcgatct gggtctgccg ttcctgtatc tgcttccgga tcggcgggta gacgtaccga
    10441 acgagcagga acagcaggat aaagaaagta atgatctccc agaagagcaa actggtgttg
    10501 cccagatcga agatccctgt cggatctagc ataagctccc ctcttgtttc gtcctcttaa
    10561 cctcgtctag ccgagaacga aggctatgag gagagcgaac acgatcccgt agagggcgaa
    10621 cgcctcgacc agaccgatgc cgagaaacat cagagtcctc gtctgttcga acgcctccgg
    10681 ctggcgatga atagacgcga tggtctgccc gatgagatat cccatcccaa cgcccgggcc
    10741 gatgaccgcc gtgccggccg cgatgccgac cccgatcatg gtaacgtctc cggtcgcctg
    10801 aagtagcgcc aacatgggtt ctacaagcgc caatgcctcc atctacctgc tcctttctgt
    10861 tttcttgctt gcgatctcta gtgatcctcg cggaacatgg ccaactcgat gtaaacctgc
    10921 gtgagtatag cgaaaatgta agcctgcaat accgcgacga agatctcgaa agcgtagaac
    10981 accacagcca ggggcacgga gacgatcgcc gcgaacgtcc cgaaatacag gatcaagccg
    11041 aggaacacca gaatgatgag atgccctgcc aggatgttgg cgtagagccg catccccagc
    11101 gtaaggggct tggtgaactc gccgagaatc tcgacggacc acatgaacgg aaccatgatt
    11161 ggttttaacg gtaagcctgg aggcgcgaag ctcttgaggt acgtccctgc accgtagcgc
    11221 ctgaaaccct cgaactgtgt gaggataaac gtcagggccg ccaggaccag ggtgaacgtg
    11281 atgttggccg tcaccgggta ggtgttcggt accagcccga tcaggttgag caccaggatg
    11341 aacacgaaga gcgagagcgt atacggaaac cacttccttc cctcctcccc gacctgtccg
    11401 ccgaggttgc gcccgaaacc gtagagctcc tcgacgatca cctggtaggc gccggggcga
    11461 tctttgagca accggctacc aatgaacagg ataagaaacg tcaccgccgc cccgaagaac
    11521 aggaaccaca cacccttgtt gatagagatg tctataggcc ctatgtgcag agggatgacc
    11581 caagcatgcc tgccactctc gaaggtgtgc aagatcttct ctcgcaactc gtcctggctc
    11641 acctgcatga gcgcaatcgt actcatcgag tgcccctcct ccgttctgct ccctcgtcca
    11701 cctgacgctc cgcgaccgga ataatcatcg tcctgatcgc ccacggcaca agcaacacgg
    11761 tctccgcgaa atatactgcg gcgaatccac ctaaagccgg aagagtgggc cacaagtccc
    11821 tgtacatagg tatccccagc gcaatcgcga cgaacccgta tcgcgccacg aaagacccca
    11881 ccccgagcac gacgccgatc accttcgacc gtcccgtcaa caacgacacc gtcacagccg
    11941 tcgagatgaa gctcaaaaga cccactccgg ccccgtaacc aaaggcgacg gcggagtcca
    12001 tatcgaataa atacgagatc acagccaccg cgacagccgc cagcagggcc atccagcccg
    12061 cgctccggaa ggccagctgc cagtgaccgc tcatccaccg tccagtttct ggagcacccg
    12121 gtacacgtag aacatgcctc cgacgaaccc ccccgccagc ccgatcaaca ggaacagcgg
    12181 cagcgtgccc gcaagcctgt ccagaaagaa accaaccacg gtaggaaggc caaagatcaa
    12241 gatgagcgta aacccgagac cgacgaaacg cgcgtagttg ccacgcgcgt cgttccgttg
    12301 agatctggtg tcgggcatca cccgaactat accttacggg ccaaattgtt gcaatcagtg
    12361 tcaatctccg gaaaatgtcc tttcctgcga acctccgcag gacgcccgag gcgggctctc
    12421 gccgcttcag ccgaacctcc ctcgcctcgc taccgtccac ggatctctcc tctcgtggct
    12481 ctctatcaaa atgatatctc gctattgaca tgacgtcaat agacacggtg tcaataaagc
    12541 ggtacattgt ctctcattgg aggagagagt ttgctaggga gtggtgatga ggtgagcggg
    12601 gaccggggtc cgcgcaggcg acgcaggcct gaggaggcgg agcgggcgat acttgctgcg
    12661 gcgcgggcgt ttctggagga gcacccattc agggagatga ccgtggaggg ggtaatggtc
    12721 aggaccgggc tgtcgcgacc tgccttctac gcgtatttcc gggaccgcta cgaggtggtg
    12781 acgcggctgc tggagggaat cgggggtctg ctcttcgcgc ttgactggcg ctggctctct
    12841 ggtggtgagg ggggggatga agggagggaa gttctggtcg acgcgttgcg ggctggttcg
    12901 cggaccttcg tcgagtacgg gcccgttttg cgggcgatcg ccgatgcggc gggctatgat
    12961 gccagggtcg aacaggttta caggcacggt ctgatcgaac gtctggtcgg tgcggtggcg
    13021 agccgcatct cgcgcgacat ggaggctggc ctgtcccctt ccgacctcga cccgcaggag
    13081 acggctcggg cgctggtgct catgaccgag cgttacttgc ttgacgcctt cgggcgccct
    13141 gagcgccagc catcgcgccg ggagagcgcg gcggtgttcg ctacgctgga ggagatctgg
    13201 gtcaggacac tatatgggcc tggtggttcg tgctgagggc ggttcgcgaa ccgcccctac
    13261 ggccatgtgc gggtacggcc ttactcgatc cggaccatgg cgaggccgcc ttcggctgcg
    13321 agctctatag cgagagggtc ggggtagccg cctgcgtagt gcactgcgtc gataccggcg
    13381 ttcatgatca tcttcacgca catgaggcat ggctgatggg tgcagtaaat ggaggcgccg
    13441 gtcacggaga ccccgtggta ggcggcctgg atgatggcgt tctgctccgc gtggagcgta
    13501 cgctggcagc tctcgcggcc gtccacgatc ctgatcaggc atcccacatc ctcgcagtgg
    13561 ggcatccctg agggactacc gttgtagccg gtggtcagga tgcgtttgtc acggacgatc
    13621 acggccccga cggccagccg tgggcaagtc gagcgggtgg cgacctcgtg cgcaatgcgc
    13681 atgaagtact cgttccagga aggacggact ttcttcagaa cttcgtggga gtctgaagtg
    13741 tctggggcgg gatcctcaaa gggttccata tagtctgtct ccggcgtcgc cgaggccggg
    13801 gacgatgaac gagcgatcat ccagttccgg gtcgacggct gcggcgtaga aggtcacgtc
    13861 ggggtgctcg ctggaaacgc gctctacgcc tggcaccgcg gcgaccgcgt ggaggcagga
    13921 gatccaggtc gccccctctc tcttgagctt gtcaatggtg gccgagagac tccccccagt
    13981 ggcgagcatc gggtcgagga cgatgaccag gtactcgtcc aggttacgcg ggaggttgac
    14041 gtagtactca ataggctggg ccgtctcctc gtcgcgctgg aggcccataa agccgaccgt
    14101 cgccgtcggc aggaggtcga gcgctccgtc gagcataccg aggccggcgc gcaggacagg
    14161 aacgaggcag acgggaccgg agatctcctc gacctcagtc tcggtcaggg gcgtgcggat
    14221 ccggaccgga cgggtaggca tgtttcctgt ggcctcggcg accatgataa gggcgagctc
    14281 cttcatggcc tgcctgaagc cgacggtcgt ggtccgctcg tcgcgcagga ctgcgagctt
    14341 gtggcgactc agtggcccgt ctacgatgtg gaggttcttc acatcggggc ttgtctcact
    14401 catacagcgg ataggcctcc gtaagcgcct tcaccctatc gcgcaggccg gaggtcttgc
    14461 catgtgaggt tgcgacgatg atctccgcga tctcctgcat ctcgtcctca cccatgcccc
    14521 tggtcgtcat cgccgctgtg cctagcctga taccgctcgt cacggtgggc ggctctgggt
    14581 cgttcgggac catgttcttg ttgcaggtga tgcccacggc ctccagcgaa tcttccagtt
    14641 gtttgcccgt gatcccagca tctttcaggt cgaccaggat caggtggttg tctgtcccgc
    14701 cggagacgag atccaggccc ccttccaata gcccctcggc cagaaccctg caattagcga
    14761 cgatcttgcg ggcgtactcc tcgaactcgg gctgcagcgc ttctccgagg gcgactgcct
    14821 tgccggcgat cgcgtgcatc agcgggccac cctgcatgcc cgggaagacg cggctgttga
    14881 ccttcttggc gaactcttcg cggcagagga tcatacctcc gcgggccccc cgcagcgtct
    14941 tgtgggtcgt tgtggttacg atctcggagt agggtatggg cgagggatgg acgtctgcgg
    15001 cgatgaggcc cgcgatgtgg gccatgtccg taaggaagaa ggcccctacc tcgtcggaga
    15061 tctcacggaa cctctcgaag tcgaagatgc gcgggtaggc gctggctccg gccaggatca
    15121 tcttcggcct ctcgcggagg gcgatgtcgc ggacctgatc gtagtcgatg tagccgtcct
    15181 caccgacgcc gtagtggacg aagtcgtagg tcctgcctga gaagttgagc ttcatcccgt
    15241 gcgtgaggtg cccgccgtgg gctaggtcca tggataggac cttgtcgcct gggtccacga
    15301 gcgccgcata ggcagcctcg ttggcctggc tccccgagtg gggctgcacg ttcacgtgct
    15361 cggccccgaa gagcctcttg gcccgctcga tggcgatgtt ttcgaccttg tccacctgct
    15421 cgcagccgcc gtagtagcgc ttgcctggat agccttcggc gtacttgttc gtaaggaccg
    15481 agccctgcgc cgccagtacc gcaggcgagg tgaagttctc gctcgcgata agctcgatgg
    15541 tcgtacgctg gcgttccagt tcgccgtcga gcgcctcctg gacttcggaa tccacgacct
    15601 cggtgtacgg tccgatcaag atctacctcc gcttcgggtt agagaattgc ccggcgtccc
    15661 gccgggagaa atggtggttg agcgatcctg tggggcttcg atctccccgc gtcctcccta
    15721 ccagccctgc aacggtacgg agtatagacc ttctgcgcga agccctcaaa cgggaggtga
    15781 caggtatcag cacgatccag ccttcggctc gtcagctctt cacctggttg ttttcgctct
    15841 cgcggcaggg caatatctct gtagactgct ttagggggct ggcgagcctc attcgggagc
    15901 gaaccgggcc agcttctgtg tggacagctc gctggtccca cggacgagcc gcacgttgcc
    15961 tcctgagagg tcgacgaccg ccgaggcttc accgctccct ctttgtccac tgacgaccac
    16021 gtccacggcc tcgagcacgc gcgggtctac ctcgtcgagc gctgcaggag caagttcgcc
    16081 ggagaagttc gcgctagtgg cgtagagtgg gccgccgtag gactcgagca ggccccgcac
    16141 ggccctgccg gcaggcaccc ttacgccgat ggttccgccg gaggagagct ccagtaccag
    16201 cgtgagagga ccgggccagt agatgtccgc gaggtgctcc gcgagcgggg gcacgttcgc
    16261 cgccaatccg aatgcctcct ctgccgaggc acagagaagg gctatgggct tgttcgcttc
    16321 cctgcctttg atcgcacgaa tcctcggtaa gcccggctcc gtagcgacga gaccgactac
    16381 cgtctccgta gggacgagag caaccttccc ggctttcagg gcggcgatgg c
//
LOCUS       DASTKM010000107         3314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1258796, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000107 DASTKM010000000
VERSION     DASTKM010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258796"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFQ10_04560"
     CDS             <1..213
                     /locus_tag="VFQ10_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964644.1"
                     /translation="MIEKSGAKAVSLGYDRIRFIGPIFIGDTVTVTYTISEIDEENLR
                     TRADIEVANQRGEVCTVAQHILKFLA"
     gene            225..500
                     /locus_tag="VFQ10_04565"
     CDS             225..500
                     /locus_tag="VFQ10_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET9964645.1"
                     /translation="MLSRLRLERLTPRTITDTEELREELLLTRERGYATNDEEHEEGV
                     RAVGVPVIGRPAAALSVATLTFRHSMQELEGFVPLLRKAARKVEVQL"
     gene            675..1403
                     /locus_tag="VFQ10_04570"
     CDS             675..1403
                     /locus_tag="VFQ10_04570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9964646.1"
                     /translation="MLEDKVTLITGASQGLGRALALAFAREGARVAINARSEGSIGPV
                     AEEVEDTGAEVLALAADVSKSADVERMVSETVERFGGIDVLVNNAGLLGPRVRIEEYP
                     ENEWRKVIDANLTGPFLVSKATIPHLPEGGSVINVVSGVSVEGRAEWGAYAVSKFGIE
                     GLTQILAAELAGRGIRVNAVDPGGMRTDMRAAAYPEEDPATRITPEENTAVFLYLASD
                     VSKDVTGERFKAQEFQRDWGMSGE"
     gene            complement(1518..2222)
                     /locus_tag="VFQ10_04575"
     CDS             complement(1518..2222)
                     /locus_tag="VFQ10_04575"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9964647.1"
                     /translation="MDDGLLEGQLAYYRARAGEYDEWFLRRGRHDRGPEWNRRWFLEL
                     DRVRQELARFGPTGRMLELACGTGLWTVELARHAAGITAVDASPEVLAINRARLQEAR
                     PEVPVRYVRADLFDWIPDDAYDVVFFGFWLSHVPPGRFAAFWELVRASLRPGGRAFFV
                     DSVGPEAPAEKRQLGWKPVDHTMLRRLNDGQEFRIVKIFYDPVDLEARLADMGWRFSV
                     HTTDRHLLYGFGESSR"
     gene            complement(2234..2890)
                     /locus_tag="VFQ10_04580"
     CDS             complement(2234..2890)
                     /locus_tag="VFQ10_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HET9964648.1"
                     /translation="MKKYNVHRSRAKNVISWVLSLLMILAGAALIASFFLAGRLDSTA
                     TNSDNPGGFNVPKLETTDGESTSSTGPSDKTLELTVPAMARIENDEVPYTAGNDEEAL
                     RTNAAIHLEGTGFPWDNEANVYIAGHRLGYPNTESWLTFWDLSDVVGGDKIYVTDANG
                     TEYTYNVFKTFVVSPSDTHVTDTLSGKNILTLQSCTLPDYGERLIVQAELTDTSEKAA
                     "
     gene            complement(2942..>3314)
                     /locus_tag="VFQ10_04585"
     CDS             complement(2942..>3314)
                     /locus_tag="VFQ10_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9964649.1"
                     /translation="GLLATSEGGGALVLDAREEGQYTGAVARGEARAGRVPGAAHLHA
                     DSLFDPQSGTFHSGEELARRLEEAGVPADRDEPVVAYCNGGVAATVPLFVLHRLGYKR
                     LANYDGSWNEWGVREDLPAER"
BASE COUNT          626 a         1005 c         1089 g          594 t
ORIGIN      
        1 atgatcgaga agtcgggggc caaggcggtc tctttaggct acgaccgcat caggttcata
       61 gggccgatct ttatcggtga tacggtcacg gttacctaca ctatctccga gatcgacgag
      121 gagaacctgc ggacgcgtgc agatatcgag gtcgccaacc agcgcggcga ggtgtgcacc
      181 gtggcccagc acatcctcaa gttcctggcg tagagatgga ggagatgctc tcacgcctcc
      241 gcctggagag gctgacccct cgtaccatca cggacacaga agagctgcgc gaagagctac
      301 tcctaaccag ggagcgcggg tacgcgacga acgatgagga gcacgaagag ggggtcaggg
      361 cggtaggcgt gcccgtgatc ggccgcccgg cggcagcgct gagcgtcgcg accctgacgt
      421 tcaggcactc gatgcaggag ctcgaagggt tcgtcccgct cttgaggaaa gccgcaagga
      481 aggtagaggt gcagttgtga tgaggtggtc tggtgacgat acagggtgac gaagttcaga
      541 aagcggagca tgcggagctg gtggggcgcg tgcgccgctt cgtcgctgag gaggttaggt
      601 agccagtcag ccagggattt ggcctacgag agccgacatg ctgacttgct gaaatgctac
      661 cgaagggaag gaacatgcta gaagacaaag tcacgctcat caccggtgcg agccagggtc
      721 tcggcagggc gctcgcgctc gcctttgcca gagagggcgc cagggtagcg atcaacgccc
      781 gcagcgaagg gtccatcggt ccggtcgccg aagaggtcga agatacgggg gcggaggtcc
      841 ttgccctcgc ggcggacgtg tcgaagagcg cggacgtcga gaggatggtc agcgagacgg
      901 tcgagcgttt cgggggtata gacgttctcg tcaacaacgc cggtctgctg ggtccgaggg
      961 tcagaataga agagtatccc gagaacgagt ggagaaaggt catagacgcc aacctcaccg
     1021 ggcctttcct ggtctcgaag gccacgatcc cacacctccc tgagggcggc tccgtgatca
     1081 acgtggtcag cggtgtcagc gtcgagggca gggccgagtg gggcgcctat gcggtgagca
     1141 agttcgggat agaggggctc acccagatcc tggccgcaga gctcgcgggg cgcggtatcc
     1201 gcgtgaacgc tgtcgacccg ggcgggatgc gcacggacat gcgtgccgcc gcctaccccg
     1261 aagaggaccc cgccacccgc ataaccccgg aagagaacac cgccgtattc ctctatctgg
     1321 cctcagacgt atcgaaagac gtaacgggag agcgtttcaa ggcgcaggag ttccagcggg
     1381 actggggcat gtctggcgaa tagcaaggta agactttgcc agcttgatcc gtttaccggg
     1441 tcaagtcatc tggcctttcg gatcagagat accacgaaca tggccatccg tcagacacgc
     1501 cctagccgac gcagcgatta cctgctggac tcgccgaaac cgtagagcag gtgtctatcc
     1561 gtcgtgtgca ccgagaagcg ccaacccatg tcggcgaggc gcgcttcgag gtcgacggga
     1621 tcgtagaaga tcttgacgat cctgaactcc tgcccgtcgt tgagccgtct gagcatagtg
     1681 tggtctactg gcttccaccc gagctgtctc ttctctgcgg gagcctccgg gcccacagaa
     1741 tctacgaaga aggcccgacc gcctggccgt agcgacgccc ggacgagctc ccagaaagcg
     1801 gcgaagcgcc ctggaggcac gtgcgagagc cagaagccga agaacaccac gtcgtaggcg
     1861 tcgtcgggta tccagtcgaa caggtcggct cggacgtagc ggacgggcac ctcgggacgg
     1921 gcctcctgca gcctggcgcg gttgatagcg agtacctctg gcgaggcgtc aacggcggtt
     1981 atgcctgccg cgtgacgcgc gagctccacc gtccacagac cggtgccgca cgcgagctcg
     2041 agcatcctgc ccgttgggcc gaaccgggcg agttcctggc ggacccgatc cagctcgaga
     2101 aaccacctgc gattccactc cggaccgcgg tcgtggcgtc ccctgcggag aaaccattcg
     2161 tcgtactccc cggcgcgtgc ccggtagtag gctagctgcc cttcgaggag tccatcgtcc
     2221 acggcgttgg atcctacgcc gctttctccg atgtgtcggt cagctcggcc tgtacgatca
     2281 gacgctcgcc gtagtcgggc agcgtgcagg actggagcgt gagtatgttc ttgcctgaca
     2341 gggtatctgt aacgtgggta tcggaagggc taacaacgaa ggtcttgaag acgttgtaag
     2401 tgtactctgt accgttagcg tcggtgacgt agatcttgtc gccgccgacg acgtcgctca
     2461 ggtcccagaa ggtcagccag ctctcggtgt tggggtagcc cagcctgtgc cccgcgatgt
     2521 agacgttggc ctcgttgtcc cagggaaagc cggtgccttc gaggtggatg gcggcgttcg
     2581 tccttagcgc ctcctcgtcg ttccccgccg tgtaaggtac ttcgtcgttc tcgatcctgg
     2641 ccatcgcggg gacggtaagc tcgagggtct tgtcgctggg gccggtgctg ctcgtgctct
     2701 cgccgtctgt ggtctctagc ttgggcacgt tgaaaccgcc agggttgtca ctgttcgtgg
     2761 cggtactgtc aaggcgtccg gccaggaaga aggaggctat aagcgcggcg ccggccaata
     2821 tcatgagcag gctcaacacc caggaaatga cgttctttgc cctcgacctg tggacgttgt
     2881 acttcttcat gccgccggag attctaacac cgaagttgct ccctgctctc tggtcgcgcg
     2941 ttcaacgctc tgcgggcaga tcctccctga caccccattc gttccacgaa ccgtcgtagt
     3001 tggccagtct cttgtaaccg agccggtgca agacaaagag cggaaccgtg gccgcgaccc
     3061 cgccgttgca gtaggcgacg acaggctcgt ctctatccgc aggcacaccc gcctcctcca
     3121 gcctccgcgc cagctcctcg ccggaatgga aggtaccgct ctggggatcg aacagactgt
     3181 cggcgtggag gtgggcggcc cccggtacac gccccgccct ggcctcccct cgcgccacgg
     3241 caccggtata ctgcccctcc tcgcgcgcat cgagaacgag agcgccgcca ccctcgctcg
     3301 tggcgaggag gccc
//
LOCUS       DASTKM010000108         5836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_201132, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000108 DASTKM010000000
VERSION     DASTKM010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201132"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..847)
                     /locus_tag="VFQ10_04590"
     CDS             complement(146..847)
                     /locus_tag="VFQ10_04590"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9964650.1"
                     /translation="MDVVRQQVTADELLLMPDDGFRYELVKGELRRMTPAGNVHGRVT
                     MNVGISLGGYVKAHDLGAVYAAETGFRLSSDPDTVRAPDVAFVSQANVEAVGEVEGFW
                     PGAPDLAVEVVSPTDSYADVEENIFDWIDAGTKMVVVTGKKTVAPVRQGFRFAWDAPK
                     LCFSVREPFVSKASAAGIVWGNVRAGEKLEVVSQMPQDGVIFSDGVEADYLAFDSGAV
                     ASISVAEEKASLVAA"
     gene            complement(905..2296)
                     /locus_tag="VFQ10_04595"
     CDS             complement(905..2296)
                     /locus_tag="VFQ10_04595"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET9964651.1"
                     /translation="MTQQETPHRVERDSMGEVKVPREALFGAQTRRALDNFPISDLRF
                     QRRFIQALGAIKLESANVNNEQGLLDDELKDVIVEAAEEVVEGRLDNQFVLDIFQTGS
                     GTSTNMNANEVISNRAIQILDGELGSKSPAHPNDHVNQGQSSNDVIPTAIHLSALVAI
                     KEDLLPGLRKLQEALDVKASEFDSVVKTGRTHLQDATPIRLGQEFRGYAGQIERGIQR
                     VDEAVEDLAEVALGGTAVGTGVNTHPDFASKVCERLSDRFGIEVRETENHFQAQSAMD
                     AAVFTSGALKTVAVSLLKISNDIRWLGAGPRANLAEIALPEVQPGSSIMPGKVNPVIA
                     ESATMVAAQVIGNDATIALAGQSGNFELNVMLPVIAYNLIQSIEILGSASANLADQCV
                     SGLQATDRGPALVEHGLMLATALAPEVGYDKAAEISKEAYKTGKTIREVARESTDLSE
                     DELDRLLDARKMT"
     gene            2378..2962
                     /locus_tag="VFQ10_04600"
     CDS             2378..2962
                     /locus_tag="VFQ10_04600"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HET9964652.1"
                     /translation="MDSLQHALKEWAVAVRALERGETALVVRKGGIREKAFAVPQMRF
                     LLLPGYEHQRPELLKEEYRGMMDGIPDLADDGPLRFSSFAEVEGAYEISEAEALSALD
                     PHHMWTPEYAESRFKWRPKKPLTILVLRTFLLPEVVELPYRDEYGGCKSWIGLQEPVS
                     VEGARAALSDEEFERLLVPALEVLGSLEPAPVGS"
     gene            2972..3574
                     /locus_tag="VFQ10_04605"
     CDS             2972..3574
                     /locus_tag="VFQ10_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9964653.1"
                     /translation="MGSGAITIVLIFLLLFVSWQTIYAANRDLARAKAWGDLVRKALP
                     FLALLLLVLSLFVVQSYGQGRTLLVWMAVSAVAIIGASFLSVSSLERSANRAFRAGDY
                     ARAVELYRELVEEKPLARNKAFLGAALGASERYEESVEASTQAIEDDPEYGLAYYNRA
                     LVLQRMGKKNRAVKDLEKAQETDLPRRFRSASRRMLEELS"
     gene            3571..4191
                     /locus_tag="VFQ10_04610"
     CDS             3571..4191
                     /locus_tag="VFQ10_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964654.1"
                     /translation="MRLLYPTFGALVILMVLTFLIGRQNRDLLRVRAYRDFFLRLIPP
                     LSTGVFIAGLPFVMNLATVENYGPVLLVYLCGAATLTALMTRAVTPDERRAGTAFRKG
                     EYEKAAELYDEICARRPLPRYYSALGASLDASGNPGAALEATDRAINLDPKLGIAHYN
                     RASALAALGEKARARGDLQKVFQVDSSRTLRRAAAEALETLEKTDR"
     gene            complement(4192..5088)
                     /locus_tag="VFQ10_04615"
     CDS             complement(4192..5088)
                     /locus_tag="VFQ10_04615"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9964655.1"
                     /translation="MAETLMQRVVEALPFLDDIAEGVQPKVQEAVDVGGTTARNVLDG
                     VWLEAPLHPALTDVPVGAWTAALVFDGFDVVTGKEPVRHAADASLVFGTLGALGAAVT
                     GLSDWRYLSGGSRRMGVAHGLLNTIGLVLSVVSLVLRATGRRNAGRLAFLTGYSISGM
                     AAHLGGELSYHYGLRVNRNVFEAAGPDEFVPVLGESELAQDDLRRVEVEGVGVLLSRS
                     SAGEVCAIAATCNHFSGPLEEGDREGDTVVCPLHNSRFDLCSGEVVNGPAVFPQSRYE
                     TRVRDGRIEVKAAAENIQKKVR"
     gene            complement(5164..>5836)
                     /locus_tag="VFQ10_04620"
     CDS             complement(5164..>5836)
                     /locus_tag="VFQ10_04620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET9964656.1"
                     /translation="RQEISGAVLGLVVALKPSLLPVLLWPLARRRWKALLAALISGGV
                     ATLLGVIVLGPGETLDYIGVLREQQVSAYWDNASLPSAAARLFTENPYAQNVATLPWM
                     VGIGFALGIIAIVLTALRVRHGPEVGLWALVAASLLTSPIAWHNYLVLLGPGILVLLA
                     RGRAATAFLLLALQSIPAQWPLIWNDRGTVAASLAMTLYLYILIAHWLALLAATREPA
                     AAKPG"
BASE COUNT         1046 a         1886 c         1777 g         1127 t
ORIGIN      
        1 tggagatcat gctgtcactc ctgctccctg ggcgaaaatt atacgcgagg ctcccacgtc
       61 ccgcagaaga gccctcgtca acatccccgg tcctctatct gctcaccagg aaacggtaat
      121 gaaccccagt tcgtttcacg accttctacg cggcgactag acttgccttc tcttcggcga
      181 cggagatgct ggcgactgcg ccggagtcga aggccaggta gtcggcctcg acgccgtcgc
      241 tgaagatcac accgtcctga ggcatctggc tgacgacctc cagcttctcg ccggccctga
      301 cgttgcccca cactattccc gccgccgacg ccttgctgac gaacggctct cggacgctga
      361 agcacagctt cggcgcgtcc caggcgaaac ggaatccctg gcggaccggc gccacggtct
      421 tcttaccggt cacgacgacc atcttcgtac cggcgtcgat ccagtcgaag atgttctcct
      481 cgacgtccgc gtagctgtca gtgggtgaaa ccacctcgac cgccagatcc ggcgctccag
      541 gccagaaacc ctcgacctcg ccaactgctt cgacgttggc ctggctcacg aaagccacat
      601 caggcgcacg caccgtatct ggatcgctgg ataaccggaa acccgtctcg gcagcgtaca
      661 cggcgccgag gtcatgagcc ttgacgtatc cgcccagtga gatcccgacg ttcatcgtca
      721 cacgcccatg cacgtttcct gccggtgtca tccgccgcag ttctcccttt acgagctcgt
      781 agcggaagcc atcgtcgggc atgagtaaca attcatcggc ggtaacctgt tgccggacga
      841 catccatgct ctcgttctaa aaatagccga acatcagtaa cgtcacttcc gtcgggtcct
      901 ggatctatgt catcttgcgc gcgtccagca acctgtcgag ctcatcttct gagaggtctg
      961 tgctttcgcg ggcgacttcg cggatggtct tgcctgtctt gtaggcctcc ttggagatct
     1021 ctgctgcttt gtcgtagccg acctcggggg cgagggcagt ggctagcatg agaccgtgct
     1081 cgacgagcgc agggccgcga tcggtcgcct ggaggcctga gacgcactgg tctgcgaggt
     1141 tggccgaggc tgagcccaaa atctctatgc tctggatcag gttgtacgcg attacaggaa
     1201 gcatcacgtt cagctcgaaa ttaccgctct gcccggcgag ggcgattgtg gcgtcgttgc
     1261 ctatgacctg ggcggcgacc atggtggcgc tctctgcgat cacggggttc accttgcctg
     1321 gcatgataga tgagccaggc tgcacttccg gcaatgctat ctcggcgagg ttggcgcggg
     1381 gtcccgcccc gagccagcgg atgtcgttgg agatcttgag caggctaacg gcgacggtct
     1441 tgagggcgcc gctggtgaaa acggccgcgt ccatagcact ctgggcctgg aagtggttct
     1501 ctgtctcgcg tacctcgata ccaaagcgat cggagagcct ctcgcaaact ttggaggcga
     1561 aatcggggtg ggtgttgacg ccggtgccga cggctgtgcc accgagggcc acttcggcga
     1621 ggtcttccac ggcttcgtcg acacgctgta tgccgcgttc gatctggccc gcgtagccgc
     1681 ggaactcctg ccctagacgt atcggggtcg cgtcctggag gtgcgtcctc ccggtcttta
     1741 ccacactatc gaactcgctg gcctttacgt cgagcgcctc ctggagcttc cgcaggccgg
     1801 ggagcaggtc ctcttttata gcgacgagtg cggagaggtg gatggcggtc gggatgacgt
     1861 cgttggagga ctggccctgg ttgacatggt cgttggggtg ggccgggctc ttggagccta
     1921 gctcgccatc caggatctgg atagcccggt tggagatgac ctcgttggcg ttcatgttcg
     1981 ttgaggtgcc gctccctgtc tggaagatgt cgagcacgaa ctggttgtcg agccgcccct
     2041 cgacgacctc ctccgccgcc tccacgatga cgtccttgag ttcgtcgtcg agcagcccct
     2101 gttcgttgtt cacgttcgcc gactctagct ttatggcgcc gagagcctgg ataaaccgtc
     2161 tctggaatcg caggtcgctt atggggaagt tatccagggc gcggcgggtc tgggcgccga
     2221 agagagcctc gcgggggacc ttgacctccc ccatcgagtc tcgttctacg cggtgtggcg
     2281 tttcttgctg ggtcaattgt gcctccttta tcgaagccgg aacgctctcg tcatataatc
     2341 aggcgtgcaa cggtcgcatt atacgagagg tgatgtcttg gattctctac agcacgcgct
     2401 caaggagtgg gcggtcgccg tcagggcgct ggagcgcgga gagacggcgc tcgtcgtcag
     2461 gaagggcggt ataagggaga aggccttcgc cgtgccccaa atgcgctttt tgctcctccc
     2521 cggttacgag caccagaggc cggaactact gaaagaggaa tatcgcggca tgatggacgg
     2581 gatccctgac ctcgccgacg acggacccct gcgcttctcg tcgtttgctg aggtcgaggg
     2641 ggcctacgag atctccgagg ccgaagcact gtcggcgctc gacccgcacc acatgtggac
     2701 gcccgagtac gcagagagcc gcttcaagtg gcgtccgaaa aagccgctga cgatcctcgt
     2761 cctgcgcact tttctgctcc ccgaagtggt cgagcttcct taccgggatg agtacggcgg
     2821 atgtaaatcc tggatcgggc tccaggaacc cgtctccgtt gaaggagcgc gggccgctct
     2881 ctccgatgag gaattcgaaa ggctgctcgt ccctgctctc gaggttcttg ggagcctcga
     2941 gccggctccg gtcggctctt aagggggaga tgtgggctcc ggagccataa cgatagtctt
     3001 gatcttcctg ctcctcttcg tgagctggca gacgatctac gccgccaacc gcgacctggc
     3061 aagggctaaa gcctggggag acctcgtccg caaggccctc cccttcctcg ctttgctctt
     3121 gctggtgctg agcctgttcg tagtccagag ctacggacag gggcgaacgc tcctggtctg
     3181 gatggccgtc tccgctgtcg ccataatcgg cgccagcttc ctctctgtgt cctccctgga
     3241 gcgtagcgcc aacagggcct tccgcgctgg cgattacgca agggcggtcg aactctacag
     3301 ggagctggta gaggaaaagc cgctggcccg taacaaagcg tttctggggg ccgcccttgg
     3361 ggcctctgag cgttacgagg agtccgtgga agcctcgacg caggcgatcg aagacgaccc
     3421 cgagtacggc ctcgcttact acaaccgcgc cctagtctta caaaggatgg ggaagaagaa
     3481 tagggccgtc aaggacctcg agaaggcgca ggagacggac ctcccccgtc gcttccggtc
     3541 ggcctccagg aggatgctgg aagaactctc gtgaggctcc tctatcctac gttcggcgcg
     3601 ctagtcatcc tgatggtcct cacgttcttg atcggccgtc agaacaggga cctgttgcgc
     3661 gtaagggctt acagagactt cttcttgcga ctgattccac ccctctcgac aggcgtcttt
     3721 atcgcgggtc tgccctttgt gatgaacctc gcgaccgtcg agaactatgg ccccgtcctc
     3781 ctcgtctacc tctgcggcgc cgccactctc acagccctga tgacccgcgc cgtcacccct
     3841 gacgaacgcc gtgccggaac tgccttccgc aagggcgaat acgagaaggc ggctgagctc
     3901 tacgatgaga tctgcgcccg ccgtcccctc ccccgctact actcggcctt gggggcgagc
     3961 ctcgacgcat cgggaaatcc tggcgccgca ctcgaagcca cagaccgggc cataaatctg
     4021 gatcccaagc tcggcatagc ccactacaat cgggcctcgg ccctcgcggc tctgggcgag
     4081 aaggcccgcg cccgcggcga cctgcagaag gtcttccagg tcgactcaag ccgcaccctt
     4141 cgtcgcgccg ccgcagaagc cctggagacg ctggagaaga cggacagatg actatcttac
     4201 cttcttctga atgttctcgg cggcggcctt gacctcgatt ctgccgtcac gcacccttgt
     4261 ctcgtagcga gattggggga agacggccgg cccgttgaca acctcgccgc tacagaggtc
     4321 gaagcgggag ttgtgcaggg gacagactac ggtgtcgcct tcgcgatcgc cctcttcgag
     4381 cgggccggag aagtggttgc acgtggcggc tatggcgcag acctcgccgg cagaagagcg
     4441 gctcagcaag acccccaccc cttcgacctc gacccggcgc agatcgtctt gcgccagctc
     4501 gctctcgccc aggacaggta cgaactcatc gggacctgct gcctcgaaga cgttcctgtt
     4561 gacacgcagc ccgtagtggt aggagagttc tccaccgagg tgggcggcca tgccgctgat
     4621 tgagtacccc gtcagaaagg ccaggcgccc ggcgttcctg cgccccgtag cccgcagtac
     4681 gagcgagacg acactgagga cgagcccgat cgtgttcaga agcccgtgcg ctactcccat
     4741 ccgtctcgac ccgccactca ggtaccgcca gtcggagagc ccggtcacgg ccgccccaag
     4801 cgctcccaac gtcccgaaga cgagcgaggc atcggccgcg tgtctcacgg gctccttccc
     4861 tgtaacgacg tcgaacccat cgaagacgag cgccgcggtc caggccccta ctggtacgtc
     4921 cgtgagggcc gggtgcaaag gtgcctcgag ccagactccg tcgagcacgt tacgcgccgt
     4981 cgtgccgccc acgtcgacgg cctcctgcac cttcggctga acgccctcgg cgatatcgtc
     5041 caggaagggc aatgcttcga ccacgcgctg catgagggtc tctgccatct cttcaacctt
     5101 tccttatgga tcgctaccca cttggaagta tgccacgctt tcccaggagg ctcgtatctg
     5161 ccgttatcct ggcttcgctg cagcaggctc cctcgttgcg gccaggaggg cgagccagtg
     5221 cgcgatcagg atatacagat agagcgtcat cgccagtgat gccgcgaccg tgcccctgtc
     5281 gttccagatc aggggccact gcgccgggat ggactgcaaa gcgagcagca gaaaggccgt
     5341 cgccgccctg ccccgcgcca ggagcaccaa gatgccgggt ccgagcagca ccagatagtt
     5401 gtgccaggca atgggcgagg tcaggagcga agccgcaacc agggcccaca atcccacctc
     5461 gggtccgtgc cttactctca aggcggtaag gacgatggcg atgataccaa gcgcgaaacc
     5521 gataccgacc atccaaggta acgtcgcgac gttctgggcg tagggattct ccgtgaacag
     5581 ccgtgccgcc gcggagggca gggatgcgtt gtcccagtaa gcgctgacct gttgctccct
     5641 caagaccccg atgtagtcca gcgtctcccc aggccctagt acgatgaccc caagaagcgt
     5701 ggccaccccc ccggagatga gcgcggccaa gagcgccttc cacctccgcc tcgcgagcgg
     5761 ccacaggagc acagggagaa gtgaaggctt caaggcaacg accaggccaa gaaccgcgcc
     5821 ggagatctcc tgcctg
//
LOCUS       DASTKM010000109        30201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_377005, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000109 DASTKM010000000
VERSION     DASTKM010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 30201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30201
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377005"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFQ10_04625"
     CDS             <1..302
                     /locus_tag="VFQ10_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET9964657.1"
                     /translation="EWAKGSDKLFTVELLVEALDRMHLLKDITATMSDAGVNIVSARV
                     DTIEDRTALSRFAFKAASVQHVEEVIRKIRSISDVYDVYRVSRDGTPLEHQEARR"
     gene            299..874
                     /locus_tag="VFQ10_04630"
     CDS             299..874
                     /locus_tag="VFQ10_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9964658.1"
                     /translation="MSEIEQVTLSMDGFEVNSYVVHAPEGDVVVDAGAEPEKILGATR
                     APVAAILVTHGHADHVDALEFVRRGTGAPVYMHPADAEGKIRDYDPLEDGQALDVAGL
                     GIRVLHTPGHSPGSVTFIVGHDQIVGDLILPGSVGRTDIPGASWEEIEVSIRRVMPLW
                     EEETRLYTGHGPVLMAVEELRTNPYLPPGLS"
     gene            871..2154
                     /gene="hisS"
                     /locus_tag="VFQ10_04635"
     CDS             871..2154
                     /gene="hisS"
                     /locus_tag="VFQ10_04635"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET9964659.1"
                     /translation="MTDYRGPRGTYDVFPGGGEPHTRPELWTFVEDTARELFARYNYS
                     EVRTPIFEEARLFARTAGEGSDIVVQKEMFTFLDKGEREMALRPEGTPGAVRAYIEHN
                     LYKLAQPIKLFYIGPMFRQERQQKGRYRQHTQIGVEALGTSDPLVDVEVISLLYAIHQ
                     AVGVKEEVVYVNNLGDMETRQAYVPELRAYLQRHRSELDPDSVSRMDTNPLRTFDSKH
                     EGTQAVLAEAPRITSYLSSDASAHLAAVLKGLEVLAIPYEIDDRLVRGLDYYTMTVFE
                     AKTKSGALGAQDTVGAGGRYNGLISDLGGPDLGGIGFGSSVERMLLAAASKEMTRPLD
                     VFFVTLTPEARIPAMQLAGALREEGISSDLDYGGRGMRAQFKQADRMGAYYAAILGED
                     ELALGVCTVRDMASGEERVVPVVDGAKDLIRAVAG"
     gene            complement(2180..2959)
                     /locus_tag="VFQ10_04640"
     CDS             complement(2180..2959)
                     /locus_tag="VFQ10_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9964660.1"
                     /translation="MTLPNPALEACVVVPARDEEDLIRSCLDAIATQERVSHEEYEVL
                     VILDGCTDETEERARHVGDVIPSLQLHFLDGPGQGSGPARRVGMDAACDRLLQVGRPE
                     GLIACTDADTVVAPDWLAAQLRAVSEGARAIGGRIELADDGSLPESVWRRHAEEGRLR
                     HERLLSDPNPAGETQHWQFSGASMTLTAAVYSQVGGLEPLTTLEDEHLERALRHHHIP
                     IHRLISVRVTTSPRLEGRATRGLSYDLARIALSLHEERADG"
     gene            complement(2956..4128)
                     /locus_tag="VFQ10_04645"
     CDS             complement(2956..4128)
                     /locus_tag="VFQ10_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9964661.1"
                     /translation="MNHEGHLVIVGAGPAGLATARAYREGGGRGPLTMVTTEPYPPYR
                     RPPLTKEYLRGEASREDLPIQSDGWYEENGVELRLLTSVGAIDPEHRVVITGEGEELA
                     YDVCVLATGSEPVRPPVPGGDDPEILTMRTVENSTCLQKRVGEGGRAVVVGSGFIGCE
                     AAASLALRGAAVTLASHEQSPQRTRLGHEVGQRIEGWLRDLGVNLRLGVGIGGIERTD
                     GGFAVNLDDGDALDTGTVLFATGVRPRIRLAAEAGLEVDHGIITDSSMRTSAPGIFAV
                     GDIAQAYNESAGRHLSVEHWGDALEHGRIAGTVISGSEAAWSSTPGFWSTIGDKRIKY
                     WAWGDGWDEQVFEDEGEAFTVWYGKDGVLVGVLSHGNDEDYQRGRGRIESGESFSR"
     gene            complement(4125..4700)
                     /locus_tag="VFQ10_04650"
     CDS             complement(4125..4700)
                     /locus_tag="VFQ10_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009312 - oligosaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009877 - nodulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9964662.1"
                     /translation="MKGRLGREYFEGLYAESDDPWNFETSEYERIKYGRTLEVLGERR
                     FERALEAGASIGVFTEMLAGRCDELLAVDVSERAVAAARKRLSGRKHVVVERRTLPEE
                     MPDGPFDLIVASEILYYYTREEMVATLRAFERELARGGVLLAVHWRRETRTYPLQGDE
                     VHELLLRHTRLHNTKTIVEPDYRLDLFEDPA"
     gene            complement(4697..5821)
                     /locus_tag="VFQ10_04655"
     CDS             complement(4697..5821)
                     /locus_tag="VFQ10_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964663.1"
                     /translation="MSIFSVGKEERDLPGKTHDLDAVLARISAGASGRDAHPSFPEEC
                     FLWLESTGALAIPVPEPIGSQGRRATFAEEWRVLRSVAGADGSVGRILDGHFNGVERV
                     SLLAPEPLRSRELEAIVSGELLLGVWGADPIPGEGEPARLVRDEGGARLSGVKTFCSG
                     ATGLDRALVLTRGPAQGPPLLAYVDLSEGVKVDTAWFRAAGMRASESHRVVFEGTPVL
                     AVLGEPGELVREPYFSRDAIRTAVTWAGISDTALEAALDVLAAKAGDGKPDDVVSLAA
                     GRMLSSRGTIDNWLAHAAHRADSDPVSLPEISTELRESVALSCRQILDEAARAVGSHP
                     FTVSGSLDRARRDLELFLLQHRLEPALIRRGRQAISERRP"
     gene            complement(5868..6518)
                     /locus_tag="VFQ10_04660"
     CDS             complement(5868..6518)
                     /locus_tag="VFQ10_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9964664.1"
                     /translation="MHTDRRSPVALTFDDGPDPVWTPLVLDALAKAGARATFFVVAPR
                     ATRYPSLISCMRERGHDVAFHCSKHIRHDAMTHREIEADVESGIMALGPSVRYWRTPW
                     GLITPATEEVANKHRLELVGWSADTEDWRGGTPEEMLARVSGRISPSAIVLMHDGIGP
                     GARRDGCAATVDLVRLLVALVRSHGLEPAALDELRHPLPDRNPNFQARGVEWANGV"
     gene            complement(6523..8361)
                     /gene="aspS"
                     /locus_tag="VFQ10_04665"
     CDS             complement(6523..8361)
                     /gene="aspS"
                     /locus_tag="VFQ10_04665"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET9964665.1"
                     /translation="MQLQDKSNPYRTHTAGELRKDNAGEKVRLAGWVHRRRDHGGLIF
                     IDLRDRWGITQVTFHPERDGIFTSAEQLRPEWSISVEGEVVRRPEGNENPDLPTGTIE
                     VEATGLEILNPSETPPFEIERDRPVDETLRLKYRYLDLRRERMRDKILLRHRVVKHMR
                     DYLDARGFVEIETPLLTASTPEGARDYLVPARLYPGQFYALPQSPQQFKQLLMVAGFE
                     RYFQIARALRDEDQRGDRQPEHTQLDLEMSYTTQDEILDLIEGLYSGIVEQLTQKRLL
                     TKPFPRLTYAEAMDRFGTDKPDIRFGLELQDVSQLVADSEFKVFKGAVESGGSVRGIA
                     VGGLGDLSRRELEELTAVASSGGARGLAHFLVQADGLKSPVAKFFSEKQQAALRETLG
                     AEEGDWLFFVADKNPVVFESLSRLRLHFRDRLGLADTDVLGMCWVTDFPLFEWNDDER
                     RIEPMHHMFTMPREEDLDLLDSEPLQIIGQLYDLVANGTELASGSMRIHNPDLQQKVF
                     DVIGIGPEEAHRRFGTILTAFRYGAPPHGGIAPGVDRLVMLLTDEPNIREVMAFPKTQ
                     AARDEMMDAPGPVSEEQLEELNISLRRRPESERGSDPTARPGEGKN"
     gene            8504..9547
                     /locus_tag="VFQ10_04670"
     CDS             8504..9547
                     /locus_tag="VFQ10_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964666.1"
                     /translation="MAGAGSPESCRERSIGGKGKGGAHAGRVVYLALILGCLLVFAGC
                     GGDESQAPVTPTQSPETTAPAGAPTGSAVVGAGQAESTKDEPFKLNTSQPVPPNFEEA
                     YGRRARLVVQFYKLDQDSLSYPQGLSVDRKVNTSMMRLSTKYPTIEFFSYGITNPGPV
                     GDEEQSLQPGEYGTLAAQLGVGITPFIAMLAPSGDEYVITNLFEGYVPRPVLSQALFD
                     LAAVQVEDNTSDVNVTLEQIQTTDEGGGIEYFTVQNRGTQSVNLQGFTVRVLDPETAQ
                     VTPGAPGVTIDSDIRVEPGQNVSIGRVPDIVDDEGKRVAGTFEGGTSLDLAPGDQVAL
                     LDPGGAVASTITV"
     gene            9604..10764
                     /locus_tag="VFQ10_04675"
     CDS             9604..10764
                     /locus_tag="VFQ10_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET9964667.1"
                     /translation="MTEPVYLDNAATTPLDPRVLESMLPHLGGRRGNPSSLHAFGGSA
                     REGVEDARDSVASLIGASPGEIVFTSGGTESDDLAILGLARSAGPNQRRAVASCVEHA
                     AVREAARRLQSEGFEVSWIGVDAEGLVDPDEFAASLRPETALAAVVWANNEVGTVEPV
                     PELAEICATKGIPFHADAVQAVGRVPIDVSETPVSTLALSGHKLYGPQGVGALYVRQG
                     VSLEPIIYGGGQERGLRSGTQNVAGIAGLGAAARLAREEMDHRVAHERALRDRVMAGI
                     ESLPDVFVNGHRERRLSNNVHLTVLGVEAESLVLFLDSLGYAIGSGSACSSGGHKASG
                     VLLAMGLPEREAFSVVRITVGKDNTPEEVDGFLEAFSTAVAQLREISPLYAG"
     gene            10778..12241
                     /gene="mnmA"
                     /locus_tag="VFQ10_04680"
     CDS             10778..12241
                     /gene="mnmA"
                     /locus_tag="VFQ10_04680"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET9964668.1"
                     /translation="MSDRYRPQVVDHLVRPRNAGEISDPAGRGESGDAACGDVACFTV
                     GISENVLECVRYKVYGCAACIAAGSALAELVEGKHLLDAARVSRACLEDALGGSLPAG
                     KEHALTLVLDALHKAFEDHWNRKAGEMDFDAHRGRGAGGKSVVAAMSGGVDSAVTALL
                     LKEAGYDVATVTFRLHDGERGSRSCCSPDAVLFARDTAHRMGLPHFTLNLKEIFDERV
                     MQDFVGSYSEGKTPNPCVACNAHVKFHASSFLADELGYHHVATGHYARVDEGPTLARP
                     VDQSKDQTYVLWPVPRDLLARTIFPLGEYRKTDVRAIAEERGLAVAYTPESQDICFIP
                     DGDYRSFVRKKVAAKPGDVVDHEGQVLGRHAGVVDFTVGQRRGIGVSAPTPLYVSEVR
                     PRSGQVVVGRRQDLEVREVRVRDLNWFLDPEQAASVQVRYNGSPVPCTIEESSDGAWV
                     ACLDEPVIGVAPGQSAVFYTGDGERVVGGGVVARRSS"
     gene            12316..12696
                     /locus_tag="VFQ10_04685"
     CDS             12316..12696
                     /locus_tag="VFQ10_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET9964669.1"
                     /translation="MELFVELMKGLLFAGIAVAFFVLAWMFLRLARIFSTTEQTIKDV
                     SGQVVPLLGKTHITVDNINSQLTQLDEAVGDVAGMTGELDQTTAVITRGVRGGVIKVS
                     SATAGISRGLSTFLGGEKRPEKEE"
     gene            12699..13031
                     /locus_tag="VFQ10_04690"
     CDS             12699..13031
                     /locus_tag="VFQ10_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964670.1"
                     /translation="MASWGRVVAGVVIGVAGTLYATNEDVRKNLPKAARDLPDNVRRR
                     YRSAVAAAREASARRREEILHDLEEHGGGNHAARGVKPDPDAGDAAMNATHEDTSTDK
                     PEQRQGRD"
     gene            13033..13320
                     /locus_tag="VFQ10_04695"
     CDS             13033..13320
                     /locus_tag="VFQ10_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964671.1"
                     /translation="MSKDHPQEYAYLRLPPHEELRSCVGLVLAGMAARARVGVGGLEE
                     AVEVLEGFHADDAPTRFRFSLAGDGVLAEVEEPATGRSTETSWRTVVELVS"
     gene            13317..14129
                     /locus_tag="VFQ10_04700"
     CDS             13317..14129
                     /locus_tag="VFQ10_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HET9964672.1"
                     /translation="MSRRYRRPDKARTRRLLVRYHKHDDQNARERVIQEQLPLAEFLA
                     RKFAGRGEPLDDLIQVASVGLIKAVDRFDVDRKIEFSTYATPNILGEIKRYFRDKGWA
                     MRVPRGLQELRQSAKEAIRNETVRTGRSPTLQELAETLDADVEGVAEALTLGRAYNTA
                     SLDAPVSQDEPDGDTIMDFQADDESPIEGLEDRILLQEAIEGLKDQQQEILKLRFNEG
                     KTQTEIADHIGVSQMHVSRLLRRALEDLRVELEYLEHEREISKLSPDGAHAE"
     gene            14119..16752
                     /gene="alaS"
                     /locus_tag="VFQ10_04705"
     CDS             14119..16752
                     /gene="alaS"
                     /locus_tag="VFQ10_04705"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET9964673.1"
                     /translation="MQSSEIRRRFLDFFAERGHMLYPSASLIPHNDPTVLLTTGGVQQ
                     FIPYFLGQERPPSTRAVSVQKCFRTPDIEDVGDWHHLTFFEMLGNFSFGDYFKKEAIQ
                     WAWAFLTEELGIEPERLWITIFEGDEDAPEDVEAKEYWMGVGVPEERIFGLPKSENWW
                     GPPGETGPCGPCSEIYYDHGEEFAKGDPAEDPKYGPGGDEGDPRFLEIWNLVFNQYEQ
                     LKDGSFLPLSKTGIDTGMGLERTTAAVQDVLYVYDTDLYAPIFEKAKQYTGVSLGDSE
                     TTDRALRIVADHARAMAFLIADGVRPGNQRREYVLRRIIRRAMLQAHSRLGMSPDQLA
                     GLAEVVVEYMGDFYEELRSAREDIRRIVAGEATRFVEIYESGMSLLASEIGRMRGGHL
                     PGDVAFMLHDTYGFPVEVTREVLAERGLSLDEAGFQAAMDAQRERAREAMRGYERVVA
                     AFRDQEISSRFVGYEREQVESRIVAVEPVPDAGGEVYVVLAENPFYATGGGQVADEGW
                     ITSEDGQLEVFDAIPAGDYQVLMARIERGSFQVGDAVRASINRVRRQQIEANHTATHI
                     LHWALRAVLGQDVVQAGSYVGPDRLRFDYRYAGKVTDDEVKRVQELSLQKITENQPVR
                     YFTTTLEEARNLGAMMLFGEKYADLVRVVEIDGFSRELCGGTHVRGTAEIGAFKILSN
                     RKHGADLYRMEVITGREALYYTTGATEKAEEVSEALKIEIEELPEAVQRLREEVQASR
                     EAAREQALKQGLEEVGDLVEGAQQVNGTRVVTGQVVAADVRGLRQISDDVKNRLSGPS
                     AVVLAAALGGKAVIVANFHPEVSRRVEAGEIVREVSGVLGGGGGGGATMAQAGGGNLE
                     AIPDALARVREILDQRLADPE"
     gene            16756..17166
                     /gene="ruvX"
                     /locus_tag="VFQ10_04710"
     CDS             16756..17166
                     /gene="ruvX"
                     /locus_tag="VFQ10_04710"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET9964674.1"
                     /translation="MSPADRGRVAALDLGEVRTGIAVSDPGGVIARPLDVIPSGDLSP
                     YLRRLVDEKGVYEVVVGVPKTLGGEVGFQARKVLSRLDALKREFPGVRFVEWDERFTT
                     RLAGSRGKRKGTARVDHLAAARMLQEYLGARESL"
     gene            17163..18272
                     /gene="mltG"
                     /locus_tag="VFQ10_04715"
     CDS             17163..18272
                     /gene="mltG"
                     /locus_tag="VFQ10_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET9964675.1"
                     /translation="MRRHDKSERGDREELHARLDALRRSGSRGIRRKRRSNAGPTALG
                     VLLVLGVLGAIFVIYSAATGGEDVSGESAEVQVVKGDTLSDAAAKLEEAGIIKNAFIF
                     ELQARYEGYGTEIKTGRYTFEPGQDSGEILHKLTVGQAAPTFTVTIPEGLTIEETAGT
                     VAADSSVPAPEFEEAARRTEYGYAFLDNSDVKTTEGYLFPAKYDFEKGVSARQIVDRL
                     LEQYLLVTQSLDFADAKNRLDLTENQVVTVASLVEKEAASPEERPLIASVIYNRMRKD
                     TPLQIDATIQYALKRPKANLSLDDLKVDSPYNTYENKGLPPGPICSPGRESLQAALNP
                     ANTDYLYYVLEANGEKHFFTKSYDDFLREKAKAGR"
     gene            18830..20152
                     /locus_tag="VFQ10_04720"
     CDS             18830..20152
                     /locus_tag="VFQ10_04720"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HET9964676.1"
                     /translation="MAPGYIFVSPKKGPGEEAPTQDAPLIVDASGEPIWFHPLQDAEA
                     DAFTFEVQEYKGETVLTWWEGLHTGYGQGEYVIFDHSYREITRVRAGNGYQGDHHEFL
                     ITPEDTALITIYSKVQRDLSGVGGPVDGVVLDGIVQEIDIESGEVLFEWHSLDHIGLE
                     ESLYEPPPDLNAGFDYFHINSIDPIPGGYLIISARRTCAVYKVDRKTGEVVWRLGGEQ
                     SDFDMGYGTRTDWQHDARRHSDGTITIFDNGGVQKDVQSRGIVVEVDEDDMSATLVGE
                     YTHPNKILAATQGNLQVLPNGNVFVGWGSEPSYSEFGPDGKLLYDASFPSQVESYRAF
                     RFPWKGQPEDRPAVVAEAGPDDKVTIYVSWNGATEVDTWQVLAGGNPDQLEPAGSGPR
                     KGFETAVTLYTNEPYVAVEAKDSSGRVLGTSEAVEPVEPSASTDRTRS"
     gene            20174..22363
                     /locus_tag="VFQ10_04725"
     CDS             20174..22363
                     /locus_tag="VFQ10_04725"
                     /inference="COORDINATES: protein motif:HMM:NF039646.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HET9964677.1"
                     /translation="MDKSRIQLRWFEAGVALAVIAAFAAIGPFRDTFAPIAWVMLLCT
                     LVLFLTPGVLLVRWFLRDYFHGAALAPAACVTSVGFFALLAVPMLILQSTLEAYLWAC
                     GTIVAACWLAAVVLTFRPEQAHGGHTGLALSDRGGLLWAPFAVLVAALSYVARNTAPS
                     YYGDIWIYLSWVREYLGGDRLASAEPFFGSEVGLSRARINGWLLEQAAVSRVSGVDPV
                     DLVFSYLNPALVVVAFLVFYALARILFESEKAALLSGCLYALFFLVHLSQSRITWGGE
                     FVQRLPEDKLVAKFLFLPMALAFAYAFLQGGTKRYFLGFSFLCCAVMAVHPIGLAIIG
                     ISMAGCAILHLAANPRSRAAWARISAMGLTGVAIIVVPAVLVSSFTDESLSNALADSD
                     INSGDPDVLRNMIFVSPERNRIFELADGSYIMHPSLILDPVIATAFLIGLPFLLWRLK
                     RSLAAQLLFGMMYVTTVIVYVRPIATFLGDNVVLPGQIWRLAWPVQLAAVLTLGWLVW
                     TVTDYVAAWLRSLGPARFLAGALPILLVVALTAAAVPQARQGMESIQAHREASRKAGF
                     YPSDPIFPWFRDEMRSSVVVLAPDIQCARIPAYSSEANVVSRRGGLVLRVLPELQKRA
                     PGQIEVPQGSLDVQEFFSGTTLQKAVEILRRNNVDYVMVAKDSQLTGSLQRLSGFTPV
                     ETPSERYDLYAVSLQEVASKSGPPMSSPGALSVEWPSLDTRFGRQIS"
     gene            22360..23037
                     /gene="rpe"
                     /locus_tag="VFQ10_04730"
     CDS             22360..23037
                     /gene="rpe"
                     /locus_tag="VFQ10_04730"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET9964678.1"
                     /translation="MTFFGRLGDRVIGAPSILAADFSELAEEVRRAERGGAELIHFDV
                     MDNHFVPNLTVGPAVAASLAAAVDLPIDAHLQVTNPDNLIPSFIEAGVVSISVQPEVV
                     THLHRTLDLIRTGGCEAGVALNPTTPPESIEWALPYVSYVNVMSVNPGFGGQAFIPEM
                     VEKVRHLKEKTDVPVEVDGGITVETAPLMVRAGAQVLVAGSAVFKGDPAAEMRRIIEA
                     GQRASQH"
     regulatory      23094..23269
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            23271..24389
                     /gene="ribD"
                     /locus_tag="VFQ10_04735"
     CDS             23271..24389
                     /gene="ribD"
                     /locus_tag="VFQ10_04735"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET9964679.1"
                     /translation="MLRDSMMDLARCLAERGRYSVAPNPLVGAVVARDGSLLSEGWHS
                     RAGEDHAEIVALSRAGGASRGATLYVTLEPCNHYGRTPPCVEAIMRAGISHVVVGHLD
                     PDPRMQGSSVRILREAGVEVDVLDDPAFERQNEQFLHHMQTGKPFVHLKLATTLDGRI
                     AASGGDSRWVTGKDARLRAHELRAEAGAVLVGANTVRTDDPMLTARDLPVRSPRITRV
                     VLDPHLTISQESKLAKSAGETPVVVFASEPALGGREKELERLGVEVVVIPLSEEGLDL
                     VYILDELGRRGLRGLLVEGGGETATRFVRMGLADKLTLFYAPKLLGSEGVPMIGALRA
                     TKVAESLQFSVSEVGRVGDDVAVTLYPGGEEERVHRAC"
     gene            24370..25005
                     /locus_tag="VFQ10_04740"
     CDS             24370..25005
                     /locus_tag="VFQ10_04740"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET9964680.1"
                     /translation="MFTGLVEEVGRVSSIGEDEMLRLSISVGRVSEGTRAGDSVCVNG
                     ACLTVSEVDGMTLTFFAMPETLRRTALGDLAVGSPVNLERAMSAGSRFGGHIVQGHVD
                     GVGEALGVRPEGDAEIWEFGAPGTVLRYCVEKGSICVDGISLTIVSIGDGTFTVSILP
                     QTRANTNLGELGAGSRVNLEADIIGKYVERLLEPRLVGRLSTFERSAADAF"
     gene            24995..26197
                     /locus_tag="VFQ10_04745"
     CDS             24995..26197
                     /locus_tag="VFQ10_04745"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET9964681.1"
                     /translation="MPFSPIEAIVEDIKQGKMVIVCDDEDRENEGDLTMAAELVTADD
                     INFMAVHGRGLICLPMAGEIVDRLDIPQMVTHNASRMGTAFTASIEAKEGITTGISAA
                     DRAHTCRVAVDEATGPEDIVMPGHVFPLRAKAGGVLQRAGQTEAAVDLARMAGFKPAG
                     VICEIMKEDGTMARVPDLERFSREHGVKLVTVAQIIEYRHAFETQVRCAVEARLPTPF
                     GEFRLRAYENDIDELTHVALVMGEPEGKDDVLVRVHSACLTGDAFHSLRCDCGEQLEA
                     AMSSIAAEGEGVIAYMQQEGRGIGLLNKMRAYHLQDEGMDTVEANQKLGLAPDLRDYG
                     VGAQILKDLDLKRIRLLTNNLTKVVGLRGFGLEITERVPIEMEPNGHNERYLKTKREK
                     LNHVFEKF"
     gene            26315..26812
                     /gene="ribE"
                     /locus_tag="VFQ10_04750"
     CDS             26315..26812
                     /gene="ribE"
                     /locus_tag="VFQ10_04750"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET9964682.1"
                     /translation="MEGVNHIEGNLSAEGRSFGIVASRFNDFVVKALLEGAIEAIRRH
                     GGEAGAADVVWVPGSYEIPVVARELALSGRYDALICLGAVIRGATAHFDYVAGGAAGG
                     ISNVALETGVPVIFGVITTESIEQAIERAGTKMGNKGFEAAVSAMEMADLMAKLRERA
                     SLSNP"
     gene            complement(26816..27040)
                     /gene="rpmB"
                     /locus_tag="VFQ10_04755"
     CDS             complement(26816..27040)
                     /gene="rpmB"
                     /locus_tag="VFQ10_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET9964683.1"
                     /translation="MAKVCYSCGKKPDFGNARSHSLRATRRRRDPNLQKVRIQEGSAT
                     RREWVCTSCLKRFKVKKASIGAPSSASQAV"
     gene            27212..28789
                     /locus_tag="VFQ10_04760"
     CDS             27212..28789
                     /locus_tag="VFQ10_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET9964684.1"
                     /translation="MAIEVAYMVAAAHAAVKAQVARINDLNVYPVPDGDTGTNMLLTL
                     ASALNETSGKSYGSAQEASRACARSALMGARGNSGVILSQMIRGACEVLADRRSLDAD
                     AFAAGLEGARERAYASVREPVEGTMLTVIKDAALAAREAAEGGNADLPSVVGAARQAA
                     HDSVRRTPELLAVLREAGVVDAGGLGVAVVLDGVYACISGQEIEVPQADEDDAPDLEA
                     IHAQEAAWGYCTEFLVDGFEGDVEDFKEHVYASGRSVLVVAQDDVVKVHVHTQDPGGA
                     LSYAGGFGRLAGVKVDDMEAQIRSRGPEDRPAARLGVVAASRGEGNRALFEQMGAVVI
                     EGGQGENPSAADLAGAVDETGSSAVVLLPNNKNIVPTAAQVSELVEAKTYLVPTTNLA
                     AGLAVMVGYDAEGEPDEVVEEMCEISDSLRVGEVTRSVRDARIGDREVPEGAYIGFLD
                     GDLMAVENGACQTALVLVRKIVGDGADILTLLKGEELAEAELERILDGIRELDDDLEV
                     ETRDGGQPLYPLQMVAE"
     gene            28786..29601
                     /locus_tag="VFQ10_04765"
     CDS             28786..29601
                     /locus_tag="VFQ10_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET9964685.1"
                     /translation="MTTAVVTDSTTSLAPGVLERPDVRVVPLTFHFGEDEAYTDKVDM
                     TNEEFYERLRDSDLFPTTAQPSPGTFVEAYEALSDYDDILVLTLSSKFSGTYDSALQA
                     AGMVDRPVHVLDSKSAEMGSGLILLEALRCIDEGGDFDEVTHAAVRAIGGTRVLFAVG
                     TLEYLAKSGRIGRAQRLLGTALDVRPVLEIGGGEIIPFKRTRGRRRQMATIVEEVRPA
                     AEAGRQLYFGHIAAPELLSELAEQLGVEEKLVAEIGGVVGSHVGLGAYGVAHL"
     gene            29588..>30201
                     /locus_tag="VFQ10_04770"
     CDS             29588..>30201
                     /locus_tag="VFQ10_04770"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET9964686.1"
                     /translation="MPTYRGGGAQGAPLPARTTLTDLRSRPVTELPGVGPRIEAALGD
                     LGIASLADMISHYPSRHEDLSNVKRISELRVGERATVFGTVVSTRPVGKPVRGRAPGF
                     SAQLYDGTGYIPVTVWGRSWLMNQLLPEVRVVASGEVQSRYGIQIAARSIEVVDDPGA
                     DEGPHAGRFVPVYPVNKGIQARRMRTLIHRALDTAGRILDPLPA"
BASE COUNT         5533 a         9246 c         9963 g         5459 t
ORIGIN      
        1 tcgaatgggc caaagggtct gacaagctct tcaccgtcga gctgctcgtc gaagccctcg
       61 acaggatgca tctgttgaag gacataacgg ccacgatgtc ggacgccggc gtcaacatcg
      121 tctctgccag ggtcgacacc atcgaggaca ggacggccct aagtaggttc gcgttcaagg
      181 ccgcgagcgt gcagcacgtc gaggaggtaa tacgcaagat ccggagcatc tccgacgtct
      241 acgacgtcta cagggtctcc cgcgacggca cacccctgga acaccaggag gctcgccggt
      301 gagcgagatc gaacaggtca cactctcaat ggacggcttc gaggtcaact cctacgtggt
      361 ccacgcccct gaaggcgacg tcgtcgtaga cgccggcgcc gagccggaga agatcctcgg
      421 cgccacccgc gcccctgtgg cggcgatact ggtgacgcac ggccacgccg accacgtgga
      481 cgccctggag ttcgtacgga gggggacggg agcccccgtc tacatgcacc ccgcggacgc
      541 cgaggggaag atcagagact acgaccccct cgaagacggc caggcgctcg atgtcgccgg
      601 gctcggcatc agggtccttc atacgccggg acactcccca ggctccgtga cgttcatcgt
      661 agggcacgac cagatcgttg gtgacctcat actcccaggg agtgtgggcc ggacggacat
      721 ccctggcgct tcgtgggagg agatcgaagt ctccatccgc agggtgatgc cgctctggga
      781 ggaggagacg cgcctctaca cgggccacgg tcctgtactc atggcggtgg aggagttgag
      841 aaccaatcct tatctgcctc ctggcctctc gtgacggatt accggggacc gaggggcaca
      901 tacgacgtct tccctggagg tggggaaccg cacacgcgac ccgagctgtg gaccttcgtc
      961 gaggacacgg ctcgggagct cttcgcccgc tacaactact ccgaggtccg cacgcccatc
     1021 ttcgaggagg cccggctgtt cgccaggaca gcgggggagg gctcggacat cgtagtccag
     1081 aaggagatgt tcacctttct ggacaaaggc gaacgggaga tggccctacg gcccgagggg
     1141 acgccggggg ctgtgcgcgc ttacatcgag cacaacctct acaaactggc ccagccgatc
     1201 aagcttttct acatcggtcc catgttccgc caggagcgcc agcagaaggg ccgctacagg
     1261 cagcacaccc agatcggggt cgaggccctc ggcacgtcgg acccgctagt ggacgtcgaa
     1321 gtgatctcac tcctctatgc catccaccag gccgtcggcg tgaaggagga ggtcgtctac
     1381 gtcaacaacc tcggggatat ggagacaagg caggcttacg tccccgagct ccgcgcctac
     1441 ctccagaggc accgcagcga gctcgatccc gactccgtgt cccgcatgga cacgaacccg
     1501 ctgcgcacct tcgactccaa gcatgagggc acccaggcgg ttctggctga ggctccgagg
     1561 atcacctcgt acctgagttc cgacgcgtcg gcgcacctcg cggccgtgct taaaggactc
     1621 gaggtgctcg ccatccctta cgagatcgac gacaggctcg ttcgtggcct cgactactac
     1681 actatgacgg tcttcgaggc caagaccaag agtggcgctc ttggggcgca ggataccgtc
     1741 ggtgccggcg gccggtacaa tggtctgatc tccgacctcg gaggacccga cctcggagga
     1801 atcggtttcg gttcaagcgt cgagcggatg ctccttgccg cagcctcgaa ggagatgacg
     1861 aggccgctcg acgttttctt cgtgaccctc acgccggaag cacgcatccc tgctatgcag
     1921 cttgccggag ccctgcgcga agaggggatc tcgagcgatc tggattacgg ggggcgcggc
     1981 atgagggcgc aattcaagca ggccgatcgt atgggcgcct actatgctgc catactcggt
     2041 gaggacgagc tcgctctcgg cgtctgcacg gtgcgggaca tggcttcggg cgaggagcgg
     2101 gtggtgcccg tggttgatgg ggcgaaggac ctgatccggg ccgtcgccgg ctgagtatag
     2161 ggagagagga cgtagcgccc tagccgtctg cgcgttcctc gtgaaggctc agtgctattc
     2221 tggcgaggtc gtatgaaagg cctcgtgtag ctctcccctc cagacgaggc gatgtcgtca
     2281 ccctgactga aatcagccgg tggatcggaa tgtggtggtg gcgcagagcc ctttcgagat
     2341 gttcgtcttc gagggtggtc agcggttcga ggccgccaac ctgcgagtag actgccgccg
     2401 tcagggtcat ggaggcgccg gagaactgcc agtgctgtgt ctcgccggct ggattggggt
     2461 cagagagaag gcgctcatgt cgcaggcggc cctcctccgc gtgcctgcgc cagacactct
     2521 caggcaacga gccatcgtcg gcgagttcga tgcgcccgcc gatggccctt gctccctccg
     2581 agacggccct gagttgggcg gcgagccagt cgggcgccac caccgtgtca gcatccgtgc
     2641 acgctatgag gccctcgggg cggcccacct ggagaagacg gtcgcatgca gcatccattc
     2701 cgaccctgcg ggcggggcca gatccctggc caggaccatc caggaagtgg agttgcagcg
     2761 atggaattac gtcgccgaca tggcgggcgc gttcttctgt ttcgtccgta cagccatcga
     2821 gtatcacaag aacctcgtac tcctcgtgtg agacacgctc ctgcgtcgcg atcgcatcga
     2881 ggcaagatct tatgagatcc tcctcgtcgc gggctggcac gaccacgcag gcctcgaggg
     2941 ctgggttcgg cagcgtcatc gggagaagga ctctccgctt tcgatccgcc cgcgccctcg
     3001 ttggtagtcc tcgtcgttgc cgtgggagag aactcccacg agcacaccat ctttgccgta
     3061 ccacacggtg aacgcttctc cctcgtcttc gaagacctgc tcgtcccaac cgtctcccca
     3121 ggcccagtac ttgatcctct tgtcgccgat tgtggaccag aagccggggg tactgctcca
     3181 ggcggcctct gaaccggaga tcacggtacc cgcgatgcgt ccgtgctcca gagcgtcgcc
     3241 ccagtgctcg acgctgaggt ggcgccccgc ggactcgttg tacgcttggg cgatgtctcc
     3301 gaccgcgaag atccctggcg cgctcgtccg catcgaggag tctgtgatga tcccgtggtc
     3361 cacctcgagc ccggcctccg cggcgagccg gatgcggggc ctaacacccg tcgcgaagag
     3421 gacggttccc gtatcgagag catcaccatc atcgaggttc acagcgaagc cgccatccgt
     3481 gcgttcgatg cctccgattc cgacaccgag gcgcagattg acgccgaggt cccgtagcca
     3541 accttcgatg cgttgtccga cctcgtgacc gagccgcgtc cgttgtggcg actgttcatg
     3601 gctggccagg gtcaccgcgg cgcctcgcaa cgcgagggaa gcggccgcct cacatccaat
     3661 gaatccgctc cctaccacga cggccctgcc tccctctccg acacgcttct gtaggcaggt
     3721 cgagttctcg accgtcctca tcgtcaggat ctcggggtcg tcgcctccag gcacgggcgg
     3781 ccgtactggc tctgagcccg tggccagcac gcagacatcg tacgctagct cttcaccctc
     3841 gcctgtgatg acgacccggt gctcggggtc tatcgcgccc acgctggtga ggagcctgag
     3901 ctccaccccg ttttcctcgt accagccgtc gctctggatg ggtaggtcct cgcgggacgc
     3961 ctcgcccctc aggtattctt ttgtaagcgg aggacgcctg taaggagggt atggctcggt
     4021 ggtcaccatc gtgagcgggc ccctgcctcc tccttccctg taggctcgtg ccgtggccag
     4081 gcctgcggga ccggcgccga cgatcaccag gtgtccctca tggttcacgc cgggtcctcg
     4141 aagagatcca ggcggtaatc aggctcgacg atggtcttgg tgttgtggag ccgtgtgtgc
     4201 ctcaggagta gctcgtgtac ctcgtcaccc tgcagcgggt aggttcttgt ctctctgcgc
     4261 cagtggacag cgagcaggac tcctcccctg gccagctcgc gctcgaaggc ccgtagcgtc
     4321 gcgaccatct cttccctcgt gtagtaatac aggatctccg aggcgacgat caggtcgaac
     4381 gggccgtcgg gcatctcctc tgggagcgtg cgccgttcga ccacgacgtg cttacggccc
     4441 gagagccgct tgcgggccgc ggccacggcc cgctccgaca cgtctaccgc aagtagctcg
     4501 tcgcagcggc ctgcgagcat ctctgtaaag acgccgatgg aagcacccgc ctccagggct
     4561 ctctcgaacc gcctttcgcc aaggacctct agcgttcgcc cgtatttaat gcgctcgtac
     4621 tcgctcgtct cgaagttcca gggatcatct gattccgcgt agagcccctc aaagtactcg
     4681 cgtccgagtc gccctttcac ggccgccgct ccgagattgc ttgcctcccg cggcgtatca
     4741 gggcaggctc gaggcggtgc tggaggagga agagttcgag atctcgcctc gcgcggtcca
     4801 gggagccgga gacggtgaag gggtgcgagc cgaccgctct ggccgcctcg tcgagaatct
     4861 ggcggcacga gagggccacg gactcacgca gctctgtcga gatctccgga agagagactg
     4921 ggtccgagtc ggcgcggtgt gctgcgtgcg cgagccagtt gtcgatggtg cccctggacg
     4981 agagcatcct gcctgcggcg agagagacta cgtcgtcggg tttcccatcc ccggctttgg
     5041 cagccagtac gtcgagcgcg gcttcgaggg ccgtgtcgga gatcccggcc caggttacgg
     5101 cagttcggat ggcgtctctg ctgaagtatg gttccctgac gagctcgccc ggttctccaa
     5161 gaactgcgag caccggcgtt ccttcgaaga cgacgcggtg actctccgag gcgcgcatgc
     5221 ccgcggccct gaaccacgcc gtatctacct ttacgccttc ggagaggtcg acgtatgcaa
     5281 ggaggggcgg tccctgggcg gggccgcgtg tgagcactag cgcgcgatcc aggcccgtcg
     5341 caccggaaca gaaggtcttg acaccggaga gcctagctcc gccctcgtct cgaacgagcc
     5401 gagccggttc tccctcgcct ggaatcggat cggcgcccca cacgccgagg aggagttccc
     5461 ccgagactat agcttccagc tccctcgagc gcaggggctc gggggccagg agcgagacgc
     5521 gttccacgcc gttgaagtgg ccatcgagta tgcggccgac ggagccgtcg gcaccggcga
     5581 ccgaccgcag cacgcgccac tcctccgcga acgtggccct gcgtccctga cttccgatgg
     5641 gctcgggaac gggaatagcg agcgcgcccg tagattcgag ccacagaaag cactcttccg
     5701 gaaatgaggg gtgcgcgtcc cgcccggacg cgccggcgga gatccgcgca aggaccgcgt
     5761 cgaggtcgtg ggtctttcca ggaaggtctc gttcctcttt accgacgctg aatatactca
     5821 tcgcgacatt actttaaagg agcagatgac ccgtgcaaag agaaccctca cacaccattc
     5881 gcccattcga ctcccctggc ctggaagttc gggttcctgt cgggaagcgg atgcctgagc
     5941 tcgtccaacg ccgcgggctc caggccatgg gatctcacca gggcgacgag cagtcttacg
     6001 agatccacgg tcgctgcgca gccgtctcgc ctcgctccgg ggcctattcc gtcgtgcatc
     6061 aggacgatgg cactgggcga gatcctacca ctcacgcggg cgagcatctc ctccggcgtc
     6121 cctcctcgcc agtcctccgt gtcagcggac cagccgacga gctcgagccg gtgtttgttt
     6181 gccacctctt cggtcgccgg tgtgatgagg ccccaggggg ttctccagta ccgcacggac
     6241 ggcccgagcg ccatgattcc tgactccacg tctgcttcga tctccctgtg tgtcatagcg
     6301 tcgtggcgga tatgcttgct gcaatgaaag gcgacgtcat ggcctctctc cctcatgcag
     6361 gatatgaggg aagggtaacg tgtagcccgt ggcgcgacga cgaagaacgt ggcacgtgcc
     6421 ccggccttgg ccagggcgtc caaaactagg ggagtccaga cggggtcggg tccgtcgtcg
     6481 aaggtcaggg ccaccggcga ccggcgatcg gtgtgcatta ctctagtttt tgccctcgcc
     6541 tggccgggct gtggggtcgg agcctcgttc gctttccggc cggcggcgga gcgagatgtt
     6601 tagctcctcg agttgttctt ccgagacggg accgggggcg tccatcattt cgtcgcgggc
     6661 ggcctgggtc ttggggaacg ccatcacctc acggatgttc ggctcgtccg tgaggagcat
     6721 caccaggcgg tctacccctg gtgcgatccc accgtggggc ggtgcgccgt agcggaaggc
     6781 ggtgaggatg gttccgaacc gcctgtgcgc ttcctcaggt ccgataccga tgacgtcgaa
     6841 gaccttctgc tgcaggtcgg ggttgtggat cctcatactc cccgacgcga gctcggtccc
     6901 gttggccacg aggtcgtaga gctggccgat gatctgcagt ggctcggagt cgagcaggtc
     6961 aagatcctct tcgcgcggca tagtgaacat gtggtgcatc ggctcgatgc gcctctcgtc
     7021 gtcgttccat tcgaagagcg ggaagtccgt cacccagcac attccgagca cgtcagtgtc
     7081 ggcgagcccg aggcggtctc tgaagtggag ccgcagtcgg ctcaggctct cgaagacgac
     7141 ggggttcttg tccgcgacga agaacaacca gtcgccttct tcggctccga gcgtttcgcg
     7201 cagagcagcc tgttgcttct cggagaagaa cttcgcgacg gggcttttca gcccgtccgc
     7261 ctgtaccaga aagtgggcca ggcctctagc gcctcccgag gacgcgaccg cggtgagctc
     7321 ctcgagctcc ctgcgactca ggtcccccag ccccccgacg gcgatgccgc gcaccgagcc
     7381 tcccgattcc acggccccct tgaagacctt gaattccgag tctgcgacga gctgcgagac
     7441 gtcctggagc tccaggccga agcggatgtc cggcttatcc gtaccgaagc ggtccatcgc
     7501 ctcggcgtaa gtaaggcggg ggaagggttt ggtgagtagc ctcttctgcg taagctgctc
     7561 gacgattcct gagtagaggc cttcgataag gtccaggatt tcgtcttgcg tcgtgtagct
     7621 catctccagg tcgagctgcg tatgttcggg ctggcggtcc ccgcgctggt cctcgtcgcg
     7681 cagtgcgcgg gctatctgga agtagcgttc gaagccagcc accatcagga gctgcttgaa
     7741 ttgctgtggg gactgtggga gcgcatagaa ctgccctgga tagaggcggg cagggaccag
     7801 gtagtccctt gcgccctcag gtgtggaggc cgtgaggagg ggagtttcga tctccacgaa
     7861 gccccgagcg tcgaggtagt cgcgcatgtg cttgacaacg cggtggcgga gcaagatctt
     7921 gtctcgcatc ctctccctgc gcagatcgag gtagcggtac ttcaggcgca gcgtctcgtc
     7981 aacgggcctg tcgcgttcga tctcgaaagg cggggtctcg gaggggttca ggatttcgag
     8041 cccggtagcc tcgacctcta tcgttcctgt cggcagatcg gggttctcgt tgccctcggg
     8101 acggcgcacc acctcgccct ctacggagat tgaccactcg ggcctgagtt gttcggccga
     8161 ggtgaagatc ccgtcccttt cggggtggaa tgtgacttga gtaatgcccc agcggtcgcg
     8221 caggtctatg aagatcagac ctccgtggtc cctacgcctg tgtacccagc ccgcgagcct
     8281 gactttctcc ccggcgttgt ccttgcgtag ctcacctgcg gtgtgggttc tgtaagggtt
     8341 gctcttgtcc tgaagttgca atttcgttct cctagcattt gttcgcggac tggcccacga
     8401 atgttagccg aaaacgctgt ggaccgaggc cgatggttta tccggacaca tattgcagcg
     8461 tttttcctac cgtgctaagt tagcgggaat cgaatcggag gccgtggcag gcgccggatc
     8521 tccggagagc tgtagggaga ggtctatcgg aggtaagggc aagggagggg cacacgccgg
     8581 gagagtagta tatctggcgc tcattctcgg ctgcctgtta gtgttcgcgg gatgtggagg
     8641 cgacgagagc caggcccctg taacgccaac ccagagtccc gagacgacgg ccccagcggg
     8701 cgcccctact ggcagcgccg tggtgggggc cggacaggcc gagagcacga aggacgagcc
     8761 cttcaagctc aacacgagcc aacccgtgcc gcccaacttc gaggaagcct acgggcggag
     8821 ggccaggctc gtggtccaat tctacaagct ggaccaggac tcgctctcct atccacaagg
     8881 cctctccgtg gaccgcaagg tcaacacgtc catgatgcgc ctgagcacca agtatccgac
     8941 catcgagttc ttctcctacg gcatcacgaa ccccgggccc gtcggtgacg aggagcagag
     9001 cctgcagcct ggcgagtacg gcacgctggc cgcgcagctt ggagtcggga tcacgccgtt
     9061 catcgcgatg ctcgcgccga gtggcgatga gtatgtgatc accaacctct tcgaaggata
     9121 tgtgccaagg cccgtgttga gccaggcgct cttcgatctc gcggcggtac aggtcgagga
     9181 caacaccagc gatgtcaacg tgaccctgga acagatccag acgaccgacg aaggcggcgg
     9241 aatcgaatac ttcaccgtac aaaatcgtgg gacccagtcc gtgaacctgc agggctttac
     9301 cgtgcgggtc ctcgaccccg aaaccgcgca ggtaacacct ggcgcgccag gcgtcacgat
     9361 cgacagcgat atcagagtag agccgggtca gaacgtctcc atcggccgcg ttcctgatat
     9421 tgtggacgac gagggcaagc gcgtggcagg cacgttcgaa ggaggcacgt cgctcgatct
     9481 ggctccagga gaccaggttg cgctcctcga tcctggtggc gctgtcgcct ccaccatcac
     9541 cgtctgagcg aaacgggctc tacggctgac atttcggccc attaagataa actagactcg
     9601 atcatgaccg agcctgttta cctggacaat gcagccacca cgccgctcga cccgcgggtt
     9661 ctggaatcga tgcttccgca ccttggaggc cggcgcggca atccttcttc gctgcacgca
     9721 ttcggaggct cggcgcgtga gggggtagag gatgcccgag attctgtagc ctccctgatc
     9781 ggggcttcgc caggggagat cgtgtttacc agcggaggaa ccgaatccga cgacctggca
     9841 atcctcggcc tggcgagatc cgcaggtccc aaccagcggc gcgcggtcgc ctcgtgtgtc
     9901 gagcacgcgg cggtgcggga ggccgcgaga cgcctgcaat ccgaaggttt cgaggtgtcc
     9961 tggatcgggg ttgacgctga agggctcgtc gacccagacg agtttgccgc ctcgctgcgc
    10021 cccgagaccg cgctggccgc cgtcgtatgg gcgaataacg aggtgggcac cgtcgagcct
    10081 gtgcccgagc tcgccgagat ctgcgcgacg aagggtatac ctttccacgc cgacgccgtc
    10141 caggccgtcg gcagggtccc catagacgtc tcggagacac cggtctcgac tctggctctc
    10201 tcgggtcata agctctacgg tcctcagggc gtcggcgccc tctacgtgcg tcagggcgtc
    10261 tcgctcgaac ccatcatcta cggcggaggg caggagaggg ggctgaggag cggcacccag
    10321 aacgtcgcag ggatcgccgg ccttggcgcg gctgcgaggc tggcccgcga ggagatggac
    10381 caccgcgtcg cgcacgaaag ggcgctacgc gaccgcgtca tggccggaat agagtcctta
    10441 ccagatgtgt ttgtaaacgg gcatcgcgag cggcgattgt ccaacaacgt ccacctcact
    10501 gtattgggcg tagaggctga gagcctggtc ctcttcctcg attcgctcgg ctacgctata
    10561 gggagtggtt cggcctgttc ctcgggcggg cacaaggctt caggggttct gctcgcaatg
    10621 gggttgcccg agcgagaggc gttctctgtc gtcaggatca cggtcggcaa agataacact
    10681 ccagaggagg tcgatggctt cctggaggcg ttctcgaccg cggttgcgca actacgagag
    10741 atctccccgc tctacgcggg ttgaaggcgg gttacacatg tccgatcgtt acagaccgca
    10801 ggtcgtagac cacctcgtcc ggccacgcaa cgccggtgag atatccgatc ctgctggtag
    10861 gggggagtct ggcgacgccg cgtgcggcga cgtcgcatgc tttacggtag ggatctcgga
    10921 gaacgtactg gaatgtgtcc gctacaaggt ctacggctgc gcggcatgca tcgccgccgg
    10981 ctcagccctc gctgaactgg tcgaaggcaa acacctcctc gatgccgccc gcgtttcgag
    11041 agcctgcctg gaagacgcgc ttggcggatc cctccccgcc ggcaaggagc atgcgctcac
    11101 gctcgtccta gacgcattac acaaggcctt cgaggaccac tggaaccgta aggcgggcga
    11161 gatggatttc gacgcgcaca gagggcgggg agccggcggc aaaagcgttg ttgccgcgat
    11221 gagcgggggc gttgactcgg cggtcacggc cctacttctc aaagaggcgg gctacgacgt
    11281 cgcaaccgtt acgttccggc tgcacgacgg cgagaggggc agccgctctt gctgctcgcc
    11341 cgacgcggta ctcttcgccc gcgacacggc gcacaggatg ggactgccgc acttcacgct
    11401 caacctgaaa gagatcttcg acgagcgcgt catgcaggac ttcgtgggct cctacagcga
    11461 aggcaagacg ccaaacccat gcgtagcttg caacgcccac gtcaaattcc acgcgtcgtc
    11521 gttcctcgcg gacgagctcg gataccacca cgtggcgacg gggcactacg ccagggttga
    11581 cgaaggccct acgctcgcgc ggcccgtgga ccagagcaag gaccagacct acgtcctctg
    11641 gcctgtgccg cgggatctcc ttgcgcgtac gatcttcccc ctaggcgagt accgaaagac
    11701 ggacgtccgc gccatcgccg aggagcgggg cctcgccgtc gcctacaccc cggagagcca
    11761 ggatatctgc tttatccccg atggggacta ccgcagtttt gtccgcaaga aggtggctgc
    11821 gaagccaggt gacgtcgtcg accatgaagg gcaagttctt ggccgtcacg ccggtgttgt
    11881 cgactttacg gtcggacagc ggcgcggaat aggcgtctcg gcgccgacgc cgctgtacgt
    11941 cagcgaggta agaccgcgtt ccgggcaggt agtcgtggga cgcaggcaag acctcgaggt
    12001 gcgcgaggtc agggtgaggg atctcaactg gttcctcgac cccgagcagg ccgctagcgt
    12061 acaggtccgc tacaacggct cgccggtccc ttgcacgatc gaggagtcct ctgatggagc
    12121 gtgggtagcc tgtcttgatg agccggttat aggtgttgct cctgggcagt ctgccgtgtt
    12181 ctatacaggg gacggggagc gggtcgtcgg gggcggcgtc gttgcgcgca gaagctcttg
    12241 agagcggtac cttttgcgag cgatcacagc tataatccgg gttttcaggg actctggacg
    12301 gaggtgctgt agggtttgga gttattcgta gagctgatga aggggttgct tttcgcgggg
    12361 atagccgttg ctttcttcgt cctcgcgtgg atgttcttga ggctggcgag gatcttctcg
    12421 accaccgagc agacgatcaa ggacgtctcg gggcaggtcg ttccgcttct cgggaagacg
    12481 cacatcacgg tggacaacat aaacagccag ctcacgcagc tcgacgaggc cgtaggggac
    12541 gtcgcaggca tgacgggcga gctcgaccag accacagccg tgatcacacg cggcgtaagg
    12601 ggtggtgtga tcaaggtctc ctctgcgaca gcgggtatct cgcgcgggct cagcaccttc
    12661 ctcggcggcg aaaagcgtcc tgagaaggag gagtagcggt ggcgagttgg ggtagggtcg
    12721 tggccggcgt cgtgatcggg gtggcaggca cgctctacgc gacgaacgag gacgtacgca
    12781 agaacctgcc taaagcggcg cgagatctcc ccgacaacgt ccgtcgccgc tacaggagcg
    12841 cggttgccgc ggcacgcgag gcatccgccc ggcggcggga ggagatcctg cacgatctcg
    12901 aggaacacgg cggcgggaac cacgccgcgc gcggggtgaa gcccgatccc gacgccgggg
    12961 acgcggcgat gaacgcaacg cacgaagata catcgacaga taagcccgag caacgccaag
    13021 gaagggattg acttgagcaa agatcatccc caggagtacg cttacttgag gctcccgccc
    13081 cacgaagagt tgcgttcctg tgtcggcctc gtgctggcag ggatggcggc acgggccaga
    13141 gtaggtgtgg gaggattgga ggaggccgtg gaagttctgg agggctttca cgctgatgac
    13201 gctccaaccc gctttcgctt ctccctggcc ggcgatgggg ttctcgccga ggtcgaagag
    13261 ccggccaccg ggcggagcac agagacaagc tggcgcaccg tagtcgagct ggtctcgtga
    13321 gccggcgcta tcgcagaccg gacaaagcgc gcacgcgccg cctgctcgtg cgctaccaca
    13381 agcacgacga ccagaacgca cgcgagcggg tcatccagga acaacttccc ctggccgagt
    13441 tcttggcccg caagttcgca ggccgcggtg agcccctgga cgatctgatc caagtcgcct
    13501 ccgtcggcct gataaaggcg gtggaccgct tcgatgtaga caggaagatc gagttctcca
    13561 cctacgcgac cccgaacatc ctcggtgaga tcaaacgtta cttccgtgac aagggctggg
    13621 cgatgcgggt cccccgtggc ctgcaggagc tccgccagtc ggcgaaggaa gcaatccgca
    13681 acgagaccgt gaggacgggt cggtcgccca cgctgcagga actcgccgag accctcgacg
    13741 ctgacgtgga gggtgtggcg gaggcgctga ccctcgggcg ggcttacaac acggccagcc
    13801 tcgacgcgcc cgtaagccag gatgagcccg atggggacac gatcatggac ttccaggccg
    13861 acgatgagtc cccgatagaa gggttggagg acaggatcct gctccaggag gccatcgagg
    13921 gcctaaagga tcagcagcag gagatcctga agctccgctt caacgagggc aagacacaga
    13981 ccgagatcgc agaccacata ggcgtcagcc agatgcacgt ctcccgcctg ctcaggcgcg
    14041 cactggagga cttgcgggtc gaactggagt acctggaaca tgagcgggag atcagcaagt
    14101 tgtccccgga cggggcgcat gcagagtagc gagatacgac gccggttcct ggactttttc
    14161 gctgagcggg gccacatgct ctacccgagt gcctccctca tcccccacaa cgaccctacg
    14221 gtgctcctga ccacgggcgg ggtccaacaa ttcatcccct attttctcgg ccaggagagg
    14281 ccgccttcca cgcgggcggt gagcgtgcag aagtgcttcc gtacccccga catcgaggat
    14341 gtcggggact ggcatcatct caccttcttc gagatgcttg ggaacttctc gttcggggac
    14401 tacttcaaga aggaagccat tcaatgggca tgggcgtttc tgacggaaga gctcggcatc
    14461 gagccggagc gcttgtggat aacgatcttc gagggcgacg aagacgcccc cgaggacgtg
    14521 gaggcaaagg aatattggat gggcgtcggc gtgcccgaag agaggatctt cggcctgccc
    14581 aagagcgaga actggtgggg accgccagga gagaccgggc cgtgtggccc gtgctcggag
    14641 atatactacg atcacggtga agagttcgcc aagggcgacc cggccgagga cccaaagtac
    14701 gggccaggcg gcgacgaggg agatccgcgc ttcctggaga tctggaacct cgtcttcaac
    14761 cagtacgagc aattgaaaga cggctccttc ttgcccttga gcaagactgg tatcgacacc
    14821 ggcatgggat tggagcggac caccgccgcg gtacaggatg ttctctacgt ctacgacacc
    14881 gacctctacg caccaatctt cgagaaggca aagcagtaca cgggcgtctc tcttggtgac
    14941 tccgagacta cagaccgcgc ccttcgcatc gtcgccgacc acgcgagggc catggccttc
    15001 ctcatcgccg acggcgtcag gccagggaac cagcgcagag aatacgtact tcgccgcata
    15061 atacgcaggg cgatgttgca ggcacattcg cgcctcggca tgagccctga ccagctcgcc
    15121 gggttggctg aggtagtggt cgagtacatg ggtgacttct acgaggagtt gcggtcagcg
    15181 cgtgaggaca tacgccgcat cgtcgccggc gaggccacga ggttcgtcga gatctacgag
    15241 tcggggatgt cgcttctggc ttccgagata gggcgcatgc ggggcgggca cttacctggc
    15301 gacgtggcct tcatgttgca cgacacgtac gggttccctg tggaggtgac gcgggaggtg
    15361 ctcgccgaga gaggtttgtc gctcgatgag gcgggtttcc aggcagcgat ggacgcgcaa
    15421 cgggagcgtg ccagggaggc catgcggggc tacgagcggg tcgttgccgc gttcagggat
    15481 caggagatca gcagccgctt tgtcggctac gagcgcgagc aggtagagag taggatagtc
    15541 gccgtcgagc ccgtgccgga cgccggggga gaggtctacg tcgtattggc ggagaatccc
    15601 ttctacgcca caggtggcgg gcaggtggcg gacgagggtt ggatcacctc ggaggatgga
    15661 caactcgagg tcttcgatgc gatcccggcg ggtgactatc aggtgctcat ggcgaggata
    15721 gaacgcggct cgttccaggt tggggatgcg gtgagggctt ctataaacag ggtgcgccgc
    15781 cagcagatag aggcgaatca cactgccacg cacatcctgc actgggccct cagggcggtc
    15841 ctcggccagg acgtcgtgca ggcaggcagc tacgtcggtc ctgacaggtt gcgattcgac
    15901 taccgctacg ctgggaaggt cacagacgat gaggtcaagc gcgtccagga gctctcacta
    15961 cagaagataa ccgaaaacca gcccgtgcgg tactttacga ccacgctaga ggaggcacgt
    16021 aacctcggcg cgatgatgct cttcggggag aagtacgctg acctggtacg cgtggtggag
    16081 atcgatggat tctcgcgcga gctgtgcggc gggacgcatg tgcgtggcac cgccgagatc
    16141 ggggctttca agatactctc caacaggaaa cacggcgccg acctctaccg tatggaggtc
    16201 ataacaggta gggaggctct ctactatact accggggcga ccgagaaggc agaagaggtc
    16261 tcggaggccc tgaagatcga gatcgaggaa ctaccagagg ccgtacagcg gctccgcgag
    16321 gaggtacagg cgtctcgcga ggcggccagg gagcaggcat tgaagcaggg tctcgaggag
    16381 gtcggcgacc tcgttgaagg cgcccagcag gtcaacggaa cgagggtggt aacgggccag
    16441 gtcgtcgccg ccgacgtcag gggactgcgc cagatctccg acgacgtcaa gaatcgtctc
    16501 agcggtcctt ccgccgtggt gctggccgct gcgctcggag gcaaggcggt tatcgtcgcc
    16561 aactttcacc ccgaggtctc ccgtagggtc gaggccgggg agatagtacg cgaggtctct
    16621 ggcgtactgg gaggaggcgg gggtggcggc gcgacgatgg cccaggccgg cggtggcaac
    16681 ctcgaggcca tccccgacgc cctcgcgagg gtacgcgaga tcctcgacca acggctcgcg
    16741 gacccggagt aagtcctgtc gccagccgac cgcgggcgag tcgccgccct cgacctcgga
    16801 gaggtgcgca ccggcatcgc cgtctcggac cccggaggcg ttatcgccag gcctctcgac
    16861 gtgataccga gcggggatct ctccccttac ctacgccggc tcgtggacga aaagggcgtc
    16921 tacgaggtcg tcgtcggcgt gccgaagacg ctcggtgggg aggtcggctt ccaggcgcgc
    16981 aaggttttgt ctagacttga cgccttgaag agagagttcc ctggcgtgcg gttcgttgag
    17041 tgggatgagc ggttcacgac ccgtctcgcg gggagccgcg gcaagagaaa ggggaccgcg
    17101 agggtggatc acctggcagc cgcacggatg ctgcaggagt acctcggggc gagggagagt
    17161 ctttgaggcg ccacgacaag agcgagcgag gagaccgtga ggaactccat gccaggctcg
    17221 acgccctccg gcgatccggc tccaggggga tccggcgcaa acgccgctcg aacgcaggac
    17281 ctactgcgct cggcgtcctt ctcgtgctcg gcgttctggg ggccatcttc gtcatctact
    17341 ccgcggcgac aggaggcgag gacgtgtctg gggaatcggc ggaggtacag gtcgtcaaag
    17401 gcgacacgct ctcggacgcc gccgccaagc tggaagaggc cgggatcata aagaacgcct
    17461 tcatcttcga gctgcaggcc aggtatgaag ggtacggaac ggagataaag accggacgtt
    17521 acactttcga gccaggccag gatagcgggg agatcctcca taaactgacg gtcggacagg
    17581 ctgcacctac gttcaccgtc acgatcccag agggtctcac tatcgaggag accgccggga
    17641 ccgtcgccgc ggacagcagt gtccccgcgc ccgagttcga ggaggccgcg cgtagaaccg
    17701 aatacggata cgcctttctc gacaactccg acgtcaagac aacagaaggg tacctctttc
    17761 ctgccaagta cgatttcgag aagggcgtta gcgctaggca gatagtcgac aggctcctcg
    17821 agcagtatct tctcgtgacc cagagcctcg acttcgccga cgcgaaaaat cgtctcgacc
    17881 ttacggagaa tcaggtcgtg accgtggcct ccctagtcga gaaagaggcc gccagccccg
    17941 aggagaggcc gctgatagcg tcggtgatct acaaccgcat gcgcaaagac acaccgctcc
    18001 agatcgacgc cacgatccag tacgcgctca agagacccaa ggcgaacctc tcgctggacg
    18061 atttgaaggt tgactctccg tacaatacct acgagaacaa aggactgccg ccaggaccca
    18121 tatgcagccc cggacgggag tcactccagg ccgcgctgaa ccccgcgaat accgactacc
    18181 tctactatgt cttggaggcc aacggtgaga agcacttctt taccaagagc tacgatgatt
    18241 tcctcaggga gaaggcgaag gcaggacgct agggaagctt gcaaagaagt tcaggacctc
    18301 tttgcaagca tttcagcacg cttcctccgg ctgacaatcc gcctggcgca gcctggcagc
    18361 cggtgtcatg ggtatctcat cttgcaaatc tgcgactttt cgtggaagat ccctctttat
    18421 agggccgatc cgcgtgggag tgcgttcact cctggggcgt tcgagcccgc cgaaactcct
    18481 tgacggagca tatgttgcgc gcgactggag atcttcgtgg cttccgctcg tgtgtaaggg
    18541 gagcgggccc ggcgcctact ccccttcagt gctggcgaga gacgagaggg cgaatttggg
    18601 caagcagtcg agcaatggga gacttggagc agggatcaca cgccggaggt ttctccgggc
    18661 cctcacggcc ggaaccgcct tggtcgcact ttcaggctcc cttggttgcg agccgagctc
    18721 gcggaccagg gcgtccgccg cgccccaggg acctggacag gccaggactt tccgctcacg
    18781 cccggatctc cgtccgcctg ccatcaaggt gaacacgaat ctgcctggcg tggcccccgg
    18841 ctacatcttc gtctcgccca aaaagggtcc aggagaggaa gctcccaccc aggatgcacc
    18901 gctcatagtg gacgccagcg gtgagcctat ctggttccat ccgctgcagg atgccgaggc
    18961 tgacgctttc accttcgagg tccaggaata caagggagag acggtgctca cctggtggga
    19021 gggactccat acaggatacg gccagggcga gtacgtgatc ttcgaccatt cctatcgcga
    19081 gataacccgg gtcagggccg gcaacggcta ccagggggac caccacgagt tcctcatcac
    19141 gcccgaggat acggccctca tcacgatcta cagcaaagta caaagggacc tgtccggcgt
    19201 cggtggtcct gtagacggtg ttgtgctcga cgggatagtc caggagatcg acatcgagag
    19261 cggcgaggtg ctcttcgagt ggcacagcct cgaccacatc ggcttggagg agtctctcta
    19321 cgaaccgccg cccgacctca acgcgggttt cgattacttc cacatcaact ccatagatcc
    19381 gattccaggc ggatacctga tcatctcggc ccgcaggact tgcgcggttt acaaagtcga
    19441 ccgcaagacg ggagaggtgg tctggcgtct gggaggagaa cagagcgact tcgacatggg
    19501 atacggtacc agaaccgact ggcagcacga tgcccgccgc cactccgacg gcacgataac
    19561 gatcttcgac aacggcgggg tccagaaaga cgtacagtcc cgcggcatcg tggtggaggt
    19621 cgacgaagat gacatgagcg ctaccctggt aggcgagtac acgcatccca acaagatact
    19681 cgcagccacc cagggtaacc tccaggtgtt gcccaacggg aacgtgttcg tagggtgggg
    19741 cagcgagccg tcctactcgg agttcggccc ggatggcaaa ctgctctacg acgccagctt
    19801 cccctcccag gtcgagtctt acagggcctt ccgctttccg tggaagggtc aaccggagga
    19861 tcggccggcg gtcgtggccg aagccgggcc ggacgacaag gtaacgatct acgtcagttg
    19921 gaacggcgct acagaggtag atacctggca ggtgctggcc ggtggaaatc ctgaccagct
    19981 cgagcccgcc ggctcaggtc cccgcaaggg cttcgagacc gccgtaacgc tgtataccaa
    20041 tgagccttac gtcgccgtcg aagccaagga cagctctggc cgggtactag gtacctccga
    20101 ggccgtcgag ccagtagagc cttccgcttc gacggaccga acccgctcgt aaggtatcct
    20161 gcaggacggg atagtggaca aaagccggat acagcttcga tggttcgagg ccggcgtcgc
    20221 gctcgcggtg atcgcggctt tcgccgctat cggcccgttc agggatacgt ttgcgccgat
    20281 agcttgggtc atgttgctct gtaccctggt cttgttcttg acgccgggcg tattactggt
    20341 gcgctggttc ttgagggact attttcacgg cgccgcgctc gctccggcgg cttgcgttac
    20401 gagcgtcggg ttcttcgcgc tcctagcagt gcccatgctc atcttgcaga gcaccctgga
    20461 agcctacctg tgggcctgcg gcacgatcgt agccgcgtgt tggctggctg ctgtggtttt
    20521 gaccttccgc cccgagcagg cgcatggtgg gcataccggc ctcgcgctct cggaccgagg
    20581 gggattgctc tgggcgcctt tcgccgtcct tgtcgcggcg ctctcgtacg tagccaggaa
    20641 cacggccccg agctactacg gtgacatctg gatctacctc tcgtgggtca gagagtatct
    20701 tggcggagac aggctcgcct ccgccgagcc gttcttcggt agtgaggtgg gcctctcgcg
    20761 ggccaggatc aatggctggc tactggagca ggcagcggtc tccagggtct ctggtgtcga
    20821 ccctgtagac ctggtcttct catacctgaa cccggcactg gtggttgtcg ccttcctggt
    20881 cttctacgcc ctggcgcgca tcctgttcga gagcgagaaa gcggcgctgt tgtccggttg
    20941 cctgtacgct cttttcttcc ttgtacacct cagccagtcg cgcataacct gggggggcga
    21001 gtttgtacag cgcttgcccg aggacaaact ggtcgccaag ttcctcttct tgcccatggc
    21061 cctcgctttc gcttacgcgt ttctgcaagg tgggacgaag cggtactttc ttggtttctc
    21121 gttcctctgc tgtgccgtca tggccgtgca tcccataggt ttggcgatca tcgggatctc
    21181 catggccggg tgcgccattt tgcaccttgc cgccaaccct cgcagccgcg cggcatgggc
    21241 caggatctcg gccatgggcc tcaccggtgt cgcgatcatc gtcgttccgg cggtcctcgt
    21301 ctcgtcgttt accgatgagt cgctgtcgaa cgcgctggcc gactcagaca tcaattccgg
    21361 cgaccccgac gtgctgcgga acatgatctt cgtcagtccg gagaggaacc gcatcttcga
    21421 actggctgac ggctcttaca ttatgcaccc ttctttgatc ctcgatcctg tgatcgccac
    21481 cgccttcttg atcgggcttc ctttcttgct ctggcgcctg aagcggagcc tggccgcaca
    21541 attgctcttc ggaatgatgt acgtgacgac cgtaatcgtc tacgtacgcc cgatcgccac
    21601 gttcctcggc gataatgtcg tgctcccggg ccagatctgg cgtctggcct ggccggtgca
    21661 gctcgccgcc gtactgacgc ttggctggct cgtgtggaca gttacggact acgtggccgc
    21721 gtggctgcga agcctcgggc cggcgcgttt tctggcgggg gcactaccga tcctactggt
    21781 cgtcgccctg acggccgctg ccgtgcccca ggcgcgtcaa ggtatggagt ctattcaggc
    21841 gcacagggag gctagccgca aggctggctt ttacccttct gatcccatct ttccctggtt
    21901 tcgcgacgag atgcgctcct ccgttgtcgt gcttgcgccc gacatccagt gcgcccgtat
    21961 ccccgcttac tcctccgagg ctaacgtcgt cagccgcaga ggaggattgg tgctgagagt
    22021 cctccccgag ctacagaagc gggcgccggg ccagatcgag gtgccccagg gttcgctgga
    22081 tgtgcaggag ttcttcagcg ggactacgct gcagaaagcc gtcgagatcc tgcgccgcaa
    22141 caacgtggac tacgtgatgg tcgcaaaaga ctcacagttg accggatcgc tccaacgcct
    22201 gtccggcttc accccagtcg agacaccgag tgagaggtac gatctttacg cagttagcct
    22261 ccaggaggta gcctcgaagt caggaccgcc gatgagttcg cctggggccc tttctgtaga
    22321 atggccctct ctggatactc gattcgggag gcagatctct tgacgttttt cggtaggctc
    22381 ggtgacaggg tgatcggcgc cccatcgata ctggcggccg atttctcgga actggccgag
    22441 gaagtgcgga gggccgaaag gggtggagcc gagctcatac acttcgacgt gatggacaac
    22501 cacttcgtcc cgaaccttac cgtggggccc gctgtggcgg cctcgctcgc cgccgcggtc
    22561 gacctaccca tcgacgccca cctccaggta acgaaccctg acaacctgat cccatctttc
    22621 atcgaggccg gggtcgtcag catctcggtc caacccgaag tcgtcacgca cctgcaccgc
    22681 acgctcgacc tgatccgaac cggcggttgt gaggcaggcg tcgccctcaa cccgacgacg
    22741 cctcccgagt ctatagagtg ggcgctgccg tacgtgagct acgtgaacgt aatgagcgtc
    22801 aaccccggct tcggaggcca ggcctttatc cccgagatgg tcgagaaggt caggcacctc
    22861 aaggagaaga ccgacgtccc cgtcgaagtg gacggcggta tcacggtcga gaccgcgccg
    22921 ctcatggtca gagcaggcgc ccaggtccta gtcgccggtt cggcggtctt caaaggcgat
    22981 ccagcggcgg agatgcgcag gattatcgaa gccggccaga gagctagtca gcactagctg
    23041 aaatgccaaa acctttcctt gctgccttgg gccttctgtg gtatagcttg ctgcgtaact
    23101 ttcgggggcg ggtggaattc ccgcaccggc ggtgagcctg acagggtcgt cttgtgtccc
    23161 tgccggggag cccgcgagcc tccttggagg ttgagccggt gaaggttcgt atccggtgcg
    23221 aaccgtagtc cggcgccgac ggtcagagtc cggatgggag aaaggaaaac gtgctgcggg
    23281 attctatgat ggatcttgcc cgttgcctcg ccgagcgcgg gcgttactcg gttgcgccga
    23341 accctctagt gggggccgtc gtcgcccgcg acggcagctt gctaagcgag ggctggcatt
    23401 ccagggcagg agaggaccac gccgagatag tggccctatc tagggccgga ggggcttcgc
    23461 gtggggccac gctctacgta acgctcgaac cctgcaacca ttacggcagg acgccgcctt
    23521 gcgtcgaggc catcatgcgg gccggcatct cgcacgttgt cgtcggccac ctagaccctg
    23581 atcccaggat gcagggcagc agcgtccgga tactgcgcga ggccggcgtc gaggtcgatg
    23641 tactggacga ccctgcgttc gagcggcaaa acgaacagtt cctccaccac atgcagaccg
    23701 gaaagccgtt cgtccacctc aagctagcta ccaccctcga cggtaggatc gccgcgtccg
    23761 gcggtgacag caggtgggtc acgggcaaag acgcgaggct gcgggcccac gagctcaggg
    23821 ccgaggccgg ggcggtactg gtcggcgcga acaccgtgcg gacggacgac cctatgctta
    23881 cggcccggga tcttcctgta cggtcacccc gtataacccg cgtggtgctg gaccctcatc
    23941 tgacgatcag ccaggagagc aagctcgcca agagcgcggg agagactccc gtcgtcgtct
    24001 tcgcctctga gcccgcgctc ggcggtcgtg aaaaggagtt ggagaggctt ggtgtcgagg
    24061 tggttgtgat cccgctttcg gaggagggtc tcgacctcgt ctacattctg gatgagttgg
    24121 gcagacgcgg gttgcgaggc ctgctcgtcg agggcggggg agagacggct acgcgattcg
    24181 tccgtatggg gctcgccgac aagctgaccc tgttctacgc gccgaagctc ctcggatctg
    24241 agggcgtgcc catgatcgga gcgctcaggg ctacgaaggt ggcagagtcg ctgcaattct
    24301 cggtctcgga ggttgggaga gtaggcgatg acgtcgccgt aacgctctat cctggcggag
    24361 aggaggaacg tgttcaccgg gcttgttgag gaagttgggc gtgtctcctc gatcggggag
    24421 gacgagatgt tgcgcttgag catctccgtc ggcagggtgt ctgagggcac ccgcgctggc
    24481 gacagcgtct gtgtgaacgg ggcgtgcctg acggtcagcg aggtcgatgg catgacgctc
    24541 actttcttcg ccatgcccga gacgttgagg cgcacggcgc tcggggacct cgccgtcggc
    24601 agtcctgtca acctggagcg ggcgatgtcc gcggggagca ggttcggagg acacatcgtg
    24661 cagggacacg tggacggcgt tggcgaggcg ctcggggtta ggcccgaggg cgacgcggag
    24721 atctgggagt tcggggctcc cgggacagtg ctccgctatt gcgtcgagaa gggcagcatc
    24781 tgcgtcgacg gtataagcct gacgatcgtc tcgatcggag atggcacttt tacggtctcg
    24841 atactgcccc agacccgcgc gaacacgaac ctgggggagc tcggggcagg gagcagggta
    24901 aacctcgagg ccgacattat aggtaagtac gtagagcggc tgctcgagcc gcgcctggta
    24961 gggagacttt caaccttcga gaggagtgcc gcagatgcct tttagcccta tagaggcgat
    25021 cgtcgaggac atcaagcagg gcaagatggt catcgtttgt gacgacgagg accgtgagaa
    25081 cgagggcgac ctgaccatgg ccgccgagct tgttactgcg gacgacatca acttcatggc
    25141 cgtccacggt cgcggtctga tctgccttcc gatggcgggg gagatcgtcg accgcctcga
    25201 cataccacag atggtcaccc acaacgcttc gaggatgggt acagcgttca cggcctcgat
    25261 cgaggcgaag gagggcatca cgacgggcat atccgccgcc gacagggccc acacctgcag
    25321 ggtcgccgtg gacgaggcga ccggcccgga agacatcgtg atgccaggac acgtattccc
    25381 tttgcgggcg aaggccggtg gtgtcctgca gcgcgccggc cagacagagg ccgccgtcga
    25441 cctcgccagg atggccgggt tcaagcctgc gggtgtgatc tgcgagatca tgaaggagga
    25501 tggcacgatg gcccgtgtac ccgacctcga gaggttctcc agggagcacg gcgtcaagct
    25561 ggtgacggtg gcccagatca tcgagtaccg tcacgccttc gagacgcagg tcaggtgcgc
    25621 cgtcgaggcg cggctgccca ccccgttcgg ggagttcagg ctgcgcgcct acgagaacga
    25681 catagatgaa cttacccacg tcgcgctcgt catgggcgag cccgagggta aagacgatgt
    25741 gctcgtcagg gtccactcgg catgcctgac cggcgacgca tttcactccc tgcgctgcga
    25801 ctgcggcgaa caactggagg ccgcgatgag ctccatcgcg gcggaaggcg aaggcgtcat
    25861 tgcatacatg cagcaggaag gtcggggcat cgggctcttg aacaagatga gggcctacca
    25921 tctgcaggat gaggggatgg ataccgtcga ggcgaaccag aagctcggcc tcgcgcccga
    25981 cctgagagac tacggggtcg gggcccagat cctcaaggat ctcgacctca agcgcatccg
    26041 tcttcttacc aacaacctga cgaaggtcgt cggactcaga ggcttcgggc tcgagatcac
    26101 cgagcgtgtc ccgatcgaga tggagcccaa cggccacaac gagcggtacc tgaagacgaa
    26161 gcgagagaag ctgaaccacg tcttcgagaa gttctagagt ggtttgcgaa gaggtctatc
    26221 ctctttgcgg gcatcaggat ctttctgcga ccagcaggtg atgtgtacta ccctggtgga
    26281 ccaagcaatc cggagattac tgtaggaggc tagcgtggag ggagtcaacc acatcgaggg
    26341 gaacctctct gctgagggga gatccttcgg gatagttgcc tcgcgcttca acgattttgt
    26401 cgtcaaggcg ctgctggaag gggctatcga ggcgatccgg cgccacgggg gcgaggcggg
    26461 ggccgctgac gtcgtatggg tgccgggttc ctacgagata ccggtcgtcg ccagagagct
    26521 ggccctttcg ggacgctacg acgcgctcat ctgcctaggg gcggtaatac gtggcgcaac
    26581 ggcccatttc gactacgtcg caggcggggc cgcaggcggc atctcgaatg tggcgctgga
    26641 gaccggggta cccgtgatct tcggggtcat taccacggag tcgatcgaac aggccataga
    26701 acgcgccggc acgaagatgg gcaacaaggg tttcgaggcc gccgtgtcgg cgatggagat
    26761 ggctgacctt atggcgaagc ttcgtgaacg cgcctcgctc agcaacccct gagggctaga
    26821 cggcctgcga cgcggaagat ggcgctccta tggaggcctt cttcaccttg aacctcttga
    26881 ggcatgaggt gcaaacccac tcacgccgcg tggccgagcc ttcctggata cggaccttct
    26941 gcaaattcgg gtctctgcga cggcgcgtcg ccctcagcga gtggctgcgc gcgttgccga
    27001 agtctggctt ctttccgcat gaataacaaa ctttcgccac gaaacttctc ctgtgctagg
    27061 ataccggtcg aatttgggga ccccggaaaa cgcgactcgc aatccgcatc cggacgtcgg
    27121 gcggtgattg taccagattg tcgacgatgt cctacagtgg ggagtggtct accggaagcc
    27181 tctcgagagg agcaatgagg gattctttgc gatggcgata gaggtggcct acatggttgc
    27241 tgcggcccac gcggccgtca aggcccaggt cgccaggatc aacgatctca acgtctatcc
    27301 cgtgccggac ggggatacag gcacgaacat gttgctcacc ctggcgtcag cgttgaacga
    27361 gacgtcgggc aagtcttacg gtagcgccca ggaggcgagc agggcctgtg ccaggtccgc
    27421 gctgatgggc gcgaggggaa actccggggt catactctcc cagatgatcc gcggcgcatg
    27481 cgaggtactg gccgacagga gatccctgga cgccgatgcc ttcgccgccg ggctggaagg
    27541 cgcgagagag cgggcttacg cctctgttcg cgaaccggtg gagggcacga tgctcaccgt
    27601 aatcaaggat gcggccctgg ccgcccgcga ggccgccgaa ggagggaacg ccgacctgcc
    27661 ttcggtggtc ggggcggcga ggcaggcggc ccacgattcg gtgcgccgca cgccggagct
    27721 gctcgctgtc ttgcgcgagg ccggcgtcgt cgatgctggg gggttgggtg ttgctgtggt
    27781 cctcgacggc gtctacgcct gcattagtgg gcaggagatc gaggtccccc aagcggacga
    27841 ggacgacgcg cccgacctgg aggctatcca cgcccaggaa gcggcctggg gctattgtac
    27901 ggagttcctc gtcgacggct tcgaaggcga tgtggaggat ttcaaggagc acgtatacgc
    27961 ctcgggcagg agcgtcctcg tggtcgccca agacgacgtc gtcaaggtcc acgtgcacac
    28021 gcaggaccca ggcggtgccc tctcctacgc gggcggcttc ggccgcctgg ccggcgtgaa
    28081 ggtcgatgac atggaggccc agatccgctc ccgcgggccg gaggacaggc cagcagccag
    28141 attgggcgtc gtcgctgcga gccgggggga gggcaaccgc gccctcttcg agcagatggg
    28201 ggcagtcgtg atcgagggcg ggcagggcga gaacccgagc gcagccgacc tcgccggggc
    28261 ggtagatgag acagggtcct cagccgtcgt gctgcttccc aacaacaaga acatcgtccc
    28321 cacagcggcg caggtcagcg agcttgtaga ggcgaagacg tacctcgtgc ctactaccaa
    28381 cctcgccgct ggtctcgccg tgatggtcgg gtacgacgcc gagggcgaac ccgatgaggt
    28441 ggtagaggag atgtgtgaga tctcagactc cctgcgcgtc ggcgaggtta cgcgttccgt
    28501 gcgcgacgcc cgcatcggag accgcgaggt ccccgaaggg gcttatatag ggttcctcga
    28561 cggcgacctc atggccgtcg agaatggagc ctgccagacg gcactcgtgc tcgtgaggaa
    28621 gatcgttggt gacggcgccg acatcttgac gctgctcaag ggcgaagagc tggccgaggc
    28681 cgagctcgaa aggatactcg acggaatcag agaactcgac gacgacctgg aggtcgagac
    28741 gagggatggc ggacagccgc tgtatcctct acagatggtg gccgagtgac cacggcggtc
    28801 gtaaccgact cgaccacgag cctggcccct ggtgtgcttg agcggcctga tgtcagggtg
    28861 gtcccgctca cgttccactt cggtgaagac gaggcgtata cggacaaggt agacatgacc
    28921 aacgaggagt tctacgagcg gctgcgcgac tccgacctct tcccgacgac ggcgcaaccc
    28981 tcccccggta ccttcgtcga ggcctacgag gcgctctctg actacgacga catcctcgtt
    29041 ctcacgctct cgagcaagtt cagcggcacc tacgactcgg ccctgcaggc ggcggggatg
    29101 gtggacagac cagtccacgt actcgattcc aagagcgccg agatggggtc tgggttgatc
    29161 ctgctcgagg ccctgcgctg tatcgacgag ggtggggact tcgacgaggt cacgcatgca
    29221 gctgtgcggg ctatcggagg gacgagggta ctcttcgcag tgggcacact ggagtatctg
    29281 gccaagagcg ggcgcattgg aagggcgcag cgtctcctcg gcacagccct cgatgtcagg
    29341 cctgtgctcg agataggggg cggggagatc attcctttca aacgtacccg cggccgcagg
    29401 cgccagatgg cgaccatcgt cgaggaggtc aggccggccg cggaggcagg acgtcagctc
    29461 tacttcgggc acatcgccgc cccagagctc ctctccgagc tggccgaaca gctcggggtc
    29521 gaggagaaac tagtggctga gatcgggggg gtcgtcggtt cccacgtagg ccttggagcc
    29581 tatggcgttg cccacttata ggggcggcgg ggcgcagggg gctcctctcc ccgcgcgcac
    29641 gaccctgacc gaccttcgtt cgcgtcctgt gaccgagctt cccggcgtcg gaccgaggat
    29701 cgaggccgca ctcggcgacc tcggcatagc ctcgctggcc gacatgatct cgcactaccc
    29761 atcgcgccac gaggatctgt ccaacgtgaa gaggatctct gagttgcgcg tgggggagag
    29821 ggcaacggtc ttcggcacgg tggtctccac gcgccccgtg ggcaaacctg tcaggggacg
    29881 agcccccggc ttctcggccc aactctacga cggcacggga tacatccccg tgacggtctg
    29941 gggcaggagc tggcttatga accagctgct ccccgaggtt cgggtggtcg cttccggcga
    30001 ggtccagagc cgctacggta ttcagatagc cgcaagaagc atcgaggtcg tcgatgatcc
    30061 aggggcggac gaaggaccgc acgctggacg cttcgttccc gtctatccgg tcaacaaggg
    30121 tatacaggcg cgcaggatgc ggacgctcat ccaccgcgcc ctcgacacgg ccggacgcat
    30181 cctcgacccg ctgccagccg a
//
LOCUS       DASTKM010000110         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_202116, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000110 DASTKM010000000
VERSION     DASTKM010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202116"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFQ10_04775"
     CDS             <1..255
                     /locus_tag="VFQ10_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HET9964687.1"
                     /translation="QAKDDKVDLLYSESMNLIANPSEGGGGAPEWRMRAALMVHYLER
                     ISDHGVDIGGSAVFLVTGQRIEDAMQRYLDRNVDHDGDGT"
     gene            419..913
                     /locus_tag="VFQ10_04780"
     CDS             419..913
                     /locus_tag="VFQ10_04780"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HET9964688.1"
                     /translation="MSDNPMFLYAGEYENVDDAKADLETLKEMHREHIVGTYDAAVVT
                     KNEEGKVKIVDKIEKPTQHGGWAGMAVGAAMGLIFPPSILVGGLLGAGAGALIGHLEG
                     GMSRQDLKEVGEMLDNSEAALIVVGEATVERAVDEATERAKRDLKKEIRADAREMERA
                     VDKA"
     gene            1024..1473
                     /locus_tag="VFQ10_04785"
     CDS             1024..1473
                     /locus_tag="VFQ10_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9964689.1"
                     /translation="MRLTQKSKYAVRALMELALDERDCPLGVAELARRQHIPERFLEQ
                     IFGELRRANIIESRRGAHGGYRFAMPTEEISVLDVVEILDGEIRPARCSAGGVCYIAD
                     APLCATSKVWDEAREALEDVFGRYSIAALAATEREERASHEAAPAGR"
     gene            1663..2139
                     /locus_tag="VFQ10_04790"
     CDS             1663..2139
                     /locus_tag="VFQ10_04790"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9964690.1"
                     /translation="MARHLSKRDLAGTHAIELGCGVGLPTILALAQGAEVLATDHYEA
                     ALDFTTYNARTNLHREPKVSVLDWREPDTEGIGTYDLIFAADVLYERKNAAAIADLVP
                     KLLAPGGEAIFADPHRDEAPVFLEAMERYGFEDATEEITVEQPASGVKVLLHRLRR"
     gene            complement(2199..2450)
                     /locus_tag="VFQ10_04795"
     CDS             complement(2199..2450)
                     /locus_tag="VFQ10_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964691.1"
                     /translation="MNPRDLPPTGPRPLWDVLWVVGHPNDQRTDRILSTYDVRYIVLY
                     KNMPDRLTQNYWKLFEDHLDLYRITFENDDVLIVARRGT"
     gene            complement(2575..>3585)
                     /locus_tag="VFQ10_04800"
     CDS             complement(2575..>3585)
                     /locus_tag="VFQ10_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964692.1"
                     /translation="LTAMISRLGGLEPLEIFPVLGPALLLLPALACYTLARGLWGWPY
                     GVAAALLAGVLNGGTYYFNDAKYPNLVTSQFLLVVIIAALTGFYDAPSPRRGLALALV
                     GSSVVLYHPVVSLYEAALLVLVSVYFLPYLLAHERAKGLAMLSSLVLLGSLSILYAWD
                     TYDLPQTLAGLMGASEASTTDTAVGIAVGTQVPYPLDLLVGTVVTQPVAWLGLLGALL
                     VMTELLGRRVSKPQALALFTVLLWASLLFLGSRMPLTGFPQRFGRDVGIPLAILAALS
                     FVTILRSLGAPRRSQAVFVASLVVLLTGSLVGYRAVQSLESASAKSAQLTITPQIAAA
                     GE"
BASE COUNT          745 a         1058 c         1180 g          602 t
ORIGIN      
        1 caggcgaagg acgacaaggt ggacctcctc tactcagagt ccatgaacct catcgccaac
       61 ccgtctgagg ggggaggcgg cgcgcccgag tggcggatgc gggcggcctt gatggtccat
      121 tacctggagc gcatctccga ccatggcgtg gacatcggcg ggagcgccgt cttcctggtt
      181 accgggcagc ggatcgagga cgccatgcaa cggtacctgg accgcaacgt ggaccacgac
      241 ggtgacggca cgtaacagag tagatcctgc gtgctccgtg caatagtagt ctagactacg
      301 atcgacgtgg aggcagaaca accagggaga tgagggaaac caggatagct ctcatgcaag
      361 accggagaat cgcaaggtgg gcgagatgga ccgacggaga atgggaaaga agtgctctat
      421 gagcgacaac ccgatgttcc tttacgcggg cgagtatgag aatgtcgatg acgccaaggc
      481 agacctcgag acgttgaagg agatgcaccg ggagcacatc gtgggtacct acgatgcggc
      541 cgtagttacc aagaacgaag aaggcaaggt caagatcgta gacaagatag agaagccgac
      601 ccagcacggt gggtgggctg ggatggctgt tggggcggcc atgggcctga tcttccctcc
      661 ctccatcctg gtaggcggtc tgttgggtgc gggagccggc gccctgatcg gccacctgga
      721 gggcggcatg tcgcgccagg acctcaagga ggttggggag atgctcgaca acagcgaggc
      781 ggccctgatc gtggtcgggg aagctacggt cgagcgggcg gtcgacgagg cgaccgagcg
      841 ggccaagcgg gatctcaaga aagagatcag ggccgacgcc agggagatgg aaagggccgt
      901 cgacaaggca tagccccaag ggaccgagac ccccggcatg cgcgcatgcc gggggtcgtc
      961 acctgcgtat ttctgctaca atctcttctg gcggtacgct tctgttggtc gggtgattat
     1021 ctcttgaggc ttacgcaaaa gagcaagtat gcggttcgtg cgctcatgga gctggcgctc
     1081 gatgagcgag attgcccgct cggggtggcc gagctggccc gccgccaaca catccccgag
     1141 aggttcctcg agcagatctt cggcgagctt cgccgggcga acatcataga gagccgccgc
     1201 ggggcgcacg ggggataccg cttcgcgatg cctacggagg agatctccgt cctcgacgtc
     1261 gtcgagattc tggacggtga gatccgtcca gcccgctgct cagcgggcgg ggtctgctat
     1321 atagccgacg ccccgctctg cgccacgagc aaagtctggg acgaggcccg cgaggcgctg
     1381 gaggacgtct tcggacgcta cagcattgcg gcactcgccg ccaccgagcg tgaggaacgc
     1441 gcttcccacg aagccgcccc cgcaggccgc tgactaaccc tggataggct ccgagcccat
     1501 ctccgcggcc ttaccgggct ggccgaatcc acggtcgagg tcggggacag gacataccgc
     1561 ataacccacc ccgtctcagc ggacgacctg atcgacgagg aagacttcga ccgcgatgag
     1621 aggctccctt actgggccga actctggccg agcggcctcg cgctggcccg tcacctctcg
     1681 aaacgcgacc ttgcgggtac tcacgccatc gagctggggt gcggcgtcgg gttgcccacc
     1741 atactcgcgc tcgcccaagg tgccgaagtc cttgctacag accactacga ggccgctctc
     1801 gatttcacaa cctacaatgc ccgcacgaac cttcaccggg aaccgaaagt ctcagttctg
     1861 gactggcgcg aaccggatac agagggtatt ggaacctacg acctgatctt cgccgcggac
     1921 gtcctctacg agcgtaagaa cgctgctgcc atcgccgacc tcgtaccaaa gttgctcgcg
     1981 cccggcggtg aggcgatctt cgccgatccc caccgtgacg aagcgccggt ctttcttgaa
     2041 gctatggaga gatacgggtt cgaggatgca acggaagaga tcaccgtcga gcagcccgcg
     2101 agtggggtga aggttctcct gcacaggctg cggcggtagg ccaggtctgg cctgcgagag
     2161 taacttgccc tttcgctcgc gcttaccgag tacctggttc aggtaccgcg ccttgcgacg
     2221 ataaggacat cgtcgttctc gaaggtgatc cggtaaaggt ccaggtggtc ttcgaaaagc
     2281 ttccagtagt tctgcgttag cctgtcaggc atgttcttgt agagcacgat gtagcgcacg
     2341 tcgtaggtgc tcaggatccg gtcagtacgc tgatcattag ggtgccccac gacccagaga
     2401 acgtcccaaa gtggtcttgg ccccgtgggt ggaaggtctc tcgggttcat gatctgacgg
     2461 ggctcgaagg attgtagagc cgagtaatgg ccctatggcc agcatcatgc ggctaggcac
     2521 ctggttggcg tgcgggctca ctatgatgtt gccaccgtcg ttgttctctt cgagttactc
     2581 tccggcggcg gcgatctggg gcgtgatcgt aagctgtgcg ctcttagcgc tggcactctc
     2641 cagactctgc accgccctgt accctacgag acttcccgtc aggagcacca ctagggacgc
     2701 aacgaagacg gcctgggatc tcctgggggc tcccaacgag cgtaggatcg tcacgaagct
     2761 gagggccgcc aggatggcaa gcggaatccc cacgtcacgc ccgaagcgtt gcgggaaacc
     2821 ggtcagtggc atgcggctgc ccaagaacag aagcgacgcc cacagcagca cggtgaaaag
     2881 tgccagtgct tgcggtttac tcacgcgcct ccctagcagc tcggtcatca cgaggagcgc
     2941 gccaagcaaa ccgagccacg caaccggttg cgtgaccact gtcccgacca gcaagtcgag
     3001 tgggtaaggg acctgcgtcc ccaccgctat gcccacggcg gtatccgtgg tgctcgcctc
     3061 cgacgcgccc atgaggccgg cgagcgtctg cgggaggtcg taggtatccc aggcgtagag
     3121 aatggagaga gagcccagga gcacgagtga agagagcatc gcgagcccct tcgcgcgctc
     3181 gtgcgcgagc agataaggca ggaagtacac tgaaacaagc acgagcagcg ccgcctcata
     3241 caggctaact accggatggt agagaactac tgaggagcca accagcgcca aagcgagacc
     3301 tctgcgcggt gagggcgcat cgtagaatcc ggttagcgcg gctatgatga ccacaagcaa
     3361 gaactgcgag gtgacgaggt tcgggtactt ggcgtcgttg aagtagtagg tgccgccgtt
     3421 gagcactccg gctaagaggg cggccgcaac gccgtagggc catccccaaa gccctcgcgc
     3481 aagggtgtag caggccagtg ccggtaagag gagcagggcc gggcccaaca ccgggaagat
     3541 ctcgaggggt tccaacccgc caagacggga gatcattgct gtaag
//
LOCUS       DASTKM010000111         4624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_554316, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000111 DASTKM010000000
VERSION     DASTKM010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554316"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..3537
                     /gene="ileS"
                     /locus_tag="VFQ10_04805"
     CDS             265..3537
                     /gene="ileS"
                     /locus_tag="VFQ10_04805"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET9964693.1"
                     /translation="MQAPVPTVERRSALGRELLYEEVSTMKFDKVSPQVDFPRLEEEI
                     LDLWKRTNAFERSVHGRPEDRPFVFYEGPPTANGRPGFHHALARAFKDLIPRYKTMQG
                     YRVERKGGWDCHGLPVEIQVEKELGLEGKADIERYGVEEFNRLCRESVFRYVEDWQKM
                     SERLGFWVDMEHPYRTLDASYIESVWWALKTLYENDLLYEGFKVSPHCPRDQTSLSSA
                     EVALGYQQYPDPVVDPSVYVRFPLRDEEHTSLLVWTTTPWTLVSNAAVAVGPDVEYAT
                     VEVDGERVILAEELLVKVLGEDGYRVVSKTKGRNLEGKRYRRPFDYVPVERAEDYENL
                     WTVVLGDYVTTTEGTGLVHTAPAYGEDDARTGREYGLPMIHPVRPDGTFDERVGPFAR
                     QFVKDADPGLVEELREKGLQFRAEVVEHAYPHCWRCGTPLLYYAKKAWYGRTTAVLDE
                     LLSENEKINWVPEHIKWGRFGDWLKNNIDWALSRERYWGTPLPIWRTTSGDTVVVGSK
                     RELKELAIDPVPDDLHRPYIDGVRVRHPETSEEATRVPEVLDVWFDSGSMPFAQWGYP
                     HGEASEERFRSQFPADYICEAVDQTRGWFYSLLAVSTMLFGKSSYRNCLVLGHILDAE
                     GKKMSKSLGNVIDPWEVFEQQGADALRWAFYTATSPGNTRRFSRGQVDEAVRKYLLTL
                     WNTYSFFVTYARIDGFDPKDDYVEPEGRSLMDRWALSELQLTVRTVTERLDAYDVTAA
                     GRALGEFVEELSNWYVRRSRRRFWKGEDDKDKKAAHSTLYECLVTVTRLTAPFTPFIA
                     EALYQNLVVNRDAEAPESVHLADWPEYREDLIDHDLSERMSAARRVVSLGRAARNAAA
                     IKTRQPLREVVVVDEAPDGMGGSRIKGGVESLKEIVLDELNVKELAFGKAEDVVAYDL
                     KPNLGVVGPRYGRLVPGLRTALAEAAPEVGARAAAGESISVGVEGEEITLSPEELLVE
                     PGQREGYALEREGGLSVALRTDLDAGLVDEGLVRELVHRVQNLRREKGFEIEESISVG
                     LSGNPRISSLLEGRWGDYFKAEVLAKELSLDAGAPGGGSERITVDGEALWVRVEPLGS
                     TG"
     gene            3605..4063
                     /locus_tag="VFQ10_04810"
     CDS             3605..4063
                     /locus_tag="VFQ10_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964694.1"
                     /translation="MFGRKSRAERLKEQAESNSLVPMATLAAAYAGAKPVIERLLYDD
                     DLRDNIRTFVESARSIVDQLSGEDPADIITKLWDDDKLRGQVEAAAGAASKGSKRIRG
                     EKVRERGGRGGRLLFLVLVAAVGFLFLHPRTGPRARKFAMDTYGSLTSGE"
     gene            complement(4103..4441)
                     /locus_tag="VFQ10_04815"
     CDS             complement(4103..4441)
                     /locus_tag="VFQ10_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964695.1"
                     /translation="MLGRLLGRRKKEPAPDLALISLAASAIREYAGKHAALFERAERL
                     REKADRLEKAGIPSESAANRAERARREVQAGLLALRASFASSVGGSAGERAFDHVIER
                     LYPTLGVQGQ"
BASE COUNT          915 a         1408 c         1555 g          746 t
ORIGIN      
        1 ctctctacag gcgatgacgg gaacgagtag ggtcgcagaa ccccctctga gcgagccggg
       61 gacggtggaa gcccggcgcg ggacgcggcc cgaatatcct cccagagccg ccggccgaaa
      121 gcggaaggcg agagtggcct agacggttag taggaccggc cgggcgagcc cgttacagcg
      181 cgtgcgttac gggagaccgc gacgccactg agaggaccgg agccgcgagg ttccggacaa
      241 gtagggtggt accgcgaggg cctcatgcag gctcccgtcc ctacggtgga gcgacgctcc
      301 gccctggggc gggagctttt gtatgaggag gtcagcacca tgaagttcga taaggtcagc
      361 ccgcaggtag attttccgag gctcgaggag gagatccttg atctctggaa gaggacgaac
      421 gctttcgaga ggtctgtgca cggcaggccc gaggacaggc ccttcgtgtt ctatgagggg
      481 ccgccgacgg ccaacggcag gccggggttc caccacgcgc tagcgagggc tttcaaggac
      541 ctcatcccca ggtacaagac catgcagggt tacagggtcg agcgcaaggg cggctgggac
      601 tgccacggtc tgcccgtaga gatccaggta gagaaagagc tcggcctcga aggcaaagcg
      661 gatatcgagc gctacggcgt cgaggagttc aaccgtctgt gccgcgagtc cgtcttccgt
      721 tacgtcgagg actggcagaa gatgagcgag cggctcggct tctgggtgga catggaacat
      781 ccttaccgca cgctcgatgc ctcatacatc gagagcgtct ggtgggccct gaagaccctc
      841 tacgagaatg acctcctcta cgagggtttc aaggttagcc cccactgccc ccgcgaccag
      901 acctccctct cctccgccga ggtcgccctc ggctaccaac agtaccctga ccccgtggtg
      961 gacccgagcg tctacgtgcg gtttcccctc cgggatgagg agcacacgag cctcctcgtc
     1021 tggaccacga caccgtggac cctcgtctcg aacgccgccg tcgccgttgg tcccgacgtg
     1081 gaatacgcca ccgtagaagt agatggcgag cgtgtgattc tggccgagga gctactcgtg
     1141 aaggtgctcg gggaggacgg ctacagagtg gtctccaaaa ccaaaggacg gaacctagaa
     1201 ggcaagaggt accgccgtcc cttcgattac gtacctgtgg agcgagccga agactacgag
     1261 aacctctgga ctgtcgttct cggggactac gtgacgacca cggaagggac cgggctcgtg
     1321 cacacggcgc cggcttacgg ggaggacgac gcccgcacgg ggcgggagta cggattgcca
     1381 atgatccacc ccgtcaggcc cgacggcacc ttcgacgagc gggtcgggcc tttcgccagg
     1441 cagttcgtca aggacgcgga cccgggtctc gtcgaggagt tgagggagaa gggactccag
     1501 ttcagggctg aggtggtgga gcacgcctac ccgcactgct ggcggtgcgg tacacctctg
     1561 ctgtactacg cgaagaaggc ctggtacggg cggaccaccg cggtgctcga cgagcttcta
     1621 tcggagaacg agaagataaa ctgggtcccc gagcacataa agtggggacg ctttggggac
     1681 tggctgaaga acaacataga ctgggccctg agccgcgagc gctactgggg cacgccgctc
     1741 ccgatctggc gcaccacttc cggcgatacg gtcgttgtcg gcagcaagcg agagctcaag
     1801 gagctcgcca tagaccccgt cccagatgac ctgcacaggc cttatataga cggggtacgc
     1861 gtgaggcacc ccgagacgag cgaggaggcc acccgcgtcc ccgaggtcct cgacgtgtgg
     1921 ttcgactcgg gcagcatgcc tttcgcccag tggggctatc cgcacggcga agccagcgag
     1981 gagcgtttcc ggagccagtt ccccgccgac tacatctgcg aggccgtcga ccagacgcgg
     2041 gggtggttct actcgctgct cgccgtctcg acgatgttgt tcggcaagag ctcgtacagg
     2101 aactgcctgg tcctggggca catcctcgac gccgagggca agaagatgag caaatctctc
     2161 ggcaacgtca tagacccctg ggaggtcttc gagcagcagg gtgccgatgc tcttcgctgg
     2221 gcgttctaca cggccacaag cccaggcaat acacgtcgct tctcaagagg tcaggtcgac
     2281 gaggccgtcc gcaagtatct cctcaccctc tggaatactt actctttctt cgtaacctac
     2341 gcccgcatcg atggcttcga tcccaaggac gactacgtcg agcccgaggg ccgaagcctg
     2401 atggaccggt gggctctaag tgaactgcag ctcacggtgc gtaccgtgac ggagcggctc
     2461 gacgcctacg acgtaacggc ggcaggaagg gccctcggag agttcgtgga agagctctcc
     2521 aactggtacg tgagacgcag ccgcaggcgc ttctggaagg gagaagacga taaagacaag
     2581 aaggccgccc actccaccct ctacgagtgc ctggtcaccg tcaccaggct cacagccccc
     2641 tttactccat tcatagcaga ggccctctac cagaacctgg tagtgaaccg agacgccgag
     2701 gccccggaga gtgtgcacct cgccgactgg cccgagtaca gggaggacct tatagaccac
     2761 gatctctcgg agcggatgtc cgcggcgagg cgcgtggtca gtctagggcg ggccgcccga
     2821 aacgccgccg ccataaagac ccgtcagcct ttgagggaag tggtcgtcgt cgacgaagcc
     2881 ccggatggca tgggaggatc gcggatcaaa ggaggcgtgg agagcctgaa ggagatcgtg
     2941 ctcgacgagc tgaacgtcaa ggagcttgcc ttcggaaagg ctgaggacgt ggtggcctac
     3001 gacctcaagc ccaacctcgg cgtcgtgggg cccaggtacg gcaggctcgt acccggcctc
     3061 aggacggccc tggctgaggc tgcgcccgag gtcggcgccc gcgccgccgc gggagagagc
     3121 atctccgtgg gtgtggaagg agaggagatc acactgtcac ccgaggagtt gctcgtcgag
     3181 ccaggacaga gggaaggtta cgccctggag cgcgaggggg gtctctccgt cgcactccgc
     3241 acagatctcg acgccgggct cgtagacgag ggtctcgtgc gggagctggt ccacagggtg
     3301 cagaacctga gacgcgagaa gggcttcgag atagaggaga gcatcagcgt cggcctctcc
     3361 ggaaacccca ggatctcgtc ccttctggaa ggtcgctggg gagattactt caaggccgaa
     3421 gtactggcaa aggagctctc cctcgacgcg ggcgcacctg gtggcggctc tgagaggata
     3481 acggtcgatg gggaggctct gtgggtcagg gtggagccgc ttggctcgac agggtagagg
     3541 cccggcgaaa cgggtatgat gtaggggtca caggcacaga cgattcagta gagggaggtt
     3601 tcgcgtgttt ggcagaaaga gccgggcgga gaggctaaag gagcaggcag agtccaattc
     3661 gctagtcccg atggcgacgc tggccgcggc ctatgccggc gccaagccgg tgatcgagcg
     3721 tctgctctac gacgacgacc tcagggataa catccgcacc tttgtcgagt cggcccgctc
     3781 catagtggac cagctctccg gcgaggatcc cgccgatatc ataaccaaac tgtgggacga
     3841 cgataagctc cgcggccaag tcgaggccgc ggcgggcgcc gccagcaagg gcagcaaacg
     3901 gatacggggc gagaaggtcc gcgagcgcgg tggacggggc ggtcgcctgc tattcctcgt
     3961 cctcgttgca gccgtaggct tcctgttcct ccacccgagg actggacccc gggcacgcaa
     4021 gttcgccatg gatacctacg ggtctctgac ctcaggagaa taggagcgga aaagaacgaa
     4081 cgcttcgcga ggagcgccgg ggttactggc cctggacgcc aagcgtcggg tacaggcgct
     4141 cgatcacatg gtcgaaggcc cgctctccag cactccctcc cacggatgag gcgaaggaag
     4201 cgcggagggc gaggagtccg gcttggacct ccctcctggc gcgctccgcg cggttggcgg
     4261 cgctctcgct cggaattcct gccttctcca accggtcggc tttctcccgc agccgctcag
     4321 cccgctcgaa gagggcggcg tgcttcccgg cgtactcccg gatggcggac gccgccagcg
     4381 agatcaaggc caggtccggc gccggctcct tttttcgacg gcccagaagc cggccgagca
     4441 cgcgcctggc acctccagga aaggaacgta gcctgagagc atccaagaga ggtagactgc
     4501 tttcactcat gaggtacatg ataaaccgcc tggcgcccgt cgttgtaatt ttgctctcgg
     4561 cattcgtgct cgccggatgc tcggccgtct tcggggagaa cccccgcgaa caggccgacc
     4621 aggc
//
LOCUS       DASTKM010000112         8151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1435680, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000112 DASTKM010000000
VERSION     DASTKM010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435680"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFQ10_04820"
     CDS             <1..319
                     /locus_tag="VFQ10_04820"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9964696.1"
                     /translation="VACSGDLLLGALAACAQVTCQMVAEAMGIETERIEVTVTGEIDL
                     AGTLGVSEDVPVGFETIRTSFDIVAPEATEEQLENLRQKTEQYCVVFQTLADSPDLQT
                     EWA"
     gene            335..1276
                     /gene="meaB"
                     /locus_tag="VFQ10_04825"
     CDS             335..1276
                     /gene="meaB"
                     /locus_tag="VFQ10_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET9964697.1"
                     /translation="MAKKGLVERLLAGDRRALARVISKIEREDEETAGIIAEIYPETG
                     RALTVGFTGPPGVGKSSIIAKLIELYRQEERRVGVVTVDPSSPFSKGAILGDRIRLSD
                     HFLDPGVFIRSMGSRGHLGGLAGGSRLAAIAMDAGGCDVVIYETVGVGQGEVEVASAA
                     DTVVLCLQPGTGDSVQALKAGVMEIADVFCINKADNPQAKNAASEVRSILEIGHELDP
                     DPWFPPIVMTRGDTGEGVEELKDTIAKHRQFLEEDGRLGKRRRAALRDFVVSWATNRL
                     EKEMHERLAREDTELVEKVYNRELDPISASERIFKEV"
     gene            complement(1273..1761)
                     /locus_tag="VFQ10_04830"
     CDS             complement(1273..1761)
                     /locus_tag="VFQ10_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HET9964698.1"
                     /translation="MRARAASSEDAEAIARIYNEGIEERIATFETSPRAAREIQAWFD
                     GTHPVVVVEEEGEVISFANTTAYSPRGCYSGVAEFSVYTASEARSRRAGTLAMRALME
                     AAEEAGFWKLVARVFVENEPSRKLLLSLGFREVGVHEKHARLDGVWRDVVVVERLLPA
                     NL"
     gene            complement(1851..2210)
                     /locus_tag="VFQ10_04835"
     CDS             complement(1851..2210)
                     /locus_tag="VFQ10_04835"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET9964699.1"
                     /translation="MTDLRALAGEDFCYLTTTGRVTGRPHEIEIWFALVPGAHTLYML
                     SGGGNRSDWVKNLRRYPDVEVRIAGERLGGRAREAKDAEEDELARRLLVEKYESSPGR
                     LESWRRRALPVVVDLAK"
     gene            complement(2282..3874)
                     /locus_tag="VFQ10_04840"
     CDS             complement(2282..3874)
                     /locus_tag="VFQ10_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9964700.1"
                     /translation="MANIGDYERTYADFDWETPQRFNFGRDVVDRWAAQDRAAMVWLG
                     TDGEERRLDFAQFSVLSNSFANAARALGIERGDRVMVLLGKVPEWHVILTGLLKLGAI
                     AIPCAPQLRAGDLKFRAGHSGSVAMISGPEGIEEVEKMRQDVPGLEHFISLGGERDGW
                     ESYETLMEGPSEDFTAEDTTADEGAFILYTSGTTKHPKGALHTHGYTYAKWMEARYWL
                     DLREGDRLWCTSGTGWAKSIWNVFLGPWSQGTEIFFHEGGFDPAERLELMELYDISVL
                     CQAPTEYRLLAKTPELERANLASIRHAVSAGEPLNPPVIERWKDLHGLTIYDGYGQTE
                     NTLLVGNYPGLDLRPGSMGKPSPGCDVKIVDPEGNECQPDEPGDIALFGRIPALFEEY
                     WEQPDATEAVFRDGHYLTGDRAYRDADGYLWFVGRSDDVILSAGYRIGPFEVESILIE
                     HPAVVESAVVPLPDEDRGSVVKAFIVVGEDYQPSEDLAKEIQAYCKENTAPYKYPRSI
                     AFIDDLPKTTSGKIRRVELREE"
     gene            complement(3965..5296)
                     /locus_tag="VFQ10_04845"
     CDS             complement(3965..5296)
                     /locus_tag="VFQ10_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9964701.1"
                     /translation="MRVVIAGGGLAGLTATLRARELGAHATLLEKGACPGGSFVYSSG
                     YIWSYVDLPTFRREAPGGDAILQRLIFERLGSCLTWLEVAGGDVIARETGNPLTFGAR
                     FDPKRTVSALVDRIETSGGEILADTALEELLEDTTGRVTGVLARSSEGKKEYDADAVI
                     LASGGFAGDPELVEHHIIGGPGRMRMRAHPRSTGDGFRAALQKGALASAGLDEFYGRN
                     LPAPPADFPPERFVEVSQLYGRYAVAVNASGERYADEGADWSETALTRATAHQPGLYA
                     WYVLDARGLEGRVRERTAKEMVETARSTGGTVIEAASLTELADRLAERGVPRETFLRT
                     LEEYNAAAAAGGDLSPPRSGHPWPLRVPPFVAVKVAPSITHTVGGLAVDTGCRVLRGT
                     DGRPIPGLYAAGVEVGGVSVGGYTSGLASALVFGGTAAESAVADMQELDNL"
     gene            complement(5307..6002)
                     /locus_tag="VFQ10_04850"
     CDS             complement(5307..6002)
                     /locus_tag="VFQ10_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9964702.1"
                     /translation="MRVKRLDHIALYMGDRDAATGFLTTHLGFHVVDHTERYTLVGAG
                     GRLGKLTLFDAPQGKTPSPGEIERITIRVADPEAAAARLPREAGPEPRDGGYSFTGPE
                     GLPLALVSGEGEFADYDLDGFVLRSGSPEESARTFVELGFAPGDGATTVKAGDYLIRL
                     TGSSPDANGGGMLFHVGCLVDSAEDHRKEAEERGLEVQDFVDGPNTLAVFIRGPEGVS
                     VEYVEHKSTFSLT"
     gene            6315..7490
                     /locus_tag="VFQ10_04855"
     CDS             6315..7490
                     /locus_tag="VFQ10_04855"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET9964703.1"
                     /translation="MERTVILGAARTPFGRFGGALSPLGAPELGGAAIRDAIDRSGIE
                     DGDLQHSIFGIVVQAGVGQIPSRQANFHAGLTFSLTTETLNQVCASGLRSATLAETLI
                     RAGDYDVIVAGGMESMSNAPYLLPKARWGARMGDMPTLDAMMHDGLLDAFEHVNMIRF
                     GTRGARKFDISRQEQDAWALRSHKRAAAATDEGRLPEEIVGVKVSGKKGKTTYVEADE
                     PIRRDTTMEKLAALEPIDEGGTVTAGNAPGVTDGAGALVLAGKSFAERRGLEPLGSIV
                     AHAKVAEEPPNLLTVPGNAGKLALEKAGWNADDLDLVEINEAFAGVAIHSTRILGVDP
                     DIVNVNGGALALGHPVGASGARILTTLLYELRRRGGGKGLAAICSGGGQGDAVLVEV"
     gene            7621..>8151
                     /locus_tag="VFQ10_04860"
     CDS             7621..>8151
                     /locus_tag="VFQ10_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET9964704.1"
                     /translation="MDLNERGNVFPHGDTFPQVARSAWVAPGAWVIGDVNLGEESSVW
                     YGAVLRGDTEPIRIGARTNVQDGCILHADPGYPAVVGEGCVLGHNAVVHGCEIEDHCL
                     VGMGCTILNGAKIGEGSIVAAGAVVPEGREFPPHSLIVGIPAKRAGEVSEEQAADIAR
                     GASEYIERAKEHRESLG"
BASE COUNT         1464 a         2634 c         2601 g         1452 t
ORIGIN      
        1 ggtcgcttgt tcaggagacc ttctgctggg agcgttggca gcctgtgccc aggtaacctg
       61 ccagatggtc gccgaggcca tggggataga gaccgagcgc atcgaggtca ccgtgacggg
      121 ggagatagat ctcgccggaa cgctcggcgt ctccgaggac gtccccgtcg gcttcgagac
      181 tatccgcacc agcttcgaca tcgtggcgcc tgaggctacc gaagaacagt tggagaacct
      241 gcgccagaag acagagcagt actgcgtcgt cttccagacg ctggccgact cgccggacct
      301 ccagacggag tgggcgtaga agatcggacc cagcttggct aagaaagggc tcgtagagcg
      361 gttgctcgcg ggcgaccgtc gggcgctggc gcgggtgatc tcgaagatag aacgtgaaga
      421 cgaagagacg gccgggataa tcgcagagat ctaccccgag accggtagag cccttacggt
      481 cggtttcaca gggccgccag gcgtcggcaa gagcagcatc atcgcgaagc tcatagagct
      541 ctacaggcag gaagagaggc gggtcggggt ggtcactgtc gacccttcga gcccgttctc
      601 gaagggtgcg atccttggcg accgcataag gctctcggac cacttcctcg accccggcgt
      661 tttcatccgc tcgatgggta gcaggggaca cttgggcggg ctcgccggcg gctcgcggct
      721 cgccgccatt gcgatggatg cgggcggctg cgacgtggtg atctacgaga ccgtcggggt
      781 tgggcaagga gaggtcgagg tcgcctcggc tgcagatacg gtggtgcttt gcctgcagcc
      841 gggaaccggc gactcggtgc aggcgctcaa ggccggggtg atggagatag cggacgtctt
      901 ctgcatcaac aaagcggata acccgcaggc gaagaacgcc gcttcagagg tgcgttccat
      961 actggagatc ggacacgagc tcgaccccga cccatggttc ccaccgatcg tgatgacccg
     1021 cggcgataca ggcgagggcg tcgaggaact gaaagacacc atagcgaagc accgtcaatt
     1081 cctcgaggag gacggcaggc tcggcaaaag gcggcgggcc gccctgagag acttcgtcgt
     1141 ctcgtgggcc acgaaccgcc tggagaaaga gatgcacgag cgcctggccc gcgaggatac
     1201 tgagctcgtc gagaaggtct acaacaggga actcgacccc atctcggcct cggagcgcat
     1261 cttcaaggaa gtctagagat tggccggaag aagtcgctcg acgactacga cgtcgcgcca
     1321 tactccgtcg agcctggcgt gtttctcatg gactcccacc tcccggaagc ccaaagagag
     1381 taggagcttc cggctcggct cgttctccac gaagacacgc gcgacgagtt tccagaagcc
     1441 ggcctcctcc gctgcttcca tgagcgccct catcgccagg gtgcctgctc tgcggctcct
     1501 ggcctcgctg gcggtgtata cggagaattc ggccacgccg gagtagcacc cgcgggggct
     1561 gtaggccgtg gtgttggcaa aggagatcac ctccccttct tcctccacca ccacgacagg
     1621 gtgagtccca tcgaaccagg cctgtatctc ccgtgccgct cgggggctgg tctcgaacgt
     1681 cgcgatgcgc tcctcgatgc cctcgttgta gatgcgggct atggcttctg cgtcttccga
     1741 actggctgct ctggcccgca cgaccctatc ctcacctcga ttcttccaga tagccccaac
     1801 atgccctcct gccgcagtcc tcgctctttg gcgccacccc cgcgtagccc ctacttcgcg
     1861 aggtccacca ccacgggcag cgctctgcgg cgccagctct ccaggcgacc cggactgctc
     1921 tcgtacttct cgaccagcaa cctccgcgcc agctcgtcct cttcggcgtc tttggcctcg
     1981 cgggcgcgac cgccaagacg ctcccccgct atgcggacct ccacatcagg atagcggcgg
     2041 aggttcttca cccagtccga gcggttacct cctcccgaga gcatgtagag ggtatgtgct
     2101 ccgggtacga gggcgaacca gatctcgatc tcgtgcggcc tccccgttac cctccccgtc
     2161 gtcgtcaggt agcagaagtc ctcgcccgcg agcgcacgca ggtcagtcac agaccccagg
     2221 cttcaggcct gcatggcagt gttgcaagaa acctttccct gacatctgat acctgtggcc
     2281 ttcattcctc tcgcagttcg acgcgacgga tcttgccgct cgtcgtcttg gggaggtcgt
     2341 cgatgaacgc tatgctgcgc gggtacttgt acggggctgt gttctccttg cagtaggctt
     2401 ggatctcctt cgccagatcc tccgaaggct ggtagtcttc gccaacgacg ataaacgcct
     2461 tgaccacgct tcccctgtcc tcgtcgggca agggtacgac ggcgctctcg acgaccgcgg
     2521 gatgctcgat gaggatgctc tcgacttcga agggcccgat gcgataaccg gcagagagga
     2581 tgacgtcatc cgaacggcca acgaaccaga ggtagccgtc cgcgtctctg taagccctgt
     2641 caccggtcag gtagtggccg tctcggaaga cagcctccgt cgcgtcgggc tgttcccaat
     2701 actcttcgaa cagcgcgggt atgcgcccga agagggctat gtcgccaggc tcgtcaggct
     2761 ggcactcgtt accttcgggg tcgacgatct tcacgtcgca gccaggagac ggtttgccca
     2821 tcgagcccgg tcgtaagtcg aggccgggat agttgccgac gagcagggtg ttctcggtct
     2881 gcccgtaacc gtcgtagatg gtcaggccgt gaaggtcttt ccagcgctcg ataacgggcg
     2941 ggttcagcgg ttctccggcg gagacggcgt ggcgaatgga ggcaaggttc gcgcgttcca
     3001 gttccggggt cttggcgagg agccggtact cggtcggggc ctggcagagg accgaaatgt
     3061 cgtagagctc catcagctcc aagcgttcgg cagggtcgaa accgccctcg tggaagaaga
     3121 tctccgtccc ctggctccac ggccccagaa atacgttcca gatgctcttg gcccagcccg
     3181 ttcccgaggt gcaccacagc ctgtcgccct ccctgaggtc gagccagtac ctggcctcca
     3241 tccacttggc atacgtgtag ccgtgggtgt gcagagcgcc tttgggatgc ttcgtcgtac
     3301 ccgaagtgta gaggatgaac gcaccctcat cggccgtagt gtcctcagcc gtaaagtcct
     3361 cagaaggccc ttccatcaac gtctcgtagg attcccaccc gtcgcgctct ccgcccagag
     3421 agatgaagtg ttctagacct gggacgtctt ggcgcatctt ctcgacctct tcgatgcctt
     3481 cagggcccga gatcatggcc acagatcccg agtgcccggc cctgaacttc aggtcgccgg
     3541 ccctgagctg aggggcgcac gggatggcaa tagcccctag cttcaagagg cccgtcagga
     3601 tcacgtgcca ctcgggcacc ttgccaagta ggaccatcac cctgtcgcca cgctctatac
     3661 caagtgctct cgccgcgttg gcgaagctgt tcgagaggac ggaaaactgg gcgaagtcga
     3721 ggcggcgttc ctcgccgtcc gttccgagcc agaccatggc cgcacggtcc tgcgcggccc
     3781 accggtccac cacgtcacgc ccgaagttga acctctgcgg cgtctcccag tcgaagtctg
     3841 cgtaggttct ctcgtagtcg cctatattcg ccaaggtcca tcacctttcc ccaaaggtcg
     3901 ctttccggaa ccgcattcta ctagaaccag tatctctggc acgcgcggag taccacccga
     3961 cgggctagag gttatctagc tcctgcatat ctgcgaccgc cgactccgct gcggtgccac
     4021 caaagacgag ggccgaggcg aggccgctcg tgtacccgcc gacggagacc ccgccgacct
     4081 cgacccccgc ggcgtagagg cctgggatgg gccgtccgtc cgtccctcgc agcaccctgc
     4141 agcccgtgtc cacggcgagc ccgcctaccg tgtgcgttat ggatggcgcg acctttacag
     4201 ccacaaaggg aggaacgcgc aagggccagg ggtgtccact cctcggggga gaaagatctc
     4261 cacccgcggc ggcggcagcg ttgtactcct ccagggtgcg gaggaaggtc tcgcggggca
     4321 cgccacgttc agcgagtctg tccgcaagct ccgtgaggct tgcagcttcg atgacggttc
     4381 cgcccgtgct ccttgccgtc tcgaccatct ctttcgcggt ccgctcgcgc acgcggcctt
     4441 cgagccctct ggcgtcgaga acataccagg cgtagagtcc gggctggtgt gccgtggccc
     4501 ttgtcagggc cgtctcagac cagtccgcgc cctcgtcagc gtagcgttcg ccgctggcgt
     4561 tgacagccac ggcgtagcgg ccgtagagct gagagacctc cacgaatctt tcaggaggaa
     4621 aatcggccgg gggagctggc aggttgcggc cgtagaactc gtcgagcccg gcacttgcga
     4681 gtgcgccctt ctgcagcgcc gcccgaaaac cgtcgccagt actccttggg tgcgcccgca
     4741 tccgcatcct tccaggtccc ccgattatgt ggtgctcgac cagctcgggg tcgccggcga
     4801 agcccccgga ggcaaggatg acggcgtcag catcgtactc cttcttgccc tcggaggatc
     4861 gggccagcac gccggttaca cgtcccgtgg tgtcctccag tagctcctct aacgcagtgt
     4921 ctgcaagtat ctcgcctcct gaggtctcta tacggtcaac cagcgccgag accgttcgtt
     4981 tggggtcgaa gcgggcgccg aacgtgaggg ggttccccgt ctcccgcgcg atgacatcgc
     5041 ctcctgcgac ctccagccaa gtcaggcagg acccgagccg ttcgaaaatg agcctctgga
     5101 gaatagcgtc cccgcctggc gcctccctgc ggaaggtcgg cagatccaca tacgaccaga
     5161 tatagccgct ggagtagacg aaggacccgc cgggacaggc tcccttctcc agcagggttg
     5221 cgtgcgcgcc aagttccctg gccctcaggg tagcggtcag gccagcgagc ccgccgccgg
     5281 cgatgacgac gcgcaccggt gctactctag gtcagggaga acgtggactt gtgctccacg
     5341 tattccacgc tcacgccctc gggccctcgg atgaagacag cgagggtatt cggaccatcg
     5401 acgaagtcct ggacctccag cccccgctcc tcggcctcct tgcggtgatc ctccgccgag
     5461 tctacaaggc aaccgacgtg gaagagcatg cctcctccgt tcgcatccgg cgaggagccg
     5521 gtgagtcgta tcaggtagtc gcccgccttc acggtggtag ccccgtctcc cggggcgaag
     5581 cccaactcga cgaaagtgcg cgcggactcc tcgggcgatc ccgatcgcag gacgaagcca
     5641 tcgaggtcgt agtccgcgaa ctctccttcc ccagaaacca gggcgagcgg aagcccttca
     5701 gggcctgtga aagaataccc tccgtcgcgg ggttcaggcc cggcctctcg cgggagtctc
     5761 gctgcggcgg cctccgggtc ggccacgcgt atggttatgc gttcgatctc ccccggagag
     5821 ggtgttttgc cctgcggggc gtcgaacagg gtgagcttgc cgagcctgcc accggcccct
     5881 accagagtgt aacgttccgt gtggtccacg acgtggaacc cgaggtgcgt tgtcaagaac
     5941 ccggtggcag catccctatc gcccatgtag agtgctatgt gatccaacct ctttactctc
     6001 atggattctc cttgtcatgc cactatcttc cgagaagata gcatgtgata ggtaatctgg
     6061 tatttcactt ctggttgaat gtacgcagcg ccagccgctg tggattcccg cctgcgcggg
     6121 aacgacggtc tcatggtgcg agcaagggag taggagttgc gctcgtcttg gttgtacacc
     6181 tgggaagtga tcctgcgggt tccgtacgac gtcgtctcgg tcgcaacctt tcggcggctg
     6241 ggcctgccga aacgcgcgtt tcatcccagg aaggttagac tatggtcgtt ggtcttcgag
     6301 acagaggagt cccattggaa cgtacggtaa tcctcggggc ggcgcggacg cccttcggaa
     6361 ggttcggcgg cgcattgtcc ccccttggag cccccgaact cggtggggct gcgatcaggg
     6421 acgccataga ccgctccggt atcgaagacg gagacctgca gcactccatc ttcggtatcg
     6481 tggtgcaggc cggggtaggg cagatcccat cccgccaggc taacttccac gcaggcctca
     6541 ctttctcgct aacgacagag accctgaacc aggtatgcgc ctctgggctg aggagcgcga
     6601 cgctagctga gaccctgatc cgtgccggcg actacgacgt gatcgtggcc ggcggaatgg
     6661 agagcatgtc gaacgcacct tacctcttgc cgaaagcccg ttggggtgcg cgcatggggg
     6721 acatgcccac gctcgacgcc atgatgcacg acggtctgct cgacgccttc gagcacgtga
     6781 acatgatccg cttcggcaca cgaggcgcga ggaagttcga tatctcacgc caagagcagg
     6841 acgcgtgggc gctcaggagc cacaagcgcg ccgccgcagc gaccgacgaa gggcggctgc
     6901 ccgaagagat agtcggcgtc aaagtctctg gcaagaaggg gaagacgacc tacgtggagg
     6961 cggacgagcc gattcggcgc gacacaacca tggagaagct cgccgccctc gagcccatag
     7021 acgagggcgg caccgtgacc gccggcaacg cccctggcgt cacagacgga gccggcgccc
     7081 tcgtgctcgc gggcaagagc ttcgccgaaa ggcgcggcct cgaaccgctc ggttccatcg
     7141 tggcgcacgc caaagtagcc gaggagccgc ccaacctgtt gaccgtgcct ggcaacgcgg
     7201 gcaagctggc gctcgagaag gcaggctgga acgcggacga cctggacctg gtcgagataa
     7261 acgaggcctt tgccggcgta gcgatccact ccacacgtat actcggcgtc gaccccgaca
     7321 tcgtcaacgt caacggcggt gccctggcgc taggccatcc cgtgggcgcg tcaggcgccc
     7381 gcatcctgac gacgctcctc tacgagctgc ggcgcagagg aggcggaaaa ggtcttgcgg
     7441 ccatctgctc cggcggtggc cagggcgacg ccgtgctggt ggaagtatga gcgacggtgt
     7501 gggggcggtt cgcgaaccgc ccccacaacg cagagcgctc cggcgaaggc gtgacggcaa
     7561 ccgaaaggag ctgtggagat ttggcgacga gtacattgag cacaaggtaa gggagcggcg
     7621 ttggatctga atgaaagggg caatgtgttc ccgcacggcg ataccttccc gcaggtcgcg
     7681 cggtctgcct gggtggcgcc tggagcctgg gtcatcggcg acgtcaacct gggcgaagag
     7741 tcgagcgtct ggtacggggc cgtgttgcgc ggcgacacgg agcccatccg tatcggtgcc
     7801 aggaccaacg tgcaggacgg ctgcatcctc cacgccgacc ccggctaccc cgcggtcgtt
     7861 ggcgagggat gcgtcctcgg ccacaacgcg gtcgtgcacg ggtgcgagat cgaggaccat
     7921 tgtctcgtag ggatggggtg taccatcctc aacggcgcga agatcggtga gggctccatc
     7981 gtcgccgccg gcgccgtcgt ccccgagggt cgcgagttcc cgccccacag cctcatcgtc
     8041 ggcattcccg ctaaacgcgc cggcgaagtg agcgaggagc aggcagcgga catagcccgc
     8101 ggcgcgagcg agtacataga gcgcgccaaa gagcaccggg agtcgttggg a
//
LOCUS       DASTKM010000113        12415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_731273, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000113 DASTKM010000000
VERSION     DASTKM010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12415
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731273"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VFQ10_04865"
     rRNA            <1..422
                     /locus_tag="VFQ10_04865"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            496..612
                     /gene="rrf"
                     /locus_tag="VFQ10_04870"
     rRNA            496..612
                     /gene="rrf"
                     /locus_tag="VFQ10_04870"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            676..2034
                     /locus_tag="VFQ10_04875"
     CDS             676..2034
                     /locus_tag="VFQ10_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9964705.1"
                     /translation="MFELSGDTMVVFVSDSHIGGDPGCDGFESPKELEALFVELAGRE
                     GPVELILAGDFFDFLQIGEVAPGKNRASLTIGRPEYRDLFAALERFREAAGKRVIYLP
                     GNHDAESFWNKEIQETLRERGLVDEFAYYYLASVETGGGRRVVYCEHGNQFDPENRVG
                     DYHDPLDTPVGHHVVMDGTRRIAPFGEISSGLDLSEIKMVYPLVAIPAWIASRYFYNM
                     AGKVASYLLLPLLVAYMVYKVVAFAIARATDGKASLLFGSYRELPHVHQAFLDTVLFL
                     LVVFGIFGVFFLVVRHAVHRTLRAVSPGGTPHYSPAEASQKRIRAVLAGEARPPMDPA
                     FDPTTADVFVSGHTHLPSLAEMERPDGNKAVLVNSGCFLRQLQPITPRLKGPPVFVSK
                     FVLTHVRVFAQGEKLRVELWQQPKPAGQTLTRIERLVSIGRRPPQPPAGAKPRLVASA
                     TV"
     gene            2125..2511
                     /locus_tag="VFQ10_04880"
     CDS             2125..2511
                     /locus_tag="VFQ10_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964706.1"
                     /translation="MADQELDQQFVESQRERLVGLRDELVRIREGMADDERDLGEAEG
                     DTQLDSGDLSQDMFTREMDASIGEQVGRRLGEVDRALQKIEEGTYGLSDDSGESIPRG
                     RLEAVPEALRTVEEQQRFERERRPPI"
     gene            2537..3406
                     /locus_tag="VFQ10_04885"
     CDS             2537..3406
                     /locus_tag="VFQ10_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9964707.1"
                     /translation="MDHRSLGDDTREPIFRSDLEEPRPEPTYEPLRPEGGLWSVIKRW
                     VAPLAAVGLLLAKFKGLALLLLKVKFLGTALTMLVSIGAYALLFPVWFAVGIVVLIWV
                     HEMGHVLQLRREGIPASAPMFIPFLGAFVAMKQMPKDALAEARVGLAGPVLGTLGGLA
                     TLGLYAATREPLFLGLAYFNFIINLFNLAPLLPLDGGRAVGAMSPVFQLLGLVVMVAL
                     FFVAPIMAFIAVLGLPELWHRWKTRNTLEGRAYYDLPLRQRVAVGLVYLGLIAVLGLL
                     TGATFVPDPAALQ"
     gene            3408..4577
                     /locus_tag="VFQ10_04890"
     CDS             3408..4577
                     /locus_tag="VFQ10_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HET9964708.1"
                     /translation="MRTILYTGKGGVGKTSVAAATALKASLAGKKVLVMSTDPAHSLA
                     DVFDTEIGQDPKEMAPGLWAQEMDHTSMIEDSWAEIQGYMTTLFEWQGADTLAAEELA
                     MLPGMDELFGLLMVRRHNQEDRYDALILDAAPTGETLKLLSLPDHMSWYVEKIFPIQR
                     RAAKLVRPFANRTRSLPPLPQDSVFAAGQRFYEAIASVEEILTDRESASVRLVLNAEK
                     MVIAEARRAYTYLNLYDYGVDALVVNRLLPETVSDPYFARWHDVQARHMRTIEEAFDP
                     IPILTARLFDREMFGLEALGALAEDVFEDAEPLGVLFKGATHDIEKNGDGYDVVFHLP
                     LAEKRNVDLSKKGAELLVKVGNYRRNILLPDALARLPAAGASFEGGSLKVRLRDG"
     gene            4570..4773
                     /locus_tag="VFQ10_04895"
     CDS             4570..4773
                     /locus_tag="VFQ10_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964709.1"
                     /translation="MAEEKRHRGKLLGVIAYVLVPGERRVRAAAHFRKAGFEALKGVG
                     ELVRPEKSQTSTGPTSRERIEIE"
     gene            4806..5009
                     /locus_tag="VFQ10_04900"
     CDS             4806..5009
                     /locus_tag="VFQ10_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964710.1"
                     /translation="MRGQELLQRVGEFARKEVTLALPAAQRAASHGIQAFLKKFSEEY
                     AIERNKLKSGASTPSRFGHQGRR"
     gene            5055..5951
                     /gene="pdxS"
                     /locus_tag="VFQ10_04905"
     CDS             5055..5951
                     /gene="pdxS"
                     /locus_tag="VFQ10_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET9964711.1"
                     /translation="MVENGQLTGTFRVKSGMAQMLKGGVIMDVVDAEQATIAEEAGAV
                     AVMALERVPADIRADGGVARMSDPEKIVEIQESVTIPVMAKARIGHFVEAQILEALEV
                     DYIDESEVLTPADEKHHIDKAAFEVPFVCGATDLGEALRRIGEGAAMIRSKGEAGTGN
                     VVEAVRHMRAIVGGIRRLGALEHEEMMTEAKNLGAPYELVRWVAENGRLPVVLFTAGG
                     IATPADAALMMQLGADGVFVGSGIFKSADPALRARAIVKATTHFKDAKAVAEASRGLG
                     VAMVGREMGDLAEGERLATRGW"
     gene            5941..6540
                     /gene="pdxT"
                     /locus_tag="VFQ10_04910"
     CDS             5941..6540
                     /gene="pdxT"
                     /locus_tag="VFQ10_04910"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET9964712.1"
                     /translation="MAGNGETNGPKKIGVLALQGDFREHAEILRGLGVEPVEVRAVED
                     LEGLAGIIVPGGESTTIGKMMASSGLLDGIRSFFYKGGPVWGTCAGMVLAASATTGPR
                     QPLLGLMNALVERNGFGRQVHSFEKDLEVEGFDEPFTGIFIRAPFFEDVGPGVEVLSR
                     IGDRVVAARGENILVTAFHPELTDDARFHEYFLWEVCSI"
     gene            6537..7289
                     /locus_tag="VFQ10_04915"
     CDS             6537..7289
                     /locus_tag="VFQ10_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9964713.1"
                     /translation="MSGHSKWSTIKRKKGAADAKRGALFGKLSRAISVAAREGGGDPE
                     MNAPLGLAIQKAKDANMPNDNIQRAIDKGTGAGADDDTYERITYEGYAPGGVAVLVDV
                     LTDNRNRAASDVRYVFSKNGGKLGTSGSVAYLFERKGVILVPRDSTDEDTLMEVALEA
                     GAEDVEEQENDYRVVTTPEDFTAVREALGEADIPYENAEITMEPQNSIDLDAGTARQT
                     LRLIDALEENDDVQEVYANFDISEEVMAEVVS"
     gene            7358..8512
                     /locus_tag="VFQ10_04920"
     CDS             7358..8512
                     /locus_tag="VFQ10_04920"
                     /inference="COORDINATES: protein motif:HMM:NF017691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HET9964714.1"
                     /translation="MALLVALLVSSACSSGDEGKGGDEDVIAGASKQRTMDLPPKQTE
                     TDGGIGDGVETGNLRFRIFEVRAKDRVYAMSGPGKRPVTRGNISSEYVAIDYLAKNLS
                     GSPLTTGAKATLLDDQGNSYGQDKSIEPPSGGTDGMELGTAQTKASTMFFEVPNGVIP
                     ATLEIEASGGEASFDLLQRNMERVPPDDYLRVYHLYFNEQAYEEAYEMFDPASVQNIT
                     LGEWLTFYEPLWGKRYVSLDGLTPLSEDSDSATFQMRRTFYDADGDIVADPEINPSVI
                     QEMVEVDGEWKLVMRDDLVSDIIAVIGPDETPRPKAPEPEKKGPERTQPPSTVPETTE
                     PSEATRSAAPSRDYDCDDFKNQEEAQLYLAPGDPYGLDEDGNGVACETLP"
     gene            complement(8581..9672)
                     /locus_tag="VFQ10_04925"
     CDS             complement(8581..9672)
                     /locus_tag="VFQ10_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9964715.1"
                     /translation="MSSSGNRVTLTSIARKLGVSAMTVSNAYNHPDRLSEALRERIFE
                     TTGRLGFHGPDPVGRSLRRQRTDEVGVLYSNPLSYAFDDPAAVSFLGGLSSVTEEADL
                     GLLLVPAAGGGESSERDPRAAAQAAVDGFVIYSMSDEEPLLAAALQRRLPVVVVDQPR
                     KEGVPFVGVDDEGAARMAAEHLLDLGHERFAIVSFGLAPDGREGIADSDRQEGTAFHV
                     SRMRLRGYRAAMEEAGLPWSEVPVYECQGSAKELGYWAIEALFRSEQEPTAILALSDQ
                     LALGAIEWMTEWRISVPQDVSVVGFDDIPAAASADPPLTAIHQDHAEKGTMAGRMLVA
                     QLHDEDAPSAEPLATHLVVRGSTSPNRKR"
     gene            9762..11180
                     /locus_tag="VFQ10_04930"
     CDS             9762..11180
                     /locus_tag="VFQ10_04930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9964716.1"
                     /translation="MDVGGHTRVERKDAALILLCVAQFVDVLDVNAVIVALHSIGQDL
                     GFSQAGLQWVVSAYVLFLAGFLLLAGRVADLWGRRRTFVVGLAVFTAASLCCGLSVSK
                     EMMVVSRAFQGLGAATTAPAALSIITTIFAEGRERDRAVAAWTAVAAGGGAAGLLLGG
                     FITDVLGWQWIFFVNVPVGAAGIALSQVLLPECRDPAASRRLDLLGAGTVTSGLVLLV
                     LALTRTEEAGFGSPITLATLGFAAALFGAFVFVERRAADPLVPLRLFRLRGLVGAALV
                     AFAITAATAPVGVLVTLYLQNTLGYPASYAGLAGLPFSLCVIAGSMLGGRISGRIGGR
                     VTMSLGLAVVAVSALLTAGISAEGGVAYVLAGAALSGLGLGCASVASTARGTSAVEEG
                     KRGLASGFMNTSAQVGTALGLAALLTLAATRTAALYDGAESAAEALVAGYRLAFLVAA
                     GVASLGVVAALYFFRQAKDFDT"
     gene            11299..11820
                     /gene="ruvC"
                     /locus_tag="VFQ10_04935"
     CDS             11299..11820
                     /gene="ruvC"
                     /locus_tag="VFQ10_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET9964717.1"
                     /translation="MSPTGGIGHTILGIDPGTATMGWGVIRREGNRLRYIQHGAIITP
                     PDWEMPRRLGRLFDGVTELVRGYRPETIAVEELFFNTNVTTAITVGQARGVALLAAYK
                     AGIEVTEYTPLQVKQAITSYGRADKRQMQEMVKALLNLPAIPRPDDAADGLAIAICHA
                     FSSTMPGKVGVGK"
     gene            11845..>12415
                     /gene="ruvA"
                     /locus_tag="VFQ10_04940"
     CDS             11845..>12415
                     /gene="ruvA"
                     /locus_tag="VFQ10_04940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET9964718.1"
                     /translation="MIYRLRGTLVEKDTEGVVIDVGGVGYRTSASLATLRALPSLGEE
                     CVIHTRMVVRDDAILLFGFAVREERAAFDALTAVSKVGPKLALSILSSMSPPEISETV
                     ARGDVIKFASVPGLGKKTAERLVLELKGKSLAAFGPEPVVAGGGGGGPYMEARDALTA
                     LGYGLEEAEKALNDVPPQDSVEKYIKEALR"
BASE COUNT         2317 a         3630 c         4179 g         2289 t
ORIGIN      
        1 cgacgcggct gtttggcacc tcgatgtcgg ctcgtcgcat cctggggctg gagtaggtcc
       61 caagggttgg gctgttcgcc cattaaagcg gtacgcgagc tgggttcaga acgtcgtgag
      121 acagttcggt ccctatcttc cgtgggcgtt ggagatttga gaggagcgac tcctagtacg
      181 agaggaccgg agtggacgta cccctggtgt accggttgtc ctgccaaggg caccgccggg
      241 tagctatgta cggaagggat aaccgctgaa agcatctaag cgggaagccc gcctcgagat
      301 gagatctccc atccccatag agggagtaag actcctggta gaccaccagg ttgataggcc
      361 acaggtgtaa gtgcagcgat gtatgtagcc gagtggtact aataagtcga gggcttgatg
      421 tactctttac ggttcgcgct gtgcggtttt caaagggcag gcgcccttta caactcaata
      481 cttgccgctt tttagaaggg tgacgatggc ggcggggata cacctcttcc cattccgaac
      541 agagaagtta agcccgccag cgccgatggt actgcggggg agaccccgtg ggagagtagg
      601 tcgtcgccca ttttttgttg ccccaataat ggggtctggt tgtcagagag cctgcgtttg
      661 ggctaggctt ggtgcgtgtt cgagctgagc ggcgatacca tggtggtctt tgtctctgat
      721 tcgcacatcg gaggggaccc tggttgcgac gggttcgagt cgcctaaaga gctggaggct
      781 ctattcgtgg agcttgctgg cagggagggg cctgtcgagt tgatcctggc gggggacttc
      841 ttcgatttct tgcagatagg cgaggttgcg cctggaaaga accgggcatc gctgacgatc
      901 gggcgccccg agtacaggga tctcttcgcc gcgctagagc ggtttcgaga ggctgcaggg
      961 aagcgagtta tctacctgcc agggaaccac gatgcggagt cgttctggaa caaggagatc
     1021 caggagacgt tgcgcgagcg tggcttggtc gacgaattcg cttactacta cctggcctcc
     1081 gtggagacgg gcggggggcg gcgggtagtc tactgcgagc acggcaacca gttcgacccc
     1141 gaaaaccgcg tcggggatta ccacgatcct ctggacacgc cggttgggca ccacgtcgtg
     1201 atggacggca ccaggcgcat cgcgccgttc ggtgagatct cgtcagggtt ggacctttca
     1261 gagataaaga tggtctaccc gctggtggcg attccggcct ggatagctag ccgatacttc
     1321 tacaacatgg caggcaaggt cgcttcttac ctgctcctgc cgctgctcgt ggcgtacatg
     1381 gtctacaagg tggtcgcgtt cgctatcgcc cgcgcgaccg acggtaaggc gagcctgctg
     1441 ttcggcagtt accgcgagtt gccccacgtg caccaagctt ttctcgacac cgtcctcttt
     1501 ctgctcgtgg tgtttgggat cttcggtgtc ttcttcctgg tcgtgcgcca cgccgttcat
     1561 aggacgttga gggcggtgag cccgggcgga acgccgcatt actcgcccgc ggaggcctcc
     1621 cagaagagga tcagggcggt cctcgctggc gaggcgaggc ccccgatgga ccccgcgttc
     1681 gatccaacga ccgccgacgt ctttgtctcg ggacacaccc acctcccttc gctcgccgag
     1741 atggagcggc ctgatggtaa caaggcggtg ctggtgaact ccggatgctt tttgcggcaa
     1801 ctgcagccca taacgcccag gctaaagggg ccgccagtct tcgtttcgaa gttcgtactg
     1861 acccacgtca gggtgtttgc gcagggagag aagttgcgtg tcgagctgtg gcagcagccg
     1921 aagccggccg gacagaccct gacacgcatc gagcgtctcg tctcgatcgg ccgccggccg
     1981 ccgcagccgc ccgcgggggc caagccccgc ttagtggcct cggctaccgt gtagttggcc
     2041 gtgcagcgtg tctcggacgg agtgttgggg tatacataat ctgcactact tttgagttga
     2101 ttggaagaga tccggaggga ccagttggct gatcaagagt tagaccagca gttcgtagag
     2161 agccagcgag agaggctggt aggtttgcgc gatgaactgg tgcgcataag agaaggcatg
     2221 gccgatgacg aaagggacct cggcgaggcc gagggggaca cccagctcga ctctggtgac
     2281 ctgagtcagg atatgttcac ccgggagatg gacgcctcca taggggaaca ggtggggcgc
     2341 cgcctcgggg aggtcgaccg ggcgctgcag aagatagaag agggcaccta cggcctctcc
     2401 gacgacagcg gggaatccat acccaggggt aggctcgagg ccgtgccaga agcgctaagg
     2461 accgtcgagg agcaacagcg cttcgagcgg gagcgacgac ccccgatcta gggtcacctg
     2521 gcggtataat ctgtctatgg atcatcgttc gttaggcgac gacacaagag agcccatctt
     2581 ccgcagcgac ctcgaagagc cgaggccaga acccacgtat gagcccctga gaccagaagg
     2641 cggcctgtgg agcgtcatca aacgctgggt tgcaccgctc gccgcggtag ggctcttgct
     2701 agcgaagttc aagggcctgg cgcttctcct tctcaaggtc aagtttctgg gcactgcgct
     2761 gaccatgctc gttagcatag gagcttatgc gctgttgttc cccgtctggt tcgccgtcgg
     2821 gatagtcgtc ctgatctggg tgcacgagat ggggcacgtc ttgcagctga ggcgcgaagg
     2881 catcccggct tcagccccaa tgttcatacc ttttctcggg gcattcgtcg cgatgaagca
     2941 gatgcccaaa gacgccctgg ccgaggcacg cgtcggcctc gccggccccg tgctcggtac
     3001 gctaggaggg ctcgcgacgc tcgggctcta cgccgccact cgggagccgt tgttcctggg
     3061 gctcgcatac ttcaacttca tcatcaacct gttcaacctc gcgccgcttc tcccgctcga
     3121 cggcggcagg gctgtcgggg ccatgtctcc ggtcttccag ctgctcgggc tcgtggtgat
     3181 ggtcgcgctg ttcttcgtgg ccccgatcat ggcttttatc gcggtactcg gcttgcctga
     3241 gctctggcac cgctggaaga cgcgtaatac gctggaaggc cgggcgtact acgacctccc
     3301 actgcgccag cgcgtcgcgg ttggtctggt ctacctgggt ttgatcgcgg tactcggact
     3361 tctgacgggg gctacgttcg tgccggatcc ggcggcgctc cagtagcttg cgtacgattc
     3421 tctacacggg gaagggcggc gtcggcaaga cgagcgtggc tgcggcaacg gccctgaagg
     3481 cttcgcttgc gggaaagaaa gtgctcgtga tgagcaccga ccctgcgcac tcgctcgccg
     3541 acgtcttcga cacggagata gggcaagacc cgaaggagat ggcccctggc ctgtgggcgc
     3601 aggagatgga ccacacctcc atgatcgaag acagctgggc cgagatacag ggctacatga
     3661 ccacgttgtt cgagtggcag ggcgccgaca cgctcgccgc cgaagaactg gcgatgctgc
     3721 ctgggatgga cgagcttttc gggctcctca tggtcaggcg ccacaaccag gaggaccgct
     3781 acgacgccct gatcctcgac gctgcgccta ccggggagac gctgaagctc ctgagccttc
     3841 ccgaccacat gagttggtac gtggagaaga tcttcccgat acagcgccgc gccgcgaagc
     3901 tggtcaggcc ctttgcgaac aggaccaggt ccctgccccc gctaccccag gacagcgtct
     3961 tcgctgcggg acagaggttc tacgaggcca tcgcgagcgt cgaggagatc ctgaccgacc
     4021 gcgaaagcgc ctctgtgcgc ctggtgctca acgccgagaa gatggttatt gcggaggccc
     4081 gccgcgccta cacgtacctg aacctctacg actacggcgt ggacgccctc gtggtcaaca
     4141 gactcctgcc cgagaccgtg agcgaccctt acttcgcgcg ctggcacgat gtgcaggcgc
     4201 ggcacatgag gacgatcgaa gaggctttcg accccatccc aatcctgacc gcgcggctct
     4261 tcgaccgcga gatgtttggc ctggaggcgc tcggcgcgct cgccgaggac gtcttcgagg
     4321 acgcggagcc tctgggggta ctgttcaagg gcgccacgca cgacatagag aagaatgggg
     4381 acggctatga cgtcgtcttc cacctaccgc tcgccgagaa gcggaacgtg gacctgtcga
     4441 agaagggagc cgagttgctc gtaaaggtcg gcaattaccg gagaaacatc cttctgcccg
     4501 acgccctcgc gcgcctccct gcggcgggag cgagcttcga gggagggtcg ctgaaagtga
     4561 gattgaggga tggctgaaga gaagcgacac agaggcaagc tgctcggcgt gatcgcctac
     4621 gtcctcgtgc ccggcgaacg gcgcgtgcgg gccgccgcac actttcgcaa agcgggcttc
     4681 gaggcgctga aaggcgtagg ggagctggtg cgacccgaga agtcgcagac gtccacagga
     4741 ccgacgagca gggaacgcat cgagatcgag tagggaagtc cgttgacggg ttccaggaga
     4801 gggtgatgag aggacaggag ttgctgcaga gggtagggga gtttgccagg aaggaggtga
     4861 ccctggccct gcctgcggcg cagcgggccg cgagccacgg catacaggcg ttcctcaaga
     4921 agttctccga agagtatgcg atcgagcgaa ataaattgaa gagtggtgcg agtacgccgt
     4981 ccaggtttgg ccatcaaggg cgccggtagt aagatatccc ttacaaattc tagtagatac
     5041 cagcgggaga ttatatggtc gagaacggac aattaacggg gaccttcagg gtcaagagcg
     5101 ggatggccca gatgctcaag ggcggggtca tcatggacgt cgtcgacgcc gagcaggcca
     5161 cgatcgccga ggaggcgggg gctgtggccg tcatggcgct ggagcgggtg cccgctgaca
     5221 taagggccga cggcggggtt gcgcgcatga gcgaccccga gaagatcgtc gagatccagg
     5281 agtccgtcac cattcccgta atggcgaagg cacgtatagg gcacttcgtg gaggcccaga
     5341 tcctggaggc cctcgaagtg gactacatag acgaatccga ggtcctgacc ccagccgacg
     5401 agaagcacca catagacaag gccgccttcg aagtgccttt cgtttgcggt gccaccgacc
     5461 tcggagaggc gctgcggcgc atcggcgagg gggcggccat gatccggtcc aagggcgagg
     5521 ccggcacagg caacgtggtc gaggccgtgc ggcacatgcg cgccatagtc ggcggcatcc
     5581 gccgcctggg tgcgctggag cacgaggaga tgatgacgga ggcgaagaac ctcggggcgc
     5641 cgtacgagct cgtgcggtgg gtcgccgaga atgggcggct tcctgtcgtg ctgttcacgg
     5701 ccgggggcat agccacccct gcagacgcgg ccctgatgat gcagctcggt gccgacggcg
     5761 tgttcgtggg tagtggcatt ttcaagagcg cggaccccgc cctgagggct cgcgccatcg
     5821 tgaaggccac gacccacttc aaggacgcga aggccgtggc cgaagcgagc aggggtctcg
     5881 gcgtggcgat ggtcggcagg gagatgggcg acctggctga aggagagcgg ctcgcgaccc
     5941 gtggctggta acggcgagac gaacggcccg aagaagatcg gggtgctcgc gctgcaggga
     6001 gacttcaggg agcacgccga gatcctgcgt gggcttgggg tcgagcctgt cgaggtgcgg
     6061 gccgtggagg acctcgaagg actggccggg atcatcgtcc cgggcggcga gtcgaccacc
     6121 atagggaaga tgatggcctc ctcgggcctc ctcgacggta taaggagctt cttctacaag
     6181 ggcggtcctg tgtggggcac gtgcgcaggt atggtcctcg ctgcgtcggc gacgacgggc
     6241 ccgcgccagc cgctgctcgg cctgatgaac gcgctcgtcg agcgcaacgg gttcgggcgg
     6301 caggtccatt ctttcgagaa ggacctcgaa gtagagggct tcgacgagcc gttcaccgga
     6361 atatttatcc gggcgccgtt cttcgaggac gtggggccag gggtcgaggt gctctccagg
     6421 atcggagatc gggtcgtggc ggccaggggc gagaacatac tggtaacggc gttccatccc
     6481 gagcttacgg acgacgccag gtttcacgag tactttttgt gggaggtttg ttccatatga
     6541 gcggtcacag caaatggtcg acgatcaagc gcaagaaagg cgccgctgac gccaagcgcg
     6601 gggcgctctt cggcaagttg tccagggcga tcagcgtcgc ggcgcgcgag ggtggaggtg
     6661 acccggagat gaatgcccct ctaggccttg ccatacagaa ggccaaggac gccaacatgc
     6721 ccaacgacaa catccagcgg gctatcgaca agggtacagg cgcgggtgcc gacgacgata
     6781 cctacgagcg gatcacctac gagggctacg cgccgggtgg cgttgccgtc ctggtcgacg
     6841 tgcttacgga caatcgcaac agggccgcct ccgacgtgcg ttacgtcttc tccaagaacg
     6901 gcggcaagct gggcacgagc ggctcggttg cgtacctgtt cgaacgcaag ggcgtcatcc
     6961 tggtccccag ggactccaca gacgaggaca cgttgatgga ggtggccctc gaagccgggg
     7021 ccgaagacgt agaagaacag gagaacgact acagggtcgt gacgaccccg gaagatttca
     7081 cggccgtcag agaggccctc ggtgaggcgg atatccctta cgagaacgcg gagatcacca
     7141 tggagcccca gaacagcatc gacctcgacg ctgggacggc gaggcagacg ctgcgcctga
     7201 tcgacgccct cgaagagaac gacgacgtgc aggaagtcta cgcgaacttc gacatctccg
     7261 aggaggtcat ggcggaggtg gtttcgtagg gcgtcctgct ttcgccggca ccttctctgg
     7321 ccgggtcgga ctaggaccct ccatcaagaa gatcctcgtg gccttgctcg tggcgctgct
     7381 agtgagttcg gcctgctctt ccggtgacga ggggaagggt ggagatgagg atgtgatcgc
     7441 cggcgcctcc aaacagcgca ccatggatct cccaccgaaa caaacggaaa ccgatggagg
     7501 gataggcgac ggcgtagaga ctggaaacct gcgtttccgg atcttcgagg tgcgggccaa
     7561 agaccgcgtc tacgccatgt cagggcctgg gaagagaccc gtaacgcggg ggaacatctc
     7621 cagcgaatac gtcgctatag attatctggc aaagaacctc tcaggttcac cacttaccac
     7681 gggggcgaag gcaacactcc tcgatgacca gggcaattcc tacgggcagg acaagtctat
     7741 cgagcctcct tccggcggca ccgacggtat ggaactcgga acggcccaga caaaggcttc
     7801 cacgatgttc ttcgaggtgc ccaacggcgt catccccgcg acgctcgaga tagaggcttc
     7861 gggaggcgag gccagcttcg atctgctgca gaggaatatg gagagagtgc cgcccgacga
     7921 ctacctgcgg gtgtaccacc tatacttcaa tgaacaggcc tacgaggagg cctacgagat
     7981 gttcgacccg gcctccgtcc agaacatcac gctcggcgaa tggctgactt tctacgagcc
     8041 gctgtggggc aaacgatacg tgagcctcga tggcctaaca ccactttcgg aagactctga
     8101 tagtgcgact tttcagatga ggagaacctt ctacgacgcg gacggcgaca tcgttgcgga
     8161 cccggagatc aacccttccg taatacagga gatggtcgag gtagatggag agtggaagct
     8221 cgtgatgcgt gacgacctgg tctcggacat catagctgtt ataggacctg acgagacacc
     8281 caggccgaag gcgcccgaac ctgagaagaa aggcccagaa cgcacccagc cgccttcgac
     8341 tgttcccgag accactgagc cgtccgaggc caccagatcc gcggccccgt cacgcgacta
     8401 cgactgcgat gatttcaaga accaggaaga ggcccagctc tacctcgcgc caggagaccc
     8461 ctacggactc gatgaggacg gcaacggcgt ggcttgtgag accttgccgt agaggacgtg
     8521 agagtccccg cgcatggact cacattcgtc agatgtgctc gtttgtcgct gaagggagag
     8581 tcagcgctta cggtttggag atgtcgaccc ccgaaccacc aggtgggttg ccagcggttc
     8641 cgcgctcggg gcgtcttcat cgtgaagctg tgctaccagc atgcgtccgg ccattgtccc
     8701 cttctcagcg tggtcctggt ggatggcggt gaggggagga tcggcgctcg ccgccgcggg
     8761 gatgtcgtcg aagccgacaa cggaaacgtc ttggggcacg gagatccgcc actcggtcat
     8821 ccattcgata gcgccaagcg cgagctggtc gctcaaggcg aggatggctg tcggctcctg
     8881 ctccgaacgg aacaacgcct cgatggccca gtatccgagt tctttggcgc tgccctgaca
     8941 ctcgtagacg ggcacctcag accagggcag acctgcttcc tccatagcag cccggtagcc
     9001 ccttagcctc attcggctaa cgtggaatgc ggtcccctcc tggcggtctg agtctgcgat
     9061 cccttcgcgg ccgtccggtg cgaggccgaa cgacacgatg gcgaacctct cgtgcccgag
     9121 gtcgagtaga tgctcggccg ccattcgggc cgctccctcg tcgtcgacgc ccacgaacgg
     9181 cactccctcc tttcgtggct ggtccaccac tacaaccggt aatcgcctct gtaaagctgc
     9241 cgccaacaaa ggttcctcat cgctcatcga gtagataacg aacccgtcca ccgcggcctg
     9301 cgctgccgcc cgagggtcgc gctctgaaga ctctccgccc ccggccgccg gcacgagcag
     9361 aagtcccaga tcggcctcct ccgtgaccga actcagaccg cccaggaaag agaccgcggc
     9421 aggatcgtcg aaggcgtagg aaagcggatt cgagtacagc accccaacct cgtccgtcct
     9481 ctggcgtctc aacgaacgcc ccacgggatc cggaccgtgg aagccgagcc tcccggtcgt
     9541 ctcgaagatc cgctccctca aggcctcgga gagccgatcc gggtggttgt aggcgttgga
     9601 gaccgtcatc gccgatacac cgagcttcct ggcgatactg gtgagggtca cgcggttccc
     9661 gctagaactc atgcctttgc cttcttgcgg cccacgaccc acatggtaca ctactttaac
     9721 gttaaaagta tatcgttaaa gtctatgtgt ggaggagcgt cgtggatgta ggaggccaca
     9781 ctcgggtcga gcgcaaggat gcggcgctga ttctgttgtg tgtggcgcag ttcgtggacg
     9841 tgttggacgt aaacgcggta atagtggctc tgcattcgat cgggcaggat ctcgggttct
     9901 cgcaggcggg tctgcagtgg gtggtctcgg cttacgtgtt gttccttgcg ggcttcttgc
     9961 tcttggcggg gagggtggcg gacctgtggg gtaggaggag gaccttcgtc gtggggcttg
    10021 ccgtcttcac ggcggcttcc ctttgttgcg gcctctcggt ctccaaggag atgatggtgg
    10081 tctcgcgcgc cttccagggg cttggagcgg cgaccaccgc cccggctgcg ctctcgatca
    10141 tcaccaccat ttttgccgag ggccgagagc gagaccgcgc tgtggccgcc tggacggcgg
    10201 tggccgcggg gggtggggct gcgggattgc tccttggcgg cttcatcacc gacgttttgg
    10261 gctggcagtg gatcttcttc gtcaacgtcc ccgtgggcgc ggctggtatc gcgctctccc
    10321 aggtgcttct acccgagtgc cgggaccctg cggcctcccg caggttggat ctcctcgggg
    10381 ccggaacggt cacatcgggg ctggtgctct tggtcctggc gctcacgagg accgaagagg
    10441 cgggcttcgg ctcaccaatt acgcttgcga cgctcggctt cgcagccgcg ttgttcgggg
    10501 ccttcgtgtt cgtggagcgg cgcgccgctg atcccctggt ccccttgaga ctcttccgcc
    10561 tgcgcgggtt ggtgggggcg gccctggtcg ctttcgcgat caccgccgct accgccccgg
    10621 tcggcgtgct ggtcaccctc tacctgcaga acaccctggg ttatcctgcg tcctatgcgg
    10681 ggctggcggg cctgccgttc agcctctgcg tgatcgccgg ttcgatgctg ggcggtagga
    10741 tctccgggcg aatcggcggc agagtgacga tgtccctggg gcttgctgtg gtagcggtgt
    10801 ccgccctcct taccgcggga atcagcgccg agggtggggt ggcctacgtg ctcgcgggcg
    10861 ccgccctctc cgggttgggg ttgggctgcg cttcggtcgc ttcgaccgct cgcggtacct
    10921 cggcggtgga ggaagggaag cgcggtctgg cctcgggttt catgaacacc tccgcgcaag
    10981 tgggaacagc gctgggattg gcggccctcc tcaccctcgc ggctacccgc actgccgcgc
    11041 tatacgatgg tgcagaatcc gcggccgagg cgctggtagc aggctacagg ctagcgttcc
    11101 tggtcgccgc gggcgtcgca tcactcggcg tggtggcggc tctgtacttc ttccgccagg
    11161 ccaaagattt tgatacctga agcagtggtg tgcggaaggt tcgaatctca ttgcaaggga
    11221 cgcttgtagt gtcggccatc caatgtacaa gatgtagaag acggtgcaag cggaggccgg
    11281 agggagtaat cttaggccat gagccccacc ggcggcatcg gacacaccat actcggcata
    11341 gacccaggga cggcgacgat ggggtggggt gtgatccggc gggaaggcaa ccggttgcgc
    11401 tacatacagc acggcgctat catcacgccc cccgactggg agatgccccg caggctcggg
    11461 cggctcttcg acggagtaac cgaactggtg cgaggctaca ggcccgaaac catcgcggtc
    11521 gaggagctct ttttcaacac caacgtgact acagcgatca cggtcgggca ggcgaggggc
    11581 gtagcgctgc ttgcggcgta caaggccggg atcgaagtga ccgagtacac gcctttgcag
    11641 gtcaaacagg cgatcacatc gtacggtagg gcggacaagc gccagatgca agagatggtg
    11701 aaagccctcc tcaacctgcc cgccatacct cgtcccgacg acgcggccga cggactggcc
    11761 atcgccattt gccacgcgtt ctcaagcact atgcctggca aggtaggggt ggggaaatag
    11821 aggatcgtgc gtgataatta gcccatgatc tacaggttgc gcggtactct ggtcgagaaa
    11881 gatacggaag gtgttgtaat cgatgttggg ggcgtcggct acaggacctc ggcttcactg
    11941 gcgacgttgc gtgcgttacc ctcgctgggc gaggagtgcg ttatccacac caggatggtg
    12001 gtgcgcgatg acgcgatact tcttttcggg tttgcggtgc gcgaggagcg ggccgcgttc
    12061 gacgcgctga ccgcggtgag caaagtcggg cccaagctgg ccctctcgat actctcttcg
    12121 atgtcgcctc ctgagatctc cgagacggtg gccagaggag atgtgatcaa gttcgcctcc
    12181 gtgccagggc tcgggaagaa gacggcggaa cggctcgtgc tcgagctcaa gggcaagagc
    12241 ctcgccgcct tcggccctga gccggtcgtc gcaggcggcg gtgggggtgg cccgtacatg
    12301 gaggcaaggg acgcgctaac ggcgctcggg tacggactcg aggaggccga gaaggccctc
    12361 aacgacgtgc caccgcagga ttccgtggag aagtatataa aggaggcatt acggc
//
LOCUS       DASTKM010000114         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_731542, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000114 DASTKM010000000
VERSION     DASTKM010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731542"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..567
                     /locus_tag="VFQ10_04945"
     CDS             226..567
                     /locus_tag="VFQ10_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9964719.1"
                     /translation="MAEGPVLVERPGEGIAKLTLNNPPLNLVTLEMTEQLIEALEELE
                     GDEAVRAVVVTGAGDRAFCAGSDVKEFAEVRDRVVQMKLARENEAFGRFESLSKPTVA
                     AIEGLAYGGGV"
     gene            575..1036
                     /locus_tag="VFQ10_04950"
     CDS             575..1036
                     /locus_tag="VFQ10_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9964720.1"
                     /translation="MACDLRITGEGARFALPEVRLGVVPGSGGLFRLPELVGPARAME
                     LMYLGESIDASEAERLGLVNEVVPDGEALPRALDVARCISRQPKEAVVAIKRGVRESL
                     HSSREDSVRLTLELSDHVFRTEDCAEGILAFFEKRETRFAGAPGTGDEVEV"
     gene            1090..2271
                     /locus_tag="VFQ10_04955"
     CDS             1090..2271
                     /locus_tag="VFQ10_04955"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9964721.1"
                     /translation="MLDGVKVLSFTHFLQGPSAVQILADLGADVVKIEPPKGAWERHW
                     SGAEAYLNGVSVFFLLSGRNQRSLGVDLRSERGKEIVWRLIRDSDVIVENYRPGAMER
                     LGFGYEKVSEEKPDLVYCSLSGYGTDGPYRDRAGQDLLSQAMSGLATINGRKSDPPTL
                     VGSAVVDQHAAVLGALGVLAALYERRDTGKGKRVDSNLLNSALDLQIEPFSYHLNGQL
                     YDRSETGVSSRFHQAPYGIFETADGYICISLTPPEKMAEAFKDDTFRGWTEKDQFRRR
                     EEVNVHIAGHVRTRPAEHWYGVFDEVGIWYAPVNDYEDVEGDPQVVHNQAIMTFEHPK
                     AGKVRVLSHPVRYDGEAPPLRMLPPELGEHTEEVLSELGYGQVEIEDLKSEGVVRAEK
                     H"
     gene            2255..>2632
                     /locus_tag="VFQ10_04960"
     CDS             2255..>2632
                     /locus_tag="VFQ10_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HET9964722.1"
                     /translation="MQRSTDAEKPVDRAIPLEVGDAVTFSKTVGESDVYLFAGITGDF
                     SPNHVNKEVMGGTPYKERIAHGVLSIGFASTTSTLMIEKSGAKAVSLGYDRIRFIGPI
                     FIGDTVTVTYTISEIDEENLRTRA"
BASE COUNT          511 a          792 c          873 g          456 t
ORIGIN      
        1 cgacgcggct aggtcaagag ccgcgggcgg cctcttctct tccgccacct gaattacggt
       61 tcgcacctcc ccttatatcc taaccgcttg accacgtccc atactttccc tatgcttccc
      121 gtggagtgag taaagattct gttgtgtgaa aactaactgt ttcgactcac ggtcttcctc
      181 agcctctcgc tgtggctttc cgggcttatg ggggtgtact agaccttggc cgaaggacct
      241 gtgctcgtgg agcggcccgg cgaaggcatc gcgaagctta ccctcaacaa cccgccgctc
      301 aacctggtca cgctggagat gacggagcag ctgattgagg cccttgaaga actcgaagga
      361 gacgaagccg tccgcgcggt cgtcgtcacc ggcgcgggcg atagagcgtt ctgcgccggc
      421 tccgatgtca aggagttcgc tgaagtccgc gacagagtcg tgcagatgaa gctggcccgg
      481 gagaacgagg ccttcgggag attcgagtcc ctatccaagc ccaccgtcgc ggccatcgag
      541 ggcctggcct acggtggggg ggtgtgagat ctcgatggcc tgcgatctca ggatcacggg
      601 cgaaggcgcg aggtttgcgc ttcccgaagt aaggctcggc gtagtgcccg ggagcggcgg
      661 tctgttccgc ctccctgagc tcgtcggccc ggcccgggct atggagctca tgtatctcgg
      721 cgaatccatc gacgccagcg aggccgagcg tttgggactg gtcaacgagg ttgtccccga
      781 cggggaggcg ctcccgcgtg ccctcgacgt cgcaaggtgc atctcccgcc agccgaaaga
      841 ggccgtggtg gcgatcaaac gcggcgtgag ggagtcgctg cactcgagcc gcgaggactc
      901 cgtcaggctg acgctggagc tgagcgacca cgtcttcaga acggaggact gcgccgaggg
      961 gatcctggcc ttcttcgaga aacgcgagac gcgttttgcc ggagctcccg ggacaggcga
     1021 tgaggtcgag gtatagggaa tttgttcgcc gcaggttgcc ggctactcat ggagaggatc
     1081 ggatagacga tgttagacgg ggtaaaggtc ctgagcttca cgcacttcct gcaaggcccc
     1141 tccgccgtcc agatcctggc cgacctcggg gccgatgtcg taaagatcga accccccaaa
     1201 ggagcctggg agcgccactg gtcgggggct gaggcgtatc tgaacggggt tagcgtcttc
     1261 ttcctcctca gcgggcgcaa ccagcgaagc ctgggcgtag acctgcgctc cgagagggga
     1321 aaggagatcg tgtggcgtct gatccgcgat tccgacgtca tcgtcgagaa ctacaggcca
     1381 ggcgctatgg agcgcctggg cttcggctac gagaaggtct ccgaggagaa gcccgacctg
     1441 gtgtactgct ccctctcggg gtacggcacg gacggcccgt acagggaccg ggctggccag
     1501 gacctcctga gccaggccat gagcggcctg gcgacgatca acggccgcaa gagcgatccg
     1561 ccgaccctgg tcgggtccgc ggtcgtcgac cagcatgcgg cggtgctcgg ggctctgggc
     1621 gtccttgccg ccctctacga acggagggac acgggtaagg ggaaacgagt cgacagcaac
     1681 ctgctgaact cggcgctgga cctgcagatc gaaccgttca gctaccacct caacggacag
     1741 ctgtacgacc gcagcgagac gggcgtctca tcgcggttcc accaggcgcc ctacggcatc
     1801 ttcgagacgg ccgacggtta catctgcatc tcgcttacgc cgccggagaa gatggctgag
     1861 gcgttcaagg acgacacctt ccgcgggtgg accgagaagg accagttccg caggagggaa
     1921 gaggtgaacg tccacatagc ggggcacgtc aggacgagac cggcggagca ctggtacggg
     1981 gtgttcgacg aggttggcat ctggtatgcg ccggtcaacg actacgagga cgtcgagggg
     2041 gacccgcagg tcgtccacaa ccaggcgata atgaccttcg agcacccgaa ggcgggcaag
     2101 gtgcgggtgc tctcccaccc tgtccgctac gacggggagg cgccaccctt gcgcatgctg
     2161 ccccccgaac tcggagagca cactgaggag gtactctccg agctgggcta cgggcaagtg
     2221 gagatcgaag atctgaaatc ggaaggagta gtgcgtgcag agaagcactg acgcagaaaa
     2281 gccggtggac agagccatac ccctggaggt cggcgacgcg gttacgtttt ccaagaccgt
     2341 cggcgagtcc gacgtttacc tgttcgccgg catcacgggc gacttcagcc ctaaccacgt
     2401 caacaaagag gtcatgggag ggacgccata caaggagcgc atcgcccacg gcgtgctctc
     2461 cataggcttc gcctccacga cctcgacgct catgatcgag aagtcggggg ccaaggcggt
     2521 ctctttaggc tacgaccgca tcaggttcat agggccgatc tttatcggtg atacggtcac
     2581 ggttacctac actatctccg agatcgacga ggagaacctg cggacgcgtg ca
//
LOCUS       DASTKM010000115        10767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_378755, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000115 DASTKM010000000
VERSION     DASTKM010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378755"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFQ10_04965"
     CDS             complement(<1..213)
                     /locus_tag="VFQ10_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9964723.1"
                     /translation="MAEGPVLVERPGEGIAKLTLNNPPLNLVTLEMTEQLIEALEELE
                     GDEAVRAVVVTGAGDRAFCAGSDVKEF"
     gene            complement(422..1396)
                     /locus_tag="VFQ10_04970"
     CDS             complement(422..1396)
                     /locus_tag="VFQ10_04970"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET9964724.1"
                     /translation="MRPELVLRGVIPANLMAFDSELEIDEENYRRHLRYLIDTPGVAG
                     LTTNAHASEVATLTFEEQQRVLEMTLDEVDGTIPVVCGVYEDGTSKAARIAKSAERQG
                     ADCLLVFPSTVYDFGHQLRPEMAYGHIAQIAEAVSLPIVVFVYPVTSGLHIPTDSLVR
                     ICDEIDNVIAVKEWSNDIGVYERNWRELGALDKEISLLSSFSKSLLASLCIGADGILS
                     GHGSVIADLHVEMFEAVNRGDLKGARRVAGRAYPVVQATYAEPFLDGHNRMKEALAIL
                     GRIDEAHVRPPLRRIPDAEREKIRRVVAEAGLPGASREQPDAALGRRG"
     gene            complement(1579..2004)
                     /locus_tag="VFQ10_04975"
     CDS             complement(1579..2004)
                     /locus_tag="VFQ10_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET9964725.1"
                     /translation="MRQLAAVLFSCALFSVVLLTGAPSEAHAQGYSGQAVVDDAQTYI
                     GTPYGAWGMDCSGFTSAVFADLGVYLPDSPDAQYAYGTPSYGEAGDLVFFDEAGYGIS
                     HVGIATGYGTVIHASTYYGAVVETPIENIPGYVGTVDAY"
     gene            2532..2783
                     /locus_tag="VFQ10_04980"
     CDS             2532..2783
                     /locus_tag="VFQ10_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964726.1"
                     /translation="MYEMMDPQVSRQRREEMLHQAERGRVAGALPADCKRRPDRTLAW
                     ELQRVAGLLLKLLRRSKKMLMARKSTPDGMKRARRKKYG"
     gene            2776..3510
                     /locus_tag="VFQ10_04985"
     CDS             2776..3510
                     /locus_tag="VFQ10_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964727.1"
                     /translation="MAEREGMVGGEGLGSGAISNVGVLDLTGMKSTEELAGIGSISRV
                     GIVLVPETLMSGLLAVPMEKVGATVPVPAGENVKLVTGQMKTTGEALASAGREEDVLV
                     VAGQLLVTTPVERVGYGEMIVAGQVYAPQGSEGALTAGISRLIGQVFYYSGKPRFFVG
                     SERFGKGFFEVLEGPMAMILVGDFAIERGVPADLLKEKISQILLAGNLQAPQELLPLV
                     QVLTVEKAGNISALEEGDEDGERTEP"
     gene            3540..4112
                     /locus_tag="VFQ10_04990"
     CDS             3540..4112
                     /locus_tag="VFQ10_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9964728.1"
                     /translation="MVDRTPDGATSGLVRCFNRIYDANHRALHAYFLGRTSDPEAALD
                     LSQEAFLRVWRNVSKLQELTPQRQRYWLYTVAKNLVADLYRGSASQRAARQSVVARLR
                     SGGNHEEGPQAALESRERLEAVDRAMNELPEDLRTALLLQVMGRLSSSQIGEALEVPA
                     GTVRYRIAMARKRLAELLRLDEHLGTKGRT"
     gene            4109..4438
                     /locus_tag="VFQ10_04995"
     CDS             4109..4438
                     /locus_tag="VFQ10_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964729.1"
                     /translation="MTDHNLDLPSDTDARLDRLLEEWVEQRGLTKARTEQLRQAVLAM
                     HELDAATPALAAASLDQGAEWWSSLFTPLLTALSQAATTGYLSVGTGMEAEPDPSVGY
                     RAYMRLA"
     gene            complement(4475..5830)
                     /locus_tag="VFQ10_05000"
     CDS             complement(4475..5830)
                     /locus_tag="VFQ10_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HET9964730.1"
                     /translation="MRSVSRLNILIAITAAIIDLIYGYDLGSIASALLFLVPAFDLTT
                     FMTSVVTSAVVLGQLFGALFAGRISNTIGRKRSLVLVALGYAAFAGLQGLAPNEWFLT
                     VVRFLLGLAIGISIVVAPAYIAESAPVSVRGSMLVTFQIATTSGIAVAYFVGVALAPT
                     ESWSLILSLSAIPAVIVLILIVRLPDTARWLLMNGRREEAADLLRRVEPDMDPDEEVQ
                     RIEHDLSYEEKGSFAELFQGRFRKAGIFVVGLGFLVQITGINAIVYYSPTIIQEVGVT
                     SPTGAIIATGFVQVAGVIAEVIAFLVVDRWGRRPTLLTGIGTMAAANAILVLAYALNS
                     PATFTLLGILLFTVGFNFGYGSLVWVYASESFPVRLRTQGGSAMLTADLFANFIVGVV
                     FLGALGALGGSVTFGIFFALALVSFAFVYVLAPETKGRPLEAIRSYWYNGGRWPEETS
                     T"
     gene            5964..6983
                     /locus_tag="VFQ10_05005"
     CDS             5964..6983
                     /locus_tag="VFQ10_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET9964731.1"
                     /translation="MKRLHVYSDPATERHVNDLAGVESPKRFPAALAGVERAEQSGVL
                     VERRPCSPAERDALTAVHDPAYLDDLQEMSAAGGGYLSLDTAVGAGSWEAALLASGAA
                     CSAVESVLAGETAFAVARPPGHHAGRDYGMGFCLTNHAAVAAGHALSRGLERVAILDW
                     DVHHGNGTQDIFYGDGRVLYLSVHQSPFYPGSGEASEVGKGEGLGFTVNLPLPAGSEE
                     DLYAAAFSGVFLPVLREFGPQVILISAGFDAHVDDLLGGMALGSDSFGRFAALISDLS
                     REIEAAPPAFVLEGGYNLDALTESVAATMQGVGERAPEWEYAGGSRHIQKAREALAPF
                     WRSLR"
     gene            6988..7665
                     /locus_tag="VFQ10_05010"
     CDS             6988..7665
                     /locus_tag="VFQ10_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HET9964732.1"
                     /translation="MRIFGFPPFEDRRDAGRRLAGKLSRFKDERTVVFALPRGGVPVG
                     YEISRALEAPLDVFVSRKLGAPGQPEFGIGAVAAGGVRVLNQDVVRRLGIPDEYVQEI
                     TAQELAEVGRRLRYFRGQRPETEVVGRTAILVDDGLATGVTARAAVQALRLRKPRRLV
                     LAAPVCAAQTADLFLRMVDDLVCLESPSDLGAIGFWYRNFEQTSDEEVVELLERARSE
                     RGEKGSP"
     gene            7759..8421
                     /locus_tag="VFQ10_05015"
     CDS             7759..8421
                     /locus_tag="VFQ10_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET9964733.1"
                     /translation="MCGRYTLSTPAGRLAEEFQLDEVSVDLAPSYNVAPTQEVAAVLE
                     EEGKRRLEMLRWGLIPSWADDPEIGARMINARSETAAEKPSFRSAFRRRRCLIAADGF
                     YEWKRENGGKQPYYFHMQEGRPIAFAGLWESWDKGGGALRSCTILTTRANSVLEGIHD
                     RMPVILPSGAYDLWLDPDADREQLADLLIPYPGDDLETYPVSRFVNSPRNNDERCIEP
                     AA"
     gene            complement(8615..10000)
                     /locus_tag="VFQ10_05020"
     CDS             complement(8615..10000)
                     /locus_tag="VFQ10_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET9964734.1"
                     /translation="MRRVSRFNLTHCGGLRGLAVLLGIVGAMCALSIAGNSVSSAEAL
                     RESKGPVERTEGEVVGASIPHPVKWFVERERYSYGQTYGFTLWRPDSGAPHDHGGTPA
                     VRVALAYGLRPGQIRATVLEKLRAYPNLPMTREQVSVAEKGHRGVAVGPIPGSTPSTE
                     VYVPVNGRVYQINVYGKTSGSDAKELLSTLRFEPPSRPVSSLGLPVANSVKTLYGAGD
                     PELAERERTIHEATTEGEATFAASSLGEETPIEEGCWQADPDFFFQTQYGSTANANPE
                     DSVPTGWTIIGRPNYWDEYTHGRLEYGRCDRRHYTNDKFAVDYLLDPGDAIFSPFRRG
                     TVTFAGRNITHKDYGIFVSIKAANGKYVNVSGHLSRVAPGIRRGAVVDRNTVIGFAGR
                     TGGGDIPVGPVHLHQAYYRYPTYTSDGAPYGGAGLKVVDHHYYRGDNGVYRFGWVERQ
                     GFKYKGSWISF"
     gene            complement(10171..>10767)
                     /locus_tag="VFQ10_05025"
     CDS             complement(10171..>10767)
                     /locus_tag="VFQ10_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9964735.1"
                     /translation="DEYPDVVIGCVGGGSNFGGVAFPFVRENLKNGKSTRIMAVEPAS
                     CPTLTKGRYTYDFGDTAGTTPMMKMYTLGHSFVPSGIHAGGLRYHGDSPIVSALAHEG
                     LIEARAYAQNPTFEAGVTFARTEGIVPAPEVTHAIKATIDLALEAKEAGEQQTILFNL
                     CGHGHFDFSAYQQYLAGELVNLEHSDEETEAAVAGIPG"
BASE COUNT         2060 a         3261 c         3525 g         1921 t
ORIGIN      
        1 gaactccttg acatcggagc cggcgcagaa cgctctatcg cccgcgccgg tgacgacgac
       61 cgcgcggacg gcttcgtctc cttcgagttc ttcaagggcc tcaatcagct gctccgtcat
      121 ctccagcgtg accagattga gcggcgggtt gttcagggta agcttcgcga tgccttcgcc
      181 gggccgctcc acgagcacag gtccttcggc caaggtctag tacaccccca taagcccgga
      241 aagccacagc gagaggctga ggaagattgt gagtcgaaac agttagtttt cacacaacag
      301 aatctttact cactccacgg aaagcatagg gaaaggatgg gacgtggtca agcggttagg
      361 atataagggg aggtgcgaac cgtaattcag gcagtggaag aggccgcccg cggctcttga
      421 cctagccgcg tcgtccgagg gctgcgtcgg gttgctccct ggacgcgcct ggcagccctg
      481 cctcggccac gacgcgacgg atcttttctc tctccgcgtc ggggatgcgc cggagtgggg
      541 gacggacgtg ggcctcgtcg atcctgccca ggatagccag cgcctccttc atcctgttgt
      601 gcccgtcgag gaacggttcg gcgtaggtcg cctgcaccac ggggtaggcc ctgccggcca
      661 ccctgcgggc tcccttcagg tcgccgcggt tcacggcctc gaacatctcc acgtgcagat
      721 cggctatgac gctgccgtgc ccagagagga tgccgtcggc gccgatgcac agcgacgcca
      781 acagcgactt gctgaagcta gagagcagcg agatctcctt gtccagcgcc cccagctcgc
      841 gccagttccg ctcgtagacg ccgatgtcgt tggaccactc tttgacggcg atgacgttgt
      901 ctatctcgtc gcagatccta accaggctgt cggtcgggat gtgcagtccc gacgttaccg
      961 ggtagacgaa cactacgata ggcaggctca cggcttcggc gatctgagcg atgtgcccgt
     1021 aagccatctc gggcctgagc tgatgcccga agtcatagac cgtggatggg aacaccagaa
     1081 ggcaatcagc cccctgccgc tcggcggact tcgcgatcct ggctgccttg ctcgttccgt
     1141 cttcgtagac gccgcacacg acgggtatgg tcccgtccac ttcgtccagc gtcatctcca
     1201 gaacgcgctg ctgctcctcg aaggtcagcg tcgccacctc cgaagcgtgg gcgttcgtgg
     1261 tgaggccagc taccccaggt gtgtcgatca ggtagcgcaa atgcctgcgg tagttctcct
     1321 cgtcgatctc caactcggaa tcgaaagcca tcaagttggc gggtatgaca ccccttagaa
     1381 ccagctcagg cctcatagga aaacctctcc tcgctccgct cctggcgcac cggttactct
     1441 agcatccaga ccagtcactt tcacagagtc tcctgtctcg gagacccgcg ccttcgctct
     1501 ggagcgaaac caaagagctg acaagctgaa gtgctgactg gctgaaatgc ttgcaaagag
     1561 gtcttgaacc tctttgcact agtacgcgtc gacggtaccc acgtagcctg ggatgttttc
     1621 gatgggtgtc tcaacgacgg ccccgtagta ggtcgaggcg tggatcaccg tcccgtagcc
     1681 ggttgctatt ccgacgtgcg agatgccgta gccggcctcg tcgaagaaca caaggtcgcc
     1741 ggcttccccg taggagggcg tcccgtaagc gtactgggcg tccggggagt cgggcaggta
     1801 cacgccgagg tcggcgaaca cagccgaggt aaagcccgag caatccatgc cccaggcacc
     1861 atagggcgta ccgatgtagg tctgggcgtc gtctacgacc gcctgtcccg agtagccctg
     1921 ggcgtgcgct tcagaaggtg cccctgtcag tagtacgacc gaaaacaggg cacaagaaaa
     1981 caggacagca gcgagttgcc gcacgatgta tatccccctc caattatctt gcttgcacaa
     2041 cggcggtgga tgctaccaga gatttgagcg gtttcaagac ggatccggcg cagtaatggg
     2101 cctctcgcgg cgcgaaggca cccggtatca ggcaggtgct aaaccggaag acggtcgtac
     2161 atctcgtccg cgtacgcccg cgcgcctctt catgacccgc ccgcccgcga gggccgcacg
     2221 cccggtgcag acctcgggct gagagagctt gtggagtaag aaaagctggt cccgattgcc
     2281 caaaaaccat cccaggtgct agcaccagac ggtgctattc cgtgcagtgc cttcaccagg
     2341 atcgcctctt aggatacccg gcaacggcgg acgaacctgc gcgcaaacga ccgtggaaat
     2401 ccaacatggc cgtacacttt gtcgtctcga tgccaacagt tggcctctga atgcgtctta
     2461 tattgtaaaa gcgcgctcca caacggtcgg gtaagaggtg aggagcagtt ccacagagga
     2521 ggacctgaga gatgtacgag atgatggatc cgcaagtctc gagacaacgc cgtgaggaga
     2581 tgctgcacca ggcggagcgg ggtcgggtgg caggtgcttt gccggcggat tgcaagagac
     2641 gccctgatcg gacgctggca tgggaactgc agagggtagc tggcctgctg ctaaagctgc
     2701 tgaggagatc gaagaagatg ttgatggccc gaaagagcac gccggacggg atgaagcgag
     2761 cgcggaggaa gaagtatggc tgaacgagaa ggtatggtcg gaggcgaggg cctcgggtcg
     2821 ggcgcgatct ccaatgtggg agtactggac ttgaccggca tgaagtcgac cgaagagtta
     2881 gccggcatcg ggagcataag ccgggtcggc atcgtcctgg tccccgaaac cttgatgtcg
     2941 gggcttctgg ccgttccgat ggagaaggta ggagctactg ttcccgttcc cgcaggtgag
     3001 aacgtaaagc tggtgacggg tcagatgaag acaaccggtg aggcgctggc cagcgctggt
     3061 cgagaggagg atgtgcttgt ggtggctggc cagttgttgg tcaccacccc ggtcgaacgc
     3121 gtgggctacg gtgagatgat cgtcgccgga caagtctacg ccccccaggg gagcgaaggc
     3181 gctctgaccg cagggatctc ccgtcttatt ggtcaggtgt tctactactc cgggaagccc
     3241 aggttcttcg tcggcagcga gcgattcggc aagggtttct tcgaggtgtt ggagggaccc
     3301 atggctatga tcctggtcgg agacttcgcc atcgagcgcg gcgtgcccgc tgatctcctc
     3361 aaggagaaga tctcccagat cctgctcgcc ggcaacctac aagcgccaca ggaactgcta
     3421 ccgctcgtcc aggttctgac ggtggagaaa gcgggaaaca tctcggccct cgaggaaggc
     3481 gacgaagatg gggagcggac cgagccgtag ttgcgctgac cttggaggca gagcgaagta
     3541 tggtagacag gacgccagac ggggccacct ctggcctggt gaggtgcttc aaccgcatct
     3601 acgatgcaaa ccatcgcgcc ctccacgcct acttcctggg ccggaccagc gatccggagg
     3661 ccgccctgga cctctcgcag gaagcattct tgagggtttg gcgaaacgta tccaaactcc
     3721 aggaactgac gccgcaacgc cagcgctact ggctgtacac cgtagccaag aatctggtgg
     3781 cggatctcta tcgcggctct gcgtcccaaa gagctgcccg acagagcgta gtcgcaagac
     3841 tccgatcggg tgggaaccac gaggaaggcc cgcaggccgc gctagaaagc cgggagcggc
     3901 tggaggccgt cgatagggcc atgaacgaat tgccagagga tctgcgcacc gccctacttc
     3961 tgcaggtgat gggtcggctg tcgagctccc agatcggcga ggcgctggag gtgccagccg
     4021 gaacggttcg ctaccgcatc gcgatggcgc gcaaacgcct ggccgagctg ctgcgcctcg
     4081 acgaacacct cggaacgaaa ggacgaacat gaccgatcac aatctggatc ttccctccga
     4141 cacagacgct cgtctggacc ggttgctaga ggagtgggtg gaacagcgcg ggctcactaa
     4201 ggcccgaacc gaacagctcc gccaggcggt actcgcgatg catgagctgg atgccgcgac
     4261 acccgctttg gcggcggcgt cactggatca gggagcagaa tggtggagta gcctgttcac
     4321 gccccttctc accgcgctct cccaggcggc aactaccggg tacctgagcg taggtacggg
     4381 gatggaagcc gagccagacc ccagtgtcgg ctacagagcg tatatgcgcc tcgcctgagc
     4441 agaggcgctc ctctgacagc ggtttcctga aggcctaggt gctggtctcc tcgggccagc
     4501 gtccgccgtt gtaccagtag gagcggatcg cttccagcgg tcgtcccttg gtctcggggg
     4561 cgagtacgta gacgaaggcg aacgagacca gtgccagggc gaagaagatc ccgaaggtca
     4621 cagagcctcc aagggcgccg agggcgccca ggaacacgac tccgacgatg aagttggcga
     4681 acaggtcggc cgtcagcatg gccgagccgc cctgggtgcg caggcgcacg gggaagctct
     4741 cggacgcgta gacccagacc agcgaaccgt agccaaagtt gaagcccacg gtgaagagca
     4801 ggatacctag caaggtgaac gtcgccgggg agttcagcgc gtaggccagg acgaggatag
     4861 cgttggcggc cgccatagtg ccgatcccgg tcagaagcgt gggccgcctt ccccacctgt
     4921 ccacgaccag gaaggcgatg acctcggcga tcacgcctgc aacctggacg aagccggtcg
     4981 cgatgatcgc gcccgtcggg ctcgtgacgc cgacctcctg gatgatcgtc gggctgtagt
     5041 agacgatggc gttgatacca gtgatctgta ccaggaagcc caggccaacg acgaagatgc
     5101 cggccttacg gaagcgtccc tggaagagct cagcgaagct tcccttctcc tcgtagctaa
     5161 ggtcatgctc gatcctctgg acttcctcat cggggtccat gtcgggttcg accctgcgca
     5221 ggaggtcggc agcctcctcg cgccggccgt tcatcagaag ccagcgagcg gtgtctggca
     5281 gacgcacgat gaggatcaac acgataacgg cagggatggc tgagagggag agtatgagac
     5341 tccagctctc ggttggcgcg agggccacgc cgacgaagta ggcgaccgcg atacccgagg
     5401 tggtcgctat ctggaaggtg acgagcatcg agcccctgac gctgacgggt gcggactcgg
     5461 caatgtacgc tggcgccacg actatggaga tgccgatggc caggccgagg aggaacctga
     5521 cgacggtaag gaaccactcg ttgggggcca gtccctgcag gccggcgaac gccgcgtagc
     5581 ccagcgcgac aagcaccagg gagcgcttgc gccctatggt gttggagatg cgccccgcga
     5641 agagcgcccc gaacaactga ccgagcacca cggctgaggt aaccaccgag gtcatgaaag
     5701 tggtaaggtc gaaggcgggg acgaggaaga ggagtgcgct cgcgatagag cccaggtcgt
     5761 agccgtagat caggtcgatg atcgcggccg tgatggcaat gaggatgttt agcctcgata
     5821 cgcttcgcac gcgcgctgcc ccttcttaca cagcaaacct tccttcccca ggggtcccat
     5881 ccgagatgct ggcatagtag catagtgggc gaggacctag gggccccaca gaatgcggaa
     5941 atggtatcga gacctggggg agcgtgaaac gtttgcacgt ctacagtgat ccggccacgg
     6001 agcgccacgt caatgacctc gccggcgtgg aatccccgaa gaggtttccc gctgctctcg
     6061 caggggtcga aagggctgag caaagcggcg tactcgtgga gcggcgcccg tgcagtcccg
     6121 cggagagaga tgccctcact gctgtccacg acccggctta cctggacgat ctgcaggaaa
     6181 tgtccgcggc gggaggaggc tacctcagcc ttgacacggc cgttggcgcg ggttcctggg
     6241 aggcggcttt gctagcgagc ggggccgcct gctcggcggt cgagagcgtc ctcgccggcg
     6301 agacggcctt cgccgtcgcc cggcctcccg gacaccacgc agggagggac tacgggatgg
     6361 gattctgcct cacgaaccac gcagccgtcg cagccgggca cgccctttcg agagggctgg
     6421 aaagggtcgc catcctggac tgggacgtgc accacggcaa cggtacccag gatatcttct
     6481 acggtgacgg tagggtactc tacctctcgg tgcatcagag tccattctac cctgggtcgg
     6541 gcgaggcaag tgaggtgggg aaaggagaag ggcttggctt cacggtgaac ttgccgctgc
     6601 ctgctggctc ggaggaagac ctctatgcgg ctgctttctc cggggttttc ctgcccgtgc
     6661 tgcgggaatt cgggccccag gtgatcctga tctccgcggg tttcgacgcc cacgtagacg
     6721 atcttctagg gggcatggcg ctggggagtg actctttcgg acgcttcgcc gccctgatct
     6781 cagacctctc ccgggagatc gaggccgcgc cccctgcgtt cgtcctcgag ggaggctaca
     6841 acctcgacgc cctgaccgag agcgtcgccg ccaccatgca gggggtcggg gagagagctc
     6901 ccgagtggga gtatgcgggc gggtcgaggc acattcagaa ggcccgcgag gccctggcgc
     6961 cgttctggcg gagcttgcgc tgaggctatg aggatcttcg gctttccgcc cttcgaggat
     7021 cgcagggacg cgggccgccg gcttgccggg aagctgtccc gcttcaagga cgagcggacc
     7081 gtggtcttcg ccctgccaag aggaggggtg cctgtggggt atgagatctc acgcgccctc
     7141 gaggcgccgc tggacgtgtt cgtctcgcgc aagctagggg cgccagggca gccagagttc
     7201 ggcatcgggg ccgtcgcggc cggcggtgtg cgcgtcctca accaggacgt ggtcaggcgt
     7261 ctcggcatcc ccgacgaata cgtccaagag attacggcgc aagagctcgc ggaggtaggg
     7321 cgcagactgc gctacttcag gggccagagg ccagagacgg aagtggtggg gcggaccgcg
     7381 atcctggtcg acgacggcct cgctaccggg gtgacggccc gcgccgcagt acaggccctg
     7441 cgtttgcgga aaccgagacg attggtcctg gcagcgcccg tatgcgcagc ccagaccgcc
     7501 gatctctttc tgcgcatggt ggacgatctg gtctgcctgg agtctccttc tgacctgggg
     7561 gccataggct tctggtacag gaacttcgag cagacctcgg acgaggaggt cgttgaactg
     7621 ctagaaagag ccaggagcga acgaggtgaa aaggggagcc cgtagaacca tcctgagccg
     7681 caacaaccgg ccggtgggag acgggcccaa gaccggtaca attacactag aattccgagt
     7741 tctaaagagg tgagagacat gtgcggcagg tatacgcttt ctaccccggc aggcaggctg
     7801 gccgaggagt tccaattgga cgaagtttcc gtggaccttg cgccgagcta caacgttgcg
     7861 cccacgcagg aggtagctgc ggttctggag gaggaaggga agcggcgtct ggagatgctc
     7921 aggtgggggc taattccttc gtgggccgac gaccccgaga tcggggcgcg catgatcaac
     7981 gcccgcagcg agacggccgc agagaagccc tcttttcgca gtgccttccg caggcggcgg
     8041 tgcctgatcg cggcggacgg attctacgag tggaagcgcg agaacggggg caaacagccc
     8101 tactacttcc acatgcagga aggccgtccc atcgcgttcg ccgggctctg ggagagctgg
     8161 gacaagggcg gcggggctct ccgctcgtgc actattctga ccaccagggc caactccgtg
     8221 ctcgaaggga tccacgacag gatgcccgtg atactgcctt ccggcgctta cgacctctgg
     8281 ctcgatcccg acgcggacag ggagcagctc gccgacttgc tgattcccta tcccggcgac
     8341 gacctcgaga cctacccggt gagccgcttc gtcaacagcc cgcgcaacaa cgacgagcgc
     8401 tgcatagagc cggccgcttg agccccgaga tcagggccta cgttagggaa gccacgagaa
     8461 atggagcccg tcgtggcgct cgagctggcc ctgatctccc acggattttg ccgccccgga
     8521 atctgagcgt tcccagcacc gcctccaccg cctgaatcga gaggagcgcc ttgttggcga
     8581 acgtagcagt tgctgagggc ggacgtgcgg tccttcagaa actgatccag gagcccttgt
     8641 acttgaaccc ctgtctttcg acccagccga atctgtagac gccattgtcg ccacggtagt
     8701 agtggtggtc gactaccttg agcccagcgc ccccgtacgg cgccccgtcg gaggtgtagg
     8761 tggggtagcg gtagtaggcc tggtgcaggt gtacaggccc gaccgggatg tcgccgcctc
     8821 ccgtcctgcc ggcgaagccg ataacggtgt tcctgtcgac tactgctccc ctcctgatcc
     8881 cgggagcgac tctgctcagg tgccccgaga cgttcacgta cttgccgttg gcggctttga
     8941 tcgagacgaa gataccgtag tctttgtgcg tgatgttgcg gcccgcgaag gtcaccgtgc
     9001 cacgcctgaa gggcgagaag atagcatctc cggggtcgag gaggtagtcg acggcgaact
     9061 tgtcgttggt gtaatgtcgc ctgtcacatc gaccgtactc gagcctgccg tgggtgtact
     9121 catcccagta gttgggtctg ccgatgatgg tccaacccgt agggacgcta tcttcggggt
     9181 tggcgttggc cgtggaccca tactgcgtct ggaagaagaa gtcgggatca gcctgccagc
     9241 aaccttcttc gattggagtc tcctctccca gcgacgaggc tgcgaaggtc gcctccccct
     9301 ccgtagtcgc ctcatgaatc gtccgctccc gctcggcgag ctcaggatcg cccgccccat
     9361 agagggtttt cacagaattg gcgaccggta gcccgagtga gctgaccggc ctcgaggggg
     9421 gttcgaagcg taaagtcgag agcagctcct tggcgtctga acctgatgtc ttcccgtaga
     9481 cgttgatctg gtacacccgg ccgttcaccg gcacgtagac ctctgtggag ggcgtgctgc
     9541 ccgggatcgg cccgacagcc acgcctctgt gtcccttctc ggcgacgctg acctgctcgc
     9601 gagtcatggg gaggttgggg taggcacgga gcttctccag gaccgtcgcc ctgatctgtc
     9661 ctggccgaag accgtaggcc agcgcgacgc gcaccgccgg cgtcccgccg tggtcgtgcg
     9721 gagcgcctga gtcaggcctc cacaacgtga agccgtaggt ctggccgtag ctgtaccgct
     9781 cgcgctcgac gaaccatttg acgggatggg ggatcgaagc cccgaccacc tcaccttccg
     9841 tcctctcgac cggccccttg ctctcccgga gagcctcagc cgaggagacg ctatttcctg
     9901 cgatcgacaa cgcgcacatc gccccaacga tcccaagcaa gacggcaaga cctctcaggc
     9961 ccccacaatg tgtgaggttg aacctagaaa cgcgccgcac cgtgtccccc tccggtagtt
    10021 attcttccct tgttcgtagg gcacgcatat taagtagctt atggaccgga gtcaatagct
    10081 gctctagagt tcctgagcac gtacatgaca gcctctagaa cgcgagcctg cccgttcagc
    10141 aaacaaaaag ctgccaagct ggagtgctgg tcagcccggt atgccagcca cggccgcttc
    10201 tgtctcctcg tccgagtgct ccaggttgac cagctctccg gcgaggtact gttggtaggc
    10261 cgagaagtca aagtgaccgt ggccgcagag gttgaagagg atggtctgct gctctcccgc
    10321 ctctttggcc tcgagtgcca gatctatggt tgctttgatg gcgtgcgtca cctcgggagc
    10381 gggcacgatc ccttccgtcc tggcgaaagt aaccccggcc tcgaaggtgg ggttctgggc
    10441 gtaggcccgc gcctcgatga gtccctcgtg tgccagcgcc gagactatgg gggagtcgcc
    10501 gtggtaacgc agtccgccgg cgtggatgcc gctcggaacg aaagagtgcc cgagcgtgta
    10561 catcttcatc atcggggttg tgcctgcggt gtcgccgaag tcgtaagtgt agcggccctt
    10621 cgtaagcgtg gggcacgagg ccggctcgac ggccatgatg cgcgtgctct tcccgttctt
    10681 caggttctcg cggacgaaag ggaaggctac gccgccgaag ttcgagccgc ctccgacgca
    10741 gccgatgacc acgtcggggt actcgtc
//
LOCUS       DASTKM010000116         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_378977, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000116 DASTKM010000000
VERSION     DASTKM010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378977"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFQ10_05030"
     CDS             <1..238
                     /locus_tag="VFQ10_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964736.1"
                     /translation="SFNWAEHVDLETAISQQEALTALLEANQLVVKTAVLEEIADGWP
                     AKAQRRTRESHTGTIWLSEAGLSLSRVTPVPKAD"
     gene            408..674
                     /locus_tag="VFQ10_05035"
     CDS             408..674
                     /locus_tag="VFQ10_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964737.1"
                     /translation="MTHGGSEVLPVFSFREEAEMYLLMERLGSDWQIRETDVGELASV
                     LSEQCASVRSVALDPLPSTLAEEVAELVSLSREHFVSRAVRRIA"
     gene            1462..2292
                     /locus_tag="VFQ10_05040"
     CDS             1462..2292
                     /locus_tag="VFQ10_05040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9964738.1"
                     /translation="MDSVGLTSRWMAAARARESQRLDRLFDDPLAAALAGPEGFAWLD
                     ELERAQPWGEPALYVVVRTRFFDEFLLRATWEAGARQVILLAAGMDARAFRLGWPPGT
                     RLYELDRPEIIDAKDAVASGADAQLTCERQTIGVDLEHPSWSDALLSAEYEAQEWSVW
                     LAEGLLFYMTEAATRELLRGASVLAAPHSQLGADLVNRHLLGSPVMWPLLEVLSRRGA
                     PGRFGTNDPEPLFAEHGWEAQVTQPGEREANYGRWPYPVAPRRMPGVPRLYLIRAQRS
                     "
     gene            2513..>2637
                     /locus_tag="VFQ10_05045"
     CDS             2513..>2637
                     /locus_tag="VFQ10_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET9964739.1"
                     /translation="MPQEHVVSIDPSKPLSEEPGTGHNRWHEDIEPVVEVDPGDTV"
BASE COUNT          539 a          797 c          855 g          446 t
ORIGIN      
        1 gagcttcaac tgggccgaac acgtcgacct cgagacggcg atcagccaac aggaggcgct
       61 caccgcactg ctcgaagcta accagctcgt ggtgaagacc gctgtgctcg aggagatagc
      121 tgacggatgg ccagcgaaag cgcagagacg gacacgggaa tcccacacgg gcacgatatg
      181 gctctcagag gcgggactgt ccttaagtcg cgtgacgccg gtaccgaaag cagactgaaa
      241 aagctcagct gaatcgacaa ccacagcggc tggcaatgag ataggggaaa gcaggaacta
      301 tggcggaaag cttaaggagt aaaccgacag tctcctctac tttgcgaacc cattcagcgt
      361 ggtggctgct cgcaaggcac agcgatagcc aaagggaagt gcttaccttg acccacggcg
      421 ggtctgaggt gctacccgtc ttcagtttca gggaagaggc cgagatgtac ttactgatgg
      481 aaagattggg cagcgactgg cagatcaggg agacggacgt tggagagctc gcgtcggtgc
      541 tctccgaaca atgtgcaagc gtgagaagcg tggctctcga cccgttaccg agcacgttgg
      601 ccgaggaggt ggcggagctc gtgagcttaa gccgagaaca tttcgtgagt cgagcggtgc
      661 gcagaatagc gtagcccaga ataggtgcgc cgtcggcgct agcgctcacc tgctcaggga
      721 tttctcctgt cctcgtggtg atcagagggt gacgtacatc tcgaccctgc cctccggccc
      781 tacaatcgct cgaaacgtca ggctccgcgt tgtcccgatt tgcgtgagcc cactcgaagg
      841 cggtagcaat ccgtccccgc cgacgccttc ccgcgcagcc tcgagagatc ggatggtcag
      901 acatctcacc ggtcctctca caccgtctat gcgccttgac ctataccctc ccggctatta
      961 ccatggttgt agcagaagtt ctttttggtc ttgcctcagc ggcaacggtc ctgaaggaac
     1021 tggttagccg caaccacgag ggtaacgtga gcgtttacac tatctataag gaaactcact
     1081 cttcggacga tcccgcaaca tgccaaaggc attatgcagg atggaccgct gacaaggacg
     1141 gcgcaatacg gcgcgccaac gagatctacg agggaatgga tagcacaaaa gcggtattgg
     1201 taattggtaa cggccggaca ggggtcgaag tcgtctaccg ggtaggcgat gaaaactagg
     1261 ggtgtcatag agttatccgc ttgcacgctg gagtacgctt gcggtgagtg cctggagtcc
     1321 gaaggcgacg aggcgtcgtt cgagcgggcg cgagagcttc tctaggtccg cgcgcacccc
     1381 gagaagggga tccaagtcga gcaggtccgc aaccgcatat aggccaaagc ccacgaggcg
     1441 gaatgacagg aaaccccaac gatggactcg gtgggcctga cttcgcggtg gatggccgcg
     1501 gcgcgggccc gcgagagcca gcgtctcgac cgcctcttcg acgacccgct cgccgcggcc
     1561 ctcgccggtc ccgagggctt cgcctggctg gacgaattgg aacgcgccca gccgtggggt
     1621 gagccggcgc tgtacgtcgt cgtccgcacc cgtttcttcg acgaattctt gctgcgagcc
     1681 acctgggaag ctggagcgag gcaggtcatc ctcctagcgg ccggcatgga cgctcgggcc
     1741 ttccgcctgg gctggccgcc cgggacccgg ctctatgagc tcgatcgacc cgagatcatc
     1801 gacgccaagg acgccgtcgc ctccggagcc gatgctcaac tcacctgcga gcggcagacg
     1861 atcggtgtgg atctggagca cccttcttgg tccgacgcgc tgttgagcgc cgaatacgaa
     1921 gcccaggagt ggtcggtgtg gctagcagag gggctcctct tctacatgac cgaggccgcc
     1981 acccgcgagc tcctcagagg cgcgagcgtg ctggcggcac cccacagcca gctcggcgcg
     2041 gatctcgtca acaggcatct cctgggctcc ccggtgatgt ggccattgtt agaggtcctc
     2101 tcccgtcgcg gtgctcccgg ccgcttcggc accaacgacc cggagcccct ctttgccgag
     2161 cacgggtggg aggcgcaggt aacccaaccc ggcgagagag aagcgaacta tgggcggtgg
     2221 ccctacccgg tggcgcctcg gcggatgccg ggcgtccctc ggctctatct cattagagcg
     2281 cagcgctcgt gagctgccgc cccagcgttg ctcccggctc gcagagacgg gagtgcgacc
     2341 cgcccccgct tctacctacc cgcagccctc ccccgcgcag ccgaatagga ccggggggta
     2401 ggatgctagg gcgagccgac cctgcgagtg ggtggcgcgg gactaacctt tgttagaaca
     2461 tgagtgcagg ggaaggaagc acactgcgac ccagggcgcg aggaggaaag cgatgcccca
     2521 agagcatgtc gtgagcatag atccatccaa accgcttagc gaggagccgg ggacgggcca
     2581 caatcgctgg cacgaggaca tagagccggt ggtggaggta gatcctggcg acaccgt
//
LOCUS       DASTKM010000117         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_555456, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000117 DASTKM010000000
VERSION     DASTKM010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555456"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..337
                     /locus_tag="VFQ10_05050"
                     /pseudo
     CDS             35..337
                     /locus_tag="VFQ10_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            334..1638
                     /locus_tag="VFQ10_05055"
     CDS             334..1638
                     /locus_tag="VFQ10_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HET9964740.1"
                     /translation="MTERRTPLYDFHFRSARNIVKGGGDFMFPTSYTSPVEEHLNVRR
                     NVGMQDLSTMGEVDIKGPGSERLVRRLLVNEVNDMEPGQLRYSTMCNEEGGIVDDVTV
                     YKFGDEHFMIVTSSGPRLKTYRWISEHAEGASAYATDVTAALALPVVQGPRSRTFLKT
                     VVEGVDLDALRFFRFAQCRIGEVELIISRSGYTGELGYELYTPADQAGPLWDFLLEKG
                     REFDLKPYGVEAMQSLRIEKSLPLYGPDISEEDTPFHVGLARWIRLEKRDLIGREALL
                     RIQERGLDRRWVGLTLESEVPAAAGAKVYSVGDVATFREVIETGAEAGEYEDAVMPGD
                     RQIGRVTSSAMGRSVGKMLAMAYVETAHSWPGNNLIVEVNGRPIRAKVAQTPFFDPEN
                     ARIRSEPAEEGRRSEPTPRLVATTNSGSNVPARQYTNEGPGS"
     gene            1635..2900
                     /gene="solA"
                     /locus_tag="VFQ10_05060"
     CDS             1635..2900
                     /gene="solA"
                     /locus_tag="VFQ10_05060"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HET9964741.1"
                     /translation="MSVTYSGRYDAIVVGVGGMGSATSYYLARRGKRVLGLERFGIPH
                     SMGSSHGHTRIIRLAYYEHPSYVLLLKRAYELWREIQRIASERLLHITGSIDAGPEDS
                     WVFKGSWESCRLHDLPHEVLTGAELHRRYRGYHLPADHLALLQPEGGFLTPEKCIVSY
                     VMAAQAFGAEIHAHERVFEWRPLEGGVRVRTDRGVYEADKLVITAGAWDGELLDVLEV
                     LAVPERQVLAWLQPTRLEYFRPNAFPVFNLLVDEGRFYGFPVHGVPGFKFGKYHHLEE
                     KVDPETVDREAYDYDERLLRDFAERYFPDGCGPTMDLQTCMFTNTPDNNFIIDMHPEY
                     DQVSFASPCSGHGYKFASVIGEIMADLADTGITRHDVTLFRLDRLTRPSRGLPGRAHP
                     TMGTTDREAHQQNGRPRSFPRVSEIRPFW"
     gene            3095..>3726
                     /locus_tag="VFQ10_05065"
     CDS             3095..>3726
                     /locus_tag="VFQ10_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET9964742.1"
                     /translation="MQSVDRAITVMELLSRRGWSGVTEVANELGINKSTAYRLLTTLR
                     DRGLVEQDAATEKYRLGFGLITLASSVTADLDVARCSRPVCDWLAERTRETVTVAVLE
                     GDDAVILYQSNSRSSALSVDWSGMHTPLHATAAGKIFLHYMPEDQRRRILKRSLQRYT
                     ENTIVNPKALRQELQRDHSRDYCYTIEELEVGLNAIAAPVRCSDGVVVAAV"
BASE COUNT          731 a         1132 c         1159 g          704 t
ORIGIN      
        1 atgggtgccc gcgtcataga aagaacacgt ccgcgagggg gttattgcca tagacataga
       61 aataaatccg gtgagggacc cccggaccgg caaacagggt ttcgtgggtg acctggattt
      121 cttcggggtc gcggctaggg ctgaggcgct ctcgccggtt ccgggaggtg tcggcccgat
      181 caccgacgtg tggctcctca agaactccac cgatgccgcc cggatctcca ctcgcgccga
      241 ggcagccctg cgctcgctgg agccggcgca cgcaggtgct gtcgtaggaa cttgtacaat
      301 atctgtggaa tctgtggggg aaaggatcat tcgatgaccg agagaagaac accgctgtat
      361 gacttccatt tccgttcggc gaggaacata gtcaaaggtg gcggagactt catgttcccg
      421 acctcctaca cctcgccggt ggaggagcac ctgaacgtgc gcaggaacgt cgggatgcag
      481 gacctctcta cgatgggtga ggtggacatc aaggggcctg gctccgagag gttggtgagg
      541 cgcctgctcg tgaacgaggt caacgacatg gagccggggc aactaagata ctccacgatg
      601 tgcaacgagg agggcggcat cgtggacgac gtcaccgtct acaagttcgg cgacgagcac
      661 ttcatgatcg tcaccagctc cgggccgcgg ctgaagacgt atcgctggat ctccgaacac
      721 gctgagggcg ccagcgccta cgctaccgat gtgacagccg ctctcgccct accggtggtg
      781 caggggccgc gatcccggac gttcctcaag accgtcgtcg agggtgtgga tctcgatgct
      841 ctacgctttt tccgcttcgc tcagtgccgc atcggcgaag tggagctcat tatctcccgc
      901 tccggataca ccggggagct cggttacgag ttgtacacac cggcggatca ggccggccct
      961 ctctgggact tcctactgga gaaaggtcgc gagtttgacc tcaagcccta cggcgtcgag
     1021 gcgatgcaat ctctacgcat tgagaaaagc ctgccccttt acggccctga cataagtgaa
     1081 gaagataccc ccttccacgt ggggctggct cgttggattc gtctcgagaa acgtgatctt
     1141 atcgggcgtg aggccttact gcggattcaa gagagaggtc tcgataggcg ttgggtcggt
     1201 ctcaccttag agagcgaggt gccggccgct gccggagcga aagtctacag cgttggcgac
     1261 gtagcgacct tccgcgaggt catagagact ggtgccgagg ccggtgagta cgaggacgcg
     1321 gtcatgcccg gggaccggca gatcggacgc gtcacctcca gcgccatggg acgcagcgtc
     1381 ggcaagatgc tcgcgatggc ctacgtggag accgcccact cctggccggg caacaacctg
     1441 atcgtcgagg tcaacggtcg ccccatccgc gctaaggtcg cccagacccc gttcttcgac
     1501 ccggagaacg cccgcatacg gtccgagccc gcggaggagg ggcggcgttc cgagcccacg
     1561 cctcgcctgg tggctacgac taactctggc tcgaacgtcc cggcgaggca atatacgaac
     1621 gagggccctg gctcgtgagc gtcacctact caggccgtta cgatgctatc gtcgtaggtg
     1681 tcggggggat gggtagcgcc acctcctact acctcgctcg tcgcggcaag cgtgtgctcg
     1741 ggctcgagcg gttcggcatc ccgcactcca tgggctcctc gcacggccac acccgtatca
     1801 tccggttggc gtattacgag cacccctcat acgtgttgct cctcaagcgt gcctacgagc
     1861 tgtggcggga gatccagcgc atagcaagcg agcggctcct gcacatcacc ggctcgatag
     1921 acgctggccc tgaggactcc tgggtcttca agggctcgtg ggagtcctgt aggctccacg
     1981 acctgccgca tgaggttctc accggcgccg aactgcaccg ccgctaccgc ggctaccacc
     2041 tacccgcaga ccacctcgcg ctcttacagc ccgagggcgg cttcctcacg cctgagaagt
     2101 gcatcgtctc ctacgtgatg gccgcacagg cttttggagc cgagatccac gcccacgaac
     2161 gggtgtttga gtggcggccg ttagaaggcg gggtgcgtgt tcgcaccgac cggggcgtct
     2221 acgaagcgga caagcttgtc attaccgccg gtgcttggga cggcgagctg ctcgatgtgc
     2281 tcgaagtgct tgcggtgccc gagcggcagg tcctggcttg gttgcagccg acccgtctcg
     2341 agtacttccg gcccaatgcc tttcccgtct tcaatcttct ggtggacgag gggcgcttct
     2401 acggcttccc cgtgcacggt gttccgggct tcaagtttgg taagtaccac cacctggagg
     2461 agaaagtcga tcccgagact gtcgaccgcg aggcgtacga ctacgacgag cggctcttgc
     2521 gggacttcgc ggagcgttac ttccctgacg gctgcggccc gacgatggac ctgcagacct
     2581 gcatgttcac caacactccg gacaacaact tcatcataga tatgcacccc gagtacgatc
     2641 aggtttcctt cgcctcgccc tgctccgggc acggttacaa gttcgccagc gtgataggcg
     2701 agatcatggc tgatctagca gacaccggta tcacgcgcca cgacgtgacc ctatttcgcc
     2761 tcgaccgact gacacgtcct tcccgagggc tccctggcag ggcgcatccg acgatgggga
     2821 caaccgaccg cgaggcccac cagcaaaacg gcagacctcg ctccttccca cgggtgtccg
     2881 agatcagacc gttctggtag ccagtgcagc gtggcatttc tccccgcgtc gcaaagaact
     2941 gggttcctca tgtcgacgac aacacacctc tgacggtggt agttgcgaag tgatgaaaac
     3001 gagtgacggg caccgagaat accagtatca agccagaatt taatgttagg agtagactac
     3061 acatatggaa accacaaaaa gtaggaacaa gtttgtgcag tcggtagacc gcgccatcac
     3121 cgtgatggag cttctttcgc gccgggggtg gtccggcgtt acagaggtgg ctaacgaatt
     3181 gggaatcaac aagtccaccg cctaccgact gctcactaca ctgagggatc ggggattggt
     3241 cgagcaggac gccgcgaccg agaagtaccg gctgggtttc gggctgatca cgctcgcaag
     3301 ctcggtgaca gcggacctgg acgtcgcgcg ctgctcccgg ccggtctgtg attggctggc
     3361 cgaacggacg cgggagacgg taaccgtcgc cgtactggag ggggacgacg ctgttatcct
     3421 ctaccagagc aattcgagat cttcggcctt gagtgtggac tggtcgggta tgcacactcc
     3481 gctgcatgcc actgcggcgg gtaagatctt tctgcactac atgcctgaag accagcggcg
     3541 tcgcattctc aagagatcgc tgcaacgcta taccgagaat acgatcgtaa atcccaaagc
     3601 cctccgacag gagctacagc gcgaccacag tagagattac tgctacacca ttgaggagct
     3661 ggaggttggg ctcaacgcga tagccgcacc ggtccgctgc tccgacggcg tagtggtagc
     3721 cgcggt
//
LOCUS       DASTKM010000118        19537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1085156, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000118 DASTKM010000000
VERSION     DASTKM010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 19537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19537
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085156"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFQ10_05070"
     CDS             <1..396
                     /locus_tag="VFQ10_05070"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HET9964743.1"
                     /translation="FLPLPATTYAEMLSERLRKHGTRCYLINTGWSGGPYGVGSRIDI
                     AATRQMVSSVIGGKLDAAETRKDPFFGLNVPVAVPGVDGSVLNPRGTWDDGDAYDEQA
                     RKLADLFRENFSRFESQVSEEVRKAGPHF"
     gene            745..1269
                     /locus_tag="VFQ10_05075"
     CDS             745..1269
                     /locus_tag="VFQ10_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964744.1"
                     /translation="MAYTDDLREHEQVLLRLLDEMGRSLARQERLLEEIENRHVQMRN
                     SWIQEGADGPFVLPDQQALADELQRELERGRDQELELHEILDVLKRRCELEDRLLDEL
                     ESHLPREHRDQLPLELVDCFERRDRLLGDVELKSFREKRDRLAETMQLLPDLEGLLFE
                     AVERHLVRLHREGG"
     gene            complement(1281..1748)
                     /locus_tag="VFQ10_05080"
     CDS             complement(1281..1748)
                     /locus_tag="VFQ10_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9964745.1"
                     /translation="MRELREQAGLSQEELSLMVGQSGTWAEEMERESRVHVLEIIVQK
                     LTRIFGVEISDLSTFPAPPLPTVLEEPGLAVEKLRALREEASLSQEQLDELAELEPGS
                     IARAESVDPASEEIHASVDTIWKLAMALKVSPTILTKTRGDLHQIEPRRPVDS"
     gene            complement(1832..2080)
                     /locus_tag="VFQ10_05085"
     CDS             complement(1832..2080)
                     /locus_tag="VFQ10_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9964746.1"
                     /translation="MKVDGAKLAAARERVFMSQDELAESIGMNPVVVIRLETADRTEI
                     RENLGEDLMQILFVGRSELTSYPDPPEPTLEGPSDIAD"
     gene            2262..2405
                     /locus_tag="VFQ10_05090"
     CDS             2262..2405
                     /locus_tag="VFQ10_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964747.1"
                     /translation="MFREERAWTLADLQFLSYGGKDPTQALLAIIRIDTDHVIGPAKR
                     SVS"
     gene            complement(2430..3617)
                     /locus_tag="VFQ10_05095"
     CDS             complement(2430..3617)
                     /locus_tag="VFQ10_05095"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET9964748.1"
                     /translation="MKVNKWTRRHTNAEDGNAPPEAVPDSGDGGEIHQPPDGLSEAGI
                     REALRDVRDPEIGRDLISLNMVRGVKIEGGYVAVGIALTTPGCPMKHRITTDITDRLK
                     MIEGVEEVDVDFGVMTDEDRQNLLTSLHGGPSELAPAFRDESKTRIIAVVSGKGGVGK
                     STVAVNLAAALDRAGHSVEILDADVHGSSIPVMLGSLDKPNVVGGVIFPIESNQGLKF
                     ISMGNFVNEGQAIIWRAPIVNKALTQLMRDVYWDEPDFIIVDMPPGTGDVALTVAQMI
                     PKAEALVVTTPQADAARVAVKAGKMAVQAHLKVAGVVENMAYAECPECGEQLQIFGGD
                     GGDRVAGELGSEVLGRIPIVPDATGEPGRSLFEADSTPARSFDEIAAALAATRVRRRI
                     KVL"
     gene            3806..4012
                     /locus_tag="VFQ10_05100"
     CDS             3806..4012
                     /locus_tag="VFQ10_05100"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HET9964749.1"
                     /translation="MARTIRCTEAQRTLDLEVDQNCDFVARAETEDEALAEFADHMQT
                     VHNWNEIFEPDLEKVRAQNVRNDV"
     gene            4035..4460
                     /locus_tag="VFQ10_05105"
     CDS             4035..4460
                     /locus_tag="VFQ10_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964750.1"
                     /translation="MDPSANSPPVPRFVFVAYYPEGPHAGPITIGLFGVQDGALLPIF
                     STREKAIDFAVRVLGTPPQNNENWDGVELPAEDLIQFLHTDASISYVAANPPPSGEVE
                     LIPTPEFVKRLEQEVGDADSPREDFRRSLVRSFVYGDVS"
     gene            4657..5829
                     /locus_tag="VFQ10_05110"
     CDS             4657..5829
                     /locus_tag="VFQ10_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HET9964751.1"
                     /translation="MPTGREAGDSIPGDQDTIEGRTSTEVRDEAKSVEFTYRGIFQKT
                     LAANLCKHVVMAARKRDWLGSTIQRYGDSPERNGVPAKQFAVIAPDTETLEGHMAKYE
                     PNELDVITVLDDTMCDGIESWAWYGRQPINVRLRRGGTLLVVSNRTPEELLKVIPQKP
                     WDWTLAILPGTPSLGGLWVYRDDGTDMRVLGAIARTRSDIFEYGEIEAVVSESGLGEK
                     YLTALKNGHDEVETHEVKAGEGAEDDYEPVKLPGWREMREAIIVEAVKLGERNPHYKT
                     SSDRTMRPVVNFDTCIKCRQCWIDCPDECFDISDEELHPVNYSYCTGCGICANVCPVE
                     DCIVMVNDLEFVEEGDKDVFPMWKEDKDGYHEWRDEKIARGRIDHDNFFNVDTWAK"
     gene            5846..7072
                     /locus_tag="VFQ10_05115"
     CDS             5846..7072
                     /locus_tag="VFQ10_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HET9964752.1"
                     /translation="MTTQDTTGRRQARQEEKLMTGTEAVAQAVRLADVEVTAAYPIRP
                     YDGVMQAVAKLIASGELDAEFIVAEGEHSQFEICKHASSVGARVFVGSSGVGWMYAME
                     SIAVTPSLRLPVVAMIGNRALDDPGAFGVEHNDALCVRDLGWQLLWVEDAQECFDATL
                     MAYRIGEDSRVSFPVGLGVDGAFITHSQALLQIPDQESVDAFLPPYDLGDRVLHPDNP
                     ISIAPQANEDWVMEIRKQADEAAKRVKDVIWEAHNDFKELFGRGGDNPFFEEYMTEDA
                     EYVLFGMGSLGLPAKVMVRRLREQGEKVGFIRLKWFRPFPDEELAEVLGRFKAVGVVD
                     RDYAYGSPQQGGVLFTDLRATLYAAERRPRMINFIGGLGGRELTPEMMDEMADITKRA
                     SEGEEVPVVSWIGVRG"
     gene            7135..8319
                     /locus_tag="VFQ10_05120"
     CDS             7135..8319
                     /locus_tag="VFQ10_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9964753.1"
                     /translation="MEAPGRALKLDPIKSIKKAPAEEYYQSGHRTCQGCLSALPMRLM
                     AKAAGPRTIVTGATGCMYVANTTYMSTPWVVPWMHTQLGAAGSSAVGTAAGLKALMRK
                     GKIKDEPINVIAFCGDGGGADMGLAAISAALTDVEYNLLIFLYDNESYANTDIQTSGQ
                     TPWGAVTTFSPPGKKHRIMQRRWKKNVPGMLAAGHPDTKYIASGTPAIPVDMMDKVRA
                     ALSAGGPTYMHVLDPCPKGWHYDPEHSNELGKLAIETGIYPLYEIKEGVCKLTGVTKQ
                     IAEGKRKRKPVREYLETQGRFAQFKNQDYAYFQKKVDEMWEQWVIPTVVAFSYGGPIN
                     ITGDMPKVRNRNVVRENASWAGIPGHAHEEFKEAADDPYEPWRDDNELGEYPWALPST
                     GQ"
     gene            complement(8402..8698)
                     /locus_tag="VFQ10_05125"
     CDS             complement(8402..8698)
                     /locus_tag="VFQ10_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HET9964754.1"
                     /translation="MELTVAGEAVDVTEDHVAERVERLKRMFASEPDIRVRLDTDGGK
                     VVAEVVVPIRGYRTLKSCSKADDASGAVEKAVKKLEWKYKLFWTRGSFRFGDCC"
     gene            complement(8834..9301)
                     /locus_tag="VFQ10_05130"
     CDS             complement(8834..9301)
                     /locus_tag="VFQ10_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964755.1"
                     /translation="MRILRWLKIPGMVIGFTAGLMVGALAAVANASTGYIIVAALGLG
                     IPLAIFGMIYDALLDAGKLNFGYAAPTALYGTLTFPIARLAQEVLLTQIFGQGITLQQ
                     ESGVLSFLAYQAIMGFGYGIGFLMIHGQIIELSAWRAYRKQAKEQANQPSTAE"
     gene            complement(9324..10742)
                     /locus_tag="VFQ10_05135"
     CDS             complement(9324..10742)
                     /locus_tag="VFQ10_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET9964756.1"
                     /translation="MPIRELPEAPASLHILGPGMLLAALGVGLGETYLWPRLVILFGP
                     DIRWLFFIGVTLQAVVMLEMARWATSTGESIFFGASRLWRPIMWLFFAIAILVYIWPG
                     HVALGAQAFESLTGITWYIPAVIGLILIGVIISMAKIVYNVVEMILSTLIGVLVIGSA
                     IVAALVGNLAFVWTTITGLFAFGYVPEGVLTGPRAATLFPLIVGALAFAGPSGMQQMW
                     YTLWLRDKGAGMGTYIPKIRGLTHLEEEESIPARGFMFDTEDPQNMRRWKGWRRWVHF
                     DAWVLFWGITMLTTIIYTVLAQSAVQIDPGVKDAILADDEGATLNGMSGAFASAGGAF
                     MGGVFFVFIAVIGWKMTFGIFDAFSRGQADMTYYFIRPTQRFGFSKLYFVFLWFVILF
                     GIAMLFVTGEEDGPAAILDILSVLSTFAMGAYCLLLLGTNNLLLPKKIRPNIITNILL
                     LFAAFFYLGALAYSYFAFGAII"
     gene            11391..11516
                     /locus_tag="VFQ10_05140"
     CDS             11391..11516
                     /locus_tag="VFQ10_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964757.1"
                     /translation="MHKIKKLISAMAPSAQRFRASGKGSPGRGGGPRLTDRKHYK"
     gene            11616..12326
                     /locus_tag="VFQ10_05145"
     CDS             11616..12326
                     /locus_tag="VFQ10_05145"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET9964758.1"
                     /translation="MEPTETDTPKPMIEPIRRVKLYEEVASRTRDLIVSGELRPGQAL
                     PSERKLAEQFKVGRAVIREAIRRLEVSGLVESRHGGGNYVREVTVEHLVAPIATVLND
                     IGHLREELMEARLFFEPQIAREVAKSATPEDLRQLEDVIRRQEVRTRDGQETAEEDAE
                     FHRLLARATHNTVVVRVMEVIDNLLKDEHARWFRSAERSKNSLEGNRRILEAVRRREQ
                     GAAQKAMEQHIQEIARGF"
     gene            12489..12974
                     /locus_tag="VFQ10_05150"
     CDS             12489..12974
                     /locus_tag="VFQ10_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9964759.1"
                     /translation="MYRRILAAIDGSPASADTIGQAVRLARELDADLYTISVQEWPRP
                     LEVIGMEVEIPQALHDEFFDDCQYRIRCAAEEAGVRLKSTKVTKGIPATAILRHIEET
                     GFDFVILGRRGRGFAHRLRWTSTAHKVIACANCPVIVCPTCPDETVRVSRDVPAASDV
                     V"
     gene            complement(12986..13474)
                     /locus_tag="VFQ10_05155"
     CDS             complement(12986..13474)
                     /locus_tag="VFQ10_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964760.1"
                     /translation="MNYSELTAKDLEVLREQITEYYETYYAELRSQLSGHDPATFQSV
                     PEVFKGYRRIVTELGRDGVVVTHWPSSQDSFDFHISPDRVAKDLAAEQCGGEQIIDYP
                     PGSDFGTYEIAEPLQLVVDDGVVWVAQWTRLVISSRLDAWLSTDQARTAARKDLLPHR
                     EA"
     gene            13819..14367
                     /locus_tag="VFQ10_05160"
     CDS             13819..14367
                     /locus_tag="VFQ10_05160"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9964761.1"
                     /translation="MNRESGQDPFAGLKSGGEEYSVYDAHYERIGRVDDVFLDERDAV
                     SYIGVQMGFFGTNSTLVPAEIVRVNDRRRLIEVAAEAETIKHAPHFGNEETVSPELED
                     RVRTYFGLEPLLPPSDPYPPEDTVRFGPDDRVDTEPGERMEAQNRQSGLAGEPVAGGR
                     VPEDRWDRETTADDVTIHRRRK"
     gene            14422..14961
                     /locus_tag="VFQ10_05165"
     CDS             14422..14961
                     /locus_tag="VFQ10_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964762.1"
                     /translation="MSESGSEAARRLTEAFGEPYREVVRNAVDAQQRNVQLAQGWVES
                     VTGVLESQAEANRALTRAMESHTNAVEEAIKSQERTSRALAESLEAYKDVIERNTVLQ
                     EKSTNLVQGFFGDVTSELREGMQGSQGVARSLMEGSERQIEAFQAMLGEAMNSYANLM
                     NAPFDLYRKNLEAFGKQGQ"
     gene            14972..16468
                     /locus_tag="VFQ10_05170"
     CDS             14972..16468
                     /locus_tag="VFQ10_05170"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET9964763.1"
                     /translation="MQAGEKGLEVRNLIGGRWEERDGCDTEPVYDPATGEIVAQTPLS
                     TRDDVDRAVRKAGEAFSSWAATPVVERARVLFSFKALLEEHFEELRDLVTLENGKDAA
                     DAAGEVRRGIEVVEFACGMPTLMMGETVRDVAKGIDNSSWRYPLGVVAAITPFNFPCM
                     VPLWTLPVAIGAGNAYILKPSERTPMCSVRLGELLSEAGLPDGVFSIVNGAREVVDGI
                     LEHPGIEAVSFVGSHPVAEHVYTHGCAHGKRVQALAGAKNSMLVMPDAILEKAVPNII
                     SSAYGNAGERCLAGSVLVAVGGVADDLVERLKEAAASMKVGPGAEEGSELTPVIRDSH
                     RHKVRSYIDLGEEEGAEVVLDGREAPLEDGFFMGPTLLDRVTKEMRVGREEIFGPVLS
                     VVRVDTLDEAIEFTNGSHFGNACSIYTEDGAAVRHWRERVEAGMLGVNVGVAAPMAFF
                     PFNGVKNSFYGDLHATGKDGVRFFTENKVEITRWLSDPAGDQEVVERS"
     gene            16658..17740
                     /locus_tag="VFQ10_05175"
     CDS             16658..17740
                     /locus_tag="VFQ10_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET9964764.1"
                     /translation="MRRLGLIISLAVLAALLVSMATLSAGAQTTNNTSQLSVSQGTVT
                     GANPSKAERRAAKSDMAEEERLPDYSQVVDNTNSLRFEAPGWKQGGSDVWAHGGKYVS
                     AGASSSDARFKLKTPTSGDYALYAWWPARKGNSSAARFGVETVRGTKWIEANQTSDGG
                     MWVKLGTYEMKAGDRYVVRISPGGGSGEVIADAVALVRGAASPPPKDLAPAEGSAGSG
                     KNLYGASNTAIERRDIVRYGRTHIGTPYYHSPPDACWAYEKEDCSCFTRLVYQQFGKE
                     LPDNPTEQYWHKDRKWVSRDNLQRGDLVFFKENGPDNPITHVAMYSGNGYVLHASSYY
                     AYQKVVESKMENIEGYFGARRIRHLE"
     gene            complement(17770..18711)
                     /gene="sppA"
                     /locus_tag="VFQ10_05180"
     CDS             complement(17770..18711)
                     /gene="sppA"
                     /locus_tag="VFQ10_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET9964765.1"
                     /translation="MALGLIGLAILAVVLILAAGGPTAPAVYAEEYVAGDGASRIAVV
                     PVEGTIATADNTVGGIQPTTTPEGLADALRQAGQDTSVVAVVIEVNSPGGGVTASDEM
                     HQSILDFRENTSKPVVVSMGDTAASGGYYIATAADRIVANETTLTGSLGVIIPLNNFA
                     EAAEKYGIRQVVIKSGKYKDMGSAFREMRPDEREIFQSIVNQDYAEFVEVISEGRGIP
                     EERVREIADGRVYSGSQARDLGLVDSFGGLDEASATAGRLAGARDTTVVRYVQEPTLT
                     ETLLARLAPQEPQAQQIMEATGLDLEPKPYYLYLPGA"
     gene            complement(18967..>19537)
                     /locus_tag="VFQ10_05185"
     CDS             complement(18967..>19537)
                     /locus_tag="VFQ10_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964766.1"
                     /translation="EELPPVSLYKIGDAYFVKDGNHRVSVARQQGIEMLDAEVIELRS
                     RVPVNSALTARDLLHKLEHRHLLERLPIDRVLPEIEVEFSDVADYRRLATYIEAHGFR
                     VSQLWKRYVSPEEVLRDWYEYDYCPIAEMIREEHILDSFPDRTELDLYLWIVYHRERL
                     ALEARDEKISPQAAKDDILKNVPRRRRRP"
BASE COUNT         3966 a         5886 c         6141 g         3544 t
ORIGIN      
        1 ttccttcctc tgccagctac cacctacgcc gagatgctct ccgagcggct gaggaagcac
       61 ggcacccgct gctacctgat caacaccggt tggtccggcg gtccatacgg cgtaggtagc
      121 cgtatagaca tcgccgccac ccgccagatg gtcagctccg tgatcggggg aaagctggac
      181 gccgccgaga ccagaaagga ccccttcttc ggtctcaacg tgcccgtcgc ggtgccggga
      241 gtcgatggca gcgtcctgaa cccacgcggg acctgggacg acggcgacgc ctacgacgag
      301 caggccagga aactcgccga cctcttccgc gagaacttct cgaggttcga gtcccaggtc
      361 tccgaggagg ttcgcaaggc aggaccacac ttctgactga ttgccaaccc ggcgtagaag
      421 gccgcctcca cccgtagagg cgactgccgc cccgtgctgc gacctgataa cgtttgtgcg
      481 gagtcgaaga tcgggggcca cagaatcttc tcgatcgcag ggctctgttt ggatgtgttc
      541 ttgacctggg aggagtccgc tctctgactt cgcagagagc aagtacttgg cgggctgttc
      601 acgagctcga cagggccgcg gagggtgcgg accgggctag ctcgacgcca cggtggttcg
      661 tggtatctct acatcccgct taccatccgg ggcgtgcatg tttacaatca tggcagggag
      721 tgagaagacg agtggaggag gcttgtggca tacacggacg atctccgaga gcatgagcaa
      781 gtcctgctcc gattgctgga cgagatgggg agaagtctcg ctcggcagga acggctattg
      841 gaggagatag agaaccggca cgtccagatg cgaaactcct ggatccagga gggggcggac
      901 gggccgttcg tgctgccgga tcagcaagcg cttgcagacg agttgcagcg tgaactcgag
      961 cgtgggcgcg accaggagct cgagctacat gagatcctgg acgtgttgaa gcgccggtgc
     1021 gagctggaag accggctact cgatgaactg gagtcccacc tacctcgaga acatcgggac
     1081 caattaccac tagaactggt ggactgtttc gagcggcgcg accggctcct gggagacgta
     1141 gagctcaaat cgttccggga gaagagagat cggctcgcgg agacgatgca gctcctgccg
     1201 gacctggaag ggctgctctt cgaggccgtg gaacgccatc tcgttcggct ccaccgcgag
     1261 ggtggttaga gaatcagtcg tcaagagtcc acggggcggc gaggttcgat ctgatgaaga
     1321 tctccacgcg tctttgtgag gatcgtgggc gagactttca gcgccatggc cagcttccag
     1381 atcgtgtcca ccgaggcatg aatctcctca gaggccgggt ctacgctctc tgccctggct
     1441 atcgagccgg gctccagctc cgccagctcg tcgagttgct cttgggagag ggaggcttcc
     1501 tccctcaagg cgcgtagctt ctccacggcc aggcccggct cttcgagaac cgtcggaaga
     1561 gggggagcgg ggaaggtaga caggtccgag atctcaaccc cgaagattct ggtgagcttc
     1621 tggacgatga tctcgaggac gtgcacccgg ctctccctct ccatctcctc ggcccatgtg
     1681 ccgctctggc cgaccatcaa ggagagctcc tcttgtgaga gaccggcctg ctctcttagt
     1741 tcccgcaaac gagccccgtc tactaggtat aattgcatct cggtcgttcc tttctggttc
     1801 gttgaagatc tcgcccttcg cctcccaagg ctcaatccgc tatatcgctc gggccttcga
     1861 gagtcggctc aggtgggtca gggtagcttg tcaactccga cctccccacg aagaggatct
     1921 gcatgagatc ctcgccgagg ttctccctga tctcggtccg gtctgcggtc tccaatctta
     1981 ttaccactac ggggttcatg ccgatggatt cggccaactc atcctgcgac atgaacaccc
     2041 tctcccttgc agccgccaac ttcgccccgt ctaccttcac gaccacacct cctcgctctg
     2101 tctccagcgt ctggtccatg acagccgtga ccgggagcga tcgccagata ccgcgctctt
     2161 ggaagacatc gccctcacat ccttatggtg caattctacc caaaggccgt ctcgaagtcg
     2221 tgctggaagc agagggtctc cggcctatgc tgagagtcag gatgttccga gaggagcgtg
     2281 cctggacttt agcggacctg cagttcctct cctacggagg caaagaccca actcaggccc
     2341 tgctcgccat catccggatc gacaccgacc acgtcatcgg acctgcgaag cgctcggtat
     2401 cgtgaccgag tacccctact agccgaccat tacaggacct ttatgcgcct gcgaaccctg
     2461 gtcgcggcaa gggcagcggc gatctcgtcg aaggagcgag ccggggtcga atcagcctcg
     2521 aagagggagc gtccgggctc acccgtggca tcaggcacga tcgggatgcg tcccagcacc
     2581 tcagagccta gctctcctgc aaccctatct cctccgtcac ccccaaagat ctgtaactgc
     2641 tcgccgcact cagggcactc cgcataagcc atgttctcca ccacccctgc taccttcagg
     2701 tgcgcctgca cggccatctt gccggccttg acggccaccc gggccgcatc agcctgaggg
     2761 gttgtgacca ccagcgcctc agccttgggg atcatctgcg ctacggtcag ggccacatcg
     2821 ccggtgcctg gtggcatgtc cacgatgatg aagtcgggct cgtcccagta gacgtcccgc
     2881 atgagctggg tgagtgcctt gttaactatg ggggctcgcc agatgatagc ctgcccctcg
     2941 ttcacgaagt tgcccatgga gatgaacttc aacccttgat tagactctat ggggaagatc
     3001 acccccccaa ccacgttcgg cttgtcgagc gagcccagca tcacggggat cgaagagcca
     3061 tgcacgtctg cgtcgagtat ctcaacggag tgccctgccc ggtcgagcgc cgctgcgagg
     3121 ttgacggcga cggtactctt gccgacgcct cccttgccgc tcaccactgc gataatgcgg
     3181 gttttggact cgtcgcggaa ggcgggagca agctctgagg gacccccgtg cagggaggtg
     3241 agcagattct ggcggtcttc gtcggtcatc acgccgaagt ccacatcgac ttcctcgacc
     3301 ccttcgatca tcttgagcct gtcggtgatg tccgtggtga tcctgtgctt catagggcag
     3361 cccggcgtgg ttagcgcgat ccccacggcg acatatccac cctcgatctt tacgccgcgc
     3421 accatgttca aggaaatgag gtcacgcccg atctcagggt cccttacatc cctcaaagcc
     3481 tcccgaatgc cagcctccga cagaccatcc ggaggctgat ggatctctcc tccatctcct
     3541 gagtcgggga cggcctccgg cggcgcattg ccgtcttcgg cgttggtgtg ccgccgggtc
     3601 cacttgttta ctttcatccg ttgacctccg tcgctgctct gtgttcgtcg ccaagcggcg
     3661 ttggcgtacc gccgtgtcag gcatcggttt tgagggatgt tatcacgagc gtttctatta
     3721 tcacgagcgt ttctatctag tggcggggcc gttgagcgga tttctccacc ctgctatgat
     3781 caagagagga ggaaaggaga agaacatggc caggacgatc cggtgcacgg aagcccagag
     3841 gacactggat ctcgaggtcg atcagaactg cgacttcgtt gcccgcgcgg agaccgagga
     3901 cgaggcattg gcagagttcg cggaccacat gcaaacggta cacaactgga acgagatctt
     3961 cgaaccggac ctcgagaagg tccgcgccca aaacgtccgc aacgacgtct agcaactcgc
     4021 gccgaagcgg ctcgatggat ccttccgcaa actcgccccc ggtgcccaga ttcgtgttcg
     4081 tcgcctacta ccccgagggg ccgcacgccg gtcccatcac catcggcctc ttcggcgtcc
     4141 aagacggcgc cctcctgccc atcttctcca cacgggagaa ggccatagat ttcgccgtcc
     4201 gcgtccttgg tacccctcct caaaacaacg agaactggga cggggtcgag ctccccgccg
     4261 aggaccttat acagttcctc cacaccgacg cgagcatctc ctacgtcgcc gcaaacccgc
     4321 cgccatccgg ggaggtggag ctgatcccca ccccagagtt cgtcaagcgc ctcgagcagg
     4381 aggtagggga cgccgatagt ccaagagaag acttccgccg gtccctggtt cggtccttcg
     4441 tctacggaga cgtcagctga ccactcgttc ctcgaggatg gattctggac gcatagaaaa
     4501 aatgatacaa tctttggaac atgacattga ccctgttacc tgccgctgat aggatgcttg
     4561 gcaaattggt aaagtggtca ggccagagct tcgacagccg aagctgaggg gtgtaggagt
     4621 tggctctgag ggccgattac tagggaagga gaaaggatgc cgactggcag agaagctggc
     4681 gactcgattc ccggggatca agacaccatt gagggacgca cctctactga ggtacgcgac
     4741 gaggccaaga gcgtcgagtt cacttatcga ggcatcttcc agaagacgtt agcggcgaac
     4801 ctgtgcaagc atgtagtgat ggcggcccgc aagcgggact ggttaggatc gacgatccag
     4861 cgctacggtg attcgccgga gcgcaatggc gtcccggcca agcagttcgc ggtcatcgcg
     4921 ccggatacgg agacgttgga aggccacatg gccaaatacg agcccaacga gctggacgtc
     4981 atcacggtct tggacgacac gatgtgcgac gggatcgagt cctgggcctg gtacgggcgg
     5041 cagccgatca acgtgcgtct caggcgcggc gggacgctgc tggtagtctc gaacaggacg
     5101 cccgaagagc ttctgaaggt catcccgcag aagccctggg attggacgct ggccatcctg
     5161 ccggggacgc cgagcctggg cgggctctgg gtctaccgcg acgacggcac ggacatgcgg
     5221 gtgctcggcg ccatagcgcg cacgcgctcc gacatcttcg agtacggcga gatagaggcc
     5281 gtggtgagcg agagcggact cggcgagaag tatctcaccg ctctcaagaa cggccacgac
     5341 gaggtcgaaa cccacgaggt caaggcgggc gagggcgcag aggacgacta cgaaccggtc
     5401 aagctgccgg ggtggcgcga gatgcgcgag gccatcatcg tcgaggctgt caagctgggt
     5461 gagcgcaacc cgcactacaa gacctcctct gacaggacca tgcggccggt tgtcaatttc
     5521 gacacgtgca tcaagtgccg acagtgctgg atcgactgcc ccgacgagtg cttcgatatc
     5581 tccgatgaag agctgcaccc ggtcaactac agctactgta ccggatgtgg catctgcgcc
     5641 aacgtgtgcc cggtcgagga ctgcatagta atggtcaacg acctcgaatt tgtcgaggag
     5701 ggcgacaaag atgtcttccc gatgtggaaa gaggacaaag atggctacca cgagtggcgg
     5761 gacgagaaga tagcgcgtgg gaggatagac cacgacaact tcttcaacgt cgacacctgg
     5821 gcgaagtgag aggaggggag gcgcgatgac cacccaggat acgaccgggc gtcggcaggc
     5881 acggcaggaa gaaaagctga tgacgggcac ggaggcggtt gcccaggcgg tccgcctggc
     5941 ggacgtcgag gtgacggctg cgtacccgat ccgcccctac gacggggtca tgcaggctgt
     6001 ggcgaagctg atcgccagcg gcgagctgga cgccgagttt atcgtggcgg agggcgagca
     6061 ctcccagttc gagatctgca agcacgcctc gtccgtcggc gcccgcgtgt tcgtgggctc
     6121 ctcgggcgtg ggctggatgt acgcgatgga gtccatcgcg gtcacaccgt cgctgcggct
     6181 gccggtcgta gcgatgatcg gcaaccgcgc cctcgacgat ccgggagcgt tcggggtcga
     6241 gcacaacgac gcgttgtgtg tgcgcgatct gggctggcaa ctcttatggg tcgaggacgc
     6301 gcaggagtgc ttcgacgcga cgctcatggc ctaccgcatc ggtgaggaca gcagggtctc
     6361 cttccccgta ggcctaggcg ttgacggcgc gttcatcacg cactcgcagg cgctgctcca
     6421 gatcccggac caggagagcg tcgacgcgtt cctgccgcca tatgatctgg gcgaccgggt
     6481 gctacacccg gacaacccga ttagcatagc cccccaggcc aacgaggact gggtcatgga
     6541 gatccgcaag caggccgacg aggccgccaa aagagtcaag gacgtgatct gggaggccca
     6601 caacgacttc aaggagctct tcggacgtgg cggagacaac ccattcttcg aggagtacat
     6661 gaccgaggac gccgagtacg tgctcttcgg catgggctcg ctcggcctgc cggccaaagt
     6721 catggtgcgc cggctgaggg agcagggtga gaaggtcggc ttcatccgcc tgaagtggtt
     6781 caggcccttc ccggacgagg agctggcgga ggttctgggt cgcttcaagg ccgtcggcgt
     6841 cgtggatcgc gactacgcct acggctcgcc acagcagggc ggggtcctgt tcaccgatct
     6901 gcgggcgacc ctctacgccg ccgagcggcg gcctcgcatg atcaacttca tcgggggatt
     6961 gggcggtcgc gagcttaccc ccgagatgat ggacgagatg gcggacatca ccaagcgagc
     7021 cagcgagggc gaggaagtgc ccgtcgttag ctggatcggc gtgcgcggat aaggaagagt
     7081 aaacaagtat agaaaagcaa aagaacgaag gaaggagata gaagagacgt ggttatggag
     7141 gcaccaggac gggcgctgaa gctcgatccg atcaaatcga taaagaaggc gccggcggag
     7201 gagtactacc agtccggcca tcgcacctgc cagggttgtc tgtccgccct gcccatgagg
     7261 ctcatggcga aggccgccgg tccgcgcacc attgtgaccg gcgcgacggg ctgcatgtac
     7321 gtggccaaca ccacctacat gagcaccccg tgggtcgtgc cgtggatgca cacccagctc
     7381 ggcgccgcgg ggtcgtctgc ggtaggcacg gccgccgggc tcaaggccct catgcgcaag
     7441 ggcaagatca aggacgagcc gatcaacgtg atcgccttct gcggtgacgg cggcggcgcc
     7501 gacatgggcc tggctgccat cagcgcggcg ctcactgacg tcgagtacaa cctcctgatc
     7561 ttcctctacg ataacgagag ctacgcgaat accgacatcc agacctcggg ccagacgccg
     7621 tggggcgcgg tgaccacctt cagccctcca ggcaagaagc atcgcatcat gcagcgccgg
     7681 tggaagaaga acgtccctgg catgctcgcc gcgggccacc ccgacaccaa gtacatagcc
     7741 tcgggcaccc cggccatccc tgtggacatg atggacaagg tgcgggcggc cctgagcgca
     7801 ggcgggccga cctacatgca cgtgctcgac ccctgcccga agggctggca ctacgacccc
     7861 gagcactcca acgagctcgg caaactggcc atcgagacgg gcatctatcc gctctacgag
     7921 atcaaggagg gcgtctgcaa gcttaccggc gtaaccaagc agatcgccga gggcaagcgc
     7981 aagcggaagc cagtgcggga gtacctggag acgcagggtc gcttcgccca attcaagaac
     8041 caggactacg cctacttcca gaagaaggtc gacgagatgt gggagcagtg ggttatcccc
     8101 accgtcgtcg ccttcagcta cggcggcccg atcaacatca cgggcgacat gccgaaagtc
     8161 cgcaaccgca acgtggtgcg ggagaacgca tcctgggccg gtattccggg tcacgcccac
     8221 gaggagttca aggaggctgc cgacgacccc tacgagccct ggcgcgacga caacgagctg
     8281 ggtgagtacc cgtgggcgct cccctcgacg gggcagtaag cgcgcggacg actcagacgt
     8341 agggagactg gcgggaaatc gttccccgcc agtctccttc aagcctggcc agcgtgatct
     8401 attagcagca gtcgccgaaa cggaagctgc ccctcgtcca gaacagcttg tacttccact
     8461 ctagcttctt gacagctttt tccacggcac cgctagcgtc atccgctttg ctgcacgact
     8521 ttagcgtgcg gtagccgcgg atcggaacca ccacctcggc gacgaccttc ccgccgtcgg
     8581 tgtcgaggcg caccctgatg tcgggttcgg aagcgaacat ccgcttgagg cgctccaccc
     8641 gctcggcgac atgatcctcc gtgacgtcca cagcctcgcc ggctaccgta agctccattc
     8701 gacccctatt cgcccttcac cgaatctgca ccttccgata catcatatca accggtgcgt
     8761 ccggcaacaa acccgtggcg ccggtcctgc tcaccgaccg acactccaag cgatgaggga
     8821 tcactcatgc acgctattcg gcggtactgg gttgattggc ctgttctttg gcttgttttc
     8881 tgtaggcgcg ccaggcgctc aactcgatta tctggccgtg gatcatcaag aagccgatcc
     8941 cgtagccgaa acccatgatc gcctggtacg cgaggaaaga cagcacccct gattcctgtt
     9001 gcaaggtgat cccctgcccg aagatctggg tgagcaggac ctcttgcgcc aagcgcgcta
     9061 tcgggaaggt cagggtgccg tagagtgcgg tcggggctgc gtagccgaag ttgagcttgc
     9121 cggcgtccag tagcgcgtcg tagatcatgc cgaagatcgc cagggggatg ccgagcccga
     9181 gggccgcgac gatgatatag ccggtggagg cgttggccac cgcggccagc gcccccacca
     9241 tcagtccggc ggtgaagcct atcaccatcc ccggtatctt cagccacctc aagatgcgca
     9301 ccggctacgc cttccctgcc tctctagatg atggccccga aggcgaagta actgtaggcc
     9361 aacgccccga ggtagaagaa cgccgcgaag agcagaagta tgttggtgat tatgttcggg
     9421 cgtatcttct taggcaggag caggttgttg gtccccagca gcagcaggca gtacgcgccc
     9481 atcgcgaagg tcgagagcac cgagaggatg tcgaggatgg cggcggggcc gtcctcttcg
     9541 ccggtgacga acagcatcgc gatcccgaac aggatcacga accacaggaa cacgaagtag
     9601 agcttgctga acccgaagcg ctgtgtagga cggatgaagt agtacgtcat gtccgcctgg
     9661 ccgcgggaga aggcgtcgaa gataccgaag gtcatcttcc agccgatgac ggctatgaag
     9721 acaaagaaca cgccgcccat gaacgccccg cctgctgagg cgaaggcgcc ggacatgccg
     9781 ttgagcgtgg ccccctcatc gtcggccagg atggcgtcct tcacgccggg gtcgatctgc
     9841 accgcgctct gcgctagcac cgtgtagatg atcgtggtca gcatcgttat gccccagaac
     9901 aacacccagg cgtcgaagtg cacccagcgc cgccacccct tccagcgccg catgttctgc
     9961 ggatcttccg tgtcgaacat gaagcctcgg gcgggtatag actcctcttc ctcgaggtgg
    10021 gtcaagcccc tgatcttggg gatgtaggtc cccatgccag cgcctttgtc gcgcagccag
    10081 agcgtgtacc acatctgctg catccccgaa ggaccggcga aggcgagggc ccccacgatg
    10141 agcgggaaga gcgtggcggc cctgggaccg gtcagcacgc cctccggcac gtacccgaag
    10201 gcgaacagac ctgtgatagt agtccacacg aacgccaggt tacccaccag cgccgcgacg
    10261 atcgcggagc cgatcacgag gacgccgatg agagtcgaca agatcatctc gacaacgttg
    10321 tagacgatct tcgccatgga gattatgacg ccgatgagga taagtccaat tacggccgga
    10381 atataccagg tgatccccgt caggctctcg aacgcctggg ccccgagcgc cacgtggccc
    10441 ggccagatgt agaccaatat ggctatggcg aagaacagcc acattatcgg tcgccacaga
    10501 cgggacgccc cgaagaagat gctctcgccg gtggacgtgg cccagcgggc catctccagc
    10561 atgacaaccg cctgtagggt aacgccgatg aagaagagcc agcgaatgtc cggcccgaag
    10621 aggatgacga gtcgcggcca cagatacgtc tcgcccaagc ctacccctag cgccgccagg
    10681 agcatgccgg gaccgagaat atgcaatgaa gcaggagcct ccggcagttc ccttatcggc
    10741 atcggctcct cgcgacccgc atgccataac ttttcttcct gtacttcctc ggtagccgca
    10801 ccgcccctat ttgtgtccac cacaatctcc tttcataccc cgatcggtta gtgcccaacg
    10861 cgcctgtgat cgaccgccac tgacatcgat cgaatggtca gatgcacccc gtgtcgcgtg
    10921 tgaccccgaa cagcgaccga acagcgagct tccacgaccg tctaatgcct tccccagagc
    10981 cgtgaaaacg agcgccaatg cagagcatcc ttacccgatt ttgctaaccg acctatctgc
    11041 ctttcccact tgcgacaggt cataccagca tacacagggg cctgatttgt gtcaagaaga
    11101 atattggccc cccgaagcgc ccacatgcct ccctattacc cgctaccgag atcggttgtc
    11161 ccctcgccag cactgcggtg tagggtcccg aacctaattt tgtgatacct ttctcaatga
    11221 ttggtactat ttgcttgaat cttgatacaa ggaagctaca atcaagatca agaactggac
    11281 ataccagttt ggaagacgga aatgacggat gttagcaacg ttgtgcacct agatcgcacg
    11341 ttgataacag gaatgagccg attcagagag gaagagagga gtggatcttg atgcataaga
    11401 tcaagaagtt gataagcgcc atggcaccca gcgcgcagcg ctttcgtgcg tcgggcaagg
    11461 gctcgcctgg caggggcggc ggaccgagac tcaccgaccg caagcactac aagtaagctg
    11521 acacaaccct gacggtttcg gccccctccc gcaactgcgg gagggggccg aacgcttgag
    11581 actggctcgg gtgcagagat gctaaactga atcaaatgga accgacggaa accgacacgc
    11641 ccaagcctat gatcgagccg atccgccgcg tcaaactcta tgaggaggtg gcgtcccgga
    11701 cccgcgacct catagtcagc ggcgagctcc ggccggggca ggctttgcct tcggagagga
    11761 agcttgcgga gcaattcaag gtgggccgtg cggtgatacg ggaggcgatc cgtcggcttg
    11821 aggtgtccgg cctcgtggag agccgtcacg gtggtggcaa ctacgtcagg gaggtcaccg
    11881 tcgagcatct ggtagctccc atcgccaccg tgttgaacga cattgggcac ctacgcgagg
    11941 agctcatgga ggcgaggctg ttcttcgagc cgcagattgc gcgggaggtc gccaagagcg
    12001 ccacgccgga ggatctgcgg cagttggagg acgtcatacg ccggcaggaa gtccgcacaa
    12061 gggatggtca agagacagcg gaggaggatg cggagtttca tagacttttg gcgcgcgcga
    12121 cccacaacac ggtggtggta cgggtgatgg aggtgatcga taacctgctc aaggacgagc
    12181 acgcgcggtg gttccggtcc gccgagcggt cgaagaattc gctcgagggc aaccgccgta
    12241 tcctggaggc cgtccggcgt cgcgaacaag gagctgcaca aaaggctatg gagcagcata
    12301 tccaagagat agctcgcggc ttctagtcga ttgacgcggc tgctgctcgg gtttcctcac
    12361 tgaactgcac aaaccttaga ttagttcgcg gccccacctt ttgcgcgctg tctcgccacg
    12421 ttttgctcta atgctataat gcctacatct cgaggtgcat ggggtgcgag tttcgaaggg
    12481 tgataggcat gtacagaaga atactggcgg ccattgacgg atcgccggca tccgccgaca
    12541 ccatagggca ggcggtacgg cttgctcggg agttggacgc tgatctctac accatctcgg
    12601 tacaggagtg gcccagaccg ctcgaggtca taggtatgga ggtagagata ccgcaagcgc
    12661 tgcacgatga attcttcgac gattgccagt atcgaatccg ttgcgcggcg gaggaagccg
    12721 gagtcaggtt gaagtctacc aaggtgacca aaggaattcc ggcgactgcg atattgcgcc
    12781 acatcgagga gacgggtttt gacttcgtaa ttctgggccg gcgaggccgt ggattcgcgc
    12841 accgtttacg ctggacctct acggcccaca aagtcatagc gtgcgcgaac tgtccggtga
    12901 tagtctgccc gacctgtccc gacgagactg tgagggtaag tcgagacgtg ccggcagcct
    12961 ccgacgttgt atgacgttac ctagatcaag cttctctgtg gggtagaagg tcctttctgg
    13021 cggctgtgcg ggcttgatcg gtgctcagcc aggcgtcgag cctgctggag atcactagac
    13081 gcgtccactg ggctacccag acgactccat catcgaccac gagctggagg ggttccgcga
    13141 tctcgtaagt cccgaaatcg ctccccggcg gataatctat gatctgttca ccgccgcact
    13201 gttcggctgc caggtccttt gccacccggt cgggagagat gtggaaatcg aaagaatcct
    13261 ggctgctggg ccaatgggtc accaccaccc cgtcccggcc gagttcggtg acgatccttc
    13321 tgtacccctt gaaaacctcg ggaacggact ggaaggtcgc cggatcatgg ccagatagct
    13381 ggctccgcag ttccgcgtag taggtttcgt agtactcggt tatctgttcc ctcaacactt
    13441 cgaggtcttt ggcggtcaat tcgctgtaat tcaccattcc tcctatggat caggccggcc
    13501 ctcttcggga tcgtccgacc aaacccactc tgtagccgag gacgtaggag acgaactccc
    13561 gtctggtcac gttattgcat tgaaacatag ccttcatgat gggacacagc gcacgggctg
    13621 ttagtcacgc ctggaagttg ggtattctca gagaatacgc ccaggcgacg gcgaggaggc
    13681 atcagctggt ccaaagccaa cgaagcggta ggacatgtag gagcaggatg cgtccagaac
    13741 atgagtggga agggtcgggg aggcgccgtg cctggtccct cgatgtaaag gctactattt
    13801 tttggaaagg atgaagcagt gaatagagaa agtgggcagg atcccttcgc ggggctaaag
    13861 agcggcggcg aagagtacag tgtctacgac gcccactacg agaggatcgg cagggtggac
    13921 gatgtcttcc tcgacgagcg ggacgcggtg tcttacatcg gcgtacagat gggtttcttc
    13981 gggactaact ccaccctcgt gccggccgag atcgtcaggg tcaacgacag gcggcgcctg
    14041 atcgaggtcg ccgcggaggc ggagacgatc aaacacgctc cgcacttcgg caacgaagag
    14101 accgtatcgc ccgaactcga ggacagggta aggacttact tcgggctcga acctcttctg
    14161 ccacccagtg atccttaccc tcctgaggac acagtccgat tcggtcccga cgacagggtg
    14221 gacaccgaac ctggcgagag gatggaggcc cagaaccggc agagcgggct ggcaggagag
    14281 ccggtagctg gtgggcgcgt cccagaggac cgctgggaca gggagacgac ggcggatgat
    14341 gttacgatcc accgcaggag aaagtgagag cggccggacg acgggtaaaa tgtccgggga
    14401 gagcgttcga aggaggaacg agtgtcagag agtggatccg aggcggcgcg gaggttgaca
    14461 gaggcgttcg gtgaacccta ccgggaggtg gttcgcaacg ccgtcgacgc ccaacagagg
    14521 aacgtccagc tcgcccaggg ctgggtcgag agcgtgaccg gcgtgctgga gagccaggct
    14581 gaggccaacc gggcgctgac cagggcgatg gagagccaca cgaacgccgt ggaggaggcg
    14641 atcaagagcc aggagcgcac cagccgcgcc cttgccgaga gcctcgaggc atacaaggat
    14701 gttatcgagc gtaacacggt gctccaggag aagagcacga acctcgtcca gggcttcttc
    14761 ggtgacgtta catcggagct cagggagggc atgcagggta gccagggggt ggcccgcagc
    14821 ctgatggagg gctcggagag gcagatagaa gcgttccagg cgatgcttgg cgaggcgatg
    14881 aactcctacg ctaacctgat gaacgccccc ttcgacctct accgcaagaa cctggaagcc
    14941 ttcggcaagc agggccagta aggaggacga gatgcaggcg ggtgagaaag gcctggaggt
    15001 aaggaaccta atagggggaa ggtgggagga gagagacgga tgtgacacgg aacccgtcta
    15061 cgatccggcc accggagaga tcgtcgcaca gacgcccctc tcgacgagag atgacgtcga
    15121 ccgcgccgta aggaaggcag gggaagcgtt ctcctcctgg gccgccaccc cggtcgtcga
    15181 gcgggctagg gtcctcttct ctttcaaggc gctccttgag gagcatttcg aggagttgag
    15241 ggacctggtc actctcgaga acggcaagga cgccgcagac gccgcagggg aggtcaggcg
    15301 cggcatagag gtcgtcgagt ttgcgtgtgg gatgccgacc cttatgatgg gcgagacggt
    15361 cagggacgtg gcgaagggca tagacaactc ctcctggcgc tatcccctcg gggtggttgc
    15421 ggccataacc ccattcaact tcccttgcat ggtgcctctg tggaccctgc cggtggctat
    15481 aggggccggc aacgcctaca tactcaagcc ttcggagagg acgcctatgt gctcggtgag
    15541 gctgggcgag cttctctccg aagcgggatt gcccgatggt gtcttctcca tcgtcaacgg
    15601 ggcgagggag gtcgtggatg gaatcctcga gcatccaggc atagaggccg tctcattcgt
    15661 cgggtcccat ccagtggccg agcacgtcta cacgcacggt tgtgcccacg gcaagcgggt
    15721 gcaggcgctc gcgggggcca agaactcgat gctcgtcatg ccagatgcca tactggagaa
    15781 ggccgtcccg aacataatat cctcggccta cggtaacgcc ggcgagcggt gcctggccgg
    15841 cagcgtgctg gtggccgtcg gcggggtcgc ggacgatctc gtcgaaaggc tcaaggaggc
    15901 cgcagcctcg atgaaggtcg gtcccggagc cgaagaaggc tccgagctca cccccgtcat
    15961 ccgcgactcc caccgccata aggtccgctc ctacatcgac ctcggcgagg aggagggggc
    16021 cgaggtcgta ctcgacggca gggaggcgcc tctagaggat ggtttcttta tgggtccgac
    16081 cctcctggac cgtgtgacga aggagatgcg ggtcggccgc gaggagatct tcggtcccgt
    16141 cctctccgta gttcgtgtgg acacgctaga tgaggccatc gagttcacga acggctcgca
    16201 cttcggcaac gcctgctcca tctataccga agacggtgcg gccgtaaggc actggaggga
    16261 gagggtcgag gcaggaatgc tcggggtcaa tgtcggggtc gccgccccga tggcgttctt
    16321 cccgttcaac ggggtgaaga actccttcta cggggatctg cacgccacag gcaaggacgg
    16381 ggtgcgcttc tttaccgaga acaaggtgga gataacgcgc tggctctccg accctgccgg
    16441 tgaccaagaa gtcgtcgagc gctcgtaaaa cccggcgcct gacgcggatc ttcaggtaaa
    16501 gtggggcgcc atccggcgcc ccactttcgc aactttcatt acccgcatgt aactcccggt
    16561 taaagaatct gaaaaattct ttaccaaact agtgcttttc tatcacggcg tcggtacaat
    16621 ggcgcctacg caagtacgaa cgttggaggg gggcctcatg cgtcggctcg gtttgatcat
    16681 atcgttggct gttcttgctg cactcttggt ctcaatggca acactttcgg ctggggcaca
    16741 gaccacgaat aacacatcgc agctttcggt ctcgcaaggc acggtcacgg gggctaatcc
    16801 ctccaaggcc gagcggcgcg cggccaagag cgacatggcg gaggaggagc gcctgcctga
    16861 ctactcgcag gtcgtggaca acacgaactc cttgcgtttc gaggcaccag ggtggaagca
    16921 aggtgggagt gacgtttggg cccacggagg caaatacgtc tcagcggggg cctccagcag
    16981 cgacgcacgc ttcaagctga agacccccac aagcggcgac tacgccctct acgcttggtg
    17041 gccggcccga aaaggcaaca gcagcgctgc gagattcggc gtggagaccg tcaggggcac
    17101 gaagtggata gaggcgaacc agaccagcga cgggggcatg tgggtcaaac tcggcaccta
    17161 cgagatgaag gctggagatc gctacgtcgt gcgcatctcc ccagggggcg gctctggcga
    17221 ggtcatcgcc gacgctgtcg cgctcgtcag gggagctgcc tccccgccac ccaaggacct
    17281 tgcgccagcc gaaggcagcg cgggttccgg caagaatctt tacggcgcct cgaacactgc
    17341 tattgagcgc cgggacatag tacggtacgg tagaacgcac atcggcaccc cctattacca
    17401 ctctccaccc gatgcctgct gggcttacga gaaggaagac tgctcctgct ttacaaggct
    17461 tgtctaccag caattcggca aggagctgcc agacaatccg acagaacagt attggcacaa
    17521 ggaccgcaag tgggtatcca gggacaacct ccagcgtggt gacctggtct tcttcaagga
    17581 gaacggtccg gacaacccaa ttacccacgt ggcgatgtac agcggcaacg gatacgtgct
    17641 gcatgcttcc tcttactacg cttaccagaa ggtggtcgag agcaagatgg agaacataga
    17701 gggatacttc ggcgccaggc gcatccgcca tttagagtag cggagagtta cagtgggttt
    17761 tggtaggggt caggctccgg gcaagtagag gtagtaaggc ttcggttcca gatcgagccc
    17821 tgtggcctcc atgatctgct gggcctgcgg ctcctgcggg gccaggcgcg ctagcagggt
    17881 ctcggtcagg gtcggctcct gcacgtatcg gaccacggtc gtatctctag cgccggcgag
    17941 cctgcccgcg gtggcggaag cttcgtcgag gcccccgaag gaatcaacga gtccaagatc
    18001 cctagcctgg ctgcccgagt acacgcggcc atctgcgatc tcacgcaccc tttcctccgg
    18061 gatgccgcgg ccctcggaga tcacttcgac gaactcggcg taatcctggt tcacgatgga
    18121 ctggaagatc tcacgctcgt ccggcctcat ctcgcggaag gcgctaccca tatccttgta
    18181 cttaccgctc tttatgacga cctgccttat gccgtacttt tcagcggcct ctgcgaagtt
    18241 gttgagcggg ataatgacgc ccaatgagcc cgtcagggtc gtctcgttgg cgacgatgcg
    18301 gtcggccgcg gtcgcgatgt agtagccgcc cgaggccgcc gtatctccca tggagacgac
    18361 gaccggctta ctagtgttct ccctgaaatc caggatgctc tggtgcatct cgtcgctcgc
    18421 cgtcacccca ccaccgggcg agttgacctc tatcactacg gccacgacgc tcgtgtcctg
    18481 ccccgcctgg cgtaaggcat ccgcgaggcc ttccggcgtg gtcgtcggct gtattccgcc
    18541 cacggtgtta tcggcagtgg cgatcgtacc ctcgacgggc acaacagcta tcctggacgc
    18601 tccgtcgccg gccacgtatt cctcagcgta gacggcgggg gctgtcgggc cgccagccgc
    18661 caggatcagg actaccgcca agatggccag gccgatcaga cctagtgcca tgagacctcc
    18721 gactacaaag ggccacctgc gccgcctcct gggctcctct gcaggcctct cgggttgcct
    18781 ggacggtcct tcggaaaaag tgtccatatc gctcccttcg ctgctgagtg ctctctaact
    18841 ttgcgctaag atcggcggcc cggtcgacag agcagcccga ccacgaggat tataagcctg
    18901 atgcagggcg gcgcacgagc cggacgacgc ggcgtctctc cgtcgtcccc tgtggcaagt
    18961 cccggttcag ggccggcgtc gtcggcgggg gacgttcttc aagatgtcgt ccttggcggc
    19021 ctgaggtgag atcttctcgt cgcgcgcctc cagcgccagg cgctcgcggt ggtagacgat
    19081 ccagaggtac aggtcgagct cggtgcggtc ggggaatgag tcgaggatat gttcctccct
    19141 gatcatctct gcgatcgggc agtaatcgta ttcgtaccag tccctaagga cctcttccgg
    19201 tgagacgtag cgcttccaga gctgggagac cctgaagccg tgcgcctcga tgtaggttgc
    19261 gagcctcctg tagtcagcga catcggagaa ctcgacctct atctcgggta gcacacggtc
    19321 gatgggtagc cgctccagaa ggtggcggtg ctcgagcttg tggagcaggt cgcgggccgt
    19381 cagcgccgag ttcacaggca ccctgctgcg gagctctatg acctcggcgt cgagcatctc
    19441 gatgccctgt tggcgggcta ccgagaccct gtggttgccg tccttgacga agtaggcgtc
    19501 cccgatcttg tacaggctga cggggggcaa ctcttct
//
LOCUS       DASTKM010000119         3851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_733166, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000119 DASTKM010000000
VERSION     DASTKM010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733166"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFQ10_05190"
     CDS             complement(<1..347)
                     /locus_tag="VFQ10_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9964767.1"
                     /translation="MRSKLSVISQSHAGTHGVMVFDPYSGKTVSLNADRPFVAASLSK
                     LYALLTLYRAASRGELSLDDKITMRSSDVWAYGTGALYKHPVGYTMTLRECAKFMIKE
                     SDNTAELMLNRYL"
     gene            complement(692..1519)
                     /locus_tag="VFQ10_05195"
     CDS             complement(692..1519)
                     /locus_tag="VFQ10_05195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9964768.1"
                     /translation="MKFGISTFVTDEGIAPGALARAIEERGFDSLFIAEHTHIPLSRK
                     SPWPRGGELPRRYYRTLDPFITLTAAAAATERLLLGTGVALIVERDPIITSKEVASLD
                     LVSGGRVIFGVGVGWNREEMENHGTDPRTRGKLTDERIWAMIEMWTKDEPEFHGRYVD
                     FDPIGMWPKPVQKPYPPIYVGGGRRAFERIAKFGDAWLANGLPPNKLEPMMGELRDVA
                     GRDVPVTVFNASSDHEDLEAYRRLDVERVLLGLPTLPESETLKQLDNLADPTWGFRP"
     gene            1663..2397
                     /locus_tag="VFQ10_05200"
     CDS             1663..2397
                     /locus_tag="VFQ10_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964769.1"
                     /translation="MLGVELLFAMELAVLYAAGSYVMGRLMRISRVGSSGSVMSRVGF
                     YLLVFPGVVLHEGAHYLACVLTRTKVTRFAPFSPGRRSNDGRLVLGYVRHERRAFPIG
                     AIIGLAPILVNPLGLLLVTALLTPLTFQEVAKPSAGAVVEGILASGFLSDTPLLAAIW
                     AYLSLSFALGSVPSREDLGSLPLLLVVFGGVLLLINLLGTGSESVLSPAIYDLSALAA
                     GLYALPAIVAVAAALTMALAGRAIRY"
     gene            2522..3700
                     /locus_tag="VFQ10_05205"
     CDS             2522..3700
                     /locus_tag="VFQ10_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9964770.1"
                     /translation="MCAPREASGGPRLLQFAAFFSSFDRFAVAPMLVTIAASLQVSLT
                     EVAATASLYYLFYGLMQPVWGMVSDRLGRVRVMRMSLFGVAVAGVLSALAPNLAVLIA
                     ARAFAGGLFAAVIPASLVYIGDTVGMGSRQKALADLMAASAVGTALATVFGGVAAYLD
                     AWRIAFAAPALAGAALAFLLVRLPEPKAFSEQKREGPLVQVGRVLAQPWALVVVGTAL
                     VEGAVILGFLTFLAPSLESVGFSPAVAGLAVGLYGLAVLGWTRAVKLLAHRLGASVLI
                     LIGASMLMLGYASGAVGPSLASVSAAAVLVGGGFAFMHSTLQTWATEVVPEARATVIS
                     LFAAALFAGSAVATMAAAPLAEAGSFHVLFALGALTAVPLGLFAGLARHRYGSNRRQS
                     "
BASE COUNT          634 a         1148 c         1207 g          862 t
ORIGIN      
        1 tccagatagc ggttgagcat cagctctgcg gtattgtcgc tctccttgat catgaacttt
       61 gcgcactcgc gcagggtcat ggtgtagccg acaggatgtt tgtagagcgc gccggtgccg
      121 taggcccaga catccgagga gcgcatagtg atcttgtcgt ccaggctcaa ctcacctcgg
      181 gaagcggctc tgtagagcgt aagcagagcg tagagcttgc taaggcttgc agccacgaag
      241 ggccgatcag cgttgagtga taccgtcttg ccagaatagg gatcgaagac catcacccca
      301 tgtgtccctg cgtgggactg ggagatgact gagagcttgg agcgcaactc ctctacggct
      361 tgggctcgtt cgggtgctgg aggttgcggt ggtttcgcag aggttgggcg atccacggac
      421 gcaatcgctg gaggcatagc cgatcgctca gagtcccata gaaagtaatc ggctaccagg
      481 aggcacaaca gaaggccgaa tatcagcagc ggccaacctc ggcgacgtcg tcgccggcgc
      541 ctttggttcc tcgcgtcccg acgcgttcga tccctcacgg tacccttgta agatctcctc
      601 ggtcgtcggg ccatggctag tgcgcacttt agcaggaacg attagcctga gtacttgcga
      661 gaattacatg atccacccgt tcttcggtca attagggacg gaagccccat gttgggtcag
      721 cgagattgtc gagttgcttc aaggtttcgc tttcgggcag cgtcggcaga cctagcaaga
      781 ctcgctccac gtccagccgc ctgtatgcct ccagatcttc gtgatcgcta gatgcgttga
      841 acactgtcac cggcacgtcc ctccccgcaa catcccgcag ttcgcccatc atcggttcga
      901 gcttgtttgg cggaagcccg ttcgctagcc aggcgtcgcc gaacttggcg atcctttcga
      961 aggccctcct cccgcctccg acgtagatcg gcgggtatgg cttctgcacc ggtttgggcc
     1021 acatgccgat cgggtcgaag tccacgtacc ggccatggaa ttccggctca tctttagtcc
     1081 acatctctat catcgcccat atgcgctcgt ccgtcagctt gccgcgggtg cgggggtcgg
     1141 tgccgtggtt ctccatctcc tcgcggttcc agcccacgcc gacgccgaag attactcgtc
     1201 ccccggatac gaggtccagg ctagcaactt ccttggacgt gatgatcggg tctcgctcga
     1261 ctattagggc cacccccgtg ccgagcagca ggcgttcggt tgcagcggca gcggcggtga
     1321 gcgtgatgaa ggggtcaagg gtccggtagt acctgcgtgg cagctcgccg ccgcggggcc
     1381 agggggactt tcggctgagg gggatgtggg tgtgttcggc gatgaacaga gagtcgaagc
     1441 cacgctcttc gatcgctctg gccagggctc ctggcgcgat tccttcgtcc gttacgaacg
     1501 ttgaaatacc gaatttcatc tcctcattga accataccgg gcaggtatct tacctgcacg
     1561 cttacctaag cgacgttcag acctccgagg tacaaggtca tagaatctga gccatgaaga
     1621 tcttcaaagg tagttgaccg gagcgtgata gacggcggag caatgctggg agtagagctc
     1681 ctctttgcta tggaactggc cgtcctgtac gctgccggct cgtacgtgat gggtcgcctg
     1741 atgaggattt cacgggtcgg tagtagcggg agcgtaatga gccgagtggg cttctacctc
     1801 ctggtatttc ctggtgtggt tcttcacgag ggcgctcact atctggcctg tgtgctcact
     1861 agaaccaagg tcacccgctt cgcaccattc tcgccgggca ggaggtcgaa cgatggtcgt
     1921 ctggtgcttg gttacgtccg acatgagcgc cgcgccttcc ccattggagc gattatcggg
     1981 ttggcgccga tactcgtgaa tcctttggga ctcctgctcg tcaccgcatt gctcacgccc
     2041 ctcacatttc aagaggtcgc caaaccctct gccggggctg tggtagaggg gatcctcgcg
     2101 agtgggtttc tatccgatac tccactgcta gctgcaatct gggcgtacct gtccctaagc
     2161 ttcgcgttgg gaagcgtacc cagcagagaa gacctaggga gtctgccgct gctgctagta
     2221 gttttcggag gcgttcttct cttgatcaac ctcttgggaa caggttctga gagtgttctc
     2281 tccccagcga tctacgatct ctccgcgcta gcggctggac tctacgctct gccggccata
     2341 gttgccgttg ccgctgccct cacgatggcg cttgcaggac gggcgattcg gtactgaagc
     2401 cgtatggggg tcgcccatcg gtgcttcatt gcctcggagt gtgggtaaga tgcagaagta
     2461 tggcctggta cgagaatgag gcggcagccc gctcccgaga catgaaacac ggagcgcgat
     2521 agtgtgtgcg ccgagagagg cttccggagg tccacggctc ctgcagttcg cggctttctt
     2581 tagcagcttc gaccgcttcg cggtcgcccc aatgctcgtg accatcgccg cctcccttca
     2641 ggtgtcgctt accgaagttg cggcgacggc cagcctgtac tatctgttct atggcttgat
     2701 gcagcccgta tggggtatgg tctcggaccg gctcgggcgc gtgcgtgtga tgcgcatgtc
     2761 cctcttcggg gtcgcggttg cgggggttct ctcagcattg gccccgaacc tggctgtgct
     2821 gatcgccgca cgagcgttcg ccggtggact cttcgctgcg gtgataccgg cctccctggt
     2881 ctatatcggc gacacggtcg gtatgggatc tcggcagaag gcgctcgccg acctgatggc
     2941 cgcctcggcc gtgggcacgg ccttggcaac cgtcttcggg ggagttgctg catacctcga
     3001 cgcctggagg atcgccttcg cggctcctgc cctcgcaggt gctgcgcttg ccttcctgct
     3061 cgtccgattg ccggagccca aggccttctc cgaacagaag cgagaggggc cactggttca
     3121 ggttggccgt gtcctcgctc agccttgggc cctggtggtg gtaggaactg cgttggtaga
     3181 aggggcggtg atacttggct tcttgacatt cctcgctcct tctctcgaga gtgtcggctt
     3241 cagccccgcg gtggccgggc tggcggtcgg actctacggt cttgcggtgc tcggctggac
     3301 cagggccgtc aagctcctcg ctcatcgtct cggggcgagc gtgctgatcc tgatcggtgc
     3361 cagcatgctc atgctgggtt acgcctcggg cgccgtagga ccttcgctcg cgagcgtgag
     3421 cgcggcggcg gtactcgtcg gtggggggtt cgccttcatg cactctactc tccagacctg
     3481 ggccaccgag gtcgtgccgg aggcgcgtgc caccgtgatc tcgctcttcg ccgctgcact
     3541 ctttgccggg agcgcggtag ctaccatggc cgcggcgccg cttgcggaag cgggttcctt
     3601 ccatgtgctc ttcgcgctgg gcgccctgac ggccgttcct ctgggactct tcgcgggcct
     3661 ggcgcgccac agatatggca gtaatcggcg gcagagctag catgatctga cgagcacaaa
     3721 gcagcgacgt ctccatggat tcctgacttg atttgaacct aacccggacg caactctgtg
     3781 caatgggggg caatgcaggg aacagaaaac ccttgcctat gcgggaattg caaacccgtg
     3841 caacgctcgg c
//
LOCUS       DASTKM010000120         7895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1437854, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000120 DASTKM010000000
VERSION     DASTKM010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437854"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VFQ10_05210"
     CDS             complement(<1..1123)
                     /locus_tag="VFQ10_05210"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HET9964771.1"
                     /translation="MRTHQRNRWGFWQSLLGRDGENKSPQKIPHHHRDHDDDEEPLSE
                     ATLQEEHHHQEQKQAPQLQIAPNDPIVAYLASASGVVEIDKLDLHSPALDAMKASQIK
                     VVVPLVSQGELIGLLNLGPRLSQQEYSADDRKLLNDLATQTAPAVQVAQLVRQQQRQA
                     QERERIEQELRVARLIQQTLLPKEVPDLEGYKLAAYYQPAREVGGDFYDFLKLDDEHL
                     GLVVGDVTDKGVPAAIVMATTRTMLRASAQRLDSPGEVLKRVNDVIVRDIPPNMFITC
                     LYAILDLESGVLRYANAGHDLPYRRRRRSSSNNGGGAEELRATGMPLGLLPGMSYEEK
                     EIVLDRGDSVLFYSDGLVEAHDPHRQMFGFPRLQGLVGAH"
     gene            1481..2323
                     /locus_tag="VFQ10_05215"
     CDS             1481..2323
                     /locus_tag="VFQ10_05215"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HET9964772.1"
                     /translation="MREIAVSSVIEAVRDLCIEANTRLPQEHLAALRRALEAEESELG
                     REVIERLLENAEVARDRCIAFCQDTGYAVFFVELGSEVRISGGDIGAAIDQGVRRGYE
                     EGYLRKSIVRSPIDRTNTGDNTPAVVYYEPAPGDGLKLTLLVKGAGCDNMSSLKMLTP
                     AEGLPAMKGFVVETIERAGPNASPPLTVGIGMGGPFEKAAVLAKKALTRPSGEPNPDP
                     DLADLERELVDEINATGIGPAGYGGTQTALAVHIESFPTHIAAFPVAVNLDCHSHRTA
                     SAEL"
     gene            2320..2877
                     /locus_tag="VFQ10_05220"
     CDS             2320..2877
                     /locus_tag="VFQ10_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HET9964773.1"
                     /translation="MSPAPIRLEAPLSKEELEPLRTGDVVRLNGVIYIARDAAHARMK
                     EAIEAGKPLPLDPEGNIVYYSGPAPARPGYALGPAGPTTASRMDPYAPLLIERGLRGM
                     IGKGVRSEAVIESMREHGSVYFGAVEGTAALLADCVKEAEVIAYEDLGAEAIHRLFVE
                     EFPVVVVNDLYGGDLYREARERWRR"
     gene            complement(2941..3633)
                     /locus_tag="VFQ10_05225"
     CDS             complement(2941..3633)
                     /locus_tag="VFQ10_05225"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET9964774.1"
                     /translation="MIRGRTLGLREGIESSARETDAAASEFEIDEPFTSLLSTTRAVL
                     FSPQRFFDELPPDGPLGAPVLYFLICSAITAVISVVASLIFLAVPVGIAVATGSLDAG
                     LLIRVLTIFVLASLVVLPALFIAGFFASVLVLHAFVRLIAGRDQRGLPATLRVSCYAV
                     GAPVAVAWIPLAGILAVFYCFYLHTIGLKRVHRISTARSLGSTLILATLLLVLAAVVA
                     VYDYNAIREVMK"
     gene            complement(3716..4132)
                     /gene="msrB"
                     /locus_tag="VFQ10_05230"
     CDS             complement(3716..4132)
                     /gene="msrB"
                     /locus_tag="VFQ10_05230"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET9964775.1"
                     /translation="MTDTPRKIQKTEEEWQDQLTPEQYRITRMKGTERPFSGEYYNNK
                     EEGVYRCVACGTPLFASATKYDSGTGWPSFWEPLDEANVETEEDRSLFMRRTEVHCAV
                     CGAHQGHVFEDGPAPTGQRYCINSAALRFDPEAKPE"
     gene            complement(4241..4654)
                     /locus_tag="VFQ10_05235"
     CDS             complement(4241..4654)
                     /locus_tag="VFQ10_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964776.1"
                     /translation="MPDLTVSAAVESGPRAYAMAGVGPEDVDVAMLYDAFTKNTVLFL
                     EDLGFCEKEEGGAFVSGGRISPGGELAVNTSGGGLSYNHPGMYGLLLIVEAVRQLRGE
                     SGDRQVENPNIALVHGNGGVLSSQVTALLGSERAL"
     gene            complement(4682..5149)
                     /locus_tag="VFQ10_05240"
     CDS             complement(4682..5149)
                     /locus_tag="VFQ10_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964777.1"
                     /translation="MGGSSFVSHLLHAAAAIDAGLCEVALITYGSTQRSGGGRLVTGS
                     ETDPYEAPYNPRYPVSMYALAASRHMHEYGTTREQLAEVAVAAREWAKLNPKAFMRED
                     LTVEDVLSSRMVSSPLSVRDCCLVTDGGGAAIVTCAERARDLEVTPVYLLGAG"
     gene            complement(5165..5389)
                     /locus_tag="VFQ10_05245"
     CDS             complement(5165..5389)
                     /locus_tag="VFQ10_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964778.1"
                     /translation="MSLRGKVAVVGVAEADLGEVGPGFTPLDLIGQATLRALEDSGLD
                     KSDVDGLFSASAYYHMPTLSVGEYLGIRPR"
     gene            complement(5386..5781)
                     /locus_tag="VFQ10_05250"
     CDS             complement(5386..5781)
                     /locus_tag="VFQ10_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HET9964779.1"
                     /translation="MNSGDSEPPAEVYRRYLESGGLGYQRCAGCGAGCGAAVFYPRVL
                     CPVCGESGLTWEISSGLGVVYATTAVYRREGDPYNVVLVDLEEGFRMMSRVEGVSAER
                     VEVGASVMLRVDREDDDPSPLFVLRGEGP"
     gene            5903..6415
                     /locus_tag="VFQ10_05255"
     CDS             5903..6415
                     /locus_tag="VFQ10_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET9964780.1"
                     /translation="MAVREGWRGRFFEDFEVGDVYEHPLGRTVTTTDNMWFTLLTQNT
                     APIHVDHAYAEKTEFGKPLVDSTFTLALVTGQSVSDVSQNVFANLGWDEVRLPAPVFE
                     GDTIYSRSEVLEKRESRSRPDVGIVKVKTTGYNQDGKTVLTFMRTVMVYKRDHAPKVR
                     RTRDADGQGT"
     gene            6412..7551
                     /locus_tag="VFQ10_05260"
     CDS             6412..7551
                     /locus_tag="VFQ10_05260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9964781.1"
                     /translation="MTYEDLRRGVREVCSRFPGEYWRGLDGGRRYPEEFVAAMTEAGY
                     LGALIPEEYGGMGLDLSAGCAILGEVNRSGANAGPAHAQMYTMGTLLRHGSEEQKTEY
                     LPKIARGDLRLQAFAVTEPEAGTDTTSIKSMATREGDHYVVNGRKIYISRVEQSDLMI
                     LLARTTPVEEVEKRSGGLSVFLVDLREAEGLTVRPRRVMMNNATSELVFEGLRLSAEN
                     IIGEEGEGFRYIIDGMNAERILIAAECVGDGRWFIEKATERAGEREVFGRRIGENQGV
                     QFPIARAYANVEAASLMVEKAASLFDRGEPCGAEANLAKLLAADASWEAANATLQTFG
                     GYGFDAEYDVERKFRETRLYQVAPISTNLILSYSAEHVLGLPRSF"
     gene            complement(7692..>7895)
                     /locus_tag="VFQ10_05265"
     CDS             complement(7692..>7895)
                     /locus_tag="VFQ10_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HET9964782.1"
                     /translation="EENSVESIAFPAISTGIFGYPVEEAAGVALGTMREEANGLEHVR
                     LIRFVLHSASDLQAHERILSELE"
BASE COUNT         1563 a         2447 c         2422 g         1463 t
ORIGIN      
        1 ggtgcgcccc cacaagcccc tgcaagcgag ggaagccaaa catctgcctg tgggggtcat
       61 gtgcctccac caatccatcg ctgtagaaca ataccgaatc tcccctatcc agtactatct
      121 ccttctcctc gtaactcatc cctggcaata gccccaaggg catccccgtt gcccttagct
      181 cctctgcccc acctccatta ttggaactgc tcctcctcct cctcctgtag ggaaggtcgt
      241 ggcctgcgtt ggcgtagcgt agtactccac tctctagatc aaggatggca tacaagcacg
      301 tgatgaacat gttgggtggg atgtcgcgca ctatcacgtc gtttacccgc tttaggacct
      361 ctcctgggga gtcaagacgc tgggctgatg ccctgagcat ggtacgggtt gtggccatca
      421 ctatggctgc tggtactccc ttgtctgtaa cgtcacctac caccagccca aggtgctcat
      481 catcgagttt gaggaagtcg tagaagtccc ctcctacctc ccttgctggc tggtagtaag
      541 cagcgagctt gtagccttca agatcaggca cttccttggg aaggagggtc tgttggatga
      601 gccgagctac cctaagctcc tgctcgatgc gctcacgctc ctgggcttgt cgttgctgct
      661 ggcgcaccag ttgggctacc tgcacagcag gtgctgtctg ggtcgccagg tcgttgagga
      721 gcttgcgatc atctgctgag tactcctgct gggagaggcg aggtccaagg ttgagaagcc
      781 cgatcaactc cccctggcta accagaggca caaccacctt gatctgggaa gccttcatgg
      841 catcgagagc aggcgaatgg aggtctagct tgtcgatctc aacaacccca gaagcgctgg
      901 ccaagtaagc cacgatggga tcgtttggtg ctatctgaag ctggggagcc tgcttctgct
      961 cctggtggtg gtgctcctct tggagggtag cctcagagag gggctcttca tcatcatcat
     1021 gatcacgatg atgatgtggg atcttttggg ggctcttatt ttcaccatcg cgccccaaca
     1081 ggctctgcca gaaaccccat ctgtttctct gatgtgtcct cacaagtgac ctcctctagg
     1141 ctgattgtag atgccaagcg tctgccagac gttacgaaaa gcgttacgaa atgccctcta
     1201 acagaaatct gccaagtaat tgtctcacgt ctcccctctt gcgtcgcctc cctgtgggcg
     1261 cagcccactc gggctgtttg gtcctccaga tgaaatgtct ctgccaggca tttccgtctc
     1321 cagtgtcgca aggcagactg cactctacct gacgaacgcc tcgcgttcgt tacgcaaagc
     1381 gttacgagag tctctcttcg aaagacctcc accgaaagag gcaggaagcg ggacgcctcc
     1441 gatgctaatc tttgcccgat gaggtcgagg tgctaagccg ttgcgcgaaa tagcggtcag
     1501 ctcggtaatc gaagcggtgc gcgatctgtg catcgaggcc aacacgcgat tgccgcagga
     1561 gcaccttgcc gccctgagac gcgccctcga agccgaggag tccgaactcg ggcgcgaggt
     1621 gatcgaacgc ctcctggaga acgccgaggt ggcccgcgat cggtgcatcg ccttctgcca
     1681 ggacacggga tatgctgtgt tcttcgtcga gctggggagc gaagtcagga tctcgggcgg
     1741 agacattgga gccgccatag accaaggcgt ccgccgaggc tatgaggagg gctacctcag
     1801 aaaatctatc gtcaggagcc ccatagacag gacgaacacg ggcgacaaca ccccggccgt
     1861 cgtctactac gaaccggccc ctggagacgg cctgaagctc acgctgctcg tcaagggcgc
     1921 gggctgcgac aacatgagct ccctcaagat gctcaccccc gcggagggcc tccctgcgat
     1981 gaaggggttc gtcgtcgaga ccatcgagag ggctggccca aacgccagcc cgccgctcac
     2041 cgtcgggatc ggtatgggcg gccctttcga gaaggcggca gtactcgcca agaaagcgct
     2101 tacccgtcct tccggcgagc ccaaccccga tcccgatctg gccgatctcg aacgcgagct
     2161 agtcgacgag atcaacgcca caggcatagg acctgccggc tacggcggca cgcagacggc
     2221 gctcgcggtg cacatcgaga gctttccgac ccacatcgca gccttccccg tcgccgtcaa
     2281 cctcgactgc cactcccacc gcacggcctc ggccgaactg tgagtcccgc gcccatcagg
     2341 ctggaagcgc cgctctccaa agaggagcta gagcctctga ggacaggcga cgtcgtccgc
     2401 ctcaacgggg tgatctacat agcccgcgac gcggcccatg ccaggatgaa agaggcgata
     2461 gaggcaggaa aaccgctacc cctggatccg gaaggaaaca tcgtatacta ctcgggccca
     2521 gccccggcgc gacccggtta cgccttggga cccgcagggc caacgaccgc gtcgaggatg
     2581 gacccttacg caccactcct gatcgaacgc ggtttgaggg gaatgatagg caagggagtg
     2641 cgctccgagg cggtgatcga gtcgatgagg gagcacggta gcgtgtactt cggggccgtc
     2701 gagggtacgg cggcgcttct ggcggactgc gtgaaggagg ccgaggtcat cgcttacgaa
     2761 gacctcgggg ctgaggcgat ccaccgcctc ttcgtagaag agtttccggt agtcgtcgtg
     2821 aacgacctct acggtggcga cctctaccgc gaggccaggg agcgctggcg tcgctgaaag
     2881 cagtgtccgg aaggcttccg gacactgcgc ttgtcagctt ttcagttgct gggagaccgg
     2941 ctatttcatt acctcacgaa tcgcattgta gtcgtagacg gccactactg ccgccagcac
     3001 gagcaggagt gttgcgagga tcagggtgga tccgagagat ctggcggtgg aaatcctgtg
     3061 cacgcgcttc agaccgatgg tatgcagata gaaacagtag aaaacggcca ggatgccggc
     3121 caggggtatc caggctaccg ccactggggc ccctactgcg tagcaagata cgcgcaaggt
     3181 ggcgggcaga cctctctgat cacgtcctgc gatgaggcgg acaaaggcgt gcaggacgag
     3241 caccgatgcg aagaagccgg cgatgaacaa tgcaggcaag accaccaacg atgcgagtac
     3301 gaatatcgtc aggacgcgaa tgagcagtcc tgcgtccaat gagccggttg ccacggcgat
     3361 ccccacaggc acggcgagga agatgaggct ggcaaccaca ctgataaccg ccgtgatcgc
     3421 cgagcagatc aggaagtaga gtacgggcgc tccgagtggc ccatccggcg gcaactcgtc
     3481 gaagaagcgc tgcggtgaga agagcacggc ccgcgtagtc gagagcaggc tcgtgaaggg
     3541 ctcgtcgatc tcgaattcgc ttgcggccgc atccgtttcg cgggccgagc tctctatccc
     3601 ctctcgcaac cccagagtcc tgcctctgat catcgagcct accgctcgga agtatacgac
     3661 gaggccaggg tgttagccca agcgccgatc ctcttctcga cgtacagctt cggagttact
     3721 ctggtttggc ttctgggtcg aagcggagtg cggcggagtt gatgcagtag cgctggcccg
     3781 taggggccgg tccgtcctcg aagacgtgtc cctggtgggc gccgcagacg gcgcagtgta
     3841 cctcagtgcg gcgcatgaag aggctccgat cctcttccgt ctcgacgttt gcttcgtcga
     3901 gtggctccca gaagctcggc cagcccgtac cggaatcgta cttggtggcc gacgcgaaga
     3961 ggggcgttcc gcaggccaca cagcggtaga ctccttcctc tttgttgttg tagtactcac
     4021 cactgaaggg ccgctctgtg cccttcatcc gcgttatgcg gtactgctcg ggggtgagct
     4081 ggtcctgcca ctcttcttcc gtcttctgta tcttccttgg cgtatcagtc atgtggagag
     4141 tctaccacgc cggctcccag ctcttgagga ggcgaacaac gaacgtcccc tgcttaatct
     4201 tctcttgagt ttgtcgactt catgccttga gtagcaccgt tcagagcgct ctctcggagc
     4261 cgaggagggc ggttacctgg ctggagagga cgcccccgtt tccgtggacg agggcgatat
     4321 ttgggttctc cacctggcgg tcgccacttt ccccgcggag ctgacgcacg gcctcgacga
     4381 tcaggagcag cccgtacatg ccagggtgat tgtatgagag accacccccg ctggtgttga
     4441 cggcgagctc tccccccggt gagatcctgc cgcccgagac gaaggcccca ccctcctctt
     4501 tctcgcagaa tccaagatcc tccaggaaca acacggtgtt tttggtgaag gcgtcgtaga
     4561 gcatcgccac gtcgacgtct tccggtccta cgccggccat cgcgtaggcc cgcggccccg
     4621 actctacggc ggccgagacc gtgaggtcgg gcatctggct tatgctacgg tgccagtggg
     4681 cctaaccggc cccaagcaga tagacgggtg tcacttcgag gtccctggcc ctctcggcgc
     4741 aggttacgat tgccgccccg cccccgtcgg tgacgagaca gcaatccctg acgctgagag
     4801 gcgaggagac catgcgcgaa gagagtacgt cctccacggt taggtcctcg cgcatgaatg
     4861 ctttcgggtt gagcttcgcc cactctctgg cggcgacggc gacctcggca agttgctcgc
     4921 gcgtggtgcc atactcgtgc atgtgccggg atgcggcaag ggcgtacatg ctgacggggt
     4981 agcggggatt gtaaggggcc tcgtaggggt cggtctccga gcctgtgacg aggcgtcccc
     5041 cgcccgagcg ctgcgtcgag ccgtaggtta tgagggcgac ctcgcagagc cccgcgtcta
     5101 tcgcggcggc ggcgtgcaag aggtgggaga cgaatgagga gccacccatg ctcgtcgcgt
     5161 ccgactaacg gggtcttata ccgaggtact cgccgaccga gagggtcggc atatgatagt
     5221 aggcagaggc cgagaacagg ccgtccacat cagacttgtc caggccggag tcctccaggg
     5281 cgcggagggt agcctgcccg atgagatcca ggggcgtgaa ccccggcccg acctcgccga
     5341 ggtcagcctc agcgaccccg acgacggcca ccttaccccg caggctcacg gaccctcgcc
     5401 tcttaacacg aagagcgggg aagggtcatc atcttcgcgg tctaccctga gcatcacgct
     5461 cgccccgacc tctaccctct ccgcagacac accctcgacg cggctcatca tcctgaagcc
     5521 ctcttccaga tcgacgagga cgacgttata ggggtcgccc tcccgcctgt acacagccgt
     5581 cgtcgcgtag accaccccca ggcctgagct gatctcccag gtgagaccag attccccgca
     5641 gacggggcag agaacgcgcg ggtagaagac cgcggcaccg caaccggcac cgcaaccggc
     5701 gcagcgctgg tagccgagcc cgcccgactc caggtagcgc ctgtagacct cggcgggtgg
     5761 ttcgctatct cccgagttca tcacatacct cctgtggttc accagcactg aggtaagcat
     5821 caaccaagca gccaataggc gcaacccagg ctccgaacgg tggatgctat attgtgaatc
     5881 ccagcgcaag agagggagtg gtatggccgt aagagagggc tggcgaggac gcttcttcga
     5941 agatttcgag gtcggtgacg tatacgagca tcctcttggt cgcaccgtga cgaccaccga
     6001 caacatgtgg tttacgctcc tcacccagaa taccgccccg atacatgtgg atcacgccta
     6061 cgcagagaag acggagttcg ggaaacccct cgtcgactcc accttcacgc tcgccctggt
     6121 aacaggacag agcgttagcg acgtctctca gaacgtgttc gccaatttgg ggtgggacga
     6181 agtgaggctt cccgcccccg tcttcgaggg ggacaccatc tactctagga gcgaggttct
     6241 cgagaaacgc gagtcgcgct cacgccccga cgtcggcata gttaaggtga agaccacggg
     6301 ctacaaccag gacggtaaga cggtgctaac gttcatgcga accgtgatgg tgtacaagag
     6361 agatcatgcg ccgaaggtcc gtcgcaccag ggatgcagat ggtcagggaa catgacctac
     6421 gaggatctac gccggggcgt cagggaggtg tgctcccgct ttcccggtga gtactggcgc
     6481 ggtctcgacg gcgggcgccg ctacccggag gagttcgtcg cggcgatgac cgaggccggt
     6541 tacctcggcg ctctcatacc ggaggagtac ggcggtatgg gcctcgatct ctcggcagga
     6601 tgtgcgatcc tcggtgaggt caaccggagc ggggccaatg cgggccctgc acacgcccag
     6661 atgtacacca tgggcacgct cctcaggcat ggctccgaag agcaaaaaac agagtacttg
     6721 ccgaagatag ccagaggaga tctgaggttg caggccttcg ccgtcacaga gccagaagct
     6781 ggaaccgaca cgacctccat aaagtcaatg gcgacgcgcg agggcgacca ttacgtcgtg
     6841 aacgggcgca agatctacat ctccagggtc gagcagtctg acctcatgat cctgctcgcc
     6901 cgcacaacac ccgtcgagga ggtcgaaaaa cgctccggcg gcctctccgt cttcctcgtg
     6961 gacctaaggg aggcagaagg cctcaccgtg agaccgaggc gcgtgatgat gaacaacgcg
     7021 accagcgagc ttgtcttcga aggcttacgg ctttctgcgg agaacattat cggtgaagag
     7081 ggagaaggtt tccgctacat catcgacggc atgaacgctg agcgtatcct catcgccgct
     7141 gagtgcgtgg gcgacgggag gtggttcata gagaaggcca cagaacgcgc cggagagcgc
     7201 gaggtgttcg gacgtaggat cggcgagaac cagggcgtcc agttccccat agcaagggcc
     7261 tacgcgaacg tcgaagccgc ctcgctgatg gtggagaagg cagcctccct cttcgaccgg
     7321 ggagagccgt gcggtgccga ggcgaacctg gcgaagctcc tggccgccga cgcctcgtgg
     7381 gaggcggcca acgccaccct tcagaccttc ggcggctacg gcttcgacgc cgagtacgat
     7441 gtagagcgca agttccgcga gacgcgtctc taccaggtcg ccccgatctc gacgaacctc
     7501 atcctctcct acagcgccga gcacgtgctc ggcctcccca ggtccttctg agagaaggat
     7561 aagacccttc cttatccggc gccggtcagc cagctgctga tgtgctgacg gctcgtcgtg
     7621 cgtaaggctc aaagcctcgt gcacgccgct gtagagacac tttaggcagg ttggatcttt
     7681 ccgcaagacg cctactcgag ttcggagagt atcctctcgt gggcttggag gtcggaggcg
     7741 ctgtggagaa cgaacctgat caggcgcacg tgctccaaac cgttggcctc ctcccgcatc
     7801 gtcccgagcg ccacccccgc cgcctcctcg accgggtagc cgaagatccc ggtcgagatg
     7861 gcggggaagg cgatggactc cacgctgttc tcctc
//
LOCUS       DASTKM010000121         8834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_556454, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000121 DASTKM010000000
VERSION     DASTKM010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556454"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1491
                     /locus_tag="VFQ10_05270"
     CDS             199..1491
                     /locus_tag="VFQ10_05270"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9964783.1"
                     /translation="MARSVSVRRSARVVVLGAGFAGASLLRHLPPALRRPGETLLIDR
                     REEYTFVPLIHEVAVGRVHPRTVSSPIAPICAGVCEFLHAEVSGIDLTQKTLETPEGP
                     VGYEYLVLNPGSVAAEPGPLLQDYFHSFWSLDDALRLRDAVGDAWRRATSPGPRPPQG
                     TLTVAIVGGGATGVEVAAEVAALFDYLGKRSHRRPSEVPRVVLLEATHRLMDWLDPYF
                     DSVARKTLSGLGVEVRLNTPVETASAEGVLAGEDWLKAATRIWTAGVEASPLVRGLPG
                     EHDAAGRSHVGEHLTLPDHPEVYVPGDAGVYTHPRLGPLPPTASVAVQQGPWVARDLK
                     RRLRGAESKRGRPPFRFFDRGYVVSLGPESAVADAVGLRLRGPAAQALYRSVLLYYMK
                     SRRDRILTGADWAMERTLGRLGFGSQEPSAVSKTKDGL"
     gene            complement(1658..1933)
                     /locus_tag="VFQ10_05275"
     CDS             complement(1658..1933)
                     /locus_tag="VFQ10_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET9964784.1"
                     /translation="MRKVCQIFVCATPGEGRCGAKGGGELFDMFRDEVERYGVAPSTV
                     LRNACTRRHEEGPVVFAYPDDVWYTRVTPEDVPEIVERHLAPMRVES"
     gene            complement(1982..3427)
                     /locus_tag="VFQ10_05280"
     CDS             complement(1982..3427)
                     /locus_tag="VFQ10_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HET9964785.1"
                     /translation="MESIPSASYSLTLRVEFPHEAGALGKILTTIGDADGMVGAVDIV
                     RMRRDRTTRDITVNARDSEHGQQVVQVVEDLPQVRVINVSDRTFLMHLGGKIEVRSKL
                     QIRTRDDLSMVYTPGVARVCRAIASDPERAFNLTVKRNTVAVVSDGTAVLGLGDIGPR
                     AAMPVMEGKAALFKQFANVDAFPICLDTKDTDAIVETVKNLAPAFGGINLEDISAPRC
                     FEVEERLKKDLDIPVFHDDQHGTAVVVLAALINSLKIVDKDLEDLRMVVNGVGASGVA
                     CAKIIMAAGAKNIVGCDSKGIVHEGREDLNESKRWFAENTNPEGRTGDLPDAVAGTDL
                     FLGLSVPDVLTVEHVESMNPDPIIFAMANPDPEIRPEVAMGKARIIATGRSDYPNQIN
                     NVLCFPGIFRGALDVRAREIDEPMKLAAAEAIASVIPAEDLSEDYIIPSVFDERVAPA
                     VAEAVANKAKETGSARRIQERAETGLKVTSF"
     gene            complement(3755..4207)
                     /locus_tag="VFQ10_05285"
     CDS             complement(3755..4207)
                     /locus_tag="VFQ10_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964786.1"
                     /translation="MDLQRLYAGSFIDWEAVAASWNKRTVPSRLLLFAARRYLDERGG
                     SVDSGRYGVLASAQLPEEIKRAFASPPERDAPTSRRWGEFVDAAVAAELEMVSYGERP
                     PLLHELRAGLNQAAAEAGPLTEKGRWFLARRDALPGTDLPDDPGYLPI"
     gene            complement(4389..6356)
                     /locus_tag="VFQ10_05290"
     CDS             complement(4389..6356)
                     /locus_tag="VFQ10_05290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET9964787.1"
                     /translation="MNEFFETSNKKSKRRPPGRFVRGLRALFNLVLVLSVIAVAMLAC
                     LYLFVVREYEDSLNRTYPELAENSYVYDIDGNKIGEIPVTERRETVGFEGLGAHLPEA
                     VVAVEDRRFYDHFGVDFEGLARAAWTDLRSWEVQEGGSTITEQLMKNLYVPEGQRLDT
                     SFRRRFVQSALAFAYERKHTKNEVLTAYLNTVYFGDGAYGAEEAAQRYFGKNARDLDL
                     SESAMLAGLLHAPSTYVTWEGDVIEGQVRERRDSVLRLMQEQGMISSREREEAEAQPM
                     KFAQEPPPQDPSYTPFLEKVGREVEEELGSRALRLGGLRIHTTMDPDLQHAAFETSKD
                     VLSEPDDPSAAVVTIEPQSGAIRSLAGQDGDFNLALDARRQPGSSFKPVVLAAALRED
                     ISPESTYISQDLAFDFQDEYYEVHNYDYVERGEISISDAMAESDNTVFVQLAVDVGLE
                     DVVKTAEELGITTPVEPYPSTAIGGLGSGVSPLDMASAYSTFAGGGIHREPYAIERID
                     RNSYGQSETVYDHRISGRRVLTGNEAAVATEVLRGVVEDGTATMFHDLDEEIGHPSAG
                     KTGTTDDFVDAWYVGYTPLLCTSVWVGYAEGRRSLVGVHGLQEPNGETLPMDIWSGYM
                     ASATAGDLALEFPEADAGELYTVEGGLSSGF"
     gene            6472..6780
                     /locus_tag="VFQ10_05295"
     CDS             6472..6780
                     /locus_tag="VFQ10_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964788.1"
                     /translation="MPVLSCTVRRVGDYESRTEEYEVAYAVGQAPPHGLHIRYQAARA
                     YEINAAIDAAVEVAMEAIAEADPDQRGPTEEALSKFSWRAIQSAKYEQGEPRSSSLEY
                     "
     gene            complement(6879..7640)
                     /locus_tag="VFQ10_05300"
     CDS             complement(6879..7640)
                     /locus_tag="VFQ10_05300"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0016860 - intramolecular oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HET9964789.1"
                     /translation="MGGFGLSDFTVYPAIDLRGGRCVRLKQGDFGRAKEYDADPLERA
                     REWMRQGAQAIHVVDLDGAEEGRPVQLELIGRIAMAVGIPLQVGGGVRSLEDLRAVRG
                     AGAQRVVMGTAAVEDRDLRLRAVEAAGDSVVVAVDASEGVVATHGWRRSSGVSVVDLS
                     KELIGDGVASVLYTDIARDGMNAGAALEGTATVAQIIPTVASGGVRGPEDVAALSRTP
                     GVLGVVVGTALYEGKVTLRELISSVQVPRSGMRKA"
     gene            complement(7622..8245)
                     /gene="hisH"
                     /locus_tag="VFQ10_05305"
     CDS             complement(7622..8245)
                     /gene="hisH"
                     /locus_tag="VFQ10_05305"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET9964790.1"
                     /translation="MTSLNVAIIDYDAGNTLSVARALEKVGAKVDLTSDPGRVGAADA
                     VVLPGVGAFGDCMRKLRKRGMDEACGEAFWSGKPFLGVCVALQVVFEASQESPGEEGL
                     GILEGNVVRLEGDGLKVPHIGWNEISLVRDHPVLQGLDGEVFYFVHSYYPLPEDTGDL
                     IGETDYGTRFCSVAGRENLVAVQFHPEKSSKAGLKLYENFLAWAGSV"
     gene            complement(8323..>8834)
                     /locus_tag="VFQ10_05310"
     CDS             complement(8323..>8834)
                     /locus_tag="VFQ10_05310"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HET9964791.1"
                     /translation="GEAEVATGVGFMDHLLHLLAHHSGMDLTVEAKGDTWVDDHHTVE
                     DTGLVLGRAFDEALGERGGIVRFADASAPLIESLSTAVVDLGARSHLTCNLGPMPEKV
                     GTFDVELFPEFLRAFTQYGRFTLHLNCHYGENAHHKIESGVKALAVAMRKGIAPRTSG
                     SASTKGVVD"
BASE COUNT         1546 a         2882 c         2761 g         1645 t
ORIGIN      
        1 cccggagggg tcgttccccg ttttcctgac accccttaga ccgccggcac cccatcagca
       61 ggcgatccgt gcagtctacg ttcgggcgcg cgttttgtaa agcgtctcgc cgagaagggg
      121 caccccgaac gatatactcc cggaagatga agggtgtagt gaaggaaaca gggacgtgcc
      181 aaagggaagc tctcatggat ggctcgtagc gtgagcgtca ggcgcagtgc gcgcgtggtc
      241 gtcctcgggg ccgggttcgc aggagcctcc ctgctcaggc acctccctcc ggccctgcga
      301 agacccggcg agaccctgct gatagaccgc agggaagagt acacgttcgt gcccctgatc
      361 cacgaggtcg cagtcggccg ggtgcatccc aggaccgtaa gctcacccat tgccccgatc
      421 tgcgccgggg tttgcgagtt ccttcacgcg gaagtctcgg gtatagacct cacacagaag
      481 accctcgaaa ccccggaagg ccccgtcggc tacgagtatc tggtcctcaa ccctggcagc
      541 gtcgctgcgg agcctggacc actgctgcag gattacttcc actccttctg gagcctggac
      601 gatgcgctgc gcctgcgcga tgccgttggc gatgcctggc ggcgtgccac gagcccaggg
      661 cccaggccgc cacagggcac tctgaccgtg gccatcgttg gcggtggggc gacgggcgtg
      721 gaggtcgccg ccgaggtagc agccctcttc gactatctag ggaaacgctc ccatcgcagg
      781 ccgtccgaag taccgcgtgt cgtgctgctc gaggctacgc accggctgat ggattggcta
      841 gacccctact tcgacagcgt cgcgaggaag acgctctctg gcctcggcgt agaagtgcgg
      901 ctgaacaccc ccgtagagac cgcgagcgcc gagggcgttc tcgccggcga agattggctg
      961 aaggcggcca cccgaatttg gacggccggc gtcgaggcct cacccctggt gagagggttg
     1021 ccaggggagc acgacgcggc gggccgctcg catgtgggcg agcaccttac cttgccggat
     1081 catcctgagg tctacgtgcc cggcgacgcc ggggtataca cccatccgcg cctcggtccc
     1141 ctgcccccca ccgcctccgt cgccgtgcag cagggaccgt gggtcgcacg ggacctcaag
     1201 cgccggttgc gtggggcaga gagcaagcgc ggcaggccgc ctttccgctt cttcgaccgg
     1261 ggctacgtcg tgagcctcgg ccctgagagc gccgtggccg acgccgttgg cctcaggctg
     1321 cgaggccctg cggcacaggc tctttacaga agcgtccttc tctactacat gaagagccgg
     1381 cgcgacagga tactcaccgg cgcagactgg gcgatggagc gtaccctcgg acggctcggc
     1441 ttcggtagcc aagaaccttc agccgttagc aagaccaaag atgggctgtg aggcatccca
     1501 ccaatgcgca atccagtaga ttcactcctg gagatcctac cttggtgaat tctcgcttgc
     1561 ccgggaatga aagtcataca tcgcgcaagt gatcttatcg attccaaatg tcagctgaag
     1621 tgctgacaag ctgaaaccgg agactggagt gtgctgacta gctctccacc cgcataggcg
     1681 ccaggtgacg ttctactatt tcgggcacgt cctcgggtgt tacgcgtgta taccagacgt
     1741 cgtcggggta ggcgaacacg acaggacctt cctcgtggcg gcgggtgcag gcgttgcgga
     1801 gtacagtaga gggcgccacg ccatagcgct ccacctcatc ccggaacata tcgaacagct
     1861 cgccgccgcc cttcgcgccg cagcgtccct cgcccggcgt ggcgcaaacg aagatctggc
     1921 acaccttcct catgcccaga ttctatgccg cagccagtag aagtccagac gcggtgggcc
     1981 tctagaagct ggtcaccttc aggccagtct cagcgcgctc ctggatgcgc ctggccgagc
     2041 ccgtctcctt ggccttgttg gcgaccgctt ctgcgacggc gggagcgacc cgctcgtcga
     2101 agacggaagg gatgatgtag tcctccgaga ggtcttccgc tgggatcacg cttgctatcg
     2161 cctcggccgc tgcgagcttc attggctcgt ctatctcacg ggccctgaca tcgagggcgc
     2221 ccctgaagat gccggggaag cagagcacgt tgttgatctg attcgggtag tcgctgcggc
     2281 ctgttgcgat gatcctcgcc ttccccatcg cgacctcggg tcttatctcg gggtcggggt
     2341 tggccatggc gaagatgatg gggtcagggt tcatgctctc gacgtgctcg acggtgagta
     2401 catctggcac cgagagcccg aggaagaggt ccgtcccggc gacggcgtca gggaggtcgc
     2461 cggtccttcc ctcggggttg gtgttctcgg cgaaccaccg cttggactcg tttaggtcct
     2521 cgcgcccctc gtggacgata cccttagagt cgcagccgac gatgttcttc gcgccagccg
     2581 ccatgatgat cttggcgcag gccacccccg aggcgccgac gccgttcacg accatgcgga
     2641 ggtcttctaa atctttatcc acgatcttga gggagttgat cagggccgcc agcactacga
     2701 cggccgtgcc gtgctggtcg tcgtggaaga cagggatgtc cagatccttc ttcagccgct
     2761 cttcgacctc gaagcaccgc ggggccgaga tgtcctccag gtttatgcca ccgaacgccg
     2821 gcgccaggtt cttgactgtc tccactatcg cgtccgtatc cttggtgtcg aggcagatcg
     2881 ggaacgcgtc cacgttcgcg aactgcttga agagcgccgc cttgccctcc atcacgggca
     2941 tcgcggcccg tggccctatg tccccgagcc cgaggacggc agtaccgtca gagaccacag
     3001 cgaccgtatt gcgctttacg gtaaggttga aagccctctc cgggtcgcta gctatcgccc
     3061 tgcagacgcg cgctacacca ggcgtataga ccatagagag gtcgtcgcgg gtgcggatct
     3121 ggagcttgga acggacttcg atcttacccc caaggtgcat gaggaacgtc ctgtccgaga
     3181 cgtttatgac cctgacctgg ggcaggtcct cgacgacctg gaccacctgc tggccatgct
     3241 cggagtcacg ggcgttgacg gtaatatcgc gggtggtcct gtcccggcgc atgcgcacga
     3301 tgtcgacggc gccgaccatc ccgtcagcat cccctatggt cgtcagaatc ttcccgaggg
     3361 ctcctgcctc gtgcggaaac tcgaccctca aggtgaggct gtagctcgca ctgggtatcg
     3421 attccataac tttgacctct ccttccttgg cccgccccac gcgtggcacg agaatgattg
     3481 tatgtcagca tggcgcttcg cgccttgtca gcacgcctcg ggcctttcag gccctcgctt
     3541 gtcagcttat tggttccggc cttgccctgc agcgcactaa ggacgattga ggcggaggtc
     3601 ggattccaaa cttgtcggaa ggctacggaa ggatttggct gaagtgctga gagccgccga
     3661 ccggagggag gtggcgtgct gacaagccca ggcgtagccg gagcgggctg acgagcgaag
     3721 cccgaagggc gtagtgtgct gaaatgctga tacctcaaat cggcagatat ccgggatcgt
     3781 cgggaaggtc ggtccctggg agggcgtcgc gcctggcgag gaaccatctg cccttctcgg
     3841 taagggggcc tgcctccgct gcggcctggt tcaggccggc ccgcagctcg tgcaagagcg
     3901 gcggtctctc gccgtatgag accatctcca gctcggcggc gacggcggcg tcgacgaact
     3961 cgccccagcg gcgcgaggtt ggcgcgtcgc gttctggtgg cgaggcgaag gcccgtttga
     4021 tctcctcagg gagctgggcc gatgccagga ccccgtaacg accggaatcg acgctaccgc
     4081 ccctctcatc gaggtagcga cgggcggcga agagtagtag ccgcgaggga acggtgcgtt
     4141 tgttccagga cgcggcgacc gcctcccaat ctatgaaaga tccagcatat agcctctgca
     4201 agtccataga gtcattctag atcagcattt cagcttgtca gcctgcgccc gcgcaggcta
     4261 gtgctgaaag cgtccgacag ggcgcgtgct gaaaggcgcg aagcgccgtg ctgtcaagcg
     4321 gaggtcggag ggtgctgtaa tgctgaccag cgcggcatcg ggaagggtga cccttcccga
     4381 tgccgctttc agaagccgct cgaaagccca ccctcgacgg tgtagagctc gcccgcgtcg
     4441 gcttcgggga actcgagcgc caggtccccg gccgtagcag aggccatgta acctgaccag
     4501 atatccatgg gcagggtctc tccgttcggc tcctgtaagc cgtggacgcc gactagtgag
     4561 cgacggccct ccgcataacc gacccacacg gaggtgcaaa gaaggggggt gtagccaacg
     4621 taccaggcgt caacgaagtc gtccgtggtc cccgtcttgc ccgctgaggg atgtcctatc
     4681 tcttcgtcca ggtcgtggaa catcgtcgcc gtcccgtcct ccactacgcc cctgaggacc
     4741 tcggtagcga ccgccgcctc gttgcccgtg agcacgcgcc gaccgctgat ccggtggtcg
     4801 tagaccgtct cgctctggcc gtagctgttc ctgtcgattc tttcgattgc gtaaggctcg
     4861 cggtggatac cgccaccggc gaaggtcgag taggcagagg ccatatcaag tgggctgacg
     4921 cctgacccca ggccgcctat agcggtcgac gggtagggct cgacgggcgt ggtaatgccc
     4981 aactcttcgg ctgtcttcac gacgtcttcc aatcccacgt cgacggcaag ctgcacgaac
     5041 accgtattgt ccgactccgc catggcgtcg gatatggaga tctctccccg ctcgacgtag
     5101 tcgtagttgt gtacttcgta atactcgtcc tggaagtcga aggccaggtc ctgggatatg
     5161 taggtgctct ccggagagat gtcttccctg agggctgcgg cgagcactac gggcttgaac
     5221 gagctaccgg gttgacgcct ggcgtcgagc gcaaggttga agtccccatc ctgtccggcc
     5281 agagaccgta tggccccgct ctgaggttct atggtcacta cggcggcgga agggtcgtcc
     5341 ggttccgaga ggacgtcttt cgaggtctcg aaggcagcgt gctggaggtc gggatccatc
     5401 gttgtgtgga tcctcagtcc tccgagccgt agcgcccgcg aacccagctc ctcctccacc
     5461 tcgcgtccga ccttctccag gaaaggcgtg tacgacggat cttgcggcgg cggctcctgg
     5521 gcgaacttca tgggttgggc ctcggcttcc tcacgctctc gggaggagat cataccctgt
     5581 tcctgcataa gtctcaggac gctatcgcgg cgctctcgga cttgcccttc gataacgtca
     5641 ccctcccagg tcacgtaggt cgaaggggcg tggagcagcc cggcaagcat ggccgactcc
     5701 gagaggtcga ggtcccgggc gttcttgccg aagtagcgct gcgccgcctc ctcagcgccg
     5761 taggcaccgt ccccgaagta cacggtgttc aggtaggcgg taaggacctc gttcttggtg
     5821 tgcttgcgct cgtaggcgaa agcgagcgca gactgcacaa agcgtctgcg aaaagaggtg
     5881 tcgagacgct ggccctcggg aacatagagg ttcttcatga gctgctccgt gatcgtggag
     5941 ccaccctcct gcacctccca ggatctcaag tcagtccagg ccgcgcgggc gagcccttcg
     6001 aagtcgacac cgaagtggtc gtagaagcgt cggtcctcga cggcgacgac ggcctcgggg
     6061 aggtgcgcgc cgagaccttc gaaaccgacg gtctcccgcc tctcggtgac cgggatctcg
     6121 cctatcttgt tcccgtcaat gtcgtagacg taggagttct cggcgagctc cgggtatgtc
     6181 cggttgaggc tgtcctcgta ctccctgact acgaacaggt agaggcaggc tagcattgcc
     6241 acggcgatca cgctgaggac gagaacgagg ttgaagaggg cgcgtagtcc ccttacgaag
     6301 cgtccgggcg ggcgcctctt cgacttcttg ttgcttgttt cgaagaactc gttcacgacc
     6361 ctccggccgt agtctacccc aatcgtgtct ggggtgatcg acgtagggcc atggtcacaa
     6421 agcgggcgcc ccgtcccccg gtggcatcca aagggatggt aggatgcgcg catgccggtg
     6481 ttgagttgca cggtcaggcg tgtcggtgac tacgagagcc gaacggagga gtacgaggtc
     6541 gcctacgctg tagggcaggc gcccccgcac gggttgcata tcaggtacca agccgcacgg
     6601 gcttacgaga taaacgccgc catcgacgcc gcggtggagg tggcaatgga agccatcgcc
     6661 gaggctgacc ccgaccagag gggtccgaca gaggaagccc tctccaagtt ctcctggcgt
     6721 gcgatccaat ccgccaaata cgaacagggc gagccccgtt cgtccagcct cgaatactga
     6781 gagcaagtca gcccgctacg gccttcgctt gtcaaccagg tctcgcacgc gcaaacggtg
     6841 aaggccttac aagttgatag ccccgtcgat cgtagggctc aggctttccg cataccgctt
     6901 cgaggaacct gcacgctcga gatcagctct cggagtgtaa cctttccctc gtagagcgcg
     6961 gtgccgacca ccacgccgag tacgccgggg gttctggaca gcgcggccac gtcctctggg
     7021 ccgcggacgc ccccgctggc cacggtgggg attatctgcg ctacggtcgc ggtcccttcg
     7081 agggcagccc ctgcgttcat gccgtcgcgc gcgatatcgg tgtagagaac cgaggcgaca
     7141 ccgtcaccta tcagctcttt ggagagatcc acgacggata ctccactcga cctccgccaa
     7201 ccgtgtgtcg cgacgactcc ctcgctggcg tcgacggcca ccaccacaga gtcgcctgcc
     7261 gcctcgaccg ccctgagccg caggtccctg tcttccaccg ccgcggtgcc cattacgacg
     7321 cgctgggcac ctgcgccccg tacggcgcgc aggtcttcta aggaacggac tcccccccca
     7381 acctggagcg ggatgccaac cgccattgcg atacggccaa tgagctcgag ctggactggt
     7441 ctgccctcct cggcgccgtc caggtccacg acgtggatcg cctgagcccc ctgtctcatc
     7501 cactcgcggg cgcgctcgag ggggtcggca tcgtactctt tggcccgccc gaagtccccc
     7561 tgcttcagac gcacgcatcg accgccgcgc aggtcgatag cgggatagac ggtaaagtca
     7621 ctcaaaccga acccgcccac gccaggaaat tctcgtacaa cttcaggcct gccttgctgc
     7681 tcttctcggg atggaactgg acggccacga ggttctccct tccggccacg gaacagaagc
     7741 gcgtcccgta gtccgtctcg ccgatgaggt cacctgtatc ctcgggcagg gggtagtagg
     7801 agtgcacgaa gtagaacacc tcaccgtcga ggccctggag cacagggtga tcccgcacca
     7861 gagatatctc gttccacccg atgtgcggga ctttgagccc gtccccctca aggcgcacca
     7921 cgttcccctc caagatcccg agcccctcct cccctggcga ctcctgggag gcctcgaaga
     7981 cgacctgcag cgcgacacac accccgagga atggcttgcc cgaccagaac gcctccccgc
     8041 acgcctcgtc catcccgcgc ttgcgcaact tcctcatgca gtctccgaaa gcccctacac
     8101 ctggcagaac cacagcgtcg gccgcgccaa cccgccctgg atcggatgtg agatccacct
     8161 tcgcaccgac cttctccagc gcccgcgcga cggagagtgt attccccgca tcgtaatcga
     8221 tgatcgctac gttcaagctc gtcaccattt cagctactca gcatgtgcgt tcgcgacccc
     8281 ctctgaaaag gcggcgcagc cgccgggctg acgtgctgac gatcaatcga ctaccccttt
     8341 cgttgaagcg ctgcctgagg tgcgcggggc gatgcccttc ctcatggcaa cggccagcgc
     8401 cttgacgcct gactctatct tgtggtgcgc gttctcgccg tagtggcagt tgaggtgtag
     8461 cgtgaagcgc ccgtactggg tgaaggcgcg caggaattcg gggaacagct ccacatcgaa
     8521 ggtccccacc ttctccggca tcgggccgag gttgcacgtg aggtggctcc tggccccgag
     8581 gtcgacgacg gccgtcgaga gcgactcgat gaggggcgcg ctcgcgtcgg cgaagcgcac
     8641 gatgcctccc ctctcgccga gcgcctcgtc gaaggcgcgt ccgagcacca gccctgtgtc
     8701 ctcgaccgtg tggtgatcat ccacccacgt atcgcccttg gcctctaccg tgaggtccat
     8761 gccggagtgg tgggcgagca ggtgcaggag atggtccata aaacctaccc ccgtcgcaac
     8821 ctcggcctct ccgc
//
LOCUS       DASTKM010000122         9445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1262728, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000122 DASTKM010000000
VERSION     DASTKM010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262728"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFQ10_05315"
     CDS             complement(<1..413)
                     /locus_tag="VFQ10_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HET9964792.1"
                     /translation="MKRSLDIALRYGVAVLAAGAALGIKLLLDPLTVQDTPFLLVFGA
                     IIVSAWYGGLGPGLLATAVSALATDYFFLYPRGTITGFSVEGIDVLAFTLEGVLVSVL
                     TSSLRSARDRAQRSTLEARSHQESLRESEERFRLMV"
     gene            complement(646..942)
                     /locus_tag="VFQ10_05320"
     CDS             complement(646..942)
                     /locus_tag="VFQ10_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964793.1"
                     /translation="MRRRPGKQERLAGLFVVISATFVFAPAIWLLQRWSEAVARDPAD
                     AWGARWLGEHLTAHPYLTAGVVFLSIVLASGVVMLILVSLAKRLAVRLAGRRQQ"
     gene            complement(1007..1666)
                     /locus_tag="VFQ10_05325"
     CDS             complement(1007..1666)
                     /locus_tag="VFQ10_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964794.1"
                     /translation="MSAREAVIEEIQGAFAGNVHPGGRFLQGSFEGCEPYEEVGPFEN
                     EEDWRSIDAGFLDGHANALSFFSEAGFRFFLPAYMISDLRGQLQTADPLFHLTHGFSD
                     WSTEVPAGERTFVIRHGGSAFINPRRYGAMTSYDYARWRLSVFTREEANAIVAYLEFK
                     RDLDPDVIDRTAIDRALKSFWLERARTAPQARSLQQHIAEQEEYLAAIRPDIEEGTGE
                     Q"
     gene            2030..4864
                     /locus_tag="VFQ10_05330"
     CDS             2030..4864
                     /locus_tag="VFQ10_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9964795.1"
                     /translation="MAILVGCIVFIGWALDVGALSDILPGPVAMNPLSAIGLLLAGVS
                     AWLLQDEHVEGRERWISRVCALVVALVGLIRLIQVLFGLEPGIDQFLFSASLASEAAL
                     TGFSNRMASDTALDFVLMGGALFYLDRRSRRGRWVSQYLALSVFVFSILAFAGYVYGV
                     ANLYGNTSYIPIAAMTFVVFSAGVLCARPERGLMAVVTSRNMGGVVARRLLPAAILVP
                     LVLGWFRLRGQQVGLYGTELGVSLFTVATMFIVAALVWWCARLLYRVDGERKLAEEEM
                     RAAREMAEEANRAKSEFLANMSHEIRTPMNGVIGMTDLLLDTHLDEEQREYAETVHFS
                     SQTLLAILNDILDFSKIEAGSLSLETINFDLRREVEEVAALLAGHAHEKGLELVSFVE
                     PGTPTAVRGDPFRLRQVLTNILGNAIKFTEEGEVTLQAQLVEDAPDTATVCFEVRDTG
                     IGMTEVQRSRLFKPFTQADASTTRRYGGTGLGLAISRQLVLMMGGEIGVRSEPGVGST
                     FWFTTRLQKRPEDGRATPVPRAGLQDLRVLIVDDHETNRRILHKQITSWGMRDGMAEN
                     GAEALDLLRAAAESGDAYDLAVLDMQMPDMDGIQLAHAISAEPAISSTRLVLLTSIGI
                     NINETARMAGVEVVLNKPVRQSQLHDALATMLGTPTEAQGRPSRGGVRSTAHATPEAE
                     LHASRGHVLLVEDYPVNQVVATRMLERSGYRVDAVNNGREAVEALSNIPYGAVLMDVQ
                     MPEMDGYEATAEIRRREGSVRHTPVIAMTADAMQGDRERALAAGMDDYIAKPVKHEEL
                     EAVLGRWIPQPEQEPPAHTRDEDSGTAEDTPALDLSVLESRRGPQRDGEPDKLARIVG
                     LFIEDVPLRLDELRQAVERGEAQKVEETAHMLKGGSGYMGAVQMAQICAGIQGLGASG
                     ELSHAPQLLDDLEAEFKRIRPALEAAVTAN"
     gene            4878..5708
                     /locus_tag="VFQ10_05335"
     CDS             4878..5708
                     /locus_tag="VFQ10_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964796.1"
                     /translation="MTTGAKSLSTLAVALALFGLAGSAVMVAVPGLFAGPVRPEFTVF
                     LVAGVAAIVLTAGFFGYIYRLGMGFGRTVLVLAAGYNALIAAVKLGLAPAALYQANRE
                     QAFDAAFGDPNELWFYLGVGSGVLLLYLLVFGVMYSVFRRRFRRRSLPSEPPPEQRGR
                     WSNRTIVVGVVVLVAIAASFLWIMPVVYVGLPTFSYLFYIFTTFGAAIALALVLAAVL
                     AYKAFDEIEKRAVRLGDATLLASFFWLGLALILLYHVMWIVFFLTLVSIWPFRTYTPK
                     "
     gene            5717..6691
                     /locus_tag="VFQ10_05340"
     CDS             5717..6691
                     /locus_tag="VFQ10_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET9964797.1"
                     /translation="MLALYWSLLRYRVAVMIVLFMLLGAAWHRGLTAIDAPLILTILA
                     LASSYVSATSVNDISDREIDEINHPGDKGRPLVTGVARTTDLWVLFVVSSVLAVALGL
                     ATGPGAAGIMLLSVVIGALYSLPPAMLSYRTFLAPLTLAVAYVGIPYWAGVVVVGESL
                     LLSDLSLMLSLYLLFAGRIILKDFRDREGDAAFGKPTFFLKYGKKATCLASLCALCAG
                     DALLAASLADRWWLAALLQLYVVSIAIMLYRLYESSSRKDEQLSIGVGAKMGNGLLVT
                     LLGALILSNQGADWITQFIFGMALTAIYVLNFVDFLRHPERAVVGYKG"
     gene            6700..7467
                     /locus_tag="VFQ10_05345"
     CDS             6700..7467
                     /locus_tag="VFQ10_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964798.1"
                     /translation="MRHLSERGLTYSLVGAAMLVALIFLLPLVDTMPESIFAARITAY
                     ALVLVPISLLLHGFFVAAGGYATPRLLALGAVSAALGLSTAFLARPEDLKSSSGVFVL
                     LALSVADLSRILAAACVGIALARYITSVGVVLIIVLAAIASDLFSVFAGPTRVLVQED
                     SPVLDGLLLVFPTFGSALGFGLGVSDFIFLALFAAASRFLDLRYAATLLGVCFAAFLA
                     VTAGLLLQRPLPALPFIAVTFVIVNADLIVTSLVKRR"
     gene            7625..8692
                     /locus_tag="VFQ10_05350"
     CDS             7625..8692
                     /locus_tag="VFQ10_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9964799.1"
                     /translation="MPIAERILLIVNRSAGIGHGDPVVGRVRAMLVKLLGEQATLMVE
                     VVTDHPEARTRAKQFMDASDVPGLILVGGGGGTLRAVIEGLCEGSEEGRLPGRERVLI
                     GALRMGSGNVLARQFGIPRDTEAGLRGIIDNLRAGRTVPCCVMRCETGARGGLTEVHY
                     GATLGGFGQFGRVPGDLVRWERRFPAAHKVAATFFGIERLTNVEYALALLIRSASAAL
                     LGGSTAEKVEVRTSDRSEVMRLLAGVAMNFPFEAFPVDPGVRAEDEALSLYLVPYSGR
                     LSTLLLVVDPRRLLRDALKIRIEGQERVEIRLLDRNTAEFFLDEDPMTYHGRLTLQVA
                     GSLAFVPGPEYRFRAGSGALA"
     gene            8689..>9445
                     /locus_tag="VFQ10_05355"
     CDS             8689..>9445
                     /locus_tag="VFQ10_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964800.1"
                     /translation="MSLQVCILGIDGSGKSTISAVLPAILAAELGLVAGAAGETFRVS
                     DPDEDHLAPKFHPDGLPVSARLTTRFKRKAKRLVDSRSLYPAFKIAQMITQDIAAAAL
                     ARRYRARVIVSDGNALLSATGRAANYLRAASDGGHKPAPSAEDLRAVFSYIIEGTPIP
                     SETQRRLPRLQRARAIYKLAGRLGLRAVWLPDVVIFLDLPPEISIARIASRGKKVDRH
                     ENLADLAQAREMYLKSLEAFGRYRSFEATYRIPL"
BASE COUNT         1665 a         2948 c         2933 g         1899 t
ORIGIN      
        1 accatcagcc tgaagcgctc ctcgctctcg cgcagactct cctgatggct cctggcctcc
       61 agcgtgctcc gctgggcccg atccctggcc gagcgcaacg aggaagtcag cacactgaca
      121 agtacccctt cgagcgtgaa agcgagcacg tctatcccct cgacgctgaa tcccgtgatg
      181 gtgcccctgg ggtagaggaa aaaataatct gtcgccaggg cgctaacggc ggtggctagc
      241 agaccgggtc ccagcccgcc gtaccaggcg ctgacaatta tggcaccgaa gaccagaaga
      301 aacggcgtgt cctgcacggt cagaggatcc agcagcagtt tgattcctag ggcagcaccg
      361 gcggccagca ccgccacgcc atagcgcaga gctatgtcaa gagagcgttt catggaaacc
      421 tgtcatcctg cttccgcgcc ctcgccccag agagcaggct cgcgacacat gcaatggctg
      481 gtttcccttt cacctgccgt gtccgagtcc tgcatccggc gttttcggcg ctgtcggctt
      541 tacaggatat cgtggtcccc tctgacacac taattggtgg atcggattat acgttatgtg
      601 ttcatcgcaa ggcgggaatg acggttcccc gcgacttaac gatgcctact gctgcctgcg
      661 gcccgcgagt cggaccgcca gccgttttgc gaggctcacc aagatcagca tgaccactcc
      721 gctcgcgagc actatgctga ggaagacaac acctgccgtc aggtacggat gagccgtcag
      781 gtgttcgccg agccagcggg cgccccaggc gtctgccggg tcacgggcca ccgcttcgct
      841 ccagcgctgc aggagccaga tcgcgggtgc gaaaacgaag gtggcactga tgacaacgaa
      901 gaggccggca agccgttcct gtttacctgg tctcctgcgc atgggtgatc cccgcctgat
      961 ctctgccttc agccgtccgc gagaagcatc cgggctgggt caacgttcat tgttcaccag
     1021 taccttcctc gatgtcaggc ctgatcgcgg cgaggtattc ctcctgctcc gctatgtgct
     1081 gctggaggct cctggcctga ggcgccgtcc gtgccctctc caaccagaac gacttcaggg
     1141 ctctatcgat agccgtcctg tcgatgacgt cggggtctag atctcgtttg aactccaggt
     1201 aggcgactat ggcgttcgct tcttccctgg taaagaccga caggcgccat cgcgcgtagt
     1261 cgtaagaggt catggcacca tatctacgcg ggttgatgaa cgccgacccg ccgtgcctga
     1321 tcacgaaggt acgttccccg gcgggaacct cggtgctcca gtcggagaac ccgtgcgtca
     1381 gatggaacag gggatcagcc gtctgaagct ggccgcgcag gtcggagatc atatacgccg
     1441 ggaggaagaa cctgaagccc gcctccgaga agaaactgag cgcgttcgca tggccgtcca
     1501 gaaagcctgc atcgatactc ctccaatcct cctcgttctc gaagggaccc acctcttcgt
     1561 aaggctcgca cccttcgaaa ctaccctgca ggaaacgccc tcccggatgc acgtttcccg
     1621 cgaaagctcc ctgaatctcc tcgatcacag cttctctggc gctcatcctg actcccgaaa
     1681 agttcgcagg cctaccgtct ttcactattg tgtaccattg ctgaaggcct gtggggacgg
     1741 cactcaacac tcagcgaatc gagccgatac ctgatgcctg agagctgtag cctgagacct
     1801 tactctgcgt gagccacgtt gaactctaga gtacaatggc atccggttgt tgtgaatcct
     1861 ggcttggatg gggtcttttc agcagcccgg ttggtgtagg gaacagccgg tacgggagta
     1921 cgagatagcc gggcaaagaa acgagacggc ggggcgaccc gggctggggg gcgactccat
     1981 cccaggtctt gttacctcgc tcgagacttt ctcgcgagct tcgagcatcg tggccatact
     2041 ggttggctgc attgttttca ttggctgggc gctcgatgta ggggccttga gtgacatcct
     2101 gccaggtcct gtggcgatga accctctctc ggcgataggt ttgttgttgg ccggtgtgtc
     2161 ggcctggttg ttgcaggatg agcacgtgga agggagggag cgttggatct cacgggtatg
     2221 cgccctcgtg gtggcgcttg tcggcctgat caggctgatc caggtgctct tcggcctcga
     2281 accgggcatc gaccagttcc tgttttcagc gagcctggcc tcggaggcgg cgctaacggg
     2341 cttctccaac aggatggcgt ccgacacggc gcttgacttc gtcctgatgg gtggcgcgct
     2401 gttttatctg gacaggagga gccgccgtgg tcgctgggta tcccagtacc tcgccctgag
     2461 cgtgttcgtg ttctccattc tggctttcgc cggctatgtt tacggcgtcg cgaatcttta
     2521 cggcaacacc tcctacatcc ccatagcggc gatgactttc gtcgtgttct cggccggcgt
     2581 cctgtgcgcg cgccccgagc gcggccttat ggccgtcgtc acgagccgca atatgggggg
     2641 tgtggtggcg cgccgtctgt tacccgccgc catcctggtt ccgctggtgc tcgggtggtt
     2701 caggctcagg ggacaacagg tcggcctcta cggcacagaa ctcggggtgt ccctcttcac
     2761 cgtggcaacc atgttcatcg tcgccgccct ggtgtggtgg tgcgcccgcc tgctctaccg
     2821 ggtcgacggc gagcgcaagc tggccgagga ggagatgcgg gcggcgaggg agatggccga
     2881 ggaggccaac cgcgcaaaga gcgagttcct ggcaaacatg agccacgaga tccgcactcc
     2941 catgaacggc gttataggca tgacagacct ccttctggac acccacctcg acgaggagca
     3001 acgcgagtac gcggagacag tccacttctc gagccagact ctgctcgcca tcctcaacga
     3061 catccttgac ttctccaaga tagaggccgg cagcctgagc ctggagacca taaacttcga
     3121 cctgaggagg gaggtcgaag aggtcgccgc cctgctcgct ggacatgccc acgagaaggg
     3181 cctggaactg gtcagcttcg tggagccggg tacgccgacc gctgtgcgcg gcgatccctt
     3241 ccgtctgcgg caggtcctca ccaacatcct gggcaacgcg atcaagttca ccgaagaagg
     3301 ggaggtgacc ttgcaggccc agctcgtcga ggacgctcct gatacggcca cggtgtgttt
     3361 cgaggttcgt gacacaggca taggcatgac cgaagtgcag cgatccaggc tcttcaaacc
     3421 ctttacccag gccgacgctt cgaccacccg ccgctacggg ggcacagggc tcggtctggc
     3481 catcagcagg caactggtgt tgatgatggg aggggagata ggcgtgagga gcgaacccgg
     3541 cgtaggctcc actttctggt tcaccacgag gctccagaag agaccggagg atgggcgggc
     3601 gactcccgtc ccccgcgcgg gcctgcaaga ccttcgcgtg ctcatcgtgg acgaccacga
     3661 gaccaaccgc cgcatactgc acaagcagat cacctcctgg ggcatgaggg acggcatggc
     3721 ggagaacggg gcggaggcgc ttgacctact gcgggccgcg gccgaaagcg gggacgctta
     3781 cgacctggcg gtcctggaca tgcagatgcc agatatggac ggcatccaac tcgcccacgc
     3841 cataagcgca gaacccgcca tctcttctac caggctggtg ctgcttacct ccataggcat
     3901 caatatcaac gagactgccc gcatggccgg cgtggaggtc gtcctcaaca agccggtgcg
     3961 ccagtcgcag ctccacgacg ccctcgcgac gatgctcggc acccctacag aggcacaggg
     4021 cagaccgtcg cggggaggcg tgagatctac tgcgcacgca acaccggagg ctgaactcca
     4081 cgcgtcccgc ggtcacgtcc tcctcgtcga ggactacccc gtaaaccagg tggtggcgac
     4141 aaggatgctc gagagatcag gctacagggt agacgccgtg aacaacggga gggaggccgt
     4201 cgaggccctc tcgaatatcc cgtacggggc ggtgctcatg gacgttcaaa tgcccgagat
     4261 ggacggctac gaggccacgg ccgagatccg gcgtcgcgag gggtccgtcc gtcacacccc
     4321 ggtaatagcc atgaccgccg acgccatgca aggggatagg gagagagcac tggccgcagg
     4381 catggacgac tacatagcga aaccggtaaa gcatgaggag ctggaggccg tgctcgggcg
     4441 ttggatccca cagcccgagc aggaaccccc cgcgcacacg agagacgagg actcaggaac
     4501 cgccgaagac actcccgccc tcgacctatc cgtgctggag agccggcgcg ggccccagag
     4561 agatggggaa ccggacaaac tggccaggat cgtcggcctt ttcatcgagg acgtgccgct
     4621 acggctggat gagttgcggc aggcggtcga gaggggagag gcccaaaagg tggaagagac
     4681 ggcccacatg ctcaaaggcg gctccggtta catgggagca gttcaaatgg cacagatctg
     4741 cgccgggatc cagggtctcg gcgcctccgg cgaactgtcg cacgcccccc agctgcttga
     4801 cgatctcgaa gcggaattca aacgcatccg ccccgccctg gaagccgcag tcacggcgaa
     4861 ttgagccagt cgacgacgtg actacgggtg ccaagagcct ttcgaccctg gctgtggcgc
     4921 tggcgctatt cggtttggcc ggctctgccg tgatggtagc ggtcccaggg ctctttgccg
     4981 gtcccgtgcg gccggagttc acggtcttcc tggtagccgg ggtggcggcc attgtattga
     5041 ccgccggctt cttcggctac atctaccggc ttggcatggg cttcggtaga acggtcctgg
     5101 tgctggccgc cggctacaac gccctgatag cggcggtgaa gcttggcctg gcgccggccg
     5161 cgctgtatca ggccaaccgc gagcaggcgt tcgacgccgc gtttggagat ccgaacgaac
     5221 tgtggttcta cctcggggtc ggctcgggcg tcctcctgct ctacctgctc gtcttcgggg
     5281 tgatgtacag cgtcttcagg cgacgtttca ggcgacgctc gctcccgtcc gaacctccgc
     5341 cggagcagag gggccggtgg tcgaacagga ctattgtggt cggcgtcgtg gtcctcgtcg
     5401 ctatcgccgc cagcttcctc tggatcatgc ccgtggtcta cgtcgggtta ccgaccttct
     5461 cctacctctt ctatatcttc acaacgttcg gtgctgcgat tgcgctggcc ctggtcctgg
     5521 ccgccgtgct ggcctacaag gctttcgacg agatcgagaa gcgggccgtc cgtctgggag
     5581 acgccaccct tctggccagt ttcttctggc tgggtctggc cctgatcttg ctctaccacg
     5641 tcatgtggat cgtcttcttt ctgacgctgg tcagcatctg gcccttccga acatatacgc
     5701 ccaagtaagc atgaagatgc ttgccctata ctggagcctg ctgcgctatc gcgtggctgt
     5761 aatgatcgtc ctcttcatgc tgctcggggc ggcgtggcac agggggctga cagctatcga
     5821 cgcgcccctg atcctgacga tcctcgccct tgcgtcgagc tacgttagcg cgacgagcgt
     5881 gaacgacatc tcggacaggg agatcgacga gataaaccac cccggcgaca aaggaaggcc
     5941 cctcgtaacg ggcgtagcca ggaccacaga cctgtgggta ctgttcgtgg tgtcgagcgt
     6001 ccttgcggtg gcactcgggc tcgccaccgg tcctggcgcc gcgggcatca tgctgttgtc
     6061 cgtcgtgatc ggggcgctct attctctgcc cccggccatg ctgtcgtaca ggacgtttct
     6121 ggcgccgctg acactggccg tggcctacgt cggcatccct tactgggccg gcgtcgtggt
     6181 agtcggggag agcctcttgt tgtcagacct gtccttgatg ttgtccctct atctgctctt
     6241 cgcggggagg atcatactca aagatttcag ggacagggag ggtgacgccg ccttcggtaa
     6301 gccgacgttc ttcctgaagt acggcaagaa agcaacctgc ctggcaagcc tctgcgctct
     6361 gtgcgccggg gatgctctgc tcgccgcatc cctggcagac aggtggtggc ttgcggctct
     6421 gttgcaactt tacgtcgtaa gtatcgccat tatgctctac cggctctacg aatcgagcag
     6481 ccgcaaggac gagcagttat ccataggggt tggggccaaa atgggcaacg ggctcctcgt
     6541 aaccctgctg ggtgctctta tcctatcgaa ccagggcgct gattggatca cgcaattcat
     6601 cttcggcatg gccctgacgg ccatctacgt gctgaacttt gtagacttcc tccgccatcc
     6661 tgagcgggcc gtcgtcgggt acaaaggata gggtgcgttt tgagacatct ctccgaacgg
     6721 ggactcactt attcgcttgt tggcgcagcg atgctggtgg ccctgatctt cttgttgccc
     6781 ctggtagata cgatgccgga atctatcttc gccgcccgga tcactgctta tgccctggtc
     6841 ctagttccaa tatctctctt gctccacggg ttcttcgtcg ctgcgggggg ttacgccacg
     6901 ccccggcttc tggcactcgg tgctgtgtcc gccgctctcg gcctctctac agccttcctc
     6961 gctaggcccg aggacctgaa gagtagttca ggggtctttg tcctgctcgc gctctctgtt
     7021 gccgaccttt cgaggatcct cgcggctgcg tgcgtcggca tcgcgctcgc ccgctacata
     7081 acctccgtcg gcgtggttct gatcatcgtc ctcgccgcga tagcctcgga cctcttcagc
     7141 gtcttcgcag gaccaaccag agtgctcgtg caggaggatt ctcccgtact cgacggcctg
     7201 ctgctcgtct tcccgacgtt cggatccgcc ctgggtttcg gcctcggcgt tagtgacttc
     7261 atatttctgg ccctgttcgc cgcggcgagc cggttcctgg atctccgcta tgcggcgacc
     7321 ctgctcggcg tctgcttcgc cgcgttcctc gccgtaaccg cgggcctcct tctccaacgc
     7381 cccctgccag ccctgccctt catcgccgtc accttcgtga tcgtcaacgc tgacctgatc
     7441 gttacctctc tggtgaagag acgctaggtc agcatcttca gccagtcagc acgacggcat
     7501 ttcagcctgt tgttttcgct ggtgccggaa gcctgtctcc aggatgttct agaattgctt
     7561 ctattgagtg ggagaagatc acacgcagac aaggggagaa tattcatgaa atggcaccca
     7621 ttctgtgcca atagctgaaa gaatcctgct gatcgtcaac cgcagtgccg ggatcggcca
     7681 cggtgaccct gtcgtaggca gggtccgggc gatgctcgtc aagctgttgg gtgagcaagc
     7741 gacgttgatg gtggaggttg tgacagatca ccctgaagct cgaacgcgcg cgaaacagtt
     7801 catggatgct tcagacgttc ctggcctgat actcgtagga ggcggcggtg gaacgttgcg
     7861 agccgtgatc gaaggtcttt gcgagggcag tgaggaggga cgtctgccag ggcgcgagcg
     7921 agttctcata ggagctttgc gcatgggatc ggggaacgtc ctagccaggc agttcggcat
     7981 accgcgcgac accgaggccg gactgcgggg catcatcgac aaccttcgag ccggccgtac
     8041 cgttccgtgt tgcgtgatgc gttgtgaaac cggtgcgcgc gggggcctta cggaggtgca
     8101 ctacggcgct acactaggcg gattcggtca attcggccgt gttcccggag accttgtccg
     8161 ctgggagcgc aggttcccgg ctgcccacaa ggttgcggcc acgtttttcg gtatcgagag
     8221 gctgacgaac gtcgagtacg ccctcgccct gctcatccgc tccgcctcgg cggctctgct
     8281 gggcggctcc acggcggaga aggtggaagt gcgcaccagc gaccgaagtg aggtcatgcg
     8341 gctccttgcg ggagtcgcca tgaacttccc attcgaggcg tttcccgtcg atccgggtgt
     8401 gcgtgcggaa gacgaggcgc tttcgctcta tctggttcct tacagcggca ggttgtcgac
     8461 actcctgctg gtcgtcgacc cccgccgtct cctccgggac gcgctgaaga ttcggatcga
     8521 gggccaggaa cgcgtcgaga tccggctgct ggatcgtaac accgcggagt tcttcctcga
     8581 cgaggacccg atgacctacc acggccggtt gaccctgcag gttgcgggct ccctcgcttt
     8641 cgtgccaggg cctgagtacc gattccgagc aggaagcggg gcgctcgcgt gagcctccag
     8701 gtttgcatat tggggatcga cgggagcggt aagagcacca tctccgcagt cctacccgcc
     8761 atcctggccg ctgagttggg cctcgtcgcg ggagcggcgg gagagacgtt ccgggtctcc
     8821 gatcccgatg aggatcatct cgcgccgaag ttccacccgg acggcctgcc ggtctccgcg
     8881 cgcctcacca cgcggttcaa gcggaaggcc aaacggctgg tagatagccg cagcctttac
     8941 ccggcgttca agatcgcgca gatgatcacc caagatatcg ccgccgcagc attggccagg
     9001 cgttaccgcg ctcgcgtaat agttagcgac ggtaacgctc tgttgtcggc cacgggacgc
     9061 gcggcgaact acctgcgcgc ggccagcgat gggggccaca aaccggcgcc gagtgccgaa
     9121 gacctgagag cggtgttctc ttacatcatc gaaggtacgc ccataccatc agagacccag
     9181 cgcaggcttc cgcgtctcca gagagcgaga gccatctaca agctggccgg acgtctcggc
     9241 ctccgcgcgg tctggttgcc tgacgtcgtc atattcctcg atcttccgcc cgagatctcc
     9301 atcgcgcgca tcgcatcgcg aggaaagaag gtcgaccgcc acgagaatct ggcggatctc
     9361 gcccaggcgc gcgagatgta cctcaaatct ctcgaagcgt tcgggcggta ccgctctttc
     9421 gaggccacct atcgcattcc gctgg
//
LOCUS       DASTKM010000123        11141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_380757, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000123 DASTKM010000000
VERSION     DASTKM010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380757"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..905)
                     /gene="nth"
                     /locus_tag="VFQ10_05360"
     CDS             complement(273..905)
                     /gene="nth"
                     /locus_tag="VFQ10_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET9964801.1"
                     /translation="MSLSAAPASEVTARLKAEYPDARTELDWSNPLELLIATILSAQT
                     TDMQVNRVTESLFAKYRTAEDYADSNPEELEEDIRPTGFYRNKARSLRGMANALVEKH
                     AGEVPRTMSELVALPGVGRKTANVVLGNAFGTNEGIVVDTHVRRVSGRLGLTESSDPV
                     NIEQELMEVVPEEDRTIFSHLLILHGRRTCKARKPDCPNCILNDICPSAR"
     gene            976..2274
                     /locus_tag="VFQ10_05365"
     CDS             976..2274
                     /locus_tag="VFQ10_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET9964802.1"
                     /translation="MAEVTFDYQNLIEVEGGLRSGELDELGSRLREAAHELRGDPPGF
                     MRLPKTSEYAEASARAAEEIRTSGATDFIHVGIGGSALGPMALHRALSHPYYNTLSGE
                     ARRGPRLHFAENTDPATLSAILDVADPEGTWVNVVTKSGSTAETMANFLVIRGFLVDN
                     LGDFGFQERTIATTDSEEGFLKQISDREEFKLLPIPPDVGGRFSVLTPVGLLPAAVIG
                     LDVDALLAGAAQCVDEVNEGGAEHPAVVGAAMHYLMDTARGRNVRVMMTYADALERLA
                     AWFVQLWAESLGKDGKGSTPHGAVGTTDQHSQVQLYMQGPQDKVIEIVEVENHPRDLR
                     IPGAYEDLEGVGYLGGHTMAELLNVECDATRRALTEAGRPNCTIRLGEPSEENLGYLL
                     QALEVQTAIAGALYGVNAFDQPGVEAGKQITYKRMGRPGY"
     gene            complement(2356..3168)
                     /locus_tag="VFQ10_05370"
     CDS             complement(2356..3168)
                     /locus_tag="VFQ10_05370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9964803.1"
                     /translation="MRRVEPLINLALLLPGRPWEFRDRVAGYADLGLEQLWRRSPDYE
                     TASWQGVLGDIEAHLGRVTEILDEPALEEVEEDTRRLLEEIRARDPFSPRWAADSRFA
                     RLGYLLCRLISPSVVLETGVAYGVSSVFILKALEVNGRGTLHSIDLPPLRSEHERFWG
                     IAVPEPLKARWRLHRGSSARVLPRLLEEASTIDLFVHDSLHTLRNMRREFDTVWPHLR
                     NGAVLMADDVERNRAFSELRRKSPTLWRVVQDREESPLHGRAAPVVFGIAIK"
     gene            3260..3877
                     /locus_tag="VFQ10_05375"
     CDS             3260..3877
                     /locus_tag="VFQ10_05375"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HET9964804.1"
                     /translation="MKWLKVYGLPKLPLFLARWAMKLGLPGPTWYKWKAASAGTGVIL
                     DEMIRAADDLGYDGQKVGKLAMSRIGEKQGSDLREQLGVKTMKDAVDVVMLANRFFDI
                     EITLTEQGDGTYVINADRCPWFGGMGQEGVPGWDVKPCAAFSTYEKAMVQAINPNVKL
                     SYTEKRTTGGHTCRGVYQYRDEELGDGPAEGVQPEMVSIGKKPKV"
     gene            complement(3909..4856)
                     /locus_tag="VFQ10_05380"
     CDS             complement(3909..4856)
                     /locus_tag="VFQ10_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9964805.1"
                     /translation="MKGGGEARPVKVLYVAGLGRSGSTILANTLGQVEGFFSGGELNF
                     IWKHTLIENRLCGCGKPSQECEFWGPVFDGEFGGQSEELAREMMRLQYSGARTRHIPM
                     MLTESGRQKIRARLGKFLDNTGRLYRAIQSVSDSRVVVDTSKEPAYGYALGMVPGLDL
                     RVLHLIRDPRAAAYSWTKKKRQPDSTEREFMHQKSPTQSAVLWDAWNAAIEALWRQMP
                     ARYLRLRYEDFVADPRRSFEEILELTGEEDAQLPLVGERDVKLGISHTVSGNPNRFDT
                     GTVELRQDRAWQKQMKPRDRALVTALTLPLLTRYHYSVR"
     gene            complement(4853..6529)
                     /locus_tag="VFQ10_05385"
     CDS             complement(4853..6529)
                     /locus_tag="VFQ10_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HET9964806.1"
                     /translation="MTSRRDFEKEHNRGREEKVSEGEPTAEQVDEPIGPKDDDTYVAK
                     VARGAGISTAGQGVGRVIGYVTQVAIARLLGAQFYGFYTAGVALINGLQILSRFGMEN
                     GVVRYVAHYREHQDASRVRGTIVMALLFPIALSVVLSAVLFFGAGFLADSGWLKENGA
                     MMVGVLRGFAFVLPFFVFMSMTAWATQGFQTVTYAAYIQQLVRPGLFLVFVGVCYVLG
                     AKIFGVIAAYALAMFLAGLVGLYYLRKLFPQLFDRRAHTAFETKALFGVSVPMSITQG
                     AQYLNNFSAILILGIFAAGAPVGIFNAAARTATFLTAVRFAFSGIFSPIISGLYARQN
                     MEEMGRLYKDVSRWIFTGAFVLFLVIVVFGPEVMGIFGEAFGAGVTALVIVAVAQLYS
                     SSVGPAPRMLAMTENQNFAMIATSVAAITGVVVSFILIPRFEVLGAAIGMATAIIVEN
                     TGTMSAVKWRLGYWPFNIMWLKPLTAGAISATATYLLKITLPLEAMFGFLPVPISVPT
                     IMVLGGFLGVLFLGLLWLFGFSETDKEFLGTFWNVAQRALPRRFKKGDQE"
     gene            complement(6587..7921)
                     /locus_tag="VFQ10_05390"
     CDS             complement(6587..7921)
                     /locus_tag="VFQ10_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET9964807.1"
                     /translation="MNVILVILDSLRKDHIGVYGNNKIKTPNLDALAEESLRFSRAYP
                     ESLPTLCARRAIHTGRRTWPFRNFVRLPGETFQPAGWQPIPEQQTTLSESLFASGYST
                     TLFADTQPLFHPSMNFQRGFSVFEWLRGQERDRFRPKLGVPEEQIRQRTVPGNDANMV
                     DKVRQHLANTQDRKTEEDWFAPRLFTRGMDFLEQAEDGQPFFLVIDSFDPHEPWDPPE
                     EYTESYSDGYQGRDPTVPNYGASDWIDDRELERMKALYSGEVTMVDRWFGRFLDKMES
                     TGRMEDTLLLVLADHGVALGEHGYTGKPYGALWPELTDIPFFIRHPEGHGAGQTSDYY
                     ASTHDVAPTILGALGLQPLEQMDGEDLNVLLEGSEPRPRPHFTLGYNEYVWARDDRYG
                     MFSVNDRSDPRLYDLRSDPDMDKNIAADNPDIVKRMFNKYILEDAGGPLPRF"
     gene            complement(8219..9601)
                     /locus_tag="VFQ10_05395"
     CDS             complement(8219..9601)
                     /locus_tag="VFQ10_05395"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9964808.1"
                     /translation="MRIVRDILKRLLGFKLLRRFTSALILMLADGLALAIGLGVAAWG
                     GKSDPAIGLAPLLLAAWITLFSAFRLYDKAPVRRSPGGLLGAAFCWAGLVTIGATIYP
                     EIGLGLGEILSTAVLGLLGSSALRFLYERAIGRIYRRGFGQTPVLLVGDEEGRRRLRR
                     MMEHVPGAYALAGEVDLGSGGSAYLASVREVLDRTEARGVVLVGAEGLPDEELLDLVH
                     SVRLRGVPLRVVPGAFALMRGRTILSEGMGLPLLEVHYPGLDNTQRTLKRVLDVTVSL
                     GGLVLFSPLFLAVAIAVRLDSPGPVFFRQKRVGADEKVFVCYMFRSMQRDAEVRQADF
                     EDLNEVEGPAFKIREDPRVTRVGRFLRRWSVDELPQLANVLKGEMSLVGPRPLPVRDF
                     LGMEESHKGRLGAVPGMTGYWQTSGRSDLSFEEMVRLDLYYIENWSLSFDLKIILKTL
                     GAVIRREGAY"
     gene            9700..10461
                     /locus_tag="VFQ10_05400"
     CDS             9700..10461
                     /locus_tag="VFQ10_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964809.1"
                     /translation="MRTIEERFGSYVDEHTAMFRFEKWLQIYAGKHPSAFYGLYRLAR
                     KDRARIVTPDTQLVIEGFPRSANTFARVAFNRAQSERVRIAHGLHVPAQVIRASRWRI
                     PTLVLIRKPKDAVLSFAIRDPISVDQALRYYLSFYETVEEYRDAYVLGLFEEVTEDFG
                     EVIRRINDRFGSAFSPFSHDERNVEGVFARIEKNSKKRFGETSLEDKVSRPFASREKL
                     KREVGYELEDQKRRDLISRAETVYDRLTHKPAQDH"
     gene            complement(10458..>11141)
                     /locus_tag="VFQ10_05405"
     CDS             complement(10458..>11141)
                     /locus_tag="VFQ10_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HET9964810.1"
                     /translation="NVNAHCLNLCYEDSALRDSLNGADVVFCDGAGVMLAARMLGGRI
                     PARITYAAWIWRLAGFAASEGFSLYFLGARPGVAQEAARRLKARYPGLRIVGVRHGFF
                     DHSAGSAENEVVLEEINAAAPDILLVGLGMPLQEIWLTQNRHRLDARVALTGGAVFDY
                     VSGRSSRGPRILTERGFEWLARLLHEPRRLWRRYLLGNPLFLLRVLRQLLWRTTGRGL
                     VKSPPQDRR"
BASE COUNT         2178 a         3465 c         3475 g         2023 t
ORIGIN      
        1 agttcctgga gcacatcttt agggattctg atcctctctt cttgctcgct cactcgccga
       61 agcacctcct ccaccagggc atctgtacgc cctcctgagc cccttcgccg gcgggccggg
      121 gcttcgtata ctggtttctc ctctcctaca aagagtagtg tgtgctcgcc ttggtaggtg
      181 cccgcgccgc gcctcctttt tctaagctat ggccaattct aaccgctcgg tgccgccttc
      241 cgtcagcagt gcacggaact caatgttttg tactaccgag ccgacgggca gatatcgttt
      301 agaatgcagt ttgggcagtc tggcttgcgg gccttgcacg ttcgacgccc gtgcaagatc
      361 aggaggtggg agaagatggt ccggtcttct tctggtacta cctccatgag ctcctgctcg
      421 atgttcacgg gatcactact ctcggtgagg ccgagcctgc ccgagacacg gcggacgtgc
      481 gtgtccacga cgatgccctc gttcgtaccg aaagcgttgc caaggaccac gttggccgtc
      541 ttgcggccga cgcccggcag ggctacgagc tcggacatgg tgcgggggac ctctcccgcg
      601 tgcttctcta cgagggcgtt ggccatgccc cgcaatgagc gggccttgtt gcggtagaag
      661 cctgtcgggc ggatgtcctc ctcgagctcc tcggggttag aatcagcgta gtcctcagcc
      721 gtacggtact tcgcgaagag gctctcggtc accctgttga cctgcatgtc ggtggtctgg
      781 gcggagagga tggtcgcgat caggagttcc aaggggttgg accaatcgag ctcggtgcgc
      841 gcgtccgggt actcggcctt caagcgggct gttacttctg aggcgggtgc tgcggatagg
      901 ctcacgatgg ccggggatta taccctgttc taaactgtcc ccgtaagaga gaccgcgacc
      961 gaggggagcc tcagcatggc cgaagtcacc ttcgactacc agaacctcat tgaggtggag
     1021 ggcggtctgc ggagcgggga gctggatgag ctaggatccc gtttacgtga ggccgcgcac
     1081 gagcttcgcg gggacccgcc aggctttatg cggttgccga agaccagtga gtacgccgag
     1141 gcatcggcgc gcgcggcgga ggaaatccgg acctcagggg cgacggattt tatccacgtc
     1201 ggcatcggag gttcggccct cggtccgatg gctttgcaca gggcgttgag ccatccctac
     1261 tacaacacgc tctcgggcga agcccggcga ggtccgcgct tgcacttcgc ggagaacacc
     1321 gatcctgcga cgctctccgc catcctcgat gtcgctgatc ccgaaggaac ctgggtcaac
     1381 gtcgtcacca agtcagggtc gacggcggag accatggcga acttcctcgt gatccggggc
     1441 ttcctagtag acaatttggg agacttcgga ttccaggaac gcacgatcgc cacgacggac
     1501 tccgaagaag ggttcttgaa gcagatatcc gacagggaag agttcaaatt gctccccata
     1561 ccgcccgacg tgggcggccg gttctcggtc ctcacacccg tagggctgtt gcccgccgcc
     1621 gtgatcggtc tcgacgtgga cgcgctgctc gccggagcgg ctcagtgcgt tgacgaagta
     1681 aacgaaggtg gcgccgagca tccagcggtc gtaggggctg ccatgcacta cctcatggat
     1741 accgcgaggg ggcgcaacgt cagggtaatg atgacctacg ccgacgccct ggagcggctg
     1801 gcagcctggt tcgtccagct ctgggccgaa tccctgggta aagacggcaa aggctcgacg
     1861 ccgcacggcg cggtcggcac cacagatcag cactcgcagg tccagctcta catgcagggt
     1921 ccgcaggaca aggtgatcga gatcgtcgag gtcgagaacc acccgcgcga cctacggata
     1981 ccaggagcct acgaggacct cgaaggtgta ggctacctcg ggggccacac catggcagag
     2041 ctcctgaacg tcgagtgcga cgccacaagg cgggccctca ccgaagccgg acgccccaat
     2101 tgcacaataa gactcggcga gccaagcgaa gaaaacctcg gctacctgct acaggcgctc
     2161 gaagtgcaga ccgccatcgc cggagctctc tatggcgtca acgccttcga ccagcccggc
     2221 gtcgaggccg gaaagcagat cacgtacaag aggatgggga ggccagggta ttagcatttc
     2281 agcaagtgtg cgggcgaaag ctgagatgct gacaagcgga gcccgaaggg cgtagcgcgc
     2341 tgacttgctg accagctact tgatcgcgat cccgaacacc acgggtgcgg ccctgccgtg
     2401 gagcggcgac tcttccctgt cctggaccac gcgccagagt gttggacttt tccgccgcag
     2461 ttcgctgaat gcgcggttcc tctcgacgtc atcggccata agtacggctc cgttgcgaag
     2521 atggggccat acggtatcga actcgcggcg catgttcctg agggtgtgca ggctgtcgtg
     2581 tacgaatagg tctatcgtgc tggcctcttc gaggagtctg ggcagaaccc tggcgctgga
     2641 cccccgatgt agtctccagc gggctttgag cggctcgggc acggctatgc cccagaacct
     2701 ctcgtgctca ctcctcaggg gaggcagatc tatgctgtgg agtgtgccgc ggccgttcac
     2761 ctcgagggct ttcaggataa agaccgagct cacaccgtag gccacaccgg tctccagcac
     2821 tacggaaggc gagatcagcc ggcacaaaag ataaccaagg cgggcgaacc tggagtccgc
     2881 agcccaacgc gggctgaagg gatccctagc gcggatttcc tccaggagcc ggcgggtgtc
     2941 ttcttctacc tcctcgagag ccggctcgtc gaggatctcg gtcacgcgtc ccaggtgcgc
     3001 ctcgatatct ccgagcacac cctgccaact tgcggtctcg tagtccggtg accttctcca
     3061 gagctgttcc aggccgaggt cagcataacc tgccaccctg tcgcgaaact cccagggacg
     3121 cccagggagc aggagagcaa ggtttatcag cggttcgacc ctacgcacgg ctcacggaca
     3181 ctccttcggt actccacgct cggaggtcag agtataatgg gccttgcgct ggcgtaagcg
     3241 aacgaagggt ttgcccatta tgaaatggct caaggtctac ggtcttccga agctcccact
     3301 attcttggcg cgatgggcga tgaagctcgg gctgcctggg ccgacgtggt acaagtggaa
     3361 ggccgcgagc gcgggaactg gcgttatcct ggacgagatg atccgggccg ccgacgacct
     3421 gggctacgac ggccagaagg tgggcaagct cgccatgagc cgcattggtg agaagcaggg
     3481 tagcgatctg cgcgagcagc tcggtgtcaa gacgatgaag gacgccgtcg acgtcgtcat
     3541 gctcgccaac cgctttttcg acatcgagat cacactgacc gaacagggcg acggaaccta
     3601 cgtcataaac gcggaccgct gcccgtggtt cggcgggatg ggacaggaag gcgtcccagg
     3661 ctgggacgtg aagccctgcg ccgctttctc cacctacgag aaggcgatgg tccaggccat
     3721 aaaccccaac gtaaagctct catacaccga gaagcggacc accggcggcc acacctgccg
     3781 cggcgtctac cagtaccgcg acgaggagct aggcgacgga cccgcggagg gcgtgcagcc
     3841 cgagatggtc agcatcggca agaagccgaa ggtgtaaaga gcttcgaggc gtcggtaggt
     3901 tgcgccggct accttaccga gtagtggtag cgtgtcagca gcggcaaggt cagcgctgta
     3961 acgagagccc tgtcacgggg tttcatctgt ttctgccacg cgcggtcctg gcgtagctcg
     4021 acggtacccg tatcgaagcg gttggggttg cctgagacgg tgtggcttat accgagcttg
     4081 acatcacgct ctcccaccag cggcaactgg gcatcttcct cacccgtaag ctccaggatc
     4141 tcctcgaagc tgcgacgagg gtcggcgaca aaatcctcgt agcgcaagcg aaggtagcgg
     4201 gctggcatct ggcgccagag ggcctcgatt gcggcgttcc aggcatccca gaggacggcg
     4261 ctctgcgtgg ggctcttctg gtgcatgaac tcgcgctcag tgctgtcggg ctgtcgcttc
     4321 ttcttcgtcc aggagtaggc cgcggccctg gggtctcgga tcaggtgcaa aacccgcagg
     4381 tccaggccgg gcaccatgcc gagcgcgtaa ccgtaggctg gctccttgga ggtgtcgacg
     4441 accacgcggc tgtccgagac tgactggatc gcccggtaca acctgccagt gttgtccagg
     4501 aacttgccga gacgggccct gatcttctgc ctcccgctct ccgttagcat catgggaatg
     4561 tggcgggtac gggcgccgga gtactgcaac cgcatcatct cgcgggccag ctcttcgctc
     4621 tggcctccga actctccgtc gaagaccggg ccccagaact cgcattcctg agacggcttg
     4681 ccacagccgc agagcctgtt ttcgatcagg gtgtgcttcc agatgaagtt cagctcgccc
     4741 ccggagaaaa agccctcgac ctgtcccagg gtgttggcca ggatcgtgct gccgctccgc
     4801 ccgagtcccg cgacgtagag cacctttacc ggccttgcct caccgccgcc cttcactctt
     4861 gatccccctt tttgaagcgc ctcggcaggg ctcgctgcgc cacgttccag aaagtgccga
     4921 ggaactcctt gtccgtctcg gagaagccga acaaccatag caatccgagg aacagcacgc
     4981 ccaggaagcc gccgagcacc attatcgtgg gaacggatat tggcaccggc aggaacccga
     5041 acatcgcttc cagcggcaag gtgatcttca acaagtacgt cgcggtggca gagatggctc
     5101 ccgctgtcag gggcttcagc cacataatgt tgaaaggcca gtatcccagc cgccacttca
     5161 cggcggacat cgttcccgtg ttctcgacta taatcgcggt agccataccg atggcggctc
     5221 ccagaacctc gaagcgcggg atgaggatga agctcacgac aacgcccgtg atggcggcga
     5281 cggaggttgc gatcatggcg aagttctggt tctcggtcat ggcgagcatt cgcggcgcgg
     5341 gtccgacgga agagctgtag agctgggcga cggcgacgat gacgagggcc gtaacgccag
     5401 ccccgaacgc ttccccgaaa atccccatca cctcgggacc gaagaccacg atcacgagga
     5461 agagcacgaa cgcgcccgtg aagatccagc gcgacacgtc tttgtagagc ctgcccatct
     5521 cttccatgtt ctgcctcgcg tacaggccgg agatgatcgg ggagaagatg ccggagaagg
     5581 cgaagcgcac cgcagtcagg aacgtggccg ttcgcgcggc ggcgttgaag atgccgactg
     5641 gagccccggc ggcgaagatg cccaggatca ggatggcgga gaagttgttg aggtactggg
     5701 cgccctgcgt aatgctcata gggacggaga cgccgaagag ggctttggtc tcgaaggcgg
     5761 tgtgcgcccg gcggtcgaag agctgcggga agagcttcct gaggtagtag agcccgacga
     5821 gtccggccag gaacattgcg agcgcgtagg cggctattac cccgaagatc ttggccccta
     5881 gcacatagca tactccgaca aagaccagga acagcccggg gcgcacgagt tgctggatgt
     5941 acgctgcgta cgtcacggtc tggaaaccct gggtggccca cgcggtcatg ctcatgaaca
     6001 cgaagaatgg aagcacgaac gcgaaccctc gcaacacgcc aaccatcatg gccccattct
     6061 ctttcagcca accggagtcg gcgagaaagc cggccccgaa gaacaacaca gcggagagaa
     6121 cgacgctcaa cgctatcggg aagagcaggg ccatcactat ggtgccgcgt acccgcgagg
     6181 catcctggtg ctctctgtaa tgggcgacgt aacgcaccac accgttctcc atgccgaacc
     6241 gggagaggat ctgtagaccg ttgatcagcg ctacacccgc ggtatagaaa ccgtagaact
     6301 gcgcgccgag caaacgggct atggcaacct gggtgacgta cccgataaca cgcccgacgc
     6361 cctgtccggc cgtgcttatt ccggcgccac gcgcgacctt ggcgacgtaa gtgtcgtcgt
     6421 ctttgggtcc gatcggctca tctacctgct ccgcggtagg ctcgccctcg gagaccttct
     6481 cctcgcgccc gcggttatgt tctttctcga agtctcgtct cgaagtcacg gcgacagatt
     6541 atagaggttt tgggcctgac gaaaaggtcc aggctcttgt gggggatcag aaccgaggga
     6601 gcggtccccc ggcgtcctcg aggatgtatt tattgaacat ccgcttcacg atgtcaggat
     6661 tgtctgcagc gatgttcttg tccatgtcgg gatcgctcct gaggtcgtag agccgtgggt
     6721 cggagcggtc gtttacgctg aacatgccgt acctatcatc gcgggcccac acatactcgt
     6781 tgtagccgag cgtaaagtga gggcgtggcc ggggctccga gccttcgagc aggacgttca
     6841 ggtcctcgcc atccatctgc tctaacggct ggaggccgag ggcgcctagt atcgttgggg
     6901 ccacgtcgtg ggtagaggcg tagtagtcgc tggtctgtcc tgctccgtgg ccctcaggat
     6961 gcctgataaa gaacggtatg tccgtaagct cgggccagag cgccccgtac ggcttgcccg
     7021 tgtagccgtg ctcgccgagg gctacaccgt gatcggccag aacgagaagc agggtgtctt
     7081 ccatgcggcc cgtggattcc atcttgtcca ggaagcggcc gaaccagcgg tcgaccatcg
     7141 tcacctctcc cgagtacagc gccttcatcc tctccagttc ccggtcgtcg atccagtccg
     7201 aggcgccgta gttggggacg gtgggatccc tgccctggta gccgtcgctg taggactcgg
     7261 tgtactcctc gggcggatcc cacggctcgt gtgggtcgaa gctgtctatg accaggaaga
     7321 aaggctgtcc atcttcggcc tgctccagaa agtccatccc tctggtgaag agccgcgggg
     7381 cgaaccagtc ctcctcggtc ttgcgatcct gcgtgttggc caggtgctgg cgcaccttgt
     7441 ccaccatgtt ggcgtcgttt ccggggaccg tcctctgcct tatctgctcc tcggggacgc
     7501 ccagtttggg gcgaaagcgg tccctctcct ggccacggag ccactcgaac accgaaaaac
     7561 cacgctggaa gttcatcgag gggtgaaaca gaggctgggt atcggcgaac aaagtggtgc
     7621 tgtagcctga tgcaaacagg ctctcggaga gcgtcgtctg ctgctccggg atcggctgcc
     7681 agcctgccgg ttggaaggtc tctcccggca acctgacgaa gttcctgaaa ggccaagtcc
     7741 tcctgccagt gtggatcgcc ctgcgggcgc aaagcgtcgg caacgactcg gggtaagccc
     7801 tactgaagcg caggctttcc tctgccagcg catccaggtt gggggtcttt atcttgttgt
     7861 tgccgtagac cccgatgtga tctttcctga gactgtccag gatcaccagt atgacgttca
     7921 cttctcgtac ccccgtatgg ctaagtagcc gtaagctcgg gacgccgcta ttctagatca
     7981 atagacctcc ttttatcagc atgtcagcac gcgccctgtg gggcgcttgt cagcccgcct
     8041 ccctccggtc ggcggctctc agcaattcag caaagtcctc ccgcagcctc cccacaagcg
     8101 tcgcacccga tctccgcctc aaccgtccct agtgcgctgc agggcaaggc cgaaaccaat
     8161 aagctgacct gctgacaagc gagggcctgc aaggcctgaa gcgtgctgac ttgctgagtc
     8221 agtacgcacc ctcgcggcgt ataacggcgc ccagggtctt caggatgatc ttgaggtcga
     8281 atgacagcga ccagttctct atgtagtaga gatccagacg caccatctcc tcgaaggaga
     8341 ggtcggagcg gccgctagtt tgccagtatc cggtcatccc aggaacagca cctagcctcc
     8401 ctttgtggga ctcctccatc cccaggaaat cacgtaccgg caggggcctc gggccgacca
     8461 ggctcatctc ccctttgagg acatttgcga gttggggcaa ttcgtctacg ctccagcggc
     8521 gcaagaaacg cccgaccctg gtcacgcggg gatcttccct gatcttgaag gccggtccct
     8581 ctacctcgtt gagatcctcg aagtcagcct ggcgtacttc ggcgtccctt tgcatggagc
     8641 gaaacatgta gcagacgaag accttctcgt cagccccgac gcgcttctgc cggaagaaca
     8701 cgggtcctgg cgagtcgagc ctgaccgcga tggccacagc cagaaagagg ggcgaaaaga
     8761 gcacgagccc acccaaggag accgtcacat ctagaacacg cttgagggta cgctgggtat
     8821 tgtccagccc cgggtagtgg acctccagga gcggcagccc catgccttcc gagagtatgg
     8881 tacggccccg catcagcgcg aaagcgccgg gcaccaccct gagcggcacc ccgcgcagtc
     8941 gcaccgagtg gacgagatcg aggagctcct cgtcgggcag accctcagcc ccgacgagga
     9001 cgacgccacg ggcctcagtg cggtccaaca cttcccttac cgacgcgaga tacgcgctgc
     9061 ccccgcttcc gaggtcgacc tcaccggcca gggcgtaggc tccgggcacg tgctccatca
     9121 tccggcgcaa ccgcctcctg ccttcttcat ctccgaccag aagaaccgga gtctgtccga
     9181 aaccgcgacg gtagatccta cctatggcgc gttcgtagag gaacctgagc gcgctggagc
     9241 ccagcaagcc gagcacggct gtcgacagta tctccccgag ccccaggccg atctcggggt
     9301 agatagtcgc ccctattgtc accaacccgg cccagcagaa cgcggccccg aggagtcctc
     9361 cagggctgcg ccgtacgggc gctttgtcgt agaggcggaa agcgctaaag agcgtgatcc
     9421 aggccgcgag caacagcggc gcgaggccga tggcaggatc cgacttgccg ccccaggcgg
     9481 ctactcccag cccgatagca agagcgagcc cgtccgcgag catcaggatg agcgcgctcg
     9541 taaagcgccg cagtagcttg aagccaagga gtctcttcag gatgtctctc acgatccgca
     9601 cggggtcctc cgtacgagga gtataccgcc gcgcaatgcc aaccagctcc cgcagagtgt
     9661 gtaagattga gggtgagaca gtaggcggag agtacaggag tgagaaccat cgaggagcgg
     9721 ttcgggtcct acgtggacga acacaccgcc atgttcaggt tcgagaagtg gctccagatc
     9781 tacgcgggca aacacccttc agccttctac ggtttgtacc gcctggcgcg caaggaccgg
     9841 gcgcgcatcg tgaccccgga cacccaactg gtcatagagg gcttcccgag gtcggccaac
     9901 acgtttgcca gggtggcctt caacagggcg caaagcgagc gggtgcgcat cgcccacggt
     9961 cttcacgttc cggcgcaggt gatccgggcc tcacggtggc ggatacctac ccttgtgctg
    10021 atacgaaaac ccaaagacgc cgtgctgtct tttgccatac gagacccgat ctccgtggac
    10081 caggccctca ggtactacct ctccttctac gagaccgtcg aagagtaccg cgatgcctac
    10141 gtcctcgggc tcttcgaaga ggttaccgaa gacttcggag aggtcatccg gcgcataaac
    10201 gacaggttcg gatccgcctt ctcaccgttc agtcacgacg aacggaacgt cgaaggtgtg
    10261 ttcgcgcgca tcgagaaaaa ctccaagaag aggttcgggg agacgtcctt ggaggacaag
    10321 gtctctcgcc ccttcgcctc cagagagaag ctcaaacgcg aagtgggtta cgaactggag
    10381 gaccagaaac gcagggactt gatctccagg gccgagaccg tttacgaccg cctgacacac
    10441 aagccggctc aggatcacta acggcgatcc tgcggagggc tctttacgag gcctcttcca
    10501 gttgtcctcc agagtagctg tctgaggacc cgcaatagga acagggggtt accgaggagg
    10561 tagcgtcgcc acaggcggcg gggctcgtgg aggagccgcg ccagccactc gaagcccctc
    10621 tcggtcagga tgcgcggccc gcgagacgac ctccctgaga cgtagtcgaa gacggcgccg
    10681 ccggtgaggg cgacgcgggc gtcgagccgg tgcctgttct gtgtcagcca gatctcctgg
    10741 agcggcatcc cgaggccgac aagcaggatg tcaggggcgg cggcgttgat ctcctcgagg
    10801 acaacctcgt tctcggcact cccggccgag tggtcgaaga agccatgacg gacgccaacg
    10861 atcctcaagc cgggataacg tgccttcaac ctcctcgcgg cctcctgagc cacgccgggt
    10921 cgggcgccga ggaagtagag tgagaagccc tcggacgcgg cgaagccggc caggcgccag
    10981 atccaggctg cgtaggtgat gcgtgccggg atgcgcccgc caagcatcct cgccgccagc
    11041 atgaccccag ccccgtcaca gaagacgacg tcagcaccgt tcagcgagtc ccgtagtgca
    11101 gaatcttcat agcagaggtt gaggcagtgg gcgttcacgt t
//
LOCUS       DASTKM010000124         5845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1263683, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000124 DASTKM010000000
VERSION     DASTKM010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263683"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /gene="cimA"
                     /locus_tag="VFQ10_05410"
     CDS             complement(<1..1216)
                     /gene="cimA"
                     /locus_tag="VFQ10_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET9964811.1"
                     /translation="MSIKLFDTTLRDGTQREGVSLTAEDKARIARELDSLGIHYIEAG
                     FPASNPKDLEFFEGFDDSSLSHAKLVAFTRARRPKGRAEDDPAVTLLPSLSAPVACIV
                     AKSWKLHVEKVLRTTPEENLESVRDTVAYLAGAGKEVIFDAEHYFDGFRDDPEYSLAV
                     LRAAAEAGTTTLVLCDTNGGTLPTELKAVVARTVREFPDVEVGIHTHNDSGCGVANAL
                     VAVESGATHLQGTINGIGERCGNCDLVTTIANMQLKMGLKVMDDERLRRLTEVSNYVS
                     ELMNLTPDTHRPYVGRSAFAHKGGLHVDGISKDPATYEHVAPEAVGNVRRTPVGELSG
                     KNSIRTKARELGLEAKDATRILGAIKQREYEGYHYEAADASLALLIGRTIGEDRPLFE
                     LETFRIISEKRAG"
     gene            complement(1213..2280)
                     /gene="leuB"
                     /locus_tag="VFQ10_05415"
     CDS             complement(1213..2280)
                     /gene="leuB"
                     /locus_tag="VFQ10_05415"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET9964812.1"
                     /translation="MRKSFDVLLLPGDGIGPEVVGAARQVVDAAAEHFGIEVRYEERR
                     IGGTAIREEGEPVSTETLEAAGASDSVLLGAVGHPDYEDAPLRPEAGLLKLRKSLGAF
                     ANLRPVAAIPALIGSSPLKREVVDGVDVLIVRELTGGAYFGEKEEGEERASDLSVYTR
                     EEIERVARVAFDAAKRRKRRVTSVDKANVMATSRLWRRVVTEVAEDYPDVELDHVLVD
                     AAAMFLVQAPKRFDVVLTENLFGDILSDEAAMLPGSMGMLPSASLGKPGSPGIFEPVH
                     GSAPDIAGEGKANPYATILSAAMMFRHTLGRPDVAEAIEQGVSVALEAHFSTPDLGGN
                     KTTEEVTKAVSKWVAAGEGVA"
     gene            complement(2277..2936)
                     /locus_tag="VFQ10_05420"
     CDS             complement(2277..2936)
                     /locus_tag="VFQ10_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964813.1"
                     /translation="MRGYLAQGYSTLGRILSAEPPVIVAYLVADEDWNDAPRDNARPY
                     PTGLPYFTRSVEPPALVLPEELSTVFRPRTAATLPLTVWHELAHAFLLGREVVRTPAW
                     LGEFVPQAASAAVARRAGLSLREHLSQIEREPGLTVRSFRGPAGAGEQMSFQNLLLLL
                     GAAALDEFGEGFLRRIFHALWEEDEIVDEGRAEEMLADALGPDGRQWVVSRPEFQEEP
                     L"
     gene            complement(3038..4579)
                     /locus_tag="VFQ10_05425"
     CDS             complement(3038..4579)
                     /locus_tag="VFQ10_05425"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET9964814.1"
                     /translation="MRRVEVFDTTLRDGEQSPGISLSVEEKVEIARQLARLKVDVIEA
                     GFPITSEGDFESVSRIASEVKGPRITGLARIHEQDIVRAWEAVQWAQRPRIHTFVGTS
                     DLHIEHQMRSNREDILRRAGEAVAFAKNFCENVEFSPMDATRTDIGYLSEIVAAAVES
                     GADVVNIADTVGYTTPVEFADFLKELRERVPALEERILSVHCHDDLGMAVANSLAGVE
                     VGAGQVEVAVNGIGERAGNASLEEVVMALATRSDYYGARVGVETRQLANTSRMVSNIT
                     GYEVPPNKAIVGRNAFLHESGIHQDGVLKNRRTFEIMTKEDIGLEGTNIFLGKHSGRH
                     ALKDALEELGYYVEGDVLKRAFTRFKEIADHKKTVTAADLEAIAADEVGSFEGKFVLE
                     SFRVVAATGRQSRAAVTISHAEQGTFEAEAEGEGPVDAVFRAIDAATNIKGRLTDFRI
                     DAVTGGKDALGEVRVSVEFEGGDYAGRGLSQDVVEAAARAYVRAVNVYTAGMVGAKWD
                     AQVAP"
     gene            complement(4764..5720)
                     /locus_tag="VFQ10_05430"
     CDS             complement(4764..5720)
                     /locus_tag="VFQ10_05430"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET9964815.1"
                     /translation="MSRKAFAASDAEWAYHGGELVKVGDIRLSPATHALNYGTGVFEG
                     IRAYWSEQRGTLQVLKMREHYQRFERSCRLLRIELPNTVEELCEVTLEILSRNAPRED
                     TYVRPLAYKAAESVGVNLKGETELSIFTVPMGNYVELTGLKVCVSSWRRTPDNAIPAR
                     AKCTGSYVNTALAVDEAHMAGYDDAIFLTQDGQVSEASAANIFLLRKGQLITPPVTAD
                     ILEGITRDAVMELAGKELGIPVIEREVGRTELYAADEVFLSGTGFQIAPVVEVDDRPV
                     GTGSIGPVAERLQELYFKAARGEWEEYADWTVAIEVAERAAG"
BASE COUNT          918 a         1977 c         1845 g         1105 t
ORIGIN      
        1 cgccggcccg cttctcggag atgatcctga acgtctccag ctcgaagagc ggcctgtcct
       61 ccccgatggt ccgccctatc agcagggcga gcgaggcgtc agcggcctcg tagtggtagc
      121 cctcgtactc gcgctgcttt atggcgccga ggatccgggt cgcatctttg gcctcgagcc
      181 ccagctccct tgccttggtc ctgatggagt tcttccccga gagctcccca accggcgtgc
      241 gccttacgtt ccctacggcc tcgggcgcga cgtgctcgta ggtcgcaggg tccttggaga
      301 taccgtcgac gtgcagcccg cccttgtggg cgaaggcgct gcgccccacg tagggacgat
      361 gggtgtcagg ggtcaggttc atgagctccg agacgtagtt ggagacctcg gtgagccgcc
      421 gcaacctctc atcatccatg actttcagcc ccatcttgag ctgcatgttg gcgatggtcg
      481 tgacgaggtc gcagttgccg caccgctccc cgataccgtt gatggtcccc tgtaggtgcg
      541 tcgccccgga ctctaccgcc accagagcat tcgccacccc acaccccgag tcgttgtggg
      601 tgtggatgcc tacctccacg tcagggaact cccgcaccgt gcgcgccacg accgctttga
      661 gctcggtcgg caatgtcccg ccgttcgtgt cacagaggac cagcgtcgtc gtccccgcct
      721 cggctgcggc gcggagtacc gcgagcgagt actctgggtc gtcccgaaag ccgtcgaagt
      781 agtgctcggc gtcgaaaatg acctctttac cggcgccggc caggtaggcg acggtgtcgc
      841 gcacggactc gaggttctcc tctggcgtgg tccgcagtac cttctccacg tgcagcttcc
      901 agctcttggc gacgatgcag gctacgggag cggagaggga gggtagcaga gttactgcag
      961 ggtcgtcctc ggcccgccct ttcgggcgcc tggcccgcgt gaaggcgacg agctttgcgt
     1021 gggagagcga agagtcatcg aagccctcga agaactccag gtccttgggg tttgaggcgg
     1081 ggaagcctgc ttcgatgtag tggatcccca gtgagtcgag ctcgcgggct atgcgggctt
     1141 tgtcctcggc ggtcaggcta acgccctctc gctgggtacc gtcgcgtagc gtcgtatcga
     1201 acagctttat gctcatgcca caccttctcc cgctgcgacc cacttgctca cggccttcgt
     1261 cacctcttct gtcgtcttgt ttccgccgag atccggggtc gagaaatggg cctccagtgc
     1321 gacggagacg ccctgctcga tggcctcggc cacgtcgggc cggcccaggg tatggcggaa
     1381 catcatcgcc gcggagagga tggtcgcgta ggggttggcc ttcccctctc ctgcgatatc
     1441 gggggccgag ccgtggacgg gctcgaagat accgggggag cctggcttgc ctaaagacgc
     1501 gcttggaagc atccccatcg agcctgggag catcgcagcc tcatcagaga ggatgtcccc
     1561 gaagaggttc tcggtcagca cgacgtcgaa cctcttcggt gcctgaacga ggaacatcgc
     1621 cgccgcgtcg acgaggacgt ggtcgagctc cacatcagga taatcctcgg ccacctccgt
     1681 gacgaccctg cgccacagcc ggctcgtcgc catgacgttc gccttgtcca cgctcgtcac
     1741 ccgccgcttg cggcgtttgg ccgcatcgaa ggccacccgc gcgacccgct ctatctcctc
     1801 ccgcgtgtac acgctgaggt cgctggccct ctcctctccc tcctccttct ccccgaagta
     1861 agccccgcct gtcagctccc tgacgatgag cacgtcgacg ccgtcgacga cctcacgctt
     1921 gaggggcgag gagccgatga gcgcaggtat ggcggccacg ggcctaaggt tggcgaaggc
     1981 cccgaggctc ttccttagct tcagcagccc agcctccggg cgtaagggag cgtcctcgta
     2041 gtcggggtgc cccacggctc cgagcaggac cgagtcagag gcgccggcgg cctccagcgt
     2101 ctccgtggag acgggttcgc cttcctcgcg gatggccgta ccgccgatcc tgcgttcctc
     2161 gtagcgcacc tcgatgccga agtgctcagc cgctgcgtcg acgacctggc gcgcggcgcc
     2221 gactacctcg ggtccgatgc cgtcgccagg gagaagtagt acgtcgaaag actttctcat
     2281 aacggctcct cctggaactc tggcctcgac actacccatt gccggccgtc aggacccagg
     2341 gcgtctgcga gcatctcctc ggcccgcccc tcgtcgacta tctcgtcttc ttcccagagc
     2401 gcgtggaaga tgcgtcgtag gaaaccttct ccgaactcat cgagcgccgc cgcgccgagc
     2461 aggagcagca ggttctggaa agacatctgc tcccccgctc ccgccggtcc gcgaaagctc
     2521 cgcacggtca accctggctc cctttctatc tgcgacagat gctcccgcag ggacagaccg
     2581 gccctgcggg ctaccgccgc cgaggcggcc tgcggcacga actccccgag ccaagcaggg
     2641 gtccttacga cctcccgtcc gagcaggaag gcgtgggcca gctcgtgcca gacggtcagg
     2701 ggcagcgtgg ccgcggtgcg cggcctgaac actgtggata gctcttccgg cagcacaagg
     2761 gcgggaggct cgaccgagcg ggtgaagtag gggagtcccg tcggatatgg ccgcgcgttg
     2821 tcgcgcggcg catcgttcca gtcctcgtcg gccacgaggt aggcgacgat cactggaggc
     2881 tctgcggaca gtatccgccc gagcgtactg tagccctgcg cgaggtagcc tcgcacctca
     2941 cttgccttcc cggccagccg tggagcgtag aggaggggca ccttcccgtc caggcgttcg
     3001 taacccggcg gagcggcttc ccgtctgcgc ggggaagcta cggcgctacc tgcgcgtccc
     3061 acttggctcc gaccataccg gccgtgtaga cgttgacggc ccgcacgtag gcgcgcgccg
     3121 ccgcctcgac gacgtcctgg gagaggccgc gtccggcgta atctcccccc tcgaactcca
     3181 cagagaccct gacctcgccg agggcgtcct ttccccccgt cacggcgtcg atacggaaat
     3241 ccgtcagcct gccctttatg ttcgttgcgg cgtcgattgc gcggaagacc gcgtcgacgg
     3301 gcccctcacc ttctgcttcg gcctcgaacg tgccctgttc ggcgtgggag atggtgacgg
     3361 cggccctgct ctggcgcccc gtggcggcga cgaccctgaa agactcgagg acgaacttgc
     3421 cttcgaagga gccgacctcg tccgcggcga tagcctccag gtccgcggct gtcacggtct
     3481 tcttgtggtc cgctatctcc ttgaaacgcg tgaaggcccg cttgagcaca tcgccctcca
     3541 cgtagtagcc gagctcttcc agggcgtcct tgagggcgtg gcggcccgag tgcttgccga
     3601 ggaagatgtt cgttccttcc aggccgatgt cttccttggt catgatctcg aaggtgcgcc
     3661 tgttcttgag cacgccatcc tggtggatgc ccgactcgtg cagaaaggcg ttgcgcccca
     3721 cgatggcctt gttcgggggg acctcgtagc ccgtgatgtt ggagaccatg cggctcgtgt
     3781 tggcgagctg gcgggtctcg acaccgaccc ttgcgccgta gtagtcagag cgcgtggcga
     3841 gcgccatcac gacctcctcc agcgaggcat tccctgcccg ctcaccgatg ccgttgacgg
     3901 ctacctcgac ctggccggcc ccgacctcga cccctgccag cgagttggcg acggccatcc
     3961 cgaggtcgtc gtggcagtga accgagagga tgcgctcctc gagtgctggc acccgctccc
     4021 gaagctcctt caggaaatcc gcgaactcga ccggcgtcgt gtagcctacc gtgtcggcga
     4081 tgttgaccac gtcggcccct gactcgacgg ccgcggcgac gatctcagag aggtagccta
     4141 tgtccgtgcg cgtcgcgtcc atcggggaga actcgacgtt ctcgcagaag ttcttggcga
     4201 aggccaccgc ctcgccggcc ctccgcagga tgtcttcccg gttggagcgc atctggtgct
     4261 ctatatgcag gtcgctggtg cctacgaaag tgtggatgcg tggcctctgt gcccactgca
     4321 cagcctccca ggcacgcacg atgtcctgct cgtggatgcg ggcgaggcct gtgatcctcg
     4381 gccccttgac ctccgaggcg atgcggctca ccgactcgaa gtccccttcg gaagtgatgg
     4441 ggaacccggc ctctattacg tccaccttca ggcgggcgag ctggcgggct atctctacct
     4501 tctcctctac ggacagggag atgccaggcg actgctcgcc gtcgcgaagg gtggtgtcga
     4561 agacctctac tcgacgcatg agtctcatcc tcctacgttt ggaaaacctg cttgagaaat
     4621 gcgacttgag actggtggcc gcggacagcc ggctagatac cggggctggt aattcgcccg
     4681 gcggccaccc tggttagtcg taggatgcgg cgagaaacac gcaagatgtc tagaagttct
     4741 ccttcatgaa gggtggcagt atctcatccc gccgcccgct ccgcaacctc gatggcgacg
     4801 gtccagtcag cgtactcttc ccactcgccg cgggcggcct tgaagtacag ctcctgcagg
     4861 cgctcggcga cgggtcctat actgccagtc ccgacgggac ggtcgtccac ctcgacgacg
     4921 ggtgctatct ggaaccccgt ccctgagagg aagacctcgt ctgcggcgta gagttccgta
     4981 cggcctacct cgcgctctat gacggggatc cctaactcct tgccggccaa ctccatgacg
     5041 gcgtcgcgcg taatgccctc caggatgtcc gccgtgacgg gcggggtgat gagttggccc
     5101 ttcctgagaa ggaagatgtt cgccgcgctg gcctcggaga cctggccgtc ctgggtgagg
     5161 aagatggcgt cgtcgtagcc tgccatgtgc gcctcatcga cggcgagcgc ggtgttgacg
     5221 taggagcccg tgcacttggc gcgggccggg atggcgttgt caggcgtgcg ccgccaggaa
     5281 gagacacaga ccttcagacc cgtaagctcg acgtagttgc ccatggggac cgtgaagata
     5341 gatagttcgg tctcaccctt caggttcacc ccgacggact cggcagcctt gtaagccagg
     5401 ggacggacgt aagtatcctc gcgcggggca ttgcgactga ggatctccag cgtgacttcg
     5461 cagagctcct cgaccgtatt gggcaactct atacgtagca gtcgacagga cctctcgaag
     5521 cgctggtagt gctcgcgcat cttcaggacc tgcaacgtcc cgcgctgctc gctccagtat
     5581 gcccttatgc cctcgaagac accggtgccg tagttcaggg cgtgcgtcgc cggtgagagg
     5641 cgaatgtcgc cgactttcac cagctccccg ccgtggtagg cccattcggc gtctgacgcc
     5701 gcgaaagctt tacgactcat aggttctcac ctccagcgga gccagcgggc gtctcgatac
     5761 cctcggccgc gagcgcccgc agctcctgtc cgaccttctc cacctgcgac tcggcctccc
     5821 tgcggcgcat cgcgaggaag ctgga
//
LOCUS       DASTKM010000125        13897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_206355, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000125 DASTKM010000000
VERSION     DASTKM010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13897
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206355"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VFQ10_05435"
     CDS             complement(<1..964)
                     /locus_tag="VFQ10_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964816.1"
                     /translation="MERKKPVESWRNRKTLILSRTDMMGLLTPDEYNACVEHAYRMHG
                     EGRYYMEPKGHIVLDKYPGEWEVMPSYIEEPEAAACKWVSIREENRARFDLPTVFSIL
                     IYTHPETGFPLAICDGSYHTVMRTGAAAAVSTKHLARQDSKVLAIVGAGNMAEGTLAT
                     CNEVFRWEDVHVWSRSQTTLDDFVQSQQPKYDHFEIWASTNLEEIVRGADVVVTVTPA
                     RGPIVENGWISEGTHIAAVGADKEGDQELDPTILERARIFVDDIRQCRTDGEINVPFR
                     EGVISEDDIAGEIGQVVAGKLEGRTSDSDITVFDSTGFALQDSAT"
     gene            complement(1004..1450)
                     /locus_tag="VFQ10_05440"
     CDS             complement(1004..1450)
                     /locus_tag="VFQ10_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9964817.1"
                     /translation="MAESTPFREILEELLRSTGASRTTLRLDLLDQDAGLDEVVAEAL
                     APGVHSIKGDTSIRNLRDSSPIRFLEEQRTPLVQEDCSTADPAPPPELIELYGVRAQM
                     VAPLVRDDGLIGVISVHYVPGPRAWSAQDLAALQEATERVLRELDI"
     gene            complement(1455..2108)
                     /locus_tag="VFQ10_05445"
     CDS             complement(1455..2108)
                     /locus_tag="VFQ10_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET9964818.1"
                     /translation="MSSEQTREVYQRARLGQSVTLGARPAVLVVDFSRGFTDPECAMG
                     ADLTKEVEATRGLLDVAREAELPVIFTTIGFEENLKDGSLWLEKAPGLAELQVGGKWV
                     EIDPRLGRREEETVILKKGASAFFGTNLPSILVSQHVDTVIMCGATTSGCIRATAIDL
                     LQYGYPTLVPRECVGDRAQEPHEANLFDIQAKYADVVSAEEASAYIETVARKSGASA"
     gene            complement(2113..3528)
                     /gene="hydA"
                     /locus_tag="VFQ10_05450"
     CDS             complement(2113..3528)
                     /gene="hydA"
                     /locus_tag="VFQ10_05450"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HET9964819.1"
                     /translation="MSVLIKGGRIVTVADDYVGDIFIEDERVSLIGESLDMQADKVID
                     ASGKYVIPGAIDPHTHMEMPFGGTISCDDFTSGTISAAFGGTTSHVDFCLQEPGQSLP
                     DALATWHEKIERAKPVIDVGFHLAVTDLEEGGTLEDLRKAPDEGVTSYKLFMAYKGAI
                     MVDDETLFKTMQVASETGALVMVHAENGDAIDVLVKQALAEGKTEPKWHAATRPPITE
                     GEATNRAIQLARIAGSPLYVVHVSCKEAIDPIAQARTNDWHAWGETCTQYLFIDETAL
                     DQPDFEGAKYVYTPPPRPKENQEHLWHALATDVLSVVSTDHCPFNWDGQKTLGRDDFS
                     KIPNGGPGIENRLHMLHHFGVRQGRLSMNRFVELVSTNPAKFFGMYPRKGTIAVGSDA
                     DIVVFDPEKRVKISAETHHSNIDYNLFEGTEVSGAPEVVLVRGQVIVEGDRLVGKPGA
                     GKFVRRARFGEELPGGPGRTG"
     gene            complement(3589..4854)
                     /locus_tag="VFQ10_05455"
     CDS             complement(3589..4854)
                     /locus_tag="VFQ10_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HET9964820.1"
                     /translation="MATQPMTIDRKKSAERIERDIETLAGPDYTRSSESIMRYAYTPE
                     YRKTLDYFIGELETLGFEHYEDPVGTLVARNRSAGEKVFGVGSHCDSNRNGGKYDGTM
                     GVVTALEVCRLNEELGLDLPLQLISFLEEEGSGFGQMLLGSRIIAQRVTEEELREKFC
                     AVDDGRSFWEHAEEAGYEPERWQDSIRMLDDLIGWIELHIEQARVLQDTQNRIGVVNA
                     IAGYVHADITVHGRGDHAGATPMDFRQDAGIVMAECMLELERLAKEAGKGTVGTVGEI
                     ELRPNLINAVPGRARFSLDIRGVDEDGFRGVARDIAAFAKEAAEKRGMSAGYAQRQTL
                     SATPLDGRVVAALEEAAEASGEPYMAMHSGAAHDTMSIADRIPSAMVFVPCKDGISHS
                     PEEDAEPADAALGAEIMLNAIRTFTADGQ"
     gene            complement(4878..5882)
                     /locus_tag="VFQ10_05460"
     CDS             complement(4878..5882)
                     /locus_tag="VFQ10_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET9964821.1"
                     /translation="MALPRVAVLSLGGTISSTDSGGKGVEPTLTGEALVSDVPEIAEV
                     AEVSAISFRQAASGELTVDDLVELASEIEDRIDEGVSGAVVTQGTDTIEETAFVLDLL
                     VDRDAPVVVTGAMRNPTLPGADGPANLLASIQVAASETARELGTVVVLNDEIHAARFV
                     RKTHTSNPATFRSDPVGPVGWISEDVPRVVVRPVGRHKITLPGDAQDRPVALHTFTLG
                     DDGRLLPEIERHGYAGLVVEALGGGHVPSITVDALEDLAVKMPVILASRTGGGEVLHS
                     TYGFIGSEMDLLERGLIYAGPLDGLKARLYLALLLRAGATREEIRNSFDSWLDVPVSS
                     "
     gene            6068..7018
                     /locus_tag="VFQ10_05465"
     CDS             6068..7018
                     /locus_tag="VFQ10_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HET9964822.1"
                     /translation="MAEKEIFCSIGIDIDAVAGWLGSYGGEDSPDDISRGMFSGEVGT
                     PRLLKLFEKYDLRTTWFIPGHSIETFPDQVKMVVDAGHEVGSHGYSHENPVAMTPQQE
                     RDVLEKCLELIENVAGRKPVGNVAPWWEVSATTNELLLEYDYKYDHSLQHRDFTPYYA
                     RVGDSWTKIDYSKAAEEWMKPLVRGEEIDLVKIGGNWYLDDLPPMMFIKGSPNSHGFV
                     NPHDIEELWRDEFDWVYREMDYATYCLTIHPDVCGRPQCLLMLERLIDYINGHDGIRW
                     TTMEEIADDFVERYPRSQPNHDPGINMYGVETPAAEGATS"
     gene            7031..7402
                     /locus_tag="VFQ10_05470"
     CDS             7031..7402
                     /locus_tag="VFQ10_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964823.1"
                     /translation="MCVLCGQDFVARVHWTDRHVEDRARAAVPGFDPVAFQGDRRRDR
                     AHRVVLTNEVLRYYGLRVDDWSGSKYVLRDRKGRSEIIQDLGSLWPAAAKLAGRTLDP
                     LDPVLREMLGGESALAAADSA"
     gene            7407..8435
                     /locus_tag="VFQ10_05475"
     CDS             7407..8435
                     /locus_tag="VFQ10_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9964824.1"
                     /translation="MEGKTTRPVTVVTGFLGSGKTTLLARVLSAPSMANTAVLVNEFG
                     EVGLDHHLLRRVDERTVLLGSGCVCCTTREDLVGALMELLDMDQRGEIPRLDRVVIET
                     TGLADPAPILHTIYADPVLQHHFSVDLVLTTVDAVNGALHLDRNPESVKQVAAADKVV
                     VTKTDIAEPGIVPGLLSQLGKINPSAWTCEAAFGEVDVHELFRPDGAGTHAAPAAFTA
                     ESGDPHDVGDTHSVSVTFDGAIDWTAFGIWFSMLLNARGEDVLRVKGLLDVGEAGPVV
                     INGVQHIIHPPEHLGEWPDEDRRSRIIFITRYIHPEELLDSLLAFRGLLGARPLPLHA
                     AEQRVPFP"
     gene            complement(8502..8948)
                     /locus_tag="VFQ10_05480"
     CDS             complement(8502..8948)
                     /locus_tag="VFQ10_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964825.1"
                     /translation="MIGRRVGDPPRTSEHILRMDPTLDLERRRLLISAALTAQARRHL
                     GSSLAANVSVGELLDLACGVSEGAGTIERAYRLRFDPPYPGVTAGPQSVGGTSRLMLA
                     CSVRDRVGTPIGVVFTTLIAGRPPQVSVAPAGTSIPEGWRSPGDFH"
     gene            8996..10933
                     /locus_tag="VFQ10_05485"
     CDS             8996..10933
                     /locus_tag="VFQ10_05485"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET9964826.1"
                     /translation="MSEPRNLTRKLLGDHLLEGDLVPGEEMDLTVDQILIEDATGSMT
                     ALQFEALGADSVAVPLAVMYVDHNVLQIDDKNMDEHRFLQSFSSHYGIQYSRPGNGIS
                     HYVHLERFARPGELLVGADSHSTMAGAVGMFAVGAGGLDVAVAMAGYGFSLECPKVVG
                     VELRGELPSWVQSKDIILELLRRYGVRGGVGRVFEFTGEGGSTLSVTDRGTICNMITE
                     LGATAAVFPSDAQTSEWLAAQRREEDYVPLAADPDASYDENEVIELPQLEPLIAEPSS
                     PGNVVPVREVAGAETVQVCVGSSVNSSYEDLATAAAVLRENIVHPRIEMTVTPGSRQI
                     LDTISKSGVYQDLVAAGARMLEPVCGPCIGVGQAPSAGVPSVRTFNRNFPGRSGTSGD
                     EVYLCSPATAAATALKGEISDPRELGEPPTIAPAPSDPNMDDRQILDPPPPEEARNVE
                     IVRGPNIVPPPEGRPLPEELEGRIVIVTEDDVSTGDMAPDGALGMSLWSNIPECAKYM
                     FRRQDPEFHDRALKWGGGFIVGGHNYGQGSSREHAALAPLHLGIRAVIARSFARIHRR
                     NLISQSILPLLFREEDDYKRVSRGDTWKIEGVRELIAAGEQTLVVNTDDGTKIELEIR
                     LSPREREVLLAGGTLKFLRGA"
     gene            10947..11453
                     /locus_tag="VFQ10_05490"
     CDS             10947..11453
                     /locus_tag="VFQ10_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET9964827.1"
                     /translation="MAVKEGWRGRYFEDFEVGDVYEHPLGRTVTTTDNVWFTLLSQNT
                     APVHFDHNYAAQTEFGKPLVDSTFTLALVTGQSVTDISQNVFANLGWDEVRLPAPVFE
                     GDTIYSQSEVLEKRESRSRPNVGIVTVETTGYNQDGRTVITFKRTIMVYRRGHAPKIT
                     RITPGGDG"
     gene            11450..12823
                     /locus_tag="VFQ10_05495"
     CDS             11450..12823
                     /locus_tag="VFQ10_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET9964828.1"
                     /translation="MSVTRTATTRELASFLSGLHFEDLPDEVVERTKELFLDWAASAL
                     AGKNARPVRILERFTETMGPAEGPSEILVSRRRTSPLFAALVNGAASHVVEQDDLHNA
                     SVFHPATVVFPAALAAAQQTGASGRDLISASVAGYETGVRVGSYLGRSHYKVFHTTGT
                     AGTLAAAAAVSHLLGADEEVMLHALGSAGTQAAGLWEFLRDAADSKQLHTAKAASDGL
                     LAAYLASDGFTGATRILEGDQGLAVGMSSDADPPKLVEGLGEKWAVPETSFKFHASCR
                     HTHPAADALLEAMGEHGLGADQIAGVRARVHGAAIDVLGPVRDPRTIHQSKFSMGFVL
                     AMIATTGSAGIDDFTEEALEDPALRRFSERVEMIFDPEIDAAYPQRWIGLVEIETTGG
                     EHIISRVDVPKGDPGNTLSREELEEKARSLAAFQGGASDAEMDRIIGRVWSLDLEPDV
                     RNLLPEA"
     gene            12825..>13897
                     /locus_tag="VFQ10_05500"
     CDS             12825..>13897
                     /locus_tag="VFQ10_05500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9964829.1"
                     /translation="MGSPDQYEDLRRGVREVCADFPDGYWRELDARREYPEAFIEAMT
                     KSGYLGALIPEEYGGLGLSLTETTIILEEINRSGGNAQPAHAQVYVMGTMLRHGSEQQ
                     KREYLPKIASGQLRLQAFGVTEPEAGTDTTSLRTTAMRDGDHYVINGRKIYISRVQHS
                     DLMILLARTTPLDRVTRKSDGLSVFLIDLREAAGNGLTVKPRRVMINNETNELLFENL
                     SIPPSSLIGEEGRGFRYILDGMNAERILIAAECIGNGRWFVDKATRRATERVVFGRPI
                     GQNQGIQFPIARAHISVEAADLMRFRAAELFDGGEPCGAEANMALLLTADASWEAANA
                     AMQTFGGYSYDAEYDVERKFRETR"
BASE COUNT         2424 a         4547 c         4494 g         2432 t
ORIGIN      
        1 ccgtcgccga gtcctggagg gcgaaccctg tcgagtcgaa gacggtgatg tcgctgtcgg
       61 aggtgcgtcc ttcgagcttg ccggcgacga cctgcccgat ctcgcccgcg atgtcgtcct
      121 cagagatcac accttcccgg aagggaacgt tgatctcccc gtcggtccgg cactggcgga
      181 tgtcgtcgac gaagatgcgg gctcgttcga ggatcgtggg atccagctcc tgatcgcctt
      241 ccttgtccgc tcccacagcg gcgatgtggg tgccctcgga gatccaaccg ttctcgacga
      301 tcggtcccct ggccggagtg acggttacga cgacgtccgc gccgcgcaca atctcctcca
      361 gattggtaga cgcccagatc tcgaagtggt cgtactttgg ctgctgactc tgcacgaagt
      421 cgtcgagggt cgtttgcgag cggctccaga cgtgcacgtc ttcccaccgg aagacctcgt
      481 tgcaggttgc gagggtccct tcggccatgt tcccagcgcc gacgatggcg agaactttgc
      541 tgtcctggcg cgccaggtgc ttcgtggaga cagccgcggc ggcaccggtg cgcatcacag
      601 tgtggtagct cccgtcgcag atcgccagcg ggaacccggt ctcggggtgg gtgtagatga
      661 gaatggagaa gacggtaggg aggtcgaacc tggcgcggtt ctcctcgcgg atagagaccc
      721 acttgcaggc cgccgcctcg ggttcctcga tgtaggaggg catcacctcc cactcgcccg
      781 ggtacttgtc gagcacgatg tggcccttcg gttccatgta gtagcggccc tcgccgtgca
      841 tacggtaggc gtgctcgacg caggcattgt actcgtccgg ggtgagcagg cccatcatgt
      901 cggtgcggct caggatcagg gtcttgcggt tcctccaact ctccacaggc ttcttccttt
      961 ccatgttaga cgcagcttcc ggtgaacccg ggggcggacc gcttcagata tcgagctctc
     1021 gtagtacacg ctcggttgct tcctgaagag cggcgaggtc ttgggcgctc caggcgcggg
     1081 gacccggaac gtagtggacc gagatcacac ctatcaggcc atcgtcgcgc acgaggggcg
     1141 cgaccatctg cgccctcacg ccgtagagct ctataagctc cggtggcggg gcaggatccg
     1201 ccgtcgagca atcctcctgc acgagcggtg ttctctgttc ttccaggaac ctgatcggcg
     1261 aggagtcacg caggttgcgt atcgaggtat cgcctttgat cgagtggacg ccgggcgcca
     1321 gggcctctgc cacgacctca tcaagcccgg cgtcctgatc cagcaggtcc agacgcaggg
     1381 tcgtccggct cgcgcccgtt gatctgagaa gttcttcgag tatctcccga aacggcgtac
     1441 tttcggccat gtcactacgc cgacgcgccg gacttccggg ccacggtctc gatgtaggcc
     1501 gaggcttctt cggcggagac gacatcggcg tacttggcct ggatgtcgaa caggttggcc
     1561 tcgtgcggct cctgcgcccg gtcgccgacg cactcgcgcg ggacaagcgt cggatagccg
     1621 tattggagga ggtctatcgc cgtcgcccgg atgcagccgc tcgtcgtcgc gccgcacatg
     1681 atgaccgtgt caacgtgctg agagacgagg atcgagggta ggttcgtgcc gaagaaggcc
     1741 gaggcgccct tcttgaggat taccgtctct tcttccctgc gaccgagacg cgggtctatc
     1801 tcgacccatt tgcccccgac ctggagttcg gcaaggccgg gagccttctc gagccagagg
     1861 gagccgtctt tgaggttctc ctcgaagccg atggtggtga agatgaccgg aagttcagcc
     1921 tcgcgcgcca cgtcgaggag gcccctcgtc gcttcgacct cttttgtgag gtccgctccc
     1981 atggcgcact cgggatcggt gaagccacgg ctgaagtcga ccacgagcac agccgggcgc
     2041 gcgccgagcg tcacgctctg cccgagccgg gcgcgctggt agacctcgcg cgtctgttcg
     2101 ctgctcatgt gatcaccccg tccttccggg accgccggga agctcttcac cgaaacgggc
     2161 gcgcctcacg aacttgccgg cgccgggctt cccgactagc ctgtcgcctt ccactatgac
     2221 ctgtccccga acgagcacca cctcgggggc gccgctcacc tccgtcccct cgaagaggtt
     2281 gtagtctata ttcgagtggt gcgtctcggc ggagatcttc acccgcttct cggggtcgaa
     2341 gacgacgatg tcggcgtcgc tcccaaccgc gatggtgcct ttgcgggggt acataccgaa
     2401 gaacttcgcc gggttggtag agacgagctc gacgaagcgg ttcatcgaga ggcggccttg
     2461 ccgcacgccg aagtggtgga gcatgtgcag gcggttctcg atccccggtc cgccgttggg
     2521 gatcttggag aagtcgtccc tgccgagcgt cttctggccg tcccagttga acgggcaatg
     2581 gtcggtggag acgaccgaga ggacgtcggt ggcgagcgcg tgccagaggt gctcctggtt
     2641 ctccttgggc cgcggcggcg gcgtgtagac gtacttggct ccttcgaagt ccggctgatc
     2701 tagtgccgtc tcgtcaatga agaggtactg agtgcaagtc tcgccccagg cgtgccagtc
     2761 gttcgtgcgc gcctgcgcga tcgggtcgat cgcctccttg caggagacgt ggacgacgta
     2821 gagcggggag ccggcgatcc gcgcgagttg tatagcccgg ttcgtggctt cgccctccgt
     2881 aatgggagga cgcgtcgcgg cgtgccactt cggctcggtc ttcccctccg cgagtgcctg
     2941 cttgacgagg acgtctatgg cgtcgccgtt ctcggcgtgc accatcacga gggctcccgt
     3001 ctccgacgcg acctgcatcg tcttgaaaag cgtctcgtcg tcgaccatga tcgcaccctt
     3061 gtaggccatg aagagcttgt agctcgtcac cccctcgtcc ggcgccttcc tgaggtcttc
     3121 gagcgtcccg ccctcttcga ggtcggtgac cgcgaggtgg aagccgacgt cgatcaccgg
     3181 cttggcgcgt tcgatcttct cgtgccacgt cgcgagcgcg tccgggaggc tctgccccgg
     3241 ctcctgcaaa cagaagtcga cgtggctcgt cgtgccgccg aaggccgcag agatcgtccc
     3301 cgaggtgaag tcgtcgcagg agatcgtccc gccgaacggc atctccatgt gggtgtgcgg
     3361 gtcgatcgca cccgggatga cgtacttgcc gctcgcgtcg atcaccttgt cggcctgcat
     3421 gtcgagcgac tccccaatga gggagaccct ctcgtcctcg atgaatatgt cccccacgta
     3481 gtcgtcggcg acggtaacga tacggccgcc cttgatcagt acagacatac gctccttaca
     3541 ctcctttcta caaacaaccc cgagttctct ccctgacgaa gatatgcttc attgaccgtc
     3601 cgcggtaaac gtccttatgg cgttgagcat gatctcggcc ccgagggccg cgtccgccgg
     3661 ctccgcgtcc tcctctggcg agtggctaat gccgtccttg cacgggacga agaccatggc
     3721 gctcgggatg cggtccgcga tgctcatcgt gtcgtgagcc gcgcccgagt gcatcgccat
     3781 atacggctcg ccgctcgcct ccgccgcctc ctccagcgca gccaccacgc gcccgtccag
     3841 cggcgtcgcc gacagggtct ggcgctgcgc gtacccggcg ctcatgccgc gcttctcggc
     3901 ggcttctttc gcgaaggcgg caatgtcgcg cgcgacgccg cggaagccgt cttcgtccac
     3961 gccccggatg tcgagcgaga agcgggcccg gcccggaacg gcgtttatga ggttcgggcg
     4021 aagctcgatc tccccgaccg tcccgaccgt ccctttcccg gcctccttcg ctaggcgctc
     4081 cagctcgagc atgcactctg ccataacgat gccggcgtcc tggcggaagt ccatgggtgt
     4141 cgccccggcg tgatcgccgc gcccgtggac ggtgatgtcg gcgtgaacgt agcctgctat
     4201 ggcgttgacc acgcctatcc gattctgcgt gtcctggagc acgcgcgcct gctcgatgtg
     4261 aagctcgatc caaccgatga ggtcgtcgag catgcggatc gagtcttgcc aacgctccgg
     4321 ttcgtagccg gcctcttcgg cgtgctccca gaagctccgc ccgtcgtcta cggcgcaaaa
     4381 cttctcgcgc agctcctcct ccgtcaccct ctgcgcgatg atgcggctcc ccagcagcat
     4441 ctggccgaag cccgagcctt cttcttcgag aaaagagatg agttgaagcg gaagatcgag
     4501 cccaagctcc tcgttgaggc ggcagacctc gagcgcggtt acgacgccca ttgtgccgtc
     4561 gtacttgccg ccgtttcggt tcgagtcgca gtgcgagccg acgccgaaca ccttctcgcc
     4621 cgccgagcgg ttccgggcga cgagcgtccc tacgggatcc tcgtagtgct cgaaacccag
     4681 agtttccagc tccccaataa agtagtccaa agtcttgcgg tactcgggcg tgtaagcgta
     4741 gcgcatgatg gactcggagg agcgcgtgta atcggggccc gcgagggtct ctatgtcgcg
     4801 ctctatccgc tccgcgctct tcttgcgatc tatggtcatc ggttgggtcg ccataaactt
     4861 ctccctatct catctgccta cgatgacacc ggaacgtcga gccaggaatc gaaagaattc
     4921 cggatctcct ccctagtcgc cccggctctg aggagcaggg cgaggtagag tcgggccttc
     4981 aaaccgtcca gtggaccggc gtagatgagt ccgcgctcta gcaggtccat ctcggagccg
     5041 atgaacccgt aggtagagtg gaggacctcg ccgcccccgg ttcgcgaggc caggattacc
     5101 ggcatcttca cggcgagatc ttcgagagca tccaccgtga tggagggaac gtgcccgccg
     5161 ccaagggcct ctaccacgag cccggcgtat ccgtgccgtt ctatctcggg aagcagcctc
     5221 ccatcgtcgc ccagagtgaa ggtgtgcagc gccacgggac ggtcctgtgc gtctccgggc
     5281 agcgttatct tgtgccgtcc gaccgggcgt acaaccaccc tgggaacgtc ctcggagatc
     5341 cagcctaccg gcccgaccgg atcggagcgg aaggtcgccg ggttcgaggt gtgcgtcttg
     5401 cgcacgaacc gcgccgcgtg gatctcgtcg tttaggacga ctactgtgcc aagctcccga
     5461 gccgtctcgc tcgccgcgac ctgaattgaa gccagcaggt tggccggccc gtcggcgccg
     5521 gggagcgtgg ggttgcgcat cgcccccgtg accaccacgg gagcgtcgcg atccaccaga
     5581 aggtccaaca cgaaagctgt ctcctctatg gtgtcggtgc cctgcgtgac gaccgccccg
     5641 ctcaccccct cgtcgatgcg gtcctcgatc tccgacgcga gctcgacgag atcatccacc
     5701 gtcaactccc cggacgcagc ctgacggaac gagatcgccg agacctcggc aacctccgct
     5761 atctcgggca cgtccgagac cagcgcctcc ccggtcagcg ttggctctac gcctttcccc
     5821 ccggaatcgg ttgaagagat ggttcccccc agagacaaaa ccgccacccg cggaagcgcc
     5881 aaggctcctc ctctcgtctc cactttaccc cattagtctg acaaatatat taacggggct
     5941 ggaagcgccg atcaaacgcc gggcgggtac gcgagagacg gtatctcggg ttgagcgtga
     6001 acgtcggtct cttgtagaat gactttggtc agacaaaagg ggaagatgaa agggaaaggt
     6061 ggggtgtatg gccgagaagg aaatcttttg ctccatcggc atcgacatcg acgccgttgc
     6121 aggctggctc gggtcctacg gcggtgaaga ctcacccgac gacatctcga gaggcatgtt
     6181 ctctggcgag gtaggtacgc cgcggttgct caagctcttc gagaagtacg acctgaggac
     6241 gacctggttc atcccggggc actccatcga gacgttcccg gatcaggtaa agatggtcgt
     6301 tgacgctggg cacgaggtcg ggagccacgg ctactcccac gagaacccgg tggcgatgac
     6361 gccgcagcag gagagggacg tactggagaa gtgcctggag ctgatcgaga atgtggcagg
     6421 caggaagccg gtcggcaacg tggcgccgtg gtgggaggtc tccgccacga ccaacgagct
     6481 tcttctcgag tacgactaca agtacgacca cagtctccag caccgcgact tcaccccgta
     6541 ctacgcgcgg gtgggcgact cgtggaccaa gatcgactac tccaaggcgg ccgaggagtg
     6601 gatgaagccg ctcgtgcgcg gcgaggagat agacctcgtc aagatcgggg gcaactggta
     6661 cctcgacgac ctgccgccga tgatgttcat caagggctct ccgaacagcc acggcttcgt
     6721 caacccgcac gacatcgagg agctgtggcg cgacgagttc gactgggtct accgggagat
     6781 ggactacgct acgtactgcc tgaccatcca cccggatgtg tgcggccggc cgcagtgtct
     6841 gctgatgctc gaaaggttga tcgactacat caacggccac gacggcatcc gctggacgac
     6901 gatggaggag atcgccgacg acttcgtcga gcgctacccg aggtcccagc cgaaccacga
     6961 ccctggcatc aacatgtacg gcgtcgagac cccggccgcg gaaggggcca cctcctagga
     7021 gacttcttgt atgtgcgtgc tttgcggaca ggatttcgtc gctcgggtcc actggaccga
     7081 ccggcacgtc gaggaccggg cgcgggctgc cgtgcccggc ttcgacccgg tcgcgttcca
     7141 gggcgaccgg cgccgtgacc gcgctcaccg ggtcgtcctc accaacgagg tgctgcgcta
     7201 ctatggcctc cgcgtcgatg actggagcgg gagcaagtac gtgctccgcg accgcaaagg
     7261 gcgttcggag atcatccagg acctcggctc cctctggccc gccgcggcga agctcgcggg
     7321 ccggacccta gatcccctgg acccggtcct gcgggagatg ctgggcggag agtcggcgtt
     7381 agccgcagcg gacagcgcgt aggcccatgg agggcaagac cacgcgtccg gtgacggtcg
     7441 ttaccggttt cctcggcagc ggcaagacga cccttctcgc gagggtgctc tcggcgccgt
     7501 cgatggccaa cacggccgtc ctcgtcaacg agttcggcga ggtggggctc gaccaccacc
     7561 tcctgcgccg ggtggatgag cgcacggtct tgctcgggag cggttgtgtc tgctgcacca
     7621 cccgggagga cctcgtcgga gcgctcatgg agctcctcga catggaccag aggggcgaga
     7681 taccgcggct ggatcgcgtc gtcatcgaga cgaccggcct ggcggacccg gcccctatcc
     7741 tccacaccat ctatgccgac cccgtcctgc agcaccactt ctctgtagac ctggtactca
     7801 ccaccgtgga cgcggtcaac ggcgccctgc acctcgaccg caacccggag tcggtcaagc
     7861 aggtcgcggc ggcagataag gtcgtcgtga ccaagacgga catagcggag ccgggcatcg
     7921 taccgggcct gctgtcccag ctgggcaaga tcaacccgtc agcctggacc tgcgaggcgg
     7981 ctttcggcga ggtggacgtc cacgagctct ttcgcccgga tggcgcggga acgcacgcgg
     8041 cgccagcggc ctttactgcg gagtccggcg atcctcacga cgtgggcgac acgcactccg
     8101 tctcggtgac gttcgacggg gccatcgact ggacagcgtt cgggatctgg ttcagcatgc
     8161 tgctgaatgc ccggggtgag gacgtgttgc gtgtcaaagg acttcttgac gtcggcgagg
     8221 cagggcccgt cgtcatcaac ggcgtgcagc acataatcca tccgccggag cacctcggcg
     8281 agtggcccga cgaggaccgc cggtcccgga taatcttcat caccaggtac atccatcccg
     8341 aagagttgtt ggactccctg ctcgctttcc gcgggttgct cggagcgcga cccttacccc
     8401 tccacgccgc cgagcagaga gtaccgttcc cctagtcgct tgaaggatct cgcaaaatgg
     8461 tcgtgcgacc atcagccacg ccggtcgagt ggcgtggggt ctcagtgaaa gtcgcccggc
     8521 gaccgccaac cttcgggaat gctcgttcca gcaggcgcca ccgacacctg gggagggcgg
     8581 ccggcgatga gcgtggtaaa gaccacacca ataggagttc cgacccgatc gcggacgctg
     8641 cacgcgagca tgagcctgga ggtccccccg acgctctggg gtccggccgt gacgccgggg
     8701 tagggcggat cgaagcggag gcggtacgcg cgctcgatcg tgcccgctcc ctcgctcacc
     8761 ccgcaggcca gatccaggag ttccccgacg ctaacgttgg cagcgaggct gcttcccagg
     8821 tgccgccggg cctgcgcggt cagcgccgcg gatatgagga ggcgtctcct ctcgaggtcg
     8881 agggtaggat ccatgcgcaa gatgtgctcg ctcgtacgtg gcgggtcgcc cactctcctg
     8941 ccgatcattc taacgatacc gtctgtacct cgttgcatac ggacaggagg cgaacatgag
     9001 cgagccgcgc aaccttaccc ggaagctgct gggcgaccat ctgctagaag gcgacctcgt
     9061 ccccggggag gagatggatc ttaccgtgga ccagatcctg atcgaggacg ccactggctc
     9121 catgaccgcc ctccagttcg aggcgcttgg agcagatagc gtcgccgtcc ccctcgcggt
     9181 gatgtacgtc gatcacaatg tcttgcagat cgacgacaag aacatggacg agcatcgctt
     9241 cctgcagtct ttctcgtccc actacggcat ccaatactcc cgccccggca acgggatctc
     9301 ccactacgtc cacctcgagc gtttcgccag acccggcgag ctactggtcg gcgccgactc
     9361 gcactccacc atggccggtg cggtcgggat gttcgccgtc ggggccgggg gtctcgacgt
     9421 ggccgtcgcc atggcggggt acggcttctc tctggagtgc ccgaaggtcg tgggcgtcga
     9481 gctgcgcgga gagctgccca gctgggtgca gtcgaaggac ataatcctcg agctcttgag
     9541 acgctacggg gtacgcgggg gcgtgggacg ggtcttcgag ttcacggggg agggtggttc
     9601 tactctttcg gtgacggatc ggggcacgat ctgcaacatg atcacggagc ttggagccac
     9661 cgccgccgtc ttcccgagcg acgcgcagac gagcgagtgg ctcgccgccc agaggcgcga
     9721 ggaggactac gtcccgctcg ccgcagaccc cgatgcctct tacgacgaaa acgaagtgat
     9781 cgagctcccg cagctcgaac cgctgatcgc ggaaccgtcc tctccgggca acgtggtgcc
     9841 ggtgcgcgag gtcgccggcg cggagacggt ccaggtgtgc gtcggctcct cggtgaactc
     9901 ctcctacgaa gacctcgcca ccgccgcggc cgtcctgcgg gagaacatcg tgcacccccg
     9961 catagagatg acggtcacgc cggggtcgcg ccagatcctg gacaccattt cgaagagcgg
    10021 cgtctaccaa gacctcgtcg ccgctggcgc gcgcatgctc gagcccgtct gcggtccctg
    10081 catcggtgtc ggacaggccc cctccgccgg tgtgccctcg gtgaggacct tcaaccgcaa
    10141 cttcccgggc cgcagcggga cgagcggaga tgaagtctat ctttgctccc cggcgacggc
    10201 cgccgcgact gcgctcaagg gcgagatctc cgacccgcgc gagctggggg agccgccgac
    10261 cattgccccc gcgccgtccg acccgaacat ggacgaccgc cagatcctgg acccgccgcc
    10321 acccgaggag gcgcgaaacg tcgagatcgt ccgcggaccc aacatcgtgc ccccgcccga
    10381 gggccgaccg cttccagaag agctggaggg acgcatcgtc atagtcactg aagacgacgt
    10441 ctcgaccgga gacatggctc ccgacggagc gctcggaatg agcctctggt cgaacatacc
    10501 cgagtgcgcg aagtacatgt tccgccggca ggacccagag ttccacgacc gtgcgctcaa
    10561 gtggggggga ggtttcatcg tgggcgggca caactacggg cagggctcca gccgcgaaca
    10621 cgctgctttg gctccgctgc acctgggaat ccgcgcggtc atcgccagga gcttcgcgcg
    10681 aatccaccgc cgaaacctga tctcccagag catccttccg ctgctgtttc gtgaagagga
    10741 cgactacaaa cgggtgagcc ggggcgatac ctggaagatc gaaggcgtgc gtgaactgat
    10801 cgccgcggga gagcaaacac tcgtggtaaa cactgacgac gggacgaaga tagagctcga
    10861 gatccggctg tcgccccgag agcgcgaggt cctcctggcc ggcgggaccc tgaagttctt
    10921 gcgtggcgca taggaaaggg agtcgcatgg cggtgaaaga gggctggagg ggacggtact
    10981 tcgaggactt cgaggtaggc gacgtctacg agcacccttt aggacgtacg gtaactacca
    11041 cagacaacgt ctggttcacc ctgctctccc agaacaccgc ccccgttcac ttcgaccaca
    11101 actacgccgc acagacggag ttcggcaagc cgctcgtgga ctctaccttc accctcgcgc
    11161 tggtcacggg gcagagcgtg acggacatct cccagaacgt gttcgccaac ctgggatggg
    11221 acgaggtgag gctgccggcg cctgtcttcg agggggacac gatctactcc cagtcggaag
    11281 tcctggagaa gcgcgagtcg cgctcgcgcc cgaacgtggg gatagtgacg gtcgagacga
    11341 ccgggtacaa ccaggacggg aggacggtca tcacattcaa gcgcaccatc atggtctaca
    11401 ggcggggtca cgccccgaag atcaccagga tcactcccgg aggtgacgga tgagcgtaac
    11461 caggaccgcg acgacccgcg agctggcttc tttcctctcc ggattacact ttgaggacct
    11521 gccagatgaa gtcgtcgagc gcaccaaaga actgtttctc gactgggccg cgtcggccct
    11581 cgccggcaag aacgcgcggc cggtccgcat cctcgaacga ttcaccgaga ccatgggacc
    11641 cgccgaaggg ccgagcgaga tcctggtctc gcgccggcgc acctcgccgc tcttcgccgc
    11701 gctcgtgaac ggcgccgcgt cgcacgttgt cgagcaggac gacctccaca acgcctctgt
    11761 tttccacccg gcgaccgtgg tcttccctgc cgccctcgcc gcagcccagc agaccggggc
    11821 ctcgggacga gacctgatct ccgcctcggt ggccggctac gagaccgggg tgagggtcgg
    11881 aagctacctc ggccgctccc actacaaggt cttccacacc accgggacgg cgggcacgct
    11941 cgcagccgcc gccgccgtct cccacctttt gggcgccgac gaagaggtca tgctccatgc
    12001 gctcggttcg gccgggacgc aagccgccgg gttgtgggag ttcctgcgcg acgcggcgga
    12061 ctccaagcag ctccacaccg caaaggccgc atccgacggt cttctcgccg cctacctcgc
    12121 cagcgacggg ttcaccggtg cgacccgcat cctggaggga gatcaggggc tggccgtcgg
    12181 gatgtcctcg gacgcggacc ccccaaagct ggtcgagggg ctcggggaga agtgggcagt
    12241 gccggagacc tcgttcaagt tccacgcctc ctgccggcac acgcaccccg ccgccgacgc
    12301 actgctggag gcaatgggag aacatgggct tggcgcggat cagatagccg gcgtccgcgc
    12361 ccgcgtgcac ggcgctgcga tagatgtact cggacccgtg cgcgacccgc gcacgatcca
    12421 ccagtccaag ttctcgatgg ggttcgtact cgccatgatc gccaccacgg ggagcgccgg
    12481 gatcgacgac ttcaccgagg aggcgctgga ggacccggcg ctgcgcaggt tcagcgagcg
    12541 ggtcgagatg atcttcgacc ccgagataga cgcggcctat ccgcagcgtt ggatcgggct
    12601 cgtggagata gagacgaccg gtggcgaaca catcatctcc cgcgtagatg tcccgaaggg
    12661 ggaccccggg aacacgttga gccgcgagga gctggaggag aaagcccgga gcctcgccgc
    12721 cttccagggc ggggcctcgg acgcggagat ggaccgtatc atcggtcgcg tctggagcct
    12781 ggatctggag cccgacgtgc gaaacctgct gccggaggcg taacgtgggt agccccgacc
    12841 aatacgagga cttgcggcga ggagtccgcg aggtgtgcgc ggacttcccg gacggctact
    12901 ggcgcgagct cgacgcgaga cgggagtacc cggaagcctt tatcgaggcg atgacgaaga
    12961 gtggctacct cggcgcgctc atcccggagg aatacggggg gttgggcttg agcctcaccg
    13021 agactacgat catcctcgaa gagatcaatc gttccggggg caacgcccag cctgcccacg
    13081 cgcaggtgta cgttatgggc actatgctgc gtcacggctc ggagcagcag aagagggagt
    13141 acctgccgaa gatagccagc ggtcagttga ggctgcaggc cttcggcgtg accgagccgg
    13201 aggccgggac cgacacgacc tccttgagga cgaccgccat gcgcgacggc gatcattacg
    13261 ttatcaacgg gcgcaagatc tacatctcgc gcgtccagca ctccgacctc atgatcctgc
    13321 ttgcccggac cacgccgctg gatcgggtga cgaggaagtc tgatgggctc tccgtattcc
    13381 tcatcgacct gcgcgaagct gcgggcaacg gtctaacagt gaagcccaga cgcgtgatga
    13441 tcaacaacga gaccaacgag ctcctcttcg agaacctaag catcccgccg agcagcctga
    13501 tcggcgagga aggcagaggc ttccgttaca tcctggacgg catgaacgcg gagcggatcc
    13561 tcatagccgc cgagtgcatc ggcaacggac ggtggttcgt ggataaggct acccgccgcg
    13621 ctacagagcg cgtcgtcttc ggtcgtccga taggacagaa tcagggcatc caattcccca
    13681 tagcccgggc gcacataagc gtggaggccg cggacctcat gcggttcagg gccgcggagc
    13741 tgttcgatgg gggcgagccc tgcggagccg aggcgaacat ggccttgctg ttgaccgcag
    13801 acgcctcctg ggaggccgca aacgcggcga tgcagacctt cggcggctac agttacgacg
    13861 ccgagtacga cgtcgagcgc aagttccgcg agacccg
//
LOCUS       DASTKM010000126         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1087482, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000126 DASTKM010000000
VERSION     DASTKM010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087482"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFQ10_05505"
     CDS             <1..460
                     /locus_tag="VFQ10_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964830.1"
                     /translation="RVALAAWKERPLTGTGAGTFQYTWLEDRPGLKGVKQVHNVYLEQ
                     GTETGLFAFLALLGFVAVLVGYTGRAVWRSDPQGERRLLLAGLCSALIVYLVSSAFEW
                     HWYLPASTLFFFILAAVAVKFASREDWGAAEVTTADSGAERLRIPVEAEG"
     gene            599..1510
                     /locus_tag="VFQ10_05510"
     CDS             599..1510
                     /locus_tag="VFQ10_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9964831.1"
                     /translation="MIGAPLSRLPGGVYVDLGKGDHRSSVFLAGSGRSGTTWLSEIIN
                     HRRGYRYVFEPFNPREVGPFGHFNTKQYLRPDDQREKFLEPARLALTGGLRDSWSDRF
                     NGRIVARRRLIKDIRANLLLGWIRANFPGMPIILLLRHPCAVVASRLALGWKDNLSET
                     MEQRALVEDFLLPMEAEIRAAKADFERHLFLWCIDNYVPLKQFGPKDVHLTFYENLLA
                     RPEAELQSLFAFLGEDFDDRVYRRLRRPSSLSRKNTPCPSTEGWRSRVSATQLERTME
                     ILGLFGLDHLYGEGTMPDPSYALTDGV"
     gene            1522..2640
                     /locus_tag="VFQ10_05515"
     CDS             1522..2640
                     /locus_tag="VFQ10_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964832.1"
                     /translation="MHKPPVVILSGVRWDFLWQRHQTLATHFAGAGYPTVFVETTGLA
                     NPHLTGNTLRRVFERIRRSGSAGEKPPAERNLIVYAPLTAPPTYAAFRWMNAKLLVPR
                     VLSDLRKIAGPHPVVVAYPPTRTTLALISGLDPRLVLYDCSDDYEHFPGAPKDIGETE
                     RELLLRADLVSCTSAHLLEKARSIRPDAFLSGPAVDYERFEVLQGPGGEVSTVCFFGH
                     VSLERIDFHALRAIAEAGFEVRIVGGSVEKCLFEIPGLDYRGEVSHPGLPSALAGVDA
                     FVLPYKINALTRSISPAKTYECLATGKPVVAAPLPAMLDLAEYIYLTQRPEDYVEVLR
                     NLKRLETEEKRRTRIELARRNSWDTRFSALEEALWRHL"
     gene            2625..>2897
                     /locus_tag="VFQ10_05520"
     CDS             2625..>2897
                     /locus_tag="VFQ10_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964833.1"
                     /translation="MASSVGPRAGRIDVLGVGVDPITVDELHAEIARLVQDERLGLVF
                     NVNAHCLNLCYEDSALRDSLNGADVVFCDGAGVMLAARMLGGRIPAR"
BASE COUNT          530 a          920 c          899 g          548 t
ORIGIN      
        1 gagggtcgcc ttggctgcgt ggaaggagcg tcccctcaca ggtacggggg ctgggacttt
       61 ccagtacacg tggctggagg acagaccggg gctcaagggc gtcaaacagg tccacaacgt
      121 gtatctcgag cagggcaccg agaccggctt gttcgccttt ttggccctgc ttgggtttgt
      181 ggcggtcctc gtcggctaca cagggagagc cgtctggcgg tcggatccgc agggagaacg
      241 ccggctactt ctggccgggc tatgctccgc gctcattgtc tacctcgtct cctcggcgtt
      301 cgagtggcat tggtacctcc cggcctccac gcttttcttc tttatcctcg ccgccgtcgc
      361 agtgaagttc gcctcgaggg aggactgggg tgccgccgag gttaccaccg ccgacagtgg
      421 ggctgagcgt ctccgcatcc ccgttgaagc cgaaggatag catcggccgg atgagcgttg
      481 actatcccag ctcggtgagc gactatgatc accagctgga gcccgtggcc agggagttgg
      541 cgtgggctgg gttcctggcc tccttcgctg gcgacactct cgggcggagg ccactacgat
      601 gataggcgct cctttgtcac ggctgccagg aggtgtctat gtcgatctgg ggaaaggaga
      661 ccacagaagc tccgtctttc tggcagggag cgggcggagc gggaccacgt ggctatcaga
      721 gatcatcaac cacagaaggg gatatcgcta cgtcttcgaa cctttcaacc ccagggaggt
      781 cggcccgttc gggcacttca ataccaaaca gtacctgaga ccagacgacc agagggagaa
      841 gttcctagag ccggcccgcc tcgcgctcac gggggggctc agggactcct ggagcgaccg
      901 cttcaacggg cgcatcgtcg cgaggcggcg tttgatcaag gacataaggg ccaacttgct
      961 cctcggctgg atacgcgcga acttccctgg aatgccgatc attctcctgc tcaggcatcc
     1021 ttgcgccgtc gtggcatcca ggctcgcgct cgggtggaag gacaacctct cggagacgat
     1081 ggagcagcgg gcgttggtag aggacttcct cctgcccatg gaggcggaga tccgggccgc
     1141 caaagccgac ttcgaacgcc acctcttcct ctggtgtatt gacaactacg tccccctcaa
     1201 acagttcggg ccaaaggatg tgcacctcac cttctacgag aacctgctcg cccgtccgga
     1261 agcggaacta cagagcctgt tcgccttcct gggcgaggac ttcgacgacc gtgtctaccg
     1321 cagactgaga cgcccttctt ccctgagccg aaagaatacg ccctgtcctt ccacggaagg
     1381 gtggcgttct cgggtaagcg ccacccagct tgagagaacc atggagatcc tgggcctctt
     1441 cggcctcgac cacctctacg gcgaagggac catgccagac ccttcctacg ccctgacgga
     1501 cggagtgtag gagtctccct cgtgcacaag ccgccggtgg tgatcctatc cggtgtaagg
     1561 tgggacttcc tctggcaaag gcaccagacg ctcgccaccc acttcgccgg cgccggctat
     1621 cctaccgtct tcgtcgagac cacgggcctc gccaatcccc acctgaccgg aaacacgcta
     1681 cgcagggtct tcgagcgaat ccgccgctca ggtagcgcgg gagagaagcc accggctgag
     1741 agaaacctca tcgtgtacgc gccgctcacc gccccgccga cctacgcagc attccggtgg
     1801 atgaacgcga agctcctcgt gccacgcgtc ctcagcgacc tgaggaagat agcaggaccc
     1861 catcctgtcg tcgtggccta tccgccgacg aggacgacgc tggccttgat ttcgggcctc
     1921 gaccctagac tcgtgctcta cgactgctcg gacgactacg agcatttccc cggtgctccg
     1981 aaggacatcg gagagacgga gcgcgagctg ctcttgcgcg ccgacctcgt ctcctgcacc
     2041 tcggcacacc tgctagagaa ggctagaagc atcaggccag acgccttctt gagcggtcct
     2101 gccgtagact atgagcggtt cgaggtcctg cagggtcccg gcggagaggt cagcaccgtc
     2161 tgctttttcg gccacgtgag cctagaacgc atcgacttcc atgccttgcg ggctatcgcg
     2221 gaggcgggct tcgaagtgcg tatcgtcggc ggcagcgtag agaagtgtct cttcgaaata
     2281 cccggcctcg actacagggg agaggtgtcc catcccgggc tgccttcggc tctcgccggt
     2341 gtcgacgctt tcgtactgcc gtacaagatc aacgctctca cccgctccat ctcgccggcc
     2401 aagacctacg agtgcttggc cacgggcaag cccgtcgtgg ccgcccccct tccggcgatg
     2461 ctggatcttg ccgaatacat ctacctcacg cagaggcctg aggactacgt ggaggtcctg
     2521 cgaaacctga aacgtctcga gacagaggag aagaggcgaa cccgcatcga actcgcccgc
     2581 aggaactcct gggacacccg attcagtgcg ttggaggagg ccctgtggcg tcatctgtag
     2641 ggcctcgtgc gggcaggatc gacgttcttg gcgtgggcgt ggacccgata actgtagatg
     2701 agctgcacgc ggagatagca cgcctggtgc aggacgaaag gctcggcctc gtattcaacg
     2761 tgaacgccca ctgcctcaac ctctgctatg aagattctgc actacgggac tcgctgaacg
     2821 gtgctgacgt cgtcttctgt gacggggctg gggtcatgct ggcggcgagg atgcttggcg
     2881 ggcgcatccc ggcacgc
//
LOCUS       DASTKM010000127        11704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1088433, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000127 DASTKM010000000
VERSION     DASTKM010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11704
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088433"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFQ10_05525"
     CDS             <1..268
                     /locus_tag="VFQ10_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9964834.1"
                     /translation="SDFDDAVGLANDTRYGLTAGIATKSLRYAHEFMQRSETGLVNVN
                     LPTAGLEFQVPFGGNKESGVGGREQGPAALDFYSSWKTLSIMPL"
     gene            280..747
                     /locus_tag="VFQ10_05530"
     CDS             280..747
                     /locus_tag="VFQ10_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9964835.1"
                     /translation="MRARGFTHVSISATDLEESVRFYTDFFGMEEVPSPDFSGPVRWL
                     RIGDLQLHLFLDEDPAPERHHFALDVDDFETAYTKAEELGVRDGGRYSTVRELPDGAV
                     QMYLRDPAGNLVEVNWPDVTTLDREVIPEIQKIGGDPDAVLYFEPPGRRLAEH"
     gene            770..1255
                     /locus_tag="VFQ10_05535"
     CDS             770..1255
                     /locus_tag="VFQ10_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HET9964836.1"
                     /translation="MTQEQTEQVISDLGLPDQAIQSDELPWVPQGERVWFKPLRFDLA
                     TGRWINVLKVQGSGRVNRHRHTGGQVMGFCIKGSWHYLERDWVARPGTLVYEPPGDIH
                     TLVVDEGVEEMQTLFILEGTVQYIDDDDNLIYQDDVFSKLERYLKFCDQQGIEPQDLR
                     Y"
     gene            1255..2025
                     /gene="kduD"
                     /locus_tag="VFQ10_05540"
     CDS             1255..2025
                     /gene="kduD"
                     /locus_tag="VFQ10_05540"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HET9964837.1"
                     /translation="MGVLDDFNLDGKVALVTGAARGLGQGIARGLAEAGADVAALDIV
                     SVDETGDLVQNEGRRFHAMNVDLLESDAKLAAEVVEECVSELGRLDILVNNAGIIRRA
                     PALEFGGDDWEAVIKINLSAAFYLSQAAARHFVEEGRGGKIINTASVLSYEGGMIVPS
                     YSASKAGIANLTRALANEWAPLGINVNAIAPSYFTTELTSALREDEERNAALLSRLPA
                     GRFGEPEDLKGAAVFLASDAADYVHGTILPVDGGWLAR"
     gene            complement(2157..4958)
                     /locus_tag="VFQ10_05545"
     CDS             complement(2157..4958)
                     /locus_tag="VFQ10_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HET9964838.1"
                     /translation="MKERVWDFQEVGRLPAPGDNVAMATRRLQAGTRVSYGGSEFTLR
                     HTVLEGHRFAVEPIAEGEDLLSWGLRFGCAIRDIAPGDYACNEKILRVLRERLRVSPE
                     RGEDEDPEGTSDQGGGRVPGGQDVVGLKLPEGPNFSDAELEPYVLDEEQFRPGEQVPF
                     HDEPRTFMGYRRGAGRGVGTRNYVVVMGVTSRLTGFVRALELEMKGVADTYENVDGIV
                     CVAHTEGGEERTPNNLDLLLRTLSGFMVNPNVGAVLVLDQGGEETVTNSMLGAYLEEY
                     GYPIDDLPHEFMSLEGSFRRDLVRAKAVVRGWLEEVDAVRRTEEPASELKIGLQCGGS
                     DAFSGISANPLVAWVSKEVVRNGGIANLAETDELIGAEHFVLENVKDLDTARRFLSAV
                     ERFKERVSWHGQTAEDNPSGGNNYRGLYNISIKSLGAAMKKHHDVRIDRIIEYAERMD
                     EGGFYFMDSPGNDLESIAGQVASGANMIFFTTGNGSITNFPFVPTIKFVTTTGRYELL
                     SKDMDVNAGAYLDGTPMDELGRQTFERTLRAASGERTVGERAGHAQVSIWRDWKQTSD
                     ENLDQLRNAPEPGGEPLPVKESAPEVEFSFEAIKSGRGTVVDQVGLVMPTSLCSGQIS
                     RRIANRLNERDATLGKVTRFVALPHTEGCGVSAGSAETIYSRTVLGHLASPTVRFGLL
                     LEHGCEKTHNDYFRNRLEEAGLDPNRFGWASVQLDGGIDSVVAKVEDWFTGTLDSAED
                     LEYENAGPEALRLGLYAAGSISNEAALSLAQVTLVVANSGGTVVVPERAAVLSSPAYL
                     EAVLGDLPVENTLAYGQAVPSGKPGLHVMEAPTDHWVETATGLGATGVEVMLAHVGGH
                     PLQAHLMIPLVQASSDPETLEKHAEDLDVLLEGDPSGWNEQMLETIAAVASRAYVPRL
                     FEAGNTDFQFTRGLLGVSM"
     gene            complement(5020..5265)
                     /locus_tag="VFQ10_05550"
     CDS             complement(5020..5265)
                     /locus_tag="VFQ10_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964839.1"
                     /translation="MDEQDLQVEGRLSVREEETAFLVVREEDPNDWLARFEKDGDFPA
                     REWAENMANVYNRRLTRRPTGPPTPPGTRPASYHPEP"
     gene            complement(5397..5768)
                     /locus_tag="VFQ10_05555"
     CDS             complement(5397..5768)
                     /locus_tag="VFQ10_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9964840.1"
                     /translation="MSSERKEHVLGDLSDFPVGTHRVVKVGRREIGVFNIGGKLYGLP
                     NVCPHQTGPLCEGKPPLGTLVARGENDWKFEWVNEGEIVACPWHGLEYHVPTGRCLAY
                     PNITLRSYEVLLDGDIVKVRL"
     gene            complement(5765..6904)
                     /locus_tag="VFQ10_05560"
     CDS             complement(5765..6904)
                     /locus_tag="VFQ10_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9964841.1"
                     /translation="MAKAATAKRERKEKLEDILVVDCDVHVHESPGAIMPYCEMPWKK
                     ALETIKDVQEFYLDIPGFSPGTTVYEAKFPSGQEGTRMVHSASQMRQELDEIHVDIGV
                     LFPDHFLKIPVISQVDYASALARAYNAWLLEEWCSPENGLLGCILACPQDPVDAAREI
                     EKYSGERGMVGVFLPCAGLERLWGHRQYDPIFEAAQAADLPLLMHSVTVIHPVFPFNT
                     QGFETEFGRHICSHTFSIMANLVHMITTGVPVRYPDLRIAVTEAGISWVPFLMHKMDK
                     EYIERRREVPFLEDKPSTYLKQMYFATQPVEEPENLSDLVTMLDLYDGEDNTIFASDW
                     PHHDFDHPSKVHQIPFTNEVRRKIFGENALRLFNIDAGGNRLGVR"
     gene            complement(6925..7680)
                     /locus_tag="VFQ10_05565"
     CDS             complement(6925..7680)
                     /locus_tag="VFQ10_05565"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9964842.1"
                     /translation="MGGPLEGRVAIVTGGGGGLAEGICARLASDGAAIAAVDVSREKA
                     ERVADQVRANGASVVAVEADVSDCRSVGAMTDEVASRLGGVNILVNNAAVYPRRAWTE
                     IEEAEWDRVMAVNLKGYFLCARAVYPHMKEQGRGNIVNVASITFFIGWTLLLDYVASK
                     GGIVGFTRTLAREVGPDNINVNAIAPGAFPTDAEKIHPDQEGYNRWVLEQQSIKRRGT
                     PEDIGNLVAFLASDSSSFITGQTIGIDGGWMMR"
     gene            complement(7809..8018)
                     /locus_tag="VFQ10_05570"
     CDS             complement(7809..8018)
                     /locus_tag="VFQ10_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964843.1"
                     /translation="MNDAMQQRLATILAAAIAYLITQYITDRLVDIPDEHGIKDDAIE
                     AFLKGVTTATSTILASVLVRRLFRS"
     gene            complement(8120..9115)
                     /locus_tag="VFQ10_05575"
     CDS             complement(8120..9115)
                     /locus_tag="VFQ10_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET9964844.1"
                     /translation="MNRGLSAEDSDLEIDMQGSSLDLPTGYYLERDPDILTLRRLDGS
                     IVSAFSARGAAPEAVRQTVEETVQGEHSAVVQPTLRVRFFGHFEMLCEGETMPLGRNG
                     KALTILKYLLANHTRPVSQDHLMGWLWPESNLKKARWSLNSAIHGLRKLLSGCSSSVT
                     KNYVFLEDGYYRLSPGVRVTTDVDDFDRHHERGRKLEKDRRMREAAMEYEKAIELYRD
                     DYLIEDLYEDWTMVERERLANAYMDLLDRLAIHYMEVGQHQESIRACYKVLEKDRCHE
                     DSYRLLMQCYVRLGLRARALHQYRMCEQILGQEYGTSPSPETRSLYLKLLRDERA"
     gene            complement(9354..11393)
                     /locus_tag="VFQ10_05580"
     CDS             complement(9354..11393)
                     /locus_tag="VFQ10_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9964845.1"
                     /translation="MRQIKAASTVASPRAGLIRVALTGVVALMLALAFPGAGVPYLAA
                     GLALLFAMFLIVGRPAILLRERLVRVFLDSALVGVLVVSTGGVASPFFSLYFLAALEI
                     AWIETRPKVAVATAALVAGYPAAVAASGGIGAIGSTPVLLRTGLIALFCVAVAFLGSE
                     MQGSRKLASRLSSNLANQIDRVESDERLISRFAPVMKVLSLEGILGWTVEAAHTVAGG
                     PYAHVAALKGNHHRTILEDDFDVCPSWWHPSIQRLVLQSCREGDTVRSEEEIHGIEGF
                     MAIPLGTGEDEKWGAVIVGGGEYDAEEERVLKLLAEGISPALEDAGDAPGGLDQLTGL
                     PNRASLHRVLRRELSYSRPLTVLAMQPTIVRDHGMATGDAFLQKIGKKLADGRQRAFR
                     YGDEVFVVVLIGSDESRARQTAHALQQHVSEEAGSPDDLRLIAAVGWVFAETGDEESV
                     LDAAMRALEIAKGRPEGISGLPAGTQTPEPFVEGEHRVSGIARSLVEALEAKDPYIKE
                     HLRAVSGLALRIGSEISLPGEQMEALTSGALLHDVGKIGIPDRILQKSGRLTEEEYAE
                     IKRHPLLGVSILTPATELASALPVVRHHHERFDGKGYPDGLRGEDIPLIARIVSVADA
                     FDSMIRGRPYGYGISRKAALREIEENAGTQFDPLIVRALLEIVYAPGDRQADSAG"
     gene            complement(11423..>11704)
                     /locus_tag="VFQ10_05585"
     CDS             complement(11423..>11704)
                     /locus_tag="VFQ10_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964846.1"
                     /translation="QAYAAMVLDQPSRPGMESVEAREKLSAGIDTEFDGVVVRALLRL
                     LDTESEGYRRADDHRFVFPVPESGGGARLDVPDLRTQDGLGQILPRNSQ"
BASE COUNT         2114 a         3786 c         3551 g         2253 t
ORIGIN      
        1 cagcgacttc gacgacgccg tcgggctcgc caacgacacc cgctacgggc tgaccgcggg
       61 gatagcaacc aagtctctgc gctacgcgca cgagttcatg caacggtcgg agacggggct
      121 cgtgaacgtc aacctcccga ctgcgggcct ggagttccag gtgcctttcg gcggcaacaa
      181 ggagtcgggc gtcggcgggc gggagcaggg cccggcggcc ctggacttct actcgtcgtg
      241 gaagaccctc tccatcatgc cgctgtagag agagtcagcg tgcgggcgag agggttcacc
      301 cacgtcagta tcagcgccac cgacctggag gagtcggtcc gcttctacac ggacttcttc
      361 gggatggagg aggtcccctc gcccgacttc tccgggcctg tgcgctggct gcggataggt
      421 gatctgcaac tccacctctt cctggacgaa gatcccgctc cggagcggca tcacttcgcg
      481 ctcgacgttg acgacttcga aaccgcgtac acgaaggccg aggaactcgg cgtgagggac
      541 ggcgggcgct actccacagt cagggagctg ccagacgggg ctgtccagat gtatttgcgg
      601 gaccctgcgg gcaaccttgt cgaggtcaac tggcccgacg tgactacgct ggaccgcgag
      661 gtcataccgg agatccagaa gatcggcggc gaccctgacg ctgtcctcta cttcgagccg
      721 ccggggcgcc gactagcaga acattaagtc acagacagga ggagagttca tgacgcagga
      781 gcagacagag caggtcatct cggatctcgg tcttcctgac caggccatac agagcgacga
      841 gctgccgtgg gtgccacagg gcgagcgggt ctggttcaag cccttgaggt tcgaccttgc
      901 gacggggcgt tggatcaacg tcctcaaagt ccagggctca ggcagggtca accgccaccg
      961 ccacacgggc ggccaggtaa tgggcttctg cataaagggc tcctggcact acctggagcg
     1021 cgactgggtc gcgaggccag gcacgctcgt ctacgagccg ccgggcgaca tccacacgct
     1081 cgtcgtcgac gagggcgtcg aggagatgca gaccctcttc atccttgaag ggaccgttca
     1141 gtacatagac gatgacgaca acctgatcta ccaggacgat gtgttctcca agctggagcg
     1201 ctacctgaag ttctgcgacc agcagggcat agagccccag gacctccgct actagtgggc
     1261 gtcctcgacg acttcaacct ggacggcaag gtcgcgctgg taacgggtgc agcgcgaggt
     1321 ctcggacagg gcatcgcgcg cggcctcgcc gaagcaggcg cagacgtagc ggccctggat
     1381 atagtgtctg tagatgagac cggagacctg gtccagaacg aaggccgccg cttccacgcc
     1441 atgaacgtcg atctgctcga gtccgacgcg aagctcgccg ccgaagtcgt cgaagagtgc
     1501 gtctcggaat tgggtcgtct cgacatactc gtcaacaatg ccggcatcat ccgccgagcc
     1561 ccggcgctcg agttcggcgg ggacgattgg gaagccgtca taaagatcaa cctgagcgcc
     1621 gccttctacc tgtcccaggc cgccgccagg cacttcgttg aggaaggccg gggaggtaag
     1681 atcatcaata cagcctcggt cctctcctac gaaggcggca tgatcgtgcc ctcctactct
     1741 gcgtccaaag cgggcatcgc caaccttacc cgcgccctcg ccaacgagtg ggcgcctctg
     1801 gggatcaatg tcaacgccat cgccccgagc tacttcacca cagagctgac gagcgcgctg
     1861 cgcgaggacg aggagcgcaa cgcagcactc ctgtcccgtc tccccgccgg acgcttcggc
     1921 gagcccgagg acctcaaggg cgccgcggtc ttcctagctt cggacgccgc tgattacgtg
     1981 cacggcacga tcctgcccgt agacggcggc tggctggcac gctaacggca ctcgggaacc
     2041 agccatcgcg cacggatctg ccactactcg taattcccgc tttcgcggga attacgtatg
     2101 ggaggaaccg catgcgtgcc cgtggcacga acgcaccccg ttcaaacgga ctagtatcac
     2161 atagagacgc cgagcaggcc gcgggtgaac tggaagtcgg tgttaccagc ctcgaacagc
     2221 ctggggacat acgcccgcga ggccaccgcc gcgatcgtct ccagcatctg ttcgttccag
     2281 ccggatgggt ctccctcgag aagcacgtcg aggtcctcgg cgtgcttctc caacgtctca
     2341 gggtcggagg aggcctgtac gagcgggatc ataaggtgtg cctgcagagg atgcccaccc
     2401 acatgcgcga gcatgacctc gactcccgtc gccccgagcc cggtcgctgt ctccacccag
     2461 tggtccgtcg gcgcctccat gacgtgcaac cccggcttgc cggaagggac ggcctgcccg
     2521 taagcgaggg tgttctccac gggaaggtcg ccgagcacgg cttccaggta ggccggcgaa
     2581 gagagtactg cagccctctc ggggacgacc accgtcccac ctgagttcgc gacgacgagc
     2641 gttacctggg cgaggctcag ggcggcctcg ttcgagatgg agccggcagc gtaaagcccg
     2701 agccgcaagg cttccgggcc tgcattctcg tactcgagat cttcggcact atccaaggta
     2761 ccggtaaacc agtcctctac cttggcgacc acgctgtcta tgccaccgtc gagctggacg
     2821 ctcgcccacc caaagcgatt cgggtcgagg cctgcctctt cgaggcggtt cctgaagtag
     2881 tcgttgtggg tcttctcgca gccgtgctcc aggagaaggc cgaacctcac ggtggggctg
     2941 gcgaggtggc cgagcacggt gcgggagtag atggtctccg ccgagccggc ggagacgccg
     3001 cagccctccg tgtggggcag ggcgacgaag cgggtcactt tgcccagggt ggcatcgcgt
     3061 tcgttcagcc ggttggctat gcggcgcgag atctgacccg agcaaaggct cgtaggcatg
     3121 acgagcccga cctgatccac tacagtgccg cgcccgctct ttatggcctc gaaggagaat
     3181 tcgacctcag gagcactctc ctttacgggc aacggctctc cgccgggctc cggcgcgttc
     3241 cgaagctggt ccaggttctc gtcgctcgtc tgcttccagt cgcgccagat cgagacttgg
     3301 gcgtggcccg cacgctctcc aacggttctc tcgcccgagg cggccctcag cgttcgctcg
     3361 aatgtctggc ggccaagttc gtccatcggg gtaccgtcca ggtaggcgcc ggcgttcacg
     3421 tccatgtctt tggagaggag ctcgtagcgg cctgtggtgg tgacgaactt tatggtcggg
     3481 acgaagggga agttggtaat ggagccgtta cccgtggtga agaagatcat gttcgcccca
     3541 gaggcgacct gcccggcgat gctctccagg tcgttgcctg ggctgtccat aaagtagaag
     3601 ccgccctcat ccatcctctc tgcatattcg ataatgcggt cgatgcgcac gtcatgatgc
     3661 ttcttcatcg cagcccccag agacttgatg gagatgttgt agagtccgcg gtagttgttg
     3721 cccccagatg ggttgtcttc ggctgtttgc ccgtgccagg agacgcgctc cttgaagcgc
     3781 tcgactgcag agaggaaacg ccgcgcggta tcgaggtcct tgacgttctc caagacgaag
     3841 tgctcggccc cgatcaactc gtccgtctcc gccagattgg cgatgccgcc gttccttact
     3901 acctccttgc tcacccacgc caccagcggg ttcgcggaga tcccggagaa ggcgtcggat
     3961 cctccgcact ggagtccaat cttgagctcg gacgccggct cctcggtgcg ccgcaccgcg
     4021 tccacctcct ccagccatcc ccgcacgacg gctttggccc ttacgagatc ccgcctgaag
     4081 ctcccctcca ggctcatgaa ctcgtgcggc aagtcgtcta tcgggtatcc gtactcttcc
     4141 aggtacgccc cgagcatact gttcgtcaca gtctcctctc cgccttggtc gagcacgagc
     4201 acggccccca cgttcgggtt gaccatgaaa ccgctcaggg tccgaaggag caaatccagg
     4261 ttgttcggtg tgcgctcctc cccgccctcc gtgtgggcaa cgcagactat gccgtctacg
     4321 ttctcgtacg tgtcggcgac gcctttcatc tcgagttcca gcgccctgac gaacccggtg
     4381 agcctcgaag tgacgcccat cactactaca tagttacgcg tcccaacgcc cctgcccgca
     4441 ccgcgcctgt agcccatgaa ggtgcgtggc tcgtcgtgga aaggaacctg ctctccgggg
     4501 cggaattgct cctcgtcgag gacgtagggc tcaagttcgg cgtcgctgaa gttcggccct
     4561 tcgggcagct tcaggccgac cacgtcctgc cctcccggaa cacgcccccc gccctgatcg
     4621 gaggttcctt caggatcctc gtcttcgccg cgttcaggcg agacccgcaa acgctcgcgc
     4681 aggacgcgca ggatcttctc gttgcatgcg taatcgccgg gcgcgatgtc ccttattgca
     4741 caaccgaaac ggagacccca ggagagcaga tcctcccctt ctgctatggg ctcgacggcg
     4801 aagcggtggc cttctaggac cgtgtgtctt aaggtaaact ccgagccgcc gtaggacacg
     4861 cgcgtccccg cctgcagcct gcgggtcgcc atagccacgt tgtcgcccgg cgcggggagg
     4921 cgtccgactt cctggaaatc ccatactcgt tccttcacag cagtccctct cttgtccttt
     4981 cagacatacg ttctcgcgac gagtgtatcg cagcaggccc tacggctcgg gatggtaact
     5041 ggcaggacgc gtgccaggag gtgtgggagg tccggtaggg cggcgagtga ggcgtctgtt
     5101 gtagacgttg gccatgttct cggcccactc acgggctggg aagtctccgt ctttctcgaa
     5161 gcgggccagc cagtcgttcg ggtcctcctc cctgactacc aggaacgccg tctcttcctc
     5221 gcgtacggag agtcggcctt ctacctggag gtcttgctcg tccattccgc ctctacctcg
     5281 cctagctcgg ccagccgggg cacgaggaag gtgcccacgt gcctgctacc cccgatcacc
     5341 acgacgcgca tcagctgtct cctgataatc tccgagatcc gacccaacta cgccagctag
     5401 agcctgacct tcactatatc tccgtccagg agcacctcgt aggagcggag ggtgatgttc
     5461 gggtaggcga ggcatcttcc ggtcgggacg tggtattcga gcccgtgcca ggggcaggcc
     5521 acgatctcac cctcgttcac ccactcgaac ttccagtcgt tctcgcccct ggcgaccagg
     5581 gtgcccaggg gcggcttgcc ctcgcacagc ggacccgtct gatgtggaca gacgttgggg
     5641 aggccgtaga gcttgccgcc gatgttgaag acaccgatct cacgcctccc taccttcacg
     5701 accctgtgag taccgaccgg gaaatccgag aggtcgccca agacgtgctc cttgcgctcg
     5761 ctactcatcg cacccctagg cggttgccgc cagcgtcgat gttgaagagt cgcagggcgt
     5821 tctcgccgaa gatcttgcgc ctgacctcgt tcgtgaaggg gatctggtgt accttcgagg
     5881 ggtggtcgaa gtcgtggtgc ggccagtcgg aggcgaagat ggtattgtcc tccccatcgt
     5941 agaggtccag catcgtcacc aggtcggaga ggttctcggg ctcctcgacg ggctgggtgg
     6001 cgaagtacat ctgcttcagg taggtgctcg gcttgtcttc gaggaaagga acctcacggc
     6061 ggcgctcgat gtattccttg tccatcttgt gcatcaggaa aggcacccag gagatgccag
     6121 cctcggtgac ggcgatgcgg aggtcgggat accttaccgg tacgccggtg gtgatcatgt
     6181 gcactaggtt cgccattatg gagaaggtgt ggctacagat gtgccgcccg aactccgtct
     6241 cgaacccctg ggtgttgaaa gggaagacgg gatggatgac ggtcacgctg tgcatgagca
     6301 ggggcaggtc cgccgcctgg gcggcctcga agatcgggtc gtactgcctg tgcccccata
     6361 gccgctccaa cccggcacag ggcaggaaca caccaaccat gcccctttct cctgagtact
     6421 tctctatctc tctggctgcg tccacggggt cctgcgggca ggcgaggatg cagccgagca
     6481 ggccgttctc gggcgagcac cactcctcga ggagccaggc gttgtaggcg cgggcaaggg
     6541 ccgaggcgta atccacctgg gagatcaccg gtatcttcag gaagtggtcg ggaaacagga
     6601 caccgatgtc cacgtggatc tcgtcgagct cctggcgcat ctgggaggcg gagtggacca
     6661 tgcgcgtgcc ctcctgcccc gatgggaact tcgcctcata gacggtcgtc cccggcgaga
     6721 agccagggat gtccaggtag aactcctgca cgtcctttat ggtctccagc gccttcttcc
     6781 agggcatctc gcagtagggc atgatcgcac ctggcgactc gtggacgtgg acgtcgcagt
     6841 ccacgacgag tatgtcctcg agcttctctt tacgttccct ctttgccgtt gcggctttgg
     6901 ccacggtccc tccctgatcc cgtcttagcg catcatccaa ccaccgtcga tgccgatggt
     6961 ctggcctgtt atgaaggagg atgagtcgga ggctaggaac gcgacgaggt tgcctatatc
     7021 ttccggtgtg ccgcggcgct tgatgctctg ttgctcgagc acccagcggt tgtagccttc
     7081 ctggtcgggg tggatcttct cggcgtccgt ggggaaggcg cccggcgcta tggcgttgac
     7141 gttgatgttg tccgggccga cctcgcgggc gagcgtgcgc gtaaagccga ctatacctcc
     7201 tttcgaggca acgtagtcga gcaggagcgt ccacccgatg aagaaggtga tcgaggccac
     7261 gttcacgatg ttgccccgtc cctgctcctt catatgagga tagacggccc gcgcgcacag
     7321 gaagtagcct ttgaggttaa cggccatcac cctgtcccat tctgcttctt cgatctccgt
     7381 ccacgcccgc cgtgggtaga cagcggcgtt attgacgagg atgttcacac cgccgagcct
     7441 gctcgcaact tcatcggtca tggccccgac cgaacggcag tcagagacgt cagcctccac
     7501 ggccacgacg ctggcgccat tcgctctgac ctggtctgcg accctctccg ccttctcgcg
     7561 ggagacgtcg accgcggcga tggccgcgcc gtccgaggcg agtctcgcgc agatgccctc
     7621 ggcgaggcct ccgcccccac ccgttacgat agcgacccga ccctccagag gacctcccac
     7681 gaaaaccaac ctcctagacc gaccacgcgc cctctacccg ccgacgatga tatacacctt
     7741 ataggaccgc aacaagaaag ccggaacccc gccccgggct ccggcgattc tagagcttcg
     7801 cgtaagcttc aggatctgaa cagccgccgc acgaggaccg aggccaggat ggtcgaggtc
     7861 gcggtcgtca cccccttgag aaacgcctct atggcgtcgt ctttgatccc gtgctcatcg
     7921 ggtatatcta cgagtctgtc ggtgatgtac tgggtgatga ggtaggctat cgcggctgcc
     7981 agtatggttg cgagtcgctg ctgcatcgcg tcgttcatac gctcccttcc gcctttttag
     8041 gtccgctctg gtcggtaccc acgataggct ccggggaaac ccccgcgggt tctggtcttc
     8101 gaatccggtc aggcagctct cacgcgcgct cgtctctcag gagtttcagg tagagggatc
     8161 tggtctccgg cgagggtgag gtaccgtatt cttgtccgag gatctgctcg cacatcctgt
     8221 attggtgcag ggctctggcc cgcaggccga ggcggacgta gcactgcatc aggaggcggt
     8281 agctgtcttc gtggcaacga tccttctcaa gtaccttgta acaggcgcgg atgctctcct
     8341 gatgttgccc gacctccatg tagtggatgg ccagccggtc cagaagatcc atgtaggcgt
     8401 tggcaagtcg ctcacgctcg accatggtcc aatcctcgta caggtcttct atcaggtagt
     8461 cgtcccgata cagctcgatg gccttttcgt actccatggc cgcctcccgc atccgacggt
     8521 ccttctccag tttacgccct cgctcgtggt gtctatcgaa gtcgtccacg tccgttgtca
     8581 ccctcacgcc cggagacaga cgatagtatc cgtcctcgag gaagacgtag ttcttggtta
     8641 cagaagacga acatccgctc aaaagcttgc gcaagccgtg gatagcggag ttgagggacc
     8701 accgggcttt cttgaggttg gactcaggcc acagccagcc catgaggtga tcctgcgaga
     8761 caggacgggt atggttggcc agcaggtatt tgagtatggt cagcgctttg ccgttacggc
     8821 cgagtggcat agtttcaccc tcgcagagca tctcgaagtg cccgaagaaa cgcacccgca
     8881 aagtaggctg cacaacggcg gaatgttctc cctggacggt ctcctcgacc gtctgccgca
     8941 cggcctccgg cgcagcacca cgggccgaaa acgcgctgac tatggacccg tcgagtcggc
     9001 gtagggtcaa tatgtctgga tctctttcca gatagtaccc ggtcggcaga tctaacgatg
     9061 atccctgcat gtctatctcc agatcgctgt cctcggccga aaggcctcgg ttcacaactc
     9121 cctaacccgt attgcagtgg ttacgcgcag acgttctgtg gaccaaccgc tgaggctcct
     9181 ttcccacggg acgaagcctc agagtcttcg gctcgcccgg aaccacattg cgagttcagc
     9241 atataacaga gatggggttg cccgtatcac tcaaatgagt gatttttcat ccgtttcgaa
     9301 aattatatgg tagagacgcc ggtcagagca aatcgcgcgt ccggtacgcg tcgctaacca
     9361 gcggaatcgg cctgccggtc gccgggagcg tacacgattt ccagaagcgc ccttacgata
     9421 agaggatcga actgggtacc cgcgttctcc tcgatctccc tcagagcggc tttgcgggag
     9481 atgccgtaac cgtacggcct gccacggatc atggaatcga acgcgtcggc caccgagact
     9541 atcctagcta tcaagggaat atcctcaccg cgcagcccgt ccgggtatcc tttgccgtcg
     9601 aagcgctcgt gatggtgtct gacgaccggc aacgcggatg ccagctcagt tgcaggggtg
     9661 aggatggaga caccgagtag cggatgccgc tttatctccg cgtactcctc ctctgtcagg
     9721 cgacctgact tttgcaagat gcgatccggt atgcctatct tacctacgtc gtgcagcaga
     9781 gccccgctgg tcagggcttc catctgttcg cctggcaagg agatctcgct gcctatgcgc
     9841 agggcgagcc ccgacacagc cctcagatgc tccttgatgt aagggtcttt cgcctccagg
     9901 gcctcgacga gcgatctggc gatccccgag actcgatgct cgccttcgac gaacggttcg
     9961 ggggtctgtg tccccgcagg taacccggag atcccttcgg gccggccttt ggctatttcc
    10021 agggcgcgca tggccgcatc gagaaccgat tcctcgtctc ctgtctcggc gaacacccag
    10081 cccacggcgg cgatcaatcg cagatcatca ggactaccgg cttcctccga gacatgttgc
    10141 tggagcgcgt gcgctgtctg gcgggccctg ctctcgtccg aaccgatcag gacgacgacg
    10201 aacacctcgt ccccgtagcg aaacgcgcgc tgcctgccgt ccgcgagctt tttaccgatc
    10261 ttctggagga aggcatcacc cgtggccata ccgtggtcac gaacaatggt cggttgcatc
    10321 gccaagacag taagcggcct gctataggag agttcccggc gcagaacgcg gtgcagcgac
    10381 gcacggttag gcaaccctgt gagctggtcc agcccgccag gggcgtcccc ggcgtcttcc
    10441 agggcagggc ttattccttc ggcgaggagc ttcaggacac gctcctcctc ggcgtcgtac
    10501 tcaccgccac cgacgatcac cgccccccac ttctcgtctt cgcctgtacc caggggtatg
    10561 gccatgaaac cctctatccc gtggatctcc tcctcgctgc gcacggtgtc gccctcgcgg
    10621 caactttgca gcacgagacg ctggatcgag ggatgccacc aactggggca gacgtcgaag
    10681 tcgtcctcca gtatggtcct gtgatggttg cctttcagtg cggcaacgtg cgcgtacggc
    10741 ccgccagcta ccgtgtgggc cgcctcgacg gtccatccga gtataccttc caggctcaag
    10801 accttcatta cgggtgcgaa cctcgagatc agacgctcgt cgctctccac tcggtctatc
    10861 tggtttgcca ggttggagga gagtctgcta gcgagcttcc tggaaccctg catctcggag
    10921 cccaggaacg cgacggcaac acagaagaga gcgatgaggc cggtcctcaa gagcacaggg
    10981 gtagaaccga tcgcaccgat gcctcccgag gcggcgaccg cggccgggta cccggccacc
    11041 aacgcggccg tcgccaccgc gaccttcggc cgcgtttcga tccaggctat ctccagcgcc
    11101 gcgaggaagt agagagagaa gaagggtgag gccactcccc cagtggacac aaccagcacc
    11161 ccgacgaggg cggagtccag aaagacgcgt acgagcctct cccgcagaag gattgcgggt
    11221 cgcccaacga tcaagaacat cgcgaagagt agcgcgagtc cggcagcaag atagggaact
    11281 ccagccccgg gaaaagccag agccaacatg agggctacga cccccgtcag cgccacacgt
    11341 attagtcctg ctctgggcga ggccaccgtg gatgccgcct tgatctgacg cattctcctg
    11401 cctcaccccc ctctatctga gactactgac tgttacgggg gaggatctgg ccgaggccat
    11461 cctgggtgcg caggtcgggt acgtcgagcc tggcgcctcc cccgctctcc gggacaggga
    11521 agacgaaccg gtggtcgtcg gctctgcggt acccttccga ctcggtatcg aggagcctca
    11581 acagcgcccg cacaaccacc ccgtcgaact cagtgtctat gcccgcgctc aacttctccc
    11641 gcgcctccac ggactccata ccgggacgac tgggctggtc gagcaccatg gcagcgtagg
    11701 cctg
//
LOCUS       DASTKM010000128         5437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_207380, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000128 DASTKM010000000
VERSION     DASTKM010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207380"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VFQ10_05590"
     CDS             <1..1008
                     /locus_tag="VFQ10_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET9964847.1"
                     /translation="VHGLTGVCPSVAIHIPWDRVDDYGELKGHAESLGLRIGAVNPNL
                     FQEPEYKLGSVSNPDPGVRRRATDHLLECVEIADATASDVLSLWFADGTNYPGQDSFT
                     ERRHRMIEALGEVYAAMPERMRMLLEYKPYEPAFYHTDLADWGAALTVGQRLGERAQV
                     LVDLGHHAKEVNIEYVVALLLDEGRLGGFHFNNRKYGDDDLIVGSVNPFELFLIYVEL
                     AEAEEHSDTRIDYMIDQTHNIEPKIEAMLLSVMNLQEAYAKALLVDRAALKEARSAGD
                     VLGAHRTLLDAYATDVRPLCARVRETLGGASDPVAAFEESQYAGKVAEERGEGAGAGW
                     S"
     gene            1175..3259
                     /locus_tag="VFQ10_05595"
     CDS             1175..3259
                     /locus_tag="VFQ10_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HET9964848.1"
                     /translation="METRIPGATENLWDQQNAEGLSELEALAYRSNLLGRDRAVANYG
                     GGNTSTKARERDHTGREVDVLWVKGSGSDLATIEAGQFTGLKLDEVLALEEREETSDE
                     EMVAYLASCQLRPDMPRGSIETLLHAFVPYAQVDHTHPDAINMICCAEGGEELARECF
                     GDEAVWIPYIRPGFTLSKQVGEAVRNNPNARFVLLAKHGLVTWGETHEESYGRTIESI
                     NRAAEFVAGRAGEPFGGRKVQPPAPDRREALLTKVLPALRGALSSGLQEQAHKILRTD
                     CTSEDALAFVCGRDSEQLSQVGAACPDHLVRTKFRPLWVDFDPENEDADELSARLREG
                     VARYREEYEAYFSRYEEADEEMFDPNPRVILIAGLGLVAAAKNAKEADLSRDFVYRAI
                     NVIRGAHALGGYVSLTEEESYAVEYWPLELYKLTLAPPPDELAGRIAFVTGGAGGIGS
                     AVARSLASRGACVVVCDLDEEGAGEVADSLAEPGIPAKADVTDEEEVAHAYQRAILEY
                     GGVDVVVSNAGLASSAPIEETSVEMWDKNYGVLTKGYFLVAREAFRIMKEQGMGGSLV
                     FVASKNSMAAGKNASAYSSAKAAELHLARCLAEEGGPERIRVNTVNPDAVLQGSRIWD
                     SSWREERAAAYGIEPDELEDYYRWRNVLKVNVLPENVAEAVLHFASEARSSRSTGNVL
                     NVDGGVKDAYPR"
     gene            complement(3314..3994)
                     /locus_tag="VFQ10_05600"
     CDS             complement(3314..3994)
                     /locus_tag="VFQ10_05600"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET9964849.1"
                     /translation="MSNRRSVVGVGSAQRFERRSALSALALAGIAGPIIFTVVALMHS
                     LLREDHSLTEHPVSALAAGPSGWIQNVNFLLFGSLMIAYAIGLHLGVYPSRWGFVGFA
                     LLVLSGIGLMWGGLFPATDATGSFDEDRLLHIPGFIMTFLGGEIGLIVMSRRMARDPR
                     WKSLATYALLSGIAILVLIVVGGGLVRPPGSPLHAWFGLFQWVLLAVWLPCTVVLAIR
                     LLRLARVA"
     gene            4324..4713
                     /locus_tag="VFQ10_05605"
     CDS             4324..4713
                     /locus_tag="VFQ10_05605"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HET9964850.1"
                     /translation="MLADARPIPEQYHEARWWYKDKEGWMTQYAWVLEVRPGYEEEYV
                     RRHQEIWPEMVGALREAGIRNYNIFRYGLTLFGYFETDDIEKTQEYLANSETNQRWSE
                     WMDPIMKVDLDPTTQFPYLLPLQWHMD"
BASE COUNT         1186 a         1626 c         1727 g          898 t
ORIGIN      
        1 gtgcacggtc tcacaggcgt atgtccctcg gtggcgatac acatcccgtg ggacagggtg
       61 gacgactacg gcgaattgaa aggtcacgcc gagtctctgg gtctgcgcat aggggccgtc
      121 aaccccaacc tcttccagga acccgagtac aagctcggca gcgtcagcaa cccggatccg
      181 ggggtgcgca ggagggccac ggatcacctc ctcgaatgcg tcgagatcgc ggatgcgaca
      241 gcctcagacg ttctctctct atggttcgcc gacggcacga actaccctgg ccaggactcc
      301 tttacggagc gccgccacag gatgatcgag gctttaggtg aggtctacgc cgccatgccc
      361 gagcgcatga ggatgctcct cgagtacaag ccctacgagc cggccttcta tcacaccgac
      421 ctcgccgact ggggcgcagc ccttaccgtg ggtcaaagac tgggcgagag ggcacaggtt
      481 ctcgtggacc tgggccacca cgccaaagag gtcaacatcg agtacgtcgt ggcgctcctg
      541 ctggacgagg gacgtctcgg gggtttccat ttcaacaaca ggaagtacgg cgacgacgac
      601 ctgatcgttg gcagcgtcaa cccgttcgag ctgttcttga tctacgtcga gctcgccgag
      661 gccgaggagc actccgatac gcggatagac tatatgatcg accagacaca caatatcgag
      721 cccaagatag aagctatgct cctctctgtg atgaacctgc aagaagccta cgcaaaggcc
      781 ctcctcgtag accgcgcagc cctcaaagaa gcgcgaagcg ccggcgacgt gctcggcgcc
      841 caccgcaccc tgctcgacgc ctacgctaca gacgtgcgcc ctctgtgcgc cagagtccgc
      901 gagaccctgg gcggtgcctc agaccccgta gccgccttcg aagagagcca gtacgccggg
      961 aaagtagcgg aagaacgtgg cgaaggcgca ggagcaggat ggagttagag cggtctagct
     1021 atcggcggtc agccctcggc cacgctatgg acggcgaacg cactctctgt tccagaagcc
     1081 gcggaagtac acatgcgcca caagacgctt gcgaaccacg atcggatcac aaaggagctg
     1141 aaagctgaca gcgccgacca gagggagggc acacatggag acgcgaatac caggagcgac
     1201 agagaacctg tgggaccagc aaaacgctga ggggctctcc gagctcgaag ccctagccta
     1261 ccgctccaac cttttaggga gggacagggc tgtcgccaac tacgggggcg gcaacacctc
     1321 taccaaggcc agagagaggg accataccgg gcgtgaggtt gatgttctgt gggtgaaagg
     1381 ttcgggatcg gacctggcga ccatagaggc tgggcaattt acgggtctca agctcgacga
     1441 agtactcgcg ctcgaggagc gggaggagac gagcgacgag gagatggtcg cctacctcgc
     1501 cagttgccag ctccgacccg acatgccgag gggctccatt gagacgctcc tgcacgcttt
     1561 tgtcccttac gcgcaggtcg accacacgca ccccgatgcg atcaacatga tctgctgcgc
     1621 ggaaggcggc gaggagctcg cgcgcgaatg cttcggcgat gaggcggtgt ggatcccata
     1681 cattagacca ggctttaccc tctcgaagca ggtgggcgag gccgtaagga acaaccccaa
     1741 tgcgaggttc gtcctgctcg ccaagcacgg gctcgtaacc tggggggaga cccatgagga
     1801 gagctacggc cgcaccatcg agtccataaa ccgggccgct gagttcgtcg ccggccgggc
     1861 tggcgagccc tttgggggca ggaaggtcca accgccagcg cctgaccgca gagaagccct
     1921 tctcaccaaa gtcctgcctg cgttgcgcgg ggcgctctcg tcggggctcc aggagcaggc
     1981 acacaagata ctgcgtaccg actgcacctc agaagatgca cttgccttcg tctgtggccg
     2041 cgactcggag caactctccc aggtcggcgc agcctgccct gaccatctgg taaggacgaa
     2101 gttcaggcca ctgtgggtgg acttcgaccc agagaacgag gatgcggacg agctgagtgc
     2161 gagactccgc gagggcgtcg cccgttacag agaggaatac gaggcgtact tctcgcgcta
     2221 tgaggaggct gacgaggaga tgttcgaccc taatcccagg gtaatcctca tagcgggctt
     2281 aggactcgtc gccgccgcga agaatgccaa agaagcagat ctctccagag acttcgtcta
     2341 cagggcaatc aacgttatac gcggcgccca cgccttgggc ggctacgtct ccctcacgga
     2401 agaagaatct tacgcggtcg aatactggcc gctcgagctc tacaagctca ccctcgcccc
     2461 gccgcctgac gagctcgcgg gcaggatcgc cttcgttacg ggtggggcgg gaggcatagg
     2521 cagcgccgta gccaggtcgc tggcctcacg cggggcctgc gtcgtggtct gcgatctcga
     2581 cgaagagggg gcgggtgaag tcgcggatag cctcgccgag cctggcatcc cggcgaaggc
     2641 cgacgtgacc gacgaggaag aggtggccca cgcctaccag cgagctatcc tggagtacgg
     2701 cggggtcgac gtcgtcgtat cgaacgccgg cctcgcctcc agcgccccca tcgaggagac
     2761 gagcgtcgag atgtgggaca agaactacgg cgtactgacg aagggatact tcctcgtcgc
     2821 gcgcgaggcc ttccgcatca tgaaggagca gggcatgggt gggagcctcg tcttcgtcgc
     2881 ctcgaagaac tctatggcgg cagggaagaa cgcatcggcg tactcctcgg cgaaggcggc
     2941 cgagttgcac ctggcgcggt gcctggccga ggagggagga cccgagcgca taagggtcaa
     3001 caccgtcaac cctgacgcgg tgttgcaggg gtccaggatc tgggactcaa gctggcgcga
     3061 ggagcgcgcc gccgcgtacg gtatcgagcc ggacgagctc gaagattact accgctggcg
     3121 caacgtcctg aaggtaaacg ttctgcccga gaacgttgcc gaggccgtgc ttcacttcgc
     3181 ctccgaggcc cgttcgtcga ggagcaccgg caacgtcctc aacgtcgacg gcggcgtgaa
     3241 agacgcatac cccagataga ggtgggtgag gacatacaac agctgccttc ctcctgctcg
     3301 gtcagcgtgg cctctaggcc actctcgcca gacgcagcag tctaatcgcg aggactaccg
     3361 tgcaaggcaa ccacaccgcc agcagcaccc actgaaacag gccaaaccac gcgtgtagcg
     3421 gtgaccctgg cggtcgcacc aggccacctc ccaccacgat caggaccagt atggctatgc
     3481 cgctcaggag tgcgtaggtg gccagactct tccatctcgg gtcgcgcgcc atgcgccgcg
     3541 acattacgat taggccgatc tcaccaccga ggaaggtcat aatgaagccg gggatgtgca
     3601 acaagcgatc ctcatcgaaa gagccggtgg catccgttgc aggaaacaac cctccccaca
     3661 taagtcctat gccgcttagc accaacaacg cgaagcccac aaagccccat cggctcggat
     3721 acaccccgag gtggagccct attgcgtatg cgatcatgag cgacccgaaa aggaggaaat
     3781 tcacattctg gatccagccg ctgggccccg ctgccagcgc gctgaccgga tgctccgtaa
     3841 ggctgtgatc ttctcggagc aagctgtgca tcagggcaac tacagtaaag atgatggggc
     3901 cagcgatgcc agcgagggcc agtgctgaga gtgccgagcg gcgttcgaag cgttgggcag
     3961 aacccactcc gaccacactg cgccggttgc tcatttgttc ccccttggct ctgggataga
     4021 gtggaggggg cagagtctat gcgccccgtc ctaacagcgt tacgcaagca gtttacgcct
     4081 cttgtatcag gacgcgcatc ccccatttgg agtaaatctg ctcttgcttg ggagttacat
     4141 gcgggtaata gccgctagag tacattgcgg accgcaacgc gcccgagagg gtagtcctca
     4201 ccctcaggta gtaggcgggg ggacctgccg atcggccctg acggaaggta gcgcaatacg
     4261 aatgtagaaa tgaggctggt acacctcggg ctcgtaccgc ttgtccccta aatgggggga
     4321 tgcatgctcg ccgacgcgag gccgattccg gagcagtatc atgaggcgcg gtggtggtac
     4381 aaagataagg agggctggat gacccagtat gcgtgggttc tggaggtcag accggggtac
     4441 gaagaagagt acgtgcgccg ccaccaggag atctggccgg agatggttgg ggccctcagg
     4501 gaggccggta tccgcaacta caacatcttc cgctacggcc tcaccctctt cggctacttc
     4561 gagaccgacg acatcgagaa gacccaggag tacctggcga acagcgagac caaccagcgg
     4621 tggagcgagt ggatggaccc catcatgaaa gtagacctcg accccaccac ccaattcccc
     4681 tatctcctcc ccctccaatg gcacatggat tgatctaact tccggctgac gagcaaccct
     4741 tcctatcgtc ggggtttgct ccgcgccctg catggcgata tggagtgcgg accccccttg
     4801 aagatcggta gccatcgatt cctgatagtc gcgcaaagcc aagtagattt tgtcgacaag
     4861 caggaaagga agtgtagatc gagggagcgc ccggcgcgct ttcgatcgga ttcactgccg
     4921 tcaaacctgt acgatggttc aggccaaaaa ccgtcccccc tcccgatgtg cgagcctcct
     4981 tcccgtcgca agatataagc gcacataaca agcccagagt aggaggaggc tagggatgga
     5041 tggttgggtg acgtttcaga cctggcggca gcacagcgag gatctccgac gcgaagcaga
     5101 gacccggcgg ctggccaggg agctgcgaaa aggtagtaca ggacgctccc gctggttccc
     5161 ggttctgggg tgggaactgc ggcggtacgg cggtagggtt ctcaagtcct tcggaggaag
     5221 acgcgcagag tgtagggacc attaggtcca tagtccggac aaaatgtgga ggaataggat
     5281 gaacgatgaa ccgttggcgc agatagagcg cgagatcttg ggatgggatg gagtcttcaa
     5341 gaagagggac gaggacggac caggtgggat cggcgtcacc ggctacaggt acggagatcc
     5401 cgagacggga gggcctcaga tagggcacgt acacgac
//
LOCUS       DASTKM010000129         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1088818, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000129 DASTKM010000000
VERSION     DASTKM010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088818"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFQ10_05610"
     CDS             complement(<1..472)
                     /locus_tag="VFQ10_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964851.1"
                     /translation="MTAGQSQRNFIVVHPDFDRTWPWAADHFRVLWEEQGQTEYLRLA
                     PDDRRVVCEIVADPAGVTRLVALNVPVTVECVARMPALREAVFSPEPSTEVGDALAAG
                     GVRNYVQPSEGFWGQSVAEFALALTLCGLRRIPQLHRAIVTSPQAWDYAPPGGIG"
     gene            607..1164
                     /locus_tag="VFQ10_05615"
     CDS             607..1164
                     /locus_tag="VFQ10_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9964852.1"
                     /translation="MGIVLIVIVVVVVILVIFAIVTYNGLVRRRNEVEESYRQIDVQL
                     KRRYDLIPNLLEGVKKYFRQEQDVLTQITQARSQAMQPQSPGQQAQSEARLSGAIGNL
                     FAVAENYPDLRSNTVLIDFQEELSSTENKISFARQHYNDSVQSYNTKIQSFPAVIFSR
                     AMGFTEREYFEAQGPERESVTVSYD"
     gene            1169..2155
                     /locus_tag="VFQ10_05620"
     CDS             1169..2155
                     /locus_tag="VFQ10_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET9964853.1"
                     /translation="MDQRDQGTFRERIARNRRNSLLLIAAFLAFVTIFGYIIGFAWLG
                     DPTRALFGLALALVVGVIAGLVSYFAGDKMVLAASRAREITHEDAPVLFNVVEEMSIA
                     SGLPMPKVYIVDDSAPNAFATGRDPEHATVAVTSGLLKKLDRDELQGVMAHEMSHVAN
                     FDIRYSMLVGILVGTTVLISDFFLRGLWFGGGRGGGRRGGDGGGQLQIIMILIAVVLA
                     ILAPLFARLLQLSISRQREFLADATSVRLTRNPKGLADALEKISGDREVLEVANRATA
                     HLYIVNPIKRFEKRSKGLFSTHPPIEERIKILRAMESGGVAETSGQTSTETP"
     gene            2181..2251
                     /locus_tag="VFQ10_05625"
     tRNA            2181..2251
                     /locus_tag="VFQ10_05625"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2213..2215,aa:Gln,seq:ctg)
BASE COUNT          451 a          723 c          664 g          420 t
ORIGIN      
        1 gcccgatgcc gccgggcgga gcgtagtccc atgcctgcgg gctggttacg atggcgcggt
       61 gcagttgcgg gatgcgacgc agaccgcaaa gagtgagcgc gagggcgaac tcggcgacgc
      121 tctgccccca gaagccctcg ctgggctgca cgtagttgcg tacgcccccg gccgccagcg
      181 cgtcgcctac ttcggtagac ggctcggggg agaagacagc ctcccgcaga gcaggcatcc
      241 gcgccacgca ctccaccgtc acgggtacgt tcagggcgac gaggcgggtg acgccggccg
      301 gatcggccac gatctcacaa acaacacgcc gatcgtccgg ggcgagccgc aagtactcgg
      361 tctgaccttg ctcttcccac aacacgcgga agtgatcagc agcccagggc cacgtccggt
      421 cgaagtcggg gtgaacgaca atgaagttac gctgcgattg cccggccgtc atgctcgcat
      481 tctaatgcgt tcgagttcgg agtatagagt taggaatttc gagttgtcta tcacgtagcc
      541 ccttccacct tcacaattcc tgtgacggga tataatgcct ctggcatcct agcgcggagg
      601 ttttggatgg gtattgtctt gatagtcatc gttgtggtcg tggtgatact ggtgatcttc
      661 gccatcgtca cctacaacgg gctcgtgcgg cgacgcaatg aggtagagga gtcttacagg
      721 cagatagatg tacagctcaa gcggcgatat gacctgatcc cgaacctcct cgaaggcgtg
      781 aagaagtact tccgccagga gcaagacgtg cttacgcaga taacgcaggc ccgctcccag
      841 gctatgcagc cccagtcgcc gggacagcag gcccagtccg aggcccgtct ttcgggggct
      901 attgggaacc tcttcgcggt cgctgagaat tatccggatc tcagatccaa cacggtactg
      961 atagacttcc aggaggagct ctcatcgacg gagaacaaga tctccttcgc aaggcagcac
     1021 tacaatgact cggtccagtc ctacaacact aagatacaga gcttccccgc ggtcattttc
     1081 tcccgtgcca tgggcttcac cgagagggaa tacttcgagg cccagggacc cgagcgggag
     1141 tccgtgaccg tctcctacga ctaggaccat ggatcagcgg gaccagggca ccttcaggga
     1201 gcgcatcgcc cgtaacaggc gcaacagcct gctgctcata gccgccttcc tcgccttcgt
     1261 caccatcttc ggctacataa tcgggtttgc ctggctcggt gaccccacac gcgccctctt
     1321 cggtctcgcc ctggccctgg tagtgggcgt gatcgccggg ctcgtcagtt acttcgcggg
     1381 cgacaagatg gtccttgccg catcgagggc cagggagatc acacacgagg acgcccccgt
     1441 actcttcaac gtggtcgaag agatgtccat agcctcgggc ctgccgatgc cgaaggtcta
     1501 catcgtcgac gacagcgccc cgaacgcctt cgccacgggc cgcgaccccg agcacgccac
     1561 cgtcgccgtc accagtggcc tcctcaaaaa gctcgaccgc gacgagctcc agggcgtcat
     1621 ggcgcacgag atgtcccacg tcgccaactt cgacatccgc tattccatgc tcgtcggtat
     1681 cctggtaggc accacggtct tgatctccga cttcttcctg cgcggcctgt ggttcggtgg
     1741 aggaagggga ggagggcgca ggggaggaga cggtggcgga cagttgcaga tcatcatgat
     1801 cctcatcgcc gtcgtcctcg ccatcctcgc gcccctgttc gcccgtctct tgcagctctc
     1861 cataagccgg cagcgcgagt tcctcgccga cgccacctcc gtcaggctaa cccgcaaccc
     1921 caaggggctc gccgacgctt tggagaagat cagtggcgac agggaagtcc tggaggtagc
     1981 caaccgcgcg accgcccacc tttacatcgt caaccccata aagaggttcg agaagcgttc
     2041 caagggcctt ttctccaccc acccgcctat agaggagagg atcaagatcc tacgcgccat
     2101 ggagtcaggc ggagtcgccg agaccagcgg acaaacctcg acggagaccc cataaggcgt
     2161 tacaatatcc gccgttgcct tgccggatgg tgtaacggca gcacgagtga ctctgaatca
     2221 cttagtcctg gttcgaatcc aggtccggca gcttctaa
//
LOCUS       DASTKM010000130        14500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1441533, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000130 DASTKM010000000
VERSION     DASTKM010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14500
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441533"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..932
                     /locus_tag="VFQ10_05630"
     CDS             105..932
                     /locus_tag="VFQ10_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET9964854.1"
                     /translation="MWTLTGFADEISPELEGQLETLGEESIGYMELRSVWNTNVLDLS
                     DDELDRVKSAAAQRGIGISSIGSPIGKVPVTEPFGRHLERFRRALHAADVMETPYIRV
                     FSFFIPEGHEPGHYREEVLDRMGIMAGEAEDSGVTLLHENEKEIYGDVPSRCVDILDG
                     VGSPALRAAWDAANFVQCGVSPYDEGYASLRPYVEYVHVKDALYGSDTVVPAGEGDGQ
                     LPETLSALRASGFHGFFSLEPHLASAGKYSGFSGPELFRKAAVAFKELLRRQDIEWA"
     gene            923..2431
                     /locus_tag="VFQ10_05635"
     CDS             923..2431
                     /locus_tag="VFQ10_05635"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HET9964855.1"
                     /translation="MGLTENTATTLLALNSLDVRPNSAVCVEGTEAGLVGSGQSVAVL
                     APSGSQVPSNFEGESSVAGGRTLVIGPMNAANLDALRDLLPWLRPRPLGPSTSAGFGD
                     RLGLATPGHVRALRAAGGELAPIFAQQSIREMERTGRSSQEVLDDATWGVFAEGWKEG
                     FGADADHLKTTEDVDACVAAGYTFFTFDPGEYVDDDAEAAGASALHKALEGLPWRDLE
                     DHPADMKRRYLDEAFEAEGHEVRFDDVSLARAAVKYGAAVAHVARLYRHLQAAMGEED
                     FEVEVSVDETESPTTHAQHFYAATELRRLGVRWVSLAPRYVGRFEKGVDYLGDVAGFE
                     EDIEVHAAIARTVSPDAPYKLSLHTGSDKFPVYPAFVRQTRGLAHLKTAGTSYLVALR
                     TVAALHPDLFREIYDFSRGRYEEDRATYHVSASLERAVQPRDMPDGELPALLERFDER
                     EILHVTFGSVLKEARFRDRLLGLVRDHPDHYAAGPEEHFLRHLEPFANGGCK"
     gene            2428..3870
                     /gene="uxaC"
                     /locus_tag="VFQ10_05640"
     CDS             2428..3870
                     /gene="uxaC"
                     /locus_tag="VFQ10_05640"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HET9964856.1"
                     /translation="MSKPWTLEPDRLFDPNPARRSLARSLYTEVEDLPIVGPHGHVPP
                     ALLADQDATLGTPAELFIIPDHYVFRMLYSQGVPMEDLGVPTVDGAPVETDHRRVWQR
                     FCENFHLFRATPTGLWLSDELINVFGVTERPTGENAQRIYDHLQELLASPEYAPRVLF
                     ETFGVEILCTTDAATDTLEHHATLRAEGWDGRVRPTFRPDAVTDLSAPGWHENIDLLS
                     QASGIDVIDYRSFLEALQEQRTFFKEMGAVATDHSATTPHTERLSKADAEGIFARAMS
                     GRAGGEDAARFTANVLVEMAAMSVEDGLVMQLHAGSLRNHNRAVHRRFGPDAGADIPI
                     AADWTRGLRVLLDEWGNDPRFRLVVFGLDEAAYSRELAPLAGHYPALLLGAPWWFLDS
                     PLGMRRYLDAVMETAGLYNTAGFNDDTRAFASIPSRHDVWRRITCDWLAGMVLTGVVD
                     EEEATELAREFAYGLAKRAYGFQREPVGRA"
     gene            3882..4721
                     /locus_tag="VFQ10_05645"
     CDS             3882..4721
                     /locus_tag="VFQ10_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9964857.1"
                     /translation="MEQRSMQDLFSLAGKVAVVTGATGVLGSEMARSLARSGARVAVL
                     GRREKKANRVAGEIEAAGGESLALPADVLDTGQLQDARDALIERWGVVDILVNAAGGN
                     VPEATLSDGVGFFELPEKALRQVMDLNFLGTLLPCQVFGAAMVEGAEEPEGCIVNVSS
                     MAASKILTNVVGYSAAKAAVDNFTRWLAVELARRYGPGLRVNAIAPGFFIGEQNRDLL
                     LDEDGSPSQRGQTIIQHTPAGRFGEPDELGGTLIWLCSQASAFVNGIVVPVDGGFSAF
                     SGV"
     gene            4968..5930
                     /locus_tag="VFQ10_05650"
     CDS             4968..5930
                     /locus_tag="VFQ10_05650"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET9964858.1"
                     /translation="MREFQGYTRERQGTRRISWWMWSLPVVLVVLFVGIELVAGPVSI
                     ERLTSASPPDPGKAEASPSNWVERMFGGQPGVTDGPLNVLVLGVDTRPDSEEMGSRTD
                     TIMLVQVVPKTGDVKLLSVPRDLLVEVEQGESDKINAAYNYGGVGQTIDALENYSGVP
                     VDHYAVVDFEGFERVIDAIGGVRVDVGDGQFPEKWRMGEGVQRLNGHKALIYARYRGT
                     PGADIDRMQRQRELVGALRSEALRWHTVKTLPQIMEVMNENVQTDLDLDGAITLGQVL
                     IRRGRHAEMTSQQIQGTPEILSNGEQVLIPDEEPNQAILDEFRH"
     gene            6161..6241
                     /locus_tag="VFQ10_05655"
     tRNA            6161..6241
                     /locus_tag="VFQ10_05655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6194..6196,aa:Leu,seq:tag)
     gene            6284..7663
                     /gene="tig"
                     /locus_tag="VFQ10_05660"
     CDS             6284..7663
                     /gene="tig"
                     /locus_tag="VFQ10_05660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET9964859.1"
                     /translation="MTANVTKLEENKVRLDIEVTPETVRQGVEAKIRELGQQVRVPGF
                     RPGKAPRRVIENRLGRDYIYMEALQEQLPGWYSQAVVETDVRPIDRPEIHFDDPLDEE
                     EGFKFSATVEVRPEAILGDYKGVEVPRREVEISEQDVEDRIEDMRAQFATLAAVEGRP
                     VQEGDFVILDFKGERLAGGPLEGAEAQDYLLEIGGGELLPDFEENIVGMNANERRQFG
                     VTFPMDYEEETLRGQSVLFNVHVKEIKERDLPPLDDEFVKEASEFETLDELRVAVREE
                     LEATAEQRVEGEFRARVLDVVAEGGDVEVPEVMVHEKAHEMVESFERSISSQGMDPEQ
                     YYELAGTDHAAFEERVMPDAEDTIRKELVLDAVATAEGIVADEDEVMHEIGHLAEDSE
                     RSAEEIAKTLRDNGTYALLEEEISRQKALDFLVENAVPVPMPEEETDEEPVEAKAGAT
                     ESEEREEQE"
     gene            7660..8253
                     /locus_tag="VFQ10_05665"
     CDS             7660..8253
                     /locus_tag="VFQ10_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET9964860.1"
                     /translation="MSIDNVIPYVIEQSPRGERAMDIYSRLLKDRIIFLGTPVDDQVA
                     NAIMAQLLHLESEDPEQDINLYINSPGGSVTAGLAIYDTMQFVKPDISTTALGMAASM
                     GAFLLAAGAKDKRNALPNTRILLHQPSVGGLAGQASDVEIHAKELIMTKRRLNEILAA
                     NTGQDYERIERDTDRDYIMGPEEGIEYGVIDNIVRNH"
     gene            8306..9565
                     /gene="clpX"
                     /locus_tag="VFQ10_05670"
     CDS             8306..9565
                     /gene="clpX"
                     /locus_tag="VFQ10_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET9964861.1"
                     /translation="MRDPSDQLQCSFCGKSQRQVRKLIAGPGVYICDECIELCNEIID
                     EEFSGPEVLKDDDLPKPREINRILNEYVIGQEDAKRVLAVAVYNHYKRIQMGAEATDG
                     TELQKSNILLVGPTGSGKTLLAQTLAKILNVPFAIADATALTEAGYVGEDVENILLKL
                     IQAADFDVKKAETGIIYIDEIDKVARKADNPSITRDVSGEGVQQALLKILEGTVASVP
                     PQGGRKHPHQDFLQIDTKNVLFICGGAFGGLEEIIRQRIGKKSIGFGSDIDSRLKQED
                     DSILQFALPEDLLKYGLIPEFVGRLPVISTLRSLEEADLVRILTEPKNALVRQYRQFF
                     RYDKVDLTFTDDALGAIAELALERGTGARGLRSILESALLPTMYELPSRSDVNKCVVD
                     VDTVKDGVQPSLVTGSQKYSYDEEETA"
     gene            9573..12125
                     /locus_tag="VFQ10_05675"
     CDS             9573..12125
                     /locus_tag="VFQ10_05675"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET9964862.1"
                     /translation="MSRAQIPKTYDHRAVEGRWYEEWERRGAFDADPNPEKEPYCIVL
                     PPSNVTGALHMGHALNGAIQDTLIRRARMKGYQTLWLPGVDHASIAVQNVVERKIMRE
                     EGKSRWDLGREAFVERCREFALKTREQILSQYKRLGASLDWRRLRYTMDEDYVDAVLT
                     AFIRLYNKGLIYRGNRITNWCPHDRSAISDLEVNYEDVEGKLYGIRYPFVDGEGPGPD
                     GEDYAQVFSTRPETMLGDVALVVNPEDKRYKDLIGRRVMVPFVEREIEVFADEHVDPE
                     FGTGVLKVTPAHDPNDFEIGGRLGLDPVNVMNPDGTINDAGGPFEGMSREEAREAVVK
                     ELDGLGLLGGVRPYAFRVGHCDRCGAVIEPWLSEQWWCSMRPLAEPSIEALKNREISV
                     YPDSWRRETIRWLENIRDWNVSRQLWWGQRIPVWYGPDGEVVASKESPGPDFEQDPDV
                     FDTWFSSSLWPIATLGWPDEDAEDLQYFYPTDLLSTAREIMYLWVARMIMMGLHFEDE
                     IPFQKVNVHSIVLAEDGTKMSRSKGNTIDPLELFDEYGTDAVRFGLLYQSSTQDFPYS
                     RERAEMGKAFVTKLWNAMRFVLAYPEPEEDGELSPADRWILSEYDRTVREYDAYLEEC
                     EFSEAMRRIYSFAWNQFADWYIEIAKAAPSSATPRVLREIFHGLLKLLHPAMPFATEE
                     MASVMGGQKLLATQSFPEPDPALQDEEADRLLDRTKRAVSAVRSFRAEAKIDGELEGL
                     VPQGIEEGVYFSLAGVRPAEAGDGASRASLPAGDVVVEILLSDEMRRGEIERLRREIS
                     HAEQEVERAQSKLANEKFVTNAPERVVAGEREKLDVNSRMLERLSRRLDEYI"
     gene            12144..13364
                     /locus_tag="VFQ10_05680"
     CDS             12144..13364
                     /locus_tag="VFQ10_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET9964863.1"
                     /translation="MTSFAQVCAELDRRRHITLGFERIEALLRAMGNPEREVPGLVQV
                     VGTNGKGTTALAIAAALEESGYPSGAYLSPHVLSYTERVVLRGGQVSEEDFAAAMGET
                     IELADRKGIPASQFELLTAGALGMFAAAGLSWAILEAGLGARHDATSVVRPDTVVLTN
                     VSIDHTEYLGETLEAIAEEKLASLRPGATLFLGTDEARIIDLAHSACLRVGAKLVLSG
                     EAGENTSFSGLAPYEADDVRLGIAVAESMLQMSLPPEVGQKVARAVGEALPGRFEVCE
                     VEGVPVVVDGGHNAAGLSAALAGMRAAYGDRPLAVVFGVLREKDAASMLAGLKGQANF
                     IVLTCPENDRAADPALLEQGLLDHKDGTALVVEDPVEALAVAVQAVREVEGVVLVTGS
                     LSTGAPVLRWLREA"
     gene            13461..13946
                     /locus_tag="VFQ10_05685"
     CDS             13461..13946
                     /locus_tag="VFQ10_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9964864.1"
                     /translation="MLALQQDPDPFQIISSFLESPILRISGQLVLLLFIVLWVSLVYW
                     TYADASRRGAMSVLWGFVAFIFPYVGTLVYLIVRPPEYLEESRERELELAVLERELTN
                     SVLLCPNCRNMVERDYLICPSCTWELKKSCVNCEKPLNMEWGMCPYCGTDQRSGKTIV
                     R"
     gene            13957..14358
                     /gene="ndk"
                     /locus_tag="VFQ10_05690"
     CDS             13957..14358
                     /gene="ndk"
                     /locus_tag="VFQ10_05690"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET9964865.1"
                     /translation="MEQSLVLVKGDGVRRKLVGEIIRRIEAKGLDIVDMKLMDVGREL
                     AELHYAEHSEKPFFEELVEFITATPVVAMRIRGEGAIKAMRTLMGSTNPADAAPGTIR
                     GDLALSMPDNLVHGSDSPESAERELGLFFGP"
BASE COUNT         2913 a         4410 c         4697 g         2480 t
ORIGIN      
        1 gggatctacg agtcggcccg tacaggcggg cccgtacgca tcgagtaata gaacaagctg
       61 gggcgggccg cgagagcacc gccccaggaa aggggttatg acacgtgtgg accttgacgg
      121 gtttcgcaga tgagatctcg cccgaactgg aggggcaact ggagacgctc ggggaggagt
      181 ccataggcta catggaactg cgcagtgtct ggaacacgaa cgtgctcgac ctgtcggacg
      241 atgagctgga cagggtaaag tctgcggccg cacagcgggg catcgggata tcttccatag
      301 gctcccccat aggcaaggtg cccgtaacag agccgttcgg gcggcacctg gagcgtttca
      361 ggcgcgccct gcacgccgcc gatgtcatgg agacgcctta catacgcgtt ttctccttct
      421 tcatccccga aggacatgag cctggccact atcgagaaga ggtgctcgac cggatgggga
      481 ttatggccgg cgaggcggaa gattccgggg tcacgctcct tcatgagaac gagaaggaga
      541 tctatggcga cgtcccgagc cgttgcgtgg acatactcga cggtgtaggc tcgcccgctc
      601 ttcgggcggc gtgggatgca gctaacttcg ttcagtgtgg cgtcagcccg tatgacgagg
      661 gatatgcttc gttgcgcccc tacgtagagt atgtgcacgt aaaggacgct ctgtacggct
      721 cggacacggt cgtaccggcg ggtgaaggag atgggcagtt gcccgagacc ctctccgcgc
      781 tacgcgcctc cggcttccac ggtttcttct cgctggagcc gcacctcgca tcagcgggaa
      841 agtactctgg tttcagcggc cccgagttgt tccgcaaggc cgcggtggcc ttcaaggagc
      901 tactccgcag gcaggacatc gaatgggcct gacggagaac acggctacca cgctgctggc
      961 cctgaacagc ctcgacgttc gcccaaactc ggccgtctgc gtagagggca cggaggccgg
     1021 cctggtgggg tcgggccaga gcgttgctgt ccttgccccg tcgggatctc aggtcccctc
     1081 caacttcgaa ggggagagca gcgtcgcggg cggccgtacg ctcgtgatcg gcccgatgaa
     1141 cgcggccaac ctggacgccc tgcgcgacct cctcccgtgg ctgcgcccgc ggccactcgg
     1201 accatccaca tctgcgggct tcggcgacag gctgggcctc gcaacccctg gccacgtcag
     1261 ggccctgcgc gccgctggcg gagagcttgc tcccatcttc gcccagcagt ccataagaga
     1321 gatggagcgt acgggcagga gctcccagga ggttctcgac gacgcgactt ggggggtctt
     1381 cgccgaaggc tggaaggagg gattcggggc tgacgccgac cacctcaaga ccactgaaga
     1441 cgtagacgcc tgcgtggccg caggttacac cttctttacg ttcgatcccg gagagtacgt
     1501 cgacgacgac gctgaagccg ccggcgcttc ggcgctgcac aaagcattag agggcctgcc
     1561 gtggcgggat ctggaagacc atccagccga tatgaagagg cgctacctcg acgaagcatt
     1621 cgaggctgaa gggcacgagg ttcgcttcga cgacgtctcc ctggcgaggg cagctgtcaa
     1681 gtacggcgcg gccgtggccc acgtggctcg tctttaccgc cacctccagg cggcgatggg
     1741 ggaggaggac ttcgaggtcg aggtctctgt ggacgagaca gagagcccca cgacccacgc
     1801 ccagcacttc tacgcagcaa cggagctacg gcgtctcggg gtcagatggg tgagcctggc
     1861 cccgcgctac gtcggacgct tcgagaaggg cgtggactac ctgggcgacg tcgcggggtt
     1921 cgaggaagac atcgaggtcc acgccgccat cgcccgcacc gtctcgcccg acgcgccgta
     1981 caagctgagc ctgcacacag gctcggacaa gttccctgtc taccccgcct tcgtcaggca
     2041 gaccagagga ctcgcccacc tcaagacggc gggcacgagc tacctggtgg ccctgcgcac
     2101 cgtggctgcg ctccaccccg acctgttccg tgagatctac gatttctccc gcggacgcta
     2161 cgaggaagac agggctactt accacgtttc ggcgagcctg gagcgggcgg tccagcccag
     2221 ggatatgccg gatggggagc ttccggccct gctggaacgt ttcgacgagc gtgagatcct
     2281 gcacgttacc ttcggatcgg tgctgaagga agcacgcttc cgcgaccgcc tcctggggct
     2341 ggtgcgcgac catcccgatc actacgccgc cggcccggaa gagcatttcc tgcgccacct
     2401 ggagcccttt gcgaatggag gatgcaagtg agcaagccct ggacgctcga acctgacagg
     2461 ctcttcgacc cgaacccggc caggcgttct ctggccagga gcctctacac agaggtcgag
     2521 gacctgccca tagtcgggcc gcacggccac gtgcccccgg cgctgctcgc agaccaggac
     2581 gccaccctgg ggacgcctgc cgagctgttt atcatccccg accactacgt cttcaggatg
     2641 ctctacagtc agggggtccc gatggaggac ctgggggtac cgacggtcga cggggcccca
     2701 gtggagacgg atcataggcg tgtctggcaa cgtttctgcg agaacttcca cctcttccgc
     2761 gccaccccga ccggtctttg gctctccgac gagctcatca acgtcttcgg tgtcaccgag
     2821 agacccacgg gagagaacgc gcagaggatc tacgaccatc tccaggaact gctggccagc
     2881 cccgagtacg cgccgcgggt cctcttcgag accttcggtg tggagatcct gtgtaccacg
     2941 gacgccgcga cagatacgct ggagcaccac gcgacgctgc gcgccgaggg ctgggacggc
     3001 agggtgcgac ccaccttcag gcccgatgcc gtgaccgacc tctctgctcc cggctggcac
     3061 gagaacatag acctcctctc gcaggcctct ggcatcgacg tcatcgacta ccgctccttc
     3121 ctggaagctc tccaggagca gcgaaccttc ttcaaggaga tgggggcggt agcgacggat
     3181 cacagcgcaa ccaccccgca caccgagcgc ctatcaaagg cggatgcgga gggaatcttc
     3241 gcccgtgcca tgtcgggacg cgccggcggt gaggacgccg ccaggttcac agccaacgtg
     3301 ctggtcgaga tggccgcaat gagcgtcgag gacgggctgg tgatgcagtt gcacgcgggc
     3361 agcctccgca accacaacag agcggtgcac aggcggttcg ggcctgacgc aggggctgac
     3421 atccccatag cggccgattg gacgcgcggc ctgcgggtcc ttctcgacga gtggggcaac
     3481 gacccgcgtt tccgcctcgt cgtgttcggc cttgacgagg ccgcgtactc cagggagctt
     3541 gcgcccctcg ccgggcacta tccggccctg ctcctaggag cgccgtggtg gttcttggac
     3601 agcccgctcg gcatgcggcg ctacctcgac gcggtgatgg agacggcggg cctctacaat
     3661 acagcgggct tcaacgacga cacccgcgca ttcgccagca ttccgtcccg ccacgacgta
     3721 tggaggcgca taacctgcga ctggctggca gggatggtcc tgaccggcgt tgtggacgaa
     3781 gaggaggcca ccgagttggc gcgcgagttc gcttacgggc tcgcaaagag ggcctatggg
     3841 ttccagagag agcccgtggg tagggcgtaa ggaggagtac agtggaacag agatcgatgc
     3901 aggatctctt ctccctcgcg ggaaaggtcg ccgtcgtcac aggcgcgaca ggggtcctcg
     3961 gctctgagat ggcccgctcc ctcgcgcgca gcggcgccag ggtggcggtg ctcggtcgtc
     4021 gcgagaagaa ggccaaccgg gtggcaggag agatagaagc agctggtggg gagtcactcg
     4081 cccttcccgc cgacgtgctc gacaccggcc agctccagga cgccagagac gccctgatcg
     4141 agcgctgggg cgtggtggat attctagtaa acgccgccgg cgggaacgtc cccgaggcca
     4201 ctctcagcga tggggtcggt ttcttcgagt tgccggagaa ggccctgcgg caggtgatgg
     4261 acctcaactt cctgggtacc cttctcccct gccaggtctt cggggcggcc atggtagagg
     4321 gtgctgagga gccagagggc tgcatcgtga acgtctcttc catggccgct tccaaaattc
     4381 tgaccaacgt ggtcggctac tcggccgcca aggccgccgt cgacaacttc acccgctggc
     4441 tcgccgtcga gctagcccgc cgatacggtc cgggtctcag ggtcaacgcc atagcgccgg
     4501 gcttctttat aggggagcag aacagggacc tgttgctcga cgaggacggt agcccgtctc
     4561 agcggggaca gaccataatc cagcacaccc cggcggggcg cttcggtgaa cccgatgagt
     4621 tgggagggac cctgatctgg ctctgcagcc aggcctctgc cttcgtcaac ggaatcgtcg
     4681 tgcctgtaga cggcggcttt tccgccttca gcggggtgta gatcagcatg tcagcacgcc
     4741 tgcttcgcag gcttgtcagc acttcagcat ttcagccagt cgtcccggtg cgtcaccacc
     4801 tgcgtcgaac ctgaccgtca ccaggagcgg ttcctcgtgc gccgggggcg aggcgggaaa
     4861 ggcactaact gaagtgctga caagtgaagt caaaggccgg agcgtgctga cttgctgaat
     4921 tgctgaaatg ttgctttgcc tgttgacgcg gattagactc cctctggatg agggagttcc
     4981 aaggttacac cagggaacgc cagggtacgc gccgcatcag ctggtggatg tggtcgttac
     5041 ctgtcgtgct agtcgttctc ttcgtaggca tcgagctcgt cgcgggcccg gtctccatcg
     5101 agaggcttac cagcgcgtcg ccgcctgacc ctggcaaggc ggaggcttcc ccgagtaact
     5161 gggtcgagag gatgttcggt ggacaaccag gggtcacaga cgggccgctc aacgtgctcg
     5221 tgcttggcgt ggacacgagg cctgacagcg aggagatggg gagccgcacg gataccatca
     5281 tgctcgtgca ggtggtgccg aagacaggag acgtcaagct gctctctgtt ccgcgggatc
     5341 tcctcgtcga ggtagagcag ggagaaagcg acaagataaa cgccgcctat aattacggcg
     5401 gtgtcggtca gacgatcgac gcgctggaga actactccgg cgtacccgta gaccattacg
     5461 ccgtagtgga tttcgaggga ttcgagagag tcatcgacgc gatcggcggg gtcagggttg
     5521 atgtaggaga cggccagttc cccgagaagt ggcgcatggg cgaaggcgtg cagaggctca
     5581 acggccacaa ggccctgatc tacgccaggt acagaggcac tcccggtgcc gacatcgacc
     5641 gcatgcagcg ccagcgggag ctcgtcggag ccctccgtag cgaggccctg aggtggcaca
     5701 ccgtcaagac attgccccag atcatggagg tcatgaacga gaacgtccag accgacctgg
     5761 atctcgacgg agccatcacc ctaggccagg tcctcatacg caggggaaga cacgccgaga
     5821 tgacctccca acaaatccag ggtaccccgg agatcctctc caacggagaa caagtcctca
     5881 taccggacga ggaaccaaat caggcaatcc tcgacgagtt ccgccattag agctagtccg
     5941 cacttcagct ttctcgtgcg caggtcccgg ctgacaagcg aaagccgaag gcttgagcgt
     6001 gctgaaatgc tgataagcgg aggccgcaag gccggagcgt gctgactagc gcgatttgct
     6061 ggaagcaaat cgccacgctt gcgcccatag cgtaaagggt cccttggcgg gcgggaagcg
     6121 attgggtggc tgctataatc cggcgcaggt cctacggcga gcgagagtgg tggaatggta
     6181 gacacgctag atttaggatc tagtgcctca cggcgtgcgg gttcaaatcc cgcctctcgc
     6241 acatcgccgc ccccgtcggg ccggtaaaga ggaactggag ggtatgacgg ctaacgtaac
     6301 gaaactcgag gagaacaagg tcaggctcga catcgaggtg accccagaga cggtcaggca
     6361 gggtgtcgag gccaagatca gggagctcgg gcagcaggtc agggttcccg gttttcgtcc
     6421 aggcaaggcg ccgcggcggg tgatcgagaa ccggctgggg cgcgactaca tctacatgga
     6481 ggccctccag gagcaactcc ctggctggta ctcgcaggcc gtcgtcgaga ccgacgtacg
     6541 cccaatagac cggccggaga tccacttcga cgacccgctg gacgaggagg aaggcttcaa
     6601 gttctcggcc accgtggagg tgcggcccga ggccatacta ggtgactaca agggcgtcga
     6661 agtcccgcgg cgcgaggtgg agatctcgga gcaggacgtc gaggacagga tcgaggacat
     6721 gcgggcccag ttcgccaccc tggccgccgt cgagggtagg ccggtccagg agggagattt
     6781 cgtgatcctg gacttcaagg gcgagaggct ggccggaggg ccgctggagg gcgccgaggc
     6841 ccaggattac ttgctcgaga tcggaggcgg cgagctcctg ccggatttcg aggagaacat
     6901 cgtggggatg aacgccaacg aacgcaggca gttcggcgtc accttcccca tggactacga
     6961 ggaggagaca ctcagaggcc agtctgtgct cttcaacgtg catgtcaagg agatcaaaga
     7021 gcgcgacctg ccacctctcg acgacgaatt cgtcaaggag gcgagcgaat tcgagaccct
     7081 ggacgagctt cgcgtcgccg taagagagga actggaggcc acagccgagc agcgtgtgga
     7141 aggtgagttc cgtgcgcgcg tgctcgacgt cgtcgccgaa ggtggcgacg tagaggtccc
     7201 cgaggtcatg gtgcacgaga aggctcacga gatggtcgag agtttcgagc ggagtattag
     7261 ttctcaggga atggaccctg agcagtacta cgagctggca ggaacagacc acgccgcctt
     7321 cgaggagcgc gtcatgcctg acgccgagga caccataagg aaggagctcg tgctcgacgc
     7381 cgtcgccacg gccgagggaa tagtggcgga cgaagacgag gtaatgcacg agataggcca
     7441 cctcgccgaa gactccgaaa gaagcgcaga agaaatagcc aagacccttc gcgacaacgg
     7501 gacatacgct ctgctggaag aggagatctc acgccagaag gccctcgatt tcctggttga
     7561 gaacgcggtg cccgtcccca tgccggagga agagacggat gaagaaccgg tagaggcaaa
     7621 ggccggcgct acagagagcg aagaacgaga ggaacaggaa tgagcataga caacgtcatc
     7681 ccctacgtta tagagcagag cccgcgtggg gagcgggcca tggacatcta ctcgcggctg
     7741 ctcaaggacc gcatcatctt cctcgggacc ccagtagatg accaggtcgc caacgccata
     7801 atggcgcagc tcttgcacct ggagagcgaa gatccagagc aggacatcaa cctctacatc
     7861 aattccccgg gtggcagcgt cacggcagga cttgcgatct acgacaccat gcagttcgtc
     7921 aagcccgaca tctccacgac ggccctcggg atggccgcct cgatgggggc cttcttgctc
     7981 gccgccggcg ccaaggacaa gaggaacgcc ctgccgaaca cgcgcatact cctgcaccag
     8041 ccgagcgtcg gcgggctggc cggccaggcc tctgacgtag agatccacgc caaagagctc
     8101 atcatgacca agcgccgcct gaacgagatc ctcgccgcca acacagggca ggattacgag
     8161 aggatcgagc gcgacaccga ccgcgactac ataatgggtc ccgaagaggg gatcgagtac
     8221 ggcgttatcg acaacatcgt caggaaccat tagaggttcc tggggccatt ggaatcggta
     8281 gagatgtcga ggaggatgta gaggcatgag ggatccgtcg gatcaattgc agtgctcctt
     8341 ctgcggtaag agccagcgtc aggtcaggaa gctcatcgct ggccctggcg tctacatctg
     8401 cgacgagtgc attgagctgt gcaacgagat catcgacgaa gaattctccg ggcccgaggt
     8461 cctcaaggac gacgacctcc ctaagccgcg ggagatcaac cgcatcctca acgagtacgt
     8521 gatcggccag gaggacgcaa aaagggtcct tgccgtcgcc gtctataacc actacaagcg
     8581 catccagatg ggcgccgaag ccacggacgg cacggaacta cagaagtcca acatactgct
     8641 tgtgggaccg acaggctcag gcaagacgct cctcgcccag acgctggcca agatcctcaa
     8701 tgtgcccttc gccatagcgg acgcaacggc ccttaccgag gccggctacg tcggcgagga
     8761 cgtagagaac atcctcctca agctcatcca ggccgccgac ttcgacgtga agaaagccga
     8821 gaccgggatc atctacatcg acgagatcga caaagtagcc cgcaaagcgg ataacccttc
     8881 gatcacgcgc gacgtctcgg gcgaaggcgt gcagcaagcc ctcctcaaga tcctggaggg
     8941 cacggtcgcc agcgtaccgc cgcagggggg gcgcaagcac ccgcatcagg acttcctgca
     9001 gatagacacc aagaacgtcc tgttcatctg cggcggcgcg tttgggggtc ttgaggagat
     9061 catccgccag cgcatcggca agaagagcat aggcttcggc agcgacatag actcgcgcct
     9121 caagcaagaa gacgactcca tcctgcagtt cgcgctcccc gaggacctcc tgaaatacgg
     9181 tctgatcccc gagttcgtcg gacgtctgcc cgtgatctca accctgcgca gcctggagga
     9241 ggctgacctc gtacgcatcc tcaccgagcc gaagaacgcc ctggtgaggc aatacaggca
     9301 attcttccgc tacgacaaag tggacctgac cttcacggat gatgccctcg gcgccatagc
     9361 ggagctcgca ctcgagcggg ggacgggggc ccgcggcctc aggagtatcc tcgagtcagc
     9421 ccttcttccc acgatgtacg agctcccgag ccgctcggac gtgaacaagt gcgtcgtcga
     9481 cgtcgatacg gtgaaggacg gtgttcagcc gtcacttgta acaggctccc agaagtactc
     9541 ctacgacgaa gaagagaccg cctgaaaact ccttgagcag agcacagata ccaaaaacct
     9601 acgatcaccg agccgtcgag ggccgctggt acgaagagtg ggagcgccgc ggagcgttcg
     9661 atgcagaccc gaaccccgag aaagagccat attgcatcgt cttgccgcct tcgaacgtta
     9721 cgggcgcgtt gcatatgggc cacgcgctaa acggtgccat tcaagatacc ctcatccgac
     9781 gcgccaggat gaagggctac cagaccctgt ggcttccagg cgtcgaccac gcctcgatag
     9841 cggtacagaa cgtcgtcgag cgcaagatca tgcgcgaaga gggcaagtcg cggtgggacc
     9901 tcgggcggga ggctttcgtc gaacgctgcc gcgagttcgc tctgaagacg agggagcaga
     9961 tactgagcca gtacaagcgg ctcggcgcct cactcgactg gcgtcgcctc aggtacacca
    10021 tggacgagga ttatgtcgac gctgtcctga cggcctttat ccgcttgtac aacaaaggtt
    10081 tgatctaccg cggcaaccgt atcaccaact ggtgccccca cgaccgttct gcgatcagtg
    10141 acttggaggt caattacgag gacgtggagg gcaagctcta cggcatccgc tatcccttcg
    10201 ttgacggcga aggccccgga cccgacggag aggattacgc acaggtcttc tctacccgtc
    10261 cggagacgat gctcggcgac gtggccctcg tcgtcaaccc tgaggataag aggtacaaag
    10321 atctcatcgg gcgcagggtc atggtgcctt tcgtcgagcg agagatagag gtcttcgccg
    10381 acgagcacgt agatcccgag ttcggtacgg gtgtcctcaa ggtcaccccg gcgcatgacc
    10441 ccaacgactt cgagataggt ggcaggcttg gcctggatcc tgtcaacgtc atgaatcctg
    10501 acgggacgat caacgacgct ggtggtcctt tcgagggtat gagccgcgag gaggcccgcg
    10561 aggcggtcgt gaaggaactg gacgggctgg ggctcctcgg tggtgtccgt ccttacgcct
    10621 tcagggtcgg gcattgcgac aggtgcggag cggtcatcga gccgtggcta tcggagcagt
    10681 ggtggtgctc gatgaggccg ctcgccgaac catccatcga ggctctgaaa aaccgtgaga
    10741 tctcggtcta tcccgactcc tggcggcggg agaccatcag gtggctggag aacatccgcg
    10801 actggaacgt ctccagacag ctctggtggg ggcagcgcat tccggtgtgg tacggccctg
    10861 acggtgaagt agtagcttcc aaagagtcgc cgggccctga tttcgagcaa gatcccgacg
    10921 tgttcgacac ctggttctcc agcagcctgt ggcccatcgc gaccctggga tggcccgatg
    10981 aagacgcgga agacctgcag tacttctacc cgacagacct cctctcgacc gcgcgtgaga
    11041 tcatgtacct ctgggtggcc cgcatgatca tgatgggcct ccatttcgaa gacgagatcc
    11101 ccttccagaa ggtgaacgtc cactctatcg tgctcgccga ggacgggacg aagatgagcc
    11161 gctccaaggg aaacaccata gatcccctgg agctattcga cgagtacggt accgacgccg
    11221 tgcgcttcgg cctcctctac cagtcctcga cgcaggactt cccgtactcg cgcgagcggg
    11281 ccgagatggg caaagcgttc gtcacaaagc tatggaacgc catgcgcttc gtgctcgcct
    11341 accccgagcc tgaagaagat ggagagctct cacctgctga ccgttggatc ctctctgagt
    11401 acgatcgcac ggtgagggag tacgacgcct atctggagga gtgcgagttc tctgaggcca
    11461 tgcgccggat ctactctttc gcctggaacc agttcgccga ctggtacatc gagatagcga
    11521 aggcagcccc ctcctcagcg acgccccgcg tcctgcgcga gatcttccac ggtctcctga
    11581 agctcctgca ccccgctatg cccttcgcga cggaggaaat ggcaagcgta atgggagggc
    11641 aaaaactgct cgcgacgcag agcttccccg agcctgaccc cgcgctccag gacgaggagg
    11701 ccgaccggct gctcgaccgc accaagcggg ccgtctcggc ggttcgctcg ttcagggcgg
    11761 aggcgaagat cgacggcgag ctcgaaggcc tcgtgccaca gggcatcgag gaaggcgtct
    11821 acttctcgct agccggggtg agacccgcag aggccggaga cggcgcgagc cgggcttcgc
    11881 tgccggccgg ggacgtagtc gtggagatcc tgctctccga tgagatgcgc cgcggcgaga
    11941 tagagcgact taggcgggag atctcacacg ccgagcagga agtggagcgc gcacagagca
    12001 agctcgccaa cgagaaattc gtgaccaatg cgccggagag ggtcgtcgcc ggcgagcgtg
    12061 agaagctgga tgtcaactcc aggatgctcg agaggctctc tcgccgtctc gatgaataca
    12121 tttaggagcc ggccattcct actatgacct ctttcgccca ggtctgtgcc gagttggacc
    12181 ggcgccgtca catcaccctc ggtttcgagc gcatagaagc cctcctcaga gccatgggca
    12241 atcctgaacg cgaagtgccc ggcctcgtgc aggtcgtcgg cacgaacggc aagggcacaa
    12301 cggccctcgc catcgcagcg gcccttgagg agtcgggata tccgtcaggc gcttacctct
    12361 cgccgcacgt cctctcctac accgaacggg tcgtacttcg cggcggccag gtctcggagg
    12421 aggatttcgc agccgctatg ggagagacca tagagctcgc ggatagaaag ggcatccccg
    12481 cctcgcagtt cgagctcctt accgcaggag cgctcgggat gttcgcagcg gcggggctgt
    12541 cgtgggccat cctcgaggca ggcctcggcg cgcgccacga cgccacctcc gtcgtcaggc
    12601 ccgacaccgt ggtgctcacc aacgtctcca tcgaccacac cgagtacctg ggtgagaccc
    12661 tcgaagcgat agccgaagag aagctcgcca gtctgaggcc gggcgcgacc ctgtttctcg
    12721 gtactgacga agcgcgaatc atcgaccttg cgcacagcgc atgcctccgg gttggggcga
    12781 agttggttct gtccggcgaa gccggcgaga atacctcatt ttcgggcctc gcaccgtacg
    12841 aggcagacga cgtgcgcctg gggattgcgg tggccgagtc aatgctgcag atgagtctcc
    12901 cccccgaggt cggacagaag gtcgcgcggg ctgtcgggga ggcacttccc ggacggttcg
    12961 aggtatgcga ggtcgagggc gtccccgtgg tggtcgacgg tgggcacaat gcggccgggc
    13021 tctcggcggc gctcgccggg atgcgcgccg cgtatggaga caggcccctg gcggtggtct
    13081 tcggcgtgtt gcgagagaag gacgccgcaa gtatgcttgc cggattgaaa ggtcaagcca
    13141 actttatcgt gctgacgtgc cctgagaacg acagggccgc cgaccccgca ctcctcgagc
    13201 agggtctcct cgaccataaa gatgggacag ccctggtagt cgaggaccct gtagaggccc
    13261 tcgctgtggc cgtgcaggca gtaagagaag tggagggagt tgtgctcgtt acggggtccc
    13321 tctccacagg ggcaccggta ttgcggtggt tgcgtgaagc ataagggcgc cttgcagctc
    13381 atcggttttc tcgtggtggt cgccgtggtc tcctttgtgg caggttactt cgtgatggta
    13441 aggtttatcg gatgagatgc ttgctcgcac ttcaacagga ccctgacccg ttccagatca
    13501 tctccagctt tttggagagc ccgatcctga ggatctcggg ccaactcgtg cttcttctat
    13561 tcatcgtgtt atgggtatcg ctcgtctact ggacctacgc cgatgcctcc aggcgggggg
    13621 ccatgagcgt gttgtggggg tttgtcgcct tcatcttccc ctacgtcggc accctcgtct
    13681 acctcatcgt acgcccacct gagtacctcg aagagtcccg cgaacgcgag ctggagctgg
    13741 ccgtgctgga gcgcgagctc acgaacagcg tccttctgtg ccccaactgc cgcaacatgg
    13801 tcgagaggga ctacctgatc tgcccttcgt gcacctggga gctcaagaag tcttgcgtaa
    13861 actgcgaaaa accgctcaac atggagtggg ggatgtgccc ttactgcggc accgaccagc
    13921 gcagcggcaa gacaatcgtt agataaggag agaagaatgg aacagtcgct ggtattggtc
    13981 aagggtgacg gcgtgcgccg caagctcgtc ggtgagatca tccgccgcat tgaagccaag
    14041 ggcctcgata tcgtggacat gaagttgatg gacgtgggca gggaactggc agaattacac
    14101 tatgccgagc acagcgagaa gccgttcttc gaagagctgg tcgagttcat caccgcgaca
    14161 ccggtcgtcg ccatgaggat aaggggcgaa ggcgcgataa aggccatgag gaccctgatg
    14221 gggtccacga accctgccga tgcagcgcct ggtacgatcc ggggcgacct ggccctaagc
    14281 atgcctgata acctcgtcca cggctctgat tcgcccgaga gcgcagagcg ggagctgggc
    14341 ctcttcttcg gaccttgaaa ggcttgcgcc tcgtactcgc ttcggagtcg gccaggcggg
    14401 tggatctgct ggataccgca ggctacgtgt tcgaggctca taagagcggc ttccccgagc
    14461 tgacgctcga agaccccgta gagaccgtta gggccaacgc
//
LOCUS       DASTKM010000131         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_913608, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000131 DASTKM010000000
VERSION     DASTKM010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913608"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFQ10_05695"
     CDS             complement(<1..521)
                     /locus_tag="VFQ10_05695"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9964866.1"
                     /translation="MELRQVRTFRVVAEELSFSRAAAKLGYVQSSVSAQVAALERELG
                     VRLFDRLGRRISLTEAGSVMLAYSRELLVLAGEAREAVVDAGVGAGEVTGSLTVSAPE
                     TLLTYRLPRLLAIFHERHPKVRLSVHPSAIGRLVGSMRRAVDEGRVDVAFVLDGPVGA
                     PGLSVEPLIGEGVT"
     gene            646..1887
                     /locus_tag="VFQ10_05700"
     CDS             646..1887
                     /locus_tag="VFQ10_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9964867.1"
                     /translation="MAREEGGVRGGLRSGLPAVVLLLMGTLGISTAFGMLLLLPLYVQ
                     ELGGDEADFGLVLSAAAVPAVLCIGLLIRYPEALRPHVVVALAIATYGIGAVGASLVT
                     EGWRPLVGIGVLLGTAWAVVYAASPMAMSRMVTDEGRAAYFGYLTGTQQVGIGVGPVI
                     SRFLVETPLGFRGTFLMAGVVCLIAVALTLVVGALVSDAGTDAGKEGAEGNGISQVSF
                     GAAMRRILMSEAAFSLAMILLFACLFTTMTSFQPTFAGARGLDYSVFYVAYTVAVIFS
                     RFVLAGVVARFDSRLVIAGSVSVMALAVASFLAVGSNGVFYGISSAFLGLGYGLALPG
                     VQAQAVNVSEEQVRPRVLPMAGMLFEGAILGFPLVAGWVITGFGYRVLFAVLLAFALV
                     QVAIAWWRFLVSGRAAKTQAA"
     gene            2058..2324
                     /locus_tag="VFQ10_05705"
     CDS             2058..2324
                     /locus_tag="VFQ10_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964868.1"
                     /translation="MKRMIKVLMVAVLMAVILAASVSPALAAKARGGVLLSTTQPCHA
                     IAQDASGEDLVLNPPGRAPGCWFLLPPGAASNPPIAIDSGGVVL"
     gene            complement(2385..>2833)
                     /locus_tag="VFQ10_05710"
     CDS             complement(2385..>2833)
                     /locus_tag="VFQ10_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964869.1"
                     /translation="ATELNEEAAELYRGRGRKGVLQVALNNLGWSALIRGDHRKAEAL
                     HEECLVLCRELGDKLIGAESIEGFACAAGAGGAAERAARLFGAAEALREAAGNQQAAR
                     AHALREPYLAAARARVDEATWSAAWEKGRTMEFEGAVVYALEDTDG"
BASE COUNT          448 a          906 c          889 g          590 t
ORIGIN      
        1 gtcacaccct ccccgatcag gggttctacc gagagcccag gagcaccaac gggtccgtcg
       61 aggacgaatg caacgtccac cctgccttca tcgacagccc ggcgcatgct acccaccaac
      121 cggccgatgg cgctcgggtg caccgagagc ctcaccttgg gatgtctctc atggaagatg
      181 gccagaagcc tcggcaaccg gtaagtgagg agcgtctcgg gggcgctcac cgtgagagag
      241 cccgtcactt ctcccgcgcc cacgccagcg tcgacaaccg cttcccgcgc ctcccctgcg
      301 aggaccagca actcccggga gtacgcgagc atcacagatc ccgcctcggt gagcgagatc
      361 ctcctgccca accggtcgaa caaccgcacc ccgagctccc gctccagcgc agccacctga
      421 gcgctcacac tcgattgcac gtagcccaac ttcgccgccg cccgcgagaa agaaagctcc
      481 tccgccacca ccctgaaagt ccggacctgc ctgagctcca cagaataccc ttcacttccg
      541 attcatcgaa tccatcgata cttagcatcg ttagatttcg ttggacacga tattagcacg
      601 gcactaggat cttctcgaac gacgcggatt acagatggag gagaagtggc gcgagaggaa
      661 ggcggggtaa gggggggctt gcggagtggc ctccctgccg tggtgctgtt gctgatgggg
      721 acgctgggga tctcgacggc cttcgggatg ctcctgctct tgcccttgta cgtgcaagag
      781 cttgggggcg acgaggcgga cttcggcttg gtcctgtctg cggcggcggt gcctgcggtc
      841 ctctgcatag ggctcctgat ccgctatccc gaggccttga ggcctcacgt cgtggtggcg
      901 ctcgctatag cgacttacgg gatcggggcc gtcggcgcat cgctcgttac cgaaggctgg
      961 aggccgctcg tcgggatcgg ggttctgctc ggcactgcct gggctgtggt ctacgcggcc
     1021 tcgccgatgg ccatgagccg gatggtaacc gacgagggga gggcagctta cttcggctac
     1081 ctcacaggca cccagcaggt cggtataggt gtcggacctg tgatctcccg tttcctggtg
     1141 gagacgcctc tgggcttccg gggtacgttc ctcatggcag gcgtcgtctg cctcatcgcc
     1201 gtggcgctta cgctcgtcgt cggggcgctc gtctctgacg ccgggacgga tgctggaaag
     1261 gagggtgcag aaggaaacgg aatatcacag gtctcattcg gcgcggcgat gcggcgtatt
     1321 ttgatgtccg aggccgcctt ctcgctcgcc atgatcctgc ttttcgcttg cctctttacc
     1381 acgatgacga gctttcaacc gaccttcgcc ggtgccaggg gtctcgacta ttctgtcttc
     1441 tacgtcgcct acaccgttgc ggtgatcttc tcccgcttcg tgcttgcggg ggtggtggcg
     1501 cgattcgatt cgcggctcgt gatcgctggg tcggtgagcg ttatggcgct tgctgtcgca
     1561 tcgtttctcg cggtggggtc caacggcgtc ttctacggga tctcctcggc cttcttgggt
     1621 ctgggttacg gtctcgcgct gccgggcgtg caggcgcagg cggtcaacgt ctcggaggag
     1681 caggtaaggc cgcgggtgct tccgatggcg ggcatgctct tcgaaggtgc gatcctcggc
     1741 ttcccgctgg tcgccggatg ggtgatcaca ggcttcgggt atcgggtctt gttcgcggtc
     1801 ctcctcgcgt tcgcgctggt acaggtcgcc atcgcctggt ggcgtttcct cgtctccgga
     1861 agggccgcca agacgcaagc agcgtaggaa ccccgccctc tgatcttcgt cttaatgttg
     1921 aacgggttgc tccggtatga tcggccagaa agttgccgaa agaagtaata gctcacttgt
     1981 acgataccgt cgtctgcttc ggtctggtat cgtgagtgcg tagagggcag aaaggtaagg
     2041 aacaaggagg ttggtcgatg aaacggatga tcaaggtctt gatggtggcg gtacttatgg
     2101 cggtgatttt ggcggcaagc gtctcccccg cgctggccgc gaaagcacga ggcggagtgc
     2161 tgttgtcgac gactcagccc tgccacgcga tcgcccagga tgcgtccggg gaggacctgg
     2221 tcctcaaccc acccggtcgg gcaccgggat gctggtttct gttgccgcct ggtgccgcca
     2281 gtaacccgcc aatagcgatc gactcggggg gtgtcgtact ctgagtgcta catccgttcg
     2341 ggtgctgagc gggagctctc tgggaggctg agccggcaga cagatcagcc gtcggtatcc
     2401 tccaaagcgt agaccacagc gccttcgaac tccatggtac gtcccttctc ccacgccgcg
     2461 gaccaggtcg cctcgtctac ccgagcgcgg gcggctgcca ggtacggctc tcgtagggcg
     2521 tgggctctgg ctgcttgctg gttacctgcc gcctcgcgca acgcctccgc ggccccgaac
     2581 agcctcgctg cccgctcggc tgctcctccg gctccggcgg cacatgcgaa cccctctatg
     2641 ctctctgctc cgatcagctt atcgcccagt tctctgcaca acactaggca ctcttcgtgc
     2701 aaggcctcgg ctttccggtg atcacccctg atcagcgccg accaacccag gttgttgaga
     2761 gcaacctgga gaaccccctt gcgtccccgc ccccggtaca actccgcagc ttcctcgttt
     2821 agctctgtgg ctc
//
LOCUS       DASTKM010000132         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_737824, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000132 DASTKM010000000
VERSION     DASTKM010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737824"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFQ10_05715"
     CDS             <1..313
                     /locus_tag="VFQ10_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964870.1"
                     /translation="PTWRSVGDGRLHLRGSPDNRPGLPAPRPARGWTREELAFLSWSR
                     YTGVIPAAVASLLLARGVEGAEIAVSLVALAVCATLLLQATTAGYVARRLGLIEEPGE
                     S"
     gene            590..1042
                     /locus_tag="VFQ10_05720"
     CDS             590..1042
                     /locus_tag="VFQ10_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9964871.1"
                     /translation="MSIFPTTILLATDGSTEAELARTTAIDLAESTNSELHVVTVAPG
                     YPSYDVLNPLVVEQLREQAEDILNEQAAKIEQEGGQVAEKHLRIAERYRAQQIVRVAE
                     DIGSGLIVMGSRGLGGVRRALMGSASDSVVRHAHCPVLVVRPEKEQEA"
     gene            complement(1173..1790)
                     /locus_tag="VFQ10_05725"
     CDS             complement(1173..1790)
                     /locus_tag="VFQ10_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964872.1"
                     /translation="MEHWHGFETARSIVQIHETTIADVASDLSAHFVTVPARSLGGSS
                     YQLQGSEGPLAALGLLPIADVPFGGVARSLAACLVSDDVETFVRKFQQGFEEETSHSS
                     VQLPEPFMFAEYLTFARVVPFEQPSLSADSLGNILTAQGHGEAGYMYHEETRTPLLAV
                     AIPAGIVICGSAPGMIRVLESGLRRRLVELVKSRSGEHKDGDAIS"
     gene            1916..2176
                     /locus_tag="VFQ10_05730"
     CDS             1916..2176
                     /locus_tag="VFQ10_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964873.1"
                     /translation="MQQRWALLGFLTGRGQLEELRAQFFRAGHAHAGVLLVLSLVYFL
                     YLDRTEYSTAVQWLAGLLLAAALIILAVGLLQEMSTQEEGSQ"
     gene            2187..2642
                     /locus_tag="VFQ10_05735"
     CDS             2187..2642
                     /locus_tag="VFQ10_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964874.1"
                     /translation="MSPQSRLVAGIILIIVPTVEIGGASILSLLIADPSYGQNDLRQD
                     LWRAGHAHAGVWLVLSLVALRYVDEASLSERMRWVVRLAFPAAAILMPLAFFLSVLSP
                     EATEPNALINLAFVAGLVLAVGLLVLGIGLIRGRHDPEQLTGAGATEGR"
BASE COUNT          530 a          818 c          853 g          535 t
ORIGIN      
        1 acctacttgg cggtctgttg gtgatggccg tcttcatctt cgtggctcgc ccgataaccg
       61 tcctggcctg cctgctccca gaccggcgcg ggggtggacg cgagaggaac ttgccttcct
      121 cagctggtcc cgctacacgg gtgtcatccc agcggccgta gcaagcttgc tgctggcccg
      181 cggggtcgag ggggcggaga tagcggtgtc cttggtcgcc ttggcggtgt gtgcgacctt
      241 gctgttgcag gccaccaccg cagggtatgt ggcccggcgt ctcgggctaa tcgaagagcc
      301 tggtgaatcg tagcgatgga acgtcggaga atgcgacttc cccgacgttg ggtgaatagc
      361 aactggctac gcttcttggg cacccacagc cgcgccttgc tattgaagta gcatcctctc
      421 cttgatacta tcgacacacg ggcgatggat cccgccctcc gctctcttag aaagagtagc
      481 gggcaaccgg tcgcactaag cggcctgatg gctcctatca cgctgtgtgt agcagcgcgg
      541 tgaggggtct ctcgcatctc ctcaccgcca aagaaaagga ggcgaggaga tgagcatctt
      601 cccgaccacg atcctgctgg ccaccgacgg ctccacggaa gcggagctgg cccgcaccac
      661 cgccatcgat ctagctgaga gcaccaactc ggagctgcac gtggtgacgg ttgcgccagg
      721 ttatccctcc tacgacgtcc tcaacccctt agtggtcgaa caacttcggg agcaagcgga
      781 ggacatcctc aacgagcagg ctgcgaagat cgagcaggag ggaggtcagg tcgccgagaa
      841 gcatctcagg atagccgagc gctatcgtgc ccagcagatc gtaagggttg ccgaggacat
      901 aggttcgggc ttgatagtga tggggagtag gggactcggg ggagtgagac gagcgctgat
      961 gggcagcgca tctgactcgg ttgtgagaca tgcccactgc cccgttttgg tggtccgtcc
     1021 tgagaaggaa caggaggcgt agagtagccc gaacttctta gacctccgtc atggcgacct
     1081 tcgcagaagg ccccagcctg ttgaaaaact cctctatgcc ccttccgagc acccttcaag
     1141 gactcaaaaa ccccgccatt gtaatccggt atttaggaga tcgcgtctcc gtccttgtgt
     1201 tcgccggagc ggcttttcac gagctccacg aggcgtcgtc tgaggcccga ctcgaggact
     1261 ctaatcatcc ccggcgccga accgcagatc acaatgccgg ccgggatcgc gacggccaag
     1321 agcggggttc tcgtctcttc atggtacata tagcctgcct cgccgtggcc ctgtgccgtc
     1381 aggatattgc ccagggaatc ggccgagagt gaaggctgct cgaacggaac gaccctggcg
     1441 aacgtgaggt actcggcgaa catgaagggc tcgggtagtt gcacgctgga gtggctggtc
     1501 tcttcttcga agccttgctg aaactttcgc acaaacgtct cgacgtcatc ggagaccaag
     1561 cacgcggcca ggctgcgagc tacgccgccg aacggcacgt ccgcgatcgg caacagcccc
     1621 aacgctgcca aaggcccttc cgacccctga agctggtaag agctgcctcc gagcgaacgc
     1681 gccgggaccg tgacgaagtg ggcggagagg tccgacgcga cgtcagcaat cgtcgtctcg
     1741 tgtatctgca caatgctgcg ggccgtctcg aaaccgtgcc agtgttccat actcgctgat
     1801 tatctcttat ccgcgtgcca cacgctggcg gcatatctca acgctgtatc aggggcatcc
     1861 attcggcatt cgacatgtgc caagatagca ggcagaatga tagaagggtg agaatgtgca
     1921 gcagagatgg gcactactgg ggttcctcac cggacgcggc cagcttgagg aattgcgtgc
     1981 gcaattcttc agggctggcc acgctcacgc cggagtcttg ttggtgctat cgcttgtcta
     2041 cttcctctac ctcgacagga ctgagtactc gaccgcggtc cagtggctcg cgggcctgct
     2101 ccttgcggcg gcgttgatca tcttggccgt aggcctgctc caggagatgt ccacgcaaga
     2161 agaaggatcg cagtgaaagg agcaatatga gccctcaatc gcgcctggta gctggcatca
     2221 tcctgatcat cgtccccacc gtcgagatcg gcggagcaag catcctctcg ctgctcatag
     2281 cagaccccag ctacggccag aacgatctca ggcaggacct ctggcgagca ggacatgccc
     2341 atgccggcgt gtggctcgtc ctctctctgg tagccctgcg ctacgtggac gaggcgtctc
     2401 tctcagagag gatgaggtgg gtggtgcgtc tggccttccc ggccgcagcg atactgatgc
     2461 ctttagcctt cttcctctct gtcctctccc ctgaggcaac cgagcccaac gcgctcatca
     2521 acctggcttt cgtggccggg ctcgtgctgg ctgtgggact gttagtattg ggtatcggcc
     2581 tcatccgggg gcgccatgat ccagagcagc tgaccggggc aggagctacc gaaggtcgat
     2641 agggaaccgg aacaagggaa aggagatgtc gatgaagatg cgggcgacga ggcgagcgaa
     2701 gcgatcctcc tctagaacgc agacggcgtt cgggcg
//
LOCUS       DASTKM010000133         8687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1442919, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000133 DASTKM010000000
VERSION     DASTKM010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442919"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFQ10_05740"
     CDS             <1..477
                     /locus_tag="VFQ10_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964875.1"
                     /translation="MKALEERGFYRRERRSFLGIIPYQRWALSRSGEAAREQLKLTMR
                     LGESQFGRWVDREPNRALAYLGLVGPAVLLMEPLHPDLQRLREVQDRTGDGVGSSGTS
                     GDSQGEESSGEDAPDSGDFDVDFDVLGDLGGAFDAIDSGMDAGGGDGGDGGGNGGD"
     gene            530..1441
                     /locus_tag="VFQ10_05745"
     CDS             530..1441
                     /locus_tag="VFQ10_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET9964876.1"
                     /translation="MKIYRSILAVPANNPRMVENALASAADAVFLDLEDAVAPDSKAE
                     ARKDIVCALKELEWRGRPTVFRANALDTPYFYRDLMEVVEGAGDSLDAIMIPKVNRPE
                     DLCAVSILLSQLELAMDLEKGRIEIEAQIESAEGLANVDSIARATGRLTTLHFGPGDF
                     AASVSMPQISIGVTDEWDEAYPGHRFHYAMQRIVVAARAAGLRVLDGPVADYGDEEGL
                     RRSCLIARSLGFDGKWCIHPAQIAVVNETFSPTDKEVDWARKVVAAYDEANAEGSGSV
                     SVDGQMVDAASIKLARNTLGKVPKKDT"
     gene            1522..2274
                     /locus_tag="VFQ10_05750"
     CDS             1522..2274
                     /locus_tag="VFQ10_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET9964877.1"
                     /translation="MNGPEQLPYVAQPAGLSARTVRWLRVLLLAAVAAVFSLLYLLSA
                     EFRSEMNRALGILGRGDIAGLRDYIVSFGLWAPVASCFLMVLQALVAPVPSFLITFAN
                     GLAFGVFWGWMLSLFGHVLAAAVCFGISRALGRVPVEVLVGKTGLESADRWFARWGVY
                     AVFAGRLLPGVAFDAISYAAGLTRMRFRSFIVATALGIFPQTFLYSYLGRQAPEYVAL
                     FLVTSGLVVLGVVMVAVIRYRGERRKPKRNLR"
     gene            2321..2953
                     /locus_tag="VFQ10_05755"
     CDS             2321..2953
                     /locus_tag="VFQ10_05755"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET9964878.1"
                     /translation="MTQIFDPPFDASTHRTGSLIVIMGSMFSGKTEELIRRVRRTLYA
                     HRSAQVFKHTIDTRSENSEIRSHNGVLHTALAVSTSEELLRKVEETTDVVAIEEAQFF
                     DEGIVEVCRRLADAGYEVIAAGLDMDFKGEPFGPMPRLLAEADEVTKLRAICARCGRE
                     ASRSQRLIDGRPALVSAPTILIGAEESYEARCRHCHEVPEGVFQYRFPGI"
     gene            2974..3405
                     /locus_tag="VFQ10_05760"
     CDS             2974..3405
                     /locus_tag="VFQ10_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964879.1"
                     /translation="MPRKYIVVLILFCITLNAAGFVWKLFEPVLFYDEVAHFITPFTL
                     VLILAEIIYRTGGDDEFFNTPMRAIITGSVIGLLGATGWEGIEVLMSWMGISVSNALL
                     DTIFDIFLGVLGGAAGAWAADRYLDRIFGRSRTNAHQPRVR"
     gene            3609..4199
                     /locus_tag="VFQ10_05765"
     CDS             3609..4199
                     /locus_tag="VFQ10_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964880.1"
                     /translation="MGTTALIVIIVVVVVVVLLLVLYFASRRRAAQRSEEQRERSRAE
                     FGSEYERTAQEIGSEEEAERELRERRGRVERRVEPLSEESRGRYEEQWGEVERVFVDN
                     PERSIEMADRTVSDILNERNFVSDAGQSDEETERGLAAMHPEVADDYREARRIRADVV
                     ARSAGRAEEDSNEESTEELRQAIRKYRAVYQRLVRE"
     gene            4221..4952
                     /locus_tag="VFQ10_05770"
     CDS             4221..4952
                     /locus_tag="VFQ10_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HET9964881.1"
                     /translation="MGALAAGFWGFVGGAALLVGALIGIYAGASRRTIATVMALGAGV
                     LISSVAFELMDEAYQRGGFDASALGLLLGAVAFFFADVAVNRRGGAHRKNPGDKQGES
                     ASAIAIGALMDGIPESAAIGISLLEGGNISAALVAAVFLSNVPEGLSSASGMKQAGRP
                     AAYIFALWSGVTVASALAALLGYFFLASASEDVVATIQAFAAGAILTMLASTMMPEAY
                     EEGGSVVGLATTAGFLLSFVLSHLE"
     gene            5107..5313
                     /locus_tag="VFQ10_05775"
     CDS             5107..5313
                     /locus_tag="VFQ10_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964882.1"
                     /translation="MASLVCPCARGADLWGEFWWVGREYKWVFFDDDNTSETFTEQVT
                     HCSGCGTPLDRKTLKVTSPIVRHQ"
     gene            5500..7482
                     /locus_tag="VFQ10_05780"
     CDS             5500..7482
                     /locus_tag="VFQ10_05780"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET9964883.1"
                     /translation="MAKNLVTKILEDHMVEGSLDPGEEVGLRIDQTLLQDATGTMACL
                     QFERMGVDRVQVDLAVQYVDHNVIQLDFKNPDDHRFLQGFAAKYGIHYSRPGNGICHY
                     LHVERFARPGATLVGADSHTTSSGALGSIAIGAGGLDVALVMAGQPFQSPAPKVVGVE
                     LTGALSDWVTPKDVILELLKRRGVRGGLGRIFEFYGSGVANIDVTGRTTICNMIAELG
                     ATTGVFPSDERTREWLRMQQREEDFVELSADAGADYDEREHIELNELEPLIAKPHSPG
                     NVVPVSEVAGTNAAQVCIGSSVNSGFEDLAVAAAVLRDESVSPGLVMTVTPGSRQILD
                     SIAATGVYTDLVRTGARMLEPVCGPCVGMGQAPPSDSVSVRTFNRNFPGRSGTMTDQV
                     YLTSPTTAVATALRGAITDPRELGEYPDLPEAPANPAVDDRQILAPAPPEEASGIEII
                     RGPNIEPPPEAPELPDSLACTVTIVVEDDISTGDLAPDGVEVMSFRSNVPEIAKFTFR
                     RFDPEYHGKAQEMAPGLILGGHNYGQGSSREHAALAPLHLGIRAVIVKSFARIHRRNL
                     ISQGILPLRFEDESNYDRFEPGQVWELQGIRDRLKKGDVEVPLKKGDDEVTLLAEYSQ
                     RERDILLAGGILRQLREKAEDEQPEAPQRVSGEAEL"
     gene            complement(7479..7970)
                     /gene="lspA"
                     /locus_tag="VFQ10_05785"
     CDS             complement(7479..7970)
                     /gene="lspA"
                     /locus_tag="VFQ10_05785"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET9964884.1"
                     /translation="MTRRTGLLFSLALALVVFVVDQSIKSVIEGSMRVGESITLVPGF
                     LSLTYIENDGGAFGILGGSQMVLLLGSTVAVVVVLWMLLSGRPSMLTMLGCGLILGGA
                     AGNLLDRLSSGEVTDYVHFSFWYIFNAADAAIVVGVGLLLLSALRPERTGQLTGSERK
                     ADP"
     gene            complement(8041..>8687)
                     /locus_tag="VFQ10_05790"
     CDS             complement(8041..>8687)
                     /locus_tag="VFQ10_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9964885.1"
                     /translation="HHETSSMKDGHPIFAALYDQKSASAERRWMGERRRRLLTGARGA
                     VLEIGGGTGANLAHYRNVDRVTIAEPDPFMRNRIGPKLEEARVPVEVSAAGAEALPYP
                     DGSFDTVVSTLVLCTVPDQEAALDEVRRVLRPGGRLLFIEHVRAAGSAARGQDRLEPL
                     WRRLLGGCHPNRDTVAAIEEAGFEIETFESFYPPDPLSALMPHVQGSATVRLAA"
BASE COUNT         1649 a         2631 c         2827 g         1580 t
ORIGIN      
        1 atgaaagcac tcgaggagcg gggcttctac cggcgcgagc ggcgcagttt cctggggatc
       61 atcccctacc agcgctgggc gctctccaga tcgggggagg ccgcgaggga gcagctcaag
      121 ttgaccatgc gtctcggcga gtcgcagttc ggcagatggg tggacaggga gccgaaccgg
      181 gctctggcgt acctcgggct cgttgggccg gcggtcctgc tcatggaacc cctgcacccg
      241 gatctccaac gtctgcgcga agtgcaagat cgaaccggcg atggtgtcgg aagcagtgga
      301 acaagcggag actcccaagg cgaggaatca tccggggaag acgcgcctga ctccggcgac
      361 ttcgacgtcg atttcgacgt gctgggtgat ctcggcggcg ctttcgacgc catagactcc
      421 ggcatggacg ccggaggcgg cgatggtgga gacggaggag gcaacggtgg tgactgacgt
      481 ggaatgcgct catggccgca ggcgtttgat ggaaagtgga taggatccct tgaagatcta
      541 tcgctcgata ctcgccgttc cagcgaacaa cccgcggatg gtcgagaacg cgctcgcctc
      601 ggccgccgac gccgtgttcc tcgacctcga agacgctgtt gcgcccgaca gcaaggccga
      661 agcccgcaaa gacatcgtct gcgctctcaa agagctcgag tggcggggac gccccaccgt
      721 gttccgggcc aacgccctgg acacgcctta cttctacagg gacctgatgg aggtggtaga
      781 aggagccggt gactctttgg acgccatcat gatcccgaag gtgaaccgcc ctgaggacct
      841 ctgcgctgtg tccatcctgc tctcccaact cgagctggcg atggatctgg aaaagggcag
      901 gatagagatc gaagcccaga tagagagtgc cgaagggctg gccaacgtag actccatagc
      961 ccgcgccacg ggtcgcctca cgaccctcca tttcggcccc ggagatttcg ctgctagcgt
     1021 gagcatgccc caaatcagca tcggtgtgac ggacgagtgg gacgaagcgt atcccggcca
     1081 ccgcttccac tacgccatgc agcgcatcgt cgtcgcggcc cgcgctgccg ggctgcgcgt
     1141 cctcgacggc cccgtcgctg actacggtga cgaagaaggg ctacgcagga gctgcctgat
     1201 tgcccgctct ctaggcttcg acggcaagtg gtgcatccat ccggcccaga tagcggtcgt
     1261 caacgagacc ttctcaccca ccgataagga agtggattgg gcgagaaaag tcgtcgcagc
     1321 ctacgatgag gcgaacgccg aaggctcagg ctcggtaagc gttgacgggc agatggtgga
     1381 cgcagcctct ataaagctgg ctcgcaacac gcttggtaag gtcccgaaga aggacacttg
     1441 agccgagacg gatgaggtca ccgctccact ggctcttcgc gccgtagggc ttctggcctg
     1501 acggcggtat aacatgaact gatgaacggg cccgagcaac tgccttacgt ggcgcaaccc
     1561 gcaggactga gcgcccgtac ggtgcgctgg ctgcgggtct tgttgctcgc tgcggtggca
     1621 gcggtgttca gtctgttgta tctcctctcg gcggagttcc ggtccgagat gaatcgggcg
     1681 ttggggatac tgggacgtgg agacatcgcc gggctcagag actacatcgt ctcgttcggt
     1741 ctctgggctc ctgtggcctc gtgttttctt atggtgttgc aggcgttggt tgctcccgta
     1801 ccgtccttcc tcatcacttt cgcaaatggg ctcgctttcg gggtgttctg gggctggatg
     1861 ctctccctgt tcgggcacgt gctcgcggcg gccgtgtgct tcgggatctc acgtgcttta
     1921 ggcagggtcc ctgttgaagt gctcgtcggg aagacggggc tcgaatcggc cgaccgctgg
     1981 ttcgcgcggt ggggcgtcta cgccgtgttc gccggacgac ttctacctgg tgttgccttc
     2041 gacgccatct cctacgccgc tggtctcacc cggatgcggt tcaggagctt catagtggcc
     2101 acggccctcg gcatcttccc ccagacgttc ctgtactcgt acctgggacg ccaggcgccc
     2161 gagtacgtcg ccctgttcct ggtgacctca ggtctcgtgg tcctcggagt agtaatggtc
     2221 gccgtcatca ggtacagggg ggaacgccgc aagcccaaaa ggaacctgcg ctagcgaagc
     2281 aggccctgtg gacggctacg tggcgggtag aataagcacc atgacacaaa tcttcgatcc
     2341 accgttcgac gcgtcgaccc atagaactgg atctctcatc gtcatcatgg gctcgatgtt
     2401 ctcaggcaag acagaggagt tgatccggcg cgtgcgccgc actctctacg ctcaccgcag
     2461 cgcacaggtc ttcaagcaca ccatcgacac ccgctcggag aactccgaga tacggtccca
     2521 caacggcgta ctgcacacag cactcgccgt ctcgacgagc gaagagcttc taaggaaggt
     2581 agaggagacc acggacgtcg tggccatcga ggaggcgcag ttcttcgacg aggggatagt
     2641 cgaagtctgc cggcgactgg ccgacgcggg atacgaggta atcgcggcgg gcctcgatat
     2701 ggatttcaag ggcgagccgt tcgggccgat gccgaggcta ctggccgagg ccgacgaggt
     2761 aacaaagctc agggccatct gcgcgcgctg cggcagagaa gcctcgcgct cgcagcgtct
     2821 catagatgga agaccggccc tcgtctccgc gccgaccatc ctgatagggg ccgaggagag
     2881 ctacgaagcc cgctgccgcc actgccacga ggtgcccgaa ggcgtgttcc agtacagatt
     2941 ccccggaata tagcgaaccg tggtgagggg ttaatgccgc gaaaatacat cgtagtgctg
     3001 atcctgttct gcatcacgtt gaacgcggct ggctttgtgt ggaaactctt cgagcccgtt
     3061 ctgttctacg acgaggtcgc ccacttcatc acgccgttca cactcgtgct gatactggcg
     3121 gagatcatct accgcactgg gggagacgac gaattcttca acactcccat gcgggccatc
     3181 atcacaggct ccgtcatcgg gctcctgggc gccacaggtt gggaaggtat cgaggtcctg
     3241 atgtcctgga tgggcataag cgtatccaac gccctactcg atacgatctt cgatatcttc
     3301 ctcggcgtcc tcggcggcgc tgctggcgcg tgggccgctg atcgctatct cgaccgtatc
     3361 ttcggacgct ctcgcacgaa cgcccaccag ccccgagtcc gttagagcaa ttcagcacgc
     3421 tacgcccttc gggcttcgct cgtcagccag gactcttgcg cacgcgaaag atgacgtgct
     3481 gactaactga aatgcgccat ttgcagaagt tgaccttctg caaatcgctc caggaagaca
     3541 cggcgacgtt acgagggtta catttcgggg gataatgcta gacagagaaa ccagcgggag
     3601 gaggagacgt gggcactacg gccttgatcg tgatcatagt ggtagttgtc gtcgtagtct
     3661 tgttacttgt gctgtacttt gcttcgcggc ggagggctgc tcagaggagc gaggagcagc
     3721 gggagcggag cagggcggag ttcggctccg agtacgagcg gacggcgcag gagataggct
     3781 ccgaggagga ggccgagagg gaattgaggg agcgtcgcgg tcgcgtagag cggcgggtcg
     3841 agccgctctc cgaagagagt cgcggtcgct atgaggagca gtggggagag gtcgagaggg
     3901 tcttcgtaga caatccggag aggtccatag agatggcgga ccggaccgtc tccgacatcc
     3961 tgaacgagcg caacttcgtc tccgacgcgg gccaatccga cgaggagacc gagcggggcc
     4021 tggccgccat gcaccctgag gtcgccgacg actacaggga ggcacggcgc atccgggccg
     4081 acgtcgtggc ccgttcagca gggcgcgcgg aggaggactc gaacgaggag tccaccgaag
     4141 agttgcggca agccatccgc aagtatcgcg cggtctacca gcggctcgtg cgagaatagc
     4201 gaggctgccg aaggggcgac gtgggagcgc tggctgcggg gttctgggga ttcgtcgggg
     4261 gagcagcgct ccttgtagga gcgctcatag gtatctacgc tggtgcttcg aggcgcacta
     4321 tcgcgacagt catggcgctc ggggctgggg tcttgatctc ctcagtcgcc ttcgagctca
     4381 tggacgaggc ctaccagaga ggaggcttcg acgcgtcagc cctggggctc ttgctggggg
     4441 ccgtggcctt cttcttcgcc gacgtggctg tgaacaggag gggaggggca caccgcaaga
     4501 accctggaga taaacagggt gagtcggcga gcgccatcgc aataggcgcg ctcatggacg
     4561 gcatcccgga gtcggcggct atagggatca gcctcctcga agggggcaat atcagcgccg
     4621 ccctcgtcgc ggccgttttc ctctcgaatg tcccggaagg actatcttca gcctcgggga
     4681 tgaaacaggc cgggcgccct gcagcctata tcttcgcgct ctggagcgga gtaacggtag
     4741 cctccgcatt ggccgcgttg ctaggctact tcttcctcgc cagcgcctcg gaggatgtag
     4801 tcgccacgat tcaggccttc gccgccggag ccatcctgac gatgctcgca agcaccatga
     4861 tgccggaggc atacgaggag ggcggttcgg tcgtcgggct cgccaccacg gcaggcttcc
     4921 tcctctcctt tgtcctgagc cacctcgagt agggtaggat cgtgaactca tagggatggc
     4981 ccgtccttga ggtgcgatcc cgctacacac ttcggaccat ttttgccgct atgggcgcca
     5041 aaggcgggga caggttttgt tgtgtaagtt actatcgagg cacatgtttg cgaaggagga
     5101 ggaccgatgg ctagtttggt gtgtccgtgc gcgcgagggg cagatctctg gggggagttc
     5161 tggtgggtag gtcgggagta caagtgggtg tttttcgacg acgacaacac tagcgagact
     5221 ttcaccgagc aagttacgca ctgttcaggg tgcggcacac ccctggatcg gaagaccctc
     5281 aaagtaacgt cccctatcgt gcgccaccaa tagcggaggg catcgctgac gtccttttcc
     5341 ggagttcggc accacgccgg atctactggg acgctgtccc aggatcgagc cagcgcagcc
     5401 ccgctacgaa tacatcgact catccgcggg caaaccacgg aagacgagaa tgtagaatga
     5461 gtagacgagt ttgggaacgc aagtcggggg tgatggacta tggcgaagaa tctggtcacc
     5521 aagatcctcg aggaccacat ggtcgaaggg tcgctagatc ccggcgagga agtagggctg
     5581 aggatagatc agacactgct tcaggacgcc acgggcacga tggcctgcct gcagttcgag
     5641 aggatgggcg tggaccgcgt ccaggtggat ctcgcggtcc agtacgttga ccacaacgtc
     5701 atacaactcg acttcaagaa cccggacgac caccgcttcc tgcagggctt cgccgcaaaa
     5761 tatggcatcc actactcccg ccccgggaac ggcatctgcc actacctgca cgtcgagcgc
     5821 ttcgccaggc ctggcgcgac gctcgttggg gcggattccc atactacttc ctccggcgct
     5881 ctagggtcca tagcgatcgg agccggcggt ctcgacgtgg cgctggtgat ggccggtcag
     5941 cccttccagt cccccgctcc caaggtcgtc ggcgtcgagc ttacaggggc cctctcagac
     6001 tgggtgaccc cgaaggatgt gatcctcgag ctcttgaagc ggcgcggcgt caggggaggc
     6061 ctcgggcgca tcttcgagtt ttacggttct ggagtcgcga acatagacgt aactggaagg
     6121 accacgatct gcaacatgat cgccgagctc ggcgccacca caggcgtctt cccctcagac
     6181 gagaggacgc gggagtggct gaggatgcag cagcgcgaag aggacttcgt ggagctctcc
     6241 gccgacgcgg gcgccgacta cgacgagcgc gagcacatcg agttgaacga gctggaaccc
     6301 ctgatcgcaa agccccactc gccaggcaac gtcgtcccag tgagcgaggt ggcaggcacc
     6361 aatgccgcgc aggtctgcat cggcagctcg gtcaactcag gcttcgagga cctggccgtc
     6421 gctgctgcgg tcttgcggga cgagtcggtc tctcccggtc tcgtcatgac cgtgacgccg
     6481 gggagtcgtc agatcctcga ctccatcgcc gctaccggcg tctacaccga cctggtgcgg
     6541 actggtgcgc ggatgctcga gcctgtgtgc ggtccttgcg tcgggatggg acaggcgccg
     6601 ccttccgatt cggtcagcgt caggaccttc aaccgtaact tccctggacg ctcggggacc
     6661 atgaccgacc aggtgtacct gacgagtccc accacggcgg tcgcgaccgc tttgcggggc
     6721 gccatcacgg accctcgtga gctcggggag tacccggatc tccccgaggc tcctgcgaac
     6781 cctgcggtgg acgaccgcca gatcctggcc cccgcgccgc cggaggaggc atcggggatc
     6841 gagatcatac gtggcccgaa catagagccg ccgccagaag cccctgagct ccccgactcc
     6901 ctggcgtgca cggtcaccat cgtagtcgag gacgacatct ctacgggcga cctcgcgcct
     6961 gacggggtcg aggtaatgtc gttccgctcg aacgtgcccg agatagccaa gtttaccttc
     7021 cgccgcttcg acccggagta tcatgggaag gcccaggaga tggcgcctgg ccttatcttg
     7081 ggcgggcaca actacgggca gggatcgagt cgcgagcacg ccgctctggc gcctttgcac
     7141 ctcggtatca gggcagtcat cgtgaagagc ttcgcccgca tccaccgccg gaacctcatc
     7201 tcccagggca tcctcccctt gcgtttcgaa gacgagtcga actacgacag gttcgagccg
     7261 ggccaggtgt gggagctgca ggggattagg gaccgcctga agaaaggcga cgtagaagtc
     7321 ccgctgaaga agggagacga cgaagtaacg ctcctcgccg agtactcgca acgagagcgc
     7381 gacatccttc tcgccggcgg catactgcgc cagttacgcg agaaggcgga ggacgaacag
     7441 cccgaggctc cccagcgagt ctccggcgaa gccgaactct aaggatcagc tttccgttcg
     7501 ctgcctgtca gctggccggt cctttcgggg cggagagcgg agagaagcag tagcccgacg
     7561 ccgacgacta ttgcggcgtc ggcagcgttg aagatgtacc agaagctgaa gtggacgtag
     7621 tctgtaacct ctccgctgct caggcggtcg agaaggttgc ctgccgcgcc gcccaggatc
     7681 aaaccgcacc ccagcatggt gagcatcgac ggtctccccg acagtaacat ccagagcacg
     7741 accacgacgg cgacggtgct cccgaggagg agaaccatct ggcttccacc gaggataccg
     7801 aaagccccgc cgtcgttctc tatgtaggtg aggctgagga agcctggcac gagggtgatg
     7861 ctctccccga cccgcatcga gccctcgatg acggacttta tgctctggtc gactacgaag
     7921 accacgaggg cgagcgcgag ggagaagagc aggccagtcc ggcgggtcat gacgaagcgg
     7981 gatgcaattc aggaccactc atcggcgcat tgatcctagc ataccaccct cgctggattc
     8041 tcaggcggcg agccgaaccg tcgccgatcc ctgtacgtgt ggcatgaggg cagacagggg
     8101 atccggaggg tagaagctct cgaacgtctc tatctcgaac ccagcttcct cgatggcagc
     8161 cacggtgtct cgattggggt ggcagccacc gaggagccgc ctccacagag gctccaggcg
     8221 gtcctgcccg cgggccgctg agccagccgc gcgtacgtgc tcgataaaca gcagccgtcc
     8281 gcccggacgc aggacgcgcc tgacctcgtc cagtgcagct tcctgatccg ggaccgtgca
     8341 caggacgagc gtcgagacca ctgtatcgaa gctcccgtca gggtagggta gcgcttccgc
     8401 cccggccgcc gagacctcga cagggacgcg ggcttcctcg agcttcgggc cgatcctatt
     8461 acgcatgaaa ggatcgggct cggcgatggt tacccgatct acattacggt agtgcgccag
     8521 gttagcgcct gtgccgccac cgatctcgag cacagcgcca cgggcaccgg tgagcaatcg
     8581 cctcctgcgc tcccccatcc accttctctc cgcggaggcg cttttctggt cgtagagcgc
     8641 agcgaagatg ggatgtccgt ctttcattga actggtttcg tgatggt
//
LOCUS       DASTKM010000134         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_561609, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000134 DASTKM010000000
VERSION     DASTKM010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561609"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFQ10_05795"
     CDS             complement(<1..271)
                     /locus_tag="VFQ10_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9964886.1"
                     /translation="MRIEENTFLVAGSGSGLGEATAHMLAASGARVVVADLKGEAPEG
                     GHFVETDVTDEGSVGAAVNAALEVGNLRGAVNCAGVASAEKVLGRE"
     gene            complement(275..1123)
                     /locus_tag="VFQ10_05800"
     CDS             complement(275..1123)
                     /locus_tag="VFQ10_05800"
                     /inference="COORDINATES: protein motif:HMM:NF004826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET9964887.1"
                     /translation="MNVSKAVLITGCSSGIGWATAERLVDVGWRVYATARSVEKMAPL
                     EQRGCGLLPLDVTDEDSMRSAVDEVERRDGPVGVLVNNAGYSQSGAVESVPMEKVRRQ
                     FETNFFGLARMCQLVLPGMRRQGYGRIVNVSSMGGKLTFPGAGYYHATKHAVEALSDA
                     LRFEVQGFGVKVSVIEPGLIRTDFAETAVGSMDDSDGENPYAGFDESVAKATAENYER
                     GPFARLGGGPEAVAEAIEHAVSVRSPRSRYAVTPSARLFLRLRRLLPDGAWDAALRTI
                     YPQPGR"
     gene            complement(1128..2873)
                     /locus_tag="VFQ10_05805"
     CDS             complement(1128..2873)
                     /locus_tag="VFQ10_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9964888.1"
                     /translation="MVEILLQKNVPAEMRDGTTLMSNVYRPAGDDNYPVLLTRLPYGK
                     DLLSEGSVLDPVMAAETGYIVVVQDVRGRYRSEGKFVPFVKEYEDGYDTVEWAAKLPD
                     SNGSVGMYGLSYFGKTQWHAAIMRPPSLKSMVPGLTWGNHLNGVQMRGGVQELGLMQY
                     WAQTALTLDLLFRKYAGDREIVGEKLPEVIHTIDTLLAGGGYDVLPLADLPNPDELVP
                     FVEGGFGRSVHHEDWDYLNIEGKYENVDAPTFHIGGWYDCFIGETLRQYEAMKERSRE
                     AGLRPPHLLVGPWTHGDFGSTFGDLDFGIGSSGMFLDYRGDLTDAHLRWFDATLKGEE
                     SALERTPPVRVFVMGENRWRGYEEWPSPGSHGEDWHLISDGSLRREPGGAGDEPARYD
                     YDPEDPVPTVGGPVLMAAIHGSGPRDQRPIESRPDVLVYTSPTLTSPLTVLGSVYVTL
                     FAASSAPDTDFVARFVDVYPDGRAICVADGILRASARESYPAPGMVRPVAPSHIEPGE
                     IYEYIIDLWATGITFLPGHRMRVEITSSSHPRWERNLNTGESTLESSRTEVAHQTIFH
                     DAGRPSRITLTVVGG"
BASE COUNT          486 a          958 c          918 g          546 t
ORIGIN      
        1 cctctcttcc caggaccttc tccgcgctgg cgaccccggc gcagtttacg gcgccgcgta
       61 gatttcccac ctccagggcc gcgttcacgg ctgcccctac gctgccctcg tcggtgacgt
      121 cggtctccac aaagtggcca ccttcaggag cctcgccttt tagatcggcg accacgacgc
      181 gcgctcccga cgcggcgagc atgtgggctg tagcttcccc caaaccggag ccactgccgg
      241 ccacgagaaa cgtgttctcc tctatgcgca ccttctacct ccccggttga ggatagatag
      301 ttctcaaggc cgcatcccag gccccgtctg gcaggagacg cctgagccgc agaaacaggc
      361 gcgccgaagg tgtgacggcg tagcggctcc tggggcttcg gaccgagacc gcatgttcta
      421 tagcttcggc gaccgcctcg ggtccgccgc ccagcctcgc gaagggaccc cgctcgtagt
      481 tctccgccgt cgctttggcg acgctctcat cgaagccggc gtacgggttc tccccgtctg
      541 agtcgtccat cgagccaacg gcggtctctg cgaagtcggt cctgatgagt ccgggctcta
      601 taacggagac cttgacgccg aagccttgga cttcgaagcg cagggcgtcg ctcagcgctt
      661 cgacggcgtg cttggtcgcg tggtagtagc cggcgccggg gaacgtcagc ttcccgccca
      721 tggaggagac gttgacgatc ctgccatatc cctgccgccg catccctggc aacacgagct
      781 ggcacatccg cgcgagcccg aagaagttcg tttcgaattg tcgtcggact ttctccatag
      841 gcaccgactc cacggcccca gactgactgt aaccggcgtt gttgacgagt acgccgacgg
      901 gcccatcccg ccgttcgacc tcatcgacgg ccgagcgcat agagtcctcg tcggtcacgt
      961 cgaggggcag gagtccgcaa ccacgttgtt cgaggggagc catcttctcg acactgcggg
     1021 ctgtggcgta gactctccag cccacatcca cgaggcgctc ggcggtggcc cacccgatgc
     1081 ctgaggaaca gccggtgatc agaaccgctt tcgagacgtt catctctcta ccctcctact
     1141 acggtcagcg tgatcctgct cggtcgcccc gcatcgtgga agatggtctg gtgggctacc
     1201 tcggtgcggg aggactctag tgtgctctcg cccgtgttga ggttgcgctc ccagcgggga
     1261 tgggaactcg atgtgatctc cacccgcatc ctgtgtcctg ggaggaatgt gatgcccgtc
     1321 gcccagaggt ctatgatgta ctcgtagatc tcacccggct ctatatggga gggtgccaca
     1381 ggcctaacca tgccgggcgc agggtagctc tccctggcgc tggcccgcag gatgccgtcg
     1441 gcgacgcaga tcgcccgccc gtcagggtag acgtcgacga accgcgccac gaagtccgtg
     1501 tctggggctg atgatgccgc gaagagcgtg acgtagaccg aaccgaggac cgtaagagga
     1561 ctcgtcaggg tcggactcgt gtagacgaga acgtctggcc tgctctctat gggccgctga
     1621 tccctcggcc ctgagccgtg aatggcggcc atgaggacgg gaccacctac cgtcgggacg
     1681 gggtcctcgg ggtcgtagtc gtagcgcgca ggctcgtctc cggcaccgcc cggttcgcgg
     1741 cgcagcgaac cgtccgagat caggtgccaa tcctcaccat gtgaccctgg agacggccac
     1801 tcctcgtagc cgcgccagcg gttctctccc atgacgaaca cccttaccgg aggcgtacgt
     1861 tcgagtgcgc tctcctctcc tttcagggtg gcgtcgaacc agcgaaggtg ggcgtctgtc
     1921 aggtcgcccc tgtagtccag gaacatccct gagctcccga tgccgaagtc gagatccccg
     1981 aatgtgctgc cgaagtcccc gtgcgtccac gggccgacca aaaggtgcgg cggacgtagc
     2041 cccgcttccc gagagcgttc tttcatcgcc tcgtactggc ggagcgtctc cccgataaag
     2101 cagtcgtacc agccgccgat atggaaggtt ggcgcgtcca cgttctcgta cttgccctcg
     2161 atgttgaggt aatcccagtc ttcgtggtgt acgctgcgcc cgaaacctcc ctccacgaag
     2221 gggactagct cgtccgggtt cgggaggtcc gcgagcggca gtacgtcgta gccgccacca
     2281 gccaggaggg tgtctatggt gtggattacc tcagggagct tctccccgac gatctcgcgg
     2341 tcgccggcgt acttgcggaa gaggaggtcg agggtgagtg ctgtttgcgc ccagtattgc
     2401 atcaggccga gctcctggac gccgccgcgc atctggacgc cgttaaggtg gttgccccag
     2461 gtgaggcctg ggaccatgct cttcagcgag ggcggccgca tgatggcggc gtgccactgg
     2521 gtcttgccaa agtacgagag gccgtacatg ccgaccgacc cgttggaatc ggggagcttc
     2581 gcggcccact ccacggtgtc gtagccgtcc tcgtactcct tgacgaaggg gacgaacttt
     2641 ccctccgaac ggtaacgtcc gcgcacgtcc tgcacgacga cgatgtagcc ggtctccgcg
     2701 gccattacag gatcaaggac cgacccctcc gaaaggaggt ccttgccgta ggggaggcgc
     2761 gtcaggagga cggggtaatt atcgtcgccg gcaggccggt agacgttgga catcagggtc
     2821 gtgccatcgc gcatttcggc cggcacattc ttctggagga ggatctctac catccggcgc
     2881 gcctacggag cagcacgaga gcagccgc
//
LOCUS       DASTKM010000135         7511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1443507, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000135 DASTKM010000000
VERSION     DASTKM010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443507"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFQ10_05810"
     CDS             complement(<1..687)
                     /locus_tag="VFQ10_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9964889.1"
                     /translation="MIDPTPALFGLLVGFLVGLTGVGGGSLMTPFLVAVMGVPAPTAV
                     GTDLTFATLTKLTGSFQHFRQRSVNLEIAVFLGMGSIPAGLLGVGTLEWIKGSFDPAR
                     VESIMITIIAATLVLVGVSLIYRDYFMPKRREGPKPGKWNGKGRMSRRRRAYTVLFGA
                     LGGYLVGLTSIGSGSVMAVILLLLYPIAPAVIVGTDITHAMVLSFVVGIAHMTQGNVD
                     FALAGTLLLGS"
     gene            complement(694..1266)
                     /gene="cysC"
                     /locus_tag="VFQ10_05815"
     CDS             complement(694..1266)
                     /gene="cysC"
                     /locus_tag="VFQ10_05815"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET9964890.1"
                     /translation="MENGTRVGRGFTLWFTGLSGAGKTTISEIVEHELRNRFRKIEVL
                     DGDIVRTNLSKGLGFSREDRDTNVLRIGFVADLLTRNGVGVIVSAISPFKEVRDQVRR
                     NIGEGFIEIFVDAPLEVCAERDVKGLYEKAFAGEIPQFTGVSDPYEAPAAPELHIKTD
                     EEEPKESAARVIVHLEELGYLTPVGALNRA"
     gene            complement(1319..2524)
                     /gene="sat"
                     /locus_tag="VFQ10_05820"
     CDS             complement(1319..2524)
                     /gene="sat"
                     /locus_tag="VFQ10_05820"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HET9964891.1"
                     /translation="MNVSTEFTTIAPHGGELVDRRAPEDQRDELLSRAEGFAKVTLGP
                     RALSDLEMISTGVFSPLTGFMVGEDYGSVVETMHLKDGLVWSLPITLPVSEEEADGIR
                     VGEEISLSDEAGRVLATMIVEDRYSYRKEHEAGEVYGTTSPEHPGVAAVYRQKDVLLG
                     GDVTLLVDDPNPKPFPTYHYEPRRLREVFADKGWKRVVGFQTRNPVHRSHEYIQKSAL
                     EIVDGLLLNPLVGETRSADIPADVRMRSYETILKRYYPKDRTMLAVFPAAMRYGGPRE
                     AIFHAICRKNYGCTHFIVGRDHAGPGKDSSGRPFYGDYDAQHIFDEFEEGEIGLTPLF
                     FEHAFFCLQCQGMGTTKTCPHAKENHVFISGTKVREMLSKGEYPPPEFSRPEVIEVLI
                     KGQRTQQDC"
     gene            complement(2521..2862)
                     /locus_tag="VFQ10_05825"
     CDS             complement(2521..2862)
                     /locus_tag="VFQ10_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964892.1"
                     /translation="MEGDVKRLSVPDEPIEIKARTRTLLEDSPEEGSRVIADAAFVSD
                     LLWGEWRADLEGAGMGYDRFLEISRSYAGEFRLWVVGERPWDHCAAGEAGRLLRRLPA
                     RQATDLAEVNG"
     gene            complement(2863..4053)
                     /locus_tag="VFQ10_05830"
     CDS             complement(2863..4053)
                     /locus_tag="VFQ10_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HET9964893.1"
                     /translation="MAGFDIREMMNDIDEAPGKVWFWDLEKAVIDADRCVRCGVCVAA
                     CPTDSIGIGEDDLPELVKMCTGCSLCWDFCPRGGLQYESTWKITGGSNGRGMGRVEES
                     YTARVRQRIDGVQDGGFVSALLVSLLEAGEIDGALLARESATERWKGEAFLATTPQEV
                     RACAGSFYNQTLALGHVDFEDYDLPPNPRIAVVGTPCEIEGIKAMQARPWTWGSSKVE
                     AITLTIALLCTKSFNYEKLMLEEIRDKRNVDLDNVGRVDVIRGKMVVQDLQGVTIFEE
                     PIRDFHGAALKGCDECADFMGHAADISVGSVGSADGYSSVLLRSEEGLAAFDSVRDRL
                     ELRPLDKPQALDKLDGLDKRVAFSSLKRAFDPDAPLFIDYEEHLNDYAGTDRAPVEHE
                     AVRY"
     gene            complement(4053..5708)
                     /locus_tag="VFQ10_05835"
     CDS             complement(4053..5708)
                     /locus_tag="VFQ10_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HET9964894.1"
                     /translation="MRYLRKDKTLNKVEKIKQERHPLEVYGAIIETYSKDLSAMDAVP
                     GEVERLKWVGIYPQKQGGDAFMMRIKIPGGVLTPEQTRVVGKIAADFARGPVPNPHFG
                     NNFLDLTTRQDVQMHWIKMENVPEIWRRLEEAGITTVQACGDSARNVLCCPVSGLGYE
                     EAIDAYPVAQAISAYFTGNREYANLPRKFKMSVTGCLEDCAQAEINDVGMLPARMEDG
                     TLGFNLRVGGGLSDGPRMASDIDVFVRPEEAVEITRGIAQVFGELGDRENRWTARMRY
                     LVQELGPEAFREELAKRVSVGLTPAGEDLTKRYRGDHIGVHPQRSRGGFQTRPYVYVG
                     LNVPVGRMSGEQFEEAGRLAGEYGDGVRLATDQNLIVTGVPEDRLDDLLSEPLLARYS
                     PKPGAFERGVVACTGSEFCHYGIVETKVRAVEWAREMDQRVGDPGQEAVRMHLSGCSA
                     SCAQPQIGDIGFRGETAKTPDAIVEGVDVGLGGSLGKDAAFIDWVEGAKPAGEVPDAL
                     VAVFEQFEKERREGERFHEWARRKKNAELREALRKPVESGVGS"
     gene            complement(5748..6506)
                     /locus_tag="VFQ10_05840"
     CDS             complement(5748..6506)
                     /locus_tag="VFQ10_05840"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HET9964895.1"
                     /translation="MHESLRSKHNDRILPGLGALERESELLEGADPREVIEWAVETYG
                     EGLALSASFGGGEGMALLDMISKVTDKVTVLTIDTGFIFKETAEFREEVMRRYKLPVE
                     VLKPELTIEEQVERYGEQMRTCTPNLCCQIRKIEPLQKALNRYDAWMTGIRREQTPQR
                     AGTKVVAWEERYQAAKIAPMPDWTEAQVWDYVRAHDVPVNPLLHQGYKSIGCEPQTRP
                     VHHDEDPRAGRWAGIDKTECGIHVEGGKISRAGA"
     gene            complement(6759..>7511)
                     /locus_tag="VFQ10_05845"
     CDS             complement(6759..>7511)
                     /locus_tag="VFQ10_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964896.1"
                     /translation="VLTITLGLALFLTMPERNFRPSPREGRSSLHHAATTARSGVRLV
                     RSRPVLLMLLAAALFAGMSEEGFDRLNPKQFLDVVGLPSVGGLDPVIWFGVIGAGGLV
                     LSYLAAGIVARNLDVSSPAVAARLLLALDALTMAGMLAFALAGSFAFALGTFWFASLV
                     RRVAEPVYLTWINEGLEPGVRATVISMSSQSGALGEASAGPVIGAIGNIFGVRPALTV
                     AAVILSPTLLLYVRAMKRPGVDPGAKDAKRVE"
BASE COUNT         1337 a         2479 c         2240 g         1455 t
ORIGIN      
        1 gctgcccaac agcagggtcc cggccagggc gaagtccacg ttgccctgcg tcatgtgggc
       61 tatgccaacg acgaacgaga ggaccattgc gtgtgtaatg tcagtgccga ctataacggc
      121 cggtgctatg ggatagagaa gaagcagaat gacggccatc acgctccccg agccgataga
      181 agtgagcccg accaggtagc cgccgagggc cccgaaaagg acggtgtaag cccggcgcct
      241 gcggctcatc cgtcccttgc cattccactt accaggcttc gggccctcgc gcctctttgg
      301 catgaagtag tcgcggtaga tcagggacac ccctaccaga acgagggtcg cggctatgat
      361 cgtgatcatg atcgactcga ccctcgcggg gtcgaaggag ccctttatcc actccagggt
      421 ccccacaccg agcagcccgg cagggatgct gcccatcccc agaaaaacgg cgatctctag
      481 gttgaccgag cgctgcctga agtgctggaa agatccggtc agcttggtga gcgtggcgaa
      541 ggtaaggtca gtcccaacgg cggtaggggc agggaccccc atcacagcta ccagaaatgg
      601 cgtcatgagc gacccacccc cgacccccgt gaggcctaca aggaaaccga cgagcagccc
      661 gaagagagca ggggtgggat ctatcacgct cgattaagcc cggttgagtg ctccgacagg
      721 cgtgagatat ccgagctctt cgaggtggac gatgaccctg gccgcgctct ccttgggctc
      781 ctcttcatcg gtcttgatgt gaagctcggg ggcggcgggc gcctcgtaag ggtcggagac
      841 accggtgaac tgcgggatct ccccggcgaa ggccttctcg tagaggccct tgacatcccg
      901 ctctgcgcaa acctccaacg gagcatcgac gaagatctcg atgaagccct ccccaatgtt
      961 ccgccgtacc tggtcacgca cctccttgaa gggcgagata gcgctgacga tcacacccac
     1021 cccgttgcgc gtcaacaagt cggcaacgaa gcctatcctc aagacgttag tgtccctgtc
     1081 ctccctggaa aaaccaaggc ccttggatag gttggtcctt actatgtccc cgtcaagaac
     1141 ctctatcttg cgaaagcggt tcctcagctc gtgctcgacg atctccgata tggtcgtctt
     1201 gccagcccca gataagcccg tgaaccacaa cgtgaagccg cggccaaccc tcgtcccatt
     1261 ctccattatt aacgcccctc cacttttgat tacgtactgt tgccatttta aggtattctt
     1321 aacaatcttg ctgggtccgc tgccccttta tgagcacttc gatgacttcg ggccgggaga
     1381 actcgggggg tgggtactcg cccttggaga gcatctcgcg gactttcgtg ccgctgataa
     1441 acacgtggtt ctctttagcg tgggggcagg tcttggtcgt ccccatcccc tggcattgca
     1501 ggcagaagaa ggcgtgttcg aagaaaagtg gggtaagtcc gatctcccct tcctcgaact
     1561 catcgaagat gtgttgggcg tcgtagtcgc cgtagaatgg tctgccgctc gagtccttgc
     1621 caggaccggc gtggtcgcgg cctacgatga agtgcgtgca gccgtagttc ttgcggcaga
     1681 tggcgtggaa gatggcttcc cgaggtccac cgtagcgcat cgccgcgggg aagacggcca
     1741 gcatcgtccg gtcctttgga tagtagcgct tcaggatcgt ctcgtaggag cgcatcctga
     1801 cgtcggcggg gatatccgcg gagcgggtct cgccaacgag ggggttcaag agcaatccgt
     1861 cgactatctc cagggcgctc ttctggatgt actcgtgcga gcggtgcacg gggttacggg
     1921 tctggaaacc gacgacgcgc ttccagcctt tgtccgcgaa gacctcgcgc agacggcgcg
     1981 gctcgtagtg gtaggtcggg aaaggcttcg ggttcgggtc gtccacgagc aacgtcacgt
     2041 cgccaccgag aagtacgtcc ttctgacggt agacggcggc gacgcctgga tgctccgggc
     2101 tcgtagtccc gtagacctcg ccggcctcgt gctccttgcg gtatgagtag cgatcctcga
     2161 cgatcatggt ggcgagcacc cgtcccgcct cgtcggagag ggagatctcc tcgccaaccc
     2221 tgatcccgtc agcctcttcc tcgctcacgg gtagtgtgat cggcaggctc cacaccaggc
     2281 cgtccttgag gtgcatcgtc tcgacgacgc tcccgtagtc ctcgccgacc ataaagcccg
     2341 tgaggggtga gaagacgccg gtggagatca tctccaggtc tgagagagcc ctaggaccca
     2401 gggttacctt ggcgaagccc tccgccctac tgagtagctc gtcacgctgg tcctcggggg
     2461 ccctgcggtc gacgagctcg ccgccgtgcg gggcgattgt agtgaattcg gtgctaacat
     2521 tcatccgttt acctccgcga gatcagtggc ctgcctggcc gggagtctac gcaacaagcg
     2581 gccggcctcg ccggccgcgc agtggtccca tggacgttcg ccgaccaccc agagtctgaa
     2641 ttcgcccgcg taagagcgcg agatctccag gaaccggtcg tagcccatgc cggctccttc
     2701 gaggtcggcc ctccattctc cccagaggag atcagagaca aaagcggcgt cggcgataac
     2761 gcgactaccc tcctcgggcg aatcctcgag tagggtacgc gtcctggcct ttatctcaat
     2821 gggctcatcc ggaacagaga gcctctttac atcgccttcc aactagtacc tcaccgcttc
     2881 gtgttccacc ggggcgcggt ccgtccctgc gtaatcgttc aggtgttcct cgtagtctat
     2941 aaacagcggg gcgtctgggt cgaaggcccg cttgagacta ctgaaggcca ccctcttgtc
     3001 caagccgtct agcttatcca aagcctgcgg cttgtcaagg ggccgaagct ccaatctatc
     3061 cctgacgctg tcgaaggcgg ccaggccttc ttcgcttcgg aggagcacgc tggagtaacc
     3121 gtccgcgctc cccacgctcc ccacggagat gtcggcggcg tgccccatga agtccgcgca
     3181 ctcgtcgcaa cccttgaggg cagcaccgtg gaagtccctt atcggctcct cgaagatcgt
     3241 cacgccttgg agatcctgta cgaccatctt ccctcgtatg acgtccaccc gtcccacgtt
     3301 atccaggtcg acgttcctct tgtccctgat ctcctcgagc atcaacttct cgtagttgaa
     3361 gctcttggta cagagcaggg ctatggtgag cgttatggcc tcgaccttcg agctgcccca
     3421 cgtccatggc ctggcctgca tcgccttgat gccctcgatc tcacacggcg tccccacgac
     3481 cgcgatcctg gggttcggcg gcaggtcgta gtcctcgaag tccacgtgcc cgagcgcaag
     3541 cgtctggttg tagaagctcc ccgcgcacgc gcgcacctcc tggggcgtcg tcgccaggaa
     3601 agcctcgccc ttccagcgtt cggtcgcgct ctcgcgtgcc agcagggcgc cgtcgatctc
     3661 gccggcctcg agcagcgaga ccaggagcgc actgacgaag cccccgtcct gcacgccgtc
     3721 tatcctctgc ctcacgcggg ccgtgtagct ctcctcgacg cgccccatgc ccctaccatt
     3781 agagccgcca gtgatcttcc aggtcgactc gtactgcagg ccgccgcggg ggcagaagtc
     3841 ccagcataga gagcagccgg tgcacatctt gacgagctct ggaagatcgt cctcgcctat
     3901 gccgatggag tcggtagggc aggccgcgac gcacacgccg caccgcacgc agcggtcggc
     3961 gtcgatcacc gccttctcca ggtcccagaa ccagaccttg cctggagcct cgtcgatgtc
     4021 gttcatcatc tccctgatgt cgaagccagc cactatgagc ccaccccgct ctcgaccggc
     4081 ttgcgaagcg cctcgcgcag ctcggcgttt ttcttgcgcc tggcccattc gtggaagcgc
     4141 tcgccctctc gccgctcctt ttcgaactgc tcgaagacgg ccactagggc gtcagggacc
     4201 tccccggcag gcttcgcgcc ctcgacccag tcgataaacg cagcatcttt gccaagagag
     4261 ccgccaagtc ctacgtccac gccctcgacg atcgcgtcgg gcgtcttcgc ggtctcgccg
     4321 cggaagccta tgtccccgat ctggggctgc gcgcaggagg ccgagcagcc cgataagtgc
     4381 atcctcaccg cctcctggcc ggggtcccca accctctgat ccatctcgcg ggcccactct
     4441 actgccctga ccttggtctc gacgatgccg tagtggcaga actcgctccc cgtacaggcc
     4501 acgacgccgc gctcgaacgc cccaggcttc ggggagtacc gcgccagaag cggctcggac
     4561 agaaggtcat cgaggcggtc ctcggggacg ccagtaacga tcaggttctg gtccgtagcg
     4621 agacggaccc catccccgta ctcccctgct agtctgcccg cctcctcgaa ctgctcacca
     4681 ctcatcctcc caacaggcac gttgagacca acataaacgt aggggcgggt ctgaaacccg
     4741 cccctactac gctgggggtg cactccgatg tgatccccgc ggtacctctt cgtcaggtcc
     4801 tctccggcgg gtgtgagccc tacggagacg cgcttcgcaa gctcttcgcg gaaggcctcg
     4861 ggaccgagct cctggaccag gtagcgcatt ctcgccgtcc agcggttctc cctgtcccca
     4921 agctcgccga agacctgggc tatgccgcgg gtgatctcca ccgcctcctc gggcctgacg
     4981 aagacgtcga tatccgaggc catcctcggg ccgtcggaga ggccgccgcc cacacgcaga
     5041 ttgaacccta gcgtgccgtc ttccatacgg gcagggagca tgcctacatc gttgatctca
     5101 gcctgtgcgc agtcctccag gcatcccgtt acgctcatct tgaacttcct cggcaggttc
     5161 gcgtactcgc ggttgccggt aaagtaggcg gagatagcct gcgccacggg gtaggcgtct
     5221 atggcctcct cgtagccgag gcccgagacg gggcaacaaa gcacgttgcg ggccgagtca
     5281 ccgcaggcct gcacggtcgt gatgcctgcc tcctccaggc ggcgccagat ctcgggcacg
     5341 ttctccatct tgatccagtg catctgcacg tcctggcgcg tggtcagatc gaggaagttg
     5401 tttccgaagt gcgggttggg cacaggaccc cgcgcgaagt cggccgctat cttacctacc
     5461 accctggtct gctcgggcgt caggacgccg ccagggattt tgatccgcat catgaaggcg
     5521 tcgccgccct gcttctgcgg gtagatgccc acccacttca accgctcgac ctcgccaggg
     5581 acggcgtcca tggctgagag gtctttcgag taggtctcta ttatcgcccc atagacttct
     5641 aggggatgtc gctcctgctt gatcttctcg accttgttca gggtcttgtc tttcctgagg
     5701 tatctcatgt gtagaacctg ctcttttgag gagtgaatct ctgaagttca ggcgcccgcg
     5761 cggctgatct tgccgccctc gacgtggatg ccacactccg tcttgtcgat tcctgcccaa
     5821 cggccggccc tgggatcctc gtcgtggtgc acgggcctcg tctgaggctc gcagccgatg
     5881 ctcttgtagc cctggtgcag gagcgggttg acaggcacgt cgtgcgccct cacgtagtcc
     5941 cacacctgcg cttctgtcca gtcaggcatg ggcgctatct tggcggcctg gtagcgctcc
     6001 tcccaggcga ctaccttcgt gcccgccctc tgcggagttt gttcccggcg aatgcccgtc
     6061 atccaggcgt cgtagcggtt cagggccttt tggagtggtt cgatcttgcg gatctggcag
     6121 cacaggttcg gggtgcaggt gcgcatctgc tcgccataac gctctacctg ctcctcgatc
     6181 gtgagctcgg gcttcaagac ctccacaggc aacttgtagc gccgcatgac ttcttcgcgg
     6241 aactcggcgg tctccttgaa gataaagccc gtgtcgatgg tgaggacggt gaccttgtcg
     6301 gtgaccttgg agatcatgtc cagaagcgcc atcccctcgc caccgccgaa cgaggccgag
     6361 agggccaggc cctcaccgta ggtctctaca gcccactcta ttacctcccg cgggtcagcg
     6421 ccctccagta actcggactc ccgctcgagc gcccccagac cgggtaatat acggtcgtta
     6481 tgcttcgaac gcagggattc gtgcatcgcg cgatcctcca gtgtaaatcg tgtaatagaa
     6541 agtcaagggt tgagatcaag cagcgcacac gctacatcgc cgcgcgcccg cgcgaggggc
     6601 ctgagacatt cgccccttcg catgaccctg cgtaccgtgt aactccatgc gccggatact
     6661 aagcgcctaa cagccatacg tcaacataat tccgatgaaa tttatcggaa ttgaacaaat
     6721 tctgcccttt tccgacgaga aggaatctcc ctggccggct actccactct cttggcgtcc
     6781 ttcgctcctg ggtctacccc tgggcgcttc atggcgcgta cgtagagcaa aagcgtcggt
     6841 gagaggataa cggctgcgac ggtcagggct ggtctgacgc cgaagatgtt gcctatagcc
     6901 ccgatcacgg gcccggccga ggcttcgccg agggcgcctg actggctact catcgagatc
     6961 acggtggccc ggacgccggg ctctagaccc tcgttgatcc aggtcagata gaccggctcc
     7021 gcgaccctgc ggaccaggga ggcgaaccag aacgttccta gtgcgaacgc gaagctgccg
     7081 gccagcgcga aagcgagcat ccctgccatg gtgagggcgt cgagcgcgag aagcagccgc
     7141 gctgcgacgg ccgggctgct cacgtcgagg ttgcgggcca cgataccggc cgccaggtag
     7201 ctcagcacga ggcctccggc cccgatcaca ccgaaccaga tcacgggatc gaggccgccg
     7261 acggacggta gcccaacgac gtccagaaac tgcttggggt tgagccggtc gaaaccttcc
     7321 tcggacatgc cggcgaacag ggcggcggcg agcaacatca agagaaccgg cctggagcgg
     7381 acgagccgca cgccgctgcg cgccgtggta gccgcgtggt gtagcgagga gcgcccctcg
     7441 cgcggggagg gtcggaagtt gcgctcgggc atcgtgagga agagcgcgag gcctagcgtg
     7501 atggtgagga c
//
LOCUS       DASTKM010000136         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1268321, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000136 DASTKM010000000
VERSION     DASTKM010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268321"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1116
                     /gene="trpD"
                     /locus_tag="VFQ10_05850"
     CDS             94..1116
                     /gene="trpD"
                     /locus_tag="VFQ10_05850"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET9964897.1"
                     /translation="MRETLRRAVDGEVITEGEAERALETIMEGNAPPAATAALLTALR
                     VKGERVPEIVGFARAMRRFAAKVEAPEGVVDTCGTGGDAKGTINVSTAAAFVARGAGV
                     VVAKHGNRAATSQAGSADVLEALGAEIELSPEKVSRCIAEAGLGFMFARTHHPAMRFV
                     APVRAELPFRTVFNLLGPLTNPAGARRQLVGVFGGTYVRPVAEALRDLGAEKALVVHG
                     TDGMDEITVTDKTLVADVSEGAIEEYEISPEDFGLPRHAPDGLLGGDAHLNARILRDI
                     LTGEERGASRDIVLLNAGAAIHVSGKAKTVEEGVRLARDSISDGAAARALKDFIRTTR
                     RLSGTV"
     gene            1113..1880
                     /locus_tag="VFQ10_05855"
     CDS             1113..1880
                     /locus_tag="VFQ10_05855"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET9964898.1"
                     /translation="MKRATVLDELATAAVRRAQDLRSRTDLNSLYQEALLFQKRDFAS
                     ALSTPGLSVISEIKRASPSAGFIRDADSAVWGARYELEGAGCLSVLTEPERFEGSLED
                     LDAARNNTTLPVLRKDFTVDEAQILETGVRADAVLLIAALFEAAALARYVSLAVEVGL
                     TPLVEIHDEEEANLALESGARVIGVNNRDLRDFTVDLGTFERLAPRLAGATLVAESGV
                     KNPEDARRLRDAGADAVLVGEAAMRDPSLVARLASLP"
     gene            complement(1906..>2745)
                     /locus_tag="VFQ10_05860"
     CDS             complement(1906..>2745)
                     /locus_tag="VFQ10_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9964899.1"
                     /translation="RAGTEQIAGGQLGSEVRVEGHDEVASLAEEFNRMARALEEAVER
                     EREMEKARRELVAAVSHDLRTPLAATRALIEAVADGVTSDRETEERYLSSASRELEHL
                     SRLVDDLFELARIDAGLLQLTLEEASLHDLISDTISSFQPQAEQQGVRLEGEIVGDVD
                     PVLANPPRLQRVLHNLVSNALRHTPADGTVTLRATREGEEARVEVSDTGEGIVAEDLP
                     RVFERSFRAERSRTRAEREDSPGAGLGLAIARGLIEAHGGTMDVESDFGHGSRFRFTL
                     KRA"
BASE COUNT          474 a          919 c          897 g          455 t
ORIGIN      
        1 atcgagggcg tccagttcca ccccgagagc atcctgaccc gaagcggtca cgacctcctg
       61 aagaacttcc tggggctctg agccgtaccc ttgttgcgcg agacgcttcg cagagccgtc
      121 gacggggagg tcattaccga gggcgaggct gaacgcgcgc tcgagaccat catggagggt
      181 aatgctcccc ctgcggccac ggccgcgctc cttaccgcgc tccgcgtaaa aggggagaga
      241 gtacccgaga tcgtcggctt cgcccgcgcg atgcgccgct tcgccgcgaa ggtcgaagcc
      301 cccgagggcg tcgtcgacac ctgcggcacg ggcggcgacg cgaaggggac cataaacgtc
      361 tccacggcgg ccgcgttcgt ggcgcgtggg gccggcgtcg tggtagccaa acatggcaac
      421 agggcggcga cgagccaggc cggtagcgcc gacgtgctgg aggcgctcgg cgcggagatc
      481 gagctaagtc cggaaaaagt ctcccgctgt atcgccgagg ccggcctcgg gttcatgttc
      541 gcccgcacgc accaccccgc gatgaggttc gtggcccctg tgcgggcgga gctgcctttc
      601 aggaccgtct tcaatctgct cgggccgctg accaaccccg ccggggccag gcgtcagctc
      661 gtcggggtct tcggcgggac ctacgtgcgt cccgtcgccg aggcgctgcg ggacctcggg
      721 gccgagaagg cgctcgtcgt acacgggacc gacgggatgg atgagatcac ggtgactgac
      781 aagaccctcg tcgccgacgt ctccgaggga gcgatagaag agtatgagat ctcaccagaa
      841 gacttcggcc tcccgcgcca cgccccggac ggtctgctcg gcggcgacgc ccacctcaac
      901 gcccgcatcc tccgcgacat cctcacaggc gaggagcgcg gggcatcccg cgacatcgtc
      961 ctcctcaacg cgggagccgc gatccacgtt tcaggtaaag cgaagacggt agaagaaggc
     1021 gtgcgtctgg caagggattc catcagtgac ggggccgcgg cgcgcgccct gaaagacttc
     1081 ataagaacca cccgccgcct ctcggggacc gtgtgaagcg cgcaaccgtc ctcgacgagc
     1141 tggccacagc ggccgtaagg agggcgcagg atctgcgcag cagaacagac ctgaacagcc
     1201 tttaccagga ggcgttgctc ttccagaaga gagacttcgc cagcgctctc tcgacgccag
     1261 gcctctccgt gatctctgag ataaaacgcg cctctccctc ggcaggcttt atccgcgatg
     1321 ccgattcggc ggtgtgggga gcccgctacg agttagaggg agcgggttgc ctctcggttc
     1381 tcaccgagcc tgaacgcttc gagggaagtc tcgaagacct cgacgccgcg cggaacaata
     1441 ctactctgcc tgtcttacgc aaggacttca ccgtcgacga ggcgcagatc ctcgaaacag
     1501 gggtcagggc cgacgctgtc ctgctcatag cggcgctctt cgaagcggcg gcactcgccc
     1561 gctacgtctc cctggccgta gaggtcggac tgacaccgct cgtggagatc cacgacgagg
     1621 aggaggcgaa cctggccctc gaatcaggcg ccagggtgat cggcgtcaac aaccgagatc
     1681 tcagagactt caccgtagac ctcggtacct tcgagcgcct cgcccccaga ctggccggcg
     1741 cgaccctcgt cgccgagagc ggtgtcaaga accccgaaga cgcccgaagg ctacgagacg
     1801 caggtgccga cgcggttctg gtaggcgaag cggcgatgcg agatcctagt ctcgtcgcac
     1861 gccttgcgtc cctgccgtga gcaacgttcg cgaaccacca gggtattagg ctcgcttcag
     1921 cgtgaagcgg aagcgcgagc cgtgcccgaa gtcgctctcg acgtccatag tgccgccgtg
     1981 ggcctcgatc aggccgcgcg ctatagccag gccaaggccg gccccggggg agtcttctct
     2041 ctccgcgcgg gtccttgacc tttcggcgcg gaacgagcgt tcgaagacac gcggcaggtc
     2101 ttcggcgact atgccctcgc ccgtgtcgga gacctcgacc cttgcctcct ccccttcgcg
     2161 cgtcgctcgc agggttacgg tgccatctgc cggggtatgg cgcagggcgt tggagacgag
     2221 gttgtggagt acgcgctgta gccttggtgg attagcgagg acggggtcaa cgtcgcccac
     2281 gatctcgcct tccagccgca cgccctgttg ttcggcctga ggctggaagc ttgaaatagt
     2341 gtcggagatc aggtcgtgca gcgaagcctc ttccagcgtg agttgtagca aaccagcgtc
     2401 tatcctggcg agctcgaaga ggtcgtcgac caggcggctc aggtgctcca gctcacgact
     2461 ggccgaggag aggtagcgct cctccgtctc cctgtccgag gtgacgccgt ctgcgacggc
     2521 ttcgatcagg gccctcgtcg cggcgagcgg agtgcgcagg tcatgtgaga cggcggcaac
     2581 gagttcgcgc cgcgctttct ccatctcacg ctcgcgctct accgcttcct ctaacgcccg
     2641 cgccatgcgg ttgaattcct ctgcgaggct ggcgacctcg tcgtggccct cgacccggac
     2701 ctccgaaccc aactggccgc ccgcgatttg ttccgtcccg gccct
//
LOCUS       DASTKM010000137         3690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_562816, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000137 DASTKM010000000
VERSION     DASTKM010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562816"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFQ10_05865"
     CDS             complement(<1..618)
                     /locus_tag="VFQ10_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET9964900.1"
                     /translation="MPTVVQPAVVEADRSSGLASSYLRLVRPRQWTKNGFVLAGVVFA
                     EKAFSVTAVANALLAFVIFCVLSGAVYAANDVLDVEEDRKHPLKRFRPVASGEISARA
                     ALAFAAVLAAGALVLSFTLLPPGVGIAGVAYLVLQVFYTTILKHAAILDVMSIAGGFV
                     IRALAGVAAVSSPISPWLIVCTGLLTLFLGFSKRRHEIATLGEGAV"
     gene            803..1702
                     /gene="menA"
                     /locus_tag="VFQ10_05870"
     CDS             803..1702
                     /gene="menA"
                     /locus_tag="VFQ10_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HET9964901.1"
                     /translation="MNPTKRQVFEEWFRLARPFSLTAAAVPVFFGTALAFKDGSFAWG
                     PFFAMLLGSLLIQAATNMFNEFYDAHRGLDVAGTVGIAGSIVSGRVSSRRVLAGALLC
                     YTLALFLGIYLIAVGGWPILVLGCLSALGGYLYSAGPRPIAYTPASEAAVFVFMGILI
                     VIIAYAVQARGFPPYVPLAALPIGGPVAAILLANNIRDLVSDRRGGRRTLPVVFGREV
                     GILVYRALLLEAYVAVAVLMAFGVVPPECALVFPSVFAAFRLWRDVASYSTPRRLDPV
                     VKRTAGLHLVFGLLYTAGVLIGA"
     gene            1695..2171
                     /locus_tag="VFQ10_05875"
     CDS             1695..2171
                     /locus_tag="VFQ10_05875"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9964902.1"
                     /translation="MPEEHRPFRASYIVSGVAAERIFASLLDVQRFPEWAAGLRRCRA
                     LDPAGIAETKDLRPGVRLEFTLSAAGLTHEVASIVTVVEPPRRLEWNYVEGALGGGGW
                     LVEQEAPGAVRITVSTDYLIRPAWLDNLAHKPFFRRLTEDLLKRSMRRFDAHLRYA"
     gene            complement(2168..2998)
                     /gene="tatC"
                     /locus_tag="VFQ10_05880"
     CDS             complement(2168..2998)
                     /gene="tatC"
                     /locus_tag="VFQ10_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET9964903.1"
                     /translation="MTLIEHLDELRSRIIRVGIAFFGVAIVAWFFRERIFDWLLAPAG
                     GALQGKLNVTGVTEQLFTDMKLALYVGFLLTIPVLLYQVWAFVAPAVGDMGRAFTYTI
                     ISMASALFIAGVAFGYFIVLPIGTQFLLGWDTERFDPIITAQSYLPFVTRFLLAFGIV
                     FELPAATYVAAKLELVDAPLLKRYRKHAIILNTVLAAALTPGQDPYSMVLMAVPMIVM
                     YEVSILIARYVNPSTEVTAHELAREPDEHETEGEADETGIGDFEDTDDHPENGHERDL
                     "
     gene            complement(3082..3363)
                     /locus_tag="VFQ10_05885"
     CDS             complement(3082..3363)
                     /locus_tag="VFQ10_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9964904.1"
                     /translation="MFGSVGMPELIIGLVVILLLFGAKRVPELARGLGSGVREFKKGT
                     QEGEVENKKKEEEEKKEEQLEASKSEHDEETKLEEDSSVEAEKAEQKRS"
     gene            complement(3429..>3690)
                     /locus_tag="VFQ10_05890"
     CDS             complement(3429..>3690)
                     /locus_tag="VFQ10_05890"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9964905.1"
                     /translation="EKGEKQKELLKKAGDAFAASAEAEPDNAEAYLLAGQAYDQAGQP
                     ADAIEYYNGYLDLEPKDENAEKVEDRISALLEGGKSSAKAEP"
BASE COUNT          633 a         1190 c         1133 g          734 t
ORIGIN      
        1 gaccgcgcct tcgcccagag tagcgatctc gtgcctacgc ttggagaagc cgaggaagag
       61 cgtcaggagc ccggtacaga ctatgagcca cggtgagata ggactactta ccgcggccac
      121 accggcgaga gctcttatga cgaacccgcc cgcgatgctc atgacgtcga ggatcgccgc
      181 gtgtttgagg atggtcgtat agaagacctg caaaacgagg tatgcaacac cggcgatccc
      241 gacgccggga ggcagcaagg tgaagctcag gactagtgcg cccgcagcga gcacggcggc
      301 gaaggccagg gccgctcggg ccgagatctc cccgctcgcg acgggtctga acctcttgag
      361 cgggtgctta cggtcctcct cgacgtcgag cacgtcgttg gccgcgtaca cggccccgga
      421 gaggacacag aagatcacga aagcgagcag cgcgttggct acagcggtca ccgagaaagc
      481 cttctccgca aagaccaccc cggccagcac gaacccgttc ttggtccact gcctgggccg
      541 gaccagccgc agataggagc tcgcgagtcc actggaacgg tcggcttcca cgactgccgg
      601 ttgtaccaca gtaggcatca gctttcaggc tacaggtgtc aggtccgtcg aaagggcaac
      661 tgggatccgc gtcgctcggc acgaacccgt ggacgcgcca cgggcgaacc tacctgacgg
      721 ctgaagcctg atgctggcag gccgaaatcc gtgaacgggc cgattgcaaa cgtgaacgac
      781 tctctccaca attggccgga ctatgaaccc gacgaaacgg caggttttcg aagagtggtt
      841 ccgccttgcc aggcctttca gcctcactgc ggcggctgtg ccggtgttct tcgggaccgc
      901 gctggcgttc aaggacggct cgttcgcctg gggaccgttc tttgccatgc tcttaggaag
      961 cctgctcata caggcggcca ccaacatgtt caatgagttc tacgatgcgc atcgcggcct
     1021 tgacgtagcc ggcacggtcg ggatcgccgg ctctatagta agtggccggg tgagttcgcg
     1081 ccgggtgctt gcgggtgcgc tcctttgcta caccctggcg ttgtttctcg gcatctacct
     1141 gatagccgtg ggcggctggc ccatactcgt actcgggtgc ctctcggcgc tcggcggcta
     1201 cctctactcg gccggaccgc gccccatcgc ctacaccccc gccagcgagg ccgcggtttt
     1261 cgtctttatg gggatcttga tcgtcatcat cgcctacgct gtgcaggctc gtggtttccc
     1321 tccgtacgtg ccgctggcgg cgctgcctat tggtgggccc gtggccgcga tcctgctcgc
     1381 caacaacatc agggatctcg tctccgaccg tagaggcggg cggcggacgc tgcccgtagt
     1441 cttcggcagg gaggtgggga tcctcgtata ccgggccctc ttgctggagg cttacgtcgc
     1501 ggtcgctgtg ctgatggcct tcggcgtcgt cccccctgag tgcgccctcg tcttcccgag
     1561 cgtgttcgcc gccttccgcc tctggcgcga cgtggccagc tactcgacgc cgaggcgcct
     1621 tgatcccgtg gtcaagcgga cggccggcct ccacctcgtc ttcggattgc tctacacggc
     1681 gggggtcctc atcggtgcct gaagaacacc ggcctttcag ggcgagctac atcgtatccg
     1741 gggtcgcggc ggagaggatc ttcgcctccc tcctcgatgt gcaacgcttc cccgagtggg
     1801 cggccggcct ccgccgctgc cgcgcgctcg atcctgccgg catagcggag acgaaagacc
     1861 tacgtcccgg cgtcaggctg gagtttaccc tgagcgccgc cggactcacg cacgaggtag
     1921 cgagcatcgt tactgtggtc gagccgccac gccggctgga atggaactac gtggaaggcg
     1981 cccttggagg cggcggctgg ctcgtcgagc aggaggctcc tggggccgtc aggataaccg
     2041 tctcgacaga ttacctgatc agaccagcct ggctcgacaa cttggcccac aagcccttct
     2101 tccgcaggct gacagaggac ctgctcaaac gctcgatgcg ccgtttcgac gcgcacctga
     2161 ggtacgccta gagatcccgc tcgtgcccgt tctcagggtg atcgtctgtg tcttcgaagt
     2221 caccgatacc agtttcatcg gcctcgcctt cggtctcgtg ttcatcgggt tccctcgcaa
     2281 gctcgtgggc cgtgacctcg gtcgacgggt tcacgtagcg ggcgataagt atggagacct
     2341 cgtacatcac tatcatcggg acggccatca ggaccatgct gtatgggtcc tgtcctggcg
     2401 taagggcggc ggcgaggacg gtgttgagga tgatggcgtg cttcctgtac ctcttcagga
     2461 gcggagcgtc aaccaactcc agcttcgcgg ctacgtatgt cgctgcaggt aactcgaaga
     2521 cgatcccgaa ggccaggaga aacctcgtca cgaaaggcag atagctctgg gcggtaatga
     2581 tgggatcgaa gcgctccgtg tcccagccga gcaggaactg ggtgccgata ggcagcacga
     2641 tgaagtaccc gaaggccacc ccagcgatga acagcgcgga ggccatgctg ataatagtgt
     2701 aagtgaaagc ccggcccatg tcgccgaccg cgggggccac gaacgcccac acctggtaga
     2761 gaagtaccgg gatcgtaagc aggaacccga cgtagagcgc cagcttcatg tcggtgaaca
     2821 gctgctctgt aaccccggta acgttcaact tgccttgtag ggcaccgccc gctggggcga
     2881 gcagccagtc gaagatcctc tcccggaaaa accaggcaac gatggcgacg ccgaagaacg
     2941 ctatgccgac cctgatgatc ctcgaccgca gctcgtcgag gtgttcgatg agggtcatgc
     3001 gcatctcgtc gcgcggtccg cgcggacgcg aagggagtct caggcgacct gccatctgtg
     3061 acttacgaac ccgtaaggag tctagctacg cttctgctcg gccttctcgg cctcgacgga
     3121 ggagtcttcc tccagtttgg tctcctcgtc gtgctcgctc ttgcttgctt cgagctgctc
     3181 ttccttcttc tcttcttcct ctttcttctt gttctcgacc tcaccctcct gggtgccctt
     3241 cttgaactcg cgcacgccgc tgccgaggcc ccgggcaagc tcgggaacgc gcttcgcccc
     3301 gaagagcagg agtatgacga cgagtcctat gatcaactct ggcattccga ccgatccaaa
     3361 cacctttcct cctaacgagc tatctgcgga tctcacgcaa cattatacgc cctccgtcct
     3421 ggcgtggatc agggctctgc tttagcgcta ctcttgccgc cctcgaggag ggccgagatc
     3481 ctgtcctcca ccttctcggc attctcgtcc ttcggttcga ggtcgaggta gccgttgtag
     3541 tattcgatgg cgtcggcggg ctggcctgcc tggtcgtagg cctggcccgc aagaaggtag
     3601 gcttcggcgt tgtcgggctc tgcctctgct gaggccgcga aggcatcccc cgccttctta
     3661 agcagctcct tctgcttctc ccccttctcg
//
LOCUS       DASTKM010000138         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_34441, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000138 DASTKM010000000
VERSION     DASTKM010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34441"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFQ10_05895"
     CDS             complement(<1..328)
                     /locus_tag="VFQ10_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET9964906.1"
                     /translation="MNVAMMTRTALMAAVTAVAAQIAIPLPFSPDPFTLQVLAVILSG
                     LLLGSRYRALAQAIYVLVGAVGVPVFAQFSGGLGVVLGPTGGYLLSYPLAAAIAGLAA
                     HTAANAT"
     gene            470..1375
                     /locus_tag="VFQ10_05900"
     CDS             470..1375
                     /locus_tag="VFQ10_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HET9964907.1"
                     /translation="MDRSIETELPQARYSYGGDEYVFVELAEAMSLKVNFRAMAITNR
                     LREEDIPGVTDICPSNASYMIRIDPDELHPDDLISRLKEIDEEVGDVHGFELKTRIVD
                     VPVMIEDPWTHETLMRFRDRHQDPNATDLEYSARINGFDSKEGFIEALLGSPWLVTMI
                     GFVPGLPWCYQLVPPEEQIEVPKYVRPRTYTPERAFGFGGGFAVVYSVEGAGGYQLFG
                     RSPVPVLDVQQRLKDFRDSIVFPKPGDVWNYRRIDREEFDRLEVEVEEGTFEYRKKDF
                     VFHPDRALENPHAYNDEILGVLYGD"
     gene            1365..2342
                     /locus_tag="VFQ10_05905"
     CDS             1365..2342
                     /locus_tag="VFQ10_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HET9964908.1"
                     /translation="MAIRVRQPGLLTTVQDTGRFGEYALGMPPSGAMDVFSFQVGNYL
                     VGNEDGAAGLEITYFGPELEFTEDTVIAITGAEMPPKINGEEAPTWETLRVEEGDVLS
                     FDDLKNGARSYLAVAGGIDVPIFMHSRSTYTLIGLGGHEGRALQEGDELKVGEAPRNA
                     EGQVGKRADDDHIPTYPREAELRVIIGLASYRITEESMDEFLSIQWTVTTDADRVGYR
                     YQGGELAFVEREQPAGAGADPANVVDFGYPVGSIQVPGGVEPIVLMNDAVTGGGYATI
                     GTVISADRDKLAQTKTNDKTRFRSVDLDEALTAREERRERMAEIRQALS"
     gene            2365..3138
                     /locus_tag="VFQ10_05910"
     CDS             2365..3138
                     /locus_tag="VFQ10_05910"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HET9964909.1"
                     /translation="MQIDISCDMGESFGPWKMGFDEEMMQYISSANIAGGFHAGDPTT
                     MRKTVSLAKDYGVAVGVHPGYRDLVGFGRREMDAPPDEIHDEIIYQLGALREFARYYD
                     MEVQHVKPHGALYMVAARDEDLSRAIIEAIQRIDPGLLLFCMQASVTYQLAQKMDQPV
                     VAEFYADREYNDEGQIVFTRSVAEQLDPEEVGDRVVRAVTEKKVKTESGKDIDVASDS
                     VCVHGDTPGAVRLAEVIARKLGENDIEIRPLGRAQQRIS"
     gene            3150..3389
                     /locus_tag="VFQ10_05915"
     CDS             3150..3389
                     /locus_tag="VFQ10_05915"
                     /inference="COORDINATES: protein motif:HMM:NF005457.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HET9964910.1"
                     /translation="MADKAVKAQMPGTFYRRPDPESDVYVEEGDQVSAGDTIGLIEVM
                     KSFHDVKAEEDGTISKFLVENEEAVDAGQDLVEME"
     gene            3508..>4472
                     /locus_tag="VFQ10_05920"
     CDS             3508..>4472
                     /locus_tag="VFQ10_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9964911.1"
                     /translation="MTEAARRYMGNSVPRKEDTTLLTGRTNFTDDIRLPGMLHMAFLR
                     SPYAHARITSIDASAAREQPGVVAVFTSGDLADVLEGSAMDADSAPGYAVDVEESPRS
                     EADVTPNGGEESADVSESQPRTFCGWPVTENIKIPHHSPLARGEVNFAGEPVAVVLAT
                     DRYKAQDAVEFIEVDYEPMSPATDIEGALEDGAPQVHEEFGTNECYVWSLATGDIDEA
                     FEKADVVVRERYVQQRLIPSAIETRAVVAQPEPLDSFTVYTSTQVPHFVKDVLSVMCG
                     VPDTKLRVVAPDVGGGFGSKLNVYSEEVLALEVARKLGAPVKWVE"
BASE COUNT          931 a         1315 c         1474 g          752 t
ORIGIN      
        1 gggtggcatt ggccgcggtg tgggcggcga ggccagctat tgcggcggct agtgggtacg
       61 agaggaggta gcctcccgtc gggccgagga cgacgccgag cccgccggag aactgggcga
      121 agacgggaac accgaccgcc ccaaccagca cgtagatcgc ctgcgccagc gccctgtatc
      181 ttgacccgag caagagcccg gagaggatga ccgcgagaac ttgcagggtg aaagggtctg
      241 gggagaaggg cagcggtatg gctatctgtg ccgctaccgc tgtcaccgcg gccattagcg
      301 ccgtccgggt catcatcgcg acgttcaagt gcatcttact ctttcgcgca gaggtctggc
      361 tggccccgtc tgttgttggc taatctgcct tttaagaaca cattgaccgg aatttccggc
      421 gctagtagta tgccggcact gtccagagaa gtggagtgat aaggacatca tggaccgcag
      481 catcgagacc gagttgcctc aggcacggta cagctacggt ggggacgagt acgtcttcgt
      541 cgagctcgca gaagcgatga gcttgaaggt gaatttccgg gcaatggcga tcacgaatag
      601 gctcagggag gaggacatcc ccggcgtaac ggacatctgt ccgtcgaacg cctcgtacat
      661 gattcgcata gacccagacg agcttcaccc ggacgatctg atctcgcgcc tcaaggagat
      721 agacgaggag gtcggtgacg ttcacggttt tgaactcaag accaggatcg tggatgtgcc
      781 ggtgatgatc gaagatccct ggacccacga gaccctgatg cgcttccgcg ataggcacca
      841 ggacccgaat gccaccgacc tcgagtattc ggcccgcata aacggcttcg actcgaagga
      901 aggtttcatc gaggcgctcc tgggctctcc gtggctggtg accatgatcg ggttcgtgcc
      961 cgggctcccc tggtgctacc agctcgtccc gccggaggag cagatcgagg ttcccaagta
     1021 cgtgaggccc cgcacctaca cgcccgagcg agccttcggc ttcggagggg gtttcgcggt
     1081 ggtttactcc gtcgaagggg caggcggcta ccagctcttt ggtcgatccc ctgtcccagt
     1141 gctcgacgtg cagcagcgac tgaaggattt ccgggactcc attgtcttcc ccaagcccgg
     1201 cgacgtctgg aactaccggc gcatagaccg cgaggagttc gaccggctcg aggtcgaggt
     1261 cgaggaggga accttcgagt acaggaagaa agacttcgtc ttccacccgg atcgcgcgct
     1321 cgagaaccca catgcctaca acgatgagat actgggggtg ctctatggcg attagggtga
     1381 gacagccagg cctcctgacc acggtccagg acacgggacg tttcggggag tacgccctcg
     1441 ggatgccacc ttcgggggcg atggacgtct tctcattcca ggtaggcaac tacctggtgg
     1501 gcaacgagga tggcgcggct ggcttggaga tcacgtactt cggtcccgag ctggagttca
     1561 ccgaggacac cgtcatcgcg atcactgggg cggagatgcc gccgaagata aacggtgaag
     1621 aggctccgac ctgggagacg cttcgggtgg aggagggcga cgtgctctcc ttcgatgact
     1681 tgaagaacgg ggcccgttct tatctggcgg tagcgggagg gatagacgta cccatcttca
     1741 tgcacagccg gtcgacgtac accctgatcg ggctaggcgg tcacgaggga cgtgcgctgc
     1801 aggagggcga cgaactaaaa gtcggcgagg ctccccgaaa tgccgaaggg caggtgggca
     1861 agagggcgga cgacgaccat atccccacct accccaggga ggcagagctg cgcgtgatca
     1921 tcggcctggc cagctaccgc atcaccgaag agagtatgga cgagttcttg agtatccagt
     1981 ggacggtgac cacggacgca gaccgcgtcg gctaccgata tcagggtggc gaactcgcgt
     2041 tcgttgagcg ggagcagccg gctggggcag gagccgaccc ggcgaacgtc gtggacttcg
     2101 gctatcctgt aggctctatc caggtacccg gcggggtcga gccgattgtg ctgatgaacg
     2161 acgccgtcac cggtggcggc tacgcgacaa tcggcaccgt tataagcgca gaccgcgaca
     2221 agctggccca gaccaaaact aatgacaaaa cccgcttccg ctccgtggat ctcgacgagg
     2281 ccctcacggc ccgcgaggaa cgtcgggagc ggatggcgga gatccggcaa gcattgagct
     2341 aggccggaaa ggtaggttag cgccgtgcag atagacatca gttgcgacat gggggaaagc
     2401 ttcgggccct ggaagatggg cttcgacgag gagatgatgc aatacatctc ctccgccaac
     2461 atcgcgggcg gcttccacgc cggcgaccca accacgatgc gcaagaccgt ctcgctggct
     2521 aaggactacg gtgtcgcggt tggcgtgcat ccggggtacc gggatctagt cggcttcggc
     2581 cgcagggaga tggacgctcc accggacgag atccacgacg agatcattta tcagctcggt
     2641 gctctgcgcg agttcgcccg ctactacgac atggaggtcc aacacgtaaa gccccacggc
     2701 gctctttaca tggtcgccgc ccgcgacgag gacctctcgc gcgcgatcat cgaggcgata
     2761 cagaggatcg accccggcct attgctcttc tgcatgcagg cctccgtgac ctaccagctc
     2821 gcgcagaaga tggaccagcc ggtggtggcg gagttctacg ccgaccgcga gtacaacgac
     2881 gaaggccaga tcgtctttac tcgcagcgta gccgagcaac tcgaccccga ggaggtcggc
     2941 gaccgggtcg tccgggccgt caccgagaag aaggtgaaga ccgagagcgg caaggatata
     3001 gatgtggcct ccgattcggt ctgcgtccac ggcgacactc ccggtgccgt aaggctggcc
     3061 gaggtgatcg cgcggaagct aggtgagaac gacatcgaga tccgtcccct cggacgcgcc
     3121 caacaaagga tctcttagaa ggagcgacat tggctgacaa ggccgtcaaa gcgcagatgc
     3181 ctggcacctt ctaccgccgc cccgacccgg agagcgacgt ctacgtggaa gagggcgacc
     3241 aggtctccgc gggcgacacc atcggcctga tcgaggtcat gaaatccttc cacgacgtca
     3301 aggcggaaga ggatggcacg atctccaaat ttctcgttga gaacgaggag gccgtagacg
     3361 caggacagga tctcgtagag atggagtagg atgccgggac acgcctccgt aaccgcccgt
     3421 tatcaccaga tcaagggact tcgcggtgca caatagaaat ggctgctact ggttaaagga
     3481 ttccgacttt aaggaggagg tcgcgacatg acggaggccg ctcgacggta catggggaac
     3541 agcgtcccgc gcaaggagga cactacgctc ctgacgggac gcaccaactt caccgacgac
     3601 atccgactgc cagggatgct gcacatggcc tttttgcgaa gcccctacgc tcacgccagg
     3661 atcacctcca tagacgcctc ggccgccaga gaacaacccg gcgtcgtggc cgtctttacc
     3721 agcggggacc tggccgacgt gttggagggt agcgcgatgg atgccgatag cgctcccggc
     3781 tacgcagtcg acgtcgagga atcgccccgg agcgaggcag atgtcacccc aaatggtggc
     3841 gaagagtccg ccgatgtatc cgagtcccag ccgaggacct tctgcggctg gccggtcacg
     3901 gagaacatca agatcccgca ccactcgccc ctggcccgcg gcgaggtcaa cttcgccggg
     3961 gagcccgtgg ccgtcgtttt ggcgaccgac cgctacaagg ctcaagacgc cgtcgagttc
     4021 atcgaggtcg actacgagcc tatgagcccg gcgacggaca tcgagggtgc gctcgaagac
     4081 ggagctcccc aggtgcacga ggagttcggg accaacgagt gctacgtctg gtcgctcgcg
     4141 acgggagaca tagacgaggc cttcgagaag gcggatgtgg tggtcaggga acgctacgtt
     4201 caacagcgcc tcatccccag cgccatcgag acacgggccg tcgtcgcaca gcccgagccg
     4261 ctggatagct ttaccgtgta cacctcgacg caggttcccc acttcgtcaa ggacgtgctc
     4321 tccgtgatgt gcggggtgcc ggataccaag ctacgggtcg tggcaccgga cgtgggaggg
     4381 ggtttcggtt ccaagctgaa cgtatactcg gaagaggtgc tggcgctgga ggtggcgcgc
     4441 aagctcgggg caccagtgaa gtgggtggag ga
//
LOCUS       DASTKM010000139         8620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_739885, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000139 DASTKM010000000
VERSION     DASTKM010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739885"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFQ10_05925"
     CDS             <1..906
                     /locus_tag="VFQ10_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HET9964912.1"
                     /translation="PGRFDRRVVVNLPDKVGREAILKVHTRSVPLARSVNLGELAAST
                     PGLSGADLRNLVNEAALLAARREQEEVRQKDFLDALEKIVLGPERPLLMSPEERERVA
                     YHEGGHAILGLVVPGADPVNRVTIVPRGQALGVTYQRPEDDRYNYPEGYLRARIVGAL
                     GGRSAEEIVYGTRTTGAENDIEQATNLARAMVTRWGMSEKLGTVQLAPRENPFLGGGG
                     YPGEKPFSEETARAIDEEVSRIIDESHEEARRLLTRHRKELEALAGALVERETLDEGE
                     ILEVTGLPPAPPLENSKVPDADSRA"
     gene            complement(1303..1767)
                     /locus_tag="VFQ10_05930"
     CDS             complement(1303..1767)
                     /locus_tag="VFQ10_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9964913.1"
                     /translation="MFPTKILLATDGSEDAKLAARTAADLAEKTDSELHIVYVGGEGF
                     YEIPLVDLATLEPTWIAREYPDLLSDIERTERELLDNEVEQLEAAEGTITQAHLSMGE
                     AAPEIVVVAEEIDAGLIVMGSTGQGSIRRALLGSVSESVVKHAHCAVLIVRE"
     gene            complement(2444..3055)
                     /locus_tag="VFQ10_05935"
     CDS             complement(2444..3055)
                     /locus_tag="VFQ10_05935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET9964914.1"
                     /translation="MDHRDFRILGLAASLRRASFHRGIVRAAHEVAPEWMSCEGFDLA
                     RIPYFNQDIEDQGIPESVKELREKIRDYDAVLIATPEYDYAIPGILTSALDWALRSRY
                     DPTPFRHKPVGIVGASPGGGGTIRGQMVLRQILLHPPAYVMPEPQMLIPFSRQKFDVE
                     TGNLVDEETRERLRRFLSTLAAWTQRLEAPLEAHLRQTRERRA"
     gene            complement(3330..5219)
                     /gene="nhaA"
                     /locus_tag="VFQ10_05940"
     CDS             complement(3330..5219)
                     /gene="nhaA"
                     /locus_tag="VFQ10_05940"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET9964915.1"
                     /translation="MTTPRPGQPFSGRTIWIRKLKTPLREFLRTETGGASVLLFAAIA
                     ALVWVNVDASSYDALWATTLSIDIGGAGVALDLRHWVNSGLMTFFFFVLGLEARREFD
                     LGELRERRRFALPLLAAIGGMATAVAIYLAFNMGAPSAHGWGIAMSTDTAFALGLLAL
                     VGRRLPDRLRAFILTLVVVDDLLALVVIATVYTEQVKVSALLAALSVFGAVLVVRALR
                     VHRGLVYAVLGAATWVALLESGVEPVVVGLAMGLLAYAYPAARSDLERAGDRFREFRE
                     QPTPELERSAREELRSAISPNERLQLLYHPWTSYVIVPLFALANAGIAIDGGFLERAF
                     SSPITLGILFGYVAGKPLGILGASWLVTRLSGERLRPPVGWAAVAGGGTIAGIGFTVS
                     LLVATLAFDGLQLEEAKLGILSAALCASIITWLLVRATALLPRRARIHALLGTDEPLV
                     DLYMEVDTERDHIRGPIESPVTIVEYGDFQCPYCGQAEPFVRELLREMGDVRYVWRHL
                     PLSDVHPQAQLAAEAAEAAAEQGAFWEMHDLLLDHQDALQPADLIRYAERLGLGMERF
                     SSELREHTGANRVAEDVNSADLSGVSGTPTFFINGRRHYGAYDTATLAAAVRAAKARA
                     TVAAS"
     gene            complement(5512..5790)
                     /locus_tag="VFQ10_05945"
     CDS             complement(5512..5790)
                     /locus_tag="VFQ10_05945"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HET9964916.1"
                     /translation="MRRLLRDNGLSIAMFGLFFVFVSAQSVMGLLDYNSTQEEHGQPP
                     VGYVQYLGSGGFVEAIFENWESIGVRIRALAISRFRVASPGSCHPEQA"
     gene            5902..6171
                     /locus_tag="VFQ10_05950"
     CDS             5902..6171
                     /locus_tag="VFQ10_05950"
                     /inference="COORDINATES: protein motif:HMM:NF017917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HET9964917.1"
                     /translation="MAEPLWGYRKSVVEEPQDMRSEMAYKQITQLPDNVKNNLPKHAQ
                     VIYKEAFYSAEEQYGEEDRAHRVAWSAVENKYEKNEKGNWVEKDS"
     gene            complement(6435..7463)
                     /locus_tag="VFQ10_05955"
     CDS             complement(6435..7463)
                     /locus_tag="VFQ10_05955"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9964918.1"
                     /translation="MQGVTASSAVICWVSQHPGSGVVEYGKTPELGRKETDPRVRRRH
                     VVALAGLDPGSTYHYRVEGVGGSSPTGCFRTAPVDDDSRFSFAVVGDSGSGGKGQLAV
                     AALLERLRPDLVLHTGDVVYPAGLERHYDRRFFAPYRNLIKTVPLFPVLGNHDVRKGN
                     GAAFLENFHPPLGSPGSTKRYYSFDWGNTHFVALDSELYHGDRGSNPEEQRDFLERDL
                     ATTRKRWKVAFLHRSPYGSSRHGGDEKVRQDLEPLFVKHGVDLVFSGHDHVYERTVPI
                     TGVTYVVSGGGGRRLYPAGNGELTASSVSAHHAVLVRVSGSRLLLETLEVGGKVVDRL
                     ELYQPHAK"
     gene            complement(7641..8069)
                     /locus_tag="VFQ10_05960"
     CDS             complement(7641..8069)
                     /locus_tag="VFQ10_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964919.1"
                     /translation="MKTATTGRSISCETSILTVDRALEQTFSRYAIDPVRLAIGGFSD
                     GASYALSLGITNGDLFTHVLAFSPGLMAPAGRTGSPRIFVSHGMRDGVLPIDCCSRSI
                     VPELKRGGYDMLYREFDGEHSISLEIALEAIGWFTQQKFP"
     gene            8023..8439
                     /locus_tag="VFQ10_05965"
     CDS             8023..8439
                     /locus_tag="VFQ10_05965"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9964920.1"
                     /translation="MEVSQEIDRPVVAVFKFFADDHVRNHPRWDPDIELWRHSDEPIG
                     LGTIIQRRNSRSGTTPVEGTMEVVEYEPNRAFRAVIREGGMETDRRTTFEEVSEGRTR
                     LTIRAETPIDESMKERMSGLVQRSIRNIKELIEAET"
BASE COUNT         1730 a         2658 c         2668 g         1564 t
ORIGIN      
        1 cccgggcgct tcgaccggcg cgtggtggtg aacctcccgg acaaggtggg ccgcgaggcg
       61 atcctcaagg tccataccag aagcgtgccc ctcgcccgta gcgtgaacct gggggagctg
      121 gcggcatcga cgcccgggct ctcgggagcc gacctcagga acctggtcaa cgaagctgct
      181 ctcctggcgg cgcgccgtga gcaggaggag gtgcgtcaga aggacttcct ggatgctctg
      241 gagaagatcg tgctaggccc agagcgtccg ctcttgatga gccccgagga gcgggagcgc
      301 gtcgcctacc acgagggcgg tcacgccatc ctgggcctcg tcgtgccagg agcggacccc
      361 gtaaaccggg tgacgatcgt gccgcggggg caggcccttg gggtcaccta ccagcgcccc
      421 gaggacgacc gctacaacta cccggaagga tacctgaggg cgaggatcgt aggcgctctg
      481 ggagggcgca gcgcggagga gatcgtctac ggcaccagga ccacaggggc cgagaacgac
      541 atcgagcagg ccaccaacct cgctcgtgcg atggtcacgc ggtgggggat gagcgagaag
      601 ctgggaactg tccaactcgc cccgcgcgag aaccctttcc tggggggagg aggctatccg
      661 ggcgagaaac cctttagcga ggaaaccgct cgcgccatag acgaagaggt atcgcgcatc
      721 atcgatgaga gccacgagga ggcccggcgg ctcctcacga ggcatcgcaa ggagctcgag
      781 gcgctcgccg gggccctggt ggagcgggag accctggacg aaggggagat cctggaggtt
      841 acgggtctcc cgcccgcgcc cccattggag aacagcaagg tcccggacgc cgactcccgc
      901 gcgtaggcga gagacccggg atcgcgtggt cgtagaaatt ggtcacgttg gggttcccct
      961 ctacagtctc actatatacg atgctattcc tttgaagcca tactggcatc gcctgttcac
     1021 agcccttcgg taggatcaac gctatgacat acgggggtag atacgcggct atctcgtgag
     1081 ggtcaagctg aagctacatg ggctaagtgt gctctcattg gctatctgac aaaactccat
     1141 aaagccttgt cgggcggtgc tttcatcgaa gtagaggaag aagtaaccgt actcatcagg
     1201 ttgcgccatt tacctaactc cttctctact tcaaccagcg tagatagaat cctggtctcc
     1261 cgcaccgtgc ctttgttctc tgctcctggc gctattctga gcctactcgc gtactatcaa
     1321 gacagcacag tgggcgtgtt tgacaacaga ttcggagacg ctgcccaaca aggctcttct
     1381 gatactaccc tggcccgtgc tgcccatcac tatcagccca gcgtctatct cctcggcaac
     1441 aacgactatc tccggtgcag cctctcccat actcaggtgc gcttgtgtga tcgtcccttc
     1501 agctgcttct aactgctcga cttcgttgtc gagcagctct cgctctgtcc gctcgatatc
     1561 cgacaggagg tccggatact cccgcgcgat ccaagtcggt tccaacgtgg cgagatcgac
     1621 gaggggtatt tcgtagaagc cctccccgcc gacgtagaca atgtgcaact ccgagtcagt
     1681 cttctccgcc aggtccgccg cggtccgcgc cgctagcttc gcatcttctg aaccgtcagt
     1741 tgccaagagg atcttggtcg gaaacatgct catcgttcgc ctcctcgccc ggagttctac
     1801 ccaagagcac atgatgccag cgggcgaacg tcagcgcaca agatacgcgc cggtttcctc
     1861 aaatacagaa aggattggag cgccacctag cctccagcct attcagggct gctgaaagcc
     1921 ttagcctgtc tgaagtacaa acaagtatgg gcaagataaa gcatctgacg gccttcggga
     1981 gcgaagcccg gtagttccga tcatattctt gacaacagtt gacgacaacc catgcgaaca
     2041 acggcgaata tcgaggaggg aatggggtcc gaaggttgcg gacttgacgc cacgctgggg
     2101 ccaccgacgg cacaatactc cgcaacgcct tatttatcac tcgcaatgag atgaggtcag
     2161 cagttcgagt ccgctcgtcg actttcccaa atcagcttac gtaagcgcaa tactcgcacg
     2221 agttatgact cccgtcggtg agtcgggagc cttttgacat cacgtggtct cgcatacgaa
     2281 cgttttgaca ggaacgccca cgcccgtaaa gcccttccca agctgctcct cgacgttgcc
     2341 gattgctcca attatcagga tggtgtgagc tactcagatc caaccggctc agtgcgtccc
     2401 tgtggagggt atgactaacg agagctgctc gcggttgcgc ccatcaggct ctcctctcgc
     2461 gagtctgcct caagtgagcc tccaatggtg cttctaaccg ctgagtccac gctgccagcg
     2521 tcgagaggaa gcggcgcagc ctctcgcggg tctcttcatc cacgaggttg ccggtctcca
     2581 catcgaactt ctggcgcgag aagggaatca gcatctgagg ctcgggcatc acgtaggccg
     2641 gaggatgcaa gagtatttgt cttaagacca tctgaccccg gatagtccca ccacctcccg
     2701 ggcttgctcc cacgatcccg accggcttgt gtcggaaggg cgtgggatcg tagcgtgagc
     2761 gcagcgccca gtccagggcg gaggtcagga tgccgggaat ggcgtagtcg tactcaggtg
     2821 ttgctatgag cacggcatcg tagtcgcgta tcttctccct gagctctttc accgactcag
     2881 ggatgccctg gtcctctatg tcctggttga agtacggtat gcgggcgagg tcgaagccct
     2941 cgcaactcat ccactccgga gccacctcgt gcgccgccct cacgatgcca cggtgaaagg
     3001 aggctcgcct gaggctagcg gcgaggccga gtatgcggaa atctctatga tccattgctt
     3061 gtcctccgtt ggcgttctcg atcccgacca cctttcaagt cggtccatca tagctcgcga
     3121 tagccaacac ctgcacccac agggtaggcc cgctcaacga gggcgaacgc gacggcgacg
     3181 tggttacctg tccctggcac ggctcttagt tcgacctgtg ctccaggaaa gtgttgcggg
     3241 gacctgccag agaccccagc gatgcttcga gacccgggtg cgtgtaggga agatagaggt
     3301 acgcacggag tactagatca ggtagacggt catgacgcgg ctaccgtcgc cctcgccttg
     3361 gccgcacgga cggcggccgc aagggtggcg gtgtcgtaag cgccgtagtg acgccggccg
     3421 ttgatgaaga aggtaggggt tcctgagacc ccgctaaggt cggcactgtt gacgtcctcg
     3481 gcgactcggt tcgcacccgt atgctcgcgc agctcgctgg agaagcgctc cataccgagg
     3541 ccgagccgct ccgcgtagcg gatgagatcg gctggttgga gtgcgtcctg gtggtcgagc
     3601 agcaggtcgt gcatctccca gaacgcgccc tgttcggcgg ctgcctcggc tgcctcggca
     3661 gcgagttggg cctggggatg gacatcgctc agcggcaggt ggcgccagac gtagcggacg
     3721 tcgcccatct cgcgcaggag ctctcggacg aacggctcgg cctggccaca atagggacat
     3781 tggaagtcgc catactcgac gatcgtgacg ggtgattcga tgggcccgcg gatgtggtcg
     3841 cgttccgtat cgacctccat gtagagatcg acgagcggct cgtcggtgcc gagcagggcg
     3901 tgtatccggg cgcgccgggg gagcagcgct gtggcgcgaa ccaggagcca cgtgatgatc
     3961 gacgcgcaaa gggcggcgct aaggatgccg agcttcgcct cctccagctg cagcccgtcg
     4021 aaggcgagcg ttgcgactag cagcgacacc gtgaagccga ttccagcgat cgtgccgccg
     4081 ccggctacgg ccgcccagcc gaccggcggc cgtagccgct cgccgctcag tcgcgtcaca
     4141 agccacgacg cgcccagaat gccaagcggc ttgccggcga catagccgaa caggatcccc
     4201 agcgtgatcg gcgagctgaa ggctcgctcc aggaagccgc cgtcgatcgc gattcccgca
     4261 ttcgcgagcg cgaacagcgg aactatcacg tagctggtcc acggatggta gagcagctgt
     4321 agccgctcgt tcggcgagat cgccgacctc agctcctcgc gcgcggagcg ctccagttct
     4381 ggcgtcggtt gctcgcggaa ctcccgaaag cgatcgccag cgcgctcgag atcggaccgc
     4441 gccgccgggt aggcgtaagc caggaggccc atcgcgaggc ccacgaccac cggctccacg
     4501 cccgactcga gcagcgctac ccaggtcgcc gccccgagca ccgcgtagac caggccccga
     4561 tgcacgcgga gggcgcgcac gacgaggaca gctccgaaga cactcagggc ggcgagcagc
     4621 gccgaaacct tgacctgctc tgtgtagacc gttgcgatga cgacgagcgc gaggaggtcg
     4681 tcgaccacga ccagcgtaag gatgaacgcc cgcagacggt caggaagacg ccgccccacg
     4741 agcgcgagca ggccgagcgc gaaggccgtg tcggtcgaca tcgcgatgcc ccagccgtgc
     4801 gcggacggag cacccatgtt gaaggcgagg tagatggcga ccgccgttgc catcccgccg
     4861 atcgcggcca gcagcggcag cgcgaaccgt cgtctctctc gcagctcgcc caggtcgaac
     4921 tcgcgccgtg cctcgagccc cagcacgaag aagaagaacg tcatcagccc gctgttcacc
     4981 cagtggcgaa gatcgagcgc gacaccagcc ccgccgatgt cgatcgagag cgtcgtcgcc
     5041 cagagcgcgt cgtaggagga cgcgtcgacg ttgacccaca cgagcgcagc tatcgccgca
     5101 aagagcagga ccgacgcgcc gccggtctcc gtgcgcagaa actcccgtag aggggtcttg
     5161 agcttccgaa tccagatcgt acgccccgag aacggctgac ctggccgcgg cgtcgtcacg
     5221 ctctgctccc atgccagcgc tgacggcggg attcgagtac tgccctgcac cccgggatca
     5281 gcttcgggtg atccatcttt gaagtgtact agttcatttt cgtagtcgag atggaagctt
     5341 ctgcgctcct tgtaaggaag gaggagagct ggcagaatcc cagattgtga tcctcagagt
     5401 agctcgagca cgtacttatg ttcgcgtacc tctttgccgc cttcagcttc aaaaccgact
     5461 taagcaaagg gctgtcccaa gagcagctaa aagcggtcat aaggtggaat gctacgcttg
     5521 ctcagggtgg caggaaccag gagaggccac acgaaaacgt gagatcgcca gcgcccgaat
     5581 tctgacgccg attgactccc agttctcgaa aatggcttcg acgaagcccc cgcttcctag
     5641 gtactgaacg tagccgaccg gaggctgccc atgctcctct tgggtgctgt tgtagtccaa
     5701 caagcccata acactctgcg cgcttacaaa gacgaagaag aggccgaaca tggcgatcga
     5761 aagcccatta tcgcgcagca ggcgtcgcat taagttcctc cgttcggtgt cctatccttg
     5821 gctggtaggt ctccaataag cttcgcgaga cccgcccagg ttccatcata ctattgctct
     5881 ttcttaccag cgtacaagag gttggccgag cccctttggg ggtacagaaa gagcgtagta
     5941 gaagagccac aagatatgag gagcgagatg gcctacaagc agatcactca gctaccggac
     6001 aacgtgaaga acaacctgcc caagcacgca caagtgatct acaaagaggc cttctactcc
     6061 gcagaggagc agtacggcga ggaggaccgc gcccataggg tcgcctggag cgccgtagag
     6121 aacaagtacg agaagaacga gaaaggcaac tgggttgaga aggatagcta actcgtgctg
     6181 caggggtgcc gggaccctag ttttatcgcg tactcacctc cggtcgctgc gccattcggc
     6241 ggcactaggt ctaggctgtt cgttctccct ctgccgggcg ttcgcctatt tcgggcagct
     6301 ctcgaaccgg cgttagggct tggccgtaga tcctccagtc gaggtcgcgg gcgcgcttta
     6361 taaagaccac acgcctctta gggttactgg gacgtatact tgaagcggtt gcgagccgac
     6421 tcttcgcgag acggtcactt cgcatggggc tggtatagtt ccaagcggtc aacgaccttg
     6481 ccgcccactt ctagggtctc tagcaacagg cggctgccgc tgacgcgcac gagcaccgcg
     6541 tggtgggcgg acacggagga ggccgtcaac tcgccattgc ccgccggata cagcctcctg
     6601 ccgcctcccc cgctgaccac gtaggtcact cccgttatgg gcacggtgcg ctcgtagacg
     6661 tggtcgtggc cagagaagac gaggtccacc ccatgcttca cgaagagcgg ctctaggtct
     6721 tgcctcactt tctcgtcacc gccgtgcctc gaggagccat agggagacct atgcaagaac
     6781 gcgaccttcc agcgcttgcg cgtggtggcc aggtctcgct ccaggaagtc cctctgctcc
     6841 tccgggttag aacccctatc accgtgatac agttcgctgt cgagcgcgac gaagtgggtg
     6901 ttgccccagt cgaaggaata gtagcgcttg gtgctacccg gactgccgag gggcggatgg
     6961 aagttttcga ggaaggctgc gccgttcccc ttcctcacgt cgtggttgcc gaggacaggg
     7021 aagaggggta ccgtcttgat gaggtttcgg tacggagcga agaagcggcg gtcgtagtgg
     7081 cgctccaggc ctgccgggta gactacgtcg cccgtgtgca ggactaggtc cggcctcagg
     7141 cgttccagca gcgcggccac tgctagctgt ccctttcctc cgctgccact gtcgcccacc
     7201 acagcgaagc tgaatcgtga gtcgtcgtcc acgggggcgg tgcggaagca ccccgtcggc
     7261 gacgacccgc caacgccttc cacacggtaa tggtaagtcg agccggggtc gagaccggca
     7321 agcgcgacca cgtggcgcct gcggactcgg gggtctgttt ccttacgccc cagttcgggg
     7381 gtcttgccgt actcgaccac gcccgatccc gggtgctggc tcacccagca gatgactgcc
     7441 gaggacgccg tgacgccttg cacgtagggc tctcgcggct gttctaaccc tgtggtacca
     7501 ccgagcagag accgcgcggt ccgcagcgct agtgcgaacc gccctcgttc gaagaagctc
     7561 gagatcattt caccaatgct acccggagtt gcgggcggag cgcgaggagg tccgctcccg
     7621 gaacggcgct gaccggtcct ttaggggaac ttctgttgcg taaaccagcc gatcgcctct
     7681 agggcgatct cgagcgagat cgagtgctcc ccgtcgaact cccggtagag catatcgtag
     7741 ccgccacgct tcagctcggg gacgatgctg cgactgcagc agtctatcgg aagcaccccg
     7801 tcgcgcatgc cgtgggagac aaatatgcgc ggggagccgg tccgcccggc cggggccatg
     7861 agccccggcg agaacgcgag cacgtgcgtg aagaggtccc cgttggtgat ccccaaagag
     7921 agcgcgtagg aggccccatc cgagaaaccc ccgatggcca gacgcaccgg atcgatggcg
     7981 tagcgggaaa acgtctgctc aagcgcccgg tcaacagtta ggattgaggt ctcacaagag
     8041 atcgatcgtc ctgttgtcgc tgtcttcaag ttctttgccg acgaccatgt tcgtaaccac
     8101 ccgcgttggg acccagatat cgaactgtgg cgtcactcag atgaacccat cggcctcgga
     8161 acgatcatcc agaggcgcaa tagccgttcg ggcactactc cggtggaagg cacaatggaa
     8221 gttgtggagt atgagcccaa cagggctttt agggccgtca ttcgagaggg gggtatggag
     8281 actgacagaa ggaccacctt cgaggaggtc agtgagggcc gcacgcgact aacgattcgt
     8341 gccgagacgc cgatcgatga atcgatgaag gaacgcatga gcggcctggt gcagcggagc
     8401 attcgaaaca tcaaggaact catagaggca gaaacctagc tgcaacgtgc tgtaaatagc
     8461 cctattcacc caagtgatcc atggcccttg ccgggccacc ccgaagccat gaaaatggag
     8521 ctgccgccga ggctgtattc acaccagtgc ctggaagagg tcttctcgga agttcgccgt
     8581 attcaagtta ccattctgaa ttcataggta ttttcgagga
//
LOCUS       DASTKM010000140         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_739934, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000140 DASTKM010000000
VERSION     DASTKM010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739934"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VFQ10_05970"
     CDS             complement(<1..1049)
                     /locus_tag="VFQ10_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964921.1"
                     /translation="MKVAFIGAGSVVFTKNLLTDTLSFPELHGTTIALHDIDPERLET
                     AGMMARWTSDQLDADASVEEYLDRRRALEGADFVVNMVQIGMHEATLLDFEIPKKYGL
                     KQTIADSMGIGGIFRGLRTIPFVLDLAGEMRELCPNALLLNYTNPMGILMEALYTASP
                     ETKSVGLCHSVPYTAREISSYIGVEHEELLYECAGINHIAWMTRLEVEGEDAYPRLFA
                     AMEDPDVYAKDKVRFELMRRFGYFVTESSEHNAEYVPYFLKDDELIQLYDVPVDEYVR
                     RSEENLVQYAETRRKLLAGEDFSLGRSVEYGSLIMHSLVTGEARAIYGNVENTGLVTN
                     LPEASCVEVPILVDGT"
     gene            complement(1298..2833)
                     /locus_tag="VFQ10_05975"
     CDS             complement(1298..2833)
                     /locus_tag="VFQ10_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HET9964922.1"
                     /translation="MRTKDAYIDTHLRESLVEENSFVRMPPEDIPPPSFEDAWDSLPQ
                     PFWGGHEGAIDCYRRAWELAFSNLRRPEPESGFVASYIDTAFNDCLFMWDSAFILLFG
                     RYGRRAFAFHRTLDNLYAKQHPDGFICREIGTLDGKDRFERFDAASTGPNVMPWTEWE
                     HYVDTADRERLGRVFPVLVSYHRWLRRHRTWRDGTYWTSGLGSGMDNQPRFPQRPAGK
                     VGWPERLTYHGHAVWIDACLQQVLSADLLLRMADALCRTKEVADLREERDRLSMTVNE
                     RLWNEETAFYHDELADGTLSDVQTVGAFWALLAGVVPQERISSFVGHLEDPKRFGRPH
                     RVPSLSADHPEYDAEGGYWLGGVWPPTNCMVLRGLDWAGYNDLAHEISREDLDYVVAV
                     FEQTGTLWENYAPERTAPGNRAKDDFVGWSGLAPVAGLFEYVFGLRADASSGRLLWDV
                     RLLEDHGVHGYPFGRDGVLELSCANRNSATVEPEIQVRSTVPLELMIRWEGGEKTIAV
                     PES"
BASE COUNT          518 a          888 c          925 g          576 t
ORIGIN      
        1 gtgccgtcga ccaggatcgg cacctcgaca cagcttgctt cggggaggtt cgtaacgagg
       61 cctgtgttct cgacgttgcc gtatatggcc cgcgcctcgc cggtgacgag cgagtgcatg
      121 atgagcgagc cgtactcgac gctgcgaccc agcgagaagt cttctccggc gagcagtttg
      181 cgacgcgtct cagcgtattg gacgaggttc tcctcgctgc gcctgacgta ctcgtcaaca
      241 ggcacgtcgt agagttggat cagctcatcg tccttgagga agtagggtac gtactcggcg
      301 ttgtgctcgc tcgactcggt gacgaagtac ccgaagcggc gcatcagctc gaagcgcacc
      361 ttgtctttgg cgtacacgtc agggtcctcc atcgccgcga acagccgtgg gtaggcgtct
      421 tcgccttcca cctccaagcg tgtcatccag gcgatgtggt tgatgccggc gcactcgtaa
      481 agaagctcct catgctccac gccgatgtac gaggagatct cgcgggcggt gtaagggacg
      541 ctgtggcaga gcccgacgct ctttgtctct gggcttgcgg tgtacagcgc ctccataagg
      601 atgcccatcg ggttcgtgta gttcaggagc agcgcattcg gacagagctc tcgcatctcg
      661 ccagcgaggt cgaggacgaa tgggatagta cgcaggccgc ggaagatgcc gccgatgccc
      721 atcgagtcgg cgatcgtctg cttgaggccg tacttcttgg ggatctcgaa gtcgagcagg
      781 gtggcctcgt gcattccgat ctggaccatg ttcacgacga agtctgctcc ttcgagcgcc
      841 cgcctccggt cgagatattc ctcaacactc gcatcggcgt cgagctgatc cgaggtccag
      901 cgggccatca tgccagcggt ctccaggcgc tcggggtcta tgtcgtgcag ggcgattgtg
      961 gttccgtgca gctcggggaa gctcagggtg tctgtcaata ggttcttggt aaagacgacg
     1021 ctccctgcac cgataaaggc taccttcaag gttaggtcct ttctctgaac gagtaacttg
     1081 cgatcccctc cacacaggtt acttcaatac ggctaccccg gcagggggaa tgtcgatctc
     1141 gtgcaacccg gtctcgaacc cgataggtgg cttctagttg gaggcgcgga gcgctggctg
     1201 acgtagattt ccgcttgcgc gggaatgttg ctctcgagct gggaggattg cgtgcgccca
     1261 agttgtccgc cgcggaaatg atactgcgga ttccaaatca gctctcgggg acggcgatgg
     1321 tcttttctcc accctcccac ctgatcatga gttctagggg taccgtggag cgcacctgga
     1381 tttctggctc tactgtggcg gagttgcggt tggcgcaaga gagttccagg actccatccc
     1441 tgccgaacgg atagccgtgg acgccgtggt cttccaggag ccgtacgtcc cacaggagcc
     1501 gtcctgagga cgcgtcggcg cgcaacccga acacgtactc gaagagtcct gccacgggcg
     1561 cgaggccact ccagcctacg aagtcgtctt tggcacggtt gccaggcgcc gtccgctcag
     1621 gggcgtagtt ctcccagagt gttccggtct gttcgaagac ggctaccacg tagtcgaggt
     1681 cctcgcggga gatctcgtgc gccaggtcgt tgtaaccggc ccagtcgagt ccccgcaaga
     1741 ccatgcagtt cgtgggcggc catacgccac cgagccagta gccgccttcc gcatcgtatt
     1801 ccggatggtc ggcggagagc gagggcacgc gatggggccg cccgaatctc ttggggtcct
     1861 ccaggtgtcc gacgaacgag gagatcctct cctgcggcac aacgcctgcc agcagagccc
     1921 agaaggcgcc gacggtctga acgtccgaga gcgttccgtc cgcaagctcg tcgtggtaga
     1981 aggcggtctc ctcgttccac agacgctcgt tgaccgtcat ggagagccgg tcgcgctctt
     2041 cgcgcaggtc cgcgacctct ttggtgcggc agagggcgtc tgccatgcgc aggagtagat
     2101 ccgccgatag cacctgttgg aggcacgcgt cgatccagac ggcgtgcccg tggtaggtca
     2161 ggcgttcggg ccacccgacc ttccccgccg gtctctgcgg aaagcgcggc tggttatcca
     2221 ttccggagcc gaggccggac gtccagtagg tgccgtcgcg ccaggtgcgg tggcgccgca
     2281 gccacctgtg gtaggagacg agaacgggga agacccggcc taggcgctct cggtcggccg
     2341 tgtcaacgta gtgctcccac tccgtccacg gcataacgtt cggccccgtg ctcgccgcat
     2401 caaagcgctc gaagcggtct ttaccgtcca aggtgccgat ctcccggcag ataaacccgt
     2461 cgggatgctg cttggcgtag aggttatcca gggtgcggtg gaaggcgaag gcacggcggc
     2521 cgtaacggcc gaacagcaag atgaatgccg agtcccacat gaaaaggcag tcgttgaaag
     2581 ccgtgtcgat atagctggcg acgaagccgc tctccggctc gggacggcgc aggttggaga
     2641 atgctagctc ccacgcccta cggtagcaat ctatggcacc ctcgtgtccc ccccagaagg
     2701 gttggggcag ggagtcccaa gcgtcctcga acgagggcgg cgggatgtct tccggaggca
     2761 tccgcacaaa ggagttctcc tcgaccagcg attccctgag atgggtgtcg atgtaagcat
     2821 ccttcgttcg caactcgttg acctcctcta atcgcctgat ccagcatcgc gcatacacca
     2881 tccacgcaca agttcttggt aggcccc
//
LOCUS       DASTKM010000141         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_740044, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000141 DASTKM010000000
VERSION     DASTKM010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740044"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VFQ10_05980"
     CDS             <1..1033
                     /locus_tag="VFQ10_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HET9964923.1"
                     /translation="RTAPSTTPTRTTRRSWGRSMAIKVQSPGLLTTVQDTGRLGEYAI
                     GMPPSGAMDVFSYQIGNYLVGNEEGAAGLEITYFGPRLEFTEDAVVAVTGAKMPPKIN
                     GEEVPTWETLAVESGDVLALDYLQNGARAYLAVAGGIDVPVFMHSRSTYTLIGLGGHE
                     GRALAEGDELALGEAGDRSDRVGMAVVEQQIPHYSKEAELRVIIGLASYRLTEESMEA
                     FLNTTWTVTTDADRVGYRYKGGELGFLEREQPAGAGADQANVVDFGYPVGSIQVPGGV
                     EPIVLMNDAVTGGGYATIGTVISADRDRLAQTKTNDKTRFRSVDLEEALAAREQRRKQ
                     MAEIKEALS"
     gene            1179..1952
                     /locus_tag="VFQ10_05985"
     CDS             1179..1952
                     /locus_tag="VFQ10_05985"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HET9964924.1"
                     /translation="MQIDFNCDMGESFGPYSFGADEELMRHISSANVAGGFHAGDSHV
                     MRETVALAREHGVAVGLHNGYGDLVGFGRREIKAAPEELRDELIYQLGALREFARLYG
                     LEVQHVKPHGALYMAAARDEELSRAIIGAIQESDPALVLYCMQSSKTHEIAQEMGQPV
                     AREFFADREYNDEGQIVFTRRVGEELDPEEVAERVLRGVTEGRVRTVDGNDIEVAADS
                     ICVHSDTPGVARLAETIVRKLGENGVEVRPVGRAEQRVP"
     gene            1970..2206
                     /locus_tag="VFQ10_05990"
     CDS             1970..2206
                     /locus_tag="VFQ10_05990"
                     /inference="COORDINATES: protein motif:HMM:NF005457.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HET9964925.1"
                     /translation="MAKTVKAQMPGTFYRRPDPESGPYVEEGDQISAGDTVGLIEVMK
                     SFHEIKAEEEGTIARFLVENEEAVDAGQDLVEME"
BASE COUNT          470 a          688 c          795 g          362 t
ORIGIN      
        1 ccggaccgcg ccctcgacga cccccacgcg tacaacgagg agatcctggg ggcgctctat
       61 ggcgattaag gtgcagagcc ctggtctact caccaccgtc caggacacgg gccgcctcgg
      121 cgagtacgct atcgggatgc ctcctagcgg agcgatggac gttttctcct accagattgg
      181 caactacctc gtcggcaacg aggaaggcgc ggcgggcctg gagataacgt acttcggccc
      241 taggctggag tttacagagg acgccgtagt cgccgttacg ggggccaaga tgccgccgaa
      301 gatcaacggc gaggaggtcc cgacctggga gacgctcgct gtagagtcgg gggatgtgct
      361 agccctcgac tacctccaga acggtgcgag ggcctacctg gctgtggcgg gcgggataga
      421 cgtgcccgtc ttcatgcaca gccgctcgac ctacaccctg atcgggcttg gaggccacga
      481 gggcagggcg ctggctgagg gcgacgagct ggcgctgggc gaggcgggcg accgctccga
      541 ccgcgtcggg atggccgtcg ttgagcagca gatcccccac tactccaaag aggcggagct
      601 gcgcgtcatt atcgggctcg ccagctatcg gttgaccgag gagagcatgg aggcgttcct
      661 caacaccacg tggacggtca ccactgacgc tgaccgggtc ggctaccgct acaagggcgg
      721 cgagctcggg ttcttggagc gggaacagcc ggctggtgcc ggggcagacc aggccaacgt
      781 ggttgacttc ggctaccccg ttggctccat ccaggtgcct ggcggcgtcg agcccatcgt
      841 cctgatgaac gacgccgtca ccggcggggg ctacgcgacc ataggcaccg tcataagcgc
      901 ggaccgcgac agacttgccc agaccaagac aaacgacaag acccgtttcc gctccgtcga
      961 tctcgaggaa gcgctcgcgg cccgagagca gaggcgcaag cagatggcgg aaatcaagga
     1021 agcactttct tgaggcatca ggagctgtgg gacggttctc ggtggagcga tgcgccgttc
     1081 gggtgagcga caaggtctgt cctgctcgaa gtcaatcgcg aatgcggacc tgatgcacga
     1141 aacctgaagc ctaaagccgc cgaccgaagg gagtcacatt gcagatagac ttcaattgcg
     1201 acatgggtga gagcttcggc ccttatagtt tcggcgcgga cgaggagttg atgcgccaca
     1261 tctcctcggc caatgtcgcc ggcggcttcc acgccggcga ctcgcatgtc atgcgcgaga
     1321 cggtagcgct ggccagagag cacggcgtcg cggtcgggtt gcacaacggg tatggtgacc
     1381 tggttggctt cggcaggagg gagataaagg ccgcgccgga ggaactccgc gacgagttga
     1441 tctaccagct aggtgcgctg cgcgagttcg cccgcctcta cggcctggag gtccagcacg
     1501 tcaagcccca cggcgccctc tacatggccg cagcccgcga cgaggagttg tcgcgcgcca
     1561 tcatcggggc gatccaggag tcagacccgg ccctcgtcct gtactgcatg caatcctcga
     1621 agacccacga gatcgcccag gagatgggcc agcccgtcgc gagggagttc ttcgccgacc
     1681 gcgagtacaa cgacgaaggc cagatcgtct ttacccgtcg cgtaggagag gagctcgatc
     1741 cggaggaggt ggccgagaga gtcctccggg gcgtgacgga gggcagggtc aggaccgtgg
     1801 acggtaacga catagaggtg gctgcggact cgatctgcgt ccacagcgac acccccgggg
     1861 tcgcgaggct ggccgagacg atagtgcgaa agctcgggga gaacggcgtt gaggtaaggc
     1921 cagtcggacg agcggagcag agggttccct agcacgagga gagttcgagt tggcgaagac
     1981 cgtgaaagcc cagatgccag gtaccttcta ccgccgcccc gatccggaga gcggccccta
     2041 cgtcgaggag ggcgaccaga tctcggccgg agataccgtc ggcctgatcg aggtcatgaa
     2101 atcgttccac gagatcaagg ccgaggaaga gggcaccatc gccaggtttc tagtcgagaa
     2161 cgaggaggcc gtggacgcgg gccaggatct cgtagagatg gaataggatg ccgggacacg
     2221 cctccgtaac cgcccgttct cacaaaatca ggggacttcg cggtgctcaa tagaactggt
     2281 tgctactggt caaaggactc cgactcaaag gagga
//
LOCUS       DASTKM010000142         5346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1269840, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000142 DASTKM010000000
VERSION     DASTKM010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269840"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..772)
                     /locus_tag="VFQ10_05995"
     CDS             complement(44..772)
                     /locus_tag="VFQ10_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9964926.1"
                     /translation="MLTSHTTGGFATTSAPQGFDLTDFEEAGLRVVERRFGPRDTIFT
                     PGDPDDQLYFLLSGTVRLYKIYGDYKEATTALLKDGGVFGKLNLVEGRWQDVFAEAVT
                     DSRVASIQKASIERVIKSDPGFALKLFSSFSERLRQSDEVIESLLHREVSTRLATLLI
                     NLGERFGESNGVGTLIDVRLTHQDLANMIASTREAVSKVMSELQRDGVIESRNRRIVI
                     VNGKALAEYASRLSGLSIDGQAAI"
     gene            complement(912..1451)
                     /locus_tag="VFQ10_06000"
     CDS             complement(912..1451)
                     /locus_tag="VFQ10_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964927.1"
                     /translation="MRLTAMRFAESIGEPAERRNVTLGVIQHLWRSPVPFIGFYPPEG
                     VKVTENRLYTPAPPKSVDDTDYLAGVEAHKRAFAKDLENAMVPTGRLPGYFEVPTDEG
                     WRITLELAEGRLGLADAWGMNSEREVAGDLAPFAWAYRNCDFCIVTIYDKDIGALRDD
                     LLFVAKRAGVELRWRRSAA"
     gene            complement(1635..2336)
                     /locus_tag="VFQ10_06005"
     CDS             complement(1635..2336)
                     /locus_tag="VFQ10_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HET9964928.1"
                     /translation="MFIPGGFGGYLLIMIVGMIISGAAALWVRSSYSKYSKKRSASGL
                     TGAQTARMILDRNALTNVRVEPVAGQLTDHYDPRSKVVRLSEDNFRGNSIAGVSVAAH
                     EVGHAIQDATGYVPMKLRAGIFPIVNFSSQIWPLAFIMAFFVGGAASPFGSLLINVAI
                     VLFLGVLLFHVVTLPVEINASTRAYGLLTRYGILSGTEAGGTRRVLTAAAFTYIAAAL
                     TAVLTLLYLLAVSRQ"
     gene            complement(2543..4465)
                     /gene="acs"
                     /locus_tag="VFQ10_06010"
     CDS             complement(2543..4465)
                     /gene="acs"
                     /locus_tag="VFQ10_06010"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET9964929.1"
                     /translation="MAEETKIRTYEPPEEFAREANVKDPSVYEEAEQDYEGFWAERAR
                     ELHWFREWDRVLDWDPPEAQWFVGGKLNVSYNCLDYQVEQGRGDKTAILWQGDEPEDS
                     KTFTYSELKAEVERFANVLKGLGVQKGDPVAIYLPMIPELPIAMLACARIGAPHSVVF
                     GAFSADSLRDRINDCEAKVLVTADGGPRGGKVTPLKANADEALQDTPSIENVVVAQRT
                     GEDVDMVEGRDRYWNDLMAEADAECPAEEMDSEDILYILYSSGSTGKPKGIVHTTGGY
                     LTQVKATTKWVLDVKEDDVYWCQADIGWVTGHSYLVYGPLSNGVTTVQFEGTPSYPGP
                     DRHWDVIERHGVTIYYSAPTVIRAFMKQGTEPIEKHDLSSLRMLGTVGEPINPRAWEW
                     YHENIGGGRCPIVDTWWQTETGGIMIAPLPGITPTKPGSATWPFPGIFVDIYDDDDNP
                     IEGPGKGNLVVTRPWPGMLRTIYKDPERYRKTYWERLGDKYFVEDGAERDEDGYYTIT
                     GRIDDVMNVSGHRIGTMEVESALVAHKAVAESAVIGRDDEDKGQAIVAYVILEGDREG
                     SEELIKELNDQVRKAIGPHARPEEIIFTPDLPKTRSGKIMRRVLRGVAEGEDDLGDVT
                     TLADPSVVDELKEARH"
     gene            4740..>5346
                     /gene="fabI"
                     /locus_tag="VFQ10_06015"
     CDS             4740..>5346
                     /gene="fabI"
                     /locus_tag="VFQ10_06015"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HET9964930.1"
                     /translation="MGGLLEGKKVLVTGVLDRRSIAYSAARLTQEQGAEIILSSFGRA
                     RSLTERTARRLHPEPPILELDVNKPEDIEAVADDLAERWGDLDGIVHAIAFAPEDALG
                     GNFLTAEWPSVATALQTSAYSLKALSVGLLDLLKPGASVVGLDFDARIAWPAYDWMGV
                     AKAALESTSRYLARDLGAHGIRVNLVSAGPVKTLAAKGISGF"
BASE COUNT          940 a         1727 c         1584 g         1095 t
ORIGIN      
        1 cgttgcagaa aaagagctgt ccggaaaggc agcgacaggg aggctagatc gcggcctgcc
       61 cgtcgatgct caggccggat aacctggagg cgtactcggc aagggccttc ccattgacta
      121 taacgattct gcggttgcgg ctctctataa ccccgtcgcg ctggagctcg ctcatcacct
      181 tcgagacggc ctcccttgta gaggcgatca tgttcgccag gtcctggtgg gtgagccgca
      241 cgtctatcag tgtgccgaca ccattgctct ccccaaagcg ctcgcccagg tttatcagca
      301 gcgtcgcgag cctggtcgag acctccctgt gcaacaggct ctctatcacc tcatcggact
      361 gcctgagccg ctcggagaac gaggagaaga gtttgagggc gaagccggga tcgctcttta
      421 tcacccgctc tatggatgcc ttctggatac tcgccacccg cgagtcggta acggcctctg
      481 cgaagacatc ttgccaacgt ccctccacca ggttgagctt gccgaagacg cccccatcct
      541 tgagaagcgc ggtcgtagcc tccttgtagt cgccgtagat cttgtacaga cgcaccgtgc
      601 ccgagagcag gaagtagagt tgatcgtcgg gatcccctgg cgtaaagatc gtgtccctcg
      661 gaccaaaacg cctctctacg accctgagac cggcctcttc gaagtctgtg agatcgaatc
      721 cttgcggtgc gcttgtggta gcgaaccctc cggtggtgtg gctggtgagc atctgacgcg
      781 acctctctaa tggaactctc tgtaaatccg gtgaaaacct ccggacgcgc atattatacc
      841 cgaagttcta atttctattc ttgatctgcg agggcctcct ggcgccgttt ggccaggtcg
      901 ctgagaatcc ctcaggctgc actcctgcgc cacctgagct ccacaccagc acgcttggcg
      961 acgaagagaa ggtcgtcgcg cagggccccg atgtctttgt cgtatatggt gacgatacag
     1021 aagtcacagt tgcggtaggc ccaggcgaag ggcgcgagat ccccggccac ctccctctcg
     1081 gagttcatgc cccaggcgtc agccagcccc aggcgtccct ccgcgagctc cagggttatg
     1141 cgccacccct catcagtcgg gacctcgaag tagccaggca acctccctgt ggggaccata
     1201 gcgttctcca ggtctttcgc gaaggccctc ttgtgggcct cgaccccagc gagatagtcc
     1261 gtgtcgtcca ccgactttgg gggagccggc gtgtagaggc gattctccgt aacctttact
     1321 ccctcaggcg ggtagaaccc gatgaacggg accggcgagc gccagaggtg ctgtattacc
     1381 ccgagagtca cgttgcggcg ctccgcaggc tctccgatag actcggcgaa gcgcatcgcc
     1441 gtcaggcgca tgtgccttgg ctcccacgga tgtggctctc ctacggtccg tagccgcctc
     1501 cggcgcttcg ttatgcgact gacctcttcc atgacgcgcg tattctacaa ctctcgtgcg
     1561 gccgatgccc ggcaaggaaa agttcgaggg cggggccgaa gccccgccct cttggtccgg
     1621 acgaaaggtc cgctctactg cctgctcacg gccagcaggt agagtaaggt cagcaccgcc
     1681 gtaagagcag cggcgatgta ggtgaaggcc gccgcggtca ggacccgcct ggtccctccc
     1741 gcctccgtgc cagacagtat gccgtagcgg gtcaggagtc cgtaggcgcg ggttgaggca
     1801 ttgatctcga ctggcagcgt caccacgtgg aacagtagaa ccccgaggaa cagcacgatg
     1861 gcgacgttga tcaggagcga cccgaacggg ctcgccgcgc ccccgacaaa gaaggccatg
     1921 atgaaagcca gaggccagat ctggctgctg aagttcacga tcgggaagat cccggccctc
     1981 agcttcatcg gcacgtagcc tgtggcgtcc tggatggcgt gccccacctc gtgggcggcc
     2041 acgctcaccc cggcgatgga gttgcccctg aagttgtcct cagagagcct gacgaccttg
     2101 ctcctcgggt cgtagtggtc ggtgagctgt ccggccacag gctctaccct gacgttcgtc
     2161 agagcgttgc gatcgagtat catacgggca gtctgggcgc ctgtgaggcc gctggcgctt
     2221 ctcttcttcg agtacttcga gtaagagctc ctaacccaca gggccgcagc ccccgagatg
     2281 atcattccga cgatcattat tagcaggtaa ccgccgaatc cacctggtat gaacaacgtt
     2341 aatcctccta gcagatttac tgcctgtatt ttactacata catatacgca ggaacgctcg
     2401 caaagttccg ctgagagcgc gtttgcaggg gattgggagg cgactgaccg gctgcccgtc
     2461 ggaacgacgc gtgggtagga gtcggggcgg agcctggaaa gccccgcccc ggtttggaag
     2521 actctcgccg ttcgcgctag ctctagtggc gggcctcctt gagttcgtct acgacggagg
     2581 gatcggcgag ggttgtgaca tccccaaggt cgtcctcgcc ctcggccacg ccgcggagga
     2641 cgcggcgcat gatcttgccg cttctcgtct tcggcaagtc gggggtgaag atgatctcct
     2701 cgggcctagc gtgcgggcca atggctttcc tgacctggtc gttgagctct ttgatgagct
     2761 cttcgcttcc ctctctgtcg ccttccagga tcacgtaggc tacgatagcc tggcccttat
     2821 cctcgtcgtc ccgcccgata acggcggatt cggctacggc cttgtgggcg acgagggcgc
     2881 tttcgacctc catcgtgccg atacggtgcc ccgagacgtt catcacgtcg tctattctgc
     2941 ctgtgatggt gtagtaacca tcctcatccc gctcggctcc gtcctcgacg aagtacttgt
     3001 cgccgagcct ctcccaatag gtcttacggt aacgctcggg gtccttgtag atcgtacgga
     3061 gcatgcctgg ccagggcctg gtgacgacca ggttcccctt tccggggcct tcgatcgggt
     3121 tgtcgtcatc gtcgtagatg tcgacgaaga tgcctgggaa aggccaggtg gcgctgccag
     3181 gcttggtcgg cgtgatgcct gggagaggcg cgatcatgat gccaccggtc tccgtctgcc
     3241 accacgtatc cacgatgggg caccttccgc ccccgatgtt ctcgtggtac cactcccacg
     3301 cccgcgggtt gatgggctcg cccacggtac cgagcatcct caaggacgag aggtcgtgct
     3361 tctcgatagg ctccgttccc tgcttcatga acgccctgat gacggtcggc gccgagtagt
     3421 agatggtaac cccgtgacgc tcgatcacat cccagtgccg atccgggccc gggtagctcg
     3481 gcgtgccctc gaactggacg gtggtcaccc cgttgctcaa gggtccgtag acgaggtagg
     3541 agtgccccgt aacccagcct atgtcggcct ggcaccagta gacgtcgtcc tccttgacgt
     3601 caagaaccca tttggtcgtg gccttgacct gcgtgaggta gccgcccgtg gtgtgaacga
     3661 tgcccttggg cttgccggtc gagcccgagg agtaaaggat atagaggatg tcttctgaat
     3721 ccatctcctc ggccgggcat tcggcgtctg cctcagccat caggtcgttc cagtagcggt
     3781 cgcgaccctc gaccatgtct acgtcctcgc cggtacgctg cgcgacgacc acgttctcga
     3841 tagaaggtgt gtcctggagc gcctcgtcgg cgttggcctt caagggcgtc accttgccgc
     3901 ccctcggccc accgtcggcc gtgacgagga ccttcgcctc gcagtcgttg atgcggtcac
     3961 gcaaggaatc ggcggagaaa gcgccgaaga cgaccgagtg cggggccccg atgcgggcgc
     4021 aggccagcat cgcgatcggc aactcgggga tcattggcag gtagatcgca acagggtcgc
     4081 ccttctggac cccgaggccc ttgaggacgt tggcgaacct ctcgacctcg gccttcagct
     4141 cggagtaagt gaaggtcttg ctgtcttcgg gctcatcccc ctgccagagg attgcggtct
     4201 tgtctcccct gccctgctcg acctggtagt ccagacagtt gtaagagaca ttgagctttc
     4261 cgccgacgaa ccattgggcc tcgggcgggt cccagtcgag gacccgatcc cactccctga
     4321 accagtgcag ttccctggcc cgctcggccc agaaaccctc gtagtcctgc tcggcctcct
     4381 catagaccga ggggtccttc acattcgcct ccctggcgaa ttcctctgga ggctcgtacg
     4441 tgcgtatctt tgtctcttcc gccatctcta ctcccttccc ttacgtatcc tcttatctcc
     4501 gcgcctttcc cacagcggaa ggcgcgcgaa aaacttacgg ccgtcctact gtgcaaacgg
     4561 gttccctctc caagttgacc cgtatattat tcattactgc ttttacccgc tctcgctgca
     4621 attaatcaca ggtttctcta cgctggcgcg ccacggatta ctttcgcgcc tctggcgacg
     4681 gccccactca ttccctagac tgtgccggac cttgtggagg taagagatcg ggaggcgcaa
     4741 tgggtggatt gctcgaaggc aagaaggtac tggttaccgg tgttctggac cggcgctcga
     4801 tagcctactc ggcggctcgg ctgacccagg agcagggggc tgagatcatc cttagcagct
     4861 tcggccgcgc cagaagtctc acggagcgaa cggccagacg cctccaccct gaacccccca
     4921 tcctcgaact cgatgtaaac aagccggagg acatcgaagc ggtcgcggac gacctcgcag
     4981 aacgctgggg ggatctcgac gggatcgtcc acgccatagc cttcgcccct gaggacgccc
     5041 tggggggcaa tttcctcacc gcagagtggc cctccgtcgc cacggcactc caaacctcgg
     5101 cctactcctt gaaggcgctc tccgtcggcc tcctcgatct cctcaaacca ggagcctctg
     5161 tcgtcggcct ggacttcgac gcccgtatcg cctggccagc ctacgactgg atgggtgtag
     5221 ccaaggccgc cctggagagc acttcacgtt atctcgcccg cgacctcgga gcgcacggca
     5281 tcagggtcaa cctggtctcg gcgggcccgg tcaagaccct ggccgcgaaa ggcatcagcg
     5341 gcttcg
//
LOCUS       DASTKM010000143         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_563663, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000143 DASTKM010000000
VERSION     DASTKM010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563663"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VFQ10_06020"
     CDS             complement(<1..1185)
                     /locus_tag="VFQ10_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET9964931.1"
                     /translation="MQTENPVYEEIQEILRAVELPSVALVGQRIDSPPALANIRESVR
                     EALSSVELPSGSVAIGVGSRGVARIGEVVAALVEALKDSSAEPFVVPAMGSHGASTAE
                     GQAQVLAHLGVSEEQVGCPVRATMESVRIGETSSGVAVYMDRNAYEADSVIVVNRVKP
                     HTAFRGTVESGPTKMLAIGLGKQRGAHSIHSAGWENIHRTIPEAARVAVESGKVAFGL
                     ATVENAEEEPCKIVAIPAGELEEAEALLLEEAKRNLARLPFDEIDVLVVDEIGKNISG
                     DGADPNVTGRYPTTAASGGRSVERMVFLGLTEETGGNANGLGMADVVTERLANAVDRP
                     STYMNALTSTTPAPVKTPMVMPTDEMAVAAALTMCAGVDPAEARLVRIENTLKLGRIW
                     VSE"
     gene            1917..>2162
                     /locus_tag="VFQ10_06025"
     CDS             1917..>2162
                     /locus_tag="VFQ10_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET9964932.1"
                     /translation="MRAALFIPCYVDQINPEVGVSVVRVLRKLGVEVIYPEGQTCCGQ
                     PAFNSGFFDEARTIGRRFLDVFEGERFDYIVCPSGSCT"
BASE COUNT          364 a          710 c          660 g          428 t
ORIGIN      
        1 ctccgacacc cagatcctcc cgagcttgag ggtgttctct atgcggacga gcctggcctc
       61 ggcggggtcg acgccggcgc acatggtgag ggcagcggcg accgccatct catccgttgg
      121 catgaccatg ggcgtcttga cgggcgccgg cgtggtcgaa gtgagggcgt tcatgtaggt
      181 agagggacga tccaccgcgt tggcgaggcg ctcggtaact acgtccgcca tgccgagacc
      241 attggcgtta ccgccggtct cctctgtcag gccgaggaag accatgcgct cgacggaacg
      301 gccacctgag gcagcggtcg tcgggtagcg gcctgtgacg ttggggtcgg cgccatcgcc
      361 cgagatgttc ttcccgatct catctaccac gaggacgtcg atctcgtcga acggcaagcg
      421 cgcgaggttg cgcttggctt cttcgaggag cagcgcttca gcctcctcca actccccggc
      481 ggggatcgcg actatcttgc atggttcttc ttctgcgttc tcgacggtgg cgaggccgaa
      541 ggcgaccttg ccgctctcga cggcgacgcg ggccgcctcg gggatagtcc ggtgaatgtt
      601 ctcccatccc gcagagtgta tggagtgcgc ccccctttgc ttgcccagcc cgatggcgag
      661 catcttcgtc ggaccgctct cgacggttcc cctgaacgcg gtgtggggtt tgaccctgtt
      721 gaccacgatg accgagtctg cctcgtaagc gttacggtcc atgtagacag ctacgcccga
      781 ggacgtctcc ccgattctca cactctccat cgtcgcccgc acgggacacc cgacctgctc
      841 ctcgctaacg ccgaggtgcg cgagcacctg cgcctggcct tccgcggtcg aggcgccgtg
      901 gctccccatc gcggggacta cgaaaggctc ggcgctggag tccttcaggg cctctaccag
      961 cgccgcgact acctccccta tcctggctac tcccctgctc cctacgccaa tggccacaga
     1021 gcctgatggc agctccacgg aactcagcgc ctcgcgaacg ctctccctaa tgttcgcgag
     1081 cgccggcgga gaatctatcc gctgcccgac gagcgcgacg ctggggagtt cgacggcgcg
     1141 caggatctcc tgtatctcct cgtagacggg gttctctgtc tgcactgcct cacctccgta
     1201 gggaccgcct ttacaacatt aatccggcgg gccaccctgc tcgaatcggg gtagaccgtc
     1261 gtcgacaccc tcccaggcga gtttgtcttc cagccataaa tgacgtgtgg gagggaactc
     1321 gtcggggcgg tcaaggctcg cggtagtcac gtcgatcgag tcgggctgcc cgtcatgcct
     1381 gtaggttagc gacgtgccgc aactctggca gaaggtcctg cttacttcat cggaagagcg
     1441 gtagtcggcc ggctcaccgt ggatgaggga gaaccttcta gaggggaaag taagccacgc
     1501 gaccgactgc gcgcccgacg ttctgcggca cgatacgcag tggcagaccg cagatccaag
     1561 aggctccccg gccaccctgt atctcaccct cccgcagaaa cacccaccct cgcggggacc
     1621 accgctcatc atttctccct gcccttttcg gtaccagcga acattctagc agcccgccgt
     1681 agcctattcg ccactccaaa cctgactgaa cggagcgcaa atcatggcag tggtcagcga
     1741 gatacggagc cggccggaga tttgctcgtc ggccacgatg ttcgccactg tctgctcatg
     1801 tgcgtcggag caccacccac aatcgcggtg acggttcgag ggatgggagg cgtgttgctt
     1861 ctctggatca tccggttgct atactggcct ccgaagtcgt cggggagagg aggccattga
     1921 gagcagccct tttcataccg tgttacgtgg accagatcaa tcccgaggtc ggcgtaagcg
     1981 tggtgcgcgt acttagaaag cttggggtag aggtgatcta ccccgagggt cagacctgct
     2041 gcgggcagcc tgccttcaac tccggcttct tcgacgaggc ccgcactatt gggcgccgct
     2101 tcctcgacgt tttcgaggga gagcgcttcg actacatagt ctgtccttcc ggatcgtgca
     2161 cc
//
LOCUS       DASTKM010000144         5753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_212329, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000144 DASTKM010000000
VERSION     DASTKM010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212329"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1013..2170
                     /locus_tag="VFQ10_06030"
     CDS             1013..2170
                     /locus_tag="VFQ10_06030"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET9964933.1"
                     /translation="MERSGGRRFNSATDGEILKADVADSYFHKTMRILRARGLEDTPV
                     HAELAYKTSDTDAWFVVAGLDEVAYLLDGLDVDARAVPEGTICRPHEPILTLSGPYGA
                     FAEHETAILGLLCQASGVATGAARCRLAAGERPVISFGARRMHPAVTPMIERAAYLGG
                     CDMVAVDLAVERLGIPATGTTPHALVLILGSTAEAARAFDEVMEPEVPRTIIIDTFDD
                     EKFGALLAARAIPDSISAIRMDTPGNRRGDFLDLMREVRWELDRNGFEHVKIFASGGI
                     DVEYILHLNPICDAYGVGGAIADAPMIDYSLDIVEVDGEDRSKRGKRGGRKRLLELDD
                     GRRKLLPATAPVPEGASEVLIPLQEQYFSQPDIGALRQRVLDQLQIGHFEL"
     gene            2292..2765
                     /locus_tag="VFQ10_06035"
     CDS             2292..2765
                     /locus_tag="VFQ10_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964934.1"
                     /translation="MDNSNGGARILTCPFCSETELRSFGRNSLRCDECGDVRGGALLE
                     TLYMIVTLPDAAGGHACECGHPEMRRLPDNVYRCPACGSEVTPISAPVSWKSPDHTEA
                     YWCGWLDGRYGDPEHFTNNPRLAKWESPSDRLDYYRGHRAGYEARLRKTRLVEAS"
     gene            complement(2773..3342)
                     /locus_tag="VFQ10_06040"
     CDS             complement(2773..3342)
                     /locus_tag="VFQ10_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9964935.1"
                     /translation="MVDAVSSGQLGGRVRQLRRRRGLTLEELAGLSGVSRAMISKLER
                     GEKNPTLVVAAKLAEGLGVTLSQVAGIEERREVVVVPRERRMVMRDPETGFERQLLSP
                     NFVGRGVEFIRNEIPEGSTSGAFPPHRGGVEEHIVVERGSLRATVGGEEYLLREGDAL
                     YFEADVMRRFDNVGEGECSYYLVISSRGT"
     gene            3396..4805
                     /locus_tag="VFQ10_06045"
     CDS             3396..4805
                     /locus_tag="VFQ10_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HET9964936.1"
                     /translation="MVDFLGILISALPFAVAAVMLTGLGWSGTRAGGASLATAVLGAV
                     VWPELEASAVPGALLAGLGTSAQVLYVLFGGLLLYNLLSAGGAVAAVSRFLGRLEPDR
                     VALAAGVVVGVAPFFESVTGFGVAVVISAPILLAAGFTPLRAAVLASWGQCAVPWGAL
                     GVGTVIGANLAGMGFGTLSDVSALLSIPLFPVYGVAAVAVAGGWAGVRRRGAESIVLG
                     LVAGAGTLLTSLYLVPELSGAVGGLAATAVFLARRRNRLAGVPLRALLPYAFLLLLLA
                     AANGIGALRSLFEKLGPVFDGPGLPLLVSGAFAALLLDLGGDEIGAATQRTATQWLPT
                     AGAVFTFVLAGEVVAEGGAAGLLAEGAEALGVLFPAVSPMLAALGGALTGSNAASNAL
                     FMPLQVEAARGLGVSEVLTAAVQNVAGSHASMLAPQRMVLAATATGLLGREGEILRAS
                     AAPVAVSIGMLAILGMLLT"
     gene            complement(4981..5157)
                     /locus_tag="VFQ10_06050"
     CDS             complement(4981..5157)
                     /locus_tag="VFQ10_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964937.1"
                     /translation="MQEIRDNEKIGFADGQYACSEEELYKKAISLLEKCRIETRRTSQ
                     EIPEPTAEASPKTV"
     gene            5441..5665
                     /locus_tag="VFQ10_06055"
     CDS             5441..5665
                     /locus_tag="VFQ10_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HET9964938.1"
                     /translation="MDEKRDRDVEKIYSTSEFVAKLRRLADSLEAGERFEIQVAGERV
                     YVPARAEFNVEHEREGDEEEIEFQLKWTHS"
BASE COUNT         1060 a         1801 c         1782 g         1110 t
ORIGIN      
        1 aacggatgtg caggctacat atcaaaccgt ctattggcaa ggtgaagcta tccaagctga
       61 caccggggca ggtacaaggg ttctatcagg atgggttgaa ctccggtcta gccccttcat
      121 ccgtccggta catgcacgcc acgctgcacc gtgccttgaa gcaagcacac cgctggcgtt
      181 tggtaaggga gaacgtagca gcggccacag acccaccgat accacagccc gaagaaatca
      241 gacctctgac tgcagagcag gtgaaggtgc tcttgtcaga ggcacatggc gagagactag
      301 aggctctgtt cgcagttgcg gtaacagcag gcttacgggt tggtgagctg tgttccatcc
      361 agtggtcaga ccttgacatg gagggcagga cgcttcgagt atgccggaca ctctcacgag
      421 ccaagaacgg accacgctac actacgccga agaacggcaa gggccggagt ataaagctaa
      481 ccaacatggc tacagaatcg ttgaagcgcc acagagcaag ccagaaccct gaacggttgc
      541 aagctggtgc taactggcag gacgatgacc tcatattctg tcgggaagat ggcagaccat
      601 taacaaggga tattgtggca cgctctcacc tgaagccaat cctgaagcgt gcagacttgc
      661 cagaggactg tacgccacac cagttgaggc atacttgtgc caccttgctc cttagccgtg
      721 ggatacaccc caagttcgta caggagctac ttggacacgc cacgatcgca ctgactttgg
      781 atagatatag tcactggata ccaagcatgg gcgaccagac cgcaacggca atggaagcag
      841 ccttatcgta gcggttggtc tacacgttgg tctacaaagg gtcgggcttg taacctgacc
      901 ctttacattt acctgcaaat caagactatt gacttcaagt tgacttgtag tgagtccaat
      961 tggctatgat acgccagtag gtacgctagc ggaaagggtt cgcatcttga gagtggagag
     1021 gagcggcggc aggaggttca actcggccac tgatggggag atcctgaagg ccgacgtcgc
     1081 agactcctac ttccacaaga cgatgcgcat cctgcgcgcc cgcggcctcg aggatactcc
     1141 cgtgcacgcc gagctagcct acaagacctc tgacacagac gcctggttcg tggtcgctgg
     1201 cctcgacgag gtggcctacc tcctcgacgg cctcgacgtc gatgcgcggg ccgtgcccga
     1261 gggtacgatc tgccgtcccc acgagccgat cctcaccctc tctggcccct acggtgcttt
     1321 cgccgagcac gagaccgcga tactgggcct gctctgccag gcctccggcg tggccacggg
     1381 ggcggcccgc tgcaggctcg ccgctggtga gaggcctgtg atctcctttg gcgcgcgcag
     1441 gatgcaccct gcggtcaccc ccatgatcga acgcgccgcc tacctcggcg gctgcgatat
     1501 ggtcgccgtc gaccttgccg tggaacgcct cggcatccct gccacaggca cgacgcccca
     1561 cgccctggta ctgatcctcg gctcgaccgc cgaggcggcc cgtgctttcg acgaagtgat
     1621 ggaaccggag gtgccgcgca ccattatcat agacaccttc gacgacgaga aattcggtgc
     1681 gctactggcc gccagagcaa taccggattc gatctcggcg atccgtatgg atacgccggg
     1741 caatcgccgc ggcgactttt tggaccttat gcgtgaggtc cgttgggaac tagaccgtaa
     1801 cggattcgag cacgtgaaga tcttcgcctc aggaggtatc gacgtcgagt acatcctcca
     1861 cctgaacccg atctgcgacg cctacggtgt gggcggcgct atcgctgacg ccccgatgat
     1921 cgactactcg ctcgacatag tggaggtgga cggcgaagac cgctcgaaac gcggcaagcg
     1981 tggcggacgc aagcgcctcc tggaactgga cgacggcagg cgcaagctcc tccctgcgac
     2041 cgcccccgtt ccggagggcg caagcgaggt cctgatcccc ctacaggaac agtatttctc
     2101 ccaacccgat atcggcgccc tgcgccagcg cgtccttgac cagctgcaga taggccactt
     2161 cgaactctga agcctcgtcc tcaccgccta gaccaaattg gctagtaata aatcacctat
     2221 ttgagtgatg tcgtatcgta tcttctggtg catgattatg gtgtgctcga agcgacaaga
     2281 agggaggcgt catggacaac tcgaacggcg gagccagaat cctgacctgc cccttctgct
     2341 cggagaccga actgcgatcc ttcggccgca actccctccg ctgtgacgag tgtggagacg
     2401 ttcgcggcgg cgcgctgctg gagacgctct acatgatagt cacgcttccc gacgcggccg
     2461 gcggccacgc ctgcgagtgc ggtcaccccg agatgagacg cctgcccgac aacgtgtacc
     2521 ggtgtccagc ctgcggatct gaggtcaccc ccatctccgc cccggtgtcc tggaagtccc
     2581 cggatcacac cgaggcgtac tggtgcggct ggctcgacgg ccgttacgga gatccggaac
     2641 acttcaccaa caacccccgt ctggccaaat gggaaagccc ttcagaccgc ctcgactact
     2701 accgcggcca ccgcgccggc tatgaagccc gtctgcggaa gaccaggctc gtcgaagcct
     2761 catagcactt cgtcacgttc cccgcgagct gatcaccagg tagtagctac actccccctc
     2821 accgacattg tcgaagcggc gcatcacgtc agcctcgaag tacagcgcat cgccttcgcg
     2881 tagcagatac tcttcgccgc cgactgtcgc cctcaaactc ccccgctcga cgacgatgtg
     2941 ctcctcgacg cccccgcggt gcggcggaaa cgcgcccgaa gtggagccct cggggatctc
     3001 attgcggatg aactccacac ccctcccaac gaagttcggc gaaaggagct gacgctcgaa
     3061 gcccgtctcg ggatcgcgca tcaccatgcg cctctccctg ggcacgacga caacctcgcg
     3121 ccgctcctct attcccgcaa cctgcgagag cgttactcca aggccctcag ccagcttcgc
     3181 cgcgacaacg agcgtggggt ttttctcccc gcgctcgagc ttcgagatca tggcccgact
     3241 caccccagag agcccggcaa gctcctccag cgtcagacct cgcctccggc gtaactgcct
     3301 tacacggccc cccaactgcc cggaagagac cgcatcaacc accgtagaac tcccgcttgc
     3361 agcttcatca tccatttggt agaagttatt ctatcatggt agattttctg gggatattga
     3421 tctctgcgct gccttttgcg gtggccgctg tgatgttgac gggtctggga tggtctggga
     3481 ccagggctgg tggggcgtcg ctggcgacgg cggtgttggg ggcggtggtg tggccggagc
     3541 ttgaggctag cgcggtgcct ggggctttgc tggctggtct ggggacctcg gcccaggttc
     3601 tgtacgtgct cttcgggggg ttattgctct acaacctgct ctcggcagga ggggctgttg
     3661 ctgctgtgtc gcggtttctc gggagattgg agcccgaccg tgtcgcgctc gctgccggag
     3721 ttgtggtcgg ggtggcgcca ttcttcgagt cggttacggg attcggggtg gccgtggtca
     3781 taagcgcccc gatcctgctc gccgccgggt tcacaccctt gagggccgcg gtgctggcga
     3841 gctggggcca gtgcgccgtg ccctggggag cgctcggtgt agggacggtg atcggggcca
     3901 accttgcggg gatgggcttc gggacactct cagatgtgag cgcattgttg agcatcccgc
     3961 tcttccccgt ctacggtgtc gcagccgtcg cggtcgcggg cgggtgggcg ggcgtacgcc
     4021 ggcgcggcgc ggagtcgata gtattggggc tggtcgcagg ggcgggaacg ctcctgacga
     4081 gcctgtacct ggtccctgag ctctccggcg ccgtcggagg gctcgctgct acggcggtct
     4141 tcctggcgag gaggcgtaac cggctcgccg gagttccttt gcgcgcgctg ctcccctacg
     4201 cgtttctact attgctgcta gcggcggcga acggcatcgg ggcgttgcgc tctctcttcg
     4261 agaagctagg tccagtcttc gacggtccag gccttccttt gctcgtttcc ggagcgttcg
     4321 cggcgctgct gctggatctc ggcggggacg aaatcggggc tgcgacgcag agaactgcga
     4381 cgcagtggct tcccacggca ggggccgtct tcacgttcgt tctggcggga gaggtggtgg
     4441 cagagggtgg cgcagccggg ctgcttgccg aaggcgcaga agcccttggg gtcctatttc
     4501 ctgcggtctc gcccatgctc gcggcacttg gcggtgcact caccggctcc aacgcggcct
     4561 cgaacgcatt gttcatgccg ctccaggtcg aggctgctcg gggactcggc gtatctgagg
     4621 tactaacggc tgccgtgcag aacgtcgccg ggagccacgc cagcatgctc gccccgcagc
     4681 gcatggtcct cgcggccacg gccacgggcc tactcggcag ggaaggcgaa atcctgcgcg
     4741 cttcggccgc cccggtggcc gtctccatcg gcatgctcgc gatcctcggg atgctcctta
     4801 cttagagcga gtgtcggtgg gatcgtgggg tgtatgcggg caccgcgcat accatgcgct
     4861 cagaatccta gaggactgac gctcggcgcc acgaccacac cgctccggca ctctcgccga
     4921 cgcgactcct tcggacactc tgcagccggt tagactaacc tccctggccc cgctcgggcg
     4981 ttagacggtt tttggtgagg cctcagcggt tggctccggt atctcctggc tcgtccgccg
     5041 ggtttcgatc ctgcacttct cgagaagtga gatggccttc ttgtagagct cttcttcaga
     5101 acaggcgtac tggccgtccg cgaagcctat cttctcgttg tcacggattt cctgcatcac
     5161 cttgaggcta cacgttgcac aacgcctttg ggttacagaa ttgcaacatt ccggtaagag
     5221 cgcggtaacg caaccgaggg ttcgacccga cctcgttgtc cacatcaggg ttgaggagat
     5281 cagaccgagt cgcggacact gcagggtcca gaataagata gcaggttggc ggaactacgt
     5341 ttgcaggact cctgaaaagt cccgctctga cttggctgtg gccctcggac cctgtaaggt
     5401 accgagaggt cgagcgttcg gcgaatccgg aagaggagcg atggacgaaa agcgagacag
     5461 agacgtcgag aagatctact ccacctcgga gttcgtggcg aagctgcgaa ggcttgccga
     5521 ttcgctggag gcgggcgagc ggttcgagat ccaggttgcc ggggagagag tctacgtgcc
     5581 cgcccgtgcc gagttcaacg tcgagcatga gcgggagggc gatgaggagg agatcgagtt
     5641 ccaactcaag tggacccact cctgagtacc tactccatac caaccgccgt accctcgagg
     5701 gctcgaccga ggagcacccc ctcgcctcac ggagccacat tcgcggcgca gtg
//
LOCUS       DASTKM010000145        12609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_212332, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000145 DASTKM010000000
VERSION     DASTKM010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12609
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212332"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VFQ10_06060"
     CDS             <1..757
                     /locus_tag="VFQ10_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9964939.1"
                     /translation="EHLRLWIERSVWSRDGDPDEHLNALMRLATEYFLTAELSKSGKR
                     LVGDKSPLLDEEFIREVGAVYPGSRVIHIIRDGRDRTVSSMHRGLRRASQGYLHRLSP
                     EELARGKALRAGRRERTGAFTEEWLRQAARHWRLLVGRAAEDGPALLGPDYTEVRYED
                     LLERPNEEVQRLLGFLGVDTDERLVEHCVSSASFEKLSRGRQRGQEDTSSFYRKGVAG
                     DWKNHFTEEDRRVFKEEAGELLIRLGYEDDLDW"
     gene            767..2224
                     /locus_tag="VFQ10_06065"
     CDS             767..2224
                     /locus_tag="VFQ10_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET9964940.1"
                     /translation="MTARIMSRVGRILLLSGACLALGFFLPGELERESIEAVGRPNII
                     FVITDDLDAASVSKVPSLTKYMAGKGMTFDNAFVTSALCCPSRATILTGQYPHNHLVR
                     SNVPPVGGFKTFRDLGRERSTLATWLDDAGYETAMFGKYLNGYGKIGRNHVPAGWDEW
                     YGAVEDARLNQNGQLVTYQADTYLDDALSGLAQDFIRRQERKDAPFFVYLSVHAPHAP
                     AKPALRHEGLYEGLRAPRTPSFDEADVSDKPRWVRNLSLDSAEEKRIDELYSKRLKTM
                     AAVGEMIGGLLRTLQQSGKLDNTYVVLTSDNGYHMGQHRLGLGKQSAYEEDIRVPLMI
                     RGPGVPAGVSRDEMVLNNDFVPTFADLAGLPPPASVDGRSFASLLDKRQGNDPASWRT
                     AFEIRNWDNKKDQASYRAVTPVPPYRAVRTKRYLYVEYEAGERELYDLRRDPYELNSL
                     HDSADPDLVSKLDARLDALRDCAGKGCRTAEDGSP"
     gene            complement(2221..3054)
                     /gene="rfbD"
                     /locus_tag="VFQ10_06070"
     CDS             complement(2221..3054)
                     /gene="rfbD"
                     /locus_tag="VFQ10_06070"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET9964941.1"
                     /translation="MPKVLLSGSGGQLGLCLAELLPLRGHEVVALSKGELDVVDFGGV
                     RLALEDHAPDVVVNAAAYTNVDGCETEAELAYRVNALGPRNLAQICEDLGCDLLHVST
                     NYVFDGESTRPYEPFDTPNPISVYGRTKLAGEEYVRQLTSRWYVVRSAGVYGRGHNFV
                     RTMLRQGAERDVLKVKDDEFISPTYAADLAEGIVGVIEDRHYGLYHLTNSGSCTWYEF
                     AKEIFSLAGVDVEVVPVPGSEYPLPAARPANGVLSALGSPELRHWREALEDYLRREKV
                     T"
     gene            complement(3065..4405)
                     /locus_tag="VFQ10_06075"
     CDS             complement(3065..4405)
                     /locus_tag="VFQ10_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9964942.1"
                     /translation="MRILFAAHQFFPEHYAGVETVTLSLAQEFKARGHEPYVLAPKRS
                     IPVNDIEPGEVEDYEFGGIPVRRVGRPKEGISRPYSLDYENEVMAGRTREYMRKVEPD
                     IVHAEHFQGLSASVIPAIKEFGVPLVYTATDFWTFCPVVDLRRHDGVMCRGPEISHCT
                     RCIASRYPGTRMKQAVDLTPPGAIKVAGALSGTRLSGLSQSLRQVRALKERPERIREK
                     MRLVDHVIVYTHLMRELLLANGIGAGKIEVSHYGIDTSGIRREDRPFSLPLRVGFVGT
                     LAPHKGCDVLIRAFRDLPPELAATLDIYGNLERFKPFVEELRALAGGDGRIDFAGPFA
                     REEVGDVLSRMDVLVVPSRWYENGPGVIFEAFAAGIPVVATNLGGMSEFVRHERDGLL
                     FGLEDPGDLARQLRRLIEEPGLLERLRTGIEPVKTVQENVDELETLYGGLLARV"
     gene            complement(4409..5539)
                     /locus_tag="VFQ10_06080"
     CDS             complement(4409..5539)
                     /locus_tag="VFQ10_06080"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9964943.1"
                     /translation="MNLERVVERLRDYRLKNERFDRLVGMVLSNEKAMRVARATVAAS
                     RSVSRRGQTVAAGDYDTVFFVAGEMRSGTSWLRHSLSDHPEIACGQEGSFFGRGYDRE
                     EIPVYTGPVSSLTRALALSRDLKTWHALPWNLWSDGYDEDLRNLSRLSVDYFLSKEVA
                     RTGRRIVGDKSPQHTENLDEIHEIYPDARIIHIVRDGRDVAVSAMHHWWRLAKDREGG
                     VFDLAPEELRIRDDYAEDRERFLSEGRSIFTQERLAQLARRWEYRTGKGSHDGRTLYG
                     ERYLEVRYEDLVVDTPSVFGEILEFLEAGSEDRVIERCLRSSSFERTSSRRQGEEDSA
                     SFFRKGVVGDWREVFTERDRELYKDLAGERLVEFGYETSYDW"
     gene            complement(5661..6632)
                     /locus_tag="VFQ10_06085"
     CDS             complement(5661..6632)
                     /locus_tag="VFQ10_06085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9964944.1"
                     /translation="MAETVSVVIPTFNAGPGFEELLRKLDAQEGDFEREVVVVDSGST
                     DGTAELAARHGAILHSVSKASFDHGATRDLGVSLSSGEYVALTVQDAMPLDERWLATM
                     VENLQNDGRIAAVYGRHIPRPDAGVMTRALVGNLAVADTERREQEIRDTTQDLSMPPA
                     HKRRIAAFDNVSSCLRRQVWEEFPFGEADFAEDLRWGKKVVEAGYTIVYEPCSVVVHS
                     HERGVLYDLRRHYVDQLVLDELFGARLVPNLPRLILGIPYATRHLYSLLRREEPAPEK
                     RPKLLFDAATYALASQLGAYLGCKVPSLSRLSPKLHARLHRILGKGV"
     gene            complement(6635..7582)
                     /locus_tag="VFQ10_06090"
     CDS             complement(6635..7582)
                     /locus_tag="VFQ10_06090"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9964945.1"
                     /translation="MLRRGRRWLSGKYREVPLLAASLRRRFEGASANGVKPENIVWIF
                     GSGRSGSTWLRSMMGDPSRHLVWEEPMVGQLFGNFHRRAEKANLVRPDFVMADATRRG
                     WIHSIRNFVLDGARYARPRLGPDHYLVVKEPNGSVGAPLLMEALPESRMILLIRDPRD
                     VVSSTLDGARKGSWLYERKDRGGWKRDSLPDKNPDAFTRRWANVYMQHAGNAKKAYDA
                     HKGPKVMVRYEDLRADTLGTMRRIYSTLGIPVDEHELARAVEKHSWENIPEKKKGGGK
                     FYRKATPGGWQEDLSPRQIKQVERITAPLLEEYYSGDVP"
     gene            complement(7627..8721)
                     /locus_tag="VFQ10_06095"
     CDS             complement(7627..8721)
                     /locus_tag="VFQ10_06095"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9964946.1"
                     /translation="MSVSRYRGALRYLREGPARLGSTVDRLASRLRGQSPNGRAFRGE
                     EMPVFFVVGLAKSGTTWLMKTLDAHPEVLCKGEGRFFGEEYRREVLAQTQTKQQPSSL
                     YNALLGSEYLRLWIERSVWSRDGDPDEHVRDLVRLATDYFLTEELSKSGKKVVGDKTP
                     LLGPKFVEEIYEIYPESRVIHIIRDGRDQAVSFIHQQGNRAKRGRTHRLSAEELARSE
                     AYRRSPRELAQRGEGLFAEKTLRKAAQNWAIRVGQAIEDGPALFGDGYTEVRYEDLLE
                     RPNEEVQRLLGFLGVDTDETLVEHCVSSASFEKLSRGRERGQEDPSSFYRKGVAGDWK
                     YLFDERDRQVYKEEAGELLIRLGYEKNDGW"
     gene            complement(8785..9765)
                     /locus_tag="VFQ10_06100"
     CDS             complement(8785..9765)
                     /locus_tag="VFQ10_06100"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9964947.1"
                     /translation="MPVFRYPKVSGVARRVRAVLGLEAGVTRAALPDPTGTSAEGPSV
                     GGIAPENMIWIFGTGRTGSTWLAAMMEEPEGHDVWFEPRVGTVFDPQRFQRHGGKHFI
                     LASQYKDVWLRSIRNFILDGANARFPELDGGYLAVKEPGGSVGAGLIMEALPESGMVL
                     LIRDPRDVVASWLDATRKGGWQTRRRGEGGRRAESLAETKPNAFVRRHANAYLQHVGS
                     ARRAYEAHEGRKVVVRYEDLRADTLGTMKRMYGELNVPVEEARLAKAVEKHSWENIPE
                     EEKGQGKFYRKATPEAWREDLTRRQVKTVERITAPLLEEFYPTGPAEQQG"
     gene            complement(9776..10633)
                     /locus_tag="VFQ10_06105"
     CDS             complement(9776..10633)
                     /locus_tag="VFQ10_06105"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9964948.1"
                     /translation="MRPENLVWVFGTARTGSSWLSAIMGEIGGYSRWHEPLVGHLFGN
                     LYYERAGHRSEDEHFILGARYREIWLGTLRRFVLDSAAARFPGVAAKDGRYLIIKEPQ
                     GSMGAPLLMEALPESRMILLVRDPRDVVASNLDAHKKGTWTADLMRKGGKEKPPSLAE
                     SRPDAFVKGQARRYVRDVGNAKLAYDSHRGLKVLVRYEDLRADTLGTMKRIYTALEIP
                     VEEAELARSVEKNAWENIPEQEKGEGRFHRKAKPGGWREDLTPEQAEIVEVVTGSLLR
                     ELYPVRSNA"
     gene            complement(10699..11640)
                     /locus_tag="VFQ10_06110"
     CDS             complement(10699..11640)
                     /locus_tag="VFQ10_06110"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9964949.1"
                     /translation="MTPRFKRAWRRALRKGASLRSRARRSPGGVRPENIVWIFGTGRS
                     GSTWLGYMMEGLDGCAMWNEPLVGNLFGNFHYFRVGGRKPGRQYILGEPYKETWLGPM
                     RDLVLGGAAARFPELMEGGYLIIKEPNGSIGSPLLMEALPESRMIFLIRDPRDVVASS
                     MDARSEGSWLQERREARRRTSKPDRNPNVYVRMRANSYVQQIEKTGQAYRAHGGHKAM
                     VRYEDLRADTLETMKRIYTALEIPVEEAELARSVEKNSWENIPEQEKGEGKKRRKAKP
                     GGWKEDLTARQVEIVEEITGPLLNEFYPHTATTRGAL"
     gene            11899..>12609
                     /locus_tag="VFQ10_06115"
     CDS             11899..>12609
                     /locus_tag="VFQ10_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9964950.1"
                     /translation="MRVKTMVVGEGRLQGYTFAAALLWAVLLLILAPGSKGEPAAQPG
                     VDNDPEGAPYVAGELLVAYEPGTSEEAEETVVRRSGARTLENLPGEVRLVSFPAIRRE
                     ASEAVRERALARALAYLGHKAAVEAVDYNYLREPYFIPNDPMLANQWGLISARFGGAW
                     NNAKGGRAKVAIVDSGAYSNHPDIGRIIAQRDFVEDDAVADDDNGHGTHVTGIAGALT
                     DNGKGVAGGCFDCELLIAK"
BASE COUNT         2181 a         4145 c         3827 g         2456 t
ORIGIN      
        1 agagcacttg aggctctgga tcgaacgctc tgtgtggagc cgggatggag atcccgacga
       61 gcacctgaac gccctgatgc gtctggccac agagtacttc ctaaccgccg aactctcgaa
      121 gtccggcaag aggctcgtcg gagacaagtc gcccctcctg gacgaagagt tcatacggga
      181 ggtcggagcc gtgtatccag ggtccagggt catccacatc ataagggacg gcagggatag
      241 gacggtctcc tccatgcacc gcgggttgcg ccgcgccagc cagggctacc tccacaggct
      301 ctcgcccgag gaactggcaa ggggcaaggc tctgcgcgca gggcggcggg agcggacggg
      361 cgcgtttaca gaggaatggc tgcggcaggc agccaggcac tggagactcc ttgtgggcag
      421 ggccgccgaa gacggcccgg ctttgctcgg gccggattac accgaggtgc gctacgaaga
      481 cctgctggag cgccccaacg aggaagtcca gagattgctc ggcttcctcg gggtcgatac
      541 ggacgagagg ctcgtcgagc actgcgtgag ttcggcgagc ttcgagaagc tctccagggg
      601 gcgccagcgg gggcaggagg atacgtcctc cttctatcgt aaaggcgttg cgggtgactg
      661 gaagaatcac ttcaccgagg aggacaggcg cgtcttcaaa gaggaggccg gggagctctt
      721 gatcaggctc ggttacgagg atgacctcga ctggtagaac cacgtcgtga ctgcccgcat
      781 catgagcagg gtaggcagga tcttgctgtt gtccggggca tgcctggcgc tcgggttctt
      841 cctgcctgga gagttagagc gggagtccat agaagcagtg ggccgtccca acatcatctt
      901 cgtaattacg gatgatcttg acgctgcgtc ggtctcgaag gtaccgtccc tcacgaagta
      961 catggcaggt aagggaatga ccttcgacaa cgcattcgtc acctccgcgc tctgctgtcc
     1021 ctccagggca acgatcctga ccggccagta cccgcacaac catctggtca ggagcaacgt
     1081 tcccccagta ggaggattca agacgttccg cgacctcggg cgagagagat ccacgctggc
     1141 cacctggctc gacgacgcag gttacgagac ggccatgttc ggcaagtatc tcaacggcta
     1201 cggcaagatt ggccgcaacc acgttccggc ggggtgggac gaatggtacg gggccgtaga
     1261 ggacgcgcgg ctgaaccaga acgggcagct ggtcacgtac caggcggaca cctacctcga
     1321 tgatgcgctt tcgggtctcg cccaggactt cataagacgc caggaacgca aggatgctcc
     1381 atttttcgtg tatctctccg ttcacgcccc ccacgcgccc gccaaacccg ccctgagaca
     1441 tgagggtctg tacgagggcc tgcgggcgcc ccgcacgccc tccttcgacg aagcagacgt
     1501 ctccgacaag ccacggtggg tgcgcaacct atcgctagac tccgcagagg agaaacgtat
     1561 cgacgagctc tacagcaaga ggctaaagac gatggccgcg gtaggcgaga tgatcggcgg
     1621 gttgctaagg acgctccaac agagcgggaa gctagacaat acctatgttg tcctcaccag
     1681 cgacaacggc tatcacatgg gccagcatcg actcggactc ggcaagcaga gtgcctacga
     1741 agaggacata agggtgccat tgatgatccg agggccaggc gtgcctgcgg gggtcagtag
     1801 ggacgagatg gtcctgaaca acgacttcgt cccgaccttc gccgacctgg cgggcttgcc
     1861 gcctccggcc tccgtagacg gccgctcgtt cgcgtccctg ctcgacaaac ggcagggtaa
     1921 cgacccggcc tcgtggcgca cggctttcga gatccgcaac tgggataaca agaaggacca
     1981 agcgtcttat cgggccgtaa cgcctgtgcc tccttaccgg gccgtaagga ccaagagata
     2041 cctttacgtc gagtacgagg ccggggagcg cgagctgtac gacctcagga gagaccccta
     2101 cgagctgaac agcttgcatg actcggcgga cccggacctc gtctcgaaac tcgacgcgcg
     2161 cctcgacgcg ctgcgcgact gcgccgggaa aggttgccgc accgcagagg acgggtcgcc
     2221 ctaagtaacc ttctcgcgcc ggagatagtc ctccagggcc tcgcgccagt ggcgcaattc
     2281 cgggctcccc agggcggaga ggacgccgtt ggcgggccgc gccgcaggga gagggtactc
     2341 cgagccaggg acggggacga cctcaacgtc gacgccggcg agtgagaaga tctccttcgc
     2401 gaactcgtac caggtgcagg agcccgagtt ggtaagatgg tagaggccat agtgtcggtc
     2461 ttcgatcacc cccacgatcc cttccgcgag gtccgcggcg taggtcggcg agatgaactc
     2521 gtcgtccttg accttcagca cgtccctctc cgctccctgg cgcagcatgg tccgcacgaa
     2581 gttgtgcccc cttccgtaga cgcccgcgga tcggacgaca taccagcggc tagtgagctg
     2641 cctgacgtac tcctctccgg caagtttggt gcggccgtag acgctgatcg ggttcggcgt
     2701 atcgaagggc tcgtagggtc gtgttgactc gccgtcgaag acgtagttcg tgctgacatg
     2761 caagagatcg cagcctagat cctcacagat ctgggccagg ttcctcggac cgagcgcgtt
     2821 gaccctgtaa gcaagctcgg cctctgtctc gcagccgtcg acgttggtgt aggcggccgc
     2881 gttcacgacg acatctggag catggtcctc cagcgccagc cgcacgccac cgaagtccac
     2941 cacgtcaagc tctcccttgg agagtgcgac gacctcatgg ccgcgcaggg gaaggagttc
     3001 tgcgagacag aggccgagct ggccgcccga gccgcttagc agcaccttcg gcacgttgtc
     3061 ctccctagac tctcgccagc agtccaccgt acagagtctc gagctcgtcc acgttctcct
     3121 ggacggtctt gaccggttcg atcccggtcc ggagcctctc gagcaggcct ggttcctcga
     3181 tcaggcgcct gagctggcgc gccaggtcgc cagggtcctc cagtccgaag agcaacccgt
     3241 ctctctcgtg tctcacgaac tctgacatcc cgccgaggtt cgttgccacc accgggatac
     3301 cggctgcgaa tgcttcgaag atcacaccag gaccgttctc gtaccagcgg gatggcacga
     3361 ccaggacgtc catcctggag aggacgtcac ctacctcttc acgcgcaaag gggccggcga
     3421 aatctatccg cccgtctccc ccggccaggg cgcgcagctc ttctacaaag ggtttgaacc
     3481 tctccaggtt accgtagatg tcgagggtcg cggccaattc gggcgggaga tctctgaagg
     3541 cccggatcag gacgtcacag cccttgtgcg gcgccagggt tcctacaaag ccgacccgga
     3601 gcggcaggga gaagggtctg tcttccctac gtatgcccga ggtgtctatg ccgtagtggg
     3661 agacctcgat cttgccagct cctatgccgt tggcgagcaa caactcccgc atgaggtggg
     3721 tgtagacgat aacgtggtcc accagcctca tcttctccct gatgcgctcc gggcgctcct
     3781 tgagggccct cacctggcgc agcgattggg agaggcccga gagcctggtt ccggagagag
     3841 cgccagcgac ctttatggcg cccggcggcg tcaggtcgac cgcctgcttc atcctggtgc
     3901 caggatatct gctcgctatg cagcgggtgc agtgagagat ctcgggcccc ctgcacataa
     3961 ccccatcgtg gcgcctgagg tcgacgaccg ggcagaacgt ccagaagtct gtcgccgtgt
     4021 acacgagcgg cacgccgaac tccttgatag cggggatcac gctcgccgag agtccctgga
     4081 agtgctcggc gtggacgatg tcgggctcta ccttgcgcat gtactcgcgg gtcctacccg
     4141 ccatgacctc gttctcgtaa tccaggctgt agggccgtga gataccctcc ttgggacggc
     4201 ccaccctgcg cactggtatg cccccgaact cgtagtcttc gacttcccct ggctcgatgt
     4261 cgttgacagg gatgctccgc ttgggggcga gcacgtaggg ctcgtggccc ctcgccttga
     4321 actcctgcgc cagggagagc gtgacggtct cgacaccggc gtagtgctcg gggaagaact
     4381 ggtgtgcggc gaagagtatc ctcacgccct accagtcata tgacgtctcg tacccgaact
     4441 ccaccaatcg ctccccggcc agatctttgt acagctctcg atctcgttcc gtgaagactt
     4501 ccctccagtc gccgaccaca cccttcctga agaaagaggc ggaatcctcc tcgccctgcc
     4561 tgcggctgga agtccgctcg aagctgctcg accgcaggca gcgctctatg acgcgatcct
     4621 cgctgcctgc ctcgaggaac tccagtatct ccccgaagac agagggcgtg tcgactacta
     4681 ggtcttcgta cctgacctcc aggtatctct cgccgtagag ggttctccca tcgtggctgc
     4741 ctttccccgt cctgtactcc caccggcggg caagctgggc cagccgctcc tgcgtgaaga
     4801 tcgaacgccc ctcggacagg aaccgctccc tgtcttcggc gtagtcgtcg cggatgcgga
     4861 gctcttccgg cgcgaggtcg aagacgcccc cttccctgtc tttcgccagc cgccaccagt
     4921 gatgcatcgc cgagaccgcc acgtccctac cgtccctgac gatgtggatt atcctggcgt
     4981 cggggtagat ctcgtgtatc tcgtccaggt tctcggtgtg ctgggggctc ttgtccccca
     5041 cgatcctgcg ccccgtgcgg gccacctctt tggagaggaa gtagtcgacc gagagccgag
     5101 agagattcct caagtcttcg tcgtagccgt ctgaccacag gttccacggc agcgcgtgcc
     5161 aggtcttcaa gtccctggag agagcaaggg cgcgagttag gctcgagacg ggcccggtgt
     5221 agacgggtat ctcctccctg tcgtagccac gtccgaagaa gctcccctcc tggccgcagg
     5281 cgatctcagg gtggtcgctc aggctgtgcc gcaaccagga ggttcccgag cgcatctctc
     5341 ctgccacgaa gaacaccgtg tcgtagtcac ccgcagctac ggtctgcccc ctgcgggaga
     5401 cggaacgaga cgctgcgact gtggcgcgcg cgaccctcat ggccttctca ttggagagca
     5461 ccatccccac cagccgatcg aaccgctcgt tcttcaaccg gtagtcgcgc aggcgctcga
     5521 cgacgcgttc gaggttcatg gcggagatta tagtttcagc atgtcagcat gtcagcacgc
     5581 tcaggccctt aggccctcgc ttgtcagcag ggtcctgccc atgagaaagc tgactagcga
     5641 agcccggaag gagatccgca tcacacccct ttgccgagga tgcggtggag cctggcgtgg
     5701 agtttcgggc tcaggcggga gagggaaggg accttgcacc ccaggtaggc gccgagctgg
     5761 gaggcgaggg cgtaagtcgc cgcgtcgaac aggagctttg gcctcttttc aggtgccggt
     5821 tcctcgcgcc tgaggaggct gtagaggtga cgggtcgcgt agggtatgcc gaggatcaga
     5881 cgtggcaggt tcggcaccag gcgggccccg aagagctcgt cgaggacgag ctggtctacg
     5941 tagtggcgcc tgaggtcgta gaggacgccg cgctcgtggg agtggacgac gaccgagcac
     6001 ggctcgtaga cgatagtgta ccccgcctcc actaccttct tgccccaacg caggtcctcg
     6061 gcgaagtctg cctcgccgaa tgggaactcc tcccagactt ggcggcgcag gcaggagctc
     6121 acgttgtcga aggcggctat gcggcgcttg tgggccggcg gcattgacag gtcctgtgta
     6181 gtgtcccgaa tctcttgctc ccgcctttcg gtatctgcga cggcaaggtt gccgaccagc
     6241 gccctggtca tgacgccagc gtcgggcctg gggatgtggc ggccgtagac cgcggcgatc
     6301 cggccgtcat tttgcaggtt ctcgaccatg gtggccagcc acctttcgtc gagtggcatg
     6361 gcgtcttgaa ccgtaagggc cacgtactct ccggatgaga gcgagacccc gagatcccgc
     6421 gtcgcgccgt ggtcgaaact ggctttcgaa acgctgtgca aaatcgcgcc gtgccttgcg
     6481 gcgagctccg cggtgccgtc ggtcgagccc gagtcgacga cgacgacctc ccgctcgaag
     6541 tctccctcct gcgcgtcgag cttccgcagc agctcctcga aaccggggcc ggcgttgaag
     6601 gtgggtatga cgaccgagac cgtttcagcc aatattatgg cacatcgccc gagtaatact
     6661 cttcgagcag gggagccgtt atcctctcga cctgctttat ctgccttggg gagaggtcct
     6721 cctgccagcc tcccggcgtc gccttgcggt agaacttgcc cccgcccttt ttcttctccg
     6781 ggatgttctc ccaggagtgc ttctccacag cccgtgccaa ctcgtgttcg tccacaggga
     6841 taccgagcgt cgagtagatg cgccgcatgg ttcccagagt gtctgccctg aggtcctcgt
     6901 agcggaccat gactttcgga cccttgtggg cgtcataggc cttctttgcg ttccccgcgt
     6961 gttgcatgta gacgttcgcc cagcgcctgg tgaacgcgtc ggggttcttg tccggcagcg
     7021 aatcgcgctt ccatccgcct ctgtccttgc gctcgtagag ccagcttccc tttctggctc
     7081 cgtcgagcgt cgatgagacc acgtctctgg ggtcccgaat caagagtatc atgcggctct
     7141 cgggcaaggc ttccatgagc agaggagccc cgaccgagcc gttcggctcc ttgaccacga
     7201 ggtaatggtc ggggcccagg cggggtcttg cgtagcgtgc gccgtccagc acgaagttcc
     7261 ttatggagtg gatccaaccc ctacgggtcg cgtcagccat gacgaagtcg ggtcggacga
     7321 ggttcgcctt ctctgccctg cggtggaagt tgccgaagag ctggccaacc atgggctcct
     7381 cccataccag atgccggctg ggatctccca tcatgctcct gagccacgtg ctgccgctgc
     7441 gtcccgagcc gaagatccag acgatgttct caggctttac accgttagca gaggcgcctt
     7501 cgaacctcct tcgcagcgag gctgccagca gggggacctc cctgtacttg ccggacagcc
     7561 agcggcggcc cctgcgcagc acggcgggca agccctcttt cgccctcctg atcctggtct
     7621 ccaatgctac cagccgtcgt ttttctcgta gccgagcctg atcaagagct ccccggcctc
     7681 ctctttgtag acctgcctgt cgcgctcgtc gaacagatat ttccagtcac ccgcaacgcc
     7741 tttacgatag aaggaggatg ggtcctcctg tccccgctca cgtcccctgg agagcttctc
     7801 gaagctcgcc gaactcacgc agtgctcgac aagcgtctcg tccgtgtcga ccccgaggaa
     7861 gccgagcaat ctctggactt cctcgttggg gcgctccagc aggtcttcgt agcgcacttc
     7921 cgtatagccg tctccgaaca aggcgggtcc gtcctcgatg gcctggccca ccctgatggc
     7981 ccagttctgg gcggctttcc tcagcgtctt ctcggcgaac agcccctcgc ctctctgggc
     8041 gagttccctg gggctcctgc ggtaagcctc gctcctcgcc aactcctcgg ccgagagcct
     8101 gtgcgtacgg ccccgcttgg cgcggttgcc ttgctggtgt atgaaggaga ccgcctggtc
     8161 cctgccgtcc cttatgatgt ggatcaccct cgattccggg tagatctcgt atatctcctc
     8221 cacgaacttc gggcccagaa ggggtgtctt gtctccaacc accttcttgc cgctcttgga
     8281 gagctcttct gtgaggaagt agtccgtggc cagcctgacg agatccctga catgctcgtc
     8341 gggatctcca tcccggctcc acacagagcg ttcgatccag agcctcaggt actcggagcc
     8401 gagcagcgcg ttgtacagcg agctgggctg ctgcttggtc tgcgtctggg ccaggacttc
     8461 ccgcctgtac tcctcgccga agaacctacc ctcgcccttg cagagtactt cgggatgggc
     8521 gtctagggtc ttcatcaacc acgtggtgcc tgatttcgcc agcccgacga cgaagaacac
     8581 cggcatctct tcgccgcgga aggccctccc gttcggcgat tgccctctaa ggcgggacgc
     8641 gagcctgtcg acggtcgatc cgaggcgcgc aggcccctcg cgtaggtacc tgagcgcgcc
     8701 gcggtaccta gagaccgaca cagccatact ccgcacgggc tcgccccacc agagccgctt
     8761 ggcgcgcact gtcttgggaa gccctcatcc ttgctgctcc gcaggccctg tggggtagaa
     8821 ctcttcgaga agcggcgccg ttatccgctc gacggtcttc acctggcggc gcgtaaggtc
     8881 ctcgcgccac gcctcaggag tggccttgcg gtagaacttg ccctgcccct tctcctcttc
     8941 cgggatgttc tcccaggagt gcttctccac agctttggcg agccgtgcct cctctacagg
     9001 gacgttcaac tcgccgtaca tacgtttcat cgtccccagg gtgtctgccc tcagatcctc
     9061 gtagcggacc accaccttac ggccctcatg cgcctcatag gcccgcctgg cgctcccgac
     9121 gtgctgcagg taggcgttgg cgtggcgcct cacgaaagcg ttcggtttcg tctcggcgag
     9181 cgactccgcg cgacggcccc cttcgcccct acgcctggtc tgccaaccac ccttcctggt
     9241 ggcgtcgagc caggaggcga caacgtccct cgggtcacgg atcaggagca ccatcccact
     9301 ctccggcagg gcctccatga tcaggccggc ccccaccgag ccgccgggct ccttgaccgc
     9361 caggtatcca ccatccaact cagggaaacg cgcgttggcc ccgtccagga tgaagttcct
     9421 gatggaacgg agccagacgt ctttgtactg ggacgcgagg atgaagtgct ttccaccgtg
     9481 gcgctggaac ctctgggggt cgaagacggt tccaaccctg ggctcgaacc acacgtcatg
     9541 accctccggc tcctccatca tcgcggcgag ccaggtgctc ccggtacgtc ctgtgccgaa
     9601 gatccagatc atgttctcgg gggcgatgcc gccaacggaa ggcccttcgg cgctggtccc
     9661 tgtcggatcg ggtaaggcag cgcgggtaac gccggcctcg aggccaagga cagccctgac
     9721 cctgcgcgcc acaccggaga ccttaggata tctgaacaca ggcaccagga gccccctacg
     9781 cgttgctccg gacggggtag agctccctga gtagagagcc tgtcaccact tccacaatct
     9841 cggcctgctc gggggtcagg tcctccctcc agcctcctgg cttcgccttg cggtggaacc
     9901 taccctcgcc cttctcctgc tctggtatgt tctcccaggc gttcttctcg acagatcgcg
     9961 cgagctcggc ttcctccact ggtatctcca gcgccgtgta gatgcgcttc atcgtcccca
    10021 gggtatccgc cctcaagtcc tcgtatctga cgaggacctt gagtcccctg tgggagtcgt
    10081 aggccaactt ggcatttccg acgtcccgca cgtaccttct ggcctgaccc ttcacgaagg
    10141 cgtccggcct gctctcggcc agcgaaggcg gcttctcctt accgcctttc ctcatcaggt
    10201 cggcggtcca ggtccctttc ttgtgggcgt cgaggttgga ggccacgacg tccctcggat
    10261 cgcggacgag caggatcata cgactctcgg gcagcgcctc catgagcagc ggagccccca
    10321 tcgagccctg cggctccttg atgatcaggt accttccgtc tttggctgct accccgggga
    10381 aacgcgcggc ggccgagtcg aggacgaacc tcctgagggt gccgagccag atctccctgt
    10441 agcgggcccc gaggatgaag tgctcatcct cgctgcgatg gcctgcccgc tcgtagtaga
    10501 ggttgccgaa cagatgcccc acaagcggct cgtgccacct gctgtacccc cctatctcgc
    10561 ccatgatggc gctgagccag gagcttcccg tcctcgccgt accgaatacc cagacgaggt
    10621 tctccggcct catgccgtcg gtctcctctc ctgctggcgg catcgctcta ctccttacct
    10681 tgcgccaggc atcccggatc acagcgcgcc ccgcgtggtc gccgtgtgag gatagaactc
    10741 gttcaggagc ggccccgtaa tctcctcgac gatctcgacc tgccttgcgg tcaggtcctc
    10801 cttccagcct cctggcttcg ccttgcgccg cttcttaccc tcacccttct cctgctctgg
    10861 tatgttctcc caggagttct tctcgacaga tcgcgcgagc tcagcttcct ccactggtat
    10921 ctccagcgcc gtgtagatgc gcttcatcgt ctccagagta tcggcgcgga ggtcctcgta
    10981 gcggaccatt gccttgtgac caccgtgggc tctgtaggcc tgcccggtct tctctatctg
    11041 ttgcacgtat gaattcgcgc gcatcctcac gtagacgttc gggttcctgt ccggcttcga
    11101 cgtacgtctc cgggcttccc ttcgctcctg aagccagctt ccctcgctac gggcgtccat
    11161 actcgaggcg acaacgtccc tggggtcccg gatcaggaag atcatacggc tctctggtag
    11221 ggcttccatg agcagcggcg agcctatgga gccgtttggc tcctttatga tcaggtatcc
    11281 accctccatc agctcgggga acctggcggc cgccccgccg aggaccagat ccctcatggg
    11341 cccgagccag gtctctttgt agggctctcc caggatgtat tgtctgcccg gcttgcgccc
    11401 ccccaccctg aagtagtgaa agttgccgaa gaggtttcct acgagcggct cgttccacat
    11461 ggcgcatccg tcgaggccct ccatcatgta gccgagccac gtgctgccgc tacggcccgt
    11521 gccgaagatc cagacgatgt tctcaggcct tacgccgcca ggcgatcgtc gggcccgcga
    11581 gcggagggac gcgccctttc tcagtgcgcg ccgccaggcc cgcttgaacc tcggcgtcaa
    11641 tattgttcct ccctcaggag ggcttgtcgg ccacaaggtg attgcaacga tctactccca
    11701 aaagacttat acatgcaatg ggccattata ctctcacaga accctgttta gtggcgccag
    11761 agtccccggt ctcggcgccg accgtcgcga tacacgagca tcttttcgcg caatgggtca
    11821 caaacccctt aacaatcctc cgttcttgta agataatgtg gccttgcgcg acagtatggt
    11881 cttctcgtag ggggatgttt gcgggtcaaa actatggtag tcggcgaggg tcgtcttcag
    11941 gggtatacct tcgccgctgc tctattatgg gcggtcctcc tactgatcct cgctccgggt
    12001 tcgaaggggg aacctgccgc ccagcctggg gtggacaacg atcctgaggg cgcgccttac
    12061 gtcgcagggg agctactcgt cgcctacgag cctggaacct ccgaggaggc cgaagagacc
    12121 gtcgtcaggc ggtcgggagc gcggaccctt gagaatctgc caggagaagt gagactcgtc
    12181 tccttcccgg cgatcaggcg cgaggcatcc gaggcggtgc gggagcgagc cctcgcacgg
    12241 gcactggcgt atctcgggca caaagcggcg gtcgaggccg ttgactacaa ctacttgcgc
    12301 gagccttact tcattccaaa cgacccgatg ctcgctaacc agtggggact catcagcgcg
    12361 aggttcggtg gcgcctggaa caacgcaaag ggcggtaggg cgaaggtagc tatcgtggat
    12421 tcgggcgcct actcgaatca tccagatata ggcaggatca tagcccagag agacttcgtc
    12481 gaggacgacg cggtggccga cgacgacaac gggcacggca cccacgtaac aggcatagcg
    12541 ggcgcgctca cggacaacgg caagggcgtg gctgggggat gcttcgattg cgaactcctc
    12601 atagccaag
//
LOCUS       DASTKM010000146         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_36404, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000146 DASTKM010000000
VERSION     DASTKM010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36404"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(410..1705)
                     /gene="odhB"
                     /locus_tag="VFQ10_06120"
     CDS             complement(410..1705)
                     /gene="odhB"
                     /locus_tag="VFQ10_06120"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET9964951.1"
                     /translation="MAAEIHVPELGESVADATVGRWLKQEGQAVKSGEALVELETDKI
                     NFEVEAEQDGVLESISKDEGETVNVGEVIGTIGEGAAPEEEQEEQPQAGEEREEQPQA
                     EQQEPEAEESGDETSNGHRELEDGAGRASPSVRKLAQEYEVDLAEVSGSGSGGRITRD
                     DVERLIREQSRPAAPEKTEVPERDGEAQPAPAASADGRAALEERVRVSRRRQTIAQRL
                     VESQQTAAMLTTFNEADMSAVMELRKRRKESFQERTGARLGFMSFFTKAAIGALKAFP
                     KVNAELQGSELILKKYYDIGVAVSTDEGLVVPVLRDADHKNFAEIEQGIADLATKARE
                     GKLTLDDLRGGTFTITNGGIFGSLVSTPILTLPQVAILGMHKIQERPVVVEGEVTVRP
                     MMYLALSYDHRVIDGAEAVSFLVRIKELVEDPESLLLEG"
     gene            complement(1723..>3268)
                     /locus_tag="VFQ10_06125"
     CDS             complement(1723..>3268)
                     /locus_tag="VFQ10_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9964952.1"
                     /translation="HAYREKFNKDFMIDLVGYRRFGHNEGDEPAYTQPMMYETIRDHP
                     TVREIWAEELERRGILDEGEAEAMFEEVMSDMAEVQKKPTDELKEEDFATDELYTPLV
                     EIPETAVAAERLSELNEALLEVPEDFTPNSKLERLFQKNRGDLDSIDWAHAEALAFAS
                     LLEDGTPVRLTGQDTERGTFSQRHAVLYNAETDERYVPLQNLPQSGASFSIYNSPLSE
                     IAVLGFEYGYSVNSEDALVLWEAQYGDFANVGQPMIDQFIVSGQAKWGQTSGLVLLLP
                     HGYEGQGPEHSSARLERYLQLAAQENVRIANLTTAAQYFHLLRAQAALKDNKRPLVIM
                     TPKSLLRHPMAASRLEDLSEGSFQPVLDDEATRERADSVERLILCSGKIYTELAGSEY
                     RDEAVDTAITRIELLYPFPEDRVREVIDGYPNLREILWVQEEPRNMGAWMFVEPLLRE
                     ISRGELPVLYVGKPPRPSPAQGSANFHKQEHAAVVRSAFENVGQDFDEVQAVEETQVR
                     ASAPSE"
BASE COUNT          505 a         1022 c         1037 g          704 t
ORIGIN      
        1 cctacgcccg cgtgggcttc ggcctcgtat acagcgcggc ctatatctac ggagcaggag
       61 atcggggtgt cctgttggct cgccttggct tcgagcgtta tccgggaaga tcccggctcc
      121 tcgcggtact tcttctttag cggcttctgc accgaccgga gatccaagaa tttcctcctg
      181 agatggctgc ccttctgcga cgtattgtag ccctcttaaa gcggtgtgcg gaaggacaga
      241 tccttccgca caccagtttc aggtatcagg caccaggctt caggatttcc ctggaaacag
      301 agttcgacca gcaggcaagg tgtactacct tgattgtcaa cggatccgga aaggggtgtg
      361 agacacaaag agacgagggg ctcccgaagg agcccctcgt ctcctgcctc tagccttcca
      421 gcagcagcga ctcggggtct tcgaccagtt ccttgatgcg gaccaggaag ctgaccgcct
      481 cggcgccgtc tatgacgcga tggtcgtagg agagcgcgag gtacatcatg ggacggaccg
      541 tgacctcgcc ttcgacgacg acggggcgct cctgtatctt gtgcatcccg agtatcgcga
      601 cctggggcaa ggtgaggatg ggcgtagaga ctagcgagcc gaagatgcca ccgttggtta
      661 tggtgaacgt tcccccgcgc aggtcatcga gtgtcaactt gccctcgcgg gcttttgtgg
      721 ccaggtcggc tatgccctgt tcgatctcag cgaagttctt gtggtcggcg tcgcggagga
      781 cggggacgac gagaccttcg tccgtggaga cggccacgcc gatgtcgtag tacttcttga
      841 gaatgagctc gctcccctgc agctccgcgt tgaccttggg gaaggccttt agcgccccga
      901 tggcagcctt ggtgaagaag ctcatgaagc cgagtcgcgc acctgtccgc tcctggaagg
      961 actccttgcg tcgcttcctc agctccatga ccgcgctcat gtcggcctcg ttgaaggtgg
     1021 tgagcatcgc cgcggtctgc tgcgactcga ccagcctctg ggcgatggtc tggcgtctgc
     1081 gggagacgcg cacccgctcc tccagcgccg ccctgccatc cgcagaagcg gccggcgctg
     1141 gctgtgcttc gccatcccgc tcgggtactt ccgtcttctc tggggccgcg gggcgggatt
     1201 gttcgcggat caaacgctcc acgtcgtcgc gggtgatgcg tccgccggaa ccgctgccag
     1261 agacttcggc aaggtcgact tcgtactcct gggccaactt tctgacagaa ggagaggcgc
     1321 ggccggcgcc gtcctcgagc tcgcggtgac cgttggacgt ttcgtcgccg gattcctcgg
     1381 cctcaggctc ctgctgttcg gcctgcggct gctcctcccg ttcttcccca gcctgcggct
     1441 gctcttcttg ttcttcctct ggggcggcac cttcgcctat agtgcctatg acctcgccga
     1501 cgttcacggt ctcgccttcg tccttggaga tggattcgag gacgccatcc tgctcggcct
     1561 cgacctcgaa gttgatctta tccgtctcta gctcgacgag cgcctcgcct gactttacgg
     1621 cttgaccctc ttgcttgagc cagcgcccga ccgtcgcgtc ggcgaccgac tccccaagct
     1681 cgggaacgtg tatctcagca gccactgctc actcttcctc tctcactcac tcggggcgga
     1741 ggcgcggacc tgcgtctcct ccacggcctg tacttcatcg aagtcctgcc cgacgttctc
     1801 gaaggccgac ctcacgacgg cggcgtgttc ctgtttgtgg aaattggccg agccctgggc
     1861 tgggcttggg cgcggaggct ttccgacgta gagtacgggc aactcgccgc gtgagatctc
     1921 acgcagcagc ggctcgacga acatccaggc gcccatgttc ctcggctcct cctggaccca
     1981 caagatctcg cgcaggttcg ggtaaccgtc gattacctcc cttactctat cctcggggaa
     2041 gggatagagg agctctatac gggtgatggc tgtgtccacc gcctcatccc tgtactcgct
     2101 gcccgccagc tccgtgtaga tcttgcccga gcacaggatg agccgctcga cggaatcggc
     2161 cctctcgcgc gtcgcttcgt cgtcgagcac cggctggaag gagccttccg agagatcttc
     2221 cagcctggag gcggccatgg ggtgtctgag gaggctcttg ggcgtcatga tgactagggg
     2281 acgcttgttg tcctttagcg cggcctgggc gcgaagcagg tggaagtact gggccgccgt
     2341 tgtgaggttc gctatgcgca cgttctcctg cgcggcgagc tggaggtaac gctccaggcg
     2401 cgcgctcgag tgctcgggtc cctggccctc gtagccgtgc ggcaggagta gtacaagccc
     2461 cgaggtctgg ccccacttcg cctggccgct cacgatgaac tggtcgatca tgggctgtcc
     2521 gacgttggcg aagtccccgt actgcgcctc ccagaggacg agcgcgtcct ccgagttgac
     2581 cgagtaacca tactcgaagc cgagcacggc gatctcggag agcgggctgt tgtagatcga
     2641 gaacgaagcg cctgactggg gtaggttctg gagtggcacg tagcgctcgt cggtctcggc
     2701 gttatagagc acggcgtggc gctgggagaa ggtcccgcgc tccgtgtcct gccctgtgag
     2761 gcggacaggg gtgccatcct cgagtagcga ggcgaaggcc agggcttctg cgtgcgccca
     2821 gtctatggag tcgagatctc cgcggttctt ctggaagagg cgctccagct tggagttcgg
     2881 cgtgaagtcc tcggggacct cgagtagggc ttcgttcagt tcagagagtc tctcggccgc
     2941 gacggcagtc tcggggatct cgacgagcgg cgtgtacagc tcgtccgtgg cgaagtcttc
     3001 ttctttgagt tcatcagtgg gcttcttttg aacctccgcc atgtcggaca tgacctcctc
     3061 gaacatggcc tcggcctcgc cctcgtccag gatgccgcgg cgttcgagtt cttcggccca
     3121 gatctcacgc accgtggggt ggtcgcggat ggtctcgtac atcatcggct gcgtataggc
     3181 aggttcgtcg ccctcgttgt gcccaaagcg gcggtagccg acgaggtcga tcatgaagtc
     3241 cttgttgaac ttctcgcggt aggcgtgg
//
LOCUS       DASTKM010000147         2839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_742581, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000147 DASTKM010000000
VERSION     DASTKM010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742581"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFQ10_06130"
     CDS             <1..600
                     /locus_tag="VFQ10_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9964953.1"
                     /translation="ETIMNLTNPYEGERRPGTVGRPFPGQEARVVDVETREPLAEGEI
                     GEIEVRGPNVFPGYWNRPDATEESFDAEGWFATGDLGSVGEDGYFTISGRAKELIISG
                     GFNVYPREVEDVIEGCPGVSEVAVVGLPDPEFGESVVAVVVPEDPGLTAEKVTSFCRE
                     ELASYKKPREVVFVDDLPRNALGKVLKHRIREGLSEPEE"
     gene            604..1236
                     /gene="dhaL"
                     /locus_tag="VFQ10_06135"
     CDS             604..1236
                     /gene="dhaL"
                     /locus_tag="VFQ10_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HET9964954.1"
                     /translation="MDATQATSDVVREMAATMEENRRYLTKLDSEIGDGDHGNNMNRG
                     FQAALDRLEGADPSTPADVLKAVSMALISKVGGAAGPLYGTAFLRASSVLGDKEEISA
                     EDAAEALEAALGGLKQRGKAEVGDKTIVDALEPAVEAAKEAASEGSVTAVFRSAADAA
                     KEGAESTVPLTAKRGRASYLGARAAGHQDPGATSTYMLLDAAARALESSS"
     gene            1236..1631
                     /gene="dhaM"
                     /locus_tag="VFQ10_06140"
     CDS             1236..1631
                     /gene="dhaM"
                     /locus_tag="VFQ10_06140"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HET9964955.1"
                     /translation="MVGIVLVSHSKEIADGTADLVLQMAGEVEISAVGGDADGSFGTD
                     PERIEASIRDLQADEVLVFMDLGSAVLSAETVLEMLPSERRERVTLVDAPFVEGAFAA
                     GVTASTGADAEECVEAAMEARTEPKLQDG"
     gene            1705..2214
                     /locus_tag="VFQ10_06145"
     CDS             1705..2214
                     /locus_tag="VFQ10_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9964956.1"
                     /translation="METNRGSQGILSEEMAEKRKEIVELLKTAYFMELETVMNYVTNS
                     INPDGIRAQEVKESVEEDIQEELTHAQQFAARIKELYGVVPGSMEFKASQDTLQPPKD
                     QVDVVHVIKGVIDAEVGGIQHYTRIVEETEGVDPVTQDMVIDILHDEQGHRRLFEGFL
                     REYEAEGLA"
     gene            2401..>2839
                     /locus_tag="VFQ10_06150"
     CDS             2401..>2839
                     /locus_tag="VFQ10_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HET9964957.1"
                     /translation="MPEPLLSSFNVFLALVLVGLNGFFVAAEFALVRVRESRIVQLEQ
                     EGSRRASIVRQTLRDLDSYLSVCQVGITVASLALGWVGEPAVSHLIRPVLEAVGIANE
                     RIVSIIAIFLGFSAITYAHLVFGEQAPKYFSIQKAEKVSLWISR"
BASE COUNT          575 a          817 c          966 g          481 t
ORIGIN      
        1 gagacgatca tgaacctcac caacccctac gaaggcgagc gcaggcccgg taccgtggga
       61 cggcctttcc ccggccagga ggcgcgggtc gtcgatgtgg agacgcgcga gcctttggcc
      121 gaaggggaga tcggagagat cgaagtccgc ggccctaacg tcttccccgg ctactggaac
      181 aggcccgatg cgaccgagga gagcttcgac gcagagggct ggttcgccac gggggatctc
      241 ggttccgtag gagaggacgg ctacttcacc ataagcgggc gggccaaaga actcataatc
      301 agcggcggtt tcaatgtcta cccccgcgag gtcgaggatg tgatcgaagg ttgtccaggg
      361 gtgtctgaag tggccgtcgt cgggctgcct gaccccgagt tcggagaaag cgtggtcgcc
      421 gtcgttgtgc ccgaagatcc tggactcacc gccgagaagg tgacgagctt ctgccgtgag
      481 gagctggcca gctacaagaa gccccgcgag gtggtgttcg tcgatgacct tccgcgcaac
      541 gccctcggga aggtactcaa gcacaggata agggaaggat tgagcgaacc ggaggagtag
      601 gatttggacg cgacgcaagc caccagcgac gtagtgaggg agatggccgc caccatggag
      661 gagaacaggc ggtacctgac caagctagac tcggagatcg gggacggcga ccacggcaac
      721 aacatgaacc gcggcttcca agccgcgctc gatcgtctgg aaggcgccga cccctcgacc
      781 cctgccgatg tgctgaaagc cgtctccatg gccctgatca gcaaggtcgg cggggccgcc
      841 ggcccgctct acggcacggc tttcctgcgt gcctcgtcgg tgctcgggga caaggaagag
      901 atctcggccg aagatgctgc cgaggctctg gaggctgccc tcggtggcct aaagcagcgc
      961 ggcaaggccg aggtggggga caagacgatc gtcgacgccc tcgaacccgc cgtcgaagca
     1021 gcgaaagagg ccgcctccga ggggagcgtg accgccgtgt tcaggagcgc ggcggatgcc
     1081 gcgaaggagg gtgccgagtc tacggtcccg ctcacggcga agagggggag ggcgagctat
     1141 cttggggcca gggccgcggg gcaccaggac ccgggggcga cgtctacgta catgctgctg
     1201 gatgccgcgg cgcgggccct ggaaagctcc tcctgatggt cgggatcgtg cttgtctcgc
     1261 acagcaagga gatcgccgat ggcacggctg acctggtctt gcagatggca ggagaggtcg
     1321 agatctcagc cgtcggaggt gatgccgatg gctctttcgg cacggacccg gagaggatcg
     1381 aagcctccat acgcgacctc caagccgacg aggtgctcgt gttcatggac ctcggcagcg
     1441 ccgtcctctc cgcagagacc gtcctggaga tgctgccctc ggagagaaga gaaagggtca
     1501 ccctggtcga cgctcccttc gtcgaagggg ccttcgcggc cggggtcaca gcatcgaccg
     1561 gggctgacgc tgaggagtgc gttgaggcgg cgatggaggc ccgcacggag ccgaagctgc
     1621 aggatgggta gagggtttaa tcggggggtg cgagggtaca ttctccgtgt ggatcgcatg
     1681 agatctaata ggagggcata gcgcatggag accaatcgcg gcagccaggg cattctttcc
     1741 gaagagatgg cggagaagcg caaggagatc gtcgagctgc tgaagacagc ttactttatg
     1801 gagctggaga ccgtcatgaa ttacgtgacc aactccataa accctgacgg catccgcgcc
     1861 caggaggtca aggagagcgt ggaggaagac atacaggaag agcttaccca cgcccagcag
     1921 ttcgcggccc gcatcaagga gctctacggt gtcgtgccag gctcgatgga gttcaaggca
     1981 tctcaggaca cgctgcagcc gccaaaggac caggtagacg tagtacacgt cataaagggc
     2041 gtcatagacg cggaagtcgg cggcatccag cactacacgc gcatcgtgga ggagactgag
     2101 ggggtagatc ccgtcacaca ggacatggtt atagatatcc tgcacgacga acaggggcac
     2161 aggcgccttt tcgaaggctt cctgcgcgag tacgaagcgg agggcctggc ttagagatag
     2221 cgttcaggaa acgccctacc gagaggcccg gcgacgaagg tcgggcctct tttcatgctg
     2281 tgggatttgt gcgggtcccc ggctgcggta taatccgccc cgtaacgcga tggacgaacc
     2341 tcacgctaaa cgctaccgac gaaaaccctg cggcccgacc gccggtgggc tcgcgtagaa
     2401 gtgccggaac cccttttatc ctcgtttaac gtctttctgg ccctcgtcct ggtaggcctg
     2461 aacggtttct ttgtggctgc cgagttcgcg ctggtgcgcg tgcgggagtc gcgcatcgta
     2521 caactagaac aggaaggtag caggcgggcc tcaatagtac gccagacgct gcgcgacctg
     2581 gactcctatc tctccgtctg tcaggtaggg attacagtag cttccctggc gctaggttgg
     2641 gtcggcgagc ctgccgtctc gcacctcatc cgtcccgtgt tggaggctgt aggaattgcg
     2701 aacgagcgga tcgtctccat catcgccatt ttcctaggct tctcggccat aacctatgcc
     2761 cacctggtct tcggggagca ggcgcccaag tacttctcca tccagaaggc cgagaaggtg
     2821 tcgctctgga tcagccgtc
//
LOCUS       DASTKM010000148         5933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_214594, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000148 DASTKM010000000
VERSION     DASTKM010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214594"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VFQ10_06155"
     CDS             complement(<1..1319)
                     /locus_tag="VFQ10_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9964958.1"
                     /translation="MSTLNLGIVAHVDAGKTTLTERLLFAAGVIDEIGRVDDGNTQTD
                     TLTLERQRGITIKSAVVSFVIDDVTVNLIDTPGHPDFIAEVERVLGVLDGAVLIISAV
                     EGVQSQTRLLMRTLQRLRVPTLIFVNKIDRGGAKCERLLRSIAERLTPAIIPMGSASG
                     LGTRSPSFTPYGAADADFTSGLVDMLADHDDAILAAYVEDDATVSYRRLRGELAAQTG
                     HALVHPVFFGSAVTGAGVDELIAGIKELLPAAEDETDGPVSGTVFKVERGPAGENIAY
                     VRMFSGTVTVRDRLQLGGDEQEKVTAISVFDRGSAVQRASVAAGRIGRLWGLGEVRIG
                     DAIGEPRTTPERHYFAPPTLETVVVPRRPADKGALHVALTQLAEQDPLINVRQDDSRQ
                     GIFVSLYGEVQKEVVGATLANDYDIDVEFRETTMICIERPIGVGAAV"
     gene            complement(1495..2664)
                     /locus_tag="VFQ10_06160"
     CDS             complement(1495..2664)
                     /locus_tag="VFQ10_06160"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9964959.1"
                     /translation="MKLARRMERLGTETAFSVLAKAKALEAEGREIIHLEIGEPDFDT
                     PEHVVEAGCKALRDGHTHYTPTAGIPELREAIAVDVARSRGIDVDPEQVVVTPGGKPI
                     MFFTILALVEGGDEVLLPNPAFPIYESMVNFVGGRPIFVPLRQENGFRFDLDEFRAGF
                     SERTSLVILNSPANPTGGVLTADDIAGLADILRERPDVYVLSDEIYSRLLYTDEFASL
                     ASEPGLGPDGRTIILDGFSKTYAMTGWRLGYGVMPAPLAEEVSKLQVNSNSCTSAATQ
                     HAGLAALQGPQDAVGSMLAEFRARRDLIVDGLNELPGVECITPQGAFYAFPRIIETGH
                     PADKLADLLLDDAGVACLSGTAFGYHGEGHLRLSYANSRENISKALQRMGEALSR"
     gene            2906..3109
                     /locus_tag="VFQ10_06165"
     CDS             2906..3109
                     /locus_tag="VFQ10_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964960.1"
                     /translation="MIRVCIEVDSGKASPTTLFVKAHSILRALEVAEERNPDCALNVV
                     FPLDPHTFFVRDAPEGVEAETAA"
     gene            complement(3215..3868)
                     /locus_tag="VFQ10_06170"
     CDS             complement(3215..3868)
                     /locus_tag="VFQ10_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9964961.1"
                     /translation="MATNWDVRFFQSTRGRIVTLLRRSKSTVEDLARALDLTDNGVRA
                     HLGVLERDGIVRQRGSLRRRSGGGKPAYVYELTSEGEELFPKAYEPTLGRLLDVLSER
                     LGPEESEALLRSVGHRMVEGKTVPTDGARARLEAAAGVLNELGGLAELEEQDGTLVIR
                     GYGCPLAAVTPDHPEVCRLAEALVAEVAGVPVHERCDRSERPRCRFEVAPADDATKR"
     gene            4087..4317
                     /locus_tag="VFQ10_06175"
     CDS             4087..4317
                     /locus_tag="VFQ10_06175"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HET9964962.1"
                     /translation="MPDQTKITALDNGPYLVKGPVLLVDAEGSEFRAERATVALCRCG
                     GSMTKPFCDGTHSKIGFRAAQRAVREEVREGA"
     gene            4314..5015
                     /gene="ribA"
                     /locus_tag="VFQ10_06180"
     CDS             4314..5015
                     /gene="ribA"
                     /locus_tag="VFQ10_06180"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET9964963.1"
                     /translation="MIRTATLGYSVHGSDYDVTEGAILQGPARELGETCETLVKCAVE
                     TRLPTPFGEFRLRAYENELDDLTHLALLMGGLEERENVLVRVHSACLTGDALHSLRCD
                     CGDQLEAAMQRVAAEGKGVIIYMQQEGRGIGLLNKLKAYNLQDEGLDTVEANQRLGFA
                     PDLRDYGIGAQILKDLGLMRIRLLTNNPTKVVGLRGLGFEITERVPIEIEPNEYNECY
                     LKTKREKFNHVFEKL"
     gene            5733..>5933
                     /locus_tag="VFQ10_06185"
     CDS             5733..>5933
                     /locus_tag="VFQ10_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964964.1"
                     /translation="MTIYHPWESGTENSPRWDAALEPVEAGEVSPHERYDLEHVDDPS
                     QRPTDADYDRYLWLVKLVKSAAC"
BASE COUNT         1144 a         1826 c         1851 g         1112 t
ORIGIN      
        1 tcgacggccg caccgacgcc gatgggccgt tcgatgcaga tcattgtcgt ttctcggaac
       61 tcgacgtcga tgtcgtagtc gttagccagc gtcgccccga cgacctcttt ctgcacctca
      121 ccgtagagcg agacgaatat cccttgcctg ctgtcgtcct gccgcacgtt gatcaggggg
      181 tcctgctcgg cgagttgggt gagcgcgacg tgcagcgcgc ctttgtcggc ggggcggcgg
      241 ggaacgacga ccgtttccag cgtcggcggg gcgaaatagt ggcgctccgg ggttgtccgc
      301 ggttcgccga tcgcgtcccc gatccgaact tccccgagac cccagagcct gccgatccgt
      361 cccgcggcga ccgacgcgcg ctggacggcc gagccgcggt cgaagacgct gatcgcagtg
      421 actttctcct gctcatcacc gccgagttgc agccggtccc gcaccgttac cgtccccgag
      481 aacatgcgga cgtaagcgat attctccccg gccggaccac gttcgacttt gaaaaccgtg
      541 cccgagaccg ggccatccgt ctcgtcttcg gcggcgggca gcagctcttt gatgccggcg
      601 atcagctcat ccacgcccgc gcctgtgacg gccgagccga agaacacggg atgcacgagc
      661 gcatgcccgg tctgcgccgc aagctcgccg cggagtcggc ggtacgagac cgtcgcatcg
      721 tcctcgacgt acgcggcgag aattgcgtcg tcgtggtcgg ctagcatatc gaccagtccg
      781 gacgtaaagt ccgcgtcggc ggcaccgtaa ggcgtgaaac tgggactgcg ggttcccaac
      841 ccgctggccg atcccatcgg gatgatcgcc ggcgtcagtc tctcggcgat gctccgcagc
      901 aagcgttcac atttcgcgcc gccgcggtcg atcttgttca cgaagatgag ggtgggaacg
      961 cgcagccgtt gtagcgtcct catcaacaag cgggtctgtg actgcacgcc ctcgaccgcg
     1021 gagattatca gcacggcgcc gtcgagcacg cccaacaccc gttccacctc ggcgatgaaa
     1081 tccgggtggc cgggggtgtc gatcaggttg accgtgacgt cgtcgatgac gaacgatacg
     1141 acggcagact tgatcgtgat gccacgttgc cgttctagcg ttaaggtatc ggtttgggtg
     1201 ttcccgtcat cgaccctgcc gatctcgtcg atgacaccgg cggcaaagag cagccgctcg
     1261 gtcagtgtcg tcttaccggc gtctacatgc gccactatcc ccaggttcaa agtgctcata
     1321 tagcgtcatg tcctctagat aggtcgtaac tccctccctg gtagacatga acgctcggcg
     1381 catttcctct ccttcgtcga tcggctgttg ctaggagtgt agcagaaggc ccctgaggat
     1441 aaccgattcg ctgggtggat cagctggctt ctctgagcga cgaagatccc gcgatcaccg
     1501 cgagagggcc tcacccatcc tttggagcgc tttgctgatg ttctcgcggg aattggcgta
     1561 agagaggcgc agatggccct ccccatggta gccgaatgca gtaccggaga ggcacgccac
     1621 acccgcgtcg tcgaggagta gatcggcgag tttgtcggcc gggtggccgg tctcgatgat
     1681 gcgcgggaag gcgtagaatg ctccctgggg cgttatacat tcgacaccgg gcagttcgtt
     1741 gaggccgtct acgatcaggt ccctgcgcgc cctgaactcg gcgagcatac tccccacggc
     1801 gtcctgcgga ccttggaggg ccgcgagccc ggcgtgttgt gttgccgcgc tggtgcatga
     1861 gttggagttg acctggagct tggatacttc ctcggcgagg ggcgctggca tcacgccgta
     1921 gccgagccgc cagcctgtca tcgcgtaggt cttggagaag ccgtctagga tgatcgtccg
     1981 cccgtcaggg ccgagtccgg gctccgaggc taggctggcg aactcgtctg tgtagagcag
     2041 ccgagagtag atctcatcgc tcaagacgta gacgtcgggg cgctcgcgca ggatgtctgc
     2101 gagcccggcg atgtcatcgg ccgtaaggac gccgcccgtc gggtttgccg gggagttgag
     2161 gatcaccaga ctggtgcgtt cgctgaagcc cgctctgaac tcgtcgaggt cgaagcggaa
     2221 cccgttctcc tggcgcagag gtacgaagat gggcctcccg ccgacgaagt tcaccatcga
     2281 ctcgtagatc gggaacgccg ggttgggcag aagcacctcg tcacccccct ccacgagcgc
     2341 caggatggtg aagaacatta tcggctttcc gccaggcgtg actactacct gttcgggatc
     2401 gacatctata cccctgctac gcgccacatc aacggcgata gcctcgcgca gctctggaat
     2461 accggcggtg ggggtgtagt gggtgtgccc gtcgcgcaga gctttgcaac cggcctcgac
     2521 gacatgctcc ggcgtatcga agtcgggttc gccgatctcg aggtggatga tctcacggcc
     2581 ctccgcctcc agggctttcg ctttcgccag gaccgaaaaa gccgtctccg ttcccagacg
     2641 ctccatccgc ctcgccagtt tcacaccgtc ctccgctcga tcgtgtccag gcaagccccc
     2701 gcattacacc acacgcgtag gtccatgaat gtgtagcgaa gggcgtcgga tacggcgatg
     2761 cgccggcgat ctctggtgcg tctgatccag gctagcaccc tgaacgtacg tattgttgcc
     2821 tacattttgc gccgctgtta ccgatatgta acagaagcgg tgtagaaggg gtatagagcg
     2881 tgtcgttggt agatggagga tccgaatgat aagggtgtgt atcgaagtag atagcggcaa
     2941 ggccagcccc acaacgttat tcgtgaaggc ccacagcatc ctgcgggccc tcgaagtggc
     3001 ggaggaacgg aaccctgatt gcgccttgaa cgtggtgttc cctctggacc ctcatacttt
     3061 ctttgttcgg gatgccccgg aaggggtcga agccgagact gcagcttagc ctgccaggtc
     3121 tattccttct aatctcctgc gacgggattg cacgaggcgc atcgcgaggt gggcagactt
     3181 gacatctaga tcgatctgac gccggcaagc gcaactatct tttggtagcg tcatcggccg
     3241 gcgccacctc gaagcggcag cgcggcctct cgctacggtc gcagcgctcg tggacgggca
     3301 cccccgcaac ctcggctacc aacgcttctg ccaagcgaca tacctcgggg tgatccggcg
     3361 tcacggcggc cagtggacag ccgtagcccc ggatgacgag agtgccatct tgctcttcga
     3421 gctccgcgag accacccagc tcgttgagca caccggcggc tgcctcgagc cgggcgcgag
     3481 cgccgtctgt cggtacggtc ttgccttcca ccatacgatg acctaccgac ctaaggaggg
     3541 cctccgattc ctcaggccca agccgctcgg aaagaacatc cagcagccgg ccaagtgtgg
     3601 gttcgtaagc cttggggaag agctcttcac cctccgatgt aagctcgtag acgtaagcag
     3661 gcttcccgcc gccacttctg cggcgcagcg aacctcgctg gcgcacgatg ccgtcgcgct
     3721 cgaggacgcc gagatgcgcg cggacgccgt tgtcggtgag atccagcgcc ctagcgaggt
     3781 cctccacagt gctcttggac cgtctgagca gcgtgacgat ccgccctcgc gtgctttgga
     3841 agaacctcac gtcccagttc gtagccaata gtcactccct ccgctggtca gcttcagtat
     3901 agcaagtaaa ctaaattatt ctattatgtc aattttatac ttgacataat atagaaaagg
     3961 catgtagact gctgtgaggg caatggcaac gacctttagg gaggtagaaa tgggatctgt
     4021 ggcacctaat cccgtagcgt cggggcgggt tctggcgaag ataactacag aacgaggagg
     4081 ataaccatgc ctgatcaaac gaagatcact gcgctcgata acggaccgta cctggtgaag
     4141 ggcccggtct tgctcgtcga cgccgagggc agcgagttcc gcgccgagag ggcgaccgtg
     4201 gcattgtgcc ggtgtggtgg ctcgatgacc aagccgttct gcgacggcac gcactcgaag
     4261 attgggttcc gtgcggcgca gagagcggtt cgcgaagaag taagggaggg agcgtgattc
     4321 gaaccgctac tttgggttac agcgtgcatg gctctgatta cgacgtgacc gagggggcga
     4381 tactccaggg gcccgctcgg gagcttggtg aaacctgcga gactctggtc aagtgcgccg
     4441 tcgagacccg ccttcccacg ccgttcggcg agttcaggct tagggcttac gagaacgagc
     4501 tggatgatct cacgcacctg gcccttctca tgggcgggct ggaggaaagg gagaacgtgc
     4561 tggtgcgcgt ccattccgcc tgcctcacgg gggacgcgct ccattctctg cgctgcgact
     4621 gtggagacca gctggaggcg gcgatgcagc gcgtggccgc agaggggaaa ggtgtgatca
     4681 tctacatgca gcaggagggc agggggatag ggctcctcaa caagttgaag gcttacaacc
     4741 tccaggacga gggtctcgac accgtggagg ctaaccagag gctcggcttt gcaccggact
     4801 tgagggacta cgggattgga gcacagatcc tcaaggacct tggcctcatg cgcatccggc
     4861 tgctaactaa caacccgacc aaggtggtcg gcctgagggg tttggggttc gagatcaccg
     4921 agcgggtgcc aatcgagatc gaacctaacg aatataacga gtgctacctc aagaccaagc
     4981 gcgagaagtt caatcacgtc ttcgagaaac tctaacgagt cgcaacacag cacggaagaa
     5041 agcattctga ggagtatgtc agatgcctta cggccttgtg atggccaacc tgaatgagta
     5101 cccagcagca tcccggtctc cctctcgccg cggcgaagag gaatctcact acggaagacc
     5161 agagcatcgt aaacacaaac cggccgacca aatggaggcg aggcaatgca ggtagagatc
     5221 ttcccaagaa gcgtgctcct ggaacgcgaa ggattggccg cgagagcaca agatatcctt
     5281 gatcagaacg acatgggggg tggacgaggg cggcgacgga cctctacccc caccagtgga
     5341 gctgggacag cggattcatc gccatagggc tggcgcacct cgataccggt cgcgccgccc
     5401 gagagctgct cacgctattt cggcatcagt ggaaaaccgg caaggttccc cacatcgtct
     5461 tcaatcccga ggccccgccg gacagctact tccccggcgc tgagcactgg gcctgtgccg
     5521 cagcctctcc cgaggcacct cccgccccgc cctacacaag ctgcctgtgc cagccgccaa
     5581 cccacgcaat cgccgctcag aggatctggg aagccgccag caacgggagg gctcgcgcgg
     5641 aagctaccgc cttcctgcgc gacatctacc ccaagctcct ctcctggcac cgctacctcc
     5701 tgacctaccg ggatccggag gagtccggac tggtgacgat ctaccatccg tgggagagtg
     5761 gcaccgagaa ctcccccagg tgggacgcgg ctctcgagcc ggtggaggca ggcgaggtgt
     5821 caccgcacga gcgctacgat ctcgagcacg tagacgaccc ttctcagagg cccaccgacg
     5881 cggattacga tcgctacctc tggctcgtga agctggtaaa gagtgccgcc tgc
//
LOCUS       DASTKM010000149         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_214797, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000149 DASTKM010000000
VERSION     DASTKM010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214797"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..>2443
                     /gene="dnaE"
                     /locus_tag="VFQ10_06190"
     CDS             35..>2443
                     /gene="dnaE"
                     /locus_tag="VFQ10_06190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET9964965.1"
                     /translation="MHCHSEYSMLDGASRIGDLVSFAKDQGMPGLALTDHGVMYGAVK
                     FYKEAREAGIKPLMGCEVYVTADRNDRSRAPYYHLTLIARTAEGYRNLMKLSTCGFLE
                     GFYYKPRVDMEMLRKYGRGIICLSGCLSAEVPTRILEGRSEEARKLVLQYAEIFDDVY
                     LEMQDHLEIPDQRRVNEGLIRLHAETGLDLVATNDSHYTTRNDARMHDVLLCIGTGKF
                     FNDQNRMSFSGQEFYVKPAEEMAALFPNHPEALENTIKVVESVEDVGIELGKTRLPNF
                     PKPEGYTADQYLREQCERGLARRYGGRAKSREVLSRLEFELETIGKMGFADYFLIVWD
                     FVKYAKDRKIAVGPGRGSAAGSIVAYALEITDLDPLEYSLLFERFLNPDRINMPDVDI
                     DFSVSGRSEVMRYVTDKYGGHEHVAQIITFGTIGAKAAIRDSGRIYQYPYADTDKLAK
                     FIPEKPVGTTLRDVLRPENGSYVAGDKHPGPAREIIKFVQQDEAAQQVLDTAFEIEGF
                     ARHAGTHAAGVVISEEPLTDIVPLQRVARDEGSVMVQHPMSDVEALGLLKVDFLGLRN
                     LDVIEETLETIRRGGGEEPDIRSIPLDDEKTLKLFARGDTFGVFQFESSGMQRMLQEV
                     RPDRFDDLVAMNALYRPGPMDYIPTFKRGKHDPESVRYLDERLKPILEPTYGVAAYQE
                     QLMEISKSLGGFTPGEADTLRKAIGKKNAAMLATLKDKFMAGCAESGVSTHGAEELWN
                     WMEKAGGYSFNKSHSACYSFLAFQTAYLKAHYPEAYMAALMSSVMNTKDRVPQYVAEA
                     RAMKI"
BASE COUNT          521 a          718 c          779 g          425 t
ORIGIN      
        1 gttggcggtt acgtcgggcg gcgctttcgc gcacttgcac tgtcactctg agtattcgat
       61 gctcgacggg gcgagccgca taggggacct cgtctccttt gcaaaagacc aggggatgcc
      121 tgggctcgcg cttaccgacc acggtgtgat gtacggcgcg gtcaagttct ataaggaggc
      181 cagggaggcc gggataaagc ccttgatggg ctgcgaggtc tacgtcacgg ccgaccggaa
      241 tgaccggagc cgcgcccctt attaccacct tacgctcatc gccaggaccg ccgaaggcta
      301 caggaacctc atgaagcttt cgacctgcgg gtttctggag ggcttctact acaagccgcg
      361 ggtcgacatg gagatgctga ggaagtatgg gcgtgggatc atttgcctct ccggctgcct
      421 ctcggccgag gtgccaacca ggatcctgga ggggcgttcg gaggaagcgc gcaagctggt
      481 actgcagtac gcggagatct tcgatgatgt atatctggag atgcaggatc atctggagat
      541 accggatcag aggcgtgtga acgaggggtt gatcaggctg cacgcggaga ccggcctgga
      601 cctcgtcgct accaacgatt cccactacac cacgagaaac gacgccagga tgcacgatgt
      661 actcctgtgc atcggtacgg gcaagttctt caacgaccag aaccgcatga gcttcagcgg
      721 gcaggagttc tacgtcaagc ccgccgaaga gatggccgcg ctctttccaa atcacccgga
      781 ggcgctggag aacacgatca aagtcgtcga gagcgtcgag gacgtcggga tcgaactggg
      841 caagacacgc ctgccgaact tccccaagcc agaagggtac accgccgacc agtacctgag
      901 ggagcagtgc gagcggggcc tggcgaggcg ttacggcggg cgggcgaaga gccgggaagt
      961 gctcagcagg ctcgagttcg agttggagac cataggaaag atgggcttcg ccgactactt
     1021 cctgatcgtc tgggattttg tcaagtatgc gaaggacagg aagatcgcgg tcgggcccgg
     1081 aaggggatcg gcggccggat ccatcgtcgc ctacgcgctg gagatcacgg acctcgatcc
     1141 cctcgaatac tctctgctct tcgagcgttt cttgaaccca gaccgcatca acatgcccga
     1201 tgtggacata gacttctcgg tctcggggcg ctcggaggtg atgcgctacg tcacggacaa
     1261 gtacggcggc catgagcacg tcgcccagat catcacgttc ggtacgatcg gcgccaaggc
     1321 tgccatcagg gactcaggac ggatctacca gtatccctac gcggacacgg acaagctcgc
     1381 caagttcatt cccgagaagc ccgtcgggac cacgctccgc gacgtgcttc ggcccgagaa
     1441 cggctcttac gtcgccgggg acaaacaccc cggtcctgcg cgcgagatca ttaaattcgt
     1501 acagcaggac gaggccgcgc agcaagtact cgacactgcc ttcgagatag aggggttcgc
     1561 ccgccacgcg gggacccacg ctgccggcgt ggtcatctcg gaggaaccgc tcaccgacat
     1621 agtgccgctg caacgcgtcg ccagggacga ggggtcggtc atggtccagc atcccatgtc
     1681 cgacgtggaa gcgttgggat tgctcaaggt ggatttcctc ggcttgagga acctcgatgt
     1741 gatcgaagag accctcgaga cgatccgtag gggcggcggc gaggaacctg acatccgttc
     1801 gatccctctc gacgacgaga agacgctgaa gctcttcgcc aggggagata ctttcggggt
     1861 cttccagttc gagtcgagcg ggatgcagcg gatgctccag gaggtcaggc ccgaccgctt
     1921 cgacgacctg gtcgccatga acgccctata caggcctggt ccgatggact acatccccac
     1981 cttcaagcgc ggcaagcacg atcccgagag cgtcaggtac ctggacgagc gactaaagcc
     2041 gatcctggag cctacctacg gcgtcgccgc ctaccaggaa cagctgatgg agatctccaa
     2101 gtctctgggg ggatttaccc ctggcgaggc cgacacgctg cgcaaagcca taggcaagaa
     2161 gaacgcggcg atgcttgcga cgctcaagga caagtttatg gctggatgcg ccgagagcgg
     2221 cgtctccacg cacggcgccg aggagctctg gaactggatg gagaaggccg gaggatactc
     2281 tttcaacaag agccacagcg cgtgctactc gttcctggcc ttccagaccg catacctcaa
     2341 ggcccactat cccgaggcgt atatggcggc cctcatgagc agcgtgatga acaccaagga
     2401 cagggtgccc cagtacgtcg ctgaggccag ggcgatgaag ata
//
LOCUS       DASTKM010000150        22812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_743478, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000150 DASTKM010000000
VERSION     DASTKM010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 22812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22812
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743478"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VFQ10_06195"
     CDS             complement(<1..1364)
                     /locus_tag="VFQ10_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9964966.1"
                     /translation="MKAEKQAVTGGPERGRSQKKILLTLVVPTRNEAENVPMLVHELR
                     ESLSDLDYRVVFVDDSTDETPEVIRGLAEQDGRILLVRREGAERYGGLSTAVTTGMEL
                     FSGASEYTCVMDADLQHPPEKVRKMLEIARRSDADVVVASRYGQGGYAGLSGRMRRAV
                     SVGSKYLARILFKEARKTNDPMAGFFLIKNQAISGIQFRPTGFKVLLEILVCAPELKV
                     LETPFEFQVRHAGVSKATIRQGFEYLSHILSLFWYVPSAGRFWKFALVGASGVLVNMV
                     TLIVLAEYFHAHKVIAWMFAVGLSILSNFLLNNAFTWRDIRHSSRIHFFLRGVLAYPV
                     AVMGIGANFAVWYPLVKYVSDDFPYYALCNLLGIAAGTSVNFILSSQLVFRPSTPKSL
                     DPHAPPKQIAEEVRRELKADRVALIRVPDLTPLGENSPRDLTASDRSVIDLVVKTTHP
                     TLTMT"
     gene            1449..1733
                     /locus_tag="VFQ10_06200"
     CDS             1449..1733
                     /locus_tag="VFQ10_06200"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET9964967.1"
                     /translation="MQNSDLQRAHVRVSGQVQGVFFRDSTRQKAEELGLAGWVKNVPE
                     GQVEALFEGPSDKVREMVRWCEEGPQRASVKNVDTNFESAEGDLEGFQVR"
     gene            1730..2668
                     /locus_tag="VFQ10_06205"
     CDS             1730..2668
                     /locus_tag="VFQ10_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964968.1"
                     /translation="MNPGTELPPDLRSLLDREGVEIVGSRAIDHGTQYELARDDETAK
                     LNVYRTGKISAGGRASGLRELLEGWRTSQTSVGGTRASTARAGGRPILDGTPRLGIDE
                     AGKGDYFGPLVVAGVRITHAETARKLQEIGVRDSKEIRASGISRISGRILEAVGRNNV
                     HVISLPPPEYESSRTAAGNVNRLLGELDAKITGKLVDEVEVVVVDEFDRSARSYLVPF
                     VPDGIRLEVRPRAEDDAAVAAASIVARARQLEEIDRLSERVGFGLPLGATHVVEAGRR
                     VVEELGEDGLAEVAKIHFATTRRVLKTAEQSDGGDP"
     gene            2665..3684
                     /gene="ltaE"
                     /locus_tag="VFQ10_06210"
     CDS             2665..3684
                     /gene="ltaE"
                     /locus_tag="VFQ10_06210"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HET9964969.1"
                     /translation="MIDLRSDTVTRPTEGMRRAMLEAPVGDDVFGEDPTVNRLEEYVA
                     DLLGKEAALYAPSGTMTNQVGVQVNTNRGEEVLLHEGSHIFVYEAGAPAMLSSVQLRT
                     LPGEDGVIAPETVCAAVRPEDVHFPRSRLLCLENTHNTSGGKVFPLEDFAAVATTAKE
                     LGLKVHLDGARLFNAQAATGVPASEWCAHADTVSVCSSKGLGAPVGSLLAGDEQTIEG
                     ARRTRKAFGGGMRQAGIIAAASLYAFENHLDRLGEDHEHARNLASGLREAGYEAPEPE
                     TNLVLVAVDDPSEFLQALAREGVLATPGKPGYVRLCTHLDIGEREIEAAIQAAARITS
                     SAIQR"
     gene            3681..4142
                     /locus_tag="VFQ10_06215"
     CDS             3681..4142
                     /locus_tag="VFQ10_06215"
                     /inference="COORDINATES: protein motif:HMM:NF023982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9964970.1"
                     /translation="MRVEATPVPATDLAEPLMLLEEALRDGEPLPEHFADRLRKAVEA
                     GDVEVLAARAEDQMLGVVVLAYRLNVSAGGLFASIEDLHVRPEARRQGVGRALLVAAH
                     ERCTKHNISYVEAQVEDAAAKTFYAALGYEPETGVRVLSRSLAVGHTTTKS"
     gene            complement(4162..4638)
                     /locus_tag="VFQ10_06220"
     CDS             complement(4162..4638)
                     /locus_tag="VFQ10_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9964971.1"
                     /translation="MSRDFTIRPGRREDAAEAARLWVQSAEEHTSHDRVYETAPGAEK
                     TMRRFLADVANSGYSFLFVAAAGDRTVGFISGELRQGSPTFLPKTWASVDDVFVEPAY
                     RNRGIGRALLQSVQAWAQERDADGISLQVAAANSRGRKFYEDLGFRDVSVYKVLEF"
     gene            4722..5471
                     /locus_tag="VFQ10_06225"
     CDS             4722..5471
                     /locus_tag="VFQ10_06225"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HET9964972.1"
                     /translation="MGQKVDRFWEVDAARGIAIIMMIVYHSTYDLDTLGGYDIQSTTG
                     QWALFADVTAGLFLFLVGVSLAISRARTTLTGWSLFRKYLARGLRILVYGVVLTFVFL
                     ALGMGVVAFGILHLIGISIILAYPFLMLRFTNLVLGALIFVAGQYILAQGLDSQSIWL
                     LPFGIVPEGVIMPDYRPLLPWFGVVLIGLFFGNLVYGDGGRPAVTEDEAPLPARPLLS
                     LGRNSLFIYLIHQPIIIALLAATGVVELDFS"
     gene            5851..7686
                     /gene="glmS"
                     /locus_tag="VFQ10_06230"
     CDS             5851..7686
                     /gene="glmS"
                     /locus_tag="VFQ10_06230"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET9964973.1"
                     /translation="MCGIVGYAGRERCVEVLMEGLGHLEYRGYDSAGLALQREGQIER
                     ITEVGQLENLTRALAGRNGDLSQVRTGLGHTRWATHGRPSEENAHPHLGGDGSVAVVH
                     NGIIENFSELKEELEGRGVVFRSETDTEVMAHLIAERVEAGETLRIALAGVFQRLEGS
                     FAVAAISVGEPGKVVAARHQSPLVIGLGEGETFLASAVQALLGETREFLVVENGEIVE
                     IAGTSVEVFTSSGEPVERESFEVDWDAEAVELGGYDHYMLKEIHEQPSALGATLLGRI
                     DSDGVVDLSDVGLDLSEIDRVVVIACGTAYHAGLLGKHAIEKLARIPVEVVIASEYRY
                     ADPIADSRTLVVAISQSGETTDTLAAVQAARSVGARVLAVTNTRGSLITREADAVLLT
                     RAGPEICVASTKTFVAQVAALDLLALALADIRGIIPERELREIGRGLRLAPEKVEETL
                     GLLGDRVEEAVEVFEEARCSLFLGRGISYPVALEGALKLKEISYLPSEGYPAGEMKHG
                     PIALVDEHCPVIAVLGEGLIREKILSNVEETIARGASVIAVAREGDKGAGRMARLVLP
                     VPEAPEILAPFICSVPLQLLAYHVAKARGLDVDKPRNLAKSVTVE"
     gene            7727..8710
                     /locus_tag="VFQ10_06235"
     CDS             7727..8710
                     /locus_tag="VFQ10_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET9964974.1"
                     /translation="MILDVPGEPLRVAEVASPEPGKGQVLLRVHCCAVCRTDLHVVDG
                     ELPDPRLPLIPGHQIVGTVERIGEHTDGFTVGERVGVPWLGWTDGECRYCLSGRENLC
                     ENARFTGYQIDGGYAEYALADGRFCFSIPESFPDLQAAPLLCAGLIGHRSLRFAGDAQ
                     RLGLYGFGASAHIVAQVASHEGRRVFAFTRPGDEEAQEFARELGAEWAGSSEEAPPEE
                     LDAAIIFAPVGALVPAALRAVARGGTVVCAGIHMSDIPSFSYDLLWEERSVRSVANLT
                     RQDGLDFLALAPEVPVRTEVMPFPLEAANEALEALRGGRVRGAAVLVVDAC"
     gene            complement(8753..9637)
                     /locus_tag="VFQ10_06240"
     CDS             complement(8753..9637)
                     /locus_tag="VFQ10_06240"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9964975.1"
                     /translation="MAMDTATDAVIIGNPNSGRAGDEDYLERFAKILRSGGLTVEVLN
                     TEHPNHATELAALAGDRLVVAAGGDGTVNEVLNGLSRQATLGVLPLGTANVLAREFGL
                     PLDPAEACQRILKGERSRVDFGVATNGAGAERRFACMAGIGFDAQVIGAVTPRLKRYL
                     RRLAFQLTAFKVLFGNRFPTLEVSSGDEVHTAKFAIVANGHYYGGDYRVSGPGLLTSG
                     AFETILVERLSDLLRPDVFAGIMARRPLNRAMTSFTSTEVHARAHGAEVPVQLDGELW
                     GGLPMSFHIEPGVLNVVR"
     gene            9684..10082
                     /gene="acpS"
                     /locus_tag="VFQ10_06245"
     CDS             9684..10082
                     /gene="acpS"
                     /locus_tag="VFQ10_06245"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET9964976.1"
                     /translation="MEGFIVPGIGVDVVDVERMQLALERTPKIRERLFTEAEIAYCEK
                     YRFADRHYAGRWAAKEAVTKALGCGLIQWNGVEVVRRPRSAPTVRISGKIKKFADAVG
                     VLEEDLYISITHSELSAVAVCVVRREQISG"
     gene            10079..11584
                     /locus_tag="VFQ10_06250"
     CDS             10079..11584
                     /locus_tag="VFQ10_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET9964977.1"
                     /translation="MKLYTAQEMSRADAGAQDLGIPGGVLMERAGVAMASIALERYSP
                     GRALVVCGGGNNGGDGFVIARELHRSGVDVAVVATKEEYEGDPATNLEILKNLEVRLI
                     AAEDLDAELGRADLVVDALLGTGFSGEVREREAGIIEKMNSAQGPVLAVDVPSGVNGT
                     TGEVQGVAVFADLTVCAHAMKVGCVISPGREHAGEILAMDIGIPHRADVDPSLVWTDA
                     AYLRGKIPRTAEPAHKYSAGALLVVAGSRGTTGAPVMVVKGAQRTGCGIVFLATSQGA
                     ASAVDLALTEALVYGVAEDEQGYMESGALEEILEHSGRASALVMGPGTGTGDEGRRLV
                     EGILREVELPVLLDADAITNLSGKDVLARRDSPTVITPHAGELGRLLGSGAREVSARR
                     LDSARNAAQQHRCCVLLKGSDTLVVDGERVAVNSTGNVALATAGTGDVLSGVIGALLS
                     RGMDTYDAARAGAWTHGRAAELWQEESAWPAESFIATDLLEYLPQAVGELF"
     gene            11494..12375
                     /locus_tag="VFQ10_06255"
     CDS             11494..12375
                     /locus_tag="VFQ10_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET9964978.1"
                     /translation="MARRVCLACGELHCDRPPRISATGGGRVVLTFRLLFIGDIVGPA
                     GCSAVLDLVPRLRRELEPDVVVANAENSAPTGRGITRESGAALLSVADFLTLGNHAFD
                     AEGYEEFLGEEYRVVRPANFGEKDPGLALGVFEAGGVIVGVINVLGRVFVERMRVSPF
                     EAADLAVEELKARGAELLLVDSHAEATSEKQALGHYLDGRAHAVLGTHTHVPTADHSI
                     LPGGTAYVSDVGMTGCKESIIGFEREDFLALFMGDWRGISVATRGPVVLNGALVEFDL
                     DAQRAKGIEAVSREWNP"
     gene            12424..12903
                     /locus_tag="VFQ10_06260"
     CDS             12424..12903
                     /locus_tag="VFQ10_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9964979.1"
                     /translation="MEQQDIRELRVAEISHAEWPTLGPDETVESAIKLFAESGISGAP
                     VVDGGRLVGIVTEGDLIFRDADIKAPGFLDILGGLIPLGSWDEYRKEALKSAGVTVDE
                     VMTRELITISPDATLAEASTIMADKRVKILPVAEGDALRGVITRMDILTLHVLNPHR"
     gene            12912..14027
                     /gene="alr"
                     /locus_tag="VFQ10_06265"
     CDS             12912..14027
                     /gene="alr"
                     /locus_tag="VFQ10_06265"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9964980.1"
                     /translation="MSSTATGVRVPSRTWAEVDPGAVRHNVGTLKRRAPNSRLMAVVK
                     ADAYGHGSVEVARACIGAGADSLAVVTVEEGAELRRAGLEVPILVFTDLPSDRLRLAG
                     KHHLAVTAHSIPSARRIAANPGLEAHLKVNTGMNRWGVDPSEVGEARKILGPRLAGLY
                     THFASADSDEEATRRQIESFESVLGAHPFGGILVHAANSAATLWHPRSHYDCVRPGVA
                     LYGLHPAGDEGDPAAEGLRPAMALKSYVAGVRRLARGDGVSYGLTYHAGEPMFAATVP
                     VGYAEGYRRALSGRAETLIRGQRRPLLGRVTMDACVFGVDEGVEVGDEVVLLGGQGEG
                     RITAEELGSRAGTINYEITTGIDPRRVERSYEDVRGS"
     gene            14011..14364
                     /locus_tag="VFQ10_06270"
     CDS             14011..14364
                     /locus_tag="VFQ10_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964981.1"
                     /translation="MFEDLNWKSAARRSAVVIAIYIGLLYLLSVAFPESGFNMDSRAQ
                     VISLLVNAIIFFFIFTFVYAFVERSRNRRIAEARKQKKPAKQATGEGDQEGDATSLKG
                     RPNPNTSRKKARRRR"
     gene            14368..15189
                     /locus_tag="VFQ10_06275"
     CDS             14368..15189
                     /locus_tag="VFQ10_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9964982.1"
                     /translation="MDLRAALDPPLDAVGLLDVVPELSLARELEGSPYHHLDTLDHVL
                     EVVRGVERELDEGRVGARVRADSAQGLRLAALLHDVAKPVTRGELEGRVLFVSHDSLG
                     AGMVRRIGRRLGLSAWETDLTSTLTALHLKIGFMGNPRTDYPPERLARAAGSFGEELA
                     VLSWADRLAAQGPRLKPEHLRRHEDLCTWFLRVSRELGPYPEPDYAVLEEVLSCASGA
                     DVGYAASYLRLLAARGAAENSDLARLAARRVAVEDPGGGRVESRRERSHSRGRRR"
     gene            15195..15395
                     /locus_tag="VFQ10_06280"
     CDS             15195..15395
                     /locus_tag="VFQ10_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET9964983.1"
                     /translation="MNIKRAKRDRWILGVCGGVAHTYGWSSNVVRLVTVVIAVIIPGP
                     STLLAFVIYLLLGFILPESEEF"
     gene            15400..16032
                     /locus_tag="VFQ10_06285"
     CDS             15400..16032
                     /locus_tag="VFQ10_06285"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET9964984.1"
                     /translation="MDFAEAQKEALGCTKCGLCHSRTQVIFGEGPLNAGLFIVGEAPG
                     FNEDKEGKPFSGASGILLDRMLESLDLGREKVYLTTLVKCRPPGSPPRPPRPSEVSSC
                     RPYLVSQLAAVDPKVVVALGDLPSRVLTGRKESVSRIRGRAIPLDGRYVFPTLSLQRA
                     LYTPADRALLVSDFSRLPELLEAERPSTYETLNVTRSPDAEREPVQPGLW"
     gene            16076..16498
                     /gene="tsaE"
                     /locus_tag="VFQ10_06290"
     CDS             16076..16498
                     /gene="tsaE"
                     /locus_tag="VFQ10_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET9964985.1"
                     /translation="MLEELASRVAGVLGPGDIVVLTGEVGTGKTTFVRAAARSLGVTE
                     RVTSPTYQLARGYEGLKDGRRVRVNHMDLYRLEGIEDHDVLELEDYLEPDAVTFIEWA
                     DPALGVIENPSIVHIYHETPTTRRISLRGPAAERLSPC"
     gene            16492..17157
                     /gene="tsaB"
                     /locus_tag="VFQ10_06295"
     CDS             16492..17157
                     /gene="tsaB"
                     /locus_tag="VFQ10_06295"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET9964986.1"
                     /translation="MLILALDASTPVTTVALARGNGREVLAEVSLTVRGASETLLPAI
                     HAALDLAGEDLGSVGRVLAGVGPGTFTGIRIAAATARAISAGTGIPLSKNSTLDALAA
                     PARLYSGDVLAVLDARRGQVFARRYSEAGPTTDIYCLTPEELSVEGGPLVVGDGAVRY
                     RGALSGLGRIPPEESPLHRVTAVGHVISADLSPVSPDELVPIYVREPDAEVRRDLNPW
                     SHP"
     gene            17154..17606
                     /gene="rimI"
                     /locus_tag="VFQ10_06300"
     CDS             17154..17606
                     /gene="rimI"
                     /locus_tag="VFQ10_06300"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET9964987.1"
                     /translation="MSEAGPHLRRMEARDLPSVMEIDALSLPRPWSAAIWRGELGSPY
                     GLYLVLEERGEVVGQIGIRHVLGELHITTIAVKPEYRRRGHARALIGAALDSYPNASH
                     AHLEVRPTNVEAIALYESLGFRKTGRRPRYYGDEDALLMTLNLDEIHH"
     gene            complement(17572..17835)
                     /locus_tag="VFQ10_06305"
     CDS             complement(17572..17835)
                     /locus_tag="VFQ10_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964988.1"
                     /translation="MRTFSLLFAFIALVMLAVFLIYVFRGPAEPLIAGGIFVSFLACL
                     APALLLLKPFEPPEELRRRREEARREREERRRPSDESHRGSES"
     gene            17874..18938
                     /gene="tsaD"
                     /locus_tag="VFQ10_06310"
     CDS             17874..18938
                     /gene="tsaD"
                     /locus_tag="VFQ10_06310"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET9964989.1"
                     /translation="MATAPRENQAEIVGSFDVPECYKQTMILAIETSCDDTCAAVVEP
                     DGRGALSNVVHTQTEHARYGGIVPEVASRAHLERLDGVVQKSLSDADVSLDEVSRVAV
                     TTRPGLIGALLVGVAAAKGLAYARRFPLVPVNHLEGHVAAGYLTEPGLEPPFVALIAS
                     GGHTALYSVDRDRKLRLLGETLDDAAGEALDKGARMLGLGFPGGPAISKAASNGDRDR
                     YDFPVGLKDKDNLDFSFSGLKTSLLYKLKAMDGERLREELPHLAAGYEAAVVEALSRK
                     LLRAVDLHDAPAVVVAGGVAANTLLRRTIEERCAKIGTGVVVPPPELCTDNAAMIGAA
                     AASAPAVPFPEYLSLNARSL"
     gene            complement(18972..19232)
                     /locus_tag="VFQ10_06315"
     CDS             complement(18972..19232)
                     /locus_tag="VFQ10_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964990.1"
                     /translation="MTILSIFRTDRIPSLSRLPKELGREERTCARCGGRKEHILYRVP
                     KKVVLLYVKDHSENLHATCMVCARSTVLTGDERDRIFASLRD"
     gene            19339..20961
                     /gene="groL"
                     /locus_tag="VFQ10_06320"
     CDS             19339..20961
                     /gene="groL"
                     /locus_tag="VFQ10_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563800.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET9964991.1"
                     /translation="MAHKELRFNTEARRALEAGVNKLADAVRITLGPKGQYVVLDKKF
                     GSPTITNDGVTIAREIELEDIFENQGAQLVKEVATKTNDVAGDGTTTATVLAQIIVHE
                     GLKNVAAGANPVILRSGVDKAVEAAVEAIKAQSTEISGKEEISRVGAISARSPEVGNV
                     IADAIDKVGKDGVVNVEESQTFGMDLEFTEGMQFDKGYLSPYFVTDQDRMEAVLDDPY
                     ILIANQKIGNVQDVLPILNQVMQSSKPLLIVSEDVEGEALATLIVNKLRGTFNAAAVK
                     APGFGDRRKRMLEDIAILTGGEVITEELGLKLENTQLNQLGRARKVVITKDDTTIVDG
                     AGETERITGRINQIRSEIETTDSDFDREKLQERLAKLAGGVAVIKVGAATETELKEKK
                     HRVEDALSATRAALEEGIVPGGGVVFLHAQQPVADLLDSLDGDERTGARIIHRALEEP
                     IRQIATNAGADGSIVVEKVRTEGGNVGFNALSSQYEDLVQAGVTDPTMVSRSALQNAA
                     SIGSLIVTTDVIVAEPEEEAPAMPAGGMGGMM"
     gene            21606..22766
                     /locus_tag="VFQ10_06325"
     CDS             21606..22766
                     /locus_tag="VFQ10_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HET9964992.1"
                     /translation="MDIEIGRGKTARRAYGLDEIAIVPSRRTRDPEDVDISWSLGNLN
                     LDLPCLASALDAAVDPTTAGIIGSLGGLAVLNLEGLQTRYEDPGPVFEEIASLPEHKA
                     TRVMQDIYAEPIKEELIFRRVQEIKDKGVIAAASLTPQRVERYHRAAIEAGLDVLVVQ
                     GTVVSAEHVSRSVEPLNLMEFVPSLNVPVIVGGCASYSTALHLMRTGAVGVLVGVGPG
                     RICTTRGVIGVGVPQATAVADASAARMRHYLETGEYVNVIADGGMRTGGEIAKAITCG
                     ADAVMLGSAFARAEEAPGRGYSWGMATFHPTLPRGTRISTRTTGTLEEILVGPAREND
                     GTLNLMGALKTSMATTGYQNIKEFQKAEVMYAPSLATEGKTEQRSQGVGMGR"
BASE COUNT         4193 a         7101 c         7504 g         4014 t
ORIGIN      
        1 gtcatggtca gggtggggtg ggtggtcttg acgaccaggt ctatcacgct ccggtccgag
       61 gcggtgagat cccggggcga attttctccg agaggcgtga gatctgggac cctgatcaag
      121 gctaccctgt cggctttgag ctcgcgcctt acttcctcgg ctatctgctt gggtggagca
      181 tgggggtcga ggctttttgg cgtcgaggga cggaacacga gctgcgagga gaggatgaag
      241 ttcacgctag tccctgccgc tatgccgagc agattgcaca gcgcgtagta cggaaagtca
      301 tctgagacgt acttcactag cgggtaccag acggcgaagt tcgccccgat gcccatcacg
      361 gcgacgggat aggccaggac gccgcgcagg aagaagtgga tcctgctgga gtgcctgata
      421 tcgcgccagg tgaacgcgtt gttgaggagg aagttgctga ggatggagag cccgaccgcg
      481 aacatccagg ctatgacctt gtgggcgtgg aagtactcgg cgagcacgat gagggtaacc
      541 atgttgacca gtacgccgga ggccccgacg agggcgaact tccagaagcg ccccgccgaa
      601 ggcacgtacc agaacaggct cagtatgtga ctcaggtact cgaagccctg gcgaatggtc
      661 gccttggata ccccagcatg gcgaacctgg aactcgaaag gcgtttcgag taccttgagc
      721 tcgggtgcgc acaccagtat ctcgagcagc accttgaacc ccgtcggcct gaactggatg
      781 cctgagatcg cctggttttt tatgaggaag aagcccgcca tggggtcatt agtcttgcgg
      841 gcttccttga agaggatccg ggccaggtac ttgcttccca cggacacggc ccgccgcatt
      901 ctgccagaga gacctgcgta gccgccctgg ccatagcggc tcgccacaac gacgtcggcg
      961 tcggagcggc gtgcgatctc cagcatctta cgcaccttct cgggagggtg ctgcaagtcc
     1021 gcgtccatca cacaggtgta ctcgctcgcg ccagagaaca gctccatacc cgtcgtcaca
     1081 gccgtcgaga ggccaccgta ccgctctgct ccttcccggc gcaccaggag gatgcggccg
     1141 tcctgttctg caagaccccg tatcacctcc ggcgtttcgt ccgtcgagtc atccacgaag
     1201 accaccctgt aatccaggtc cgaaaggctt tcgcgcaact catgcacgag catcgggaca
     1261 ttctcggcct cgttgcgggt cggcacgacc agggtaagga gtatcttctt ctggctcctc
     1321 ccacgttccg gcccgccggt aaccgcttgc ttctctgctt tcatggctgc tctaggctag
     1381 catattgggt tgacccgccc aaatagggga tagactgacc atagaggctc ccaacggagg
     1441 tacacagaat gcagaacagc gatctacagc gcgcgcacgt ccgggtctca ggccaggtgc
     1501 agggggtctt cttcagggac tcgaccaggc agaaggccga agaactcggc ctggcgggat
     1561 gggtgaagaa cgtgcccgaa gggcaggtcg aagccctctt cgaaggccca tctgataagg
     1621 taagggagat ggtccgttgg tgcgaagaag gcccgcagcg cgcctcggta aagaacgtgg
     1681 acacgaactt cgagagcgcc gagggagatc tcgaaggctt ccaggtccgt tgaacccagg
     1741 caccgaactc cccccagacc tgcgctcgct cctagacaga gaaggtgtcg agatagtagg
     1801 tagcagagcg atagatcacg ggacccagta cgagctggcc cgcgacgacg agacagcgaa
     1861 gctgaacgtc tatcgcacgg gtaagatctc tgccggaggt agagcttcag ggttgcggga
     1921 gctgctcgaa ggctggcgca cgtcgcagac cagcgttggt ggtactaggg cttcgacggc
     1981 ccgcgctgga gggagaccca ttctcgatgg tacgccgagg ctcggcatcg acgaggccgg
     2041 gaagggcgac tacttcggac ctctggtcgt cgccggggtc cggataacac acgcagagac
     2101 ggcgagaaag ctgcaggaga taggggtcag ggattcaaaa gagatcaggg cttcaggcat
     2161 ctcccggatc tcggggcgca tcctggaagc ggtaggccgg aacaacgtcc acgtcatatc
     2221 gctcccgccc ccggagtacg agtccagccg gaccgccgcc ggaaacgtca acaggctact
     2281 cggcgagctc gacgccaaga tcacgggtaa acttgtggac gaggttgaag tagtagttgt
     2341 ggacgagttt gaccgttccg cccgctccta tctcgtgccg ttcgtgcctg acggcattcg
     2401 gctggaggtg cgccctcggg ctgaggacga tgcggcggtg gctgctgcgt cgatagtagc
     2461 gcgggcgcgg caactggagg agatcgaccg tctctcggag cgggtggggt tcggtttgcc
     2521 actcggagcg acgcacgttg tggaggccgg tcggcgggtg gtcgaggaac tcggggaaga
     2581 cggtctggcg gaggtggcga agatccactt cgccaccacg cgtcgggtgc taaagacggc
     2641 cgaacagagt gatggaggcg acccttgata gatcttcgca gcgacacggt gacccgtcct
     2701 acggagggta tgcggcgggc gatgctcgag gcgccggttg gggacgacgt gttcggtgag
     2761 gacccgacgg taaaccggct cgaggagtac gtggctgacc tgctgggcaa ggaggcggcg
     2821 ctttacgcac cttcagggac gatgaccaac caggtcgggg tccaggtgaa cacgaaccgc
     2881 ggagaagagg tgctcctgca cgaaggttcc cacatcttcg tctacgaggc cggagcaccg
     2941 gccatgctct ccagtgtcca gctccgcacc ctccccgggg aggacggcgt catcgccccg
     3001 gagaccgtct gcgccgccgt caggcccgag gacgtccact tcccacgctc caggctgctc
     3061 tgcctcgaga atacccacaa cacctcaggt ggcaaggtct tcccactcga ggacttcgcc
     3121 gcggtcgcca caacggcgaa agagctcggc ctgaaggttc acctcgacgg ggccaggctc
     3181 ttcaacgccc aggctgcgac cggcgtcccc gccagcgagt ggtgcgcgca tgctgacacg
     3241 gtctccgtgt gctcctccaa ggggctagga gcaccagtgg ggtctctgct cgccggggac
     3301 gagcagacca tagagggcgc ccgccggact cgcaaggcct ttgggggtgg tatgaggcag
     3361 gccggcatca tcgccgccgc ctccctctac gccttcgaga accacttgga caggctcggc
     3421 gaggatcacg aacacgcccg caacctcgcc tcaggactga gagaagccgg ctacgaagcc
     3481 cctgaaccgg agaccaacct cgtcctcgtc gccgtcgacg acccttctga attcctgcag
     3541 gcgctggccc gcgagggcgt gctcgccaca cccggcaaac caggctacgt tcgcctctgc
     3601 acgcacctcg acatcgggga aagagagata gaggctgcca tccaggcagc ggccaggatc
     3661 accagcagcg ccatccaacg ttgagggtag aggccacccc tgtccccgca acggacctgg
     3721 ccgagccctt gatgctcctc gaagaagctc tcagggacgg cgagcccttg cccgagcatt
     3781 tcgccgaccg cctgcgaaag gccgtcgagg caggagacgt ggaggtgctg gccgccaggg
     3841 cggaggacca gatgctgggt gtggtcgtcc tcgcctaccg cctcaacgtc tccgcgggcg
     3901 ggctcttcgc cagcatcgaa gacctccacg tgcgcccgga agccagacgc cagggcgtcg
     3961 gccgcgccct gctcgtggcg gcgcatgagc gttgcacaaa gcacaacatc tcttacgttg
     4021 aagcacaggt cgaagacgcc gcagccaaga cgttctacgc cgccctaggc tacgagccgg
     4081 agacaggcgt acgggtcctc tcacgctctc tagctgtcgg ccacacaaca accaagagtt
     4141 gacggctgaa agctgactgc cctagaactc cagcacttta tagacggaga catcacggaa
     4201 ccccagatct tcgtagaact tgcggccccg cgagttggcg gcggcgacct ggagtgagat
     4261 accgtctgcg tctctctcct gcgcccaggc ctggacgctc tggaggaggg cgcggcctat
     4321 accgcggttg cggtaggcgg gttcgacgaa aacatcatct acggaagccc acgttttcgg
     4381 gaggaaagtc ggagagccct gacgcagctc gcccgagata aagcccaccg tccggtcccc
     4441 cgccgcagcc acgaagagaa aagagtaccc actgttcgcc acgtcggcca gaaagcgccg
     4501 catcgtcttc tcggcgccgg gggctgtctc gtagaccctg tcgtgggacg tatgctcctc
     4561 ggcgctctgc acccacagcc ttgcagcctc ggccgcgtcc tccctgcgcc caggcctgat
     4621 ggtgaagtcc ctactcacaa agcaatccta acctatgcgt cgcgggacgc gggtggggta
     4681 tgatccggaa agagcggaga ggagccggga cggagcgcaa ggtggggcaa aaggttgaca
     4741 ggttctggga ggtggatgcg gcccgcggca tcgccatcat catgatgatt gtctaccact
     4801 caacctacga cctggacacc ctcggaggct acgatatcca gtccactacc ggccaatggg
     4861 cgctcttcgc cgacgttaca gcaggcctct ttctgttcct cgtcggcgtc tcgctcgcca
     4921 ttagtcgggc gaggactact ctgacgggat ggagtctctt ccgcaagtac ctggcgaggg
     4981 gcttgaggat cctggtgtac ggagtggtgt tgacctttgt cttcctggcc ctcggtatgg
     5041 gtgtcgtggc gttcggcata ctgcacctga tcggaatctc cataatcctc gcttacccct
     5101 ttctgatgct ccgattcacc aacctcgtgc tcggcgctct catattcgtc gccggccagt
     5161 acatcctggc ccagggcctc gactcacaga gcatctggct cttgcctttc ggcatcgtac
     5221 cagagggtgt gataatgccg gattacaggc ccttgttgcc ctggttcggc gtcgtcctga
     5281 tcgggctgtt cttcgggaac ctggtgtacg gcgatggcgg gcggcccgca gttacggagg
     5341 acgaggcgcc gctaccagcc agaccacttc tctcgctagg caggaactcg ctgtttatct
     5401 acctgatcca ccagcccatc atcattgctc tgctcgccgc aacaggcgtc gtggaactgg
     5461 acttctcgta gagcttcagg gcgatgggtt tcatttttgc accccaaccg ttacagttag
     5521 cggcgtcgtt ccggagaaga tggggaggtg gctcgtagaa gattacattt gcagtagatg
     5581 accagcgagc gcgatgggat cgtctgagct tgcggggcag agagaaacaa gagcgccagg
     5641 actcccgtgg tgcgggagtc gacgaggaag acgtttatcg aagcattcgg cgggtgcgtc
     5701 tcggcttcgg gcggtcgtca gggcggtgac aaatcccggc ggtgacgccg gggacaaaga
     5761 accgttcggg cccggagaca gagcgagagg ctctgtctca tttctctgcc ctccctgatc
     5821 ccatctcctg ataaaaaggg aggtaagtcc gtgtgcggca tagtgggtta cgccggtaga
     5881 gagcggtgtg tcgaggtact catggagggc cttgggcacc tggagtatcg tggttacgat
     5941 tcggcggggc tggcccttca aagggagggc cagatagagc ggataacaga ggtcgggcaa
     6001 ctcgagaacc tcacccgagc ccttgccggg cgcaacggtg acctctccca ggtgcggacc
     6061 gggctgggac acacccgctg ggcgacgcac gggcgaccca gcgaggagaa cgcgcatccc
     6121 catctcggtg gagatggaag tgtcgccgta gtacacaacg gcatcataga aaacttctcg
     6181 gagctcaagg aggagttgga ggggcggggc gtcgtcttcc gttccgagac ggacacagag
     6241 gtgatggcgc acctgatcgc cgagagggtc gaggccgggg agacgttgcg gatagcgctc
     6301 gccggggtgt ttcagcggct cgagggctcc ttcgccgtcg ctgccatcag cgtgggtgag
     6361 cccggtaagg tcgtcgcggc ccgacatcag agcccactcg tcatcggcct cggggaaggt
     6421 gagacctttc tggcgagcgc ggtgcaggcg ctgctcgggg agacgcgcga gttcctcgtc
     6481 gtcgaaaacg gtgagatcgt cgagattgcg ggcacttcgg tagaggtgtt cacctcttcg
     6541 ggcgagcctg tcgagcgtga gtccttcgag gtggactggg acgccgaggc cgtcgaactc
     6601 ggtggctacg accactacat gctcaaggag atccacgagc agccctccgc cctgggagcg
     6661 acccttctcg gccgcatcga ctcagatggc gtcgtcgacc tctccgacgt agggctcgac
     6721 ctctcagaga tagaccgggt tgtggtcatc gcctgtggca cggcctacca tgccggcctg
     6781 ctcggcaaac acgccataga gaagctcgcc agaatccccg tcgaggtagt gatcgccagc
     6841 gagtatcgct acgcggaccc catcgcggat tctcgcacgc tggtcgttgc cataagccag
     6901 agcggagaga cgacagatac cctggcggcc gttcaggcgg cgcggagcgt cggggccaga
     6961 gtgctcgcgg ttaccaatac cagagggtcg ctcatcacac gtgaggccga cgcggtcttg
     7021 ctcaccaggg cggggcccga gatctgtgtt gcctccacca agaccttcgt cgcccaggtc
     7081 gccgccctcg acctgctggc gctggcgctg gccgacattc gcgggatcat ccccgagaga
     7141 gaattgcggg agataggtcg cggcctcagg ctggcgccgg agaaggtgga ggagacgctc
     7201 gggttgctcg gagaccgggt cgaggaggcc gtcgaggtct tcgaggaggc ccgctgctcg
     7261 ctcttcctgg ggcgcggtat ctcgtatccc gtggcgctcg agggggctct gaagctcaag
     7321 gagatctcct acctgccctc cgagggctat ccggcgggtg agatgaagca cggtcccata
     7381 gctctggtgg acgaacactg tcccgttata gccgttctcg gagagggcct catccgcgag
     7441 aagatcctct cgaatgtcga ggagaccatc gccaggggcg ccagcgtcat cgccgtcgcc
     7501 cgtgaggggg acaaaggcgc aggaaggatg gcgcgcctcg tgctccctgt tcccgaggcg
     7561 ccggagattc tggccccctt tatctgtagc gtgcctctgc agctcctcgc ataccacgtc
     7621 gccaaggctc ggggtctcga cgtggacaag ccacgcaacc tcgccaagag cgtcaccgtc
     7681 gagtagggag cgtgcgttct tggagtagga tacgtccgtg cgagcgatga tcctggacgt
     7741 acccggcgag ccgcttaggg tcgcggaggt ggcctcgccc gagccaggca aggggcaggt
     7801 cctcctgcgc gtacactgct gcgctgtgtg caggacggat ctgcacgtcg ttgacgggga
     7861 gttgcccgat cccaggttgc ccctgatccc gggccaccag atagtcggta ccgtagagag
     7921 gatcggcgag cacacagacg gcttcacggt gggagagcgg gtcggtgtgc cgtggctcgg
     7981 gtggaccgac ggcgagtgcc gctattgcct ctccgggcgc gagaacctgt gcgagaacgc
     8041 ccggttcacc gggtaccaga tcgacggcgg ctacgccgag tatgctttgg ccgacggccg
     8101 tttctgcttt tctatccccg agagtttccc cgacctgcag gccgctcccc tgttgtgcgc
     8161 cggcctgatc gggcaccgct cgttgcgctt cgccggcgat gcccagagac tcggtctcta
     8221 cggcttcggg gcctcggctc acatcgtggc ccaggtcgca tcgcacgagg gccggcgcgt
     8281 cttcgccttc accagacctg gagacgagga ggcacaggag tttgccaggg aactgggcgc
     8341 cgaatgggcc gggagttcgg aagaggcgcc acccgaggaa ctggacgcgg cgatcatctt
     8401 cgcgccggtg ggcgccctcg tgcctgctgc cctcagggcc gtcgccaggg gcgggaccgt
     8461 ggtctgcgca ggcatacaca tgagcgacat tccctccttt tcctacgatc tcctctggga
     8521 agagcgatcg gtgcgctcgg tggccaacct cacgcgccag gacggcctgg acttcctcgc
     8581 tctggcccct gaggtgccgg tccgcaccga ggtcatgcct ttccccctgg aggcggccaa
     8641 cgaggcgcta gaagccctgc gcggcggcag ggtgcgtggt gctgccgtgc tcgtcgtcga
     8701 cgcttgctga ccagcgtttg cgaagcggtc ctcgacctat ccgcagacta tcctatcgga
     8761 cgacgttcag gacgcctggt tcgatgtgga aggacatggg cagtccgccc cagagctcgc
     8821 cgtcgagttg tacgggcacc tctgcgccgt gggccctggc gtggacctcc gtcgaggtaa
     8881 aagacgtcat tgcgcggttg agaggcctcc tcgccatgat cccggcgaac acgtcgggcc
     8941 tcaggaggtc gctcaggcgc tcgaccagga tggtctcgaa cgcgccgctg gtcagcaggc
     9001 ctggtcctga caccctgtag tcaccgccgt agtagtggcc attggcgact atggcgaact
     9061 tcgcggtgtg gacctcgtcg ccgctgctta cctccagcgt ggggaacctg ttaccgaaga
     9121 gcactttgaa cgccgtcaac tggaaggcca ggcgccgcag gtagcgcttc aggcgcggtg
     9181 tcacggcgcc tatcacttgc gcgtcgaagc ctatccccgc catacaggcg aagcggcgtt
     9241 cggcgccagc accgtttgta gcgaccccga agtccaccct ggagcgttcc cctttcagta
     9301 tgcgctggca ggcctcggcc gggtcgagcg gcagcccgaa ttcccgcgcc agcacgttcg
     9361 ccgtgccgag cggcagcacg ccgagcgtgg cctgccgcga gagcccgttc agtacttcgt
     9421 tgaccgtgcc gtcgcccccg gcggccacga ccagccggtc ccctgcaaga gcggcgagct
     9481 cggtggcgtg gttggggtgt tcggtgttga gtacctccac cgtcaggcct cccgagcgca
     9541 ggatcttcgc gaaccgctcg agataatcct catcgcccgc tcgtcccgag ttcgggttac
     9601 cgatgatgac cgcgtctgtg gccgtatcca tcgccatact atagcccttt tgccggggct
     9661 gaatctgccg taacatacgc cccgtggaag gtttcatcgt accagggatc ggggtcgacg
     9721 tcgtcgacgt cgagaggatg cagttggccc tggagcgcac cccgaagata cgagagcgcc
     9781 tgtttaccga ggccgagatc gcctactgtg agaagtaccg ctttgccgac cgccactacg
     9841 ccggacgttg ggccgccaaa gaagccgtta caaaggctct ggggtgcggc ctgatccagt
     9901 ggaacggcgt cgaggtggta cggaggcccc gtagcgcacc aacggttcgt atatccggca
     9961 agatcaagaa gttcgccgac gcggtcggcg tgctcgagga agacctctac atctccataa
    10021 cccactccga actctccgca gtcgcagtct gcgtcgtgcg ccgggaacag atctcgggat
    10081 gaagctctac accgcacagg agatgtcccg ggcagatgcg ggggcgcagg atctcggcat
    10141 ccccggcggc gtgctgatgg aacgcgccgg cgttgccatg gccagtatcg ccctggagag
    10201 atattccccc gggcgggcgc ttgtcgtctg cggcggcggc aacaacggtg gggacggttt
    10261 cgtcatcgcc cgcgagctac accgctccgg cgtcgacgtt gcggttgtgg cgacgaagga
    10321 agagtacgag ggagacccag cgaccaacct ggagatactc aagaacctgg aggttcggct
    10381 catcgccgca gaagaccttg acgcggagct cggccgggcc gaccttgtcg tcgacgcgct
    10441 tctcgggaca gggttctcgg gcgaggtaag ggagagggag gccgggatca tcgagaagat
    10501 gaactccgct caaggtcccg tactcgccgt cgacgtgccc tccggtgtga acgggacgac
    10561 gggtgaggta cagggcgttg ccgtattcgc cgatcttacg gtctgcgcgc acgcgatgaa
    10621 ggttgggtgt gtgatctcac ccggccgcga gcacgccggc gagatcctcg ccatggatat
    10681 aggtatccca cacagagcag acgtcgaccc ctctctggta tggacggacg cggcctatct
    10741 acgaggaaag ataccgcgta ccgctgagcc tgcccacaag tactcggccg gggccttgct
    10801 cgtcgtcgcc ggctcccgtg gcaccacagg agcccccgtt atggtcgtca agggcgcgca
    10861 gagaacaggg tgcggcatag tcttcctcgc cacctcccag ggagcggcat ccgccgtcga
    10921 cctcgccctc accgaagcgc tcgtctacgg cgtcgcggag gacgaacaag gctacatgga
    10981 gtcaggggcg ctcgaggaga tcctggagca ctcagggagg gcctctgcgc tcgtgatggg
    11041 cccggggacg gggacagggg acgagggcag gcggctcgtc gaaggtatcc tgcgcgaggt
    11101 cgagctgccc gtgttgctcg acgccgacgc catcacgaac ctctcgggca aggacgttct
    11161 tgcgcggagg gactccccga ccgtcataac accgcacgca ggagagttgg ggcgtctgct
    11221 tggatctggg gcgagagagg tgtccgcccg caggctcgat tcagcccgca acgcggccca
    11281 acagcaccgt tgctgcgtat tactcaaggg ttcagatacg ctcgtcgtcg atggtgaaag
    11341 agtcgccgtc aattctacgg ggaatgtcgc gcttgccacg gcggggacag gggacgtgtt
    11401 gtccggtgtg ataggggctc tactctcgcg cgggatggac acctacgacg cggccagggc
    11461 cggagcctgg acgcacggac gcgcggccga gctgtggcaa gaagagtctg cctggcctgc
    11521 ggagagcttc attgcgaccg acctcctcga atatctgcca caggcggtgg gagagttgtt
    11581 ctgacgttca ggcttctctt catcggggac atcgtcgggc ccgcaggatg ctcggcggtc
    11641 ctggacctcg taccgcggtt acgtcgggag ctggagcccg acgtcgtcgt cgccaacgcc
    11701 gaaaactcgg ccccgacggg tcgtggtatc acccgcgaga gcggcgccgc cctgctctcc
    11761 gtggccgact tcctcaccct aggaaaccac gcattcgacg ccgaaggcta cgaggagttc
    11821 ctcggggagg agtacagggt ggtgcgtccc gccaacttcg gcgaaaagga ccctgggcta
    11881 gccctgggtg ttttcgaggc tggcggcgtg atcgtgggcg tcatcaacgt gctcgggcgc
    11941 gtattcgtcg agaggatgag agtatcgccc ttcgaggctg ccgacctggc cgtcgaggag
    12001 ttgaaggccc gcggcgccga gctcctgctc gtcgattccc acgccgaggc cacgagcgag
    12061 aagcaggcgc taggccatta tctcgacggg agggcgcacg ccgtattggg cacccatacg
    12121 cacgtgccca cggctgacca ttcgatcctg cctggtggaa ctgcatacgt gagtgacgtg
    12181 ggcatgaccg gttgtaaaga gagcattatc ggcttcgagc gggaggactt cctcgctctg
    12241 ttcatgggtg attggcgtgg gatctcagtc gccacccgcg gtccagtcgt gctcaacggc
    12301 gcgctcgtcg agttcgacct cgatgctcag cgggccaaag ggatcgaagc cgtaagcagg
    12361 gagtggaacc cttagccgcc gggccgttta caatggcggc aacgacgatt agtggggtgg
    12421 aacatggaac agcaggacat cagggaattg agggtggcag agatctcgca cgcggagtgg
    12481 cccacgctcg ggcctgacga gacggtggag agtgcgatca agctcttcgc cgagtctggt
    12541 atctcgggcg ctcctgtcgt cgacggcggg aggctcgtcg gcatagtcac ggagggagat
    12601 ctgatcttcc gtgacgctga catcaaggca ccaggtttcc tcgacatcct aggcggcctc
    12661 atcccacttg ggagttggga cgagtaccgc aaagaagcac tcaagagcgc cggcgttacc
    12721 gtcgatgagg tgatgacgag ggagctgatc accatctccc ccgacgccac tctcgcggag
    12781 gcctccacca tcatggccga caagagagtc aagatcctac ccgtcgccga aggtgacgcc
    12841 ctccgcggtg tgatcacccg catggatatc ctgaccctcc acgtacttaa cccccaccgg
    12901 taagtacgac cgtgagcagc acggccaccg gagtcagggt cccgagccgt acctgggctg
    12961 aggtggatcc tggcgccgtg cgccacaacg tgggcacact caaacgcagg gccccgaact
    13021 cgcgcttgat ggccgtggtc aaggcggacg cttacggcca cggctctgtg gaagtggccc
    13081 gcgcctgtat cggggccggg gcagattctc tcgccgtcgt caccgtcgag gaaggggcag
    13141 agctgcggcg cgcgggtctt gaagtgccga tcctggtctt caccgacctc ccgtcggaca
    13201 ggctgcggct ggccgggaaa catcacctgg ccgtgacggc ccactccatc ccgagcgcga
    13261 ggcgcatagc ggccaaccca ggtctcgagg cgcacctgaa ggtgaacacg gggatgaacc
    13321 gctggggagt ggatccctcg gaggtggggg aggcgcgcaa gatacttggc ccgaggctcg
    13381 ccggcctcta cacgcatttc gcctcggccg attccgacga ggaggcgacg agacgccaga
    13441 tcgagagctt cgagtccgtt ctcggcgcgc accccttcgg gggtatcctg gtgcacgccg
    13501 caaactctgc ggccaccctc tggcatcccc gctcgcacta cgattgcgtc cgtcccggcg
    13561 ttgcgctcta cggtttgcac cctgctggcg atgagggaga cccggcggcg gagggtctac
    13621 ggcccgcgat ggcactcaag agctacgtcg ccggcgtgcg gcgactcgcc aggggggacg
    13681 gggtatccta cggattgacc taccatgccg gggagccgat gttcgcggcg accgtgcctg
    13741 tagggtacgc ggagggatat cggagggcgc tctcgggaag ggctgagacc ctgatccggg
    13801 gccagaggcg cccgctcctc gggcgggtca ccatggatgc ttgtgtcttc ggggtcgatg
    13861 aaggtgtcga ggtcggggac gaggtcgtgt tgctcggcgg ccagggtgaa gggcgcatta
    13921 cagccgaaga gctcggctcc cgcgcaggca cgataaacta cgagatcacc acggggatag
    13981 acccccgccg cgtcgagagg agctacgagg atgttcgagg atcttaactg gaagtcagcc
    14041 gccaggaggt cggcggtagt catagcgatc tacatcggcc tgctctacct cctcagcgtg
    14101 gctttccccg aaagtggctt caacatggac tcccgggccc aggtcatcag cctgctcgtg
    14161 aacgccatca tattcttctt catcttcacg ttcgtctacg cattcgtgga gcgcagtaga
    14221 aacaggcgca tagccgaggc tcgtaagcag aagaagccag caaagcaggc cacaggcgag
    14281 ggcgaccagg agggcgacgc cacttcgctc aaaggtcgtc ccaatcccaa tacgagccgc
    14341 aagaaggccc gacgccgacg ctaacccttg gacctccgcg cggccctgga ccccccgctg
    14401 gacgctgtcg ggctcctcga cgtcgtcccc gagctctccc tcgcccgtga actggaagga
    14461 agcccgtacc accacctgga caccctggat cacgtcctcg aggtagtccg gggcgtcgag
    14521 cgcgagctgg atgagggcag ggttggcgcc cgcgtgaggg cagatagcgc acagggtctg
    14581 cgcctcgctg ccctgctgca cgacgtggcc aagcccgtta cccgcggcga gctcgaaggc
    14641 agggtcctct tcgtctccca cgattctctt ggcgcgggga tggtccggcg catcggccgc
    14701 aggcttggcc tatcggcctg ggagacggat ctgacgtcga ccctgaccgc gctgcatctg
    14761 aagataggct ttatgggcaa tccccgtacc gactaccctc cggagaggct cgcaagggcc
    14821 gccggatctt tcggtgagga actcgctgtg ctgagctggg ccgaccgcct cgccgcccag
    14881 ggtccgcgcc tcaagcccga gcacctgcgg cgccacgagg atctctgcac ctggtttctg
    14941 cgcgttagcc gcgagctggg cccgtacccc gaacctgact acgcggtgct ggaggaagta
    15001 ctctcctgtg cgtcgggcgc cgacgtcgga tacgcggcga gctatctcag gcttctcgcg
    15061 gcccgtgggg cggcagagaa tagcgatctg gcgcgactcg ctgctcgtcg cgttgctgtg
    15121 gaagaccctg gcggcggtcg tgtagaatcg cgtcgagagc gtagccactc gcggggccgg
    15181 aggaggtagg ggttttgaac atcaagaggg cgaagaggga tcgttggatt ctgggtgtct
    15241 gcggcggcgt ggcccacact tacggttgga gctccaatgt agtacggctc gttacggtgg
    15301 tgatcgccgt gatcatcccc ggtccgagca cgctgctggc tttcgtgatc tacctgttgc
    15361 tgggcttcat cctccctgag agcgaggagt tctaggcgct tggactttgc ggaggcccag
    15421 aaggaggccc ttggctgcac gaagtgcggg ctgtgccatt ccaggacaca ggtcatcttc
    15481 ggcgaggggc cgctgaacgc cgggcttttc atcgtcggtg aggcacctgg cttcaacgag
    15541 gacaaggagg gcaaaccctt ttcaggagcc tcggggatcc tcctcgaccg catgctcgag
    15601 tctctcgacc tcgggcgcga gaaggtctac ctgaccacgc ttgtcaagtg ccgcccgcca
    15661 ggttcgccgc cacggccacc gaggccttct gaggtaagtt cctgccgacc ttacctcgtt
    15721 tcccaactcg cggccgtcga ccccaaggtc gttgttgcac tcggggatct gcccagccgg
    15781 gtactcaccg ggaggaaaga gtccgtctcg cgcatcaggg ggcgtgcgat cccgctcgac
    15841 ggccgctacg tctttccgac gctctccctc cagcgcgccc tctacacgcc ggcagaccgg
    15901 gcgctcttag tctcggattt ctcccgcctg cccgagctcc tcgaggccga acggccctcc
    15961 acctacgaga ccctcaacgt cacccgctcc cccgacgcgg agcgggaacc agtccagccg
    16021 gggctctggt agagtcccag ggttccccgt ggagctcgaa ggcctgaagg aagctatgct
    16081 cgaggagctg gcatccaggg tagcaggggt gcttggtcct ggtgacatcg tcgtgctcac
    16141 cggcgaggtc ggcaccggca agaccacctt cgtgcgggcc gcggcccgct ctcttggcgt
    16201 aacggagagg gtgacgagcc cgacctacca gctcgcccgc ggctacgagg ggctcaagga
    16261 cgggcgacgg gttagggtca accacatgga cctctatcgc ctggagggga tcgaagacca
    16321 tgacgtactg gagctcgagg actacctgga acctgatgcc gtaacgttta tagagtgggc
    16381 agatcctgca ctcggtgtta tcgagaaccc ttccatcgtc catatctacc acgagacccc
    16441 gaccacgcgg cgcatcagcc tcagaggacc ggccgcagag cgtctgagcc cttgctgatc
    16501 ctggccctcg acgcctcgac tccagtaacc accgtggccc tcgcccgcgg gaacggccgc
    16561 gaggtgctcg ccgaggtctc cctcaccgtc cgcggggcct ctgaaacact gcttcccgcc
    16621 atacacgccg ccctcgacct cgccggggag gatctcggtt cagtggggcg cgtgctcgcc
    16681 ggggttggac cggggacctt tacgggcata cgcatagcag cggcgacggc ccgcgctatc
    16741 tccgcgggta ccggcatccc gctgtcgaag aactcgaccc tggacgccct cgccgccccc
    16801 gcgcgcttgt attctgggga cgtgctcgca gtactcgatg ccaggagagg gcaggtcttc
    16861 gcccgcaggt attcggaggc tgggcctacg acggacatct actgcctcac gcctgaggag
    16921 ttgtcggtgg agggaggacc tttggtagtc ggcgacggtg ccgtacgcta tcgtggcgcg
    16981 ctctctggcc ttgggcgcat accgcctgag gagtccccgc tccacagggt cacggcggtc
    17041 gggcacgtta tctctgccga cctctccccc gtgagccccg acgagctcgt ccccatctac
    17101 gtccgcgaac ccgacgccga ggttcgtcgc gacctcaacc cttggagcca tccttgagcg
    17161 aggctggacc gcacctgcgc cggatggagg ccagggatct cccctccgtt atggagatag
    17221 acgctttgtc cctcccccgt ccctggagcg cggcgatctg gcgcggagag ctcgggagtc
    17281 cctacggact ctacctggtg ctcgaagaaa gaggcgaggt cgttggccag atcgggatcc
    17341 gccacgtcct cggcgagctg cacataacca ccatcgccgt aaagcccgag tatcgcaggc
    17401 gtggacacgc ccgcgccctg atcggcgccg cgctcgactc ctatcccaat gcaagccacg
    17461 cccacctcga ggtacgcccc accaacgtgg aggccattgc gctctacgag tcgctcggct
    17521 tcagaaagac gggacgcagg cctcgctact acggagacga ggacgcattg ctcatgactc
    17581 tgaacctcga tgagattcat cactaggacg ccttcgctcc tcgcgttccc tgcgggcctc
    17641 ctccctgcgg cgccgtagct cctcaggggg ctcgaaaggc ttcagcagca acagcgcagg
    17701 ggcgaggcag gccagaaacg agacgaagat gccgcccgcg atcaggggct cggcagggcc
    17761 cctgaacacg tagatgagaa acaccgccag catcaccagc gctataaagg cgaaaaggag
    17821 tgagaacgtc cgcatggcct caagtatatc cggtcggccc gccggccccg ttggtggcga
    17881 ccgcgccccg cgagaatcag gcggagatcg tcggttcctt cgatgtgccg gagtgctata
    17941 aacagaccat gatcctggcc atcgaaacct cctgtgacga cacgtgcgcc gcggtcgtgg
    18001 aacccgacgg ccgcggggcc ctctcgaacg tcgtccacac gcaaacagag cacgcccgct
    18061 acggggggat cgtccccgag gttgcctccc gcgcccatct cgaacgcctc gatggggtag
    18121 tgcaaaagtc actgtctgac gctgatgtct ctctcgacga agtgagccgc gtggcggtca
    18181 cgacgcggcc tggtttgatc ggggccctcc tcgtcggggt tgctgccgcg aagggacttg
    18241 cctacgcccg caggtttcct ctggtccccg tgaaccacct ggaaggccac gtcgccgccg
    18301 ggtacctcac agagcccgga ctggagccac cattcgtcgc cctgatcgcc tcaggcgggc
    18361 atacggccct ctactctgtg gaccgggatc gcaagttgcg cctgctcgga gagacgctcg
    18421 acgacgccgc cggcgaggca ctggacaagg gggccaggat gctgggtctc ggtttccctg
    18481 gcgggcctgc gatctctaaa gccgccagca acggagaccg tgaccgctac gacttccccg
    18541 tcggcctgaa ggacaaggac aacctagact tcagcttctc cggcctcaag acgagcctgc
    18601 tttacaagct aaaggcaatg gacggagagc gactccgcga ggagttgccg cacctggcgg
    18661 ccggctatga ggctgcggtc gtagaggccc tctcccgcaa gctcctacgg gccgtggatc
    18721 tccacgacgc gcccgccgta gtcgtggcag gcggcgtcgc cgccaacacc ctcctacgcc
    18781 gtaccatcga agaacgatgc gcgaagatag gaaccggcgt cgtcgtccct ccaccggaac
    18841 tgtgtacgga taacgcagcc atgatcgggg ccgccgccgc gagcgctccc gccgtcccct
    18901 tccccgaata cctctccctc aacgcccgca gcttgtagag gcggttcgcg aaccgccctt
    18961 ggaacaatac cctagtcgcg cagcgaggcg aagattcggt cgcgttcgtc gccggtgaga
    19021 acggtgctcc tagcgcatac catgcaagtc gcgtgcagat tctccgaatg gtctttgacg
    19081 tagagcagaa caaccttctt agggaccctg tagaggatgt gctccttgcg cccgccgcag
    19141 cgcgcgcatg ttcgttcttc acggcccaac tcttttggca ggcggctcag gctcggtatc
    19201 ctgtcggtgc gaaagatact taggatggtc atgtttgaat tgtaaccccg ccaggcatat
    19261 actctccaca ttggcactct aatcgagaga gtgccaggca gttgaaatcg cgtcctagtt
    19321 taacagagga ggcagacagt ggcgcacaaa gagctaaggt tcaataccga ggcacgtcgg
    19381 gcgttggagg cgggcgtaaa caagctcgct gacgcggtca ggatcacgct aggtcccaag
    19441 ggccagtacg tggtgctcga caagaagttc ggttcgccga cgatcacgaa cgatggcgtg
    19501 acgatcgcgc gtgagatcga gctagaggac atctttgaga accagggagc ccagctcgtc
    19561 aaggaggtcg cgaccaagac caacgacgtg gctggtgacg ggaccaccac ggcaactgtt
    19621 ttggcccaga tcatcgttca cgagggtctc aagaacgtgg ctgctggggc caaccctgtc
    19681 attttgcgca gtggtgtcga caaggctgtg gaggcggcgg tcgaggcgat caaggcccag
    19741 tccaccgaga tcagcggcaa ggaagagatc tcgcgcgtag gtgccatctc cgcgcgttct
    19801 cccgaggtcg gcaacgtcat cgctgatgcc attgacaagg taggcaagga tggggttgta
    19861 aacgtcgagg agtcccagac cttcgggatg gatctggagt tcaccgaagg gatgcagttc
    19921 gacaagggtt acctgagccc gtacttcgtc accgaccaag accgcatgga ggcggtcctc
    19981 gacgatccct acatcctgat cgccaaccag aagatcggca acgtccagga cgtgctgccc
    20041 atcctaaacc aggtgatgca gtccagcaag ccgctcctca tcgtctccga ggacgtcgag
    20101 ggtgaggctc tggctacgtt gatcgtcaac aagctgcgcg gcacgttcaa cgcggctgcg
    20161 gtcaaggccc ctggcttcgg tgacaggcgc aagaggatgc tggaggacat agccatcctt
    20221 acaggcgggg aggtaataac cgaggagctc ggcctgaagc ttgagaacac ccagctcaac
    20281 cagctggggc gtgccaggaa ggtggtaatc accaaggacg acaccaccat cgtcgacggt
    20341 gccggcgaga ccgagaggat cacgggccgc atcaaccaga tccgctccga gatcgagacc
    20401 accgactcgg acttcgaccg ggagaagctc caggagcgtc tcgccaagct ggccggtggc
    20461 gtggccgtca tcaaggtcgg cgctgctacc gagacggagc tcaaggagaa gaagcatcgc
    20521 gtggaggacg cactctctgc gacccgcgcg gccctcgagg agggcattgt gccgggcggt
    20581 ggcgtcgtgt tcttgcacgc acagcagccc gtcgccgacc tgctcgactc cctcgacggt
    20641 gacgagcgga cgggcgccag gatcatccac agggcgcttg aggagcccat tcgccagatc
    20701 gccacgaacg caggggcaga cggctccatc gtggtggaga aggtgcgcac cgagggcggc
    20761 aacgtcggct tcaacgccct gagcagccag tacgaggacc tagtgcaagc aggtgtgacc
    20821 gacccgacga tggtttcgcg ctcggcgttg cagaatgcgg cttccatagg cagcctgatc
    20881 gtgaccacgg atgtgattgt ggccgagccc gaggaggagg cgcccgctat gccagcaggt
    20941 ggcatgggtg gcatgatgta aggagctctc cagaagagct tctacccgcg gcgtcccgaa
    21001 aggggcgccg ctttttcgta accaatcctg tttcttctgg gtctgaaaag gtatatattg
    21061 cggcgtagcc ggccacagag agatagggag cgaggtgagg gaggatagat aggagtctcg
    21121 ccatagtggt cgtcgtcttg gtggtgatac ttctggccgt gctcgtgttg ccgttcgtta
    21181 cttaggcaaa ggtctctggc gagggaggtg agaaagtgcg gggtgggctc ataccaatac
    21241 ttgtagtcat tcttttgata ctcttgatct tgctgctgct ctcgcggctc ctctagagag
    21301 gctccgccga agaggcagct ctttctggcg caggggatgg gtctccaggg aggtggaaca
    21361 gtgagtaaca gcaacctgct taccatcgca gtggtgctca tcatcgtagt agtgatactc
    21421 ggccttctgg ggataatacc gatattctag gtctggtccg ctgcttgacc ctagcggcgg
    21481 tcgcggtgta gcataagctc tattgctacg tccggaagaa aagtgtatcg gggcccacgg
    21541 aggcagttct ccgtgggctt cgttctatct gtccgcaacg accggaaagg aagctcggca
    21601 cccacatgga tatcgagata gggagaggca agaccgcgcg ccgggcctac gggctcgatg
    21661 agatcgccat agtaccgagc cgaaggactc gcgaccccga ggacgtagat atctcctggt
    21721 cgctcggcaa cctcaacctc gacctgccct gcctggcaag cgctctcgac gcggccgtcg
    21781 atcccacgac cgcaggaata atcgggtctc tggggggcct cgccgtcctc aaccttgagg
    21841 gactgcagac gaggtacgag gaccctggtc ccgtcttcga ggagatagcg agcctgcccg
    21901 agcacaaggc cacgagggta atgcaggata tttacgccga gccgatcaaa gaggagctca
    21961 tcttccgccg cgtgcaagag ataaaggaca agggcgtcat agcggcggcc tcgctcacgc
    22021 cgcagcgggt cgagcgttac cacagggcgg ccatagaggc cggcctcgac gtcctcgtcg
    22081 tccaggggac cgtggtctcc gccgagcacg tctccaggtc cgtcgaacct ctgaacttga
    22141 tggagttcgt gccttccctg aacgttcctg taatagtcgg tggctgcgcg agctactcga
    22201 ctgcgctcca cctcatgcgg actggggctg tcggcgtcct cgtcggtgtc ggaccaggac
    22261 gcatctgtac gacgcggggt gtgatcgggg tcggggtgcc gcaggctacg gccgtggccg
    22321 acgcctcagc cgcacgtatg cgccactacc tggagaccgg tgagtacgtg aacgtcatcg
    22381 ccgacggcgg gatgcgcacg ggcggtgaga tcgccaaggc gataacttgc ggggccgacg
    22441 ccgtgatgct gggcagcgcc ttcgccaggg cggaggaggc ccctggccgc ggctactcgt
    22501 gggggatggc taccttccac ccgaccctcc cgcgcggcac ccgcatcagc acgaggacta
    22561 cggggacgct cgaggagata ctcgttgggc cggcgcggga gaacgacggg acgctcaacc
    22621 tcatgggcgc cctgaagacg agcatggcga cgaccggcta ccagaatata aaggaattcc
    22681 agaaggctga ggtcatgtac gcgccctcgc tggcgaccga aggcaagacg gagcagcgct
    22741 cccagggcgt ggggatgggc cgttagaacg gcttggtcgg caagaatgac atcgagttca
    22801 tcgaggctga tg
//
LOCUS       DASTKM010000151         4395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1448015, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000151 DASTKM010000000
VERSION     DASTKM010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448015"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..>4395)
                     /locus_tag="VFQ10_06330"
     CDS             complement(143..>4395)
                     /locus_tag="VFQ10_06330"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HET9964993.1"
                     /translation="EESPPDFEPEDLAETRAFLEWIDDDNFTFLGYREYDLISQDGEE
                     ALCAVQGSGLGILRQTESGPVSRSFAELPPGVRRLARTPKLLNLTKANSRATVHRPSY
                     LDYIGIKKFDESGAVTGERRFLGLYTFSAYSASAFDIPLVRRKVRYVLERGGFPEGSH
                     NEKDLIEILETYPRDELFQVSKEELFEIAMGILHLQERQRVRFFVRRDTYGRFFSCLV
                     FVPRDRYNTLIRERMQDILLKAFDGVNVEYNVRLSESVLARVHFTIYTKPGEKPEYDE
                     EEIEGRLVETTRSWTDNLYDALIEQYGEERGTELFRKYRDAFSPGYRAGFLPRTAVSD
                     IQRMERLKSNDDLEMSLYHPIEEPEDFLGFKLFKLGEQVSLSEILPLLEDMGVEVVDE
                     RPHEVKPEGSPPVWIYDFGLVHETDDELQTGEVREIFQDAFARAWRGTVENDGFNRLV
                     LRARLTWREISMLRAYCKYLRQTGTTFSQDYMEDALVNNQHIARLIVDLFEARLDPSR
                     RDRTESERLEEEIEEALEAVVSLDEDRILRSFLNITLATLRTNYYQSTPDGDPKPHIS
                     FKLDPEKIPGMPKPLPRFEIFVYSPRTEGVHLRGGEVARGGIRWSDRREDFRTEILGL
                     MKAQTVKNAVIVPVGAKGGFVVKRPPAEGGREALQQEVVACYKTLIRGMLDITDNISG
                     DDIVPPPDVTRYDDDDPYLVVAADKGTATFSDIANELSAEYGFWLGDAFASGGSVGYD
                     HKEMGITARGAWESVSRHFRELGKNIQTEDFTVVGIGDMSGDVFGNGMLLSRHIKLVG
                     AFNHMHIFLDPDPDPEKSFEERERLFRLPRSSWTDYDESIISEGGGIFPRTAKSIPLS
                     PQVREMLDVEAESMTPTELINAMLKAELDLLWNGGIGTYVKAGSESNAEVGDRANDAL
                     RANGDELRCRVVGEGGNLGFTQRGRIEYALCGGRIYMDAIDNSAGVDCSDHEVNIKIL
                     LDAIVEAGDMTEKQRNELLASMTEEVGDFVLRDNYQQTQAISQALALAYPMVDVHARY
                     IHTLEHEGRLDRALEFLPDDEELGERRSENKGLTAPELAILLSYSKITNYQDLLNSDA
                     PEDPYLSRELERYFPTPLRERFREQIHEHRLHRQITATHVTNSLVNRNGPSFVFRLGE
                     ETGAAAPDIARAYMAAREIFDLRTLWDGVEALDNEVEAWIQTRMMLDARKLVDRATRW
                     LLRYRRPPLNIGATISHFSEGAAELSERIPGILLDGDREAVANAAQRLIDANVPPDLA
                     QRAANLGPMFSALDITDVANSTEESLDTTAAVYFILGDRLKLHWLRRHIEALPRDNRW
                     RTLARSALRDDIFNQQAALTAEVLSETPKDNPADERIEEWVEASKGPVDRTLQVLADI
                     NASGTFDLSTLSVALREIRNLITTPESPPEEVEATART"
BASE COUNT          715 a         1345 c         1439 g          896 t
ORIGIN      
        1 tcccgcccca cagcctcatc gtcggcattc ccgctaaacg cgccggcgaa gtgagcgagg
       61 agcaggcagc ggacatagcc cgcggcgcga gcgagtacat agagcgcgcc aaagagcacc
      121 gggagtcgtt gggaggcggg gactaggttc gggcggtggc ttccacctcc tctggtgggg
      181 attccggcgt ggttatgagg ttccggatct cccgcagcgc taccgagagg gtggagaggt
      241 cgaaggtgcc gctggcgttt atgtcggcga ggacctgcag ggtgcgatcc actggtccct
      301 tactggcttc gacccattct tctatgcgct cgtcggcggg attgtctttc ggagtttcgc
      361 tgagcacttc cgcggtgagg gcggcctgct ggttgaagat gtcgtcgcgc agggccgagc
      421 gtgcgagggt gcgccagcgg ttgtcgcggg ggagcgcctc tatgtggcgg cgtagccagt
      481 ggagcttcaa cctgtcaccc aggatgaagt agacggccgc ggttgtatcc agagactctt
      541 ccgtggagtt ggccacgtcg gtaatatcaa gagcggagaa catcggaccc aggttcgcag
      601 cccgctgtgc cagatccggc ggcacgttag cgtctatcag gcgctgggcg gcgttcgcca
      661 ccgcttcgcg gtcgccgtcg agcaggatcc cggggatgcg ttcggagagc tccgcagccc
      721 cttcggagaa atgcgagatc gtcgccccta tattcagcgg cggacggcgg tagcggagca
      781 accagcgggt ggcgcgatcg acgagcttgc gggcgtcgag catcatcctg gtctgtatcc
      841 acgcctcgac ctcattgtcc agggcttcga cgccgtccca cagggtgcgc aggtcgaaga
      901 tctcacgcgc cgccatgtaa gcgcgcgcga tgtcgggcgc cgcggctccc gtctcctcac
      961 ccagcctgaa cacgaaggat gggccgttac ggttcaccag gctgttcgtg acgtgggtgg
     1021 ccgtgatctg ccggtgcagc ctgtgctcgt ggatctgttc cctgaaccgc tctctcaagg
     1081 gtgtcgggaa atacctctcc aactcgcgcg agagataggg gtcctcgggg gcgtccgagt
     1141 tcaggaggtc ctggtaatta gtgatcttgc tgtacgacaa cagtatggcg agctcggggg
     1201 cggtgaggcc cttgttctcg gagcgtcgct cgccgagctc ctcgtcgtcg ggcaagaact
     1261 cgagcgcgcg gtcgagcctg ccctcatgct ccagggtgtg gatgtagcgg gcgtgcacgt
     1321 cgaccatggg gtacgctagg gcgagggcct ggcttatggc ctgcgtctgc tggtagttat
     1381 cgcgcaggac gaagtcccca acctcctccg tcatgctggc gagcagctcg ttgcgctgct
     1441 tctctgtcat gtcaccggcc tcgacgatgg cgtcgagcag gatcttgatg ttcacctcgt
     1501 ggtccgagca gtccacccct gccgagttgt cgatggcgtc catgtagatc ctgccgccac
     1561 agagcgcgta ctcgatgcgc cccctctgcg tgaacccgag gttcccgccc tccccgacca
     1621 cgcggcacct gagctcgtcg ccgtttgccc tgagggcgtc gttcgccctg tcgcccactt
     1681 ctgcattgct ctccgagcca gccttgacgt aggtgccgat gccgccgttc cagagcagat
     1741 ccaactcggc tttgagcatg gcgtttatga gctcggtcgg cgtcatggac tcagcctcga
     1801 cgtcgagcat ctcacgcacc tgcggcgaga gcgggattga cttggccgtg cgcgggaaga
     1861 tgccgccgcc ctcggagatg atgctctcgt cgtagtccgt ccacgaggag cgggggagcc
     1921 tgaagaggcg ctcgcgctcc tcgaagctct tctcgggatc ggggtcggga tcgaggaaga
     1981 tgtgcatgtg gttgaaggcg cccacgagct ttatgtgacg gctcaggagc atgccgttgc
     2041 cgaacacgtc gccggacatg tccccgatgc caacgacggt gaagtcctcg gtctggatgt
     2101 tcttgccgag ttcgcggaag tgccggctca ccgattccca ggcgcccctc gccgttatac
     2161 ccatctcctt gtggtcgtag ccgaccgatc ctcccgaggc gaaggcgtct ccgagccaga
     2221 agccgtactc ggccgagagt tcgttggcta tgtcagagaa cgttgcggtg cctttgtccg
     2281 cggcgacgac gagatatggg tcgtcatcgt cgtagcgtgt aacgtcgggc ggcgggacga
     2341 tgtcatcgcc cgagatattg tccgttatat ctagcatgcc ccggatcagg gtcttgtaac
     2401 aggcgactac ctcctgctgc agagcctcgc gccctccttc tgccggtggg cgtttgacga
     2461 cgaaaccgcc cttcgccccg actgggacga tgacggcgtt cttgaccgtc tgggccttca
     2521 tgaggccgag tatctcggtt ctgaagtcct cgcgcctgtc cgaccagcgg attccgccgc
     2581 gggcgacctc tccgccgcgc aggtgcaccc cctcggtgcg tggggagtag acgaagatct
     2641 cgaagcgggg tagcggtttc ggcatgccgg gaatcttctc tgggtccagc ttgaaagaga
     2701 tgtgtggttt gggatctcca tccggcgtgc tctggtagta gttcgtccgc aacgtcgcca
     2761 gggttatatt gaggaacgag cgcaggatgc ggtcctcatc gaggctcacg accgcctcga
     2821 gggcttcttc gatctcctct tctaaccgct cgctctcggt cctgtcccgg cgcgacgggt
     2881 cgaggcgggc ttcgaagaga tccacgataa gcctggcgat gtgctggttg ttgacgaggg
     2941 cgtcctccat gtaatcctgg gagaacgtcg tgcctgtctg gcgcaggtac ttgcagtagg
     3001 ccctgagcat cgagatctca cgccaggtaa ggcgggcgcg caacacgagg cggttgaagc
     3061 cgtcgttctc tacggtcccg cgccaggcac gggcgaaggc gtcctggaag atctccctga
     3121 cctcacccgt ctgcagctcg tcgtcggtct cgtggacgag gccgaagtca tatatccaga
     3181 ctggaggaga gccctccggc ttgacctcgt gcgggcgttc gtcgaccacc tcgacgccca
     3241 tgtcctccag cagcggcaga atctccgaaa gcgagacctg ctccccgagc ttgaacagct
     3301 tgaaaccaag gaagtcctcg ggctcttcga tgggatggta gaggctcatc tccaggtcat
     3361 cgttggactt cagcctctcc atccgctgga tgtccgagac ggctgtgcgg ggcaggaagc
     3421 ctgcgcggta gccaggggag aaggcgtcgc ggtacttgcg gaacagctct gtgccccgtt
     3481 cctcgccgta ctgttcgatc agggcgtcgt agaggttgtc cgtccaggag cgggtcgtct
     3541 cgacgaggcg gccttcgatc tcttcctcat cgtattccgg tttctccccc ggcttcgtgt
     3601 agattgtgaa gtggacgcgc gcgagcacgg actcagagag tctgacgttg tactctacgt
     3661 ttacgccatc gaaagccttg aggagtatgt cttgcatgcg ttcccggatc aatgtgttgt
     3721 agcggtcgcg gggaacgaag acgaggcagg agaagaagcg cccgtaagtg tcgcgcctca
     3781 cgaagaacct gacgcgctgg cgctcctgca ggtggagtat gcccatggct atctcgaaga
     3841 gctcttcctt ggagacctgg aacagctcgt cgcgcgggta ggtctccagg atctcgatca
     3901 gatccttctc gttgtggctc ccctcgggga agccccccct ctccagaacg taacgcacct
     3961 tgcgcctgac gagggggatg tcgaaggcgc tcgcgctgta ggcggagaag gtataaagcc
     4021 ccaggaaccg ccgctctccc gtgaccgcgc ccgactcgtc gaatttcttg ataccgatgt
     4081 agtccaggta tgaaggcctg tgcaccgtgg cccgcgagtt cgccttcgtc aggttcagca
     4141 gctttggcgt gcgggctagc cgcctcaccc ctggcgggag ctcggcgaaa ctccgggaga
     4201 ccggccccga ttccgtctgc ctcaggatgc cgagccccga cccctgcacg gcgcagaggg
     4261 cttcttcgcc gtcctgcgag atcaggtcgt actcgcggta accgaggaac gtgaagttat
     4321 cgtcgtcgat ccactccagg aacgcgcggg tctcggccag gtcctcaggc tcgaagtcgg
     4381 gcgggctctc ttcga
//
LOCUS       DASTKM010000152        16805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_215216, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000152 DASTKM010000000
VERSION     DASTKM010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16805
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215216"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VFQ10_06335"
     CDS             <1..1160
                     /locus_tag="VFQ10_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HET9964994.1"
                     /translation="VGTVATVQLFTDYLTRLMRYVEFMKRVVPMHDDLFDFFYQALPG
                     YEEVGRRRVQLGCWGSLNDPDACDFRYETMTDWGRKMFVTPGVVVDGKLVTNDLTKIN
                     LGIRILLGSSYYEDWEDQPMFVTHDPVGNPVDRRHPWNQHTIPKPQKRDFDNNYSWVM
                     SPRWFDGTDHLALDTGGGPIARLWATALSGLVDIGYIKATGNSVVINLPRTATKPEAT
                     FEWKIPQWSNTIERDRARVYFIAYAASMAFYFLDRALELLRAGETKVFQEFEVPDEAI
                     GCGFHEAVRGVLSHHMVIRDGKIANYHPYPPTPWNGSPTDSYGTPGPYEDAVQGQPIF
                     EENGPDDFKGIDIMRTVRSFDPCLPCGVHMYSGKGKILEARHSPTLGLGVE"
     gene            1234..2160
                     /locus_tag="VFQ10_06340"
     CDS             1234..2160
                     /locus_tag="VFQ10_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET9964995.1"
                     /translation="MLIDDKGLQERVTRMETLLEEIEVLSDRNARSKAAEVVGVLLDL
                     YGEGLARMMEVVAEGEAREKTFDAFAEDELVSHLLLLHGLHPLDLETRVVRALEEVRP
                     YLQSHGGNVELLGVEEGVARLRMQGSCSGCPSSTVTLKLAIEEAIEKAAPDLEGIEAE
                     GVAEEPKPAPTIIAGPTLRKKEKKRPQEDGASWTVVGGLPQLSSGGMLVKEISDEPVL
                     FLKLGDDYYAYRHLCPGCGELLEEGRLAEAELTCSGCSHRYDVRRAGKCLDEPQLHLE
                     PIPLLVDDVAVEERQGQGWRSIVKIALPSAVG"
     gene            2168..2839
                     /locus_tag="VFQ10_06345"
     CDS             2168..2839
                     /locus_tag="VFQ10_06345"
                     /inference="COORDINATES: protein motif:HMM:NF040271.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HET9964996.1"
                     /translation="MVEQGNRKAFSTFSSLRRFAREESENATKAAEVAQECCDLCGEP
                     LPSEHRHLLEVSTREIMCVCRPCSILFDSEAASEGRYRLVPGRHLFLEDFEMSDVQWE
                     SLMIPVDMAFFFYSTPAERVVAFYPSPMGPTESLLKLSSWEELEKYNPVLEGMERDVE
                     ALLVNRVRGAREHFLVPIDECYSLVGLIRMHWRGLSGGREVWEKIDRFFEELRKRSKT
                     IRRGS"
     gene            2895..3131
                     /locus_tag="VFQ10_06350"
     CDS             2895..3131
                     /locus_tag="VFQ10_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964997.1"
                     /translation="MGDIRVGKPDTTHDAPAHTPGVNQGNEPGGTDADPGLVETGEIG
                     AGRPSMVSTARKSTGINPEKRNPIDPNSPNLPPA"
     gene            3179..3826
                     /locus_tag="VFQ10_06355"
     CDS             3179..3826
                     /locus_tag="VFQ10_06355"
                     /inference="COORDINATES: protein motif:HMM:NF039986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HET9964998.1"
                     /translation="MPDLEFRVEGAEVPEFAAVPSILFKLRIENLDGEPIRSVALNTQ
                     IRIAATRRHYDDAEQERLLELFGEPSRWKDTLRSLLWTHTVLQVPRFSGSTVVDMPVA
                     CTYDLEVVAAKYFYALEHGEVPLEFLFSGTIFYAGEGGKLQTTRISWEKEAGFQFPVR
                     LWKEMMDQYFPNSAWIRLRRDVFDQLYDYKVRKGLPTWEATVEALLRASEQEVER"
     gene            3828..4838
                     /locus_tag="VFQ10_06360"
     CDS             3828..4838
                     /locus_tag="VFQ10_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9964999.1"
                     /translation="MDAVRKIAEAVLYEGYILWPYRRSTTKNQQRWTFGGVYPRAYSE
                     ARGEDDPWLMQTQCLIAGEEEAQVEVRVRFLHVTERKVGIKSGEILELVEELRIGEDL
                     YLPWDEATEWEIAVGRFSLSELVDTPVRVGIDIPGGSSQEAVRSPTGEVEGALVRSWR
                     ALCGTVEVEAEALRDRIFRLTVRITNTTPWEGEDRESTLRQTFCSTHTTLEVSGGEFV
                     SMTDPPEELRGFAEGCENLKTWPVLVGEQGNRTMLLSSPIILSDYPEIAPESPGDMFD
                     GGEIDQLLILNILTLTDEEKAEMRASDPRAREILERCESLPQEDLMNLHGAIREFRVL
                     RD"
     gene            4840..5151
                     /locus_tag="VFQ10_06365"
     CDS             4840..5151
                     /locus_tag="VFQ10_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965000.1"
                     /translation="MDFFREQEERPTLQSVTVDGVELVKGSRVMLWPRAGGDIMDLAL
                     KGKVAFVEAIEQDYEERVHLSVTLEDDPGRDLGDAHILGHRFFFSPDEVVPLDPEAAD
                     E"
     gene            5144..5683
                     /locus_tag="VFQ10_06370"
     CDS             5144..5683
                     /locus_tag="VFQ10_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HET9965001.1"
                     /translation="MSRRILVAGIGNVFLGDDGFGVEVVGRLSERKLPEGLEVVDFGI
                     RGMDLAYALHEDYDLVVFVDATPRDGEPGTVYLIEPEIEEDGAVSLDTHGMDPVRVIR
                     LARALGARPARTVVVGCEPQVIPGGEEYDDMLMELSEPVRGAVEEAAKLVESLVTDDG
                     TDRGPGKSAGSSTESEVKS"
     gene            5875..8208
                     /gene="hypF"
                     /locus_tag="VFQ10_06375"
     CDS             5875..8208
                     /gene="hypF"
                     /locus_tag="VFQ10_06375"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET9965002.1"
                     /translation="MKAPVRERRGVTVRGIVQGVGFRPFVYALARRHGLSGMVRNDAE
                     GVSIEVEGAPEELERFVRVLEEDPPPLATVESVFWQPLATLGDVEFRIEESREGLRRQ
                     ALVSPDVATCDDCLAELLDLTDRRYRYPFTNCTNCGPRFTITRTVPYDRATTTMSRFT
                     MCPDCRREYEDPADRRFHAQPNACPVCGPQVRLLDRFGHELYDGPDDPIARAAQMLRG
                     RAIVAIKGLGGYHLACEPFDEKAVRTLRGRKVRQDKPFALMAGGLAQVREICFVSPDE
                     EALLTSPARPIVLLERRECSGVAEEVAPRQNTLGVMLAYTPLHHLLLRDAGIPLVMTS
                     GNNADEPIAYRDEEALDQLCEIADYFLIHDREIHMRCDDTVVRTAGGRPYPVRRSRGY
                     APAPLRLAADFSRHTLACGGELKNTFCLVRDRHAFMSHHIGDMENYETLRSFREGIEH
                     YCRLFDVSPELVAYDLHPEYLSTKYARELEETGLPALGVQHHHAHVASCLADNERPGD
                     EHVIGVALDGTGYGIDGAIWGGEFFEGSLEKGFTRRAHLEYAPMPGASAAIRQPWRMA
                     LAQLIKLYGEEDTLRLRLAAVGEAGERNVRLISRLVEHKLNTPPTSSAGRLFDAVAAL
                     VGLPGSRHTTYEGQAAIELELIAHAPTNRAYPISLRPEAVAWIVETSEVVAAVVEDLI
                     AGRPIPEISATFHHTMALVVAMVCERIRESEGVSTVALSGGTFQNMLLLTQTTGLLEE
                     KEFVVHRHQRVPTNDGGLALGQAILADTVLREKGLKC"
     gene            8227..8505
                     /locus_tag="VFQ10_06380"
     CDS             8227..8505
                     /locus_tag="VFQ10_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET9965003.1"
                     /translation="MCLGIPGRIVEIIDEDLLLAKAEVSGVRRNINIGLVHNEDEQVE
                     IGDWVLIHVGFAMSRLNETEAQSQLQALEDIGEAYEQELAELRASQIE"
     gene            8508..8774
                     /locus_tag="VFQ10_06385"
     CDS             8508..8774
                     /locus_tag="VFQ10_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET9965004.1"
                     /translation="MEQANPQNSYCTLDHDGCVVCSDTGIPLRVISIEGDDALCEDAV
                     GNRTEIAVELVAPVTTGEVLLTHGGVAIGKVQRADKLPTEGRQP"
     gene            8771..9898
                     /gene="hypD"
                     /locus_tag="VFQ10_06390"
     CDS             8771..9898
                     /gene="hypD"
                     /locus_tag="VFQ10_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HET9965005.1"
                     /translation="MKYVDEFRNAEIARAVAAEIAAITDPARHYKLMEVCGGHTHTIY
                     RHGIKDLLPDQIELVHGPGCPVCVLPMGRVDDGIAVAERPEVIFTCFGDMMRVPGSKG
                     NLLEVKAEGADIRMVYSPLDALRVARENPDREVVFFAIGFETTAPSTALTLQRAKKEG
                     LENFSVFCNHVTIVPPVRALLDSPDLRLDAFIGPGHVSTVIGCRPYEFMPEDYGKPCV
                     VAGFEPLDLLQSILMLMRQLEEGRCEVENQYSRIVPWEGNLAALKSMAEVFELRPYFE
                     WRGMGFIAQSALKLSDAYADFDAELRYSVPGVRVADPKACQCGEVLKGVLKPWECKVF
                     GTACTPEHPLGTLMVSSEGACAAYYNFGRQSFEKVEIGTKQ"
     gene            9895..10971
                     /gene="hypE"
                     /locus_tag="VFQ10_06395"
     CDS             9895..10971
                     /gene="hypE"
                     /locus_tag="VFQ10_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HET9965006.1"
                     /translation="MSEREQRVLDALEQNRRRHPTFTSPRIDMSHGAGGKATHKLIEG
                     LLLPTLENPALVPLADAAMLPFGDASIALTTDSFVVRPLVFPGGSIGELAVNGTVNDL
                     AVSGATPMGLSAALIIEEGLATDVLTHEIEAMARSAEKAGVSIATGDTKVVERGKGDG
                     LYIVTTGVGVADQELKLSSAAVRPGDKILCSGTLGDHGAAILIARGDLELEAEVFSDT
                     RPLTSLAKALMIAAGPDLRFMRDPTRGGVGTVLNELARDSGYGVVLWEEKLPIRDVVH
                     GACEILGIDPLYVANEGKLLAVVAPDQAEAALESLRNTEGGEEAQVIGEVREEPARMV
                     VMHTGFGGTRMIDMLVGDPLPRIC"
     gene            11022..11501
                     /locus_tag="VFQ10_06400"
     CDS             11022..11501
                     /locus_tag="VFQ10_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965007.1"
                     /translation="MNRESPTDLSEISWRHLFTRFPEWQDHAQFVESEASDDLESLYV
                     RVYSPADEKSYLSILARDDCIEISFSDGVPPGGAESQILCEEGSESLCVAAAIEYVQE
                     IIDEKVVIGRERSTRLTEHKTLPPQFIASSEIEAKRRRLVSVRSWKGRYNWEQARPT"
     gene            11620..12801
                     /locus_tag="VFQ10_06405"
     CDS             11620..12801
                     /locus_tag="VFQ10_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9965008.1"
                     /translation="MKQADTDILAIRSREELLRRNQIFESFFDAEAPRLAEACHEMSR
                     RFLAGGRLLAFGNGSAATDAQHVSVEFVHPVIVGKRALPALDLGPDFERRLPVLLRPE
                     DMVMGFAFPEADEPVERAVQAARERGALTFSLTGEAGEYSFSPPDGDPFVVQEVFEVL
                     YHVLWETVHVYFEHREQGHDVGNSSFLYPFLGKGQQPLEEVVRDVQGSMLQKMQEVNT
                     LRAAAAESQANAIAHIAVAVTERLERGGKLITFGNGGSATDANDLVSDCVDPPQGFGT
                     IPAVSLSAEPANITAIANDIGTEAIFARQLIAHAKPEDVAVGISTSGGSANILASLAE
                     ARKRGLLTVGIVGYDGGRIVAERLADYAVVVRSDYIPRIQEVQASIYHVLRGVVEWRM
                     A"
     gene            12830..13021
                     /locus_tag="VFQ10_06410"
     CDS             12830..13021
                     /locus_tag="VFQ10_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965009.1"
                     /translation="MRYALLIAGGLMILMGGIWLLQGIGILPGSFMTGQMFWAVMGAA
                     FLAVGGLVILAGFRLNGRR"
     gene            13132..14457
                     /gene="hemL"
                     /locus_tag="VFQ10_06415"
     CDS             13132..14457
                     /gene="hemL"
                     /locus_tag="VFQ10_06415"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET9965010.1"
                     /translation="MQGSGESVDARTKLAMDRSARLMERAVGLMPGGVNSPVRAFRPV
                     GGDPLFMERGEGSRIFDVDGNSYIDYVMSYGPLVLGHSYPEVIEAIETTARKGTTFGA
                     PTELEVELAELVCGAFPSIEMVRMTNSGTEATMSAIRLARGYTGRERILKFDGNYHGH
                     GDALLVSAGSGVATLGLPDSPGVTSGAARDTAVLPYNDLGAVRDLFAREGEEFAAIIL
                     EPVAGNMGCVPPKEGYLEGLRKITNDYGTLLIFDEVMTGFRLARGGAQERFGVTPDLT
                     CLGKIIGGGLPVGAYGGRREIMDQVAPSGPVYQAGTLSGNPLAMAAGLATLRAAGQPG
                     FYDRLEELGARWREGMSEAASAGSRPCTINQVGSMVSIFFTGGPVTDFRSATASDTGA
                     FKDFFWHMLSRGVYLAPSQYEAGFISTAHSEVDFQRTFEAAREWFDGRD"
     gene            14458..14889
                     /locus_tag="VFQ10_06420"
     CDS             14458..14889
                     /locus_tag="VFQ10_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965011.1"
                     /translation="MEETAGRTPELSLEDTFLKAVAGLPEEDERAGVVAEALGYLQQG
                     FDAHYARGRATDDDVLVGDNAYALAVETIARLDEPRFVAVASRMIRDGAGRIAAGGAV
                     SLHTWTPHLAGLLHIISEEGEQRSEARIREAAEELRGRSSG"
     gene            14870..15613
                     /locus_tag="VFQ10_06425"
     CDS             14870..15613
                     /locus_tag="VFQ10_06425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HET9965012.1"
                     /translation="MGGVVGNGTRNKLPSGAQRALLVREMFDRIAGRYDLMNTLMTAG
                     LNRLWNRKVVRATDLRAGGRALDLACGTGTLTRHLAKKVGPEGYVLGVDFSREMLHAA
                     QSAPNVEYRLGDATDLQDVPSGAFHAATIAYGARNIPDLDALFSEMARTLRPGGVAVC
                     LEIARPTGRVSSAFYGVWFDKIVPMLGGWISGDPSAYAYLPQSVKEFVAPGELADIIG
                     RNGLQNVTWERFAGGIITLHRGIRSNDAG"
     gene            15528..16577
                     /locus_tag="VFQ10_06430"
     CDS             15528..16577
                     /locus_tag="VFQ10_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9965013.1"
                     /translation="MDYKTLHGSASQAASSRSIVGSGQTTQASRLHTALPEGTFETID
                     EVEGLLVGVAGSAPADLVEPALGALTSGGKRLRPLLLVLSARMGEPGQEDLLGAASAI
                     EVLHTATLIHDDIVDRAKSRRGVPTTVAAYGCELAAATGDYLFAEAFSELARIGDPRL
                     IRAFAEASVGLAVGELEQYRANGATVDVEAYLEHIRKKTAGLFKAACVAGGTLGGLSL
                     VQIDALASYGQALGIAFQMSDDIMDLVGKPGLMGKGIGTDLVEGTVTLPVIFALSEGE
                     AGKIRRVLADPSPTPELLEAGIEAVLATDAVARTEEWAKGEIDAALEDLDLLPVSPER
                     EFLESVASEVVGRDA"
     gene            16570..>16805
                     /locus_tag="VFQ10_06435"
     CDS             16570..>16805
                     /locus_tag="VFQ10_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965014.1"
                     /translation="MPESHAGPVIFAAGTLMPISSPPLEDGAVLVIEGRIEAVGAIAE
                     VGRENPDAEVRFFPNYTIIPGAVNAHAHLGFRRE"
BASE COUNT         3358 a         5112 c         5479 g         2856 t
ORIGIN      
        1 gggtcggcac ggtggccacg gtgcagctgt tcaccgacta cctgacccgc ctgatgcgct
       61 acgtggagtt catgaagcgg gtcgtgccca tgcacgacga cctgttcgac ttcttctacc
      121 aggccctgcc cggctacgag gaggtcggcc ggcgccgggt ccagctcggc tgctggggca
      181 gcctgaatga ccccgacgcc tgcgacttcc gctacgagac catgaccgac tggggccgca
      241 agatgttcgt gaccccgggc gtggtcgtcg acggcaagct cgtcaccaac gacctgacca
      301 agatcaacct gggtatccgc atcctgctgg gcagctccta ctacgaggac tgggaagacc
      361 agccgatgtt cgtgacccac gacccggtgg ggaacccggt cgaccgccgc catccctgga
      421 accagcacac catccccaag ccccagaagc gcgacttcga caacaactac tcctgggtga
      481 tgtcgccgcg ctggttcgac ggcaccgacc accttgccct ggacaccggc ggcgggccga
      541 tcgcccggct gtgggcgacc gccctgtccg gcctggtcga catcggctac atcaaggcca
      601 ccggcaacag cgtggtcatc aacctgcccc ggacggccac caagcccgag gccaccttcg
      661 agtggaagat cccccagtgg tccaatacca tcgagcgaga tagggcccga gtctacttca
      721 tagcctatgc ggcgtcgatg gcattctact tcctggatag ggccctggag ctcttgcgcg
      781 cgggcgagac caaggtcttc caggagttcg aggtgccgga cgaggccatc ggctgcggct
      841 tccacgaggc ggttcgcggg gttctctcgc accacatggt gatccgagac ggcaagatcg
      901 ccaactacca cccctatccg ccgacgccgt ggaacgggtc tcctaccgac tcctacggca
      961 cacccgggcc ctacgaggat gcggtgcagg gacagccgat cttcgaggag aacggccccg
     1021 atgacttcaa gggcatcgac atcatgcgca ccgtgcgcag cttcgatccc tgtctgccct
     1081 gcggggtcca tatgtactcg ggcaagggca agatactcga ggcgcggcac tcgccgactc
     1141 tggggttggg cgtagagtag ggcccggcac caggcggatg tgtaaaaggc gggtcccgct
     1201 tgtaaggggc ccgctttcga gaagggtgtt ggggtgctta tagacgacaa gggactacaa
     1261 gagcgcgtca ccaggatgga gacgctgctg gaggagatcg aagtgctctc ggaccggaac
     1321 gcgaggtcca aggccgccga ggtagtaggg gttctcctgg atctctacgg tgagggcctg
     1381 gcgcgtatga tggaggtggt cgccgaaggg gaagcacgtg agaagacctt cgatgccttc
     1441 gccgaagacg aactggtctc gcacctgctg ctcctgcacg ggctgcaccc gttggacctg
     1501 gagacgcggg tggtcagggc cctcgaagag gtgcgtccct acctgcaatc ccacggtggg
     1561 aacgtggagt tgttgggcgt ggaggaaggc gtggcgcgac tgcggatgca ggggagttgc
     1621 agcgggtgcc cttcctccac cgtgacgctc aagctggcga tagaggaagc catcgagaaa
     1681 gccgccccgg atctggaggg gatagaggcc gagggcgtgg ccgaggagcc caagccggcg
     1741 ccgaccatca ttgcgggccc caccctacgc aagaaggaga aaaagcggcc acaagaggac
     1801 ggagcctcct ggacggtcgt cggcgggctt ccccaactct cgagtggcgg gatgttggtg
     1861 aaggagatct ccgacgaacc tgtactcttc ttgaagctgg gggatgacta ttacgcctac
     1921 cgccacctct gccccggctg cggagaattg ttggaggagg gtagattagc cgaggcggaa
     1981 ctcacttgct ccgggtgctc tcaccgctac gacgtgcgcc gggccgggaa atgcctggac
     2041 gagccgcagc tacatctgga gcctatcccg ctgttggtgg acgacgtagc cgtcgaggag
     2101 cgtcagggcc aggggtggcg aagcatagta aagatcgccc tcccgagcgc ggtgggctga
     2161 ggggtatatg gtcgagcagg gcaacaggaa ggccttctcc actttctcct cgctacgccg
     2221 gttcgccaga gaagagtccg agaacgcgac aaaggcggcg gaggttgcgc aggagtgctg
     2281 cgacctgtgc ggcgagccat taccgtcgga gcatcggcac ctgttggaag tctccacgcg
     2341 ggagatcatg tgcgtctgcc ggccgtgttc gatcctcttc gacagcgaag cggcgagcga
     2401 gggcagatac cgcctcgtac ccggccgcca cctcttcctc gaagacttcg agatgagcga
     2461 cgtacagtgg gagagcctca tgataccggt ggatatggcg ttcttcttct acagcacgcc
     2521 cgccgagagg gtcgtggcgt tctatccaag cccgatgggt ccgacggaat cgctcctgaa
     2581 gttgagctcc tgggaggagc tggagaagta caatcctgtg ctggagggca tggagcggga
     2641 tgtagaggcg ctcctcgtaa accgggtaag gggagcgcgg gagcacttcc tggtcccgat
     2701 cgacgagtgc tacagccttg tgggactcat ccgaatgcac tggaggggcc tgagcggtgg
     2761 cagggaagtc tgggagaaga tagacaggtt cttcgaggag ctaaggaagc gttccaaaac
     2821 cattcggagg ggaagttgaa ggctcgcccg ccgattgagg gcaagacaaa gactaccagg
     2881 aaaggagacg gtagatgggg gatataaggg taggtaaacc ggacacgacg catgatgcgc
     2941 ccgcccatac gccgggggta aaccagggca acgagcctgg tggtaccgat gcggatcccg
     3001 gcctcgtaga gaccggcgag atcggagcgg ggcgtccaag catggtgtct acggcccgga
     3061 agtctacggg catcaacccc gagaaaagga accccataga cccgaatagc ccgaacctgc
     3121 cgccggcttg atgaggcgtc gatacgacga cagagccagc ttctgatggt agtcggctgt
     3181 gcctgacctg gagttcaggg ttgagggcgc tgaagtcccc gaattcgccg ccgtgccgag
     3241 catactcttc aagctgcgta tcgagaacct ggatggtgag cctatccgct ccgtcgctct
     3301 gaacacgcag attcgtatcg cggcgaccag gcggcactac gatgatgcgg agcaagagag
     3361 actcctggag ctgttcggcg agccgagcag atggaaggat acattgcgga gcttgctctg
     3421 gacccacacc gtcctgcagg ttccccggtt ctcggggagc acggtcgttg acatgcccgt
     3481 tgcgtgtacc tatgacctcg aggtcgtcgc ggccaagtac ttctatgccc tcgaacacgg
     3541 tgaggtaccg ctcgagttcc tgttcagcgg cacgatcttc tacgcgggag aaggggggaa
     3601 gctgcagacc acaaggatct cctgggagaa agaggcgggg ttccagtttc ccgtaaggct
     3661 gtggaaagag atgatggatc agtatttccc gaacagcgcc tggatcaggc tccgcaggga
     3721 cgtctttgac cagctctacg actacaaggt acgcaagggc ctgccaacct gggaagccac
     3781 cgtagaggcg ctgctgcggg cgagcgagca ggaggtggag cgctagagtg gacgcggtga
     3841 gaaagattgc cgaggccgtg ctctacgaag gctacattct ctggccctac cgccgatcca
     3901 cgacgaagaa ccagcagcgc tggacgttcg gaggggtgta cccccgtgca tacagcgagg
     3961 ccaggggaga agacgacccc tggctgatgc agactcagtg cctgatagcg ggagaggaag
     4021 aggcgcaagt cgaggtaagg gtgcgcttct tgcacgtaac cgaacgcaag gtcggcataa
     4081 agagcggcga gatcctcgag cttgtcgaag aattacggat cggggaagat ctctatctgc
     4141 cgtgggacga ggcgaccgag tgggagatag cggtcggacg gttcagcctc tctgaactag
     4201 tcgacacacc cgtacgggta ggtatagaca ttcccggagg gagcagtcag gaagcggtca
     4261 ggagtccaac gggcgaggtc gagggagctc tcgtgcgtag ctggcgcgcg ttgtgcggaa
     4321 cggtcgaggt agaggccgag gctttgcggg acaggatctt cagactgacc gtcaggatca
     4381 ccaacactac cccctgggaa ggggaggata gggagagcac gctgcgtcag accttctgct
     4441 cgacccacac gacgctcgaa gtctcagggg gagaattcgt ctccatgact gaccctcctg
     4501 aggagttgcg ggggttcgcg gaagggtgtg agaacctcaa gacgtggccc gtgctcgtcg
     4561 gggagcaggg taacagaacc atgctgctct cctccccgat catactctct gactatcccg
     4621 agatcgcccc agaaagcccg ggcgacatgt tcgacggcgg ggagatagat cagctcctga
     4681 tcctgaacat tctcaccctc actgatgagg agaaggcgga gatgcgtgcc tcggacccgc
     4741 gggcgcggga gatcctggag cggtgcgagt ccctcccgca ggaggatctg atgaacctgc
     4801 acggtgccat ccgcgagttc agggtgttga gggactagga tggatttctt cagggaacag
     4861 gaggaaagac ccacgctcca gagtgtgacc gtggacggcg tcgagctcgt caagggtagc
     4921 cgtgtcatgc tctggccccg cgctggaggg gacatcatgg atttggctct caagggaaag
     4981 gtcgcgttcg tcgaggctat cgagcaagac tacgaagaga gggtccacct gtccgtcacg
     5041 ctggaagacg atcctggcag ggacctggga gacgcccata tcctggggca caggtttttc
     5101 ttctcgcccg atgaggtcgt gccgctcgac cctgaggccg ccgatgagta ggcgcatcct
     5161 cgtcgccggc atcggcaacg tcttcctcgg cgacgacggc ttcggggtcg aggtggtagg
     5221 gcgactctcc gaacgcaagc tgccggaggg cctcgaggtc gtggacttcg gcatccgtgg
     5281 aatggatctc gcgtacgccc tgcacgaaga ttacgacctg gtcgtcttcg tcgacgccac
     5341 cccgcgtgac ggggagcctg gcaccgtcta cctcatcgag ccggagatcg aggaagatgg
     5401 agcggtctcg ctcgacaccc acgggatgga ccctgtcagg gtcatcaggc tggctcgcgc
     5461 cctgggcgcc cgaccggccc gaaccgtcgt ggtcggctgc gagccgcagg tgatcccagg
     5521 cggagaagaa tacgatgaca tgctgatgga actgagcgaa ccggtacgcg gggcggtgga
     5581 ggaagccgcg aagctggtgg agtcactcgt tacggacgat ggcacggaca gaggacccgg
     5641 aaaatcggcc gggtcctcca cagaaagtga ggtgaagtcg tgaagaagct gttgttcctc
     5701 ggagtattac tgggggctgg agccgccgta gccgcgcagt ggccggagat ccaacgttac
     5761 ataaagatgc gcaacatgta gagcgctggg ggtacgaact gcgaacgcac gctactagac
     5821 cgttgaacag agacgctaaa tgaataccag cgcctggcca cgatgcctta caccatgaaa
     5881 gcccctgtcc gcgagcgccg cggggtcacc gtccgtggca tcgtgcaggg ggttggcttt
     5941 aggcctttcg tctacgcact ggcgcgcagg cacgggctct cggggatggt ccgcaacgac
     6001 gctgagggcg tcagtatcga ggtcgagggc gcgccggagg agctcgaacg ctttgtgcgc
     6061 gtgctcgagg aggaccctcc tcccctggcc acggtcgagt ccgtcttctg gcaaccgctc
     6121 gcgaccctcg gggacgtaga gttccgcatc gaggagagca gggagggact ccgccgtcag
     6181 gccctcgtct cgccggacgt cgcaacctgc gacgattgcc tggccgagct tctggacctc
     6241 acggatcggc gttaccgtta ccctttcacc aattgcacga actgcggccc gcgcttcacc
     6301 atcacccgca cagttcctta cgacagggcc acgactacga tgtctcgctt tactatgtgc
     6361 cccgactgcc gccgcgagta cgaggatccc gccgaccgcc gcttccacgc ccaacccaac
     6421 gcctgtcccg tctgcggacc gcaggtaagg ctgctcgaca ggttcgggca cgagctttac
     6481 gacggcccgg atgaccccat tgcacgcgcc gcgcagatgc tgcgtggcag ggctatcgtc
     6541 gccataaagg gtctcggcgg ttaccatctg gcctgcgagc ctttcgacga gaaggctgta
     6601 aggaccctgc ggggccgcaa ggtgcgccag gacaagccct tcgcgctcat ggcaggcggc
     6661 ctcgctcagg tacgtgagat ctgcttcgtc tcccctgacg aagaggcgct gctcacctcg
     6721 cctgcacgcc ccatagtcct cctggaacgt agggagtgtt ccggtgtcgc cgaagaggta
     6781 gcccctcgcc agaataccct gggcgtgatg ctcgcttaca cgccgctgca ccacctgctt
     6841 ttgcgcgacg cgggcatccc gctcgtcatg accagcggca acaacgcgga tgagcccatc
     6901 gcctatcgcg acgaggaagc gttggatcag ctgtgcgaaa tcgccgacta cttcctcatc
     6961 cacgaccgtg agatccacat gcgctgcgac gacaccgtgg tccgtacagc gggaggaagg
     7021 ccgtacccgg tccgtcgctc ccgtggctac gcccctgcac cgctccggct ggccgcggac
     7081 ttcagcaggc acaccctggc ctgcggtgga gagctcaaga acacgttctg cctggtcaga
     7141 gaccgccacg ccttcatgag ccaccacatc ggggacatgg agaactacga gacgctgcgc
     7201 tcgttcaggg aaggaatcga gcattattgc aggctcttcg acgtgagccc cgagctggta
     7261 gcttacgacc tccatcccga gtacctatcc accaaatacg cccgcgagct cgaggaaacc
     7321 gggctccccg ctctcggggt acagcaccac cacgcgcacg tcgcgagctg cctcgctgac
     7381 aacgagcggc cgggcgacga gcacgtaatc ggggtcgccc tcgacgggac cggctatggt
     7441 atagacgggg ccatctgggg cggagagttt ttcgagggaa gtctggaaaa ggggttcacg
     7501 cggcgggcgc acctggagta tgcgccgatg ccgggggcat cggcggccat ccgccagccc
     7561 tggcgaatgg cgctcgcgca actcatcaag ctctatggag aagaggatac cctacggctt
     7621 cggctcgccg ccgtagggga agcaggtgaa cggaacgtaa ggctcatctc ccgtctagtg
     7681 gagcacaaac tcaacacgcc gcccacctcg agcgcaggcc ggctcttcga cgccgtagcc
     7741 gcactcgttg gcctacccgg ctccaggcac acgacctatg aaggacaggc agccatagag
     7801 ctcgagctga tcgcccacgc ccccacgaac agggcctacc ccatcagcct gcgccccgag
     7861 gccgtcgcct ggatagtcga gaccagcgaa gtcgtggccg ccgtagtcga agacctgatc
     7921 gctgggcgcc ctatccccga aatctcagcc acgttccacc ataccatggc ccttgtagtc
     7981 gccatggtct gcgaacgaat cagagagtca gaaggagtaa gcacagtcgc cctctcaggc
     8041 ggcaccttcc agaacatgct cctactaacc cagacaacag gcctactgga ggagaaggaa
     8101 ttcgtggtcc acagacacca acgcgtcccc actaacgacg gcggcctggc actaggacaa
     8161 gcaatactgg cggatacagt attgcgggag aaagggctga aatgctaaac acgaagggag
     8221 tcgatcatgt gccttggaat tcccggccgg atagtcgaga taattgacga ggaccttctg
     8281 ctcgcaaaag ccgaagtgag cggggtcagg cgcaacatca acataggcct cgtccacaac
     8341 gaggacgagc aggtcgagat aggggactgg gtcttgatcc acgtcggctt cgcgatgtcc
     8401 aggctgaacg agactgaggc gcagtcccag ctacaggcgc tcgaagatat cggcgaggcc
     8461 tacgagcagg agctggctga gctacgcgcc tcccagatag agtagccatg gaacaggcga
     8521 acccacagaa ttcctactgc acactagacc acgacggctg cgtcgtctgc tcagacaccg
     8581 gcatcccgct tcgagtgatc tccatcgagg gcgacgacgc cctctgcgaa gacgcagtcg
     8641 ggaatcggac cgagatcgca gtggagctag tcgccccagt aacaacagga gaagtcctgc
     8701 tgactcacgg cggcgtcgcc ataggaaagg tccaaagggc tgacaagctg ccgaccgaag
     8761 ggaggcaacc atgaaatacg tggatgagtt caggaacgcc gagatcgccc gcgccgtagc
     8821 agctgagata gcagcgatca cagaccctgc aagacactac aagctgatgg aggtctgcgg
     8881 cggccacacc cacaccatct accgccacgg gataaaggat ctcttaccgg atcagataga
     8941 gctggtacac gggcccggct gcccggtctg cgtcctcccg atgggacgcg tagacgacgg
     9001 gatagccgtc gccgagcgcc ccgaagtcat cttcacctgc tttggggaca tgatgcgcgt
     9061 ccctggctcg aaaggaaacc tgctcgaggt aaaggccgaa ggcgctgaca tccgcatggt
     9121 ctactcaccg ctcgacgccc tgcgcgttgc gcgcgagaac cccgaccgcg aggtggtctt
     9181 cttcgccatc ggcttcgaga ccacggcccc atcgacggcg ctgaccctgc agagggcgaa
     9241 gaaggagggt ctcgagaact tctccgtgtt ctgcaaccac gtaacgatcg tcccgcctgt
     9301 gcgggcgctg ctcgactcgc cggatctgcg cctggacgcg tttatcggac ccgggcacgt
     9361 ctcgaccgtc atcggctgca ggccatacga gttcatgccc gaggactacg gcaagccttg
     9421 cgtggtcgcc ggcttcgagc cactcgacct cttgcagtcc atcctcatgc tcatgcgcca
     9481 gctcgaggag ggacgctgcg aggtcgaaaa ccagtacagc aggatcgtcc cctgggaagg
     9541 caaccttgcg gccctcaaga gcatggccga ggtcttcgag cttcgtccgt acttcgagtg
     9601 gcgggggatg ggctttatag cccagtcggc cctcaagctc tcggacgcct acgcagactt
     9661 cgacgccgaa ctacgctact ccgtaccagg tgtgcgtgtc gccgacccga aggcctgtca
     9721 atgtggcgag gtactcaagg gcgtactcaa accctgggaa tgcaaggtct tcggcaccgc
     9781 ctgcacgccc gagcatccgc taggcacgct catggtctca tccgagggag cctgcgccgc
     9841 ctactacaac ttcgggcgcc agtcgttcga gaaggtcgag atcgggacca aacaatgagc
     9901 gagagagagc agcgcgtcct cgacgcgctg gaacagaacc gccgacgcca cccgaccttc
     9961 acatccccgc gtatagacat gagccatggc gccgggggca aggcgaccca caaactgatc
    10021 gaaggcctgc tccttcctac cctcgaaaac ccggccctcg tcccgctagc cgatgctgca
    10081 atgctcccct tcggcgacgc cagcatcgcc ctgaccaccg actccttcgt tgtgcgcccc
    10141 ctcgtcttcc ccggcggctc tataggggag ctggcggtaa acggcaccgt caacgacctc
    10201 gccgtctcgg gcgccacccc tatgggcctc tccgccgccc tcatcatcga ggagggactc
    10261 gcaacagacg tacttaccca tgagatcgaa gctatggcga gatccgccga gaaggcaggg
    10321 gtctccatag caacgggcga caccaaagtg gtggagcgcg gcaagggcga cgggctctac
    10381 atcgtcacta ccggagtagg cgtcgcggat caggagttga aactctcgtc agccgccgtc
    10441 aggcccggag acaagatcct gtgctcaggc acattgggag atcacggtgc tgcgatcctg
    10501 atcgcgcgcg gcgacctgga gctggaagcc gaagtcttct ccgacaccag gcccctcaca
    10561 tcgcttgcca aagccctcat gatcgccgca ggccctgacc tgcgtttcat gcgtgacccg
    10621 acccgaggcg gcgtcgggac cgtgctgaac gagctcgccc gcgactcagg ttatggcgtc
    10681 gtgctctggg aggagaagct cccgatccgg gacgtcgtgc acggggcctg tgagatcctt
    10741 ggcatagacc cactctatgt cgcgaacgaa ggcaaactcc tcgctgtggt agctccagat
    10801 caagcggaag ccgccctcga atccctgcgc aataccgaag gcggcgagga agcccaggtc
    10861 atcggcgaag tgcgcgagga accggcgcgc atggtcgtca tgcacaccgg cttcggcggt
    10921 acccgcatga tagacatgct cgtcggcgac ccgttgccga ggatatgttg aggagattgt
    10981 ggccctgatc tcgcgggcgg gatgagtaga gatcatgaac catgaataga gaatctccaa
    11041 ccgacctgtc tgagatctcg tggcggcacc tcttcacccg attcccagaa tggcaagatc
    11101 atgcccagtt cgtagagtcc gaagcgagtg atgacctcga gagcctttat gtgcgagtct
    11161 actcaccggc ggacgaaaag agctatctat cgattttggc aagggacgat tgcatcgaga
    11221 taagtttcag cgatggagtg ccgcctggcg gcgccgaaag ccagatactt tgcgaggaag
    11281 gatcggagag tctatgcgtt gcggcggcca ttgaatatgt ccaagagatc atagacgaga
    11341 aggtagtcat tgggcgagag cgttctaccc ggctcaccga gcataagacc ttgcctccac
    11401 aattcattgc ctcgagcgaa atagaagcaa agcgaagaag gcttgtcagc gttcgttcct
    11461 ggaaaggaag atacaattgg gagcaggcaa ggccaacatg atgacacaag ccaacgccca
    11521 agaacgttga gtgctcaacg agccttctag gtcgagacgg ccacacgtct ggcgcggcag
    11581 gcactgtgag tggagcttga cgggctggag gtgtaaacgg tgaagcaggc agataccgac
    11641 atcctcgcta tccgcagcag agaagaactg ctccggcgca accagatctt cgagtctttc
    11701 ttcgacgccg aggccccgag acttgccgaa gcctgccacg agatgtcccg gcgtttcctg
    11761 gccggtggga ggctcctggc attcggaaac ggctctgcgg ctaccgacgc gcagcacgtc
    11821 tcggtcgagt tcgtgcaccc ggtgatcgtg ggcaagaggg cactgcccgc gctcgacctc
    11881 ggtcccgatt tcgagaggcg cctgcccgtc ctgctgcggc ccgaagacat ggtaatgggc
    11941 ttcgcgttcc ccgaggcgga cgagccggtc gagcgggcgg tccaggcggc gcgagagcgg
    12001 ggagcgctga cgttcagcct gacgggcgag gcgggtgagt attcgttctc gccgcctgat
    12061 ggggacccgt ttgtcgttca ggaagtcttc gaggtcctct accacgtgct ctgggagacg
    12121 gtccacgtgt acttcgaaca tcgcgaacag ggccatgacg tgggcaattc ctccttcctc
    12181 taccccttcc tcggcaaagg gcagcagccg ctcgaagagg tcgtgcgcga tgtacagggc
    12241 tctatgctcc agaagatgca ggaggtgaac accctgcggg ccgcggcagc cgagtcacag
    12301 gccaacgcca tcgcccacat agccgtggcc gtcaccgaac gactggagcg tggaggcaag
    12361 ctcatcacct tcggtaacgg cggctcggct acggacgcga acgatcttgt ctcggactgc
    12421 gttgacccac cccagggttt cggcacgatc ccggcggtct ccctgtccgc tgagccggcc
    12481 aacataacgg ccatagccaa cgacatcggc acagaggcga tcttcgcccg ccagctcatc
    12541 gcccacgcga agccagagga cgtagcggtc ggtatctcga cgagcggggg ctccgctaac
    12601 atactcgcct cgctcgccga agcccgaaag cgaggattac tgacggtcgg catcgtgggc
    12661 tacgatggag gtaggatcgt cgctgagcgg ttggcggatt acgcggtcgt cgtccgctcc
    12721 gactacatcc cgcgcatcca ggaggtccag gcttccatct accacgtact tcgcggggtc
    12781 gttgaatggc gcatggcctg agccctggat ctggacctgg aggtatcgta tgcgctatgc
    12841 gctgctgatc gcgggagggt tgatgatcct catgggcggc atctggttgt tgcagggcat
    12901 cggtatattg cccggcagct tcatgaccgg acagatgttc tgggccgtaa tgggcgcagc
    12961 ctttctggcc gttggaggat tggtcatcct cgctggattt cgcctgaacg gacgacgctg
    13021 agagtggtga gaacagcccg ccggttcgcc atgtggggta agctctgtca cggtcggtac
    13081 cggcccaggg taatagactt caaacagacg cagcgaaggg ggttgacgcg tttgcaaggt
    13141 agcggagaga gcgtagacgc cagaacgaag ctcgccatgg accgctcggc caggctgatg
    13201 gaacgggcag tcgggctaat gccgggtggc gtcaacagcc ccgtgcgcgc tttccgcccg
    13261 gtcggcggag acccactgtt catggagcgc ggcgaaggtt cgcgcatctt cgacgtggat
    13321 ggcaactcgt atatcgacta cgtgatgagc tacggtccgc tcgtactcgg gcactcgtat
    13381 ccagaggtca tcgaggccat agagaccacg gccaggaagg gaacgacctt cggcgccccg
    13441 acggaactcg aggtcgaact cgccgagttg gtttgtggag ccttcccttc catcgagatg
    13501 gtcaggatga ccaactcggg gacggaggcg acgatgagcg ccatcaggct cgcccgcggc
    13561 tacacgggcc gggagaggat cctgaagttc gacggcaact accacggcca cggcgacgct
    13621 ctcctcgtct cggccggctc cggtgtggcc accctcggcc tccctgacag cccgggcgtt
    13681 acaagtggtg cagccagaga tactgcagtc ctgccttaca acgacctcgg ggcggtgcgt
    13741 gatctcttcg cgcgcgaggg agaagaattc gccgccatta tcctggagcc cgtcgctgga
    13801 aacatgggct gtgttccccc caaagagggt tatctcgaag gattacgcaa gatcacaaac
    13861 gactatggca cgctcctcat cttcgatgag gtcatgactg gtttcaggct cgcccgtggg
    13921 ggggcgcagg agcgtttcgg cgttacgccc gacctgacat gcctcggtaa gataatcggc
    13981 ggcggactcc ctgtcggtgc atacggtggg agacgggaga tcatggatca ggtcgcaccc
    14041 tcgggccccg tctaccaggc aggcacgctg tcgggcaacc cgctcgcgat ggccgcaggc
    14101 ctggcgaccc tgcgcgccgc agggcagccg ggcttttacg accggctgga agaactcggg
    14161 gcgcgctggc gggaagggat gagcgaagcc gcctctgccg gaagcagacc ctgcaccata
    14221 aaccaggtcg gctcgatggt gagcatcttc ttcaccggcg gtcccgtaac ggacttccgc
    14281 tcagcaaccg cctcggacac cggggcgttc aaggacttct tctggcacat gctctcccgc
    14341 ggggtctacc tggcccccag ccagtatgag gccggcttta tctctacagc ccactctgag
    14401 gtggactttc agaggacctt cgaggcggca cgcgagtggt tcgatgggag ggactaggtg
    14461 gaagagacgg ccggacgcac gccggagctc tcgctcgagg acacgtttct caaggccgtc
    14521 gcaggccttc ccgaggagga cgagcgcgcc ggggtcgtgg ccgaggcact cgggtacctg
    14581 cagcaaggct tcgacgccca ctacgcaaga ggtagggcga ccgatgacga cgtcctggtc
    14641 ggggacaacg cgtacgcgct ggcggtcgag acgatagccc gtctggacga accgcggttc
    14701 gtggctgtgg cgagccgtat gatccgcgac ggcgcaggac gcatcgccgc tggcggagca
    14761 gtctctctac atacgtggac tccccatctc gcaggcctcc tccacatcat ctcggaggag
    14821 ggagaacaga ggtccgaggc gaggatcagg gaggccgctg aggagctgcg tgggcggagt
    14881 agtgggtaac ggaacccgaa acaaattgcc ctcgggggca cagcgggcgc tgctggtgcg
    14941 cgagatgttc gaccggatcg caggccgcta cgatctcatg aacaccctaa tgaccgcggg
    15001 actcaaccga ctctggaaca ggaaggtggt cagggcaacg gacctgaggg caggcggcag
    15061 ggcgctcgac ctcgcctgcg gcacggggac cctcacgcgc cacctcgcga agaaggtggg
    15121 gcctgagggc tacgtgttgg gcgtcgactt ctccagggag atgctgcacg ccgcacaatc
    15181 agcgcccaac gtcgaatacc ggctcgggga tgcgaccgac ctccaggatg taccttccgg
    15241 cgccttccac gcggccacca tagcttacgg tgcgaggaac atccccgacc tcgacgcgct
    15301 cttttccgag atggcgcgta cgctcaggcc gggcggggtt gcggtgtgcc tagagatagc
    15361 gcggccaacg ggcagggtct cctccgcgtt ctacggggtg tggttcgata agatcgttcc
    15421 aatgctcgga ggttggatct ccggtgaccc atcagcttac gcttatcttc cacaatccgt
    15481 gaaagagttc gtagcgcccg gcgaattagc tgacataata ggacgtaatg gattacaaaa
    15541 cgttacatgg gagcgcttcg caggcggcat catcacgctc catcgtggga tcaggtcaaa
    15601 cgacgcaggc tagcaggctc cataccgcgc tacccgaggg caccttcgag accatcgacg
    15661 aggtcgaggg ccttctcgtc ggggtggccg ggagtgcccc tgccgatctg gtcgagccgg
    15721 cgctcggggc ccttacgtca ggggggaaac gtctcaggcc gctgctcctg gtgttgagcg
    15781 cacgcatggg ggagcccggg caggaggacc tgctcggggc ggcgagcgcc atagaggtac
    15841 tccacacggc caccttgatc cacgatgata tcgtggaccg ggccaagagc aggcgcggcg
    15901 tgcctaccac cgtcgccgcg tacgggtgtg agctcgcggc ggccacagga gactacctct
    15961 tcgccgaagc tttctcggag cttgcgagga tcggggaccc taggctcatc cgcgccttcg
    16021 ccgaggcctc ggtggggctc gcagtggggg aactggagca gtaccgcgcg aacggggcga
    16081 cggtcgacgt cgaggcgtac ctggagcaca taaggaagaa gaccgcaggc ctgtttaagg
    16141 ctgcgtgcgt ggccggcgga acgcttggcg ggctctcgct cgtgcagata gacgcgctcg
    16201 ccagttacgg acaggccctt ggcatagcct ttcagatgtc agacgacata atggacctcg
    16261 tcggcaagcc aggccttatg ggcaagggca taggcacgga cctcgtcgag ggcaccgtga
    16321 cgctgccggt catcttcgcc ttgagcgaag gcgaggcggg caagatccgg cgggtcctcg
    16381 cagacccgag cccgactcct gagctgctcg aggcaggcat agaagctgtg ctcgccacgg
    16441 acgccgtggc caggaccgag gaatgggcca agggcgagat cgacgcggcc ctcgaagacc
    16501 tcgacctcct gcccgttagc cccgagcgcg agttcctgga gtctgtggcc tcggaggtgg
    16561 tcgggagaga tgcctgagag ccatgccgga cctgtgatct tcgcggccgg aaccctgatg
    16621 cccatctcgt caccccctct ggaggacggt gcagtgctcg taatagaggg gcgcatagag
    16681 gccgtgggag ccatcgcaga ggtcggacgc gagaaccctg acgccgaggt caggttcttc
    16741 cccaactaca cgatcatccc tggcgcggtc aacgcccacg cccacctcgg cttcaggcgg
    16801 gagga
//
LOCUS       DASTKM010000153         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_744027, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000153 DASTKM010000000
VERSION     DASTKM010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744027"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFQ10_06440"
     CDS             <1..381
                     /locus_tag="VFQ10_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9965015.1"
                     /translation="RLVMDKPQVGLKVMSVLSERLSLAEDLMEDIALKEVPARLASFI
                     LRLVDSEGVVTREGYKVPTRYTHRQLATMIGSKRETVTKAFTLLQQAGIIELRRRRIH
                     IKDIEALKRVADPGLHQTSSRRLL"
     gene            complement(547..1308)
                     /locus_tag="VFQ10_06445"
     CDS             complement(547..1308)
                     /locus_tag="VFQ10_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET9965016.1"
                     /translation="MSALQELVTEWLTSISALLPVGFAFGAGMVAAVNPCGFAMLPAY
                     LSLYLGAREEEFAKRPAGTRALRALLVGFAVSSGFVLLFAAAGVVVAAGGHALLGVMP
                     WVGLSIGVVLSVLGLWMLAGRSLYVGFFQRIAGRLGSPNLASLKGFFLFGLGYGAASL
                     SCTLPVFLLVVGNGIAAGGFLPAMARFMGYALGMASVIVTLTLALAFFKVGLVARFGR
                     AIPYVRLTSAILLLGAGVYLVLYWLTRLTQAAPLG"
     gene            complement(1305..1604)
                     /locus_tag="VFQ10_06450"
     CDS             complement(1305..1604)
                     /locus_tag="VFQ10_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965017.1"
                     /translation="MEEARTWSEILPAYEDRGLNMLMISIDPYETEQSIEAFNAQAGV
                     EDPLPTVIDGGDIARRFGANALETTIILDQNGEEVYRDVTISDAKTLRSELEGVL"
     gene            complement(1614..1898)
                     /locus_tag="VFQ10_06455"
     CDS             complement(1614..1898)
                     /locus_tag="VFQ10_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965018.1"
                     /translation="MARFLTLTALLAAMFVIAACGGGGPQGSERDAPTEPQQSQAAPA
                     PAEDEAAESAEENARRSEESSGQSVTTIEGEEVKLGAQGDVTALFFMAGW"
     gene            complement(1901..>2190)
                     /locus_tag="VFQ10_06460"
     CDS             complement(1901..>2190)
                     /locus_tag="VFQ10_06460"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HET9965019.1"
                     /translation="GTASALDDDAVAAGRDVGSAAVFSRTLDGATLTFRSEGGRIVDE
                     QTGSEWNALGRALEGELTGEQLERVVSIDHFWFSWAAFRPDTRVYKLQGAR"
BASE COUNT          443 a          729 c          655 g          363 t
ORIGIN      
        1 cggctcgtaa tggacaagcc ccaggtcggc ctgaaagtga tgagcgtcct gagcgaacgg
       61 ctctccctcg cggaggacct gatggaggat atagccctca aggaggttcc tgctcgcctt
      121 gcaagcttta tcctcaggct cgtagatagc gaaggggtgg tgacgagaga aggctacaag
      181 gtacccaccc gctacaccca ccggcagcta gcgaccatga tcggctccaa gcgcgagacc
      241 gtgaccaagg ccttcaccct tctgcagcag gctgggatca tcgagttgag gcgccgcagg
      301 atacacatca aggacattga ggctctcaag agggttgcgg acccagggct gcaccaaaca
      361 tcgtctcgta ggctcctctg aactggctgt gtcttgctta cccgaccagg tcgtgaacgc
      421 cacgtcaggc atcggtatgc cccatcctga gatgacgtgg gggctgccga ccaagccctc
      481 acataggcgc ctcttggaga agagcactcg gaaaccgagt gtcagtcaac ctctcaaatg
      541 atcttcctat cctaatggag cagcctgggt aaggcgcgtc aaccagtaga ggaccaggta
      601 cacccccgca cccaacagga ggatcgccga ggtcagtcgc acgtacggta ttgccctgcc
      661 gaaccgggcg acgagtccta ctttgaagaa tgcgagggcc agcgtgagcg tcacgataac
      721 cgaggccatc cccaaggcgt agcccatgaa gcgggccatg gccggcagga agccgccggc
      781 ggcaatcccg ttgcccacaa cgagcaggaa caccggcagg gtgcagctga gcgaggcagc
      841 cccgtatccg aggccgaaga ggaaaaagcc cttgaggctc gccaggttag gggaaccgag
      901 ccgaccagcg atacgctgga agaagccaac gtacaaactc cgccccgcca gcatccacag
      961 gcccagcacg ctcagcacta cgccgataga aagccctacc cagggcatca ccccgaggag
     1021 ggcatgtcct ccggccgcca ccacgacgcc ggccgccgcg aagagcaaca cgaaccccga
     1081 agagacggcg aagcccacca gtagcgcccg caacgccctg gtacccgccg gacgcttggc
     1141 gaactcttcc tcccgagcgc cgaggtagag agagaggtag gcgggcagca tcgcgaaccc
     1201 gcaggggttg actgccgcca ccatccctgc gccgaaagcg aagcccaccg gaagcagtgc
     1261 gctgatgctc gtgagccact cggtgacaag ctcttgaagc gcgctcacag cacgccctcg
     1321 agctccgacc tcaaggtctt ggcgtctgag atggtgacgt cccggtagac ctcttcgccg
     1381 ttctggtcga ggattatcgt ggtctccaac gcgttcgcgc cgaaccgccg ggcgatatcc
     1441 ccgccgtcga tgacggtggg cagggggtct tccactccgg cctgggcgtt gaacgcttcg
     1501 atggactgct ctgtctcata agggtctatg gatatcatca gcatgtttag cccacgatcc
     1561 tcgtaggccg ggaggatctc ggaccacgtc cgagcttcct ccatgcacct agatcaccac
     1621 cctgccatga aaaacagcgc cgtgacgtcg ccctgtgccc ccagcttgac ctcctcgcct
     1681 tcaatggtcg tgacgctctg gccggatgac tcttcgcttc gccgcgcgtt ttcctctgcg
     1741 ctctctgcgg cctcgtcttc cgccggagcg ggcgccgcct gcgactgctg gggctccgtc
     1801 ggggcgtccc gttcggatcc ctgagggccg cctccgccgc atgcggcgat gacgaacata
     1861 gctgccagaa gcgccgtcaa cgtcaagaat cttgccattc tcacctcgca ccttggagtt
     1921 tgtagactcg cgtatcgggg cggaaggctg cccaggagaa ccagaagtgg tcgatgctga
     1981 caacccgctc cagctgctct cctgtgagct cgccctcaag ggcgcgcccc agagcgttcc
     2041 attcgctgcc ggtctgctcg tcgactatcc taccgccttc ggaacggaag gtgagggttg
     2101 ccccatcaag ggtgcgggag aagactgcag ccgaaccgac gtcgcgaccc gccgctaccg
     2161 cgtcgtcgtc caatgcggag gcagtgcccg
//
LOCUS       DASTKM010000154         4100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1096559, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000154 DASTKM010000000
VERSION     DASTKM010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096559"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFQ10_06465"
     CDS             <1..880
                     /locus_tag="VFQ10_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9965020.1"
                     /translation="GSRRVSAMEIREGEVVGIDLIGRIDALEVGERSLAFWGLGQVGV
                     AIKGPTGVLYVDPYLTDSDGEGGSLPRTFPPPLAPAEVTNASAVLLTHVHVDHTDPDT
                     VMPLSEASPEARFVAPFTSNDTLVEAGLDEKRILVPEIGEPVEIAGSRITAVPSAHTE
                     LERDPKRGYPYLGYVIEWNNVTVYHAGDTVVYDGLIETLSAWDIDVAFVPINGRDYFR
                     TGRGIIGNTDLRETAELTETLDIGLIVPTHYDLIEGNTVDPGHFVSHLYGLNPMRPQK
                     LLRPGELLYFVKDPEG"
     gene            complement(877..1785)
                     /locus_tag="VFQ10_06470"
     CDS             complement(877..1785)
                     /locus_tag="VFQ10_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9965021.1"
                     /translation="MEILPGIHRIESDLGGRFMCQYLLVGEERTVLVDTGLAGTPEEV
                     IVPYLGEIGLGVEDVDEVIISHADVDHCGGNRALKEMNPELRFSCGEADRAWVESTGR
                     IMAEIYLWSEPYGFGPDEESRGWIRTELGGDSPVDVGLRGGETLRLGPDWRVEFLHVP
                     GHTPGHLGIWDAKNDAAIIIDAVLEKGIYDREGNLLQPPRYFDATAYQNTIRNLRSMH
                     PGHLLTAHYQPMRGKEALDFLDRSLDFTMQVHRVVGEGLENGMTDLWTLTKHADAELG
                     PYPAFMVELGAGVRTHLGLLGPNGLR"
     gene            1898..2710
                     /locus_tag="VFQ10_06475"
     CDS             1898..2710
                     /locus_tag="VFQ10_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9965022.1"
                     /translation="MYLSHHQTATGPRWALDEHYLPQDFTLELLLDMPGADVRSFLED
                     LDTEGPAEDALLPPIEQAHEVWASGVTYLQSREARELESTDADAYQKVYDAERPELFF
                     KSLGWRVVGHGNMVRVRGDSHWNVPEPELVLVVNSGMEIVGYTAGNDVSSRDIEGENT
                     LYLPQAKVYDGACSLGPGIVISDPDSMRDLRITMSIRRGDAIVFEDEINTSQMKRSPE
                     ELAAYLGRELAFPGGAFLMTGTGIVPEDDFTLVPGDLVGISVGDLVLENEVA"
     gene            2779..>4100
                     /locus_tag="VFQ10_06480"
     CDS             2779..>4100
                     /locus_tag="VFQ10_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9965023.1"
                     /translation="MIESQIESLNFIGGEWVPADSGETSEIVNPSNPSEVLGTTPRSD
                     KAETERAIEAAAEALPDWKDTLAPARGAVLMEAANIIESREDELARLMALEAGKPMKE
                     ARMEVGRAASIFRYYGSEGWRMPGIAPPSARPGVTHSSIREPLGVVGLITPWNFPLAI
                     PSWKMAPALICGNTIVIKPASNASLNAAALVRILGEAGLPDGVLNMVTGPGGAVGDAL
                     VTDPRVKGISFTGSTSVGLGIQGRATGKKVQLEMGGKNPYVVMEDADLSDAAAKISFG
                     AFGYAGEKCTASSRAIVVEEVYDEFIEELKSATEAMKVGDPLEEDTDIGPVVNKSQYD
                     SILEALETAKNEGRVVLDGGATGSEDDGYYIAPAIVADVDNRSETAQEEIFGPVLAVI
                     KASDFDDAVGLANDTRYGLTAGIATKSLRYAHEFMQRSETGLVNVNLPT"
BASE COUNT          790 a         1296 c         1316 g          698 t
ORIGIN      
        1 cggttcgagg agagtttcgg cgatggagat tcgagaaggg gaggtggtcg ggatcgacct
       61 gatcggcagg atcgacgcgc tggaggtagg agaacggtcg ctagccttct gggggctcgg
      121 gcaggtgggg gtagcgatca agggtcctac cggcgtcttg tacgtcgacc cctacctcac
      181 cgactccgac ggcgaaggcg gcagcctacc gcgcaccttc cccccaccgc tcgcccccgc
      241 cgaggtcacc aatgcctcgg ccgtactctt gacccatgtc cacgtcgacc acaccgatcc
      301 ggacaccgta atgccgctct ccgaggcttc gcctgaggca cgcttcgtcg cgcctttcac
      361 cagcaacgac accctcgtcg aggcggggct ggatgagaag cgcatcctcg tgcccgagat
      421 cggggagccg gtagaaatcg cggggtccag gatcacggcc gtcccttcgg cccacacgga
      481 gttggagcgc gacccaaagc gcggctaccc ttatctgggc tacgtgatcg agtggaacaa
      541 tgttactgtc taccacgccg gcgacaccgt ggtctacgac ggcctgatcg aaaccctctc
      601 cgcctgggac atagacgtgg ccttcgtgcc gatcaacggc cgcgactact tcagaacagg
      661 gaggggcatc atcgggaaca ccgacctccg cgagaccgcg gagctcaccg agacgctgga
      721 tatcggtctg atcgtgccaa cgcactacga cctgatcgaa ggcaacactg tcgacccagg
      781 acatttcgtc agccacctct acggcctgaa cccgatgcgt ccgcaaaagc tgctccgtcc
      841 cggcgagctc ctctacttcg tcaaagatcc ggagggctag cgcaacccgt ttggtccaag
      901 cagcccaaga tgcgtccgga cgcccgctcc tagttcgacc atgaacgccg ggtacgggcc
      961 gagctccgcg tcggcgtgct tcgtgagcgt ccaaagatct gtcatcccgt tctccaaccc
     1021 ttcgccaacc acgcggtgga cttgcatggt gaagtcgagt gagcggtcga ggaagtcgag
     1081 ggcctctttg cctcgcatgg gctggtagtg ggctgtgagg agatggcccg gatgcatgga
     1141 gcggagattg cggatggtgt tctggtaggc ggtggcgtcg aagtagcgcg gcggctgcag
     1201 gaggttgcct tcacggtcgt agataccttt ctcgagcact gcgtctatga tgatggccgc
     1261 gtcgttcttc gcatcccaga tgccaaggtg gcccggcgtg tggccgggaa cgtgcaggaa
     1321 ctcgacccgc cagtctggcc ccagccgcaa ggtctcgccg ccgcgcaggc ccacgtccac
     1381 aggggagtcg ccgcctagct ccgtgcgaat ccagcctctc gactcttcgt cggggccaaa
     1441 gccgtagggc tctgaccaga ggtagatctc cgccattatc cgcccggtgc tctccaccca
     1501 cgcccggtcg gcctcgccac acgagaacct gagctccggg ttcatctcct tgagggcacg
     1561 gttgccgccg caatggtcga cgtcggcgtg gcttatgatc acctcgtcca cgtcctcgac
     1621 gccgagcccg atctccccca ggtaggggac gatgacctcc tcgggcgtcc ctgcgaggcc
     1681 cgtgtcgacg aggaccgttc tctcctcccc gacgagcagg tactggcaca tgaaccgccc
     1741 cccgaggtcc gattcgatgc ggtgtatccc cggcagtatc tccatcccat cctcctcgaa
     1801 agccactggt ggcaccacat taccaccacc cgtgttccgc gcaacgacgc ggagatcgtg
     1861 ttaggttgtg tttgcgagga gaggaggatt gaccaccatg tatctatccc atcatcagac
     1921 cgctaccggc cctcgctggg cgctggacga acactacctt ccgcaggact tcacgctgga
     1981 gctgctgctc gatatgcctg gcgcggatgt ccgaagcttc ctggaggatc tggataccga
     2041 agggccggcc gaagacgcgc tcctgccgcc gatcgagcag gcacacgagg tctgggccag
     2101 cggggtgacc tacctgcaga gcagggaggc ccgcgagctg gagtcgacgg acgccgacgc
     2161 ctatcagaaa gtctacgacg ccgagcggcc cgagcttttc ttcaagtctt tagggtggcg
     2221 ggtcgtgggc cacggcaaca tggtacgcgt ccgcggggac agccattgga acgtgcccga
     2281 gcccgagcta gtcctggtcg tcaacagtgg gatggagatc gtcggctaca ccgcgggcaa
     2341 cgacgtgtcc tcgcgggaca tagaaggcga gaacacgctg tatcttccgc aggccaaggt
     2401 ctacgacggc gcatgctctc tggggccagg catcgtgatc tccgatcctg attcgatgcg
     2461 ggacctccgc atcaccatga gcattcggcg cggcgacgcc atcgtcttcg aggacgagat
     2521 aaacacttca cagatgaagc gctcccccga agaacttgcg gcctatctcg gcagggagct
     2581 agcctttccc ggcggggcct tcctgatgac cggcacgggg atcgtacccg aagacgattt
     2641 cacgctagtg cccggggacc tggtcgggat ctcggtcggg gatctcgtgc tggagaacga
     2701 ggtggcgtag gtcggctcat agaatgggcg agaatcgggt agagagaaga ggtaggcgtt
     2761 ttatctagga ggaagacctt gatcgaatcg cagatagaat ccctgaactt tatcggcggc
     2821 gagtgggtcc ccgcagactc gggcgagacc tcggagatag tcaatccctc gaacccctcc
     2881 gaagtcctcg ggaccacccc cagatcggac aaggcggaga cggagcgggc catagaggcc
     2941 gcggccgaag ccctgcccga ttggaaggac acgctggccc ctgcccgcgg cgcagtcctg
     3001 atggaggccg caaacattat cgagtcgcgc gaggacgagc tggcgcggct catggcgctg
     3061 gaggccggca agccgatgaa ggaagcccgc atggaggttg gacgcgcggc gagcatcttc
     3121 cgctactacg gctccgaagg ctggcgcatg ccaggcatag ccccgccctc cgcgcgtccc
     3181 ggcgtgaccc acagttccat aagggagccc ctgggggtcg tggggctcat aacgccgtgg
     3241 aacttcccgc tggcgatacc gtcgtggaag atggcgccgg ccctgatctg tggcaacacc
     3301 atcgtgataa agccagcgag caacgcctcc ctgaacgcgg ccgcactggt gcgtatcctg
     3361 ggcgaggcag gcctacccga cggggtcttg aacatggtaa cgggtcccgg cggagcggta
     3421 ggcgacgctc tcgttaccga cccgcgcgtc aagggtatct cgttcacagg ctcgacgagc
     3481 gtagggctcg gtatacaagg ccgggccaca gggaagaagg tccagttgga gatgggcggc
     3541 aagaaccctt acgtcgtgat ggaggacgcc gacctctcag acgcggcggc caagatctcc
     3601 ttcggcgcgt ttggatacgc cggcgagaaa tgtacggcct cgagccgggc catcgtcgtc
     3661 gaggaggtct acgacgagtt tatcgaggag ctcaagagcg cgaccgaggc catgaaggtc
     3721 ggcgacccgc tcgaagaaga tacggacata ggacccgtcg tcaacaagag ccagtacgac
     3781 agtattctcg aagccctgga gacggccaag aacgagggac gagtggtact cgatggcgga
     3841 gccactggtt ctgaggacga tggctattac atcgcccctg cgatcgtcgc cgacgtagac
     3901 aacaggtcgg agaccgcgca ggaagagatc ttcggtcccg tcctcgccgt cataaaggcc
     3961 agcgacttcg acgacgccgt cgggctcgcc aacgacaccc gctacgggct gaccgcgggg
     4021 atagcaacca agtctctgcg ctacgcgcac gagttcatgc aacggtcgga gacggggctc
     4081 gtgaacgtca acctcccgac
//
LOCUS       DASTKM010000155         7392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_920648, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000155 DASTKM010000000
VERSION     DASTKM010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920648"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1626
                     /locus_tag="VFQ10_06485"
     CDS             <1..1626
                     /locus_tag="VFQ10_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET9965024.1"
                     /translation="KLPPLERLEVRVSEEPVALSFEPVGASPLGDESGRWCVAVSVDE
                     VRVAESLPDVPVKVHDSKSLGVRGADFDTYLAAYLVKPGLGSYELETLAAERGMAEVE
                     VSYEDRTVAEAARRAALVYALSPRLEEELEAMGLARLYYDVELPLAGVLGEIEEIGMP
                     VDAGTLEEVGEELEGRISELERHIYEEVGHSFNIGSPKQLGEVLFEEMGIPPVRKTKT
                     GYSTDAKVLQQLAIEHPVADRIIEYRELTKLMGTYIEGLLKLVAEDGRIHSTLHQTRA
                     ATGRLSSDSPNLQNIPIRTEMGVRIRDAFTASPGRRLVVADYSQIELRILAHMTGEQA
                     LVESFMKGEDIHTRTASEVFDVRMESVTPELRRRAKMVNFGILYGISGFGLATRLGNV
                     HPAEAERYIKRYFERYPRVTEFMQETLKEAEELGYSTTLFGRRRYVPELRNANKNVRK
                     LGERIAFNARVQGTAADIIKVAMVDLAPRLGPLGADMIMQVHDELVFDVDEDRVEEVA
                     GLAVDRMVAAYDLKPPLEVEAKVGDLWGRVSPL"
     gene            1750..2967
                     /gene="rpsA"
                     /locus_tag="VFQ10_06490"
     CDS             1750..2967
                     /gene="rpsA"
                     /locus_tag="VFQ10_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET9965025.1"
                     /translation="MKDFFREENGEVVPVDESVLIDFKDGDIVEGEVVRIDRDEVLVD
                     IGYKSEGLVPSNELTIRKGADPHDVVELGQRLEALVMQKEDADGRLILSAKRAAFERA
                     WNRIEDAYNDQRTVEGPVIEVVKGGLILDIGLRGFLPASLVDIRRVRNLDSFLGQRLE
                     CKVIELNRSRNNVVLSRRAVLEEERKEERERILTSLEEGMIVRGTVSNLVDFGAFVDL
                     EGIDGLIHISELSWQHVDHPSEVVEVGEEVEVKVLEVDRDRERISLGLKQTRKDPWQE
                     IVERVKIGEQIHGRVTKLVSFGAFVEVADGVEGLIHISELADHHVETPDEIVRSGDEV
                     DARIIDVDARRRRLSLSLRPKREEREERPRREERPRREPEQRGTSDRRSGEGSGLRSG
                     AFDALADLDLEER"
     gene            3006..3611
                     /gene="coaE"
                     /locus_tag="VFQ10_06495"
     CDS             3006..3611
                     /gene="coaE"
                     /locus_tag="VFQ10_06495"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET9965026.1"
                     /translation="MTVAVTGPFASGKSTLVRFLGELPGTETASADEIVHHLLKNDHR
                     TISRIIERFGEGVRGPHGIDRKALGREVFGDAGALRDLEEILHPLVRSETDRRIEASR
                     VQVFVVEIPLLFETGRGGDFDFTVAVTVPEERRRAWAAERGLDEAALLAVEARQLSGE
                     EKARRADLVVQNDGDLDRLGERAHELGRMILRERGSHGNPQ"
     gene            3670..4200
                     /locus_tag="VFQ10_06500"
     CDS             3670..4200
                     /locus_tag="VFQ10_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9965027.1"
                     /translation="MVFGVLAILPFALRLPDAVMRTMYPLRYEAVIRQASEENDLDPT
                     FVAGVIYTESRFRPDAESQSEAYGLMQLLPQSARFIQHKSGIKGDYRDPEVNIRLGTW
                     FLGYLEDRYNGDERHMLAAYNSGEGSVDAWISDKDFDIAKDIPYKETREYVDKVLQAQ
                     KTYQELYGKNLQHNPG"
     gene            4366..6369
                     /gene="uvrB"
                     /locus_tag="VFQ10_06505"
     CDS             4366..6369
                     /gene="uvrB"
                     /locus_tag="VFQ10_06505"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET9965028.1"
                     /translation="MERSTRTGFRVNSEYQPTGDQPRAIEQLASGLDAGMKAQTLLGV
                     TGSGKTHTMARVIEEAGSPALVIAHNKTLAAQLASEFAEFFPNNAVEYFVSYYDYYQP
                     EAYVPRTDTFIEKDAQINEDIDRLRHSATSSLFTRRDVIVVASVSAIYGLGSPEEYRS
                     KMVLLERGGFYDLDDVLRDLVKIQYTRNDFQLNRGNFRVRGDVLEVHPAYQDTIYRIS
                     FFGDEVENMTEVDPLTGEILREHEILTVYPASHFVTGEDRLATAVKNIEAELEGRLGE
                     LEGEGKVLEAYRLKQRTQYDLEMMRELGYTSGIENYSRHIDGRPPGSTPYTLLDYFPG
                     DYITFIDESHITLPQVRGMYNGDRSRKTTLVEFGFRLPSALDNRPLMWDEFLLKTNQM
                     VFVSATPGPYELENSGQIVEQIIRPTGLVDPEVEVRPTKNQIDDLLNEVAKRVEHGER
                     VLITTLTIKMAEDLTDYLLEHNVKARYMHSNIETLDRIQIIRGLRTGEFDVLVGINLL
                     REGLDLPEVTLVAILDADKEGFLRGERALIQTIGRAARNVNGRVIMYADKETDAMRVA
                     IGETERRREIQNSYNEQHGVEPRTITKGVSDILIAAEAKGLYKAGKKSGREAPRDPDE
                     LRTLIADLEAEMLEAAEELKFEYAAKLRDEIKDLEKQLQEVAS"
     gene            complement(6380..7195)
                     /locus_tag="VFQ10_06510"
     CDS             complement(6380..7195)
                     /locus_tag="VFQ10_06510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9965029.1"
                     /translation="MRRFASRRPFAFSVLVTLALFGLDLASRAVFPRAPVGNIEKLPQ
                     DAFEPPVGLGLILSDMRSPDTLFWALATVLALGLLVWTGWLREAGFNRMSQWRNLRLL
                     LFPLLVCALTLSGGLFGSGPASLVSAFLTALVAAFGEEIVFRGLLWRALAPAGPVRAV
                     ILTSLLSGLLVLGRTATDGPWPEAVRLTALTLCGGFTYGALRWRTTSIWPVIVVHTAL
                     AFAVSIATLGAVTYPLLMLLSTLGFVAYGLYLLRNPQVRADGGLTKPAPSRVR"
BASE COUNT         1571 a         2198 c         2439 g         1184 t
ORIGIN      
        1 aaactcccgc cgctcgaacg tctggaggtg cgtgtctccg aggagcccgt cgcgttgtcc
       61 ttcgaacccg tgggagcgtc cccgctcggg gacgagagcg ggcgctggtg cgtcgccgtc
      121 tcagtggacg aagtgcgcgt cgctgagagt ctgcctgacg tccccgtcaa ggtgcatgac
      181 tccaagagcc tcggggtgcg tggggcggat ttcgacacct atctcgccgc gtacctggtc
      241 aagcccgggc tggggagcta cgagctggag acgctagcag cggagagggg gatggccgag
      301 gtcgaagtca gctacgagga caggacggtc gccgaggccg ccaggagggc cgcgctggtg
      361 tacgcgctct cgccgaggct cgaggaggag ttagaggcga tggggctcgc caggctctac
      421 tacgacgtgg agctcccgct cgccggcgtg ctcggggaga tagaggagat cgggatgccc
      481 gtcgacgccg gcaccctcga agaggtcgga gaggagctgg agggacgtat ctcggagctg
      541 gagcgacata tctacgagga ggttggacac tccttcaaca tcgggtcgcc caagcaactc
      601 ggcgaggtcc tcttcgagga gatgggcata ccacctgtca gaaagaccaa gacaggctac
      661 tccaccgatg ccaaggtatt gcagcagttg gccatagagc atcctgtggc cgaccgcata
      721 atcgagtacc gcgagctgac caagctcatg ggcacgtaca tagagggttt gttgaagctc
      781 gtcgccgaag acggccgcat acacagcacc ctgcaccaga cgcgggcagc cacgggtcgc
      841 ctttcgagcg actcgcctaa tttgcagaac atcccgatcc gcacagagat gggcgtgcgg
      901 atcagggacg cgtttaccgc gtcgccgggg cgcagactgg tggtcgccga ctactcccag
      961 atagagctca ggatcctggc ccacatgacc ggagagcagg cgctcgtcga gtcgttcatg
     1021 aagggcgagg acatacacac gcgcactgca tcagaggtct tcgacgtgcg catggagagc
     1081 gtgacgccag agctcaggcg ccgggcgaag atggtcaact tcggtatcct ttacggcatt
     1141 tcggggttcg ggctcgcgac gaggctaggg aacgtgcatc cggccgaggc cgagcggtac
     1201 atcaaacgct acttcgagcg ctacccgagg gtcacggagt tcatgcagga gaccctgaag
     1261 gaggccgagg agctggggta ctccacgacg ctcttcggga ggcggcgtta cgtgcccgaa
     1321 ctgaggaacg ccaacaagaa cgtgaggaag ctcggggaga ggatcgcctt caacgcccgc
     1381 gtccagggca cggcagcgga tattatcaag gtggcgatgg tcgacctcgc gccgaggttg
     1441 ggtcctctcg gggcggatat gatcatgcag gtccacgacg agctcgtctt cgacgtcgac
     1501 gaggaccgtg tcgaggaggt cgccggcctc gccgtagata ggatggtcgc ggcctacgac
     1561 ctcaagccgc cgctcgaagt cgaggccaag gtcggcgatc tttgggggcg ggtgagccct
     1621 ctgtagaccc catctcaggc ccggttttcg cgggcagaat tgctgtataa tctgcacccg
     1681 tcaagcgaag taaaggaaag caactgtttc atgactgaca ctacacagaa gccgtcccgg
     1741 gaattgacca tgaaagattt cttccgggag gagaacgggg aggtcgtccc cgtcgacgag
     1801 agcgtcctca tagatttcaa agacggggac atcgtcgagg gcgaggtcgt ccggatagac
     1861 agggacgagg tactcgtcga catcggctac aagtcggagg ggctcgttcc ctccaacgaa
     1921 cttaccatcc gcaagggcgc agacccgcac gacgtagtcg agctcggcca gcgcctcgaa
     1981 gccctggtca tgcagaagga ggacgccgac ggacggctca tcctctcggc caagcgggcg
     2041 gctttcgagc gggcctggaa ccgcatcgag gatgcctaca acgaccagcg caccgtcgag
     2101 ggacccgtca tcgaggtggt gaagggtggc ctgatcctcg acatcgggct gcggggcttc
     2161 ctgcctgcga gcctggtgga tatcaggcgc gtaaggaatc tcgactcgtt cctcggccag
     2221 aggctggagt gcaaggtcat cgagttgaac cggagccgca acaacgtagt tctgtccagg
     2281 cgcgccgtgc tcgaggagga gcgcaaggag gagcgcgagc gtatcctgac ctcgctggaa
     2341 gagggcatga tcgtcagggg caccgtctcc aacctcgtgg atttcggggc cttcgtggat
     2401 ctcgaaggca tcgacgggct gatccacatc tccgagcttt cttggcagca cgtcgaccat
     2461 ccgagcgagg tcgtcgaggt gggcgaggag gtcgaggtca aggtcctcga ggtcgaccgc
     2521 gaccgcgagc gcatctcgct tggtctcaag cagaccagga aggacccgtg gcaggagata
     2581 gtcgagcggg tcaagattgg agagcagatc cacggaaggg ttacaaagct cgtctcattc
     2641 ggggccttcg tggaggtcgc agacggggtg gagggtttga tccacatctc cgagctggcc
     2701 gaccatcacg tggagacgcc agacgagatc gtgcgcagcg gcgatgaggt cgacgcccgt
     2761 atcatcgacg tcgacgcccg ccgccgcagg ctctccctga gcctgcggcc gaagcgcgag
     2821 gagcgcgaag agcgtccccg ccgcgaggag cgtccgcgcc gtgagcccga gcagcgcggc
     2881 acctcggacc gcagaagcgg cgagggtagt ggcctccgct ccggcgcctt cgacgcgctg
     2941 gccgacctgg acctcgagga gagatagaca aggactcttt acacagaggg tccgtggaag
     3001 ggccggtgac cgtagccgtc accggccctt tcgcgtcggg caagagtacg ctggtccgct
     3061 ttttgggtga actgcctggc acggagacgg cctcggccga tgagatcgtg caccacctcc
     3121 tgaagaacga ccaccggacg atttccagga tcatcgaacg cttcggggaa ggtgtaagag
     3181 gaccgcacgg tatcgaccgc aaagccctcg gccgcgaggt attcggtgac gccggggcac
     3241 tgcgggacct ggaagagata ctgcatcctc tggtacgcag cgagaccgac aggcgaatcg
     3301 aggcctcgag ggtgcaggtg ttcgttgtgg agataccgct gctcttcgag acgggccggg
     3361 ggggggactt cgacttcacg gtggccgtca cggtgccgga agagaggcgc cgtgcgtggg
     3421 ctgcggagcg gggcttggac gaggcggcgc tgctggccgt ggaggcacgg cagctctcgg
     3481 gtgaggagaa ggccaggcgt gccgacctgg tggtacaaaa tgatggagac ctggatagac
     3541 ttggcgagcg ggcgcacgag ctcgggcgca tgatcttgcg ggagagagga tcccacggca
     3601 acccacagta agagacgcag gtacggaagg tcgcgggccc gccgccggcg cgggattctc
     3661 gtgttgctgg tggtttttgg ggtcctcgcc atcctgcctt tcgccctcag attacccgat
     3721 gccgtgatgc ggaccatgta ccccctacgg tatgaagcag tcatccgtca ggccagcgag
     3781 gagaacgacc tcgatcccac attcgtggct ggcgtaatct atacggagag ccgcttcagg
     3841 ccagacgccg agtcccaaag tgaggcctac gggctcatgc agctacttcc acaatcagcg
     3901 cgcttcatcc agcacaagag cggcataaag ggcgactacc gcgacccgga ggtcaacata
     3961 agattgggaa cctggttcct cggttacctc gaggatcgat acaacggtga tgagaggcac
     4021 atgctcgcgg cgtacaactc aggtgaaggg tcggttgacg cgtggatctc ggacaaggac
     4081 ttcgacatcg cgaaggacat accttacaaa gagacccgcg agtatgtgga caaagtgctc
     4141 caggcacaaa agacctacca ggaactctac ggcaagaacc tccaacataa cccagggtag
     4201 aaagcgggaa tccaccggca cgtgcgttcc acgctcccat gcttgcgctt cgcatgatat
     4261 cggcgccgaa aagaccgaga aaaagccagt gttcgcagta taatcggatc gtatgttcca
     4321 gaagcgcgac gcttcacgac ccacgagaga tcccgaggtt tttgtatgga gagatcgacg
     4381 cggaccgggt tccgggtaaa cagcgaatac cagccgaccg gcgaccagcc gcgggccatt
     4441 gagcagctcg cctctggtct cgacgctggc atgaaggcgc agaccttgct gggcgttacg
     4501 ggatctggga agacccacac catggcccgg gttatagagg aggccggtag tccggcgctc
     4561 gtcatcgcgc acaacaagac gctcgcggcg cagctggcca gcgagttcgc cgagttcttc
     4621 cccaacaacg ccgtcgagta cttcgtcagc tactacgact actaccagcc cgaggcttac
     4681 gtcccgcgca cggacacctt tatcgagaag gacgcccaga tcaacgagga catcgaccgt
     4741 ctcaggcact cggcgacgag ctctctgttc acccgcaggg atgtgatcgt ggtggctagc
     4801 gtctcggcca tctatggcct cggtagcccc gaggagtacc gctcgaagat ggtccttctg
     4861 gagcggggtg gtttctacga tctcgacgat gttttgaggg acctggtcaa gatccagtac
     4921 accagaaatg acttccagct caacaggggg aacttcaggg tgcgcggcga cgtgctcgag
     4981 gtgcatcccg cttaccagga tacgatctac cgcatctctt tcttcgggga cgaggtcgag
     5041 aacatgaccg aggtcgaccc tcttacgggt gagatcctgc gcgagcacga gatcctgacc
     5101 gtctaccccg cgtcccactt cgtcacgggc gaagacaggc tcgcgaccgc tgtcaagaac
     5161 atcgaggcgg agctcgaggg gcggctggga gagctggaag gcgagggcaa ggtcctggag
     5221 gcgtaccgcc tcaagcagcg cacccagtac gacctggaga tgatgcgcga gctcggctac
     5281 acctcgggca tcgagaacta ctcgcgccac atcgacgggc gtcctcctgg ctccacgccg
     5341 tatacgcttc tggattactt ccccggagac tacatcacgt tcatcgacga gtcgcacata
     5401 accctgcccc aggtcagagg catgtacaac ggcgacagga gcaggaagac caccctggtc
     5461 gagttcggct tcaggctacc gagcgccctg gacaaccgtc ctttgatgtg ggatgagttc
     5521 ttgctcaaga cgaaccagat ggtcttcgtc tcggcgacac cagggcctta cgagctcgag
     5581 aactcggggc agatagtgga gcagatcatc cgcccgacag gcctcgtcga ccccgaggtc
     5641 gaggtgcgtc ccacaaagaa ccagatcgac gacctcctca acgaggtagc caagcgcgtc
     5701 gagcacggcg agcgagtgct catcaccacc cttaccataa agatggccga ggacctcacg
     5761 gactacctgc tcgagcacaa cgtcaaggcg cgctatatgc actccaacat cgagacgctg
     5821 gacaggatcc agatcatccg cggccttcgt acaggagagt tcgacgtgct cgtcggcatc
     5881 aacctcctcc gcgagggcct cgacctcccc gaagtaacgc tcgtcgccat cctcgatgcg
     5941 gacaaagagg gcttcttgcg cggtgagcgg gcacttatcc agaccattgg ccgcgccgcc
     6001 cgcaacgtca acgggcgggt catcatgtac gcagataagg agaccgacgc gatgcgcgtc
     6061 gctattggag agacagaacg cagacgtgag atccagaact cttacaacga gcagcacggc
     6121 gtcgagccgc gcaccataac caagggcgtc tccgacatcc tcatcgccgc cgaggccaaa
     6181 ggtctgtaca aagcaggcaa gaagagcggc cgtgaagccc cacgcgaccc cgacgagcta
     6241 cgcaccctga tcgccgacct cgaagccgag atgctcgaag ccgcagaaga gctcaagttc
     6301 gagtacgccg ccaaactccg cgatgagatc aaagacctcg aaaaacaact gcaagaggta
     6361 gcctcgtagg acgaggctct cacctcacgc gtgatggcgc gggcttcgtc aggcctccgt
     6421 ccgccctgac ctgggggttg cggaggaggt agagcccgta ggctacgaaa ccaagggtgc
     6481 tgaggagcat caagagggga taggtgacgg cgcccagagt cgcgatgctc acggcgaagg
     6541 ccaaggcggt gtggaccacg atcacgggcc agatggaggt ggtgcgccag cgaagcgcgc
     6601 cgtaggtgaa gcctccgcag agggtcagcg ctgtaaggcg gacagcttcg ggccaggggc
     6661 cgtccgttgc ggttctgcct aggacgagca ggccagagag taaggaagtc aggatcacag
     6721 cccgtaccgg acctgccggc gctagcgccc gccacaacag gccgcggaag actatctcct
     6781 cgccgaaggc ggcgacgagc gcggtgagga acgccgagac cagcgacgct ggccccgatc
     6841 cgaagagtcc gccggagagg gtcagcgcgc agacgagtag cgggaaaagg agtaggcgta
     6901 ggttcctcca ctgtgacatc cggttgaagc cggcctcacg caaccagccg gtccacacga
     6961 gcaggccgag ggccagtacc gtcgcaagcg cccagaacag ggtatcagga ctcctcatgt
     7021 cggagaggat caggccaaga cccacgggag gctcgaaagc atcctgcggg agcttctcga
     7081 tgttgcctac cggcgccctc ggaaaaacgg ccctgctggc gagatctagc ccgaacaaag
     7141 ccagcgtgac aagcacggag aaggcgaagg ggcggcggct tgcgaaacgt ctcacaagcc
     7201 cgctcctgta tatccggtga gcgttatcag agcctatcct tgaggccggc aaggatgagg
     7261 tcgaggccga actcgaactc cgcgtcgtga tccacttcgc gcagaggtgc gtcgagctcg
     7321 tggatgtgcg ggaagtcgtc tacagagagc gtgccggcgc cggcacgggc gcccgcgggc
     7381 tccatcgcaa aa
//
LOCUS       DASTKM010000156         3344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1449197, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000156 DASTKM010000000
VERSION     DASTKM010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449197"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(513..1040)
                     /locus_tag="VFQ10_06515"
     CDS             complement(513..1040)
                     /locus_tag="VFQ10_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET9965030.1"
                     /translation="MTLNASYEPVALVTVKRAIVLVVSEKAEIVEANLDRRFRSERAE
                     YPYPLVIRLVKYVEIPRRLRKHVTNAILFARDNYRCQYCSRHRNALGRRDRLTRDHVK
                     PISRGGANTWDNVVTACTKCNARKGNRLPMECGMYPKSTPKEPRYVVMVWYSRDLNEV
                     QRRYVDQFYGGLTAS"
     gene            complement(1176..2225)
                     /locus_tag="VFQ10_06520"
     CDS             complement(1176..2225)
                     /locus_tag="VFQ10_06520"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HET9965031.1"
                     /translation="MIRGTAPERRYAILAEGRFADRSAKTAHGLIRYGKDEVVCVMDS
                     TLAGRRVADVLPDLGRDAPIVGTLEEALELSPTSVLVGLAPPGGRLPEEWMETLRVAA
                     EAGLEIVSGLHQRLAPEFPGKPVWDVREPPEGISLFSGKGFEVGPKVALTVGTDSAIG
                     KMTATLEIERAAREAGLSTEFVPTGQTGVVIAGWGICVDAVVSDFVAGASERLVLEAA
                     RTSPDLILVEGQGSLGHPAYSGVTLGLMHGSCPDCLILCSADPHEEVFRDVPRPSPAR
                     IARLYEEVASLVKPAPVVAVSLNTRGLDEERARELIAAVSDETGLPAADPFRGSAAPI
                     LEAVLEASKSRAVGL"
     gene            complement(2222..3217)
                     /locus_tag="VFQ10_06525"
     CDS             complement(2222..3217)
                     /locus_tag="VFQ10_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HET9965032.1"
                     /translation="METSVTTVKVHTERTFAIARSSADTFERVIFEISEDGVTGRGEA
                     APTNYYGQDAQGVAAALEGVEAGDPWDIEGALARNYAIPPSALTALDNAMHDLAARRL
                     GVPVYRLLGLARPEPVSAYTLGIADRETTVAHAQSLRAHRILKVKVGGWNDIETLRTV
                     RESSEADLWVDANEAFSPEEAVEVVTELKEIGVGMIEQPVPVFAGTEALRTVTEAAFP
                     VPLIADESAITARDVPGLAGCVSGVNVKLAKCGGIRRALEMIHTARAHGMLVMLGCMV
                     ETSLGISAAAQISGLVDFVDLDGAMLLADDPYAGPVYDNGRILLPEEPGLGVEPR"
BASE COUNT          583 a         1084 c         1061 g          616 t
ORIGIN      
        1 ggtgcgcgtt gtgtaccacc cgcaacccgg tcaagaagaa tacgaaggaa catcccagag
       61 cgggcaagaa cagcaccggc catgaccacg ccaaccccgc aagtgacagc gacgctccaa
      121 accacggcac cgggagacac agctccctga ccacctccca ccggctcaac gtaaccaggt
      181 cccgctactc gatgccccga acgcgcggat cacgcataat ctccaggtta acaggtgtca
      241 cctccgatca ggtccccgcg tctcttttgt tcaagtttga tctattttgt gtgattaagt
      301 aacggttgtc cgtgaacatt gttacaaaat tccgcccctg gcgatccgta gaacgatgcg
      361 agtcagaact tcagcaagtc agcctccgtt ctggtcagcc gtggctttga acgagcaatg
      421 cttcccagaa acggttcgtc tggcgctttt ggtaaagtac cgctaggctc tcgccaacgg
      481 ccgtcgaaga cggcgtgctg aagtgctgac agctaggagg ccgtcaggcc tccgtagaac
      541 tgatctacgt accggcgctg gacttcgttg aggtcgcgag agtaccacac catgactacg
      601 taacgtggtt ctttgggcgt gctcttgggg tacatgccgc attccatcgg caggcgattg
      661 cccttcctcg cgttgcactt ggtgcatgcg gtcacgacgt tgtcccaggt gttcgcgccg
      721 ccgcgcgaga tcggcttgac gtggtcgcgg gtcagccggt cgcgccgccc gagggcgttc
      781 cggtgcctcg agcagtactg gcagcggtag ttatcgcggg cgaagaggat cgcgttggtc
      841 acgtgcttgc gcagacggcg gggtatctcg acgtacttga cgagacggat cacaagcggg
      901 taaggatact cggccctctc ggagcggaac cggcggtcta ggttcgcctc gacgatctcg
      961 gccttttcgg agacgacgag gactatagcc ctcttgaccg tcaccagggc cacgggctca
     1021 tagctcgcgt tgagcgtcaa gcatcgtgcc acgccgagat attaacataa gctagccctt
     1081 cagcatgtca gcacgatccg cccttcgggc atcgcttgcc agcatttcag tttgtcagct
     1141 tctcgttttc gaggatcggt tgtagcggga tgcccttaga gaccaaccgc ccggctcttg
     1201 gatgcctcga gcacggcttc gaggataggt gcggcggaac ctctgaaggg atccgcggcg
     1261 gggagtccag tctcgtcgga tacggcggcg atgagttctc gggctctttc ttcgtccaac
     1321 cccctggtgt tgaggctaac ggcgacgacg ggcgcaggct tgaccaggga tgcgacctcc
     1381 tcgtacaggc gggctatgcg ggctggggaa gggcggggga cgtctctgaa gacctcctcg
     1441 tgcgggtcgg cggagcacag gatcaggcag tcggggcaag agccgtgcat gaggccgagc
     1501 gtcacgcccg agtacgccgg atgcccgagg gagccctggc cctccacgag gatgaggtcg
     1561 ggcgaggtcc tggccgcctc gagcaccagc cgttcggagg caccagcgac gaaatccgag
     1621 accacggcgt cgacgcagat accccacccc gcgatgacta cgccggtctg tcccgtgggg
     1681 acgaactcgg tggagaggcc ggcctccctg gcagcgcgct ctatctccag ggtcgccgtc
     1741 atcttgccga tggcgctgtc cgtccctacg gtgagcgcga ccttgggccc gacctcgaag
     1801 cccttcccgg agaagagcga gatgccctca ggcggctcgc gcacgtccca gacaggcttg
     1861 ccgggaaact cgggcgcgag ccgctggtgg aggccgctga cgatctcgag cccggcttcc
     1921 gccgcaaccc gcaaggtctc catccactcc tctgggagcc tcccgccggg aggggcgagt
     1981 ccgaccagta cagaggtcgg cgagagctcc agcgcctctt cgagcgtccc gactattggc
     2041 gcatcgcgcc cgaggtcggg caagacgtcg gccacgcgcc tccccgcgag cgtcgagtcc
     2101 ataacacaga cgacctcgtc cttgccgtag cggataaggc cgtgcgccgt cttggctgag
     2161 cggtcggcga accggccctc ggccaggata gcgtaccgcc gctccggcgc cgtgccacga
     2221 atcatcgcgg ctccaccccg aggccgggtt cctcgggcag aaggatgcgg ccgttatcgt
     2281 agaccggtcc tgcgtaggga tcgtcggcga gaagcatcgc gccgtcgagg tcgacgaagt
     2341 cgacgaggcc ggatatctgg gccgccgccg agatgccgag ggaggtctcc accatgcagc
     2401 cgagcatgac gagcatcccg tgggcacgag cggtgtggat catctccagc gcgcgccgga
     2461 tcccaccgca cttggcgagc ttgacgttga ccccgctgac acaccccgca aggcctggca
     2521 catccctggc cgtgatggca ctctcgtccg cgatcaacgg taccgggaat gccgcctccg
     2581 tcactgtgcg tagggcttct gtgccggcaa agacagggac gggttgttcg atcataccga
     2641 cacctatctc cttcagctcc gttacgacct ccacggcctc ctcgggggag aaggcctcgt
     2701 tggcgtccac ccagagatcc gcctcggatg actccctcac ggtcctgagc gtctcgatgt
     2761 cgttccatcc tccgaccttt accttcagga ttcgatgcgc ccgcaggctc tgagcatggg
     2821 cgacggtcgt ctccctgtcg gctatgccca gggtgtatgc cgagacgggt tctggcctgg
     2881 cgagcccgag caaacggtag accggcacgc cgagcctcct ggccgcaagg tcgtgcatgg
     2941 cgttgtcgag agccgtcagc gcggacgggg gaatcgcgta gttgcgcgcg agggcgccct
     3001 cgatgtccca ggggtccccg gcctcgacgc cctccaaagc tgccgccacc ccctgtgcgt
     3061 cctggccgta gtagttcgtg ggtgccgcct cgcccctgcc cgtgacgccg tcttccgaaa
     3121 tctcgaagat tacgcgctcg aaggtgtctg ccgagctgcg ggcgatcgcg aaggtgcgct
     3181 cggtgtgcac tttgacggtc gttacgctcg tctccaagtc cgccccctcc tccactctat
     3241 ccggatagca ttgtagcggc gggcgagtcc ttcttccgcc agcaacgttt cgtgttcgaa
     3301 ggtagtatta ggatcaggat ggccgcaaat gggaaacggg ggta
//
LOCUS       DASTKM010000157         4994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_39621, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000157 DASTKM010000000
VERSION     DASTKM010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39621"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VFQ10_06530"
     CDS             <1..1137
                     /locus_tag="VFQ10_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET9965033.1"
                     /translation="GVGGGAAALIALSEFYHLSTEDEMSLVAGITVPLTVLIGSVSFA
                     GSAIAFTKLQELAFTGSVAFPLQQVVNALLFAGVLVLGANALLGGSILPFLSPVASVV
                     AMLAASLVLGILLVMPIGGADMPIVISLLNAFTGLAAAASGFVLENYILIIGGTIVGA
                     SGTILTRLMSSALGRPLGKIIFTGIAASGGGGSEQQEDREVNDTSAGEVGEFLADEAE
                     KVVIVPGYGLAVAQAQQAMRDLVETLESEGKEVLFGIHPVAGRMPGHMNVLLTEVGIS
                     YEKLYDLEDINPEFEDTDAAIIVGANDVVNPSANDPEVESPISGMPVLTVENAEHVIF
                     IKRSLSPGFAGVDNPLFYDTEKTMMLFSDAKQGLQDLNEAVKNP"
     gene            2124..2483
                     /locus_tag="VFQ10_06535"
     CDS             2124..2483
                     /locus_tag="VFQ10_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965034.1"
                     /translation="MNGCHRAGFVLDRPADANQTAQGCQESTRLARQGIFWWLLFCLQ
                     GRLTAFELPGHRRSVESKVLGVGLEGPEHVHGREYFEVLVLQVTEVPGPDLRHLLDLL
                     ERQTSLLTGPLQGLAYL"
     gene            complement(3223..3666)
                     /locus_tag="VFQ10_06540"
     CDS             complement(3223..3666)
                     /locus_tag="VFQ10_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HET9965035.1"
                     /translation="MPIRKAQAAWHGTFRDGEGKMRLGSGAFEGAYSYRSRMEDSDPG
                     TNPEELLGAAHAGCFSMSLARRLSAAGYPPERIHTEARVRFGRAGEGYAISRIELRTE
                     AKVPEIDEELFLEKAEAAKRDCAVSKALAGVEAIDLEAKLVEHAG"
     gene            4084..4296
                     /locus_tag="VFQ10_06545"
     CDS             4084..4296
                     /locus_tag="VFQ10_06545"
                     /inference="COORDINATES: protein motif:HMM:NF022608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HET9965036.1"
                     /translation="MSQDECKQGDKVEWNAPQGKTRGTVKKKLTSSTEVGGQKINASE
                     AKPRYLVESEKSGKEAAHKPGALSKV"
     gene            4382..4561
                     /locus_tag="VFQ10_06550"
     CDS             4382..4561
                     /locus_tag="VFQ10_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965037.1"
                     /translation="MKIHTEDCEVGNVPGPETFTGHEGLREFGTSYRSTYGEMRSEFR
                     AVFYSRPFLLTDESR"
     gene            complement(4558..>4994)
                     /locus_tag="VFQ10_06555"
     CDS             complement(4558..>4994)
                     /locus_tag="VFQ10_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9965038.1"
                     /translation="TRGKLMNERIRAMIEIWTKDEPEFHGEYVDFDPIGIWPKPVQKP
                     YPPIYVGGGSQAFERVAQFGDAWLANGLPPDKLEPMMGELREVAGRDVPVSVFNASSE
                     PEDLKAYAHLGVERVLLGLPTLPESETLKQLDDLAQVATDIR"
BASE COUNT          932 a         1555 c         1530 g          977 t
ORIGIN      
        1 ggcgtcggcg gcggcgcggc ggcgctcatc gcgctctcgg agttctacca cctgagcacg
       61 gaggacgaga tgagcctcgt cgccgggatc acggttccgc tcaccgtcct gatcgggtcg
      121 gtcagcttcg cgggcagcgc catcgcgttc accaagctcc aggagcttgc cttcaccggc
      181 tccgtggcct tcccgctcca acaggtggtc aacgcgctcc tgttcgcggg cgtcctcgtg
      241 ctgggagcta acgctctgtt gggcgggagc atactgccgt tcctctcgcc ggttgcctcg
      301 gtggtcgcca tgctcgctgc ctcgctcgtg ctggggatac tgctcgtcat gcccatcggc
      361 ggggcggaca tgcccatcgt gatctcgctg ttgaatgcgt tcacggggct ggcggcggcg
      421 gcgtccgggt tcgtgctgga gaactacatc ctgatcatcg gcggcaccat cgtcggagcg
      481 tcgggcacaa tactcacgcg cctgatgtcg agcgcactgg ggcgtcctct aggcaagatc
      541 atcttcaccg gcatcgccgc ttccggaggt ggcggaagcg agcagcaaga ggatcgggaa
      601 gtcaacgaca cgagcgccgg ggaggttggc gaattcctcg cggacgaggc ggagaaagtc
      661 gtgatcgtac ccggttacgg cctcgccgtc gcccaggccc agcaggccat gcgagacctc
      721 gtggaaacgc tggagtccga gggcaaggaa gtgctcttcg gtatccaccc agtcgcagga
      781 aggatgccag gacacatgaa cgtcctcttg accgaggtcg gcatctctta cgagaagctc
      841 tacgatctgg aagacataaa ccccgaattc gaggacaccg acgctgcgat catcgtcggc
      901 gccaatgacg tggtcaaccc ttcggccaac gaccccgaag tggagagtcc cataagcgga
      961 atgcccgtgc tcaccgtcga gaacgcagag cacgtaatct tcatcaagcg ctcactcagc
     1021 ccaggcttcg ccggcgtcga caaccccctc ttctacgaca ccgagaaaac catgatgctc
     1081 ttctccgacg ccaagcaagg cttgcaggac ctcaacgaag ccgtgaagaa cccctagcgc
     1141 acggcgtaca catcgtagcc tgctttcgag gtctccgcgc ccaactcgcg cgtcccaggg
     1201 cactgcaggc ggttgatacg taatccggtt gatcgcttgt ggtggaacgt gcggagcgcc
     1261 tactgcagtg gatttccgcg caaccgggaa tgacgggtga ggctgggcgg ttcgtcggat
     1321 gaaggttgct ccgacttatc tgctcgcggt ggaggcggtg gtttggtgcg agggcaagaa
     1381 gcttgatcag ggcagcggcc ccctgagctg ccactcattc tgcccaaggg ttcgggcccc
     1441 cgaccgagaa gccgcccagc atcccgaccc gaacgacctc tggtttgtgg tctgtcacca
     1501 ggcggagcca cagctttctt cactttgttg ggaaccccgc tgccgagccg gatggtgtcg
     1561 acgggaccca cttgcgtctc gtgaagcctc tgactgtcgg acacgtgggc aggtggatca
     1621 gccggggcag cggcagccgt gcctcccccg ccaggctgtt ccctggccgt aggttctggc
     1681 cgaaccgtgg tagacgccgg cgtggccgtc gcaggcgcgg aggcggatcc ggtgtaagcg
     1741 ggtgacgcgc tcgctgacgc acccgccgat gcgctcgctg acgcgcttgc gaatgtggta
     1801 ccagaggatg atgaagcctg tggcgttcgg gacccatagt cctcttgtcc gctacgctca
     1861 gccctggttc tttcgtcatt gccggtgtct ttagcgggag attcgccctg tttctcaggc
     1921 tggttctcct tgcttacatc ctccgtatta cgttcgtcgt cttccacgtt gccgactagg
     1981 gcgatctcgg agggaagctg ggagaccgtc ggctcttgta ccagtgagac cgaccgtcga
     2041 tcttcccccc tgatgctaga cgcgaggtag gtggcgagga cgaagaccag aaacaccgca
     2101 aggctcacgt acaggcctgg tctgtgaacg ggtgccatcg tgccggcttc gtcctcgacc
     2161 gccccgccga tgccaaccag acggctcagg gatgccagga gtccacgcgg ctcgcgcgac
     2221 aggggatctt ctggtggctc ctgttctgct tgcagggacg cctgacggcg tttgagctcc
     2281 ctggtcatcg ccgctccgtc gagtccaagg tgctcggcgt aggtcttgag ggacccgagc
     2341 atgtacacgg cagggagtac ttcgaagttc tcgttctcca ggtcacggag gtacctggtc
     2401 cggatcttcg tcacctcctc gacctgctgg agcgacagac ctcgcttctc acgggcccgc
     2461 tgcagggtct tgcctatctc tagccccgta tgctggttgc cagcctcgcc gctcatcctc
     2521 acccaggccc gtctaagtct gctccccccg ggtagaccaa catcgcgttc gaataccctg
     2581 gaatgtacca caccccgaag cgtatgggcg cccattcagg gtggcccgtt cctcctcgtc
     2641 agatcgtcga ggcctagagc aatttgcaaa gaggctgaac ctccttgcga acattcgagc
     2701 aacacaaaac tacttcagct tgtgggcgtc tttttctctc ctaaaacggt tttccaagaa
     2761 ggtgagcccc ttcgaaaaca ccctcggggt tgagcctgag ccataagcgg taggccttta
     2821 cttttggctg aagtgctgaa gtgctgagaa gcgaaggcct taaaggcctg agggtgctga
     2881 gggctctttc tcggaaaggt tcatactttt ccagaaaggc tctaggacgt tgcctgcgtc
     2941 agcgggccgt cgtgcgccgg tggagcgaga cagagagcgg tacgaagata aggaggatcc
     3001 ctactcacca ggcgaaggcc agccatgtga gcttacacat tggtcgagga ggaagtctcg
     3061 caccgcgtcg atgacctgtg tcatcggctt gtgcctctcg cccttctaat ctccttagtg
     3121 gccgtcgaag cagattaacc ggtccaggta ctcctcgtcc cgaaaaccca tcttgtcggt
     3181 gccttctcct agactctaca cgatgggtgc aatgagggag tttcaccccg cgtgctcaac
     3241 cagtttggcc tctaggtcga tcgcctccac gcccgcaagc gctttggaaa ccgcgcagtc
     3301 gcgcttggca gcctcggcct tctctaagaa aagttcctca tcgatctcgg ggaccttggc
     3361 ctccgtccgc agctcgatcc ggctgatggc gtacccctcc ccggcgcgcc cgaagcgaac
     3421 ccgagcttcg gtgtggatgc gctccggggg gtacccagcc gccgaaagcc tgcgcgccaa
     3481 cgacatcgaa aagcacccgg cgtgggccgc acccagcaac tcctcggggt tggtgccggg
     3541 gtctgagtcc tccatgcgcg agcggtagga gtaagcgcct tcgaaggcgc cgctgcccag
     3601 ccgcatcttc ccttctccgt cgcgaaaggt gccgtgccag gcagcttgtg ctttacgtat
     3661 gggcattgtc tcgtgctccc ttcccctaga gatgtaacct cagtacatcg ccaactcctc
     3721 gggcccaccc tgctcttacc ctaaccgagc atggagcatt ccggctggag ggaccttctg
     3781 agcatgaccc ctcagcagtt cgggcaacgg gcctcgagat cagcgagtag acgtaccgat
     3841 gtccgggcag ggcaatgcag gatatgcctt gcgagaacat cccagcctct ttacaacgtc
     3901 gatccgggcg cgccgagtat ccgctcaatt ctaccttgta agtatcctag gctcttcacc
     3961 gaagcccaaa gaaggagatt caagtaggtt ttgagtcgcg cttcgtaaga gagatcgcat
     4021 cggagccagg caagccccga gtactatgac cacccgacgg gaccaccagc ggaggagcga
     4081 accgtgtccc aggatgagtg caagcagggc gacaaggtgg agtggaacgc cccgcagggc
     4141 aagacccgcg gcacggtcaa gaagaagctc acctcgagta ccgaggtagg cggacagaag
     4201 atcaacgcct cagaggccaa accgcgctac ctcgtcgaga gcgagaagag cggcaaagag
     4261 gccgcccaca agcccggcgc cctgagcaag gtttaggagg taaggtcgtg gcggaagaca
     4321 ggcagcagat catcgaggag ttcgaagagg ccgtcaacat gacacccaag gagcttgaaa
     4381 ggtgaagatc cacaccgagg actgtgaagt gggcaacgtc ccaggcccag agaccttcac
     4441 aggccacgag ggactgcgcg agttcgggac gagctaccgt agcacctacg gcgagatgag
     4501 gtcggagttc cgagccgttt tctatagccg gcccttcctt cttacggacg aatcacgtta
     4561 gcgaatatct gtggctacct gagcgagatc gtcgagttgc tttaaggttt cgctttcggg
     4621 tagggtcggc aggccgagca agacgcgctc cacgcccagg tgcgcgtatg ctttcaggtc
     4681 ttcgggctcg ctggatgcgt tgaacacgct taccggcacg tctctcccgg caacttctcg
     4741 cagctcgccc atcatgggtt cgagcttatc tgggggaaga ccgtttgcca gccaggcgtc
     4801 gccgaactgg gcgactcgct cgaaagcctg gctaccgccg ccgacgtaga tgggcgggta
     4861 cggcttctgc accggtttgg gccagatgcc gatcgggtcg aagtccacgt actcgccgtg
     4921 gaactccggc tcgtctttgg tccagatctc tatcatcgcc cggatgcgct cgttcatcag
     4981 cttgccgcgg gtgc
//
LOCUS       DASTKM010000158         5344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1097841, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000158 DASTKM010000000
VERSION     DASTKM010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097841"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFQ10_06560"
     CDS             <1..620
                     /locus_tag="VFQ10_06560"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9965039.1"
                     /translation="AATCSPTGYRPTGYPDTQQHTTHFVVADREGNVVSATQTLGNVF
                     GSKVMPQETGIWLNDALAWSRFEPVGNVFDVYPARQPLYALCPTIIMRDGRPRIALGT
                     PGGRTIQQTTPQIITNMLHFEMNVQEAVSAPRFSHVVPDLLVVEEGIPQYVSADLSAL
                     GHDLVPAEYPLGNAHALTIEYGPGGETTRFTGAADPRGGGSATGY"
     gene            complement(634..1011)
                     /locus_tag="VFQ10_06565"
     CDS             complement(634..1011)
                     /locus_tag="VFQ10_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965040.1"
                     /translation="MASNEMPQPPYVLLFEEVVPGARPLTMLVDDRGAMPLFDSEEKA
                     GAFLASTDFGVGLVPVEVSGAGLVRALESVGEDVEYVAINPPPAGEGPMRVRMGGLEE
                     LIEALQTSHQEHDLFGLGSNGSG"
     gene            complement(1015..1335)
                     /locus_tag="VFQ10_06570"
     CDS             complement(1015..1335)
                     /locus_tag="VFQ10_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9965041.1"
                     /translation="METRTVSDALAFSEEKMKKNALFDSPHLFYDAYCLLPGQSQKVH
                     AHEGSDKVYYVLRGTGRFTVAEEERDLGAGNAVIARAGDPHGVRNETQDDLVILVTMA
                     PRPS"
     gene            complement(1420..2589)
                     /locus_tag="VFQ10_06575"
     CDS             complement(1420..2589)
                     /locus_tag="VFQ10_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9965042.1"
                     /translation="MIKDEGQNLLVIGAPEHDADAYHLSGFLAPDAVICLRVAGKRYL
                     AVSSLEYGRAAKSATVDELLSHEELGIISLARELKSGARAYAVAVANLLDELGASNSP
                     VVVPADFGVVYADELRARGITLAPNGKLFDSLRRAKTEEEVSNIEKTQDAVEAACAHC
                     VSILEEAEIGEDEVLNWRGETLTSELLRAEIDVELLRRGCAADGTIVAGGRQAADPHE
                     RGHGPLKAGESIIVDIFPVDLSTRYYSDMTRTFVKGEPNEGLQKMYDAVLESQETALS
                     IIRAGVNGKDVHRKVADVLHEAGYKTNVHDQEEGKPLTEGFFHGTGHGVGLEIHESPS
                     VSLAGDELIPGDVISVEPGVYDPSVGGVRIEDLVVVTEAGCRNLTTFPKEFRIRP"
     gene            2685..2924
                     /locus_tag="VFQ10_06580"
     CDS             2685..2924
                     /locus_tag="VFQ10_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET9965043.1"
                     /translation="MQQRDMHSRVEDALDKVRPALRADGGDARVVACDEKNGSVSIQM
                     LGACKGCPLSQLDFVYAIESLIRREVPEVREIVAV"
     gene            complement(2925..3449)
                     /locus_tag="VFQ10_06585"
     CDS             complement(2925..3449)
                     /locus_tag="VFQ10_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965044.1"
                     /translation="MFKLYLTSILVAEVVAVVALVSLGPGDVRLWYLVPIITLTLVVA
                     FIVRRKFGGPAYGIHRLSPQAIAGYRARDPASNSPEDLSRAIVERAEEIQHALEESPS
                     DEVRVEMCALGYRACANDMITLTHLINEALPDASFLRRVRLRRSRKKAIDALSEARKA
                     LPPGALRTAPQERQ"
     gene            3551..4132
                     /locus_tag="VFQ10_06590"
     CDS             3551..4132
                     /locus_tag="VFQ10_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965045.1"
                     /translation="MQKDPAPEFDLQHPLASAVAVIRTVLFSPRSFYLNFKVEGPLKE
                     PVAFVLLVGAVSGILGAALALSTNLLFGVLDAGDMRAAVVDAVAFALLSPIGVGVVAG
                     VYLLSIRTFVGKTSGFREVYRMAAYAAAALALAWIPVLGAFAFAYALIVLMGIGIQSV
                     YGTTFLTTVVTAVVGFVPVASVLIWLIISGVAS"
     gene            4215..4823
                     /locus_tag="VFQ10_06595"
     CDS             4215..4823
                     /locus_tag="VFQ10_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965046.1"
                     /translation="MRGGCKHSILPTVTIRPQELAHALTNALNPFVIFTALFALAAFA
                     EAGAYRGVLYLAVELTAGAAVAGYVLFMRRRSRVGDFWISVRAERLTPAVFLLAAFVA
                     LLAALVLLEAPQNLTLLTFSMGLASAGVAGITLLWKASAHCTVAGHAAAAGPLLLGPL
                     GFVFLLVLPLVLWSRVTLKAHTLSQTLAGAAVGAGCAVPFLA"
     gene            complement(4854..4921)
                     /locus_tag="VFQ10_06600"
     tRNA            complement(4854..4921)
                     /locus_tag="VFQ10_06600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4869..4871),aa:Ser,seq:cga)
     gene            5012..>5344
                     /locus_tag="VFQ10_06605"
     CDS             5012..>5344
                     /locus_tag="VFQ10_06605"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965047.1"
                     /translation="MARKNGNGEGSRPRERSDGRWEARYWAGDKRRSVYGSTRKEAAD
                     KLARAIANNEESYPTLAATDITVREFFAQYDDAVRDTMKRRSLETYRDIARLHLLPAF
                     GGKKLRDLT"
BASE COUNT          922 a         1713 c         1694 g         1015 t
ORIGIN      
        1 ccgccgcgac ctgctctccc accggctatc ggccgaccgg gtatcccgac actcagcaac
       61 acaccacgca cttcgtcgtc gccgacaggg agggcaacgt ggtctctgcg acgcagaccc
      121 tgggcaacgt cttcgggagc aaagtcatgc cgcaggagac cgggatctgg cttaacgacg
      181 ccctcgcgtg gtcgcgcttc gagcccgtgg gaaacgtctt cgacgtatat cctgcccgcc
      241 agcccctcta cgcgctgtgc ccgaccataa tcatgcgcga cgggaggcct cgcatcgcgc
      301 tcggcacccc tggcggacgc accatccagc agacgacgcc gcagattatc accaacatgc
      361 tccacttcga aatgaacgta caagaggctg tctccgcgcc gcgcttcagc cacgtcgtcc
      421 ccgacctgct cgtggtcgag gaaggcatcc cacagtacgt cagcgccgac ctctccgccc
      481 tgggccacga cttggtccct gccgagtatc ccctcggcaa cgcccacgcc ctgacaatcg
      541 agtacggtcc cggtggagag acgacccgct tcaccggcgc cgccgacccg cgcggcgggg
      601 gctccgctac aggatactga gcgcgtcctg gtgtcaaccg gagccgttgg agcctagccc
      661 gaagaggtcg tgttcttgat ggctcgtctg gagggcttcg atgagctcct cgaggccgcc
      721 catccttacc ctcatgggac cttcaccggc aggcgggggg ttgattgcga cgtactcgac
      781 atcttccccg acggactcga gcgccctgac gagaccggcg cccgagacct cgacaggcac
      841 tagcccgaca ccgaagtccg tggaagccag gaacgctccg gccttctcct cggagtcgaa
      901 gagcggcatg gcgccgcggt cgtccaccag catggtgagc ggcctggcgc ctgggaccac
      961 ctcctcgaag aggagcacgt agggaggctg cggcatctcg ttcgaggcca cggctcagga
     1021 ggggcggggg gccatcgtca cgaggatgac caggtcatcc tgcgtctcgt ttcggacgcc
     1081 gtgggggtcc ccggcgcggg cgataacggc gttgcctgcg ccgagatctc gctcctcctc
     1141 agccacagtg aagcgacccg tgcctcgcag gacgtagtag accttgtccg agccctcgtg
     1201 ggcgtggacc ttctgggatt ggccggggag gaggcagtag gcgtcgtaga agaggtgcgg
     1261 cgagtcgaag agggcgttct tcttcatctt ctcctcggag aacgctaggg catccgaaac
     1321 ggttctggtc tccacgacat ttcctccttg gatcttattg atgtccactc cagcgtcccc
     1381 gcggctcgac cggtgaggcg gacatcaagc tgtgcgtttc tacggtctga tccgaaactc
     1441 cttggggaac gtcgtcaggt tgcggcagcc tgcttcggtt acgactacca agtcttcaat
     1501 gcggacgccg cccacgctcg ggtcgtagac gccaggctcg actgagatga cgtcgccggg
     1561 tatgagttcg tcaccggcca gagagacact gggggactcg tggatctcca gtccgactcc
     1621 atgccccgtg ccgtggaaga agccctcagt gagcggctta ccctcctctt ggtcgtggac
     1681 gttggtcttg tatcccgctt cgtgcaggac atcggcgacc ttacggtgga cgtccttgcc
     1741 gttgacgcct gcccttatta tggagagcgc cgtctcctgg ctctccagga cggcgtcgta
     1801 catcttctgc agcccctcgt tcggctcgcc cttgacgaag gtgcgggtca tgtcggagta
     1861 gtagcgggtc gagaggtcaa cggggaagat gtcgacgatg atggattctc ccgccttcag
     1921 gggcccgtgg ccgcgctcgt gcgggtctgc cgcctgccgg ccaccggcca cgatcgtgcc
     1981 gtcggcggcg cagccgcgcc tgaggagctc gacgtctatc tcggcgcgca gcagctcgct
     2041 cgtaagggtc tcgccgcgcc agttgaggac ttcgtcctcc ccgatctcag cctcctccag
     2101 gatactcacg cagtgagcgc aggcggcctc gacggcgtcc tgggtcttct ctatgttgga
     2161 gacctcctct tccgtcttgg cccggcgcag gctgtcgaag agtttaccgt tgggcgccag
     2221 ggtgatgccg cgggcgcgca gctcgtcggc gtagacgacg ccgaagtcag caggtacgac
     2281 caccggagag ttcgaggcgc cgagctcgtc gaggaggttg gccacagcga cggcgtaagc
     2341 cctggcgccg ctcttcagct cgcgggcgag gctgatgatc cctaactcct catgagagag
     2401 tagctcgtcc acggtcgcgc tcttcgcggc ccgcccgtac tccagcgaag agacggccag
     2461 gtacctcttg ccggccacgc ggaggcagat cacagcgtcg ggggcgagaa accccgagag
     2521 atggtaagcg tcggcgtcgt gctccggcgc cccgataact agcaggttct gtccttcgtc
     2581 cttgatcaca aacatccttc cctaaacggg tcatctcagt cgccccattc tactatcgta
     2641 caggacgaaa tccggtccca taaccggata taatcggcgg cgagatgcag cagcgggaca
     2701 tgcacagcag ggtagaggat gcgctggaca aggtacgccc ggcgctgcgt gccgacgggg
     2761 gagacgccag ggtcgtagcc tgcgacgaga agaacggttc cgtcagcatc cagatgctag
     2821 gggcctgcaa gggatgcccg ctctcgcaac tcgacttcgt ctacgctatc gaaagcctca
     2881 tcagacgcga ggtgcccgaa gtgcgggaga tcgtagcggt atagctattg cctctcttgc
     2941 ggtgctgtac gcagggcgcc cggcggtaga gccttcctgg cttcggagag ggcgtcgatc
     3001 gccttcttgc ggcttcggcg gagcctcacg cgtctaagga aggaggcatc ggggagggct
     3061 tcgttgatga ggtgagtcag cgtgatcatg tcgttcgcgc aagccctgta gcccagggcg
     3121 cacatctcga cacgaacctc gtctgaggga gactcttcga gggcgtgctg aatctcctcg
     3181 gcccgctcta ctatggcgcg ggagagatct tcgggggagt tgctggcggg atcacgggcc
     3241 ctgtagccag cgatcgcctg gggcgagaga cggtgtatgc cgtaggcagg accgccgaac
     3301 ttgcggcgga cgataaacgc taccacgagc gtcagcgtaa ttatggggac gaggtaccag
     3361 agacgcacgt cccccggccc cagggacacg agcgccacga cggcgactac ctcagccacc
     3421 agtatcgagg ttaggtagag cttgaacatc gcctacgata cctctccgcc accattttcg
     3481 agggacctaa gtctaagttt ctgagagcga tttcgcaagc acggtcagga cggtctgtat
     3541 aatttcgcct atgcaaaaag atccggcccc cgagttcgac ctgcaacacc ccttggcgag
     3601 cgcggtcgca gtgatacgga ctgtcctctt ctctccgaga agtttctacc tgaacttcaa
     3661 ggtcgagggg cctctgaagg aacccgtggc cttcgtgcta ctcgtcggcg cggtctccgg
     3721 catcctcggc gccgcgctcg ccctctccac caaccttctc ttcggcgtcc tcgacgccgg
     3781 tgacatgcgg gcggcggtgg tcgacgctgt tgccttcgca ttgctctcgc cgatcggggt
     3841 tggcgtggta gctggagtgt accttctctc cataaggacc ttcgtcggga agacgtccgg
     3901 cttcagggag gtctaccgca tggcggccta cgccgcggcc gccctggcac ttgcatggat
     3961 acccgtactt ggcgccttcg cgttcgccta cgccctgata gtgctgatgg gaataggcat
     4021 ccagagcgtc tacgggacca ctttcttgac cactgtggtc acagccgtgg tgggcttcgt
     4081 acccgtcgcg tcggttctga tctggctcat catctcaggg gtggcctcct agagagcgca
     4141 tcgtactaga gtaggtctcg ctccaccctg tgccccatgg ccacggacca aagcgcgagg
     4201 gtcggactgt cgatatgcgc ggaggttgca agcactctat cctacccacc gtgaccatcc
     4261 gacctcagga actcgcccac gcccttacga acgcactcaa cccgttcgtc attttcacgg
     4321 cgctctttgc cctggccgcc tttgcggagg ctggtgccta caggggcgtc ctctacctgg
     4381 cggtggagtt gaccgctggg gcggccgtcg caggatacgt gctctttatg cgacggcgct
     4441 cgcgggtggg ggacttctgg atctccgtcc gcgccgaacg tctcacgcct gccgtgtttc
     4501 tgctcgcagc cttcgtggcc ctgctcgcgg ccctggtact gctcgaggcc ccacaaaacc
     4561 tcactttgct gaccttttcg atgggtctcg cgagcgcggg ggtcgccggg atcacactgc
     4621 tctggaaagc aagcgcccac tgcacggtcg ccggacacgc agccgctgcc gggcccctac
     4681 tgctcggccc tttaggcttc gtcttcctcc tcgtgttgcc gctcgtactc tggtcgcggg
     4741 tgaccctcaa ggcgcacacc ctgtcccaga cgcttgctgg cgcggcggtg ggagcagggt
     4801 gtgcggttcc ctttctggca tagaatcagc ccgccgaaga gcgggtttgg tagtgacccc
     4861 accgggattc gaacccgggt tttcgccgtg agagggcgat gtccttggcc tctagggggc
     4921 cttgaaacag cgggcttcgc gctttcggga gggttttgac cccaacggtc gacccctacc
     4981 gcaccgaacc atagaccaca ggaggtggtt tgtggcgagg aagaacggca acggagaggg
     5041 tagccgcccg cgcgagaggt cggacggacg gtgggaagcg aggtactggg caggcgacaa
     5101 gaggcggagt gtttacggaa gcacccgcaa ggaggccgct gataagttag cgagggccat
     5161 agcgaacaac gaggaatcct atcctacgct cgccgcgaca gacataacgg ttagggagtt
     5221 cttcgcgcaa tacgacgatg cggtgagaga taccatgaag aggcgcagct tggaaaccta
     5281 ccgcgacatc gcgcggttgc atctgctccc agcattcggt ggcaagaagc tcagagacct
     5341 gacc
//
LOCUS       DASTKM010000159        20024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_569399, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000159 DASTKM010000000
VERSION     DASTKM010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 20024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20024
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569399"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFQ10_06610"
     CDS             complement(<1..355)
                     /locus_tag="VFQ10_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965048.1"
                     /translation="MDPQRESLRQLLKDYYTKYYAELRRQLPKYAAASEQVPPFLRSH
                     ARIIFISECADGYVITHRTNPTTAGDAAETFVMIADDWDRTVGDATGKDYDPYSDFDE
                     SEAPFAREVTSGEYAE"
     gene            complement(725..1450)
                     /locus_tag="VFQ10_06615"
     CDS             complement(725..1450)
                     /locus_tag="VFQ10_06615"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9965049.1"
                     /translation="MARIAWALSDPVRLSVMQILMGGPAAVAELVSATGASQPNVSNH
                     LRVLREQSLVRSERRGRQAIYEIPNASVAQLVESLTAVAGGPTAPVFETGAMSEARTC
                     YDHLAGRLGVGIYGALVDAGAMTPTSEVRGDVLLGPAAEEVFGELGIDPGAVAEKSGR
                     RRFAFACPDWTERLPHLGGALGAAVCERFFEEGKVRRLEGTRAVALTGKGERTLQEHL
                     GLRISRVIGPDRPAGDTRQGELS"
     gene            1582..2475
                     /locus_tag="VFQ10_06620"
     CDS             1582..2475
                     /locus_tag="VFQ10_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET9965050.1"
                     /translation="MLKAKADLLRGLHHGDELLVLPNAWDCMSARVLEDCGFPTIATT
                     SAGIAWAFGYPDGEGLGRDEMAEATARIAGAVTVPVTADAEAGYGATPDAASETALAV
                     IRAGAVGLNLEDPVDTEPPGGVGSFGNGDASELVELEEQAERIRAAVEAGREAGVPLV
                     VNARTDVYWREVGDPDERFAETVRRGRAYVEAGAECVFVPGVKDPDTIAALVREIPGP
                     LNMLAGPGSPTVAELARLGVRRVSVGSGPARAAMGLTRRIGQELLRSGTYSSLGGDAL
                     PYAEANALFEGHSRSEPPGGI"
     gene            complement(2509..3069)
                     /locus_tag="VFQ10_06625"
     CDS             complement(2509..3069)
                     /locus_tag="VFQ10_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965051.1"
                     /translation="MDPRVTSVPSSARVSIPDAARLVDAVLERSTGKHSSIHGESHWQ
                     RVAAAALALLPETPDADPALVFLFALFHDSMRLNDGHDPLHGPRGAALARELRGGPFE
                     LEDAEMDLLAFACEEHTNGGVNPDPTVGVCWDADRLNLWRVGIQPDPRFLSTQAARSE
                     VRIAWARDLQQEHLAWKDLNRAFGLR"
     gene            3358..3948
                     /locus_tag="VFQ10_06630"
     CDS             3358..3948
                     /locus_tag="VFQ10_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965052.1"
                     /translation="MELTDKELRDRGIRRSPEDIAEEARRFVFYVISEMPTGRWVADP
                     GREFTEEEIAVLNEGGLDLAPWEAGPDPLARTAAKYVALLASALTTKEAAGVLGVGES
                     RVRQRLGEGTLYGVKAGRENRLPAFQFEGGNEVPGIAEVLTRMDRSLHPVAVLNWFMV
                     PNPDLYLDEEARPVSPRYWLLSGGDPEVLAHLAQEL"
     gene            3956..4591
                     /locus_tag="VFQ10_06635"
     CDS             3956..4591
                     /locus_tag="VFQ10_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HET9965053.1"
                     /translation="MAKLPEPPSVAMLRRVPPAVATLQAGTKLWRIYFRDGKYPSTWD
                     QMRHYGPVGNARFDHHLDPQRAQDRGILYAATGDDAIATCLAEVFQDTRLVDTRRNEP
                     WLAAFTLLEDVSLLDLSGKWPTVAGASANINSGPRPRCRRWSQTIYDSYPDLMGLYYG
                     SCMNGNEPAVVLYERASKALPRLPLFNRPLSDAPLLISIERITAELGYDLI"
     gene            4821..5354
                     /locus_tag="VFQ10_06640"
     CDS             4821..5354
                     /locus_tag="VFQ10_06640"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965054.1"
                     /translation="MFWTDVEIKATATLEVLDDFLRQEWLECCGHLSAFYIDEDTYMV
                     PSAYRMEQFGLFADPNERSMKAKLGGILSKGSRFQHVYDFGTSTDLKLRVADERQGRV
                     GSEPVRLLSLNEAPVWEWEVCGEEASWICTFCIYERQNPFYCELHSEDHDCEESEMLL
                     PVVNSPRMGMCGYTGPD"
     gene            complement(5617..6513)
                     /locus_tag="VFQ10_06645"
     CDS             complement(5617..6513)
                     /locus_tag="VFQ10_06645"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HET9965055.1"
                     /translation="MDETLAQALQSLEIRGPVSHGLLHMFPLQGDTQAEQDISLLEGA
                     LQAGTLHIKEPHEAGSVPELRVVNEGSLRVLILEGDKLIGAKQNRVVNSSVLVAAGSD
                     LTLPVSCIERGRWSYRSRAFSPGTGSPHLALRRLETRSVHDSLRRGRGHRSDQGAVWK
                     EVRRKARLHAAASRSPLSERLDAFEKLARDLPEGTSGVVVAIGERLVLLEVLAGPRTF
                     ARVFRKLLSGYALESVGLDRPYGTPDPPAVRSFIEAAAKAAHEEHQAVGVGRDVRFEE
                     GGISGYALIGEEGVLHAAAFGG"
     gene            complement(6683..7222)
                     /locus_tag="VFQ10_06650"
     CDS             complement(6683..7222)
                     /locus_tag="VFQ10_06650"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET9965056.1"
                     /translation="MDDPRYIVGAGADNGVLLGAFLELLLIIANIGTAVVLYPVVKRQ
                     NEILALGYVTARIVECAFIAVGLLSLLSVVTLRQEAAGADAGSLVAVGESLMVLHDWT
                     FVLGPGFVVGLGNGLILGYLMYRSALVPRAMAMLGLIGGPLIIASGVAVLLGVIEAGS
                     VWQAIATIPEFFWELSLGI"
     gene            7290..7736
                     /locus_tag="VFQ10_06655"
     CDS             7290..7736
                     /locus_tag="VFQ10_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965057.1"
                     /translation="MDQPTAEEALKRLEPLVGEWILEAKPPDGPPLPGEARATIEWHD
                     SGAHLVQRSTVEMPEAPDSISIMGCDAANGTYFQLYSDERGVCRVYEMSISDGRWKLW
                     REGEPFPKRFIATISDDGKTIAGRWEKAPDGRTWETDFDFVYRKVR"
     gene            complement(7919..8185)
                     /locus_tag="VFQ10_06660"
     CDS             complement(7919..8185)
                     /locus_tag="VFQ10_06660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9965058.1"
                     /translation="MWGSILLGLGTAMVYPSLIAAVSDNSHPSWRARSLSVYRFWRDL
                     GYAVGALCAGIIADTLGLSWAIGSVGVITFLSGVVVAMFLGEKE"
     gene            complement(8131..9102)
                     /locus_tag="VFQ10_06665"
     CDS             complement(8131..9102)
                     /locus_tag="VFQ10_06665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9965059.1"
                     /translation="MSQFSLLVVVNAFVGGMVGLERTVVPLIGSQQFGLVLKSAIFSF
                     IISFGVVKACSNLVSGTLADRVGRKKVLVAGWVVGVPVPFMIALAPSWGWIVAANVLL
                     GVNQGLAWSMTVLMKIDLVGPRGRGLAVGLNEFAGYLAVGLTALATGYLASVYGLRPE
                     PFYLGIGYVTLGLLISVFLVRDTTEHVRVEMEQHPPESAPLTFREVFSRTSFKDRHLF
                     ACSQAGLVNNLNDGMSWGVFPLFFSAFGLGVEAIGVLKAVYPAVWGCLQVLTGPLSDR
                     WGRKGLIVAGMWVQAGGSSSPWRTGASGGGCGGASSSASARRWCTHP"
     gene            complement(9143..9874)
                     /locus_tag="VFQ10_06670"
     CDS             complement(9143..9874)
                     /locus_tag="VFQ10_06670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9965060.1"
                     /translation="MGELAMWQQRVEEFVLDQVGLPPARVLEVGCGEGELAHALARAG
                     HSVTAIDPQAPEGPIFRRVGIEEFTDPDPFDHVVASLSLHHIENLGGALDNIADMLRT
                     GGSLVVIEYAWDRIDEATAEWALERLPVASLSEKPSWLGRRCQEWVRGGPGGERARAE
                     SYFAEWASEEGFHSSRRMRDELGSRFVERFFEWVPYLYPELGEDTEEADESAAIEART
                     INATGFRYVGATMPETERSLAPRRG"
     gene            complement(10069..10962)
                     /locus_tag="VFQ10_06675"
     CDS             complement(10069..10962)
                     /locus_tag="VFQ10_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9965061.1"
                     /translation="MNLEESAVGRLLRMEEVIPAMERALADFSNGTVVQPVRTMLPVA
                     EHGGFLGLMPAYTGSALGAKLVAFYPRNTSVPTHYATILLFEPETGEPLVTMDGRLIT
                     EVRTAAVSAVATDHLARADASVLAIIGSGVQARSHLEALRLVREFREVRVWSPRRAAA
                     FAEEHGVDAAASAEEAVRGADVVVTATTSPTPVLYGEWLSPGAHINAVGAPRPDWREL
                     DDEVLRRAKVYVDSREAAMKESGDVIAAREVLAEIGEVVSGAEQGRRSLEEVTLFKSL
                     GLAVEDVATAELVYRNALGGA"
     gene            complement(11288..11770)
                     /locus_tag="VFQ10_06680"
     CDS             complement(11288..11770)
                     /locus_tag="VFQ10_06680"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET9965062.1"
                     /translation="MLWDQAFLWIGRRRMTREEDSPRIRGTGGCLCGAVRYEVRGPLC
                     PVVDCHCTMCRRTSGHFAAFTATRPEDLVLIESGGLRWYRASAKARRGFCGYCGSSLF
                     WEPASGERVSIAAGTLDLPTGLETAVHVFVEDSGDYYMISDGLPQRVDGNHGIMIPAD
                     "
     gene            complement(12414..12959)
                     /locus_tag="VFQ10_06685"
     CDS             complement(12414..12959)
                     /locus_tag="VFQ10_06685"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HET9965063.1"
                     /translation="MMLMPNTSNPGVLPEPTLQDAIPTLSRLVRDPAFLDSHVLPHLE
                     EAGRAEDWYVAYRREDPDRYCSLQIFVWPPGSATAIHDHSSWGAFCCVVGSVLEERYE
                     RADDGSMPDHARLKELWRLEWRRDDGISTVLPYEGGIHRVGNPTGEPAISVHLYGPRL
                     GEIDGRDYDPSRDYVCDRLED"
     gene            complement(13099..14832)
                     /locus_tag="VFQ10_06690"
     CDS             complement(13099..14832)
                     /locus_tag="VFQ10_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9965064.1"
                     /translation="MRHRVEVPSRSRLLLGVRSGPRHCTGAVGVAVAEAIEDLGRGRE
                     YYARRAWMAAYESLSRADRTAPLGAEDLELLATSAFMLGRDDECLSGLERAYHVYLDG
                     GEAMRAVRCAFWVGMNLALRGEMGRATGWLGRAQRLVEREGSDRVERGYLLVPVMLRH
                     EASGDWEAAYATAADAAEIGERFGDQDLLALAVHEQGHALVKQGRVEEGLGLFDEAMV
                     AVTAGELSPIVTGMVYCSVIMGCQEVYALHRAQEWTAALTQWCEQQPEMVAFSGRCLV
                     HRAEIMELHGAWREALEEARRAHQRSVQGKNQPAAAQAFYRQGEVHRLRGEFSAAEEA
                     YRDASRCGCEPQPGLALLRLAQGNGDAASAAIRRVVGETTEPLKRARLLPAYVEIMLA
                     MGEAEEARSACRELEEVLAQYKSGMLSAMVAHAEGAVALTQGDARAALPALRHAWQVW
                     QELEVPYEVARVRVLLGQACRALGDDDTAALELEAARGVFEQLGATPDLTKVDSLTRD
                     APSVDAHGLTPRELQVLRLVAAGETNKAIAAELVLSDRTVDRHVSNIFTKLGVSSRAA
                     ATAYAYEHQLV"
     gene            15315..16067
                     /locus_tag="VFQ10_06695"
     CDS             15315..16067
                     /locus_tag="VFQ10_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9965065.1"
                     /translation="MLFGEAKEEVGSGSAGDAMVEANDLEKTYAAGGATVHALRGVDL
                     SIPRSEMVAIMGPSGCGKTTLLNCLSGLDEFDSGEVFVGGESISGMSDRRRTRFRAEQ
                     MGFVFQTYNLIPVLSGIENVELPLLVAGVRSKEARGRALSALEMVGLADQANKRPNEM
                     SGGQQQRVTVARSLVNEPSIVWADEPTGALDSETSAEIMDLLAKLNQEKGQTFVLVTH
                     DISVARRASRLVTMRDGRIQSDEPAVKGERDV"
     gene            16060..19260
                     /locus_tag="VFQ10_06700"
     CDS             16060..19260
                     /locus_tag="VFQ10_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9965066.1"
                     /translation="MFEFLGVPAERLTLILSGVFAAGALTLAFLAARDRTSFRMAVRN
                     IPRRPTQTALILLGLMLATLLFSAAFTTGNTLTNSLRTQALENIGRVDVVVKAEQPES
                     GSVAFGPGAGGAPDLETRERYFDESLVGKIRGHLSEVESVAGVAPLAKESVPVTSPKT
                     DLSEPQVDVLGMDANSMRGFDRLRTASGTPLGLGDLEKNEVYVGTETAEGLGIGVGDR
                     VEVSLIQPPSKPQSKQRLGTAQGRPRGPEARMPPAGAQSGERPPGSTEDARPAGSEVE
                     TRPAGAKSKERPPEFAVAGVYESGANPASDTSMVMPLTQMQRLTGEGGKVNEILITHG
                     GPATEGGEHTSKTVDKIRPVVSANSLEADPVKKEAIEAADQRGETFSALFVLFGQFSV
                     AAGMLLIFLIFVMLAAERKRELGMARAVGMQRSNLVRTFAFEGALYALAASAIGSVAG
                     VGVGWVMVRLVGRVLAGGEGDFRIAFASSPENVVLAFCMGMVLTFAVVLISSWRVSRL
                     NVVRAVRDLPEPDRRGRSVWGVLVAVTLPLAGSVAFWQGLATQTMAFYLGGISLLLIG
                     AALLARVLGLPDRVAFTASGLLLLALWLVPARITAPAGMAKGPEMFFVSGIAIVVAGV
                     WLVIFNTDVLLWIVVAIFGRAKGLPPVLKTAVKYPTQSLFRTGMTLAMFMLVVFTLTA
                     MNFIQAAMSAAFEDTQALSGGYEVQADASYASPIPDLAAAIEEADGIKSGDVRAVGEV
                     SNLPLEVKQRGTNRDPRPLYVQGVDGGYSDSVDYGFETTARGYGSAREVWKALQTEKD
                     TAVISSGLAPARNVSTFGSSAEPPVRLTGFYADDKNLPEDLYLRIEDPESGQTRNLRV
                     IGVLGSSASFAGDVVTSQGALEGLAGRPVASQRYYFDLSSGVDAAITAKTLEKDFAKN
                     GLQTQVTAEVIRDNDATRRIVFFLLRGFMSLGLVVGIAALGVIAARSVVERRQQIGMM
                     RALGFQRRQVQLVFLTESSFIALLGIGVGVALGLGFSGTLIDNISQNIPGLEYTVPWG
                     ALLLVMIFGYAASLLTTYLPARQASKVYPAEALRYE"
     gene            19438..>20024
                     /locus_tag="VFQ10_06705"
     CDS             19438..>20024
                     /locus_tag="VFQ10_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965067.1"
                     /translation="MTQTRPGSEVTAPAGSIDHEVMSVSQNPTAAAQERNEGPPTGSA
                     NTSKMGSDTSLRNLLPLAWPLFALMWLVFPLASVVDMLGKDLAPIRLLAFLALTVAYV
                     AVYLWLVLRYPFRSGEMSPSERRFHVALLLVLAALALCLEVAFGSGIPYQFMFVVIAA
                     AVTLPTIPAVSTVIALIVLTSVIYALRSGWDTLASS"
BASE COUNT         3752 a         6534 c         6128 g         3610 t
ORIGIN      
        1 gctcggcata ctccccactc gttacctctc tcgcgaaggg agcttcgctc tcgtcgaagt
       61 ccgagtaggg atcatagtct ttccccgtgg cgtctcctac agtccggtcc caatcgtcgg
      121 caatcatcac aaaagtctcc gcagcatcgc ccgcagtcgt tgggttggtc cggtgagtta
      181 tcacgtagcc gtctgcgcac tccgagatga agataatccg tgcgtggctt ctaaggaacg
      241 gtgggacttg ctctgaagcc gcggcgtact tcggcagttg tcttctgagc tcggcgtagt
      301 acttagtgta gtaatccttg aggagctgcc ttagactctc ccgctgcgga tccatgagtg
      361 ccctcttgat tattggacga caccacgctt catctaagtc cctgccaatg tctactcaga
      421 ccggtttagc gatgtagtct cagctctctc ctcgcggctc tcgatccacg ccgttaggag
      481 catattactc cttgtggaat cacttagagg tcaacggaca acttccgtgg gtgcaatgac
      541 cacagcagtt gagacaagat gtagagtttg caacgagatg atgcactact gattgtgcaa
      601 aagaaagatt ctggaaggta catcggtcag acaaacgccg aggcccagag gaccaccgta
      661 acgagcgccg ccacccatgc ctgcagggtt tcccttccgc cgacgctctc cgatgtccct
      721 tcgctcaaga cagctctcct tgcctagtat cgcctgccgg gcggtcggga ccgatcacac
      781 gggagatccg caaccccaga tgctcctgta aggtgcgttc tcccttaccc gtgagggcca
      841 ccgcccgcgt gccctctagc cggcgtacct taccctcctc gaagaaccgt tcgcacaccg
      901 ccgcccccag agcgccgcca aggtggggca accgctcggt ccagtcaggg cacgcgaagg
      961 cgaagcgacg acgcccggat ttctcagcca ccgcgccagg atctatcccc agctcaccga
     1021 acacctcctc ggcggccggg ccgagcagca catcgccgcg gacttcgctg gtcggcgtca
     1081 tggcgcccgc gtccaccagc gccccgtaga tcccgacccc caacctcccc gccaggtgat
     1141 cgtagcaggt cctcgcctcg gacatcgcgc cagtctcgaa gaccggcgcc gtgggaccgc
     1201 ccgccaccgc ggtcagagac tccacgagct gcgccaccga ggcgttgggt atctcgtaaa
     1261 tggcctgtct tccgcgccgc tcgctcctca ccaagctctg ctccctcaaa accctcagat
     1321 ggttggagac gttcggctgg gaggcgcccg tcgccgatac cagctccgcc accgccgcag
     1381 gcccgcccat caggatctgc atcacggaca gccgcaccgg gtcggagagc gcccaagcga
     1441 tccgcgccac attttcgaga tcctgcacta ccctctcaca tctcgtaata atatatcagt
     1501 gaactatgat atattaacgc aaagtcgcag cggtgcaaga tcgggagcgc agtaggctgg
     1561 gaaaggagcg gtattttgga tgtgctcaag gcgaaggcgg acctgttgcg ggggttgcac
     1621 cacggggatg agttactggt gctgcccaat gcctgggatt gcatgagcgc gagggtcctc
     1681 gaggattgcg gatttcctac gatcgccacc acgagcgccg gcatcgcctg ggcgttcggt
     1741 taccctgacg gcgagggact cggccgcgac gagatggcgg aggcgacggc gcgtatcgct
     1801 ggcgcggtca cggttccggt cacggccgac gctgaggccg gctacggggc cacaccggat
     1861 gcggcctcgg agacggctct ggcggtgatc cgggccggcg ccgtgggcct gaacctcgag
     1921 gacccggtgg ataccgaacc accgggcggc gtgggctcct tcggtaatgg cgacgcctct
     1981 gagcttgttg agctggagga acaggcggag aggattcggg cggccgtcga ggctggccgg
     2041 gaggccgggg tgccgctggt ggtcaacgcc aggaccgacg tttactggag ggaggtcggg
     2101 gatcccgatg aacgctttgc ggagacggtt cggaggggta gggcgtatgt ggaggccggc
     2161 gccgaatgtg tcttcgtgcc tggcgtcaag gacccggaca ccatagcagc cctcgtcagg
     2221 gagataccgg gccccctgaa catgcttgca ggccccggaa gtccgaccgt cgccgagttg
     2281 gcgaggctgg gcgtgcggcg cgtcagcgtg ggctcgggac ccgcgagggc ggccatgggg
     2341 ctgacgcgca ggatcggtca ggagttgctg cggagtggca cgtactcctc cctgggcggt
     2401 gacgcgcttc cctacgccga agccaacgcc ctcttcgaag gtcactcccg gtccgaacct
     2461 ccgggaggga tctagcggcg tcccccgact catctgtggt ccggcgaatc atctgagtcc
     2521 gaatgccctg ttgaggtcct tccaggcgag atgctcctgc tgcaggtcgc gcgcccacgc
     2581 gatgcgcact tcgctcctcg ccgcttgcgt cgagaggaac cgagggtccg gctggatccc
     2641 cacccgccac aggttcaacc tatcggcgtc ccagcacact cccacggtcg ggtctgggtt
     2701 gacgccgccg ttggtgtgtt cttcgcaagc gaacgctagc aagtccatct ccgcgtcctc
     2761 gagctcgaac ggcccgccgc gcagctcccg agccagagcc gcccctctcg ggccgtgcag
     2821 ggggtcgtgg ccatcgttga gcctcatgga gtcgtgaaag agggcgaaca ggaacacgag
     2881 tgccgggtcg gcgtcgggcg tctcgggaag gagggcgagg gccgcagcgg cgacccgctg
     2941 ccagtggctc tcgccgtgta tggaggaatg cttgcccgtg gacctctcga gtacggcgtc
     3001 cacgagtctt gcggcgtccg gtatggagac acgggcactt gaaggcacgc tagttacccg
     3061 agggtccatc gttgggcttt cgcaggcggt acctcacctg caactctgcg gtcacgctag
     3121 atcactcctc ccgttcgttt ctctgccagc gacgatgctc agtcgagacg tacctgctcc
     3181 ttcgattgcc gaaccctccg agaacccgtt gtaagggtgc aacaccaaac aacgagcccg
     3241 agcaagactc tgcatgccag aaagcattgc acatatcttc tgcatagcgt agcattacta
     3301 acgtgggcta acattgtaat tttgcgttaa cagaacgatt atggagaggt tggggctatg
     3361 gagcttacgg acaaggaact cagggatcga gggataagga ggagtcctga ggacatcgct
     3421 gaggaggcgc gacgctttgt gttctacgtc atctccgaga tgccgacggg ccggtgggtg
     3481 gccgatcccg gcagggagtt caccgaagag gagattgcgg ttttgaatga gggaggtctg
     3541 gacctcgcgc cgtgggaagc ggggccagat cccctggcca ggacggcggc caagtacgta
     3601 gccttgctcg cttcggccct caccacgaag gaggccgccg gggtgctcgg ggtgggcgaa
     3661 agccgtgtcc gccagaggct aggtgaggga acgctctacg gagtgaaggc gggaagggag
     3721 aaccggttac ccgctttcca gttcgagggc ggcaatgagg tgcccggaat agctgaggtg
     3781 ttgacgcgta tggaccgctc cctgcatccg gtggcggtcc taaactggtt catggtgccc
     3841 aaccccgatc tgtacctcga cgaagaagcg agacccgtct ccccgaggta ctggctgctc
     3901 tccggcggcg acccggaggt cctggcgcac ctggcgcaag aactttagga tcagggtggc
     3961 caagctgccg gagcctccgt ccgtcgcaat gctgagacgc gtcccgccgg ccgtggcgac
     4021 gctgcaggcg ggcacgaagc tctggcggat ttacttcagg gacgggaagt acccgtctac
     4081 gtgggaccag atgagacact atggacccgt aggtaacgca cgcttcgacc accacctcga
     4141 tcctcagagg gcgcaggatc gcggcatcct ctatgcggct acaggcgacg acgccatcgc
     4201 cacttgcctc gccgaggtct tccaggacac gcgcctcgtg gacaccagga ggaacgagcc
     4261 ctggctcgcc gcgtttacct tgctggagga cgtatcacta ctcgacctca gcggcaagtg
     4321 gccgaccgtg gcgggggcat cggcgaacat caactcgggg ccgaggccca gatgccgacg
     4381 ctggtcgcag acgatctatg attcctaccc cgacctcatg ggcctgtact acggctcgtg
     4441 catgaacggt aacgagccgg cggtggtcct ctacgagagg gcctcgaaag cgctcccgcg
     4501 cttgccactc tttaataggc cgctctccga cgcaccgttg ctcatctcca tcgagaggat
     4561 caccgccgag ctcggctacg atctgatctg aaagcggttt ccttcgcaaa tcatggcatc
     4621 aaagacttgc gcagttggag acgatagaat aaccggcatg agtcggttca cctcatacgg
     4681 tatctgcacc ctctgtggca agcgcactac ataagcgggg atgacgcgcc cacctcagca
     4741 gctgtctggc tgagcacgaa ccttcccggg gaaatgatgc acgactcttt cgtctgcgga
     4801 tcgaggacgc ctactctact atcttctgga cggatgtgga gataaaggcc acggccacac
     4861 tcgaagtgtt agacgacttc ttgaggcaag aatggctgga atgctgcggc cacctcagtg
     4921 ctttctacat cgatgaggac acttacatgg ttccctccgc ctacaggatg gagcagttcg
     4981 ggttgttcgc agatcccaac gaacgcagca tgaaggccaa gctcggcggg atcctgagca
     5041 aaggcagccg tttccagcac gtctacgact tcggcacttc cactgatctg aaactgcgtg
     5101 tggcagatga gcgccagggg cgcgtcggta gcgagcctgt gcgtctattg agcctcaacg
     5161 aggcgccggt gtgggagtgg gaggtttgcg gtgaagaggc aagctggata tgtaccttct
     5221 gcatttacga acggcagaac ccgttctatt gcgagcttca ctcagaggat cacgattgtg
     5281 aagagtctga gatgctgctt cccgtggtga actctccacg catgggcatg tgcggctata
     5341 ccggacctga ctaacccccg atccaccagc cacgctgcga tcgtctccga catgactcgc
     5401 cgtgtaaagt tcgtgtcgac ttaccgaagc tccgctatag gaggttgcgg gccacatcgc
     5461 tagatagcaa gtaagctacc aacgtaagct ggcgtgcgac cgttttctgg ttcctgttca
     5521 gaagtcttgc ccctccctca ggcaagcaaa ggacacctta tcgtccccaa cctcagcaag
     5581 gttagcccca tgatatatca tgcaagggct cagcctctac cctccgaagg cagcggcatg
     5641 tagcacgcct tcctcaccga tgagcgcgta gcccgagatc ccgccttctt cgaacctcac
     5701 atcccgcccg actcctaccg cctggtgttc ttcgtgcgcg gctttcgccg cagcctcgat
     5761 gaagctcctg acggccgggg ggtcgggagt accgtagggt ctatcgaggc cgacggattc
     5821 taatgcgtag cccgagagca gcttgcggaa gaccctggcg aacgtgcgcg ggcctgcgag
     5881 cacctcgagc agcacgaggc gctctccgat cgcgacgact acaccgctgg ttccctctgg
     5941 aaggtctcgt gcgagcttct cgaaagcgtc gagcctctcg gatagggggg accgggaggc
     6001 tgctgcgtgg aggcgggcct tacgacgtac ttccttccat acggcgccct ggtcgctcct
     6061 gtggcccctg ccacgcctca gggagtcgtg cacggacctc gtctcgaggc gcctgagcgc
     6121 gaggtgcggg ctgcctgtcc ctggggagaa cgcccgcgaa cggtagctcc accgcccgcg
     6181 ctcgatgcac gagactggca acgtgagatc ggagccggca gcaaccagaa cgctggagtt
     6241 caccacgcgg ttctgtttgg cgccgatgag cttgtctccc tccaggatca agactcgcag
     6301 gcttccctca ttcacgacgc gaagctcggg tacgctgccg gcttcgtgcg gctcctttat
     6361 gtgcaacgtt cctgcctgga gggcgccttc gaggagggag atatcctgct ccgcttgggt
     6421 gtcaccctgt agcggaaaca tgtgcaacag gccgtgggag acgggtcctc ttatctccag
     6481 gctttgcagt gcttgggcga gcgtctcgtc catgttgtgt gctccctttc gtaccgtctt
     6541 cgcggcaccc gttctcaaaa ctcaaatcag acctacaaac tatctatgct gaacgcatgg
     6601 ccccctgata cagcagtgtt cttagtggga tcccgatgcc caatacttga ggcctgtcgc
     6661 tgctgtttgc ggcaacccgc tctcagatgc caagcgacaa ctcccaaaag aactccggga
     6721 tggtcgcgat ggcctgccac acagagcctg cctcgatgac tcccaacagg accgcgacac
     6781 ccgaagcgat gatcagggga ccgccgatga gccccagcat ggccatggcc cgtggcacga
     6841 gggcggagcg gtacatcaag tagcccagaa tcagcccgtt cccgaggccg acgacgaagc
     6901 cgggtccgag cacgaacgtc cagtcatgga gcaccatgag cgactcgccg accgcgacga
     6961 gcgagccggc atccgcgccc gcagcctcct gacgcaatgt cacgaccgaa aggaggctga
     7021 ggaggcccac agcgatgaag gcgcattcaa cgatacgagc cgtgacatag cccagagcaa
     7081 ggatttcgtt ctgccgcttg acgacgggat agagcacaac ggcggtgccg atgttggcga
     7141 tgatgaggag cagctccagg aacgctccca ggagcacgcc gttgtcggcg ccagcaccga
     7201 cgatgtagcg agggtcgtcc agccgtcaag acgaaggtcg tagaattcgc ctactcacac
     7261 aaccgccaac gagaggaagg aaccaagcca tggaccagcc aaccgcagag gaggcgctga
     7321 agcggctcga gccgctggtc ggcgaatgga tcctcgaggc caagccgccg gacgggccgc
     7381 cattgccggg cgaggcacgg gccacgatcg aatggcatga ctcgggagca cacctggtac
     7441 agcgctcgac ggtggagatg cccgaggcac ccgactccat ctcgatcatg ggatgcgacg
     7501 cagccaacgg aacctacttc cagctctact ccgacgagcg cggcgtctgc cgcgtctacg
     7561 agatgagcat ctcagacggg aggtggaagc tctggcgcga gggcgaaccg ttcccgaagc
     7621 gcttcatcgc gacgatcagc gatgacggca agactatagc cggccgctgg gagaaggcgc
     7681 cggacggccg cacctgggag accgactttg actttgtata ccgtaaggtc aggtaagcgg
     7741 gtgtcttgca ccgaaacgct cgacggccaa agcgggggca cacctatcca agagctcagc
     7801 gcctggggca ccggcccttg tgacggaccg gggctatgcg cagttccgag acctcgctaa
     7861 agtcgaagtt cctaagaatt caccttccac gcactcgggg gaataggcgg gttcacgctc
     7921 attctttctc gcccaagaac atggctacca ccacccccga gaggaaggtg atcactccaa
     7981 cggagcctat ggcccacgaa agtcccaaag tgtcagcgat gatccctgca cagagcgcgc
     8041 ccacagcgta gccgaggtct cgccagaagc ggtagacgct caacgaacgg gcacgccagg
     8101 aggggtgcga gttgtcagag acggccgcga tcaaggatgg gtacaccatc gccgtgccga
     8161 ggccgaggag gatgctcccc cacaaccacc acccgaagct cctgttcgcc acggtgagga
     8221 agatccccca gcctgcaccc acatccccgc cacgatcaag ccctttcgac cccaccggtc
     8281 gctcaagggc ccggtcagga cctgaaggca cccccatacc gccgggtaga cggccttgag
     8341 cacaccgatg gcctccaccc cgagcccgaa ggccgagaag aacaggggga aaacgcccca
     8401 gctcatgccg tcgttgaggt tgttgaccag cccagcctgg gagcaggcga acaggtggcg
     8461 atccttgaag gaggtcctgg agaaaacttc gcggaaggtc agtggggcac tctcgggagg
     8521 gtgttgctcc atctccaccc tgacgtgttc ggtggtgtcg cgcacgagga agaccgagat
     8581 caggagcccg agcgtcacgt agccgatccc caggtagaac ggctctggcc tcaagccgta
     8641 taccgaggcg aggtagccgg tcgccagggc ggtcagtccc acagcgagat acccagcgaa
     8701 ctcgttgagg cccacggcga ggcccctccc gcgcggccct accaggtcta tcttcatgag
     8761 caccgtcatg gaccaagcga ggccctggtt gaccccgagc agtacgttgg ccgccacgat
     8821 ccacccccac gagggcgcca gcgcgatcat gaacggcacc ggcacgccca cgacccaacc
     8881 cgcgacgagc accttcttcc tgcccacgcg atccgccaga gtgcccgata cgaggttgga
     8941 gcacgccttg accaccccga aggagatgat gaacgagaag atcgccgact tcagcaccag
     9001 accgaactgc tgggagccga tcagggggac cacggtccgc tcgaggccca ccatcccgcc
     9061 gacgaaggcg ttgacaacaa ccagtagcga gaactggctc aagttttccc tgagcccgag
     9121 ccgaactacg ggagcatgcc ccctatccac gccggggtgc cagagagcgc tctgtctcag
     9181 gcatggtggc gccgacgtaa cggaacccgg tagcgttgat ggttcgcgcc tctatggccg
     9241 cgctctcgtc cgcctcctcc gtgtcctccc cgagttccgg gtagaggtat ggcacccact
     9301 caaagaagcg ttctacgaag cggctcccga gttcgtcgcg catccgccgg gagctgtgga
     9361 acccctcctc gctagcccac tcggcgaagt acgattccgc gcgagccctc tctcctcctg
     9421 ggcccccacg cacccactcc tggcagcggc gccccaacca cgaaggcttc tctgacaagg
     9481 aggcgaccgg aaggcgctcg agggcccact ctgctgtggc ctcgtcgatc cgatcccagg
     9541 cgtactcgat cacgaccagg ctccctcccg ttcggagcat gtcagctatg ttgtccagtg
     9601 cccctccgag gttctcgatg tggtgcaggg acaggctcgc caccacgtgg tcgaacggat
     9661 ctggatctgt gaactcctcg atccctacgc gccggaagat gggaccttcg ggcgcctgag
     9721 gatctatggc cgtcacggag tgtccggcgc gagcaagggc gtgagccagc tctccctccc
     9781 cgcaaccaac ctccagcacc cgggcaggtg ggagaccaac ctggtccagg acgaactcct
     9841 cgacgcgctg ctgccacatc gcgagctccc ccatgagacc tcccttctta cgcgaagcgc
     9901 tctctagagc ctcgcggacg acccgccggg atcaccctat ccgatgctct ggagacactc
     9961 aagctgactt tctacacacg aactctttgg aactccgtaa aacggaagtc caacttcgga
    10021 gaatctacct tccaggcact tcagtgaata gcctcgagag tacggctgct acgcgccgcc
    10081 cagggcattg cggtaaacca actctgccgt agccacgtcc tccaccgcca gtccgagcga
    10141 cttgaacagc gtcacctcct ccaaggatcg gcgaccttgc tccgctccag agaccacctc
    10201 gccgatctcg gcgagtacct ccctggctgc gatcacgtct ccgctctcct tcatagcggc
    10261 ctcccgcgag tccacgtaga ccttggcgcg gcgcagcacc tcgtcgtcga gctccctcca
    10321 gtcggggcgg ggcgcgccaa ccgcgttgat gtgcgccccc ggcgagagcc actccccgta
    10381 tagcaccggc gtcggtgagg tggtggccgt caccaccacg tcggcccccc gcaccgcctc
    10441 ctccgccgag gctgcggcat caactccatg ctcctcggcg aacgcggccg cccgtcgcgg
    10501 gctccacacc ctgacctccc gaaactcgcg caccagcctc agcgcctcaa ggtggcttcg
    10561 cgcctgcact cccgagccga tgatcgccag caccgaagcg tcggctcggg cgagatgatc
    10621 tgttgcgact gccgaaactg cagcggtgcg cacctcggtg atgagccgac cgtccatggt
    10681 cacgaggggc tcgccggttt cgggttcgaa gaggaggatc gtggcgtagt gggttggtac
    10741 ggaggtgttg cgggggtaga aggctactag cttcgcaccc agagctgatc ctgtgtaggc
    10801 gggcatgagg ccgaggaacc ccccgtgctc ggcaacgggg agcatcgtcc gtaccggttg
    10861 caccaccgta ccgttagaga agtcggccag cgcccgctcc atggctggga tcacctcctc
    10921 catgcgcagc agacggccga cggcgctctc ctccaggttc atccccgcac acctcccctt
    10981 taggacacta ccgtcgatgg tagtggacaa ccaagccagg cgcaccctcg acaccccgcg
    11041 agctccgaac ttcttccgcg aacctgactt ccacgacgtt cgtgaacccg cttttctctg
    11101 gcgaatccta cccctttcca ctacgagagc atgatgccac caggccaagt ccaacgcgaa
    11161 tatcggagaa tgctccccta cctttatgtg tcggtcaggc acgtatcctg gatgacgccc
    11221 atgggtgccg aggatggctt ggtggaggct catgaccccc actacgagat gttcggcttc
    11281 cctcggatca atcggcggga atcatgatgc catgattgcc gtctactctc tgaggcagac
    11341 cgtcgctgat catgtagtaa tcgcccgaat cctcgacgaa cacgtgaacc gccgtctcga
    11401 gccccgtcgg taggtccaac gtgccagccg cgatcgaaac ccgctcgccg gaagcaggct
    11461 cccaaaacag gctagatccg caatatccac agaacccccg tcgtgctttc gctgacgctc
    11521 ggtaccagcg tagtcccccc gactcgatca ggaccaggtc ttccgggcgc gtcgccgtga
    11581 aggccgcgaa atggccactg gtacgccgac acatcgtgca gtggcaatcg accacgggac
    11641 atagcggtcc acgcacttcg tatcggacgg cgccgcagag gcagccaccg gtccccctga
    11701 tccgaggtga atcttcttct cgcgtcatcc tcctacgacc gatccacaag aacgcttgat
    11761 cccacagcat gatgtccgac ttcggccttc tccgtcaaaa cgagactcct ccttcgtgtg
    11821 cgtttcacat gatacagcga acctccggct ggcacctcca cgcctcgatg acctgccgac
    11881 ggagccttct ccaggacatc cacagagccg agaatcaggc ggataactta cgttcggacc
    11941 ttgtgcagca gtcgcacggc aacctctgga tggtgggtgc caaagctcaa ctggtgagct
    12001 ccatgagagt aggctgatat tctagtgttc ggcaccagcc gtcaaaggta tcgacggtct
    12061 ccatagacga gtgaggtgca gcactccgct tgtgagaatt ccggccacgt gaggttcggg
    12121 aagcgggata ttatcccatc tccacgaagg tgaactattg cctgccgcca gtaggtctct
    12181 gatcacagtg gtttgttttc tggtcgccac cacttccacg tagtccgact gtaccaccat
    12241 cgtctttcga acggttgtag gattgatcaa gtcgatcaga tcgccttcca gactttcttg
    12301 tccgtctgcg tcgacagctt cgaacgctgt gaccatccca ctcttgatgg tgacggcatc
    12361 ggtcgaacga ccccgccagc gtttcccgct tccacctgcg ctgagtgcct ccatcaatcc
    12421 tccagtcggt cgcagacgta gtcgcgggag gggtcgtagt cgcgtccgtc tatctcgcca
    12481 agccggggac cgtaaagatg cacagagata gccggctctc cggtcgggtt tcccacccgg
    12541 tggatgcccc cctcatatgg gagcacggtc gagatcccat catcccttct ccactcgagc
    12601 cgccagagct ccttcaggcg cgcatgatcg ggcatggagc cgtcgtccgc acgctcgtag
    12661 cgctcttcca ggacggatcc cacgacgcaa cagaatgctc cccaggagga atggtcgtgg
    12721 atggcggtcg cggaacccgg aggccacacg aagatctgca gcgagcagta gcggtcgggg
    12781 tcctcgcggc gataggccac gtaccagtcc tcggcccttc ccgcttcctc cagatgaggc
    12841 aggacgtgcg aatccagaaa ggctggatcc ctcacaaggc gggagagagt ggggatggca
    12901 tcctgcaggg tcggctccgg aagaaccccg gggttgctcg tgtttggcat caacatcatc
    12961 tttctcctct cgcgtctgcg gtgtactcac acaataggag gaggcgaggg aacgacgcat
    13021 cggggtaacc acccaacttc cgtaaaatgg cggagtgggt aattccaccc acgagagctt
    13081 gctcagatta gttggtgctc agacgagctg gtgctcgtac gcgtaggccg tggccgcggc
    13141 gcgtgaggac acgcccagtt tggtgaagat gttgctcacg tgccggtcca cagtcctgtc
    13201 gctaaggacc agctcggcgg cgatggcctt gtttgtctcg ccagcggcga ccaggcgcaa
    13261 cacttgcagc tcacgcggcg tcaacccatg agcatcgacg gacggggcgt ccctggtgag
    13321 tgagtcgact ttggtgagat ccggcgttgc tcccagctgt tcaaagacgc cgcgggccgc
    13381 ttccagctcc aacgcggccg tgtcgtcgtc gcccagcgcg cggcatgcct gtcccaggag
    13441 aacgcgcacg cgtgcgacct cgtacggtac ctcgagctcc tgccacacct gccacgcgtg
    13501 acgcagcgct ggtagagccg cccgcgcgtc accctgagtc agggccactg ctccctcggc
    13561 gtgcgccacc atcgcactca gcattccgct cttgtactgg gccaggactt cctcgagttc
    13621 gcggcaggcg ctacgtgcct cctcggcttc gcccatggcc agcatgatct cgacgtaggc
    13681 gggcagcagc ctggcacgct tcagtggctc ggtcgtctcg cccacaaccc ggcggatcgc
    13741 agcggacgcc gcatcgccgt tcccctgggc caaccgcaac agggccagac ccggctgagg
    13801 ttcgcacccg caccggctcg cgtcccggta tgcctcctcg gctgcggaga actcccctcg
    13861 caggcgatgg acctcccctt gcctgtagaa cgcttgagcg gccgccggct ggtttttccc
    13921 ctgcacacta cgctggtgag cccgccgcgc ctcttccagt gcctcccgcc acgcgccatg
    13981 cagttccatg atctcagcgc gatgcaccag gcaccggccg ctgaaggcca ccatctcggg
    14041 ctgctgctcg caccactgtg tcaaggcggc ggtccactcc tgggcgcgat gcagcgcata
    14101 cacttcctga cagcccatga tcacgctgca atagaccata ccggtgacga tgggcgacag
    14161 ctcccccgcg gtgaccgcca ccatagcttc gtcaaatagc ccgaggccct cctcgaccct
    14221 cccctgcttg accagggcgt ggccttgctc gtgcacggcg agcgcgagca ggtcttgatc
    14281 accgaagcgc tcgccgatct cggcagcatc ggctgccgta gcgtaagcgg cctcccagtc
    14341 accgctggcc tcatgccgca acatgaccgg caccagcaga tagccccgct cgacgcggtc
    14401 gcttccctcg cgttcgacca gccgctgagc gcggccgagc cagcccgtcg cccggcccat
    14461 ctcgcctcgg agtgcaaggt tcatgcccac ccagaacgcg cagcgcacgg cgcgcatcgc
    14521 ttcaccaccg tccaggtaca cgtgataggc gcgctccagg ccgctcaggc actcgtcgtc
    14581 gcggccgagc atgaaggcag atgttgcgag cagttcgagg tcctccgctc cgagcggcgc
    14641 cgtccgatcg gcgcgtgaga gcgactcgta ggcagccatc cacgcccgcc tcgcgtagta
    14701 ctcacggccc cgtccgaggt cctcgatggc ttccgccacg gctactccaa ccgctccggt
    14761 gcagtgtctc ggcccactgc gcaccccaag taggagccgg gacctcgatg gtacctccac
    14821 ccggtgtcgc atagcacacc tgatccgggt gctctgctcg catggctcgt gtggtccggc
    14881 gagtccccac ctcccgcctc gtcaaaggac cgaaccaccc gaacccgctc cattcacctt
    14941 ccccggaacc ccacgaatca tgttccttgg agccaattat agcctgcgta tcaaccgaac
    15001 cacatccagg agctgggttc gcttgcagtc atcagggcag gtcggatcag agataacggc
    15061 tcggttagag atctcgtcgt tagtttgcag gaattgtgca gattccagaa gggcggtgtc
    15121 ctccgttgcg ccagacactg cggttaggac gatgggacgt cgactgctcc tttgcactca
    15181 aacgcaaggt cagggtcaag gctgatctta tgacatctgt aacctcgaga ttacgacaac
    15241 cttcactact gagcaaagag tatcgaccat agacttgctt acgaatagag tattcggcga
    15301 aggagaccgt agagatgcta ttcggagaag ccaaagagga agtaggttcg gggtctgcgg
    15361 gcgacgccat ggtcgaggcc aatgacctgg agaagaccta cgcggccggt ggtgcaacgg
    15421 tacatgcctt gaggggcgtg gacctgagca taccccgcag cgagatggta gcgatcatgg
    15481 gcccttccgg ctgcggcaag accacgctgc tcaactgcct ctctggtctc gacgagttcg
    15541 acagcggcga ggtcttcgtg ggcggtgagt ctatctccgg gatgtcggac aggaggcgca
    15601 cccgtttcag agcggagcag atggggttcg tcttccagac ctacaacctc atcccagtcc
    15661 tctcgggcat cgagaatgtg gagctaccgc tcctggtcgc cggagtaagg tcgaaggagg
    15721 cgcgcgggcg ggcgctttca gccctggaga tggtcgggct cgcagaccaa gctaacaaac
    15781 gcccgaacga gatgagtggc ggccagcagc agcgggtcac ggtggcccgc tctctggtca
    15841 acgaaccgtc aatcgtatgg gccgacgagc ctaccggggc gctcgactcg gagaccagcg
    15901 cggagatcat ggatctgctc gccaagctaa accaggagaa aggacagacg ttcgttttgg
    15961 tgacgcacga catctccgtt gctcgtcgcg ccagccgcct cgtcaccatg cgtgacggca
    16021 ggatccagag cgacgaaccc gccgtcaagg gggagcgcga tgtttgagtt cctcggtgta
    16081 cctgctgagc gcctgacgct gatcctctcc ggcgtcttcg cggcgggggc tctgaccctc
    16141 gccttccttg ccgcgcgcga ccggacctcc ttcaggatgg cggtgaggaa cataccgcgc
    16201 cgcccaacgc agaccgccct gatcttgctg ggactcatgc tggcgaccct cctcttctcg
    16261 gcggcgttca ccaccggtaa tactctgacc aactcccttc gcacccaggc gctcgaaaac
    16321 atcgggcggg tagacgtggt agtcaaggcc gagcagccag agtcgggcag cgttgcgttc
    16381 ggacctggcg ccggaggggc accggacctc gagacgcgcg agaggtactt cgacgaatca
    16441 ctcgtcggca agatccgggg ccacctctcc gaggtcgaga gcgtcgcggg cgtcgcgccg
    16501 ctcgccaagg agagcgtgcc cgtgacgtcg ccgaagacgg atctcagcga gccacaggtg
    16561 gacgtgcttg gtatggatgc aaactccatg cggggcttcg accgcctcag aactgcgtcg
    16621 ggcacgcccc tcggtctcgg ggatctcgag aagaacgagg tgtacgtcgg gacagagaca
    16681 gcagaggggc ttggcatcgg ggtaggagac agggtcgagg tgtccctgat ccaacccccc
    16741 tcgaagccgc aaagcaaaca gcgcctcggg acggcgcaag gacgtcccag agggccggag
    16801 gcccgcatgc ctcctgcggg ggcgcagagc ggagagcgtc ctccagggtc gaccgaagac
    16861 gcgcgtccag ccgggtcgga ggtcgagacg cgccctgccg gggcgaagag caaggaacgc
    16921 cccccagagt tcgcggtcgc cggcgtctac gagagcggag ccaaccccgc ttccgacacg
    16981 tcgatggtga tgccgctcac gcagatgcag agactcaccg gcgagggtgg caaggtcaac
    17041 gaaatcctta taacgcacgg cggacccgcc acggagggcg gtgagcacac gagcaagacc
    17101 gtcgacaaga ttcggcctgt agtttcggcg aactctctgg aggcggatcc ggtgaagaag
    17161 gaggccatag aagcggccga ccagagggga gagaccttct ccgccctctt cgtgctcttc
    17221 ggacagttct cggtggcagc gggcatgctc ttgatcttcc tgatcttcgt gatgctcgcc
    17281 gccgaacgca agagggaact cggcatggca cgcgccgtcg ggatgcagcg ctccaatctc
    17341 gtccgcacct tcgccttcga gggagcgctc tacgccctcg cggctagcgc tataggcagc
    17401 gttgccggtg tcggggtggg ctgggtcatg gttcgccttg tgggtagagt actcgccggc
    17461 ggggaagggg atttccggat cgcctttgcc agcagccccg agaacgtcgt cctcgccttc
    17521 tgcatgggga tggttctgac cttcgccgtc gtgctgatct cgtcctggcg cgtgagccgc
    17581 ctgaacgtcg tgcgcgccgt ccgcgacctc cccgaacccg accgtagagg ccgcagtgtc
    17641 tggggagtgc tcgtggcagt caccttgcca ctcgcgggga gcgtggcgtt ctggcaaggg
    17701 ctcgcgaccc agacgatggc cttctacctg ggcgggatct cactcctgct catcggagcg
    17761 gcgctcctgg cacgtgtcct cggcctgccc gatcgtgtcg ccttcacggc ttcagggctt
    17821 ctccttctgg ccctgtggct ggtcccggcc cggatcaccg ctcccgcggg gatggccaag
    17881 ggaccggaga tgttcttcgt ctccggtatc gccatcgtcg tcgcgggggt gtggctcgtg
    17941 atcttcaaca ccgacgtgct cttgtggatc gtcgtggcga tcttcggacg ggccaaaggc
    18001 ctcccaccag ttctcaaaac agctgtcaag tacccgacgc agagcctctt cagaaccggc
    18061 atgacgctgg cgatgttcat gctggtcgtc tttaccctga cggccatgaa cttcatacag
    18121 gccgcaatga gcgctgcctt cgaggatacg caggcgcttt ccggcggcta cgaagtacag
    18181 gccgacgcca gctacgcttc cccaatcccc gacctggccg cagccatcga ggaggcggac
    18241 ggtataaaga gtggcgatgt gagggccgtg ggggaggtat ctaacctccc gctcgaagtc
    18301 aagcagaggg gcacaaaccg agacccgagg ccgctctacg tacagggcgt ggacgggggc
    18361 tactctgaca gcgtcgacta cggcttcgag acgaccgcca gaggttacgg ctccgcccgc
    18421 gaggtgtgga aggccctaca aaccgagaag gacaccgcgg tgatctccag cggcctcgca
    18481 ccggccagga acgtctccac cttcggttcc tcagccgagc cgcccgtgag actcaccggc
    18541 ttctatgccg acgataagaa cctgcctgaa gacctctacc tccggataga agaccccgag
    18601 tcgggccaga cgagaaacct gcgcgtgatc ggggtcctcg gaagctcggc ctcctttgcg
    18661 ggggacgtcg tgacctccca gggtgcgctc gaaggactgg ctggccgtcc ggtggcgtct
    18721 cagagatact atttcgacct cagtagcgga gtcgacgccg caattacggc caagaccctg
    18781 gagaaagact tcgccaagaa cggcctgcag acgcaggtta cagccgaggt gatccgggac
    18841 aatgacgcga cgcggaggat cgtcttcttc ctactccggg gtttcatgag tctcgggctc
    18901 gtagtgggca ttgccgcact cggggtcata gcggcccgct cagtggtcga gcgacgccag
    18961 cagatcggga tgatgcgcgc tctaggcttt cagaggcggc aggtacagct cgtgttcctg
    19021 accgagtcgt cctttatcgc cctgctcggc attggtgtgg gggtggctct tggcctcggt
    19081 ttctcgggca cgctcataga caatatcagc cagaatatcc ccggtctcga gtacaccgtc
    19141 ccgtggggcg cgctgctgct cgtaatgatc ttcggctacg ccgcctcgct ccttaccacc
    19201 tacttgcccg cccggcaggc gtcgaaggta tacccggccg aagccctgcg ctacgagtag
    19261 catgtgggta cttcttcagg gcgcctgcgg cgtggttcat cccggccggg cggttctccg
    19321 ggctgctggt cagcggcatc gccggtgccc tgctccgctg ttactcaggc taatgagcgc
    19381 gaagcgctcg tcgggacctc ggctcgggtc ccggcgtcgt aaaatcgagg aggaaatatg
    19441 acccaaacta ggcccggaag cgaagtcaca gcgccggcag gatccatcga ccatgaggtg
    19501 atgagcgtgt cacagaaccc gacagcggcg gcacaggaga ggaacgaggg gccaccgaca
    19561 ggatctgcca acacgtccaa gatgggctcc gacacctcgc tgcgcaacct gctccccctg
    19621 gcgtggccgc tctttgcgct gatgtggttg gtgttccccc tcgcatccgt ggtcgacatg
    19681 ctggggaagg atctcgcgcc gatccgactt ctcgcgttcc tggccttgac ggtggcctac
    19741 gtcgcggtct atttgtggct cgtgctgcgc tacccattcc gcagcggcga gatgtcgccc
    19801 tcagagcggc ggttccacgt cgcgctcctt cttgtcctcg cagccctcgc actgtgcctc
    19861 gaggtcgcgt tcggtagcgg catcccttac cagttcatgt tcgtcgtgat cgccgccgcg
    19921 gtgacactcc cgacgatacc tgcggtgtcg acggtcatcg ccctcatagt cctgactagc
    19981 gtcatatatg cactccgctc ggggtgggat acgcttgcgt cgtc
//
LOCUS       DASTKM010000160         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_570402, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000160 DASTKM010000000
VERSION     DASTKM010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570402"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..606)
                     /locus_tag="VFQ10_06710"
     CDS             complement(145..606)
                     /locus_tag="VFQ10_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9965068.1"
                     /translation="MKIFPTHILLATDGSPHADLAAEVAIDLVQSTGSRLHVVAVGRT
                     FPAAVYDEYAEARRDDLRREAQEILDEQVRKIEEAGGTVAVAHLKMDEQRDEAIVHLA
                     EDIDAGMIVIGSRGFGGMKRALMGNVADSVVRHAHCPVLVVRPTGGASRPF"
     gene            complement(634..1371)
                     /locus_tag="VFQ10_06715"
     CDS             complement(634..1371)
                     /locus_tag="VFQ10_06715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9965069.1"
                     /translation="MRNKVAVVTGASSGIGATVARALNAEGAHVALAARREEALLEIQ
                     ADLDSGDGRKCMVFPTDVTDREEVRSLVARAEEELGPVEILVNCAGVMYYSLMKNQRA
                     EEWERTVEVNCKGALNCVGAVLSGMLERGRGHIVTISSDAGRKVFPGLAVYSASKFFV
                     EALSQGLRLETAGMGVKVTTIQPGNVATDLIEMSGDEEALEEYGQPGESRVLDPEDVA
                     ASVVHALVQPEHVAVNEILVEPRDEPI"
     gene            complement(1537..2112)
                     /gene="cysC"
                     /locus_tag="VFQ10_06720"
     CDS             complement(1537..2112)
                     /gene="cysC"
                     /locus_tag="VFQ10_06720"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET9965070.1"
                     /translation="MNEQRGFTLWFTGLSGSGKTTISEIVEHELRERERQVEVLDGDI
                     VRTNLSKGLTFSRDDRNINVLRIGFVANLLTRNGVGVIVSAISPYKEARDQVRRRIVD
                     FVEVFVDAPLEVCAERDVKGLYKKAFAGEIEQFTGVSDPYEPPNAPDLVLKTDEESPQ
                     ESARKVIEKLEFFGYLWPREAEETEYNWSSR"
     gene            complement(2176..>2715)
                     /locus_tag="VFQ10_06725"
     CDS             complement(2176..>2715)
                     /locus_tag="VFQ10_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HET9965071.1"
                     /translation="KASDALVSHIDLFPTVCDLAGVERPEWLQGESLLPLANGEADEV
                     RDAIFAEKTYHVAYEPERCVRTHRWKYIRRFGDRTKPVLPNTDDGPSKELLLSYGWGE
                     REIPPEQLYDLVLDPNEAHNLAGDPAHSVTLEELREKLKAWMVETKDPLLEGDVPAPE
                     GAELNDPDGLSPNEPTIVT"
BASE COUNT          453 a          919 c          785 g          558 t
ORIGIN      
        1 acctggcggc tcgtcgagct acctacaggt cactggccca tggtctacag gcccgaggac
       61 ctcgccactt tgcttctcga ccttggctga aatgctgagg agccgcctgc aaggtggcgt
      121 gctgacaagc tgccgtaggc agcgctagaa cggccggctg gcaccccctg tcggtcgcac
      181 caccaggacc gggcagtggg cgtgacgtac aaccgagtcg gcgacgttac ccatgagggc
      241 gcgtttcatc ccgccgaacc cccggctgcc gatcactatc atgccggcgt cgatgtcctc
      301 ggcaaggtgt actatcgcct catccctttg ttcatccatc ttcaggtgcg cgacagcgac
      361 ggtgccgccc gcctcctcga tcttcctgac ctgttcgtct agtatctcct gggcctccct
      421 cctgagatca tcgcgccgcg cctcggcata ttcgtcgtag acggcggcag gaaacgtgcg
      481 cccgaccgca acgacgtgca atcgcgagcc tgtgctctgg acgagatcta tcgctacctc
      541 agccgccaga tccgcgtgcg gagaaccgtc ggtggccagt aagatatgcg tggggaagat
      601 cttcaactcg ctgtcctttc gtaggagcgt tcctcagatc ggctcgtcgc ggggctcgac
      661 cagaatctcg tttacagcga cgtgctctgg ctggaccagc gcgtgtacca ccgacgcggc
      721 cacgtcctca ggatcgagca ccctgctctc gccaggctgt ccgtactcct ccagcgcctc
      781 ctcatccccg ctcatctcga tgaggtccgt cgccacgttg cctggctgga tggtcgtcac
      841 cttcaccccc atgcccgcgg tctccagtct caggccctgg gagagcgcct ccacgaagaa
      901 cttgctcgcc gagtagaccg caagaccggg aaagaccttc cttccagcgt ccgaagagat
      961 cgtgacgatg tgcccccgcc ctctctccag cattccggac aacaccgcgc ctacgcagtt
     1021 gagcgcccct ttgcagttga cctccacggt acgctcccac tcctctgcgc gttggttctt
     1081 catcagcgag tagtacatga ctccggcgca gttgacgagg atctccacgg ggccaagctc
     1141 ctcctcggcg cgcgccacga gcgaacggac ctcctcgcgg tccgtaacat cggtcggaaa
     1201 gaccatgcac ttccggccgt caccgctgtc gagatcagcc tggatctcca aaagcgcttc
     1261 ttcccggcgc gccgcgagcg ccacgtgtgc gccctccgcg ttcagcgccc tggccacggt
     1321 ggccccgatg ccgctcgagg ccccggtcac cactgcaacc ttgttccgca aagatcccac
     1381 ctcctcgcgg aaactaccct ctcacagcgc tagtcacaag aatcgttcct gcacgcccaa
     1441 agagctgatg tgctgacaag cgaaggcgaa gcctgagcgt gccgaaatgc tgaccagcgc
     1501 ggcattggga aggaccagtc cttcccaatg ccgctatcag cgagagctcc agttgtactc
     1561 cgtctcctcc gcctctcgag gccagaggta cccgaagaac tcgagcttct ctatgacctt
     1621 gcgggcgctc tcttgcggcg actcctcatc ggtcttgagg acgaggtcgg gggcgtttgg
     1681 cggctcgtag gggtcggaga cgccggtgaa ctgctctatc tccccggcga aagccttctt
     1741 gtagaggccc ttgacgtccc tctctgcgca cacctccaga ggagcatcga cgaagacctc
     1801 gacgaagtct acgatcctgc gccgcacctg gtccctcgcc tctttgtacg gtgagatggc
     1861 gctgacgatc acgcctaccc cgttgcgcgt gagcaagttc gccacgaagc cgatcctgag
     1921 cacgtttatg ttgcggtcgt ccctggagaa ggtaaggcct ttggacaggt tggtccttac
     1981 gatgtccccg tcgaggacct ccacttgccg ctccctctcg cgcagctcgt gctcgacgat
     2041 ctcggagatg gtcgtcttgc cagacccgga tagaccggtg aaccataacg tgaatccgcg
     2101 ttgttcgttc atatacgttc ctttcgacgc tcccactgtc tccagccgta gtttacattt
     2161 ccctcacggc cctgctcacg tgacgatcgt gggctcgttg ggggagagcc catcagggtc
     2221 gttgagttcg gcgccttctg gcgcaggcac atctccttcg aggagcgggt ctttcgtctc
     2281 gaccatccaa gccttgagtt tttcacgcaa ttcctccagc gtcaccgagt gggcagggtc
     2341 cccggcgagg ttgtgcgcct cgttgggatc caggaccagg tcgtagagtt gctcgggggg
     2401 gatctcacgc tctccccatc catatgagag cagtaactcc ttgctcggtc cgtcgtcggt
     2461 gttcggtagg acgggcttcg tacggtcgcc gaagcgtcgg atgtacttcc agcggtgggt
     2521 gcggacgcac cgttcgggtt cgtaggccac gtggtaggtc ttctcggcga agatggcgtc
     2581 cctgacctca tcggcctccc cgttcgcgag cgggaggagc gactcgccct gtagccactc
     2641 gggcctctcg acgccggcga gatcacagac ggtcgggaag aggtctatgt gggagacgag
     2701 ggcgtcgctc gcctt
//
LOCUS       DASTKM010000161         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_219316, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000161 DASTKM010000000
VERSION     DASTKM010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219316"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..1035)
                     /locus_tag="VFQ10_06730"
     CDS             complement(421..1035)
                     /locus_tag="VFQ10_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9965072.1"
                     /translation="MPRRHGYMEWMLDFWGVEQADIVGHDIGGATAMIFTVNRPERLG
                     KLLLIDTVSYDSWPSETWQKIIRDHLHQYHRMTLEEFRQMMVRQLTMTVCRKERMSKE
                     VLEAYLAPLAGEIGKASFFTHQVNHYDSRYTEEITEDLKYLTMPVKILWGAEDEWQPV
                     SYAKRLATDIPGADLGIIQEAGHFLMEDAPEKVVTELRDFLYRS"
     gene            1619..2383
                     /locus_tag="VFQ10_06735"
     CDS             1619..2383
                     /locus_tag="VFQ10_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9965073.1"
                     /translation="MEIEEKVAVVTGAGSGIGRAVARRLAHEGAAVVVADVDEDAGAS
                     AVREIHSEGGQAALVLVDVTSETDVSRMVTFAEEHFGSLDVLVNNAGGVTEPYFPEGE
                     PDRWGRAIDLNLRGVMLSIHFGVRVMQRGGGGAIVNISSMGGIGFQPYDKPEYGAAKA
                     GVVRLTASLATLEESMGVRVNCICPGWVDTPASRRSRAEMIPEERERLVPPVLLQPEE
                     IAEAVVMFVEDDSMAGRVMIWQEGEPWQIVPLDAPY"
     gene            2579..3145
                     /locus_tag="VFQ10_06740"
     CDS             2579..3145
                     /locus_tag="VFQ10_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9965074.1"
                     /translation="MAKLIYLMIASLDGYAADEDGNFDWAVPDEEVHAFINDLDRSVG
                     TYLYGRRMYETMVGWETDPTLAEQSPLMRDFAQIWQAADKIVYSKTLEAVSTARTRIE
                     REFNPEAIRQMKALAGRDLIVGGPELAAQAFKAGLVDECHLFVAPMVVGGGKQSLPDD
                     IRLKLELLDEYRFASGMVYLCYRTRNTR"
BASE COUNT          664 a          952 c          990 g          671 t
ORIGIN      
        1 ccgtcgatat ctcagagtac actcaggagt ttggcagtgc ccttacgtcc gctggaaagc
       61 tggggcgtcc ggagtacatc gctctcgcgg ttgcctttcc cgcctgcgac gacgttggta
      121 tcaccggaca gatcctttag gctcacggag cactaagctc agggaagcgg tagcgattcg
      181 gcagcgtcga tgctgcgctg gccctggtat ccccagcgtg gggattttca atgctagacc
      241 tgggtgagat ttgacactag tttgacacta acccgttgct acaacccgca acagtgagca
      301 acaccgctca aaagaatttg gcttaactaa gcggattctg caacatgtag gaacatgctg
      361 aaacccgtcg ccccgcactt ctaatccgcc ggtcgcaggt tcgaatcctg ccgggcgcgc
      421 ttatgatctg tagaggaaat cccgtagctc tgtgaccact ttctcgggag cgtcctccat
      481 gaggaaatgg ccagcttcct ggataatgcc gagatcggct ccagggatgt ccgtggcaag
      541 cctcttcgcg taggagacgg gttgccattc gtcctctgcg ccccagagga tcttcacagg
      601 catcgtaagg tacttgaggt cttcagtgat ctcctcggtg tagcgggaat cgtagtggtt
      661 tacctggtgg gtgaagaaag aggctttgcc tatctcgcca gcgaggggcg cgaggtatgc
      721 ttcgagcact tccttgctca tgcgttcctt acggcaaacg gtcatcgtaa gttggcggac
      781 catcatctgc cggaattcct ccagggtcat gcgatggtac tggtggaggt gatcgcggat
      841 tatcttctgc catgtttctg aaggccatga atcgtagctt acggtatcga tcagcaggag
      901 cttaccgagc cgttctggcc tgtttacggt gaagatcatc gctgttgccc cacctatgtc
      961 gtgtcccacg atgtctgcct gctccacgcc ccaaaagtcc agcatccact ccatgtatcc
     1021 gtgcctgcgc ggcaaccgag gtgtctctct ccaaaggcct ctccgagcgg ccgtaaccta
     1081 gcaggtcgaa ggcgagaagc cggtagccct cgctctccag aggcggaata acctcacgcc
     1141 agatgtatga atgcgaaggt gtaccgtgga cgagcaccac cggtcgtccg tctccttcgc
     1201 catcgcggta ggcgattcgc accccgtcca caaccacgtc ctgatctacg accgttttca
     1261 tctctgactc ctatttagac ggcatgcgag ctgatgttag ccactcgtat gcatcgcgtc
     1321 taacgaaaat atgagatgct accgatctcg aaatgagatg agaggagaca caggtggaac
     1381 tacggcacct gaagaccttc caggcggtag cgagtgcttc gagcttcacg cgggctgctg
     1441 cggaactcgg ctctcccctg tactcggagc attcatggag gttgccaggc gaacgctcac
     1501 acacgtgcca gatgcgcgcc aagacaagtc acccggcagc aaagcaacac gccaggaagt
     1561 ggaagcgaat tcaccggcat gatgaggcga cgagtcacgc taagggagcg aaggcgtagt
     1621 ggagattgaa gagaaagtcg ccgtggttac cggcgcgggt tccgggatag gtcgagcggt
     1681 cgcccggcga ttggcccacg agggggcagc cgtcgtcgtc gcggatgtgg acgaagacgc
     1741 tggcgcaagc gcagttcgtg agatccactc cgaaggagga caagcggctc tcgtcctggt
     1801 cgacgtcact tccgagaccg atgtgtcgag gatggtcacc tttgccgaag agcacttcgg
     1861 ttctctggac gtgctggtca acaacgccgg cggcgttacg gagccctact ttccggaggg
     1921 cgagcccgat cggtggggac gcgccatcga cctgaaccta cgcggcgtga tgttgagcat
     1981 acacttcggt gttcgcgtca tgcaaagagg cgggggcggc gcgatcgtga acatctcatc
     2041 catgggtggg atcggtttcc agccctacga caagcccgag tacggcgcgg ccaaagccgg
     2101 ggtggtgcgt ctgaccgcct cgctcgctac cctcgaggaa agtatgggag ttcgcgtcaa
     2161 ctgcatctgt ccgggatggg tggacacccc ggcttcccga cgaagtcgcg ccgagatgat
     2221 acccgaagag cgcgaaaggt tggtccctcc tgttctgctt cagcccgaag agatcgccga
     2281 agccgtcgtg atgtttgtcg aggacgattc gatggccggg cgagtgatga tctggcaaga
     2341 aggagagccc tggcagatcg tgcctctcga cgcaccgtat tgagaagcta ggtgaaaggg
     2401 cttttgattg cctcggcgtc ttccaggttc gtatccgtcc tggccgacat cccggtcgcc
     2461 tttcttccga gcttcataga cctctccctc tacggcgaag ggctagcggt gaaagtcacc
     2521 cgtctttcag ttacaaacct agcaccatgc aatcgggtag cgtaaagaga ggttcaccat
     2581 ggctaagctg atctacttga tgatcgcgtc gctcgacggc tacgcagcag atgaggacgg
     2641 caatttcgac tgggccgtcc ctgacgagga ggtgcatgcc tttatcaacg acctggatcg
     2701 gtcggtcggc acttacctgt acgggcgccg gatgtacgaa acgatggtcg gctgggaaac
     2761 cgaccccact ctcgccgagc agtcgcccct gatgcgggac ttcgcccaga tctggcaagc
     2821 cgccgacaag atcgtgtact ccaagacgct tgaggcggta tctaccgcca ggacgcggat
     2881 cgagcgggaa ttcaacccgg aagcgatccg gcagatgaag gccctggcgg gacgagacct
     2941 catcgtgggc gggcccgagc tcgccgccca ggcgttcaag gccgggttgg tggacgagtg
     3001 ccacctgttc gtcgcaccga tggtggtggg aggcggcaag cagtccctgc ccgacgacat
     3061 ccgcctgaag ctcgaactgc tggacgaata ccgtttcgct agcggcatgg tttacctttg
     3121 ctaccgcacc aggaacacgc gatgagcgtt ccagccgcct ccacttcctg cccatgcttc
     3181 ccccttcaga gatagctttg tgggacagga cgaatgccac gtccagggct ctccggccag
     3241 ccggggagtt cgggaccctg gaatagatct gccacac
//
LOCUS       DASTKM010000162         4749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_219517, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000162 DASTKM010000000
VERSION     DASTKM010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219517"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VFQ10_06745"
     CDS             <1..1128
                     /locus_tag="VFQ10_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET9965075.1"
                     /translation="TGMRTWPTRPPSFGWKPIPSRQTTVAEILSKEGFGTYLVTDTYV
                     QFPMNFGRGIGAYNMIRGQERDAYKDPSTISEEEMRRHYIILGEGKKARQYLANVRGR
                     EGEEDWFAPRVFLDAMDALEVAAGENQPFFLVADCFDPHEPWDPPEEYVSLYDEGYRG
                     KEPLDDNYGSDDYLTDRQLLRMRALYAAEVTMMDRWLGRFIDRAHELGLMEKTLFVVV
                     SDHGHLLGEHGYTGKLAYALYPELTDIVLMVRNPHGEGAGKSSDFYASTYDVAPTILG
                     FLGVEQPKPMDGQDLAPILEGKEPLRPRDHFTQGYKNYVCCRDEHRVMFCRSDMTDVH
                     LFDAVNDVDQRRDLAKAEPETVKRMYEDYAQKDAAGRLPNF"
     gene            1431..3371
                     /gene="sat"
                     /locus_tag="VFQ10_06750"
     CDS             1431..3371
                     /gene="sat"
                     /locus_tag="VFQ10_06750"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HET9965076.1"
                     /translation="MTQSKPVALWAVPRSISTAFERVFVERDDFEVLHEPFSASYYYG
                     EDRLSDRFVDAEPKAEYSYERVLEDVFRPREQRVFVKDMAYHAKKVLSTEFASRFVNT
                     FIVRDPKYVLVSLYKMWPDFTLEETGYEDLYRLFRYATDAGEDVAVVDAMTFSENPAG
                     VLAAYCEHLEVPFRSGSLTWESGEVSEWDSWEGWHEDAESSTGIEPAERLDPELPEEL
                     EAVYEQCLPYYYELAAHAIPSVARRSVPGRTDDKEKILLRTESAIAPHGGELIDRVVP
                     EAERRERSMEAAELPKVPLSPRALSDLQMISTGVFSPLEGFMLREEYEGVVEDMRLKD
                     GLAWSLPVTLSVDEEKARALSDNSEIALVDGTGEPVATMLLRELYGYDKEREASMVYR
                     TTDADHPGVAAVYRQGDVLLGGEVELLRPPDEGRFPRYYYTPSQLRATFAEKGWKRIV
                     GFQTRNPVHRAHEYIQKSALETVDGLLLNPLVGETKSDDIPADVRMRSYETILDRYYP
                     EDRTMLTVFPAAMRYAGPREAIFHAICRKNYGCSHFIVGRDHAGVGSYYGTYDAQHIF
                     EEFAPEELGITPLFFEHAFFCTECGGMGSSKTCPHDPDSHVFLSGTKVREMLSRGEYP
                     PPEFSRPEVIEVLMDGQRVESR"
     gene            complement(3398..3709)
                     /locus_tag="VFQ10_06755"
     CDS             complement(3398..3709)
                     /locus_tag="VFQ10_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965077.1"
                     /translation="MTGFLYRSGANGRVHGKGSAARGRLGPTLGSVVLASLLLCEVAA
                     AMVGAEAVFFSTLVAAWLVLPAVVGLRNVLDAGCIYMLISLAIVVVVSVASHCAGLRL
                     A"
     gene            complement(3706..3870)
                     /locus_tag="VFQ10_06760"
     CDS             complement(3706..3870)
                     /locus_tag="VFQ10_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965078.1"
                     /translation="MKWDLDLWYELWDRAGSQGLDTWYDLRGEARDGPDTGHAPDAGS
                     EIIEDGREGT"
     gene            4106..>4749
                     /locus_tag="VFQ10_06765"
     CDS             4106..>4749
                     /locus_tag="VFQ10_06765"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HET9965079.1"
                     /translation="MGTRSGLADPVLSYLICATPRSGSTLLCAALDDTGIAGHPEEHF
                     EVLLETGQPRQPRDYFQRSNDPEVWALLDDPEFRDVLGEYGGRYSEHPARRDPLWRPP
                     DFERLAEGALHKGTTENNVLGTKIMWAYFRDFVRLARRTTAPDVRPCEVPASVLPNLR
                     RFVWMRRRDTTRQAVSLWKALQTQHWRRDSDEDVGGQGLRFSFAAIDHLKLRID"
BASE COUNT          978 a         1449 c         1475 g          847 t
ORIGIN      
        1 acagggatga ggacctggcc aacccgaccg ccttccttcg ggtggaagcc gatcccctcc
       61 aggcagacca cggtggcgga gatactgtcg aaagagggct tcggcaccta cctggtgacc
      121 gacacctatg tacagttccc catgaacttc ggccggggaa tcggggctta caacatgatc
      181 cggggccagg agagggacgc ctacaaagac ccctccacta tctccgagga ggagatgcga
      241 cgccactaca tcatcctcgg ggagggcaag aaagctcgcc agtacctggc caacgtcagg
      301 ggccgcgagg gcgaggagga ctggttcgcc cccagggtgt tcctcgacgc catggacgcg
      361 ctcgaggtgg ccgcggggga gaaccagcct ttcttcctgg tggcggactg cttcgacccc
      421 cacgagccct gggatccgcc ggaggagtac gtgagcctct acgacgaggg ctacaggggt
      481 aaggagcccc tcgacgacaa ctacggcagc gacgactacc tcaccgaccg ccagctcctc
      541 aggatgaggg cgctctacgc cgccgaggtc accatgatgg accgttggct cgggagattt
      601 atcgataggg cgcacgagct cgggttgatg gagaagaccc tgttcgtggt cgtctccgac
      661 cacggccacc ttctcgggga gcacggctac acgggtaagc tcgcctacgc cctctacccc
      721 gagctgacag atatagtcct catggtgagg aacccacatg gcgagggtgc tggcaagagc
      781 agcgacttct acgcttctac ctacgacgtc gcgcccacga tccttggctt cctcggagtg
      841 gagcaaccca agcctatgga cggtcaggac ctggcgccca tcctggaggg taaggagccc
      901 ctgcggccga gggaccattt cacccagggc tacaagaact acgtgtgctg cagagacgag
      961 cacagggtca tgttctgccg cagcgacatg accgacgtcc acctcttcga cgccgtaaac
     1021 gatgtggacc agagaaggga ccttgccaaa gccgagcccg agacggtgaa gaggatgtac
     1081 gaggattacg cacagaaaga cgcagcggga cgccttccga acttttgaat tggcctaagc
     1141 tacaggcttc agtgctcaga cttcagccgc ccgcagccat ggctagaccc atgccaccag
     1201 attcgaacgg tcgcaatcct ggcttccaac agaggaaaaa acagccgcag cgacttgacc
     1261 tggaaacgat gctggtctac agtcttccga tgtccataat tttaatagac aaagggatta
     1321 atcgtcctcg cggtatgctc taatgacgtt acggcgtatc ccggcatcat gcttggaagg
     1381 accggcagag tccggacgct aaccctcatg agtacgagag agaggcgcat gtgacacaga
     1441 gcaaacctgt tgcgttgtgg gctgtaccac gttcgatatc tacggcgttc gagcgggtct
     1501 ttgtggagag ggacgacttc gaggtactgc acgagccgtt ttcggcctct tactactatg
     1561 gggaggatcg cctgagcgac cgctttgtgg atgccgagcc gaaggccgag tacagttacg
     1621 agcgggtgct cgaggacgtc ttcaggccgc gcgagcagcg cgtcttcgtc aaggatatgg
     1681 cctaccacgc aaagaaggtg ttgagcaccg agttcgcctc gcggttcgtc aatacgttta
     1741 tcgtgaggga cccgaagtac gtattggtgt cgctgtacaa gatgtggccc gactttacgc
     1801 tggaggagac aggctacgag gatttgtaca ggctcttccg ttatgcgacg gacgcggggg
     1861 aggacgttgc cgtcgtcgac gcgatgacct tctcagagaa cccggctggt gtgctggccg
     1921 cctactgcga gcatctcgag gtgcctttca ggtcaggctc cctcacctgg gagtcggggg
     1981 aagtcagcga gtgggacagc tgggagggat ggcatgagga tgccgagagc agcacaggca
     2041 tagagccggc cgagcgcctg gaccccgagt tgcccgagga gctcgaggcc gtctacgagc
     2101 agtgtttgcc ctactactac gagctggcgg cgcatgccat cccatccgtg gcgcggcggt
     2161 ccgtcccggg acgtacggac gacaaggaga agatcttgct gagaacagag agtgcgatag
     2221 ccccgcacgg cggagaactc atagaccgcg tggtccccga ggcggagcgc agggagcggt
     2281 cgatggaggc cgcggagcta ccgaaggtgc cgttgagccc gcgggcccta tccgacctgc
     2341 agatgatctc gaccggggtc ttcagccccc tcgaagggtt catgctgcgc gaagagtacg
     2401 agggcgtggt ggaggacatg cgcctgaaag acggcctcgc ctggagcctg cctgtcaccc
     2461 tatccgttga cgaggagaag gccagagcgc tgtcggataa ctctgagata gccctggtcg
     2521 acggtaccgg agaacccgtg gccaccatgt tgctcaggga gctctacggc tacgacaagg
     2581 agcgcgaggc cagtatggta tacaggacga ccgacgccga ccatcccggc gtcgccgcgg
     2641 tctacaggca gggcgacgtc ctgctcggtg gcgaggtaga gctcctgcgc ccgccggacg
     2701 aggggcgttt cccccgctac tactacacgc catcgcagct cagggccacc ttcgccgaga
     2761 aggggtggaa gcggatcgtc ggcttccaga ccaggaaccc cgtacatcgg gcacacgagt
     2821 acatccagaa gagcgccctg gagacggtgg acgggctcct cttgaacccg ctcgtcgggg
     2881 agaccaaatc tgacgacata ccggccgacg tacggatgag atcctacgag acgatcctgg
     2941 acaggtacta tcccgaagac cgcacgatgc tcacggtctt ccctgcagcg atgcgctacg
     3001 ccggtccgag ggaggccatc ttccacgcca tttgccgcaa gaactacggt tgctcgcact
     3061 tcatcgtagg ccgcgaccac gccggcgtcg gcagctacta cggcacctac gacgcccagc
     3121 acatcttcga ggagttcgcc ccggaggagt tggggatcac acccctcttc ttcgaacacg
     3181 cgttcttctg cacggagtgt gggggtatgg gctcatcgaa gacctgccca cacgacccag
     3241 actcccacgt cttcctctcg gggacgaaag tccgcgagat gctctccagg ggtgagtacc
     3301 cgcccccaga gttctcccgc cccgaagtga tcgaggtgct tatggatggt cagcgggtcg
     3361 agagcaggta gcgatcagct ctcagcggtc acctctttca cgccagacgt aaaccggcgc
     3421 agtgactcgc gacgctgact accactacta tggcgagcga gatgagcata tagatgcatc
     3481 cggcgtcgag gacgttcctg agtcccacaa cggccggtag gaccagccag gcggccacga
     3541 gggtcgagaa gaaaaccgcc tcggctccca ccatcgcagc cgccacctca cacaagagca
     3601 gcgaagccaa cacgaccgac ccgagcgtgg gcccgagccg tccgcgggcc gcgctaccct
     3661 taccgtgtac ccggccattc gctcctgatc tgtacaagaa accggtcaag tcccctctcg
     3721 cccgtcctcg atgatttcag atccggcatc cggcgcgtgt ccggtgtcgg gaccatcacg
     3781 cgcctcaccc cgcaggtcgt accaggtatc caacccctga ctgccagccc tgtcccaaag
     3841 ctcgtaccag agatccaggt cccacttcat cgtcccgcct ccactctact tgtgtttccc
     3901 tactttataa gtagacatta tggtatacga ttgctctcac ggcgtcaagc gccggggctt
     3961 cacttggggc ctgatcgcgg gcaacagacg cgatcgtctc agacatccag gagtgttgat
     4021 ccggtgagga tttcatgtat tctttaccat tgcaatagac aaagtagttt tagtagacat
     4081 taagctggta atcgggagaa ggtttatggg tactagatca gggctcgccg atcctgtgct
     4141 gtcgtatttg atctgcgcga cgccgcgtag cgggagcacg ttgctttgtg cggcgctcga
     4201 cgacacgggg atagcgggcc accctgagga gcacttcgag gtcttgctcg agacggggca
     4261 acccaggcaa ccacgagact acttccagcg ctccaacgat cctgaggtgt gggcgctcct
     4321 cgacgatcct gagttcaggg acgttctggg cgagtacggt ggcaggtact ccgagcatcc
     4381 ggcgcgcagg gacccgcttt ggcgcccgcc cgacttcgag aggctggcag aaggggcgct
     4441 gcacaagggc acgaccgaaa acaacgtgct tgggaccaag atcatgtggg catatttcag
     4501 ggacttcgtc cgactcgcac ggcggacgac tgcgcccgac gtccggccct gcgaggtacc
     4561 cgcctccgtc ttgcccaacc tgcggcggtt cgtctggatg cgtcgcaggg acaccacccg
     4621 ccaggccgtc tcgctgtgga aggcgctcca gactcagcat tggcggcgag actcggatga
     4681 ggatgtaggt ggacagggcc tgcgtttcag ctttgccgcc atagaccact tgaagctgcg
     4741 tatcgacga
//
LOCUS       DASTKM010000163         3994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_748381, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000163 DASTKM010000000
VERSION     DASTKM010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748381"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VFQ10_06770"
     CDS             <1..1281
                     /locus_tag="VFQ10_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HET9965080.1"
                     /translation="RALKERVVRHLDEYLLQLEESVTRAGGEVHWARDAEEANQIIAG
                     IAKEKGAEEVVKVKSIATDETKLNEHLEREGIRAFETDLAELIIQLAGETSSHILVPA
                     IHKNRAEIRELFIEKLGAKNLSDRPSDLTEAARLYLREKFLAAKVGISGANFAVADTG
                     TVCVVESEGNGRMCTTLPPVLVTLMGIEKVIPAWRDFEVFMQLLPRSSTAERMNPYTS
                     FWTGVSGEAGDGPREFHLVLMDNGRTDVLSDEIGRQALNCIRCSACLNVCPVYERTGG
                     HAYNSVYPGPIGAILTPQLVGIGDPGPDSLPYASSLCGACYQVCPVKINIPEVLVHLR
                     GRVVRHRQYMGGPRSKLDPENVAMQTLARVFSDPQLYERAQRMARVGQWPFARGGSIG
                     RLPGRLAGWTAVRDLKPIPQQTFREWWKRNRGAF"
     gene            1281..1937
                     /locus_tag="VFQ10_06775"
     CDS             1281..1937
                     /locus_tag="VFQ10_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET9965081.1"
                     /translation="MATAKETILWRIRHATRDVPEAERHEDVSVEREYRKEEGAEHEE
                     IVALFAERTAEYEADVRRVGHDELPGAIEEAMKRRGVRRLVIPPYLPREWIPDGIEIM
                     RDAARSRLTNQELDESDGVLTGCALGIAQTGTIVLDAGRAQGRRALTLLPDYHLCVVR
                     EQQIVGLVPEAFASLERAVKGEGRAITFISGPSATSDIELDRVEGVHGPRTLEVLIVG
                     "
     gene            2026..2259
                     /locus_tag="VFQ10_06780"
     CDS             2026..2259
                     /locus_tag="VFQ10_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965082.1"
                     /translation="MRDLYEYRRRRFVARHSEQTEGDDYEECSLAYQRFGRELLGAER
                     AAFLPLRSEGRISDEVRRRVERDLDLEDARLEI"
     gene            2368..3852
                     /gene="gltX"
                     /locus_tag="VFQ10_06785"
     CDS             2368..3852
                     /gene="gltX"
                     /locus_tag="VFQ10_06785"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET9965083.1"
                     /translation="MENHAKIRVRYAPSPTGRLHVGGVRTALFNWLFARKNGGTFVLR
                     IEDTDLERSTEESVEQLGRSMRWIGLDWDEGPGAGGPHPPYRQTERFDLYREAAKKLL
                     DSGAAYYDFATPEELAGLRERARAEGRQPIYTGGEYREMDPDETRERIESGEPYTVRF
                     KTPREGQTVVQDMIRGPVTFENANLEDFVLMKSSDTPTYNFAAAFDDAEMEISHVIRG
                     DDHLSNTPRQILIHEALGNPLPAFAHVPQVLGPDKKKLSKRHGAASVEDFAERGYLPE
                     ALFNYLALLGAGYAADQEIFTPEELAERFRIDRVSGNPAILDEQKLRSVNAVYLRRKS
                     SEELATLAAPMLVESGAATQEELERDPARLEDIMHLLKERIALTTEIPDSVGYFYGGS
                     LDYDETEFEKQFGKEFVRETFPVLVERLTALPEWTETAIEQAVRGLAAEREKGARHLI
                     HPLRFAVTGRTVSAGLFETMQLLGRDRSLLRAGDAARKMQRLSV"
     gene            3921..3993
                     /locus_tag="VFQ10_06790"
     tRNA            3921..3993
                     /locus_tag="VFQ10_06790"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3955..3957,aa:Glu,seq:ctc)
BASE COUNT          806 a         1304 c         1285 g          599 t
ORIGIN      
        1 cgcgccctca aggaacgcgt cgtgcgccac ctcgacgagt acctcctgca actcgaagag
       61 tccgtgacgc gcgccggagg cgaggtccac tgggcgcgcg acgccgagga ggcgaaccag
      121 ataatcgcag ggatagccaa agagaagggc gcagaagagg tcgtcaaggt caagtccata
      181 gcgacggacg agacaaagct caacgagcat ctggagaggg aaggtatcag ggccttcgag
      241 acggatctcg ccgagctcat catccagctc gcaggggaga cgagctcgca catcctggtg
      301 ccggcgatcc acaagaaccg ggcggagatc agggagctct ttattgagaa gcttggcgca
      361 aagaatctct ccgacagacc ttccgatctg acagaggcag cccgtctgta tctgcgcgag
      421 aagttccttg ctgcgaaggt cggcatctcg ggggcgaact tcgctgtggc tgatacgggg
      481 accgtatgcg tcgtcgagtc cgagggcaac ggcaggatgt gcacaacgtt gccgccggtc
      541 ctcgttacgc tcatggggat agagaaagtc atcccggcct ggcgggactt cgaagtattt
      601 atgcagctcc tcccccgctc ctcgacagcg gagcggatga acccttacac ctcgttctgg
      661 accggggtct cgggcgaagc cggtgacggg ccgcgggagt tccacctggt cctcatggac
      721 aacgggcgta ccgacgtgct gtctgacgag ataggacgcc aggccctgaa ctgcatccgc
      781 tgttcggcgt gcctcaacgt ctgtcccgtc tacgagcgga ccggaggcca cgcctacaac
      841 tccgtctacc ctggccctat aggcgcgatc ctgacacccc agctcgtcgg gatcggcgac
      901 cctggcccag actcgctgcc ctacgcctcg tcgctttgcg gggcttgcta tcaggtttgt
      961 ccggtcaaga tcaacatccc agaggtcctc gtccacctcc gcggcagggt cgtccgccac
     1021 aggcagtaca tgggcggccc ccgctcgaag ctagaccctg agaacgtcgc gatgcagact
     1081 ctcgccagag tcttctccga cccacagctc tacgaaaggg cccagaggat ggcgagggta
     1141 ggccagtggc ccttcgcccg cggaggctct atcggacgcc ttccaggcag gctcgccggt
     1201 tggaccgccg tacgtgacct caagcccatt ccacagcaga ccttccggga gtggtggaag
     1261 aggaacaggg gggccttcta gtggcgaccg cgaaagagac gatcctgtgg cgtataagac
     1321 acgccacccg cgacgtgcca gaggcggagc gtcacgaaga cgtatccgtc gaacgcgaat
     1381 accgcaagga agagggcgcg gagcacgagg agatcgtcgc gctcttcgcc gagcgcacgg
     1441 cagagtacga ggcagacgtc cgtcgcgtgg gccacgacga gctccccggg gcaatagaag
     1501 aagccatgaa gcggcgcggc gtgagacgcc tcgtcatccc cccttacctc ccgagagagt
     1561 ggatcccgga tggcatagag atcatgcgcg acgcggcccg ttcccggctc acgaaccagg
     1621 agctcgacga gagcgacggc gtgctgacgg gctgcgccct cggcatagcc cagacgggca
     1681 ccatcgtgct cgatgccggc agggcccagg gccgccgggc gctcacgcta ctccccgact
     1741 accacctgtg cgtcgtgcgc gagcagcaga tagtcggcct cgtaccagag gcctttgcca
     1801 gcctggaaag ggcggtcaag ggcgaaggca gggccatcac ctttatctca ggtccgtcgg
     1861 cgacctcgga catcgagctc gacagggtcg agggcgtgca cggcccgcgt acgctcgaag
     1921 tgctgatagt cggctgagat agccggcgac cgcaaggggt cacacggatt tcaggattgg
     1981 ctgccaaccg gccggaggag cgaagataga aagaacttct cccggatgcg cgatctctac
     2041 gagtaccgtc gccggcgctt cgtcgcccgc catagcgagc aaaccgaggg cgacgactac
     2101 gaggaatgct ccctggcata ccagcgtttc gggcgggaac tgctgggtgc cgagcgtgcc
     2161 gcgttcctcc cgctcaggag cgaaggccgc atcagcgacg aggtgagacg acgcgtcgaa
     2221 cgggacctcg acctcgagga tgcgcggctg gagatctagc cgcggcccgg atgaccccgg
     2281 cgttctgcgc cagaaccact tgagggaagc attaccgggg agcagaacgg ccgcacggcc
     2341 cagaccaacc tgtgttaggt ttagattatg gagaaccacg caaagatcag ggtcaggtac
     2401 gcgccgagcc cgaccggcag gctgcacgtc ggtggtgtac gcacggcgct cttcaactgg
     2461 ctcttcgcgc gcaagaacgg cggcacgttc gtgctgcgga tagaggacac ggatctggag
     2521 cgctccacgg aggagtcggt cgagcagctc gggcgctcga tgcgttggat cggcctcgac
     2581 tgggacgagg gcccaggggc cggcggccca catcccccat accgccagac cgagcgcttc
     2641 gacctctacc gcgaggccgc aaagaagctc ctcgactcgg gcgcagccta ctacgacttc
     2701 gcaacgcctg aggagctcgc cgggcttcgc gaaagggcgc gggccgaagg acggcagcct
     2761 atctacaccg gcggcgagta ccgtgagatg gaccccgatg aaacgagaga gaggatagag
     2821 tcgggcgagc catacaccgt ccgcttcaag acccctcgcg agggccagac agtggtgcag
     2881 gacatgatcc ggggtcccgt caccttcgag aacgccaacc tggaggactt cgtgctgatg
     2941 aagtcctccg acaccccgac ctacaacttc gccgccgcct tcgacgacgc cgagatggag
     3001 ataagccacg tcatccgagg cgacgaccac ctctcgaaca cgccgcgcca gatcctgatc
     3061 cacgaggccc tcggtaaccc gctccctgcc ttcgcccacg tcccccaggt cctcggacca
     3121 gacaagaaga aactaagcaa gcgccacggc gcggcgagcg tcgaggactt cgcggagcgg
     3181 ggctacctgc ccgaagcgct cttcaactac ctcgcgctcc tcggggccgg ctacgccgcg
     3241 gaccaggaga tcttcacccc ggaagagctt gccgagcgct tccgcatcga ccgcgtgagc
     3301 ggcaacccgg cgatcctcga cgagcagaag ctaaggtctg tgaacgccgt gtacctgagg
     3361 cgcaagagct cagaagaact cgccactctc gccgccccca tgctcgtcga gtccggcgcg
     3421 gcgacgcagg aggagctcga acgggacccg gcccgcctcg aggacatcat gcatctcctg
     3481 aaagagcgca tcgcgctgac gaccgagatc cccgactctg tgggttactt ctacggcggc
     3541 tcgctcgact acgacgagac cgagttcgag aagcagttcg gcaaggagtt cgtacgcgag
     3601 accttccccg tgctcgtcga acgcctcacg gcactccccg agtggacgga aacggcgata
     3661 gagcaagcag tgcgaggcct ggccgccgaa agggagaagg gcgctcgcca cctgatacac
     3721 cccttgcgat tcgccgtcac tggacgcacc gtgagtgccg gactgttcga gaccatgcag
     3781 ctcctcgggc gcgaccgcag cttgctccgc gcgggagacg ctgcccgcaa gatgcagagg
     3841 ctgtccgtct agagggcgac ctcgaaccct tttgacgaag cggagaccgt cgggtagaat
     3901 cttcccctgc ccgtcggcaa ggccccatcg tctagaggcc aaggacatcg ccctctcacg
     3961 gcgaaaaccc gggttcgaat cccggtgggg tcac
//
LOCUS       DASTKM010000164         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1101191, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000164 DASTKM010000000
VERSION     DASTKM010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101191"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFQ10_06795"
     CDS             complement(<1..574)
                     /locus_tag="VFQ10_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9965084.1"
                     /translation="MAEAGAEGREQRGQQGHLQQGLKRRHMTMISLGGIIGAGLFVGT
                     GPILNQAGPATILTYLLTGCILILIMRMLGEMAVAQPSVGSFSDYSRMALGNWAGFSI
                     GWLYWYFWAIVVGFEATVAASIIGQYISGIHPALISLILVLALTATNLYSVGSYGEFE
                     FWFAGIKVAAIIIFIVLGALFVIGLWPGGGG"
     gene            complement(659..1810)
                     /locus_tag="VFQ10_06800"
     CDS             complement(659..1810)
                     /locus_tag="VFQ10_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9965085.1"
                     /translation="MIHFSREELAARRRAAAGELASRGLDGLLMFRQESMYYLTGYDT
                     FGYVFFQCLYMGDDGETFTLLTRTPDVRQAGHTSVVEDIRVWYDAEGADPAGDLREIL
                     EKHGCRGKRLGIETDAYGLTGHNLKRVETALDGFCELVEASDLVSRLRVIKSPAEIAY
                     VREAARLADLGLDRAVEVAAPGVFEGDVLAALQGAIFEGGGDFPANEVIIGSGPRALL
                     FRSAAGMRRMDPVDQLSLEWAGSYRHYHAAMMRTLAVGEATGYQRYLFSAVREALEEM
                     TEALAPGRPVGDVDDAHRRVLDAAGLEEHRFAACGYSLGTTFSPNWMDWPMLFSGNPV
                     LAEPGMVFFLHCVVADSGRGVAMSLGRTCLVTPSGREVLSARPLNLIVV"
     gene            complement(1835..>2386)
                     /locus_tag="VFQ10_06805"
     CDS             complement(1835..>2386)
                     /locus_tag="VFQ10_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET9965086.1"
                     /translation="GMRALVSVLDINPKRLAYLSDIFEGRADLVIPNRARTAAYVRQA
                     DVVIGAVLVHGAKAPKLVTREMVASMRPGSVVADVAIDQGGCIETARPTTHSDPTYVE
                     EGVVHYCVANIPGAVARTSTLALTSATLPYLIRIADKGIEGAAAEDEALAKGLSTLGG
                     DLVSEPVAEAHDLSYTDAGKILG"
BASE COUNT          438 a          820 c          710 g          418 t
ORIGIN      
        1 ccccacctcc ccctggccac aacccgatca cgaacagcgc cccgaggacg ataaagatga
       61 tgatcgcagc tacctttatc cctgcgaacc agaactcgaa ctcgccgtag gagccgacgg
      121 agtagaggtt ggtggcggtg agcgccagta ccagaataag ggatatcagc gcggggtgta
      181 tccccgatat gtactgtccg atgatgctcg ccgcaacggt ggcctcgaac ccgaccacga
      241 tggcccagaa gtaccagtag agccacccta tggagaatcc ggcccagttg cccagagcca
      301 tccggctgta gtccgagaag gaaccgaccg acggctgcgc cactgccatc tcgccgagca
      361 tccgcatgat caggatcagg atgcaccctg tcaagaggta ggtcaggatg gtcgctggac
      421 ctgcctggtt gaggatcggg cccgtcccca cgaacagccc agccccgatg atcccgccca
      481 gggagatcat ggtcatgtgc cgcctcttca gaccctgctg cagatggccc tgctgccctc
      541 tctgctctcg cccttccgca cccgcttcgg ccatgttgtt tccccttccc taaggtcgtt
      601 gccttgcgcg cagcttacag gctaaacacc atttcttcca acgtatcgga agtctggatc
      661 agacgacgat cagattcaga ggccgtgcgg agagaacctc tctccctgaa ggggtcacca
      721 ggcaggtgcg gcccaggctc atggcgaccc cgcgccctga atcggccacc acgcagtgca
      781 agaagaagac catccccggc tcggccagca cggggttccc tgagaacagc atcggccagt
      841 ccatccagtt ggggctgaaa gtcgtgccca gagaataccc gcacgcggcg aaacgatgct
      901 cttccagccc tgcggcgtcc agtacgcgcc gatgggcatc gtcgacatcg ccgaccggac
      961 gtcctggagc cagggcctcg gtcatctcct cgagcgcctc gcgaacggcg gagaagagat
     1021 atcgctggta gcctgtagcc tcgcccacgg ccagtgtacg catcatggcc gcgtgatagt
     1081 gcctgtagga gcctgcccac tccagagaca actggtcgac agggtccatg cggcgcatgc
     1141 cggcggctga gcggaacagg agggcgcgtg gcccggagcc gatgatcacc tcgttcgcag
     1201 ggaagtcccc gcctccctcg aagatcgcgc cctgcaaggc cgccaggaca tccccctcga
     1261 agacgccagg cgccgccacc tccacggccc tgtccaggcc gagatccgcg agccgcgcgg
     1321 cttcccgcac gtaggcgatc tcggcggggg acttgatcac ccgcaaccta ctcacgagat
     1381 cggaggcctc caccagttcg caaaacccat cgagcgctgt ctccacgcgc ttcaggttgt
     1441 ggcccgtgag gccgtaggcg tcggtctcta ttcccagtcg ctttcccctg cagccgtgct
     1501 tttctaggat ctccctcagg tccccggcgg gatccgcgcc ttctgcgtca taccagaccc
     1561 tgatatcctc gaccaccgac gtatgcccgg cctggcgcac gtccggggtg cgggtcagga
     1621 gggtgaaggt ctcgccatcg tcgcccatgt agaggcactg gaagaagacg tagccgaacg
     1681 tgtcataccc cgtcaggtag tacatggact cctggcggaa catgagcagc ccgtcgaggc
     1741 cgcggctcgc caactctcct gcggcggccc tacgacgggc tgccagctct tcgcgagaga
     1801 aatggatcaa gtccagccgc gctggtagac tcccctaccc aaggatcttc cctgcgtcgg
     1861 tgtacgagag gtcgtgggcc tcggctaccg gctcggagac gagatctccc cctagcgtag
     1921 agagtccttt ggccagggcc tcatcttcgg cagccgcacc ctcgatacct ttgtccgcta
     1981 tcctgatcaa atatggcaac gttgcgctgg tcagggccag cgtcgaggtc ctcgccacag
     2041 cccccggtat gttggcgacg cagtagtgca cgacgccctc ctcgacgtac gtgggatctg
     2101 agtgcgtcgt cggcctcgcc gtctctatac acccaccctg gtcgatggcg acgtcggcga
     2161 ccacgctccc tgggcgcatg ctcgcgacca tctcacgcgt gacgagcttg ggcgctttcg
     2221 ccccgtgcac cagcacggcc cctatgacca cgtcggcctg cctgacgtag gcggcggtcc
     2281 tggcgcggtt gggtatgacg aggtcggccc gtccctcgaa gatgtcagag aggtaggcga
     2341 gacgcttggg gttgatgtcg agcacgctca ccagtgccct catgcc
//
LOCUS       DASTKM010000165        21807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_220121, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000165 DASTKM010000000
VERSION     DASTKM010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 21807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21807
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220121"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFQ10_06810"
     CDS             <1..665
                     /locus_tag="VFQ10_06810"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HET9965087.1"
                     /translation="NVPPLPALPEPLRGKSVIALRGCYCGESPGDGEELVRPMREELG
                     EPIMDAFGLMPYAAMDSISMDPVDPMGARQHSEMLSELSPEAIQTLVEVAGAGSGSPL
                     IMLELRQLGGALARTAEHLSTMGKGDSKFIMNGIGPAFTPEMADGVVTYLARVADATR
                     SFQTGDTYVNFMELEGASAERVKAAYAPEDFERLVALKDRYDPHNVFRFNRNIAPSTA
                     EH"
     gene            768..2108
                     /locus_tag="VFQ10_06815"
     CDS             768..2108
                     /locus_tag="VFQ10_06815"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET9965088.1"
                     /translation="MQAEREAQEKVARPLPEDTLWVRFVFVDHAGIPKAKAVHRDGFD
                     GRARAGVGLAKGVLALDPSGALHPESGLSPVGECRLVPDLSTLTALPFARGQAMVSVD
                     MTEPDASTPWEGCPRSALKRVLERLSDRGYRTLASFEAEFYVWDAEGPLDRTPYAGSY
                     ALTPAAEFVAELAQTLEEMGIRPEQCHAEVGQGNLELSVGEDEALAAADKRVMVLEAI
                     RGVAHRMGLGTTMAPKPYLEGAGNGHHLHVSLYEDNAPVLFNPSGTLSGRGSGFVAGI
                     LEHLPAVMAFSAPSPNSYQRLAPGMWASAYAAYGLDNREAAVRLASPVAGRESATANV
                     ELKPVDVTSNPYLALAAVLAAGMDGMDRGLDPGEPTMVDPATLSDEERASRGIPPLPA
                     SPDEALDALEGDHALKEALGEPLVRTHVAVARAQAKIARELPPEEVASTAAMLY"
     gene            2264..3403
                     /locus_tag="VFQ10_06820"
     CDS             2264..3403
                     /locus_tag="VFQ10_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9965089.1"
                     /translation="MEALDLTRIPVVDNHCHGLLRDQAFEDIASWRQAFTESTDPGMP
                     RDHVASTALYRRLIRMLAYFLGCEPEEEAVYSARTEKDGRELTGALLRAANVDTFLLD
                     TGFPPPETVLQVAELGELGGCRAEPMLRLEILMESLLAEKDSLEDVKEALAAELDDVR
                     SRSYVALKSIAAYRTGLDIREWPREEAEESFHEYRRVTGAGPARLVHKPLLDTLLHVA
                     FAQAARQEVPVQFHVGYGDADTDLLLGNPLYLRPVLQRPDYRGLPVVLLHECYPYTRQ
                     GGYLAAVYENVYLDLSYGIPPLGYGEMVSFTRQALGVAPTSKLVYSSDGIGVPELHWI
                     SAIDGRRAIGQALGEMVAHGELSLPEAEAAGESILRDNATRLYRL"
     gene            3570..4043
                     /locus_tag="VFQ10_06825"
     CDS             3570..4043
                     /locus_tag="VFQ10_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965090.1"
                     /translation="MYRQTRLLGVLLILQVGAMVGIGVWLFSRIDWDRVIELTNKQGQ
                     IQVKSIDGALLKPLEQAILYFIYFLPPAILLLLAGLGFVLLRRRGWMLASVAQALIMM
                     ACLFFYTDPRPWFAYPIIAYCILMILYLNSQGVRTVVHSRRMTPAGGRSETAHGE"
     gene            4033..5895
                     /locus_tag="VFQ10_06830"
     CDS             4033..5895
                     /locus_tag="VFQ10_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965091.1"
                     /translation="MANSASSEVRDAETLLRRFEPVIRFTRGEWFYPMDAESYVDACS
                     LWVRHPGEDAVCVVPAGDLTLERIAQQPEDSSGAVHYLKIPTSKEDERRGLLARLRAR
                     RSYKNPDWPREKFRAGRGRLARVGYISRLADALFSLVLLARGRVPGGDVEGARVLYGR
                     LMEKEERFRYHGRVIRQDGWIVLQYWLFYAFNDWRSNFFGANDHEADWEKVFVYLSES
                     GEVRPEWVAYAAHEETGDDLRRRWDDPELEKVGEHPIVYVCAGSHASRYSAGEYLTEL
                     AFPLPRAFAATRRVARRFWYITLRQYAEEYSAEKPYERFTIPFVDYGRGDGLSVGPGQ
                     DMEWDPPRLMIDPLPSWVAGYRGLWGLYTRDPFEGEDAPAGPMYDRDKTISREWYDPV
                     GWAGLDKVPTRTEELDVIIKRQAEIEERRARLRSEIQEMSSRLKALGMDAEATRGRSH
                     LSKLYETQTRHIEVLSKEVEEMRAELSEDEVLHESLSDHAEHLRNGERSPVHAHLADP
                     VRPVSEAETHAGRVAQLWASISVAVVLLALIVIFAFQRQHLLSALVFAIAMFAFIEAG
                     FRRRLTRFIVSANIGLAIVGALVLLQHYFWQIIVLAVLAVGLYILWENLRELGR"
     gene            complement(5961..6230)
                     /locus_tag="VFQ10_06835"
     CDS             complement(5961..6230)
                     /locus_tag="VFQ10_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9965092.1"
                     /translation="MARSHRVTFQRSGITIDVAEDEYILEEAEDAGLHLPYDCRSGTC
                     TTCIQKCLEGEIDQDMAFAIGDEELEQGLRLICIGSPLSDVVLDA"
     gene            complement(6347..6445)
                     /locus_tag="VFQ10_06840"
     CDS             complement(6347..6445)
                     /locus_tag="VFQ10_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET9965093.1"
                     /translation="MLIRFDGYRGRYVFHCHNLEHEDMMVGNFEVV"
     gene            complement(6566..8872)
                     /locus_tag="VFQ10_06845"
     CDS             complement(6566..8872)
                     /locus_tag="VFQ10_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9965094.1"
                     /translation="MYKEIYVPVDNSDYSNQACVIGVDVARQFGGRVAGCHAYAAKMH
                     DVRFRQMESGLPEEFRDEDEMKRQRKIHDQLITKGLEIITDSYIDVLEPLCEKYDVEL
                     VRRSLEGKNFKVIVEDVNQNDFDLVVMGAMGMAAVKDTVLGTVTERVVRRLEKADTLI
                     VKDLDRSPFEHIVVAVDGSAKSLGGLKRAIELAREFGGTVEAISVFDPYFHYAMFHSI
                     AGVLSTKAQKVFRFKEQEKLHEEIIDSGLAKIYTAHLEIARKIAEDEGVDLKTTLLAG
                     KPFEQTLKYVNEVNPTMVVMGRIGYHSDDEMDIGGNTENMMRYLPTNVLITNYEYQAP
                     DLYTAEEHMTWTKEALERLDRIPGFVVGMATGAIIRYAIEKGYTVITTGVIDEVIKSI
                     LPPGAMESMRAIGDQMRAAEAMGEDKPIDSLFKDFDERTADKANGHNNGANGSDSAAK
                     QEIFEAGRGGFGKSEDQGDIEGVSAEVQDEIRRAAQGQDRFECDVCRYVAKGKPAKCP
                     VCGSGPTHFHAVDAHIATTVEGENLNLSEVYDGRELHWTDDATALLDSLEEWQEKRRV
                     KARVEKSALKKGYTTITREYVEQQYREETGREAPEVKSGGCPVSHAKQQVQETSGAKC
                     PIDHSAFQKAGASVPEGGSREFVWTQDAIDRLERAPKGFMRNISRNMTEKLARERGTT
                     HIDLALVEDSLTGARTTMEDVITGKVSIADLAKDTGEKIATENGEAPHPPMTVTMVCT
                     TCGEELEGVQPPEECPVCGAPGEDFEQR"
     gene            9097..10038
                     /locus_tag="VFQ10_06850"
     CDS             9097..10038
                     /locus_tag="VFQ10_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET9965095.1"
                     /translation="MDLEKMEQDARAAKDSRSYTDGAAFVDGRFLPVGEARVPILDWG
                     FLRSDATYDVAHVWRGSFFRLEDHLDRFERSMEHLHLGPPYDRSEIRDILIECVRLSG
                     LRDAYAEIICTRGIPRPGSRDPRDCENRFYAFVVPFIWIADPEKQERGLHAVIGSKHR
                     IAPESVDPTVKNYHWLDLETGLLEAYERGGETVILVDAEDNVVEGPGFNVFAVEHGTI
                     STPDRGVLEGITRKTIIELAAEHGMQPEARPIPAGELGQADEVFLSSTAGGVIPVTTV
                     DGEAVGNGVPGPVTRQLRDAYWDLHDDPRFSLPVHYD"
     gene            complement(10190..12907)
                     /locus_tag="VFQ10_06855"
     CDS             complement(10190..12907)
                     /locus_tag="VFQ10_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9965096.1"
                     /translation="MNEQENGYPGLILPFTAIDRGALAVAGGKAANLGDLVRAGFPVP
                     AGFCVTTAAYDLVAADAGLEPTLAALAGTQPEDTARLAGLAKQARSYLSDAIVPDLLR
                     QAIAEAYEGLGDGAPVPVAVRSSATAEDLPQASFAGQQDTYLNVIGVESVLDAVRRCW
                     ASLWTDRAVSYRATNGIDPHSVRLAVAVQRMVDASVAGVLFTANPVTGKRRQAVIDAS
                     PGLGEAVVSGMVNPDHFVANTATVEIVERRLGDKRVAIMAGPGSGTERVVLDAGEDSA
                     SLSDGQVLALAKLGERVEEHYGMPQDTEWAIDDDGGIWLLQARPITTLFPLPERATRS
                     EEDLRVYFSFSVAQGVYRPLTPMGIQGFRLITSALAMLAGRAPSNPYEGAAFFTESAG
                     RIFADITPVLRSKQGRALFRGVMRNMEARTVPIVRHLAADPRLSPVPTPIRPVLHAVL
                     SVFVRGRIPLRFVEALARPEKARARAARVEARSRAAGHVSPKASAAERLSAAERLLLG
                     GPPDMLPNVPPVFAAGLAANVLAGKLLGGLATDDERRVAMRALPHNPTTEMDLALWAL
                     AKEARADPDIARMLGETPPERLAEDYRRGSLPPKLQTSLADFLRLYGHRGVAEIDLGL
                     PRWSEDPTYILGVLANYLRLDDPQAAPDVQFGRATREAEEMVGELTRRAAREGRLRGA
                     LVGFLLGRARALSGLREMPKFCIVLLMARARELLWTVGVELAEAGHLQSAGDIFFLSV
                     PEAWAALAGEDLRPLVRDRRAVYEQELGRRHVPRLLLSDGTEPTAGTHHATGADGVLR
                     GTPASGGVVTQKARVILDPGEASLEPGEILVAPSTDPGWTPLFLTAGGLVMEMGGPMS
                     HGAIVAREYGIPAVVGVPDATGRISTGQRITVDGSAGEVTPDLEGAGPA"
     gene            complement(12986..13723)
                     /locus_tag="VFQ10_06860"
     CDS             complement(12986..13723)
                     /locus_tag="VFQ10_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9965097.1"
                     /translation="MRRTLLVAGRVVRQLFRNPRFLVLSVAAPLVIVYLLKIFFDTLP
                     ETFDVARYAVPVAAFLVHFLAFLLCAISLVQERTMGTMERMFVNGFRRMEIIAGYVLG
                     FLGLATFQAVAGLAEAIWLFELDYDEETLAMLFVVVWLLAIASVMLGIFISTFARHEG
                     QVFPFVPLIIVPSVFLSGLLVDVDELPNWADWLGHAFPLFWANDVIQDVIESGWTFED
                     VQGSLAVLVAYSVALLLLASLTLREVE"
     gene            complement(13723..14574)
                     /locus_tag="VFQ10_06865"
     CDS             complement(13723..14574)
                     /locus_tag="VFQ10_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9965098.1"
                     /translation="MNAIEVSGLTKMYGRVEALGGTDLSVRRGEVFGLVGPNGAGKTT
                     LIKGLVGALRPSGGIARVMRLNPLEERAELRQRIGYMPQGAALYEDLSARDNVWFFGA
                     AHRTPDLKKKVDEVLHFTDLSGRAYDPVHTFSGGMKRRVSLACALVHGPEVIFLDEPT
                     AAVDPQLRSRLWKTFRELAGGGTTLFISTHLMDEAMLCDRIAILLKGRIIATDTPRSI
                     LERGRTRLTVKQDGREEEETIGGHPEDLAAALRPYGLRPDVKAVDVKTDSLEAVVLSL
                     IEKQEVG"
     gene            complement(14571..15185)
                     /locus_tag="VFQ10_06870"
     CDS             complement(14571..15185)
                     /locus_tag="VFQ10_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9965099.1"
                     /translation="MTEESDRRAQILEAAFEEFSAKGFKGATIKSIARTASLQSPALI
                     YWYFPDKEALFREVLGSRIPVLRAVRDPARLLDLPPEEVLPNIARRYLSTFDDRATQR
                     MARLLVGEAMRRPEVSEVFGNAVIKRVLGFLKSYMARQVELGRLRPHDVRASARAFIG
                     MLLPQAGGKMFFAAIREDGLTDEEHIEAVAGIFLRGLEGKEDKG"
     gene            15316..15444
                     /locus_tag="VFQ10_06875"
     CDS             15316..15444
                     /locus_tag="VFQ10_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965100.1"
                     /translation="MIYILIQLINGALTAVLIGWVLLQIVGLWAMIDAYTTAKRNN"
     gene            15473..15664
                     /locus_tag="VFQ10_06880"
     CDS             15473..15664
                     /locus_tag="VFQ10_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965101.1"
                     /translation="MDERDLLTLVSAVTAIEEAASAVAREVERDRLRESSLSRLSTIE
                     AELNRSQLALEKIIQEETA"
     gene            complement(15778..16941)
                     /locus_tag="VFQ10_06885"
     CDS             complement(15778..16941)
                     /locus_tag="VFQ10_06885"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HET9965102.1"
                     /translation="MELAGLNVLDVFIALFVVVVILRGARTGFLAGVFSLVGVVVGVS
                     AGSRIASSLMAENGTPIYGAGITLGSILAFAVLGEVVARTIGGSIRNRLSSPTSETLD
                     GFGGAVLGFALSLVLVWAVGVFALQSPPLAGLHPVVRDSRILQSLDERMPSDLLTRAV
                     ADLEPLPQIRGPEPEVSAPEGSIVGDPDVRAASARTVRVGGIACGYGVEGSGWVAGRN
                     LVVTNAHVVAGETATHVQAGGTGRRLPAKVIVFDEKNDIAVLRVKGLGLTPLRLAAPR
                     PGEAAAVIGFPENGPLDIEPARTWRTQRVTSSDAYDRGLVERVVTSFRVYVRPGNSGG
                     PVVNEYGRVVSTIFASRTGSNNSGYGIPSRIVHRHLRMAADRTTPVSTEGCAN"
     gene            17133..18173
                     /gene="rlmN"
                     /locus_tag="VFQ10_06890"
     CDS             17133..18173
                     /gene="rlmN"
                     /locus_tag="VFQ10_06890"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET9965103.1"
                     /translation="MNAAEFLPDVRAVLDERNEPSYRIRQIYKALTGALVRDWREATN
                     LPRELQAALSEVAPAAALALERVTQATDGTRKYLFHTRDGHAIETVMIPEKARRTVCI
                     STQVGCPMGCTFCATGLLGIKRNLTAREIAEQVFVCARDIAPERVSNVVVMGMGEPML
                     NYDATLGALRVLNDPDGFGLAARHIAVSTSGLVEKVRRFADEPEQFHLAISLHTPFED
                     ERRQIMPVAALHTITELMEAARYFVARTRRKLFFEYTLLAGVNDQSRHVEALAELLDH
                     PLYHLNLLRFNWTDTGFSATSKAEAKRFLGHARELGLSATLRPSRGQDIDAACGQLAA
                     KDLRGRPAVHMA"
     gene            18177..18656
                     /locus_tag="VFQ10_06895"
     CDS             18177..18656
                     /locus_tag="VFQ10_06895"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET9965104.1"
                     /translation="MKGEPAERLDPRAITVWRITGILNMLPLLVGAGFAGWALSRSGG
                     VSLFFVVLPVLAVLALAGVVVGVVPGLRWRRWRYEIRPDEVDLQRGIFWISRTLVPLA
                     RIQHVDTRQGPLQRRFGLSTVIFYTAAGPNQIPELSTPAAERARDRIAELTREQDEL"
     gene            18646..20097
                     /locus_tag="VFQ10_06900"
     CDS             18646..20097
                     /locus_tag="VFQ10_06900"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET9965105.1"
                     /translation="MSSEKRLHPVAMLVGAIRTLRRWVSGLVFPGVVLLVSQGFSLWT
                     VTLFLLGLLVVAALAALWGFLSWQATTYTVTGNSFRLRQGVFQKNERTIPLEHVQSVD
                     IVQGIIPRAFGVVEVRIETAGGGASEPDASLAALDRDAAQTLRREIEGSRRESSEETT
                     GPTILRRISTRDLLLAGATSGQIGVAFSVLAIGFQFLDNFFPEDFVRRLVETLAPNWL
                     MVVLILVPAGLLLAWLLAIAGTVLAYSGFTLSREGDFLYIKRGLLERREATIPLSRIQ
                     AVRISEALLRQPFGLASLRVESAGYGEDAGVSTTLFPLLPRKEVEELLAGAAPEFAGA
                     PAIEPLPRRSLRRYVFRSTFLYLIFAFAAALVSYLVFQSALGLFGLFLIPPAAAYGWL
                     CYRDAGWAFQEDRLIVRHRSLGRKTAIAPRLHLQSRGMVSSPFQRRVRLATFLAEVAS
                     GSGGSVLRVKDLDRGAAETLVGDLGPGVSIEGA"
     gene            complement(20100..>21807)
                     /locus_tag="VFQ10_06905"
     CDS             complement(20100..>21807)
                     /locus_tag="VFQ10_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965106.1"
                     /translation="EARHEELRRLAEDHGAYKVSAKALREAEESYQVARDEAAGLAED
                     LEDLSGGEDLERAERELREEEEKLRELAAHHRGRANADEREARNVDKAREAIDSGAEE
                     HCPTCHREFESGEQAEISDTLKRQAAAIRRRASRETEEAEKLAASADKTAEKRKKVSS
                     NLNRWRELREMRVRADDRVAARLEVLGKARSGLEEIESRITGTEAPGDADLEASRARC
                     DRLRELRDARPQVKSLATEHSRLAQRVEELVEELEGLSGVSYDAEAHKAKREEKDRLE
                     RALGRVEELERRLARRPEVEKALQAACERREKAEATAEKLRREISALDFDESAYEASV
                     ERVTAAEERLSTLQDAREGLGSEWKDADYRIERTKAELRRFDDDRKLANEKAAAASRM
                     DEMDGLFTEFFRTLTARARPMLEVEASDLVRELTDGRYERMEFDDNYRVKLLDRFDDS
                     YSIERFSGGESDVASLSARVALSRIVASRGGNTLGFLVLDEVFGSLDAGRRNNVLLAL
                     ERLKRSFGQIFIISHVAEVQESALVDEVWMLEEDEEGKSTVRRVNTPMMPAPDAIASG
                     GP"
BASE COUNT         3779 a         7060 c         6878 g         4090 t
ORIGIN      
        1 tgaatgtccc tcctctgcct gcgctccccg agccgctacg cggcaagtct gtaatagcgc
       61 tgcgtggctg ttactgcggg gagtcgcctg gtgacggcga ggaactggtg cgtcccatgc
      121 gcgaggagct cggcgagccg atcatggacg ccttcgggct gatgccgtac gcggcgatgg
      181 actccataag catggacccg gtcgacccga tgggagcccg ccagcactcc gagatgctga
      241 gtgagctctc gcccgaggct atccaaacac tcgtagaggt agcaggcgcc ggttctggct
      301 cgcccctgat catgctcgag ctcaggcagc tcggcggcgc cctggcacgc accgccgaac
      361 atttgagcac catgggcaag ggcgactcca agttcatcat gaacgggatc gggccggcct
      421 tcacgccgga gatggcggac ggggtcgtaa cttacctcgc ccgcgtggcc gacgcgactc
      481 gctccttcca gaccggcgac acctacgtca actttatgga gctcgaaggc gcgagtgccg
      541 agagggtaaa ggcggcctac gcgccagagg acttcgaacg actcgtcgcc ctcaaggacc
      601 gctacgaccc acacaacgtc ttccgcttca accgcaacat cgccccctcc acggcggaac
      661 actaagggca ggaaagagga ccgggggccc agcggccccc ggtcgagatg atttatattg
      721 taagcaccct tatgggaggg atcttcagct agacgttgga gggtgagatg caagccgagc
      781 gcgaagcgca ggagaaagtg gccaggccgt tgcctgaaga taccctgtgg gtacgattcg
      841 tgttcgtgga ccacgctggc atccccaagg cgaaggccgt tcacagggac ggcttcgacg
      901 ggagggcgag ggccggggtg ggactcgcca aaggagtcct ggcgctcgat ccgagcggtg
      961 ctctgcaccc ggagtcgggc ttgagcccgg taggagagtg ccggctggtc cctgacctat
     1021 cgacgcttac agccctcccc ttcgcccgcg ggcaggcgat ggtctccgtg gacatgacgg
     1081 agcccgacgc gagcacgccc tgggagggct gcccacgtag cgccctcaag cgggtgctcg
     1141 aacggctctc agataggggc tacaggacgc tcgcctcctt cgaggccgag ttctacgtct
     1201 gggatgccga gggtccgctg gacaggacgc cgtacgcggg gagctacgcg ctgaccccgg
     1261 cggcggagtt cgtcgcggag ctggcacaga cgctggagga gatgggtatc cggccggagc
     1321 agtgccacgc cgaggtgggg cagggaaacc tcgagctctc cgttggggag gacgaagcgt
     1381 tagctgcggc ggacaagcgc gtgatggtac tggaggccat aaggggcgta gcccaccgga
     1441 tggggctcgg taccaccatg gccccgaagc cgtacctgga aggagcaggc aacggccacc
     1501 acctgcacgt gagcctctac gaagacaacg cccctgtgct cttcaacccc tcaggaacct
     1561 tgagcggccg tggctcgggc ttcgttgccg ggatactgga gcatctgccc gccgttatgg
     1621 cgttcagcgc accgtcgccc aactcctacc agcgcctggc ccctggcatg tgggcctcgg
     1681 cttacgccgc ctacggcctg gataacaggg aggcagccgt gcggctcgcc tcgcctgtgg
     1741 ccggcaggga gtccgcgacc gccaacgtcg agctcaagcc tgtcgacgtc acgtcgaacc
     1801 cctacctggc cctggccgct gtgctggccg ccgggatgga cggtatggat cgcggcctcg
     1861 accctgggga gccgacgatg gtggacccgg ccacgctcag cgatgaggag cgcgcctcca
     1921 ggggcatccc gccgctcccc gcatccccgg acgaggccct ggacgcgctg gaaggagatc
     1981 atgccctcaa agaggcactg ggcgaaccac tcgtgcgcac ccacgtggcc gtggctcggg
     2041 cgcaggccaa gatagcaagg gagcttccac cggaagaagt agcgtccacg gccgcgatgc
     2101 tctactagaa gacaggttac aggcatcggg catcagcaaa ttatctgtaa cctgcaacct
     2161 catgcctgca agaaaggagg cggtccggcg cttcggagag gtgtaggagg acagactctc
     2221 gggcctcaaa ggcaggcggt agtgctatga acggaggcgg cccatggaag ctctagacct
     2281 cacccggatc cctgtcgtcg ataaccactg ccacggccta ctgcgggacc aggccttcga
     2341 ggacattgcg agctggagac aggctttcac cgagtccacg gatcctggta tgccgcggga
     2401 ccacgtagcg agcactgcct tgtaccggcg cctcatcagg atgctggcct actttctcgg
     2461 ctgtgagccg gaagaggagg ccgtctactc agcccgcacc gagaaggacg gacgagaact
     2521 cacaggcgcg ttgttgcgtg ctgcgaacgt cgataccttc ctcctggaca cgggctttcc
     2581 gccacccgaa acagtgctac aggtagcaga gctgggagag ctcggaggct gccgcgcgga
     2641 gccgatgctg cggctcgaga tcctgatgga gagtctgctc gctgaaaagg attctctgga
     2701 ggacgtgaaa gaggcgctcg ccgccgaact ggacgacgtg cggagccgga gctacgtggc
     2761 gctcaagagc atcgccgcct acaggacagg cctggacatt cgggagtggc cgcgggagga
     2821 ggccgaagaa tcgttccacg aatataggcg cgtcaccgga gcaggtcctg cgcgcctcgt
     2881 ccacaaaccg ctccttgaca ccctgcttca cgtcgcgttc gcgcaggcgg cccggcagga
     2941 agtcccggtc cagttccacg tcggttatgg tgacgccgac accgaccttc tactgggcaa
     3001 cccgctctac ctgcggccgg tcctccagcg gcccgactat cgtggcttgc ccgtggtgct
     3061 gttacacgaa tgctacccct acaccaggca gggcggctac ctggccgccg tctacgagaa
     3121 cgtctacctt gacctatcct acggcatccc cccgctcggc tacggagaga tggtctcctt
     3181 tacccggcag gcgctcggcg tcgccccgac ctccaaacta gtgtacagct cagatggcat
     3241 cggggtgccc gaactacact ggatcagcgc catagacggg cggcgtgcga taggccaagc
     3301 gctaggagag atggtagcgc acggagaact cagcctcccc gaggccgaag ccgctggaga
     3361 gagcatccta cgcgataacg cgacgcgcct gtacagactc taaagcggat tgcagagcta
     3421 tcgacctaca gctgatcgcg cattcggcca ggagcgaaag catagcgccg aaatgctgac
     3481 acaacaggag ggtgcgtgac cggacgcaag aggttatcct acccgccagg gcctgatcga
     3541 ggctcgtaac cgaacccgga gggcgagtat tgtaccgaca gacgcggttg cttggtgtgc
     3601 tactcattct tcaagtgggt gcgatggtgg ggatcggcgt ctggttgttc agcaggatcg
     3661 actgggacag ggtaatagag ctcaccaaca agcagggcca gatccaggtt aaatccattg
     3721 atggggcgtt gttgaagcca ctcgaacagg cgatcctgta cttcatctac tttctcccgc
     3781 ccgcgatcct gcttcttctc gccgggctcg gtttcgtgtt acttcggcgc cggggatgga
     3841 tgctggcctc tgttgcgcag gcccttatca tgatggcctg cctgttcttc tataccgacc
     3901 cgcgaccatg gttcgcctac cctatcatcg cctactgcat cttgatgatc ttgtacctga
     3961 actcccaggg cgtgcggacc gtagtccact ccaggcggat gaccccggcg ggcggtagat
     4021 cggagacagc tcatggcgaa tagcgcaagc tcggaggtga gagacgcgga gaccttgcta
     4081 cgtcgcttcg aaccagtgat ccggttcacc aggggcgagt ggttctaccc gatggacgcc
     4141 gagtcttacg tcgacgcctg cagcctctgg gtccggcatc ctggtgagga cgccgtctgc
     4201 gtggtgccag ccggggatct caccctggag agaatcgccc agcagccgga ggacagtagc
     4261 ggcgccgtac actacctcaa gatccccact tccaaggagg acgagaggcg aggtctcttg
     4321 gcgcgcctgc gggcaaggcg ctcatataag aaccctgatt ggcctcggga gaagttccgc
     4381 gcaggcaggg gccgcctggc acgggtgggt tacatctcgc ggttggccga cgcgctcttc
     4441 tctttggtgt tgctggccag gggcagggtt cccggcggag acgtagaggg ggcgcgcgtc
     4501 ttgtatggcc gcctcatgga gaaagaagaa cgcttcaggt accacgggcg agtgatcaga
     4561 caggacggct ggatcgtctt gcagtactgg ctcttctacg cgttcaatga ttggaggagc
     4621 aacttcttcg gcgccaacga ccatgaggcc gactgggaga aggtgttcgt ctatctctcg
     4681 gaatcgggcg aggtacgccc ggagtgggtg gcctacgccg cgcacgagga gaccggcgac
     4741 gatctaaggc ggcggtggga cgacccggag ctcgagaagg tgggggagca tcccattgta
     4801 tacgtctgcg ccggctcgca cgcaagccgt tactccgctg gcgagtacct gaccgaactc
     4861 gcgttccccc tgccgcgggc gttcgccgcc accaggaggg ttgcgcggcg gttctggtac
     4921 ataacgctgc gccagtacgc cgaagagtac tccgcagaga agccctacga aaggttcacc
     4981 atacccttcg tggactatgg ccgcggggac gggctgtcgg tcggacctgg tcaggatatg
     5041 gagtgggacc ctccgcgtct tatgatcgat ccgcttcctt cctgggttgc gggttatcgg
     5101 gggctatggg gcctctacac ccgcgatccc ttcgaaggcg aagacgcccc ggcggggcca
     5161 atgtacgacc gcgataagac catcagcagg gagtggtacg accctgtggg ctgggccggg
     5221 ctcgacaagg tgccgacccg aacagaggaa ctggacgtca tcatcaagcg ccaggcggag
     5281 atcgaagaac gccgcgctcg ccttcgctcc gagatacagg agatgagcag tcggctgaaa
     5341 gcgctcggca tggacgccga agccacgcgc ggccgatccc acttgagcaa gctctacgag
     5401 acccagacgc ggcacatcga agtgctctcc aaagaagtag aggagatgcg cgcggagctc
     5461 tccgaagacg aagtcctcca cgagtcgctg agcgatcatg ccgaacacct gagaaacggc
     5521 gagcgttcac ccgtccacgc ccacttggct gacccagtgc gccccgtctc agaggcggag
     5581 acccacgccg gtagggtggc acagctgtgg gcttcgatca gcgtggccgt ggtgctgctc
     5641 gcgctcatcg tcatcttcgc ctttcagaga cagcacctgc tctccgcgct cgtcttcgct
     5701 atcgccatgt tcgcgtttat cgaggccggt ttcaggaggc gactcaccag gttcatagtc
     5761 agtgccaata tcggattggc gatcgtaggc gcccttgtcc tcctccaaca ctacttctgg
     5821 cagatcatcg tcctcgcggt cctggccgtc ggcctctaca tcttgtggga gaatctgcgg
     5881 gagctcggac ggtaacacct gaaaccggat ctacggaact ggcgtatgga aggggaaaac
     5941 cagggcgctc cagatattgt tcaggcgtcg agcacgacgt cggagagcgg actcccgatg
     6001 cagatgaggc gcaggccttg ctccagctcc tcgtcgccta tagcgaaggc catgtcctgg
     6061 tcgatctcac cttccaggca cttctggatg cacgtcgtgc atgtgccgct gcggcagtcg
     6121 taggggaggt ggaggccggc gtcctcggct tcttcgagga tgtactcgtc ttcggctacg
     6181 tctatggtga tgccgctcct ttggaaagtt actctgtgcg atctggccat gtccgctcct
     6241 gcgtactctg cgagcaagtc tcgtgtgctg gtctgttcta gcataccggc gggtcccact
     6301 tggcgaacta tgtgacggtt tccatcctgc gaggccgacg gacctctcaa acgacttcga
     6361 agttgcccac catcatgtcc tcgtgttcga ggttgtggca gtggaagacg tagcgtcccc
     6421 ggtagccgtc gaaacggatg agcacacgcg ccctgttgaa ggcctaggcc cggttcctcc
     6481 cgcctggctt gcggtagatg ttgccggtgt ggggcttttc ctgccttcca ggctctaagg
     6541 tttcctgaag ccccacacct gtagcctacc tctgctcgaa gtcctctcct ggagcgccgc
     6601 agacgggaca ctcctcagga ggctggacgc cctccaactc ctcgccgcag gtggtgcaga
     6661 ccattgtgac ggtcatcggc gggtgcgggg cttctccatt ctcggttgct attttctcgc
     6721 cggtgtcctt cgcgaggtct gcgatggaga ccttgcccgt tatgacgtct tccatcgtgg
     6781 tgcgcgcccc tgtgagcgag tcctcgacga gggcgaggtc tatgtgcgtc gtgccgcgct
     6841 cgcgggcgag cttctcggtc atgttgcggg agatgttgcg cataaagccc ttgggcgccc
     6901 gctccaggcg gtcgatggcg tcctgtgtcc agacaaattc tctggagccg ccctcgggta
     6961 cgctggcgcc ggccttctgg aaggcggagt ggtcgatggg gcacttggcg cccgaggtct
     7021 cctgtacctg ttgcttggcg tggctgacgg ggcagccgcc ggacttgacc tcgggggctt
     7081 cgcggccagt ctcctcacgg tactgctgct cgacgtattc gcgcgtgatc gtggtgtagc
     7141 ccttcttgag cgcgctcttc tcgacgcgcg ccttgacgcg tcgcttctcc tgccactctt
     7201 cgagtgagtc gagcagggcc gtggcgtcat cggtccagtg caactcgcgg ccatcgtaga
     7261 cctcggagag gttcaggttc tcgccctcta cagtcgtggc tatgtgggcg tccacagcgt
     7321 ggaagtgggt cggacccgag ccgcagacag ggcatttggc tggcttgccc ttcgccacgt
     7381 agcggcagac gtcgcattcg aagcggtcct ggccctgggc ggcgcgccgg atctcgtcct
     7441 gaacctccgc gctgacgccc tctatgtcgc cctggtcctc gctcttgccg aagccgccgc
     7501 gaccagcctc gaagatctcc tgcttcgccg cagaatcgct accgttcgcg ccgttgttat
     7561 gaccattggc tttgtcggcg gtacgctcgt cgaagtcttt gaagagggag tctatgggct
     7621 tgtcttcgcc catcgcttcg gccgcgcgca tctgatctcc gatggcgcgc atcgactcca
     7681 tagcgccggg ggggaggatg ctctttatga cctcgtcgat gacgcccgtt gttatgaccg
     7741 tgtagccctt ctcgatggcg tagcggatga tggcgccggt cgccatgccg acgacgaagc
     7801 ctgggatgcg gtccaggcgc tccagcgcct ccttcgtcca cgtcatgtgc tcttccgcgg
     7861 tgtagaggtc aggcgcctgg tattcgtagt tggttatgag gacgttcgtc ggcaggtagc
     7921 gcatcatgtt ctcggtgttg ccgccgatgt ccatctcgtc gtccgagtgg tagccgatgc
     7981 ggcccataac gaccatcgtc ggatttacct cgttcacgta cttcagcgtt tgctcgaacg
     8041 gcttgccggc gaggagcgta gtcttcaggt ctacgccttc gtcctcggct atcttcctgg
     8101 ctatctccag gtgcgccgtg tagatcttgg ccagtcccga gtcgatgatc tcctcgtgca
     8161 gcttctcctg ctccttgaac ctgaacacct tctgggcctt ggtcgagagc acgccggcta
     8221 tggaatggaa catcgcgtag tggaagtaag ggtcgaagac ggagatagcc tcgaccgtcc
     8281 ccccgaactc tcgtgcgagc tctatcgcac gcttcaaacc gcccagcgac ttggccgagc
     8341 cgtctacggc gacgacgatg tgctcgaagg gactacgatc taggtccttg acgatcaggg
     8401 tgtcggcttt ctccaagcgc cttacgacgc gctcggtgac ggtgccgagt accgtgtcct
     8461 tgacggcggc catgcccata gcgcccatga cgacgaggtc gaagtcgttc tggttgacgt
     8521 cctcgacgat gaccttgaag ttcttgcctt cgagggagcg cctcacgagc tcgacatcgt
     8581 acttctcgca gagtggctcc agcacgtcga tgtaggagtc ggtgattatc tccaggcctt
     8641 tggtgatgag ctggtcgtgg atcttgcgct gccgcttcat ctcgtcctcg tcgcggaact
     8701 cctcggggag cccgctctcc atctgccgaa agcggacgtc gtgcatcttg gcggcgtagg
     8761 cgtggcaccc agcgaccctg cccccgaact gccgcgccac gtcgacgccg atgacgcacg
     8821 cctggttgga gtagtccgag ttgtctaccg ggacgtagat ctctttgtac atataccccg
     8881 ttctcctagc ttcgcaggac gccgagaagt ctgtaattgc gtcgtgcgca gagaacggcc
     8941 cttctccgcg ccaacgtagc ttaccgttta gccctataca ccgcaaataa aagtgttcac
     9001 accgagggta caaaagcgaa agatcagttc tgcatcgctg gacaaccacc agtggttgtc
     9061 gcaagatggc caggagcgga gagagtgagg taagaaatgg atctggagaa gatggagcaa
     9121 gacgcgagag cggcgaaaga tagcagatct tacacggacg gagcggcctt cgtcgatggg
     9181 cgtttcttac ccgtcgggga ggcgcgcgtc ccgatcctcg actggggatt cttgcgttcg
     9241 gacgcgacct atgacgtagc ccacgtttgg cgcgggagct tcttccgcct cgaggaccac
     9301 ctcgaccgtt tcgagcgctc gatggagcac ctgcatctgg gtccgccgta tgaccggagt
     9361 gagatccggg acatcctaat cgagtgcgtg cgcctgagcg gactgcgcga tgcctacgcc
     9421 gagatcatct gcacccgtgg gatccccaga ccaggctcac gagacccgcg cgactgcgag
     9481 aatcgcttct acgccttcgt cgtgcctttc atctggatcg cagacccgga gaagcaagag
     9541 cgggggctgc acgctgtaat cggaagtaag caccgcatag cgccagagtc ggtggacccg
     9601 accgtcaaga actaccactg gcttgatctc gagaccgggc tcctcgaagc ctatgagcgc
     9661 ggtggggaga cggtgatcct ggtcgacgct gaggacaacg tcgtcgaagg acctggtttc
     9721 aacgtcttcg ccgtagaaca cggtactatc tccacccctg accgcggtgt cctggaaggt
     9781 atcacccgca agaccataat cgagctcgcc gccgaacacg gcatgcagcc cgaagcccgc
     9841 cccatcccgg ccggcgagct cgggcaagcg gacgaggtct tcctatcgag caccgctggc
     9901 ggggtcatcc cggttacgac cgtggacggc gaggccgtgg gtaacggggt gcccggtcct
     9961 gtgacccggc agttgcgcga cgcgtactgg gacctgcacg atgacccgcg cttctcccta
    10021 cctgtccact acgattgacc agccccgcct ccaggcggat cgaagcgctg gccggcggtg
    10081 tgaccgccat gaagcgctgt gcttgagccc agagcaagac gagccatcgc ctctcggccc
    10141 tcagcgaaca agagtctgac ttgctgaccc gctgatacag cgacttatct caggccggcc
    10201 ccgcgccttc caaatcaggg gtgacttcgc ccgcagatcc gtccacggtg atccgctgcc
    10261 ctgtactgat gcgacctgtg gcgtcgggca cgccgacgac ggcggggatg ccgtactcgc
    10321 gagccacgat ggcgccgtgt gacattggtc cgcccatctc catcacgagg ccgcctgcgg
    10381 tgaggaagag gggcgtccaa ccggggtctg tcgagggtgc taccaggatc tcaccgggct
    10441 ccaggctggc ctcacccggg tccaggatca cacgcgcttt ctgtgtcacc accccgcctg
    10501 aggccggcgt gccgcgcagg acaccatccg ccccggtcgc atggtgtgtc cctgcggtag
    10561 gttcggtacc gtcggagagg aggaggcgcg gcacgtgtct gcgacctaac tcctgctcgt
    10621 agacggcgcg gcggtcgcgg actaagggac gaaggtcttc gcctgccagg gcagcccacg
    10681 cctctggcac ggagaggaag aagatgtcac cggcgctctg caggtgccca gcctccgcca
    10741 gctctacacc gacggtccac agcagctctc tggcgcgtgc catgagcaga acgatgcaga
    10801 acttgggcat ctcccgcagt cctgataggg ctctggcgcg tcccagtaga aacccaacca
    10861 gcgccccgcg cagacgtccc tctctggccg cgcggcgggt caactcgccg accatctctt
    10921 cggcctcgcg tgtcgcccgt ccgaactgta cgtcgggggc tgcttgcggg tcgtccaggc
    10981 gcaggtagtt cgccaacacg ccaaggatgt acgtcgggtc ctccgaccag cggggcaggc
    11041 cgaggtcgat ctccgccacc ccgcgatgcc cgtagaggcg taggaaatct gccagactcg
    11101 tctgcagctt cgggggcaga gagccgcgac gatagtcttc tgccagtcgc tccggcgggg
    11161 tttcacccaa cattctggcg atgtccgggt ctgcacgcgc ctctttggcg agcgcccaga
    11221 gcgccaggtc catctccgtc gtcgggttgt gcgggagcgc gcgcatcgcc acccgccttt
    11281 cgtcgtcggt ggcgagacct cctagtagct ttccggcgag gacgttggca gcgaggccgg
    11341 cggcgaagac cgggggtacg ttgggcagca tgtccggcgg accgccgagc agcaggcgct
    11401 cggcggctga gaggcgctcg gcggcgctag ctttagggga tacgtggccc gccgcgcggg
    11461 agcgcgcctc gacacgcgcc gctcgggccc tcgccttctc cgggcgtgcg agcgcttcta
    11521 cgaagcgtag cgggatgcgt cctcgcacga aaaccgagag gacggcgtgg aggacgggcc
    11581 gaatgggtgt cggtactggg gacaggcgag ggtctgccgc caggtgcctg acgataggga
    11641 ctgtccgtgc ttccatgttc cgcatcactc ccctgaagag cgcgcgtcct tgcttgctgc
    11701 gcagcacggg cgttatgtcc gcgaagatgc gtccggcgga ctcggtgaag aaggccgcgc
    11761 cttcgtaggg gttgctcggt gcacggcctg caagcatggc cagcgccgag gttatgaggc
    11821 ggaacccctg gatgcccatc ggggtcagcg ggcggtagac gccctgtgcg acgctgaagg
    11881 agaagtacac gcgtaggtcc tcttcgctcc tcgtagctct ctccggtagg gggaagagtg
    11941 tggtgatcgg acgtgcctgt agaagccaga tcccaccgtc atcgtcgatc gcccactcgg
    12001 tatcctgcgg catcccataa tgctcctcga cccgctcccc cagcttcgcc agtgccagga
    12061 cctgaccgtc cgagagggaa gcgctgtctt cgcccgcatc gagtacgacc cgctcggtgc
    12121 cgctacccgg gccggccatg atggcgacac gcttgtctcc caggcggcgc tcgactatct
    12181 ctacggtcgc agtgttcgcg acgaagtggt ctgggttgac catccccgag acgacagcct
    12241 cgccgaggcc tgggctggcg tctatgactg cctgacggcg cttccccgtg accgggttcg
    12301 cggtaaacag tacgccggct accgacgcgt cgaccatcct ttgcacggcg acggcgaggc
    12361 gcacgctgtg cgggtcgatc ccgttcgtag cccggtagct gacggcgcgg tccgtccaca
    12421 gcgaggccca gcagcgccgc accgcgtcga gcacggactc gacgccgatt acgttcaaat
    12481 acgtgtcctg ctggcctgcg aagctcgcct ggggcagatc ctcagccgtg gccgaggaac
    12541 gaacggcaac agggacaggt gcaccgtctc ccaatccctc gtatgcctcc gcgatggcct
    12601 gtcggagaag gtcgggaacg attgcatccg agaggtaaga tctcgcctgc ttggcgagcc
    12661 cagccagacg cgccgtgtcc tcgggctgcg tcccagccag cgcggcgagc gtgggctcca
    12721 gccccgcatc tgccgccacc agatcgtagg ccgctgtggt aacacagaat ccggccggca
    12781 ccggaaatcc agcccggacc agatcgccga ggttcgccgc cttcccgccg gccactgcca
    12841 gcgcacccct gtctatcgcg gtgaagggta gtattagccc agggtaaccg ttctcctgct
    12901 cgttcatctg tttctccttt tcgagtttgg aatgtcgaat ctgggatcta gaattgttcg
    12961 cttgctccac acatttcccc aattcctact cgacctcccg cagtgtgagc gaggcgagca
    13021 ggagaagcgc cacgctgtag gctacgagta cggcgaggct cccctgcacg tcctcgaacg
    13081 tccagcctga ttcgataacg tcctggatca catcgttggc ccagaagagc gggaatgcat
    13141 gcccgagcca gtcggcccag ttcggcaact cgtccacgtc tacgagcagg cccgagagga
    13201 agacggaggg gactatgatg agcggcacaa aagggaagac ctggccttcg tggcgggcga
    13261 acgtggagat gaagatgccg agcatcacgg aggctatggc caggagccac acgaccacga
    13321 acagcatcgc cagcgtctcc tcgtcgtagt ccagctcgaa gagccagatc gcctcggcca
    13381 gcccggcgac ggcctggaag gtcgcgagcc ccagaaagcc aagaacatac ccggcgatta
    13441 tctccatgcg ccggaagccg tttacgaaca ttctctccat cgtgcccatc gtcctctcct
    13501 gtacgagcga gattgcgcag agcaagaagg ccaaaaagtg gaccaggaag gccgccaccg
    13561 gcacggcgta ccgcgccacg tcgaaggtct ctggcaaagt gtcgaagaag atcttcagga
    13621 ggtaaacgat cacgagcggg gccgccacgg agagcacgag gaaccttgga ttcctgaaga
    13681 gttgcctgac caccctccct gcgacgagca acgtccggcg cattacccaa cctcctgttt
    13741 ctcgatcagg gacaggacga ccgcctccag tgagtccgtc ttcacgtcga cagcctttac
    13801 gtcaggtcgc aaaccatagg ggcgaagcgc ggcggccaga tcctcgggat gcccgccgat
    13861 ggtttcttct tcctctctgc cgtcttgctt taccgtaagc ctggtcctgc cccgttcgag
    13921 tatgctacgc ggagtgtcgg tggcgatgat cctgcccttg aggagtatcg cgatgcggtc
    13981 gcagagcatc gcctcgtcca tcaggtgcgt gcttataaac agggtcgtgc cgcctccggc
    14041 tagctccctg aaagtcttcc acaaccggct cctcagttgc gggtccacgg ccgccgtggg
    14101 ctcgtcgagg aagatcacct ctggcccgtg gacgagggcg caggctagcg agacccgccg
    14161 cttcatcccg ccggagaagg tgtgcacagg gtcgtaggcc cttcctgaga ggtcggtaaa
    14221 atgcaagacc tcatctacct tcttcttgag atccggcgtc cggtgtgcag cgccgaagaa
    14281 ccagacgttg tccctggcgg agaggtcttc gtaaagcgcc gcgccctggg gcatgtatcc
    14341 aatcctttgc ctcaactcgg ccctttcctc gagcgggttc aacctcatca cccgcgcgat
    14401 gccgccggag ggccgcagcg ccccgactag acctttgatc agggtcgtct ttcctgcccc
    14461 gttcggccct accaaaccga acacctcgcc ccgtctcacc gagaggtccg tgccccccaa
    14521 cgcttcgacc cggccgtaca tcttggtcaa tccggagacc tcgatggcat tcaccctttg
    14581 tcctctttcc cttcgagacc ccgcaagaag atgccggcca ctgcttctat gtgctcctcg
    14641 tcggtgaggc cgtcttccct tatggccgcg aagaacatct tgcccccagc ctgggggaga
    14701 agcattccta taaacgccct ggcgctcgcc cgcacgtcgt gcggccttag cctgcccagc
    14761 tcgacctggc gcgccatgta gctcttcagg aacccgagca ccctcttgat caccgcgttg
    14821 ccgaacacct cagagacctc gggccgcctc atggcttccc caacgagcag ccgcgccatc
    14881 ctctgggtgg cgcgatcgtc gaacgtcgag aggtacctcc tggcgatgtt cggcaacacc
    14941 tcttccggtg gaaggtcaag aagcctggca gggtccctta ccgcacgcag aacagggatc
    15001 cttgacccca gaacctccct gaaaagggcc tccttgtcag ggaagtacca gtagatgagc
    15061 gccggagatt gcaaactcgc cgtccgcgca atgctcttga tcgtcgctcc cttgaagccc
    15121 ttcgcagaga actcctcgaa agccgcctcc aggatctgcg cccgccggtc cgactcctca
    15181 gtcacaatac ctctttattg atcgtttaat caacgactat actcttgata gaacgttcag
    15241 tcaagactgg ctgccattct cagcctcatc gttgcggggc acggccagat ctacaacggc
    15301 cagattggca aaggtgtgat ctacatcctc atccagctca taaacggtgc gctgacagcc
    15361 gttctgatcg gctgggtcct actccagatc gtgggccttt gggccatgat cgacgcctac
    15421 acgaccgcga agcgcaacaa ctagcggtac ggcttcaggt aatatccgtc ctatggacga
    15481 acgagacttg ctaacactag tgagcgccgt cacggccatc gaggaggcgg cttcagccgt
    15541 tgcccgcgag gtcgagcgtg accggctgcg cgagagctcc ctctcccgcc tctcgacgat
    15601 cgaggccgaa ctcaacagga gtcagctcgc gctggagaag attatccagg aagagacggc
    15661 gtaaagcatt tcagccagtc agcaagtcag cacgctcctt tcgggagctt tcagcccgct
    15721 acgcccttcg ggcttcgctc tcagcacggc ggcgaagccg gagcgggctg acttgctcta
    15781 attggcacat ccctcggtgc tgacgggggt ggtacggtca gcggccatcc gcaggtgacg
    15841 gtggactatt cgggatggga tcccgtagcc tgagttgttc gagccggtgc ggctggcgaa
    15901 gatggtcgag acgacccgcc cgtactcatt gacgacgggg ccgcccgagt tgccaggtct
    15961 cacgtatacg cggaaactgg tgacgacgcg ctccaccagg ccacggtcgt aggcgtcgct
    16021 agaggtgacg cgttgcgtcc tccacgtgcg ggcaggttcg atgtctagcg gaccgttctc
    16081 ggggaacccg atcacggcag cggcttcgcc gggcctgggt gctgcgaggc gcagcggcgt
    16141 caggccgaga ccttttacgc gcaggacggc tatgtcgttc ttctcatcga agacgatgac
    16201 ctttgcaggc aggcgccgtc ccgtcccccc ggcctgtaca tgcgtcgccg tctcccctgc
    16261 gacaacgtgg gcgttcgtga cgaccaggtt ccggcctgcg acccagcccg aaccctccac
    16321 gccgtagccg caggctatgc ctcccactcg caccgtgcgc gcactcgccg cccgcacgtc
    16381 gggatctccg acgatgcttc cttcgggggc tgatacctct ggttcagggc ctcgtatctg
    16441 aggcagcggc tccagatcgg cgacggcccg cgtgaggaga tcggagggca ttcgttcgtc
    16501 gagggactgc aggatcctgg agtcccgtac cacagggtga agccccgcga gggggggtga
    16561 ctggagggca aagacgccga cggcccacac cagcacgagg gagagcgcaa accctagcac
    16621 cgctccccca aatccgtcca gcgtctcgga agtcgggctg gagagacggt tcctgatcga
    16681 acctcctatc gtcctggcga cgacctcccc gagcacggcg aaggcgagga tgctccccag
    16741 ggttatacca gcgccgtaga tgggggtccc gttctcggcc atgagggagg aggcgatcct
    16801 tgaccccgcg gagacgccga cgactacgcc aaccagcgag aagacgcctg cgagaaagcc
    16861 cgtcctcgcg ccgcgtagta tcacgacgac cacgaagagc gcgataaaca cgtccagaac
    16921 gttgaggccg gcgagctcca ttgacagatt ctacctccgg tgcaagcctt ttactgccct
    16981 tggccgccca tgcccgagct cgccgacccg tgtttacccg gtgagacggc atcctggcaa
    17041 tagcgttacg aagaggtcca gtaggggaaa tcgcgttcgc gactgccctg gcgattttgt
    17101 gtatgggcgg gcgccggtat aatctctcca gcatgaatgc ggcggaattc ttacccgatg
    17161 tgcgggcagt cctcgatgag aggaacgagc cgtcctaccg catccgccag atctacaaag
    17221 ccctcaccgg tgcgctcgtg cgcgactgga gagaggccac gaacctgccc cgggaattgc
    17281 aggcggcgct ctctgaagtg gctccggcgg ctgccctggc actggagcgc gtcacccagg
    17341 ctaccgacgg tacgagaaag tatctgttcc acacccgcga cggccacgcc atagagacgg
    17401 tcatgatccc cgagaaggct cggcgcacgg tatgcatctc tacacaggtg ggctgcccga
    17461 tgggctgcac gttctgcgcg acggggctgc ttgggatcaa gaggaacctt acagcgcggg
    17521 agatcgccga gcaggtcttc gtctgcgccc gagacatcgc accggagcgg gtatccaacg
    17581 tggtggtcat gggtatgggc gagccgatgc tcaactacga cgcgacgctc ggggcgctga
    17641 gagttttgaa cgaccccgac ggcttcgggt tggcggcgag gcacatcgcg gtctcgacca
    17701 gtgggttggt agagaaggtc cggcgcttcg cagacgagcc cgagcagttt cacctggcga
    17761 tctcactcca cacccccttc gaagacgagc ggcgccagat catgccggtg gcagccctcc
    17821 ataccatcac cgagctgatg gaggccgccc gttacttcgt agcgcggacg cgccgcaagc
    17881 tcttcttcga gtacacgttg ctggccggtg tcaacgacca gtcgcgccac gtcgaggctc
    17941 tggcggaact tctcgaccac ccgctctacc acctgaacct gctccgcttc aactggacgg
    18001 atacgggctt ttcggccact tccaaagcgg aggccaagag gttcctcggg catgccagag
    18061 agcttggcct ctcggcgacg cttcgtccca gccggggcca ggacatagat gccgcctgcg
    18121 gccagctcgc cgcgaaggac ctgcgcgggc gacccgccgt tcacatggcc tgacccgtga
    18181 agggcgaacc cgcagagagg ctcgatccgc gggcgatcac tgtgtggagg atcacgggca
    18241 tcctcaacat gctgccgctc ctggttggag caggtttcgc tggctgggcg ttgtcgcgct
    18301 ccggtggcgt gtccctcttc ttcgtggttc tgccagtgct cgcggtactc gcgttggccg
    18361 gagtggtcgt cggggtggtg cctggcttgc ggtggaggag gtggcgttac gagattcgac
    18421 ccgacgaggt ggacctgcag cgcgggatct tctggatcag ccgcacgctc gtgccgctgg
    18481 cccgcatcca gcacgtggac accaggcagg gaccacttca gcgccggttc gggctctcga
    18541 ccgtgatctt ttacaccgcg gccgggccga accagatccc cgagctctcg acccccgccg
    18601 ccgaaagagc gcgggaccgc atcgccgaac tgaccaggga gcaggatgag ctctgagaag
    18661 cgtctccacc ccgtggccat gctcgtcgga gccataagga cgcttcggcg ctgggtcagc
    18721 gggctcgtct ttcccggcgt agtcctcctc gtgagccagg ggttcagcct gtggacggtg
    18781 acactcttcc tgctcggcct cctcgtcgtg gcggcgctgg cggcgctctg gggctttctc
    18841 tcctggcagg cgacgaccta cacggtcaca ggcaactcct tccggttgcg ccagggcgtc
    18901 ttccagaaga acgaacggac gataccgctg gagcacgtac agtcggtgga tatcgtgcag
    18961 gggatcatcc cgcgcgcctt cggcgtcgtc gaggtacgga tcgagacggc gggcggaggc
    19021 gcgagtgagc ctgacgcctc cctcgctgcg ctggaccggg atgcggcaca gacgctgcgg
    19081 cgtgagatcg aaggttccag gagggagagt tctgaggaga ctacaggccc aacgatcctc
    19141 cggcggatct cgacgcgcga cttgctgctc gccggggcca cgagcggcca gatcggggtc
    19201 gcattctcgg tgctcgccat cgggtttcaa ttcctggaca attttttccc ggaggatttc
    19261 gtccggcgcc tcgtcgagac cctcgcgccg aactggctca tggtggtact gatcctggtg
    19321 cccgcgggcc ttctactggc ctggctactt gcgatagccg gaaccgtcct cgcctactct
    19381 ggtttcacgc tctcccgcga gggggacttc ctgtacatca agcgcggtct tctggagcgc
    19441 cgcgaggcga ccataccgct ctcccgcata caggccgttc gtatctcgga agctctcctg
    19501 cgccagcctt tcggcctcgc ttccctgcgt gtggagagcg ccggttacgg cgaggacgcg
    19561 ggtgtctcga ccacgctctt tcctctcttg ccccgcaagg aggtggagga actcctggct
    19621 ggtgcggccc ccgagttcgc cggcgctcca gccatagagc cgctcccgcg ccgttccctc
    19681 cggcgctacg tattcaggtc cacgttcctg tacctgatct tcgccttcgc cgccgcgctg
    19741 gtctcctacc tcgtcttcca gtccgctctg ggcctcttcg gtctcttcct gataccaccc
    19801 gccgccgcgt acgggtggct atgttaccgg gacgccggtt gggcgttcca agaggaccgg
    19861 ctgatcgtgc gccaccggag cctgggccgc aagacggcca tcgcacctcg acttcacctg
    19921 cagtcgcgtg ggatggtaag cagtcccttc caacgtcgtg tacggctcgc tacttttctt
    19981 gccgaggtcg cctccggatc gggcggatcg gtccttcggg tcaaggacct cgacagggga
    20041 gccgccgaga ctctggtggg tgatctggga ccgggagtca gcatcgaagg tgcgtgaggt
    20101 tacggaccac cggaggcgat ggcgtcgggc gccggcatca tgggggtatt cacgcgcctg
    20161 acggtgctct tgccttcttc gtcttcttct agcatccaga cctcgtcgac gagggcagat
    20221 tcctgcactt cggcgacgtg ggagattatg aagatctggc cgaacgagcg cttgagacgc
    20281 tcgagcgcga gtagcacgtt gttgcgtcgc ccggcgtcga gactcccgaa aacctcgtcg
    20341 aggacgagga aacccaatgt attgccgccg cgggaggcca cgatcctcga gagcgccacc
    20401 ctcgcggaga ggctggcgac gtcggactcg ccgcccgaga agcgctcgat agaataggag
    20461 tcgtcgaagc ggtcgaggag cttcacccgg taattgtcgt caaactccat ccgctcgtag
    20521 cggccgtctg tgagctctcg caccaggtcg gacgcctcga cctcgagcat gggacgcgcc
    20581 ctcgccgtca gggtgcggaa gaactcggtg aacagtccgt ccatctcgtc catgcgcgag
    20641 gcagcagccg ccttctcgtt ggccagcttc ctgtcatcgt cgaagcgccg cagctcggcc
    20701 ttcgtccgct cgatgcgata atctgcgtct ttccactcac tcccgagccc ctcccgcgca
    20761 tcctgcaacg tcgatagccg ctcctcagcc gccgttacgc gctccacgga agcctcgtag
    20821 gccgactcgt cgaagtccag agcggagatc tcacgccgca acttttccgc cgtagcctcc
    20881 gctttctctc tccgctcgca ggctgcctgc aacgctttct cgacttcagg acgcctcgcg
    20941 aggcgccgct ccaactcctc gacccggccg agtgcccgct ccagccggtc tttctcctcc
    21001 cgcttcgcct tgtgagcttc ggcgtcgtag gagaccccgg agagaccttc gagctcctcg
    21061 acaagctcct cgacgcgctg tgcgagtctg gagtgctcgg tagcgaggct ctttacctgc
    21121 gggcgggcgt cacgcaactc cctaagacgg tcacagcggg cgcgggacgc ctcgaggtcg
    21181 gcgtcgccgg gggcttccgt gcctgtgatg cgggactcga tctcctccag gccggatctg
    21241 gctttcccga gcacttcgag gcgggcggcg acccggtcat cggcgcggac tctcatctcg
    21301 cgcaactcgc gccagcggtt taggttcgag gaaacctttt tccgcttctc ggcggtctta
    21361 tctgctgagg cagcgagctt ttcggcctct tcggtctcgc gggaggcgcg acggcggatc
    21421 gcggccgcct gcctcttcag ggtgtcggag atctccgcct gctccccact ctcgaactcc
    21481 ctgtggcagg tgggacagtg ctcctccgcg ccgctgtcga tggcctccct ggccttgtcc
    21541 acgttcctgg cttctcgctc gtcggcgttg gcccttcccc tgtgatgcgc cgcgagctcc
    21601 cgcagtttct cctcctcctc acgcaactcg cgctccgccc gctccaggtc ctcaccgccc
    21661 gaaaggtcct cgagatcctc cgccagcccg gcggcttcat cccgcgccac ctgatacgac
    21721 tcctcggcct cgcggagggc cttcgcggag accttgtagg cgccgtgatc ctcagcgagc
    21781 ctccgtagtt cttcatggcg ggcctcc
//
LOCUS       DASTKM010000166         5189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1101629, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000166 DASTKM010000000
VERSION     DASTKM010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101629"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFQ10_06910"
     CDS             <1..523
                     /locus_tag="VFQ10_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965107.1"
                     /translation="TANANAALLEAQGLDIRAVSGAAAPEILEYRFQQNVLDKLESVA
                     ERLPQVVQLGENGGQPIDFLAIARQMIGTGGEPLFTSEDMKKIRERVEEIQERIRGRE
                     QEIEDVVSAEQGVTEEEPQAPQPAEEENAERLEEIRQSVTDKVVKERVERRLQSANGG
                     ADSERAENEEETE"
     gene            520..1818
                     /locus_tag="VFQ10_06915"
     CDS             520..1818
                     /locus_tag="VFQ10_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9965108.1"
                     /translation="MTAPSVPEFSKIKEVVTGWSGVRRYLRGGEEGAIVPVVIPKDRR
                     RYGWLFLLGLAVYTLGVAIFIGNGRLTALAILATIFFVALGALWLWRSAIVEIEQGTT
                     GVLSSYGRITGTLTPGRHYLWWPWQKVEYVVDTATEIPYLAPVAVCPTQENVPLKAIE
                     FFLKFRIQEPIDFVRTIGASNFDLVLSSAVQDAIRQRSRQVRTERAYDLRGGDVGDMQ
                     DYLNRQLERYGVRILGANIPDVQLPDQYQQHLATRERLAKELAAYEREWELTRKRRID
                     QLLMEIERSKKERDARLIEVKEARNKARQDVARMLEEKETEAQRVRLEIETRGRATLT
                     EAENEATALRRLGQSHRDNRAMVQYELAFRRVEVGERLMQHAPRPLLVRGDGGESSPL
                     SMLLLARMLPQLADNNGAPLEDAREVMREARRNFGEEGEA"
     gene            complement(2293..2880)
                     /locus_tag="VFQ10_06920"
     CDS             complement(2293..2880)
                     /locus_tag="VFQ10_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9965109.1"
                     /translation="MSSSAIFRPAIREDSRKIAELFAIASGGVAEYVWGTLAPQYPGL
                     TPLEIGARRYAGEESAFSYKNCTVAELGGEVIGMLHAFLMEEEPEATDEPMDPILEPY
                     AMLEVPGSYYVSAMAVFPEHRGKGLGTRILEVAKERARHSGRGQVSLLVFECNKGAVE
                     LYRRNGFEVIDRAPVVPHEVISYTGEVLLMTAAAE"
     gene            3096..3296
                     /locus_tag="VFQ10_06925"
     CDS             3096..3296
                     /locus_tag="VFQ10_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965110.1"
                     /translation="MLLATGFFVGVVLRSVLLGVLLFALGAILFAIRGYLNTGDKAVA
                     GALIFVALVAISIEVIVYSLGP"
     gene            complement(3326..3715)
                     /locus_tag="VFQ10_06930"
     CDS             complement(3326..3715)
                     /locus_tag="VFQ10_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965111.1"
                     /translation="MTTSHPYKDAGGRPSHCRVRIYLPDDVLDAPVVVCSELPNNPGG
                     SITNSPEVIAAGVIRANELPTPLVWIEHWPEESTDVEETFEVVVFSSYEVVERTPYLG
                     QSRAWIGDATWKRLDRATVEVLVGGKV"
     gene            complement(4835..>5189)
                     /locus_tag="VFQ10_06935"
     CDS             complement(4835..>5189)
                     /locus_tag="VFQ10_06935"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9965112.1"
                     /translation="SIVVRCYEFDRMLAFWREALHYVPREPAQEGWVVLRDPEGRGPN
                     VSLERFPEPLERIGKTSRVHLDLYTNDQDGEVERLVGIGARRYPQEYSLDDDFIVLED
                     PDGNRFCVVQAPEGG"
BASE COUNT         1052 a         1540 c         1603 g          994 t
ORIGIN      
        1 cacggccaac gccaacgcgg cgctgctgga ggcccagggg ctcgacattc gggcggtcag
       61 cggcgcggcg gccccggaga ttttggagta ccgcttccag cagaacgttc tggacaagct
      121 ggagtcggtc gcagagcggc tgccgcaggt ggttcagctc ggggaaaacg gaggtcagcc
      181 cattgacttc ctcgccattg cccggcagat gatcggtacg ggcggcgagc cgctctttac
      241 atcggaggac atgaagaaaa tccgggagcg ggtggaggag atccaggaac gcattcgggg
      301 acgggaacag gagatcgagg atgtagtctc cgctgagcaa ggcgtgaccg aagaggaacc
      361 ccaagcacca cagcccgcgg aggaggaaaa cgcggagcgg ctggaggaga tccggcagtc
      421 cgtcaccgac aaagtggtga aggaacgtgt ggagcggcgt ctccagagcg ccaacggcgg
      481 ggccgatagc gaaagagcag aaaacgagga ggagacggaa tgaccgcgcc gagcgtaccg
      541 gagttctcca agataaaaga ggttgtaact ggttggtctg gagtccgacg ctacctgcgc
      601 gggggtgagg aaggcgcgat cgtgccggta gttatcccga aggaccggcg gcgctacggc
      661 tggctattcc tgctcggcct ggccgtctat actttgggcg tagcgatctt tatcgggaac
      721 ggcagactta cggcgcttgc catactggcg acgatcttct tcgtggctct aggcgctctc
      781 tggctttggc gaagcgccat cgtggagatc gaacagggca cgaccggcgt cctttcgagc
      841 tacggcagga ttaccggcac gctcacaccc ggtcgccact acctctggtg gccgtggcag
      901 aaggtcgagt acgtggtgga tacggcgacc gagatcccct atctggctcc tgtagccgtc
      961 tgcccaacac aggagaacgt accgctcaag gccatcgagt tctttctcaa gttccgcatc
     1021 caggaaccca tagactttgt ccgcaccatc ggggcctcca acttcgacct cgttctctcc
     1081 agcgcggtgc aggatgcgat ccggcaaagg agccggcagg tccgcaccga gcgggcctac
     1141 gatctgcgcg gcggcgacgt gggggacatg caagactacc tcaatcgcca gctcgaacgc
     1201 tacggcgtcc gcatcctcgg cgcgaacatc ccggacgtgc agctcccgga tcagtaccag
     1261 cagcacctcg ctacgcgcga gcgcctggcg aaggagctcg ccgcctatga acgcgagtgg
     1321 gagctgaccc gcaaacgtcg catagaccag ctcttgatgg agatcgaacg ttcgaagaaa
     1381 gagcgcgacg ctcgtctgat agaagtcaag gaagcccgca acaaggctcg ccaggacgta
     1441 gcccggatgc tagaggagaa agagaccgaa gcccagcggg tgcgacttga gatagagacc
     1501 cgcggacggg ccacactcac tgaggccgag aacgaggcga ccgccctgcg ccgcctggga
     1561 cagtcccacc gggataaccg ggcgatggtc caatacgagc tggccttccg tcgtgtcgag
     1621 gtcggcgagc ggctaatgca gcacgccccg cgtccgcttc tggtccgcgg cgacggcggc
     1681 gagtcctctc cgctttctat gcttcttctc gcccggatgc tgccgcagct cgcggataat
     1741 aatggtgcgc cgctcgaaga cgcccgcgag gttatgcgcg aggcgagacg caacttcggc
     1801 gaagagggtg aagcctaagc ttattttaca ttcggagaat cttgtgatgg gcttgaaccc
     1861 gccacaagat tctccttgca actcaccgtc acgagcgtca tcatcgtcac accactcaga
     1921 gaaccgcata gagaagggct tttctctgtg acgatagatg gcgcttccca gtaaaggacc
     1981 gttacgcaaa aaccctctag agatgagcgg tgtgtgacga ctgtgacggt cgtgacgatc
     2041 aaatgcagag gttctctaaa ggcggggcgg cactctcttc ggaccgaaag gtaatgtgcg
     2101 ggtgggagca tgggagcgca agcgcgatgc aggtccacgg ccctaacgtt tctccaaata
     2161 cccccactgc tgctcgtagt attcgctgtc ccctcagctt tgaggagtcg ctgcaaaaaa
     2221 ccgtggccta ttaggagggt gaccgactgg gctaccgccg agcgggctac tgctgggcgg
     2281 cgacgcccga cgttattcgg cggcggccgt catcaacaga acctcgcccg tgtagctgat
     2341 gacttcgtgc ggcacaaccg gggctcgatc gattacctcg aagccgttgc gccggtagag
     2401 ctcgaccgcc cccttgttgc actcgaagac tagcaggcta acctgcccgc gcccactatg
     2461 gcgagcccgc tcctttgcta cctcgagtat gcgcgtcccc aggcccttgc cgcggtgctc
     2521 ggggaagacg gccatggcgc tcacgtagta gctgccggga acctccagca tggcgtacgg
     2581 ctccaagatg gggtccatgg gctcatcggt tgcctcgggc tcttcttcca tcaggaaggc
     2641 atgcagcatg ccgatcactt ccccgccgag ttctgcgacg gtgcagttct tgtaggagaa
     2701 ggcgctttct tcgccggcgt agcgcctggc accgatctcg agcggcgtca ggcccgggta
     2761 ctgcggggcc agcgtgcccc acacgtactc ggccacgccc cccgaggcga tcgcgaacaa
     2821 ctcggcgatc ttgcgagagt cttctctgat cgccggcctg aagatcgccg aggaactcat
     2881 agcttatcca ccgccatgga tcgtattcta caagagccta acggcgggat tagagtggca
     2941 cgtaccgcgg caaatcatcc cgccacgcct taggaaggga gttacggcgg aatgattgct
     3001 agtgaggcca tgatgggtag ataagcgttg tgcgagagtt tgaagacaga catgccccac
     3061 ggtgggtaaa gtcgggcttg agcgtgggat caggtttgct gttggcgacc ggtttcttcg
     3121 tgggtgtggt actgaggagc gtgctgctag gcgtgctgct gttcgccttg ggagccatac
     3181 tcttcgccat ccgcggctac ctcaacaccg gtgacaaggc agtggctggg gctttgatct
     3241 tcgtcgccct ggttgctatc agtatagagg tcatcgtcta ctccttggga ccatagcagc
     3301 cgtactactc tgggtagcca ttttcctaca ccttccctcc aaccaacacc tccacggtag
     3361 cgcggtctag cctcttccat gtagcgtctc ctatccatgc ccgcgactgc ccgaggtagg
     3421 gcgtcctctc tacgacctcg tagctagaga agacgaccac ctcaaacgtc tcctctacgt
     3481 ccgtgctctc ttcaggccag tgctcgatcc ataccagcgg ggtgggtagc tcgttggctc
     3541 ggatcacccc cgcagctatc acctccggtg agttggtgat cgagccgcca gggttgttag
     3601 gtagctccga gcagacaacc acgggagcgt cgagaacgtc gtcgggaagg tagatgcgaa
     3661 cccggcagtg ggagggacgt cccccggcgt ccttgtaagg gtgggatgta gtcacttgtc
     3721 aggtgcatcg gcatcgttcc ggcagaagga tacatgtaga gtgatcatag gccatcagta
     3781 aagctgacca gaataccgac cgcaccgacc acaataacta gtggggagga gaaagccctg
     3841 caaaacggct catttccagg cggtcgataa gcgggcggta ctttcgagag cggtcggtaa
     3901 tggttcctgg tagtcgctaa acgatgcttg gtaccgaccg caagaaaacc ctgcaaacgg
     3961 ccgcattacc aatagcgcgg gcgatgcggt cggtattttg cgacgcttat tgagaacatc
     4021 gctctcttct ttcgcggcct ccttaccgac cggggcccct ccaaagccgt cttgaacgcg
     4081 tttgtagccg aggagaggac tctcgaggac cttgggggcc taacgaggtt ctatgagcct
     4141 tcaaagcagc ttctacgcac acctcagtca gcgaagtgta tcctctatat gagctagttc
     4201 gggatctttt tcggcgaact tcggagaatc ctccttccag gcagtaggct gaataggtag
     4261 gatgggcgcg agatggtgcg tgtctggatc attggcgtga tagctaccgc aggagtcggc
     4321 ttctactcca gcggctgcgg gcagcaggac gtgggcggat cttctcccga ctccgaacca
     4381 acgcatcaga ccaccacgtc gaggactctc gggatcgacc taggaagttg ccctaaagcc
     4441 gaacctgaag agctacctcc caatgcgctg gcaggtgcga ccgaggccgc gctggatcag
     4501 gtcccctcgg ttttcggtga ggtaaaagcc accgaaggtg cctacgctgt agctgcctat
     4561 cggatgaagg gtgggtccgg tccgttcggc ccgcggtccg ggatagttcg ggacacgtgc
     4621 cgcgagcgac cgcattacgc agggcgtctg ttagagcgta gcgtggaggt tgacctgata
     4681 ttcccgaggc tgcaatcgag cgcgagtctt tctcagcaca ccgtattcgt tgcacgcttc
     4741 cggggtgagt actgggtata cggagtaggg cgctagcgga ctacgccaac ttccgagaat
     4801 acctattcca cacactcggg tgaatagtga tcgtctaacc cccctcggga gcctgaacga
     4861 cgcagaagcg gttgccgtcg ggatcttcca gcacgataaa gtcgtcatcc aggctatatt
     4921 cttgcgggta ccgcctcgcc ccgatcccga ccagcctctc cacctcgcca tcttggtcgt
     4981 tcgtgtagag gtccaggtgc accctgctgg tcttcccgat tcgctccaaa ggttcaggaa
     5041 acctctccag agacacgttg ggacctctcc cctcgggatc gcgaaggacc acccaaccct
     5101 cctgcgcggg ctcccttggt acgtaatgga gcgcttcccg ccagaacgcc agcatcctgt
     5161 cgaactcgta gcaccggacg acgatcgaa
//
LOCUS       DASTKM010000167         4431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_749543, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000167 DASTKM010000000
VERSION     DASTKM010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749543"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..673)
                     /locus_tag="VFQ10_06940"
     CDS             complement(5..673)
                     /locus_tag="VFQ10_06940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET9965113.1"
                     /translation="MNTDDTRELLREIDVYVEDLFSPSDEALEAALRDSRRAGLPEIN
                     VSPNEGRLLQLLTEISGARRILEIGTLGGYSAIHFARALPDEGALISLEIDEHHAGVA
                     RNNIERAGLSEKVEIRVGDAHELLASLIEVVEGPFDVIFIDADKESYPDYLDASLRLA
                     RPGSLILGDNTIRGGTVLDPKDSTAQATREFNERIARDPRLSGIALPLIRERIDGLTI
                     ARVL"
     gene            complement(670..1431)
                     /gene="truA"
                     /locus_tag="VFQ10_06945"
     CDS             complement(670..1431)
                     /gene="truA"
                     /locus_tag="VFQ10_06945"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET9965114.1"
                     /translation="MKLAGLVEYEGTDFAGWAAQPERRTVEGVLSEALSTVLRQPVKM
                     SVAGRTDAGVHASGQVVSFRAETELRPALISYKTTAVLPKDVALRRCVVVPGDFDARR
                     DARSRTYEYRLVNDEIRSPLQRYHAGYAPQRLDLDLLSAAAELVEGTHDFRAFTPQKS
                     YHLRFERIVTSSRWTRHDDLLRYRITADSFLYSMVRALVGTMLEVAGGKRDLTEFDRL
                     LSGGKRSEAGPVVTSRGLTLVGVGYDDLDLWRSDR"
     gene            complement(1428..2216)
                     /locus_tag="VFQ10_06950"
     CDS             complement(1428..2216)
                     /locus_tag="VFQ10_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET9965115.1"
                     /translation="MTSRSLGQFYPVSSPVHDLDPRTKLLATAVFVVGLFLIDSMVGL
                     LSVAAALAALVGLSRIPAGTFLRLLRPVLLIVALTVLFQVFFSREGVTLFEWGFLEVH
                     SGGLRLGFFLALRILLLVSSAGLLTATTAPVALADGIEDLLSPLKRVRFPAHEVAMMM
                     TIALRFIPTLGEEAEKITRAQAARGADFSRGGPLRRARSFVPVLVPLTVGAFRRADEL
                     ADAMESRGYRGGEGRTRYRELRFRVGDALALAVTMLVLLVGVLL"
     gene            complement(2218..3033)
                     /locus_tag="VFQ10_06955"
     CDS             complement(2218..3033)
                     /locus_tag="VFQ10_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9965116.1"
                     /translation="MKLELRDVRHVYARGTPFEVEALGGVSLVVEPNEVLAIVGGTGS
                     GKSTLVQHMNLLLVPTSGEVLVEGVDATTLERSELRRRVGLVFQFPESALFAPTVEED
                     VSFAPRQFALGEEEVRERVLESLRALGAGELAPRSPFALSGGEKRRVAIAGVLAMRPE
                     VLVLDEPTAGLDPATREDLLALILGLRESGVSVVLVSHDLDEVAEVADRVCVLHEGRV
                     SAVGTAAEVFYGDPTQAPATVRVASLLRGSRPEIGDPVRFGETLDALRALQGA"
     gene            complement(3030..3890)
                     /locus_tag="VFQ10_06960"
     CDS             complement(3030..3890)
                     /locus_tag="VFQ10_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9965117.1"
                     /translation="MENPGEGTRPGGSFAVSCERVSFSYPGSGRPALSGVSLDLRQGE
                     YVGVVGPNGGGKSTLVRLLNGLLKPDSGRVLVSGRDPATEPFEVRKHLGVLFQNPENG
                     LVAPFVEDDVAFGLENLGVPREEMRERVARAIRTVGLEGFERREPHTLSGGEKQRVAL
                     AGLLVAEPEILVLDEPTSMLDSAGRMEVLEYLHALQSEKTVLHVTHHLEELTGADRIY
                     VLNGGELVADETPERFFSNAELLRENRLVLPVVPRLALGLGFPPAKLRTPEELAQAIL
                     ARTQSGTRPL"
     gene            complement(3899..4264)
                     /gene="rplQ"
                     /locus_tag="VFQ10_06965"
     CDS             complement(3899..4264)
                     /gene="rplQ"
                     /locus_tag="VFQ10_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET9965118.1"
                     /translation="MRHAKRGRKLGREAQHRNLMLGTMAGQVITHGRIKTTAPKAKEL
                     RGVVDKLINTAKRDDLASRRQAVKVLKDKTVVRRLFEDVAPELDDRNSGYTRILKLGP
                     RLGDGAETVYLELVNYTTE"
     gene            complement(4251..>4431)
                     /locus_tag="VFQ10_06970"
     CDS             complement(4251..>4431)
                     /locus_tag="VFQ10_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HET9965119.1"
                     /translation="VRSYNCLKREGVDTIGQLATMTEEELMNIRNLGMKSVDEIRSKL
                     LEYGYSLESEGNEAR"
BASE COUNT          757 a         1440 c         1440 g          794 t
ORIGIN      
        1 gtccttacag gacccgtgct attgtcaggc cgtctatgcg ctcccggatc aggggtaacg
       61 cgataccgga gagccttggg tctctggcta tccgctcgtt gaactcgcgc gtcgcctggg
      121 ccgttgagtc cttggggtcg agtacggtgc cgccccttat ggtgttgtcg ccgaggatta
      181 gagagcctgg tcgtgcgagg cgtagggacg catccaggta gtcagggtag ctctctttgt
      241 cagcgtctat gaagattacg tcgaaggggc cttcgacgac ttcgatgagc gaggcgagga
      301 gttcgtgggc gtcgccgacc cggatctcga ccttttcgga gaggccggcc ctttcgatgt
      361 tgttgcgggc gactccggcg tgatgctcgt ctatctccag ggagatcagg gctccctcgt
      421 caggcagggc gcgggcgaag tggatggcac tgtagccgcc aagggtgcct atctccagta
      481 tgcgccgcgc cccggatatc tcggtgagca gttgcagcag ccttccttcg ttgggcgaga
      541 cgtttatctc aggcagcccg gcccgccggg agtcccgcaa ggctgcttcg agggcctcgt
      601 ccgatgggga gaacaggtcc tcgacgtaga cgtcgatctc ccgcaaaagc tctctcgtat
      661 cgtctgtgtt catcggtcac tcctccatag atccaggtcg tcgtagccca ccccgaccag
      721 cgtgagcccg cggctggtta cgacggggcc tgcctcgctg cgttttcctc ccgagagcag
      781 ccggtcgaac tccgtcagat ctctctttcc gccagcaacc tccagcatgg tgccgaccag
      841 ggcgcggacc atactgtaca ggaacgagtc ggcggttatg cggtacctta gcaggtcgtc
      901 gtgcctggtc cagcgcgagc tggtaacgat tcgttcgaag cggaggtggt agctcttctg
      961 gggagtgaag gccctgaagt cgtgggtccc ttccaccagt tctgccgctg cagagaggag
     1021 atccaggtcg agtctctgcg gcgcgtagcc tgcgtggtag cgctgcaggg gggacctgat
     1081 ctcgtcgttc accaacctgt actcgtacgt ccgactcctc gcgtccctgc gggcgtcgaa
     1141 atcgcccgga acgacgacgc aacgacggag cgccacgtct ttgggcaata ccgccgtggt
     1201 cttgtaagag atcagggccg gcctgagctc cgtctccgct ctgaaggaga cgacctgccc
     1261 gctcgcgtgc acccctgcat cggtgcggcc tgcgacgctc atcttgaccg gctggcgcag
     1321 aacggtagag agcgcctcag agagtacacc ctcgaccgtc cgtcgttctg gttgggcggc
     1381 ccatcccgcg aagtcggtgc cttcgtactc gacgagccca gcgagtttca aagcagaacc
     1441 ccaaccagca ggacgagcat ggtgacggcc agtgcgagcg cgtcgcccac gcggaagcgc
     1501 agctcgcggt agcgggtgcg gccttcgccg cccctgtagc ccctgctctc catcgcgtcg
     1561 gcgagctcgt cggcgcggcg gaaagcgccg acggtcagcg gcacgagcac agggacgaag
     1621 gagcgcgccc tgcgaagcgg ccctccgcga gagaagtccg cgccgcgggc ggcctgggcc
     1681 ctggtgatct tctccgcctc ctccccgagc gtggggatga aacggagcgc tatggtcatc
     1741 atcatcgcga cctcgtgcgc tgggaaccgc accctcttca ggggagatag gaggtcctcg
     1801 atcccgtcag cgagcgccac cggggccgtc gtcgccgtaa gcaacccggc cgatgagaca
     1861 agcaagagta tccgcaaagc caggaagaaa cccaacctca acccgccgga atgaacctcc
     1921 aggaagcccc actcgaagag cgtgaccccc tcccgcgaga aaaagacctg gaacaagacc
     1981 gtcagggcga cgataagcaa cacgggccgc aagagccgca agaacgtgcc cgccggaatg
     2041 cggctcagac ccactagcgc cgcgagcgcc gcggcgacgg agaggagccc caccatcgag
     2101 tctatgagga agagcccgac gacgaacacg gccgtggcca ggagcttggt cctcgggtcg
     2161 agatcgtgca cgggcgagga cacgggatag aactggccga ggctccggct cgtcacctca
     2221 ggccccttgc agagccctca gcgcatcgag cgtctccccg aagcgcacgg ggtcgccgat
     2281 ctccggtctc gaccctctta gcaaggaggc cacccgtacg gtcgccgggg cctgtgtagg
     2341 atctccgtag aacacttcgg ccgcagtccc gacagcgctt accctacctt catgtagcac
     2401 gcacaccctg tcggcgactt cggcgacctc gtccaggtcg tgagagacga ggacgacgga
     2461 gactcctgat tcgcgcaggc ccaggatcag ggctagaagg tcctccctgg tggccggatc
     2521 caggccggct gtcggctcgt cgaggacgag gacctctggt ctcatcgcga gcaccccggc
     2581 gatggcgacc cgccgcttct cgccgccaga gagggcgaac ggtgaacgtg gcgcgagttc
     2641 ccccgccccc agcgcccgca gcgactccag cacccgctcc ctgacctcct cttcgccaag
     2701 agcgaactga cgcggggcga atgacacatc ctcctcgacg gtcggggcga agagcgccga
     2761 ctcggggaat tggaagacga ggccgaccct gcgtcgcagc tcgctccttt cgagcgtcgt
     2821 cgcgtccacg ccctcgacga gcacctcgcc actcgtcgga acgaggagca gattcatgtg
     2881 ctgtacgagc gtgctcttgc ccgagcccgt gccaccgacg atcgcgagca cctcgtttgg
     2941 ctccacgacg agcgacacgc ctcccagcgc ctcgacctcg aaaggggtgc cccgtgcgta
     3001 gacatgcctg acgtccctga gctctagctt cataggggcc tcgtcccgct ctgtgttctg
     3061 gccagtatgg cctgggccag ctcttcgggc gtacgtagct tcgccggcgg aaagccgagc
     3121 ccgagggcga gcctcggtac gacgggcagg accagcctgt tctcccgcag gagttcagcg
     3181 ttggagaaga atcgttctgg tgtttcgtca gccacgagct ctcccccgtt caggacgtag
     3241 atacggtctg cgcctgtgag ctcttcgagg tggtgggtca cgtgcaggac ggtcttctcg
     3301 gactgcaggg catggaggta ctccaggacc tccatgcgtc ccgcggagtc gagcatggag
     3361 gtgggttcgt cgaggacgag tatctcgggt tcggcgacca ggaggccggc cagggcgacg
     3421 cgttgcttct cgccgcccga aagagtatgg ggctcgcggc gctcgaagcc ttcgaggccg
     3481 acggttcgta tagccctggc gaccctctcg cgcatctcct cccgggggac gccgaggttc
     3541 tccaggccga aagcgacatc gtcctcgacg aaaggtgcga cgaggccgtt ctccgggttc
     3601 tggaagagga cgccgaggtg cttgcgaacc tcgaaaggtt ccgttgcagg atcgcggcca
     3661 gacaccagca cgcggcccga atcgggcttc aggaggccgt tcaagaggcg gaccagggtg
     3721 gacttgccac cgccgttcgg gcctaccacg ccgacgtatt ctccctggcg aaggtcgaga
     3781 ctcacccccg agagcgctgg ccgtccggag cctgggtagg agaaggatac tctctcgcaa
     3841 gacacggcga aggacccacc ggggcgggtc ccttcgcctg ggttctccaa gggttgggct
     3901 actccgtggt gtagttgacc agctccaggt agacggtctc ggcgccgtcg ccgagcctgg
     3961 ggcccagctt caggatacga gtgtagcccg agttacggtc gtcgagctcg ggggctacat
     4021 cctcaaagag gcggcggacg acggtcttgt ccttgaggac cttgaccgcc tggcgcctgg
     4081 aggcgaggtc gtcccttttg gccgtgttga taagtttgtc cacgacgccg cgaagctcct
     4141 tcgccttcgg ggccgtcgtc tttatgcggc cgtgtgtgat cacctgcccc gccatcgtgc
     4201 cgagcatgag gttgcggtgc tgtgcctcac ggccgagctt cctgcctcgt ttagcgtgcc
     4261 tcattaccct cgctctccag ggaataacca tactccagga gcttggagcg gatctcatcg
     4321 acgctcttca tgccgaggtt acggatgttc atgagctctt cctcggtcat ggtggcgagc
     4381 tggcctatgg tgtcgacccc ctcacgcttg aggcagttat aggagcggac c
//
LOCUS       DASTKM010000168        15017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1453913, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000168 DASTKM010000000
VERSION     DASTKM010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15017
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453913"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFQ10_06975"
     CDS             complement(<1..356)
                     /locus_tag="VFQ10_06975"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9965120.1"
                     /translation="MREPVFDEAVQLGIVVRDLEATVRSYVDDYGIGPWQFAQIDLGD
                     ANNYREYGQPVERSNRTAVATVGQVMWGLIEPLDEEGIYARFLAEKGEGIHHVAVATP
                     NLDETVARAERENNVIL"
     gene            complement(555..779)
                     /locus_tag="VFQ10_06980"
     CDS             complement(555..779)
                     /locus_tag="VFQ10_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HET9965121.1"
                     /translation="MDEKPDRDVEKVYSTSEFVAKLRRLADALETGERFEIQVAGQRV
                     YVPARAEFNLEHEREEDEEELEFQLKWTNR"
     gene            905..1728
                     /locus_tag="VFQ10_06985"
                     /pseudo
     CDS             905..1728
                     /locus_tag="VFQ10_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611155.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1786..2388)
                     /locus_tag="VFQ10_06990"
     CDS             complement(1786..2388)
                     /locus_tag="VFQ10_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9965122.1"
                     /translation="MFEESKSALGRSSGGGDEGVSVEAVPAKNVLEFPPEVGLKFVIT
                     KAAADTAGEFIEIEGTMRAHSGGPPIHVHPHQEETYRVTSGTADVFLDGRWHQVRAGE
                     SLTVPKGAPHTIKNEHDEDVTAMNWHRPAARFEEFSRTFHRLATSGRIKSLPPKDPSS
                     LVYAAMLFTEYEDTQVVVKPPTFVLRLLAFVGRRLGYRLD"
     gene            2525..3133
                     /locus_tag="VFQ10_06995"
     CDS             2525..3133
                     /locus_tag="VFQ10_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9965123.1"
                     /translation="MKRGKYNLKRRAERQEETRLRITRATLELHEILGPSKTTISAIA
                     ERAGVGRPTVYAHFPDELSLGKACSSLGLAENPPPDPGSWEEISDPEQRLRVALAELY
                     AYFRRRERMLANVLRDAEREWEAKPHVREIMEPLVEHWERMKGTLAAGWEVGKDHPQP
                     PLGAIGLALDFETWHTMVRKQGLTDEQALDLMVGMVRCAMQA"
     gene            complement(3231..5462)
                     /locus_tag="VFQ10_07000"
     CDS             complement(3231..5462)
                     /locus_tag="VFQ10_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9965124.1"
                     /translation="MVEVKRMLAMTGLLTLTGTGGCGKTRLALEVASELIGTYQDGVW
                     LVELAPLSEGALVPQVMASTLGVQEQQGLPPLQSLLDALGDKEMLLVLDNCEHLIDAA
                     ARLTVALLSSCPRLRVLATSREPLGVTGELGWLVPSLSAPDTPQSPTIEELGGYESVR
                     LFADRASSRHPGFELMSENAQAVAQVCARLEGIPLAIELAAARIGMLSVEQISDRLGH
                     SLKLLAGDGSADHRHQTLRAALEWSFELFGEPEQILFRRLSAFAGGFTLDAAESVGGR
                     VGIDEEDVLELLSMLVDKSLVVAKENWETGARYRLLEPTRQYAREKLRVSGEAEAVSR
                     RHAEFFLALAEEAEPELKGPRQVEWLDRLETEHDNLRAALSWALGRAIDLGPRMAGAL
                     CLFWYTRGYLSEGRRYLEAVARSDVIPATLRAKVLDGLGWIAEPQGDYERARAAYEES
                     LELYRSSNDKTGVANVLGDLGSLALDRGDYEEATSLLEESLTLHRELGSKEGVIGILD
                     SLGVLASAKGDREESIAFLSEALAVSRGIGNVRRNATSLSNLGITRLVHGDPEQAMVL
                     LEESLALFQEIGDSSNIAIGLMYSALAALIKGENERVEALGQESLDLLQKAEDKQHIA
                     DCLEILAGGAGAQGRTQRAARLWGAAEAVREDIGVPLQSEARKLLDPYLATVRSSLGE
                     VAWQLACAEGRAMMPEQAIEYSLSAEKPILPPPVARHLGEVSATLAPLTRREEEVAIL
                     GSV"
     gene            complement(5432..6142)
                     /locus_tag="VFQ10_07005"
     CDS             complement(5432..6142)
                     /locus_tag="VFQ10_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HET9965125.1"
                     /translation="MNLLWPDLGRKAASNNLRRVLHAARKVLDSATGSHYLASQDESL
                     MLCPGGQLWVDTEAFAESAVSARRSRDPSAYRAAIELYAGELLPDDRYEEWAENRRGQ
                     LRRLYFELLVELAGLHEERGEYGQAAEMLQRALSEEPTNEEAHAGLMRLYALSGRPGE
                     ALTQYERLRETLSGRLGTAPGATTRALRDKIAAGRFQSTQHTAPYHRTPASTTCPSPG
                     QTSSGVSERWSKSRGCSL"
     gene            6674..6838
                     /locus_tag="VFQ10_07010"
     CDS             6674..6838
                     /locus_tag="VFQ10_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965126.1"
                     /translation="MSLLFALVITLCAATVATLFVIMWRDGDLVAAAALAFALLPVLI
                     LTIIAWRAAL"
     gene            complement(6938..7417)
                     /locus_tag="VFQ10_07015"
     CDS             complement(6938..7417)
                     /locus_tag="VFQ10_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9965127.1"
                     /translation="MSIFPTRILLATDGSREAQLAASTAADLANTTNSELHVLTVAPG
                     NPDPVYHIHEGGLRYETYQQALEAVREDAQKVLDEQVQKLEEAGGSVKEAHLRLGERR
                     DQAIVHLAEELGAGLIVMGSRGLGGVRRALMGSVSASVVHHAHCPVMVVRPEKERAE"
     gene            7631..8419
                     /locus_tag="VFQ10_07020"
     CDS             7631..8419
                     /locus_tag="VFQ10_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9965128.1"
                     /translation="MPELPELEVFKQYVDSTSLHQDIEMVEVKNGKILGGVTAGGLKR
                     RLEGRKFESTRRHGKYLFVELDEGPWLLLHFGMTGGLNYYEDAVEEPTHARVLISFRN
                     GYHLAFDDQRLFGKVDLIEDPDDYVEEHMLGPDPLDLEFPVFRERLEGRRGEIKATLM
                     NQRVFAGIGNIYSDEILFQVRLHPKTSVGRLDESSLHGLHEQTGRVLRAAIERGADPG
                     GLPAWFLLPHRQEGADCPRGNGTVLKTKAAGRTAYYCPTCQPRV"
     gene            complement(8416..8898)
                     /locus_tag="VFQ10_07025"
     CDS             complement(8416..8898)
                     /locus_tag="VFQ10_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET9965129.1"
                     /translation="MSTENERLLDTTGWRILEELQGNARLTHKELGRRVNLSAPAAAE
                     RMRRMEEAGIITGYRAQVDAEKVGLPLAAFVRIEVPGANHKKVEALVVELPEVLECHR
                     ATGTDCYVMKVVLASMEHLQKLLDKLVLWGSPVTSIVISSPVPGRGIQRGPIPLEDGG
                     "
     gene            9024..9905
                     /locus_tag="VFQ10_07030"
     CDS             9024..9905
                     /locus_tag="VFQ10_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9965130.1"
                     /translation="MDTTEGQASDWFAMPALASAGVLWGVSFLFGKWALEEMGPAHVT
                     LLRFSLASAALLPYALLKGVWPWWRDLPLFLLVGFLTVPATFLIQFWGLSLTGATVAA
                     LIVGCGPPIVAFFASLFLGERLGKRGWSAIGASTLGVALAVARPGVSNHWLGDALVLL
                     SLLAVVGWVLLGKRLGDRYPAVPATAWILTFGTVTLAPVALLWEGVPRLDLTLLCWAS
                     VLVLGLGCSAATYALWNWGVARVPASRAGIFLNLEPLVGALLGILILGEAWGPATIVG
                     GALIVGAALVVSHRSSV"
     gene            complement(10139..10357)
                     /locus_tag="VFQ10_07035"
     CDS             complement(10139..10357)
                     /locus_tag="VFQ10_07035"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HET9965131.1"
                     /translation="MGLGNLGDLSNLDLGQLQQYLPNLNFPASKDEVISEVQNNGAPQ
                     DVIDQIRNSSTDTFNNAEEVLETVRGNR"
     gene            10549..10782
                     /locus_tag="VFQ10_07040"
     CDS             10549..10782
                     /locus_tag="VFQ10_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965132.1"
                     /translation="MTTDKDKGIVGLASDRLRETSGGLRGEGPWHSAKSEVYHKNRNR
                     QTGNSIAPENIEQGTGGKPLCEECERLNSLGVQ"
     gene            11025..11468
                     /locus_tag="VFQ10_07045"
     CDS             11025..11468
                     /locus_tag="VFQ10_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965133.1"
                     /translation="MKMIEAEGARVERPRTAVVEIRKHLAGLYRGFVWWASLYGDVDD
                     HYEKERRELVVGLHDELSNQYLPRSVWLTEGSRKKVESFLRMSEELCSEFSAEIEARG
                     YPRVRHSMERRVIKKLRPLKTEAESGLETELGEPRSQGWRKRLRK"
     gene            complement(11687..12664)
                     /locus_tag="VFQ10_07050"
     CDS             complement(11687..12664)
                     /locus_tag="VFQ10_07050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9965134.1"
                     /translation="MSEEKKGGTLARADAVVVGCGVIGLTAAICLREAGLDSGIVAAR
                     LPHDTTSSVAAAIWYPYKAYPEDNVLSWGSRTFEIFEELSGTPESGVLMREGVEIWRQ
                     PVPDPWWSGAVPHIRRCNDDELPPGCRDGHAFVAPVVEMPIYLEYLLDRFSAAGGSVQ
                     RRTLSSLDEVDARVVVNCVGLGARDLVDDASMEPIRGQIVRMRNPGLKRFVLDEENPE
                     GVAYIVPRSGDCILGGTAEEGEWDLEPDPETAAGILRRCTRLEPRLRGVEVLEHKVGL
                     RPGRPEIRLEREDGVIPRIHNYGHGGSGITLSWGCAEETLRLVQEALGR"
     gene            12773..14413
                     /locus_tag="VFQ10_07055"
     CDS             12773..14413
                     /locus_tag="VFQ10_07055"
                     /inference="COORDINATES: protein motif:HMM:NF006052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9965135.1"
                     /translation="MADRGMKAAGYVAAEVMARAGVRRLYTVPGESFLEILDAAEEHP
                     DLSLVSTRHESGASFMAEAEGKLTGRPGVAMATRGVGASNLAIGVHTARQDSTPMLVM
                     LGQVETDFLGREAFQEVDLSAFYAPITKWAATVHRPDRLAEFVARGLRIATSGRPGPV
                     MLALPADVLGEAVQDNPEAHVPPTPRSALAPEDIRTVAGRLAAARRPVVIAGGGAQGA
                     RESLISFSEAWGAGVYAAFRRQDVFPNEHPNYLGHLTLGTPQETLQGLERADLVLVVG
                     CRLSEVTTQSYSLPRGDQAVIQIDIDPNEVGAAVPTEIGMVSDAGTALAQLAEQAPPS
                     PPARNWTQGHRAYLDSSTLPPDRAREGIDPTRVVGAMREALPEDALLTNDAGNFSVFI
                     HRYWRYNHSRTQLAPTNGAMGYGVPAAVAAKLAAPDRTVVACCGDGGFLMTGQEIETA
                     VRYGAAITVVVFRNRLHGTIAMHQKQTLGRTAGVEIGEVDLAGYARSLGAEGHTVRDP
                     DGLAPAMEAALASDTVTLLDVVTDPDIISPSTTLSESH"
     gene            14764..14961
                     /locus_tag="VFQ10_07060"
     CDS             14764..14961
                     /locus_tag="VFQ10_07060"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HET9965136.1"
                     /translation="MRKRLPAQVGKYLEGVEFPARKEELLGMLERNGVPGTIVGQLRK
                     RLPEGEYRGPQDVVSALRRGR"
BASE COUNT         2723 a         4764 c         4621 g         2909 t
ORIGIN      
        1 aggatcacgt tgttctcacg ctcggcccgc gcaacagtct catcgaggtt cggtgtggcc
       61 acggcgacgt gatgtatgcc ctcacctttc tcggcaagga aacgcgcata gatgccttcc
      121 tcgtcgagtg gctcgataag cccccacatc acctggccga ccgtggcgac ggcggtgcgg
      181 ttggagcgct cgacgggctg accgtactcg cggtagttat tcgcgtcacc gaggtcgatc
      241 tgagcgaact gccacgggcc gatcccgtag tcgtccacgt agctccgtac cgtcgcctcc
      301 agatcgcgca cgacgatccc cagttgcacc gcttcgtcga agacgggctc tcgcatcgtt
      361 cccctcccct aagtcgatga ggttatctta ctgaggcgat cttaccggaa cgcggacccc
      421 cgaccaccgg taaacttcgc agaactcccc ttccacgcac tcgggtgaat aagggcaaga
      481 agaggaaggg ccgcacccag agttctagca gggccctcca gaatgcgcag tactcagcat
      541 gaaagcggag gaggtcaccg gttggtccac ttcagctgga actcgagctc ctcttcgtcc
      601 tcctcccgct cgtgctcgag gttgaactcc gctcgggcgg gcacgtagac tctctgcccg
      661 gcaacctgga tctcgaagcg ctcgcctgtc tccagagcgt cggccagcct gcgcagcttc
      721 gctacaaact cggaggtgga atagaccttc tcgacgtccc tgtctggctt ttcgtccatc
      781 aatgctccct gggttcgctg aactctcgac ctttcggtat cctatcgggt ccgaggggca
      841 gagccaagtc acctaggctc tgtctgagaa cgacaaagaa taattctgct gctacgctgc
      901 tcccatggta ggacgatacg aactctacga gtggcaatgg caactcattc gcgatctttt
      961 tcctcgcccg aaggccaccg gccgtaagcg gcgtgaccct aggcagatgc tcaacgcggt
     1021 tttgtggata ttgtgcagcg gtgcttcttg gcgagacctt ccggagcgtt acggcccctg
     1081 gcaaagcgcg taccactggt accgcacatg gaccaaagat ggctccttcg accgcatctt
     1141 ggagcggctc cagatccggc tgaacgcgga agggctgatg gatctcggca cctggtttgc
     1201 cgactccacg aacgttaggg cctcccgggc cgcggccggg gcgaaaaagg gggccgtgaa
     1261 gccttaggcc gctcccgcgg cggcttctcg acgaagctgc acctggtttg cgacggtctg
     1321 ggcaacccct tagcagcgct cctcactccg gggcagcagc acgagagcac cgtctgccag
     1381 gaactgctgg caagcgtgag ggtacggacc agcgcgggag gcggcagacc gcgcacccgc
     1441 ccgaagctgg tggtggcgga taggggttac gatacgggcg gcttccgaag atacctgcgt
     1501 cgtcgcggca tccgctgtgt gatccccgaa aagcgcgtcc ccgcgggcaa gcggcgcagg
     1561 cggcgcgggc gtcctcccgg cttctgcggg gccacctacc gacggcgcaa tgtgatcgag
     1621 cgtctggtgg gatggctcaa ggagcaccgg cggatcgcca cgcgcttcga gaagctgggc
     1681 agcaattata tggcgatggt gaagctgtcg ttcgtgcggc gttacttccg cgcgttggag
     1741 caatccttac gcagtacgtg gccctacggc cgcaagcccg atgcctcagt cgagtctgta
     1801 gcccaaccgc ctgcccacga aggccagcaa cctcaggacg aacgtcggcg gcttgaccac
     1861 gacctgggtg tcctcgtact cggtgaaaag catcgccgcg tagacgagcg acgatggatc
     1921 cttcggggga aggctcttta tcctgccgga tgtcgccaag cggtggaagg tcctcgaaaa
     1981 ttcctcgaac ctcgcggcgg gccggtgcca gttcatggcc gtgacgtcct cgtcgtgctc
     2041 gttcttgatg gtgtgcggcg ctcctttggg gacggtgagc gattcgccgg cgcgaacctg
     2101 gtgccagcga ccgtccagga acacgtccgc cgtgccggac gtcacccggt aggtctcctc
     2161 ctgatgagga tggacgtgaa tcggcggacc accgctgtgg gcccgcatgg tgccctctat
     2221 ctcgatgaac tcgccggcgg tatcggcggc ggccttcgtg atgacgaact tcagcccgac
     2281 ctcgggcggg aactcgagca cgttcttcgc tgggaccgcc tcgacgctca ccccttcgtc
     2341 gcctcctccc gaagaccgac cgagcgcgga tttgctttct tcgaacatgg accttcgctc
     2401 ctctccctcg cctctctgca atcactttac gatagtgtaa aatgattgag gacatgatgc
     2461 cccctgcgtg aggggttgtc aaggggaagc gggccgtccg tccggattcg cttagaattg
     2521 gatcgtgaag cgcgggaagt acaacctgaa gaggcgcgcc gagcgccagg aggagacccg
     2581 gctgcggatc accagggcga ccctcgagct gcacgagatc ctcgggccct cgaagaccac
     2641 cataagcgcc atagccgaga gggcgggggt gggcaggccc accgtctacg cccacttccc
     2701 cgacgagctc tccttgggca aggcttgctc gtcgctcggc ctggccgaga atccccctcc
     2761 cgaccccggg tcttgggagg agatctccga ccccgagcag cggttgaggg tggctttggc
     2821 cgaactctac gcctactttc gtcgcaggga gcggatgctg gcgaacgtgc tgcgagatgc
     2881 cgagcgggag tgggaggcga agccccacgt gcgcgagata atggagccct tggtcgaaca
     2941 ctgggaacgc atgaagggaa ccctggccgc gggatgggag gttggcaaag atcacccgca
     3001 gccgccgctc ggtgccatag ggcttgcgct ggacttcgaa acctggcaca cgatggtacg
     3061 caagcagggc ctaaccgacg agcaggcgct ggatctgatg gtgggtatgg tgagatgcgc
     3121 catgcaagcc tgaaggagat ggcatcgcat ccgtgcgccc ggacttagcg agagctcggg
     3181 accacgtcgg gtgcttctga cctttgggaa cgctccgcaa gttaaccgtt tcagacagag
     3241 cctagtatcg ctacctcctc ctcgcgacgg gtaagagggg caagcgtggc agagacctca
     3301 cccaagtgac gggcgaccgg tggaggcaag ataggtttct ctgcggagag ggagtactcg
     3361 atagcctgct caggcatcat tgctcggccc tctgcacatg ccaattgcca tgccacctca
     3421 cccaggctgg aacgtaccgt ggctagatat ggatctagta gcttgcgagc ttcggactgc
     3481 aagggcacac cgatgtcttc acgcacggcc tccgctgccc cccacagccg cgccgctcgt
     3541 tgcgtcctgc cttgcgctcc ggctccgcct gccaatatct ccaggcagtc ggctatgtgc
     3601 tgcttatctt cggctttctg gagcagatcc aagctctctt ggcccagcgc ctctactcgt
     3661 tcattttcac ctttgatcaa cgcggcgagg gctgaataca tgaggccaat ggcaatgttc
     3721 gagctatcac caatttcttg gaatagcgcc aaactttctt cgagcaacac catcgcctgt
     3781 tcgggatcgc catgcaccaa cctcgtgatc cctaggttac tcagagaggt agcgttcctc
     3841 cgtacgtttc caatcccccg gctcaccgct aaggcttcac ttaggaacgc tatagactcc
     3901 tctcgatctc ctttggctga tgccaagacc ccgaggctat cgagtatgcc aatgacccct
     3961 tctttactgc ctaattctcg gtgtaacgtc aagctctctt ccagcagcga agttgcctcc
     4021 tcgtagtctc ctcgatctag cgccagagat cccaaatcgc cgagcacgtt cgctacgccc
     4081 gttttgtcat tggaacttcg gtacaactcc aggctctctt cgtatgctgc tcgtgctcgt
     4141 tcgtaatcgc cttgcggctc cgctatccat cccaacccat caagcacctt ggcgcgcagg
     4201 gtcgctggta ttacgtcgct cctcgcaacc gcctccaggt accttctccc ctcgctcaaa
     4261 taacctcgcg tataccagaa aaggcacaag gcacccgcca tcctaggtcc cagatcaatc
     4321 gcccgcccga gcgcccaaga gagcgcagcc ctgaggttgt cgtgctcagt ctccaaacga
     4381 tctagccact ccacctgccg cggtcccttg agctccggct cggcctcctc cgccaaggcg
     4441 aggaagaact cggcgtgccg acggctaact gcctcagctt ccccgctcac ccttagcttc
     4501 tccctggcgt actgccttgt gggctcgagc agtctgtagc gtgcccccgt ctcccaattc
     4561 tctttagcca ctaccagcga cttatccacc agcatggaga gcaactccaa gacgtcttct
     4621 tcgtcgatac ctactcgccc tccgacgctc tcggctgcat ctagggtgaa gccgccagcg
     4681 aacgcggaga gcctcctgaa cagtatttgc tcgggctcgc caaacaactc gaagctccac
     4741 tccagcgccg ccctcaaggt ctggtggcgg tggtccgctg acccgtcgcc cgcaagtagt
     4801 ttgagggaat gcccgagcct gtcggagatc tgctcgacag agagcatccc gatcctcgct
     4861 gcggcgagct ctatggcgag cggaatgccc tccagcctcg cgcacacctg tgccaccgcc
     4921 tgagcgttct cggacatcag ttcaaagccc gggtgcctac tagaggcacg atcggcaaac
     4981 agccgcacgg actcgtagcc tccgagctcc tctatggtag gggactgtgg cgtgtcgggc
     5041 gccgagagtg agggcacaag ccagcccagc tcgcccgtca cgcctagagg ctccctgctg
     5101 gtggccagca cccgcaaacg cggacaagag ctgagcaggg ccactgtcag gcgcgctgcg
     5161 gcgtcgatca ggtgctcgca attatccagt acgaggagca tctctttgtc gcccagggca
     5221 tcgagtaggc tctgaagggg gggaaggccc tgttgctctt gcacccccaa agtgctggcc
     5281 atcacctggg gtaccagcgc cccctcggac aacggcgcca gctccacgag ccacaccccg
     5341 tcctggtatg ttccaatcag ttcactcgcc acctcaaggg cgagccgcgt cttgccacag
     5401 ccgccggtac cggtgagggt caacagcccc gtcatagcga gcatcctctt gacttcgacc
     5461 atctctcgct cacgcccgac gaagtttgtc ctgggctcgg gcaggttgtg cttgccggcg
     5521 tacgatggta gggagcggtg tgctgggtgg actggaatcg gccggctgct atcttgtcac
     5581 gcaatgctcg ggtcgtggca ccgggcgccg taccgagccg cccggagagg gtctccctga
     5641 gccgttcgta ttgcgtgagc gcctctcctg gcctcccgga gagggcgtat agacgcatca
     5701 ggccagcgtg cgcttcctcg ttggtgggtt cttctgataa ggctctctgc aacatctcag
     5761 cagcctgccc gtactccccg cgttcttcat gcagtccagc cagctcgacc aacagctcga
     5821 agtacagtcg ccgcagttgc ccccgcctgt tctccgccca ctcctcgtag cgatcatcgg
     5881 gcaggagttc ccctgcgtac agttcgatgg ccgcccggta ggcagagggg tctcgggagc
     5941 gtcgggcgct gaccgcggac tccgcgaagg cctcggtgtc cacccacaac tgtcctcccg
     6001 gacacaacat cagcgactcg tcctgggatg ccaggtagtg ggagcccgtc gccgagtcca
     6061 ggactttgcg ggcggcgtgg aggacccggc ggaggttgtt cgacgcggcc tttcgcccaa
     6121 gatccggcca cagcaggttc atcacctgct ctcggtgaat gcgatgaccc ggcgcgatgg
     6181 ctagaagttt gaccaggctg gccgccttcc ggagccgcca ctgatcttgt tctatggtcc
     6241 gagacccaac cgaaacccag aagcccccga gcaaccagac gcgcactgcc tcaggtgccg
     6301 agtcctggcg gcctcgcgaa ggacgatcaa agcgctctgc tctccccgta gacatctccc
     6361 atccagcagt ccggccgctt ctccctacag agcgagaagc ccggagcttt gcaccctcct
     6421 tacaatgggg gcgctcctat cgtagggttt gctacactcc tcaccttcag tccgcaccat
     6481 acatgaagta gaggagatct acatcactca aatgggtgat ttcctgggta tgaaggcttt
     6541 ccgagagtct gatgaatcga gttcaggctc gcgcggagta ccttctcttc cattatcgga
     6601 atgtgctacg gtggcgaaca cgcatggtcg cgctgctcag caacagcgaa atcgagggca
     6661 tgaagaggtt ggcatgagcc tcctgttcgc cctggtcatc accctctgcg ccgcaactgt
     6721 cgcgactctc ttcgtaatca tgtggcgcga cggagatctc gtggcggcag cggcgttggc
     6781 tttcgcacta ctgcccgtgc tcatccttac gataatagcc tggcgagcgg ccctgtgagg
     6841 gaacccaaca agcgaacttc gcggaccgca ccttctttct aggaggttcg ataattgcga
     6901 gcaagaggca ccggtgtccc caagtgggct ctcgacctta ctctgcccgt tccttctccg
     6961 ggcgcacgac catcacaggg cagtgagcgt ggtggacgac ggacgctgag acgcttccca
     7021 tcagcgcccg ccggacgccg ccaagtcctc ggctccccat gactatgagc ccggcgccca
     7081 gctcctcagc caagtggacg attgcctgat cgcggcgttc tccgagccta aggtgggctt
     7141 ctttcacact ccctcccgcc tcttcgagct tttgcacctg ctcgtcgagc accttctgag
     7201 cgtcttccct taccgcttcg agcgcttgtt gataggtttc gtaacgcaag ccgccttcgt
     7261 gtatgtggta aacgggatca gggttaccgg gcgctacggt gaggacgtgc aactccgagt
     7321 ttgtggtatt ggcgaggtcg gcggcggtgc tggcggccag ctgtgcctcc ctggagccgt
     7381 cggtggctag caggatcctg gttgggaaga tactcattgg tcgtctcctt gctcgggaca
     7441 gtcctatgcc ttgataatgc catgagccgc agcgcgatgc acccgcatgg ggacctgaga
     7501 tgtcggtacg cggctcgcca cggacgatcg agagctcgaa aatccgtgat atggtcgtgg
     7561 atcggggcgc tatggtccac gtcgtcgaag gtaaggaagt agttgcgaga tagcgtaagg
     7621 aggggtcgtc gtgcccgagc ttcccgagct agaggtcttc aaacaatacg ttgactccac
     7681 gtccctgcac caggacatcg agatggtcga ggtgaagaac gggaagatcc tcggcggtgt
     7741 taccgccggc gggttgaagc gcaggctcga gggcaggaag ttcgagtcca cgcggcgtca
     7801 cggcaagtac ctgttcgtgg aactggacga aggaccatgg ctgctgttgc acttcgggat
     7861 gaccggcggc ctgaactact acgaagatgc ggtcgaagag cccacccacg ccagggtact
     7921 gatctccttc agaaacggtt accatctggc gttcgacgac cagcggttgt tcggcaaggt
     7981 ggacctgatc gaggaccctg acgactatgt cgaagagcac atgcttgggc cggacccact
     8041 ggacttagag ttccctgttt tccgggagag gctcgaaggg aggaggggtg agatcaaggc
     8101 gacgcttatg aatcagcggg ttttcgccgg gatcggcaac atctattctg acgagattct
     8161 cttccaggtc cgcctccatc ccaaaacgag cgtcggacga ctggatgaaa gctccctcca
     8221 cggtctccac gagcaaaccg ggcgcgtgct gcgcgcagcc atcgaacgcg gggcagatcc
     8281 cggaggcttg cccgcatggt tcttgctccc ccaccggcag gagggagcgg actgtccccg
     8341 gggcaacgga acggtcctga agacgaaggc ggcgggccgc accgcgtact actgtccaac
     8401 gtgccagcca agagtctaac cgccatcctc gagggggatc ggcccacgct ggatcccgcg
     8461 ccccggtacc ggagaagaga tgacgatgga ggttaccggg ctgccccaca gcaccagctt
     8521 gtcgagtagc ttctgcaggt gctccatgga tgccaggacg accttcatca cgtagcagtc
     8581 tgtgcccgtg gcgcggtggc actcgagcac ctcgggtaac tcgactacca gagcctcgac
     8641 cttcttgtgg ttcgcgccgg gcacctcgat cctcacaaaa gccgcgagcg gtaggcccac
     8701 tttctcagca tctacctggg cgcgatagcc ggtgatgatg cccgcttcct ccatcctcct
     8761 catcctctcg gccgccgccg gcgccgagag gttgacccga cggcccaact ctttgtgggt
     8821 gagcctggcg ttgccctgca actcctccag gatgcgccac cctgtagtgt ccagtagcct
     8881 ttcgttttct gtgctcatcc tcggaaacac tttcgttagt gggcatagtt tcgcgaaaca
     8941 cattctaccg gccattccga aaacgtggtt ctcggtgtgt actaggagcg tacccccatg
     9001 tcgggttgac gaggaggtta catttggaca ctacggaagg ccaagcaagc gattggttcg
     9061 ccatgccagc actggcatcg gcgggcgttc tgtggggagt gagcttcctt ttcggcaagt
     9121 gggccctgga ggagatgggc ccggcccacg tcacgctcct gcgcttctcc ctcgcctcgg
     9181 cggccttgct gccctacgcg ttgctcaaag gggtgtggcc ctggtggcga gacctgcctc
     9241 tgttcttgct ggtggggttc ctgactgtgc cggccacctt cttgatacag ttctgggggc
     9301 tctcactaac cggcgctact gtagcggccc tgatcgtggg ctgcggcccg ccgatagtcg
     9361 cgttcttcgc gagtctcttc ctcggggaga ggctcggcaa gagggggtgg tcggcgatcg
     9421 gggcgtcgac cttgggtgtg gcgctcgccg tcgccagacc aggcgtctcc aaccactggc
     9481 tgggcgacgc gctagtcttg ctgtcgctcc tggccgtggt cggatgggtg ctgctgggca
     9541 agcgcctggg cgataggtac ccggccgtgc ccgccaccgc ctggatcctg actttcggca
     9601 cggtgaccct cgccccggtg gccctactgt gggagggtgt gccccggctc gatctgacgc
     9661 tgctctgctg ggcgtcggtg ctggtcttgg gcctggggtg ttcggcggcc acttacgcac
     9721 tgtggaactg gggcgtggcc cgcgtgcccg cctcacgcgc cgggatcttc ttgaacctcg
     9781 agccgctggt gggggcgttg ctagggatcc tgatccttgg ggaggcgtgg ggaccagcta
     9841 caatcgttgg cggcgcgctt atcgtcggcg ccgccttggt cgtctcgcac cgcagtagcg
     9901 tgtgaatggc ctgttgaagt aggatagacg ctgtgcggac tgttgtcaca atccttttcg
     9961 gacctatgga ccatcaaggt ggcacacatc gcctactctg gtcctcagct ctgctttcag
    10021 acaaatcctg acgcctgaac cctctttgag cggtgctcct ggagatctat tcgagaagag
    10081 atctggaccc agggaaggcc ctgggtccag gtcagaggta gagattctcg cctgttctct
    10141 acctgttccc tcgcaccgtc tccaatacct cttcggcgtt gttgaaggtg tcggtgctcg
    10201 agttccttat ctggtcgatc acatcttgtg gcgccccgtt attctggacc tccgatatga
    10261 cctcgtcctt gctggccggg aagttaaggt tcggcaggta ttgctggagt tgtcccaggt
    10321 cgagattact caagtcgccc agattgccaa gacccatggt cttgttcctc ccatctcact
    10381 ttctatcaac gtcaagtgcg ttcttaccca cattgccctc tctgccaaac aaacgcctcc
    10441 accgtccgag agacccttgc gcgcctgcca gacctcgaac ccgaatgttt gatctccgtg
    10501 tcatttgctt gcatatcagg caatcgcaag caatgtgagg aggaacacat gaccaccgac
    10561 aaggataagg ggatcgtggg tttagcttcg gaccgcctca gggagaccag cggtggcttg
    10621 aggggcgagg gcccatggca ctcggccaaa tcggaggtct accacaagaa ccgcaatcgc
    10681 cagaccggca acagcatcgc gcccgagaac atcgaacagg ggacaggcgg caagccgctc
    10741 tgcgaggagt gcgaacgact caacagcttg ggggtccagt aggcaacctc accaacctgt
    10801 aacagcaacg caatggctgc aaacgcgccg cccaggagcg cggcgcgttc tatgcacgct
    10861 cgtaactagc cgggccatgc agcgctccgc aaggaagccg atcgtcgcaa aagtcactct
    10921 tcccggagct gaacagaggc cccatgttgt gctgtgtttc gtgttagccc acaagcgagt
    10981 gtggtgcaat gtgagacgga attgtatggt ggaggggctc ggaattgaag atgatagagg
    11041 ccgagggggc acgggtcgag cgacctcgaa cggcggtcgt ggagatccgg aagcatctcg
    11101 ccggactcta cagaggtttc gtgtggtggg cttctctgta tggggatgtg gacgaccact
    11161 acgagaaaga gcggagggag ctggtcgtcg ggcttcacga tgagctatcc aaccagtatc
    11221 taccgcggtc agtgtggctc acggaaggta gccgcaagaa ggtcgagagc ttcctcagga
    11281 tgtcggaaga gctctgctcc gagttctccg cggagataga ggcgcggggc tacccgcggg
    11341 tcagacacag catggagagg cgagtgatca agaagctgag gccgctcaaa acggaggccg
    11401 agtccggact cgaaaccgag cttggggagc cccgcagtca ggggtggcgc aagcgcctac
    11461 gcaagtagta agcacgctcc gccttcgcta ggcagcactt cagcacggcg gctgcgcctt
    11521 gtcagcacgc tcaggctgac gccttcgctt ctcagcatgt cgggttttgc ggtaggagat
    11581 gattctcaaa gagtacctac tcctgtggca ttcatctcac cacccttgac attgctgagt
    11641 ggctgacaag cgaaagccga aggctggagc gtgctgtaat gctactctaa cggccgagag
    11701 cttcttgcac gagcctgagt gtctcttcgg cgcagcccca ggagagggtg atgcctgatc
    11761 ccccgtggcc gtagttgtgg atgcggggaa ttacgccgtc ctctcgttcc aggcgtatct
    11821 cgggccggcc cggcctgagt cctactttgt gctccagcac ctcgacgccc ctcagtcgcg
    11881 gctccagccg cgtgcagcgg cgtaggatac cggctgcggt ctccgggtca ggttcgaggt
    11941 cccactcgcc ctcctcggcc gtaccgccaa ggatgcagtc acccgagcgg ggaacgatgt
    12001 acgcaacacc ctccggattc tcttcgtcca gcacgaaacg ctttaggcca gggttgcgca
    12061 tacgcacgat ctggccccgg atcggctcca tagaagcgtc gtctaccaga tccctggctc
    12121 ctagtccgac gcagttgacg accacgcgcg catctacctc gtcgagcgag gagagggtgc
    12181 gcctctgcac gcttcccccg gcggcgctga agcggtctag caggtactcg agatagatgg
    12241 gcatctcgac cacgggcgcg acgaaagcgt ggccgtccct gcagccgggc ggtagttcgt
    12301 catcgttaca tcgacggatg tggggcacgg caccggacca ccacgggtca gggaccggtt
    12361 ggcgccagat ctccacgccc tcgcgcatga ggacgccgct ctcgggagta cctgagagct
    12421 cctcgaagat ttcgaaggtc ctgctgcccc aggagagcac gttgtcttca ggataggctt
    12481 tgtacgggta ccagatcgcg gccgcgacgc tcgacgttgt gtcgtggggc aacctggcag
    12541 ccacgatccc ggagtcgagc ccggcctccc gcaaacagat ggcagccgtc agcccgatca
    12601 caccgcatcc cacaaccaca gcatccgcgc gggccagagt tcctcctttt ttctcctcgg
    12661 acacactata tgtcctgccg acgccttccg cgacctcttc ggagggcgag ggtgtacgat
    12721 tacgggttgt cggagatgtg gtcccgatgg gccaggatcg ttcggaggac ggatggcgga
    12781 tagaggtatg aaagcggccg gatacgtcgc ggccgaggtt atggcgcggg ccggtgtgag
    12841 gcgcctgtac acggtgcccg gcgagagctt cctggagatc ctggacgccg ccgaggagca
    12901 ccccgacctc tccctcgtct cgacgcgcca cgagtctggc gcctccttta tggccgaggc
    12961 ggaaggcaag ctgacgggga ggccgggggt ggccatggcc acgaggggag ttggcgcatc
    13021 caacctcgcc ataggggtac acacagcgag gcaggactcg acgccgatgc tcgttatgct
    13081 cgggcaggtc gagacggact tcctcgggag agaagccttt caggaggtgg atctcagcgc
    13141 cttctacgcc ccgatcacga agtgggcagc aacggtgcat cgccccgacc gcctcgccga
    13201 gttcgtcgcc agaggactga ggatcgccac ttcaggccgt ccaggccccg tgatgctcgc
    13261 gctgccagcg gacgtgctcg gtgaggcggt ccaggataac ccagaggccc acgtgccgcc
    13321 tacgccccgc tctgcccttg cgccggagga tatccggacc gtcgcaggaa ggctcgccgc
    13381 cgctcgccgt cctgtcgtga tcgctggcgg cggggcgcaa ggcgcccgag agtctttgat
    13441 ctccttttca gaggcttggg gtgccggcgt ctacgcggcc ttccgcaggc aggacgtgtt
    13501 cccaaacgag catccgaact acctgggaca cctcacgctc ggtacgccac aggagaccct
    13561 gcaggggctc gagagggcgg atctcgtact ggtggtcggc tgccggctta gcgaggttac
    13621 cacccagagc tactcgctcc cgcgcgggga ccaggccgtg atccagatag acatagaccc
    13681 gaacgaagtc ggcgcggcgg tccccaccga aatcgggatg gtctcggacg cgggtacggc
    13741 cctggcccag ttggccgaac aggcgccccc ctcacccccg gcgcggaatt ggacccaggg
    13801 ccacagggcc tacctcgact ccagcacgct acccccggat cgtgccaggg aaggcatcga
    13861 ccccacgcgt gtcgtcggcg ccatgcgcga agccctgcct gaggacgctc tattgaccaa
    13921 cgacgcgggg aacttctcgg tcttcatcca ccgttactgg cgctacaacc actctcgaac
    13981 tcagctggcc cccacgaatg gggcgatggg ctacggtgtt cctgcagctg tggcggcgaa
    14041 gctcgctgcc cctgaccgga ccgtcgtggc ctgctgcggc gacggcgggt tcctgatgac
    14101 gggccaggag atcgagacgg cggtgaggta cggcgccgcc atcacagtag tcgtcttccg
    14161 caatcgacta cacggcacca tagcgatgca ccagaagcag acgctcggcc gcacggccgg
    14221 cgtcgagata ggagaggtgg acctcgccgg ttacgccagg agcctcggag ccgaaggcca
    14281 caccgtgcgc gacccagacg gactggcgcc cgcgatggag gccgcgctgg cctccgacac
    14341 cgtcaccctg ctcgacgtcg tcaccgaccc cgacattatc agcccttcga caacgctctc
    14401 cgagagccac tgaaagcttc cctaccgcaa cgttgccatc ttagcagaag gcgacttccc
    14461 ggcactgcgg tgattggggg gcaagaacag gagtggtgca tcctatgcgt gtccgctctt
    14521 cttctttctt gctcgagcag cctcgaagtc aggtatggta ccgaaagtgg gcacgcccga
    14581 cacttgctcg actttctccg gcgaacgctg aggtagaggt actccagtag gaaggccagg
    14641 cctatgccta gcatgagccc tatgaggagc gctccgaacc cgctggagcg gaagtcggtt
    14701 tagcgcgccg cggtacaatg acgccagcga tgggccaaga ggaggtgaag gaccgtagat
    14761 ttcttgagga agcgactgcc tgcccaggtg ggtaagtacc tggagggcgt ggagttcccc
    14821 gcccgcaagg aggagctgct cggaatgctt gagcgcaacg gtgtgccagg cacgatcgtc
    14881 ggccagttgc gcaagaggct cccggaaggc gagtaccgcg gcccccagga cgtagtgtcg
    14941 gccctgcgac gggggcgtta gccgaccgct ggccgtctgc tgcgtcaggc tcgagctttc
    15001 cacggtcgaa tcttcca
//
LOCUS       DASTKM010000169         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_572603, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000169 DASTKM010000000
VERSION     DASTKM010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572603"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1138)
                     /locus_tag="VFQ10_07065"
     CDS             complement(53..1138)
                     /locus_tag="VFQ10_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9965137.1"
                     /translation="MTVEKLRFGILGCGVIGPHHAKAIAGLQSVELVAVADVVPELAE
                     ELADEHGCSPYASLEEMLSGVDLDAVCVCTPSGMHAEGAITALEAGKDVVIEKPVDVT
                     LKAADRLIEVQRATGRRVAVVSQHRFDAGTQAVREALVRGEFGRLTSGSADVRWWRSQ
                     SYYDSGGWRGTWELDGGGVLVNQAIHSIDLLQWLMGEVVEVTAHTGLLAHERIEVEDT
                     AVAILKFEGGALGTILATTAAFPGLTTRIAVHGDRGSAIIDDDELSYFHAAGEGQEGE
                     AYGAGGGENQAEEVMEQYGEAATGPGAGADPGSLSMAHRDQIQDFVEALREGREPLIN
                     LEEGRKPLAIIQGIYESARTGGPVRIE"
     gene            complement(1135..1389)
                     /locus_tag="VFQ10_07070"
     CDS             complement(1135..1389)
                     /locus_tag="VFQ10_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9965138.1"
                     /translation="MNVPDEIAVVGCDDIMLVGLLSTALTTLRVPEYDVGARSARMLL
                     DRTSGRSGESEVLLRPELIVRQSAPRTRDPSRTNTGGEDI"
     gene            1528..1692
                     /locus_tag="VFQ10_07075"
     CDS             1528..1692
                     /locus_tag="VFQ10_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965139.1"
                     /translation="MEGHGGPRIAFVGGGSYQWGPKIIQDVALNEELRGGTLVLHDIN
                     DEALEDMHQW"
     gene            1803..2849
                     /locus_tag="VFQ10_07080"
     CDS             1803..2849
                     /locus_tag="VFQ10_07080"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965140.1"
                     /translation="MDATALDLEIPARYGVVQTVGDTVGPGGLFRRPRNIPVVVEIAR
                     AMEEYCADAVLLNLTNPLTVLTRAVSKATSIVSVGLCHELFSTLGMLSTMFEVPEESI
                     NVRVAGVNHFIWVTEVAVRGRDVTGEAFRRISGGEAREISLSDAEGDTDPFTNTWGFR
                     TELCRHYGYLPAAGDRHVCEFLPGYLRDEKERKRLDLRVTTVDVRRERLAADRERVRR
                     MIRGGEPIPTGRSREEISAIMAAMWTGEDSVNIVNLPNAGQVRDLPPGAVVETYGALN
                     GTGASGIVFGELPPPVAALVHPHVFNQEAIVQAGLTGDMDLAFHAFLNDPLVGSGPDA
                     RKMFEAQREYLPQF"
     gene            complement(2910..>4055)
                     /locus_tag="VFQ10_07085"
     CDS             complement(2910..>4055)
                     /locus_tag="VFQ10_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HET9965141.1"
                     /translation="AWRQRYLDHPVVGTLARRLIWKFTNEERTGAGVFYNGRIVGRDG
                     HVLDWLDDSTSVALWHPMLEERGEVLAWRDWLAELEIRQPFKQAHREVYLLTDAERTT
                     RTYSNRFAAHVIKQHQFNALCAARGWKNKLRLMVDDEYPPAARELPAWGLRAEFWIEG
                     LGENYGTDTTEAGTYLYLATDQVRFYNIDARENYAHAGGGGYAAGFRGQGAPTGPLPL
                     EEVPPLILSEVMRDVDLFVGVASVGNDPNWADGGPEGRYRDYWTSYSFGALSQTAKTR
                     KQVLQRLIPRLKIADRCSLKDRFLIVQGDVRTYKIHLGSGNILMEPNDQYLCIVPTRA
                     PTTGNLGTKLFLPFEGDRTLAIILSKALLLAEDTKISDPTILHQISS"
BASE COUNT          695 a         1260 c         1277 g          823 t
ORIGIN      
        1 cataacccct ttcctggggc ggtgctctcg cggcccgccc cagcttgttc tattactcga
       61 tgcgtacggg cccgcctgta cgggccgact cgtagatccc ttgtattatc gcgagcggct
      121 tgcgcccttc ttcgaggttt atgagcggct cgcggccctc tctgagggcc tcgacgaagt
      181 cctggatctg gtcgcggtgc gccatggaga ggctgccagg gtctgccccg gcgcctggtc
      241 cggtcgccgc ctcgccatac tgctccatga cctcttcggc ctggttctcg cctccgcccg
      301 cgccgtaggc ctctccctcc tgtccctcgc cggcggcgtg aaagtagctc aattcatcgt
      361 cgtcgatgat ggccgatccc ctatccccgt gcacggcgat cctggtcgtt agccccggaa
      421 atgccgccgt cgtcgccagg atggtgccca gggcgcctcc ttcgaacttc aggatggcta
      481 ccgccgtatc ttcgacttcg atcctctcgt gggcgagcag gccagtgtgg gcggtaacct
      541 cgactacctc gcccatcaac cactgaagga ggtcgatgct atggatggcc tggttgacca
      601 agactccccc accatccaac tcccaggttc ctcgccagcc gcccgagtcg tagtaagact
      661 gcgacctcca ccaccgcacg tcggcagacc ccgaggtaag gcggccgaac tctcctctta
      721 cgagcgcctc ccgaacggcc tgcgtgccag catcaaaacg gtgctggctt acgacggcga
      781 ccctccttcc cgtggcacgc tgtacttcga tcaggcggtc cgccgccttt agggtgacgt
      841 cgacgggttt ctcgatgacg acgtccttgc cggcctccag cgcagttatg gcgccctctg
      901 cgtgcatgcc gctaggagtg cagacgcaca cggcgtcgag atccacgccc gataacatct
      961 cttcgaggct ggcgtagggg gagcacccgt gttcatcggc cagctcttcg gccagttcgg
     1021 ggacgacatc ggccacggca accagctcca cactctggag gccggcgatg gccttggcgt
     1081 ggtgcggacc gatgacgccg catcccaata taccgaagcg cagcttctcg acggtcatat
     1141 atcctctccc cctgtgttag ttcgggacgg atcacgtgtt cgcggcgcac tctggcggac
     1201 gataagctcg ggcctgagca agacttcgct ctccccgctg cggccgctgg tgcggtcgag
     1261 tagcatcctg gccgatctcg cgccgacgtc gtactcgggc accctgaggg tggtgagcgc
     1321 cgtcgagagc aggccaacga gcattatgtc atcacacccc accactgcga tctcgtcagg
     1381 aacgttcaag cccaaccccg ctcaggccct caaggctacg acggcgatgg tgagcctgtg
     1441 caccctctcc cgcgaactat cctgatctaa tcggtcacgg tatcataacc tcgggcactt
     1501 atcagcggtg tgtagaggga ggaacacgtg gaagggcatg gcggacccag gatagcgttt
     1561 gtgggtggag gaagctatca gtggggaccc aagatcatcc aggacgtcgc tttgaacgag
     1621 gaattgcgcg ggggcaccct ggtcctgcac gacatcaacg atgaggcgct agaggacatg
     1681 caccaatggt gaacgagagc cctggatgta gcgcgagccg atctggagct ggagaagacg
     1741 cagcggctgg aggaggccct caggggggct gacttcgtgg tgttgagcat ctcgaccggg
     1801 gcctggacgc tactgcactc gacctggaga tccccgcccg ttacggtgtg gtgcagaccg
     1861 tcggcgacac cgttgggcct ggcggtctgt tccgcagacc gcggaacatc ccggtggttg
     1921 tggagatcgc acgcgccatg gaggagtatt gcgcagatgc cgtccttttg aacctcacca
     1981 acccactaac ggttctcacc agggccgtca gtaaggctac ttccatagtg tctgtaggtc
     2041 tctgtcacga gctcttcagc accctcggca tgctctcgac gatgttcgaa gtgcccgagg
     2101 agtccatcaa cgtgagggtg gccggggtca accactttat ctgggtcacg gaagtagctg
     2161 tgcgtgggcg cgacgtaacg ggcgaggcgt tccgcaggat ctcaggcggc gaggcgcgtg
     2221 agatctcact ttcagacgcc gagggggaca ccgacccctt tacgaacacc tggggcttcc
     2281 gcaccgagct gtgccggcat tacgggtatc tgccggcggc tggcgaccgc cacgtgtgcg
     2341 agttccttcc cggatacctg cgcgacgaga aggagcggaa gcggctcgac ctcagggtga
     2401 ccacagtaga tgtcaggagg gagcggctcg ccgcggacag ggagagggtg aggcgtatga
     2461 tccggggcgg agagccaatc ccgacagggc gctcgcggga ggagatctcc gccatcatgg
     2521 ccgccatgtg gaccggcgag gactctgtga acatcgttaa cctgccgaac gccggccagg
     2581 tcagggactt gccgccagga gccgtagtcg agacctacgg cgcgctcaac ggcaccgggg
     2641 cttctggcat tgttttcggg gagcttcccc caccggtggc cgcgctggtc cacccccacg
     2701 tcttcaacca ggaggccata gtgcaggccg gtttgacggg agacatggac ctagcgttcc
     2761 acgccttcct gaacgatccc ctcgttggta gcgggcccga tgccagaaag atgttcgagg
     2821 ctcagagaga gtatctgcca cagttttagc agttctagca tttcagcaca ctccagcctt
     2881 cggctgtcgc ttgtcagcat ttctgcttgt cagctggaga tctggtggag gattgtgggg
     2941 tcgcttatct tggtgtcttc tgctaacagt agtgctttgc tgaggattat tgccagggta
     3001 cggtcgcctt cgaagggtag gaatagcttg gttcccaggt tacctgtggt tggtgcgcgc
     3061 gttgggacga tacacaggta ctggtcgttt ggctccatca gtatgttgcc gctgccgagg
     3121 tgaatcttgt atgtgcgtac atcgccctgg acgatcagga acctgtcttt caggctgcag
     3181 cggtccgcta ttttgaggcg ggggatcaga cgttgcagca cctgcttgcg cgtctttgcg
     3241 gtctgggaga gggcgccgaa ggagtagctc gtccagtaat ctctgtaacg gccttctggt
     3301 ccgccgtcag cccagttcgg gtcgttgccg acgctggcta ctcccacgaa caaatccacg
     3361 tcgcgcatca cctcggagag tatgagtgga ggtacctctt cgagagggag tggtcctgtg
     3421 ggagcgcctt ggccacgaaa tccggcggcg tacccacccc cgccagcgtg agcgtagttc
     3481 tccctggcgt cgatgttata gaagcggacc tggtctgtgg ccaggtagag gtaggtcccc
     3541 gcctctgtgg tgtcggttcc gtagttctca ccaaggcctt cgatccagaa ttcggccctc
     3601 aatccccagg ccggcagttc ccgggccgca ggcggatatt catcgtcgac catgagcctg
     3661 agcttgttct tccacccccg cgctgcgcac agggcgttga actggtgctg cttgatgacg
     3721 tgggcggcga agcgattgga gtaggtcctc gtggtccgct ccgcgtcggt gagtaggtag
     3781 acttccctgt gggcctgctt gaagggctgg cgtatctcca actcggcgag ccagtccctc
     3841 caagcgagga cctcgccccg ttcctccagc ataggatgcc acaacgctac gctggtcgag
     3901 tcgtcgagcc agtccaggac gtggccgtca cggcccacga tccggccatt gtagaagacg
     3961 ccggctcctg tgcgttcttc gttcgtgaac ttccagatca agcgccgcgc gagcgtgccg
     4021 acgacgggat ggtcgagata gcgctggcgc caggc
//
LOCUS       DASTKM010000170         4540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_396708, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000170 DASTKM010000000
VERSION     DASTKM010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396708"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFQ10_07090"
     CDS             <1..381
                     /locus_tag="VFQ10_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HET9965142.1"
                     /translation="MVLELARGQNFAALTTLLPGGHPQTQVMWIDANEQHLLVNTEVH
                     RQKFRNVERDPRVTVTIWDMEDPYRFVEVRGEVVEKVKGQEAREHIDELSHKYRGKPY
                     QPQIKSERVVLRIASSRQVIRIPS"
     gene            664..1035
                     /locus_tag="VFQ10_07095"
     CDS             664..1035
                     /locus_tag="VFQ10_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965143.1"
                     /translation="MTTRKPELPTIPFASRQAWETWLEEHHASSDGLWLKFAKKDSGI
                     ETVSFAEALDLALCYGWIDSQADSFDDEYWLVRFTPRRPKSKWSKVNRGKVSKLIEEG
                     RMKPAGLREVERAKADGRWEE"
     gene            1060..1842
                     /locus_tag="VFQ10_07100"
     CDS             1060..1842
                     /locus_tag="VFQ10_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET9965144.1"
                     /translation="MTGERHLQRHVGKIARWIAASEHTVAFTGAGISTDSGIPDFRGP
                     EGVWTRRDAGLPAPRWRVPPGEVAPNASHLSLFELQRLGKLRFLVTQNTDNLHRRSGI
                     SPEFLAELHGNGQLMRCQGCDRLFTRQEVGWDTGRWGPGYRTQKPVPGQPQCANCGGR
                     LISSVVNFGDPLPERVLMLADHHARRCDLMLVLGSSLMVQPAASLVGLALRSGARVVL
                     VNRGKTPYDRAVTLRVWTGIGEVIPPAVEKVKGALGEQSNMP"
     gene            complement(1869..3374)
                     /locus_tag="VFQ10_07105"
     CDS             complement(1869..3374)
                     /locus_tag="VFQ10_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9965145.1"
                     /translation="MRNRTLTRRDFLKMTGAGVASATLLGATGCDLTERIRNITVDPP
                     GVTEGTNVVLIIVDSLRKDHIGAYGNDKIRTPNLDALAKDSLLFTRAYPESIPTICAR
                     RAIHTGFRTWPFRDWVPPKGEDIILQGWQPIPNYQTTLAEMMQAAGYGTYLVTDNMHQ
                     FKPSYNIHRGFDVFDFFRGQTTDNYRPAWTYPKDKVARALLKGNIPAMTAQMRQYFAN
                     VKERKTEADWFSPMVFSQAADYLEPLSKGGPFFLTVDSYDPHEPWDPPESYVKMYDDE
                     PYNLKEPFSVIYGPSSYLLPRELERMKARYSAEVTMMDRWLGRFLDKMDELGLFENTL
                     LILLSDHGVAYGEHGYTGKPDNVLWPEVTDIPFYMRHPEGKGAGQKSDYYASTHDVAP
                     TILGALGIEPRQELDGQDLSVMLGDGEPEARPHFSIGYNDHVWARDDRYVMFSTNQGA
                     DAKLYDLQQDPGMHNNLAGVEPNRVKRMFEGYILRDAGGPLPTYDYGTAAR"
     gene            3481..3915
                     /locus_tag="VFQ10_07110"
     CDS             3481..3915
                     /locus_tag="VFQ10_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965146.1"
                     /translation="MRSIVAVGIGLLLALVLGLLVVQGIFAPVLTRFFGLERAGPAAL
                     PLVLLVFAAAFSFYFGGMAASYKAPSRHRLHGVLVVPVAFVLSLVLNLVLGKGFLPGV
                     DGSWAVGLVAVFLVVSGAASYVGALRGEALYAHNQKLARPRR"
     gene            complement(3920..>4540)
                     /locus_tag="VFQ10_07115"
     CDS             complement(3920..>4540)
                     /locus_tag="VFQ10_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965147.1"
                     /translation="ITELEGALEESEARRNELETVLEEIRAGGEESSGITVWPDDAAD
                     GEPKEPLEDVEAQNILAEKKVEALEVKLSEARAESRRNAEELQRALESLDRLSDPSRR
                     LREGIALFNESEHARTVASISKAFGLPRVHAALDDTSPGRPTLTFLWGDMAWRRYVSD
                     PTVGVEEPRVYLTGTGEDPAEVEASSRQPNARMDSRGRLMIGVQAR"
BASE COUNT          779 a         1373 c         1449 g          939 t
ORIGIN      
        1 atggttctcg aactggcccg tgggcagaac tttgcggccc tcactactct gttacccggc
       61 ggccacccgc agactcaggt gatgtggatc gatgccaatg agcagcacct cttggtcaac
      121 accgaggtgc accgccagaa gtttcgcaac gtcgaacgcg acccgagggt gacggtcacg
      181 atctgggata tggaagaccc ctatcgcttc gtcgaagtgc gcggagaagt cgtagagaag
      241 gtgaaaggtc aggaggcgag agagcacatc gacgaattgt cccacaagta ccgtggcaag
      301 ccttaccaac cacaaatcaa gagtgaaagg gtggtgctcc ggatcgcttc ctcccgacag
      361 gtgattcgca taccttcctg actcacccga cagatgggcc ttcagctctg ctgcggctgg
      421 catgtgaggg cgactccatc acgccatcca gcgacaccaa ctccacagcc acgactttcc
      481 tcatggatat ccttccgccg ttttagatcg tctggcctta gcataaggtg atatacctga
      541 aactcaaccg gcatccttcg gagtcctctt gtaccgggag gtcgatgaat agtcgggaga
      601 ggatgggaac ttccacggtc cttctcggcg gacagcggca ccagtgatcg aaagggagga
      661 gccatgacca ccagaaagcc cgaactgcct acaattccgt tcgcctcccg ccaggcgtgg
      721 gagacgtggc tcgaagagca ccatgcgagc tcggatggac tgtggctcaa gttcgccaag
      781 aaggactcag gtatcgagac ggtctccttc gcagaggccc tggacctcgc cctctgctac
      841 ggttggatcg acagccaggc ggacagcttc gacgacgagt attggctggt tcggttcacc
      901 ccgcgccggc cgaagagcaa gtggtcgaag gtcaaccgcg gcaaggtgag caaactgatc
      961 gaggaaggca ggatgaaacc cgccggtctt agggaggtgg agcgtgccaa agccgatggc
     1021 cgctgggagg agtaatctag ctgctatgcg gcggatacca tgaccggcga acgacacctc
     1081 cagcggcacg tcgggaagat cgcccggtgg atcgccgcct cagagcacac cgtcgctttc
     1141 acgggcgccg gtatcagcac cgactcgggc atccccgact ttcgtggccc cgagggggtg
     1201 tggactcggc gcgacgccgg cctgccggca ccgaggtggc gggttccgcc gggtgaggta
     1261 gcgcccaacg cctcccacct gtcactcttc gaactccagc ggctcggcaa gctccggttc
     1321 ctcgtcacgc agaacacaga caacctgcat cgccgctccg gcatcagccc cgagttcttg
     1381 gcggagttac acggcaacgg gcagctcatg cgctgtcagg gctgcgaccg gctcttcacc
     1441 agacaggagg tagggtggga cacagggcgg tggggtcctg gttaccgcac ccagaagccg
     1501 gtgcccggcc agccgcagtg cgccaactgc ggcgggcgcc tcatctcctc ggtggtgaac
     1561 ttcggggacc cgctgcctga gagagtgttg atgctggccg accaccacgc ccgccgctgc
     1621 gacctgatgc tcgtcctcgg ctcctcgctc atggtgcagc ctgcggcctc gctcgtaggg
     1681 ctggcgctca ggtcaggagc ccgggtggtc ctggtcaacc ggggcaaaac accctacgac
     1741 agggctgtga cgctgcgggt atggacgggt attggtgagg tgatcccgcc cgcagtggag
     1801 aaggtgaagg gggcactcgg ggagcagtcg aacatgcctt gaatacaggg gtatgacgct
     1861 ggagcgctct atcttgcggc ggtgccgtag tcgtaggtgg gcagggggcc gccggcgtcc
     1921 ctgagtatgt agccttcgaa catacgcttg actctgttgg gctcgacccc cgccaggtta
     1981 ttgtgcatgc ccgggtcttg ctgcaagtcg tagagtttgg cgtccgcgcc ctggttggtg
     2041 ctgaacatga cgtagcgatc gtcgcgggcc cagacatggt cgttgtagcc gatggagaag
     2101 tggggtctcg cctcgggttc tccgtctccg agcatgacgg agaggtcctg gccgtccagt
     2161 tcctgcctgg gttcgattcc cagggcgccg agtatcgttg gggccacgtc gtgggtagag
     2221 gcgtagtagt cgctcttctg tccagcccct ttgccttcgg ggtgcctcat gtagaacgga
     2281 atgtcggtaa cctcaggcca gagcacgttg tctggcttgc ccgtatagcc gtgctcgccg
     2341 taggcgacgc cgtggtcgga gaggagtatg aggagggtgt tctcgaagag gcctagctcg
     2401 tccatcttgt cgaggaaacg gcccagccag cgatccatca tcgtgacctc cgccgagtag
     2461 cgcgccttca tcctttcgag ctctcttggt agcaggtagc tgctcggacc gtagatcacc
     2521 gagaacggct ccttgaggtt gtaaggctca tcgtcgtaca tcttgacgta gctctcgggc
     2581 gggtcccagg gctcgtgcgg atcgtagctg tccacggtaa ggaagaaagg cccgcccttg
     2641 ctcaagggtt cgaggtagtc tgcggcctgg gagaagacca tgggggagaa ccagtcggcc
     2701 tccgtctttc gctccttcac gttggcgaag tactggcgca tctgagctgt catagcgggt
     2761 atgttgccct tgaggagggc ccgggcgacc ttgtccttgg ggtaggtcca cgccggcctg
     2821 taattgtctg tggtctggcc gcggaagaag tcgaagacgt cgaagccacg atgaatattg
     2881 taggatggct tgaactggtg catgttgtcc gttactaagt aggtcccgta ccccgccgcc
     2941 tgcatcatct cagcgagggt tgtctgatag ttgggtatcg gttgccagcc ttgcaggatt
     3001 atgtcctcac ccttgggcgg tacccagtct ctgaagggcc aggtcctgaa gcccgtatga
     3061 atggccctcc tcgcgcagat cgtcggtatg gactcaggat atgcccgcgt gaagagcagg
     3121 ctgtctttgg ccagggcgtc aaggttgggg gtcctgatct tatcgttgcc gtaggcgccg
     3181 atgtggtcct tcctcaagct gtcgacgatg atcagaacca cgttagtgcc ctctgttacg
     3241 ccagggggat cgacggtgat gttacggatg cgctctgtaa ggtcgcagcc tgtggcaccg
     3301 agcagagtcg ccgaggccac cccggcgccg gtcatcttga ggaagtctct gcgggtcagc
     3361 gtgcggtttc tcacggagca agagcgtact acgcaacaat gccacctaca agccgaacgg
     3421 gcgggccgtt accgtgaacc cggctcttca gagactgggg ggactgctag aataaccttc
     3481 atgagaagca tcgtggccgt tgggatcgga ttgctactgg cgctcgtgct cggtctactc
     3541 gtggtacagg ggatattcgc gccggttctc acacggttct tcggcctcga gcgcgcaggc
     3601 cccgccgccc tcccgctcgt cttgctcgtc tttgccgccg ccttctcgtt ctacttcggg
     3661 gggatggccg ccagctacaa ggcgccatcc aggcatcggc tgcacggggt tttggtcgtc
     3721 cccgtagcct tcgttctttc gttggtgctc aacctggttc ttggcaaagg atttttgccc
     3781 ggcgtcgatg gttcctgggc cgtgggactg gtggccgtct tcctcgtcgt ctctggcgcg
     3841 gcctcctatg ttggggccct ccgcggggag gcgctctacg cccacaacca gaagctagcg
     3901 cggccccgcc gttagcgctt cagcgggcct gcacgccgat catcaaacgc cctctggagt
     3961 ccatcctggc gttgggctgc cggctggacg cttcgacttc ggccgggtct tcacccgtac
     4021 ccgtgaggta gaccctgggt tcttcgaccc ctactgtcgg gtcggagacg tagcgccgcc
     4081 aggccatgtc gccccagagg aacgtgagag ttggtctccc tggagacgtg tcgtccagtg
     4141 ccgcgtgaac cctagggagg ccgaaggcct tcgagatgga cgctactgtc ctcgcgtgct
     4201 cgctctcgtt gaacaacgcg atgccctccc tcagacggcg ggatggatcg gagagccggt
     4261 cgaggctttc cagggctctt tgtaactctt cggcgtttcg ccggctctcc gctctggcct
     4321 cagagagctt cacctccagg gcttcgacct tcttctcggc cagtatgttc tgcgcctcga
     4381 catcctccag aggctctttc ggttctccgt ctgcggcatc gtcgggccag actgtgatcc
     4441 cggacgactc ctcaccgccg gcacggatct cctcgagtac ggtctccagt tcgttcctgc
     4501 gggcctcgga ctcttcgagg gccccttcca gctcggtgat
//
LOCUS       DASTKM010000171         5905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_44805, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000171 DASTKM010000000
VERSION     DASTKM010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44805"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFQ10_07120"
     CDS             <1..241
                     /locus_tag="VFQ10_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965148.1"
                     /translation="PMKISRRTANILLAIGVYMLLTWGTRVFTFLAEFRAGTLVAPAL
                     HFSLVVIGLAIGVYLAYLGIKGRKHAPAVRPERAD"
     gene            complement(245..2200)
                     /locus_tag="VFQ10_07125"
     CDS             complement(245..2200)
                     /locus_tag="VFQ10_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HET9965149.1"
                     /translation="MWLLQRIGERLLDRVDRLQRAFHRSPPAFRRGAAMLVDALIVIE
                     SFAVTLIVRVAGDTAGPPFWPFFWPFAVFSALAFVLLLNANGVYQSILRYTGIYQGVR
                     VLSATAMAAGGLSLTVICVGPWGFGLMDFNPVPLSVPLMGSVLAYVQLVAVRLYPRVF
                     YEMSLREVGRRTRTVIVGTGEPGVALAGHIWRTAASETQVVGFVSRSPVEVGRHIEGS
                     PVLGVVEDLEELIAQHGIDQVIIAMPQASREEMDWIWRTSIRASAEVKVMPDLGEYLA
                     EGAIKLRELQIEDLLGRESVDIDLDALSDYINSKRVLVTGAGGSIGKELSRQISRLGP
                     AKLILLDRDESGLYYLNTELRREDFYDTEVFVGDVTHSERVSFAFETFRPQLVFHAAA
                     YKHVPMMEQQATEAIVNNVLGTLNVARAAGAYGTSKFINVSTDKAVSPVNVMGATKRL
                     AEMVVRELSLQYPETIYASVRFGNVLGSRGSVVPTFRQQIEAGGPVTVTHPDMIRYFM
                     TIPEAVSLILQAGAMAEGYGTYVLEMGRPVAITDLARKMIEIMGAPNVSIKFVGLRPG
                     EKLKEELSEEGEQRDTTAHPMVFRLSSENVSPPGDSNLSELIDAMVFHARGQEGGRAL
                     QLLRRAVPNYSAVDLPEVTESRLDYPL"
     gene            complement(2429..3409)
                     /locus_tag="VFQ10_07130"
     CDS             complement(2429..3409)
                     /locus_tag="VFQ10_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965150.1"
                     /translation="MIGVVAGGVAFLVAGAFVPLLVRFAVGRNLLDVPNLRSSHDVPT
                     PRLGGVAIFLGMLSGVIILGPVGLWPLLAAAALVWAVGLADDLSNLHFGVKAVLQALA
                     AVGLLLLYPPTILSDAPGLLGVLVLAVGVFWLVALSNAFNFMDGIDGITGGVALVNAL
                     FLAPLVGQAGGFLPALIGATAAFLIWNISPASIFLGDSGAYLIGFSLAAVALYAPALS
                     GQEWTPLGFLACVVVFTPYLFDTAYTLVRRLRSGAGKSIFLAHREHIYQRITPTTGMH
                     RRTSNLYYGASVIAGFAALLISRGDVLSFVGVALALACCVLLAALPKIVS"
     gene            complement(3603..4481)
                     /locus_tag="VFQ10_07135"
     CDS             complement(3603..4481)
                     /locus_tag="VFQ10_07135"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9965151.1"
                     /translation="MRILLTGATGLLGSALLRLLLARGHETRCFVRADSPNTSRLDGE
                     RVEILHGDAAREEDLYRALRDVDAILHVAGIEYAPSVVRAAGRAGVERVLIVGSTSAH
                     SAYEFRSGPKLRMEELVRGSSLAWTILRPTMIYGSERDRNVHRLLRFLDRSPVFPIFG
                     PGTNLWQPVYHEDCARGVFEALERPTSVRRSYDLPGADSLAYLDLVKTAAAALGRSPR
                     IVRLPIEPVRLALAAAERLRLPLPVDSGQVMRLREDKAYPYEKAWRDLDYTPRPFREG
                     VGLEVARLRRLGMVRS"
     gene            complement(4530..5012)
                     /locus_tag="VFQ10_07140"
     CDS             complement(4530..5012)
                     /locus_tag="VFQ10_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965152.1"
                     /translation="MHHRGLTTVLFLATIVLIAGLLTGCGDQSGSGSQQDGASKAEQG
                     KNAPVGQGENVPKKQGKNAPKKKILFGTVTFVNTKTEGFSVRPTIDEQGNKPVAFRLA
                     NESSITLGGKEAKLEDMKKGQQAQVEYVERNERNRARSVRLFEAGGGSQDDQGGEKTG
                     "
     gene            complement(5075..>5905)
                     /locus_tag="VFQ10_07145"
     CDS             complement(5075..>5905)
                     /locus_tag="VFQ10_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9965153.1"
                     /translation="FIWVFGVARTGSSWLGAMMGDLDDHAIWYEPYVGDVFGYAYYMR
                     AGEQQRRREDYILGDPYRMAWIRSLRTFVLEGAHARFPELGENGFLVVKEPNGSVGAP
                     LLVEALPESRVILLVRDPRDVVASLLAAQKEGSWGAGEDALADTDPDEFVRQRARMYN
                     ASFGKAWEAYEAHRGRKVATRYEDLRYDTLGELEKVYSSLAIPIEKERLRRVVEKHAW
                     ENIPERQKGPNKPRRKAKPGGWREDLTPEQARMVEEITTPTMNEFYPGWERAGATPSD
                     "
BASE COUNT         1090 a         1923 c         1803 g         1089 t
ORIGIN      
        1 gccaatgaag atcagcaggc gcaccgcgaa catcttactt gcgataggcg tctacatgct
       61 cctcacctgg ggtacgaggg tctttacctt ccttgctgag ttccgtgcgg gaacgctagt
      121 cgcgcctgcc ttacacttct cgctcgtcgt gatcgggctc gccatcggcg tctaccttgc
      181 ctacctcggc atcaagggca gaaagcacgc tccggccgta cggccggagc gtgctgacta
      241 gctcttacag cgggtaatcg agcctgctct ccgtgacttc gggcagatcc acggccgagt
      301 agttggggac ggcacggcgt agaagttgga gggcccttcc tccctcctgg ccgcgggcgt
      361 gaaacaccat agcatcgatg agttcagaga ggttcgagtc accgggcggg ctcacgttct
      421 ccgaggaaag cctgaagacc atcggatggg ctgtcgtgtc cctttgttcc ccctcctccg
      481 agagctcctc tttcagcttc tcgccggggc gcaggccgac gaactttatc gagacgttcg
      541 gcgcgcccat tatctcgatc atcttccgtg ccaggtcggt gatggcgacg gggcggccca
      601 tctcgaggac gtaggtgccg tagccctcgg ccatcgcccc ggcctgcagg atcagggaga
      661 ctgcctcggg gatggtcatg aagtacctga tcatgtcagg gtgcgtgacc gttacggggc
      721 cgccggcctc tatctgctgg cggaaggtcg gaacgacgga gccgcggcta ccgagcacgt
      781 tcccgaagcg gacagaggcg tagatcgtct cggggtactg aagggagagt tccctaacga
      841 ccatctcggc caggcgtttg gtcgcgccca tcacgttgac gggacttacg gccttgtcgg
      901 tcgagacgtt gatgaacttg ctcgtcccgt aggcccccgc cgcacgggcg acattgaggg
      961 tgccgaggac gttgttgacg atggcctcgg tcgcctgctg ctccatcatg gggacgtgtt
     1021 tgtaggcggc cgcgtggaag acgagttgtg gcctgaaggt ctcgaaggcg aagctgacgc
     1081 gctctgaatg cgtaacatcg ccgacgaaga cctccgtgtc gtagaagtcc tcgcgcctca
     1141 gctccgtgtt caaatagtac aggccgctct cgtcccggtc gagcaggata agcttcgcag
     1201 ggccgaggcg tgagatctgg cgtgagagct ccttgccgat ggagccccct gctccggtca
     1261 cgaggacccg cttactgttg atgtagtccg agagggcgtc gaggtctatg tccacggact
     1321 cgcgtccaag gaggtcttcg atctgaagct cgcgcagctt gatggcgccc tcggccagat
     1381 actccccgag gtctggcatg accttcacct ccgcggaggc gcggatggag gtgcgccaga
     1441 tccaatccat ctcctctctg ctggcctggg gcatggctat gatgacctgg tctatgccgt
     1501 gctgggcgat aagctcttcg aggtcctcta ccaccccgag gaccggcgag ccctctatgt
     1561 gcctgcctac ctcgacggga gacctgctga cgaagccgac gacctgtgtc tcggacgcag
     1621 ccgtgcgcca gatgtgcccg gcgagcgcca cgccaggctc gccggttccg acgataaccg
     1681 tccgcgtccg gcgcccgacc tccctcaagg acatctcgta gaagacccga ggatagagcc
     1741 gcacggcgac gagctgcaca taggcgagta cggaccccat caacggtaca gagagaggga
     1801 cggggttgaa gtccatgagg ccaaaacccc agggacccac acagattacc gtcagggaga
     1861 ggccgccggc cgccatcgca gtcgcgctca gaacccgcac cccctggtag atgccggtgt
     1921 agcgcaggat gctctggtag accccgttgg cgttcaggag cagcacgaac gcgagcgccg
     1981 agaacaccgc gaaaggccag aagaacggcc agaacggggg acccgccgtg tctcctgcga
     2041 cgcggacgat gagcgtaacg gcgaaagact cgataactat gagggcgtcg acgagcatcg
     2101 ccgccccgcg cctgaacgcc ggcggcgagc ggtggaaggc cctctgcaac cggtccacac
     2161 gatccaggag cctctccccg atcctctgca aaagccacat aggtctgtat attaactttt
     2221 gttggcggat ctaaccacct ttcaagctag tcagtatgtc agcacgctcc ggcttcacgg
     2281 ccttcttcgc tcgtctgcat ttcagcaaga gcgcgcagag cgcgactagc tccgaaagag
     2341 gcatttctgc tcatggaatc ctactttgcg gccgtgagcg aggccattag cggaaagagc
     2401 tgacaaggcg ccaaaggcgc cgtgctgact agctcacaat cttcggcagc gctgccaaca
     2461 ggacgcagca cgccagcgcg agcgcgacgc ccacgaacga caggacgtct cccctggaga
     2521 tcagcagtgc ggcaaaaccg gcgatgacgc tcgccccgta gtagagattg ctcgtacgcc
     2581 tgtgcatccc cgtagtcggg gtgatccgtt ggtagatatg ctccctgtgc gccaggaaga
     2641 tgctctttcc agccccgctc cgcaaccgcc gaaccagcgt atatgcggtg tcgaagaggt
     2701 aaggcgtgaa gacgaccacg caagccagaa agcccaaagg cgtccactcc tgccccgaga
     2761 gcgcgggagc gtagagggcc acagcggcaa ggctgaagcc gattaagtat gctccagaat
     2821 ccccaagaaa gatggatgca gggcttatgt tccagatcag gaacgccgca gtagccccga
     2881 tgagcgcggg caggaaaccg cccgcctgtc ccacgagcgg cgcgaggaag agcgcgttga
     2941 cgagcgcgac gccccccgta atgccgtcta tgccgtccat gaagttgaag gcgttgctta
     3001 gggccacgag ccagaacacc ccgacagcga gaaccagaac gccgagcaac ccaggagcgt
     3061 ccgagaggat cgtcggcgga tacaacagca gcaatcccac tgcggccaga gcctggagga
     3121 cggccttcac gccgaagtgg aggttggaca ggtcgtcggc gagtcctacg gcccatacaa
     3181 gtgccgccgc agcgagcagc ggccacagac ccaccgggcc aagtattatc actcctgaga
     3241 gcatcccaag gaatatggct acgcccccaa gcctgggtgt cgggacgtcg tgcgagctgc
     3301 ggaggttggg cacgtcgagc agattccgtc cgacggcgaa cctcacgagc agtggtacga
     3361 acgctccggc caccaggaaa gccacgccac cggcgacaac cccgatcacc atggctccca
     3421 cgccttacac aagcgggttc tcaataccct cgacagagag gccgtggggc atgccttccg
     3481 ttagcgaccc gccgtcgcca gcccagagag tccttgcccg ccttactttc tctaccgtga
     3541 tcgccagcct ggccacgcct actcctctcg cgccacacga tttcagcata gcaccctaag
     3601 tactacgatc gcaccatccc gagtctccgc aggcgcgcga cttcgaggcc tacaccctcc
     3661 ctgaagggac ggggcgtgta gtcgaggtcg cgccaggcct tttcgtaagg gtaggccttg
     3721 tcttcacgca gccgcatcac ctggcctgag tcgacgggca gaggtagccg cagcctctcc
     3781 gcggcggcga gcgccaggcg gaccggttcg ataggcaatc tgacgatgcg tgggctccta
     3841 cccagggccg ctgccgcggt cttcaccagg tcgaggtaag caagggagtc ggcgcctggc
     3901 agatcgtagc tgcggcgtac ggacgtaggg cgttcgaggg cttcgaatac gccgcgtgcg
     3961 cagtcttcgt ggtagacagg ctgccagagg ttcgtgccag ggccgaagat ggggaagacg
     4021 ggcgagcggt cgaggaagcg aagcagacgg tgcacgttcc tgtcccgctc ggagccgtag
     4081 atcatcgtag ggcgaagtat cgtccatgcg agagagctcc cacgcacgag ttcctccatc
     4141 ctgagcttcg gaccggaacg gaactcgtag gccgagtgcg cgctcgtgct gccgacgatg
     4201 agaacccgtt cgacgccagc ccggccggca gcccgtacga cggagggagc gtactcgatc
     4261 ccggcgacgt gcaggatggc gtccacgtcc ctgagcgccc ggtagagatc ctcctctctg
     4321 gctgcgtccc cgtggaggat ctccaccctc tccccgtcga gcctggaagt gttgggactg
     4381 tcagcgcgga cgaagcagcg cgtttcatgg ccccgagcga gcagcagcct gagcaatgca
     4441 ctcccaagca aacccgtggc gcctgtgagg agtattctca tccgaaccag ggacgtggtg
     4501 gctctcgagt gcgtccttct ttgtggccct cagccggtct tctcaccacc ctggtcgtcc
     4561 tgggagcctc cgccggcctc gaacagtctc acgctgcgag cccggttacg ttcgttcctc
     4621 tctacatact cgacctgcgc ctgctggccc ttcttcatgt cctccagctt cgcctccttg
     4681 ccgccgagcg tgatgctgct ctcgttcgcg agcctgaaag ctacaggttt gttgccctgt
     4741 tcgtctatgg tcggccgcac gctgaacccc tcggtcttgg tattgacgaa cgtgaccgtg
     4801 ccaaacagta tcttcttctt cggcgcgttc ttaccctgct tcttcggtac gttctcgccc
     4861 tgtcccaccg gagcgttctt gccctgctcc gctttggatg cgccgtcctg ctgcgaccca
     4921 ctccctgatt gatcgccaca gcccgtgagg agtccggcta tcaaaacgat cgtggccagg
     4981 aagagcaccg tggttagtcc tctgtggtgc atcgagtctc cttgtcctgc cgtgtgcttt
     5041 gcggggccaa actaccagaa agagcgaaag gccgtcaatc ggatggtgtg gccccggcac
     5101 gctcccagcc agggtagaac tcgttcattg tgggggtggt gatctcctcc accatcctcg
     5161 cctgctccgg cgtcaggtcc tcccgccacc cgccgggctt ggccttgcgg cggggcttgt
     5221 tcggcccctt ctgtctctcg gggatattct cccaggcgtg cttctcgacc acccttcgca
     5281 gtcgctcctt ctcgatagga atagcgagcg aggagtagac cttctccagc tcgccgagag
     5341 tgtcgtacct gagatcctcg tacctggtcg cgaccttgcg ccctcggtgt gcctcgtaag
     5401 cctcccacgc cttcccgaag ctcgcgttgt acatcctggc ccgctgccgt acgaactcgt
     5461 cagggtcggt gtcggccaga gcgtcctcgc cggcgcccca gctcccttct ttctgggcgg
     5521 ccagaagcga ggcgacaaca tccctcgggt cgcggacgag caggatcaca cggctctcgg
     5581 gaagggcctc tacaaggagc ggcgcaccca cagagccgtt cggttccttt acgaccagga
     5641 agccgttctc gcccaactcg gggaagcgcg cgtgggcccc ttccaggacg aacgtcctca
     5701 aggatcttat ccaggccatt ctgtaagggt cgccgaggat gtagtcctcc cttcttctct
     5761 gctgctcccc ggcgcgcatg taataagcgt agccgaacac gtctcccacg tagggttcgt
     5821 accatatagc gtggtcgtcc agatctccca tcatggcgcc gagccagcta ctgccggtcc
     5881 tcgcgacgcc gaagacccag atgaa
//
LOCUS       DASTKM010000172         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_573453, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000172 DASTKM010000000
VERSION     DASTKM010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573453"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1256
                     /locus_tag="VFQ10_07150"
     CDS             258..1256
                     /locus_tag="VFQ10_07150"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HET9965154.1"
                     /translation="MASAAVEYAAVTERVALEVARLEGSGDSERAYTLATKAFREELE
                     KVPVSGRVTVLREQRLDEKGRVRAPKSHPGIVSVDDSLGNRGPEADLAVEPIEGLQLL
                     VKGQEGSISAVAAAPRGTMLRTPDMYMSKLIVGPGAKGNIDIDAPVSENIEAIAEALG
                     RRPQEITVAVLDRDRHETLIEEIRKTGARIQIRPEGDLTPAIAVGMRGADPHAIIGIG
                     GAPEGILAAAVMKCLGGEMQARMWPTQRSQVEKLEEYGFTDPERKLTLDDLIRGTDVL
                     FAATGITPGEPLNGVRYFRGGARTHTVVMSTEPRVVRFMDTIHALEPEAGRRGFQL"
     gene            1315..2832
                     /gene="rho"
                     /locus_tag="VFQ10_07155"
     CDS             1315..2832
                     /gene="rho"
                     /locus_tag="VFQ10_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET9965155.1"
                     /translation="MTERSTLERKRLSDLHQIASQLGIKQYRKYRKPELAEMIYKVAT
                     EQAASQQNETTTNGGATATATREAPVTTDGPNGATETPVAPVDGFGEADTNLATTEQV
                     ERQRQERGRRDRKNGRQDRRQEQRGDRDGKGGEENVQSGILDVLPDGFGFLRTGGYSQ
                     SKGDVYVSTSQIKRFNLRRGDYIVGQIRPAREGEKYPAMVRIETVNGQEPQKGRWRPN
                     FDDLTPLYPMERLRLEWKPNDIAPRVIDLVAPIGKGQRGMVVSPPKAGKTTILKQIAQ
                     SIAANYPETKLFVVLADERPEEVTDWQRSVEEAEVVASTFDQPADNHIAVAELVLERV
                     KRLVEEGEDVVVLLDGITRLSRAYNLAAPASGRILSGGVDSAALYPPKKFFGAARNIE
                     NGGSLTILATALVETGSRMDEVIFEEFKGTGNMELRLDRKLANRRIYPAINIEDSSTR
                     KEELLMEPAEAQRVWQVRSILNALDNAQKIELLIQKLKETRTNPEFLRELQRVRG"
BASE COUNT          662 a          905 c          985 g          453 t
ORIGIN      
        1 gtgggcgagg gcctcgacca ggagctgctc gggctcgcgc gggaggcggt ggcgaacagc
       61 gcgccgtgag ccggtggccg acgtactgac gctggtgtat aatcagagtg tcaggtacac
      121 tggttccctg gaaccactcc gcaccgtttc gaagcaggcc caatcacgag agtgctctgt
      181 ggcgtgtggt ctatcttgat gggtaatggg aggttcggtg agagcccatt aacgttactg
      241 attcccactc gggaaccata gcttcggctg ccgtcgaata tgccgccgtg accgagcggg
      301 tcgcgctcga ggtggcgcgc ctcgaaggtt ctggagactc cgagcgtgcc tacacgctcg
      361 ccaccaaggc tttccgcgag gagctggaga aggtcccggt atcaggaaga gtaacggtgc
      421 tgcgcgagca gcgtctcgac gagaaggggc gggtcagggc gccaaagagc catcctggga
      481 tagtcagcgt agacgactcg ctcgggaaca gagggccgga agccgacctg gcagtcgagc
      541 ccatcgaggg cttgcagttg ctcgtaaagg ggcaggaggg ttcgatctcg gcggtcgcgg
      601 ctgccccgag ggggaccatg ttgaggaccc cggatatgta catgagcaag ctgatcgttg
      661 ggcctggagc aaagggtaac atcgacatag acgctcccgt ctccgagaac atagaggcca
      721 tagcggaggc tcttggccgc agaccacagg agatcacggt cgccgtcctc gaccgcgaca
      781 ggcacgagac cctgatagag gagatccgaa agacgggagc gcgcatccag ataaggcccg
      841 agggcgacct caccccggcc atcgccgtag ggatgcgtgg ggccgacccg cacgccatca
      901 tcggtatagg aggcgcccct gagggcatcc tcgcagccgc ggtcatgaag tgcctcggcg
      961 gagagatgca ggccaggatg tggccgacgc aacgctcaca ggtcgagaag ctcgaagagt
     1021 acggctttac ggaccccgag cggaagctta ccctggacga ccttataagg ggcaccgacg
     1081 tactcttcgc agccacagga ataaccccgg gcgaacccct caacggggtc aggtatttcc
     1141 ggggcggtgc caggactcat acggtcgtca tgtcgaccga gcctcgtgtg gtaagattca
     1201 tggacactat ccatgcccta gagcccgagg caggccggcg gggcttccag ctttgacaag
     1261 caaaccagat gaaagacatt tccgcatcgt aaacaggaga ggtggagtac aaacatgacc
     1321 gaaagatcta cccttgagcg caagcgcctg agcgacctgc accagatcgc ctcgcagctg
     1381 ggaataaagc agtaccgcaa gtaccgtaag ccggagttgg ccgagatgat ctacaaggtc
     1441 gcgaccgagc aggcagccag ccagcagaac gagactacga ccaacggagg cgccacggcg
     1501 accgcaacgc gggaagcccc cgtaacgaca gacggcccca acggtgcgac ggagacccct
     1561 gtggcgcccg ttgacggctt cggcgaggcc gacacgaacc tcgccaccac cgaacaggtc
     1621 gagcgccagc gccaggagcg cggccgccgc gaccgtaaga acggccgtca ggatcgccgt
     1681 caggagcagc ggggcgacag ggacggcaag ggcggcgagg agaacgtgca gagcggaatc
     1741 ctcgatgtgc tgcccgacgg cttcggcttt ctcaggacgg ggggctacag ccagagcaag
     1801 ggcgacgtct acgtctcgac cagccagatc aaacgcttca acctgaggcg tggcgactac
     1861 atagttggcc agatccgtcc cgcccgcgag ggcgagaagt accctgccat ggtacggata
     1921 gagacggtga acgggcagga gcctcagaag ggccgctggc gccctaactt cgacgacttg
     1981 acgccgctgt atccgatgga gcggctgcgc ctggagtgga agccgaacga tatcgcccca
     2041 cgcgttatcg acctggtcgc acccatcggt aaaggccaac gcggcatggt cgtctcgccg
     2101 ccgaaggccg gcaagaccac gatcctcaag cagatagccc agtccatcgc ggccaactac
     2161 cccgagacca aactcttcgt ggtgctcgcc gacgagcgcc ccgaggaggt caccgactgg
     2221 cagcgaagcg tagaggaggc cgaggtcgtc gcctcgacct tcgaccagcc cgccgacaac
     2281 catatcgccg tcgccgaact cgtcctggag cgcgtcaagc gcctggtcga ggagggggag
     2341 gacgtagtcg tcttgctgga cggcataacc aggctctcgc gcgcctacaa cctcgccgct
     2401 cccgcttcgg gaaggatcct ctccggtggt gtggattcag cggccctata cccaccgaag
     2461 aagttcttcg gggcggcacg caacatcgag aacggcggtt ccctgaccat cctggcgacc
     2521 gcgctcgtcg agacggggag caggatggac gaggtcatct tcgaggagtt caagggcacg
     2581 ggcaacatgg agctcaggct cgacaggaag ctggcgaacc gcaggatcta cccggccata
     2641 aacatcgagg actcttcgac gcgcaaggag gaactgctca tggagccggc cgaggcccag
     2701 cgggtctggc aggtccgtag catcctcaat gccctggata acgcgcagaa gatcgagctc
     2761 ctgatccaga agctcaagga gacccgtacg aaccccgagt tcctgcgtga actccagcgc
     2821 gtgcgcggtt gacggctaga ctcggacggg tgcgttcaca caagacatgg cacccgtccc
     2881 tcgcctcctt tacagacgta atagactata tgagacggca tttgagatac ggaggtacat
     2941 gatgaagact ggtatacatc cagagtacac gaagacgctg gtgcgttgca tgtgcggcaa
     3001 cgagt
//
LOCUS       DASTKM010000173         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_222090, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000173 DASTKM010000000
VERSION     DASTKM010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222090"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="VFQ10_07160"
     CDS             complement(<1..1926)
                     /locus_tag="VFQ10_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HET9965156.1"
                     /translation="MSHRIRKVAVLGAGTMGAAIAAHCANAGLEVDLLDIAPDDDDDK
                     NAVVEAGFDRMKNARPAALMAENVADRIRIGNFEEHFGRVGEADWIVEAIIEKLEPKR
                     ELMQRVEDTAKEDAIISTNTSGIPLHSISEGRSEEFKRRFVGTHFFNPPRYLKLMEII
                     PTENTDPEIVEAVRNFGERVLGKGGVIAKDTPNFIGNRIGTFAGMQSARYAFENGYGI
                     EEVDAITGPLIGHPKTATFRLNDQVGLDIAVGVAVNLYEAVPEDESREELKPPEKLEK
                     MQEKNLLGNKTGAGFYKRDRRDGKTVFDVLDLETFEHHPAENPEIPIAKGAQEQGDLA
                     SRLQFLIGKADEDRHARYIRDTLLPYMAYASRRVPEISDTLEDVDHAMEWGFAHQTGP
                     FRTWDLLGVRETVENMESMDIEVASWVKEMLQAGNDTFYKTENGTELQFSPLSKEYEP
                     VRKDPMYVSLDRLRDEGKELARSDSASLLDLGDGVLCLEFHSKGNSIDAGTVEIGNRA
                     IQELERDDVVGLVIGNEGRNFSVGANLGELAYAVNNGDLDQIEKSVETLQDLLMAFRF
                     APKPVVSAPHGQTLGGGLEVCLHSDRIVAAGETYMGLVEVGVGLIPAGGGTKEMARRL
                     VSPPLHVAPDTPPLPFLQ"
     gene            complement(1941..2309)
                     /locus_tag="VFQ10_07165"
     CDS             complement(1941..2309)
                     /locus_tag="VFQ10_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET9965157.1"
                     /translation="MERTTPKDIFLSEVKTRDVFPLLILHIVREEPAYGNAIMAQIRE
                     ICAGTMSVSPNTIYPLLRRLEEKGYVQGEWERPDTRSRRFYKITASGEEKYAEIKGRF
                     EVHLLRVREAIESLRRELYG"
BASE COUNT          428 a          791 c          754 g          575 t
ORIGIN      
        1 ctggaggaag ggtagcgggg gggtatctgg ggcgacgtgg agcggcgggg agaccaggcg
       61 gcgggccatc tccttggtgc ctcctccggc cgggatcaag ccgaccccga cctcgacgag
      121 gcccatatac gtctcgccgg ctgctacgat gcggtctgag tggaggcaca cttcgagccc
      181 gccgcccagg gtctggccgt ggggggctga gaccacgggt ttgggggcga agcggaaggc
      241 catcaggagg tcttgcaagg tctcgacgct cttttcgatc tgatccaggt ctccattgtt
      301 gacggcgtag gccaactcgc cgaggttggc ccccacggag aagttgcggc cctcgttacc
      361 tatgacgagc ccgaccacgt catcacgctc caactcttgg atggccctgt tgcctatctc
      421 gaccgttccg gcgtcgatgg agttgccctt ggagtggaac tccaggcaca gcacgccgtc
      481 gccaaggtcg agcaggctcg ccgagtcgct cctggcaagc tctttgccct cgtctcgcag
      541 acggtccagg gagacgtaca tcgggtcctt gcggacgggc tcgtattcct tgctgagagg
      601 gctgaactgg agctcggtgc cgttctcggt cttgtagaaa gtgtcgttac ccgcctggag
      661 catctccttt acccaggagg cgacctcgat gtccatcgac tccatgttct cgacggtctc
      721 gcgcacgccg agcaggtccc aggtgcgaaa tggccctgtc tggtgggcga agccccactc
      781 catcgcgtgg tcaacgtcct ccagggtgtc ggagatctcg ggtacgcggc gcgaagcgta
      841 cgccatgtac ggcaacaggg tgtcccttat gtagcgggcg tgcctatctt cgtcggcctt
      901 cccaatcagg aactgcagcc tggaggcgag gtctccctgc tcctgcgccc ctttcgcaat
      961 ggggatctcg gggttctccg cagggtggtg ctcgaaagtt tctagatcca acacgtcgaa
     1021 gacggtcttg ccatcgcgtc tgtcccgctt gtagaatccg gcgccggtct tgttaccgag
     1081 caggttcttc tcctgcatct tctcgagctt ctccggcggc ttgagctcct cgcgcgactc
     1141 atcctcgggg acggcctcgt agaggttcac ggccacgccg acggcgatgt cgagcccgac
     1201 ctggtcgttg agtctgaagg tcgctgtttt cgggtggccg atgagcggcc ctgttatggc
     1261 gtcgacctct tcgatcccgt agccgttctc gaaggcatag cgcgcggatt gcatccctgc
     1321 gaaggttcct atccggttgc cgataaagtt cggggtgtcc ttggcgatga cgccaccctt
     1381 gccgagcacc cgttctccga aattacggac ggcctcgacg atctctgggt ccgtattctc
     1441 tgtgggtatg atctccatga gcttcaagta tcgcggcggg ttgaagaagt gcgtgcccac
     1501 gaagcgtctc ttgaactcct cggaccgtcc ctcggagatg ctgtgcagtg gtatgccaga
     1561 ggtattggtc gagatgattg cgtcttcttt cgccgtatct tcgacgcgct gcatgagctc
     1621 tctcttgggt tcgagcttct cgattatggc ctcgacgatc caatccgctt cgccaacgcg
     1681 cccgaagtgc tcttcgaagt tcccgatcct gatcctgtca gccacgttct cggccatgag
     1741 cgcggcgggc ctggcgttct tcatcctgtc gaagccggcc tctacgacgg cgttcttgtc
     1801 gtcatcgtca tccggggcta tgtcgagcag gtccacctcc aggcccgcat tagcgcagtg
     1861 ggccgcgatg gcggcgccca tcgtgccagc cccgagtacg gctaccttcc tgatcctgtg
     1921 cgacatcaag cctcccgatc ctatccgtag agttctctgc gcaaagactc gatagcctct
     1981 cttacccgca acaggtggac ctcgaatctg cccttgatct cagcgtactt ctcttcccca
     2041 ctcgctgtta tcttgtagaa gcgccggctc ctcgtatcag gccgctccca ctccccctga
     2101 acgtacccct tctcctcaag ccgcctgagc agggggtaga tcgtgttcgg cgagacgctc
     2161 atggttcccg cacagatctc acgtatctgt gccatgatgg cgttcccgta tgcaggctcc
     2221 tccctcacga tgtgcaaaat gagcagcgga aagacatccc gcgtcttgac ttcgctcagg
     2281 aagatgtcct tgggcgtcgt cctctccata gaccgccccg ccgatctttc caatgtcctc
     2341 gctaccctcc catcgaaaaa acttgtcaaa attgataata acacggataa taagtttgcg
     2401 tagagatctg ggtctcgata gagttgagat tcggagaggc caagcgaagg aggagtggag
     2461 tgatcgccga cgtcacccgg tcattgggga ctgactacta tctgctcgac gagctgttca
     2521 cggacgagga gcgtatgatc cgggacag
//
LOCUS       DASTKM010000174        10255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1103850, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000174 DASTKM010000000
VERSION     DASTKM010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10255
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103850"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1290)
                     /locus_tag="VFQ10_07170"
     CDS             complement(58..1290)
                     /locus_tag="VFQ10_07170"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET9965158.1"
                     /translation="MSVTEQKYFRKGFRMRENVQHLLDRDYRSEVVDRVRENGNVFET
                     DGLTIRLADEFGFCYGVDRAVDYAFQTREKFPDRRIFITGDMIHNASMNDRLREMGIE
                     FLSTGFEGPHEKAFDGLSPEDVVLLPAFGAPIDWVEKLKQKECVVVDTTCGSVLSVWK
                     RVTRYARRGFTSIIHGKYYHEETKATASQTLQDGGNGKYLILRNLKETGYVTDFILGR
                     IDAEELTERLAHGMCPGFDPEEDLQRVGVANQTTMLMSETMAVGEELRKAMAERYGAE
                     NIEEHFELFDTICSATQDRQDALFGLLESPLDLMVVIGGYNSSNTNNLAIIADERVPH
                     SYHIASGDCIDGDTIHHKPAGTPLDSREEVEESGWLPEGPVRIGLTAGASTPNSQIGL
                     AIGRILEARGLPPEIALE"
     gene            1419..1808
                     /locus_tag="VFQ10_07175"
     CDS             1419..1808
                     /locus_tag="VFQ10_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9965159.1"
                     /translation="MDRRATIMVADDDVEALGRSASALENAGFRVVSATDGRTALQEA
                     LTRRVDLIIMDVSIPQLSGVETCHCLKAMPKTQKIPVVLTAAKKDPAAKALAERTQGS
                     VRVLRKPFTDEDLISIVRQVARPKSLI"
     gene            1892..3415
                     /locus_tag="VFQ10_07180"
     CDS             1892..3415
                     /locus_tag="VFQ10_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET9965160.1"
                     /translation="MGLSNRRKALITLATLIGTFLAALDATVVGTAMPTVIGELGGLE
                     LYPWAFASYLLAATVTGPVFGKLSDTYGRKPVYLAGIFLFLLGSVLAGTSQSMAALIF
                     FRTIQGLGAGAVQPVAITIVGDIFELEARARIQGLFGAVWGISAVVGPAAGGFITDLI
                     SWRWVFYVNVPFGIIAAVLLAASLTESFERRERSMDYLGTALLTGGLVAVLLALLGGG
                     GAGELSVATLALLFGGLVVLALFVLVERRAEDPVVPLDLFSERIIAVSALGNVAMGGV
                     LLGVSVYVPLFVQGALGGSAITAGAAVASLSIGWPVGSFVGGRLLLLTGYRTTLLLGS
                     ALLALGSALCVPMDSGTSLSYVVLAVVVIGLGLGFSSTSYLVSVQNAVPWRRRGVATS
                     SVVFFRTVGGLIGVAVMGAFLNASLGDRYRAAVDRAAGGDPGLGHLLSDPNSLLQPAV
                     RSRIPEGAYSEVAGALAASLSPAFWVLLFFGLSALAVATFFPRGTAKELVSREEDYG"
     gene            3408..4061
                     /locus_tag="VFQ10_07185"
     CDS             3408..4061
                     /locus_tag="VFQ10_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965161.1"
                     /translation="MDETGCELTLDDLPSPPLFKLVDPEGRDVFQETEVGGEGAKVSA
                     LFSDRELAGEFSVGAAAHGMESLSGLDPRELSDWGSVEVFALSGADFVLVVSERGAAV
                     FHAGDVAQKAEEMAGEIPFPLYMISDERGEAPLITVEVDDGEVLVAALFSSPENARDF
                     RERASHLDLPDSLGTIDDRDGLRRHALVAREAGATYAVIDPASGLTEAIPIEELSVP"
     gene            4058..4645
                     /locus_tag="VFQ10_07190"
     CDS             4058..4645
                     /locus_tag="VFQ10_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965162.1"
                     /translation="MKPILCTDVDSTIWDTGAWVCSAVLDITGETLDMDRITTWTHVL
                     DTYGEQTTTAIFHRVFDPERVRDREPYAGASEVLRALQESQGIEIHFVTHNDPETLTP
                     HLEPWLKTHFGPDVGLTVTREDKLDILEQLGAFGLIDDRPDTIGRVADAGLWTAAKIQ
                     PWNRDLLTRRSDIHGFSHWQEVPDLLPTQYRTSIV"
     gene            4817..5050
                     /locus_tag="VFQ10_07195"
     CDS             4817..5050
                     /locus_tag="VFQ10_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965163.1"
                     /translation="MEGAESKEGMREDAPRIEGFLGRGSPVTVSARTGVDLEGYVCDQ
                     NESGLLLDARDSSGDPTGYEFLPWSSIERVSTG"
     gene            complement(5104..6243)
                     /locus_tag="VFQ10_07200"
     CDS             complement(5104..6243)
                     /locus_tag="VFQ10_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9965164.1"
                     /translation="MKLRSKRDLFEIPEDIVYLNCAYMSPQLRSAREVGEKAVSRKSR
                     PWEITPDDFFEDAEEIRALFARLVGGDADGVAIIPSVSYGISVAAANVPVREGEKIVI
                     LEDQFPSNVYAWGGLAEQSGASLVTLPRPEDLDWTRALLEEIDTDTAVVAVPNCHWTD
                     GSLVDLASVGESVREAGAALVVDAIQSLGAHPFDVSEVRPDFLVAASYKWLLGPYGVG
                     YMYVGEEYREGKPIEHNWINRRGSQDFSGLVDYQDAFQPGARRYDVGERSNFVLLPMA
                     AEALRQLIDWGVENVSETIGTLTDLVEERAGALGIVTIPKGRRARHMIGLMLGPDAPD
                     DLATRLTTHNVYVSVRGPSVRVSPHLYNTESDVYRLFDVLERVAG"
     gene            complement(6273..7682)
                     /locus_tag="VFQ10_07205"
     CDS             complement(6273..7682)
                     /locus_tag="VFQ10_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9965165.1"
                     /translation="MVVVGGGIGGLAAAALIGRAGHEVTLLEATPWLGGKSRRIELDG
                     RRMDTGPSLLTFPGVWEEFLRRWDAAEQEPGEAAEVAGLRLKRLPEVGRYYYRGETTS
                     LPVEEGHPWRAAWERFAGIHGGLGPEVTRLLTADPADRRTLPTLARLLRVYGAKLTTR
                     SYLDELQWLPEGLREIIAIHTLNAGVAPTRTPALYASMPAIMTVDGVFVPEGGVYEIV
                     RSLYRLARRAGVEVKTGEAVLEIGRGRVVTAEGEYRPERVVSDLDAGRLEILLEPERG
                     LAKERLSCSGVAVYATLREELPAWVGVHSVVLPSDPIALYTSLEAGIEPRESMAFLNY
                     YRGGEIYPNDGGTLALLLTAPANGRKYGLEDPFVRREIERVSVAAGLQEPATTYFGEY
                     EVLDPGYFGAWGSAGGALYGAMRPFWRSGPLHRPLYNDRRRPWLWRVGASVHPGGGIP
                     AVLGGAMISTGRMLRSLVK"
     gene            complement(7688..8530)
                     /locus_tag="VFQ10_07210"
     CDS             complement(7688..8530)
                     /locus_tag="VFQ10_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET9965166.1"
                     /translation="MILRLLYISRPLLWINTVGPATIGMWLAGNLFAWGALPILLWLT
                     LPFNLLIYGVNDVFDQETDAKNPRKGTLEGARIDRSEVRPLVLGVIFTNAPFMIYALL
                     FAPFSATAWMLLYALVFLGYSVPPARFKARPYLDSLSNAAYAFPLVFVPLSLGEGPVW
                     SAALGLMAWSAAKHTFDAVQDVDEDRRAGITTTAVRLGPDGVVLWGGALWALATACFA
                     FLSVPVALLNAAIAGTLLYVLRQSPTPETGHRLYKYSIAFPYVAGTFAGVQLVAALSL
                     GAYP"
     gene            8583..9329
                     /locus_tag="VFQ10_07215"
     CDS             8583..9329
                     /locus_tag="VFQ10_07215"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HET9965167.1"
                     /translation="MFTQLRRTTPRPLILACVLSLAAAFFAVRYPDVKGASLGSYVST
                     VLIALPSAVALFRYLGPRRATLSLLALSSLAYAVEAIGVATGFPYGAFYYGDALGPRL
                     AGIVPYLLPLSYAPLVIGAVAASWGTRLRLLHVLYATLLLVWMDAVLDPGAASLGFWV
                     WPQGGVYYGVPPSNYAGWLLSGALATALLLATGRWSETPRPTLLDSATIATAFWTGVA
                     FLSGLVAPALLGATLFAYLLGRRSHLHLVK"
     gene            9488..9709
                     /locus_tag="VFQ10_07220"
     CDS             9488..9709
                     /locus_tag="VFQ10_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965168.1"
                     /translation="MIRERATGGVGVRREDSAYRSGWEDGRFGQAQTFATNNSLAAWT
                     EYPDRLSYYRGHREGRRIREMLKSEGRAD"
     gene            complement(9962..>10255)
                     /locus_tag="VFQ10_07225"
     CDS             complement(9962..>10255)
                     /locus_tag="VFQ10_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965169.1"
                     /translation="ILAAIAIPNYIGQQDKAKDAAAMAQLRMAATSQQLYYVDRHAYA
                     GDTTDLEAYGFRQGAQPVTVGAADGSTYCMQAPGGAGTFRITQDTGRPVAGAC"
BASE COUNT         1841 a         3415 c         3069 g         1930 t
ORIGIN      
        1 gttcagcgaa aacaaaatgc tgaaatctca cgagcgaagg ccgagcgtgc tgaaatgcta
       61 ttcgagtgcg atctcgggcg gaagcccgcg agcctcgagg atgcggccga tggccagccc
      121 tatctgagag ttgggggtcg aggcgcctgc tgtgagacct atacgcacag gaccttcggg
      181 aagccagccg ctttcttcta cctcttccct gctgtccagc ggcgttcctg ctggtttgtg
      241 gtggatcgtg tcaccatcta tgcaatcgcc gctcgctatg tggtagctgt gcgggactct
      301 ctcgtccgcg atgatcgcca ggttgttcgt gttcgaggag ttgtagcctc caataacgac
      361 catcaggtcc agaggactct ccagtagccc gaagagggcg tcctgcctat cctgggtcgc
      421 cgagcagatg gtgtcgaaca gctcgaagtg ctcctcgatg ttctcggccc cgtatcgctc
      481 agccatcgcc ttcctgagct cctcgcctac ggccatcgtc tcgctcataa gcatcgtggt
      541 ctggttggcg acaccaacgc gctgcaggtc ctcctcggga tcgaagccag gacacatgcc
      601 gtgcgcgagc ctctctgtta gctcttcagc atctatcctg ccaaggatga aatcagtgac
      661 gtagcccgtc tccttcaagt tgcgcaagat caggtacttc ccgttgccgc cgtcctgcaa
      721 tgtctgcgag gcggtggcct tggtctcctc gtggtagtac ttgccgtgga tgatgctggt
      781 gaaaccgcgc ctggcgtagc gtgtaacgcg cttccacacc gagaggaccg acccgcaggt
      841 cgtatcgacc acgacgcact ccttctgctt tagtttctcg acccaatcta tgggtgcgcc
      901 gaacgcgggc aagagcacga cgtcctcggg ggagagtcca tcgaatgcct tctcatgggg
      961 cccctcgaag ccggtggaga ggaactctat acccatctca cgcaggcggt cgttcatgga
     1021 ggcgttgtgg atcatgtccc ctgtgatgaa gatgcgcctg tcgggaaact tctccctggt
     1081 ctggaaggcg tagtccacag ccctgtccac cccgtagcag aacccgaact catcggcgag
     1141 gcggatcgtg aggccatcgg tctcgaagac gttgccgttc tcacggaccc tgtcaacgac
     1201 ctcagagcga tagtcccggt ccagaagatg ctggacgttt tcccgcatcc tgaagccttt
     1261 acggaagtat ttctgctccg tcacgctcac ctctcgtacc cgacgttcgt tacccgtgct
     1321 cgaagctgag tgtagcgcat ctaccccgcc gcgtcggaga ggactgtggc gcaaatcacg
     1381 gtcgctgcaa atgtaaattg ggtgtaaaat ctatcgccgt ggaccgcaga gcaaccatca
     1441 tggtcgcaga cgacgacgta gaagccctgg gacgctccgc cagcgccctg gagaatgcgg
     1501 ggttccgcgt ggtctcggcc accgacggcc gcacggctct ccaggaggcc ctcacccgca
     1561 gggtagacct cataatcatg gacgtttcga tcccccagct gagcggcgtc gagacgtgtc
     1621 actgcttgaa ggctatgccc aagacccaga agatccccgt ggtcctcacc gcagccaaga
     1681 aagacccagc cgccaaagcg ctcgccgaac gcacgcaagg ctcggtgcgg gtactgagaa
     1741 agccctttac cgacgaagac ctcatctcca tcgtcagaca ggtcgcccgc cccaagtccc
     1801 tgatctaggg catctggttc acctcacgcg tcccggtgcg acgcggtggt aagatcgctc
     1861 cccgtaaaag ccgctaccgg agcactgttt cttgggactg agcaaccgcc gcaaggcgct
     1921 cataacgctc gccacgctaa tcgggacctt cctggccgcc ctcgacgcca cggtggtagg
     1981 gacggcgatg ccgaccgtga tcggggagct cggcggtctc gagctctacc cgtgggcctt
     2041 cgcctcttac ctgctcgcgg cgaccgtgac aggccccgtc ttcggcaaac tctccgatac
     2101 ttacggcaga aaacccgtct acctcgccgg gatcttcctc ttcctgctcg gcagcgtgct
     2161 ggcaggcacc tcacagagca tggcggccct catcttcttc aggacgatcc agggtctcgg
     2221 agcgggggct gtgcaacccg tcgccataac catagtaggg gacatcttcg agctcgaagc
     2281 gcgggcgcgc atccagggtc tcttcggggc ggtgtggggg atctctgctg tagtcggccc
     2341 cgcggcaggc ggcttcataa cggacctgat ctcctggcgc tgggtcttct acgtgaacgt
     2401 gcccttcggc atcatcgccg ccgtcctcct cgctgcgagc ctgaccgagt ccttcgagcg
     2461 ccgtgagcgc agcatggact acctcggcac agccctcctt acaggcggcc tcgtcgccgt
     2521 gttgctcgcg ctgctgggag gtgggggcgc cggggagctc tcggtggcaa ccctggccct
     2581 tctcttcggc gggttggtcg tgctcgcgct cttcgtactc gtcgagcggc gggctgaaga
     2641 ccccgtggtt ccgctcgacc tgttctcgga gcggatcatc gccgtctcgg ccctcggtaa
     2701 cgtggcgatg ggcggcgtct tgctcggcgt ctcggtctac gtgccgctct tcgtgcaggg
     2761 cgccctcggc gggagcgcga tcacggctgg cgccgccgta gcttcactct ccatcgggtg
     2821 gcccgttgga tctttcgtcg gcggaaggct gctcctgctc accgggtacc gcacgacgct
     2881 cctgctcggc tcggccctgc tcgcacttgg gtcggccctc tgcgtaccta tggatagcgg
     2941 aacctcgctt tcctacgtcg tactggccgt tgtggtgatc gggctggggc ttggcttctc
     3001 gagtaccagc tacctcgtca gcgtgcagaa cgccgttccc tggcggcgtc gcggcgtggc
     3061 cacctcctcg gtagtcttct tccgtaccgt cggaggcttg atcggggtcg ccgtgatggg
     3121 cgctttcctg aacgcttccc tcggagatcg ttacagggcc gccgtagaca gggcagcagg
     3181 gggtgatcct ggtctcgggc acctgctctc cgaccccaac tcccttctgc agccggccgt
     3241 tcgcagccgc atccctgagg gggcgtactc ggaggtggcc ggcgccctcg cagcctcgct
     3301 cagcccggca ttctgggtcc tgctcttctt cggtctctcg gccctcgccg tggccacgtt
     3361 cttccctcgc ggaacggcca aagaactcgt aagcagggag gaggattatg gatgagaccg
     3421 gatgcgaact gaccctggac gaccttccca gcccgcccct cttcaagctg gtcgatcccg
     3481 aaggaaggga cgtcttccag gagactgagg taggcggcga aggggcaaaa gtcagcgccc
     3541 tgttctcgga cagagagctc gcaggagaat tctccgttgg tgcggccgcg cacgggatgg
     3601 aaagcctctc tggcctggat ccaagggagc tctccgactg gggctcggta gaagtcttcg
     3661 cgctctctgg agcagacttc gtgctcgtcg tctccgaaag aggcgccgct gttttccacg
     3721 ccggagacgt ggcccagaag gctgaggaga tggccggtga gatccctttc ccgctgtaca
     3781 tgatctccga cgagagggga gaggcgcccc tgatcaccgt ggaggtcgac gatggagagg
     3841 tcctggtagc agcgctcttc agctctcccg agaacgcccg cgacttccgc gagcgcgcct
     3901 cccacctcga cctgccggac agcctcggta ccatagatga cagggacggc ctcagacgcc
     3961 acgccctcgt agctcgcgaa gcgggggcga cctacgctgt catagacccc gcatcaggcc
     4021 tcacagaggc aatcccgatc gaagagttgt cagtaccttg aaaccgatcc tctgcacaga
     4081 tgttgactcc accatttggg acacgggagc ctgggtctgt tcggcagtcc tcgacatcac
     4141 aggcgaaacg ctggatatgg accgtatcac cacctggacc cacgtcctcg acacctacgg
     4201 cgagcagaca acgaccgcca tcttccaccg cgtcttcgac cctgagcgcg tccgggatcg
     4261 ggaaccctat gctggcgcct cagaggtgct gcgcgcgctg caagaaagcc agggcatcga
     4321 gattcacttc gtaacccaca acgatcccga gaccttgacc cctcatctcg aaccctggct
     4381 caaaacccat ttcggccctg atgtgggact tacggttacg agggaggaca agctcgatat
     4441 cctggagcaa ctgggtgcct tcggcctcat agacgacaga cctgacacca tagggcgtgt
     4501 cgccgacgcc ggtctgtgga cggcggccaa gatacagccc tggaaccgcg acctcctcac
     4561 cagaaggagc gacatacacg gcttctccca ctggcaagaa gtcccagatc tcctccccac
     4621 acagtacagg accagcatcg tctagaggct atctgtcccg tcatcgaccc accacaaacg
     4681 aacgctgaca agccgaaatg ctgacaagcc gtctggcgaa gccaggcggc gtgctgacgt
     4741 gctgacaagc gcccgacagg gcgcgtgctg tactgtaacc ttccgtgtgt cagatacggt
     4801 acaagaagga gacacggtgg aaggtgctga gagcaaggag ggcatgcggg aagacgcgcc
     4861 gcggatcgag ggtttcttgg gccgtggcag cccggtcacg gtctcggcca gaaccggagt
     4921 cgatctggag ggctacgtct gcgaccagaa cgagagcggc ctgctcctcg acgcccgaga
     4981 ctcgagcggg gacccgaccg gatatgagtt ccttccttgg tcgagcatcg aacgggtgag
     5041 caccggatag cgtcggcgct cctcagaaca aggtagaagc gatggccgca cataccaacc
     5101 gctctaaccg gccactcgct ccaggacatc gaagagccga tacacgtccg attcggtatt
     5161 gtagaggtgg ggcgagacac gcacgctcgg gccgcgcacg ctgacgtaga cgttgtgggt
     5221 cgtaagccgg gtggcgaggt catcaggagc atcagggcct agcatgagcc cgatcatatg
     5281 ccgcgcccgc ctccctttgg ggatcgtcac gataccgagt gctccggccc tctcttctac
     5341 gaggtccgtc aaagtaccga tggtctctga gacgttctcg actccccagt cgatgagctg
     5401 gcgcaacgcc tcggccgcca tagggagcag gacaaagttg ctccgctccc ccacgtcgta
     5461 gcgtctggcg ccaggctgga aggcgtcctg gtaatctacc aaccccgaga agtcctggct
     5521 tccgcggcgg ttgatccagt tgtgctctat gggcttcccc tccctgtact cctccccgac
     5581 atacatatac ccgacaccat atggtccgag cagccacttg tacgatgcgg ccaccagaaa
     5641 gtcgggccga acctccgaga cgtcgaaggg gtgcgccccc agcgactgta tcgcgtcgac
     5701 gaccagcgcc gcccccgctt cgcggacact ctcgcccacg cttgcgaggt cgacgagcga
     5761 tccgtcggtc cagtggcagt tcgggacggc cacaacggca gtgtcagtgt cgatctcctc
     5821 gagcagggca cgggtccagt cgagatcttc tggacggggt aaggtgacga ggctggcccc
     5881 gctctgctcc gccagcccgc cccacgcgta gacgttggag ggaaactggt cctcgaggat
     5941 tacaatcttc tctccttctc gcacgggtac gttcgccgct gcgacggaga tcccatagct
     6001 gacggagggg atgatcgcga cgccgtcggc atccccgccg acgagccgcg cgaacagcgc
     6061 ccggatctct tctgcatcct caaagaagtc gtcaggcgtg atctcccagg gccgtgactt
     6121 gcgcgatacg gccttctccc cgacctcccg cgccgagcgt agctgcggcg acatgtacgc
     6181 gcaattcaga tagacgatgt cctctggtat ctcgaacagg tcacgtttgc ttctcaactt
     6241 catcccgaat gtcctcccat cctcggtctc tctcacttaa ccagagatcg gagcattctg
     6301 cccgtcgaga tcatggcccc cccgagaacg gccggtatgc cgcccccagg atgcaccgaa
     6361 gccccgaccc gccacagcca cggacgtcta cggtcgttgt aaaggggcct gtgcaatggt
     6421 ccgctcctcc agaacggacg catggccccg tatagcgccc cacccgcgct cccccaggcg
     6481 ccgaagtatc ctgggtcgag gacttcatac tccccgaaat acgtggtcgc cggttcttgc
     6541 agtccagcag cgacggagac gcgttcgatc tcccgcctga caaacgggtc ctccagaccg
     6601 tacttccgcc cgttcgccgg cgccgtgagc aataaagcca gcgtcccccc gtcgttgggg
     6661 tagatctcac ccccacgata gtagttgagg aacgccatcg actcccgcgg ctctatgcca
     6721 gcttcgaggc tcgtgtaaag cgcaatgggg tcggaaggca gcacgacgct gtgcaccccg
     6781 acccaggctg gcaactcttc cctgagcgtc gcgtacacgg ccaccccgga gcaagacagc
     6841 ctctccttcg cgagccctct ttcgggttcc agcaagatct ccagcctgcc ggcgtcgagg
     6901 tcgctaacca ccctctctgg cctgtactcg ccctccgccg tcacgaccct gccgcgcccg
     6961 atctcgagca cagcctcgcc tgttttgacc tccacccctg cgcgtcgcgc gagccggtac
     7021 aaagagcgca cgatctcgta gacgccgccc tcgggcacga aaacaccatc gacggtcatg
     7081 atcgcaggca tgctcgcgta gagcgcaggc gtccgcgtcg gcgcaacccc ggcgttgagc
     7141 gtgtggatgg cgatgatctc cctcaacccc tcaggcagcc actgaagctc atcgaggtag
     7201 gaacgggttg tcagttttgc cccgtagacc ctgagaagac gtgcgagcgt gggtagcgtc
     7261 ctgcggtctg cggggtcagc cgtcagtagc cgcgtgacct cgggcccgag tccaccgtgt
     7321 ataccggcga accgctccca cgctgcacgc cacggatggc cttcctccac aggcagcgac
     7381 gttgtctcgc cgcggtagta ataccgtcct acctcgggca gccgcttcag cctcagcccc
     7441 gcgacctcag ccgcctcacc gggctcctgc tccgcagcgt cccagcgccg cagaaactcc
     7501 tcccacacac cggggaaggt gaggagcgaa ggccctgtat ccatccgccg gccgtcgagc
     7561 tcgatgcgcc ggctcttccc gccgagccag ggagtggctt ccagcaacgt aacctcatgc
     7621 ccggcgcgtc cgatcagggc agccgcggcc agacccccga tgccaccgcc cactacgacc
     7681 acgcggctca agggtaagcc cccagagaca gcgccgcgac gagctgcacg ccggcgaagg
     7741 tgccggccac ataggggaag gcgatagagt acttgtagag ccggtgtccc gtctccggtg
     7801 tcgggctctg gcgcaggacg tacaacagcg tacccgctat cgcggcgttc agaagcgcga
     7861 cgggcacgct cagaaaggcg aagcatgcgg tggcgagcgc ccagagggca ccgccccaga
     7921 ggacgacgcc gtccgggccg agccggaccg cggtcgtcgt gatgccggcc ctgcggtcct
     7981 catccacgtc ctgcacagcg tcgaaggtgt gcttggcagc gctccacgcc atgagcccga
     8041 gcgctgcaga ccagacgggc ccttcgccga gggagagcgg cacgaagacg agcgggaagg
     8101 cgtatgccgc gttgctcagt gagtcgaggt atggacgggc cttgaagcgc gcgggtggga
     8161 cagagtatcc gaggaagacc agcgcgtaga gtagcatcca ggctgtggca gagaaaggcg
     8221 cgaacaggag agcgtagatc atgaacggcg cattcgtaaa gatgacgccg agcacgaggg
     8281 ggcggacctc ggagcggtct atccttgccc cttccagggt acccttgcgg gggtttttgg
     8341 catccgtctc ctggtcgaat acgtcgttga cgccgtagat gagaagattg aacgggagtg
     8401 tgagccacaa cagaatcggc agggctcccc acgcgaaaag gttcccggcg agccacatcc
     8461 cgatcgtggc cggacctacg gtgttgatcc agagcagggg ccgcgagatg tagagcagac
     8521 gcaggatcac agaccctaat atataccagc ggcatccccg cagaccctat aatatggcaa
     8581 gggtgttcac ccagcttcgc cgtaccacac cgcgacccct gatcctggcc tgcgtcctct
     8641 ccctcgcggc cgccttcttc gccgtccgct acccggacgt aaagggtgcg agcttaggct
     8701 catacgtctc taccgtcctg atcgccctgc cctcggccgt cgcacttttc cgctacctgg
     8761 gcccacgccg ggcaacactc tccttgctcg ccctctccag cctggcctat gctgtagagg
     8821 ccatcggcgt cgcgacaggc ttcccctacg gcgctttcta ctatggcgac gccctcggac
     8881 ccaggctcgc cggaatcgtc ccttacctcc tgcccctctc ctacgcgcca ctcgttatcg
     8941 gggccgtggc agcctcgtgg gggactcgac tacgtctcct gcacgtcctc tacgctaccc
     9001 tgctcctcgt ctggatggat gccgttctcg accctggcgc agcctccctg ggattctggg
     9061 tgtggcccca gggcggcgtc tattacggcg tgccacccag taactacgcc ggctggctgc
     9121 tctccggagc cctcgccact gccctgctcc tcgccaccgg aaggtggtcc gaaactccca
     9181 gacccaccct gctggatagc gcgaccatcg ccaccgcctt ctggacaggg gtagccttcc
     9241 tgtccggtct tgtcgcccca gccctgctcg gagcaacgct cttcgcatat cttctcggac
     9301 gccgctcgca tcttcacctg gtgaaatagc accaaatagc acaacctact ctcaattgac
     9361 gccccagagt caccaaatat ggcaatttta taacttttgt tcgtttttat taagatcagc
     9421 ccttacattt atgaatccat tgtgtttata tgatgctcgt aggagccact cgcctgggag
     9481 gtgtggaatg atccgagaga gagcaaccgg aggagtgggg gttcgccgcg aggattcggc
     9541 ctacaggagt ggctgggagg atgggcgctt cgggcaggcg cagacgttcg cgaccaacaa
     9601 cagcctcgct gcgtggaccg agtaccctga caggctttcc tactaccgcg gccaccgcga
     9661 aggcaggcgc ataagggaga tgctgaagag cgaaggccgc gctgactgat ccgcccgccg
     9721 ttacaggctg aacgattcta cctgcgactc ccgccgaacc cttatcctga ttccgcaacc
     9781 tcacagggca cgctgcgtat ccatgagcga gggccgacgg gcccccctcc aagtacgccg
     9841 tcggcccttg tcgtatgtta gcgaaagacg cgggcgagtt ccatttcgtt ccgtgttgtg
     9901 agagcaggcg gggctgcgtc tggcgtaaac tgaacgctct gcgccaacca ctggagatct
     9961 cctagcatgc gcctgctacg ggtctacccg tgtcctgggt gatcctgaac gtgcctgcgc
    10021 cgccaggggc ctgcatacag taggtacttc cgtcagcggc ccctaccgta accggttgcg
    10081 ctccctgcct gaaaccgtag gcttcgaggt ctgtggtgtc gccggcgtac gcgtgccggt
    10141 ccacgtaata gagttgctgc gatgtggccg ccatacgcag ttgcgccatg gcggcggcgt
    10201 ccttcgcctt gtcttgctgg ccgatgtaat tcggtatcgc gatggcggcg agtat
//
LOCUS       DASTKM010000175        12998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_222850, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000175 DASTKM010000000
VERSION     DASTKM010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222850"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1476)
                     /gene="pyk"
                     /locus_tag="VFQ10_07230"
     CDS             complement(52..1476)
                     /gene="pyk"
                     /locus_tag="VFQ10_07230"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET9965170.1"
                     /translation="MRRTKIVATLGPATSSEEAIGSLIRAGVDVTRFNFSHGEHSMHL
                     KNAEIVRGGARKLGRNVAILQDLQGPKIRTGEVEGETELVEGSRIVIAPGEFTGDASR
                     LSTSYESLADDVRPGHRLLIDDGLIGLRVESVEDGEVVCEVIEGGPVSSHKGLNFPDS
                     TLSIRGLTPKDVEDLRFGMEELHPDWVAMSFVRTADEVWEVKERIKEFGGNAPVISKI
                     EKHEAIDNIEEIIGASDGIMVARGDLAVELSAEWVPIEQKRMVARCRRLGKPVIVATQ
                     MLDSMIRNPRPTRAEVSDVANAIFDRADAVMLSGETAVGRYPMQSVQEMERICRASEG
                     AINYGRDIPASASWGRGDRYDAVTHAACELAEDLEAEAILTSTQTGLSCIRVARFRPP
                     NKILAISPVEHTVRMLALVWGVVSIHGEQAGSLEDRFKRAIEAAREAGHLNDGDEVIL
                     TGGTAGSVPGSTNVVEVSTVGEDI"
     gene            complement(1479..2780)
                     /gene="eno"
                     /locus_tag="VFQ10_07235"
     CDS             complement(1479..2780)
                     /gene="eno"
                     /locus_tag="VFQ10_07235"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9965171.1"
                     /translation="MTEIIAVHGREILDSRGNPTVEVELATVSGFVGRASVPSGASTG
                     QNEAVELRDGEGDRYGGKGVKNATENVDTELAQVVVGLDAADQRTLDLAMIEADGTDN
                     KGRLGANAILGVSLAAAKASAEATALPLYRYLGGAGAYTLPVPCANILNGGAHAANNV
                     DFQEFMVVPVGFDTYTEALHSVARVYQVLKKLLAERGLSGGIGDEGGFAPDLESNGAA
                     LGLISEAVERSGYSLGDQIAFALDPAASEFYENGEYDLAGEGKKLSREEMVDYYVGLC
                     DEYPILSIEDGLAEDDWEGWAMITERLGDRVQLVGDDLFVTNPKILARGIGEGVGNSI
                     LIKVNQIGTLTETFETMELAHKSGYTTMISHRSGETEDATISDLAVAVNAGQIKSGAP
                     ARGERVAKYNQLLRIEEALGSSAVYPGLAAFNPRVKPGRGD"
     gene            complement(2988..3956)
                     /locus_tag="VFQ10_07240"
     CDS             complement(2988..3956)
                     /locus_tag="VFQ10_07240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9965172.1"
                     /translation="MLVGFAILVLVGCAPAEPVANVDSQARKVVTFEGGDVTQGELDE
                     FAEQAGVPKDDPQYQATVQQIMPQIVSVEIAKAYAQEHNITVSDKEVDQELTKLKEQV
                     GDQARSSGQDVSNQEAYEQALKQNNITEEQLREDIRENLPVQKVQQRVAGDAEPSDEE
                     IQSYYEKNKEAQFMTPEQRCVRHILFNKDQKEKAEKVKQQLEDGGDFAKLAKEYSQDP
                     GSAANGGDLGCLGKGETVPEFEQAAFGAEQGEIVGPVQTEFGYHILEVTDVKPEQTRS
                     LQEVESQIRSQLATEKQSEKFNNWIEEQKEERDVKYLKGYKPPDQG"
     gene            complement(4072..7236)
                     /gene="mfd"
                     /locus_tag="VFQ10_07245"
     CDS             complement(4072..7236)
                     /gene="mfd"
                     /locus_tag="VFQ10_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET9965173.1"
                     /translation="MSERTKTRSLRAPRRVRAYLAANSERPALLLAASEEAAERYARE
                     AEVFTDGPVVHLPSRGAPYGDVFDPAVSRVGRRQRALSSLRSARVVVAGPLAFMERTP
                     LYEPMDLAAGTEIELDATLERLVVLGYERVDRISRPGEFAVRGGIVDVFPSTRRSPVR
                     VEWWGDEIESVRAVSLATQRVVRELEAVTVYAAREGDLAGLAATDEELPEEARRGVRV
                     PGLDRLLLGLNPVSPRTMLPEDVEIWREEPQEDVSEEIDGLVHELYDRGMPDPDVEFS
                     LGEEGETVSAPPVAAFADTLRAAARRLDALVEDGLAVFIACSSRGEARRTVYAFGEIH
                     RTMRETPRVDADLPPGLYALPGEVEEGFTYPEGGVAVFRRNSLLGRRREARRRTGGRS
                     LASFADLKVGDLIVHAMQGIGRFEGLVAQEVLGSTRDYMQVSYRGGDTLFVPYEQMEL
                     LHKYVGGDGARLDKLGGATWAQVTDRVRKRVKALAGELLRLHAARASAPGFAFPEDGE
                     WERELEESFPYQETPDQQSAITAVKSDMQMPHPMDRLVCGDVGFGKTEVAVRAAFKTA
                     LAGKQTMMLAPTTILVQQHERTFKVRLDSYAVRVESLSRFTLPADRRRILADFRAGEV
                     DVLVGTHALLGSEVRPRDLGFVVVDEEQRFGVRHKERIKEYKTSVDVLTLTATPIPRT
                     MQMGLASLRDISVIETPPAGRRSILTHVGPYDEDLVKHAIEREVARGGQTFFVHNRVE
                     SIDDVAERLRDLLPGVRFATAHGQMPERALEGVMQHFLDGQADVLVTTTIVESGLDVA
                     TANTLVVDRSDAMGLAQLYQLRGRIGRSTEQAYAYLFAPLGATVESQKRLEALMDFTE
                     LGSGFAIAMRDLEIRGAGNLLGAEQSGHIAAVGFEMYLTLLEEAVAAAKGEPQERREE
                     RPVIVELTLDAYLPPGYVMDEIERVDLYRRASGLGSLAEVEDLAEELVDRFGALPEPA
                     RNLLGLSRVKLLAREVGASSVTYRSDNLTVTGATAGSAAPALIRKATGGIVTTRTGRI
                     SVRAPELDPLTLAEESLRALARAG"
     gene            7333..7743
                     /locus_tag="VFQ10_07250"
     CDS             7333..7743
                     /locus_tag="VFQ10_07250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9965174.1"
                     /translation="MSTLDELNERGMGTLPGLIGLTILEAEEGKISSRLDLREELMAP
                     NGYLHAATVVAIADTSCGYGTFVNLPEGAQSFTTIELKCNFVGTKRGGAIGCEAKLVH
                     GGRTTQVWDATVSDEESGKPIALFRCTQMILYPR"
     gene            complement(7772..8656)
                     /locus_tag="VFQ10_07255"
     CDS             complement(7772..8656)
                     /locus_tag="VFQ10_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9965175.1"
                     /translation="MRPRDFAGLILLGALWGGSFLFIRVAVPALGPFLLVELRVGLAA
                     AALFLYALAVGSMPKIRNRWRSFLVLGFFNAAVPFSLISAAEIHLTASLAAILNSTTV
                     MFTAIVAAVWMDDALMARKVVGIVLGIVGVTVLVGWDPLRVNSLILLAVAAMLLASLS
                     YALGATYAKRSFSGIPPLGMAIGQLSGAGALLLPLAVVSVPEEAPSTVVALSMLGLAF
                     LSTAVAYLIYFRLIDNVGPTSTLTVTLLVPVFGLLFGVLLLDEPIGFGTLAGLGIILT
                     SVVLITGIAPRKTKPEGN"
     gene            complement(8717..9235)
                     /locus_tag="VFQ10_07260"
     CDS             complement(8717..9235)
                     /locus_tag="VFQ10_07260"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET9965176.1"
                     /translation="MNAYVDEIESPAGPLTFAVNEDGALMRLHFVESDYGPVMEEDLE
                     DEGYAVEADETRTAGVRKELSEYVSGRRRTFDVPLAFAGSVWQNAVWMELTHIPFGET
                     RSYGEIADNLGRPGAARAVGRANASNVLPVVVPCHRVIAADGTLGGFNGGLHLKERLL
                     EHERNVLAAAGH"
     gene            complement(9232..10725)
                     /locus_tag="VFQ10_07265"
     CDS             complement(9232..10725)
                     /locus_tag="VFQ10_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HET9965177.1"
                     /translation="MLKDFEHRYRAVRGRDVRFDGWFYVAVTSTGIYCRPSCPAATPK
                     RSNVRFYPTAAAAQYAGFRACKRCRPDAVPGSPEWDIRADIVARAMRLIADGVVDRDG
                     VAGLARRLCYTERHLHRLLFSELGAGPLALARARRAHTARLLIETTDLPITEVAFAAG
                     FASIRQFNDTIREVFAVTPRELRRGRGVHDASVRGEIPLRLPFRTPFDTNGMLHFLGT
                     RAVAGVETFDGETYRRTLRLPHGAGVVALSNGGDHVRCVLRLENLRDLGSAVQRCRSL
                     LDLDADPVAVTEVLGADPLLGALVRRSPGRRVPGSADGDELAFRAVLGQQVSVAGART
                     LAGRLVARCGVPLPEALAAVGDEPTHLFPGPHAVAGTVLEGLGVPKARRETLRTLARA
                     LAEGRIVLDPGSEREEVERRLLELRGIGPWTASYVAMRALGDPDAFLPSDLGVRKAIW
                     RLGNGSGRQSVAAVAERWRPWRAYATQHLWASLGGEKDQTKKGEVVA"
     gene            10922..11548
                     /locus_tag="VFQ10_07270"
     CDS             10922..11548
                     /locus_tag="VFQ10_07270"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET9965178.1"
                     /translation="MGGVLTDLVRWVIDVVHSLGYVGVFALILAGSVYLPVPTELTLP
                     LFGFLIGNGRLSFVPVLLTATAARVCAALILYAVGFRLREARVRRVIKHFERSKLLFV
                     SDFERASAVFERHGGKAILVGHLIPGVGALVSVPAGLKRMPLRWRFLGYTILGCSIWN
                     SSLIGLGWALGGRWRIVEVFTSFVGISALIVLIAAIIWFFWRRRKAHR"
     gene            complement(11635..12414)
                     /locus_tag="VFQ10_07275"
     CDS             complement(11635..12414)
                     /locus_tag="VFQ10_07275"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9965179.1"
                     /translation="MSKQETAGYTVGRVAALSGVTIRTLHHYDEVGLLSPGGRSAAGY
                     RIYEDRDLERLQRILFYRELGFTLDEILTIVDDPRTDTLGHLRRQRGLLNERIERLSA
                     MVDAIDYEMEAKTMDIPLTPEERLEVFGEFRPEDYAEEAEQRWGDTEAYKESQRRVSS
                     YKKEDWQELKAEEEEIRARLAAAFEAGLAPDSEEAMAAAEAHRRHISRWFYECDYEIH
                     RGLTEMYVSDERFRSNYDAQVPGLATFIRDAAHANADQHFS"
     gene            complement(12695..>12998)
                     /locus_tag="VFQ10_07280"
     CDS             complement(12695..>12998)
                     /locus_tag="VFQ10_07280"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET9965180.1"
                     /translation="EDLIVVHDDLDLEAGTVRVKVGGGAGGHNGLRSIIGRLGNDFVR
                     VRIGVGRPPAGVTVTDYVLSRMDPVVKDAIPKAADAVEFVLEHGPEAAMNRFNVRA"
BASE COUNT         2182 a         4295 c         4043 g         2478 t
ORIGIN      
        1 cagcggcctc agtctcctgg gtacgctcac ctggggctca ccatgctcct tctatatgtc
       61 ctcgcccacc gtggaaactt cgacgacgtt cgtcgagcct ggcacggacc ctgctgtccc
      121 gcccgtgagg atgacctcgt cgccgtcatt gaggtgtccg gcctccctgg cggcctcgat
      181 ggcccgcttg aaacggtcct ccagggagcc tgcctgttcc ccgtggatag agaccacgcc
      241 ccacaccagc gcgagcatcc tgaccgtgtg ctcgacgggg cttatggcca ggatcttgtt
      301 gggcggcctg aagcgtgcga cccttatgca agagagccca gtctgggtcg aggtcaggat
      361 ggcttctgcc tcgaggtctt ctgcgagctc gcaggccgcg tgggtaacgg cgtcgtagcg
      421 gtcacccctc ccccaggaag ccgaggccgg gatgtcacgt ccgtagttga tagccccttc
      481 cgaggcccgg cagatccgct ccatctcctg cacgctctgc atcgggtagc gtcccacggc
      541 cgtctcccct gaaagcatta ccgcgtcggc ccgatcgaag atggcgttgg cgacgtcgga
      601 gacctcggca cgcgtcggac gggggttccg gatcatggag tcgagcatct gggtggccac
      661 gataaccggc ttgccgaggc gcctgcaacg cgccaccatg cgtttctgct ctatggggac
      721 ccactcggcg gagagctcga ccgcgaggtc cccgcgggcg accatgatgc cgtccgaggc
      781 ccctattatt tcctcgatgt tgtctattgc ctcgtgcttc tcgatcttgg agataaccgg
      841 cgcgttgccg ccgaactcct ttatccgctc cttgacctcc cagacctcgt ccgccgttct
      901 cacaaaggac atagccaccc agtcggggtg gagctcctcc atcccgaaac gcaggtcctc
      961 gacgtccttg ggcgtgagac ctcttatgga gagggtcgag tcagggaagt tgagcccctt
     1021 gtgggaagag acagggccgc cttcaatgac ctcgcatacg acctcgccat cctcgacgct
     1081 ctcgacccgc agcccgatga ggccgtcgtc tatgaggaga cgatggccgg ggcgcacatc
     1141 gtcggccagg ctctcgtatg aggtcgagag ccggctcgcg tcccccgtga actctcctgg
     1201 ggcgatcacg atccggctgc cctctacgag ctccgtctcg ccctcgactt cgccggtccg
     1261 gatcttgggt ccctgaaggt cctgcaaaat ggcgacgttg cgcccaagct tcctggcgcc
     1321 gccgcgtacg atctcggcgt tcttcaggtg catgctgtgc tcgccgtggg agaagttgaa
     1381 cctcgtcacg tctaccccgg cacggatgag cgacccgatg gcctcctccg aggaggtcgc
     1441 cggaccaagg gtcgcgacta tcttcgtcct gcgcaacgtc agtcccctct ccccggcttc
     1501 accctcgggt tgaatgccgc caggccgggg tagacggccg aacttccaag agcctcctct
     1561 atgcggagga gctggttgta cttcgcgacg cgctcgccgc gggcaggcgc gccactcttg
     1621 atctgtccgg cgttcacggc cacggcgagg tcggagatcg tcgcgtcctc ggtctccccg
     1681 ctgcggtggc tgatcatcgt ggtgtaacct gacttgtgcg cgagctccat cgtctcgaag
     1741 gtctcggtga gcgtgccgat ctggttgacc tttatgagga tggagtttcc gacaccttca
     1801 ccgatgccgc gagcgagtat cttcgggttg gttacgaaga ggtcgtcgcc gacgagctgg
     1861 accctgtctc ccagcctctc ggtgatcatg gcccacccct cccagtcgtc ttcggcgagg
     1921 ccgtcctcga tgctcagtat aggatactcg tcgcaaagcc ccacgtagta gtccaccatc
     1981 tcctcccggg agagcttctt accctcgccg gcgaggtcgt actcgccatt ctcgtagaac
     2041 tcggaggcgg cagggtcgag cgcgaaggct atctgatccc ctaatgaata tccgctcctc
     2101 tcgacggcct ccgagatgag cccgagcgcc gctccgttgc tctcgaggtc gggcgcgaag
     2161 ccaccctcgt cgccgatgcc tccggagaga ccgcgctcgg cgagaagttt cttgagcacc
     2221 tggtaaaccc gcgccaccga gtgtagcgct tcggtgtagg tgtcgaagcc tacgggcacg
     2281 accatgaact cctggaagtc cacgttgttg gccgcgtggg cgccgccgtt caggatattg
     2341 gcgcaaggga caggaagggt gtaagccccg gccccaccga ggtaacgata cagcgggagc
     2401 gcagtggctt ccgcagatgc cttggccgcc gccagcgaga cgccgaggat cgcgttggcc
     2461 ccaaggcgac ccttgttatc tgtgccgtcc gcttcgatca tggcaaggtc gagggtcctc
     2521 tggtcggcag cgtcgaggcc gacgaccacc tgtgccagct cggtgtccac attctcggtg
     2581 gcgtttttca cgcccttgcc gccgtagcgg tccccttccc catcccgcag ttcgaccgcc
     2641 tcgttctggc cggtagaggc tcccgagggg accgaggccc ggccgacgaa cccgctcacc
     2701 gtggccagct ccacctcgac cgtcggattt cccctggagt ccaggatctc acgcccgtga
     2761 acagcgatga tttccgtcat tatcctccct ttgtcagcat ttcagcgttg tgtcggcgag
     2821 ccagcctccg agcctcaacg acgcccctgt acagtggtcg tggtacccga tttcttattg
     2881 tcctaccctg tgtgccgcac tcccatgttt ttggctgaca atccgccgca ggcggcgtgc
     2941 tgactagctc tatactaacc ccacgcccac gtgtggcgcc accgacccta cccctgatcc
     3001 ggcggcttgt agcctttcag atacttgacg tcgcgctcct ctttctgctc ctcgatccag
     3061 ttgttgaact tctcggactg cttctcggta gcgagctggg accttatctg cgactcgacc
     3121 tcctgaagtg agcgggtctg ctcgggcttg acgtccgtaa cctcgaggat gtggtagccg
     3181 aactcggtct ggacgggacc cacgatctca ccctgctcgg caccgaaggc agcctgctcg
     3241 aactcgggta cggtctcgcc cttgcccaag caaccgaggt ccccgccatt cgcggcgctg
     3301 cccgggtcct gggagtactc ctttgcgagc ttcgcgaagt ccccgccatc ctccagctgc
     3361 tgctttacct tctcggcctt ctctttctgg tctttgttga agaggatgtg cctgacgcac
     3421 cgctgttcgg gggtcatgaa ctgggcctct ttgttcttct cgtagtaact ctggatctct
     3481 tcgtcggaag gctccgcgtc gccggccacc ctctgctgta ctttctggac cgggaggttc
     3541 tcgcggatat cctcgcgcag ctgttcctcc gtaatgttgt tctgcttcaa ggcctgctcg
     3601 taggcctcct ggttgctcac gtcctgtccg gaggagcggg cctggtcgcc tacctgctct
     3661 ttgagcttcg tgagctcctg gtcgacctct ttgtcagaga ccgttatgtt gtgctcctgg
     3721 gcgtaggcct tggctatctc gacgctgacg atctggggca tgatctgctg tacggtggcc
     3781 tggtactgcg gatcgtcttt gggcacaccg gcctgctcgg cgaactcatc caactccccc
     3841 tgcgtgacgt ctccgccctc gaaggtcacc actttccttg cctgggagtc gacattggcg
     3901 accggctcgg ccggagcaca gcccaccaag acgagtatgg cgaagcctac tagcaaatag
     3961 acgcataaca tcctgtttcc gaaaaacatg ctcaagatga cgaagaacct cactgtttga
     4021 atgccagaga ccgccgggga ttatagcacg caggatggca gtttcggccc tttagcctgc
     4081 ccgcgcgagc gcccgcagcg actcttccgc cagggtgagg ggatctagtt cgggggcgcg
     4141 gaccgaaatc ctgccggtcc tcgtcgtcac gatccccccc gtggccttcc ttatgagggc
     4201 gggggcggct gagcccgccg tggcgccggt gacggtgagg ttgtctgaac ggtacgtgac
     4261 agagctcgcg ccaacctcgc gggcgaggag cttgacccgc gagaggccga gcaggtttct
     4321 ggccggctcg ggtagcgccc cgaagcggtc gacgagttcc tcggccaggt cttcaacctc
     4381 ggcgagggag ccgaggcctg aggccctgcg gtagaggtcc acgcgttcga tctcatccat
     4441 gacgtacccc ggcggcagat aagcgtcgag cgtgagctcc acgatgacag gccgctcctc
     4501 gcggcgctct tgcggctcgc cctttgcggc cgcgacggct tcttccagaa gggtgaggta
     4561 catctcgaag ccgacggccg ctatgtgccc cgattgttcg gcgccgagca ggtttcctgc
     4621 gccccggatc tccagatccc gcatggcgat ggcgaacccg ctccccagct cggtgaagtc
     4681 catcaatgct tcgagccgct tctgtgactc gaccgtcgct cccagagggg cgaacaagta
     4741 ggcgtatgcc tgctctgtgg agcgtccgat cctgccccga agctggtaca gttgggcgag
     4801 ccccatggcg tccgagcggt cgacaaccaa agtgttcgcc gtagccacgt cgagcccgct
     4861 ctcgacgatg gtggtagtga ccagaacatc ggcttgcccg tcgaggaagt gctgcatcac
     4921 cccctccagg gcccgctccg gcatctgccc gtgcgcggtt gcgaagcgca cgccgggcag
     4981 aaggtcgcgc agcctctctg cgacgtcgtc tatgctctcc acgcggttgt gtacgaagaa
     5041 ggtctgtccg ccgcgggcga cctcccgctc gatggcatgc ttgacgaggt cctcgtcgta
     5101 gggtccgacg tgcgtgagga tgctgcggcg tccagccggg ggcgtctcga tcacgcttat
     5161 gtctcgcagg gaggccaggc ccatctgcat ggtgcgtggg atgggggtcg ccgttagcgt
     5221 gagtacgtcc acagaggtct tgtactcctt gatgcgctct ttgtgcctga caccgaagcg
     5281 ctgctcctcg tccacgacga cgaacccgag atctctgggc ctgacctcgg atccgagcag
     5341 cgcgtgcgtc ccgacgagca cgtccacctc cccggcccgg aagtcagcca ggatcctgcg
     5401 ccggtcggcc ggcaaggtga agcgcgagag gctctcgacc ctgacggcgt aggagtcgag
     5461 cctcactttg aaggtccgtt cgtgctgctg cacgagaatg gtcgtcgggg cgagcatcat
     5521 ggtctgcttg cccgcgagcg ccgtcttgaa ggcggccctc acggccacct ccgtcttgcc
     5581 gaagccaacg tccccgcaga ccagacgatc catggggtga ggcatctgca tgtccgactt
     5641 gacggcggtg atagcggatt gctgatcggg ggtctcctgg taagggaaac tctcctcgag
     5701 ctccctctcc cactccccgt cctcggggaa agcgaagcca ggggccgagg ccctcgccgc
     5761 gtgcagccgc aagagctcgc cggcgagcgc cttgacccgc tttctgaccc tgtccgtaac
     5821 ctgcgcccac gtcgcgccac cgagcttgtc gaggcgcgcg ccgtcgccgc cgacgtactt
     5881 gtgtaacagc tccatctgct cgtatggcac gaagagcgtg tcgcccccac ggtagctcac
     5941 ctgcatgtag tcacgggttg agcctaggac ttcctgggcc accagcccct cgaagcgtcc
     6001 gataccctgc atggcgtgca cgatcaggtc cccgaccttc aggtcagcga aggaggcgag
     6061 cgacctgcca cccgtacgcc ttcgcgcctc tcgcctgcgg ccgagcagcg agttcctcct
     6121 gaagaccgca accccgcctt cgggataggt gaacccctcc tcgacctccc caggaagagc
     6181 gtagagacct ggtggcagat cggcgtcgac ccgcggggtc tcgcgcatgg tgcggtggat
     6241 ctccccgaaa gcgtaaaccg tccgcctcgc ctccccgcgt gaggagcacg cgatgaagac
     6301 agcgagccca tcctcgacca gcgcgtcgag tcttcgggcc gccgccctca aagtgtcggc
     6361 gaaagccgcg accggtggag ccgaaaccgt ctctccttct tcaccgaggg agaactcgac
     6421 gtcgggatca ggcatccccc tgtcgtagag ctcgtggaca agcccgtcta tttcttcgga
     6481 aacgtcttct tgcggctcct cacgccagat ctccacgtcc tcgggtagca tcgtgcgtgg
     6541 tgagacaggg ttcagcccga gcaggagccg gtcgaggccg ggtacccgca cgccgcggcg
     6601 ggcctcctcg ggcagttcct cgtccgtggc agcgaggcct gcgaggtcgc cttccctggc
     6661 agcgtagacc gttacggctt ctagctcgcg taccacgcgc tgggtggcca gagagacggc
     6721 gcggacgctc tctatctcat caccccacca ctccacacgc accggcgagc gccgggtgct
     6781 cggaaacacg tctacgatgc cgccacggac cgcgaactcc cccgggcggg agatcctgtc
     6841 tacccgctcg tagccgagga cgacgagccg ctccagcgta gcgtcgagct ctatctccgt
     6901 ccccgcggcc aggtccatcg gctcgtagag cggcgtccgt tccataaagg ccagcggtcc
     6961 ggccaccacg acgcgggccg aacgcagcga actgagcgcc ctctgccgcc gcccgaccct
     7021 gctcacggct gggtcgaaga catcaccgta tggggctccg cgggagggca ggtggacgac
     7081 aggaccgtcg gtgaagacct cggcctcgcg ggcgtaacgc tcggccgcct cctcgctcgc
     7141 ggcgagcagg agcgcgggac gctcgctgtt cgccgccagg taggctctga ccctgcgcgg
     7201 ggccctgagg gatctcgtct tcgtcctctc gctcaccagt agattctacc gcttgacggc
     7261 ggatctcaag caacgggatt cacctctggg ataatccgtc aagccgtgac aatcaccctg
     7321 gaggccccgt agatgtctac tctcgacgag ctgaacgaac gcggtatggg aaccctgccc
     7381 ggtctcatcg ggttgacgat actggaagcg gaggaaggca agatctcgag ccgcctcgat
     7441 ctccgtgagg agctcatggc cccgaacggc tacttgcacg cggcgacggt ggtcgccatt
     7501 gcggatacct cgtgcggcta cggaacgttc gtgaacctgc ccgaaggcgc acagagcttc
     7561 acgaccatag aactgaagtg caacttcgtc gggacgaaac gtgggggtgc tatcgggtgc
     7621 gaagcgaagc tcgtccacgg tggccgcacg acgcaggtat gggacgcaac cgtctccgac
     7681 gaggagagcg gcaagcccat agcgctcttt cgttgcacgc agatgatcct gtacccgcgc
     7741 tgaacattgt gcgaaccaac actgtatctc cctaattacc ttcgggctta gtctttcggg
     7801 gagcgatgcc cgtgatgagg acgacgctcg tgaggatgat cccaagcccg gccagcgtgc
     7861 caaacccgat gggctcgtcg aggagcagga cgccgaacaa cagcccgaag acaggcacga
     7921 gtagcgtgac ggtgagcgtg cttgtgggcc cgacgttgtc gatgagtcgg aagtagatca
     7981 ggtaagccac cgcagtcgac aggaacgcca gcccgagcat ggagagggcc acaacagtcg
     8041 aaggcgcttc ttcgggaacg ctaaccacgg ccaggggcaa gaggagcgcc cccgccccgc
     8101 tgagctgccc gatagccatc ccgagtggtg ggatgcccga aaacgaccgc ttggcgtagg
     8161 ttgctcccag cgcgtaagag agcgaagcaa gtagcatcgc cgccacggcc aacaggatga
     8221 ggctgttaac cctcaacgga tcccagccca cgagcacggt caccccgacg attccaagaa
     8281 cgatccccac gaccttcctg gccatcaggg catcgtccat ccacaccgct gcaactatcg
     8341 cggtgaacat tacggtggtc gagttcagga tggcggcgag ggaggccgtc aggtgtatct
     8401 cggcggcgga gatcagggag aaaggcacgg ccgcgttgaa gaaccccagg acgagaaacg
     8461 acctccaccg gttgcgaatc ttcggcatgc tgccaacggc cagagcgtag agaaacaggg
     8521 ccgcggcagc gagaccgact cgcaactcca cgagcaagaa aggacccaat gcaggcacgg
     8581 cgactcggat aaacaggaaa gagccgcccc acagcgcgcc gagcaggatg agaccagcga
     8641 aatctcgcgg tctcatgagg cgccctcttc acataagaca cgaacgttgt gttgccctct
     8701 cgccacgtcc gacaaactaa tggcccgccg cggcgagtac gttgcgctcg tgctcgagca
     8761 aacgctcctt gaggtgtagt ccaccgttga acccgccgag cgtgccgtcc gcagcgataa
     8821 cacggtggca gggtacgacc acgggcagga cgttggaggc gttggccctg ccgacggccc
     8881 tggccgcgcc tggccgtccg aggttgtccg cgatctcccc gtaggacctg gtctccccaa
     8941 aggggatgtg cgtcaactcc atccaaacgg cgttctgcca cactgagccg gcgaatgcga
     9001 gtggcacatc gaaggtcctg cgccgaccgc tgacgtactc ggacaactct tttcggacgc
     9061 cggcggttct ggtctcgtcc gcttctacag cgtacccttc gtcctccaaa tcttcttcca
     9121 tgaccggccc gtagtcgctc tctacgaagt gcaacctcat cagggctcca tcttcgttta
     9181 ccgcgaacgt gagcgggcct gcgggagact ctatctcgtc gacgtaagcg ttcacgctac
     9241 cacctctcct ttctttgtct gatccttctc gccaccaagg ctggcccaca agtgctgcgt
     9301 ggcataggcg cgccagggtc tccaccgttc ggcgacggcg gcgacgctct gccgaccgct
     9361 tccgttgccg agccgccaga tggcctttct gaccccgagg tccgagggaa gaaacgcgtc
     9421 ggggtcgccg agcgcccgca tcgcaacgta cgaagcggtc cacggaccta tcccccgcaa
     9481 ctccaacaat cgtcgttcga cctcctcacg ctcagagccc gggtcgagca cgatcctacc
     9541 ttctgcaagg gcccgcgcca gagtgcgcaa ggtttcacga cgcgccttgg gaacgccgag
     9601 accctcgagc accgtcccgg ctactgcgtg cggcccgggg aagagatgcg tgggttcgtc
     9661 gcctacggcg gcgagcgcct cggggagcgg tactccgcag cgggcgacga gccggccggc
     9721 gagggtgcgg gcgccggcca cggagacctg ctggccaagc accgcacgga atgcaagctc
     9781 gtcgccgtcc gcgctccctg gtacgcgccg gcccggcgaa cggcgaacga gcgcgccgag
     9841 tagcgggtcg gccccgagca cctcggtgac ggcgacaggg tcggcgtcga ggtcgagtag
     9901 cgagcggcat cgctgaactg cgctaccgag gtccctcaag ttctcgagcc tgaggacgca
     9961 ccggacgtgg tcgccgccgt tggagagggc cacgacaccg gcgccgtgcg gcagacgcag
    10021 cgtccgccgg taggtctccc catcgaacgt ctctacgccg gctacagcgc gagtgccgag
    10081 aaaatgcagc ataccgtttg tgtcgaacgg tgtcctgaac ggcagtcgga gtgggatctc
    10141 cccccgcact gacgcatcgt gcacgccccg accgcgccgc aactctctcg gcgttacggc
    10201 gaagacttcg cggatcgtat cgttaaactg gcgtatgctc gcgaatccgg cggcgaacgc
    10261 tacctcggtg atgggtagat ccgtggtctc tatgagcagc ctggcggtgt gggcgcgtcg
    10321 cgcgcgggcg agtgcgagcg gaccggcccc aagctcggaa aagagcaggc ggtgcagatg
    10381 gcgctccgtg tagcagagcc gccgcgcgag gccggcgaca ccgtccctgt cgacgacgcc
    10441 gtcagcgatg agccgcatcg cccttgccac gatgtcggcc ctgatgtccc attccggtga
    10501 gccaggcacg gcgtctggcc tgcaccgctt gcaggcgcgg aaaccggcgt actgcgcggc
    10561 ggccgcggtc ggatagaagc ggacgttgga ccgcttggga gtggcggcag gacaactcgg
    10621 ccggcagtag atccctgtag aggtcacagc gacgtagaac catccgtcga agcggacgtc
    10681 gcggccgcgt acggcgcgat agcgatgttc gaagtccttc agcacgctcc tattgtcgca
    10741 cccgataggc aagattactg gcggaaatcg gacatcggca tcgtacgagc catcacgcta
    10801 gccaagagtg ctttgtgggt tcgctgctcg cctggagtgc acgctttgag gcgtgtccgg
    10861 cgttgcatcg catggtgcat aattacgttg tcttggtgag gagataggag gatgaagcac
    10921 gatgggtggt gtgctcaccg atctcgtacg gtgggtcatc gacgtagtcc attcgctcgg
    10981 ttacgtcggc gtgttcgctc tgatattggc gggtagcgtg tacctaccgg tcccgacgga
    11041 gctgacgctg ccgctgttcg ggttcctgat cggcaacgga cgtctctcat tcgtccccgt
    11101 gctgctcacg gcgacggcgg ccagggtgtg tgctgcgctc atcctttacg ccgtcggatt
    11161 cagactcagg gaggcgcgcg tgcggcgtgt gatcaagcac ttcgaacgtt cgaagcttct
    11221 tttcgtctct gacttcgaaa gggcgagtgc ggtattcgag cgtcacgggg gaaaggcgat
    11281 cctggtcgga cacttgatcc ccggcgtcgg cgccctggtc tctgtgcccg ccggcctcaa
    11341 gcgcatgcca ctgcgctggc ggttcctcgg ctacacgatc ctgggatgca gcatctggaa
    11401 cagtagtttg atcggccttg ggtgggcgct cggcggccgg tggaggatag tagaggtctt
    11461 cacttctttc gtcgggattt cggccctcat tgttctcatc gccgctatca tctggttctt
    11521 ctggcgcagg cggaaggcac acaggtagac ggagccctgt gtgcgccggc gtcacggcca
    11581 ggaggctacg cgagctcgat gtcccagagg acctggcgat catcgaagtg ctgactagct
    11641 gaaatgctga tcagcattgg cgtgggccgc atccctgatg aaagtggcca ggccggggac
    11701 ttgcgcatcg tagttggacc ggaaccgctc gtcgctgacg tacatctccg tcaggccgcg
    11761 gtggatctcg tagtcgcact cgtagaacca gcggctgatg tgcctccggt gcgcctcggc
    11821 tgccgccatg gcctcctcgc tgtcgggtgc caggcctgcc tcgaaggccg cggccaggcg
    11881 ggcgcgtatc tcttcctcct cggccttgag ttcttgccag tcttccttct tgtagctcga
    11941 gacgcgccgc tgagactcct tgtaggcttc ggtatctccc cagcgctgct cggcctcctc
    12001 ggcgtaatcc tcgggacgga actccccgaa gacctcgagg cgctcctccg gtgtaagtgg
    12061 tatatccatg gtctttgcct ccatttcata gtcgatggcg tcgaccatcg cgctcaggcg
    12121 ctctattcgc tcgttcagca gcccacgctg gcgtcgcagg tgcccgagcg tatcggtgcg
    12181 cggatcgtca acgatggtca agatctcatc cagggtgaac ccgagctccc tgtagaacag
    12241 gatccgctgc agccgctcca gatcccggtc ctcgtagatc cggtagccgg ccgcactacg
    12301 tccgcccggc gatagcagcc cgacctcatc gtagtggtgc aacgtccgga tcgtaacacc
    12361 cgagagggca gccactctgc cgaccgtgta gcctgctgtc tcctgtttgc tcacggttca
    12421 cctcctttgc acagagaagt ctaaagcctc acgcaacgtg agggtcaagg gatttctcga
    12481 cgttttcgaa gttccgtgcc ggcgcctact attggattcg cgctttcgcg ggaatgacat
    12541 atgggggact ccggtgcgtc cgtccgaagt ccgaacgcgg tcctcttcaa acagactcgg
    12601 tatcagctct cagcaaacat aaggctgact ggctgacaag ctgaagtgcc gaccagcgtg
    12661 gtttttggaa gggttgccct tccagaaccc gctctcaggc gcgtacgttg aagcggttca
    12721 tcgcagcctc ggggccgtgc tcgaggacga actcgaccgc gtcggcggcc ttggggatgg
    12781 cgtcttttac gaccgggtcc atccgcgaga gcacgtagtc cgtgaccgtg actcctgcgg
    12841 gcgggcgccc gacaccaatg cgcacccgga cgaagtcatt gcccagtctc cctatgatgg
    12901 aacgcaggcc gttgtgcccg cccgcaccgc ctccgacctt gacgcgcacc gtccctgctt
    12961 ccaggtcgag gtcgtcgtgg acgacgatga ggtcttcg
//
LOCUS       DASTKM010000176        16681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1282241, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000176 DASTKM010000000
VERSION     DASTKM010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16681
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282241"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VFQ10_07285"
     CDS             <1..960
                     /locus_tag="VFQ10_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET9965181.1"
                     /translation="GGAGFIGSYLCERLLAEGYRVVCMDDLSTGSFENVAPFLAREAA
                     FEYVEHDVSTHIDVAGELDEVYHFASAASPADFARIPIEILKTGALGTHNSLGLARAR
                     GAHFMLASTSEVYGDPLVHPQHEDYRGNVSTTGIRSVYDEAKRYAEALTMAYHRHHRL
                     DTRIVRIFNTFGPRMREDDGRMVPNFMRQALQGEPLSVYGEGTQTRTVQYVDDLIEGV
                     IRLMKSDETRPVNIGNPVEYSVREVAGMIIELSGSPSEISHQPLPEDDPKQRCPDITR
                     AREVLGWEPQTQAPEGLAKTIEWFASVAEARSKGLPATRGESY"
     gene            962..1891
                     /locus_tag="VFQ10_07290"
     CDS             962..1891
                     /locus_tag="VFQ10_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9965182.1"
                     /translation="MRILLTGGAGFIGSYVAEYLLGRGHEVAVVDDLSTGRRENVPKG
                     AQFFKKDIRLGCDEIFRDFGPQALCHQAAQMDVRRSVREPDFDAEVNVLGTVRLLQNC
                     TEYGVEKVVFASTGGAVYGEQQVLPAPEDHPLFPVSPYGVSKLACERYMHYYHFEFGL
                     PYVALRYANVYGPGQDPHGEAGVVAIFCGNLARGRTSTINGGGNQTRDYVYVEDVARA
                     NVLALENEVPSGAYNIGTGVETSVNRLYDLLLEISGKELPPQYGPSKPGEQLRSCVDP
                     TLAAHTFDWRAQTVIHAGLEETLSSFAALEGSG"
     gene            complement(1987..3270)
                     /locus_tag="VFQ10_07295"
     CDS             complement(1987..3270)
                     /locus_tag="VFQ10_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965183.1"
                     /translation="MEIRAHPPGLQDVLEFPLVEALFGRRARRFSLGASIPDGPLAFT
                     SRHEPLPLTELEQMLVLTAAAGNTGWHYMIMRHAGYAPHLSNYSGAAGGRTFPSAAGF
                     HTSEVFFTDDEGVYLFETRDAPALVEQTTDRLPDLDTILDAHRDRIRKLSDDRLYIPA
                     EEPYMEGHNTWCANRPGSTLLIPVGDIAQHMIAVLCFLVQNGYGIYDDVNREQISALE
                     NFAHLVDLEEPLPLTFAEQYAITECTAELSTSCYAGMLMLQAMGLGGWMFDGIDRYTV
                     LGASDDPEVPGLGFRYDTDERWSLPNPTGLPGVFAGYCPPHYPDMRAAVEAFAERKFG
                     PGGPFHPDTPGPWKESTRVRSSAQVHSEEFKECVTVMAQYIFDRFGKFPGTVPSIFIL
                     TYLQAHHLELEFYDHHFGPGAYLQTHADHMARWHR"
     gene            complement(3395..5023)
                     /locus_tag="VFQ10_07300"
     CDS             complement(3395..5023)
                     /locus_tag="VFQ10_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET9965184.1"
                     /translation="MPAVLQPCDIGVIDGTKISSPCQPSSRPWIIAAASLGSGMAFLD
                     STILNVALPALQSDLGASVRAVQWTYGAYALVLSALLLVGGTLGDRFGRRRVFVLGAT
                     IFAVASVWCAVAPGTAQLITARGVQGVGGALLVPASLAIIGASFEGRLKAKAIGTWGA
                     LSAIAMAVGPVLGGWLVEEVSWRAAFLINPVLAAVAIPIALWHVPESRDPEAHNLDLV
                     GAVLATTGLAGLVYALIESSAWGLGAPRVLVALTVGLIGLASFIWVERRVKDPMVPPS
                     LFRSRTFDGANLVTLLFYMALTGSLYFLPFLMMQVHGYSALVAGSVFLPFVAMAFLIG
                     RLSGRISARFGTKIPLVVASLAVLVGLLLFAVPGVEHESYWTSFFPAMIVQGAGMALA
                     ITPLTTAALGSVDREHSGLASGVNNAVARVAGLMAVALLGVFVYGVFSMSLDARLHSM
                     DLSEEVRSEMEAAKTHLGAAEAPEGVNAGTEAHIERAIDESFVAGFRAVMLVSAGLAL
                     TSALVAALLVSDRSVGPSDGDSILHLQVGAGDRV"
     gene            complement(5334..6944)
                     /locus_tag="VFQ10_07305"
     CDS             complement(5334..6944)
                     /locus_tag="VFQ10_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HET9965185.1"
                     /translation="MASLVLRGQELRAEVAGSQFAPYGVRVVFDAAGIIDATCSCPYE
                     WGGLCKHIVAALLAYSHEPESVRELPELEETLSGLEREELKDLVLKLAERFPSLGEAI
                     EGEIALSSSSEPRQVNVDAIRRSLRASIQAQAYPEPYYDYWYTSGDLDEARRILDGAW
                     DLIRADDAPGALRVLEAITGECLEAPDALDREMMSDYGGEFLDFFEEVGSALTEALLS
                     VELTPEEREDYGAKLDVWMGELGDYRVGDTFGAAYLAIGQGWSYPPLVTVLEGEIPDD
                     AFFDELFGQPLIIARLNVLERRGRYEEYLRLSAAAGEDTRHAIMLARLDRSDEAVEYA
                     SKHLRTPEEALTVAEALREQGDAEAALLVGERGLSLDGRKVRLAGWVRDLAEGLGRRG
                     LALEAALAAFHADPSLASYVRVRELAGQAWPEHRERLLDHLRQSTSYLPMGKVDVFLH
                     ENLVGDAIAAVEGDPFGGLVARVTDAAIESHPEWVIETCRRQAEEIMNQGRSKDYDEA
                     VDWLEKVQDSHLTAGREEEWVALTSRSS"
     gene            complement(7129..8073)
                     /locus_tag="VFQ10_07310"
     CDS             complement(7129..8073)
                     /locus_tag="VFQ10_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9965186.1"
                     /translation="MGWKRGGYVSVLLVAFGASLWGTDAILRVPLLEVASPSQIVLLE
                     HLVLLLYSVPTVALGWRFFRGLGAAQWVALLVIGWGGSALATLLFTTAFAVGNPTVAI
                     LLQKTQPLFAVALAGILLRERLGWAYWPCFAVAVVGAYLISFGDLGPFRAISSAQALT
                     AALALGAALLWGSSTVLGRLVLKDMPFHALTGARLLLAVPLLAAIVAFQGSLGGIGAG
                     FAAEPGRVVLLALIPGLLALLLYYRGLSGTRASYATLAELAFPATAVVLNWAVLGVGV
                     TTNQVVGFVLLWGAVFVVGYLNAKAPAPDPTAPVVGAG"
     gene            complement(8339..9109)
                     /locus_tag="VFQ10_07315"
     CDS             complement(8339..9109)
                     /locus_tag="VFQ10_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HET9965187.1"
                     /translation="MTIRRLYPPPAREVSAIYEDLDLPPEHREGARPYVIINTVSSVD
                     GRTAMEGKAAGIGSTTDRRTMRTLRSKADAVMVGAGTLRAEKLSLGLDPTDGVVQPLA
                     IIVTDTGEVPLEEHLIVEGGQEVLILLSDAAPERVVERMRTLAPVMRVPADSTGGVDL
                     QKALLALEAENGVESLVAEGGPGLNHALISRQLVDELFLTVAPELLGGTADQTLTLLR
                     GPTLPTRDRPTLDLVSIHLSGAELFLRYSLARTSPGSA"
     gene            complement(9106..10299)
                     /gene="hflX"
                     /locus_tag="VFQ10_07320"
     CDS             complement(9106..10299)
                     /gene="hflX"
                     /locus_tag="VFQ10_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9965188.1"
                     /translation="MAELGRLASTLGIEVAGVLEQGRRDGTGYLGRGKREELRELVDE
                     TGARFVISDDELTASQARVLEKACGVSVVDRTELIIRIFEAHARDAASRLEVELADLE
                     YRLPRVKGKNPELSRLGGGGVTTRGPGEQQLEYDRRVIRNRMATINRKLQEEKAGRVV
                     RGARLREEGPPTVALVGYTNAGKTTILNALSGARRSTKDRLFETLETTTRLVEGSRKV
                     GPDGMWPDFVVTDTVGFIRKLPTQLVHSFASTLEVACDCDVTVVCADASSEELEEEIQ
                     TVTLTLSGAAENGIGSGPMVMCLNKMDLVSEARAAELRRDHPHAVTISAIEDCEALVE
                     EIYRAIASRRERMRVFIPHAEYAAVSRLYGLAEVHAQENTESGLWMDVSLPRSASGKY
                     APYRV"
     gene            complement(10344..11075)
                     /locus_tag="VFQ10_07325"
     CDS             complement(10344..11075)
                     /locus_tag="VFQ10_07325"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HET9965189.1"
                     /translation="MQVYRELPVLTNQARRRTAALVGIVSVTEEWTVARHKALAEEII
                     ASETKFVLDAGTGMYLNAILLDIPLAPKVSTELRRRAEEMAEGEPNPRRAARAKELEL
                     AGAGGRGSIWGGDLRYETTIVYVRPERHALDAAIDERSKRITMNGLEEAGRLRDMAAA
                     GTRISPQVMDSVGVRELYAHLSGTLSVQQAQDLVSTRTRQLARRQIRWFDKLARTLEG
                     RANINIVESTNQRAVLHNMHDIIDL"
     gene            complement(11224..12561)
                     /locus_tag="VFQ10_07330"
     CDS             complement(11224..12561)
                     /locus_tag="VFQ10_07330"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HET9965190.1"
                     /translation="MSSTTKTTAPIPLSSKPEGRASAGAKTACIRTFGCQMNVHDSDR
                     IRRMIQDAGYAEVQRYEDADLVILNTCYVRENAVDRVKGHLGELNRLRREGRVKRVAL
                     TGCIGAAEESAALRQTYEIDLVLGTHNTYELAEFVGLPTMRETYTPELPGTEGGHNAF
                     VTIMTGCNYQCSYCIVPTVRGRMICRPMGSVIGEVENLVAGGTGSITLLGQTVDAWRH
                     EGKRFCDLLEAVSESAPRVWFTTSHPSNMEDRTLKAIGERDTIVKRLHLPVQSGSDRM
                     LKVMHRGYKAERYRRKIEVFREAAPEGTLSTDIIVGHPGETEEDHEQTLGLVEDCAFD
                     SAYIFKFSPRQGTEAAELPGTVPDAVVQRRFLEVLRQVEQNAFTRNQRKVGGVEEIYI
                     RHGSTEDGKAIGETWSGHAVHVNTDAAPGDYVKARIESAGPHVLYAGEADTSA"
     gene            complement(12554..13477)
                     /locus_tag="VFQ10_07335"
     CDS             complement(12554..13477)
                     /locus_tag="VFQ10_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9965191.1"
                     /translation="MEGPYRDLGAGFARLTGVEGPRRAPSRITHVEDALLELARNARD
                     AGAAHIYVASTLKDRRFRTLTVIDDGHGIPKSHRDLIFEPGVTTRHLNPVTEPGDPST
                     HGAGLSLYQIKNHSVQTRVLSTSSPTSIQATFDTNSLPERTLQSSTRPSKSNLRATLE
                     RFAAITNAQSPRLQTYYGPPGRILATLLLHRIIQTHRDSAGLREAAFGLGLGVSVRSA
                     QRILRGEVRPVEGVSGGERGAGVRGRRAVGRRDGGAVLQLGEEESARIADILRRAARA
                     GYMEVEELRFESRPGEISLRARVYEPEEEYE"
     gene            complement(13491..15035)
                     /gene="rny"
                     /locus_tag="VFQ10_07340"
     CDS             complement(13491..15035)
                     /gene="rny"
                     /locus_tag="VFQ10_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET9965192.1"
                     /translation="MNVLLLVLGLVAGVAVGAALGYFVWKSRVDTRQAQARADARSIL
                     EDAEREAETKRREADLEAKESSLKIKDDAEAEVRSRRAEIARVEERLDNRDTALQRRE
                     GELDERRRRLTETEEAMRRKEEQLREREADHLRMLEEISGLTREEAERRLFSDLETEL
                     EGRLGRMVRDLTLEAEERADAEARRLISTTMERLASDLTSESTVKAVSLPSDDMKGRV
                     IGREGRNIRAFEAATGVDVIIDDTPETVVISSFDPVRREVARVSMERLVQDGRIHPGR
                     IEQIVAKARKDVEKEIKAAGRQALYDAKISGGMHNDLLRLLGALKYRTSYGQNVLAHS
                     VEVANLCGMMAQELGANVKIARRAALLHDVGKAIDHEVEGTHALIGGRFAKKSGESDD
                     VVRAISAHHHEIEMETVEDVLVATADAVSAARPGARRETTETYIERLRSLEDIALGHR
                     GVDKAYAIQAGREIRVMVQPSEVDDRIAAKLAYDISKQIESDLEYPGQIKVTVIRESR
                     VSEVAR"
     gene            complement(15095..15715)
                     /locus_tag="VFQ10_07345"
     CDS             complement(15095..15715)
                     /locus_tag="VFQ10_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HET9965193.1"
                     /translation="MPEITGVRERRGKARISVDGEYWAEIDAGVAIESGLREGAVLSS
                     EELHVARVAGERPIAMGRALNLLGYRARSEAEIRDRLTRYGYVGETIEGVVLRLQELG
                     YLDDAEFARLKAREKARRYGPRRVSVELEKSGVGDTLAREVVEEEFAGRSEVEEARSA
                     AARRYNGRGSDAQARRVYGFLVRRGYSAEVCAQVAREYREPPEAQG"
     gene            complement(15754..>16681)
                     /gene="recA"
                     /locus_tag="VFQ10_07350"
     CDS             complement(15754..>16681)
                     /gene="recA"
                     /locus_tag="VFQ10_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET9965194.1"
                     /translation="PTGALALDLALGVGGVPRGRVVEIFGPESSGKTTLALHIIAEAQ
                     KAGGLAAFIDAEHALDPTYAQAIGVDLENLYFSQPDNGEQALEIADTLVRSGALDVVV
                     IDSVAALVPRAEIEGEMGDSHVGLQARLMSQALRKLSGSLKRSGTTAVFINQLREKIG
                     VMFGSPETTPGGRALKFYSSVRLDIRRIGALKVGNDAVGNQTRVKVVKNKVAPPFKTV
                     EFDVMYGEGISREGSLLDVGIEHDVIQKSGAWFAYGDERLGQGRENAKQFLKENNEVR
                     ERILDEVYEKLGLLEPVETVDEVAEEPARQVL"
BASE COUNT         3001 a         5574 c         4996 g         3110 t
ORIGIN      
        1 ggaggggctg gcttcatcgg gagctacctc tgcgagaggc tcctcgccga gggctacagg
       61 gtcgtatgca tggacgatct aagcaccggc tcgttcgaga acgtggcccc tttcctggcg
      121 cgagaggctg ccttcgagta cgtagaacac gacgtcagca cccacataga cgttgccgga
      181 gagctcgacg aggtctacca cttcgcctcc gcagccagcc cggccgactt cgcgcgcatc
      241 ccgatcgaga tactcaagac cggcgcgctg ggtacccaca actccctcgg gctggcccgc
      301 gccaggggtg cccacttcat gctcgcctcc acctcggagg tctacggcga ccccctcgtg
      361 cacccgcagc acgaggacta caggggcaac gtgagcacca caggtatcag gagcgtctac
      421 gacgaggcca aacgttacgc cgaggccctg accatggcct accaccgaca ccaccgcctg
      481 gacaccagga tcgtgcgtat tttcaataca ttcggcccca gaatgaggga agacgatggc
      541 aggatggtgc ctaactttat gcgtcaggcc ctccaaggcg agcccctgag cgtctacgga
      601 gaaggcaccc agacaaggac cgtgcagtac gtcgacgacc tgatcgaagg tgtgatccgc
      661 ctgatgaaaa gcgatgagac ccgcccggtc aatattggca accccgtcga gtactccgtg
      721 cgcgaggtcg ccgggatgat catagagctc tcgggctcgc ccagcgagat ctcccaccag
      781 ccgctccccg aagacgatcc caaacaacgc tgccctgata taacccgcgc cagagaggta
      841 ctgggttggg agccacagac ccaggccccc gagggtctcg cgaagaccat cgagtggttc
      901 gcgtctgtgg cagaggctcg atcaaagggt ttgcccgcaa cacgcggcga aagctactga
      961 agtgcggatc cttctaaccg ggggagccgg gtttatcggc tcgtacgtgg cagaatatct
     1021 gctcggtcgc ggtcacgagg ttgccgtcgt cgacgacctc tcgacgggca ggcgcgagaa
     1081 cgtccccaag ggggcgcagt tctttaagaa agacatccgc ttggggtgtg acgagatctt
     1141 cagggacttc ggtccccagg cgctctgcca ccaggccgcc cagatggacg tgaggcgctc
     1201 ggtaagagaa ccagacttcg acgccgaagt caacgtcctc ggaactgttc gcctcctcca
     1261 aaactgcact gaatacggcg tggagaaggt cgtcttcgcc tcaactggcg gagctgtata
     1321 cggtgagcag caggtgcttc ccgcccccga ggaccacccg cttttccctg tgtccccata
     1381 cggggtctcc aagctggcgt gcgagcgcta catgcactat taccatttcg agtttggctt
     1441 gccctacgtc gcgctgcgct acgccaacgt ctacgggccg ggtcaggacc cacacggaga
     1501 agcaggggtc gtggcgatct tctgcggcaa cctggccagg ggcaggacct ccacgataaa
     1561 cggcggcggg aatcagaccc gcgactacgt atacgtcgaa gacgtagcac gcgcaaacgt
     1621 actggccctc gaaaacgaag tcccctctgg tgcctacaac atcggcaccg gcgtcgagac
     1681 tagcgtgaac cgtctctacg atctgctact cgagatctcc ggcaaggagc ttccaccgca
     1741 gtacgggccg tccaagccag gcgagcagct ccgaagctgc gtagacccca ccctcgccgc
     1801 acataccttc gactggcgcg cgcaaaccgt catccatgcg ggcctcgaag agaccctcag
     1861 ctccttcgct gcccttgagg gatctggcta gtccgccagc gaaagcaccg tccaggatca
     1921 gcaacccact cctctctgac ccagcatgcg ccaggctgat ataccgcagt cagggcacgc
     1981 tcgaccctat cgatgccagc gggccatgtg gtcggcgtgg gtttgcaggt aggcccccgg
     2041 gccgaagtga tggtcgtaga attccagctc caggtggtgg gcctgcaggt aggtcaggat
     2101 gaagatgctc gggacggtgc ctgggaactt cccgaagcgg tcgaagatat actgtgccat
     2161 gaccgttacg cactctttga actcctcgct gtggacctgt gcgctgctac gcaccctggt
     2221 gctctccttc cagggacccg gcgtatcggg atggaagggt cctcccggac cgaacttccg
     2281 ctcggcgaag gcttcgacgg cggcccgcat gtccggatag tgtggcgggc agtagcccgc
     2341 gaagacccct ggcaagcccg tagggttcgg cagcgaccag cgctcgtcgg tgtcgtagcg
     2401 gaaccccaac ccgggcacct cggggtcgtc gctggccccg agaaccgtgt agcggtcgat
     2461 gccatcgaac atccagcctc ccaggcccat tgcctgcagc atcagcatgc cagcgtagca
     2521 gctggtcgag agttcggcgg tgcactcggt gatggcatac tgttcggcga aagtcagcgg
     2581 caggggctcc tccaggtcca ctaggtgcgc gaaattctcg agcgcggaga tctgctcccg
     2641 gttgacgtcg tcgtagatcc cgtatccgtt ctggaccaag aagcagagga cggcgatcat
     2701 gtgctgggct atgtccccca ccggtatgag cagcgtgctc ccaggacggt tggcgcacca
     2761 ggtgttgtgc ccctccatat agggctcttc agcagggatg tatagcctat cgtcagatag
     2821 tttgcgtatc cggtcccggt gggcatccag tatcgtgtcg aggtcgggta gcctatcggt
     2881 tgtttgctcc accagcgcag gggcgtcgcg ggtctcgaag aggtagacac cctcgtcgtc
     2941 ggtgaagaac acttcgcttg tgtggaaacc agccgcagag gggaatgtgc gaccaccagc
     3001 ggctccggag tagttcgaga gatggggagc gtacccggcg tggcgcatga tcatgtagtg
     3061 ccagccagtg ttgcctgccg ctgctgtcaa caccagcatc tgctccaact cggtaagcgg
     3121 cagaggttcg tgtcgggagg tgaacgccag cggcccgtcg gggatcgaag ctcccagaga
     3181 gaaccggcgg gctcgccttc cgaacagcgc ttcgaccaac ggaaactcca ggacatcttg
     3241 caggccgggc gggtgtgctc tgatctccat gaaccttctc ctctcctggt agcccgactc
     3301 atcttgccat gggagaggcc gcaaagctac cgatgttact tgtggatcta tgctctcgtg
     3361 tcgcctctac ttctggggac gtggcatgtg gtgcctatac tctatcgccg gcgccaacct
     3421 gaaggtgaag gatcgaatct ccatccgagg gcccgacgct ccgatcactg accaaaagcg
     3481 ccgcgaccag agcgctggtg agcgcgagcc cggcagagac gagcatcacg gcgcggaacc
     3541 cggcgacgaa ggactcgtct atggcgcgct cgatgtgcgc ctccgtccct gcgttcacac
     3601 cctcaggggc ctccgccgcc ccgagatggg tcttcgccgc ctccatctcg ctgcgaacct
     3661 cctctgagag gtccatgcta tgcaggcggg cgtccaggct catggagaac accccgtaga
     3721 cgaacacgcc gagcagggcc acagccatga gtcccgccac ccgggcaaca gcgttgttca
     3781 cccccgaggc aaggcccgag tgctccctat cgaccgagcc gagcgccgcc gtagtaagcg
     3841 gggttattgc cagcgccatc cctgcgccct ggacgatcat cgcagggaag aacgaggtcc
     3901 agtaggactc gtgctccacc ccaggcacgg caaacagcaa gagacccacc aggacggcga
     3961 gtgaagccac caccagcggt atcttcgtcc cgaagcgggc actgatgcgc cccgacagac
     4021 gaccgatgag gaatgccatc gccacgaacg gcaagaacac gctgccggcg accaaggccg
     4081 agtatccgtg gacctgcatc atgaggaaag gcaggaagta caacgagccg gtcagggcca
     4141 tgtagaaaag tagcgtcacc aggttcgcgc cgtcgaaggt tctggaacgg aacagcgagg
     4201 gtggcaccat cggatccttg acgcgccgct cgacccatat gaaacttgca aggccgatca
     4261 gtcctacagt gagggcaacg agcaccctcg gggcgcccag gccccaggcc gaggactcga
     4321 tcagagcgta gaccaaaccg gcgagccctg tggtagcgag aacggcgccg accaggtcca
     4381 ggttgtgtgc ctcggggtcc cggctctcgg gcacgtgcca cagcgcgatg ggtattgcca
     4441 cggcggccag caccgggttg atcaggaaag cggcccgcca ggagacctcc tcgaccagcc
     4501 agccgcccag aaccggcccc acagccatag caatggcgct gagagccccc caggtcccga
     4561 tggccttcgc cttgagcctc ccctcgaacg aagccccgat gatcgccagc gacgcaggca
     4621 ccagcagggc ccctcctacg ccctgcacac cccgggcagt gatgagttgc gcggtaccgg
     4681 gggctacggc gcaccagacc gaggctacgg cgaagatcgt cgcgccaagg acgaagacgc
     4741 gccgtctccc gaagcggtct cccagagtcc ctcctacgag taacagtgcc gaaagcacca
     4801 gggcataagc tccgtaggtc cactgcaccg cccgcacgct ggccccaaga tccgactgca
     4861 aagcaggcag ggcaacgttc aatatcgtgc tgtcgaggaa agccataccc gagcccagag
     4921 aggctgccgc tataatccag gggcgtgagg aaggttggca cggcgacgag attttggtgc
     4981 cgtcaataac ccctatatcg caaggttgga ggacagcagg cattatcggg tcctccagaa
     5041 gcgactcttt ccacgtgatc tccgaaggtg cctgctcaga ggctcatgcc cgagtcccca
     5101 cgccaactca tcgtgatgtt gcatccacga ttcgatctcc ttcagggcgt gcatctccga
     5161 ctccccttgc gctctcgttg caatcacctg ctagaagtat agccgcgggc gaaggccgaa
     5221 gaatgatcgc tgccagggcg tttcggagaa gcgatgatct acagcgggtt gagcctggag
     5281 ctacttcaag tcctctagca tcggccgcag cttgtacttg cgctgttggt ggtctatgag
     5341 ctcctcgagg taagcgccac ccactcctcc tcacgcccgg cggtcagatg ggagtcttgc
     5401 accttctcca gccagtctac ggcctcgtcg tagtccttgg acctgccctg gttcatgatt
     5461 tcctcggcct gcctcctgca ggtctcgata acccactcgg gatgcgactc gatggccgcg
     5521 tccgttaccc tagcaacgag gcctccgaat ggatcccctt cgaccgcggc gatcgcgtct
     5581 ccgacgaggt tctcgtgcag gaagacgtcg accttgccca ttggcaaata ggaggtgctc
     5641 tggcgcaggt gatccaacaa ccgctcgcga tgctccggcc aggcctgacc ggcaagctcc
     5701 cgtacccgca cgtaggaggc gaggctcggg tccgcgtgga aggcggccag ggccgcttcg
     5761 agggcgagtc ctcggcgtcc aagaccttca gcaaggtccc tgacccagcc cgccagcctg
     5821 accttccggc catccagcga gagcccacgc tctccgacca gaagcgcagc ttcagcatca
     5881 ccctgctccc gcagcgcctc ggccacggtg agagcctcct ccggtgtcct caggtgcttc
     5941 gaggcgtact cgacggcctc atcggaccgg tcgagccggg cgagcatgat agcgtgtctc
     6001 gtgtcctcgc ccgcggccgc cgagaggcgc aggtactcct cgtagcggcc ccggcgttcg
     6061 agcacgttga gacgggcgat gatcagcggt tggccgaaga gctcatcgaa gaacgcgtcg
     6121 tcagggatct caccctccag aaccgtcaca agcggcgggt aggaccagcc ctgcccgatg
     6181 gccaggtagg ccgcgccaaa ggtatctcct acacggtagt cccctagctc gcccatccac
     6241 acgtcgagct tcgcgccgta gtcctccctc tcctcaggtg tgagctctac gctcaacagg
     6301 gcctcggtga gggccgatcc cacctcctcg aagaaatcga ggaactcgcc gccgtagtcg
     6361 ctcatcatct cccggtcgag cgcgtccggg gcctccaggc actctcctgt gatagcctcg
     6421 aggacccgca gggcaccggg ggcgtcgtcc gcccggatga ggtcccacgc accatcgagg
     6481 attcggcgtg cctcatccag gtctcctgaa gtgtaccagt agtcatagta gggctcagga
     6541 taggcctgag cctggatgct ggcgcggaga ctgcgccgga tggcgtccac gttgacctga
     6601 cgaggttcgg aggaggacga gagggcgatc tcaccctcga tagcctcgcc tagggaaggg
     6661 aagcgctctg ccaacttgag cacgagatcc ttcagctcct ccctctccag tccggagagg
     6721 gtctcttcga gctcgggcag ctcccgcacc gactcaggct cgtgggagta ggcgagcaag
     6781 gcggcgacga tgtgcttgca aaggccaccc cactcataag ggcaactgca agtggcatcg
     6841 atgattccgg ccgcatcgaa gaccacacga acgccgtagg gagcgaactg gctacctgcc
     6901 acctcggccc gaagttcctg gcctcgcaag acgagggagg ccacggcacc ctgccgatag
     6961 tagccctgac ctcgacgaaa tgattcagga gaggaaaatc tctcgattag ggcttcgcca
     7021 aaatcacgca actatctcac tccagtcctg ccactgatct tacaccgttg tgttccgtcc
     7081 tttagaaaac accaatcatg atgaaaacat accccgacga attggtccct atccggctcc
     7141 gaccaccggg gcagtcgggt ccggcgccgg cgccttggcg ttcaaatacc cgaccacgaa
     7201 cacggctccc catagcagta cgaagcccac tacctggttc gtggtcacgc cgacgccgag
     7261 gacggcccag ttgaggacga cggccgtggc cgggaaggcg agctccgcga gcgtcgcgta
     7321 ggaggcgcgg gtgcctgaga ggccccgata gtagaggagc agggcgagga gaccaggtat
     7381 cagggcgagg agcacgaccc gccccggctc ggcggcgaac ccggcgccta tcccacccag
     7441 ggagccctgg aaagctacga tggccgcgag caggggcacg gccagcagga gtcgcgctcc
     7501 ggtcagggcg tggaagggca tgtctttcaa gaccagccgt ccaagtacag tcgaggagcc
     7561 ccacaacagc gcggcgccaa gggcgagggc cgctgtgaga gcctgggctg agctaattgc
     7621 cctgaaaggc ccgaggtcgc cgaaggagat caggtaagcc ccaacgaccg ccacggcgaa
     7681 acacggccag taagcccatc ccaaccgctc gcgcagcaaa atccccgcca gcgcgactgc
     7741 gaacagcggc tgggtcttct gcaaaagtat cgccaccgtc ggatttccga cggcgaacgc
     7801 cgttgtaaag agcagcgtcg caagggccga gcctccccaa ccgatgacga gcagcgcgac
     7861 ccactgcgcg gcccccaagc cgcggaagaa cctccatccc agcgccacgg tcggcaccga
     7921 gtacagaagc agtacaaggt gctcgagcag cactatctgg gacggcgacg ccacctccag
     7981 aagcggcacc cgcaagatgg cgtccgtccc ccagagcgaa gccccaaagg cgacgagcaa
     8041 aacgcttacg taaccgcctc ttttccagcc cacgccgcta acccatctcg ggcccctgct
     8101 cagccttcgg tgacggcatc ccgccgttgg gccgctcctt caccacctgc tcatcggact
     8161 gccttcgaga ttcggcccag ctcaggtgaa gttggatcac gctcggcatc tgcgccgtgt
     8221 cttcgtcggt ctctccacag atccggtagt atgcatcgtt ccctcctgtg gactgcgtac
     8281 tctccgtccg attctacctg tgttcgtcgc cgccagccgt cttgcagaga tccgaaactt
     8341 acgcagagcc tggggaagtt cgggccaaag agtatcgcag gaagagctca gctcctgaga
     8401 ggtggataga caccaggtcg agcgtaggcc ggtcgcgagt cgggagcgtg ggacctcgga
     8461 gtaaggtgag ggtctggtcc gcggttccgc caagcagttc aggcgccact gtaaggaaga
     8521 gctcgtcgac gagctggcgc gagatcaggg cgtggttgag accgggtccg ccttcggcca
     8581 ccagggactc gacaccattc tcggcctcga gggccagtag ggctttctgg aggtcgaccc
     8641 cgccagtgga gtccgcaggc accctcatga ccggtgcgag cgtgcgcatc cgttcgacaa
     8701 ccctctcggg agccgcatcg gagagcagga tcaaaacctc ctgaccgccc tcgacgataa
     8761 ggtgttcctc cagcggcacc tcgcccgtgt cggtaacgat gatcgcgagc ggttgcacca
     8821 caccatccgt cggatcgagg ccgagtgaga gcttctcggc gcgcagcgta ccggcgccga
     8881 ccatcacggc gtcggctttg gaacggaggg tccgcatcgt ccggcggtcg gttgtgcttc
     8941 ctattccggc ggccttaccc tccatggccg tcctaccatc caccgagctg accgtgttga
     9001 tgatcacgta cggtcttgca ccctcccggt gctctggcgg aaggtccagg tcctcgtaga
     9061 tggcggagac ctcccgcgca ggaggagggt aaagcctgcg tatcgtcaca ccctgtaggg
     9121 agcgtacttg cccgaggccg agcgaggcag gctgacgtcc atccataagc ctgactcggt
     9181 gttctcctgg gcgtggacct ccgccaggcc gtaaaggcga ctcacggcag cgtattcggc
     9241 gtgggggata aagacccgca tcctctccct cctcgacgcg atggcgcggt agatctcctc
     9301 gaccagcgcc tcacagtcct ctatggcgct tatggtgacc gcatgcgggt gatccctgcg
     9361 caactcagcg gcgcgcgcct ctgaaacaag gtccatcttg ttgaggcaca ttaccatcgg
     9421 ccccgaacca atcccgttct ccgcagcccc ggagagggtg agcgtgaccg tctggatctc
     9481 ctcctccaac tcctcgctgg aggcgtccgc gcagacgacc gtgacatcac aatcacacgc
     9541 cacttcgagc gtcgaggcga aggagtgcac cagttgcgtg ggcagcttcc taatgaaccc
     9601 cacggtgtct gtgacgacga agtcgggcca cattccgtcg ggccctactt tcctgctccc
     9661 ctctacgaga cgggtggtag tctcgagcgt ctcgaagagc ctgtccttgg tcgatctcct
     9721 cgccccgctg agggcgttca ggatcgtagt cttgccggcg ttcgtgtagc cgaccaacgc
     9781 tacggtcggt ggtccctcct ctctcagccg cgcaccccgg acgaccctac ccgccttctc
     9841 ttcctggagc ttgcggttta tggtcgccat gcggttgcgg atgacgcgcc tgtcgtactc
     9901 cagctgttgc tctccagggc ccctagtggt tacccctccg ccacccaggc ggctcaactc
     9961 cgggttcttg cccttgaccc tgggcaaacg gtactccagg tcggcgagct cgacctcaag
    10021 gcgactcgcc gcatccctgg catgcgcctc gaagatccgg atgatcaact ccgtcctgtc
    10081 cacgacggaa acgccacacg ccttctccag taccctggcc tgggaagcgg tcaactcgtc
    10141 gtcggagatc acgaaacgcg cccccgtctc atctaccaac tcccgcaact cttcacgctt
    10201 gccacgcccg agatatccag tgccgtccct gcggccctgc tcgagtacac cggcgacctc
    10261 gatcccaagg gtactcgcca accgccccaa ctccgccatg tgtcgcatct ccccagcgcc
    10321 cgcgagtacc gctctctgct cattcataaa tcgattatat catgcatatt atgcaagacc
    10381 gctcgctggt tggtgctctc cactatgttt atattggcgc gtccttcgag tgtacgcgcc
    10441 agcttatcga accatctaat ctggcgtcgg gcgagctgtc ttgtcctggt tgagacgagg
    10501 tcctgtgcct gctgcactga gagcgtcccc gagaggtgcg cgtagagctc gcgcacccca
    10561 acggagtcca tcacctgcgg gcttatcctc gttcctgccg cggccatgtc tcttagtctt
    10621 ccggcctcct ccaggccgtt catggtaatt cttttcgacc tctcgtcgat ggcggcgtcg
    10681 agcgcgtgcc tctcgggacg gacgtagacg atggtggttt cgtagcgtag gtctcccccc
    10741 cagatagaac cccggccacc ggccccggcc agctctagct ctttggcccg cgcggccctg
    10801 cgggggttgg gttccccttc ggccatctcc tctgcccgcc ggcgtaactc agtggagacc
    10861 ttcggtgcga gggggatatc gagcaggatc gcattcagat acattcccgt tcccgcgtcg
    10921 aggacgaact tggtctctga tgcgatgatc tcctcggcta gcgccttgtg cctggctacc
    10981 gtccattcct cggtaaccga gacgatgcca acgagcgctg cagtacgcct gcgggcctgg
    11041 tttgttaaaa cgggtaactc tctgtagacc tgcatggaat ctacggccag cgtgggtacg
    11101 tacaggccct ctacctcggt gagcctctcg gcaagcgcgt cggagaggac gctcttgcca
    11161 cccgccgtag gcccgcaaac taccagagcg gtcttcttgc gattctgtgc caggcttcgt
    11221 ccctcaggca gaagtgtcag cttcgcccgc gtatagtacg tggggtcccg cggactctat
    11281 cctggctttc acgtagtctc ccggcgcggc gtctgtattg acgtgaacgg cgtgaccgct
    11341 ccacgtttcc ccgatagcct tgccatcctc cgtgctgccg tgacggatgt agatctcctc
    11401 tacgcccccg actttgcgct ggttccgcgt gaaggcgttc tgttcgacct gcctcagcac
    11461 ctcgaggaag cgccgctgga ctacggcatc gggcactgtt ccgggtagct ccgctgcctc
    11521 ggtaccctgc ctcggcgaga acttgaagat gtaagccgag tcgaaggcgc agtcctcgac
    11581 gagcccgagc gtctgttcgt ggtcctcctc ggtctcgccg ggatggccca cgataatgtc
    11641 cgtgctgagc gtgccctcag gcgccgcctc cctgaaaacc tcgatcttgc gcctgtacct
    11701 ctcggctttg tagccgcggt gcatgacctt cagcatcctg tccgaccctg actgcacggg
    11761 caaatgaagc cgcttgacga tggtatcccg ctccccgata gcctttagcg tgcggtcctc
    11821 catgttggag gggtggctcg tcgtaaacca tacccgcggc gcactctccg agacggcctc
    11881 caggagatcg cagaacctct tgccctcgtg ccgccacgcg tcgaccgtct ggccaagcag
    11941 cgttatggac ccagtcccgc cggcgacgag gttctctacc tccccgatca cactccccat
    12001 cgggcggcag atcatacgcc ccctcacggt agggactatg cagtaactgc actggtaatt
    12061 gcacccggtc ataatggtga cgaaggcatt atgcccgccc tcagtgccag gaagttcggg
    12121 cgtgtacgtc tcccgcatgg tcggtagccc tacgaactca gccagctcgt aagtgttatg
    12181 ggtgcccaac acgaggtcta tttcgtaggt ctgtcttagg gctgccgact cttcggcagc
    12241 gccgatgcaa cccgtgaggg ctaccctctt cacccggccc tcgcggcgaa gacggtttag
    12301 ctctccgagg tgccctttca cgcggtcgac ggcgttctcg cggacgtagc aggtgttcag
    12361 aataacgagg tccgcatcct cgtagcgctg gacctcggcg tagcctgcgt cctgaatcat
    12421 ccgccttata cgatctgagt cgtgcacgtt catctggcac ccaaaggtcc ttatgcaggc
    12481 ggtcttggct ccagcgctgg cacgcccttc aggcttgctg gagagcggta tgggcgccgt
    12541 cgtctttgtc gtgctactca tactcttcct ccggctcgta gactcgggct ctgagggaaa
    12601 tctctcccgg acggctctcg aacctcaact cctcgacctc catgtacccg gctcgcgccg
    12661 cccgcctgag tatatccgca atgcgcgcac tttcctcctc cccaagctgc agcacagcac
    12721 ccccatctcg cctcccaacc gccctacgac ccctgacccc cgcaccgcgc tcccctcccg
    12781 aaacgccctc cacaggccgc acctcacctc gcaatatcct ctgtgcgctc cttaccgata
    12841 cgccaagccc aagcccgaaa gccgcctccc gcaaacccgc gctgtctctg tgagtttgta
    12901 ttatgcggtg aagtaagagg gtggcgagga tacggcctgg tggtccgtag taggtttgaa
    12961 gccttgggct ttgggcgttg gttattgcgg caaagcgttc gagagtggcg cggaggttgc
    13021 ttttcgaggg tcgggtggag gactggaggg tacgttctgg gaggctattg gtatcgaagg
    13081 tggcctgtat ggaggtaggc gaggatgtgg agaggaccct ggtctgtaca gagtggtttt
    13141 tgatctggta gagggagagg cctgcgccgt gggtggaggg gtcgcctggt tctgtgacgg
    13201 ggttcagatg gcgggtggtg acgccgggtt cgaagatcaa atccctgtgg gactttggga
    13261 tgccgtggcc gtcgtcgatg acggtcaggg ttctgaagcg acggtctttg agggtcgatg
    13321 ccacgtagat gtgggctgcc ccggcgtcgc gggcgttgcg ggccaactcc aggagggcgt
    13381 cctccacgtg ggtgatgcgg gaaggggccc gccggggtcc ttccacgccg gtcaggcggg
    13441 cgaagccggc gccgaggtcc ctgtatgggc cttccactag cgtgcctctc ctacctggcc
    13501 acctcgctaa cccggctctc gcggataacc gtcaccttta tctggccagg atactcgagg
    13561 tcactctcta tttgcttgga gatgtcgtag gccagcttgg cggcgatacg gtcgtccact
    13621 tcggagggct ggaccattac ccttatctca cgtccggcct ggatcgcata ggctttgtct
    13681 acgcctctgt ggccgagggc gatgtcctcc agggagcgca ggcgctctat gtaggtctcc
    13741 gtcgtctcgc gcctggcgcc agggcgggcc gccgagaccg cgtctgcagt ggcgaccagc
    13801 acgtcctcca ccgtctccat ctcgatctcg tggtggtggg cgcttatggc gcgcaccacg
    13861 tcgtctgact caccgctctt ctttgcgaat cggcctccga tcaaggcgtg ggtgccctcg
    13921 acctcgtggt ctatggcctt cccgacatcg tgcagcaggg cggctctgcg ggcgatcttg
    13981 acgttggcac cgagctcctg ggccatcatg ccgcagaggt tggctacttc gaccgagtgg
    14041 gcgagcacgt tctggccgta ggatgtccgg tatttcagcg cgccgagaag ccgcagcagg
    14101 tcgttgtgca ttccgcccga gatcttggcg tcgtagagcg cctggcggcc tgcggcctta
    14161 atctccttct ccacgtcctt gcgggccttc gcgacgatct gctcgatgcg ccccgggtgg
    14221 atgcgcccgt cctgcacgag ccgttccatc gagacccgcg ccacctcgcg cctgacaggg
    14281 tcgaagctgg agatcaccac cgtctcgggc gtatcgtcga tgataacgtc tacgcccgtg
    14341 gcggcctcga aggcccttat gttcctaccc tcgcggccta ttaccctgcc cttcatgtcg
    14401 tcagatggca gagacacggc cttgaccgtc gattccgagg tcaggtccga ggcgagacgc
    14461 tccatagtgg tggagatcag acgccgcgcc tcggcgtcag cccgctcctc ggcctccagg
    14521 gtcagatcac gcaccatgcg ccccaaccgg ccctcgagtt ccgtctccag gtcggagaat
    14581 agcctcctct cggcctcctc acgggtcagg cccgagatct cctcgagcat cctgaggtgg
    14641 tcagcctccc tctccctcag ttgctcttct ttgcgccgca tcgcctcctc ggtctccgta
    14701 agcctgcgcc tgcgttcatc gagctcgcct tcacgcctct gcagggcagt atccctgtta
    14761 tccaggcgct cctcgacgcg tgcgatctca gcccgcctgg aacgtacttc ggcttcggcg
    14821 tcgtctttga tcttgagcga ggattctttg gcctcgaggt cggcctcgcg gcgtttggtc
    14881 tcggcctcgc gctcggcatc ctcgaggatg gaccttgcgt cggccctggc ctgggcctgg
    14941 cgggtatcta cacgtgactt ccacacgaaa tagcccaggg cagcgccgac ggcgacacca
    15001 gcgaccagtc ccaggaccag taacaaaaca ttcacaggtt ttccttccag cgggcctgcc
    15061 gcgagaacgg cggcccggca tcaagctagc tcgactatcc ctgggcctcc ggaggctccc
    15121 ggtactcccg ggcaacctgg gcacatacct ccgccgagta accccggcga accagaaagc
    15181 cgtagaccct acgcgcttgt gcgtcggacc cacgcccatt ataccgccga gcggcggcgg
    15241 atcgagcctc ctccacctcg gagcggcctg cgaactcctc ctcgactacc tcccgcgcca
    15301 gagtgtcccc aacaccactc ttctcgagct cgaccgagac ccgcctcggt ccgtacctgc
    15361 gcgccttctc gcgggccttt agacgcgcga actcggcgtc gtcgagatag ccgagctcct
    15421 gcagacggag gactactcct tctatggtct ccccgacgta tccgtaacgc gtgagccggt
    15481 cgcggatctc ggcctccgag cgggccctgt agcccaggag gttgagcgcg cggcccatcg
    15541 ctatgggcct ctccccggcc acccgcgcca catgcaactc ttccgacgag agaacggccc
    15601 cctcgcgcag gccgctctct atcgccacgc cggcgtcgat ctccgcccag tattccccgt
    15661 cgacagagat cctggccttt cctcgacgct cacggacacc agttatctcg ggcatgtata
    15721 cccccggagc agatgtgccg agaaacgggt tttttagagc acctgcctcg cgggctcctc
    15781 ggctacctca tcgaccgtct cgacgggctc gagtaggcct agcttctcgt agacctcgtc
    15841 gaggatacgc tcacgaacct cgttgttctc cttgaggaac tgcttggcgt tctcgcggcc
    15901 ctgcccgagg cgttcgtcgc cgtatgcgaa ccaggcgccg ctcttctgga tcacatcgtg
    15961 ctcgatgccc acgtcgagca ggcttccctc acgggagatc ccctctccgt acatcacgtc
    16021 gaactccacc gtcttgaaag gcggcgctac cttgttcttt acgaccttta ccctggtctg
    16081 gttgcccaca gcgtcgttgc cgaccttgag cgcgccgatg cgccggatgt ccagacgtac
    16141 gctggaatag aacttcagcg ccctccctcc cggcgtcgtc tcgggagagc caaacatgac
    16201 ccctatcttc tccctcagct ggttgatgaa aacggccgta gtcccggagc gtttgagaga
    16261 ccccgagagc ttccgcaaag cctggctcat gagccttgcc tgaagtccca catgagagtc
    16321 gcccatctct ccctcgatct cagccctagg tacgagggcc gccacagagt ctataacgac
    16381 gacgtcgagg gcgccggagc gcacgagagt atccgctatc tccagggcct gttcgccgtt
    16441 atccggctgg gaaaagtaga ggttctcgag gtcaaccccg atagcctggg cgtaggtcgg
    16501 gtcgagggcg tgctcggcgt cgatgaaggc ggcgaggcct cctgctttct gcgcctcggc
    16561 gatgatgtgc agggcgaggg tcgtcttgcc gctcgactct ggaccgaaga tctcgacgac
    16621 gcgaccccgt gggacgccgc caacgccgag ggcgaggtcg agggccaggg cgccggtcgg
    16681 a
//
LOCUS       DASTKM010000177         4967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_575876, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000177 DASTKM010000000
VERSION     DASTKM010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575876"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFQ10_07355"
     CDS             complement(<1..385)
                     /locus_tag="VFQ10_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9965195.1"
                     /translation="MRKRLLEDLLFFLDRPSVTGSEKRLCDDLEARISPSPEWEIERV
                     SNNLTVRRKEPDPSLPRVVLAGHLDTVPEPEGGLEVRIEGDRVYGRGASDMKAGDAMM
                     LALLESGAWDESRFDPVFVFYEQEEG"
     gene            complement(382..1212)
                     /locus_tag="VFQ10_07360"
     CDS             complement(382..1212)
                     /locus_tag="VFQ10_07360"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET9965196.1"
                     /translation="MSQSRRTGIKEKIEAAFEDRGLLEAEEYRTAVFDTIAALDSGEL
                     RVAQKRDGEWISNAWVMKAINLYFAVAEMHTIEAGPFEYHDKIPTKKDLAASGVRVVP
                     PGTVRYGAFVEPGVVVMPGYVNIGAYVGSGTMVDTWATVGSGAQIGRDVHLAGGVGIG
                     GVLEPPGAMPVVIEDRAFVGSRCIVVEGVRVEEGAVLGANVVLTGSTRIVDVTGPEEI
                     VYRGRVPARSVVVAGTRTKEFPAGSYQLQSALIIGRRRESTDQKTSLNEALREFGVSV
                     "
     gene            complement(1264..1656)
                     /locus_tag="VFQ10_07365"
     CDS             complement(1264..1656)
                     /locus_tag="VFQ10_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965197.1"
                     /translation="MAMRGTVAAEGNEALLGVEVLSQAGSSSLRVEAVVDTGFTGHLT
                     LPRATVDALGLPIIGSAESILADGSLVMEDVCLARVLWHGAERPVRVLVADATPLLGM
                     ALMRGSDLRVECVGGGEVSVERLEEPSD"
     gene            complement(1676..1939)
                     /locus_tag="VFQ10_07370"
     CDS             complement(1676..1939)
                     /locus_tag="VFQ10_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965198.1"
                     /translation="MRDRSYTTEEVVQLGREIYEREIRAQVEASHDGEFVVVDVTTGA
                     WEVDEDDVAASERVLAKNSDAVLYFARVGRRAAYRLGHRLDAR"
     gene            2244..3104
                     /locus_tag="VFQ10_07375"
     CDS             2244..3104
                     /locus_tag="VFQ10_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9965199.1"
                     /translation="MTFFALVLVLAAAVFHATWNLLAKRVGDGGAVFVWLFGLCSLVI
                     YAPLAVVVVLVSEPRLGAVQLLFMFGSGVLHLGYFVLLQRGYAVGDLSVVYPLARGTG
                     PLLATAAAIVLFGERPSLLVFIGIGLITAGVFVLTSESGSLSTGLGAGVVYGLLTGVF
                     IAAYTIWDKQAVSALLIPPLLQSWATILVMTLLLSPVAMTRRKEARALWRAHKPEVIG
                     VAVLSPLSYILVLMALVFTPVSYVAPAREISILIGAAMGARLLSEGDSTRRLIAAGAM
                     VVGIIALALG"
     gene            3382..4161
                     /locus_tag="VFQ10_07380"
     CDS             3382..4161
                     /locus_tag="VFQ10_07380"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HET9965200.1"
                     /translation="MAACREDTRVVGAFLGGSHAEGTADEYSDLDLYLIVDDQSYDTF
                     FAERRAFLGRLGEPVFFEDFSDFGFDMVVFIFSSGVEGELALGRASGFDHIHGGPFEV
                     LVDKEGILEGQVFPLHRPSQAEQRETLRWLIYWFWRDLSEFNRAMVRGRLWTAHGLLE
                     SLRLKCVDLARLKHEFFSTGTVGYERLEQAADAQDLEALRATFCPLEREAMLEAAGRL
                     VGFYLRTAPPLAIHQGIAYPAGLEAVVIDKLECWCALHLDR"
     gene            complement(4231..4620)
                     /locus_tag="VFQ10_07385"
     CDS             complement(4231..4620)
                     /locus_tag="VFQ10_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965201.1"
                     /translation="MSAPIHSHLTYDILPDRTQVVGRPDVLVVEGLNMLEAGLQEPSE
                     GHRVFVSDYCDFSIYVDAQERHIKEWYLERFLRLREEALGDSTAYFHRFAELSVEQAK
                     AEALRVWTRSTTPTSKRTSSRRKTGRA"
     gene            complement(4617..>4967)
                     /locus_tag="VFQ10_07390"
     CDS             complement(4617..>4967)
                     /locus_tag="VFQ10_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965202.1"
                     /translation="VYLPLSRLLYLHVSATRNLYRTAGVFLAGEEKEVPYVVGIAGSV
                     AAGKSTVARVLRALISRWPGSPEVELVSTDGFLYPNRVLRARGLMERKGFPESYDLPL
                     LLRFLADIKSARDG"
BASE COUNT          843 a         1593 c         1546 g          985 t
ORIGIN      
        1 gtccctcctc ctgctcgtag aaaacgaaga ccggatcgaa gcggctctca tcccacgccc
       61 cgctttccag cagcgcgagc atcatggcgt ccccagcctt catgtcggag gcgcccctgc
      121 cgtagaccct gtccccctcg atcctgacct cgaggccgcc ctcgggctcg ggtacggtgt
      181 cgaggtggcc tgcgaggacg accctgggaa gagaagggtc tggctctttg cggcggacgg
      241 tgaggttgtt agagacgcgc tctatctccc actcaggaga tggcgagatg cgggcctcca
      301 ggtcgtcgca gaggcgtttc tcagatcccg taacgctcgg acggtcgagg aagaagagga
      361 gatcttcgag gagtcgtttt ctcaaacgct tactccgaac tcgcgcaggg cttcgttgag
      421 agaggtcttc tggtccgtcg attccctgcg ccggccgatt atgagggcgc tctggagctg
      481 gtaggagccg gcggggaact ctttggtgcg ggtccccgcc acgaccacgg agcgtgcagg
      541 gacgcggccc ctgtagacga tctcctccgg accggtaacg tcgacgatcc tggtcgagcc
      601 cgtgagaacc acgttagccc cgagaacagc cccctcctcg acgcgcaccc cttcgacgac
      661 gatgcatcga gagccgacga aggcgcggtc ttcgatcacg acgggcatcg ctcctggagg
      721 ctcgagcacg cctcctattc ccacgccacc ggcgaggtgc acgtcgcgtc cgatctgggc
      781 ccccgagccg accgtggccc acgtatccac catcgtcccg ctcccgacgt aggccccgat
      841 gttgacatag ccaggcatga caacgacgcc aggctcgacg aaagccccgt agcgaacagt
      901 cccgggcggc acgacacgta cgccagaggc agccaggtcc ttcttggttg ggatcttgtc
      961 atggtactcg aaaggaccgg cctcgatggt gtgcatctcg gcgaccgcaa agtacaggtt
     1021 gatggctttc atcacccacg cgttagagat ccactcgcca tcacgcttct gtgcgacgcg
     1081 caactcgcca ctgtcaaggg ccgcgatagt gtcgaagacc gccgtgcggt actcctccgc
     1141 ttccagcagc cccctgtcct cgaacgcggc ctctatcttc tcctttatcc ccgtcctcct
     1201 actctgcgaa atagcaacac gtccgccagt ttagcaggag gccagacgtg cgcggcgcgg
     1261 tggttagtcg ctcggttcct ctagcctctc cacgctcacc tctccgccac ccacgcattc
     1321 gacgcgcagg tcgctgccgc gcatcagcgc catgcccaag aggggcgtcg cgtcggcaac
     1381 gagcacccgg accggccgct cggcgccatg ccagagcacg cgtgccaggc aaacgtcttc
     1441 catcaccagg ctgccgtcgg caaggatcga ctcggcgctt ccgataatcg gcagcccgag
     1501 ggcatcgacg gtcgcgcgtg ggagggtgag gtgccccgtg aacccggtgt ccaccaccgc
     1561 ctcgacccga agcgaggatg acccggcctg agaaaggact tctacgccca gcagcgcctc
     1621 attgccctcg gcggcgaccg tacccctcat cgccaaggcg ccaccgacga agactctagc
     1681 gggcgtccag acggtgcccg agccggtagg ctgctcgtcg tcctaccctg gcgaagtaga
     1741 gcacggcgtc cgaattcttg gcaaggaccc gctccgaggc ggcgacatcg tcctcgtcga
     1801 cctcccaggc tccggtggtg acatccacga ccacgaactc gccgtcgtgg gaagcctcta
     1861 cctgggcacg aatttcgcgc tcgtagatct cgcgcccgag ctggacaacc tcctccgtag
     1921 tgtaactgcg gtctctcata tgcttcattc tagcgtctca catgtaatct agtagatcgc
     1981 ttctggatga ggtgcggtgc gccttcagca gtggattccc gctttcgcgg gaatgacgta
     2041 cgggagggcc taccatgtgc gtccgcccgc agcccaaaga cgcctccgtt caaaccgacc
     2101 ggtatgaaat gctgactagc tgaagtgctg agagccgccg accggaggga ggcggcgtgc
     2161 tgacaagcga aggcgaagcc tgagcgtgct gaaatcctga ctagctgaaa tgctatgatc
     2221 gcgatgagag gaggatctgt ccgatgactt tcttcgcgct tgtgctggtt ctggcagcgg
     2281 ccgtctttca tgcgacctgg aacttgctgg ccaagcgggt gggggacggg ggtgctgtct
     2341 tcgtctggct cttcggtctc tgctcgcttg tgatctacgc ccccctcgcg gtcgtcgttg
     2401 tgctcgtgag cgaaccccgt ctgggggctg tgcaactcct tttcatgttc gggagcggtg
     2461 ttttgcatct cgggtatttc gtcttgttgc agcggggata cgccgtgggg gacctctcgg
     2521 tcgtctatcc tctggcccgc ggcaccgggc ccctgctcgc gaccgcggca gccatcgtgc
     2581 tgttcggtga gaggccttct ctgctcgtct tcatcgggat aggtctgatc acggccggcg
     2641 tcttcgtgct gacgagcgaa tcggggagtc tgagtacagg cctgggggcc ggcgtcgtct
     2701 acggtctttt gaccggcgtg tttatcgcag cgtacaccat ctgggacaag caggcggtca
     2761 gcgccctttt gatcccaccc ctcctgcaga gctgggccac tatcctcgtt atgacgctgc
     2821 tgctctcccc tgtcgcgatg acccgcagaa aggaggcccg cgccctgtgg cgcgcccaca
     2881 agcctgaggt aatcggggtg gccgtcctga gcccgctctc ctacatcctg gtcctcatgg
     2941 ccctcgtctt tacccctgta agctacgtgg cccccgcgcg ggagatctcc atcctcatcg
     3001 gggccgccat gggtgcgcga ttgctctccg agggagactc cacgcggcgc ctgatcgccg
     3061 ctggcgcgat ggtagtcggc atcatcgcgc tggcgctcgg ctaattagca agtcagcatg
     3121 ctcaggctta cgccttcgca tgtcagcttt tcgcagtcgg agacgaatct tgatgagtcg
     3181 atgctcctct gccattctct cgaacgcacg agcgtctcac cacactaccc ttagctgaca
     3241 tgctgaattg ctgacaagcg acagccggag gctggagcgt gttgacaagg cggcgcagcc
     3301 gctgtgctga caaactggag ttgctaagga aggggtagag tcttgaatac cgggaatatc
     3361 ggagtgacac tcgaccgttt cgtggctgcg tgtagagagg atacgcgcgt cgtgggcgcc
     3421 ttcctcggtg gttcgcacgc agagggaacg gctgacgagt attccgatct cgacctgtac
     3481 ctgatcgtgg acgatcagag ttacgacact ttcttcgctg agcggagggc atttcttggc
     3541 cgcctcggcg aaccagtctt cttcgaggac ttctcggact tcggcttcga catggtcgtc
     3601 ttcatcttct cgagcggcgt ggaaggggaa ctggcgctag gacgcgcctc cggattcgat
     3661 cacatccatg gcgggccttt cgaggtcctg gtggacaagg agggcatcct ggaggggcag
     3721 gtctttccct tgcaccggcc gtctcaggct gagcagaggg agacgctgcg gtggcttata
     3781 tactggttct ggcgcgacct ctcggagttc aacagggcga tggtacgcgg taggctctgg
     3841 acggcccatg gcctcctgga gagtctgcgg ctcaagtgcg tcgacttggc tcgtctgaag
     3901 cacgagttct tctcgacggg tacggtcggc tacgagaggc tggagcaggc cgcggacgcg
     3961 caggatctcg aggccctgcg cgcgaccttc tgtccgctgg agcgggaggc catgctcgaa
     4021 gccgctggtc gtctggttgg attctaccta cgcacagccc cgccactcgc catccatcag
     4081 gggatagctt accctgcggg tctcgaggcg gtggtcatcg acaaactgga gtgctggtgc
     4141 gccctgcacc tggaccgctg agcttccgtg cgcctcagat cttgcgcagg cgcacgctct
     4201 ggaccgagtg atcccgtacc ttctcgagga tcaggcgcgc cctgtctttc gtcggctcga
     4261 tgttctcttt gaggttgggg tggtcgatct cgtccagacg cgcagggcct cggctttggc
     4321 ctgctccacc gagagctcgg cgaaacggtg gaagtaagcc gtgctatccc cgagcgcctc
     4381 ttcccgcaga cgcaagaaac gctccagata ccattctttg atgtgcctct cctgcgcgtc
     4441 cacgtagatg gagaagtcgc agtagtcgga aacgaagacc ctgtgtcctt ccgagggctc
     4501 ctgtagtccg gcctccagca tgttgaggcc ttccacgaca aggacatcgg gacgccctac
     4561 gacctgtgtc ctatcgggga ggatgtcgta ggtcaggtgc gagtggatcg gggcgctcac
     4621 ccatcgcgag cggatttgat atctgcgagg aaccgcagaa ggagcggcag gtcgtagctc
     4681 tcggggaacc ccttgcgttc catcaacccc cgagcccgca acacgcggtt ggggtacaga
     4741 aagccgtcgg tgctcaccaa ctcgacctcg gggctcccag gccagcggga gatcagggcg
     4801 cgcagtaccc gggccaccgt gctcttgccg gcggcgacgc tgcctgcgat gccgacgaca
     4861 tacggcacct ccttctcctc cccggccaga aatacgccag ccgtccggta cagattccgc
     4921 gtggcgctca catggaggta caggagccgc gagagcggga ggtagac
//
LOCUS       DASTKM010000178         7833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1105807, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000178 DASTKM010000000
VERSION     DASTKM010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105807"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFQ10_07395"
     CDS             <1..324
                     /locus_tag="VFQ10_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9965203.1"
                     /translation="LEFTEGLAGHVLELMPSLAQVRLLRQWSGLCDMTPDFSPIMGVT
                     PVAGFLVDVGWGTYGFKAGPVSGETMAELVATGKTPELIASFGLDRFGRGDLMGEKGA
                     AAVGH"
     gene            334..660
                     /locus_tag="VFQ10_07400"
     CDS             334..660
                     /locus_tag="VFQ10_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HET9965204.1"
                     /translation="MILLPCPYCGPRNASEFRYVGEVSERPDPNEAGVDEWRTYLYAR
                     NNPAGWTTEMWYHSAGCRQHFVAERHTVTNEVRTSRLPKAPPRGEQEEQATGGNVAVD
                     GGGAIY"
     gene            657..3533
                     /locus_tag="VFQ10_07405"
     CDS             657..3533
                     /locus_tag="VFQ10_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9965205.1"
                     /translation="MTAQTVQKAVMPMRLDHRPGEVIDRGRELTFTWNGEEYPAYGGD
                     TIISALAACGERIFSRSFKYHRPRGLLTASFLDPGCMVQVDDEPNVRGAHRRVEPGMV
                     VSPQGVWPSLEYDVKSANRAFGRFLSAGFYYKTFIKPRRLWPLYRRMLRRFSAGGRVS
                     AGPAQGYFDKRYAHPDVLVAGGGPAGMAAAVAAGRAGAQVLLVEEEYELGGHLRWGNE
                     LDALRVLREEVSRTPGVEVMVNSVVNGRYDDNWVAVMQRDLSHVQERLVKARAKTLVV
                     APGLIERPYVFEGNDTPGVILSTAARRLINLYAVKPGDRAVVFSANESGDAAAEDLRR
                     SGVEVARVVDARRDGDILRAKGDGKGLRSVECADGAEIECDLLVTAAGWTAPTSLANM
                     AGDRPVYDERAARFFPSILPEDLLATGGIAGDGTLDELLAHARATGQEAARRAVRTAL
                     RLRAGIPQRADEQEEPESGSSPVAIPELPISAHPALFRGKTHGIVDYSEDVTSKDLVA
                     AEKEGYDSIELVKRYTTVTMGPTQGKLETVNAVAIMAEATGRTINETGTTVWRPPYSP
                     ITLGALAGRNFEPVRYSPMQPWHEIHGAKPLVAGAWIRPDHYGDPASEALNVRENVGI
                     IDVTPLGKFDLRGPDVPKLLDHLYINRWDNLEVGAVRYGVMCLEDGVVFDDGVTGCLG
                     PERYIMTTTSSGAAGVWEWIDHWLQTADPGWRIHVTPVTTAYASINVAGPKSRELLLR
                     LSDGLDLSPEAFPYMRVREGRVAGVEGCFMWRIGFTGELSYEVHVPAAYGLHVWETLM
                     QSGADLGVGPFGVEAQRILRLEKGHFIVGQDTDGLTGPYEVGLGRMVRLDKDDFAGKP
                     ELAWQSERDSRLRLVGLQPHDGSLVPPEASQIVENKNEIVGRITSSRMSPTLGRSICL
                     GFVAQHLTTPGTKVTVRLPDGERIPASVTPHHAHYDPEGTRQRG"
     gene            3526..4170
                     /locus_tag="VFQ10_07410"
     CDS             3526..4170
                     /locus_tag="VFQ10_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HET9965206.1"
                     /translation="MVEAAPIARSPIAQAAPTTIKHGWEVSARRSAADLKIIDCTPLA
                     KILVLASPDGEVARALGVPFGRAARDPHGTLVVGSGPGEWLLLATPGTSPAVTQRVDE
                     VQDEGLVSVFDATHGRAIMRITGAKAPDLLAKVCAIDLADEVTPNGAAFRSSVAKLVT
                     DVVRDDSGGSRSYLLHCERSSGQYLFDALIDAGDEFGIEVDGFVDPGKRGVHRV"
     gene            4167..5864
                     /locus_tag="VFQ10_07415"
     CDS             4167..5864
                     /locus_tag="VFQ10_07415"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET9965207.1"
                     /translation="MTLPKNLDVARQAKLLPLNEVAAEIGIGEHLLEPYGEGVAKIKL
                     DAIEELADRPKAKYVVISAITPTPLGEGKTTTTVGLGQAFKHIGKTATVAVRQASMGP
                     VFGIKGGAAGGGYSQVVPMETLNLHLTGDIHAVTEAHNQLAAMIDNHLYQGNALDLEP
                     HSITWRRVMDVNDRALRHIVAGLGARTDGVPRQTGFDITAASEVMAVLSLSTSLQDMR
                     SRFGRIVVGTDAEGSPVTAEDLKAAGVMTVIMKEAIKPNLMQTLENTPALVHAGPFGN
                     IATGNSSIVADEVGIRGGDYLVTEAGFGADMGAERFFNIKCRYSGLEPDAAVIVATVR
                     ALKAHSGRHEVKAGKPLPEEMQKENPDDVYGGAANLKKQIENIRLHGVSPVVAINAFP
                     TDHETEYDAIREIAGEMGARVAVCNHFSEGGAGAVELAEAVAEAAEEPTSFRFLYPDE
                     VSLREKIETIATKVYGAKDVEYNPQAARMLDSYETSGFGELPVCMAKTHLSITSDPSL
                     KGAPTGWTLPVREVRASVGAGFVYPICGDMRTMPGLSASPAAEGIDIDEDGEIVGLF"
     gene            5936..7270
                     /locus_tag="VFQ10_07420"
     CDS             5936..7270
                     /locus_tag="VFQ10_07420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9965208.1"
                     /translation="MQLPTRASYVVIGAGVHGLSTAWHLAKELKARGRGSGEDVIVLD
                     KTGVAAGASGIACGVIRNNYFQPAMRKLMAHSVSIWESDPEAFAYHPVGYMQISPEVM
                     HHDVVQIYKEQQAIGYPSTLVEGEEECRSYMQNIFHDWQAENITSILHEHRGGYANNK
                     ASVRTLAAKAQAEGVRIHTGVRVTGFSMEDERVRSVQTDWGAVGCEHLVVAVGPWVRD
                     IWAMLDLPEKITVKGPDGESYRDREMWTYWSLREGVLGVEPSFLTDNEGRMPPVIHVD
                     TDAPLYDEDGELVTDELWGIYYKPDFYFNGVQGGATPYVVEGPASEVAVDPYGPESPD
                     FVVGEDFARMWTSALAHCHGRFEGKGYLFSREPSGGIGCFTPDSFPVFDTFRDNAYVI
                     ADSNHGYKMIGVGALVAKELMDEPQEQLEPFRFSRYETGELHPTSNSPFPWS"
     gene            7261..>7833
                     /gene="folE"
                     /locus_tag="VFQ10_07425"
     CDS             7261..>7833
                     /gene="folE"
                     /locus_tag="VFQ10_07425"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET9965209.1"
                     /translation="MELTQGDSEDLRSSSSSKYRVDRLRIECAVRELLIAIGEDPERE
                     GLRGTPHRVAEAYASLFAGLADDPARHLDMGFQEEHREMVLVRDIPLHSLCEHHLMPF
                     VGKAHVGYIPDGKVVGLSKLARLVEGYARRPQLQERLTAQVADAIHERLGARGAIFVV
                     EAEHLCMTMRGVQKPGSVTVTSAVRGIYARD"
BASE COUNT         1488 a         2515 c         2586 g         1244 t
ORIGIN      
        1 ctggagttca ccgagggcct ggccgggcac gtgctcgagc tcatgccctc cctggcccaa
       61 gtgcgcctgc tgcgccagtg gtcagggctg tgcgacatga cgcccgactt ctcgcccatc
      121 atgggcgtaa cgccggtggc cggcttcctc gtagacgtgg gctggggcac gtacgggttc
      181 aaggctggcc cagtttcggg cgagactatg gccgagctcg tcgccacggg caagacgcca
      241 gagctcatcg cctccttcgg cctcgaccgg ttcgggagag gcgatctgat gggagagaag
      301 ggagcggcgg ccgttgggca ctgagggaag gccgtgatcc tgctgccgtg cccctactgc
      361 gggccacgca acgcttcgga gttccgctac gtcggcgagg tctcggaacg ccccgacccg
      421 aacgaggctg gcgtcgatga gtggaggact tacctctacg cgcgcaacaa cccggcgggc
      481 tggaccacgg agatgtggta ccacagcgca gggtgcaggc agcatttcgt cgccgagcgg
      541 catacagtga ccaacgaagt gagaacctcg cggctcccca aagctccccc gcgcggcgag
      601 caagaagaac aggccaccgg cgggaacgtc gcggtcgacg gtggaggtgc gatctattga
      661 cggcgcagac ggtacagaag gccgtcatgc ccatgcgcct ggaccaccga ccgggcgagg
      721 ttatcgaccg cggacgagag ctcacgttca cgtggaacgg cgaggagtac cccgcttatg
      781 ggggggacac aatcatctcg gcactcgcgg cttgcgggga gcggatcttc tctcgcagct
      841 tcaagtacca ccgtccgcgc gggctgctaa cggcgagctt cctggacccg ggttgcatgg
      901 tgcaggtgga cgacgagccg aatgtcaggg gcgcccacag gcgcgtagag cctggcatgg
      961 tcgtgagtcc ccagggggtc tggccctcgc tggagtacga cgtgaagtcg gccaataggg
     1021 ctttcggccg ctttctctcc gccggtttct actacaagac cttcatcaag ccccgccggc
     1081 tgtggccctt ataccggcgg atgctgcggc gtttctccgc gggcggcaga gtctcagccg
     1141 gtcctgcgca aggttacttc gacaagcgct acgcccaccc cgacgtcctc gtcgccggcg
     1201 gcggtcccgc ggggatggcg gcggcggtcg cggccggaag agcgggggcg caagtcttgc
     1261 tggtcgagga agagtacgag ctcggcggcc atctacgttg ggggaacgag ctggatgcct
     1321 tgcgcgtact gcgtgaggag gtgtcccgca cgccgggcgt cgaagtgatg gtcaactccg
     1381 tcgtcaacgg gcgctacgac gacaactggg tcgcggtgat gcaacgcgac ctgtcccatg
     1441 tccaggaacg gctcgtcaag gccagggcca agacgctagt ggtggcacct ggcctcatag
     1501 aacgccccta cgtcttcgag ggcaacgata cgccaggggt catcctctcc accgccgcgc
     1561 ggcgtttgat caacctctac gccgtcaagc caggagaccg tgcggtcgtc ttctccgcga
     1621 acgagagcgg cgacgcggcg gcggaagacc tcagacgctc gggcgtcgag gtcgcacgcg
     1681 tcgtcgacgc ccgcagggac ggggacatcc ttcgcgcgaa gggcgacggc aagggcttac
     1741 gctccgtgga gtgcgccgac ggcgcagaga tagagtgcga cctcctcgtc acggccgcag
     1801 gctggaccgc gccgacttca ctcgcgaaca tggcaggaga caggccggtc tacgacgagc
     1861 gagccgcccg cttcttcccc tcgatcctcc cggaagacct gcttgccacc ggcggtatcg
     1921 cgggcgacgg gacgctcgac gaactgctag cccacgcgcg cgccacaggt caggaagctg
     1981 cccggcgggc cgtgaggacc gcactgcggc tgcgcgccgg cataccgcag cgcgcggacg
     2041 agcaggaaga gcccgagtca gggtcatcgc cggtggcaat ccctgagttg ccgatcagcg
     2101 cccatcccgc gctgttccgc ggcaagacgc acggcatcgt agactactcc gaggacgtca
     2161 cctccaaaga cctcgtcgcg gccgagaagg agggctacga ctctattgag ctggtcaagc
     2221 ggtacacgac cgtgaccatg gggcccaccc agggcaagct cgagaccgtc aacgcggttg
     2281 cgattatggc ggaggctacg gggcgcacca tcaatgagac agggaccacc gtatggcggc
     2341 cgccgtacag cccgatcacc cttggcgcct tggcagggcg caacttcgag ccggtgcgct
     2401 actctccgat gcagccgtgg cacgagatcc acggtgcaaa gccgctggtg gcgggggcct
     2461 ggatccgccc cgaccattac ggcgaccccg cctccgaggc cctgaacgtc cgcgagaacg
     2521 tcggcatcat agacgttacg cctctgggca agttcgacct gcgcggcccg gacgtgccca
     2581 aactgctcga ccacctctat atcaacaggt gggacaacct cgaagtcggc gcggtccgct
     2641 acggcgtgat gtgcctcgag gacggcgtgg tcttcgacga cggcgtgacc ggctgcctcg
     2701 gcccagagcg ttacatcatg acgacgacct cctcgggcgc cgccggcgtg tgggagtgga
     2761 tcgaccattg gctgcagacc gcagacccgg ggtggcgaat ccacgtcacc cccgtaacca
     2821 cggcgtacgc aagcatcaac gtcgccggac caaaatcccg cgaattactc ctgcgcctga
     2881 gcgatggcct tgacctctcg cctgaggcct tcccctacat gcgggtgcgc gaggggcgcg
     2941 tcgccggcgt cgaaggctgc tttatgtggc gcatcggctt cacaggcgag ctcagctacg
     3001 aggtccacgt gcctgccgcc tacgggctac acgtgtggga gacgctcatg cagagcggcg
     3061 ctgacctcgg cgttgggccc ttcggcgtag aggcgcagcg cattctgcgc ctggagaagg
     3121 gacacttcat cgtgggccag gacaccgacg gactcacagg accatacgaa gtgggcctcg
     3181 gccggatggt caggctggac aaggacgact tcgcgggcaa gcccgagctg gcgtggcaga
     3241 gcgagcgcga cagccgcctt cggctcgtcg gcctgcagcc gcacgacggc tcgctggtcc
     3301 cccctgaagc gagccagatc gtggagaata agaacgagat cgtcggccgg ataacgtcga
     3361 gccgcatgtc gcccactctg gggcgttcga tctgcctggg cttcgttgcc cagcatctga
     3421 ccacgcctgg gacgaaggtc acagtgcgac tgcccgacgg agagcgcata ccggcctccg
     3481 tcacaccgca ccacgcgcac tacgaccccg aagggacgcg ccagcgtggt tgaggcagcg
     3541 cccatcgccc gcagccctat agcgcaggcc gctcccacga ccatcaagca cggctgggag
     3601 gtcagcgccc gccgcagcgc agcagacctg aagatcattg actgtacccc cctcgccaag
     3661 atcctcgtgt tggcgtcccc ggacggcgaa gtcgcccgcg ctctgggtgt ccccttcgga
     3721 cgcgccgccc gcgacccgca cgggacgctc gtcgtgggct caggccccgg tgaatggctc
     3781 ttgcttgcta caccaggaac gagtccggcg gtcacacagc gtgttgatga ggtgcaggat
     3841 gagggtctcg tcagcgtctt cgacgccacc cacgggcggg cgatcatgcg gatcacggga
     3901 gccaaggcgc ccgatctgct ggcgaaagtg tgcgccatcg atctcgccga cgaggttacg
     3961 cccaacggtg cggccttccg tagttccgtg gcgaagctcg taaccgatgt cgtgcgcgac
     4021 gacagcgggg gatcgcggtc gtacctgctg cattgtgagc ggtcctcggg tcagtacctc
     4081 ttcgacgcgt tgatcgacgc gggcgacgag ttcggcatag aggtcgacgg gttcgtcgac
     4141 ccaggaaaaa gaggagtcca cagagtatga cgttaccgaa gaacctggat gtcgccaggc
     4201 aggctaagct cctgccgctg aacgaggtcg ccgctgagat aggcatcggt gagcacctcc
     4261 tggagcccta cggagagggc gtcgccaaga taaagctcga cgccatcgag gaactcgccg
     4321 acaggccgaa ggccaaatac gtggtgatct ctgccataac gccaacgccc ctgggcgagg
     4381 ggaagaccac gaccaccgtt ggcctggggc aggccttcaa gcacatcggc aagacggcga
     4441 cggtggcagt gcgccaggcc tcgatgggcc ccgttttcgg catcaagggc ggggccgccg
     4501 gcggcggtta cagtcaggta gtgccgatgg agacgctcaa tttgcacctc accggcgaca
     4561 tccacgcggt caccgaggcc cacaaccagc tcgccgccat gatcgacaac cacctctacc
     4621 agggcaacgc cctcgacctc gagccacaca gcatcacctg gcggcgggtg atggacgtaa
     4681 acgacagggc cctgagacac atcgtcgcgg ggcttggcgc caggaccgat ggcgtcccgc
     4741 gccagaccgg cttcgatatc acggcggcct ccgaggtgat ggcggttctc tcgctctcca
     4801 cctcgcttca ggacatgcgc agccgcttcg ggcgaatcgt ggtcggcacc gacgccgagg
     4861 gcagtcccgt gactgccgaa gacctaaagg ccgcgggtgt catgacggtc atcatgaaag
     4921 aagccataaa gcccaacctc atgcagaccc tggagaacac gcctgccctg gtccacgcag
     4981 ggcccttcgg caacatcgcc actggcaact cctccatcgt ggccgacgaa gtcggtatcc
     5041 gcgggggcga ctacctcgtg accgaggcag gcttcggcgc cgacatgggc gccgagcgct
     5101 tcttcaacat caagtgccgc tactcgggcc tcgaaccgga cgccgcggtg atcgtcgcca
     5161 cagtgcgggc gctcaaggcc cactctggtc gccacgaggt caaagcaggc aagcccctcc
     5221 ccgaggagat gcaaaaggag aaccccgatg acgtttatgg cggcgccgcc aacctaaaga
     5281 agcagataga gaacatccgc ctccacggcg tctctccggt ggtagcgatc aacgccttcc
     5341 ccacagacca cgagacggaa tacgacgcca tccgcgagat cgcaggggag atgggcgcgc
     5401 gggtggcggt atgcaaccac ttctcggagg gtggggctgg cgccgtggag ctcgccgagg
     5461 cggtcgccga agccgccgaa gagcctacct cgttccggtt cctctaccct gatgaggttt
     5521 ctttgagaga gaagatcgag accatcgcca caaaggtgta cggggcgaaa gacgtcgagt
     5581 acaacccgca ggcggcgagg atgctcgact cctacgagac cagcggcttc ggcgagttgc
     5641 ccgtctgcat ggccaagacg catctctcca ttacttcaga tccatcgctc aaaggagcgc
     5701 ccacgggctg gacgctcccg gtcagggagg tcagggcgtc ggtgggggcc gggttcgtct
     5761 accctatctg cggcgacatg cgcacgatgc cggggctcag cgcttctccc gccgccgagg
     5821 gcatagacat agatgaggac ggcgagatag tggggttgtt ctgagcgagc cgcataggac
     5881 agcgatcgat gtgttctcta cagacgaatt gcgggcgccc ggaggtgatg agaaaatgca
     5941 gctacccacg cgagcctctt acgtcgtcat cggggccggg gttcacggcc tgagcacggc
     6001 ctggcacctg gcgaaagaat tgaaggccag ggggcgcggc tcaggcgagg acgtgatcgt
     6061 gctcgacaag accggcgtcg ccgcaggggc ctcggggatc gcttgcggtg tgatccgtaa
     6121 caattacttc cagccagcga tgcgcaagct gatggcccat tctgtctcca tctgggagtc
     6181 ggacccggaa gcgttcgcct accatcccgt cggctacatg cagatctcac cagaggtgat
     6241 gcaccacgac gtggtgcaga tctataagga gcagcaggcg atcggctacc cctctactct
     6301 cgtcgaaggg gaagaggagt gccgctccta catgcagaac atcttccacg actggcaggc
     6361 cgagaacatc acgagcatcc tgcacgagca ccgcggcggg tacgcgaaca acaaggcctc
     6421 ggtaaggacc cttgccgcca aagcccaggc cgagggggtg cggatccaca ccggggtcag
     6481 ggttaccgga tttagtatgg aagacgaacg tgtgagatcc gtacagacgg attggggcgc
     6541 ggtcggctgc gagcacctcg tcgtggccgt cggcccgtgg gtcagggaca tctgggcgat
     6601 gctcgacctg cccgagaaga tcaccgtcaa gggtcctgat ggcgagtcgt accgcgaccg
     6661 cgagatgtgg acgtactggt cgctgcggga gggcgtgctc ggtgtcgaac cgagcttcct
     6721 tacggacaac gaaggtcgca tgccgccggt aatccacgta gacaccgatg ccccgctata
     6781 cgacgaagac ggcgagctcg tcacggacga actctggggg atctactaca agccggactt
     6841 ctacttcaac ggggtacagg gcggggcaac tccctacgtc gtcgaagggc cagcgagtga
     6901 ggtcgcagtc gacccctacg ggcccgagag ccccgacttc gtggtaggag aagacttcgc
     6961 caggatgtgg acctcggcgc tggcgcactg ccacgggcgc ttcgagggga agggctacct
     7021 gttctccagg gagccctcgg gcggtatcgg gtgcttcacc cccgatagct tccccgtctt
     7081 cgacaccttc agggacaatg cctacgtcat cgccgactcc aaccacggct acaagatgat
     7141 cggggtcggt gccctggtgg cgaaggagct tatggacgag ccgcaggaac agctggaacc
     7201 tttccgtttc tcccgctacg agaccggaga actgcaccca acgagcaact ctcctttccc
     7261 atggagctga cacagggaga ctccgaggat ttgcgtagtt catcttcttc caaataccgg
     7321 gtggacaggc tcaggatcga atgcgccgtc agagagttgc ttatagcaat aggcgaagac
     7381 ccggaacggg aaggactcag aggcacgccg caccgcgtcg ccgaagccta cgcgtccctc
     7441 ttcgccgggc tggctgatga cccggccagg catcttgaca tggggtttca agaagaacat
     7501 cgcgagatgg ttctcgtacg ggacatacct ctgcattcac tctgcgaaca tcacctcatg
     7561 cccttcgtcg gcaaggccca cgtgggctac ataccagacg ggaaggtcgt aggcttgtcc
     7621 aagctggccc ggttggtgga gggctacgcc cgtcgccccc agctccagga gcgtcttacc
     7681 gcccaggtcg ccgacgccat ccacgagagg ttgggcgctc gtggcgccat cttcgtcgtc
     7741 gaggcggaac atctgtgcat gacgatgagg ggagtgcaga aaccagggag cgttacggtc
     7801 acaagcgccg tgcgcggcat ctacgccaga gac
//
LOCUS       DASTKM010000179         5021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1458071, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000179 DASTKM010000000
VERSION     DASTKM010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458071"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /gene="menC"
                     /locus_tag="VFQ10_07430"
     CDS             complement(<1..671)
                     /gene="menC"
                     /locus_tag="VFQ10_07430"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET9965210.1"
                     /translation="MKLARFDLYRYSLPFSRPLTLGGITLFQREGILLRLSGEDGSEG
                     WGETAPLPGFSVEGLVDATAQLRRLAASIVGREVKQDWVDPYGEFGRGLDRVAPSVRF
                     GLELAVWNLYGDSSGRSLAALMAPSPRSVVPVNGLLAGSPAEVLEEARSMRDAGYSCI
                     KLKVGTRTVAEDAALVHALGEDLGEGISLRLDANRAWGYEEAAEFVGGTAGVRFEYVE
                     EPLADP"
     gene            867..2066
                     /locus_tag="VFQ10_07435"
     CDS             867..2066
                     /locus_tag="VFQ10_07435"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET9965211.1"
                     /translation="MGHVRRRLTDGASQRASTLELFYDLVFVFAITQVSHLLLEHLSW
                     AGVLQSLIALLAVYWSWNYTTWTTNELDTETVPVRLLLLALMLVSLLMAVAIPQAFEA
                     HALLFAGSYVAIQIGRHSFLTFAAADRGTVERERAAHILIWFMVVGALWVAGAMLDGQ
                     ERILLWLVALALDYSAPLVTYWIPGRRRLAPATWNVGTEHFAERFGLFIILALGESIV
                     ITGATTSDLELDAARIIAFIMAFLASAALWWLYFSSVANLGAHYLEVSENRTILARDA
                     YTYLHVVFVAGIILSAVGDELVIAHPTETLPPYEVATVAAGPAVYLFAHTLFGYRLTG
                     LVYKTKLFGTLACVAVGFVGLLVPALVLAGLLIVVLVAVIGSSHLAAPRAEAVGEAPP
                     RSGLRKG"
     gene            complement(2134..2985)
                     /gene="menB"
                     /locus_tag="VFQ10_07440"
     CDS             complement(2134..2985)
                     /gene="menB"
                     /locus_tag="VFQ10_07440"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HET9965212.1"
                     /translation="MTNLQWESAGKFEDIRYEKADGIAKITINRPEVRNAFRPLTVIE
                     MQRALEDAREDHETGVIILTGEGPDAFCSGGDQKVRGDAGYLEDPDSPRRTPGGESVG
                     RFHVTDLHVQIRRCPKPVVAMVAGYAVGGGHVLHVVCDLTIAADNAKFGQVGPKVGSF
                     DGGFGASVLASLIGPKKAKEMWFLCRMYSAEEALQMGLVNEVVPLATLEEETVCWCRE
                     MLDRSPFALRLLKASFHAHEDGYAGIQQLAHDANLLFYGSEEAQEGRDAYKEKRRPDF
                     SRFPHRP"
     gene            3079..3567
                     /locus_tag="VFQ10_07445"
     CDS             3079..3567
                     /locus_tag="VFQ10_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9965213.1"
                     /translation="MYSLYMHPQTHRSLTQAREFMRHAYSHPVSLPDVAARANLSPYH
                     FLRVYKRAYGETPHEFLTRLRMERAKTLLARGSHNVTEACFEVGFSSLGSFSNLFAYR
                     VGLSPSEYRRYARSTISVPSTVRTLFVPSCFFTMLYGTQEERNIREAGRLETMVSSGQ
                     ST"
     gene            3583..3999
                     /locus_tag="VFQ10_07450"
     CDS             3583..3999
                     /locus_tag="VFQ10_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9965214.1"
                     /translation="MIQRQSHSTIYVIDQEEALAFYRDILGFEVNTDMPMDDSGLRWL
                     TLNPKGQPDLELILAQVTAGPMFDAETAEEMRAMVERGAFGIGVFETDDIQSDYERLS
                     SQGVEFVSPPQERFYGTEAIVKDNSGNWFSLTQSKR"
     gene            complement(3961..4788)
                     /gene="menH"
                     /locus_tag="VFQ10_07455"
     CDS             complement(3961..4788)
                     /gene="menH"
                     /locus_tag="VFQ10_07455"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HET9965215.1"
                     /translation="MPDNLNHEVSGDRLSPTILFLHGFMGSSADWRGAIAALGYRYFC
                     IAVDLPGHGASLGMPPDTYTIEGATRTVIGILDELERVRTVIAGYSMGGRLALYLALR
                     YPERCAGLFLESASPGLESAGERAARRAADEAKAGRLESGDFEAFLQDWYRQPLFASL
                     ARDENLLRRTVEARRRNDPAELARSLRGMGTGSQPSLWGELEGLAVPALAVAGGLDEK
                     YAGISSRMASINPRIESAIVPGAGHSVHAETPAGYTSLLGRFVGGLSFALGETEPVP"
     gene            complement(4819..>5021)
                     /locus_tag="VFQ10_07460"
     CDS             complement(4819..>5021)
                     /locus_tag="VFQ10_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylate synthase"
                     /protein_id="HET9965216.1"
                     /translation="VGFEPAAKMFGLAYEHPGTIEDFVEAYGSACSRGSSTLIEIDTD
                     RKENVSVHRRLLQEVADRVAKA"
BASE COUNT          901 a         1631 c         1506 g          983 t
ORIGIN      
        1 ggatcggcga gcggctcctc gacgtactcg aagcgtaccc ccgccgtgcc acctacgaac
       61 tcagccgcct cctcgtagcc ccacgcccgg ttcgcgtcga gacgcaggga gataccttcg
      121 cctaggtcct cacccagcgc atggacgagg gcggcgtcct cggcgacggt ccttgtgccg
      181 accttgagct tgatgcaact gtaacccgcg tccctcatgc tccgggcctc ttcgagtact
      241 tcggcggggg atcctgcgag caagccgttt accggtacca cggacctcgg agagggcgcc
      301 atcagagccg ccagcgacct tccggaagaa tccccgtaca ggttccagac cgccagctcg
      361 agcccgaacc ggacagaggg tgctacacgg tccagcccac gaccaaactc tccgtacggg
      421 tccacccagt cctgcttgac ctcacggcct actatcgacg cggcgagccg ccgcagctgc
      481 gccgtggcgt cgaccagacc ctccacgctg aagcccggaa gcggagcggt ctcgccccag
      541 ccttctgaac catcttcgcc ggacagtctc aggaggatgc cttcgcgctg aaagagcgta
      601 atgcccccta gcgtcaacgg cctggagaaa ggcaggctgt agcggtagag gtcgaagcgg
      661 gctagcttca tcccggtaac tcctgacatc ataaaggtgt catctggtcg tcatattgac
      721 acctgtcgtg aacgggtgca caaaagaggt gtgcttccgt attcgcaaga gtggaatgac
      781 acgaaggaca gcctcgcccg tcgcgctttc gggacctgac tatggcggcc ggggcagatc
      841 gtccctaacg tggttagact gctctcgtgg gacatgtgag gcggcggctg actgatgggg
      901 cgagccagag ggcttcgacc ctcgagctct tctacgacct cgtgttcgta ttcgcgatca
      961 cgcaggtctc ccacctcctg ctcgagcacc tgtcgtgggc cggcgtcctg cagtccctga
     1021 tagctcttct cgcggtgtac tggtcgtgga actacacgac ctggaccact aacgaactgg
     1081 ataccgagac cgtacctgtc cgtctgctgc tcctggccct gatgctcgtc agccttctca
     1141 tggcggtggc gataccgcag gctttcgagg cgcacgcgct actgtttgcc gggtcgtatg
     1201 tcgccataca gataggcagg cactccttcc tgactttcgc ggcggcggat cgcgggacgg
     1261 tcgaacgcga gcgtgctgca cacatcctca tttggtttat ggttgttggc gcgctttggg
     1321 tggcaggcgc catgctagac ggtcaggaac gcatactgct ctggctggtc gcgctcgcgc
     1381 tcgactacag cgcgccgctc gtgacttact ggataccggg ccggcggcgc ctcgcgcccg
     1441 ccacctggaa cgtgggaacg gagcacttcg ccgagcgctt cggcctgttc atcatcctcg
     1501 ctctcggcga gtccatcgtc ataaccggcg cgaccacctc cgacctcgaa ctcgacgcgg
     1561 cgaggatcat tgccttcatc atggccttcc tggcgtccgc ggccctctgg tggctgtact
     1621 tctcgtccgt ggcgaatcta ggagcacact acctcgaggt ctccgagaac agaactatcc
     1681 tcgccagaga cgcctacacc tatctgcacg tggtgttcgt cgccgggatc atcctctccg
     1741 cggtgggaga cgagctggtg atcgcccacc cgacggagac actgcctccc tacgaggtcg
     1801 ccacggtggc ggctgggccg gccgtgtacc tcttcgccca taccctcttc ggctacaggc
     1861 taacggggtt ggtgtacaaa acgaagctct tcgggacgct ggcctgcgtc gctgtcggct
     1921 ttgtcggcct cctcgtgccc gcgctggtgt tagcagggct gctaatcgtg gtacttgtgg
     1981 cggtgatcgg atctagccat ctggcagctc cgagggccga agcagtaggc gaagctccgc
     2041 ctcgaagcgg cttgcggaag ggctgagctt tgcacaggcc aggtatcagg ctgtgcacgg
     2101 ggtagcctct tgatgaatac gccgggggtg tggtcaggga cggtggggaa acctcgagaa
     2161 gtcgggtctg cgtttctcct tgtaggcgtc gcggccttcc tgtgcctcct cgctgccgta
     2221 gaagagtagg ttagcgtcgt gggcgagctg ctggatgccg gcatagccgt cctcgtgggc
     2281 gtggaagctc gccttcagga ggcgcagcgc gaaaggtgag cggtccagca tctccctaca
     2341 ccagcagacg gtctcctcct cgagtgtggc gagcggcacg acctcgttga ctagtcccat
     2401 ctgtagcgcc tcctcggccg agtacatcct gcagaggaac cacatttcct tcgccttctt
     2461 cgggccgatg aggctggcga gtacagaagc accaaaaccg ccgtcgaagc tgccgacttt
     2521 cggaccgact tgcccgaact tcgcgttgtc ggcggcgatg gtgaggtcgc acaccacgtg
     2581 caggacgtgg cctccgccga cggcgtaccc tgccaccatc gcgacgacgg gcttggggca
     2641 gcgccggatc tggacgtgca gatccgtgac gtggaagcgc cctacggact ctcctcccgg
     2701 ggtgcgacgg ggcgagtccg ggtcttccag gtatccggcg tctcctcgta ccttctggtc
     2761 ccccccggaa cagaaagcgt caggcccctc tcccgtcagg atgatgaccc cggtctcgtg
     2821 gtcctcgcgg gcgtcttcga gagccctctg catctcgatg accgtcagcg gacgaaaagc
     2881 gttcctcacc tcggggcggt ttatggtgat cttcgcgata ccatccgcct tctcgtacct
     2941 gatgtcttcg aatttcccgg ccgattccca ctgtaggttt gtcatgtagc cgattatatg
     3001 ggatctagac acccctcatc gaggttcttc atcgcaagag ctcctctcca ataacgccgt
     3061 tgcttcccag actacgtcat gtactctctc tacatgcacc ctcagacgca caggagcctc
     3121 acccaggcca gggagttcat gcgccacgcc tacagtcatc ccgtctctct cccggacgtc
     3181 gcggccaggg ctaacctatc cccctatcac ttcctgcgcg tctacaagcg ggcctacggg
     3241 gagacaccgc acgagttcct gacgcggctg cgcatggaga gggcgaagac gctgctggcg
     3301 agagggagtc acaacgttac cgaagcctgc ttcgaggttg gcttctccag cctgggcagc
     3361 ttcagtaacc tgttcgcgta tcgcgtgggt ctatctccct ccgagtacag gcgctacgct
     3421 cgctcgacga tctctgttcc gtctacggtc aggacgctgt tcgttccttc gtgctttttc
     3481 accatgttgt acgggactca agaagagcgc aatattcgag aagctgggag gctagagacg
     3541 atggtatctt cgggccagag cacctagaca gggaggtaag ccgtgattca acgccagagc
     3601 cactctacga tctacgtgat cgaccaggaa gaagccctcg cgttctaccg cgacattctg
     3661 ggcttcgagg tcaataccga catgcccatg gacgactctg gtcttcgctg gctgacgttg
     3721 aacccgaaag gtcagcccga cctcgagctg atcctggccc aggtaaccgc cggccctatg
     3781 tttgacgcgg agacggcgga agagatgagg gcgatggtcg agagaggggc cttcggcatc
     3841 ggtgtattcg agacggatga catccaaagc gactacgagc gcttgagcag ccagggtgtc
     3901 gagttcgtgt ccccacccca ggaacggttc tacggcacag aggccatcgt gaaagataac
     3961 tcagggaact ggttcagtct cacccagagc aaacgatagt ccacccacaa aacgcccgag
     4021 caaggaggtg tatcccgccg gggtctcggc gtgcacgcta tgtcccgctc cggggacgat
     4081 tgcggattct attcgcgggt tgatgctcgc catgcgcgat gagatcccgg cgtatttctc
     4141 atccagccct ccggcgacgg cgagcgccgg cacggcgaga ccttccagct ctccccacaa
     4201 cgacggttgg ctcccggtcc ccatcccccg caatgagcgc gccagctctg caggatcgtt
     4261 gcgccgccgc gcctcgacgg tccgacggag caggttctcg tcccgtgcca gagacgcgaa
     4321 gagcggctgg cggtaccagt cctgaagaaa cgcctcgaag tctcccgatt ccagacgacc
     4381 tgccttcgcc tcatccgctg cgcggcgcgc tgctcgctca ccggcgctct ccaggccggg
     4441 cgaggctgat tcgaggaaga gccccgcgca ccgttccggg taacgcaggg ccagatacaa
     4501 tgccagccgt ccgcccatag agtaaccggc gattacggta cggacgcgct cgagttcgtc
     4561 caggataccg atcaccgtcc gtgttgcacc ctcgatcgta tacgtatcag gcggcattcc
     4621 caacgatgcg ccgtgccccg gcagatccac ggcgatgcag aaatatcggt atccaagcgc
     4681 cgctatggcc cctcgccagt cagcggacga acccataaag ccgtgcagga agaggatcgt
     4741 gggagacagg cggtcgcccg agacctcatg gttcagatta tccggcatgc gcacatcgta
     4801 tgatcagagc agcgcccatc acgccttcgc tacccggtcc gccacctctt ggagtaatct
     4861 gcggtgcacc gacacgttct ccttgcggtc ggtatctatt tcgatcagag tcgaggagcc
     4921 gcgggaacac gcggagccgt acgcttcgac gaagtcctcg atcgtgccgg gatgctcgta
     4981 agcgaggccg aacatcttcg ccgctggctc gaagcctacg t
//
LOCUS       DASTKM010000180         3500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_754040, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000180 DASTKM010000000
VERSION     DASTKM010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754040"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFQ10_07465"
     CDS             <1..222
                     /locus_tag="VFQ10_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965217.1"
                     /translation="DHINSWGGDDVVLGYRGADKIRGGDDNDKQYGGRGNDVIYSEGG
                     FRDLVYGGRGTDTCYVDSKDRLKGCERKR"
     gene            764..2677
                     /gene="thrS"
                     /locus_tag="VFQ10_07470"
     CDS             764..2677
                     /gene="thrS"
                     /locus_tag="VFQ10_07470"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET9965218.1"
                     /translation="MASVKLPDGKQLEIEPGEKARDVAEKIGKRLARDAVVAKLDGEL
                     IDLDAPLNGGGDFEVVTIDSPEGLEVLRHSTAHAMAQAIVELYPGSKLTIGPPVENGF
                     YYDIEVEGRLTDEDLPRIEERMHEIVARDLPIQREDVSKAEAEDLYQDNPYKTELIEG
                     LEDGEISIYKQGDFFDLCRGPHVPSTGRLGAFRLQNIAGAYWRGDEKNPMLTRVYGTA
                     WPTEKELKAYLRRLDEARARDHRKIGKDLDLFTFSPDTGAGIPLFLPKGEMMRHLMED
                     YVREVQTSHGYEHVWTGHLVNEALFAKSGHLEHYRESMFPPMIDGETRYRLKPMNCPS
                     HMTLYNSRSHSYRDLPLRYAEFATLYRYEKSGELTGLTRVRSLTQDDAHVFCTEDQIQ
                     DEFARALEIVREVLDTYGFADYRVQLSLRDQDVAKYVADDAKWSRAEKELREALDTAA
                     IAYHEEAGEAAFYGPKADFLAKDVLGREWQLSTIQVDFIQPARLGCEYIGEDGKAHTP
                     VLLHRAVTGSTERFMAVLIEHFAGAFPVWLSPVQAVVIPIADRHLDYARGVQVQLAER
                     DLRVEVDDSQNSMQKKIRDNSRQKIPYLLIVGDREAEEGSVNVRRRGEKQQQEMALVD
                     FVEAVSREVRSRG"
     gene            2840..3394
                     /gene="infC"
                     /locus_tag="VFQ10_07475"
                     /pseudo
     CDS             2840..3394
                     /gene="infC"
                     /locus_tag="VFQ10_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
BASE COUNT          789 a         1000 c         1122 g          589 t
ORIGIN      
        1 gaccacatca actcatgggg gggcgacgac gtggttttgg gctatcgggg cgccgacaag
       61 atacgcggtg gtgacgacaa cgataagcaa tacggcggtc ggggcaacga cgttatctac
      121 agtgagggcg gcttcaggga cctggtctat ggcggcagag gtaccgacac gtgctatgtg
      181 gactccaagg accggctgaa gggctgtgag aggaaaaggt gaggtaggag ggcaccgaag
      241 gtcctatgag acctctttag gtcttatcag gtccgcctcc aagccgacgc tctcccgggg
      301 ccggggtgag caaccccggc cctttctctg ggcctcgttc gcccaacctc gtagaaggta
      361 gattctccga aatccgcatg cgcccttgag gacagtgtgt agctcagggg acggccacgc
      421 accgagaatg tttagtcgcc gtgtagcttc gtcaccacat gatgtgaagg tggatagaca
      481 tataccgtcg cgcgtgtcag aactgtgagt tatcctcggg cagaaccgga gcaccaaggt
      541 ccataacgag agaacccaag tccggaatat cccgcaatta gccaggattg tgtaaactta
      601 ggaaaggtat ggaaaagcat tacacatctt tgacgcggaa gaggtcagtg gttcgacctc
      661 actatcgccc accacgctgc gcggattcca ccggcgcgct gcgtcgaact acctagttgc
      721 tacccgattt ctctcaccca ccattggcaa gaaaggtgca caaatggcct ccgtcaagct
      781 acccgacggt aaacaactgg agatagagcc gggcgagaag gcccgggacg tcgccgagaa
      841 gataggtaaa cgcctcgccc gggacgcggt ggtcgcgaag ctggatggag agctcatcga
      901 cctcgatgcc ccgctgaacg gtgggggaga tttcgaggtc gttaccattg actcacccga
      961 gggcctcgag gtcttgcggc attcgactgc gcatgcgatg gcgcaggcta tcgtagagct
     1021 ctatccaggg agcaagctga ctatcggccc gcctgtagag aacggcttct attacgacat
     1081 cgaggtagag ggacgcctca cggacgagga tctgccgcgc atcgaggagc ggatgcatga
     1141 gatcgtcgcg cgggaccttc cgatacagcg cgaggatgtc tccaaggccg aggccgagga
     1201 tctctaccag gacaacccgt acaagacgga gctcatcgag ggtctggaag acggtgagat
     1261 ctcgatctac aaacagggag attttttcga tttgtgccgc ggcccgcacg tccctagcac
     1321 gggcaggctc ggcgccttca ggctgcagaa catagctgga gcctactggc ggggcgacga
     1381 gaagaacccg atgctcaccc gcgtctacgg tacggcatgg ccaacggaga aggaactcaa
     1441 agcttacctc aggcgcctcg atgaggcgag ggcgagggac caccgcaaga tcggcaagga
     1501 cctcgatctg ttcacgttct cgcccgatac aggtgccggt atcccgctct tcttgcccaa
     1561 aggggagatg atgcgtcacc tcatggaaga ttacgtgcgc gaggtccaga cgagccacgg
     1621 ctacgagcac gtgtggaccg ggcacctggt caacgaggcc ctgtttgcga agtcgggaca
     1681 tctggagcac tacagggagt cgatgttccc accaatgatc gacggcgaaa cgcgttacag
     1741 actcaagccc atgaactgcc caagccacat gacgctctac aactccaggt ctcactcgta
     1801 ccgcgatctc ccactcaggt acgcggagtt cgcgacgctg taccgctacg agaagagcgg
     1861 ggagctcaca gggctcaccc gggtgcgtag cctgacccag gacgacgcgc acgtgttctg
     1921 tacggaggat cagatccagg acgaattcgc ccgggcactg gagatcgtgc gggaagtcct
     1981 cgatacctac gggttcgccg actaccgggt tcagctctct ttgcgcgacc aggacgtggc
     2041 caagtacgtg gctgacgacg ccaagtggtc gcgggccgag aaggagctgc gcgaggctct
     2101 cgatacggcc gccatcgcct accacgaaga ggccggggag gcggccttct acgggccgaa
     2161 ggccgacttt ttggccaagg acgtgctggg acgtgagtgg cagctctcca cgatccaggt
     2221 ggacttcatc cagcctgcca ggctaggttg tgagtacatc ggcgaggacg gcaaggcgca
     2281 tacgcccgtg ctcttgcaca gggccgtcac cggttccaca gagaggttca tggcggtgct
     2341 gatcgagcac ttcgctggcg cgttccccgt ctggctctcc cctgttcagg ctgtagttat
     2401 ccccatagcc gacaggcacc tggactacgc ccgcggggtt caggtgcagc tcgccgagag
     2461 agatctgcgg gtcgaggtgg acgactcgca gaacagcatg cagaagaaga tcagagacaa
     2521 ctcgcggcag aagatcccct acctcctgat agtcggagac agggaggccg aggagggtag
     2581 cgtcaacgtg cgacggcgcg gggagaagca acagcaagag atggcgctgg ttgacttcgt
     2641 agaggccgtc tcccgcgagg tccgctctcg cgggtagtgg tgcatagaca cgaggctcgg
     2701 gaccgcggta taatcacgcc tgtagttggg gtccgcgtcc gtccgacgca cgagcagatt
     2761 tatcgtccga ggtggacgaa cggccccgta atctcgcttt cgagaggagt ggtgcgcagt
     2821 agcgactgaa gaagagcccc gaataaacgg tcagatccgg gcgcgcgagg tgcgtttgat
     2881 ctcggccgac ggcgaacagc ttggcatcaa gcacatccgc gaggccatgg attatagtga
     2941 gagagccgac ctcgacctcg tggaggtagc cccgaatgcc aacccccccg tggtccgaat
     3001 gatggactat ggcaagttca agtaccagaa agagcaggcc cgcaaggcgg cccgcaagaa
     3061 gcaggtcaac gtcaacgtgc gggagataaa gctcaggccc aagataggcg accacgactt
     3121 caacaccaaa cgcggccacg tcgaacgctt tcttcgaggc ggcgacaaag tgaaggtcac
     3181 gatcatgttc cgcgggcgcg aggtgcagca tcccgagctc ggagagagac ttctgaggag
     3241 gctcgcgagc gacctcgacg gactcggtcg catagagagc cagcccaacc tggacgggcg
     3301 caacatggta atggtcatgg ccccgaaaaa ggagtcctcc caggcggaga ccccccagtc
     3361 gagcgaacgc agggagaggg cggctcgcag ataagcaaca gtttgataca ctagcctggc
     3421 gaccgacaga ggcgaaacgg ctctttctcg cgtgccgcga gcctcttcgc aggtagacag
     3481 agcagacgaa acgcggagga
//
LOCUS       DASTKM010000181         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1106736, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000181 DASTKM010000000
VERSION     DASTKM010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106736"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VFQ10_07480"
     CDS             complement(<1..1394)
                     /locus_tag="VFQ10_07480"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET9965219.1"
                     /translation="MANGGISRAAPSGNLRRFSQISRVLVRHGFGFVFDVRRDRREKK
                     GLQEFLAPNFGVRLRRTLDDLGPTFVKFGQVLSTRSDILPEGVLSELEKLQDTATPMP
                     AGVAQEIIEGELGAPVDELFASFDPVPLGSASIGQVHRAVLRGGESVAIKVQRPEAPG
                     RVESDLELMRDFAALLDRRFGRRLFVDVRSLVAEFEVVIRRELDYSQEAENARRFAAN
                     FAGTQVIIPGVYLEISTSRVLTEEYIEGTRFRDIRPLVLTPSERRRVASMGAEAIFKM
                     AFEDGFFHGDPHPSNLLLTPTGDLALLDFGMVGYMSQGDIEALSRLFIAVVQRDAASA
                     LLALERLGVRYATEVRSDLIRDLREFLNKYSGLSVGEVTLGQALSELVALARRYRLRM
                     PPVFPLLTKALVTAEGLARSIDPTINVYEVARPYAQRLLSGRYSPEAVIEVAEERALE
                     YARYFEDYPEQIRLL"
     gene            complement(1410..1811)
                     /locus_tag="VFQ10_07485"
     CDS             complement(1410..1811)
                     /locus_tag="VFQ10_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965220.1"
                     /translation="MRGTVGETLERLILLQIGAAAATRDKVQEIVDSLIEQGRVEREQ
                     GRTVVNDVMNRARERSTGARSLVDASLQQGLRRGGLPTREDYEDLLFRIEQLEHRVRM
                     LEDRPAAPPPPAGEPESPPERGDAPETPPGL"
     gene            complement(1880..2770)
                     /gene="secF"
                     /locus_tag="VFQ10_07490"
     CDS             complement(1880..2770)
                     /gene="secF"
                     /locus_tag="VFQ10_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET9965221.1"
                     /translation="MFEKVDFVGGWKLYFSISAVLLIAGAVAIALGGLNFGIDFQGGS
                     QFTVANAERQLSDEQVRAALPEDLAGESVVTTLGQDGYEVRTPVISSQEEQSQVRESL
                     SRAFGGEVSVTTVSPAFGQQLRNQALQAVVAALIIIVVFISFRFEFAFALAAMAALVH
                     DILMTVGFYAIVGREVSLITVVAVLTVLGYSIYDTIIIFDRIRENAPTVGYNRRRFDE
                     MINRSIRQVVRRSIYTSIFTLIPITALLIFGEATLSDFAFALLVGIVAGTYSSIFIAS
                     PVLSLYKAWRAGKLQAARST"
     gene            complement(2773..>3366)
                     /locus_tag="VFQ10_07495"
     CDS             complement(2773..>3366)
                     /locus_tag="VFQ10_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HET9965222.1"
                     /translation="VISETTVGPTLGLSSLKSGLLASLVGFGLVLLLLLVIYRALGIV
                     ADVALLIYAFLLWGLIVIIPITVTLPGIAGIVLSIGVAADANVVIFERIKEEIRRGKT
                     PRSAIQAGYDKGFRAILDGNVTTLITAFILFALSSGSVRGFAVLLAMGVVLSMFTAIV
                     ITRALLGLLSDRGIHLSPALMGVSKASISATQQARVR"
BASE COUNT          647 a         1066 c         1070 g          583 t
ORIGIN      
        1 agcagcctga tctgctcggg atagtcctcg aagtagcggg cgtactccag ggcgcgctcc
       61 tcggcgacct cgatcacggc ctctggactg tagcgtccgg agaggagcct ctgtgcgtag
      121 ggcctggcga cctcgtagac gttgatggtg gggtctatgg agcgggcgag tccttctgcg
      181 gtcacgagcg ccttggtgag cagggggaag acgggcggca tcctgagcct gtagcgacgc
      241 gcgagcgcta cgagctctga gagcgcctgg cccagggtga cttcgcccac ggagagcccg
      301 gagtacttgt tgaggaactc ccgcaggtcc ctgatgaggt cgctgcgcac ctcggtcgcg
      361 tagcgtacgc cgagccgttc gagggccagc agggcggagg cggcgtcacg ctgtaccacg
      421 gctataaaca gcctcgagag cgcctcgatg tcgccctggc tcatgtagcc gaccatgccg
      481 aaatctagca gggcgaggtc ccccgtcggt gtcaggagca ggttgctagg gtgggggtcc
      541 ccgtggaaga acccgtcctc gaaggccatc ttgaagatcg cctctgcccc catggaggcc
      601 acgcgcctcc gctcagaggg agtgagcacg agcggacgaa tgtccctgaa cctggtcccc
      661 tcgatgtact cctcagtcag caccctcgag gtggagatct ccaggtagac cccagggata
      721 ataacctgcg ttcccgcgaa gttggcggcg aagcggcggg cattctcggc ctcttgtgag
      781 taatccagct cgcgccttat gacgacctcg aactcggcca caaggctgcg cacgtctaca
      841 aagagcctgc gcccgaacct ccggtcgagc aaggcggcga agtcacgcat gagctcgagg
      901 tcgctctcca ccctgcccgg cgcctcgggc cgctgcacct tgatagccac actctccccg
      961 ccacgcagca cggccctgtg gacctgcccg atgctcgccg agccgagcgg cacagggtcg
     1021 aaagaggcga acaactcatc gacgggagcc ccgagctcac cctcgattat ctcctgggcg
     1081 acccctgccg gcatgggcgt agcggtgtcc tggagcttct cgagctcgga gagtactcct
     1141 tcagggagta tgtccgagcg tgtggagaga acctgcccga acttgacgaa ggtcggtccc
     1201 aggtcgtcga gggtgcgccg caatctgaca ccgaagttgg gcgcgagaaa ctcctgcagg
     1261 cccttcttct ccctcctgtc ccgtctgacg tcgaagacga agccgaaacc atggcgcacc
     1321 agaacgcggc tgatctggga gaagcgcctg aggttccccg acggggcggc ccgggagatc
     1381 cctccattcg ccacgcgcgg atccttctgc tacagtcccg gcggcgtctc gggagcatcg
     1441 ccgcgttcgg gcggggactc aggttcgcct gcaggcggag gaggcgccgc gggacggtct
     1501 tcgagcatcc tcaccctgtg ctcgagttgc tcgatgcgga agagaaggtc ctcatagtcc
     1561 tcccttgtcg gaagccctcc tctcctgaga ccctgctgca aagaggcgtc gaccagcgat
     1621 cgcgccccag tcgagcgctc tcgggcgcgg ttcatcacgt cgttgaccac cgtgcggccc
     1681 tgttcgcgct ccacgcgacc ctgctcgatg agagagtcca cgatctcctg caccttgtcc
     1741 cgcgtcgcag cagcggcgcc tatctgcaac aggatcaaac gctccaacgt ctcgccaacc
     1801 gttccgcgca taactacctc cagagtagcg ggtttacttc ctacatgata acgaaggcgc
     1861 aagccgtgtg gccacgccgc taggtagagc gcgcggcctg gagcttgccg gcgcgccagg
     1921 ctttgtagag cgaaagtaca ggtgaggcga tgaagatgga gctgtaggtc cctgcgacga
     1981 tgccgacaag aagggcgaag gcgaagtcgc tcagggtcgc ttccccgaag atcaggagag
     2041 cggttatcgg gatcagggtg aagatggatg tgtagatcga acgcctaacg acctggcgga
     2101 tggagcggtt tatcatctcg tcgaagcggc gcctgttgta gccgacggta ggggcgttct
     2161 ccctgatcct gtcgaagatg attatggtgt cgtagatgga atacccgagc accgtgagga
     2221 cggcgacgac ggtaatcagg ctgacctccc tgcctacgat ggcgtagaaa cctacggtca
     2281 tcagtatgtc gtgaacgagt gcggccatcg cggcgagggc gaaggcgaac tcgaaccgga
     2341 agctgataaa gaccacgatt atgatcagcg cggccaccac ggcctgcaac gcctggtttc
     2401 tgagctgctg gccgaacgcg gggctaacgg tcgtgacgct tacctccccc ccgaacgccc
     2461 ggctcaggga ttcgcgtacc tggctctgct cctcctgaga gcttatgacc ggagtgcgca
     2521 cctcgtagcc atcctgcccc agggtagtga cgacggattc ccctgcaagg tcctctggca
     2581 gcgcggcgcg cacctgctcg tcgctgagct ggcgctctgc gttggcgacg gtgaactgcg
     2641 acccgccctg gaagtctatc ccgaagttga ggccgcccag cgctatggct actgcccctg
     2701 caatcagcaa aacggctgag atcgagaaat acagcttcca gccgccgacg aagtctacct
     2761 tctcgaacac gcctacctca ccctggcctg ttgcgtcgcg gagatacttg ccttggagac
     2821 ccccatcagg gcgggcgaga ggtggatgcc gcggtcagag agcaacccga ggagtgcgcg
     2881 tgtgatcacg atggccgtga acatcgagag cacgaccccc atagcgagca gcacggcgaa
     2941 gcccctgacc gatccactgg agagcgcgaa caggatgaac gccgtaatga gggttgttac
     3001 gttgccgtcc agaatcgccc tgaagccttt gtcgtagccg gcctggatcg cgcttcgcgg
     3061 cgtcttgcca cgcctaatct cctccttgat gcgctcgaag atgacgacgt tggcatcggc
     3121 ggcgaccccg atggagagca cgatgcctgc gatccccggc agcgtcaccg tgatggggat
     3181 gatcacgatc agaccccaca acagaaacgc gtagatgagc agagccacat cggcgacgat
     3241 gcctagcgct ctgtagatca cgagcagcaa cagaagcacg agcccgaacc cgacgagcga
     3301 ggccagaagc ccgctcttca gggaactcag gccgagtgtc ggacctactg tggtctcgga
     3361 gatcac
//
LOCUS       DASTKM010000182         6494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1283962, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000182 DASTKM010000000
VERSION     DASTKM010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283962"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VFQ10_07500"
     CDS             complement(<1..1097)
                     /locus_tag="VFQ10_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET9965223.1"
                     /translation="MFDRVLIANRGEISVRIVRALREMGLTSVAVYSDPDRKALHASL
                     ADEAYHVGPAPAAQSYLNVERLIEVARKCGAEAVHPGYGFLAESAAFARAVEGAGLVW
                     IGPHPRAIEAMGSKVESRRIMAKAGVPMVPGTQDSIGSAKEVSLFGEEHGFPVAVKAS
                     SGGGGKGFALANDASQARSAFERASREGEAYFGDGSVYVEKYLSAPRHVEIQVVRDRH
                     GNAVHLGERDCSIQRRHQKLIEECPSPAIDPKMREAMGGAALAAAEAVDYDNVGTVEF
                     LVEKGGATGEFYFLEMNTRVQVEHPVTEEVTGIDIVKTGIRIAAGEELPFSQRDISWR
                     GHSIEVRLNAEDASNDFAPSPGVVTAYEEPGG"
     gene            complement(1098..1304)
                     /locus_tag="VFQ10_07505"
     CDS             complement(1098..1304)
                     /locus_tag="VFQ10_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965224.1"
                     /translation="MKIEPPPTEEEAAAIVVVLERLSEEGQQDSAFLKRSRWRLAGLL
                     GHALEPGIEPDGSLWSYTRWEGMA"
     gene            complement(1301..2875)
                     /locus_tag="VFQ10_07510"
     CDS             complement(1301..2875)
                     /locus_tag="VFQ10_07510"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET9965225.1"
                     /translation="MQDERLGQNTTAGKIEGLGHLREAAAHPAPEQAVERQRERGKLT
                     AGERVELLLDAGTFVELDRYRVHRSYNFGLEENRPLGDGVITGYGEVSGRKVCVFSQD
                     FTVFGGSLGEVYAQKICKVMDLAISTGCPIIGINDSGGARIQEGVVSLAGYADIFHRN
                     VLASGVVPQISVIVGPCAGGAVYSPAITDFVFMVEETSHMFITGPDVIKSVTGEDVSF
                     EDLGGAATHNTRSGVAHFSAPDEETCFDDVRYLLSFLPENNLESAPYFRPTDDPERMD
                     EDLARLIPDSPQRPYDMRGAIEGVVDDGEFFEVQEGWAQNLVVGFARLDGRSVGVVGN
                     QPSVLAGTLDINASVKGARFVRFCDAFNIPLLTFVDVPGFLPGIDQEWGGIIRHGAKL
                     LYAFSEATVPKMTVITRKAYGGAYDVMCSKHIGADVNVSWPTGEIAVMGASAAVGVIF
                     RRQISEAEDPDAKREELIEDYEEQFNNPMVAAEMGFIDDVIDPRETRPYLIRALQMIR
                     TKRPDRPPRKHGNIPL"
     gene            3015..3587
                     /locus_tag="VFQ10_07515"
     CDS             3015..3587
                     /locus_tag="VFQ10_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9965226.1"
                     /translation="MRRRAVTSDDAKIAERAKGGDTAAYEKLVRGYQEVAFRTAYLIT
                     GDASEAEEATQEAFVKAYRALGRFRSGARFRPWLLAIVANKARNQRRAATRRAGLFLR
                     VAREDTPENSPSSPEAAVLVAERREELLEALRGLREEARWAIACRYFLGLSEEETAAI
                     LGCARGTVKSRLSRAMKGLRAEIAKEGDAG"
     gene            3577..4356
                     /locus_tag="VFQ10_07520"
     CDS             3577..4356
                     /locus_tag="VFQ10_07520"
                     /inference="COORDINATES: protein motif:HMM:NF025643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HET9965227.1"
                     /translation="MQDRELERELRELGSLIDYPPTPNVARAARNVLDEAANEQSRRL
                     RRAFPTMRWAAVAAAFVLVVAVPTLSPGLRATVSDWFVAEDFQSTGVDAGSPERQSEA
                     GAPAAGVSKSATTPAQAPQFSGERISVREAQARMDGTLLLPRTPKLGKPEEIYTVGTA
                     SKEGVMLVYKYGLPPLGDTGIHLVLTEVPGDLEPAYLTGRTTAGSKFDRVSVDGNPGY
                     WRSAGNRLPGQTLLWEQGGVALRLEANVKKEQAIRIAESAR"
     gene            4577..5500
                     /locus_tag="VFQ10_07525"
     CDS             4577..5500
                     /locus_tag="VFQ10_07525"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HET9965228.1"
                     /translation="MEAVRSAIVAKSLGASALVLLVLALAACGSGETKSGGEGVYPAR
                     EPAGVDSAAGSGPDLAQSSEGAVSVTEDPAALGDFGRKIVKTADLGLRSEEVRQSAAR
                     AQQVAATAGGTVLSSQVYRSDDSVTADLVLSVPSEEFERVLDELRSLGEKVTTDSISG
                     QDVTEEYVDLKSRERNLRATEESLLRLYDRAENVEEALSIQRELTTVRGEIELVQGRI
                     KYLDQRSAYSQITLNIQPVTSPPPPKPTWDPGEVVERAWDASLSVLQVMATAIISAVV
                     FGWWLAPVLIGGLLWWRRRNRGSRPAATEPS"
     gene            5606..>6494
                     /locus_tag="VFQ10_07530"
     CDS             5606..>6494
                     /locus_tag="VFQ10_07530"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET9965229.1"
                     /translation="MRVFEKLAEYRYEQLVFCHDKATGLRAIVAIHDTTLGPALGGCR
                     MYPYAGEDDAIVDVLRLARGMTYKAAASGLNLGGGKSVIIGDPHTDKSEALFRSFGRY
                     IETLGGRYIVAEDVGTSTEDANFIRVETSHVVGVDVTRGGSGDPSPLTALGVLQGMRA
                     CVEEVFGTTSLEGRVVAVQGLGHVGYHLCRLLHEEGANLIVTDVHEAAVERVVKEFGA
                     KAVEPDEILPIPCDIFAPCALGAVLDDETIPKLRCRIVAGSANNVLAESGHGEDLSER
                     GILYAPDYVINAGGLINVAD"
BASE COUNT         1182 a         2041 c         2115 g         1156 t
ORIGIN      
        1 ccgcctggtt cctcgtaagc cgtaacgaca ccaggactcg gggcgaagtc gttcgacgcg
       61 tcctcggcgt tcaggcgcac ctcgatggag tggccccgcc aagagatgtc tctctgcgag
      121 aagggaagtt cctcgccagc ggctatacgg atgccggtct tcacgatatc tatccccgtc
      181 acctcttcgg ttacggggtg ctctacctgg acgcgggtgt tcatctctaa gaagtagaac
      241 tcgcctgtgg ctccgccctt ttcgaccagg aactcgacgg tgccgacgtt atcgtagtcg
      301 acggcctcgg cggcggcgag ggcggccccg cccatcgcct cgcgcatctt agggtctatg
      361 gcaggagaag ggcactcttc gatgagcttc tggtggcggc gttggatcga acagtctctc
      421 tcaccgaggt ggacagcgtt cccgtgcctg tcccgaacga cctgtatctc aacgtgacgg
      481 ggagcggaga ggtacttctc gacgtagacc gaaccgtccc cgaagtacgc ctcgccctcc
      541 ctgctcgccc gctcgaatgc gctccttgcc tgggaggcgt cgttggcaag ggcgaagccc
      601 ttgccgcctc ctcccgagga ggccttcacg gccacaggga agccgtgctc ctcaccgaag
      661 aggctgacct ccttcgcgga gccgatagag tcttgcgtgc caggcaccat cgggacaccc
      721 gctttcgcca tgatccttcg gctctccacc ttgctcccca tcgcctctat ggccctcgga
      781 tgaggaccga tccagacgag tccggcccct tcgaccgccc gcgcgaaagc cgcgctctcg
      841 gccaggaaac cgtatcctgg atgcaccgcc tcggccccgc actttcttgc gacctcgatc
      901 agcctctcca cgttcaggta gctctgtgcc gctggcgccg ggccgacgtg gtaggcctcg
      961 tctgcgaggc tggcgtgcag ggctttcctg tctgggtccg agtagactgc gacgctagtt
     1021 agtcccatct cgcgaagcgc gcgaacgatg cggacggaga tttcaccccg gttggcgata
     1081 agcaccctgt cgaacaccta ggccataccc tcccacctgg tgtaggacca tagggagcca
     1141 tctggctcta tcccaggctc tagtgcgtgc ccgagaaggc cggcgagccg ccaccggctg
     1201 cgcttcaaga aggcgctatc ttgttgccct tcttcagaga gcctctccag cacgaccact
     1261 atagcggcgg cctcctcctc ggttggaggg ggttcgatct tcaaagcggg atgtttccgt
     1321 gcttgcgggg cggcctatcg gggcgcttgg tccggatcat ctggagggcc cgaatcaggt
     1381 atggacgtgt ctcccgcgga tctatgacgt cgtctatgaa gcccatctct gcggcgacca
     1441 tagggttgtt gaactgctcc tcgtagtcct cgatgagctc ctcgcgtttc gcatccgggt
     1501 cttcggcctc cgatatctga cgcctgaaga tgacgccgac ggccgctgac gcacccatga
     1561 ccgcgatctc acccgtgggc cacgagacgt tcacgtcggc cccgatgtgc ttggagcaca
     1621 tgacgtcgta tgccccgcca taagccttcc tggtgatgac cgtcatcttg ggcaccgtgg
     1681 cctcagagaa tgcatagagt agcttcgccc cgtggcggat tatgcccccc cactcctggt
     1741 cgatgccagg aagaaaaccg ggcacgtcaa cgaacgtaag aagcgggatg ttgaaggcgt
     1801 cgcagaagcg gacgaacctg gcgcccttca cgctcgcgtt gatgtcgagc gtcccagcga
     1861 gcacggaagg ctggttccct acgacgccga ccgaccgccc atcgagcctg gcgaagccga
     1921 ccaccaggtt ctgggcccaa ccctcttgaa cctcgaagaa ctccccgtca tcgaccacgc
     1981 cttctatcgc gccgcgcatg tcgtaggggc gctgggggga gtcggggatc agacgcgcca
     2041 ggtcctcgtc catccgctcg gggtcatcgg tcggcctgaa gtaaggggcg ctctccaggt
     2101 tgttctcggg caggaaggag agcaggtagc gtacgtcgtc gaagcaggtc tcctcgtcgg
     2161 gagccgagaa gtgggcgacg cctgatctgg tattgtgggt ggccgcgcca ccgaggtcct
     2221 cgaaggaaac gtcttcgccc gtgacgctct ttatgacgtc ggggcccgtg atgaacatgt
     2281 ggctcgtctc ctccaccatg aagacgaagt ccgtgatcgc cggcgagtac accgcgcccc
     2341 ccgcgcaggg accgacgatg acggagatct ggggcaccac gcctgaggcg agcacgttgc
     2401 ggtggaagat atccgcgtag ccggccagag aaacgacgcc ctcctggatg cgcgcccctc
     2461 ccgagtcgtt gatcccgata atgggacatc ccgtcgagat ggcgaggtcc atgaccttgc
     2521 agatcttctg cgcgtagact tcgcccagag aacccccgaa cacggtgaag tcctgggaaa
     2581 agacgcagac tttgcgccca gagacttcgc cgtagcccgt gatgaccccg tctcccagtg
     2641 gcctgttctc ctccaggccg aagttgtaag agcggtgcac cctgtagcgg tccagctcga
     2701 cgaacgtgcc agcgtcgagc aagagctcga cgcgctcgcc ggccgtcagc ttgccgcgtt
     2761 cgcgctgcct ctcgacggcc tgctcggggg cagggtgggc cgccgcctca cgcaggtgcc
     2821 ccagcccctc tatcttgcct gctgtggtat tttgcccgag tcgttcatcc tgcatgggca
     2881 ggcattctag cacgcaagga aatgggggtt tctcgggtta ggcgctcgca gagactcgcc
     2941 tgagacgtca tcattagccg tcacgccggg aaccttttcg cgcccggcgt tgtagatgga
     3001 agtagagagc gacgttgagg aggagggccg tcacctcgga tgacgcgaag atcgcagagc
     3061 gggcgaaggg tggagacact gcggcgtatg agaagctggt gcgtggctac caggaggtgg
     3121 ccttccgtac cgcttacctc atcaccggcg acgcctctga ggccgaggaa gcgacccagg
     3181 aggccttcgt gaaggcttac cgcgccctgg gacgcttcag gtcgggcgcc cgtttcagac
     3241 cctggctgct cgcgatagtg gccaacaagg ccagaaacca gcgcagggcg gccacacgtc
     3301 gcgcaggtct gttcctacgg gtggcgcggg aggacacgcc ggaaaatagt ccttcatcgc
     3361 ccgaagcagc ggtcctggtg gcggagcggc gggaagagtt gctcgaggcg ctgcgtggcc
     3421 tgcgtgagga ggcgaggtgg gccattgcct gccgctactt cctaggactc tccgaagagg
     3481 agacggccgc gatcctgggt tgcgcgcgag gaaccgtcaa gtcacggctc tcgcgggcca
     3541 tgaagggcct gcgcgcggag attgcgaagg agggagatgc aggatagaga actcgagaga
     3601 gagctgaggg agctcggatc tctaatcgat taccctccca cgccgaacgt cgcgcgcgcc
     3661 gcgcgtaacg tgctcgacga agcagccaac gaacagtccc gaagattgag gagggctttc
     3721 ccgactatgc ggtgggcggc ggtagcggcg gcgttcgtgc tcgtcgtcgc cgtcccgacc
     3781 ctctccccag gcctgcgcgc cacggtcagc gactggttcg tggcggagga cttccagagc
     3841 accggtgtcg atgccggctc acccgagaga cagtcggagg caggcgcacc cgccgctggc
     3901 gtctcaaaga gtgccacaac cccggcgcag gctccacagt tctcggggga gcgaatcagc
     3961 gtgcgagagg cgcaggccag aatggatggc acgctcctcc tgccccggac gccgaagcta
     4021 ggaaagccag aggagatcta cacggtcgga accgcaagta aggaaggcgt catgctcgtc
     4081 tacaagtacg gccttccgcc actaggagac accgggatcc acctcgttct gaccgaggtc
     4141 ccaggcgatc tcgaaccagc atatctcaca ggaagaacca ctgccggttc gaagttcgac
     4201 agagtgagtg tggacggaaa tcctggatac tggagatctg ccggaaaccg ccttcccggc
     4261 caaaccctgc tctgggagca agggggcgtg gcgctccggc tcgaggcgaa cgtgaagaag
     4321 gagcaggcga tccgcatcgc cgaatcggcg cgctgataga gggtcacccc ccaaccctcg
     4381 ctggccagca cttcagcttg tcagcttttt gtttgctgac tggctgatgg ctcgtcgagc
     4441 ccaaggctca tgccttggcc cggtgctgtg agattaggga accgatcggg gcctggcagt
     4501 gtacagggag taggttcggc cccgtccgag cgggaaggtg ctccgcgagg gtacacgtac
     4561 gagagaggtg gatggcatgg aagctgtgcg ttctgcaatc gtggcgaaat cactgggggc
     4621 gagcgcgctc gtgctcctcg tgctggccct cgcggcgtgc gggagcggcg agacgaaatc
     4681 gggcggggaa ggagtatacc cagcgcggga gccggcaggt gtggactcag ctgccgggtc
     4741 tggaccagat ctcgcgcagt cctccgaagg tgcggtcagc gtaaccgaag atcctgccgc
     4801 tctcggagat tttgggcgca agatcgtcaa gaccgccgat ctcggcctcc gctcggagga
     4861 ggtccgccag agcgccgcgc gggcgcagca ggtcgccgcg accgccggtg gcaccgttct
     4921 cagctcgcag gtctacagga gcgatgactc cgttacggca gacctcgtac tctcggtccc
     4981 ctccgaggag ttcgagaggg tgctcgatga gttgaggagc ctgggcgaga aggtcacgac
     5041 cgactccatc agcggccagg atgtgaccga ggaatatgta gatctcaagt cccgcgagcg
     5101 caaccttcgg gccaccgaag agagcctgct gaggctctac gacagggctg agaacgtcga
     5161 ggaggcgctc tcgatccagc gcgagttgac gaccgttcgg ggggagatcg agctcgtgca
     5221 gggcaggata aagtacctgg atcagcgtag cgcctactcc cagatcaccc tgaacatcca
     5281 acccgtcact agcccgccgc cacccaagcc tacctgggac cctggagaag tcgtggagcg
     5341 ggcatgggat gcttcgctga gtgtcctcca ggtcatggcg acggctatca tctcggcggt
     5401 cgtcttcggc tggtggctcg cacccgtgct cataggtgga cttctgtggt ggaggaggcg
     5461 gaaccggggt tcgagacccg ctgctacaga gccttcgtag gcccgtcgat accgccacga
     5521 gactttcgag gtcccggtct cgagaacctg agggatagta gaataatgtt ctgaggaacc
     5581 tctggctcgg gaaaggagac agggcttgcg agtattcgag aagctggcgg aataccgcta
     5641 cgaacagctc gtgttctgcc acgacaaggc gacggggcta cgggctatcg tcgccatcca
     5701 cgacaccacg ctcggtcctg ccctaggcgg atgcaggatg tacccctacg ccggcgagga
     5761 cgatgccata gtggacgtac tacggctggc gagagggatg acttacaagg ccgccgcgag
     5821 cggactcaac ctcgggggcg ggaagtcagt cattatcggg gatccgcaca cggacaagtc
     5881 cgaggcgctc ttccgctcgt tcgggcgcta catcgagacc cttggcggcc gctacatcgt
     5941 ggccgaggac gttgggactt cgacggagga cgccaacttt atcagggtcg agacctcaca
     6001 cgtagtcggg gtcgacgtca cccgcggcgg ttcgggcgac ccatcgccac tcaccgccct
     6061 cggggttctg caggggatgc gggcctgcgt cgaggaggtc ttcggcacga cctcactgga
     6121 aggccgggtc gtcgccgtgc agggcctggg gcacgtgggc taccatctgt gccgccttct
     6181 gcacgaagag ggcgctaacc tgatcgtgac tgacgtgcac gaggcggccg tggaacgcgt
     6241 ggtaaaggag ttcggcgcca aggcggtcga gcccgacgag atcctgccca tcccttgcga
     6301 catcttcgcc ccttgcgctc tcggcgccgt cctcgacgat gagaccatac caaagttgcg
     6361 ctgccgcatc gtcgcaggga gcgccaacaa cgtgctcgcc gagtcgggcc acggcgagga
     6421 cctctccgaa cgcggcatac tctacgcccc tgactacgtc ataaacgccg gcggccttat
     6481 caatgttgcc gacg
//
LOCUS       DASTKM010000183         5566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1107801, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000183 DASTKM010000000
VERSION     DASTKM010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107801"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..909)
                     /locus_tag="VFQ10_07535"
     CDS             complement(67..909)
                     /locus_tag="VFQ10_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9965230.1"
                     /translation="MTRRSRSHLPAVLVLAFVVAVTLVLLVGCSAGTDESFEGAAPGE
                     TTGPAGIVGGKITVASNIAYPPFESSPKGGPRGFDIDLMNEIAKRAGFEVEYENVRFD
                     TILRGLDTDLFDAAISAMTITEEREQQIDFSDPYFNADQALLVASDSEVQSIDDLAEA
                     PVGVQAGSTGQIKAEDLLEDEQIGEIVPYRTIGDAFVALEVGKVEGVIYDLSAAHRKI
                     VESGGEIRFVEPISTGEQYGIAFPKDSPLVDPVNQALAEIKDDGTYEKLYKKWIGMPP
                     EDIP"
     gene            1169..2451
                     /locus_tag="VFQ10_07540"
                     /pseudo
     CDS             1169..2451
                     /locus_tag="VFQ10_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
     gene            2605..3252
                     /locus_tag="VFQ10_07545"
     CDS             2605..3252
                     /locus_tag="VFQ10_07545"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET9965231.1"
                     /translation="MESLFDTAEGGEDRERRLEELARQVSVCTKCDLAKTRTNTVFGT
                     GDPYSPLMLVGEGPGENEDATGLPFVGRAGKLLDDILAAVDLTREQIYLTNVVKCRAA
                     VEEGGRLRNRQPKTTEIRACNPYLQGQIEAVKPEIILCLGGPAAKTIIDKDFRITKDR
                     GVWYEIDGIKTMATFHPAYVLRQRGDDLNNAKRLVWKDIRAVYAEYQKAMEARES"
     gene            3382..4443
                     /locus_tag="VFQ10_07550"
     CDS             3382..4443
                     /locus_tag="VFQ10_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HET9965232.1"
                     /translation="MVSTTTGSRMMDSTDKEILNRIQREVPLEREPFEAIGRELGIAG
                     DEVIRRIEALKRVRVIRQISAIFDTRVLGYESSLVAARIQAHKLGEGAKAINSHPGVS
                     HNYERNNEFNLWYTVAVPPDSRLGLEGTVDVLHRISGAEKTRILPTLKLFKIGVTLDM
                     NAGATAKKEAPQYGESDRESADRNISEDDKAAIRALQEDVPLTPRPFDLWGRQVGLSY
                     EELLERAYDLRDRKIMRRFSAVLYHRKAGFRANAMGVWRVPDERIDEVGNAFAHYQAV
                     SHCYQRPTYEDWPYSVFSMVHGRSVEECENVLKAMAEETGLSDYTSLYSTREYKKTRV
                     RYFTPEMEAWERLYAGILR"
     gene            4487..5224
                     /locus_tag="VFQ10_07555"
     CDS             4487..5224
                     /locus_tag="VFQ10_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9965233.1"
                     /translation="MSAALLVALFAALLAGIVTGLTGFGLALISTPILLFVYEPRTVI
                     VLTAIFSIVINSAVVWDSWQEARKRLALALLIPALFGIVAGVEVLSVINPDYVRLGVG
                     VIVIFSALLLVRDMRLPGAGTRWGTLVAGSASGALSTSTGLAGPPIVLLLASRDLPKH
                     EFRGTSALYFLPMSVVGLVVLAFRGLVEAWEVPLGLLLIPAAIAGKALGTALLARVSE
                     RTFRALTLGLVILTGTLGVMTAALALL"
     gene            5287..>5566
                     /locus_tag="VFQ10_07560"
     CDS             5287..>5566
                     /locus_tag="VFQ10_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9965234.1"
                     /translation="MMARVGVQLQPQHASYAQMRDAWLRVEEMGADTIFTWDHFFPLY
                     GDPAGKHFECWTFLGAMAEVTERVEFGALVTCNSYRNPNLLADMARTVD"
BASE COUNT         1022 a         1753 c         1780 g         1011 t
ORIGIN      
        1 gcaaaggcgt aagggcgtag cgtgctgaga gcgaaagccg aaggctggag cgggctgaca
       61 agctctctac ggtatgtcct cgggaggcat cccgatccac ttcttgtaga gcttctcata
      121 ggtgccgtcg tccttgatct cggccagggc ctggttgacg ggatcgacga gcgggctgtc
      181 tttggggaac gctatcccgt actgctcacc agtcgagatg ggttcgacga acctgatctc
      241 cccgccgctc tctacgatct tcctgtgcgc ggccgagagg tcgtagatca ccccctcgac
      301 cttccctacc tccagcgcca cgaacgcatc ccctatggtt ctgtaaggca cgatctcccc
      361 tatctgctcg tcctcgagta gatcttcggc cttgatctgg cccgtcgacc cggcctggac
      421 gccgacgggt gcctcggcga ggtcatcgat agactggact tccgaatcgc tcgctaccag
      481 gagcgcctgg tcggcgttga agtaaggatc ggagaagtct atctgctgct cgcgctcttc
      541 ggttatggtc atcgccgaga tcgccgcgtc gaagaggtcg gtgtcgagtc cccgcaggat
      601 agtgtcgaac ctgacgttct cgtattcgac ctcgaagccg gcccgctttg cgatctcgtt
      661 catgaggtct atgtcgaaac ccctcggtcc gcctttggga gaggattcga acggcgggta
      721 ggcgatgttg gaggcgaccg tgatcttccc gcccactatc cccgctggac ccgtggtttc
      781 gccaggggcg gctccctcga aggattcgtc ggttcccgcg ctgcagccga ccaggaggac
      841 cagcgtaacg gctacaacaa atgccaggac gagcaccgcc ggaaggtggc tcctcgaccg
      901 tctcgtcacc gtgaacttac cctcctgttt tctgccctct agtctaaccc ggccgcggtg
      961 gaaagggtaa cagcggccgg acccggccgc cggcgagtta gctgggtacg acatctcttt
     1021 gcacggggtc ctccctctcg aagccccgct ccaaaatgac catactaatt acgcatcggc
     1081 atctggtcaa gcaggccggg cgttgcctgg aaaagagcct catgctacgg tgccgcgtta
     1141 ctgggaacag gaagggagga cgcgacacat ggtagagttc gatgacggac tctcacagac
     1201 catacgcggt gctgtacatg ccaggcgcga ggacgcggtg agcctgctgc gggagcttgt
     1261 gcgcgtccct tctgttacgg gcgcagaggg tgctgtgggc gcggtcgtcg ggcgcgcctt
     1321 ctcggagcgc ggcctcgacg tggatacgtg ggaggctacg cgcgaggaga cggaaccgta
     1381 ccgcgatcac gtcggggaac agatctcttt cgagaatcga ccgaacctcg ccggcacaag
     1441 gagagggaca ggggacggac gctccatcct gctcaacgcc cacaccgaca ccgtggatcc
     1501 gggcgacccg agcgcgtgga gaggggatcc actctcgggc aacctggagg gagatctgct
     1561 ctacggacgc ggctcttgcg acatgaaggg cggactcgtc acccacctcg tcgcgctcga
     1621 cgctctctgt gacctcggtc tcgggctgcg cggcgacgtc accgtggcag cgactgtagg
     1681 ggaggagaac ggggacttgg cgcactctcg accgtcctgc gcggctatcg cgccgacgcc
     1741 gccatcatca ccgagcctac ccgtctcgcc ctcgtgccgg cccagggtgg ttcgctcgtg
     1801 ttcaggttga agatccgcgg ccgctcggcg cacgccgccg tccgcgacga gggtgtctcg
     1861 gcgctggaga agttcgtacc catcttcgag aacctgcgcg agctggagca ggagcgcaac
     1921 ggcgtcctca gccacccact ctacgacgga ataggcaaca aagtgcccat caacataggc
     1981 gtcgtaaggg ccggcagttg ggcctcgacc gtccccgagt ccctcgttgc ggagggccgc
     2041 gtcggcctga tacctggcga ggaagtagat tccttcaagg atctcgtctc cgaacgtatc
     2101 gccaccgtgg ccgaacggga cccctggctg cgcgagcatc ctcctgagct ggagtggttc
     2161 ggcggccaat tcgcacccgc cgaagtcgag ccgaacgccc cgatctgcga ggccgtgaag
     2221 aaggcccacg agcgggtgac gggcgagacg ccagccgtgg aaggcgtcgc ctacggtgcc
     2281 gacatgcgca tctttataca cttcgggggc atgccttgcg tgatgtacgg cgccggggac
     2341 gtgaacgtgg cgcacgcccc ggacgagcac atcagcatca ccgagtttct gaccgcggcc
     2401 actacggtcg cgtgcctcct cgtcgactgg tgcggcgtgg acgagggcta gagtagtcag
     2461 ggagcgttac accgcgagct cctcttccgc tgcaaaaact gtcggcattg tgggccatcg
     2521 accaggccgg agcaactgct cccctgagtc cctagtcgtg cggtgtagaa tagcctgcag
     2581 gcacgctaac atcacggagg agccttggag agccttttcg atactgctga gggtggtgag
     2641 gaccgcgaga gacggctgga ggaactcgct cgtcaggtct ctgtgtgcac caagtgcgac
     2701 ctggctaaga cccgcaccaa cacggtcttc gggaccggag atccctactc gcctcttatg
     2761 ctcgtcgggg agggccctgg cgagaacgaa gacgccacgg gactgccttt cgtcgggagg
     2821 gcgggcaagc tcctcgacga catcctggcg gccgtggacc tgacccgtga gcagatctac
     2881 ctgacgaacg tcgtgaagtg cagggccgcc gtcgaggagg gcggcaggct gcgcaaccgc
     2941 cagccgaaga ccacggagat aagggcctgc aacccctacc tgcagggcca gatcgaagcc
     3001 gtcaagccag agatcatcct ctgcctcggc ggtcccgccg cgaaaacgat catagacaag
     3061 gatttcagga tcaccaagga tcgaggcgtg tggtacgaga tagacggcat caagacgatg
     3121 gcgaccttcc atcccgccta cgtcctcagg cagcgtggcg acgacctgaa caatgccaag
     3181 cgcctagtct ggaaagacat ccgagccgtc tacgccgaat accagaaggc aatggaggcg
     3241 cgagagtcgt aagggcgggc gagctttgcg tagccctgct aataaggacg aaacaacgta
     3301 aatattctca taccgtgtct tggtcgcgcc tgctatagtg cgttcttgta gaaggcaatc
     3361 gtcgagaagg gctcgtaaag cgtggtcagc acaactaccg gcagtcgcat gatggacagc
     3421 accgacaagg agatactcaa ccggatccag cgcgaggtcc cgctggagcg ggagcctttc
     3481 gaagccatcg ggcgcgagct ggggatcgcg ggcgacgagg ttatccggcg tattgaggcg
     3541 ctcaagagag tgcgggtgat acgccagatc agcgctatct tcgacacgcg cgtgctcggc
     3601 tacgagtcga gcctggtcgc cgccaggatc caggcacaca agctaggcga aggcgccaag
     3661 gctataaact cccaccccgg cgtctcccac aactacgagc gcaacaacga gttcaacctc
     3721 tggtataccg tcgccgtccc gcccgactcc cgcctgggac tcgaaggcac cgtcgacgtg
     3781 ctgcaccgca taagcggcgc cgagaagacc cgcattctgc ccaccttgaa gctcttcaag
     3841 atcggggtga ccctggatat gaatgccggc gctacggcta agaaagaggc tccccagtac
     3901 ggcgagtccg accgggagag cgcagacagg aacatctcgg aagacgataa ggccgccatc
     3961 agggccctcc aggaggatgt ccccttgacg ccgcgtcctt tcgacctgtg gggaaggcag
     4021 gtcggcctct cctacgagga gctcctggag agggcctacg acctgcgtga ccgcaagatc
     4081 atgcgccgct tctcggcggt cctctaccac cgcaaggccg gcttcagggc caacgcgatg
     4141 ggggtctgga gagtgcctga cgagcgcatc gacgaggtgg gcaacgcctt cgcccactac
     4201 caggccgtct cccactgcta ccagcgaccg acctacgaag actggcctta ctccgtcttc
     4261 tccatggtcc atggccgcag cgtcgaggag tgcgagaacg tcctcaaggc gatggccgag
     4321 gaaacgggac tttcggacta cacctccctc tactccaccc gcgagtacaa gaagacacgg
     4381 gtgcgctact tcacgcccga gatggaggcc tgggagcgcc tctacgcagg catactgcgc
     4441 tgattcaggt atcaggcgtt aggcttcggg gtaggaccct tccttgttga gcgcggcgtt
     4501 actcgtggct ctcttcgcgg cgttgctcgc cgggatcgtc accgggctta cgggcttcgg
     4561 gctggccctg atcagcacgc ccatactcct cttcgtctac gagccccgta cggtcatcgt
     4621 gctgacggcc atattctcga tcgtcataaa cagcgccgtg gtatgggatt cctggcaaga
     4681 ggcgcgcaag cggctcgcgc tggcgctcct gatccctgcc ctcttcggca tcgtggccgg
     4741 cgtcgaggtc ctgagcgtga tcaaccctga ctacgtgcgc ctcggggtcg gcgtgatcgt
     4801 catcttctcg gccctgctcc tggtcaggga catgaggctt cctggggccg gcacgcggtg
     4861 ggggactctc gtcgccggtt cggctagcgg ggcgctctcg acctcgaccg ggctcgctgg
     4921 tcctcccata gtgttgctcc tggcctcgcg cgaccttccg aagcatgagt tcagggggac
     4981 gagcgcgctg tactttttgc ccatgagcgt cgtcggactc gtggtcctgg ccttccgcgg
     5041 cctggtggag gcctgggaag ttcccctggg gctcttgcta atccctgccg ccatagccgg
     5101 aaaggcactc ggcacggcgc tcctcgcgcg tgtctccgag aggacgtttc gggcactaac
     5161 tctggggctc gttatcctga ccggcaccct gggcgtcatg accgcggcct tggcgctgtt
     5221 gtagcctgcc tggtcgcatc ctccacgtgg cataatctcc cggttggtcg gaagaggagg
     5281 cgaaggatga tggcaagggt cggggtgcag ctccaaccgc agcacgccag ttacgcccag
     5341 atgcgcgacg cgtggctcag ggtagaggag atgggggctg atacgatttt cacctgggac
     5401 cacttcttcc cgctctacgg cgatcccgcc ggcaagcact tcgagtgctg gacgttcctc
     5461 ggggcgatgg cggaggtcac ggagagggta gagttcgggg cgctcgtcac gtgcaactcc
     5521 tacaggaacc cgaacctgct cgcagatatg gcgcgcacgg tcgatc
//
LOCUS       DASTKM010000184         7516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1459717, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000184 DASTKM010000000
VERSION     DASTKM010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459717"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFQ10_07565"
     CDS             <1..205
                     /locus_tag="VFQ10_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965235.1"
                     /translation="RREALVLVVMAGAVGLLYSDGVHSVPPFLWAGSRGVLPPAGPFI
                     FQHHTQAPEALSVEPEELVQVGD"
     gene            594..806
                     /locus_tag="VFQ10_07570"
     CDS             594..806
                     /locus_tag="VFQ10_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965236.1"
                     /translation="MKVRVCEHHRISERRGMVGRIVGRYGGEEYVAVDVRFGDRHYQL
                     FSPKDLEEISSPQPWWRSLLGRESRS"
     gene            809..1030
                     /locus_tag="VFQ10_07575"
     CDS             809..1030
                     /locus_tag="VFQ10_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965237.1"
                     /translation="MKEHWFPKFDYYLDESDPEIAVLCRQDCSFVAAFSASGATREGF
                     VEAAREDYWELVESHTDALGPESQERLSS"
     gene            1151..1297
                     /locus_tag="VFQ10_07580"
     CDS             1151..1297
                     /locus_tag="VFQ10_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965238.1"
                     /translation="MTVLRPRHDYKVFEGRSHYCVIGSEGWKEVADYALEWAVEHAAV
                     RPAV"
     gene            complement(1500..1649)
                     /locus_tag="VFQ10_07585"
     CDS             complement(1500..1649)
                     /locus_tag="VFQ10_07585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HET9965239.1"
                     /translation="MGVATAAGFASLETMGYGFASWLSSKDNLAVLDEVLLVRGLTSP
                     AGHMA"
     gene            complement(1666..1797)
                     /locus_tag="VFQ10_07590"
     CDS             complement(1666..1797)
                     /locus_tag="VFQ10_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965240.1"
                     /translation="MPVTFVAYLYEGLPSWDVPLQTLAGCFLWGGVLGTVFAGALPL"
     gene            complement(2131..3189)
                     /locus_tag="VFQ10_07595"
     CDS             complement(2131..3189)
                     /locus_tag="VFQ10_07595"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HET9965241.1"
                     /translation="MRKLVRGVLLVGSVGLALHLVLPQIPGLERSLRLVAGTSHALVG
                     AAFLAELLSELCYAELLRRSVGVTFTAGSSPSVRRLGRWFMLRLTVTGYGAAHVLPGG
                     GATAAAVTYGALRRKGYDPESVGLALAAVSVLVYGALGMLFSGSLIYMLLDGDLGPVS
                     TAASIFYFALTLSVTIVAYAAYRRPTFARNVAGKGVRLTGRLLGGEGLGRRARMWSAR
                     LISRFGEEIRAARRGLAARPTEAPKLAALALSYWAFDALCLILMFDALGVGADPFVLL
                     VAYGVATTIAALPLTPGGIGVFEVTMLATLALLGVGSEAAIPILGYRLFNFWLPIPLA
                     AVFYPTLRRTGRDLDCAS"
     gene            complement(3239..3880)
                     /locus_tag="VFQ10_07600"
     CDS             complement(3239..3880)
                     /locus_tag="VFQ10_07600"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET9965242.1"
                     /translation="MTVLGELLHLIGRYGYLTVFSGVMLESAGVPLPGETVLIAAGAL
                     VHKGVLDFKDTFFFGILGAVTGDQIGYWVGRFGGRPFLLRWGRYALITPERLGHAEAF
                     FARHGGRAVFLARFVTGLRVVGALVAGTSRMPWGKFVLYNVLGGTMWAVLAVSLGYFL
                     WASISLVEHWVGRASLLLVAALALALLLRWTYRRATRGGRPGGGPRDPENRLS"
     gene            complement(4160..4471)
                     /locus_tag="VFQ10_07605"
     CDS             complement(4160..4471)
                     /locus_tag="VFQ10_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965243.1"
                     /translation="MIARTWTGTVRRSDADEYAEYIRETGFAEYGQTPGNRGAWMLRR
                     DEGERTEFITLSLWESEDAIRSFAGDDIEAAVLYPEDERYLIGGESAVAHYEVVDEVV
                     P"
     gene            complement(4468..5118)
                     /locus_tag="VFQ10_07610"
     CDS             complement(4468..5118)
                     /locus_tag="VFQ10_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9965244.1"
                     /translation="MGTIILSSLISLDGRINGPDGGIGWHMVGPEVHQYFNDQAREVD
                     TFLYGRRTYEGMAEFWPTAERDPDSTPEVIEYAKLARPKPKLVFSNTLESAGWNTEIV
                     DGDHLAETVAELRARPGTKHLLYASADLAATFMRLDLIDEYWLFVTLTVADLVGYPGP
                     ALIMVGDRDEEIPMEHTLTLREVLPDAQLAVLPGAGHGSLDARIVIDFLAGREEET"
     gene            complement(5274..5645)
                     /locus_tag="VFQ10_07615"
     CDS             complement(5274..5645)
                     /locus_tag="VFQ10_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965245.1"
                     /translation="MRGHNPTPAMNLSRAALAFRTFHAAIAIEMLLAIAYVWWCALRG
                     RRDQLLRIAAASLIGEGVVVAANHGDCPLGGLQERLGDPVPLFELVLSPRAAKRAVPT
                     LGAITAAGLALLMVRGRCDAR"
     gene            5957..6358
                     /locus_tag="VFQ10_07620"
     CDS             5957..6358
                     /locus_tag="VFQ10_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965246.1"
                     /translation="MNVYVFGNEYVAEDKRAIEVARELEGAIEGISFVFVNPNEDVPF
                     VDQRLSVILDTVEGIQDVALVEGDRIDGLVLSPRGSVHDFDLAFQLRYLRKLGKLGEF
                     TIIGIPQEGEVDYLRIQSILRKLVAHDMQGS"
     gene            complement(6298..>7516)
                     /locus_tag="VFQ10_07625"
     CDS             complement(6298..>7516)
                     /locus_tag="VFQ10_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HET9965247.1"
                     /translation="VKDLRFIVSDYRRFFTTAVKGRRMIAVPSFLSRICGTCSVAHLF
                     ASLMAIESAQGIAVTEQTRALRRLAYDGLMIRDHALHLYFFVLPDVLGVDSILDIGDD
                     PDDPGHTLLHDSLDIKRLGTDITNTVIGAAIHAPWPTIGGSLKHPDPATFPDLISRLE
                     AMRPQVLRGIETFFGWEASLVRNTDYLALRNDTRFDFLEGDVVNSDGRRIARSGFDNY
                     LHYVQIPHSHAEGYRFSDTGEDYLVGALARLNLNRDILHPRTKGDAETALSAFPSNNV
                     YHNNLAQAIEILQCVDDALDILRTLEVADEKPVRVPAKEGSGVGIIEAPRGLLYHMAN
                     VNEEGLIEDYDVIVPTAQNQINIENDLKDYFNENLDRDEETLRVNAESIVRAYDPCMS
                     CATNFLKIDWIRR"
BASE COUNT         1566 a         2298 c         2269 g         1383 t
ORIGIN      
        1 caggcgtgag gccctcgtcc tggtggtcat ggccggcgcg gtggggttgc tgtattctga
       61 tggtgtccac agtgttcccc cttttctgtg ggccgggtcc cggggtgtgc taccacccgc
      121 ggggccattc attttccagc accatacgca agctccggag gccctgagcg tcgaacccga
      181 agaactcgtc caggtgggag attaggcatg ggcaagacca agggccgtgc aaacggcgac
      241 ggggacgtgt tcctatgtcc tctatccccc gtgcagttga acttactgtg ctcttgactt
      301 gtggtgaaga atggacttag tcaagccgtt tttcttgctc gaggttgccc gccgtttgct
      361 ggatgtagcg ttctgggcgg tgtcagcaac acgcgttcgg cactactctg cccgaccaag
      421 ttaggacctt ggtcccatga cagcgggact cctcccccct taaggtaatt acgacgaggt
      481 aggagattat cagtcggatg gtggaggtgc ccgacgagaa ggagctcgtc cctgagccct
      541 tctagagcgt tgtggggatg tacgcaccat gagatcggaa gaagcgcgtc ggaatgaagg
      601 taagggtgtg tgagcaccat cggatctcgg agcggcgggg catggtcggt aggatcgtgg
      661 gccgctacgg gggagaagag tacgtagccg tggacgtgcg ctttggagac cgacactatc
      721 agttgttctc gccgaaggac ctggaggaga tctcctcccc tcaaccctgg tggcgctctc
      781 ttctgggtcg cgaaagcagg agctagggat gaaagagcac tggtttccca agttcgacta
      841 ctaccttgac gagtcggacc cagaaatagc agtcctgtgc cgccaggact gctcgttcgt
      901 ggcagccttc agcgcaagtg gggccaccag agagggcttc gttgaagcag ccagggagga
      961 ttactgggaa cttgtcgagt ctcatactga cgcgcttggt ccggagagcc aggagcgcct
     1021 gagctcttaa cctcacgacc ctgcctatcc acccgacgcc cgatgaccac aattctcatg
     1081 gaagtccgta tgcagtatcc tgcataaccc cgtctagggg gacgtctggt gtgcgctctc
     1141 ggactggtga atgaccgtgc ttcggccacg acatgactac aaggtgttcg agggacgctc
     1201 ccactactgt gtcattggga gcgaaggctg gaaggaagtg gcagactacg cgctcgagtg
     1261 ggccgtcgag cacgccgcag tacgcccggc ggtatgagca cacctcctgc aactaactcg
     1321 agggtctcaa tgcgacctgc tggggcacca accggcgtag ggctcctgac gctcgcgaag
     1381 gtgtcccaca aagcgtgcag caccaccgcc gtcaagaaag ccccgacaac tcgccagtct
     1441 agggtcgcgt gccccacctt gagtctctct cgccacagga cagcgcacac caaccctgtt
     1501 caagccatgt gaccggcagg tgaggtcagg ccgcgtacca agagcacctc gtccaagact
     1561 gcgaggttgt ccttagagga gagccaggag gcgaacccgt agcccatcgt ctccagactc
     1621 gcgaagcccg ccgcggtagc aacacccaag actatgcctg ccgcctcaga gcggtagcgc
     1681 ccccgcgaag accgtgccca acaccccgcc ccacaggaag caccccgcca aggtctgcag
     1741 agggacatcc cagctcggca gcccctcgta aagataggcc acgaaggtga cgggcaccaa
     1801 gaaagctccg agcaagatac tgggtacata tatagggttc gaagtggcaa atagggttcg
     1861 aagtggcaac gagcgtcagc tcgaggagat acaccaggat caagccgctt acaaagatct
     1921 ggagccacct gcgcctcaga acacccaccg ctcttcatcc tttcctcgtc tcggttctcg
     1981 aatccgaaac acaacgtgct ttttccagtg ccctcgacgg ccgcacaacg aaggtgcaca
     2041 ttgccgaatg cagaacgacc tcacactggt cgctcccgat cttcgcgaac ccgatcctgg
     2101 tagcgcaacc accggcggtt gcccgaagca ctaactggcg caatcgaggt ctcgtccagt
     2161 gcgtcggagt gtcgggtaga aaacagcggc gagcggtatg ggaagccaga aattgaagag
     2221 ccggtacccg aggatcggta tcgcggcctc cgaaccaacc ccgagcaggg cgagcgtggc
     2281 gagcatggtc acctcgaaga cacctatgcc ccccggtgtt agcggtaacg ccgcgatggt
     2341 ggtggccacc ccgtaggcca cgaggagaac gaagggatcg gcccccacgc caagggcgtc
     2401 gaacatgagg atcaggcaca gggcgtcgaa ggcccagtaa ctcagcgcca gcgccgcgag
     2461 cttgggcgcc tctgttggcc gagcagccag accccgtcgc gccgcccgaa tttcttcccc
     2521 gaaccgtgag atgagccggg ctgaccacat tcttgctctt cgccccaacc cttcgccgcc
     2581 aagaaggcgt ccggtgaggc gtactccctt ccccgccacg ttcctagcga acgtgggcct
     2641 ccggtacgca gcgtaagcaa caatggttac ggatagggtc aacgcgaagt agaagatgct
     2701 cgcggccgtg ctcacggggc ccagatctcc gtccaggagc atatagatga ggctcccgga
     2761 gaagagcatc cctagggcac cgtagactag gaccgagact gcggccaacg ccaaacccac
     2821 actttcagga tcgtagccct tgcgcctaag ggcaccgtag gtcacggctg cggccgtggc
     2881 tccaccccca ggtagtacgt gcgccgcccc gtagccggtt acggtaaggc ggagcatgaa
     2941 ccatcttccc agccgtcgga cgcttggcga cgatcccgct gtgaaggtca cgccgaccga
     3001 gcgccttagc agctccgcgt agcacagctc gctcagaagt tccgccagaa acgctgctcc
     3061 tactagggca tgcgaagtgc cggcgacgag ccgcagcgac ctctcgagcc ccggtatctg
     3121 cgggagcacg aggtgcaatg caaggcccac cgagccgacc aacagtacgc cacgcaccaa
     3181 cttcctcacg ttcgaccaac ccgagagttc ccggcgcagc cgcgaccctg cacacctttc
     3241 acgacaacct gttttcgggg tcgcgcggac cgccgccagg ccgtcccccg cgggtagctc
     3301 tccggtacgt ccaacgcagc agtaaagcga gggccagcgc cgcgacaagc aggagagaag
     3361 cacgaccgac ccagtgctca accaggctta tgctcgccca cagaaagtag ccgagcgaga
     3421 cggctaggac ggcccacatg gtcccaccga ggacgttgta gaggacgaac ttcccccagg
     3481 gcatgcggct cgtgccggcg actagcgccc cgacgacccg cagtcccgtg acgaagcggg
     3541 caaggaagac ggccctgcca ccgtgcctgg cgaaaaatgc ctcggcgtgc cccaagcgct
     3601 ccggcgtgat gagggcgtat ctcccccacc gcagtaagaa cgggcgaccg ccgaagcggc
     3661 ctacccaata accgatctgg tcaccggtta cggcgccgag gataccgaag aaaaacgtgt
     3721 ctttaaagtc caataccccc ttatggacca acgccccagc agcgatcagg acggtctcgc
     3781 ccggaagcgg caccccggcg ctctcgagca tcacgccgga gaagaccgtc aggtatccgt
     3841 accggccgat caggtgaagc agctcgccca aaaccgtcac gcagccccga gagcctttat
     3901 agcccgaccc acgagcacag cgacgattag cagcaagtgc agctcctgca tcatcagaac
     3961 cttcgctcgc cgactcagga tagcattgtc ggtggggctg aaggcggcgc tcgggttgaa
     4021 ggcgagggag atatagtcca aaaagtcggg tgatcaggca taggcggacg tgtcgccaag
     4081 gtcaatctgg gaacaggaag ccttcgctaa tatgcccttc cccgcgcccg gtcgcgaagt
     4141 gagggaggcc ggcgcgtgct cacgggacca cctcgtcgac gacctcgtag tgtgccacgg
     4201 ccgactcgcc gccaattaga taacgctcgt cttcggggta aagaaccgcg gcctcgatgt
     4261 cgtccccggc gaaggagcgg atcgcatcct cagactccca cagcgagagc gtgatgaact
     4321 ctgtccgctc accctcgtcg cgccggagca tccacgcacc gcggttcccg ggagtctggc
     4381 cgtactcggc gaagcccgtc tctcggatgt actcggcata ctcgtcggcg tcggatcggc
     4441 gcaccgtgcc cgtccaggtg cgcgcgatca tgtttcctcc tctcgccccg ccaggaaatc
     4501 gatgacgatc ctggcgtcga ggctgccgtg gccggcgcct ggcagaacgg cgagctgcgc
     4561 gtcagggagt acttcgcgca gggtgagggt gtgctccatg gggatctcct cgtcgcgatc
     4621 gccgaccata atgagcgccg gcccgggata gccaacgagg tcggcgaccg tgagggtgac
     4681 gaatagccag tactcatcga tgagatcgag tcgcatgaaa gtggcagcca ggtcggcgct
     4741 ggcgtatagc aggtgtttgg tcccgggccg cgctcgaagt tcggcaacgg tctcggcaag
     4801 gtgatccccg tcgacgatct cggtgttcca accggcactc tcaagcgtgt tagagaacac
     4861 gagcttgggc ttgggtcgag cgagcttggc gtactcgatc acctctggcg tgctgtcggg
     4921 atccctctca gcggtcggcc agaactcggc catgccctcg taggtcctgc ggccgtagag
     4981 gaacgtgtca acttcccgcg cctgatcgtt gaagtactgg tggacttccg gtccgaccat
     5041 gtgccagccg atcccgccgt cgggcccgtt gatgcggccg tcgagggaga tcaagctcga
     5101 gagaatgata gtgcccatgg gtaggccttt cggtcgcctc ccgacgcggc gaggttggct
     5161 agcgtggtgg ctctgactga acacaggatt ctcttgaggc gtaatcgagc cctctctcac
     5221 caacaaacta cgattagaag cgccgcgaag agccatttca cctctgtcac ctcctaacga
     5281 gcgtcgcatc gaccgcgtac catcaacagg gcaagcccgg ctgcggtgat ggcgccaagc
     5341 gtcggcacgg ctcgctttgc tgcgcgtggc gagagtacca gctcaaacag cgggacgggg
     5401 tcgccaagac gttcttgcag tccgcctagc gggcaatctc cgtggttggc agcaacgacc
     5461 acgccctcgc cgatcagcga cgctgcggct atgcgaagca gctggtcgcg gcgacccctc
     5521 aacgcgcacc accacacgta ggcgatggca agcagcatct ctatggcgat agcggcgtgg
     5581 aaggtccgaa aggccagcgc ggctcgcgag aggttcatcg ctggagttgg gttgtgccct
     5641 ctcatctcgg ctacctcctc actcttcttg ccactgcttc tatggtgtac tttccgcgct
     5701 cacttccaca ttagagtcta ctccggagat acctaggtgc cgtgagcaag acagatcaaa
     5761 tgcatagtca aattgtacta gcgctgtttc aggcagcagg cggaatgtcc acatcgatgg
     5821 gtccaactac ccccctccgg ggccacgctt cgacccgcca cgcaccagga gaacgggact
     5881 gcgggcatgt gagacaactc ccacctggat gtgaggcagg ccaaccacac tacaaatcgc
     5941 tgtagaatgg aactaaatga atgtctatgt ttttggtaac gaatatgtag cggaagacaa
     6001 gagggcgatt gaagttgcaa gagagctgga aggtgcaata gagggtatct cgtttgtctt
     6061 cgtcaaccct aacgaagacg ttccctttgt ggaccagcgc ctttctgtga tcctcgatac
     6121 ggttgagggg atccaggacg tggcgctggt ggaaggagac aggatcgatg ggttggtctt
     6181 gtcccctcgc ggatcagtac acgacttcga tctggcattt caactgaggt acttgaggaa
     6241 gctgggcaaa ctgggggaat tcaccatcat cggcataccg caagaaggag aagtcgacta
     6301 tcttcgtatc cagtcgatct tgaggaagtt ggtcgcgcac gacatgcagg ggtcgtaggc
     6361 gcgaacgatg ctctcggcat tcacgcgcaa cgtctcttcg tctctatcca ggttctcgtt
     6421 gaagtaatcc ttcaggtcgt tctcgatgtt gatctggttt tgcgctgtcg gaacgatcac
     6481 gtcgtagtcc tcgatcaggc cttcctcatt gacattcgcc atgtggtaaa ggagtccccg
     6541 cggcgcttct atgatgccaa ctccactacc ttcctttgca gggactcgaa cgggtttctc
     6601 atcagccacc tctaaggttc tcaggatatc caaggcgtca tcgacacact gcaggatctc
     6661 gatcgcctgc gccaggttgt tgtggtacac gttgttcgag gggaaagccg agagagcggt
     6721 ttcagcgtcg cccttcgtcc ggggatgaag aatgtcccga ttcaaattga gtcgtgcgag
     6781 tgcgccgacg agatagtctt cacctgtatc ggagaacctg tacccttccg catgtgaatg
     6841 ggggatctgt acgtagtgca ggtagttgtc gaaccccgag cgggcgatcc gcctaccgtc
     6901 cgagttgaca acgtcgcctt cgagaaaatc gaagcgcgta tcgttgcgga gagccagata
     6961 gtccgtattc cttacgaggc tcgcttccca cccgaagaag gtctcgatcc ccctgagtac
     7021 ctggggtcgc atggcctcaa gacgcgagat aaggtcgggg aacgtcgcag gatctggatg
     7081 cttcagggat cccccaatcg tcggccaagg ggcgtggatt gccgccccga tgacggtgtt
     7141 ggtgatgtcc gtgcccagcc gcttgatatc taaggaatcg tgcaagagcg tgtggcccgg
     7201 gtcgtcggga tcatctccga tatccaagat cgagtccacc ccgagaacat cgggcaacac
     7261 gaagaagtac agatgcagcg cgtggtccct gatcatgagc ccgtcatatg ccaatcggcg
     7321 gagcgctctg gtttgctctg tgaccgcaat cccctgtgcg ctttcgatcg ccattagaga
     7381 ggcgaagaga tgggcgaccg aacacgtgcc gcagatcctg gacagaaacg atggaaccgc
     7441 gatcatgcgc ctgcccttta ccgcggtcgt gaaaaagcgc ctgtagtccg agacgatgaa
     7501 ccgcagatcc ttgact
//
LOCUS       DASTKM010000185         6305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_227616, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000185 DASTKM010000000
VERSION     DASTKM010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227616"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFQ10_07630"
     CDS             complement(<1..719)
                     /locus_tag="VFQ10_07630"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9965248.1"
                     /translation="MTSGNGRATHRFRSRDWFDDPHRRDQSALYLERFMNYGITPEEL
                     RSGRPVVGIAQSGSDLSPCNRHHLELAGRVRDGIRDGGGIPMEFPVHPINENCRRPTA
                     ALDRNLSYLGLVEILYGYFIDAVVLTTGCDKTTPAAIMAASTVDIPAIVLSGGPMLDG
                     YFEGELVGSGMAIWKSRRRLAAGQIDEEQFFETALASAPSAGHCNTMGTASTMNALAE
                     VLGLSLPGCAAIPAPYRERGQ"
     gene            complement(747..1511)
                     /locus_tag="VFQ10_07635"
     CDS             complement(747..1511)
                     /locus_tag="VFQ10_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9965249.1"
                     /translation="MEGTDLLGKTAFVIGASSGIGLATANLFADVGATVHAAARRREA
                     IEDGAGGRNVAAHALDISDKEAVWRAVEEVGEADGIDILVVAAGMNFPERRLEQLTAD
                     GWDAMISVNLSGAFYAIRAALPYLRSSGGLVVLISSVSGRWPDASGPAYQASKAGMVE
                     LAHAAGFEEHVNGVRFTSILPGIVDTPILDNRPEPPPKEVRDAALKPEDIARACLFLA
                     TLPPRAYVPELIMVPTAIQALGKTSTATPPVSQGDE"
     gene            complement(1511..2536)
                     /locus_tag="VFQ10_07640"
     CDS             complement(1511..2536)
                     /locus_tag="VFQ10_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HET9965250.1"
                     /translation="MKGIVWHGPEEMSVEEVPEPAVEPGTVVVRPTATGICGSEIEGY
                     LGRMGNRTPPLVMGHEFAGTVTEVGEGVDEDLLGRLVAVNPLSSDGTCRLCLAGLTNL
                     CPNRRLVGIHSPGGFAEYALAPAANVYPLPDGVEARTGALAEPLANGVHAARLGLADS
                     PVEQTVVIGAGTIGLMCLQAAVLDGIPQVHAVEPVQARREQALALGAGAVHESGEEAR
                     EALEDPTEGLGADLVIDAVGAEVTRRMALELLRPGGRVVYVGLHDDDTTLGFHDVVRG
                     QLDLQGSYAYTAEDYEQALEWLVEGKAGIGELPPVLPLEEGPEAFAELVRGPSAQIKV
                     FLAGSEE"
     gene            complement(2603..3298)
                     /locus_tag="VFQ10_07645"
     CDS             complement(2603..3298)
                     /locus_tag="VFQ10_07645"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9965251.1"
                     /translation="MTSPFAGYEWVDLSHTLEEDIPAYPTHARFGKTLYESYEYGDAA
                     RHYGLTMSEHTGTHMDAPLHFISEGPAHYGTDEIPLGRLAGRAATIGATDLGADDLVK
                     VGYIEAWEEENAPIEAGDRVLIRYGWDLRWATGPEGRRFLEGWPGLSGEAAQYLVGKG
                     VTLVGCDTLAIDAAGSTEHPAHYALLGSEVYIVENLKNLDRLPPFCLFLALPLKFKGG
                     SGSPVRAVALVRP"
     gene            complement(3295..3627)
                     /locus_tag="VFQ10_07650"
     CDS             complement(3295..3627)
                     /locus_tag="VFQ10_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9965252.1"
                     /translation="MNPYARWIQDLPQVDTPFDGLEGHMISGRDGQAVFFRAAERFEV
                     PPHSHGAQWGIVVSGLLHLTIDGEPATYEPGETYDIPGGAEHTAILEAGTCVIDVFQD
                     PGRYSPKE"
     gene            3715..4470
                     /locus_tag="VFQ10_07655"
     CDS             3715..4470
                     /locus_tag="VFQ10_07655"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9965253.1"
                     /translation="MERLSGKRSIVTGAGSGIGRAIALRFAAEGARVVISDVDEEAAA
                     SVAAEAGGETLVRKTDVTSTSEVETLVQTSVEEWGGLDVMVNNAGIGVAGTTTVTTEE
                     DYERVMDVCLRGTFLGMKHAIPAIRDSEGGSVINMSSVAALVGIADRAVYSAAKGAIL
                     AITRAAAIDHVEEGVRVNCIAPGTVDTPWIGRITAGYDDPEEARRNMQARQPHGRLVT
                     PEEIAAMAAYLASDESASVIGACMVVDGGVTAR"
     gene            4467..5120
                     /locus_tag="VFQ10_07660"
     CDS             4467..5120
                     /locus_tag="VFQ10_07660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9965254.1"
                     /translation="MSEAHRRTVESGYDQMAEEYLATKDPEDPLALGALEDLALLLPS
                     DAAVLDLGCGAGVPVTRWLADRGFAVTGVDVSAKQLELARTNVPEGTFLKADMTEVVF
                     APESFDAVLAFHSIIHVPRTEHPTLLESVHRWLEPGGALLATMTVADYEGRDEDWEGW
                     GAPMVWSHYDKNANVAMLRDAGFEIRYAEPRTGGGTADETETWLWVLASKKSREGTT"
     gene            5117..>6305
                     /locus_tag="VFQ10_07665"
     CDS             5117..>6305
                     /locus_tag="VFQ10_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HET9965255.1"
                     /translation="MTTITRVVARDIRFPTSLDLSGSDAMNEAPDYSAAYVVLETDGG
                     LEGHGFTFTIGRGNELCVAAIRALAPLVEGRTLESFTRDMGAFWGHVTGDSQLRWVGP
                     EKGVIHLATAAIVNAVWDLYAKAERKPLWKLLSDMSPEELISCVDFRYITDAITREEA
                     LEILRRNEKTKPDREAEMLEDGFPAYTTSAGWLGYPDEKLRSLVREAVEKGWTHFKMK
                     VGGDIEDDVRRAALIRDEIGPDRTLMMDANQVWGVGQAIENMERLKVFDPWWIEEPTS
                     PDDVLGHARIAEAVSPVGVATGEHCQNRVIFKQLLQADAISFCQIDACRLGGVNEVIA
                     VLLMAAKFGVPICPHAGGVGLCEYVQHLSIFDYIAVGASLEDRILEYVDHLHEHFVDP
                     VEIQ"
BASE COUNT         1117 a         2020 c         2092 g         1076 t
ORIGIN      
        1 atctggccgc gctcccggta gggcgcgggg atggccgcgc accctggcag tgaaagacct
       61 agcacctcgg ccagggcgtt catggtcgag gccgtaccca tagtattgca gtggcctgca
      121 gacggcgcgg aggccagggc cgtctcgaag aactgctcct cgtctatctg gccagcagcc
      181 agacggcggc ggctcttcca tatggccatg ccagaaccta cgagttcgcc ctcgaagtaa
      241 ccgtcgagca taggaccgcc ggagagcacg atggcaggga tgtccacggt cgaggcggcc
      301 attatcgccg ccggcgtcgt cttgtcacag cctgtcgtaa gcaccacggc gtcgataaag
      361 taaccgtaga ggatctcgac gagcccgaga taagagaggt tgcggtctag cgcggcggtc
      421 ggcctgcggc agttctcgtt gatgggatgc acggggaact ccatcgggat tccaccccca
      481 tcgcgtatcc cgtcacgcac acggcccgcc agctccaggt ggtgcctgtt gcagggcgag
      541 aggtcgctgc cgctctgtgc tatgccgact accggcctgc cggagcgcag ctcctctggt
      601 gtgatcccgt agttcataaa gcgctctaga tacagcgcgc tctggtcccg gcgatgggga
      661 tcgtcgaacc agtcgcgcga gcggaaccgg tgcgttgccc ggccgttacc tgaagtcatg
      721 gtagatctcc ttgtcgggtc ttcatcctac tcatcgccct gtgagaccgg aggcgtcgct
      781 gtcgaggtct tgccgagggc ctgtattgcg gtcgggacca tgatcaactc tgggacatag
      841 gcgcggggcg ggagcgtcgc gaggaagagg catgcccttg cgatgtcctc gggcttgagc
      901 gcggcgtcgc ggacttcttt cgggggcggc tcggggcggt tgtccaggat gggcgtatcc
      961 acaatgccag gtaggatcga ggtaaagcgc acgccgttta cgtgttcctc gaagccggcg
     1021 gcgtgcgcca gctcgaccat ccctgccttg gaggcctggt aggccgggcc cgaggcgtcg
     1081 ggccaccgtc cggagacgct gctgatcagg acgacgagac cgccggatga gcgaaggtag
     1141 ggcaatgccg cccggatcgc gtagaaagcg ccgctgaggt ttaccgagat catggcgtcc
     1201 cacccgtcgg ccgtgagctg ctccagacgc cgctcaggga agttcatccc ggcggcgacg
     1261 acgaggatgt ctatcccgtc ggcttcccct acctcttcga cggcccgcca gacggcctct
     1321 ttatccgaga tgtcgagcgc atgagccgca acgttacgcc ctcctgcgcc atcttctatc
     1381 gcctcgcggc ggcgggccgc ggcgtggacc gtggccccga cgtcggcgaa gaggttggcc
     1441 gtggcgagcc cgatgccgct gctcgccccg atgacgaacg ccgtcttccc caagagatcc
     1501 gtgccttcca ctattcctcc gaccctgcta ggaagacctt gatctgcgcc gagggaccac
     1561 gcaccaactc ggcgaacgcc tcaggtccct cctccagcgg caggacgggc ggcagctccc
     1621 cgataccggc cttaccttcg acgagccatt cgagtgcctg ctcgtagtcc tccgccgtgt
     1681 acgcgtacga accctgcagg tcgagttgac cccgcacgac atcgtggaac ccgagggtgg
     1741 tgtcgtcgtc gtgcagccct acgtacacga cccgcccgcc tggccgtagg agttcgaggg
     1801 ccatgcgccg cgtgacctcg gcgccgacgg cgtcgatgac gaggtcagca ccgaggcctt
     1861 ccgtggggtc ctctagcgcc tcgcgcgcct cctcaccgga ctcgtgtacc gcgcccgctc
     1921 cgagggccag cgcctgctcg cggcgcgctt gcacgggttc gacggcgtgt acctggggga
     1981 tgccgtccag gaccgcggcc tgcaggcaca taaggcctat ggtgcccgcc ccgatcacca
     2041 cggtttgctc cacgggactg tcagcgagcc cgagcctcgc ggcgtggacg ccattggcga
     2101 gaggctcggc gagggcgccc gtgcgtgcct cgacgccatc tggaagcgga tacacgttcg
     2161 ccgcgggcgc gagcgcgtat tccgcgaagc cgccagggga gtggatgccg acgagcctgc
     2221 gattcggaca cagattggtc aaccctgcca ggcacaggcg gcacgtcccg tcggaggaga
     2281 gcgggttcac ggccacgagg cggccgagga gatcttcgtc gaccccttca ccgacctccg
     2341 tgacggtgcc ggcgaactcg tggcccatca cgaggggcgg ggtacggttg cccatcctgc
     2401 ccaggtagcc ttcgatctcc gagccgcaga tccccgttgc ggtggggcgg acgacgacgg
     2461 tcccgggctc gacggccggc tcgggcacct cctcgacgga catctcctca ggcccgtgcc
     2521 acacgatacc cttcatactt ccaccttccc gttgcgagca tctcagataa tctatcttac
     2581 tcctgggccc gccgcgctgc gatcagggcc gcacgagcgc cacggcccta acgggcgagc
     2641 ccgagccgcc tttgaacttt agcggcagcg cgagaaagag gcagaacggc ggcaaacggt
     2701 ccaggttctt caggttctcg acgatgtaca cctcactgcc aagcagcgcg tagtgggccg
     2761 ggtgctccgt agagccggcg gcgtctatcg cgagcgtatc gcacccaacg agcgtgacgc
     2821 ccttaccgac aaggtactgc gcagcctcac cggagaggcc aggccagcct tcgaggaagc
     2881 ggcgtccctc agggcctgtc gcccacctga ggtcccatcc gtagcggatc aaaacccggt
     2941 ctcccgcttc gatgggagca ttctcttcct cccaggcctc gatgtacccg accttcacca
     3001 aatcgtctgc tccgaggtcc gtggccccga tggtcgcagc gcgtcccgcg agacggccga
     3061 gagggatctc gtctgtcccg taatgtgcag gaccctcgga gatgaagtga aggggcgcgt
     3121 ccatgtgggt ccccgtgtgt tcgctcatcg tgaggccgta gtggcgggcg gcgtcaccgt
     3181 attcatagga ctcgtagaga gtcttgccga agcgggcgtg cgtcgggtaa gcagggatgt
     3241 cctcctccag ggtgtgggag aggtcgaccc actcgtagcc ggcgaaaggt gaagtcactc
     3301 cttcgggctg tagcggccgg ggtcctggaa gacgtctatg acgcaggttc ctgcttcgag
     3361 gatggccgtg tgttcggcac cgcccgggat gtcgtaggtc tcgccgggtt cgtaggtggc
     3421 aggctcgcca tctatggtca ggtgaagcag gccgctcacg actatacccc actgcgcgcc
     3481 gtgggagtgg ggagggacct cgaacctctc ggcggccctg aagaagaccg cctggccgtc
     3541 gcggcctgag atcatatgtc cctccaatcc atcgaagggc gtgtctacct gcgggaggtc
     3601 ctggatccaa cgcgcataag ggttcaaagc ggctcctttc cctgcgagcg gcgcgtttgc
     3661 gccgccggtt agggcgtcat tatactgacc gttccaaaag tgtccggggg tgagatggaa
     3721 cgcctgagtg ggaagaggag cattgtcacg ggcgcgggct cgggcatcgg ccgtgccatc
     3781 gccctcaggt tcgccgcgga gggagcgcga gtagtcatct cggacgtgga cgaggaggcg
     3841 gccgcgagcg tggcagcaga agccggaggc gagaccctcg tccgcaagac agacgtcacc
     3901 agcactagcg aggtcgagac gctggtgcag acctcggtcg aagagtgggg cggcctcgac
     3961 gttatggtca acaacgcagg gataggcgta gccggcacca ccacagttac cacggaggag
     4021 gactacgaga gggtgatgga tgtgtgtctc aggggcacgt tcctcgggat gaagcacgcc
     4081 attccagcca tcagggattc ggagggaggg tcggttatca acatgtcttc ggtggcggcg
     4141 ctcgtcggga tagccgacag ggccgtctac agtgcggcga agggcgcgat cctcgccata
     4201 acccgcgccg ccgccatcga ccacgtagaa gaaggcgtga gggtcaactg catagcgcca
     4261 ggcactgtag ataccccctg gatcggccgc atcaccgcgg gctacgacga ccctgaagag
     4321 gcgcgccgga atatgcaagc ccgccagcca catggcaggc tggtcacgcc ggaggagatc
     4381 gccgcgatgg cggcctacct cgcctcagac gaatcggcat cagtgatcgg ggcgtgcatg
     4441 gtcgtggacg ggggcgtgac agccaggtga gtgaggccca caggcgcacg gtcgagtccg
     4501 gctacgacca gatggccgaa gagtatctgg ccaccaagga tcccgaagat ccgctcgcac
     4561 tgggagctct ggaggatctg gccttactct taccgtcgga cgccgccgtg ctagatcttg
     4621 gttgcggcgc tggcgtgccc gtaacacgct ggctcgccga cagagggttc gccgttacag
     4681 gtgtcgacgt ctcggcaaag caactggagt tggcccgtac aaacgtccca gaaggcacct
     4741 tcctcaaagc ggacatgacc gaagtggttt tcgcccctga gtcctttgac gccgttctcg
     4801 ccttccactc catcatccac gtgccccgca ccgaacatcc cacgcttctg gagagcgtcc
     4861 accgctggct cgagccagga ggcgccctcc ttgcgactat gaccgtagcg gactacgagg
     4921 gtcgtgatga ggactgggaa ggatgggggg cgcccatggt gtggagccac tacgacaaga
     4981 acgccaacgt ggcgatgctg cgggacgccg gcttcgagat aaggtacgcc gagccacgca
     5041 caggcggggg cacggccgac gagacggaga cctggctctg ggtccttgcc agcaagaagt
     5101 cgagagaagg tacgacgtga ccaccataac cagggtagta gcccgcgata tccgcttccc
     5161 gacgtcgctg gatctctccg gctccgacgc catgaacgag gcccccgact actcggccgc
     5221 ctacgtcgtg ctcgagacgg acggcggact ggaaggccac ggctttacct ttaccatcgg
     5281 gcgcggcaac gagctgtgcg tcgccgcgat cagagcgctc gccccgctag tcgaggggag
     5341 gaccctcgag tcgttcaccc gcgacatggg cgctttctgg ggtcacgtaa cgggagacag
     5401 ccagctcagg tgggtcgggc ctgagaaggg tgtgattcac ctcgctacgg ccgccatcgt
     5461 caatgccgtc tgggatctct acgcgaaggc cgagcgcaag cccctgtgga agctcctctc
     5521 ggacatgagc cccgaagagc tcatctcttg cgtagacttc cgctacataa ccgacgctat
     5581 cacacgtgag gaagctctcg agatcctacg tcgaaacgag aagacgaagc cggaccgtga
     5641 ggccgagatg ctggaggacg gtttccccgc ctacaccacg tccgccgggt ggctagggta
     5701 tccggacgag aagctgcgct cgctcgtgcg cgaggcggta gagaagggct ggacgcactt
     5761 caagatgaag gttggcgggg acatcgagga cgacgtgcgg cgagccgccc tgatccggga
     5821 tgagatcggg cccgaccgca ccctgatgat ggacgccaac caggtctggg gcgtgggcca
     5881 ggccatcgag aacatggagc gcctgaaagt attcgacccg tggtggatcg aggagcctac
     5941 gagcccagac gacgtccttg gccacgctcg catagccgag gcggtttcac ctgtcggcgt
     6001 tgccacgggc gagcactgcc agaaccgcgt cattttcaag cagctcttgc aggccgatgc
     6061 catctcgttc tgccagatag atgcctgccg tctcggcggc gtcaacgagg tcatcgccgt
     6121 gctcctgatg gctgcgaagt tcggcgtgcc catctgcccg cacgcaggtg gcgtcggact
     6181 gtgcgagtac gtccagcacc tctccatctt cgactacatc gccgtcggag cctcgctcga
     6241 ggaccgaatc ctcgagtacg tggatcacct ccacgagcac ttcgttgacc ctgtggagat
     6301 ccagg
//
LOCUS       DASTKM010000186         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1461149, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000186 DASTKM010000000
VERSION     DASTKM010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461149"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFQ10_07670"
     CDS             <1..745
                     /locus_tag="VFQ10_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET9965256.1"
                     /translation="IIGHFLPGVIHGALAKVVPDKVLAQGADSLWNTQITGEREGGEP
                     FTYVFFSGGGMGARPTGDGLSATAFPSGIRGVPAEVIESISPVVMHRREFRPDSEGPG
                     RNRGGFGQEMELGVRSHSSWVLSAMYDRTRCPAQGIQGGSPGAAGAVSTTDGRGLHPK
                     RQQRMDAEERVVLSLPGGGGYGDPLEREPDLVAHDVENGLVSAGRAREVYNVALTRDR
                     DGNCVVDEEETARLRGSRPDGQASSREQD"
     gene            872..1783
                     /locus_tag="VFQ10_07675"
     CDS             872..1783
                     /locus_tag="VFQ10_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta(1)-pyrroline-2-carboxylate reductase
                     family protein"
                     /protein_id="HET9965257.1"
                     /translation="MEILDAIETEARLPYPELADSIREVMEGRREGKLDAPSRLVLSL
                     PERGNLLVMPAADDELAITKLVTVHPENAEHELPTIQGEVVVMEARTGTRLGILDGAA
                     VTARRTAALSLLAARTLAPHPDGPLLIVGAGTQARSHLEAFREGLGVSEVLLTSRTVE
                     RAVSLAEHARSLGMGARVVDGPEEVLDEVNLVVTATTSQEPILPEEIPDNIFVAAVGA
                     FDPEMAELPPGLVGSSSVVVDTIEGVREEAGDLIQAEHSGAFDWEDATQLEDALLWSN
                     KPGGTIVFKSVGHALWDLAAARTAFGS"
     gene            1780..2787
                     /locus_tag="VFQ10_07680"
     CDS             1780..2787
                     /locus_tag="VFQ10_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HET9965258.1"
                     /translation="MNTQIIVSAVDYHTAGEPFRIISGGVPAILGDTVLDKRLYAMEN
                     LDHIRELVINEPRGHADMYGCFVIEPDDQDADFGVVFFHNAGYSTACGHGTIALAVWA
                     LEGGLVNMAEPETNITIDVPSGRLETVATIEDGRVESVRFRNVPSFVYAEGLTVETSV
                     GPVAADIAFGGAFYACVAAQEVGLGLEAANLADFISLGREIKDSINDEHEILHPLEPR
                     LRDIYGTIFFAEKEGAGSLRQRNVTVFADGEVDRSPCGSGTSARLALLDDSGRLLRGD
                     ALSHESIIGTEFGGRVVGETRVGEYDAVITEVEGSAYLTGYHQFVLRRDDPLGTGFLL
                     R"
     gene            2792..>3174
                     /locus_tag="VFQ10_07685"
     CDS             2792..>3174
                     /locus_tag="VFQ10_07685"
                     /inference="COORDINATES: protein motif:HMM:NF016310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HET9965259.1"
                     /translation="MTETELTPHDEDLLSGHRGEAPRIAMRIILEMARVAGAQRLIDV
                     RSAHIDGCLYHGRAGLDFAERLVSAGGRVVVRATLNVGSLDLLHPSLFRGDEETARLA
                     RKLMDAYVAMGCEPTWTCAPYQLPER"
BASE COUNT          588 a         1001 c         1051 g          534 t
ORIGIN      
        1 catcatcggc cacttcctgc ccggcgtgat ccacggcgcc cttgcaaagg ttgtcccaga
       61 taaagtccta gcccagggcg cagacagcct gtggaacacc cagatcaccg gcgagcgcga
      121 gggcggcgag cccttcacct acgtgttctt ctccggcggc ggcatgggag cgcgtcccac
      181 gggtgacggg ctctcggcga cggccttccc cagtggcatc aggggcgtgc cggcggaggt
      241 catcgagagc atctcccccg tcgtcatgca ccggcgcgag ttccgccccg actccgaggg
      301 gcccggccga aacaggggcg gcttcggcca ggagatggag ctaggggttc gcagccactc
      361 ctcctgggtg ctctccgcca tgtacgatcg cacccgttgc ccggcccagg gcatccaggg
      421 tggatctccc ggggccgcag gggcagtgag caccactgac ggcagagggc tccatcccaa
      481 gcgccagcag cgtatggacg ccgaggagcg ggtcgtcctg agcctaccgg gcggaggagg
      541 ttatggtgat cccctcgagc gcgagccgga cctggtcgcc cacgacgtcg aaaacggcct
      601 cgtctccgct gggcgcgccc gcgaggtcta caacgtcgcc ctgacgcgcg accgtgacgg
      661 gaactgcgtc gtagatgagg aagagaccgc ccgtctgcgc gggtctcgtc ctgatgggca
      721 ggcatcttcc cgagagcaag actgaggagc gaactgtgac gcacgacgtg atcgtggtcg
      781 gagaaatact cgcggacctc gccatcgacg gcacgaccag gtatcctatc ggcctgttct
      841 cccccgagcg gctcgcggct agggagtaga gatggagatc ctcgatgcga tagagaccga
      901 agcccgcctg ccctacccgg agctggcaga ttccatcagg gaagttatgg aaggaaggcg
      961 ggaaggaaag ctggatgctc cctcccggct cgtcctgtcc ctgcctgaaa gaggaaatct
     1021 cctggtgatg ccggcggccg acgatgaatt ggcgattacc aaactggtga cggtccatcc
     1081 cgaaaacgcc gagcacgaac tacccaccat acagggggag gtggtcgtga tggaggcgag
     1141 gacggggaca cgactcggca tcctcgacgg cgccgccgtc accgccaggc gcaccgccgc
     1201 gctctccttg ctcgccgcca ggaccctggc cccacatccc gacggcccgc tcctgatcgt
     1261 gggcgccggg acccaggccc gctcccatct cgaggctttc cgcgaaggcc tgggcgtctc
     1321 ggaggttctg ctgacctccc gcactgtcga acgggccgtc tccttagctg agcacgcgcg
     1381 cagcctgggg atgggggctc gggtcgtaga cggtcccgaa gaggtgctcg atgaggtgaa
     1441 tctggtagtc accgccacga ccagccagga gccgatcttg cccgaagaga tcccggacaa
     1501 catcttcgtc gccgcagtgg gcgctttcga cccagagatg gccgagctac cgccgggatt
     1561 ggtgggaagc tccagcgtgg tggtagatac gatagagggc gtcagggagg aggctgggga
     1621 cctgatccag gccgagcact caggagcatt cgattgggag gacgccactc agctggagga
     1681 tgcccttctc tggtctaaca agccaggagg cacgattgtc ttcaagagtg tcggccacgc
     1741 cctgtgggac cttgccgccg cccgcacggc ttttggatct tgaacacgca gatcatagtc
     1801 agcgcggtgg attaccacac ggcgggcgag ccgttccgca tcataagcgg cggcgtaccc
     1861 gctatcctcg gcgacacagt gctcgacaaa cgcctttatg cgatggagaa cctcgatcac
     1921 atacgagagc ttgtcatcaa cgaacctcgc ggccacgccg acatgtacgg ctgcttcgta
     1981 atcgaacccg acgaccagga cgcggacttt ggcgtcgtgt tcttccacaa cgcgggttac
     2041 agtacagcct gcggtcacgg cacgatagcc ctggccgtct gggcgctgga gggcgggctg
     2101 gtaaacatgg ccgagcccga gaccaacatc accatcgacg ttccctcggg gcggctcgaa
     2161 accgttgcaa ccatcgagga cggacgcgtt gaatcggtcc gcttccggaa cgtgccatcg
     2221 ttcgtgtatg cggagggcct gacggtcgag acgagcgtgg gcccggtagc cgcggacatc
     2281 gcgttcggcg gcgctttcta tgcttgcgtg gccgcgcagg aggtgggact tggtctggaa
     2341 gcggctaacc tagcggattt catcagcctg ggacgcgaga taaaagactc gatcaacgac
     2401 gagcatgaaa tcttgcatcc ccttgagccg cgcctgaggg acatctacgg cacgatattc
     2461 tttgccgaaa aggagggagc ggggtccctg cgacaacgca acgtcacggt ctttgcggac
     2521 ggcgaggtag accgctcgcc ttgcggcagc gggacctccg cgcgtctcgc actgctcgat
     2581 gattcaggaa ggctcctccg gggagatgct ctctcacacg agagcatcat aggcacggag
     2641 ttcgggggac gggtcgtggg cgagacgcgt gtcggcgagt acgacgctgt gatcaccgag
     2701 gtggaaggta gcgcgtatct gaccggctat caccagtttg tgctccgtag agacgaccca
     2761 ctcggcacgg gcttcctgtt gcgttgaagc aatgacggaa accgagctga caccccacga
     2821 cgaagacctc ctctcaggcc atcgcggcga ggcgccccgt atagcgatga ggatcatcct
     2881 ggagatggca cgggttgcgg gagcacagcg gctcatagac gtgcgttccg cccacatcga
     2941 cggttgtctc taccacgggc gggccggcct cgactttgcc gaacgcctcg tctctgccgg
     3001 ggggagggtc gtcgttcgag ccacgctcaa cgtgggatct ctagacttgc tccacccgag
     3061 tctgttccgc ggcgatgagg agacggctcg tctggcgaga aagctcatgg acgcctacgt
     3121 ggcgatgggc tgcgagccga cctggacctg tgcgccctac cagctccccg agcg
//
LOCUS       DASTKM010000187         4194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_933148, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000187 DASTKM010000000
VERSION     DASTKM010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933148"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VFQ10_07690"
     CDS             <1..817
                     /locus_tag="VFQ10_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9965260.1"
                     /translation="MFSGIVTVIFAFVGAEIVTIAASESNEPSRNVARATNAVIYRVL
                     LFYVVSVFFVAAIVPWDTTFAEEVIKSPFTVAFERMGIPFAPDLMNFVVLTAVLSVLN
                     SSLYTTSRMLFALTRHNDAPGFMTNTTRRGVPIWAILTGTSFGYVSVAIYYFFPAEVF
                     TWLINASGAIALFAYLLIAISELVMRRRLEREAPESLQLRMWFYPWLTYLSIAAMLAV
                     LVAMFIIPDQRPLLIASVISLGVILAAYLLRRWFGPPERSPTEIIREHEDASS"
     gene            830..1783
                     /gene="speB"
                     /locus_tag="VFQ10_07695"
     CDS             830..1783
                     /gene="speB"
                     /locus_tag="VFQ10_07695"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HET9965261.1"
                     /translation="MTRYEPADSFETPRFSDVRTFMRLPNTRDLPNADAAIVGAPFDT
                     GATFRAGARFGPEGIRSVSHLLRHYNPSLDVAVFDHISVIDYGDVPVVPGYIESSYER
                     IAKGLEPIHRAGVVPIVLGGDHSIALPELRAAAAVHGPLALVQFDSHPDTWDSYFGER
                     HTHGTPFRRAVEEGLLDTSRSIQVGMRGSIYDRGDWDDAREMGFDLVPTDEVRELGIP
                     SVIDRISGRTEGAKVYVSFDVDFVDPAYAPGTGTPEIGGFTSREAQEFVRGLAGLEIV
                     GCDVVEVYPAYDHAQITALFAANVAHEVLSLIAFSRKTGKA"
     gene            1878..3218
                     /gene="gabT"
                     /locus_tag="VFQ10_07700"
     CDS             1878..3218
                     /gene="gabT"
                     /locus_tag="VFQ10_07700"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HET9965262.1"
                     /translation="MLESTRIKTEIPGPKSRALMERRHNAVSSGVGIATPMFAREAKG
                     ALLTDVDGNTFIDFGGGIGVMNVGHTDSRVVEAVKEQAERLTHSCFYVTEYEGYLQLA
                     EKLNALVPGDTEKRSFFVNSGAEAVENAAKIARYYTGRPAVIAFENSFHGRTMFAMTL
                     TGKTDPYKKGFGPFVPEVYRVPAPYAYRCPAGRDCSGGCRGDCFAAIEKAFVSTVHPS
                     SVAAIIVEPVSGEGGFIPFPDFYMRRLRELCDEHGILMIVDEVQTGFGRTGKMLAVEH
                     SGVEPDIITTAKSMGGGLPIAGVTGKAEIMDSVHVGGLGTTYGGNPIACAAALAVLEA
                     FEEEDLLARANVLGERVMAAMREIQDRHPDFVGDVRGRGPMAAMEFVKDPESRAPDKE
                     RTAKVIGAALQEGLLLLSAGQYGNCIRTLMPFVITDEELEEGLAVLGRAVEAAA"
     gene            complement(3220..3783)
                     /locus_tag="VFQ10_07705"
     CDS             complement(3220..3783)
                     /locus_tag="VFQ10_07705"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9965263.1"
                     /translation="MRTTPMEVKRRRFTVHDYHRMGEAGILHEDDRVELIEGEIVEMT
                     AIGTRHFSCVNGLTRLLVVSVGDEAIISVQNPVWLNEYTEPQPDLAVIRPRDYRESLP
                     EPDDVLLLIEVSDTTLPYDRGVKLPLYARAGIREVWIVDLPGETIERYTAPSANGYRR
                     VEQMRRGQTLESTSLPGLAPSVDEVLA"
     gene            complement(3862..>4194)
                     /locus_tag="VFQ10_07710"
     CDS             complement(3862..>4194)
                     /locus_tag="VFQ10_07710"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET9965264.1"
                     /translation="INAEDPDQDFMPSPGEISWLEVPGGPGVRVDSAIYQGYQIPPFY
                     DSMVGKLIVWALTREEAISRARRALREYRLEGVKTTIPLHLRLLKGEAFRSGEYHTGY
                     LEELLNGD"
BASE COUNT          747 a         1331 c         1333 g          783 t
ORIGIN      
        1 gatgttcagc ggcatcgtca ctgtgatctt cgcgttcgtc ggggcggaga tcgtcaccat
       61 cgcggcctcc gagtccaacg agccgagcag aaacgtggcc cgtgcgacca acgcggtcat
      121 ctacagggtc ctgctcttct acgtggtctc ggtcttcttc gtcgccgcca tagtgccgtg
      181 ggataccacg ttcgccgaag aagtaatcaa gagcccgttc accgtcgcgt tcgagagaat
      241 gggcataccc tttgcgcccg acctgatgaa cttcgtcgtc ctgaccgctg tgctctccgt
      301 ccttaactcc agtctctaca ccacgtcgcg gatgctcttc gcactcacgc ggcacaacga
      361 cgcgccggga tttatgacca acaccacccg ccggggcgtg ccgatctggg cgatcctcac
      421 cgggaccagc ttcgggtacg tgtccgtggc gatctactac ttcttcccgg cggaggtctt
      481 cacgtggctg atcaacgcct cgggagcgat cgcgctcttc gcctacctcc tgatcgccat
      541 ctcggaactc gtcatgcgcc gccgcctgga gcgcgaggcg cccgagtcgc tccagttgag
      601 gatgtggttc tacccatggc tgacgtacct gtcgatagcc gccatgttgg ctgtgctcgt
      661 cgccatgttc atcatccccg accagcgtcc cctgctgata gccagcgtca tcagcctcgg
      721 cgtgatcctg gcagcctacc tgctccgccg ctggttcgga ccgccggaga ggtcccccac
      781 cgagataatc agggagcatg aggacgcaag ctcctgatca gggcgcctct tgaccaggta
      841 cgaacctgct gactccttcg agacgccgcg cttctcggac gtgcgcacct tcatgcgcct
      901 gccgaacacg cgtgacttgc cgaacgcaga cgccgcgata gtaggcgcgc cattcgacac
      961 aggcgccacc ttccgcgccg gagcccgctt cggccctgag gggatacgca gcgtctccca
     1021 cctcctcagg cactacaacc cgagcctgga tgtagcggtc ttcgaccaca tctcggttat
     1081 agactacggc gatgttcccg tcgtcccagg ctatattgaa tcgagctacg agcgtatcgc
     1141 gaagggcctc gagcctatcc accgcgccgg cgtcgtcccg atagtcctcg gcggcgatca
     1201 ctccatagcg ctgcctgagc tcagggctgc cgctgcagtc cacggaccgc tggcccttgt
     1261 gcagttcgat tcgcatccag acacctggga ctcgtatttc ggggagcgcc acactcacgg
     1321 cacgccgttc cggcgggctg tcgaagaggg actcctcgac acctcgcgct ccatccaggt
     1381 cgggatgagg ggttcgatct acgacagggg tgattgggat gacgcgcgag agatgggctt
     1441 cgacctcgtc ccgacagacg aggtccgcga gctcggtatc ccgagcgtga tagaccgtat
     1501 cagcgggcgg accgaaggcg cgaaggtcta cgtttcattc gacgtggact tcgtcgaccc
     1561 cgcgtacgcg cccgggacgg ggacgccgga gataggaggc ttcacaagcc gcgaggcgca
     1621 ggagttcgtg cggggactcg ccggcctcga gatcgtcggc tgcgacgtgg tggaagtcta
     1681 ccccgcctac gaccacgccc agatcacggc cctctttgcc gccaacgtgg cgcacgaggt
     1741 ccttagcctg atcgccttct ccaggaagac gggaaaggcg tgagctacgc gctcgcctat
     1801 ctcactcggg ggtatcgctc gggagctgac ggagagtata ctggaatgaa tcaacccgac
     1861 aggaagaggt ggcgactatg ctcgagtcca cgaggatcaa gaccgagata ccgggtccga
     1921 agagccgggc tttgatggag cggcggcaca atgccgtctc ttcgggggtg gggatcgcca
     1981 caccgatgtt cgccagagaa gcgaaaggag ccctgctaac cgatgtcgac ggcaacacgt
     2041 tcatagactt cggtggcggc atcggggtga tgaacgtagg gcacacggac tcccgggtgg
     2101 tcgaggcggt aaaggagcag gccgagagac tcacccacag ctgcttctac gtcaccgagt
     2161 atgagggcta tctgcagctg gccgagaagc tgaacgcgct ggtaccaggc gacacggaga
     2221 agcgctcgtt cttcgtcaac tcaggcgccg aggcggtcga gaacgcggcc aagatcgccc
     2281 gctactatac ggggcgtcct gcggtgatcg ccttcgagaa ctccttccac gggcgcacga
     2341 tgttcgcgat gaccctcaca ggaaagacgg atccctacaa gaagggcttc gggcctttcg
     2401 tccccgaggt ctacagggtc cctgctccct acgcataccg ttgcccggcg ggcagggact
     2461 gttccggcgg ctgccggggc gactgcttcg cggccatcga gaaggcgttc gtgagcacgg
     2521 tgcacccgag cagcgtcgcg gcgatcatcg tcgagccggt gagcggggag ggcggcttca
     2581 taccgttccc cgacttctac atgaggagat tgcgggagct gtgcgacgag cacggcatct
     2641 tgatgatcgt ggacgaggtt cagacgggat tcggcaggac gggcaagatg ctcgccgtcg
     2701 agcactcagg ggtagagcct gacatcatta ccacggccaa gagcatgggc ggtgggctcc
     2761 ccatagccgg cgttacgggg aaggcggaga tcatggactc tgtccacgtc gggggacttg
     2821 gcaccaccta cggcggcaac ccgatagcct gcgccgccgc cctcgccgtg ctcgaagcct
     2881 tcgaggagga agaccttctc gcgcgtgcca acgtcctcgg cgagcgcgtc atggcggcga
     2941 tgcgcgagat acaggaccgc caccccgact tcgtcggcga tgttcgcggc aggggcccga
     3001 tggccgccat ggagttcgta aaagacccag agagccgcgc ccccgacaaa gagcggaccg
     3061 cgaaggtgat cggggctgcc ctccaggaag ggctcctgct cctcagcgcg ggccagtacg
     3121 gtaactgcat ccgtaccctg atgcccttcg tcatcaccga cgaggaactg gaggagggac
     3181 tggccgtcct cggacgcgcc gtcgaagccg cagcctgaat caggcgagca cttcgtccac
     3241 gctgggagcc aatccgggta gcgatgtaga ctcgagtgtc tgtccgcgac gcatctgctc
     3301 tacgcgccgg tagccgttcg cggaaggggc ggtgtaacgc tcgatggtct cgccagggag
     3361 gtccacgatc cagacctccc tgatcccagc ccgcgcgtag agcggtagct ttaccccgcg
     3421 gtcgtagggg agggtggtat ccgaaacctc gatcaggagc agcacgtcat cgggttcggg
     3481 gagtgattcc ctgtagtcgc gagggcggat gaccgccagg tcgggctggg gctcggtgta
     3541 ctcgttcagc catacaggat tctgaacgct gatgatggcc tcgtcgccga cgctcaccac
     3601 cagcagccgg gttagtccgt tgacgcacga gaaatgtctg gtgcctatgg ctgtcatctc
     3661 gactatctcg ccctcgatga gctccaccct gtcgtcttcg tgcaggatgc cggcctcgcc
     3721 catgcggtgg tagtcgtgga cggtgaagcg ccgccgcttg acctccatcg gtgttgtccg
     3781 cacgcaaacc tccggtacct cgctcgtctc atgctggagt atactcggag cgcgaagaga
     3841 agcctttagg ttttcctcct ttcagtctcc gttcaacagc tcttccaggt agcctgtgtg
     3901 gtactcgccg gatcggaagg cttctccttt caggagcctg aggtggaggg ggatcgtcgt
     3961 ctttacgccc tcgaggcggt attcgcggag ggcgcgcctg gccctgctga tggcttcctc
     4021 tctggtgagg gcccagacta tgagcttgcc caccatcgag tcgtagaacg gggggatctg
     4081 gtacccctgg tagatggccg agtctaccct gacgcctggg ccgcctggaa cttcgagcca
     4141 ggagatctcg cctggagacg gcataaagtc ctggtcgggg tcctcggcat tgat
//
LOCUS       DASTKM010000188        15275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_404270, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000188 DASTKM010000000
VERSION     DASTKM010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15275
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404270"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VFQ10_07715"
     CDS             <1..970
                     /locus_tag="VFQ10_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965265.1"
                     /translation="YSIDELARQIRFADELRERYNVLIQTAMQTDVPGATVGLLSSLV
                     DADIRYLSVAHNYAGRSAPHLVGGQELTRPFYWRAPSGKRLLVWYTDSPHGSAYMEGN
                     LVGLGEDYQATVEALPGYLAALAESPYPYRGRGESILGWTGLDPSSEVTKVPYPYDVL
                     HLRVQGAFSDNASPSIVPAGIVREWNERWAYPQLRLATNSAFFTETEERLAGSIETYS
                     GDWTDWWVDGIGSGARHLGFNRRAQADVRTAQTLHTLADTLTDDGADPWNEIDEAYES
                     MALFDEHTWGARIPGWTAWRGGNQGRFNGRERRVSRGTLMRNPTPS"
     gene            874..2445
                     /locus_tag="VFQ10_07720"
     CDS             874..2445
                     /locus_tag="VFQ10_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HET9965266.1"
                     /translation="MDGLERRESGALQWERKAGFARDAYEKSNALVMSGVHRLSYAFG
                     RSGDSLASVSVFNPSRWERTDPVSVFVPESRTELQRPLAVVDSATRERVPHAVEDQKN
                     AQFRVRGSRLSFVASDVPACGYRRYDLVEGDDEYEDPESGDEPSVENEYYRVRFDLAR
                     GRVTELLDKTSGLNLVDADAPFGFNQYVYDRYASAPHFNHLSSRIQATDLTLLCGRSA
                     GGLAVVTQRSSNPVWDRVTVRLVDERADIIEYTLTLFRGVRRLHITNRIHKVGTPEKE
                     SVYFAFPFDVRDPSIHYEITGGIDSPEAPHVPGSADHMRAIRHWVGLENERVRLAWAT
                     MEAPLVQFGNIHLPYFPFPETIGPEIANPATVYSWALNNIWDTNFPSQQQGEMEFSYA
                     VASGEDMETPELGMRTAAALTTPLLGILSAPSAENDLPERGSFCSVEHPLVDVVALMS
                     SRRGHDLTVMLQSLSSENEEVCVSFGLLGVARAWVGSHLEKNLEEARVDGANVRFVVP
                     AGSLVSLAVDLERGR"
     gene            2670..3506
                     /locus_tag="VFQ10_07725"
     CDS             2670..3506
                     /locus_tag="VFQ10_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HET9965267.1"
                     /translation="MSTAPDPLATFLDYYPGGWQEIFPNGGAPSSYLGARFGQHGEVS
                     NLPWDYHIVEDGEQSVAVRFSVRTQKTPFLLQKTLRLRSGDSGIRFEETLTNESAVHM
                     RAMWGHHIVFGRPFLEEGCRVRVPEGATVVPHEGPIDPDGRRVRGGDNYSWPVVAGES
                     GAEVDLSVLPAWGTSSEIVYIKDLPEGWYEIDNPKRDLGFRVEWDVRQMPYLWFWQEF
                     GASRFYPWYGRNYNVGLEPFSSFPTNGIEEAVENDSALSVGPSETLRFALRAEVLEEG
                     GV"
     gene            3503..4300
                     /locus_tag="VFQ10_07730"
     CDS             3503..4300
                     /locus_tag="VFQ10_07730"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9965268.1"
                     /translation="MTDEFRGKVAIVTGGGAGIGLAAAGRLSREGASVVICSDREDQA
                     RQAAGGLREEGLEVRGMRGDVTSSTDMEGLVDFAVEVYGGVDVLVNSAGVQRYGTVTE
                     TEEDVWDEVLDVNLKGIYLAARHTIPEMRRRGGGAIVNVSSVQAIASQTGVAAYTASK
                     GGINALTRAMALDHAEENIRVNAVCPGSVDTPMLRWAADLFKGENSVEATVEDWGRMH
                     PLGRVARPEEVAEVICFLAGPKASFVTGADYRVDGGLLAALGVRLPE"
     gene            4325..5578
                     /locus_tag="VFQ10_07735"
     CDS             4325..5578
                     /locus_tag="VFQ10_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET9965269.1"
                     /translation="MKIADIRATTVTVPLEAPLQHASGAHWGRFVRTIVEVETDDGLV
                     GLGEMGGGGESAEQAFEGLKSYLIGHDPFELEALRFKIMNPTASLYNNRAQLHAAIEF
                     GCLDLVGQKLGVPVHTLIGGKLRDRVPFASYLFFRYANAEAGEPEIRSADQLVEHARR
                     LKEEHGFTSHKLKGGVYPPEYELGCYRALASAFPEDTLRFDPNAAFSVEEGIRFGLAI
                     EDLKNDYYEDPTWGLNGMRRLREKVKIPLSTNTVVIDFEQLATNVLNPAADVILLDTT
                     FWGGIRPCVKAAGVCETFQLGVAVHSSGELGIQLATMLHLGAVLPNLSFSADAHYHHL
                     QDDIIDGGMFEYEGGSIKVPDGPGLDVSLDRDKLDEYAELYKELGGYPCDRDPGYPGR
                     YPLVPKQRFADPNKPMTPQIPTGQR"
     gene            5588..6445
                     /locus_tag="VFQ10_07740"
     CDS             5588..6445
                     /locus_tag="VFQ10_07740"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET9965270.1"
                     /translation="MEPILPLESAEVFFDGIFSEPRLRHPEGIAIDADGDIWCGGETG
                     EIYRISADGSGIETVASTDGFILGLAFDDDQNLYACDMRHSVVFRLDTRTGRLVHFAN
                     GGGGNEMRIPNFPVIDGSRNCLYVSDSYAPTEKGPGVWRFDLESGEGGLWYDRAMTFA
                     NGMALSPDKDYLYVAESFAGRVIRIPIDENGSPEVGELFVEGLERVPDGLAFDAAGNL
                     YIACYEPSRMYRVSPEREIHLFIDDPIAHTMCHPTNCAFRGEELFTSNLRRWHITRIE
                     VRSEGLALP"
     gene            complement(6545..9823)
                     /locus_tag="VFQ10_07745"
     CDS             complement(6545..9823)
                     /locus_tag="VFQ10_07745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET9965271.1"
                     /translation="MNGESPDDFVHLHVRSGFSFGFGVARPEELVEAAARMRMSSLAL
                     TDRDGLYGIPRFLEAADEAGILPIVGTQVSTAGGHLVLLAEAIEGYRSLCRLITDYRT
                     SSEDRRRPACPLGKILEHEEGLICLTGAVPFGLIPSLILAGQRKEATEVLHRLHEAFG
                     DRLYVELTDDETATGRRSVGKVAAFARAAGVPVVAAGDVAYLFPSDHRLHDVLVAAAN
                     LTALPGPEYRPTDRLHLRSGKEIRRLFEGYPDALANVADVARRCAGAVKLSGRVHMPA
                     AILPSGKTAGKMLLELTLAGARRRYGRRAGDGPTAVELKSRLGRELRCIHALGFAPYF
                     LIAHEAKEIAKSKGIPVTGRGSAANSLVSYCLGLSEPEPFSNRLRFERFLHEGRKDPP
                     DVDLDFCSVRRDEVRFEMIHRYERRGVAEAATVQTMSLRGAVRVAARSLGHSPQEIDT
                     LSRQVPTRFHDRDRIYAGLSGWEESLAEPAMQGHPLQDTERYRLLLELSAGLAGRIKE
                     GGTHSGGMVFGTAEHHLSELVPLEPSGTEGLLRCQYDKDDLEHVGLPKLDLLGLRMHT
                     ALHKAGELASRRLGRRVDPYDLPPDDKKTYALIRTGRNAGMFQLESPGQMHLSQRLGP
                     RRLADLVAQISLFRPGPVRGDLVTPYVKRRNGRESYEVILPELEEILRPTYGVLIFQE
                     QALAVANAVAGFTLAEGDLLRRAMTKDRGPGAMQELRLEFMRRARENGVPAAKADEVF
                     SWMEGFSVYGFSAAHAASFAELAYASAYMRRHYPAEFFAAVLDSQPMGFYSPRLLLNE
                     ARRIGLSVLPPDVHLSEAGFAVEEDGSALRVGLRYCKGLSERAISSIVAERRKKPFVS
                     VADLYGRTAVEKDALENLIRGGFLDSLAGGSERSRLLGESAALPKKRRRGRQEEIPIE
                     HPASWWAMRERRSVGHLPLTDTARERMEWEVLGLNVHRHPLVPYRDALEELGVVPSVG
                     IRELPHGTSARAAGLIECLQSPPTKSGYRVYFLLIEDEWGLLQATIFRSVYERCGDIL
                     HHEGAFLVEGRVEQTVDKGFAFLVHCIESLRNILFEARVPVPRVASSPGAFLRARRRS
                     RKAG"
     gene            complement(9820..10245)
                     /locus_tag="VFQ10_07750"
     CDS             complement(9820..10245)
                     /locus_tag="VFQ10_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965272.1"
                     /translation="MEASVSEIHSYPLRPEGFEEPLFPTRESRLAVLDHLLGAPWLSR
                     ASEMEERGGQAAHARWEPVELENCHGRLRIVWASGRRRCRRRRHVVEVLGRWREVRAW
                     WDETRAKDRSLARLLLSDGSVVDLARENGGEWFLVGVTD"
     gene            10503..11135
                     /gene="lexA"
                     /locus_tag="VFQ10_07755"
     CDS             10503..11135
                     /gene="lexA"
                     /locus_tag="VFQ10_07755"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET9965273.1"
                     /translation="MVPENWKIRMEMLRRLARANVRGEGPPSVREVGEAVGLRSSQTA
                     YKHLKKLEEAGYIEREGGRARGIRLTEKGWEAAGEMPLLGRIAAGRGLEAVTVGDEAY
                     SLAAELVGSRLGRRRYLLRVVGQSMIGAHIADGDLLVVEEDEDPPDGAVVAALIGGGE
                     EVTVKRLYREGDLLRLRPENGDHEDLVVPAEDVSIQGRVVYVVHPPRGRI"
     gene            11329..12462
                     /locus_tag="VFQ10_07760"
     CDS             11329..12462
                     /locus_tag="VFQ10_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9965274.1"
                     /translation="MTRPVVLLILVAFAAVFGFQLLLSVVPLYANEAGGGSSGAGLAT
                     AAFMLSTVLTQIQMPRILARYGYRRVLAAGLLFLGVPALFYAYAQTLVPILAVTLLRG
                     VGFGIVTVVFAAMVVELAPAGRRGEALGLLGVAFALPNIFCNALGLWLVGQFGYEVVF
                     LLGGVAPLLGLGMVIGIRSAAPSGRGEEEEGAGFFAGLGRSPLLRIFLLFAATTMAAG
                     VVLTFLPLAVPGSGVFSAASALLVVGITSTASRWWAGRFGDRRDPRLLLAPGLLACAA
                     GMVCLPLGGIVLLIGAVLFGTGFGLLQNATLILMMGRVSKSEYGLGSTLWNAAFDTGT
                     GIGAFSFGLVISAVGFSWSFFICSALVASALLLVILDYSSTQD"
     gene            complement(12506..12778)
                     /locus_tag="VFQ10_07765"
     CDS             complement(12506..12778)
                     /locus_tag="VFQ10_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9965275.1"
                     /translation="MRHQGYVMEMPQSGEHFRGRENMRAFQRARSDHSTPPRSLQLRR
                     VLVKEGLWVVEAVIDYGGGQVFDVVLISELRDGKMWRAQWVERMEP"
     gene            12900..13385
                     /locus_tag="VFQ10_07770"
     CDS             12900..13385
                     /locus_tag="VFQ10_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965276.1"
                     /translation="MYGRGMMGYTGGRRTMVPYGRPFPPRFMMGLVIGGLFLLFVAGP
                     AFHFLPFLFLLIWAVPFFLAPAFVAVARGLSGLSESLTRKGGTVTEESKERELLEALA
                     RRGELSPALAALETSLTVAEANRMLSDLANSGHLEVRAQEGRLGYALWERDRRELTDG
                     A"
     gene            13413..13973
                     /locus_tag="VFQ10_07775"
     CDS             13413..13973
                     /locus_tag="VFQ10_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET9965277.1"
                     /translation="MFGPKAMKGFFGGWHRPGFGHGPRRGRMFEKGDLKYVILDLLKD
                     EPSHGYEVIRKLEERSRGFYSPSPGSVYPTLQLLEDMEYVSATQRDGKKVYAITDEGR
                     KFLDENQRSVNDIWGKFGGSWDPGVASELHEIRHDLMDLGKLFGQKMREGRVDQEKLA
                     RIRAVISAATREIEDILRDRDEVQRA"
     gene            14077..14802
                     /gene="pcaH"
                     /locus_tag="VFQ10_07780"
     CDS             14077..14802
                     /gene="pcaH"
                     /locus_tag="VFQ10_07780"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HET9965278.1"
                     /translation="MAQRKGEEAAGYRREDDGVQPPYLYPDYVATRLRAPKKPLIILP
                     RTLSDTTGPAYGRGAVGDLDDDLTRQHSGEPLGERIIVTGRVLDGDGRPVKSSLVEIW
                     QANAAGRYTHHLDQHPAPLDPNFSGAGRCLTDAEGRYRFVTVKPGAYPWKNHDNAWRP
                     AHIHFSLFGPAFRSRLITQMYFPGDPLLEHDPICQSVRDPKSRQRMISTFDLETTQPE
                     WALGYRFDIVLEGRDATPMENHR"
     gene            14799..>15275
                     /gene="pcaG"
                     /locus_tag="VFQ10_07785"
     CDS             14799..>15275
                     /gene="pcaG"
                     /locus_tag="VFQ10_07785"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HET9965279.1"
                     /translation="MSSVPTPSQTIGPFFHDALLDKNYSELVSPDHPEGIRIKGTVYD
                     GAGGVVPDAMVEIWQADTAGRYDHPTDDREDISLHEDFSGFGRSGTDAGGEFSFLTVK
                     PGPVPGPDGKVQAPHVMVSVFARGLLKRLVTRIYFPDEEVANAVDPVLSSIEDPELR"
BASE COUNT         2858 a         4715 c         4886 g         2816 t
ORIGIN      
        1 gtattcgatc gacgaactgg cgcgccagat acgcttcgcc gacgaactac gcgagcgcta
       61 caacgtgcta atacagaccg cgatgcagac ggacgtgcca ggggcgacgg tcggtcttct
      121 tagttccctg gtggacgcgg atatccgcta cctttctgta gctcacaatt atgcgggtcg
      181 ttctgccccg catcttgtgg gcggtcagga gcttacacgc cccttctact ggcgcgcacc
      241 cagcggcaaa cgcctgttgg tgtggtatac ggactcgccg cacggtagcg catacatgga
      301 gggcaacctg gtcggtctgg gggaggatta ccaggccacg gtcgaagcgt tgccgggcta
      361 tctggcggcc ctcgcggaga gtccttaccc gtaccgggga cgcggggaga gcatcctcgg
      421 atggacgggc ctggatcctt cctccgaggt gacgaaagtg ccgtatcctt acgatgtgct
      481 ccacctgagg gtccagggcg ctttctctga caacgcctct ccgagcatag tacctgccgg
      541 gatcgtgcgc gaatggaacg agaggtgggc ttatccgcaa ctacgcctgg cgaccaacag
      601 cgcgttcttc accgagaccg aagagaggct tgcaggaagt atcgagacct actccggcga
      661 ctggaccgac tggtgggtcg acggcatagg atccggggcg cgccacctcg gcttcaaccg
      721 gcgtgcccag gcggatgtaa ggacggccca gaccctgcac accctggccg atacgctcac
      781 cgatgacggc gccgacccgt ggaatgagat tgacgaggca tacgagagca tggcgctctt
      841 cgacgagcat acctgggggg cgcgaatccc tggatggacg gcctggagag gagggaatca
      901 ggggcgcttc aatgggagag aaaggcgggt ttcgcgaggg acgcttatga gaaatccaac
      961 gccctcgtaa tgtcaggtgt ccaccgcctc tcttacgcat tcgggcggtc cggcgactca
     1021 ctcgcctccg ttagcgtatt caacccaagc cgctgggagc gcaccgaccc ggtcagcgtg
     1081 ttcgtgcccg agagccgcac ggaactccag cgccctctcg ctgtggtcga tagtgccaca
     1141 cgagagcgcg tgcctcacgc agtcgaggat cagaaaaacg cccagttcag ggtgcgcgga
     1201 agccgcctca gcttcgttgc cagtgatgtt ccggcctgcg gctacaggcg ctacgacctt
     1261 gtcgaagggg acgatgagta cgaagatccg gagtcgggcg acgagcctag cgtagagaac
     1321 gagtactatc gggtgcgctt cgacctcgct agaggtcggg tcaccgaatt actcgacaag
     1381 acttcaggcc tgaacctggt cgacgcagac gccccgttcg ggttcaacca gtacgtgtac
     1441 gaccgttacg ccagcgcccc gcacttcaac cacctctcca gccgcatcca ggcgaccgac
     1501 ctgactctac tttgcgggcg ctcggcaggg gggctcgccg tggttacaca gcggtcctcc
     1561 aaccctgtgt gggaccgcgt aaccgtgcgt ctggtcgatg agagggctga tatcatcgag
     1621 tatacgctga ccctgttccg cggcgtgcgc agactccaca tcaccaaccg catacacaag
     1681 gtcggtactc ccgagaagga gagcgtgtac ttcgccttcc ccttcgatgt aagggacccg
     1741 tcaatccact acgagataac aggggggata gattcacccg aagcgccgca cgttccaggc
     1801 tcggccgacc acatgcgggc gatccggcac tgggtcggcc tcgagaacga gagggtcagg
     1861 ctggcgtggg ccactatgga ggctccgctc gtccagttcg gaaacatcca cctcccttac
     1921 ttccctttcc ccgagaccat cggccccgag atcgccaacc ccgcgacggt atactcctgg
     1981 gcgctcaata acatttggga caccaacttc ccttcgcagc agcagggcga gatggagttc
     2041 tcttacgcgg tcgcctcggg agaagatatg gagacgcccg agcttggtat gaggacagcg
     2101 gcggccctga cgacgcccct gctggggatc ctctctgcgc cgtctgccga gaacgacctt
     2161 cccgagcggg gcagcttctg ctccgtagaa catcccttgg tagatgtcgt ggccctaatg
     2221 tcttccagac gggggcacga cctcacggtg atgctccagt ctctgtcctc cgagaatgag
     2281 gaagtctgcg tctcgttcgg gctgctgggt gtcgcccgag cctgggtcgg gtcgcatctc
     2341 gaaaagaacc tggaagaggc gagagtcgat ggcgcgaatg tccggttcgt cgttccggca
     2401 gggagcctcg tctccctcgc cgttgatctg gaacgtggga ggtaggacag actcgtggga
     2461 gacgaaaagc atcgaaagca gaggaactgg ggcacccgta tcctggagta cgagcacaag
     2521 gggatgcagg tggtcttcct cgaaaacgag aagctgaggg taggggtgct cgtggacaag
     2581 ggtacggatg tcatcgagtt caaccacaag ccgaccgaca ccgacttcgt atggctcgcc
     2641 ccgggaggcg tgaggaatcc acctcgtact tgtcgacggc gccggatccg ctcgcgacgt
     2701 tcctggatta ctaccctggt ggctggcagg agatcttccc caacggtgga gccccttctt
     2761 catatctcgg cgcgagattc ggccagcatg gcgaggtcag caacctgccc tgggattacc
     2821 acatagtcga ggacggcgag caatccgtgg ccgtcaggtt ctccgtcagg acccagaaga
     2881 ctccctttct gctccagaag acgctgcgcc tgcgctcggg agatagcggt atacggttcg
     2941 aggagacgct cacgaacgag agcgcggtcc acatgcgcgc catgtgggga caccacatag
     3001 tcttcggccg gccctttcta gaagaaggct gccgggtgcg cgtgcccgag ggggcgaccg
     3061 tggtaccgca cgaagggccg atagatcctg atggaaggag ggtcaggggt ggcgacaact
     3121 actcgtggcc cgtggtggca ggcgaatccg gagcagaggt cgacctcagc gtactgccgg
     3181 cgtgggggac atcgagcgag atagtctaca taaaggacct tcccgaaggt tggtacgaga
     3241 tagataatcc gaaaagggac ctgggattta gggtggaatg ggacgtacgg cagatgccat
     3301 acctctggtt ctggcaggag ttcggggcct cccgcttcta tccctggtac ggcagaaact
     3361 acaacgtggg cctcgagcca ttctcgagtt tcccgacgaa cggcatagaa gaggccgtcg
     3421 agaacgactc ggcgctctct gtcggcccgt cggagactct caggttcgcg ctcagggccg
     3481 aagtactgga ggagggtggc gtatgacgga cgagtttcgc ggtaaggtgg cgatcgtaac
     3541 aggtggtggg gccggcattg ggctcgcggc cgcagggagg ctgtccaggg agggagcctc
     3601 tgtagtcatc tgcagcgacc gtgaggatca agcccgccag gcggctggcg ggctgcgcga
     3661 agagggcctc gaagtcaggg ggatgagggg tgacgtgacc tcctctacgg atatggaagg
     3721 gctcgtcgat ttcgccgtcg aggtctatgg aggggtagac gtgctcgtca acagcgccgg
     3781 ggtccagcgc tacggaacgg tcacggagac cgaggaggat gtctgggacg aagtcctcga
     3841 tgtcaacctc aaggggatat acctggcggc caggcatact attcccgaga tgagacggcg
     3901 cgggggaggt gcgatcgtca acgtttcgag cgtgcaggcc atagcctcgc agacaggggt
     3961 ggccgcctac acggcgagca agggcggcat caacgcccta acacgggcga tggccctgga
     4021 tcacgccgaa gagaacataa gggtcaatgc tgtatgtcct ggttctgttg acacaccgat
     4081 gctgcgatgg gcggcggatc tcttcaaggg ggagaactcc gtcgaggcga cggtcgagga
     4141 ctggggcagg atgcatcccc tgggaagggt tgccaggcca gaagaggtcg ccgaggtgat
     4201 ctgcttcctc gcaggcccga aggcgagctt tgtgacggga gcagattaca gggtcgacgg
     4261 cggtttgctc gcagccctcg gggtacgatt gccggaatag tcccgcggtc gcataggagg
     4321 aaccttgaag atagccgaca taagagcgac caccgtaacg gtcccattgg aggcgccgct
     4381 ccagcatgcc agcggggccc actggggacg cttcgtgcgc actatagtcg aggtggagac
     4441 cgacgatggc ctcgtcggtc ttggcgagat ggggggagga ggggagagcg cggagcaggc
     4501 cttcgaaggt ctcaagtcct acctcatcgg ccatgacccc ttcgagctcg aggcgctcag
     4561 gttcaagatc atgaacccta cagccagcct ctacaacaac cgcgcccaac ttcatgccgc
     4621 catagagttt gggtgcctgg atcttgtggg gcaaaagctc ggagtccctg ttcacacgtt
     4681 gatcggcggc aagctcaggg acagggtgcc cttcgcgagc tacctcttct tccggtacgc
     4741 gaatgccgag gcgggggagc ctgagatccg gagcgccgac caactggtgg agcatgccag
     4801 gaggctcaag gaggagcacg gcttcacctc gcacaagctc aagggtggcg tctatccgcc
     4861 agaatacgaa ctcgggtgct accgggccct cgccagcgcg ttccccgaag ataccctgag
     4921 gttcgatccc aacgccgcct tcagcgtcga ggagggcatc cgcttcggcc tggccataga
     4981 ggatctgaaa aacgactact acgaggaccc gacctggggg ctcaacggta tgcggcgcct
     5041 gcgcgagaag gtgaagatac cgctctcgac caacaccgtg gtcatagatt tcgagcaact
     5101 ggcgaccaac gtactgaacc cggcggccga cgtcatcctg ctcgacacca cattctgggg
     5161 tgggatccgg ccctgcgtca aagccgctgg ggtgtgcgag actttccagc tcggggtggc
     5221 cgtacattca tcgggggaac tggggataca gcttgctacc atgctgcacc tcggggccgt
     5281 gctccccaac ctctccttct cggccgatgc gcactaccac catctacaag acgacatcat
     5341 cgacgggggc atgttcgagt acgagggtgg ctccatcaag gtccccgatg gccctgggct
     5401 ggacgtcagc ctggaccggg acaagctgga cgaatacgca gagctctaca aggagcttgg
     5461 aggatatcct tgcgacaggg accccggata cccgggccgt taccctctgg tgcccaagca
     5521 gcgctttgct gatccaaaca agcccatgac gccgcagatc cccaccggac agaggtgaga
     5581 taagaccttg gaacccatat tgcctctgga gagcgccgag gtcttcttcg acggcatatt
     5641 cagcgagccg cgactccgtc accccgaggg gatcgcgatc gatgctgacg gcgacatctg
     5701 gtgcggggga gagacggggg agatctacag gatctccgcc gatgggtccg gcatcgagac
     5761 ggtagcctcg acagacggat tcattctcgg cctggccttc gacgacgacc aaaacctcta
     5821 cgcctgcgat atgcgccact ccgttgtgtt tcgcctcgac acgcgaacag gacgtctcgt
     5881 ccacttcgcg aacggcggag gagggaacga gatgcgcatt cccaacttcc ccgtgataga
     5941 cggctccagg aactgtctgt acgtctccga cagttacgct cccactgaga agggaccggg
     6001 agtctggcga ttcgacctgg agagcggaga aggaggactc tggtacgaca gggccatgac
     6061 cttcgcaaac ggaatggccc tctctcccga caaggattac ctgtacgttg cggaatcatt
     6121 tgccggcagg gtaatccgca tccccataga cgaaaacgga agcccggaag taggcgaact
     6181 gttcgtggaa gggctcgagc gtgtgcctga cggtctggca ttcgacgccg cagggaacct
     6241 gtacattgcc tgctacgaac cctcgcgtat gtacagagtt agtccggaaa gagagataca
     6301 tctctttata gacgacccca tagcacacac catgtgccat ccaacgaact gtgcgtttcg
     6361 cggcgaagaa ctcttcacat ccaacctgcg ccggtggcac ataacgcgga tcgaagtccg
     6421 ctcagaaggc ctcgcactgc catgaaagca gtttccggaa gatcttccgg aaactgcgca
     6481 tttcagcacg ctcaggcttc gccttcgctt gtcagcaatt cagcttgtca gctttgtaaa
     6541 agtcttaccc agctttccga cttcttctcc tggcgcgcaa gaaagctcct ggagaagacg
     6601 ccaccctggg cacaggcacc ctggcctcga atagaatgtt tcgcaagctc tctatacagt
     6661 ggacgaggaa agcgaatcct ttgtcgacgg tttgctctac tctgccttct acgaggaacg
     6721 ctccttcgtg gtgcaggatg tcgccgcacc tctcgtagac agagcggaag atcgttgctt
     6781 gcagcaggcc ccactcgtct tcgatcagca ggaagtacac acggtatccg ctcttggtcg
     6841 gggggctctg gaggcattcg atcaggccgg ccgcgcgggc gctggtgccg tgcggcaact
     6901 cccttatccc cacgctcgga accaccccga gctcttcgag ggcgtcgcgg taaggcacga
     6961 gcgggtgtcg atggacattg aggccgagca cctcccactc catcctctct ctcgccgtgt
     7021 ccgtgagagg gagatgcccg acgctccttc tctcccgcat cgcccaccag ctcgcagggt
     7081 gttcgatcgg gatctcctct tgcctcccac gcctacgctt cttcggaaga gccgcactct
     7141 cgccgagcag ccgggatctc tccgaccctc cagccagaga gtcgaggaag cctcccctga
     7201 tgaggttctc caacgcgtct ttttcgacgg ccgtcctccc gtaaaggtcg gcgacggaga
     7261 cgaacggctt tttgcgccgc tcggctacga tggaggagat ggccctctcg gagagcccct
     7321 tgcagtacct gaggccaacc cggagagcag agccgtcttc ttctacggcg aagcctgctt
     7381 cggagaggtg gacgtcgggc gggagcacgc tgagcccgat cctacgcgcc tcgttcagga
     7441 gcagcctcgg gctgtagaaa cccatcggct gggagtcgag gacggcagcg aagaactcag
     7501 ccgggtagtg gcgcctcatg tacgcgctcg cgtaggcaag ctccgcgaag ctggcggcgt
     7561 gcgcggcgga gaaaccgtag accgagaacc cctccatcca cgagaagacc tcgtccgctt
     7621 tggcggcggg gaccccattc tcccttgccc gtcgcatgaa ctccaggcgc aactcctgca
     7681 tcgcgccggg tcccctgtcc ttggtcatgg cgcgtcgcag gaggtcaccc tccgccaggg
     7741 tgaagccagc cacggcgttc gccacagcca gggcctgttc ctggaagatg agaacgccgt
     7801 aggtgggacg tagtatctct tcgagctcgg gtaggatcac ctcgtacgac tccctgccgt
     7861 tccggcgctt cacgtagggg gtgacgaggt ctcctctcac gggacctggc ctgaagagtg
     7921 agatctgggc caccaggtca gcgagcctcc tgggcccgag acgttggctc aggtgcatct
     7981 gtcctggcga ttccaactgg aacatcccgg cgttgcgccc ggtccttatg agggcgtagg
     8041 tcttcttgtc gtctggcggc agatcgtagg ggtcgaccct gcgccccagt cgcctcgaag
     8101 ccaactctcc ggccttgtgc agcgccgtgt gcatcctcag gccgagcagg tccagcttcg
     8161 gcaggcctac gtgctccagg tcgtccttgt cgtactggca gcgcaggagc ccctccgtcc
     8221 cggaaggctc cagcggcacg agctccgaaa gatggtgttc cgccgtcccg aacaccatac
     8281 ccccgctatg ggtgcctccc tccttgatgc ggccggcgag tccggccgag agttccagga
     8341 ggagcctgta tctctccgtg tcctggagag gatggccctg catggccggc tcggcgagcg
     8401 actcttccca gccggagagg ccggcgtaga tcctgtcgcg gtcatggaaa cgggtaggga
     8461 cctggcggct gagggtgtct atctcctgcg gagagtgccc gagcgatcgc gccgccaccc
     8521 tcacggctcc ccgcagggac atcgtctgga ccgtcgcagc ctcagcgacg cctcgacgct
     8581 cgtagcgatg gatcatctcg aagcgcactt cgtcacgcct cacagaacag aagtccaggt
     8641 cgacatcggg cgggtccttg cgtccctcgt gcagaaaccg ctcgaagcgc agccggttcg
     8701 agaacggctc gggctcgctc agccccaggc agtaggagac caggctgttc gcggcgctac
     8761 ctctgcccgt gacggggatg cccttcgact tcgcgatctc tttggcctcg tgcgcgatca
     8821 ggaagtacgg cgcgaacccc agagcatgta tacagcgcag ctctcgcccg agtcgcgact
     8881 tcaactccac cgccgtaggc ccatcccccg ctcttctccc gtagcgtctt ctggcgcccg
     8941 cgagtgtcag ctccagtaac atcttcccag ccgtcttgcc cgaaggcaag atcgccgccg
     9001 gcatgtggac tctgcccgac agcttcaccg cccccgcgca cctccgtgcg acatcagcaa
     9061 cgttcgccag ggcgtcggga tagccctcga agagccgccg tatctccttc ccggatctga
     9121 gatgcaaccg gtcggtaggc ctatactccg ggccggggag cgccgtgagg ttggcagcag
     9181 ccacgagtac gtcgtgcagc ctgtggtcag aggggaagag gtaagctaca tcgccagcgg
     9241 caacgaccgg tacgccagcg gcccgcgcaa aggccgccac ttttcccacg cttcgccgcc
     9301 cggtcgccgt ctcgtcgtcg gtcagctcga cgtagagccg gtccccgaat gcctcatgca
     9361 gacggtgcag aacctcggtc gcttctttcc tttgcccggc aaggatcagg ctggggatca
     9421 gcccgaacgg gacggctccg gtcaggcaga tcaaaccttc ttcatgctcc aggatcttcc
     9481 ccagcgggca agcgggacgg cgccggtcct cagatgatgt cctgtaatcc gtgatgagcc
     9541 ggcacaggga gcggtagcct tctatggcct cggccagcag cacgaggtgc ccacccgccg
     9601 tcgagacctg ggtcccgacg atgggtagga tgcccgcctc atcggcggcc tcgaggaagc
     9661 gggggatgcc gtagagcccg tcgcggtccg tgagcgccag agaggacatc ctcatcctcg
     9721 ccgcagcctc cacaagctct tctggcctcg cgactccgaa accgaaggag aacccgctcc
     9781 tgacgtgcag gtggacgaag tcgtccgggg actctccgtt caatccgtca cccctacgag
     9841 aaaccactcc ccgccgttct cccgcgccag gtcgacgacc gagccgtcgg agaggaggag
     9901 cctcgcaagg ctcctgtcct tcgcccgcgt ctcgtcccac cacgcccgga cctcccgcca
     9961 acgtccaagc acctcgacga cgtggcgcct cctgcggcac cgtctccggc ccgaagccca
    10021 cacgatccgc aggcgcccgt gacagttttc caactcgacc ggctcccatc gggcgtgtgc
    10081 ggcctgtcct cccctctctt ccatctccga ggctcgcgag agccaggggg cgcccagcag
    10141 atgatccagg accgccagcc tggactcacg ggtagggaat agcggctcct cgaaaccctc
    10201 gggtcgcaag gggtagctgt gtatctccga gacgctcgcc tccatctctc tcctccctcc
    10261 ggggagcgcc agggccgggg gatgagaaac ccgcgcctcc ggatataatc cgaagagcag
    10321 gcgtcctcgc ctccttctaa ccctcgcaga ccggtactgg ttggacgccg gccggccagc
    10381 gaggcccggg tgggggcgct cctgctttgc cgttcttctc tttacgctac cacaaactat
    10441 tgtttgcagt ctacggtaaa ataatataaa gtgttatcgc gaggcgttca gaggaggcgc
    10501 gggtggttcc tgagaactgg aagataagga tggagatgct ccggcgcctg gcccgggcga
    10561 acgtgagggg agaggggcct ccgagcgtac gcgaggtggg ggaggccgtg ggcctgagga
    10621 gttcccagac cgcgtacaag cacctcaaaa agctggagga ggccgggtat atcgagcggg
    10681 agggtgggcg ggcgcggggc atcaggctga cggagaaggg ttgggaggct gcgggcgaga
    10741 tgcctttgtt gggtaggatc gctgccggac gcgggctcga ggccgtgacg gtcggggatg
    10801 aggcctactc gcttgcggcg gagctcgtgg gatcccgctt gggcaggcgg cgctaccttt
    10861 tgagggtcgt ggggcagtcc atgatcgggg cccacatagc cgacggggat cttctggtgg
    10921 tagaagaaga cgaagacccg ccagacggcg ccgtcgttgc ggccctcatc gggggtggcg
    10981 aggaggtcac cgtcaagcgg ctctatcgag agggagacct ccttcggctg aggccagaga
    11041 acggggacca cgaggacctc gtcgtgccag ccgaggacgt gtcgatacag ggacgcgtgg
    11101 tctacgtcgt acacccgccc agggggagaa tctgaagtaa caaggcaaca cgccccggct
    11161 ttcgcctccg ctcgcttgta ggcggatgcg cgattcgtgg taggtcagta cctctgcaaa
    11221 tcgcattagc tacgctcctc gccggggaaa caggcagtcc tggcgggtaa agtagtaggc
    11281 gtgaatgggc caacacagac agccggaggt ccggaacggg aagtcttgtt gacccggccg
    11341 gtggtcctgc tgatcctcgt cgcttttgcc gccgtctttg gcttccagtt gctgctctcc
    11401 gtcgtacctc tgtatgcgaa cgaggccggc ggcggcagct ccggtgctgg tctcgcgacg
    11461 gccgccttca tgctctcgac ggtgctgacc cagatccaga tgccccgcat cctggcccgc
    11521 tacggctacc ggcgggtgct cgcagccgga ctcctgttcc tcggggttcc cgcgctgttc
    11581 tacgcttacg cccagacgct cgtacctata ctggccgtta cgttgctgcg tggtgttggg
    11641 ttcgggatcg tcaccgtggt gttcgcggcc atggtcgtcg agctggcccc tgccggccgc
    11701 aggggagaag ctctagggct cctcggcgtc gcattcgcgc tgccgaacat cttctgcaac
    11761 gcgctggggc tctggctggt ggggcagttc ggctacgagg tcgtcttctt gctcgggggt
    11821 gtggctcctc tgcttgggct aggtatggtg atcggcatcc gctccgccgc gccatcgggg
    11881 aggggagagg aggaagaagg cgctggtttc ttcgccggcc tcgggcgttc tcctctattg
    11941 cgcatcttcc ttctctttgc ggccacgaca atggccgccg gggtggtctt gaccttcctg
    12001 cctctggcgg tgcccggctc aggggttttc tccgctgcga gcgcgctcct cgttgtcggt
    12061 atcacctcca ccgccagccg ctggtgggcc gggaggttcg gcgaccgcag ggacccacgc
    12121 ctcctgctgg ctcccggtct cctcgcctgc gcggcgggga tggtctgcct tccgctgggg
    12181 ggtattgtcc tgctaatcgg ggccgtgcta ttcgggacag ggttcgggtt gctccagaat
    12241 gcaaccctca tcctgatgat ggggcgtgtc tcgaaatccg agtacgggct aggcagcaca
    12301 ctgtggaacg cggctttcga tacagggacc gggataggag ccttctcgtt cgggttggtg
    12361 atctccgctg tcgggttctc ctggtccttc ttcatctgct cggcactggt ggcatcagcc
    12421 ctcctactcg tgatcctcga ctattcatcg acacaagact gaccatgatc atcgcgaact
    12481 ccgcgagaaa tacgtgatcc ggaacctagg gttccatccg ctcgacccac tgcgcccgcc
    12541 acatcttccc gtcgcgcagc tcgctgatca gcaccacgtc gaatacctgc ccaccgccgt
    12601 agtcgatgac cgcctcgaca acccacagtc cctccttgac tagcacccgg cgcaactgaa
    12661 gagaccgcgg cggggtggag tggtcgctac gggctctctg gaacgcccgc atgttctccc
    12721 tgccgcggaa gtgctccccg gactggggca tctccatcac atagccttgg tggcgcacgt
    12781 tgtattcctc ttcggggctg agaccaacat ctccctttcc accatacaaa gtgctatcaa
    12841 gatatatcgt gatatactgc ggagctatag ctggtcgccg gaggaaagaa ggaggcgaga
    12901 tgtacggtcg gggcatgatg ggctacacgg gtgggaggcg gacgatggta ccttatggta
    12961 ggccctttcc cccgaggttc atgatggggt tggtgatagg agggttgttt ctgctcttcg
    13021 tggccggtcc cgccttccac ttcttaccgt ttcttttcct actcatctgg gccgtcccct
    13081 ttttcctggc gccggcgttc gtggccgtcg cgcggggctt gtcggggctc tcggagtccc
    13141 ttacgcggaa gggtgggacg gtaacggagg agagcaagga gcgggagctt ctggaggcgc
    13201 tcgcgcggcg tggggaactt tcgcctgcgc tggcggcgct ggagacttcg ctcacagttg
    13261 ccgaggcgaa ccggatgctc tcggatcttg ccaacagcgg tcacctggag gtaagggcgc
    13321 aagaggggag gttggggtat gcgctctggg agcgagaccg gcgcgagttg acggacggag
    13381 cttagtgcgc gtttgcgaag gaggtcgata acatgttcgg tccaaaggcg atgaaggggt
    13441 tcttcggggg gtggcacagg ccggggttcg gccacggtcc gcggcggggg cggatgttcg
    13501 agaaggggga cttgaagtat gtgatcctcg acctgctcaa ggacgaaccc tcccacggct
    13561 acgaggttat acggaagctg gaggagaggt cccgcgggtt ctactctccg agtcccggct
    13621 ccgtttaccc tacactgcaa ctgctcgaag acatggagta cgtgagcgcc acacagcggg
    13681 acggcaagaa agtctacgcc attacggacg aagggcggaa gttcctggat gagaaccaac
    13741 gctccgtcaa cgatatctgg ggcaagttcg gaggaagctg ggatcctggc gtcgcgtccg
    13801 agctgcacga gatcaggcac gacctcatgg acctcggcaa gctcttcggc cagaaaatgc
    13861 gcgagggaag agtggaccag gagaagctgg cccgcataag ggctgtgatc tcagccgcca
    13921 cgagggagat agaagacatc ctgcgagacc gtgacgaagt gcaaagggct tgaacctctt
    13981 tgcatgtcag cacgccgccc gcagggcggc gtgctgacat gctgacatgc tggtatcttg
    14041 ggaggtgtag agaggtcgag caagggggaa cgtcgcatgg cacagcggaa gggagaagaa
    14101 gccgctgggt accggcgcga ggacgacggc gtccaacccc cttacctcta tcctgactac
    14161 gtggcgacgc ggctcagagc cccgaagaag ccgctcatca tcctccccag gacgctctcg
    14221 gacaccacgg gtccggcata cgggcgcggt gctgtagggg accttgacga cgatctgacc
    14281 cgccagcatt ccggcgagcc tctgggtgag aggatcatcg ttacgggacg cgtcctcgat
    14341 ggtgacggac gcccggtaaa gagcagcctc gtggagatct ggcaggccaa cgccgccgga
    14401 cgctatacac accatctgga ccagcaccct gctccactag atccgaactt ctcgggggcg
    14461 ggcagatgcc taacggacgc cgagggccgc tacaggttcg tgacggtaaa gcctggggcc
    14521 tatccctgga agaatcacga caacgcctgg cgcccggcac atatacactt ctcgctcttc
    14581 gggccggcgt tccggagccg cctcataacc cagatgtact tccctggcga cccgctgctc
    14641 gagcacgatc ctatctgcca atccgtacga gatccgaagt ctcgacaacg catgatctcg
    14701 acgttcgacc tcgaaacaac acaacctgaa tgggcccttg gctacaggtt cgacatcgtg
    14761 ctggaaggaa gggacgccac cccgatggag aaccaccgat gagctctgtg ccaacacctt
    14821 cccagacgat aggacccttc ttccacgacg cgctcctcga caagaactac tccgagctcg
    14881 tctcgccgga ccatcctgag ggtataagga tcaaaggaac ggtctacgac ggggccggcg
    14941 gggtcgtgcc ggacgcgatg gtcgagatct ggcaggccga cacggcgggc cgctacgatc
    15001 atcccacgga cgacagggag gacatctccc tacacgaaga cttctcgggc ttcggacgct
    15061 caggcactga tgcaggcggc gagttctcct ttttgacggt caagccagga cccgttcccg
    15121 gtccggacgg aaaggtccag gcaccacacg ttatggtctc cgtcttcgcc cgcggtctcc
    15181 tgaagcgtct ggtgacccgc atctacttcc ccgacgagga agtggctaac gctgtagatc
    15241 ccgttctgtc gtccatcgaa gaccctgagc ttcgg
//
LOCUS       DASTKM010000189         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_404806, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000189 DASTKM010000000
VERSION     DASTKM010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404806"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="VFQ10_07790"
     CDS             <1..1522
                     /locus_tag="VFQ10_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET9965280.1"
                     /translation="IGYALQWGNLMDVGGSTAGSIPIDARSIYEEGVRLPPVKLYDGG
                     TLNEEVLRFFCHNSRTPRETRADVMAIAAGTAAGAQRVKDICDRFGKDTYLEACDALL
                     ARTRTSIINLIRQLIPEGERFEFEDYTDDDGLGNGPIKLKLAMWREGDSLHLDWTGTD
                     AQVPGPVNFLLNKEMFQMFAGVFLISAVDPTILFNDGYADIIKVNIPDGTVLRPREPA
                     PLSNRLVVMARQFDVLGAVYAKAIGFNVSGSYGTSPNFVYSGVKETGEPFQTMEILYG
                     GIPAIPGKDGLDGHSWWPEFLAVPAEYMETYYPLIVEEYSARKDSCGAGQFRGGCGIK
                     KVYRFLADGYITYQDDRHHTYPYGVDGGKPGAASKKTLVRANGEEIEMPAKVRDVPVF
                     EGDRLIFETAGAGGVGDPLERDPEAIAKDVRWSLISPEAAESHYGVVVGDDGSVDAVA
                     TESKREEITASRGESPGFDHGDLPPLDEQRRMIAETRRKFNEWLSGELGKARGNGS"
     gene            1527..2153
                     /locus_tag="VFQ10_07795"
     CDS             1527..2153
                     /locus_tag="VFQ10_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET9965281.1"
                     /translation="MAHIEGFGGRGGFGRRPALVVVDMTLGFTDPESPLSCDLEGPIS
                     EIQKLLRAARRAEIPIVFTTVAYRESDKLTAAAFIDKVPALLTLQAGTRWAEIDPRIA
                     PEETEPVLNKLFASGFFGTGLSSLLTAAGIDTLIITGASTSGCVRATAVDALQYGFRP
                     VVPREAVGDRNPDAHEANLYDIDAKYGDVVPVQEVLDLLEDDAAAHAR"
     gene            2169..>3232
                     /locus_tag="VFQ10_07800"
     CDS             2169..>3232
                     /locus_tag="VFQ10_07800"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9965282.1"
                     /translation="MTGSTFTHIEGALSGESYPADELIGLDPVDERPLLARYDIEKAA
                     RTLTEESLAERRTGGLWRWAELLPVRDRTNVVYLGEGDTPILRQPRLGEALGVQHLST
                     KAEGLNPTGSFKARGMAVAVSRAVELGVTSFIAPSAGNAAGALAAYGAAAGAKVTVLT
                     PVDATLVNKEEVLVMGARLILVRGLISDCGKISTALADRIGAFDVSTLKEPYRVEGKK
                     TMGFELAEQRGWRLPDVIVYPTGGGTGLVGMWKAFEELEAIGLIGSERPRMVSVQSEG
                     CAPIVRAFENGERFAKPWQNAQTRASGIRVPSAVGDFLILDALRQSGGTAVAVGEDSI
                     SEMQTFAGRMGAGYVSPESAA"
BASE COUNT          636 a         1047 c         1014 g          535 t
ORIGIN      
        1 cataggttac gccttacagt ggggcaacct catggacgtt ggcggctcga ccgcggggtc
       61 catccccatc gacgcgcgct ccatctacga ggagggcgtc aggctaccgc cggtcaaact
      121 ctacgacggc ggcactctga acgaggaggt cctgcgcttc ttctgccaca actcccgcac
      181 cccgcgcgag acccgcgccg acgtcatggc catcgccgca gggacagcag caggcgccca
      241 gcgggtaaag gacatctgtg accgcttcgg caaagacacg tatctggagg cctgcgacgc
      301 gctcctcgcg cggacacgca cgagcatcat aaatctcatc cgccagctca tccccgaagg
      361 ggagcgtttc gagttcgagg actacacaga cgacgacggg ctgggcaacg gccccataaa
      421 gctcaagcta gcgatgtggc gtgagggcga ctcgttgcac ctcgactgga cggggacaga
      481 cgcgcaggtc cctggaccgg tcaacttcct cctcaacaag gagatgttcc agatgttcgc
      541 tggggtcttc ttgatctccg ccgtagaccc gaccatcctc ttcaacgacg gctacgctga
      601 catcatcaag gtcaacatcc ctgacggcac ggtcctgcgc cccagggagc ctgcgccctt
      661 gagcaatcgc ctcgtcgtca tggcccgcca gttcgacgtc ctcggcgccg tctacgcgaa
      721 ggccataggc ttcaacgtct caggctccta cgggacgagc cccaacttcg tctactccgg
      781 cgttaaagag accggcgagc cgttccagac catggagatt ctttacggcg ggattccggc
      841 catcccgggc aaggatggcc tcgacggaca ctcctggtgg ccagagttcc ttgccgtccc
      901 cgccgagtac atggagacgt actacccctt gatcgtggag gagtactcgg cccgcaaaga
      961 ctcctgcggc gccggccagt tcaggggcgg atgcgggata aagaaagtgt accgtttcct
     1021 ggccgacggc tacatcacat accaggacga taggcatcac acctacccct acggcgtgga
     1081 cggcgggaag cctggcgctg cgtccaagaa gacgctcgtc agggcgaacg gcgaagagat
     1141 cgagatgccg gccaaggtgc gcgacgtgcc cgtcttcgag ggcgaccgcc tcatcttcga
     1201 gacggccggg gccgggggcg tcggagaccc cctagagcgc gaccccgagg caatagccaa
     1261 agacgtccgc tggagtctca taagccccga ggccgccgag agtcactacg gcgtcgtggt
     1321 cggtgacgac ggatccgtag acgcggtagc gacggaatcg aagcgcgagg agataacggc
     1381 tagccgtggc gagtcgccag ggttcgacca cggagacctg ccgcccctcg atgaacagcg
     1441 gcgcatgatc gccgagaccc gccgcaagtt caacgagtgg ctctcaggcg aactcggcaa
     1501 ggcgcgaggc aacgggagtt gacgctttgg cgcacataga agggttcggc ggacgcggcg
     1561 gcttcggccg ccgtcccgcc ctcgtcgtcg tagacatgac ccttggattc acagatcccg
     1621 aatcgcccct atcctgcgac ctcgaaggcc cgatctcgga gatccagaag ctcctgagag
     1681 cagcccgacg agcggagatc cccatcgtat tcaccaccgt cgcctaccgc gagagcgaca
     1741 aactcaccgc cgccgccttc atagataagg taccagcgct ccttacactg caggccggaa
     1801 ctcgatgggc cgagatcgac cctcgcatag ccccagagga gacggagcca gtcctgaaca
     1861 agctcttcgc atcaggcttc ttcggcacgg gtctctccag cctgctcacc gccgccggta
     1921 tcgacaccct catcatcacc ggcgcatcca cctccggctg cgtccgcgca acggcggtcg
     1981 acgccctcca gtacggcttc cgccccgtcg tcccgagaga agccgtaggc gaccgtaatc
     2041 ccgacgccca cgaggctaac ctctacgaca tagacgccaa gtacggcgat gtagtccctg
     2101 ttcaggaggt gctagatctc ctcgaggatg acgcagcggc acacgctcgt tgaggagttg
     2161 atttgaaagt gaccggttct acgtttacgc acatagaagg cgcgctttcg ggggagagct
     2221 accctgcgga cgagttgatc ggactcgacc ccgtagacga acgccccttg ctcgcccgct
     2281 acgacatcga gaaggccgcc cgaactctga cagaggaatc gctggctgag cgccggacgg
     2341 gcggcctgtg gcgctgggca gaacttctgc cggtgaggga ccggactaac gtcgtgtatc
     2401 tgggagaggg cgatacgccg atcttgcggc aaccacgact tggtgaggcg ctcggtgtcc
     2461 agcacctctc caccaaggca gaaggcctca atcccacagg ctcgttcaag gcgcgaggga
     2521 tggccgtcgc ggtctcgcgg gccgtagagt tgggcgtgac ctcgttcatc gcgccttcgg
     2581 ctggaaacgc tgcgggcgcg ctcgcggcct acggcgctgc tgctggggcg aaggtcacgg
     2641 tgctgacgcc cgtggatgct acgctcgtca acaaggaaga agttctcgtg atgggggcgc
     2701 ggcttatcct ggtgcggggt cttatctccg attgcggcaa gatctcgacc gcgctcgccg
     2761 acaggatcgg cgccttcgac gtgagcacgc tcaaagagcc ctaccgcgtc gagggcaaga
     2821 agaccatggg cttcgaactc gccgaacagc gcggttggcg cttgccggac gtgatcgtct
     2881 accccacggg cgggggcacg ggcctcgtag ggatgtggaa agccttcgaa gagctagaag
     2941 ctatcggcct gatcgggtcc gaacggccaa gaatggtgag cgtgcaatct gagggttgtg
     3001 cacccatcgt aagggccttc gaaaatggag aacgtttcgc gaagccctgg cagaatgccc
     3061 aaacaagggc ctccggtatc agggtccctt cggccgttgg tgacttcctg atcctggacg
     3121 ccctgagaca atccggtggc acggccgtcg cggtgggcga agactccatc tctgagatgc
     3181 agacgttcgc gggacgcatg ggagccggct acgtcagccc cgaatccgcc gc
//
LOCUS       DASTKM010000190         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_934648, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000190 DASTKM010000000
VERSION     DASTKM010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934648"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFQ10_07805"
     CDS             <1..459
                     /locus_tag="VFQ10_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HET9965283.1"
                     /translation="HPKRETRVSCATCGRPICTECMVATDVGIKCPDDARLPRGARAG
                     VMKSDQVLRSILAGVGVAVAGVPIAYVLFLLPFTWLISAAAGYGAGVLINRSGGRNGG
                     PLAIVIAVLATAVPFLVLLAPDLLAGALDPRPLIAMVFAVIAAGVANRQI"
     gene            864..1391
                     /locus_tag="VFQ10_07810"
     CDS             864..1391
                     /locus_tag="VFQ10_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965284.1"
                     /translation="MQYISGVLDELEAIVQDASGVPMSKGRAVVDRSDLLVMLDELRG
                     SLPPELAEAESLRRECAATVSAAEEEAQRIIEEAHHHADAMVPETELFRRTQRRAGEV
                     IDGAERYAEEVSGGSEVYRDRVMGQLENWFQDSLISVEESRQELGGVPVHRPAPRSEV
                     VEEETDGRGWRASSA"
     gene            complement(1472..2269)
                     /locus_tag="VFQ10_07815"
     CDS             complement(1472..2269)
                     /locus_tag="VFQ10_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965285.1"
                     /translation="MSERRNPRSGPPVDRGNAPLSPEERNRMLRAAALRPINVMMLVI
                     GAGVFLTTLVWWLLPLTIVTYASLVFLAARDPIFQRKVLGGGEVAKTSPNAVESSDVS
                     PERRARWLPRGETRQKVEAALDVYRKVVAAIETSDDVTRAVLDDVVPRLHAAADRLVD
                     VARGRERAAEVARDLGEDTGSAAPTTARQENLRELQNRISAADTEISDTYQELLDLRA
                     KVVRISIDTENPARANDLNTSLDGLNARLEALSELMASPGEPPREHD"
     gene            complement(2262..>2765)
                     /locus_tag="VFQ10_07820"
     CDS             complement(2262..>2765)
                     /locus_tag="VFQ10_07820"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET9965286.1"
                     /translation="LGGISAACALTEERFHLHPVSSTFHGRDVFAPVAAHLAAGVGLS
                     DLGKPVDPSSLMRLRPASVFVDEGAGLVTRIISVDRFGNARLSIMHEELGLEYGDALK
                     VDVGDGAMSVRYVETFGSAKAGELVLVPDSHWRLSLAINKGSAAQALALEVGGKVRLI
                     PTTDHGV"
BASE COUNT          522 a          846 c          875 g          522 t
ORIGIN      
        1 catccgaaaa gggagactcg cgtctcatgc gccacgtgcg ggcggcccat ttgcaccgag
       61 tgcatggtgg ccaccgacgt cgggataaaa tgccccgacg atgccaggct accaagagga
      121 gcgcgggccg gcgtcatgaa gtcagatcag gtcctcagga gtattctcgc cggcgtcgga
      181 gtcgctgtcg caggcgtccc catagcgtac gtgctcttcc tcctcccctt cacctggttg
      241 atctcggccg cggccggcta cggggcaggt gttctgatca accgctccgg gggccgtaac
      301 ggcggccccc tggcgatcgt catcgccgtc ctggccacgg ctgtaccgtt cctcgtcttg
      361 ctggctccgg atctcctggc cggcgcactc gacccgcggc cactgatcgc catggttttc
      421 gctgttatcg ccgccggcgt agccaaccgt cagatctaga acgcccagcc atacgacccg
      481 cgccgcctgg atgaaacgcc tcacgataca cccgctcgag agtccaaaac ccttgccgaa
      541 gggccttcgc ccagaccgtc gtcacggtgg gcctcataac agccggaaac tagtgaatcc
      601 ctactctcac ctctcctttt taacgtggtc gacccctgct cctcgaccga acacccctgt
      661 ggataactcg cagaacccca ctaataaagc gaaaaacagg gcttctcacg ttgcggcaga
      721 gatctttact ttcgtgtaac aaagctacac ttaaccttaa gataaaatta aacctgacaa
      781 tgaggtaaag catcgaaaaa tactttcaga aagcctagcg taggctctca gagcgtgcta
      841 gggttggagc catgagagaa gacatgcagt acataagcgg agtattggac gagctggagg
      901 cgatagttca ggatgcatcc ggcgtaccga tgagcaaagg acgggccgtt gtggatcggt
      961 ctgatctgct tgtgatgctg gacgagctga ggggatcttt gccccctgag ctggctgaag
     1021 ccgagtcgct ccgccgggaa tgcgcggcca cggtctctgc ggcagaggag gaggcccagc
     1081 gcataatcga ggaagcccac caccacgctg acgcgatggt tcccgagacg gaactcttca
     1141 ggcggaccca gcgtcgggct ggagaggtca tagacggggc ggagcgctac gctgaggaag
     1201 tctccggcgg ttccgaggtt tacagggacc gcgtcatggg gcagttggag aactggttcc
     1261 aggactcgct catctcggtg gaggagtcca ggcaggagtt gggcggtgtc cccgtgcacc
     1321 ggcctgcgcc ccggtccgag gtcgtcgagg aggagaccga cggtcggggc tggcgggcga
     1381 gctcagccta gcgaagtttg caaagaggtc aaaacctctt tgcaagcatc tcagccagtc
     1441 agcacttcgg ttttggtggg aaagcctcga gttagtcgtg ctctcgggga ggttctcctg
     1501 gcgaggccat gagttcgctg agggcttcga gcctggcatt gaggccgtcg agtgaagtgt
     1561 tgaggtcgtt cgcccgggca gggttttcgg tatctatgga gatccggacg acctttgccc
     1621 tgaggtccag aagctcctgg tatgtatcgg agatctcggt gtctgcggcg cttatcctgt
     1681 tctggagctc gcgcaggttc tcctgtcttg cggtagtcgg tgcggcagag ccggtatcct
     1741 cgccaaggtc acgtgccact tcggcggccc tctccctacc ccgggccacg tccacgagcc
     1801 ggtccgccgc cgcgtgcagc ctggggacca cgtcgtcgag gaccgccctg gttacgtcgt
     1861 ctgaggtctc gatggcggcg acgaccttgc ggtagacatc gagagctgcc tccaccttct
     1921 ggcgggtctc tccgcgtggg agccagcggg cccgtcgttc gggagagaca tcactgctct
     1981 ctacggcgtt cggcgaggtt ttcgcaacct cgcctccccc gaggaccttg cgctggaaga
     2041 ttgggtctct cgcggccagg aagacaaggg aagcgtaggt tacgatggtc aagggcagca
     2101 accaccacac cagggttgtt aagaagacgc cagccccgat cacgagcatc atgacgttga
     2161 tcggacgcag cgccgccgcc cgcagcatcc ggtttcgctc ctcgggcgag aggggagcgt
     2221 tcccccggtc caccggaggg cctgacctgg ggttacggcg ttcagacacc gtgatccgtc
     2281 gtgggtatca ggcgtacctt tcctccgacc tcgagcgcga gcgcttgggc cgcgctacct
     2341 ttgtttatgg cgaggctcag gcgccagtgg gagtcgggga cgagcacaag ttcgcctgcc
     2401 ttggccgacc cgaaagtctc gacgtatcga accgacatcg cgccgtcacc gacgtctact
     2461 ttcagggcgt caccatactc gagccccaac tcctcatgca tgatggagag ccttgcgtta
     2521 ccgaagcggt cgacggagat gatgcgggta acgagacctg ctccctcatc cacgaatacg
     2581 ctcgccggtc tgagacgcat cagtgaagaa gggtccacgg gcttccccag gtccgagagc
     2641 ccaaccccgg ccgcaagatg ggccgctacc ggggcgaaga cgtcgcggcc gtgaaaggtg
     2701 ctggagacgg gatgcaggtg aaagcgctcc tcggtgagcg cacaggcagc cgagatgcca
     2761 cccag
//
LOCUS       DASTKM010000191        15601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_405009, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000191 DASTKM010000000
VERSION     DASTKM010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15601
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405009"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFQ10_07825"
     CDS             complement(<1..251)
                     /locus_tag="VFQ10_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HET9965287.1"
                     /translation="MDKSKAIDAAISQIERQFGKGSIMRMGDEERQSVASIPTGALAL
                     DLALGVGGVPRGRVVEIFGPESSGKTTLALHIIAEAQKAG"
     gene            complement(369..1274)
                     /locus_tag="VFQ10_07830"
     CDS             complement(369..1274)
                     /locus_tag="VFQ10_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HET9965288.1"
                     /translation="MEDWQHKRNPEEDEESRGETEIGRFLEQKRKERGLSLEEVEQAT
                     KIRKRYLTGLERDNYSILPDAVYARGFLKTYANYLGLDGEALSRQLKSSRKARRERGI
                     NYNSGPESDFEKPLITPSGLRGAQKRMVSTSAIVTLIVAVLALAAVIGALYFVGRGVQ
                     VSKEDSPSGKSPPRQEQQNVAGREKAPETGSAKEGAVGSNGTATGDQKPAVAKQSAPP
                     DTLRVLVNVRERPSWILIRTDGTAVYEQVAQPGFSKTFEAEQRLFIKSGDAGAVTVKI
                     NGQDAGPLGGAGEIVARNYTLKTAS"
     gene            complement(1300..3570)
                     /locus_tag="VFQ10_07835"
     CDS             complement(1300..3570)
                     /locus_tag="VFQ10_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET9965289.1"
                     /translation="MATDKRAPKKTTKKRSSGRGKTRRKGGSPLGRVRDGLAGAVTRE
                     AVGVFLLAVGLFFSAAFASGRGAFVGDAGSFVTGQLFGRVGLALAPLSALAGLLLLLG
                     RLRGRAVTGAALLLLAGAATFAATLSPRHLLSAGHYPEAGGILGSGLYAGVQWAAGAI
                     GAALAIALLYAIGLSLLTGVSLAAALAALKRTCEKVVGLGRGLWVKGERRTGSRAKRS
                     HTDAAKGNETADPLGWDADEVVPAELEKRESESAPTREFEVVLPQREPAQAFSEEREL
                     APGEYTPPPFSMLSLGHGNPEHDAEGTSQRLTLALSDLGVVAHVVRAVVGPRVTRYEL
                     RLGSGVKVGKVRNLQQDIAYALAATEVRILAPIPGKSAVGVEVPNTRPAKVTLGDVFQ
                     AYPDANEWTLPVGLGKDISGRAVFFDLSEMPHLLVAGTTGSGKSVMLNGLLTSLLLTT
                     DPRQVKMVLIDPKRVELSQFGRVPHLITPVVTDVKKAANALAWAVAEMERRYEVLEKI
                     GVRSVEGYNDRAETQMPYVVVVIDELADLMMQAGAKVEDAIIRLAQKARAVGIHIVVA
                     TQRPSVDVITGMIKANVPSRIAFAVSSQVDSRVILDSPGAEALLGMGDMLYKPVSAAR
                     PSRVQGAFISEAEVDRVVSATVEAASGRAEARFVKEVTEPRSVKEENDEPDDDLLPEA
                     ASFVVSTQQASVSAVQRRFRVGYSRAGRIIDALERKGIVGPYEGSKSRAVVASAMDLE
                     SMFGDDEGRADLAEGW"
     gene            complement(3636..5297)
                     /locus_tag="VFQ10_07840"
     CDS             complement(3636..5297)
                     /locus_tag="VFQ10_07840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET9965290.1"
                     /translation="MLDNETLQVVPLGGLGEIGKNMTAVRQASGMILVDAGMSFPDEE
                     MPGIDLVLPDFTYVREHAGVLKGIVLTHGHEDHVGALPYLLREFNVPVYATKLTLGLV
                     RSKLQEFGIKRGDLREIKAGDRQNLGGFGLEFIEVNHSIPGAVAIAISTKAGVILFSG
                     DYKIDLTPIEGSPMDLGRLAELGKEGVLAYFGDSTNSERPGYVPSEKIVGDTLNEVFE
                     RSKGRIIVASFASHIHRVSQVVEAAKKHDRLVGVTGRSMIRNVQIARELGYLDLPQEM
                     MVDQRDVNQIPDSDIVVLTTGSQGEPLAALSRIANGTHRTIEVGPEDTVVIAAHPIPG
                     NERGVSRTINNLMKRGAHVFYSPLVPVHVSGHAAQEEQKIVLSLLKPRYLVPVHGEFR
                     HLRHHANTAISLGIPEDKIFILENGDCLEFEDGEARLAGNVAAGMFLVDAGAIAESSS
                     GVMRERQQLSEDGMFIVVARINAQNGQLLGPPDVISRGFVSPQSANGLVDQSIEVVSQ
                     TLEQTASQRITDWGELKNAIRKDLSSFLSERTGKRPIILPLIVEV"
     gene            complement(5363..6619)
                     /locus_tag="VFQ10_07845"
     CDS             complement(5363..6619)
                     /locus_tag="VFQ10_07845"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9965291.1"
                     /translation="MADSNVRLKDLPGGLRVFSEPLAEATSVSLGLWIRAGSRDEMDE
                     VAGISHLMEHMLFKGTPDMDALGVAQAFEGIGAQENAATGEEYTVLYARFLPEHLGRA
                     LDIMSDMVLRPTLADLEREREVIVEEIKMYEDRPDQLADEYLSSLIFHEDPLGRPIIG
                     SAETVRGVDHDTLKDFHSRTYTAPNIFVAAAGKLDQDDVERKVEERLSGLPGGVPFVR
                     EARPTPPDSRFFFRFKETEQYHVSMGSLGIPAKSEDRYAMAALNNVLGGGMSSRLFQE
                     VREKRGLAYAVYSYHQGYSDAGAIKTYVGSTTGNVEEAVRVIVEQLGRVMDEEVEQEE
                     LVRTKQQLKSSTLLALESTSARMNRVGRSVITGAELLTPEEISARIEAVTPQDIARLA
                     KEHLRTDHMYLSAVGPKELDLGRYLN"
     gene            complement(6619..8676)
                     /locus_tag="VFQ10_07850"
     CDS             complement(6619..8676)
                     /locus_tag="VFQ10_07850"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET9965292.1"
                     /translation="MRLDIPVGERSIVLETGKLAKQAGGSVTARLGDTMILSTATRSE
                     APRPGATFLPLSVDIEERMSSAGKIPGGFIKREGRPSERAILTSRLTDRPLRPHFPKN
                     YHYEIQVIGTVLVADQDTPYDTVSMVGASAALALSDVPFEGPIGAVRVGRSLDGEFIL
                     NPTYAQIAESDLDLVVAGTKEAITMVEAGANEVPENVMVEALQIAQDAVKDQAEAIEE
                     WALEHGKEKQEIKDAEENPFLADFRERYFGRIKEGIVQTDRRARHDVLQELREELIEG
                     REEEEIPLITSALSTLEKEAFRALYLEDRKRTDLRGLDEIRPTSAEVQVLPRVHGTGL
                     FTRGETQVLSSLALADLGLAQRLDTIEPLTGKRYMHHYNFPPFATGETGRLGSPRRRE
                     IGHGALAERALMPVIPSEEEFPYALRVISEVLESNGSSSMASVCGSTLALMDGGVPIK
                     APVAGVAMGLVKEDDDYVILSDIQGLEDHMGDMDFKVAGTADGITALQMDMKITGVSA
                     QLLQEALEQAKAGRLQILEILHGAIPEPRAEVSDYAPRVEVIKIPKDKIGMLIGPGGK
                     TINALQEQFGVNISVEDDGTVFVAGEGSISRDAASAINGMMKDVEAGEIYTGKVVKTT
                     NFGAFVELTPGRDGLVHISRLAPGRQRVERVEDVVNEGDRIKVRVLEIDKQNRISLEK
                     LEG"
     gene            complement(8712..8978)
                     /gene="rpsO"
                     /locus_tag="VFQ10_07855"
     CDS             complement(8712..8978)
                     /gene="rpsO"
                     /locus_tag="VFQ10_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET9965293.1"
                     /translation="MAVVEHKEEIIQEHRTHEGDTGSPEVQVAVLTRRIGHLTEHLRE
                     HKHDYHSQRGLLKMVGRRRRLLKYLQNKDVERYRALIAKLGLRR"
     gene            complement(9080..9970)
                     /locus_tag="VFQ10_07860"
     CDS             complement(9080..9970)
                     /locus_tag="VFQ10_07860"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET9965294.1"
                     /translation="MRQVVVALGNFDGVHLGHQEVLRRAVEEGRRRGMKVVAATFHPH
                     PRAVLGAGDPPKLLTPLALRCEMLLAYGADEVWVIPFDAALSKKTPEQFVRDVLIREG
                     RAGVVVVGENFRFGHKAAGEFRDLLRIMREAGGTAVGVQVHGMDGGISSTCIRALISG
                     GEVTEAAGLLGRPYVLRGEVVVGDKRGRSIGFPTANVMPDTDAVIPARGVYAGFVRFG
                     EEEYAACTNVGVAPTFGRTESRVEAYLLDFEGDLYGRVVDVGFVKRIREERRFSGVDE
                     LIGQIRRDVEETRIIAGGLG"
     gene            complement(9958..10923)
                     /gene="truB"
                     /locus_tag="VFQ10_07865"
     CDS             complement(9958..10923)
                     /gene="truB"
                     /locus_tag="VFQ10_07865"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET9965295.1"
                     /translation="MHHAAPPRRGQGRASGNSGRCPRPRGRAITERVLSGGLLLDKPP
                     GITSAKAVSGIKRLLPRKTRIGHTGTLDPLASGLLVLLLGRATRLSRYVTNLDKSYTA
                     TARFGAVSDTLDAEGHITDLDAPLPSEEALRDALPALTGDLLQVPPMASAIKQGGQRL
                     YDLHRRGISVEREARPVRVHRLALAGFDPTAQTAKFEISCSSGTYIRTLIDDLATSLG
                     SGAYLTALRRNSVGHLTVGNAHATQDLLPETLHNHIIPPLDVIAHLPAVAISEETGRD
                     LVCHGRPIERCRVAGSYRVMSGGELLAVYRDDEDVGRAEVVLCAK"
     gene            complement(10836..11798)
                     /locus_tag="VFQ10_07870"
     CDS             complement(10836..11798)
                     /locus_tag="VFQ10_07870"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET9965296.1"
                     /translation="MKTRTKNNTLAEIAEFLKSLDRVAVTTHVGSDGDAIGASAALVR
                     LMRRLGATAVFCHAEGAPEYLGWLLPDEPLRELPPDHDLLVADTSRADRTGVPVPEAG
                     ARLNVDHHEDNPLYAEYNLVSPRAAATAEIVADLYAELEVPIDRPTAEALYVGIRTDT
                     GGFNFRNISPRAHELVADLLRAGVVPAEVNERINRRGSIEQLRIVGVSLANAVRYGEA
                     LISTVENSDYERTGATELDSKESIDQLRTVAGVDVVAHLREVPQGTKGSLRSETVDVG
                     EIARRFGGGGHRLAAGYTTPRRPDEAREELLGILEGVLDLGGGP"
     gene            complement(11776..12132)
                     /gene="rbfA"
                     /locus_tag="VFQ10_07875"
     CDS             complement(11776..12132)
                     /gene="rbfA"
                     /locus_tag="VFQ10_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET9965297.1"
                     /translation="MSGRTRKVESQLKEITSEEVASLSDPRIKGLVTVTGVRVSPDLA
                     HATVFYSVLAEEDDIAAKEGLQSAAGRIQAAVGAQMRLKRTPRLHFEPDPVVERATRI
                     EVALREVRTDEDAHEE"
     gene            complement(12129..12410)
                     /locus_tag="VFQ10_07880"
     CDS             complement(12129..12410)
                     /locus_tag="VFQ10_07880"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET9965298.1"
                     /translation="MFCTVRLELELPFSSSLKDKRQTLRSVKDRLRRKNVSVVESDHQ
                     DLWQRATMEIALAAVSRRAAEQKREELRRALLNYEELSILDWREEFVKL"
     gene            complement(12420..14585)
                     /gene="infB"
                     /locus_tag="VFQ10_07885"
     CDS             complement(12420..14585)
                     /gene="infB"
                     /locus_tag="VFQ10_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET9965299.1"
                     /translation="MSKRVYEIAREQSLESKEVIQRLKEAGVQVKSHSSSVEDNVYER
                     VFGAGPSVETPNGRSAAQEAEAQEARADAERSGHGATMAESPAEEPISGPQQSRPQKK
                     RRRVVIDASARNRGGRPAAPAAPAPSKQPERAFVEEKESESSGVRVEPGATVKDLGDA
                     VGVPPTQIIKVLMGLGEMKTLTQTLSTEEIELVSEELGVEVEIGAVEEPEPVEVTPDD
                     DPADLVEKPPVVTVMGHVDHGKTSLLDRIRRSNVQSGEAGGITQHIGAYQVESDGRKV
                     TFIDTPGHEAFTEMRARGARVTDVVVLVVAADDGIMPQTAEAIEHARAAGVPMVVALN
                     KIDVPNANQDRVLGELSERELVPEVYGGDTVTVPVSAKTGEGIDELLENILVVAELED
                     LKANPKAPASGYVIEGERDPGRGPVATLLLSRGTLQKSDAVLAGTAYGRVRAMLDYMG
                     NRVKEAGPGTPVEILGLSGVPEAGTRFEVVDHERIARDRAQQAEERLRRQELAEGGTR
                     RTLEELLGEGGTQDLNLVVKADVAGSVEALKEALARLSTDEVRVNIVRSGVGALTDSD
                     VMLGSASDGILLGFNVRPTNTAKQVAEREGVEIRTYDVIYKALEDIEAAMRGMLAPEI
                     EERESGTAEVRQTFRVPNVGVVAGCYVTSGEIFRNNRVRVVRDGTVVYEGNIASLKRF
                     RDDVRSVREGFECGVGVENFNDVKEGDVLEFFETVEVPR"
     gene            complement(14594..>15601)
                     /gene="nusA"
                     /locus_tag="VFQ10_07890"
     CDS             complement(14594..>15601)
                     /gene="nusA"
                     /locus_tag="VFQ10_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET9965300.1"
                     /translation="REGAEEEARVVLDEDTGDLRVMKDGEDITPHDFTRIAAQVMRQT
                     FYQRLNEVHNEQLLDEYGERMGDVVTGIVQQAHRRMTLVDLGRVEAILPASEQVPNER
                     YENGQRLKVYLLEIREGGRGPSLTVSRRHEGLLKNLFELEVPEIYDGLVEIRAVAREA
                     GLRSKVAVWSKEAGVDPVGACVGPRGSRVRAVVSELRNEKIDIIQWDPEPARFIAKAL
                     SPARVREVYLDEDEKQAEVIVPDDQLSLAIGREGQNARLAVKLTDWKIDIKPESQATE
                     YEEEEEDWEPDEDSTMHRCRAVLSNGRRCANMALPGSLFCCIPSHQAQAAEFEGMVGR
                     G"
BASE COUNT         2564 a         5178 c         4695 g         3164 t
ORIGIN      
        1 cctgctttct gcgcctcggc gatgatgtgc agggcgaggg tcgtcttgcc gctcgactct
       61 ggaccgaaga tctcgacgac gcgaccccgt gggacgccgc caacgccgag ggcgaggtcg
      121 agggccaggg cgccggtcgg aatggaggcc acgctctggc gctcctcgtc gcccatgcgc
      181 atgatcgaac ccttcccgaa ttgccgctct atctgggaga ttgcggcgtc tatagccttg
      241 ctcttatcca atgcatcctc caaaaagcga gagtgccaac agatcatgta atcggcattt
      301 actgcgcctg attatactgt aaggatcttg ggctcccaag gggtttccgt cctcctgcgg
      361 ggtgcctttc agctcgcggt cttcagggtg tagttcctgg cgacgatctc acccgcgcca
      421 ccgagcgggc cagcgtcctg gccgttgatc ttcactgtca cggcccctgc atcaccactc
      481 ttgatgaaca gtcgctgctc ggcctcgaat gtcttcgaga atcccggctg ggccacctgc
      541 tcgtagaccg cggtcccgtc ggtccggatc agaatccacg agggccgctc tcttacgttg
      601 actagaaccc gcagcgtatc aggtggagca ctctgcttcg caactgcggg tttctggtcg
      661 cctgtggctg tgccattgct gccgacggcg ccctcctttg ccgacccggt ctccggagcc
      721 ttctccctgc cggccacgtt ctgctgctcc tggcgaggtg gggacttccc tgagggtgag
      781 tcctccttcg agacctgcac cccgcgtccg acgaagtaca gagctccgat aacggcggcc
      841 agggcgagta cggcgacgat cagggtcact atggccgagg tggagaccat gcgtttctga
      901 gcccccctca ggccgctggg tgtgattagg ggcttctcga agtcgctttc gggcccggag
      961 ttgtagttga tgccacgctc acgccgcgct tttctgctgc tcttgagctg tcgagagagg
     1021 gcttcgccgt cgaggccgag gtaattggcg taggtcttca gaaaacccct ggcgtagacc
     1081 gcgtcgggta gaattgagta gttatcacgc tcgaggcctg tgaggtagcg cttgcggatc
     1141 ttggtggcct gctcgacttc ctccagggag agaccccgct ccttacgctt ctgttcgagg
     1201 aagcggccga tctcggtctc accccttgat tcttcgtctt cctccggatt acgcttgtgc
     1261 tgccaatctt ccatcgcgct tatgagccta ccataaaagc taccagccct cggccagatc
     1321 ggccctccct tcatcgtcac cgaacatgga ctccaggtcc attgcagaag ccaccacagc
     1381 gcggctcttg gaaccctcgt aggggccgac gatgcccttg cgctcaagcg cgtcgataat
     1441 cctgccggcc cgcgagtagc ctaccctgaa acgccgctgc acagcgctca cggaggcctg
     1501 ctgggtggag acgacgaagc tcgccgcctc gggaaggagg tcgtcatcgg gttcgtcgtt
     1561 ttcctccttg acgctcctcg gttccgtcac ctccttcaca aagcgcgcct ccgcgcgccc
     1621 cgaggccgcc tcgacggtgg ccgacaccac cctgtcgacc tccgcctcgg agatgaaagc
     1681 gccctgcacc cgcgagggcc tcgcggcaga gacaggcttg taaagcatgt cccccatccc
     1741 gagcagggcc tcggcgcctg gggagtccag gatcacgcga ctgtccacct ggctcgagac
     1801 ggcgaaggcg atgcggcttg gcacgttggc tttgatcatt cccgttatta catccacgct
     1861 cggcctctgg gtggcgacca cgatgtgaat gcccaccgca cgcgctttct gggcgagcct
     1921 gatgatggcg tcctcgacct tggccccggc ctgcatcatg aggtcggcca gctcgtctat
     1981 gacgacgact acgtagggca tctgggtctc cgccctgtcg ttgtatccct cgacggacct
     2041 taccccgatc ttctcgagca cctcgtaacg gcgctccatc tcggccacgg cccaggccag
     2101 cgcgttggcc gccttcttga cgtcggttac cacgggcgtt ataaggtgcg gtacccgtcc
     2161 aaactgggag agctcgactc tcttggggtc tatgaggacc atcttgacct gccgcgggtc
     2221 ggtggtcaga agcagagagg tcaacagccc gttgagcatt acgctcttgc cgctccccgt
     2281 ggtgccagcg acgagcagat gcggcatctc ggagaggtcg aagaacacgg cccgccctga
     2341 gatgtccttg ccaagtccta ctggaagggt ccactcgttg gcgtcggggt aggcctggaa
     2401 cacgtctcct agcgttacct tcgccggcct ggtattgggc acttctaccc cgaccgcgct
     2461 cttgcccggg atcggggcga gtatcctgac ctcggtcgcc gccagggcat acgcgatatc
     2521 ctgttgcaga ttcctgacct tgccaacctt gaccccgcta cctaggcgca gctcgtaacg
     2581 ggtgacccgc gggccgacca cagcacgcac aacgtgggca accaccccga gatcggagag
     2641 cgccaaggtg agcctctggc tcgtaccttc ggcgtcgtgc tcgggattcc catgcccgag
     2701 actcagcatg gagaacggcg gcggtgtgta ctcgccgggt gcaagctccc tctcttcgct
     2761 gaaggcctgg gcaggctcac gctggggcaa gacgacctca aactctctgg tcggagcact
     2821 ctccgactcc cttttttcaa gctcggcggg caccacttca tccgcgtccc acccgagggg
     2881 gtcagccgtc tcgttaccct tcgcagcatc ggtatggctg cgcttcgcgc gcgaacccgt
     2941 tcgcctctct cccttcaccc acagccctcg gcccaggcca acgaccttct cacaagtcct
     3001 cttgagcgca gccagcgccg cggcaaggct aacgccggtg agcagcgaca ggccgatggc
     3061 gtagagcagg gcgatggcaa gcgcagcccc gatggcacct gcagcccact gcacgcctgc
     3121 gtagagtcca cttcctagaa tacctccggc ctcgggatag tgcccggcgg aaaggaggtg
     3181 tctgggggac agagtggcag cgaaggtggc cgcgccggcc agcagcaaga gagcggcgcc
     3241 ggtgacggcc cggcccctca ggcggccgag caagaggagc aggcccgcga gcgccgagag
     3301 cggtgcgagc gcgaggccga ccctcccgaa cagctggcct gttacaaagg atcccgcatc
     3361 cccaacgaaa gccccccttc cactcgcgaa cgctgcgctg aagaacaggc caacggccaa
     3421 taagaagacc ccgactgcct ccctggtcac cgccccggca aggccatcac gtacgcggcc
     3481 caggggcgag ccacccttgc gcctcgtctt gcccctgccg gaggagcgct tcttcgtagt
     3541 cttcttgggc gcacgcttgt ctgtagccac gccttaagtc tatcccatga gcctctagtc
     3601 caccgtcaac cgcaaggtgt agtacagggt tagatctaaa cctcaactat tagcggcagg
     3661 atgatcgggc gtttacccgt acgctctgag aggaagctgg aaaggtcctt gcgaatagcg
     3721 ttcttcagtt cgccccagtc tgtgatacgc tggctcgcgg tctgttccag ggtctggcta
     3781 accacctcta ttgactggtc gacgaggccg ttggcgctct gcgggctgac gaagccgcgg
     3841 gagatgacat cggggggtcc gagaagctgg ccgttctggg cgtttatgcg ggccaccacg
     3901 ataaacatac cgtcttcgga gagttgctgg cgctcgcgca tcacgcccga agaactctcg
     3961 gcgatcgcgc ctgcatctac gaggaacatg cctgcggcga cgttcccggc gaggcgggct
     4021 tcaccgtcct cgaactccag gcagtcgcca ttctccagga tgaagatttt gtcctcgggg
     4081 ataccgaggc tgatcgcggt gttcgcgtgg tggcgaaggt gcctgaactc gccgtgcacg
     4141 gggacgagat agcgcggctt gaggagcgag agcactatct tctgctcctc ctgggcggcg
     4201 tgtcccgaga cgtgaactgg cacaaggggg ctgtagaaca cgtgggcgcc ccgcttcatc
     4261 aggttgttga tcgtgcgaga gacgccgcgc tcgttgcccg ggatagggtg tgcggctatc
     4321 acgaccgtat cttcgggtcc cacctcgatg gtgcggtggg tcccgttggc gattcggctc
     4381 agggccgcca ggggctcgcc ctgcgagccc gtcgtaagga ccacgatgtc agagtcgggg
     4441 atctggttga cgtcccgctg gtcgaccatc atctcctgtg gcaggtcgag gtagccgagc
     4501 tcgcgtgcga tctgtacgtt acggatcatc gagcggcccg ttaccccgac gagcctgtcg
     4561 tgtttcttgg cagcctcgac tacctggctg acgcggtgga tgtgtgaggc gaaggaagcc
     4621 acgatgatgc gtcccttcga cctctcgaaa acttcgttca gcgtatcccc gacgatcttt
     4681 tcggagggca cgtagccggg gcgctcggag ttcgtcgagt caccgaagta cgcgagcacc
     4741 ccttccttgc caagctcggc caggcgcccc aagtccatcg gcgagccttc tatcggggta
     4801 aggtcgatct tgtagtcgcc ggagaacagg atcacacctg ctttggtact gatggctatc
     4861 gcgacggcac ctggtatgga gtggtttacc tctatgaact ccagtccgaa gccaccaagg
     4921 ttctgccgat cgccggcttt gatctcgcgc agatctcccc tcttgatccc gaactcctgc
     4981 aacttgctgc gcacgagccc gagggtgagc tttgtagcat agactggcac gttgaactca
     5041 cgcagcagat acggcaacgc gccgacatgg tcctcgtgac cgtgcgtgag cactattccc
     5101 ttgagcacgc ccgcgtgttc ccgcacgtag gtgaagtcag ggagcacaag atctatcccc
     5161 ggcatctcct cgtcagggaa cgacataccg gcgtcgacca ggatcatccc gctcgcctgg
     5221 cgcacggccg tcatgttctt gccaatctca cccaaccccc ccaggggaac gacctgcaag
     5281 gtctcgttgt ctaacaaaag ttcgtataac ctccttttga acgtatcagg tctcaggcat
     5341 caggaaacct gtagcctgac tatcagttca ggtaccttcc caggtcgagc tccttcggcc
     5401 cgacggcgga gaggtacata tggtctgttc tcaggtgctc tttagcgagg cgtgctatgt
     5461 cctgcggggt cacggcctcg attcgggcgg agatctcctc cggcgtcaag agctccgcgc
     5521 ctgttattac gctgcggccg acgcgattca tcctcgcaga ggtgctctcc agggcgagga
     5581 gcgtcgagct cttgagctgc tgtttggtgc gcaccagttc ctcttgttcg acctcctcat
     5641 ccatgaccct cccgagttgc tcgacgataa ccctgaccgc ctcctccacg ttgcccgtgg
     5701 tggagcctac gtaggtcttg atcgcgcctg cgtcagagta cccctggtgg taagagtaca
     5761 cggcgtatgc gagaccacgc ttctcacgca cctcctgaaa gaggcggctg ctcataccgc
     5821 ccccaaggac attgttcaag gcggccatcg cgtaacggtc ttcgctcttc gcaggaatgc
     5881 ccagagagcc catcgagacg tggtattgct ccgtctcctt gaacctgaag aaaaagcgac
     5941 tatcgggtgg tgtcggcctg gcctcgcgca cgaaaggcac tccccctgga aggccggaaa
     6001 gcctctcttc gaccttccgt tcgacgtcgt cttggtcgag cttgccggcg gcggcgacga
     6061 agatgttggg agccgtgtag gtgcggctgt ggaagtcctt tagggtatcg tggtccaccc
     6121 cgcggaccgt ctcggccgac ccgataatcg gacggccgag ggggtcttcg tggaagatca
     6181 aagacgacag gtactcgtcg gcgagctggt cgggcctgtc ctcgtacatc ttgatctctt
     6241 cgactatgac ctcgcgctcg cgttcgaggt cggcgagcgt cggcctcagg accatgtcgc
     6301 tcatgatgtc gagggcgcgc cccaggtgct cgggcagaaa gcgggcgtag agcacggtgt
     6361 actcctcgcc tgtcgccgcg ttctcctggg ctccgatgcc ctcgaaggcc tgggcgacgc
     6421 caagggcatc catgtcgggg gtccctttga agagcatgtg ttccatcagg tggctgatgc
     6481 cggcgacctc gtccatctcg tcgcgggagc cggcccggat ccagagcccc agggagacgc
     6541 tggtagcctc ggcgaggggc tcggagaaga ccctgagacc cccaggaaga tccttcagcc
     6601 ttacgttcga gtcggccact agccctccag cttctcgagg ctgatgcggt tctgcttgtc
     6661 tatctccagg acacgcacct tgatccggtc gccctcgttg acgacgtcct cgaccctctc
     6721 gacccgctgc cttcctggcg cgagacgcga gatgtgcacg agcccgtcgc gccctggggt
     6781 gagctcgacg aaggccccga agttggtggt cttcactacc ttgccagtgt agatctcacc
     6841 ggcctcgaca tccttcatca tcccgtttat cgccgaggca gcatccctgg atatacttcc
     6901 ttcaccggcc acgaagaccg ttccgtcgtc ctcgacggag atgttcacgc cgaactgctc
     6961 ctgtagcgcg ttgatggtct tcccgcctgg tcctatgagc atcccgatct tatccttcgg
     7021 gatctttatg acctccaccc tgggggcgta atcggagacc tcagccctcg gctcgggtat
     7081 ggcgccgtgc aggatctcga gtatctgcaa acgccctgcc ttcgcctgct ccagcgcctc
     7141 ctgcaagagc tgggccgaga caccagtgat cttcatgtcc atctggagcg ccgttattcc
     7201 gtcggccgtg cctgcgacct tgaagtccat gtcgcccatg tggtcctcga ggccctggat
     7261 gtcgctcaag atgacatagt catcgtcctc cttgaccagc cccatagcca caccggcgac
     7321 gggggctttg ataggcacgc cgccgtccat caacgccagc gtcgaaccgc acacgctcgc
     7381 catcgatgag gaaccgttgg actcgagaac ctcggatatt acgcgtagag cataggggaa
     7441 ctcttcctcc gacggaatga ccggcatcag cgcccgctcg gcgagagcgc cgtgcccgat
     7501 ctcacgccgc cgcggactcc ccaggcggcc cgtctcgccc gtggcgaagg gcgggaagtt
     7561 gtagtggtgc atgtaacgct tgcccgtcag cggctcgatg gtgtcgagcc gctgcgcgag
     7621 accgaggtcg gccagagcca gagagctcaa gacctgcgtc tcgcccctcg taaagagccc
     7681 cgtaccatgg acccgcggaa gcacctgcac ttcggctgag gttggcctta tctcgtccag
     7741 cccacgcaga tccgtacgct tgcggtcctc gaggtagagg gcgcggaaag cctccttctc
     7801 gagcgtcgag agggcggacg tgatcagggg aatctcctct tcttcgcggc cctcgatcaa
     7861 ctcttcgcgc aactcctgga gcacgtcgtg gcgggctctc cggtcggtct gtacgatgcc
     7921 ttcttttatc cgcccgaagt accgctcgcg gaagtcagcg aggaaggggt tctcctcagc
     7981 gtcctttatc tcttgcttct ctttgccatg ttcgagagcc cactcctcta tcgcctcggc
     8041 ctggtccttg accgcatcct gggcgatttg cagggcctcg accatgacgt tctcgggcac
     8101 ctcgttcgcc ccagcctcga ccatggtgat ggcttccttc gtccctgcga ccacgaggtc
     8161 caggtcgctc tctgcgatct gggcgtaggt aggattcagg atgaactcgc cgtcgagcga
     8221 acgtccgacg cgcacagccc cgatcgggcc ttcaaaaggc acgtcggaga gggcgagcgc
     8281 cgcggatgcc ccgaccatgc ttacggtatc atacggggtg tcctggtcgg cgaccagaac
     8341 ggtgcctatt acctgtatct cgtagtgata gttcttgggg aagtgcggcc tgagaggtct
     8401 atccgtgagg cgcgaggtca ggatcgcccg ctccgagggc ctaccctcgc gcttgataaa
     8461 gccaccaggg attttgcccg ccgaggacat gcgctcctcg atgtccacgc tcaaaggcaa
     8521 aaacgtcgcg ccggggcgcg gggcctcaga gcgtgtggcc gtggagagta tcatggtgtc
     8581 gccgagcctc gccgtcaccg agccaccggc ttgcttggcc agctttccgg tttcgagcac
     8641 gatcgagcgc tcacctactg gtatatctag tcgcatattg ctttctcctc ttgctattct
     8701 tgtaacccga gctaccggcg taggccgagc ttggcgatga gcgccctgta gcgctcgacg
     8761 tccttgttct gcagatactt gagtaacctg cggcgcctgc cgaccatctt cagcagtcca
     8821 cgctgcgaat ggtagtcgtg cttgtgctcg cgcaagtgct ccgtaaggtg acctatgcgc
     8881 cgcgtgagca ccgcgacctg cacctcggga gaccccgtgt ctccctcgtg cgtacggtgc
     8941 tcttgaatga tctcctcttt gtgctcaaca acagccaact tcttcgttct cctctttatt
     9001 cactactcta ttgtctaacc cgttcgaccc cttatgctac cacatgcatc aattagtatc
     9061 gtgcaccaat ggcccgcggt cagccgagtc caccagctat gattctggtc tcctctacgt
     9121 cgcgccgaat ctgccctatg agctcatcta cgcccgagaa tcttcgctcc tccctgatac
     9181 gcttcacgaa gccgacatct acaacgcgcc cgtataggtc gccctcgaaa tcgaggagat
     9241 aggcctcgac ccggctctcg gttcgcccga aggtgggagc caccccgacg ttcgtacatg
     9301 cggcatattc ctcttcgccg aagcggacga atcctgcgta gactccgcgg gccggtatca
     9361 ccgcatccgt atcgggcatc acgttggctg ttgggaaccc gatcgacctg ccgcgcttgt
     9421 ctccgaccac tacctcgccc ctgagcacgt agggacgtcc aaggaggccg gcggcctcgg
     9481 tcacttctcc accggagatc aaagcccgta tgcatgtgga gctgatcccg ccatccatgc
     9541 cgtgtacctg aacgcctacg gccgtcccgc ccgcttcccg catgatccgc aaaagatccc
     9601 tgaattctcc ggcagccttg tgcccgaaac gaaagttctc gccgactacg accaccccgg
     9661 ccctaccctc gcgaatcagc acatctctta cgaactgctc gggggtcttc ttggacaggg
     9721 cggcatcgaa cggtatcacc cacacctcat cggccccgta agcgagtagc atctcacacc
     9781 gcaacgccag gggtgtcaag agcttaggcg gatcaccagc ccccagtacg gcccgtggat
     9841 gcggatggaa ggtggcagca acgaccttca tgcccctacg ccgcccttcc tcgaccgccc
     9901 gcctcaggac ctcctggtgg ccgaggtgca caccgtcgaa gttcccgagc gctaccacta
     9961 cttggcgcac aacaccacct cggcccgccc cacgtcctcg tcgtccctgt acacagccaa
    10021 gagctcgccc ccactcatca cccggtaact gccagcgacc cgacacctct ctatgggcct
    10081 gccgtgacat accagatctc ggcccgtctc ctccgaaata gccaccgcag ggagatgggc
    10141 gatcacatcg agtggtggta ttatgtggtt atgtagggtt tccgggagga gatcctgggt
    10201 ggcatgggcg ttgcctacgg tgaggtgtcc gacgctattg cggcgcaatg cggtaaggta
    10261 ggcgcctgag ccgagggatg tggcgaggtc gtctatgagg gttcttatgt aggtaccgct
    10321 gctgcaggag atctcgaact ttgccgtctg tgctgtgggg tcgaagccgg cgagagcgag
    10381 tctatgcacc ctgacgggac gtgcctcccg ctcaacgctt atgccgcggc ggtgcaagtc
    10441 gtaaagcctt tgacctcctt gcttgatggc cgaggccatc ggtggaacct ggaggaggtc
    10501 gcccgtgagg gctgggagcg cgtcgcgcag ggcttcttcc gaagggaggg gggcgtccag
    10561 gtcggtgata tggccttctg cgtcgagcgt atccgagacg gcgccgaagc gggctgtggc
    10621 tgtgtaggac ttgtcgaggt tagtcacgta gcgagagaga cgcgttgcgc gcccgagaag
    10681 gagcacgaga aggcctgagg ccagggggtc gagggttcct gtgtgcccta tcctggtctt
    10741 cctcgggagc aggcgcttga tcccagagac cgccttggcg ctcgttatgc ctggcggctt
    10801 gtccagcaga aggccgcctg agaggaccct ctccgttatg gcccgccccc gaggtcgagg
    10861 acaccttcca gaattcccag aagctcttcc ctggcctcgt cggggcggcg cggcgtggtg
    10921 tatccggcgg cgaggcggtg gccgcctccg ccgaagcgac gcgctatctc cccgacgtcg
    10981 accgtctcgg aacgtagtga gcccttcgtg ccttgaggga cctcgcgcag gtgggccacg
    11041 acgtcgacac cggcgacggt gcggagctgg tcgatggatt ctttcgagtc gagttccgtt
    11101 gcgccggtcc tctcgtagtc cgagttctcg accgtggaga tcagtgcctc cccgtaacga
    11161 acggcgttgg cgagcgagac gccgacgatc ctgagctgtt cgatgctgcc cctgcggttg
    11221 atgcgctcgt tgacctcggc aggcacgacg ccagcccgaa ggaggtcggc gacgagctcg
    11281 tgggcacggg gggagatgtt acggaagttg aagccgcccg tgtcggtcct gatgccgacg
    11341 tagagggcct cggcggtcgg cctgtcgatg ggtacttcga gctcggcgta aaggtcggcg
    11401 acgatctcgg ccgtcgcggc ggccctcggg ctcaccaggt tgtactcggc gtagagaggg
    11461 ttgtcctcgt ggtggtcgac gttgaggcgg gcgcccgctt caggcacggg tacgccggtt
    11521 cggtctgcgc gggatgtgtc ggcgacgagc aggtcgtgat ccggcggaag ctcgcgcagg
    11581 ggctcgtcgg gcaagagcca gcccagatac tcgggcgccc cttccgcgtg gcagaatacg
    11641 gctgttgccc ccagccggcg catgagacgc acgagggcag ccgaagcgcc gatggcgtcg
    11701 ccgtcggaac ctacgtgagt cgttacggcg acgcggtcca gggacttcaa aaactccgct
    11761 atctccgcta gcgtattatt cttcgtgcgc gtcttcatct gtccttacct ccctaagtgc
    11821 gacttctatc ctggtggcac gctcgacaac ggggtcgggt tcgaagtgca gcctgggcgt
    11881 gcgcttgagg cgcatctggg cccctaccgc ggcctggatg cggccggccg cgctctgcag
    11941 gccttccttt gcggcgatat cgtcctcctc ggcgagcacg ctgtagaaca ccgtcgcgtg
    12001 ggcgaggtcg gggctcaccc tgacgccggt aaccgtaacg aggcccttga tgcgcgggtc
    12061 cgagagagag gcgacctcct cgctggtgat ctccttcagc tgcgattcca ccttgcgcgt
    12121 gcgaccactc acagtttgac gaactcctcg cgccagtcca gtatggagag ttcttcgtaa
    12181 ttgaggagcg ccctgcgaag ctcttctcgc ttctgctccg cagcccggcg cgagaccgca
    12241 gcgagtgcga tctccatcgt tgcgcgctgc cagaggtcct ggtgatccga ctccaccaca
    12301 gagacgttct tgcggcgcag gcggtctttg acggagcgta gcgtctggcg cttatccttc
    12361 aggctcgaag agaaaggcag ctccagttcc agccttaccg tgcagaacaa aagccgactt
    12421 tacctcggga cctcgacggt ctcgaagaac tcgagcacgt ctccctcttt gacgtcgttg
    12481 aagttctcga cgccaacacc gcactcgaaa ccctcgcgca cggagcggac gtcgtccctg
    12541 aagcgtttca gggaggctat gttgccctcg tagaccacgg tgccgtcgcg cacgacgcga
    12601 acgcggttgt tgcggaagat ctccccgctc gtaacgtagc agccagcgac gactccgacg
    12661 ttgggcaccc tgaaggtctg gcgcacctcg gcggtaccgc tctcgcgctc ctctatctcc
    12721 ggggcgagca taccgcgcat ggcggcctcg atgtcttcga gggccttgta gatcacgtcg
    12781 taggtccgga tctcgacgcc ctcccgctcg gcgacctgct ttgccgtgtt tgtgggcctg
    12841 acgttgaaac cgagcaggat gccgtcgctg gccgagccga gcataacgtc agagtcagta
    12901 agggccccga cgccactgcg cacgatgttg acgcgcacct cgtctgtgga gagtctggcg
    12961 agcgcctcct tgagcgcctc cacggatccg gcgacatccg ccttgacgac gaggttgagg
    13021 tcctgggtgc cgccctcgcc gaggagctcc tcaagcgtgc ggcgcgtacc gccctcggcg
    13081 agctcctgcc ttcgcagtct ctcctccgcc tgttgggcgc ggtcgcgcgc gatccgctcg
    13141 tggtcgacga cctcgaagcg ggttcccgcc tcggggacgc cagagaggcc gaggatctcg
    13201 accggtgttc ccggtcccgc ctccttgacg cggttaccca tgtagtcaag catcgccctt
    13261 accctgccgt aggccgttcc cgcgagcacg gcatcgctct tctggagcgt cccccgactc
    13321 aacagtaacg tggccacagg accgcgtcca gggtcgcgct ccccctctat aacgtatccg
    13381 ctggcgggag ccttggggtt cgccttgagg tcttctagct cggcgacaac caggatgttc
    13441 tccagaagct catcgatacc ttcgccggtc ttggccgaca ctggcaccgt cacggtatcc
    13501 ccaccgtaga cctcgggtac gagctccctt tcggagagct ccccgagaac cctgtcctgg
    13561 ttcgcgttcg gcacgtcgat cttgttgagg gccacgacca tggggacgcc ggcggccctg
    13621 gcgtgctcga tggcctctgc cgtctggggc atgatgccgt catcagcggc tacgacgagc
    13681 acgaccacgt ccgttacccg cgcaccgcgg gctcgcatct ccgtgaatgc ctcatggcca
    13741 ggggtgtcta taaaggtaac cttcctgccg tctgactcga cctggtaggc cccgatgtgc
    13801 tgcgtgatac cgccggcctc cccggactgg acgttagacc gtcggatcct gtccaggagg
    13861 ctcgtcttgc cgtggtcgac gtggcccatc accgttacta cgggcggctt ttcgaccagg
    13921 tcggcagggt cgtcgtcggg tgtgacctcg accggctcag gctcctcgac cgcgccgatc
    13981 tcgacctcca caccgagctc ttcggagaca agctcgatct cctcggtcga cagggtctgg
    14041 gtgagcgtct tcatctcacc gagacccatc aagaccttga tgatctgggt cggagggacc
    14101 cctacggcgt cgccgaggtc cttgaccgtc gcccccggct cgacccgcac cccgctgctc
    14161 tcagactctt tctcctcgac gaaagcgcgc tcgggctgct tgcttggcgc cggggccgca
    14221 ggcgccgccg gacgcccccc acgattcctc gcactagcgt cgataacgac tctgcggcgt
    14281 ttcttctgag gacggctctg ctgcggcccg gagatgggct cttcggccgg cgactcagcc
    14341 atggtggcac cgtgtcccga gcgctccgca tccgcgcggg cctcctgcgc ctcggcttcc
    14401 tgggccgccg agcggccgtt gggcgtttcg acgctgggcc ccgcgccgaa gaccctttcg
    14461 tagacgttat cctcaacgga ggaggagtgg ctctttacct gaaccccggc ctccttgagg
    14521 cgctgtatca cttctttgct ctccagcgac tgctcgcggg ctatctcgta cacgcgttta
    14581 ctcatcagat cccctaccct ctacctacca tgccctcgaa ctccgcagcc tgcgcctggt
    14641 gcgaagggat gcagcagaac agcgagcctg gcaatgccat attggcgcac ctgcgcccgt
    14701 tggagagaac cgccctgcag cggtgcatcg tggagtcctc gtcaggctcc cagtcctctt
    14761 cctcttcctc gtactcggtc gcctggctct cgggctttat atctatcttc cagtccgtca
    14821 gctttacggc gaggcgggcg ttctggccct cgcggccgat agccagcgac aactggtcgt
    14881 cgggtacgat cacctccgcc tgcttctcgt cctcgtccag gtagacctcg cgtacgcgcg
    14941 ccgggctcaa ggccttcgct ataaagcgcg caggctccgg gtcccactgg atgatgtcga
    15001 tcttctcgtt cctgagctcg gagaccacag cccttacgcg gctaccgcgt ggtcccacgc
    15061 aggcacccac gggatctaca ccggcttcct tggaccacac cgccaccttc gagcgcagcc
    15121 cggcttcgcg ggcgactgcc cttatctcga cgaggccgtc gtagatctct ggcacctcga
    15181 gctcgaagag gttcttgagc agcccctcgt ggcgccggct cacggtcagg ctggggccac
    15241 gccctccctc cctgatctct agcaggtaca ccttcaaccg ctgtccattc tcgtagcgct
    15301 cattgggcac ctgctcgctg gcaggcagga tagcctcgac ccttccgagg tcgaccagcg
    15361 tcatccgtct gtgggcctgc tgtacgatgc cggtcacgac atcgcccatg cgctcgccgt
    15421 actcgtccaa gagctgctcg ttgtgaacct cgttcagacg ctggtagaac gtctgacgca
    15481 tcacctgggc cgctatacgc gtgaagtcgt gcggggttat gtcctcgccg tccttcatga
    15541 ccctgagatc gcctgtgtcc tcgtcgagga ctaccctcgc ctcctcctca gccccctcgc
    15601 g
//
LOCUS       DASTKM010000192         5562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1463479, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000192 DASTKM010000000
VERSION     DASTKM010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463479"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VFQ10_07895"
     CDS             complement(<1..1551)
                     /locus_tag="VFQ10_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HET9965301.1"
                     /translation="MSVFLRRWRIPQPYRAWIDDLMAEQQGQGYRWYAFDNKDSAASR
                     RILEASQEEQRGFVLAAAEWLNLRHGKQTVAFYETWAVRQTMLTVLRRRLPFHHDDVC
                     RLLDWSIRQPYTHVRGTPQMIKVLQDHLKEHELTPDLRKRVTKLTRCLEKGHTTEETR
                     RWAARLRELGGLSSSTIPLVPGEAWSDVAISDIEAMEGEEIAAWVALLQHCTRARGAK
                     PGQKWSTKAESLLEKLGRPAFERSVVRWFPLVERPRTRPVERGSQWQPDPNLLLQDEN
                     ADILKALAWLCAGSENAEVARALGALALTAYRKVPQMGPRCVRLGNACVWALGAMPDA
                     GGVGQLAMLRARVKFGTAQKGIEKALDTAAGQAGMPLQEIEEISVPAYGLQEVGVRRA
                     QLGEFGVELVVRGTSTTELRWIKPDGKRQKSVPKAVKEHSAEDLKEIKAAATDLRKML
                     PAQRDRIENLYLEQKSWTYQAWRQRYLDHPVVGTLARRLIWKFTNEERTGAGVFYNGR
                     IVGRDGHVL"
     gene            complement(1548..1952)
                     /locus_tag="VFQ10_07900"
     CDS             complement(1548..1952)
                     /locus_tag="VFQ10_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965302.1"
                     /translation="MSTTLKIKDETTFSLGGDDDRDFTLDVPDEKISVQELIRARVYR
                     EVHDYNLHQPEYFHGLVQPSEAERSLNGFRMRRRRKIDPERQFELAKRAFYSNGFILL
                     VDDRQVDELEDEIEIRPDTTVTFLKLVPLVGG"
     gene            complement(2046..3422)
                     /locus_tag="VFQ10_07905"
     CDS             complement(2046..3422)
                     /locus_tag="VFQ10_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9965303.1"
                     /translation="MRATRKEAIATVEVPPGPRGNVVLGSIGDLYRDRLRFVLDVART
                     YGDVAQYRVAHMRMYQVNHPAGVGRLLHDNHRNYSKDVATFGTLRLILGNGLLTSDGD
                     FWRRQRRLAQPAFHQRRVTAFGDLMTDATLAMLERWRPSVAQDQPLDVAAEFMHLTLE
                     VATRALFSASVERDVDAIGRAITTLLNEVTFRFTFPFYPPLKVPTPRNRRFLAARATL
                     DGIIYGIIAERRRQPGEHDDLLALLMEARDEKTGEGMSDRQLRDEVITLFVAGHETTA
                     NALSWSSYLLSTHVAVARKMRAEVDEALQGRIPTAADLSNLPYTRMVIDETLRLYPPA
                     WITNRRAIEDDTICGYRIPADAIVSISPYVTHRDPTLWQNPEGFDPDRFAPERSSGRP
                     HFAYFPFGGGPHQCIGKGFALLEATLVLALLTQQYELHLVPGRRVETVAMATLRPRYG
                     MWMTAHPR"
     gene            3775..4566
                     /locus_tag="VFQ10_07910"
     CDS             3775..4566
                     /locus_tag="VFQ10_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9965304.1"
                     /translation="METHVLFFLAAVAAGLINSLAGGGGLITFPLLTLVVPPVVADAT
                     SALALLPAYPSAVWRTRSKLRDVRRLWLWLLLVTSTIGGLVGALLLVWTDDQNFTFLV
                     PWLVLGGTVLFALEPWLSRFSGRAHQDRGLATALWPLAAVVVFVVAIYGGYFGAGIGI
                     LLISALSLLRMGDVHRVVPFKNLLAGCLRGVAVVVLVFYGEIHWGYGVPMAVGGLVGG
                     YLGGALTDRVNRTVLRSVVMIIGLGLSAYFFWTLYGRSVLHIFGE"
     gene            complement(4650..5105)
                     /locus_tag="VFQ10_07915"
     CDS             complement(4650..5105)
                     /locus_tag="VFQ10_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9965305.1"
                     /translation="MPRRMTEKERQEFLAEPHIGVLSVAVGNDRPPHTTPIWYGYEPG
                     GSITFFTETQGRKSRKARYIERAGVLSLTVQREEFPYKYVTVEGSVVGSHRPPSADQM
                     LAIAGRYLPEGAAQGFVEAELAQPGTGPVLFTVRPDRWLTFDFAEGDES"
BASE COUNT          932 a         1743 c         1719 g         1168 t
ORIGIN      
        1 caggacgtgg ccgtcacggc ccacgatccg gccattgtag aagacgccgg ctcctgtgcg
       61 ttcttcgttc gtgaacttcc agatcaagcg ccgcgcgagc gtgccgacga cgggatggtc
      121 gagatagcgc tggcgccagg cttggtaggt ccaactcttc tgctccagat acaggttctc
      181 tattctgtcg cgctgggccg gcagcatctt ccgcaggtcc gtggccgccg ccttgatctc
      241 tttcaaatcc tcggcgctgt gttctttgac ggccttgggc acggatttct gccttttgcc
      301 atcaggcttg atccaacgta gctcagtcgt gcttgtaccc ctgactacca gctcgacgcc
      361 gaactcgccc aactgcgcgc gcctcacccc cacctcctgc aggccgtaag ctgggacgga
      421 gatctcctcg atctcctgga gcggcattcc ggcttgccct gctgcggtgt ccagcgcctt
      481 ctcgatgccc ttctgcgccg tgccgaactt caccctggcc cgtaacatcg cgagctgccc
      541 tactcccccc gcgtccggca tggcgcccaa cgcccacacg catgcgttgc caagcctgac
      601 acacctcggc cccatctgcg gcaccttgcg atacgcagtg agcgccaacg cccccaacgc
      661 tcgcgcgacc tccgcattct cgctccccgc gcacagccag gcgagcgcct tgaggatgtc
      721 cgcgttctcg tcctgcaaga gcaggttggg gtctggctgc cattgtgacc cccgctcgac
      781 gggtctggtg cgcggcctct ccaccagggg aaaccagcgc acgacggacc tctcgaaagc
      841 agggcgtccg agcttctcta gcaacgattc cgcttttgtt gaccacttct ggccgggctt
      901 cgcgcctctt gccctggtgc agtgttgcag aagcgcgacc caggccgcaa tctcctcgcc
      961 ttccatagcc tcgatgtcgc ttatcgcaac atccgaccac gcctcgccgg gtacaagggg
     1021 aatggtcgaa ctcgaaagcc ccccaagctc tcttagcctc gctgcccagc ggcgcgtctc
     1081 ttctgtagtg tgcccctttt cgagacatct agtgagtttg gtgacccgtt tgcggagatc
     1141 tggggtgagc tcgtgctcct tcaggtgatc ctgaagcacc ttgatcatct gtggtgttcc
     1201 tcgcacgtgc gtataaggct ggcggatgga ccaatccagg aggcgacaga cgtcgtcatg
     1261 gtggaagggg agcctgcgtc tcagcacggt cagcatggtt tgacggaccg cccaggtctc
     1321 gtagaaggcc acggtctgtt tcccgtgacg gagattcaac cactccgccg cggccaggac
     1381 gaaccctcgc tgctcctcct ggcttgcttc caggatccga cggctggccg ctgagtcttt
     1441 gttgtcgaag gcgtaccacc ggtatccctg gccttgctgc tcggccatga ggtcatcgat
     1501 ccaggccctg tatggttgcg ggatgcgcca cctgcggagg aatacgctca tcctccgacg
     1561 agtgggacga gcttcaggaa cgtgaccgtg gtgtcgggcc tgatctcgat ctcatcctcg
     1621 agctcgtcca cctgcctgtc atcgaccagc aggatgaacc cgttgctgta gaacgccctc
     1681 ttggccagct cgaactgtct ttcggggtct atcttccgcc tcctgcgcat cctgaagccg
     1741 ttgagggacc tctcagcctc ggacggctgg acgagaccgt ggaagtactc gggctggtgg
     1801 aggttgtagt cgtgcacctc ccggtagacg cgggcccgga tcaactcctg caccgagatc
     1861 ttctcgtcag gtacatcgag cgtgaagtct ctgtcgtcat caccgccgag gctgaaagtc
     1921 gtttcgtcct tgatcttcag cgtcgtagac atcgtcacac tccgtcctcg aaacctactg
     1981 gaaacgtata cgaagcgtac ggcttgttgc acactacgac aagggccaaa aacgactcgt
     2041 ctcgttcagc gcgggtgggc cgtcatccac ataccgtagc ggggacgcag tgtggccatc
     2101 gccaccgtct ctacccgccg accggggacg agatgtagct cgtattgttg cgtcagcagg
     2161 gccagtacga gcgtcgcttc cagtagcgcg aagcctttgc cgatgcactg gtgcggtcca
     2221 cctccaaacg ggaagtacgc gaagtgcggg cgcccggacg aacgttcggg ggcgaacctg
     2281 tcggggtcga agccttcggg attctgccac agtgtgggat cgcggtgtgt gacgtagggg
     2341 ctgatggaga ctatggcgtc ggcggggatg cggtatccac atatggtgtc gtcctcgatc
     2401 gctcgccggt tggtgatcca ggccggcggg tacaaacgca gcgtctcgtc aataaccatg
     2461 cgcgtgtaag gcagattgga caagtcggcg gccgtgggga tgcgcccctg gagcgcctca
     2521 tcgacttcgg cacgcatttt gcgcgcaacc gccacgtgtg tggagaggag gtaggaggac
     2581 cagctgaggg cgttggccgt cgtttcgtgg ccagctacga atagagtgat cacctcgtcg
     2641 cgcagttgcc tgtcgctcat cccctctcct gtcttctcgt cgcgcgcctc catgaggagc
     2701 gccagcaagt cgtcgtgttc gcccggttgc cgccgcctct cagcgatgat gccgtagatg
     2761 atgccatcga gggttgcgcg ggctgccagg aaacggcggt tgcggggtgt cggcaccttg
     2821 agcggtgggt agaaggggaa agtgaaacgg aaggtcactt cattcaagag cgtcgtgatc
     2881 gcgcggccga tcgcatccac gtcgcgctca acagaggcgc taaacaatgc ccttgtggcg
     2941 acctccaacg tcaggtgcat gaactccgcc gccacgtcga gtggctgatc ctgcgccacg
     3001 ctcggtcgcc agcgctctag catcgccagg gttgcatcgg tcatcaagtc gccgaaagcg
     3061 gtgacacgcc gctggtggaa ggcgggctgc gccagacgac gttggcgccg ccagaaatcg
     3121 ccgtcgctgg ttaaaagccc gttgcccagg atcaacctga gtgtcccgaa cgttgcgacg
     3181 tccttggagt agttgcggtg gttatcgtgt aatagtcgcc cgacacccgc cggatggttg
     3241 acctgataca tccgcatgtg cgcaacccga tactgcgcca cgtcgccgta cgtgcgtgct
     3301 acgtcgagca cgaagcgcag gcggtcgcga tagagatcgc cgatgctgcc aagcacgacg
     3361 tttccgcgcg gcccgggcgg cacctctaca gtggcgatgg cctctttcct cgtggcgcgc
     3421 atctgtgccc tccctaatag cttgcgttgg gccgctcgcc ccgcctctct ttctatctct
     3481 cataacacta ctctaccgga cggtcgcttc ctcaacgtca ccacaagaga cgttttcaac
     3541 agcctgatct tcgcagcgat ggtcatgact tgccgagatt tgggcccggg agcaggaacc
     3601 acaactggat gctgagccag gcgggccttc acatctccct ctttgctccg gcgcagtagc
     3661 aggcatcaat gagcattttg gagatgcacc ggaattctcg aggtgagatc atcggcaagt
     3721 tggccatgag ggggacttcg ggcgatggcg aggcacgagg ggagctaaag ccgcttggaa
     3781 acacatgttc tgttcttttt ggccgcggtg gcggccggcc tgatcaactc gcttgccggc
     3841 ggtggcgggc tgataacgtt cccactgctg accctggtcg ttccgcccgt agtcgccgac
     3901 gcaacgagcg cactcgccct gctccccgcc tacccaagcg ccgtatggcg tacccgcagc
     3961 aagttgcgcg acgtacgcag actgtggctt tggttgctcc tcgtcacgag cactattggc
     4021 gggctggttg gagcactgtt gctggtctgg acggacgacc agaatttcac gttcctcgtg
     4081 ccctggttgg tgctcggcgg caccgtcttg ttcgcgctgg agccgtggct ctcccgcttt
     4141 agcgggagag cccaccaaga ccgcggcctc gcgacggcgc tgtggccgct tgcggcggta
     4201 gttgtgttcg tagtggccat ctacggcggc tacttcgggg ccggtatcgg catcttgttg
     4261 atcagtgccc tgagtttgct gcggatggga gacgtccacc gcgtcgtgcc tttcaagaat
     4321 ttgctcgcgg gttgcctcag gggcgtggca gtggtcgtgc tcgtgttcta cggcgagatc
     4381 cactggggat acggcgtgcc aatggccgtc ggcggtctgg tcggcggcta cctaggaggt
     4441 gcgctcacgg acagggtaaa ccgcaccgtc ctgcgttcgg tggtcatgat tattgggctc
     4501 ggcttgtcgg catacttctt ctggaccctc tacggccggt cggtactaca catcttcggc
     4561 gagtaaaccc gaccggattc cgagaagccc ctcgccgcgg cagcgtggcc gttcgtgaag
     4621 taggtgcgaa gggcctcttc gccgtcgcgc taactctcgt caccttccgc gaagtcgaag
     4681 gtcagccagc ggtctggccg gaccgtgaag agcacaggcc ctgttcccgg ttgagcgagc
     4741 tccgcctcaa cgaagccctg ggcagccccc tcgggcaggt accgtccggc gatggcgagc
     4801 atttggtccg cggaaggagg acggtgggac ccgacgacgg agccctcgac ggtgacgtac
     4861 ttgtatggga actcttcgcg ttgcaccgtc aggctcagca cacccgctct ctcgatgtac
     4921 cgggccttgc gggacttgcg gccctgtgtt tctgtaaaga acgtgatgct tccgcccggc
     4981 tcgtagccgt accagatcgg tgtggtatgt ggtggcctgt cgttgccgac ggcgacgctc
     5041 aggaccccga tgtgcggctc ggcgaggaac tcctggcgct ccttctcggt catccgacgg
     5101 ggcatatgtc ctcctccaaa gtagcttccc cgatcctcct gtggatggta gggtcccgcg
     5161 cacgcattca caagtgctta cccgagggtc gcggcccacg tgccagaaag caagtcatcc
     5221 tctccccaag atccttcgga gctacatagc tgggtgtcgc gaatgcggct actggaagag
     5281 aaaggaccgg gtgagcgaga tggtctttgc ccagtcagcg caccagaagt cgcagcggcg
     5341 gtgtgcggat tcccttggtc gaacgaccgt cgatcgaatg agcagttcca tcttgtacta
     5401 tgatgctgct cgtgcaccga gatctgcatc gagatgtcga cggcggtatc tcctcggctg
     5461 tccatggagc gtaaggacgg atcgatgccc gacgagcata gcgaggtttc gatgagtgag
     5521 ttcatgcgaa tctccggcct gcacctggag gaagtcaatg gg
//
LOCUS       DASTKM010000193        24451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_53528, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000193 DASTKM010000000
VERSION     DASTKM010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 24451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24451
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53528"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /gene="sufB"
                     /locus_tag="VFQ10_07920"
     CDS             complement(<1..1301)
                     /gene="sufB"
                     /locus_tag="VFQ10_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET9965306.1"
                     /translation="MPEDKTIQELGLDEYKYGFRDPEEYFFRTPRKGIDPEIVAMISK
                     HKNEPEWMLEFRLKALESFLEKPTPTWGGDLSGMDYDDIYYYIRPMENQGRSWDDVPD
                     DIKNTFEKLGIPQAERESLAGVGAQYESEVVYHSLKKELEEQGVIFLDMDSGLREHED
                     IVEEYFGTIIPPDDNKFAALNSAVWSGGSFVYIPPGVSVDIPLQAYFRINAENMGQFE
                     RTLIIADEDSYVHYIEGCTAPTYTSNSLHSAVVELIAKPGARIRYSTIQNWSHNTYNL
                     VTKRGVAYENATIEWVDGNLGSKLTMKYPAVYLMGEGARGEILSVAYAGDGQHQDAGG
                     KVVHAAPNTSSLITSKSISKGTGRSTYRGLLKVHEGAVNSKSRVECDALLLDENARTD
                     TYPYIEIEEKKVSTEHEATVSKVGEDQLFYLMSRGLSEEEA"
     gene            complement(1317..2069)
                     /gene="sufC"
                     /locus_tag="VFQ10_07925"
     CDS             complement(1317..2069)
                     /gene="sufC"
                     /locus_tag="VFQ10_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET9965307.1"
                     /translation="MLKIEDLHVEIDGQEIVKGLDLEVGKGEIHAIMGPNGSGKSTLA
                     NVLMGHPRYEVTEGSITFQGEDVFELEPDERAKLGMFLAFQYPSEVPGVSVANFLRTA
                     VNSVREEQLNPMEMYRLLQEKMSIMHMDPKFAERYLNEGFSGGEKKRNEILQMLMLDP
                     KLAIMDETDSGLDIDALQVVAKGVNEMKGPEFSAVIITHYQRILRYIEPDRVHVMLDG
                     RLVTSGGKELADDLEEKGYDWVRQEFGAGAQS"
     gene            complement(2363..3367)
                     /locus_tag="VFQ10_07930"
     CDS             complement(2363..3367)
                     /locus_tag="VFQ10_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965308.1"
                     /translation="MKYLKGPQNNADPNDLEGYAAHREGAAVVDRSDRRLLRLTGKDP
                     IGMLNAVLTNDVPAHGERGIYAMLLDPKGRIQTDLRVVKAGDETLIDTEQEGAQAAKE
                     ILARYAPFSRVRLEVLSGWDVLGLYGPRATGLLGNPDLTEHEAARTEIGGASVLVVGV
                     AVPVSGYDLIGPPEALAAAREHLIETGATPAGTDAYETARIEAGVPRFGADITPQNLP
                     GESENSLERAVSFGKGCYPGQETVARMHYRGSPNKMLYRFELEPGPMEPPQAGDEILQ
                     GERKVGPISSVNVVGRLTSVAPLPVDGIIYALGYLARKADRESPMRAEDAKVLASKPA
                     "
     gene            3402..4040
                     /locus_tag="VFQ10_07935"
     CDS             3402..4040
                     /locus_tag="VFQ10_07935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9965309.1"
                     /translation="MKVVVLDVDGTLMDTNYLHTEAWARAFEEAAHRVPRAMLHKQVG
                     KGAALLISEFVEDEETIEKIQDLHSEFYGELQEYGHPLPGAKELISSLKERGYEVWFV
                     TSAKDEELEHHMQELEAEDRIDGVVNSSAVENPKPAPDIFEEALRRAGASADETVAVG
                     DAVWDVEAAREAGIRTVAVLSGGAYHKEALEEAGAVAVYEDCAALLDSDFPE"
     gene            4183..4386
                     /locus_tag="VFQ10_07940"
     CDS             4183..4386
                     /locus_tag="VFQ10_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET9965310.1"
                     /translation="MAQGTVKWFSDEKGYGFISPDDGGEDLFVHYTGIEGSGFKTLEE
                     GALVTFETSQGKKGLQAVNVTPA"
     gene            complement(4509..5687)
                     /gene="larC"
                     /locus_tag="VFQ10_07945"
     CDS             complement(4509..5687)
                     /gene="larC"
                     /locus_tag="VFQ10_07945"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HET9965311.1"
                     /translation="MIHVHFQPVGGAAGDMTLAALVAAGAPLHEIIASLRGLGVAFDL
                     AVERVDVNGIGALRADVEYHDEHSHRTFRDIRASIEVAELPERVSSRALEAFRLLAVA
                     EGAVHDEDPEEVTFHEVGAVDSIVDVIGSCLALELLDGTSVSCGPLPMGTGVVDAAHG
                     LLPVPGPATLEILEGSPVRWTDEPIETTTPTGAALMRSFTGGKFTDAAPPMTVHTTGY
                     GAGRARLRSAPNLLRAVVGELEGPEGGVELLEANVDDAPGELLGAAVERLLAAGALDA
                     WLEPIVMKRGRGAYKACALVLSGDRERFARLLMRETGTLGVRHHAVDRTVAGRRHVVV
                     DLPYGKCKVKVGSIDDEDFVVAPEYADASRLAGETGLSLLRVYEDVKAAFGRRGQHFS
                     "
     gene            complement(5760..6107)
                     /locus_tag="VFQ10_07950"
     CDS             complement(5760..6107)
                     /locus_tag="VFQ10_07950"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HET9965312.1"
                     /translation="MSEIPERIEREMFEIRSRMAPDVRDLKKHAEPQVIVKRTSTKVK
                     ERVRDAAIRFGKSLKESASRQAKMIGEAGRKRNPAPLTDAVKSDPRPLILLAISLAFT
                     LMAVRKISNGREE"
     gene            complement(6107..6544)
                     /locus_tag="VFQ10_07955"
     CDS             complement(6107..6544)
                     /locus_tag="VFQ10_07955"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET9965313.1"
                     /translation="MDGRERVAEFGRELREGPEPSDRSIKEIIDALRPQLQELVNKQI
                     ELAKTEMTPVAKRAGLASGLLAAAGVFMFVFLIILSLAAVYILDVFLSLWVSALIVSG
                     ILLLVGGILAAAGAGILRRLDPTPHRTIRTLQQNINWIKGQLR"
     gene            6778..7524
                     /gene="yqeC"
                     /locus_tag="VFQ10_07960"
     CDS             6778..7524
                     /gene="yqeC"
                     /locus_tag="VFQ10_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HET9965314.1"
                     /translation="MKLHVALGISSGDVAAFVGAGGKSGAILAVSGGLTQEGMRVLVA
                     PTTKMLSSEADMIGPLVTSEDADDLRAKTERAFSEAPAVVVGSGMISKNRVGGVDPEW
                     VGDLAGLADVVLVEADGSRRRPIKGTADHEPAFPDAATLVVAVGNVHALGTPVDEEHV
                     HRPAIFSDLTGIGQGQSITPRAFALAVAQGSLARVPQGARTAALITGVEPGRSMSDAS
                     VITRELWRLGVRNVVLASLPIESPVRVWIP"
     gene            7521..8486
                     /gene="gluQRS"
                     /locus_tag="VFQ10_07965"
     CDS             7521..8486
                     /gene="gluQRS"
                     /locus_tag="VFQ10_07965"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HET9965315.1"
                     /translation="MDSSDQPLPPRQQRGYSEKNDFDGRFAPSPTGPLHLGNLRTALL
                     AWLFARSAGARFLIRVEDLDRSRVRPGVEEAQLSDLRALGLDWDGPVLRQSERMELYV
                     EAIAGLDADGLLYPCYCTRAEIRAAASAPHSIPASDRYPGICRDLTVAQRAEREGSGR
                     PPALRVRSQGARITFRDRLLGRCEEEVDDFVVRRNDGTPAYQLAVVVDDAAQGIGEVV
                     RGADLADSTPRQILLARMLGLPAPRYAHVPLVLGPDRRRLAKRHGAVTLSERGEGPDE
                     VRAWMARSLGLARVGEMPSPDDLIARFDPERLPREPTVWSPKTAV"
     gene            8636..9826
                     /locus_tag="VFQ10_07970"
     CDS             8636..9826
                     /locus_tag="VFQ10_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET9965316.1"
                     /translation="MDVSQRFEGLQAEVEENLGEQIIALRRDIHREPELGFDTERTAQ
                     KVLAALDGLPLDIETGVARNGIVATLWGGEGPTVALRADMDALPIDEETGLPFSSEIE
                     GRMHACGHDGHTSMLVGAAHALSQRDLRDRLNGTVKFVFQPAEEGGGGGRVMVDEGVA
                     DDVASIFALHLWPGLPFGKVATKAGPIMAAADAFEMEIRGSGGHGAMPHLAADAVVIA
                     AQVVTTLQTVVSREVDPVEPAVLTVGEIGAGTAFNIIPEKARLGGTVRTLNPDLRERM
                     PGRIEALARGVAKGMRGDVNLEYTFSYPVTANEEGAADRVLSVAEDVFGAESVLELSN
                     PSMGAEDFAFFLEKVPGAFIWLGVGEDVSGLHTPKFAFDEEVLPRGSALLAALALEAG
                     QRLS"
     gene            complement(9934..10527)
                     /locus_tag="VFQ10_07975"
     CDS             complement(9934..10527)
                     /locus_tag="VFQ10_07975"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET9965317.1"
                     /translation="MFRRGSVPYGGESPEDFWGALRGVWFSPVRFFRMLDPRGGAIRP
                     AIFASVVLYLDLLLEATLQMLWLREFNYAMIYAPFLGLVVALVLAPLLVAGLAIVVLF
                     ILGGGKLSRGGFGPLFRSLGYASGIGFALWIPFAPLFAVPYGAFVATVAVKETLNVGW
                     PRAAAATLIPFGAVLLTVLALLGPDEASGFLTNPPGS"
     gene            complement(10511..11446)
                     /locus_tag="VFQ10_07980"
     CDS             complement(10511..11446)
                     /locus_tag="VFQ10_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET9965318.1"
                     /translation="MSFRSEEESGRDERAQTPTGQVPTGTDRGRPSRMSPLLLLFAGL
                     LLALLAVAAILLWRSFSESISGTSVTRDSTDAAGSEPRVRLANAAGQVRVEGVKGSRS
                     VDYEATRYAMGADPAAAKQRASEVPVDISREDSKIVIETDGGENTGADYTLRVPTGSS
                     VEVESEAGDVEVSGISGNVTVGAEAGDVTVRDVGGDVKVEAPRGDVSVGDVNTDTGSA
                     NLEAGVGDVSLEDLILGTLETSVEAGDVTLSGRFSGGGRVSVETGDIIVMLPPEDTRD
                     LTLETRVGSVLREPADGDSNGQGSETRQGSNVPQG"
     gene            complement(11468..11830)
                     /locus_tag="VFQ10_07985"
     CDS             complement(11468..11830)
                     /locus_tag="VFQ10_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HET9965319.1"
                     /translation="MDHRPPSIKVDKPWGRFEQYTHNLPSTVKIITVAPGGTLSRQYH
                     HRRDELWVILDKGAQVQLGEEVLEPETGEKIYIPRGTVHRLSTAGEREVRILEISFGE
                     FDEEDIVRLDDVYGRVVQ"
     gene            11982..12347
                     /locus_tag="VFQ10_07990"
     CDS             11982..12347
                     /locus_tag="VFQ10_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9965320.1"
                     /translation="MPKLEVEDVGTFEVPENKRLVLAIEEDAGVDILHRCGSYARCTT
                     CRIEYLEGEPEKMTRAELEVLKKRKLLGQVRLSCQTLSDHDKKVRVLLTVSSTGLDGP
                     GPHPESYITPEPEWVDKPA"
     gene            12623..13837
                     /locus_tag="VFQ10_07995"
     CDS             12623..13837
                     /locus_tag="VFQ10_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET9965321.1"
                     /translation="MRYAGPGIVIAVTGVGAGDMVSSLVAGTDFGMVLLWAVVLGAIL
                     KYFLTEGIGRWYMASGQTILQGWRSLGRWAIWYFVIYLFIVTFDFGAAVSSTAALAVD
                     AAFPGVLPLWAWAALHSVAAFVVVGIGRYGLFEMIMKSFAALKFGIVILLAILLAPSL
                     GEVALGFVPRIPEGALINVLAIIGGVGGTYSLAAYTYWVRERGWRHSAWIPTMRIDLT
                     LGYALTALFMVSMLIIGAELLFGTGTSISDEEGLLALVDPMQDRFGLLARWASLIGFW
                     AVATGAMLGAWNGGAHLFADCVRTIREVSDEEASDYLSEKSIFFRGFLAWMSFPPLIL
                     LAFGEPVLLVIFYASLGALFLPFLAITLLILLNSQRVAPEYRNRLVSNVVLAVSVIPF
                     IVVGVQEVLGSL"
     gene            14408..15814
                     /locus_tag="VFQ10_08000"
     CDS             14408..15814
                     /locus_tag="VFQ10_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HET9965322.1"
                     /translation="MLAVLVWLAAYELQGIWGRLTVIGWEGFPLQTAMVVGVFAVAVW
                     IGLRSLMGLYPGYGLSSAERLRRHTYSVVGVLAAVSFAIALDLRDITPKFEFFDLYGN
                     FPRLILALGFVGILLLSPLEQGLARWGLWRLGIWGRPVVVIGDKDTSARTVKLLEEEW
                     GLGYAPAALIDRGLTLSGKSLSSLSHEEAMTQAENLALRGGVDTIVLATPYIRRSHLA
                     PLVSRASRTFRHVLLISHLGGLTNSAVTARDFAGTLAVEIKYNLLNPWARRAKRVLDL
                     AATVVGGALVLPLLMGIALLIYAESGRPIFYTDWRMGKDGVPFPCIKFRTMVTGAEAV
                     LELMLAEDAGLREEYVKYHKLRVDPRVTHVGRFLRRTSLDELPQLWNVLRGEMSLVGP
                     RPYLPRESGEIGEARDEILRVPPGVTGPWQVSGRNHAFFTDRIEMDIHYVRDWSVWLD
                     LVLLARTVKALFVDKAAY"
     gene            complement(15842..16372)
                     /locus_tag="VFQ10_08005"
     CDS             complement(15842..16372)
                     /locus_tag="VFQ10_08005"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET9965323.1"
                     /translation="MSEQDSNPPVSTGGGDDGTTTLLGSGRIGKDDPRIVVLGDLDEA
                     SSFLGLARAEADEEVGGLLIRLQRLLYRVMGDLAMPKEDNVVGEDDLKLIDDALEEWR
                     GRTQIPKEFVIPGESRRGALLDVARSVARRAERSLVAARYANEHPDAVRVVNRLSDLL
                     FVLARNADGEKTLSKG"
     gene            complement(16484..17647)
                     /locus_tag="VFQ10_08010"
     CDS             complement(16484..17647)
                     /locus_tag="VFQ10_08010"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HET9965324.1"
                     /translation="MPPQSSSNASTTEGGSASESVLGRRALNRALLERQMLLRRRKLS
                     AFDAIERLVGMQAQVPQAPYIGLWTRLEDFEPGELSGLISGRRAVRSSMMRATIHLVT
                     DRDFLALRPAVQPVLERDVYRNATYGKEHLAGLDVDAVLAAGKALLEARPRTGAELRR
                     SLGPRWPERDAAALAYAVRGLLPLVHVPPRGLWGESGPVALTTAESWLGQSVASVRRA
                     DETILRYLAAFGPATVADARTWSGLGGLRDVFERLRPRLAIFRDEHGRELFDVPGAPL
                     PDPETPAPPRFLSQFDNALLSHAERTRIISDEHRKALFEDPMMRGVLLDGFACGTWKT
                     ERTRGKVTLVIQPFERLSKVDRDALADEGERLLRIVAKPQGAEEFEILFSRET"
     gene            17845..19392
                     /locus_tag="VFQ10_08015"
     CDS             17845..19392
                     /locus_tag="VFQ10_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9965325.1"
                     /translation="MQVRVGFPLRSEGHLGRAVALASRHPTYRVEGVNGSARVCVDLD
                     LPSDWRLLDDFSGLLRGEVDTEYAADGATLSGDELFGGLRCFLRKQRSGKTAREWCTP
                     VSLADKQLFPCRQIRVYDNDHLTGNSWYAFGKMDDEAVFEVDKDAITERVLSDLGPCV
                     RCPILNLDSTAEIVARLPERIDPSRDQSWGYREDGDLSGWKVVKTREGREEAELQEDR
                     ESLIERLARTVRSGGAGVAAASGTAPGSRNVPATRYEDVGGMRETIDLVRESVELPIT
                     HPEIFRKLHIRPHKGILFYGPPGTGKTLLARAVAHESGAHFVAVSGPEILNKFWGQSE
                     AKLRSIFNEARAKAPAIILFDEIDSFASSRDMMSESFEATLVSQLLSLMDGLNNLGRV
                     CVIATTNRPGALDPALRRPGRFDHEIEIGLPDAEARLHILGIHTREMPTDPDLDLDLI
                     AGLTGSYSGADLEALCREAALVCMRRTINLRDFEKRIASHQLSALSVTTYDFRTAMKR
                     IGPSIRR"
     gene            complement(19400..19906)
                     /locus_tag="VFQ10_08020"
     CDS             complement(19400..19906)
                     /locus_tag="VFQ10_08020"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HET9965326.1"
                     /translation="MVQVVDGRYTARMDEPFAVFIIGMRINNLFAVRKWLPTFRAMGP
                     MLKELYQHTEKGFLGGEFFLYWRGPMLVQYWRSFEDLERFARNPDDPHLPAWQRFNRT
                     VGSDGSVGIFHETYIVERGNYEAIYGNMPVFGLAKATEHVRAVGGRETARRRLMRGEN
                     EPAVPSPE"
     gene            complement(20021..20443)
                     /locus_tag="VFQ10_08025"
     CDS             complement(20021..20443)
                     /locus_tag="VFQ10_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965327.1"
                     /translation="MSPGARISAALSVLLAVGLSGVFLVAFVPSLWWIFTTYFWVAFP
                     ALGLLTSGIAGLSEGRPVRVSEEERERGLLEAIRESGEISAAAAAARTSLTVAEVDRR
                     LKELAENGHLEVRARGGAIFYALWANGEETGRRIEGST"
     gene            20640..21203
                     /locus_tag="VFQ10_08030"
     CDS             20640..21203
                     /locus_tag="VFQ10_08030"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET9965328.1"
                     /translation="MTTLGYAILGLLSREDLSGYDLTQRMAGRVGYFWSAKHSQIYPE
                     LARLEDGGYVTHSVVEQRERPDKKVYEITAEGLDALKEWVIQPPVPRPTRDELTLKTY
                     SVWLADKEQAVQLFREEELRHEEQLAHYEELRAWMEGEWQEDLTSTNSPEFASYATLR
                     RGISYEREYAAWCRWVADSIERREPSE"
     gene            21293..22471
                     /locus_tag="VFQ10_08035"
     CDS             21293..22471
                     /locus_tag="VFQ10_08035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9965329.1"
                     /translation="MMKLRRYLEGDMDALTRVGVAAFGGRISDWEQNFDPAQNARLDL
                     EQVHVIEEDGEVRASTTVLPLESFVEGEPHQMGGIAAVMVHPAYRRRGYAGELMRAVL
                     RDMRERGVSLSLLAPFAHAFYRIFGYELATEAIEYRLKPADLPISPEQSHLRAYRQED
                     LSSLMDLYEAEAKGHTLSVRRSEGHWKTSLSKKDTDVAVYERDSDVEGYILYKISGWQ
                     DRDPRRTLSVDEIVAATPRAREALLSFMAGLDPLVFGIKLYTPRGEPLHPYLRSSHVD
                     AKIEPDQMLRLVDVEGALGYLEHVPEAPLVLDISDDVIPENSGEYTVGNGKVFRGAEA
                     AESVSLDVRQLAQLYAGYLSVRSLARHGLIKASPPEALELLEALFPQGDPWLYGPDHF
                     "
     gene            22542..23543
                     /locus_tag="VFQ10_08040"
     CDS             22542..23543
                     /locus_tag="VFQ10_08040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9965330.1"
                     /translation="MKPTGTVGYAAMFEQFHPTDLLNWSKTAEESGFGSVMASDHFHP
                     WTPDQGQSAFVWSWMGALGASTNLRFGTGVTPPGFRYHPAIIAQGAATLEAMYPGRFW
                     LGLGAGEALNEHIVGDYWPEAPTRLRILMEAIEVIRRLFSGKVVKYSGEHIKLESARL
                     YTMPEIPPPIYVATAGPLQSRRTGEFCDGIITVGAPDEKIKNLMEKFEEGARRADKDP
                     SEMPRMIQLHVSWAEDQEEAEQQAVREWPNGGMPFPKADIRNPEDFAAMAKQVGVENF
                     ENRVLMSADLDEHLERVQHYVDLGFDEVYIHNVGRNQEQFIRAYGERVIPKLRWPEG"
     gene            complement(23576..24124)
                     /locus_tag="VFQ10_08045"
     CDS             complement(23576..24124)
                     /locus_tag="VFQ10_08045"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9965331.1"
                     /translation="MGRVYDDISEELALWIGKQPMFFVATAARGAHVNVSPRGLDTFR
                     VLGPKRVAWLDLTGSGVETIAHLRADGRITLMFCAFEGLPKILRLYGQGEVLESGGAG
                     YDELRSSFPDLPGERAIIDVCVGRIADSCGFGVPRMDLVGPRDNLLTSAERKGPEKMA
                     EYRALKNTRSIDGLPGLDQNCP"
BASE COUNT         4529 a         7570 c         7670 g         4682 t
ORIGIN      
        1 atggcctctt cctcggagag cccgcggctc atcaggtaga agagttggtc ctcaccgacc
       61 ttcgagacgg tggcttcgtg ctcggtgctg accttcttct cctcgatctc gatgtacgga
      121 taagtatccg ttctggcatt ctcgtcgagg agtagcgcgt cgcactcgac cctgctcttg
      181 gagttgaccg cgccctcgtg caccttcagg agtccgcggt atgtgctcct tcccgttccc
      241 ttggagatgg acttggaagt aatgaggctc gaagtgttcg gcgcggcgtg gacgaccttg
      301 ccgccagcgt cctggtgctg tccgtcgccg gcgtaggcca cggagaggat ctcgccccgc
      361 gcgccctcgc ccataaggta gaccgcaggg tacttcatgg tcaacttgga gcccaggttg
      421 ccgtcgaccc actcgatggt cgcgttctcg taggccacgc cccgcttggt gacgaggttg
      481 taggtgttgt gcgaccagtt ctggatggtc gagtacctta tcctcgcgcc aggcttggcg
      541 ataagctcca cgaccgccga atgaagcgag ttcgaagtat acgtcggggc cgtgcagccc
      601 tcgatgtagt ggacgtagga gtcctcgtcg gcgatgatga gcgtccgctc gaactggccc
      661 atgttctccg cgttgatgcg gaagtatgcc tgaagcggga tatccacgct tacgccaggc
      721 gggatgtaca cgaacgagcc gcctgaccac acggccgagt tcagcgccgc gaacttgttg
      781 tcgtccggcg ggatgatggt cccgaagtac tcctcgacga tgtcctcgtg ctcgcgcagt
      841 ccagagtcca tgtccaggaa gatgacgccc tgctcttcga gctccttctt cagggagtgg
      901 tagacgacct cggactcgta ctgcgccccg acgcctgcca ggctctccct ctcggcctgc
      961 gggatgccga gcttctcgaa ggtgttcttt atgtcgtcgg gcacatcgtc ccaggagcgg
     1021 ccctggttct ccatgggcct tatgtaatag tagatgtcgt cgtagtccat cccggagagg
     1081 tcgccgcccc aggtcggggt cggcttctcg aggaacgact ccagcgcctt cagccggaac
     1141 tctagcatcc actccggctc gttcttgtgc ttggagatca tggcgacgat ctccgggtct
     1201 atgcccttcc tcggggtcct gaagaagtac tcctccggat cgcggaaacc gtacttgtac
     1261 tcgtcgaggc cgagctcctg aatagtcttg tcttccggca ttttctcgtc ccttccctag
     1321 ctctgcgcgc ccgcgccaaa ctcctggcgc acccagtcgt agcccttctc ttccagatcg
     1381 tcagcgagct ccttgccacc cgaggtcacg agcctcccgt ccaacatcac gtggaccctg
     1441 tccggctcga tgtaccgtag tatacgctgg tagtgggtga tgatgaccgc cgagaactca
     1501 ggccccttca tctcgttgac acccttcgcc acgacctgca aggcgtcaat gtccaggccc
     1561 gagtcagtct cgtccatgat ggcgagtttg gggtcgagca tgagcatctg caggatctca
     1621 ttccgctttt tctcaccgcc ggagaagccc tcgttgaggt acctctcggc gaacttgggg
     1681 tccatgtgca tgatgctcat cttttcctgg aggagcctgt acatctccat aggattcagc
     1741 tgttcctcgc gcacggagtt gaccgcggtc ctgaggaagt tggccaccga gacgcccggt
     1801 acctcgctcg gatactggaa agcgaggaac atcccgagct tggcccgctc gtccggctcc
     1861 aactcgaaga cgtcctcacc ctggaaggtt atggagcctt cggtcacctc gtaacgcgga
     1921 tgtcccatga gcacgttggc gagcgtactc ttccccgagc cgttcgggcc catgatggcg
     1981 tgtatctcac ccttgccaac ctcgagatcc aggcccttga cgatctcctg gccgtcgatc
     2041 tctacatgaa gatcttctat cttgagcatc aaacccctgc cactcggcta tgtaaaaact
     2101 ttgctacagc agagctattt aaccacagca aattagccct aacaacgtaa agagatcacg
     2161 accaagaact tttcctgaaa cccgtgaaaa ccggctgttt acaaggattt cgtgatccag
     2221 atcacgtctt gcggaggcgt ctcgggaacc attgtcagaa ccctttgccg tagactgctt
     2281 gtaacatggt agtggacctc cgaccagggg ttattgcgaa cagttctcga taaggcgtat
     2341 gtacgaaagg atcgcagcgt attcatgccg gcttcgaggc gagcaccttc gcgtcctccg
     2401 cccgcatggg ggattcgcga tcggcctttc gggccagata gcccagcgcg tagattatcc
     2461 catctaccgg tagtggggcc accgaagtaa gacggccgac gacatttacc gaggagattg
     2521 ggcccacctt cctttcgccc tgcaggatct catctcccgc ctgaggcggt tccatcgggc
     2581 cgggctccag ctcgaagcgg tagagcatct tgttcgggct gccacggtaa tgcatcctcg
     2641 ccaccgtctc ctggcccggg tagcaaccct tcccgaagct gacggcccgc tccagggagt
     2701 tctccgactc gccagggaga ttctggggtg tgatgtcagc gccgaagcgg gggacgccgg
     2761 cttcgatgcg ggcggtctcg taggcgtcgg tccctgcggg ggtcgcgccg gtttcgatca
     2821 ggtgctcgcg ggccgcggcg agggcttctg ggggcccgat caggtcgtag ccgctgacgg
     2881 gcaccgcgac gcccacgacg agaaccgaag cgccgccgat ctctgtccgt gctgcctcgt
     2941 gttcggtgag gtcagggttt ccgagtagcc ctgtggcccg aggtccgtag aggccaagca
     3001 catcccagcc ggagagcacc tccagtctca cgcgggagaa gggagcgtag cgggccagga
     3061 tctccttcgc cgcctgagcg ccttcctgtt ccgtgtcgat gagggtctcg tcgccggctt
     3121 tgacgacacg gaggtccgtc tggatccggc ccttcggatc gaggagcatg gcgtagatac
     3181 cgcgctctcc gtgcgccggc acgtcgttgg tcagaacggc gttcagcatt ccgatgggat
     3241 ccttgcccgt gagacgcagg agccttctat ccgagcggtc cacgacggcg gcgccttctc
     3301 tgtgggctgc gtagccttcg aggtcgtttg ggtcggcgtt gttttgggga cctttcaaat
     3361 acttcatggg tctattctaa ggcacgagag gagtctggat ggtgaaagtc gtcgttctgg
     3421 acgttgacgg cacgttgatg gacaccaact atttgcacac ggaggcgtgg gcgcgggcgt
     3481 ttgaggaggc ggcccatcgg gtgccgaggg ccatgcttca caagcaggtc gggaagggcg
     3541 cagcgctgtt gatcagcgag ttcgtggaag acgaggagac aatagagaag atccaggacc
     3601 tccacagcga gttctacggg gagctgcaag agtacggcca tccgctcccg ggagcgaagg
     3661 agttgatctc ctcgctcaaa gagcggggct acgaggtctg gttcgtcacc agcgccaaag
     3721 acgaagagct ggaacatcac atgcaagaac tcgaagccga ggatcgcata gacggcgtcg
     3781 tcaactcgag tgccgtcgag aacccgaagc cggcccccga catcttcgag gaggccctgc
     3841 gaagagccgg ggcgagcgct gacgagaccg tcgccgtcgg tgatgcggta tgggacgtcg
     3901 aggctgctag ggaagccggc atacgtaccg ttgccgtgct ctccggcggc gcctaccaca
     3961 aggaggctct cgaagaggcc ggagcggtgg ccgtctacga agattgcgcc gcgctccttg
     4021 actctgactt ccctgagtag cgccgggtgc gcgtgaaagc ctggggtttt tgtactagag
     4081 tgccacacgc aagctggaag agggtctggt ctgtggaggg catacccgca ggtctgtcat
     4141 ccaaagatcg ggctcaggaa gagaaaggtg ggcgcaaagg gtatggccca gggaacggta
     4201 aagtggttca gcgacgagaa gggctacggc ttcatctccc cagacgacgg gggtgaggac
     4261 cttttcgtcc actacacggg gatagagggt agcgggttca agaccctcga agaaggcgcc
     4321 ctcgtgacct tcgagacatc ccagggaaag aagggactgc aggcggtaaa cgtaacacca
     4381 gcctgacagc aagcgcctgc cagagcgagg ccaccacacg cagagccagg ctccgtggca
     4441 aacgatgcgg tgccttgtac accgcataga gagcacgacc agctcgagta gctccccaac
     4501 gatgcttact agctgaaatg ctgaccgcgg cggccgaagg cggccttcac atcctcgtac
     4561 accctgagta gcgagagccc ggtctccccg gcgagcctgg aggcgtcggc gtactcggga
     4621 gcaacgacga agtcttcatc gtcgatgctt cctaccttga ccttacactt cccgtaggga
     4681 aggtctacca cgacgtggcg gcgccctgcg acggtgcggt cgaccgcgtg gtggcgcacg
     4741 ccgagggttc cggtctcccg catgaggagc ctggcgaagc gctcgcggtc gccggagagg
     4801 acaagggcgc aggccttgta agcgccgcgg ccacgcttca tcacgatggg ttcgagccag
     4861 gcgtcgaggg cgccggccgc gaggagacgc tctaccgcag ctccgagaag ctcgcctggc
     4921 gcgtcatcga cgttggcctc cagtaactcg acgccacctt caggcccttc gagctccccg
     4981 acgacggcgc gcagcaggtt gggcgcgctc ctcaggcgcg cccttccggc cccgtagccc
     5041 gtggtgtgga cggtcatggg cggggccgcg tccgtaaact tgccaccggt gaacgatctc
     5101 ataagcgcgg cccccgttgg ggtggtcgtc tcgatgggct catcggtcca gcggacaggc
     5161 gagccctcga ggatctccag cgtcgccggt ccaggcaccg ggaggagacc gtgggcggca
     5221 tcgacgacac ccgtgcccat gggcagagga ccgcagctta cggaggtccc atcgagcagc
     5281 tccagtgcca ggcagctgcc gatcacgtcg actatcgagt ccacagcccc gacctcgtgg
     5341 aaggtgactt cctccgggtc ctcgtcgtgg acagcgcctt ccgcgacagc cagaaggcgg
     5401 aaggcctcca gcgcgcgtga ggagactctc tccggcaatt cggcgacctc tatagaggcg
     5461 cggatatccc tgaacgtcct gtgggagtgt tcgtcatggt attcgacgtc tgccctgagt
     5521 gcgccgatgc cgttcacatc gacacgctcc acagcgaggt cgaaggctac accgaggccg
     5581 cgcagggagg ctatgatctc atgcagcggc gcccccgccg cgacgagcgc ggccagggtc
     5641 atgtcgccag ccgcgccacc aacgggttgg aagtgaacgt ggatcaagac ccgtcccttg
     5701 actttgtggt gaccggcctc aggtgggtgg gggtattcgg ggctagggga accgcccctc
     5761 tattcctctc gtccgttgga gatcttgcgc acggccatca gagtaaaggc caggctgatg
     5821 gcaagtagaa tcagtggcct cgggtcgctc ttcacggcgt ccgtgagggg tgccgggttc
     5881 ctcttcctgc ccgcctcccc gatcatcttc gcctggcgac tggccgattc cttgaggctc
     5941 ttgccgaagc ggatcgcggc gtccctgacg cgttctttga ccttcgtaga ggtccgcttc
     6001 acgataacct ggggctcggc gtgctttttg agatccctga cgtcaggcgc catgcggctc
     6061 cggatctcga acatctccct ttctatgcgc tcgggtatct cgctcattac ctcaactgcc
     6121 cctttatcca gttgatgttc tgctgaaggg tacggatcgt ccggtggggc gtgggatcca
     6181 gcctcctcag tatgccggcg ccggccgcgg cgagtatccc accgaccaac agcagtatac
     6241 cactgacgat cagagccgag acccaaagtg agaggaagac atccagtatg taaaccgcgg
     6301 caagggagag gattatgagg aatacgaaca tgaagacgcc ggcagccgcg agaaggcccg
     6361 aagccaaccc ggccctcttg gctacgggcg tcatctccgt cttcgccaac tcgatctgct
     6421 tgttgacaag ctcctgaagc tggggcctca gagcgtcgat gatctccttg atgctccggt
     6481 cggaaggctc gggaccctcc ctgagctccc tcccgaactc cgctacccgc tctcgcccat
     6541 ccatccccac gctcctttct tctgatcctg agacttcgag ttcttcttgc ctttcacccg
     6601 ctcttagtta cccttgccat gccctatgta atccgctagg tctcctggaa atagccatcc
     6661 ttatcctaca ccacatctta gtatccaaca tgtcataaag cacgcgaagt gaatcgatga
     6721 tgtaacatga atacaggtgt acaagacccg tcgcctgctg gcgggatggg agtggcattg
     6781 aagttgcacg tggcgttagg gatctcctcg ggggacgtgg ccgccttcgt cggggcgggg
     6841 ggcaagtccg gtgcgatcct tgctgtctca ggcgggctca cgcaggaggg tatgagggtc
     6901 ctcgtggccc cgacgacgaa gatgttgtcg agcgaggccg acatgatagg cccgctcgtc
     6961 acctcagaag acgccgatga cctgcgcgca aagaccgaga gagccttctc cgaggctcct
     7021 gcggtggtcg tgggatcggg gatgatctcc aagaacaggg taggaggcgt cgaccctgaa
     7081 tgggtaggcg acctcgccgg gcttgccgac gtggtcctgg tcgaggccga tggatcgcgg
     7141 cggcgcccga tcaaaggtac cgcagatcac gagccagcat tccctgatgc ggccaccctg
     7201 gtcgtcgccg tcggcaacgt ccacgccctt ggcactcccg tggacgaaga acacgtccac
     7261 cgccccgcca tcttctccga cctcacaggc atcggtcagg gccagagcat aaccccgaga
     7321 gccttcgcct tggccgtggc acaaggctcc ctagccagag tcccgcaagg ggccaggact
     7381 gccgccctga taaccggcgt cgaaccgggc cgcagtatgt ccgacgcctc cgtcatcacc
     7441 cgcgagctct ggcgcctcgg cgtcaggaac gtcgtcctcg cctctctccc catcgaatca
     7501 ccggtccgag tctggatccc atagactcct ccgaccagcc gctcccaccc cgacagcagc
     7561 gagggtacag cgagaagaac gacttcgatg gacgcttcgc gccaagcccc actggccccc
     7621 ttcacctcgg gaaccttcga accgctctcc tggcgtggct gttcgcgcgt tcggcagggg
     7681 cgcggtttct gatacgcgtg gaggacctcg accgctcgcg ggtacgtcct ggcgtggagg
     7741 aggcgcagct ctctgacctc agggctctcg gactcgattg ggatggaccc gtcttgcgcc
     7801 agtcggagcg gatggaactg tatgtggagg ccatagccgg gctcgatgct gacgggctgc
     7861 tctacccttg ttactgtacg cgggcggaga tccgggccgc cgcatccgcg ccgcacagca
     7921 tccctgcttc agaccgttat cccggcatct gccgtgacct aacggtggcg cagcgcgccg
     7981 agcgcgaggg ctcaggacgc cctccggcgc tgcgggtgcg ttcgcaggga gccaggataa
     8041 ccttcaggga ccgacttctc ggacgctgtg aggaagaggt ggacgacttc gtcgtgcgcc
     8101 gcaacgacgg cactccggcc taccagctcg ccgttgtggt cgatgatgcc gcgcagggga
     8161 taggcgaggt ggtgaggggt gcggatcttg cggactcgac cccgcgccag atcctgctag
     8221 cacgaatgct cggcttgcca gcgccccgat acgcccacgt cccgctcgtg ctcggcccgg
     8281 acagacggcg tctcgccaaa cgccacggcg ccgtcacgct ctcggagcgg ggtgaggggc
     8341 ctgacgaagt acgcgcctgg atggcccgct cactcggcct cgccagagtc ggggagatgc
     8401 catcaccgga cgacctcatc gcccgcttcg acccggaacg cctgccgcgc gagccgaccg
     8461 tctggtcacc aaagaccgca gtctgagccg cgctctatgg tacctttcct aagcagcgcc
     8521 gcgacggtgc cggcccgtca gcaaaccaaa gtgctgacaa gctgaagtgc tgactagctg
     8581 accagcgcgg gtttggattg gtgaaccctt ccaaaccgct atgagaggag actttgtgga
     8641 cgtatcccag aggttcgaag gattgcaagc agaggtcgag gagaacctcg gtgagcagat
     8701 catcgccctc cgccgcgata tccaccgcga gcccgagctg ggcttcgata ctgagaggac
     8761 cgcacaaaag gtgctagcgg cactcgacgg tctacccctc gacattgaga ccggcgtcgc
     8821 gcggaacggg atcgtcgcca cgctctgggg aggagaagga cctacggtag cgttgcgggc
     8881 agacatggat gccctcccca tagatgagga gacgggcctg cctttctcct cggagataga
     8941 aggcaggatg catgcctgtg gccacgacgg acacaccagc atgctcgtcg gcgcggccca
     9001 cgccctctcg cagcgagacc tgcgcgaccg tttgaacggc accgtcaagt ttgtgttcca
     9061 gcctgcggag gagggtgggg gtggaggcag agtcatggtc gatgaggggg tggcagacga
     9121 tgtcgcttct atcttcgcgc tgcatctgtg gccggggttg cctttcggga aggtggcgac
     9181 gaaggcgggt cccatcatgg ccgctgccga cgctttcgag atggagatca gaggctcggg
     9241 cggtcacggc gcgatgcccc acctggccgc cgacgccgtg gtcatcgctg cacaagtagt
     9301 gacgacgttg cagaccgtcg tgtcgaggga ggtggacccg gtggagcccg cggtcttgac
     9361 ggtgggggag atcggcgccg gtacagcctt caacatcatc ccggagaagg cccgcctcgg
     9421 gggaacggtc aggaccctga accctgacct ccgcgagagg atgccggggc gtatagaagc
     9481 cttggcccgc ggtgtggcga aaggtatgcg tggcgacgtg aatctggagt acactttctc
     9541 ttaccccgta accgccaacg aggaaggtgc tgcggaccgc gtccttagcg tagccgagga
     9601 cgtattcggt gcggaatccg tcctggagct ctccaaccca tcgatgggcg ctgaggactt
     9661 cgcgttcttc ctggagaagg tgccaggggc ctttatctgg ctcggcgtcg gggaagatgt
     9721 ctcggggttg cacacgccca agttcgcctt cgatgaggag gtcctgccgc gaggatcggc
     9781 gctgctggcg gcgctcgcgt tggaagcagg tcagcgtctc agctagtcag cacgacgccc
     9841 tgcgggcggc ttgtcaacca gggttcgcac gcgaacgctg aatagttgct aggtgaaagc
     9901 gccccacagg gcgcgtgctg agatgctaga gcattagctg ccaggtgggt ttgtaaggaa
     9961 gcctgaggct tcatcagggc cgagcagggc gagcacagtc aggaggacgg ccccgaatgg
    10021 gatgagggtc gccgcggcgg cccgaggcca accaacgttc agagtctcct ttacggccac
    10081 ggtggccacg aaggcgccgt atgggacagc gaagaggggg gcgaagggga tccagagcgc
    10141 gaaacctatc ccgctcgcgt agcctagaga gcggaacaga ggcccaaagc ctccgcgcga
    10201 gagcttgcca cctccgagga tgaacagaac gacgatcgcc agccctgcga cgagcaacgg
    10261 tgcgagcaca agcgcgacaa cgagccccag gaagggagcg tagatcatgg cgtagttgaa
    10321 ctcgcggagc cagagcatct ggagtgttgc ctcgaggagc aggtccaggt agaggaccac
    10381 ggaggcgaag atggccggcc ggatcgctcc tccacgcggg tcgagcatcc tgaagaagcg
    10441 gacaggagag aaccagaccc ctcgcagcgc gccccagaag tcttcgggcg attcaccacc
    10501 gtatggcacg ctacccctgc ggaacattag acccttgccg cgtttcggaa ccctgcccgt
    10561 tcgaatctcc gtccgcaggc tcgcgcaaga cgctgccgac gcgtgtctcg agcgtaaggt
    10621 ccctcgtatc ctccggagga agcataacga taatgtcgcc agtctctacg gagactcgtc
    10681 cgccgccgga gaaacgccct gaaagggtaa cgtccccagc ctcgacgctg gtttcgaggg
    10741 tgccaaggat caagtcttcg agcgagacgt cccccacgcc ggcctcgaga ttcgcactcc
    10801 cggtatccgt gttgacgtcg ccgacgctga catctcctct tggggcttcg accttgacgt
    10861 cgccaccaac gtctctgacc gtcacgtccc ctgcttcggc gccgaccgtc acgttgcccg
    10921 agataccaga gacctcgacg tctccagcct cggattcgac ctcgaccgag ctgcctgtcg
    10981 gtaccctgag cgtatagtcg gcacccgtat tctctcctcc gtccgtctcg atgacgatct
    11041 tcgaatcctc gcgggagatg tccactggaa cttcggaggc ccgctgcttg gcggcggcgg
    11101 ggtcggcgcc catcgcgtat cgcgtggcct cgtaatctac ggacctggat ccctttacgc
    11161 cctcgacgcg cacctggcct gcggcgttgg cgagtcttac gcgcggttca gaaccggctg
    11221 catccgtgga atccctggtg acgctggtgc ccgagatgct ctcgctgaag gacctccaga
    11281 ggaggatcgc ggccactgcg agcagcgcaa ggagcagccc ggcgaacaac aggagcagcg
    11341 gcgacatgcg ggaaggccgc ccgcggtccg tccctgttgg tacctgaccg gtgggcgtct
    11401 gtgcgcgctc gtcgcgcccg gactcctctt cgctcctgaa gctcaccctg gatctagacc
    11461 agaaccgtta ttgcaccacg cgaccgtaga cgtcatcgag cctgacgatg tcctcctcat
    11521 cgaactcgcc gaaggagatc tccagtatcc gcacctccct ttcgccggcc gtggagagcc
    11581 tgtgaaccgt tcctcggggg atatatatct tctccccggt ctcgggctcg agtacctctt
    11641 cgccgagctg tacctgggct cccttgtcca gtatgaccca gagctcgtcg cggcggtggt
    11701 ggtactggcg cgagagcgtg ccgccgggag cgacggtgat gatcttgacc gtactgggta
    11761 ggttgtgcgt gtactgttcg aaccggcccc agggcttgtc tacctttata gaaggcgggc
    11821 ggtgatccat gcgtgacccc tttcgtgaat ggccagttta gccgatccac ttacggtttt
    11881 ggagacgtat aaccccgaaa gtccgcgacc tggcattcgc gcctggtctg ccggcgtggt
    11941 tagatacaat aacgaagctc cgtctctgga aggagggata aatgcccaag ctagaggtcg
    12001 aggacgtggg gaccttcgaa gttcccgaga acaagagatt ggttttggcg atcgaagagg
    12061 atgctggggt ggatatcctg caccgctgtg gttcttacgc caggtgcacc acatgccgca
    12121 tcgagtatct ggagggtgag ccagagaaga tgacaagggc cgaactcgag gtgctgaaga
    12181 agcgcaaact cctcgggcag gtcaggctct cgtgccagac tctaagcgac catgacaaga
    12241 aggtgcgggt attgctgacc gtgagctcca ccggcctcga cggtcctggt ccgcatcccg
    12301 agtcctatat cacacccgaa cccgagtggg tggataagcc ggcctgaggc acagctttcc
    12361 cggagacctc gagcaggggc ctcaagccgc tcaccacatt cgcagatgaa gcgctgagac
    12421 accaagctcg tggtcagcag aaagacgaat attcacgggg ttcggtgcgg agcgtcctag
    12481 ctctgtctct cctttgcttg taacgcaacg atggtgtcta atacggtgta ttcgttggcg
    12541 agcgtgggtg aggggacaga tggttgacgt taccgatgaa ggtgcttcga ggacgcaggt
    12601 tccatccact ctaggacgcc ggttgaggta cgcgggaccc ggaatagtca tcgcggtgac
    12661 cggcgtgggg gccggggaca tggtctcctc cctggtagcc gggacggact ttggaatggt
    12721 cctgctgtgg gctgtcgttc tcggcgcaat cctcaagtac ttcctgaccg agggaatagg
    12781 ccgttggtac atggcctctg gacagaccat cttgcagggc tggcgttcgc ttggccgttg
    12841 ggcgatctgg tacttcgtga tctatctgtt catcgtgacc ttcgacttcg gggccgcggt
    12901 atcctcgacc gctgcgctgg ctgtggacgc ggcgttcccc ggagtgctgc cgctgtgggc
    12961 ctgggccgcc ctccacagcg tggcggcgtt cgtcgtcgtc ggcataggcc gctacggatt
    13021 attcgagatg atcatgaagt ccttcgccgc cctgaagttc gggatcgtca tactgctggc
    13081 tatcctgctc gccccgtccc ttggcgaagt cgccctcggg ttcgtcccgc ggataccgga
    13141 gggcgccctg atcaacgtcc ttgccattat cggcggcgtg ggcgggacct actcgttggc
    13201 cgcctacact tactgggtgc gcgagcgcgg ttggcggcat tcggcctgga tcccgaccat
    13261 gcggattgac ctgactttgg gctacgcgct aaccgccctg ttcatggtgt cgatgctgat
    13321 catcggcgcg gagttgctct tcggtacagg cacgtctata agcgatgagg agggcctcct
    13381 ggcgctggta gaccccatgc aggacagatt cggcctcctg gcacgatggg cctccctcat
    13441 cggcttctgg gccgtcgcga ccggagccat gctcggagcc tggaacggcg gcgcccacct
    13501 ctttgctgac tgtgtgcgca ctatccgcga ggtgtctgac gaggaggcat ccgattacct
    13561 ctccgaaaag agcatcttct tccgaggatt tctagcctgg atgtcgttcc cgcccctgat
    13621 cctgctggcc ttcggagagc cggtcctgct ggtgattttc tacgcctcgc tcggtgctct
    13681 tttcctgccc tttttggcga ttacgctgct tatactcctg aactcccagc gtgtggcgcc
    13741 cgagtaccgc aacaggctcg tctccaacgt ggtcctcgcg gtctcggtga ttccctttat
    13801 cgtggtcgga gtgcaggagg tgctaggtag cctgtaaccg cagggccatt cgtaacagaa
    13861 agtgccccac tcgcaacagc taaaatcatt catttgagta gttaataggt gtatccgttc
    13921 agtttatgtt gcgttatagt aaggtcaggg tgctgcgtcg acagacgaga agcgtatata
    13981 ccaaggaacg atgggaggct gctgaatcac tctgagaact gcggagtatg aaaggtgccg
    14041 gcctttgggc cgaggatcta cagctgtaag ggcttactga tgtagtagct tcgccaaggt
    14101 caggaggggg aatggtgaga aggacggccg aacttctcaa gcgaagtatg tctgtcttga
    14161 aagcgatgat ggctacgttg attctctgga caattcgcga tgtggctcag tatacgacgc
    14221 cggaaaagta ccatcgtact cggttgaggg ggagatggtc ttgaggagga gtcaagtccc
    14281 cagtatcggt aatacggctc gacaggcaga agagccccca tcgcagcacg cgcggccgcc
    14341 aaggcgccgc ctggcgcagg tttggagaca accaggggtt gtcctggtct tgttattgtc
    14401 ggacgccttg ctggcggttc tggtctggct ggcggcgtac gagctacagg gcatctgggg
    14461 ccggttgacc gtgatagggt gggaaggctt ccctctgcag acggctatgg tggttggggt
    14521 tttcgccgtg gcggtgtgga tagggttacg ctctttgatg ggactctatc cggggtacgg
    14581 tctgagctca gcggagaggc ttcgccggca cacatactcc gtggtcggcg tcctcgccgc
    14641 cgtttccttt gccatagcat tagacctcag ggacataact ccaaagtttg agttcttcga
    14701 cctttacggg aacttcccac gcctgatatt ggcgctaggc tttgtgggca ttttgcttct
    14761 atcccccctg gagcagggtt tggcgcggtg ggggttgtgg agactcggca tttggggcag
    14821 acccgtggtc gtcataggag ataaggacac gagcgcccga acggtcaagc tcttggaaga
    14881 ggaatggggg ctgggatacg cccccgccgc ccttatcgat cggggcctga ctctgtcggg
    14941 gaagtcgctc agcagcctct cccacgaaga ggccatgacc caggccgaga acctcgctct
    15001 cagaggcggc gtggacacca tagtcctcgc cacgccttac atccggcgct cgcacctggc
    15061 accgctcgtt agccgggcta gccgcacctt ccggcacgtt ctgcttatct cgcacctcgg
    15121 cggactgacc aactcggcag tgacggcccg cgactttgcg ggaaccttgg cggtggagat
    15181 aaagtacaac ctcctcaacc cctgggcgcg tagggcgaaa cgcgtgctgg accttgcagc
    15241 taccgtggtg ggcggagccc tcgtgctacc gcttctcatg gggatagcct tgctcattta
    15301 tgcagagtct ggaaggccca ttttctacac ggactggcgg atgggcaagg acggtgtccc
    15361 attcccgtgc atcaaattcc gtactatggt aaccggagcg gaggctgtgc tggagcttat
    15421 gctcgccgag gatgctggcc tcagggaaga gtacgtaaaa taccacaagc tacgcgtcga
    15481 tccccgtgtt acgcacgtcg gccggttctt gcgcaggacc agcctggatg agctaccgca
    15541 gctttggaac gtcttgcgcg gggagatgag cctggtcggc cctcgcccct acctgccccg
    15601 cgagtccgga gagatcgggg aggcgcgcga cgagattctg cgcgtacctc caggtgtaac
    15661 cggaccgtgg caggtttctg gtcgcaacca cgctttcttc actgacagga tcgagatgga
    15721 tatccactac gtacgcgact ggtccgtatg gcttgatctg gtgcttctgg cccgcaccgt
    15781 taaagccctc tttgtcgaca aagcagctta ttagtcgccg tcaatggtga ctaagtaccc
    15841 tctagccctt cgatagggtc ttctccccgt cggcgttgcg cgcgaggacg aagagtaggt
    15901 cggagaggcg gttgacgacg cgcacggcgt cggggtgctc gtttgcgtat cttgcggcga
    15961 cgaggctacg ctccgcgcgg cgcgctacgg agcgggcgac gtcgaggagc gcgccgcgcc
    16021 ggctctcacc agggatgacg aattccttgg ggatctgtgt gcgtccgcgc cactcttcga
    16081 gggcgtcgtc gatgagcttc aggtcatcct cgccgaccac attgtcctcc ttcggcatcg
    16141 ccaagtcgcc catgacgcgg tacaggagcc gctgcagccg gatcaagagt ccaccgacct
    16201 cctcgtccgc ctcggctcgg gccaggccga ggaaagagct cgcctcgtcc aagtcgccga
    16261 gcacgactat ccgtgggtcg tccttgccaa tccgtcccga cccgagcagt gtggttgtcc
    16321 cgtcgtcgcc cccgcctgtc gagaccggcg ggttgctatc ttgttcactc aaagggtctc
    16381 ctgttctctt cggggtgcac cggcaaggta gcccgcccga tcgcgcagcg caagccgagg
    16441 gaattacaga gctatgaacg taggtgactc acccctgtac tttctaggtt tccctgctga
    16501 acaggatctc gaactcctcg gcgccctgag gtttcgccac aatgcgcaac agccgctcgc
    16561 cctcatcggc cagggcgtcg cgatccactt tggagagtcg ttcgaacggc tggatcacga
    16621 gcgttacctt tccgcgggta cgctccgtct tccacgtccc gcaggcgaat ccgtcgagca
    16681 gcacgccgcg catcatgggg tcctcgaaga gagctttgcg gtgctcatct gagatgatgc
    16741 gggtacgctc ggcgtgggag agcagcgcat tgtcgaactg ggacaagaag cgaggaggtg
    16801 caggtgtctc gggatcggga agcggggcgc cgggcacgtc gaagagctcg cgaccgtgct
    16861 cgtcacggaa gatagcaagg cgcggccgga gccgctcgaa gacgtcccgc aacccgccaa
    16921 ggcccgacca cgtcctggcg tcagcgacgg tggccgggcc gaaagccgcc aggtagcgta
    16981 agatagtctc gtccgccctg cggacgctgg ccacgctttg gccgagccat gactcggccg
    17041 tggtcaaggc tacgggtccg ctttcgcccc agaggcccct cggaggaacg tgcaccagcg
    17101 gcagcaggcc gcgtaccgcg taagccagcg ccgccgcatc tctctccggc catcgcggcc
    17161 cgagtgaccg gcgcagctcc gcgccggtcc gtggtctcgc ttctagcagc gccttaccgg
    17221 ccgccagcac cgcgtcaacg tccagtcctg cgaggtgttc cttgccgtag gtcgcgttac
    17281 ggtatacgtc acgttccagg accggctgca cagcagggcg caaagccagg aagtcgcggt
    17341 cggtgacgag gtggatcgtc gcgcgcatca ttgaggagcg caccgcccgc cggccggaga
    17401 tcaagccgga taactcgccg ggttcgaagt cctcgagccg cgtccagagg ccgatgtagg
    17461 gagcctgggg cacctgggcc tgcatgccga caagccgctc gatggcgtcg aaggcggaga
    17521 gcttccggcg gcgcagcagc atctggcgct ccaacagcgc gcggttgagc gcgcgccgac
    17581 cgagcacgct ctccgaggcg ctaccgccct ctgtggtcga agcgttggat gacgattgtg
    17641 ggggcatcct tgtcctttcg cgccggttgg ccgcgccata gcttctgctc cctgttacgg
    17701 tgagatggta cccgcgaaag cggtcacaag cgttccgtat tggcctggat ctccaaaaga
    17761 agtcgaagaa ttacgattct tacgccagat tcctgacatc agtcaccgac ggttccggat
    17821 agcgtgggtt agaatctacg ggccatgcaa gtgagagttg ggttcccgtt gagatccgaa
    17881 ggccatctcg gccgggccgt agcccttgcg tcccgccatc ccacctaccg cgtagagggc
    17941 gtgaacggct cggcacgggt ctgtgtagat ctagatcttc cttccgactg gcggctcctc
    18001 gacgacttct cagggctcct gcgcggcgag gtcgacaccg agtacgcggc cgacggagcc
    18061 acgctctccg gggacgagct cttcgggggc ttgcggtgtt tcctgcgcaa gcaacgctcc
    18121 gggaagacag cgagggagtg gtgcacgccg gtttccctcg cggacaagca gctcttccct
    18181 tgccgtcaga tccgggtcta cgacaacgac catctcacag gcaactcatg gtacgccttc
    18241 ggcaaaatgg acgatgaggc cgtcttcgag gtggacaagg acgccatcac cgagcgtgtc
    18301 ctttcggacc tcggaccgtg cgtcaggtgc cccattctga acctcgactc gaccgccgag
    18361 atagtggccc gcctccctga gaggatagac cctagcaggg accagtcctg gggttacaga
    18421 gaagacggag acctctcggg ctggaaggtc gtgaagacgc gggaagggcg cgaagaggct
    18481 gagttgcagg aggataggga gagtcttatc gaacgcctgg cgcggactgt gcggagtggg
    18541 ggagcgggtg tcgctgcggc gagcggcaca gcgccgggca gtcgcaacgt gccggctacc
    18601 cgctacgagg atgtcggcgg gatgcgcgag actatagacc tggtccgcga gtcggtggaa
    18661 ctgcccataa cccaccctga gatctttcgt aagctccaca tcaggccgca caaaggcatc
    18721 ctcttctacg gaccgccggg gacgggcaag acactcctgg cgagggccgt cgcccacgag
    18781 agcggggccc acttcgtggc cgtctctggg ccggagatcc tcaacaagtt ctggggccag
    18841 agcgaggcga agttgcgctc catcttcaac gaagcccgcg ccaaagcgcc ggcgatcatc
    18901 ctcttcgatg agatagattc cttcgcctca tcgcgtgaca tgatgagcga gagcttcgag
    18961 gctacgctgg tctcacaact cctatccctc atggacgggc tcaacaatct gggcagagtc
    19021 tgcgtcatag ccaccaccaa tagaccaggt gcgctcgacc cagccctcag gcgtcctggc
    19081 cgcttcgacc acgagatcga gatcggactt cccgacgccg aagcgcggct tcacatactc
    19141 gggatccaca ctcgcgagat gcccacggac cctgacctcg acctggatct gatagcgggc
    19201 ctcaccggaa gctactcggg cgccgacctg gaagccctct gccgcgaggc cgcccttgtt
    19261 tgcatgcgtc gcaccataaa cctccgcgat ttcgagaagc ggatagcctc ccaccagctc
    19321 tcggccctct ccgtcaccac ctacgatttc cgcaccgcca tgaaacgcat aggccccagt
    19381 atccggcgct agagctgtac tactcaggcg aagggacggc cggttcgttc tccccgcgca
    19441 tcagacggcg cctcgctgtc tccctccctc cgaccgccct gacgtgctcg gttgcctttg
    19501 ccaatccgaa gacaggcatg ttgccgtaga tcgcctcgta gttcccgcgc tccacaatgt
    19561 atgtctcgtg gaagatgccc acgctgccgt ccgagcctac cgtccggttg aacctttgcc
    19621 atgcgggcag gtgcgggtcg tccgggttcc tggcgaagcg ctccaggtcc tcgaaggagc
    19681 gccagtactg gacgagcata ggcccgcgcc agtaaagaaa gaactcgcca ccgaggaagc
    19741 ccttctcggt gtgctggtag agttccttga gcatcgggcc catcgcgcgg aaggtcggga
    19801 gccacttcct tactgcgaag aggttattta tcctcatccc gataatgaaa accgcgaacg
    19861 gctcgtccat ccgcgccgtg tacctaccgt ctactacctg caccatccta ctctcctgtc
    19921 cgagtccaaa aggttctgat ccacagcgct ctaccccgtc accagcgccg cgaactatcc
    19981 cgggccgtcc gcgagcctat cgccctatct acgcccgagc tcacgtcgaa ccctcgatcc
    20041 gccgcccggt ctcctccccg tttgcccaca gcgcgtagaa gatggcgccg cccctggccc
    20101 tgacctcgag gtggccgttc tcggctagct ctttgagcct cctgtcgacc tcggcgaccg
    20161 tcaacgaggt cctcgctgcg gccgctgcgg ccgagatctc accgctctcc cgaatcgcct
    20221 ccagcaaccc ccgctcccgc tcctcctcgg aaacccggac aggtctacct tcactaaggc
    20281 ccgctatacc acttgtgagc agccctaacg cggggaaagc gacccagaag taagtcgtga
    20341 agatccacca caaagaaggc acgaaagcta ccagaaacac cccagagagc cccaccgcta
    20401 agagcaccga gagcgcagcc gagatcctcg ccccaggact cattttatcc acctcgcgcc
    20461 ccaaaaactc acgccctcca ccctgccgcc acgcaccatg cctcattcca cgccccacgt
    20521 aagacccacc gcacatctct cgctcctctc tcatgtcctt tatgtaaacg ctgacatatg
    20581 gtactctaag ggccggtgga cgatatgtca agagtgacat aacaaaatat tggaggagta
    20641 tgacgacgct agggtatgcg atccttggtt tgctatcgag ggaggatctc agcgggtacg
    20701 atctgacgca acggatggct ggacgggtcg gatatttctg gagtgcgaag cacagccaga
    20761 tctaccctga gttagcgagg ctggaggatg gcggctacgt gacgcacagt gtggtcgagc
    20821 aaagagagag gcctgacaag aaggtgtacg agatcacggc cgaggggttg gatgcgctga
    20881 aagagtgggt gatccagcct cccgtgccaa ggccgacgcg ggacgagttg acgttgaaga
    20941 cgtactcggt ctggctggcg gacaaagagc aggccgtgca gctcttccgt gaagaggaac
    21001 tcaggcacga ggagcaactc gctcactacg aggagctacg ggcctggatg gagggagagt
    21061 ggcaagaaga tctgacctct accaatagcc ccgagttcgc ctcctacgct acgctccggc
    21121 gcggcatcag ctacgagaga gaatatgctg cgtggtgccg gtgggtggcc gattctatcg
    21181 agagacgcga accttcggag tagcgagaga aaggcgggag cgaggagaac ggtgggtgat
    21241 ccctgcctcg ctcttccggt agaatcctgg cgcaccctag aagagccgga ggatgatgaa
    21301 gctacgcagg taccttgaag gggacatgga cgcgctcacg cgtgtcgggg tcgcggcctt
    21361 cgggggcagg atctccgact gggagcagaa cttcgacccg gcccagaatg cccgtctcga
    21421 cctcgagcag gtccatgtca tcgaggagga cggggaggtc agggcgagca ccaccgtatt
    21481 gccgctggag tcctttgtgg agggagagcc gcatcagatg ggcggcatcg ctgcggttat
    21541 ggtccatccc gcctacaggc ggcgaggcta cgcgggggag ttgatgcgcg ccgtgctacg
    21601 cgacatgcgt gaacggggcg tctcgctatc gctgctggcc ccctttgccc acgccttcta
    21661 ccggatcttc ggctacgagc tcgccaccga agccatcgag tacaggctaa agcccgccga
    21721 cctcccgatc agccccgaac agagtcatct cagggcctac cggcaagaag atctttcctc
    21781 tttgatggat ctctacgaag cggaggcgaa agggcatacc ctcagcgtac gcaggagcga
    21841 aggacactgg aaaacaagtc tgtccaagaa agacacggac gtcgccgtct atgagcggga
    21901 cagtgacgtg gaaggctaca tcctatacaa gatctcaggg tggcaagacc gcgacccgcg
    21961 gcgcaccctc tcggtagatg agatcgttgc cgcaacaccg cgggcgagag aggcactctt
    22021 gtcctttatg gcaggcctcg atcccctggt cttcggtata aagctataca ccccgcgcgg
    22081 agagccactt cacccctacc tgagaagctc gcacgtagac gcgaagatcg agccagatca
    22141 aatgctgcgc ctcgtcgacg tcgaaggcgc cctcgggtac ctcgagcacg tgccggaggc
    22201 gccgctagta ctcgacatat cagacgacgt cataccggag aactccggtg agtacaccgt
    22261 cggaaacggc aaagttttcc gcggagcgga agcggcggag agcgtctccc tcgacgtacg
    22321 ccagctcgcc cagctctacg ccgggtacct ttcggtacgg agtcttgcca ggcatgggct
    22381 tatcaaggca agccctcccg aagctctcga actcctcgag gcgctctttc cccagggaga
    22441 cccatggctc tatgggccgg atcatttttg attagcatgt catccagtca gcaagaactt
    22501 acgcaaggga gggctgaaat gctgccgacg aaggaggcgc aatgaaaccc acgggtaccg
    22561 taggctacgc tgcgatgttc gagcagttcc atcccacgga tcttctgaat tggagtaaga
    22621 cggctgaaga gtcggggttc ggctccgtga tggcctctga tcacttccac ccgtggaccc
    22681 cggatcaggg acagagcgcc ttcgtatggt cctggatggg cgctttgggc gccagcacga
    22741 acctccgctt cggtacgggc gtgaccccgc ccggcttccg ctaccatccg gccataatcg
    22801 cccagggagc ggcgacgctg gaagcgatgt acccgggccg cttctggctc ggcctcggag
    22861 cgggtgaggc gctaaacgaa cacatcgtcg gcgattactg gcctgaggct ccgacaaggc
    22921 tccgcatcct gatggaggcc atcgaggtga tccgcaggct cttctcgggc aaggtcgtca
    22981 agtacagtgg cgagcacata aaactcgaga gcgccagact ctacacgatg cccgagatac
    23041 cgcctccgat ctacgtggcc acggcaggtc ctttacagag ccgcaggact ggcgagttct
    23101 gcgacggcat catcaccgtc ggcgccccag acgagaagat aaagaacctg atggagaagt
    23161 tcgaggaggg agcgcgccgc gcggacaagg acccctccga gatgccccgc atgatacagc
    23221 tccacgtctc ctgggccgag gaccaggaag aggccgagca gcaggccgtc agagagtggc
    23281 ccaacggcgg gatgcccttt cccaaggcag atataaggaa ccccgaggac ttcgcagcga
    23341 tggcaaagca ggtcggcgtc gagaacttcg agaaccgcgt cctcatgagc gccgacctcg
    23401 acgagcacct cgagcgcgtc cagcactacg tagacctggg cttcgacgag gtatacatcc
    23461 acaacgtcgg gcgcaaccag gagcagttca taagagccta tggcgagcgc gtcatcccca
    23521 agctccgctg gccggagggg taactcccct ggcagggtcg gcgttcctac tgtctctacg
    23581 ggcagttttg atccagccca ggcaggccgt cgatgcttcg agtgttcttg agtgctcggt
    23641 attcggccat cttctcgggg cctttgcgct cggctgaggt caggagattg tcgcgcgggc
    23701 cgacgaggtc catgcgcggg acgccgaacc cgcacgagtc ggcgatcctg cccacgcaca
    23761 cgtcgatgat cgcgcgttcg cccggaaggt cggggaagct ggagcgcagc tcgtcgtatc
    23821 cggcgccacc ggactctagc acctcgcctt gtccgtagag gcgcaggatc ttcggcagtc
    23881 cctcgaaagc gcagaacatc agcgtgatcc tgccgtcagc cctgaggtgg gctatggtct
    23941 cgacgccgct gccggtgagg tccaaccacg ctacccgctt cggcccaagc accctgaacg
    24001 tgtcaagccc ccgcggcgag acgttgacgt gtgcgccacg ggcggcggtg gcgacgaaga
    24061 acatcggctg ctttccgatc cagagcgcca gctcctcgct gatgtcgtcg tagactctgc
    24121 ccatcgtccc gatcctccct gttttgctct tcgtggatct tccactgcta aggattgtac
    24181 gctcggactg tggcagtatg cgctcgctct gtcagtattt gtggcgtttc ctgaaaactc
    24241 ggcccgtgtg tatcgatggt ggctgcagat ggaggggagt aactttggaa tctggatctg
    24301 aaggaactct gaatgatacg atcctgctcg gtgtggaggc gcgttgaagg tcgcggttat
    24361 aggagccggg atcgtcggct cttcggtcgc ctactggctc tccgagggcg gggctgaggt
    24421 tgttatgatc gacggcgcgg aaccgggttc c
//
LOCUS       DASTKM010000194         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1289430, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000194 DASTKM010000000
VERSION     DASTKM010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289430"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..407
                     /locus_tag="VFQ10_08050"
     CDS             123..407
                     /locus_tag="VFQ10_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965332.1"
                     /translation="MSVEERHSREMMQQDLMDVGVWLVEHPEAATKNLEPIRSVKVLE
                     PGVYYNMGTGMVDRIFTPQRVALGHKMFRISRDPASPAEDIRRNALEGKT"
     gene            407..1243
                     /locus_tag="VFQ10_08055"
     CDS             407..1243
                     /locus_tag="VFQ10_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9965333.1"
                     /translation="MATGFLTDTTVCIGCKACEVACKQWNQLPADGYELTGDSYDNTK
                     QLSGTTWRHVAFIENPKKRRLTGDVDLVALAEDVTEPLPGGAELDALGRWAFMSDVCK
                     HCVAAPCQQACPTGAIIRNEFDNVYIQPDICNGCGYCVPACPYGVITQVPHDGRAFKC
                     TLCYDRQRDGLEPACAKACPTNSIMFGEVEELKAIAEERVEELHDKGYSQAYVWGTPE
                     VGGTGGIGGNHAKFILMDEPETYNLPRHPFLPQEKVKGGTIASMVAGAVLGAVVALAF
                     RD"
     gene            1254..2291
                     /gene="nrfD"
                     /locus_tag="VFQ10_08060"
     CDS             1254..2291
                     /gene="nrfD"
                     /locus_tag="VFQ10_08060"
                     /inference="COORDINATES: protein motif:HMM:NF025940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9965334.1"
                     /translation="MSEKTQDGVQQSNGKVETPHHHETDPRREDKNYYGIPPIKHAHW
                     TWQIPVYFWIGGIGAGVHLFSTMAQVLGHRDAALTRTSRYTVLGTMILSPILLIWDLG
                     RPERFYNMLRILKLRSPMSTQSWSVFAFGNLGGLIAARQAAEDGLLGDDNFLVRLVKL
                     IPARALSILAVPVGLNVGSNTGNLISATSVPLWARNWLFMGPTFLASGLSTALSWLSL
                     VLHLTRSGEEKTLRTLHRAEKATIVIEAGLIAASLARMSRWSKPLFSREVAPLFVGGT
                     LIGGIVAPMALLFGKESHSKSMLASVLALAGGLAFRFAIIKGGRISADDPEAYFTFAS
                     GESVPQPEDKV"
     gene            complement(2288..2692)
                     /locus_tag="VFQ10_08065"
     CDS             complement(2288..2692)
                     /locus_tag="VFQ10_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9965335.1"
                     /translation="MKVVRARDMQTGPGPEEWFTGKVWRDAAPAGASPDVAVNRVLFE
                     PGARTHWHTHPEGQILYIITGTCRAGKEGEGPIEVEAGGVVYFAPDEKHWHGATPETY
                     MVHMALNVATTTDGGTDWLEPVTDEEYGSRRP"
     gene            complement(2760..>3032)
                     /locus_tag="VFQ10_08070"
     CDS             complement(2760..>3032)
                     /locus_tag="VFQ10_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9965336.1"
                     /translation="LRNPLGLPERLAMRFGWSKRGRRVGEALSRLAGVQEPNVRWHLV
                     HEAPWFHNHVSSLEIRGQEATLKVEKTMPENNDEPQLYRILEHRLA"
BASE COUNT          559 a          974 c          949 g          550 t
ORIGIN      
        1 actggcgtcc acagggcgcc ctggacccag aagacgccga aagtcctgct ccccgtgata
       61 gaccacgcgg tattcggcgc agcctgggga ttcctctact gggcgctccg caaggcgcgt
      121 gcgtgagcgt cgaggagcgc cacagtaggg agatgatgca gcaggatctg atggacgtcg
      181 gagtctggct agtcgagcat cccgaggcgg ctacaaagaa cctcgaaccc ataaggagtg
      241 tgaaggtact cgagccgggg gtctactaca acatgggcac ggggatggtc gacaggatct
      301 tcacacccca acgtgtggcc ctcgggcaca aaatgttccg tataagcaga gatccggcgt
      361 cgccggcgga ggatatccgg cggaacgcgc tagaagggaa gacctagtgg cgacaggatt
      421 cctgaccgat acaacggtct gtatagggtg caaggcctgc gaggtcgcct gcaagcaatg
      481 gaaccagctc cctgccgacg gctacgagct gacgggcgac tcctacgaca acacaaagca
      541 gctctcgggc actacctggc gccacgtcgc gttcatcgag aacccgaaga agcgtcgtct
      601 gaccggcgat gttgacctcg tggcgctcgc cgaggacgtg acggagccgc tgcccggcgg
      661 agccgagctc gacgccctgg gacgctgggc ttttatgagc gacgtgtgca agcattgtgt
      721 cgcggcgccg tgccagcagg cctgcccgac aggcgccatc atccgcaacg agttcgacaa
      781 cgtctacatc cagcccgaca tctgcaacgg ctgcggctac tgcgtccccg cctgccccta
      841 cggcgtcata acccaggttc cacacgacgg ccgcgctttc aagtgcaccc tctgctacga
      901 ccgccagcgc gacggcctcg aaccggcctg cgccaaagcg tgcccgacca attccatcat
      961 gttcggcgaa gtcgaggaac tgaaggctat cgccgaggag cgggtagaag agctccacga
     1021 caagggctac agccaggcct acgtatgggg tacgcctgag gtgggcggaa caggcggtat
     1081 cgggggcaac cacgccaagt tcatactcat ggacgagccc gagacctaca accttccccg
     1141 tcatcccttc ctgccgcagg agaaggtcaa gggaggaacc atagccagca tggtggcggg
     1201 cgcggtactc ggcgcggtag tggcgctggc gttcagggac tagggggacc aaggtgagcg
     1261 agaagacgca ggacggcgtc cagcagagca acgggaaggt cgagaccccg caccatcacg
     1321 agacggaccc caggcgcgaa gacaaaaact actatggcat cccgcccata aagcacgccc
     1381 actggacgtg gcagatcccg gtctacttct ggatcggcgg gatcggggca ggggtacatt
     1441 tgttctccac gatggcccag gtactcggcc atagagatgc ggcgctcacc cgcacgagcc
     1501 gttacacggt actgggcact atgatcctga gcccgatcct tttgatctgg gatctcgggc
     1561 gtcccgagcg cttctacaac atgcttcgca tcctcaagct ccgctccccg atgtccaccc
     1621 agagctggtc ggtcttcgcc ttcggcaacc ttggcggcct gatcgccgcc cgtcaggccg
     1681 ccgaagacgg cctgctagga gacgacaact tcctggtcag gctcgtaaag ctcatccccg
     1741 cgcgtgctct ctctatcctg gcggtgcctg tcgggctcaa cgtcggctcg aacacgggaa
     1801 acctgatctc cgccacctcc gtaccgttgt gggcgcgtaa ctggctgttc atgggcccca
     1861 cgttcctggc ctcgggactc tccacagccc tatcgtggct ctcgctcgtg ctccacctca
     1921 cgcgctcggg cgaagaaaag actctccgta ccctgcaccg cgccgagaag gccaccatcg
     1981 tcatcgaggc agggctcata gccgcctccc tggcgaggat gagcaggtgg agcaaaccgc
     2041 ttttctccag agaggtagcc ccgctcttcg ttgggggcac cctcatcggc ggcatagtcg
     2101 cgccgatggc cctgctcttc ggcaaggaga gccactccaa aagcatgcta gcgtcggtgc
     2161 tcgcgctcgc cggcggcctg gccttccgct tcgctatcat aaagggaggc aggatcagcg
     2221 ccgacgaccc agaagcctac ttcaccttcg ccagcggcga gagcgtcccg cagccggagg
     2281 acaaggtcta ggggcgtctc gagccatatt cctcgtccgt aacgggttcc agccagtctg
     2341 tgccgccgtc tgtcgtggtg gcgacgttga gggccatgtg gaccatgtac gtttctggtg
     2401 tggcgccgtg ccagtgcttc tcgtccgggg cgaaatagac gacgccaccc gcctcgacct
     2461 cgatgggacc ttcgccttct ttgccagcgc ggcaggtacc cgtgattatg tagaggatct
     2521 gcccctccgg atgggtgtgc cagtgtgtgc gggcaccagg ttcgaagagg actcggttga
     2581 cggccacgtc gggtgaggcg ccggcgggag cagcgtctct ccataccttt ccggtgaacc
     2641 actcctcggg cccaggcccg gtctgcatgt ctctcgctct gactactttc atgtgagcct
     2701 cccttcggtc ggcggcttgg gtctcagcct aatgattttt gctgacagct gactgctttc
     2761 tatgccagac ggtgttcgag gattctgtac agctgcggtt cgtcgttgtt ctccggcatt
     2821 gtcttctcca ccttcagcgt tgcttcctgg cctcggatct cgaggctcga gacgtggttg
     2881 tggaaccagg gcgcttcgtg caccaggtgc cagcggacgt tcggctcctg gacgccggcc
     2941 agtcgggaga gcgcctcgcc gacgcgcctg ccgcgcttcg accagccgaa acgcatggcg
     3001 aggcgttcgg ggaggcctag cgggtttcgc ag
//
LOCUS       DASTKM010000195         3920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1112774, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000195 DASTKM010000000
VERSION     DASTKM010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112774"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /gene="dnaK"
                     /locus_tag="VFQ10_08075"
     CDS             <1..1544
                     /gene="dnaK"
                     /locus_tag="VFQ10_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET9965337.1"
                     /translation="KRDAEEYLGEEVSEAVITVPAYFEDAQRQATKDAGRIAGLNVKR
                     IINEPTAASLAYGMDKVEDQTILVFDLGGGTFDVSILELGEGVFEVKATSGNNHLGGD
                     DFDAKVVEWMVSEFKKVEGIDLSKDKMAAQRLVEAAEKAKRELSSTTSTSINLPFITA
                     DQNGPKHMDLTLTRAQFNNLTADLVEATAGPVRQAMEDASLQPGQVDQVILVGGSTRI
                     PAVQEQVKEITGKEPHKGINPDEVVAVGAAIQAGVLAGEVKDVLLLDVTPLSLGIETK
                     GGVFTKLIERNTTIPTRKSEVFTTADDNQGSVEIKVYQGEREIAAYNKIIGNFQLVGI
                     PPAPRGVPQIEVAFDIDANGIVNVGAKDLGTGKEQRITITASSGLSDAEIQQMMQDAE
                     SHAEEDRQRREEADVRNNADNLVYSIERSLKDVDGKVDPNTKLEIEQAIKEAKEALAG
                     GDVEEIKRKQENLMTASHKLSEVLYSQAAQQQEASSAGATDTDDDLVEDAEVVDEDED
                     SEERK"
     gene            1612..2253
                     /locus_tag="VFQ10_08080"
     CDS             1612..2253
                     /locus_tag="VFQ10_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET9965338.1"
                     /translation="MSSEENKRDPELGDDAWQEDGAVEEAEDSPVDPASLENGESPGA
                     PEDAEVEPATDEEPDPEWDEAAVLSVELEATRRDRDQYLDALQRLKAEFENSRKRQER
                     ERIRILSMASERLVQELLPVLDNLDRALEAGGDIREGVQATRDQLADVLGEEGLLPVA
                     SDGQPFDPNVHEAVMGQVSEEHEEGTIIQTFQRGYLLNGKPIRTAKVVVAKQV"
     gene            2257..3366
                     /gene="dnaJ"
                     /locus_tag="VFQ10_08085"
     CDS             2257..3366
                     /gene="dnaJ"
                     /locus_tag="VFQ10_08085"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET9965339.1"
                     /translation="MEKLDLYKVLEVSKDAPQDEIRRSYRRLARKYHPDANPGDKRAE
                     ERFKEVQHAYEVLSTPEKRREYDEGPRTFFGSQGAGQPSGANFEDFSDLLGGFGNLGD
                     IFGRSASAAQRTRAKGESITVNVRLSFEDALRGVTTRVGVPVEEACGECRGTGAAPGT
                     APLTCPECGGRGVQSRDQGFFALSTPCSRCGGEGRIIEKPCTVCGGNGRVRRNRQVKV
                     RIPAGAKDGMKVRIPGRGSAGRRGGPAGDLYVVTRVEEHPVFRRRGDDFVVEVPVSFV
                     EAVLGAQIEVPRPGGGTVRVKLPAGTQDGKQFKVRGAGAPRTKKNGAENGNLIVRVGL
                     VVPKKLKRREREILEALAEERDEDVRQDLFKKVRS"
     gene            3363..3764
                     /locus_tag="VFQ10_08090"
     CDS             3363..3764
                     /locus_tag="VFQ10_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9965340.1"
                     /translation="MSLRKRPVYMIGIASELIGVHPQTLRMYEQKGLLRPRKSIKNTR
                     LYSQEDVDLGRYIQRLTQEMGMNLAGVRKVIDLERHVARLEAETRALREELDRKDAEH
                     SRLIAEVHQSYKRELVLMPRGELARSRRNDR"
BASE COUNT          878 a         1102 c         1362 g          578 t
ORIGIN      
        1 tgaagaggga tgccgaggag taccttggcg aggaggtcag cgaggcggtc atcacggtgc
       61 ccgcgtactt cgaggatgcc cagcgccagg cgaccaagga cgctggcagg atcgccgggt
      121 tgaacgtcaa acggattatc aatgagccga ccgcggcatc gctggcctac ggcatggaca
      181 aggtggagga ccagaccata ctcgtcttcg acctgggtgg cggtactttc gacgtctcca
      241 tcctggagct cggcgagggg gtcttcgagg tcaaggccac gagcggcaac aaccacctgg
      301 gcggcgacga cttcgacgcc aaggtggtcg agtggatggt ctcagagttc aagaaggtag
      361 aaggtataga cctctccaag gacaagatgg ccgcccagcg cttggtcgag gccgccgaga
      421 aggccaagag ggagctctcg tcgaccacca gcacctcgat aaacctgcct ttcatcacgg
      481 cggatcagaa cggtccgaag catatggacc tgaccctcac aagggcgcag ttcaacaacc
      541 tgaccgctga cctcgtcgag gctacagccg gccccgttcg ccaggcgatg gaggacgcta
      601 gtctgcagcc tggacaggtc gaccaggtga tactcgtcgg cggctcgacc cgtatccccg
      661 cggttcagga gcaggtcaag gagatcacgg gcaaagaacc ccacaagggc atcaaccccg
      721 acgaggtcgt cgcggtcggg gccgccatcc aggccggagt gctcgcagga gaggtcaagg
      781 acgttctgct cctcgatgtc acgccactct ccctcggtat cgagaccaag ggcggggtgt
      841 tcaccaagct catcgagcgg aataccacta tacccacacg gaagagtgag gtattcacca
      901 ccgctgacga caaccaggga tccgtcgaga tcaaggtcta ccagggcgag cgggagatag
      961 cagcgtacaa caagatcatc gggaacttcc agctcgtcgg catcccgccc gcgcccaggg
     1021 gcgtacccca gatcgaggtc gctttcgaca tcgacgctaa cggcatagtc aacgtcgggg
     1081 ccaaggacct ggggactggc aaggagcaga ggatcacgat cacggcctcg agcggcctct
     1141 cggacgcgga gatccagcag atgatgcagg acgcggaatc gcacgccgaa gaggacaggc
     1201 agcgtcgcga ggaggctgac gtccgcaaca acgcggacaa cctggtgtac tccatcgagc
     1261 gttcgctgaa ggacgtggac ggaaaggtgg acccgaacac caagctggag atagagcagg
     1321 ccataaagga ggccaaggag gcgctcgccg gcggcgacgt cgaggagatc aagcgcaagc
     1381 aagagaatct gatgaccgcc tcccacaagc tctcggaggt tctctacagc caggcggccc
     1441 agcagcaaga agccagctcc gccggggcca cggatacgga tgatgacctg gtggaggacg
     1501 ccgaggtcgt cgacgaggac gaggacagcg aggagcgcaa gtaaataggg ccggccgggg
     1561 tgagatcacc ccggccgcct gcgcggcatg gtacaggagg actaggcagc tttgagcagt
     1621 gaggagaaca agcgagatcc cgaactcggg gatgacgcct ggcaggaaga cggagcggta
     1681 gaggaagccg aggactcccc ggtcgaccct gcgtctctcg aaaacgggga gagccctggc
     1741 gcgccagaag acgccgaggt cgaaccggcc accgatgagg agccagaccc tgaatgggat
     1801 gaggcggcgg tgctttcggt agagttggag gccacgcgcc gggatcggga ccagtacctc
     1861 gacgccctgc agcgcctgaa ggccgagttc gagaactcgc gcaagcgcca ggagcgggaa
     1921 cgcatccgca tactatcgat ggcatcagag aggctggtgc aggagcttct ccccgtcctc
     1981 gataacctgg accgcgcact cgaggccggg ggagacatcc gcgagggtgt tcaggctacc
     2041 agggaccaac tcgccgacgt actcggcgag gaaggtcttc tgcccgtcgc ctcggacggc
     2101 caaccgttcg acccgaacgt ccacgaggcc gttatgggcc aggtctccga ggagcacgag
     2161 gaaggcacca tcatccagac cttccagagg ggctatctac tcaacgggaa gccgattcgg
     2221 acggcgaaag ttgtcgttgc gaagcaggtt tagggtatgg agaagttgga tctctacaag
     2281 gttctggagg tatccaagga cgcccctcag gatgagatcc gacgctccta ccgcaggcta
     2341 gcacgtaagt atcatcccga cgccaaccct ggcgacaaac gggccgaaga gcgcttcaag
     2401 gaggtacagc acgcctacga ggtcctttcg acacccgaga agagaaggga atacgatgaa
     2461 ggtccccgca ccttcttcgg ctcccaaggc gccggacagc caagtggcgc gaacttcgag
     2521 gacttctcgg atctactcgg cggcttcgga aacctcgggg atatcttcgg acgtagcgcc
     2581 agtgcagccc agaggaccag ggcaaaaggc gagagtataa ccgtaaacgt caggctgagt
     2641 ttcgaagatg ctctgcgcgg tgtaacgacg cgggtcgggg taccggtcga ggaggcatgc
     2701 ggtgagtgca ggggcacggg tgctgctcca gggacggcgc cgctgacgtg tccggagtgt
     2761 ggtggcaggg gtgtgcagag ccgggaccag ggttttttcg cgctctcgac gccgtgctcc
     2821 aggtgtggtg gggagggcag gatcatagag aagccgtgca cggtgtgcgg cggcaacggc
     2881 agggtacgca ggaacaggca ggtcaaggtc aggattccgg ctggggcgaa ggatggcatg
     2941 aaggtcagga tccctggcag gggaagcgct ggcaggagag gtggtcctgc gggagacctc
     3001 tacgtcgtga cgcgggtcga ggagcatccc gtcttcaggc ggcgtggcga cgactttgtg
     3061 gtcgaggttc ccgtcagctt cgtcgaggct gtgctcgggg cgcagatcga ggtgcccagg
     3121 cctggcggcg ggacggtgag ggtcaagttg ccggccggta cgcaggacgg caagcagttc
     3181 aaggtgaggg gcgcgggagc gcccaggacc aagaagaatg gtgcagagaa cggcaacctc
     3241 atcgtgcggg tgggcctggt cgtgccgaag aagctcaagc ggcgggagcg tgagatcctg
     3301 gaggccctcg ccgaagagcg ggatgaggat gtcaggcagg atctcttcaa gaaggtgaga
     3361 tcgtgagcct cagaaagcgt cctgtgtaca tgatcgggat agcctccgag cttatcgggg
     3421 tgcatccgca gaccctaagg atgtacgagc agaagggtct tctccgtccg cgcaagagca
     3481 taaagaacac caggctctat tcgcaggagg acgttgacct cggacgctac atacagaggc
     3541 tgacccagga gatgggaatg aacctcgccg gcgtgaggaa ggtaatcgac ctcgagcgcc
     3601 acgtagcgag gctggaggcc gagacccgtg ctctgcgcga agaactggac aggaaggacg
     3661 cggagcatag ccggctgatc gctgaagtcc accagagcta caagagggag ctggtcctga
     3721 tgccccgtgg cgagcttgcg cggagcagaa ggaatgatcg ctgatggccg agcccatgta
     3781 ctgctaccgg catccgaaaa gggagactcg cgtctcatgc gccacgtgcg ggcggcccat
     3841 ttgcaccgag tgcatggtgg ccaccgacgt cgggataaaa tgccccgacg atgccaggct
     3901 accaagagga gcgcgggccg
//
LOCUS       DASTKM010000196         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_936831, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000196 DASTKM010000000
VERSION     DASTKM010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936831"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFQ10_08095"
     CDS             complement(<1..722)
                     /locus_tag="VFQ10_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET9965341.1"
                     /translation="MAHGEGGRTIVVSNRGPVTFTRGESDEREHSRGAGGLVTALNAV
                     LRRTENAVWIASAQSEEDIAVSKEPAPYEIEDLRVRFVEHDPDAYDLMYNELANPLLW
                     FVQHGLYDLPYSPALGDETRRAWEEGYVPVNRNFADAVVETAAGQDSPTVLLHDYQLY
                     MTPLFVRERLGAGAFLSLFVHIPWPEPDLWRVLPHYVREGVLESLLSADVVAFHTHRY
                     AHAFAETAADVLGAKAEGGVIR"
     gene            complement(746..1996)
                     /locus_tag="VFQ10_08100"
     CDS             complement(746..1996)
                     /locus_tag="VFQ10_08100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9965342.1"
                     /translation="MQQRVNPGNKSLADYRSIVRRELYDEIVALGERLRGKRVLHVSA
                     TSFGGGVAEILYTLVPLMRDAGLETEWDIMFGSEPFFNVTKSFHNALQGAEYELTVED
                     RAIYEEYNRISAEALQKSGEDWDIMFVHDPQPALLKHFSGGLSPQTKWIWRCHPDLST
                     PNRHVLDYLLPHIADYDAQIYTMEEYTPPDVHLPGLTLIPPAIDPLSPKNMALSTDDA
                     RYIVSQFGVDVEKPFMVQVSRFDPWKDPLGVIDAYRLVKEEVPEVQLVLIGSMAHDDP
                     EGWDYWYKTVNYADGDRDIFLFSNLTNVGAIEVNAFQSIADVVIQKSIREGFGLVVTE
                     ALWKARPLVAARVGGIPMQVDGGGGILIDTIPEVASACTKLLKDRDFAREMGRHGKEH
                     VRTHYLTPRLLRDDLLLFAKLLGV"
     gene            complement(2127..2558)
                     /locus_tag="VFQ10_08105"
     CDS             complement(2127..2558)
                     /locus_tag="VFQ10_08105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9965343.1"
                     /translation="MERETARIVEEGSGLSDPISLTLPSSPEYVLLARLVMGQAGRLA
                     GLEPEEVYDLKLAVTEAVTNVIRHASVDSYEIEYRILPLGVEISVTDAGGGFSVADLT
                     GESDEHGGFGLTVIRNLVDELVLDSTKGGGTRLKMIRRVHG"
     gene            complement(2816..>3028)
                     /locus_tag="VFQ10_08110"
     CDS             complement(2816..>3028)
                     /locus_tag="VFQ10_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965344.1"
                     /translation="NSTIILPVPIDLIRPFMESQNGASSSYGQEVRAARREEEKEAER
                     LYEEAVGEVSPQASPDEVTSDEVRPE"
BASE COUNT          539 a          944 c          941 g          604 t
ORIGIN      
        1 taccggatca caccgccctc ggccttggcg ccgagtacat cggcggcggt ctcggcgaag
       61 gcgtgggcgt agcggtgggt gtggaaggct acgacgtcgg ccgagaggag gctctcgagc
      121 acaccctccc ttacgtagtg cggcaggacg cgccagaggt caggctcggg ccaggggatg
      181 tggacgaaca gggagagaaa ggcgccagct ccgagacgct cacggacgaa gagcggggtc
      241 atgtagagct ggtagtcgtg gaggaggacg gtgggcgaat cctggccggc tgcggtctcg
      301 accacggcgt cggcgaagtt gcggttgacg gggacgtagc cctcctccca ggcccgcctc
      361 gtttcgtccc cgagcgcggg cgagtaaggg agatcgtaga gcccgtgctg gacgaaccac
      421 aggagcgggt tggcgagctc gttatacatc aggtcgtagg cgtcagggtc gtgctctacg
      481 aacctgacac gcaagtcctc gatctcgtat ggcgccggtt ccttcgagac tgcgatgtcc
      541 tcctcgctct gggccgaggc gatccagacc gcgttctcgg tgcgtctgag gaccgcgttc
      601 agggcggtga cgaggccccc cgctcccctg gagtgctcgc gctcgtccga ctcgccgcgg
      661 gtgaacgtta caggccccct gttcgagacc acgatggtcc tcccgccttc gccgtgcgcc
      721 atggtccccc tctcgttcgc ggctgctata ccccgagcag tttagcgaaa agaagaaggt
      781 cgtcgcggag gagacgcggc gtgaggtagt gcgtcctcac gtgttccttc ccgtgacggc
      841 ccatctcgcg agcgaagtcg cggtccttga ggagcttggt gcacgcggag gccacctcgg
      901 ggatggtgtc gatcaggata ccgccccccc catctacctg cattgggatg ccgccgactc
      961 tggccgcgac cagcggcctc gccttccaga gcgcctctgt cacgacgagc ccgaagccct
     1021 cgcggatgga cttctgaatc accacgtctg ctatggactg gaaggcgttg acctcgatgg
     1081 ctccaacgtt tgtgaggttg gagaacagga agatgtcgcg gtccccgtca gcgtagttga
     1141 ccgtcttgta ccagtagtcc caaccctcgg gatcgtcgtg ggccatcgat ccaatcaaga
     1201 cgagctggac ctcggggacc tcctctttta cgagcctgta ggcgtctatg acgccaagag
     1261 ggtccttcca tgggtcgaag cgcgagacct gcaccataaa aggcttctcc acgtccacgc
     1321 cgaactggct gacaatgtag cgggcgtcgt cagtgctcag ggccatgttc ttgggtgaga
     1381 gtgggtctat cgccgggggg ataagggtga gcccgggcag gtgcacgtca ggaggggtgt
     1441 actcctccat ggtgtaaatc tgcgcgtcgt agtccgctat gtgcggcagt aggtagtcga
     1501 gcacgtgccg gttgggggtc gagaggtccg ggtggcagcg ccagatccac ttcgtctgcg
     1561 gcgaaagccc ccccgagaag tgcttgagca gggccggctg cggatcgtgg acgaacatga
     1621 tgtcccagtc ctcgccggac ttctggagtg cctcggcaga gatgcggttg tactcttcgt
     1681 agatcgcccg gtcctcgacg gtaagctcgt actcggcgcc ctgcagggcg ttgtggaagc
     1741 tcttggtgac gttgaagaaa ggctccgacc cgaacataat gtcccattcg gtttcgaggc
     1801 cggcatcccg catcagcggt acgagcgtgt acaggatctc agcgacccca ccaccgaagc
     1861 tcgtcgcact gacgtgcaga acccgcttcc ccctcagacg ctccccaagg gccactatct
     1921 catcgtagag ctcccgacgg actatgctgc gataatcggc gagcgatttg ttgccgggat
     1981 ttacacgctg ctgcactgtc taccttctag cccctctcgg cgttctggca cgctaagtac
     2041 acggtgaact gtatattaac gcgcaccgaa gattcaactg gatagatcat gaaacgccaa
     2101 ggagtgcctt tccctggatt tccaccctat ccgtggacgc gccggatcat cttcagccgc
     2161 gtccctccac ccttggtcga gtcgagcacc aactcgtcca ccaggttgcg gatcacggtg
     2221 aggccgaacc caccatgctc gtccgactcg cccgtgagat ccgcaacgct gaaaccgccg
     2281 ccggcatccg tgacgcttat ctcgacaccc aacggcaaga tcctgtactc gatctcgtag
     2341 ctatccacag aggcgtggcg gatcacgttg gtcaccgcct cggtaacggc gagcttgagg
     2401 tcgtagactt cttccggttc gagtcctgcc agacgtccgg cctggcccat caccaaacgg
     2461 gcgagcagca cgtactccgg gctgctcgga agggtcagtg agatcggatc cgatagtccg
     2521 ctcccctctt ccactatcct ggctgtttca cgctccatcg gacctctcgg tcatccggct
     2581 cccctcacgc aaactgacga aatagcttgc cggaagcgta actttgcaac ttcgggaatc
     2641 ttcttaccca gatgtatacg acgctaaccc gccgcgtgga ccggcgggct gcagggggtt
     2701 ggagtcatgg gcggcgccag attggctcgt tgttttggtc cttagcggct cactaacagc
     2761 cgaatccctg attcggagca gctcaatccc gctgcaaatc gctctaggta cgttctcact
     2821 ctggtcgcac ctcatcggaa gtcacctcat ccggcgacgc ctggggtgag acttctccta
     2881 cggcctcctc gtagaggcgc tcggcctcct tctcctcctc gcgccgtgcc gcccttactt
     2941 cctggccgta ggaggaggac gctccgttct gggactccat aaagggtcgg atgaggtcta
     3001 tgggaacggg caggatgatg gttgagtt
//
LOCUS       DASTKM010000197         5297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1289884, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000197 DASTKM010000000
VERSION     DASTKM010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289884"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFQ10_08115"
     CDS             <1..317
                     /locus_tag="VFQ10_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965345.1"
                     /translation="LLQRLFVALTGQQSTLAVVASTLLIAALFNPLRRSIQSFIDRRF
                     YRRKYDARKALEAYSLKLRNETDIEALNDDLVGVVRETMQPAHVSLWLHPPTLPKSKE
                     SD"
     gene            430..786
                     /locus_tag="VFQ10_08120"
     CDS             430..786
                     /locus_tag="VFQ10_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965346.1"
                     /translation="MLALFVLPLILAFAPAAVWAFRAGGVRRLWLLGAFALLALILTA
                     LVLSAAYSVPSVWRVVLYFLVFVGPSILFTNAALTVAGAFERALWTQLITAFVGSLVG
                     LAVGFVAAVYGLGVWS"
     gene            927..1601
                     /locus_tag="VFQ10_08125"
     CDS             927..1601
                     /locus_tag="VFQ10_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HET9965347.1"
                     /translation="MSAEREIPLALMVQTPDEPGVLHALTKVFLDHEANITHVDIAER
                     REGLSTVYFELEDVAGEAAEVLIADLKELPVVTAAERAPSFQKVYGKRIIVVGGGAQV
                     GQVVVGAVAEADRHNIRGEKISVDTIPLVGEEELAAATRAVARLPRAVALVLAGALMG
                     GEVTRAVHEIREKGIIVLSLNMPGSVPDAADLVVSDPVQCGVMAVMAVSDSALFDITR
                     QRGQRY"
     gene            1716..2381
                     /locus_tag="VFQ10_08130"
     CDS             1716..2381
                     /locus_tag="VFQ10_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9965348.1"
                     /translation="MRLVIFGPTGGTGRRLVERAIAEGHEVTAFVRNPSRMSARHRRL
                     EVVVGDVSDAGKVREAVDGNEAVISVLGSRQPSNPLHPHRPGNPHGPVSAGSANIIAA
                     MKEHGLRRFVCQTAWGAGESKQDPGFAGAFFMNVLVPPLLRDEYAEKEAQEKLVRESD
                     LEWIIVRPMILTNGPWTNDYRVGLDLKPGRRPYISRADVADFLMKQLTDDTFVHRTPA
                     IGY"
     gene            2486..4237
                     /locus_tag="VFQ10_08135"
     CDS             2486..4237
                     /locus_tag="VFQ10_08135"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9965349.1"
                     /translation="MTPLDTLSDNFVRAAWLDDVRAAGRTSVRVAGHSLALFFHDGRV
                     HALDNRCPHMGFPLHRGTMQDGILTCHWHHARFDLESGGTFDQFADEARVFPVEVRDN
                     EVWVDLSPREDRPAYLRQRLRDGLERNISLVVSKSVIGLLGSGEDPAEPFRAGLEFGS
                     RYRRAGWGQGLTMHACMMNLLPHLTPQDRPRALYHGLSAVSRDSAGEPPRFSLRPLPT
                     ETVDLATLKIWFRRFVEVRDSEGAERCIVSAVRTGADPAQMADLLFSAATDHRYLDSG
                     HVLDFTNKALEALDIAGWSHAEVVLSSLAEGYVGARRMEESNSWRNPVDLVEILQAAF
                     ERLPNTLQSGRDRRGTWEGREELATELLGEHGGAIAEALLEALREGATEAELAGTVTY
                     AAALRIARFPTTNEFGDWDTSLHTFTFSNAVHQGLRRVPSPELLRGVFDAAMSVHLDR
                     FLNVPPSRVPEPNGSEAAPDDLLAELSTLLDERQKVDASGALVANYLYSGGDADRLLA
                     VMGGLLLREDRNFHTIQAIEAAFRQYALLRDTTVGAHVLVAAARYLAAHSPTMRSQGQ
                     TYDIARRLSRGEILYEE"
     gene            complement(4547..4816)
                     /locus_tag="VFQ10_08140"
     CDS             complement(4547..4816)
                     /locus_tag="VFQ10_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965350.1"
                     /translation="MPTRDFYTLPEAAQILEAPQRSLLKSLETGEIEGERDTQSGRWK
                     IPKQAVDERVSADSSPEDTAEESQGPSAEMIRELVGEFGDLHRHV"
     gene            complement(4892..>5297)
                     /locus_tag="VFQ10_08145"
     CDS             complement(4892..>5297)
                     /locus_tag="VFQ10_08145"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HET9965351.1"
                     /translation="GRVPGEAPEDSPARLPDLALKGIEEFNRGEFYECHEYLEEAWMQ
                     EPGRVRFLYQGILQVGVGFYHLQNGNWRGATGLLRNGAVRLKEFEPVTLGVDVAKLVR
                     ESERCLRELESLGRERIDEFDRSGVPKVEWTE"
BASE COUNT          943 a         1655 c         1636 g         1063 t
ORIGIN      
        1 ttctgctgca gcggctcttc gtcgctctca ccggccagca gtctaccctc gcagtcgtgg
       61 cctctaccct cttgatcgcc gcactattca accccctaag aaggagcatc cagtccttca
      121 tagatcgccg cttctaccgt aggaaatacg acgccaggaa ggcgttagaa gcctactccc
      181 tcaaactgag aaatgagacg gatatcgagg cactgaacga cgacctggta ggcgtggtga
      241 gggagaccat gcagcctgcg cacgtctctc tgtggctaca cccgcctact ctcccgaaga
      301 gtaaggaatc agattagcag ccccttttga cctgctgagg acctacaagg aggagtgtgc
      361 actgcgagtg attcttttct gcgagacgcc aagcgcgtct cccggagcct ggattttggg
      421 gtggaagcga tgcttgcact gttcgttctc ccgctgatcc tggccttcgc cccggctgca
      481 gtgtgggcgt tcagggcggg cggcgtgcgg cggctatggt tgcttggcgc attcgcactg
      541 ctggcgctca tcctcacggc gctcgtgctc tccgccgcat actccgtccc tagcgtgtgg
      601 cgtgtagtgc tctattttct ggtgttcgtg gggccgtcga tcctgttcac caatgccgcc
      661 ctcacggtgg cgggcgcctt cgagagggcg ctttggaccc agctgattac agcgtttgtt
      721 gggagcttgg tcgggcttgc ggtgggcttt gttgcggccg tctatgggtt gggagtgtgg
      781 tcttaggtgc gtcttcgcca cgctagtcgc actctccttc gccgtcatcg tcgtcgtgtt
      841 gcagcggctc ttcgtcgtct tgatcggcta acaatccacc ctcacgagcg aagaagataa
      901 gccggagagg ctatacactc cggtctatga gcgccgagcg tgaaatcccc ctggccctga
      961 tggtccaaac gcccgacgag cccggtgtcc tgcacgccct gacaaaggtg ttcctcgacc
     1021 atgaggcgaa cataacccac gtcgacatcg ccgagcggcg cgaagggctc tccacagtct
     1081 atttcgagct cgaggacgtc gccggcgaag ccgcggaggt gctcatagct gacctaaagg
     1141 agctgcccgt cgtgacggcg gcagaacggg cgccttcgtt ccagaaggtg tacggcaagc
     1201 ggatcatcgt ggtcggcgga ggggcgcagg tgggccaggt ggtggtcggc gcggtcgccg
     1261 aggccgatag gcacaacatt cgcggtgaga agatctccgt cgacaccatc cccctggtcg
     1321 gcgaggaaga gctcgccgct gcgacgcgcg ctgtagcccg cctgccgcgg gcagtggcgc
     1381 tcgtcctggc cggtgcgttg atgggtgggg aggtcacccg agcggtacac gagatccgtg
     1441 agaaaggtat catcgtgctc tcgctcaaca tgccaggcag cgttcctgac gccgctgacc
     1501 tggtggtgag cgacccggtc cagtgcggcg tgatggccgt tatggcagta tccgactcgg
     1561 ctctcttcga catcactcgc caacggggtc aaagatatta gcccgctcgg gaggtacgtc
     1621 gaagtctgcc cagcgactag taggtcgggt acaggggggg attgggtaca cttacgggcg
     1681 gcagcctgcg aaggcggact acagggagtg cgaccatgag gttggtgatc ttcggaccta
     1741 cgggcggcac cgggcggcgt ctcgtggaga gggcgatcgc agaagggcac gaagttaccg
     1801 ctttcgtcag aaatccctcc aggatgagcg cgcgccacag gcggctagaa gtcgtcgtgg
     1861 gggacgtctc cgacgcaggg aaggtgcgcg aggcggtgga cggcaacgag gcggtgatca
     1921 gcgtcctcgg ctcgcggcaa cctagcaacc cgctccaccc tcacagaccg gggaacccgc
     1981 atggaccggt ttcggccggc tccgccaaca tcatcgcggc gatgaaggag catggtttgc
     2041 ggcgcttcgt gtgccagact gcgtggggag caggggagag caagcaggat ccagggtttg
     2101 cgggcgcgtt cttcatgaac gtgctcgtgc cgccgctgct cagggatgag tacgcagaga
     2161 aggaggcaca ggaaaaactc gttcgggaga gcgatctgga atggatcatc gtacgcccca
     2221 tgatactgac caacgggccg tggacgaacg actaccgcgt ggggctggat ctgaaaccgg
     2281 gccggcgccc ttacatctcg cgagctgacg tcgccgattt cctgatgaag cagctcaccg
     2341 atgacacgtt cgtacacagg acacctgcta tcggctattg acgcagggcc attcgaacga
     2401 tcccgttgca gggacatcca aggcagagga aggagctgga ctcctcaccg ttcgtgtcat
     2461 acagtaatgt ccagacggga aagggatgac gccgctggat acactctcag ataacttcgt
     2521 gcgggccgcc tggttggacg atgtgcgtgc cgcaggtcgc acctctgtcc gtgtcgccgg
     2581 ccactcgctc gcgctcttct tccatgacgg tagggtgcac gctctggata atcgctgtcc
     2641 ccacatggga ttccccttgc accggggtac catgcaagac ggcatcctca cctgccactg
     2701 gcatcacgcc cgcttcgacc tcgagagcgg tggaaccttc gatcagtttg ctgacgaggc
     2761 acgcgtgttc cccgttgagg ttagagacaa cgaagtatgg gtggatctct cgccgcggga
     2821 ggaccgtcct gcttacctgc gccaacgcct gagagatggg ctggagcgca acatttcgtt
     2881 ggtcgtgtcc aagtcggtca tcgggttgct gggcagcggg gaagatcccg ccgaaccttt
     2941 tcgcgcgggg ctggagtttg gcagcagata ccggcgagcg ggatgggggc aaggactgac
     3001 catgcacgcc tgcatgatga accttttgcc tcacctgact ccccaggacc ggccacgggc
     3061 tctctaccat gggctctccg ccgtctcacg tgactccgcc ggggaaccgc cacgtttctc
     3121 cctgcgtccg ctaccgaccg agacggtgga ccttgccacg ctcaagatct ggttccgacg
     3181 gttcgtagag gtgcgcgact cagaaggggc cgagcgctgc atcgtctctg cggtgcgcac
     3241 aggggcggat cccgcccaga tggcagatct gctcttctct gccgccactg accatcgcta
     3301 cctggatagc gggcacgtcc tggacttcac caacaaggca ctagaagcac tcgatatcgc
     3361 cggatggtcg catgctgagg tcgtcctctc cagcctggcc gaaggatatg tgggcgcgag
     3421 gcgtatggag gagtcgaact cctggcgcaa ccccgtggat ctggtagaaa tactccaggc
     3481 cgccttcgaa aggttgccta acactctaca gagcggccgg gacaggcgcg gaacgtggga
     3541 gggccgcgag gaactcgcca ccgagctgtt gggagagcat gggggagcca tcgccgaggc
     3601 gctactagaa gcactgcggg agggcgccac cgaagcggaa ctggccggca cggtgactta
     3661 cgccgctgct ctgcgcatag cgcgcttccc aaccacgaac gagttcggcg actgggacac
     3721 ctccctgcac accttcacct tctccaacgc cgtccaccag gggctgaggc gagtcccctc
     3781 tccggaattg ctaagagggg tcttcgacgc cgcgatgagt gtccacctcg accgcttcct
     3841 caacgtcccg ccctcacgtg tgccagagcc caacggttcg gaggcagcgc cagatgactt
     3901 gctggcagaa ctttcaacgc tgcttgatga aaggcagaag gtagatgcat cgggggcact
     3961 ggtcgccaac tacctctaca gtgggggaga cgccgaccgg ctcctggccg tgatgggagg
     4021 cctgctcctc agggaggacc gtaacttcca tacgatacag gctatcgaag ctgccttcag
     4081 gcagtatgcc cttttgcgtg acaccacggt aggcgcacat gtgctggttg ccgccgcgcg
     4141 ttacctggca gcccactctc ccacgatgcg ctctcaggga cagacctacg atatcgcccg
     4201 tcgcctctct cggggtgaga tcctctacga agaatgagcg acaccgtttc ccgaaacctc
     4261 gatacatagc atgctatagc tagcaggggg tctttgacac cttttttcct ccgctccgcc
     4321 acagcaaggc cgaaagggat cctcaatctt gcccaccaaa gagtcttcgc caggacccct
     4381 tggctctctc cagttcctct cgcaacctac tcgcttcttc gtgcagccta tcggcgcgtt
     4441 cgcgttctcg acggtgtccc tgccgctcct gctctagctc ggccaggagc tgctccctct
     4501 cttctgtgtt ggttcgccga acgcggtcca ggcggctcct caggtgtcat acatgcctgt
     4561 gaaggtcgcc gaattcgcct acgagttccc ggatcatctc ggctgatggt ccttgggact
     4621 cttctgcagt gtcctcgggt gacgagtcgg cggatacccg ttcgtctacg gcttgcttcg
     4681 ggatcttcca ccgaccgctt tgcgtgtccc gttcgccttc tatctctccg gtctcgagac
     4741 tcttcaggag actacgctgg ggagcctcga ggatctgcgc cgcctctggg agggtgtaaa
     4801 agtcccttgt tggcatcgtt cgtgctctat tcccgagtct atcgtgcaac cggccctatc
     4861 ccgcgagcgt gcatactaac agatctgctc tctactcggt ccactctacc ttcggcaccc
     4921 ccgaccggtc gaactcgtct attcgctcgc gcccgagcga ctccaactct cgcaggcatc
     4981 gctcgctttc acggacgagc ttcgccacgt cgacgccgag ggtcaccggt tcgaactcct
     5041 tgagacgtac ggcgccgttc cgcaagagtc ccgtagcccc gcgccagttg ccgttctgga
     5101 ggtgatagaa acccacgccg acctggagga tgccctggta gaggaaacgg acgcgtcccg
     5161 gctcctgcat ccaggcctct tcgagatact cgtggcactc gtagaactcg cccctgttga
     5221 actcctctat gcctttgagt gcgaggtcgg gcagccttgc gggagaatcc tccggcgcct
     5281 ctccgggcac ccttccg
//
LOCUS       DASTKM010000198         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_407164, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000198 DASTKM010000000
VERSION     DASTKM010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407164"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..897
                     /locus_tag="VFQ10_08150"
     CDS             124..897
                     /locus_tag="VFQ10_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET9965352.1"
                     /translation="MCAVVRRSPSVQSVDRAVSVMEFLSRRGWSGVTEVARELDIHKS
                     TAYRLLTTLRERGLVEQDAATEKYRLGFGLVLLARAVRADLDILGCARPVCERLSERT
                     RETVTIAVLEDDDAVVIHQSISRASALGVDWTGHHTPLHATAAGKIFLAYMPEDQLLR
                     ILEGPLERFTENTIADPASLKDHTSKIRDEGYGYTVEELEPGLNAVGAPIRRADGAVD
                     GAVSVSGPAFRLPPDALPEIGELSKRAATEISQCLGFQG"
     gene            939..2015
                     /locus_tag="VFQ10_08155"
     CDS             939..2015
                     /locus_tag="VFQ10_08155"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HET9965353.1"
                     /translation="MSGYEDFGSSLRDLPRLRSSRRRRASSWDRSGGNDDRIHVEPGA
                     TKTLAEMGGAGSINHVWITVANEHMDRTPNAIEPDFLRRLLLRMYWDGEEEPSVLVPL
                     GDFFGVGHARTTNFHSAPLQMSPGNGKSFNCFFHMPFAEGARIDLVSELEHENVLFYY
                     YVDYEELDTLEQGLGRFHAQWRRENPTDGVDEDGQSNEEFLFGGENVTGEGNYTIMEA
                     EGRGQYVGCVLNIHNLRDTNHWNWYGEGDDMIFIDGDEWPPSLHGTGTEDYFNTAWCP
                     QEKYSAPYHGITLGGGDNWGGHVSLYRFHVEDPVTFERSIRVTIEHGHANKRSDDYSS
                     VAYWYQAEPHRPFSILPVEQRVPR"
     gene            complement(2095..>2337)
                     /locus_tag="VFQ10_08160"
     CDS             complement(2095..>2337)
                     /locus_tag="VFQ10_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965354.1"
                     /translation="VKTPMVMPTDEMAVAAALTMCAGVDPAEARLVRIENTLKLGRIW
                     VSETLLRDAENNERLEILEEPQPIRFEGGEIIPRHA"
BASE COUNT          462 a          718 c          744 g          413 t
ORIGIN      
        1 gtgcggcacg atctccaggg tcaggctcta ccggcggagg cacgagccgg gcgagtagcg
       61 cggagtctcc gagcgccggg tggaccgagc cgggatacat aaggtagtgt aggatctgcg
      121 cttatgtgcg ccgtagtccg caggagcccc tcggtacagt cggtcgaccg agccgtctcc
      181 gttatggagt tcctgtcgcg ccgcgggtgg tcgggcgtga ctgaggtcgc cagggagctc
      241 gacatccaca aatccaccgc gtaccggcta ctcacgacgt tgagagagcg gggactggtc
      301 gagcaggacg cggcgaccga gaagtacagg ctgggcttcg ggttggtgct actcgcgagg
      361 gcggtgaggg ccgacctcga tatccttggc tgcgccaggc ccgtctgcga gcgtttgagc
      421 gagcgtacca gggagacggt caccatcgct gtcctggagg acgatgacgc cgtcgtcatt
      481 caccagagca tctctcgggc gtccgccctc ggcgttgact ggacgggaca tcacacgccg
      541 ttgcacgcca cggccgcggg gaagatcttt ctggcctaca tgccagaaga tcaactcctg
      601 cgcatcctgg aaggaccgct ggagcgcttt acggagaaca caatcgctga cccggcaagc
      661 ctgaaagacc acacgagcaa gattcgggac gaaggctacg gctataccgt cgaggaactt
      721 gagccggggt tgaacgcggt cggagcgccg attcgccgtg ccgacggcgc ggtcgatggt
      781 gcggtgagcg tgtctggccc ggccttccgt ctgccacccg atgctctccc cgagatcggc
      841 gaactatcca aacgggccgc caccgagatc tcccagtgcc ttgggttcca gggctgaacc
      901 gagcagcgga ggctctcgac aaccggaaag gggacgacgt gagtggttac gaagatttcg
      961 ggagcagcct gcgcgatctc ccgcgcctga gaagcagccg ccgcagacgg gcatcgagtt
     1021 gggaccgctc cggcggcaac gacgaccgga tacacgtgga gcccggtgcg accaagaccc
     1081 tggccgaaat gggcggcgcc ggttccataa atcatgtctg gatcaccgtc gccaacgagc
     1141 acatggacag gaccccgaac gctatcgagc ctgatttcct gcgcaggctg ctcctcagga
     1201 tgtattggga tggagaggaa gaaccgagtg tgctcgtgcc cttgggggat ttcttcggcg
     1261 tgggccacgc ccgcacgacg aactttcact cggcaccctt gcagatgagc cccgggaatg
     1321 gcaagtcctt caactgcttt ttccacatgc ctttcgccga gggcgccagg atagacctcg
     1381 tgagcgagct ggagcacgag aatgtcctct tctattacta cgtggattac gaggagctcg
     1441 acacgctcga gcagggtctg ggacgcttcc acgcccagtg gcgcagggag aatcccactg
     1501 acggcgtcga tgaagatggc cagagcaacg aggagttcct cttcggcggc gagaacgtca
     1561 cgggcgaggg caactacacc atcatggagg ccgaggggcg cggccaatac gtcggctgcg
     1621 tgctcaacat ccacaacctc cgcgatacca accattggaa ctggtacggg gagggggacg
     1681 acatgatctt tatagatggc gatgaatggc caccctccct gcatgggaca ggcaccgaag
     1741 actacttcaa cacagcctgg tgcccacaag agaagtactc agcgccctac cacggtatca
     1801 ccctcggggg tggggacaac tggggcggtc acgtaagcct ctaccggttc cacgtggagg
     1861 accccgtgac gttcgaacgc tccatcaggg tcaccatcga gcacggccac gccaacaagc
     1921 gctcagatga ctactcatcg gtcgcctact ggtaccaggc cgaaccgcac agacccttct
     1981 ccatactccc cgtcgaacag cgcgtgcccc gttagcgctg atctctacgg accgtaagta
     2041 caggcatcag acttcagatt tcaggtttgc acggttcacg cgcaaacctg actgctatgc
     2101 gtgcctgggt atgatctcgc ctccttcgaa acgtattggc tggggttctt ccaggatctc
     2161 cagccgctcg ttgttctcgg catcgcgaag cagtgtctcc gacacccaga tcctcccgag
     2221 cttgagggtg ttctctatgc ggacgagcct ggcctcggcg gggtcgacgc cggcgcacat
     2281 ggtgagggca gcggcgaccg ccatctcatc cgttggcatg accatgggcg tcttgac
//
LOCUS       DASTKM010000199        16706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_584187, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000199 DASTKM010000000
VERSION     DASTKM010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16706
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584187"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..821
                     /locus_tag="VFQ10_08165"
     CDS             57..821
                     /locus_tag="VFQ10_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9965355.1"
                     /translation="MNVFGIDIGGSGIKGAPVDTQSGELLEERVRIKTPKPATPEAIV
                     GTAVEVVREAGWQGPVGCGFPAVVKGGIVQTAANIDKAAIGFDLRAALLRELDAPVSV
                     VNDADAAGLAEMRWGAGRDQDGVVLMLTLGTGIGTSLFVGGRLIPNTEFGHIEVRGKD
                     AEHRAADSARKRDDLSWEEYAGRLDEYLHRVEFLLWPDLIVIGGGISKKSEKFFPHLT
                     ARTKVVPAEMHNDAGIAGAALAGVPDRAAVQTAEKR"
     gene            complement(872..1459)
                     /gene="folE"
                     /locus_tag="VFQ10_08170"
     CDS             complement(872..1459)
                     /gene="folE"
                     /locus_tag="VFQ10_08170"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET9965356.1"
                     /translation="MSEERSIDGPRIERAVREILLAIGEDPGREGLVETPERVAEAYA
                     HLFSGLDKDPASYLEVSFAADSHDAVLLRDLPLASMCEHHLLPFVGRAHVAYVPEERV
                     VGLSEIVHLVEGFARRPQLQERLTAQVADALYRTLGSRGSVVVIEAEQLCMTMRGAQK
                     PGTVTVTSAVRGVLDEDPGRRAEIMALISRSERRG"
     gene            complement(1642..1863)
                     /locus_tag="VFQ10_08175"
     CDS             complement(1642..1863)
                     /locus_tag="VFQ10_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965357.1"
                     /translation="MLRVGNVRDEAAMESVRDALDRLGVDYEHVRSEPDDDRFPQTAY
                     FYVPDDSAEDVEWVLADLSGEHGFDAEVL"
     gene            complement(1868..2986)
                     /locus_tag="VFQ10_08180"
     CDS             complement(1868..2986)
                     /locus_tag="VFQ10_08180"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET9965358.1"
                     /translation="MVDERVGKLARILVDYSIEAGEGDQVLVSAEVGAGPLIGALYAR
                     LLQVGATAVTQISLPGMQELFFEHAQELHYQEIPQVTRAIYEGVDARIGIRAPSNTRA
                     LANVDPEKQQALQKRNKPLSEMMLEKDRWVLMLFPTEALAQEAHMSLSEYEEFAFEAM
                     GLNEEDPVRYWSQMSAEQERLKSRLEEAREIRIVGPETDLTLSVEGRTFVNSAGMRNM
                     PCGEVFTGPIEDSANGTVYFGVPAAIAGREISGVRLRFEEGKVVEASAEKGEEYLSSL
                     LDADAGARYLGELGIGTNYGIRQASANVLFDEKLGGTVHLAIGRSYAQTGGKNDSSVH
                     TDLVCDLREGGALYADGELIQENGHFLEFDLAGVTDAR"
     gene            complement(3098..3877)
                     /locus_tag="VFQ10_08185"
     CDS             complement(3098..3877)
                     /locus_tag="VFQ10_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET9965359.1"
                     /translation="MAGPVIFSTGSLYPFGLDRVYAWISEAGYDGVEIMMDERWDTHQ
                     EHYLRDLVERHGVPILALHPPLRRGAWELTPEETLARVARLASRLDVPVVVAHPPPPG
                     RPLERWKAGPLCEAREQGVVVAVENMPKSRPGGLFRVGRKSCYLPEHLAGVGEVTLDT
                     SHAGASKVDPLRAHSVLATQLRHVHLSDSNLELGRDEHRLPGRGRLPLKPFLAALGAS
                     GYPGAVSLELKPWPLGTPDPEAILARMHAALRFTREGLGAI"
     gene            complement(3877..4689)
                     /locus_tag="VFQ10_08190"
     CDS             complement(3877..4689)
                     /locus_tag="VFQ10_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9965360.1"
                     /translation="MDDSAQQRLFTETPGSIEPPEKGLYLGTSGWSYADWEGTLYPEA
                     LPSASRLAEYAKHFATVEIDSTFYGTPRRSTVEKWREIVPDGFHFAAKFPQEVTHERN
                     LVGSEAESESFVRTMAELGDRLGPLLLQLPPSFDVEGMGVLEDFLKRLPEGFRYAVEV
                     RHRSWLGSDLPEMLRERGAALALIDYPRMPRMEQATADFSYIRWLGDRREFPSGHTHL
                     KKERDDDLRWWSDLVGRFLQEGRTVFAYANNHYQNHSPSTLDRFLEIRRGEI"
     gene            4807..5742
                     /locus_tag="VFQ10_08195"
     CDS             4807..5742
                     /locus_tag="VFQ10_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET9965361.1"
                     /translation="MKVFYSDHFVLPLPEDHRFPMVKYSMLRERVARDGICGPGELST
                     PRAVTDEEILRAHAPDYLKRVASGTLTEKEVRRIGFPWSEKMVERSRRASGGTLAACL
                     AALDEGFAANLAGGTHHALSDRGEGYCVLNDSAIAARAVQAAGLAERVVVIDTDVHQG
                     NGTAAILHGDPTVFTFSIHGAKNFPFHKEGSDLDTPLPDDAGDTEFLAALERGLEVAL
                     DAADASLAIYLAGADPFEGDRLGRLSVTKSGLAERDRIVLETCRERGIPVALTMAGGY
                     ASEVEDTVDVHFQSIRRAADLLEGAADRPKETHQT"
     gene            5739..6737
                     /locus_tag="VFQ10_08200"
     CDS             5739..6737
                     /locus_tag="VFQ10_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9965362.1"
                     /translation="MSGNQRSCVIVRAGISGLLAANTLKQEGWTVTVLDKGLVGGRMA
                     TRRVGEGTFDHGAQFFTVRGERFAGLVEGWLTAGVATEWTRGFADAEGQPNEDGHPRY
                     RGSEGMASIPKHIAQGLDVRTGERVVGMDEGDDCWEVVCESGLRVAGDALVLGAPVPQ
                     ALKLTSSGGYTLPDGAKRQLEAVSYDPCLALMVLLEGPTGVPEPGGMQIKGEPLDWIG
                     DNKRKGISGAPGITVHAGPKWSRSHFEDGESEVIDSMISFAGDRLGGDLAPAVVEASL
                     ARWRYSWVTNPHPEPCLVASDKPPLLFCGDAFGQPKVEGAALSGLAAADHLLGRNA"
     gene            6940..7287
                     /locus_tag="VFQ10_08205"
     CDS             6940..7287
                     /locus_tag="VFQ10_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965363.1"
                     /translation="MGNRRVALKPHASKIRQWVEEGRGDAWIAQELNTTPSSVQSFRS
                     RNSIYRRDPVRRGQLSEHPAVLEETEGGMVLKTDARDSEVFAREWRHYLHRSPEDLQV
                     VITQDRIYLEKVR"
     gene            7289..7579
                     /locus_tag="VFQ10_08210"
     CDS             7289..7579
                     /locus_tag="VFQ10_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965364.1"
                     /translation="MHRQPAVELRIPLEEAEALHALLERLLEGGEQDPRLEHSYRLLG
                     WRILAARGGTGLTGRIADLAREADSLEEYEAARERELGPVLDGLERGENRDP"
     gene            7819..8829
                     /locus_tag="VFQ10_08215"
     CDS             7819..8829
                     /locus_tag="VFQ10_08215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9965365.1"
                     /translation="MQYVLSSLATMVFIGILALLAQWSRKSRRAEISLLIVLVFLSLL
                     LFATGALLGVVWVSGQMPPDVYPQQLLAVTAVPVAMSGLIGLALCVPTLLKIIRGRPN
                     GEFWTDPPIFLALWLFATVLLANNLVGILGFEQLNQVDAFSLGTGGRIPPVAILASQL
                     PFVVIAFLGVGAGIRRGPRETLARLGYGPISPMHIGIVVLFIAGAFALSLAADALFSQ
                     LQPDLYRRVGEISQTLFDPKGLSLISAVIFALLIGVGAGLGEETLFRGAVQPVLGIPI
                     TSVLFASMHVQYGPSLLLGYIFVLSIGLGLLRRYVNTTASFLAHAGYNTLGILAVYLF
                     GL"
     gene            complement(8852..9340)
                     /locus_tag="VFQ10_08220"
     CDS             complement(8852..9340)
                     /locus_tag="VFQ10_08220"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9965366.1"
                     /translation="MRFTLKPITPSDARAISRWRYDEPYSIYDGDPNSVDALLQPHFS
                     YHSVYDEHGDLTGYFCFGEDAQVAAGKRLGVYQVEPALDVGLGMRPDLTGRGLGEEFV
                     HAGLGFAREVYSPPAFRLTVAAFNRRAIRVYERAGFETVETFGAPTLGAEREWLLMRC
                     GA"
     gene            complement(9359..9643)
                     /locus_tag="VFQ10_08225"
     CDS             complement(9359..9643)
                     /locus_tag="VFQ10_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9965367.1"
                     /translation="MSPERHAGDLLSTIGETLRAERNERGLTLKQVAEGAHVSVSYLA
                     EIERGEKDPSSRVLESIASGLDIEVSELLVRIASALEPAPVVTVTDSLAA"
     gene            complement(9669..10547)
                     /locus_tag="VFQ10_08230"
     CDS             complement(9669..10547)
                     /locus_tag="VFQ10_08230"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET9965368.1"
                     /translation="MPNRTNRIGRHLPTSGGLARTLWAARELGCETIQIFVSNPQAWT
                     DPRRRQDADIFVEGSLEMGIWPVVAHAKYLINLASTSVEQRDLSVLALANELAAAGAL
                     GVDLVVVHAGSHGGDGEEKGMERLVEGLERARERAAGLEGARGAVAEPVVENSVGAGT
                     QLCSTFEELGELTRRGGVRVCIDTAHAFVAGYDLSAESGGRGVAEELVDAVGDAVALV
                     HLNDAKYELGSHRDGHRKVGEGCITVDAWPGFFDGLAGVPVVMETPYGTPETDSEEVL
                     LVKKLAGGLRKAPIGR"
     gene            10636..11082
                     /locus_tag="VFQ10_08235"
     CDS             10636..11082
                     /locus_tag="VFQ10_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgT C-terminal domain-containing protein"
                     /protein_id="HET9965369.1"
                     /translation="MAVEGKVAKILGNNEIVINRGRGEGVRQGMLFEIFAPEGEEVWD
                     PDTGETLGTVEDVKAKAEVTEVKDRLAVARLQNVQDTPFGAVNIGEMQENLQRMFGQM
                     FGEDVQIRGFGSTSGDDPDLESMLGGPLQDLSKVQVGDAVREIKRR"
     gene            11103..11792
                     /locus_tag="VFQ10_08240"
     CDS             11103..11792
                     /locus_tag="VFQ10_08240"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9965370.1"
                     /translation="MNELSRLPVTYGLIGTCVAVYVAVAFAGQGHGYPLNFGLVTQPP
                     EVLAQGALMPAAVADGRVWLLLSSMFLHSGFIHLALNMLSLYFLGSFVEQAFGKSRFL
                     ALYLSSGLAGGIAYLYFGAFDKPAVGASGAIFGLLGGVLGYSVRRGTFSWQNPLIRQL
                     LILTALNLYFGFSVPNISNTAHIGGLVGGAAFGWLTAPTAHSSKSLRAATPTVILFGA
                     ELILLALWLLL"
     gene            complement(12041..12778)
                     /locus_tag="VFQ10_08245"
     CDS             complement(12041..12778)
                     /locus_tag="VFQ10_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET9965371.1"
                     /translation="MAAVNAILMALLAAVGFSAASLLALVGGDLEARGRSYAASFAAG
                     ILLALAFVDLFPEGLELGGEAAVAGFVGGFALLFMTEVFTRAHTHHLPEEHVPKHALG
                     PFVLGLAVHNLADGFVLGVGAKAGAVTSWLVGLGVLVHQIPVGISLAAVLIAARVGRA
                     GVMRVAVLLGLAIPVAAALTVAFPAPGEGTLGLLTGVAGGVLAYIGAAHLLPEAQAED
                     TNRSAGIMFVATFVITAAGLIALPHFG"
     gene            13080..14912
                     /locus_tag="VFQ10_08250"
     CDS             13080..14912
                     /locus_tag="VFQ10_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9965372.1"
                     /translation="MVYNLVLKGIRIDSQEELQGGFAALALMRSDLLFNLGYVFLWVG
                     LFALTRQSRLRWPVVVLFHAVTILVIATTTSAHGYFQETGSTLSLSVITYSLRSLGEI
                     TDIIGSVTSLAIWAWVLVILGYIVLGPWIITTLVCGKPGEPGSFGSPSTSRLFALGAF
                     TMSLGFIFLSLPVGAGVAGKSFSRDAVVNIVMSEVEHAQIRDLVANASSIEPPVDTRL
                     QKTAQTEKKNVVLIHLESARARSMTPYNEDLDTTPYLDTLSKNSLLAERAYTIVPHTS
                     KAVTSINCGIDPHLVREITEAEPGRVPARCLPELLKEQGYNSVWFSSATEHFEDRRDL
                     VENFGYDDFRPVETMETEGFQKSNYFGYEDDIMLQPSRSWLEQHKDEPFLATYLGVTG
                     HHDYRPIDRYGQKDYSNSPPLDHYQNEMRYLDFFVRNVIDQYKELGLYEDTIFVIYGD
                     HGEGFGEHDLYQHDNTIYQEGLKVPFIIHTPGSFEEGKRVETLTNQLDIPPTLFDMLG
                     YKVKGGHYPGISLLGPPQEDRTLFFSCLDEYRCLASIQGTEKYIYFFGNQPEEVYDLA
                     SDPLERNNIANQQSRKELKQKRLQVLAWYSKVNAMYRERDSGQE"
     gene            complement(14918..15742)
                     /gene="lgt"
                     /locus_tag="VFQ10_08255"
     CDS             complement(14918..15742)
                     /gene="lgt"
                     /locus_tag="VFQ10_08255"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET9965373.1"
                     /translation="MNLLSLPSPPSPVMFEVGPFALRYYGLCIALGIIVATWLTGREL
                     ERKGYDGTLALDSLFYIVPLGFIGARAYHVITDYGLYSDDPFPGVFEVWNGGLGIYGG
                     VVGGFVGLLIFARVRGISPWVFADAVAPGLVLAQAIGRWGNYFNQELFGRPSDLPWAI
                     RIAPENRPVGYSDAAAFHPTFLYESIWDVLVCLILLWVARRFADRLKSGDVFLLYVSL
                     YSFGRFFVEALRVDPAFLIGGTIRGNLFVSSVLALGFAMILLLRHSGSRRKRPESR"
     gene            complement(15744..>16706)
                     /locus_tag="VFQ10_08260"
     CDS             complement(15744..>16706)
                     /locus_tag="VFQ10_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9965374.1"
                     /translation="AYTIVNDTAIMNGTGCWMPSSVSASPCYRSWRQNSPNYQFLGDH
                     GPQYLSEAISDSNDIFFYQVADWIWNQTRDKDWLPHYYERWGFGRITGIDLSGETAGR
                     IPTEQGERELYEALHGSEEGAYWSVGDWVNLAIGQGDLLVSPIQMARAYSALYNGGTL
                     VTPHVGKEITDQSGKVVETISPRTAGRVDTNQYQIDLVIQGLRGVTEKDGTAGAVFVD
                     STLPVVGKSGTGELPAGRDYVNWFAGWAEGQEEPLVVVTMIEGGGSFQEGSEMTAGPA
                     VRHILEAYYGVEQSPKDPHPTGTPPLDPADRASTESESDAAPVE"
BASE COUNT         3078 a         5416 c         5144 g         3068 t
ORIGIN      
        1 tcgggcggga gtgtgggatc gtatagaatg aggacgtatc cgtctctgga gggctcatga
       61 acgtgttcgg gatagatatc ggagggtcgg ggatcaaggg cgcgcccgtg gacacccagt
      121 cgggtgagct gctcgaggag agggtgagga tcaagactcc caaaccggcc acgcccgagg
      181 cgatcgtcgg gacggcggtc gaggtggtca gggaggccgg gtggcagggc cccgttggct
      241 gcggattccc cgccgtcgtc aagggcggca tagtgcagac cgcggcgaac atagacaagg
      301 cggcgatcgg cttcgacctg cgcgcggctc tactgagaga gctcgacgcc cccgtgagcg
      361 ttgtcaacga tgctgacgca gccggtctcg cagagatgcg ctggggcgca ggccgagatc
      421 aggacggcgt cgtcctgatg ctcacgctgg gtactggaat cggcacttcc ctcttcgtcg
      481 gcggccggtt gataccgaac accgagttcg gacacatcga ggtcaggggc aaggacgccg
      541 agcaccgcgc cgcagacagc gcccgcaagc gcgatgacct gagctgggaa gagtacgccg
      601 ggcgcctcga cgagtacctt cacagggtcg agttcctcct ctggccagac ctcatcgtca
      661 tcggcggcgg cataagcaag aagtccgaga agttcttccc ccacctcacg gcgcgtacga
      721 aggtggtgcc ggctgagatg cacaacgacg ccggtatcgc cggggccgca ctggccggag
      781 tccccgatag agcagcggta cagaccgcag aaaagcggta atccgggctc tgacctactc
      841 cggcggtcag gtcatctggc ggggtgctca gctaaccacg ccgctcgcta cgcgagataa
      901 gagccatgat ctctgccctg cgtcctgggt cctcatcgag gacgcccctg accgccgagg
      961 tcaccgtaac ggtgcctggc ttctgggcgc ctctcatggt catgcaaagt tgctcggcct
     1021 cgatgacgac gacggaaccg cgagatccca gggtccgata gagcgcgtcg gcgacctgtg
     1081 cggtgagtcg ctcctggagt tgcggcctgc gggcgaagcc ctcgaccagg tgcactatct
     1141 ccgagagccc aacgacgcgc tcctctggaa cgtacgcgac gtgggccctg cccacaaagg
     1201 ggagcaggtg atgctcgcac atgctcgcaa gcggcaggtc ccgcaacaag accgcgtcgt
     1261 ggctgtctgc tgcgaacgag acctccaggt agctggccgg gtctttgtcc aggcccgaga
     1321 agaggtgggc gtatgcctcg gcgacgcgct cgggggtctc gaccaagccc tcccgccccg
     1381 ggtcctcgcc gatagcgagc aggatctctc gcacggcccg ttcgatgcgt ggtccatcta
     1441 tgcttcgctc ttcggacatc cttctaaccc cagatccacc cacacaagcg tacggtcgac
     1501 gtcaatgaac actgcgcgta gcgcatccta acttcttccc ggccctcatg gcacttttcg
     1561 gacctacgga ccatcaccgt ggtacgaatc cgccttctga tccacgtctg ttttgcagga
     1621 aactcgttct gcacagcacc ttcaaagcac ctcggcgtcg aagccgtgct ccccggagag
     1681 atccgcgagc acccactcta catcctcggc cgaatcgtcg ggtacgtaga agtaggcggt
     1741 ctggggaaag cggtcgtcgt cgggttcgga gcggacgtgc tcgtagtcca cgcctagccg
     1801 gtcgagcgcg tccctgacac tctccatcgc agcctcgtcc ctgacgttcc ccacgcgcag
     1861 catcgcctca cctggcatcg gtcaccccag cgaggtcgaa ctcgagaaag tggccgttct
     1921 cctggatcaa ctctccatca gcgtacagtg ctccgccctc gcgcagatcg cagacgagat
     1981 cggtgtgcac gcttgagtcg ttcttgccac cggtctgggc gtaggagcgg cctattgcca
     2041 ggtgaaccgt cccgccgagc ttctcgtcga agaggacgtt ggcgctcgcc tggcggatgc
     2101 cgtagttggt cccgatcccg agctccccaa ggtagcgggc cccggcgtcg gcgtcgagca
     2161 ggctagaaag gtactcctca cccttctccg cactagcctc gacgaccttc ccctcctcga
     2221 accggagccg cacaccggag atctcgcgtc ccgctatcgc ggcgggtacg ccgaagtaca
     2281 ccgttccgtt tgccgagtcc tctatgggtc cggtaaagac ctccccacac ggcatgttcc
     2341 tcattcccgc cgagttcacg aacgtacgtc cctcgaccga gagcgtcaag tccgtctcgg
     2401 gtccgacgat acggatctcg cgggcctcct ccagccgcga cttcagccgc tcctgctcgg
     2461 cggacatctg gctccaatag cgcacggggt cttcctcgtt gagtcccata gcctcgaacg
     2521 cgaactcctc gtactccgag aggctcatgt gcgcctcttg cgccagggcc tcggtcggga
     2581 agagcataag aacccagcgg tctttctcca gcatcatctc gctcaagggt ttgttgcgct
     2641 tctggagagc ctgttgcttt tcgggatcaa cgttcgcgag cgccctggta ttgctcggcg
     2701 cgcggatgcc gatccgggcg tccacgccct cgtagatggc gcgcgtgacc tgcgggatct
     2761 cctggtagtg aagctcttgt gcatgctcga agaacagctc ctgcatccct ggcaggctaa
     2821 tctgtgttac tgcagtggcg cctacctgca acagccgcgc gtatagagcc ccaatcaggg
     2881 gtcctgcgcc cacctccgca gagacgagga cctggtcccc ttccccagct tcgatagagt
     2941 agtccaccag gatccgtgca agtttgccga ccctttcgtc taccatcact ggctcctttc
     3001 tgaagaagtt cccggagagc ttatcatccg cgtcgtcccc cgaagccttg cacgcagcgc
     3061 gcccaatggt aaggccctgg gtcgggcaag gcactgccta gatggcgccg aggccctcac
     3121 gcgtgaacct cagcgccgcg tgcatcctcg ccagaatagc ctcgggatct ggggtcccta
     3181 gcggccaggg cttgagctcc aggcttacgg ctccgggata accgctcgcc ccgagcgcag
     3241 ccaggaaagg cttcaggggg agccgacccc taccaggcag gcggtgctcg tctcggccca
     3301 actcgaggtt cgagtcggac agatggacat gacggagttg ggtcgctagg accgagtggg
     3361 ccctgagggg gtcgaccttg ctggccccgg cgtggctggt gtccagtgtg acctcgccca
     3421 cgcccgcgag gtgctcgggc aggtaacagc tcttccgccc aaccctgaaa aggcctcctg
     3481 ggcggctctt gggcatgttc tcgacggcca cgacgacgcc ctgctcccgc gcctcgcaca
     3541 gaggtccagc cttccaccgc tccagaggcc tgcctggcgg tggcggatgc gccactacga
     3601 caggcacatc cagcctactg gccagtctcg cgacccgcgc cagcgtttct tcaggagtaa
     3661 gctcccaggc accacgtcgc agcggagggt ggagggccag gatcgggacg ccgtgcctct
     3721 cgacgaggtc tctcagatag tgctcctggt gggtatccca gcgttcgtcc atcatgatct
     3781 cgaccccgtc gtacccggcc tccgagatcc aggcgtatac cctgtcaagg ccgaaagggt
     3841 agagcgagcc cgtactgaag atgacggggc cagccatcag atctctccgc gccggatctc
     3901 caaaaaccgg tcgagcgtcg agggcgagtg gttctggtag tggttgttgg cataggcgaa
     3961 gacggttctt ccctcctgga gaaagcgacc tacgagatcc gaccaccacc tcaggtcgtc
     4021 gtcgcgctct ttcttgaggt gcgtgtgtcc cgaagggaac tcccgccggt cgccgagcca
     4081 cctgatgtac gagaagtcgg cggtcgcttg ctccatccgg ggcatcctcg gatagtcgat
     4141 gagagcgagg gccgcaccac gctcgcgcag catctcgggg agatcagagc caagccagct
     4201 cctgtgacga acctcgacgg cgtagcgaaa tccctcgggc aagcgtttca ggaaatcttc
     4261 gaggacgccc atcccctcga cgtcgaagga tggcgggagc tgaagaagga gcgggccaag
     4321 tctgtctccc agctctgcca tcgtacgcac gaagctttcg gactcggctt cggagccgac
     4381 gaggtttctc tcgtgcgtca cctcctgcgg aaacttcgca gcgaagtgga agccgtccgg
     4441 gacgatctca cgccacttct ccaccgtcga gcggcgcggg gtgccgtaga aggtagagtc
     4501 tatctccacg gtcgcgaagt gcttcgcgta ctcggcgagc ctcgaagcgg aagggagggc
     4561 ttcggggtag agggttcctt cccagtccgc atacgaccag ccggaggtac ccaggtaaag
     4621 gcccttctcc ggcggctcga tgctgccagg agtctccgtg aagagccgct gttgcgcaga
     4681 atcgtccatc taccctcgaa gctgcgatgc taccatacga gcccctgcgg tttttatgaa
     4741 tgctcttcct gttctccccc gccgcatgta ctacattagc gcagtggacg agagacgcca
     4801 gacaccatga aggtcttcta cagcgaccac ttcgtactac ccctgcccga ggaccaccgc
     4861 tttccgatgg tcaagtactc gatgctgcgc gagagggtag cgcgcgatgg gatctgcggc
     4921 ccgggagagc tctcgacgcc acgagcggta acggacgagg agatcctgcg cgcccacgcc
     4981 cccgactacc tgaaacgcgt cgcctcagga acgctcacag aaaaggaggt gcggcgcatc
     5041 ggtttcccgt ggtcagagaa gatggtagag cgctcccgcc gtgcctcggg cggtacgctc
     5101 gcggcctgcc tcgccgccct cgacgagggc ttcgccgcca acctcgctgg agggacgcat
     5161 catgccttat ccgacagggg cgagggatac tgcgtcctca acgactcggc catcgccgcc
     5221 cgcgccgtcc aggccgccgg tctcgcggag cgcgtggtcg tcatcgacac ggacgttcac
     5281 caggggaacg gcacggcggc gatcctgcac ggagacccca ccgtgtttac gttctcgatc
     5341 cacggcgcga agaacttccc cttccacaaa gaggggagcg acctcgacac ccccctcccc
     5401 gacgacgctg gcgacaccga gttcctggcg gcactcgaaa gaggattgga ggtggcgctc
     5461 gacgccgcgg atgcaagtct cgcgatctac ctcgcaggtg ccgacccttt cgagggagac
     5521 cgcctcgggc ggctctccgt aacgaagtca ggcctcgccg agcgcgaccg gatcgtcctc
     5581 gagacctgca gggaacgcgg aatcccagtc gcactaacaa tggcgggcgg ctacgccagc
     5641 gaggtggagg acacggtgga cgtacacttc cagagcatcc gtagggctgc cgatctgctg
     5701 gagggagcag cggatcggcc gaaagagact caccagacgt gagcggaaac cagagatcct
     5761 gcgtcatcgt aagggccggc atctccggtc tgctcgcggc caacaccctg aaacaagaag
     5821 gttggaccgt aacggtcctg gataagggcc tcgtcggcgg taggatggct actaggcggg
     5881 tcggggaagg caccttcgac cacggtgccc agttctttac cgtcaggggc gaacgcttcg
     5941 ccggcctcgt cgaaggctgg ctcacggcag gtgtcgccac agagtggacc cgcggcttcg
     6001 ccgacgcaga gggccagccc aacgaagatg gccatccccg ctacagagga tctgaaggta
     6061 tggcctccat ccccaagcat atcgcccaag gcctcgacgt gcgtaccgga gagagggttg
     6121 tagggatgga tgagggcgac gattgctggg aggtagtgtg cgagtccggc ctgcgggtag
     6181 ccggggacgc tctcgtgctc ggggcccctg ttcctcaggc gttgaagctg acctcgtcag
     6241 gaggatacac gctgccggat ggagccaagc gccagctcga agccgtctcc tacgatcctt
     6301 gcctcgccct catggtcctg ctcgagggtc caaccggcgt gcctgagcct ggaggaatgc
     6361 agatcaaggg agagccgctg gactggatcg gcgacaacaa gcgtaaaggc atttccggag
     6421 cccccggcat cacggtccac gctggaccga aatggagccg ctcgcacttc gaggacggcg
     6481 aatctgaggt catagactcc atgatctcgt tcgctggcga ccggctcggc ggcgacctcg
     6541 cacccgccgt ggtcgaagcc tccctagcca ggtggcgcta cagctgggtt acgaaccccc
     6601 accctgaacc ttgcctcgtc gcctccgata aaccgccact gctgttctgc ggcgacgcct
     6661 tcgggcagcc caaggtcgag ggcgcagccc tctcaggcct cgccgctgca gaccatctcc
     6721 tcggccgcaa cgcctgacac cccgtgtcct tgttgctcct cgcccatctc gcggcgacac
     6781 tgttaagccc ttagccctca gaaaacaata tgctgaccgc caaccgctgc cctctgacag
     6841 gctgatggga cgagaagcgt agggatgcat ctagcgtcgg gtaacaaaaa gtggtacata
     6901 tgacctagaa aacaacacag ggtgaaggag aaggggacat tgggaaacag aagggtggcg
     6961 ctaaagccgc acgcgtccaa gatcaggcag tgggtcgagg agggtcgggg tgacgcgtgg
     7021 atcgcgcaag agctgaacac cacgccctcg agcgtacaga gcttccggtc gcgaaactcc
     7081 atctaccgca gggaccccgt ccggcgcggc caactctcgg agcatcctgc ggtgctcgag
     7141 gagacagaag gtggcatggt cctgaagacc gacgccagag attccgaggt cttcgcccgt
     7201 gagtggcgcc actacctgca tcgctcgcct gaagacctcc aggtggtgat cacgcaggac
     7261 agaatctacc tggagaaagt ccgctagctt gcacagacag cccgccgtcg aactgcggat
     7321 acctctggag gaggcagaag cactgcacgc gcttctggag cgccttctcg aaggcgggga
     7381 acaagacccg cgcctcgagc actcctacag gctcctgggc tggcgcatac tcgccgcgag
     7441 gggcggtaca ggtctcacgg gacgcatagc agacctggcg cgcgaagcgg acagcctcga
     7501 agaatacgag gcagcgcgcg agagggagct gggtcccgtg ctcgacggcc tggagcgcgg
     7561 cgagaaccgg gatccatgaa taggaggtgt caggctacaa gtatctgatg ccttctcaaa
     7621 gcatggccca tgaaacagga gcgcctacac gagcctgaag cccaacttgt tatccgacag
     7681 tgccttaggg tagcggtatt gtaacttcgc tgctcttttt tccggaccct cggctctttt
     7741 caccttccca gaagagcggc cggggccacg gatcatgcgc ggtccggcat tttacatgtg
     7801 ttaacctctg ttggcgttat gcagtatgtc ctcagttcgc tcgcgacgat ggttttcatc
     7861 gggatactcg cgctcctcgc acagtggtcg agaaagagca ggagggccga gatatcgctc
     7921 ttgatcgtgc tcgtgttcct ctcgctcctg ctcttcgcca ccggggcttt gcttggcgta
     7981 gtgtgggtct cgggccagat gccccctgat gtctatccgc aacagctgtt ggccgtcacg
     8041 gcagtacctg ttgcgatgtc gggtttgatc gggctggcac tctgcgtccc cacgctgctg
     8101 aagatcattc gcggcaggcc gaacggggag ttctggacgg acccacctat ctttctcgcc
     8161 ctctggttgt tcgccactgt gttgcttgcc aataaccttg taggcatact cgggttcgag
     8221 cagttgaatc aggtggacgc cttctctttg gggaccggcg gtaggatccc accagtcgcc
     8281 atcctggcca gccagttgcc cttcgtcgtc atcgcctttc tgggggtcgg cgccggcatc
     8341 cgccgcggac cgcgcgagac gctcgcccgc ctcggctacg gtcccatctc cccgatgcac
     8401 ataggtattg tcgtactgtt tatcgcgggt gcgttcgcgc tctcgctagc cgccgacgcc
     8461 ctgttctcgc agttgcagcc tgacctttat cgcagggtcg gtgagatctc gcagacgctc
     8521 ttcgacccga aaggtctgag cctgatctcg gcggtgatct tcgccttgct gatcggggtg
     8581 ggcgccggac tcggagagga gacgctcttc cgcggcgccg tgcagccggt cttggggata
     8641 ccgataactt ccgtcctttt cgcctcgatg cacgtccagt acggtccatc gctgctgctc
     8701 ggctacatct tcgtgctctc cataggcctc ggactcctcc gccgctacgt caacaccacg
     8761 gcgagcttcc tggcgcatgc aggctacaac acactcggca tcctggccgt ctaccttttc
     8821 ggcctgtaga gaacggccgc aggcggccag cctatgcccc gcatctcatc agcagccatt
     8881 ccctttcggc gcccagcgtt ggagccccga acgtctctac ggtctcgaat ccggcccttt
     8941 catagacgcg gattgcgcgg cggttgaagg cagccacggt caggcggaaa gccgggggtg
     9001 agtagacctc ccgtgcgaag ccgaggccgg cgtgcacgaa ctcttctccc aggccccttc
     9061 ccgtgagatc cggcctcatc ccgagcccca catcgagcgc gggctcgacc tggtagaccc
     9121 cgagccgctt tccggccgcg acctgcgcgt cctcgccgaa gcagaagtat cccgtcaaat
     9181 ctccgtgttc gtcgtagacg gagtggtagg agaagtgcgg ttgcaggagc gcatcaacgg
     9241 agttgggatc tccatcgtag atcgaatagg gttcgtcgta acgccaacgc gagatggctc
     9301 gtgcgtcgct gggcgtgatc ggcttcaagg tgaagcgcaa cgtcggtttc ctgggagtct
     9361 aggccgccag agagtcggtg acagtgacga ccggcgcggg ctcgagcgcc gaggcgatcc
     9421 ggacgagtag ctcgctgacc tcgatgtcca atccactggc gatgctctcc agtaccctcg
     9481 aggaggggtc cttctcgccg cgctctatct cggcgaggta cgagaccgag acgtgcgcgc
     9541 cttcggcgac ctgctttagc gtcagccccc gctcgttgcg ttcagcccgc agggtctcgc
     9601 cgatggtgga gagaaggtct cctgcgtgcc gttccggact catctccaaa gtcttcacgc
     9661 cctagattct atcttccaat cggcgctttg cgcaacccac ccgccaactt cttgaccaga
     9721 agcacctctt cggagtcagt ctcaggggtc ccgtacgggg tctccatgac cacggggact
     9781 cctgccagac cgtcgaaaaa cccaggccag gcgtcgacgg ttatgcaccc ctcccccact
     9841 ttacgatgcc cgtcgcggtg gctcccgagt tcgtacttcg cgtcgttcag gtgaaccagc
     9901 gcgaccgcgt ctcccacagc gtccacgagc tcctccgcga ccccccgccc tccgctttcc
     9961 gccgaaaggt cgtagccggc cacgaaggcg tgggccgtgt cgatgcagac cctcactccg
    10021 ccgcgccgcg tgagctcccc gagttcctcg aacgtcgagc agagctgggt accggccccc
    10081 acggagttct cgaccacagg ttcggccaca gctcctcgag ctccttcgag tccggcggcc
    10141 cgctccctgg cgcgctccag gccttccacg agccgctcca tgcccttctc ctcgccgtct
    10201 cccccgtggc taccagcgtg cacgacgacc aggtcgaccc cgagcgcacc tgcggcggcc
    10261 agctcgttgg ccagggccag caccgaaaga tcccgctgct ccacgctcgt ggaggcaagg
    10321 tttataaggt acttcgcgtg tgcgacgacc ggccagatgc ccatctctag gcttccctcg
    10381 acgaagatat cggcgtcctg gcgtcgcctg gggtcagtcc aggcttgcgg gttggagacg
    10441 aagatctgga tagtctcgca cccgagctcg cgcgcggccc aaagggtcct ggcgagcccg
    10501 cctgaggtcg gaaggtgccg tcctatcctg ttcgtcctat ttggcatggc ggcgattcta
    10561 ccgtcctgag gcggccctcg ccaggagaga tataatctcg tgagagcatc aggattattg
    10621 gaaggagcca atcacatggc ggtggaaggc aaggtagcga agattctggg aaacaacgag
    10681 atcgtcataa accggggaag aggagagggc gtccgtcagg gcatgctctt cgagatcttc
    10741 gcgcccgagg gcgaggaggt ttgggatcct gatacgggcg agacgctcgg cacagtcgag
    10801 gacgtaaagg cgaaggccga ggttacagag gtcaaggaca ggctcgccgt tgcccgacta
    10861 cagaacgtgc aggatacgcc gttcggggcg gtcaacatcg gtgagatgca ggagaacttg
    10921 cagcgtatgt tcgggcagat gttcggcgaa gacgtccaga tccgcggctt cggctctacg
    10981 tcgggcgacg atcccgacct cgagtcaatg ctcggcggcc ccctgcagga cctctcgaag
    11041 gtgcaggtcg gggacgccgt gcgcgagatc aaacgccgct agaccccacg taagatgcgg
    11101 cattgaacga gctctcccgg ctaccggtga cgtacggcct cataggcacg tgtgtggccg
    11161 tgtacgtcgc cgtcgcgttc gcgggacagg gccacggtta cccgctgaac ttcgggctgg
    11221 tgacacagcc ccctgaagtg ctcgcgcagg gcgccctcat gccagcggcc gtcgccgatg
    11281 gcagagtgtg gctcttgctg tcgagcatgt tcctgcactc ggggttcatc cacctggcgc
    11341 tgaacatgct ctctctgtac tttctcggct cgttcgttga gcaggcgttc ggaaagagcc
    11401 gattcctcgc gctctacctg tccagtgggc tcgccggggg catcgcctac ctctacttcg
    11461 gcgccttcga caagcccgcc gtaggggctt ctggcgcgat cttcggtctc ctcggaggcg
    11521 tgctcggtta ctctgtgcgc cgcggcacct tctcctggca aaatcccctg atccgccagc
    11581 tcctgatcct gaccgccctg aacctctact tcggattctc cgtccccaat atcagcaaca
    11641 cggcgcatat cggcggcctc gtgggcgggg ccgcattcgg atggctcaca gcgcccacag
    11701 cccactccag caagtcgctc cgcgcggcga caccgaccgt catcctgttc ggcgcggaac
    11761 tgatcctgct ggccctgtgg ctgcttttgt agcttgtcag cacggcgctt cgcgccttct
    11821 cagcacgctt cgagcctacg gctctcgctt gtcagcattt cagcttctcc ttctccggcc
    11881 tcgcgcagca gcgtatgagg aaccgctgct tacggcggta ggttctacgc ttgtgggcag
    11941 gctcccagac gactagctga aatgctgaga agccgccgag cgcagcgagg cggcgggctg
    12001 acaagcgaag gccaagggcc tgagcgtgct gactagctgg ctagccgaaa tgcggaagtg
    12061 ctatgagtcc cgcggcggtg atgacgaacg tggccacgaa catgatgccg gccgacctgt
    12121 tcgtatcctc ggcctgggcc tcggggagca ggtgtgcggc gccgatgtat gcgagcaccc
    12181 cgcctgccac accggtcagg agaccgagag tgccttcgcc tggcgcgggg aaggccacgg
    12241 tcaacgccgc tgcgacaggg attgccagtc caagtaagac cgccactctc ataacccctg
    12301 cccttccgac acgagccgcg atcaggacgg cggccaggga gatcccaacg ggtatctggt
    12361 gaaccagaac cccgagcccg acgagccagg aggtgacggc tccagccttg gcgcctacgc
    12421 cgagaacgaa tccgtcggcg aggttgtgga cggccaatcc caggacgaaa ggccccagcg
    12481 cgtgtttggg gacgtgctcc tcgggcaggt ggtgcgtgtg ggccctggtg aacacctcgg
    12541 tcatgaacaa gagggcgaac cctccaacga acccggcgac cgcggcctct ccaccgagct
    12601 cgagaccctc ggggaagagg tcgacgaacg cgagcgcgag cagtatccca gcggcgaagc
    12661 tggcggcgta agaccggccc ctggcttcca aatcgccacc cactagagcg agaagactcg
    12721 ccgccgaaaa gcccactgct gcaagcaaag ccatcaggat cgcgttcaca gccgccacca
    12781 gccatcggcc gtggcgccgt gcaaccctac cttacgcccc tctacctgca gtcggagcac
    12841 gtcccgtata cctccaggct gcgggcacga acatctgcaa gatagcaggg ggcttcttgc
    12901 gcgtcaaagc tgcccggttg ggaaattgtg ggtcggctgt caagttctgc gaaatctttg
    12961 taaactctct cgaactcgtg gcttccggat atcacaagcg tctggctcta ttcgcctcct
    13021 tccgctccat tttgagcggt cgggactggg tctacctttc cggtttgctg gttcctctgg
    13081 tggtctacaa tctggtgttg aaggggatcc ggatagactc tcaggaagaa cttcagggag
    13141 ggttcgccgc cttagccttg atgcgttcgg acctgctttt caacctcggg tacgttttcc
    13201 tctgggtcgg gctattcgcc ctgacgcggc agagtcgtct ccgctggcct gtggttgtcc
    13261 tcttccatgc ggtcaccata ctcgtcattg cgacgacgac ctcggcccat ggatacttcc
    13321 aggagaccgg ctcgacgctc agcctcagcg tcatcacgta ctcactccgc tcgctgggag
    13381 agatcacgga cataatcggc agcgtgactt cgctggctat ctgggcctgg gttctggtca
    13441 tcctcggcta tatagtgctt ggtccctgga taataacgac gctcgtctgt ggaaagcctg
    13501 gggagcctgg atccttcggt tctccgtcaa catcaaggct gttcgccctc ggggcgttca
    13561 ctatgtcgtt aggattcatc ttcctctcgc tgccagtcgg tgctggggtg gccggaaaat
    13621 cattctccag ggacgccgtc gtcaacattg tcatgtcgga ggtggagcac gcgcagatca
    13681 gagaccttgt cgcgaacgcc tcttccatag agcctcctgt ggatacccgc ctgcagaaga
    13741 ccgcccagac tgagaagaag aacgtcgtcc tgatccacct cgagtctgcc agggcgcgct
    13801 ccatgacgcc ctacaacgag gatttggaca cgacgcctta cctagacacg ctctcgaaga
    13861 atagcctcct ggccgagcga gcatacacca tcgtccccca cacctccaag gccgtcacct
    13921 cgataaactg cggcatcgat cctcacctcg tacgcgagat caccgaggcc gagcctggcc
    13981 gcgtcccagc ccggtgcctg cccgaactct tgaaggagca ggggtataac tcagtctggt
    14041 tctcctcggc gaccgagcac ttcgaggacc gcagggatct ggtggagaac ttcggctacg
    14101 acgacttccg accggtcgag accatggaga cagagggctt ccagaaatcc aactactttg
    14161 gctatgagga cgacatcatg ctccagccaa gcagaagttg gttggagcaa cacaaagacg
    14221 aacctttcct tgcgacctac ctgggggtca cgggacacca cgactacagg cccatagacc
    14281 gttacgggca aaaggactac tcgaactccc ccccgctcga ccactaccag aacgagatgc
    14341 gttacctcga cttcttcgtt aggaacgtga tcgaccagta caaagagctg gggctgtacg
    14401 aggacacgat cttcgtgatc tacggcgacc acggggaggg attcggggag cacgacctct
    14461 accagcacga caacaccatc taccaggagg ggctcaaggt acccttcatc atccacacgc
    14521 ctggaagctt cgaggaaggc aaacgcgtcg agaccctgac gaaccagctc gacatcccac
    14581 ccactttgtt cgatatgctc ggctacaagg tcaaaggcgg ccattacccc gggatatccc
    14641 tcctcggtcc gccgcaggag gaccgcaccc tgttcttttc ttgtctcgac gagtacaggt
    14701 gcctcgccag catccagggg acagagaagt atatctactt cttcggcaac cagcctgagg
    14761 aggtctacga tctggcgtcg gatcctctgg agaggaacaa catcgcgaac cagcagagca
    14821 gaaaagagct aaagcaaaaa cgtctacagg tgctcgcctg gtactcgaag gtcaacgcca
    14881 tgtacagaga gcgagactcc ggccaagagt agccccgtca gcgggactca ggacgtttgc
    14941 gcctggaccc agagtgcctc aggagaagga tcatggcgaa gcccagtgcc aggacgctgc
    15001 taacgaagag gtttccgcgg atggtcccgc cgatgaggaa agccgggtcg acgcggaggg
    15061 cctcaacaaa gaagcgtcca aaagagtaga ggctcacgta gagcaggaag acatcgccgc
    15121 tcttcagacg gtccgcgaag cggcgggcca cccacagcag tatcaggcag actaggacgt
    15181 cccagatcga ctcgtagagg aaggtcgggt gaaaggccgc ggcgtctgag tacccgactg
    15241 gcctgttttc cggcgctatc cggatggccc agggcaaatc cgaagggcgt ccgaagagct
    15301 cctggttgaa gtaattgccc cagcgcccga tcgcctgggc gaggaccagt ccgggcgcga
    15361 cggcgtcggc gaacacccac gggcttatgc cccggactcg ggcgaagatc aggagcccca
    15421 caaaccctcc gacgacgccc ccgtagatgc ccaatccccc gttccagact tcgaatacgc
    15481 cggggaacgg gtcgtccgag tagagcccgt agtctgtgat aacgtgatag gccctggcgc
    15541 cgataaagcc aaggggcacg atatagaaca gcgagtcgag ggccagggtg ccgtcgtagc
    15601 ccttgcgttc cagttccctt cctgtaagcc aggtcgcgac gatgatgccg agcgctatgc
    15661 acagcccgta gtagcggagg gcgaagggcc ccacctcgaa cattacaggg gaaggcgggc
    15721 tcggcagcga gagaaggttc acgctactct accggggctg cgtccgattc gctctccgtg
    15781 gaagcgcggt ccgcagggtc cagcgggggg gtgcccgtcg ggtgcgggtc cttgggggac
    15841 tgctcgacgc cgtagtaggc ctcgagtatg tggcggacgg caggcccggc cgtcatctcc
    15901 gagccctcct ggaaagagcc gccgccctcg atcatcgtga cgacgaccag cggctcctct
    15961 tgaccctcag cccagccggc gaaccagttc acgtagtccc tcccggctgg cagctcgcct
    16021 gtgccgctct tcccgacgac cgggagtgtc gagtccacga agaccgctcc cgcggtgccg
    16081 tccttctccg tcacgcccct caggccctgt atcacgaggt cgatctggta ctggttcgtg
    16141 tctacccgtc cggcggtcct gggggagatc gtctccacca ctttcccgct ctggtcagtg
    16201 atctccttgc cgacgtgcgg ggtgacgagg gtgccgccgt tgtagagcgc cgaataggcc
    16261 cgggccatct gtatcgggga gacgagcagg tcgccctggc cgatggcgag gttcacccag
    16321 tcccccacgc tccagtaagc cccctcctcg ctgccatgca gcgcctcgta cagctctctc
    16381 tcgccctgct cggtagggat gcggcccgcg gtctcgccgc tgaggtcgat gccggtgatc
    16441 ctaccgaacc cccaccgctc gtagtagtgc ggtagccagt ccttatccct agtctggttc
    16501 cagatccagt cggcgacctg gtagaagaag atgtcgttgg agtcggagat ggcctcggag
    16561 aggtactggg ggccatggtc gccgaggaac tggtagttgg ggctgttctg ccgccagctc
    16621 ctgtagcacg ggctcgccga gacgctcgac ggcatccagc agcccgtccc gttcatgata
    16681 gccgtatcgt tgacgatcgt gtaggc
//
LOCUS       DASTKM010000200         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_584206, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000200 DASTKM010000000
VERSION     DASTKM010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584206"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFQ10_08265"
     CDS             <1..287
                     /locus_tag="VFQ10_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9965375.1"
                     /translation="DEPHAEATTPSGLRVAWDTAELMQEIDPAWKEPSGGHRMSLAFL
                     CSSPEEVDATYERLAALGHAYKEPWDAFWGQRYAIVKDPDGNHIDLFASQ"
     gene            complement(361..1539)
                     /locus_tag="VFQ10_08270"
     CDS             complement(361..1539)
                     /locus_tag="VFQ10_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9965376.1"
                     /translation="MTCTGGRGLRWGAGRAMMQASPIKKWRLVTVADKIVFTRGVPPP
                     EAFPTEELGACFDVAVRENPGVVLQYGQQPGYAPLREELAGEYGVSPQEILIGNGSLQ
                     LQDLVSSYLLKPGAVVYTEQPSYDRAIKTFRRRGARTVGIPLEGDGISVERLEAALGR
                     DVPAFLYLVPDFQNPTGATLSLEKRRRVVELAGEYGFWVIEDIPYRKLRYEGDDVPLL
                     RDIDPSRVITMSSFSKLLSPGIRVGFMISPEDLTRGVTALGEDTYLSPVLPTQAAVAE
                     YLRRGLLGPNVERLKDLYTPRWKAMSGAVRRELPGAQAFIPSGGFFVSVMLPEDANTE
                     NLVARAEDIGLVLTPGAVFFADPEEGDEAEGDRFVRLPFCAVTPEQIEEGVERLASLL
                     "
     gene            complement(1608..>2789)
                     /locus_tag="VFQ10_08275"
     CDS             complement(1608..>2789)
                     /locus_tag="VFQ10_08275"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET9965377.1"
                     /translation="CYSFLAFQTAYLKAHYPEAYMAALMSSVMNTKDRVPQYVAEARA
                     MKIQVLPPDVNESGRRFTVVGETIRFGLSAVKNVGDSCVDSIIAAREEGGPFRDIFDF
                     CERVDPKTYNKRTLESLIKCGAFDNVGPSRAAMLEVHAKAVERSTRGLKGAHEDQFSM
                     FDASELAPPRPEFPNVEEDPRRSLEWEKEALGLYVSDHPLRPVLHKLGKHTDTTVSDL
                     DTYRDGAIVWVGGLATSVRTNTTRKGDMMAMLQLDDTRGFAEVMVFPRVYAKCSACVR
                     EDAVLKVKGKVEIKEGIPRIVALEMEELHLEPGADPVYLDAGSFVGHSQKVARAAFEL
                     LLRHPGESPIFLISGDEMLEEKIGDITDSSELHAELKQLLGPRCLSYTSPAEPQMEQV
                     S"
BASE COUNT          512 a          891 c          854 g          532 t
ORIGIN      
        1 aggacgagcc gcacgccgag gcgacgactc cgtcaggctt gcgcgtcgcg tgggataccg
       61 ccgagctcat gcaagagatc gacccggcct ggaaggagcc ttcgggaggg caccgtatgt
      121 ccctggcttt cttgtgttcg agcccagaag aggtcgacgc gacctacgaa agactcgccg
      181 cgctaggtca cgcgtataag gagccgtggg acgctttctg gggacagagg tacgctatcg
      241 tcaaagaccc cgacggcaac cacatcgacc tcttcgcgtc gcaataagca ggtgcagact
      301 gcttgttggg tgccatcagc tcgtcagctt ttcagccttc gcgtgcgcaa ggtctggctg
      361 tcagagcagg cttgcaagac gctccacgcc ctcttctatc tgttctggcg tcacggcgca
      421 gaaaggcagg cgcacgaatc tgtcgccttc tgcttcgtcc ccttcctcag gatctgcgaa
      481 gaagaccgcg ccgggcgtga gcacgagccc tatgtcctct gcccgggcca cgagattctc
      541 ggtattcgcg tcttcgggca gcatcacgct cacgaagaac ccgcccgaag ggatgaacgc
      601 ctgggcaccg ggcagttctc ggcgtaccgc gcctgacatc gccttccacc ggggtgtgta
      661 cagatccttc aaccgctcta cattcggacc gaggagcccg cggcgcaggt actcggcgac
      721 ggcggcctgt gtggggagga ctggcgagag gtacgtatcc tcgccaagcg ctgtcacccc
      781 ccttgtaagg tcctcgggtg agatcatgaa cccaaccctt atgccagggc tgaggagctt
      841 gctgaacgag ctcatcgtaa tgacacgcga cgggtctata tcacgcagca gcggaacgtc
      901 atcgccctcg taacgcagct tgcggtaggg gatgtcttcg atcacccaga aaccgtactc
      961 gcccgctagc tccacgaccc tccgccgctt ctccagggag agcgtcgcgc ccgtggggtt
     1021 ctggaagtca ggcacgagat acaggaaggc agggacatcg cgtcccagcg ccgcctcgag
     1081 tcgctcgacg cttattccgt ccccttccaa cgggatcccc acggtgcgcg ccccgcgacg
     1141 tctgaaggtc ttgatcgccc ggtcgtagct aggttgctcc gtgtagacca ccgcaccggg
     1201 cttgagaaga taggaggaga cgaggtcctg caattgcagc gatccgttgc cgatcaggat
     1261 ctcctgcggc gagaccccgt attcgccggc aagctcctcg cgcagcggcg cgtaaccggg
     1321 ctgctggccg tactgcaata cgacgccggg attctccctg accgccacgt cgaagcaagc
     1381 ccccagctct tcggttggga acgcctctgg tggtgggacg ccgcgcgtaa agactatctt
     1441 atctgccacg gtcacaagcc tccacttctt gatcggggaa gcctgcatca tcgcccggcc
     1501 cgcgccccag cgcaaccctc tgccccccgt gcacgtcatg ccctggtagt ctccgggtca
     1561 gggcccgcag aaatcctgaa gacggagaga gccgcaaggc gcgcgtccta agagacctgc
     1621 tccatctgcg gctcggcggg ggatgtatag gagaggcatc tgggtcccag gagttgtttc
     1681 agttcggcgt gcaactcgga ggaatccgtg atgtccccga tcttctcttc aagcatttcg
     1741 tcgccggata tgagaaagat aggactttcc ccaggatgtc gcagcagaag ctcgaacgcc
     1801 gccctggcca ccttctgtga gtggccgacg aacgagccgg cgtcgaggta cacggggtcc
     1861 gccccaggtt cgaggtgaag ctcctccatc tccagtgcca cgatgcgagg gatgccttct
     1921 tttatctcga ccttgccctt gaccttgagg acggcatcct cgcgcacgca cgctgagcat
     1981 ttggcgtaga cccgcgggaa gaccatgacc tcggcgaagc cccgtgtgtc gtcgagctgg
     2041 agcatggcca tcatgtcacc cttgcgcgtt gtgttggtgc gcaccgaggt agctagcccc
     2101 ccaacccaca cgatggcccc atccctgtaa gtgtcgagat ccgagaccgt ggtgtctgtg
     2161 tgcttaccca gcttgtgcaa gacgggacgc aaggggtgat ccgagacgta gaggccaagg
     2221 gcctctttct cccactccag gctcctacga gggtcttcct cgacgttggg gaactcgggc
     2281 cttggcggcg cgagctcgga ggcatcgaac atcgagaact ggtcctcgtg cgcgcctttg
     2341 aggcccctgg tagaccgttc gacagcctta gcatggacct cgagcatggc ggcacgcgag
     2401 ggccctacgt tatcgaaggc gccgcacttg atcagcgact cgagcgtgcg cttgttgtag
     2461 gtcttcgggt cgacgcgttc gcagaagtcg aagatatctc gaaacggacc gccctcctcc
     2521 ctggcggcga tgatcgaatc tacgcaagag tccccgacgt tcttgacggc ggagagcccg
     2581 aaacggatgg tctccccgac caccgtaaag cggcgcccgc tctcgtttac gtcgggcggg
     2641 aggacctgta tcttcatcgc cctggcctca gcgacgtact ggggcaccct gtccttggtg
     2701 ttcatcacgc tgctcatgag ggccgccata tacgcctcgg gatagtgggc cttgaggtat
     2761 gcggtctgga aggccaggaa cgagtagca
//
LOCUS       DASTKM010000201        25466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1114515, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000201 DASTKM010000000
VERSION     DASTKM010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 25466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25466
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114515"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /gene="merB"
                     /locus_tag="VFQ10_08280"
     CDS             <1..548
                     /gene="merB"
                     /locus_tag="VFQ10_08280"
                     /inference="COORDINATES: protein motif:HMM:NF009710.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkylmercury lyase MerB"
                     /protein_id="HET9965378.1"
                     /translation="KPASPEEIAAISAKSPEEVRAALDRFPSAEWDEQGRVVGLGLTL
                     QLTPHRLELEGRILFAWCALDALLFPILLGRPASIESPCRGTGELVHIEVTSVGIEAV
                     EPPSAVVSIVAARDLATFRRVSCNNAHFFSSPEAASCWLEKHPEATILSVEDTFRLGR
                     LIAEDLSDISRSAEPADAGRE"
     gene            686..2323
                     /locus_tag="VFQ10_08285"
     CDS             686..2323
                     /locus_tag="VFQ10_08285"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9965379.1"
                     /translation="MIEPKNFRRPKGAAKHRWIGVVVALVLGAFAAISLLTLEPPKPL
                     PASAQASEFSAERAFSHVEQIAERPHPVGSTANAEVRDYLVGQLEDLGLRPTVLEATS
                     ARTKEGTASIARVHNIHARIPGSSPTGHVVLVAHYDSVPRGPGAADDGAGVAAILEIA
                     RAITSSSPPRNDIDIVFTDAEEPWLLGAQAFVREGRLDPQRSVVLNLEAGGTSGPALM
                     FESSLDNATLIPALASAQRPIAGSGWEACYQLLPNDTDFTVFRDAGFAGMNFSLVEGS
                     ARYHTPEDSPANLDPASLQHMGATVLATAHHFAGQGLGVPRGGSLTYFTVLGELVHYP
                     QQLAVPLAVLAAVVFVATLLYARKRGARGVGWAAVSFLGLLVVTTALGIGVWQVLRLL
                     HPGYGSFHTGDTYRPEWYAAGLLALTAALTVAWHLLMRRRHAPEELALATWAWFVVLA
                     ILTAAFVPGAAYLFSWTPLVGSLLLAAAFRWSGEEPTWRYLALSAGAIPAVALLGPLV
                     DLFFFLGLAFASGPMFIAVLALATALPVLDLLALRHA"
     gene            2333..3316
                     /locus_tag="VFQ10_08290"
     CDS             2333..3316
                     /locus_tag="VFQ10_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965380.1"
                     /translation="MAATVVELALFATGLSIDTFDDEHPRQTRLVYALDADQEQAWRY
                     LALSAAAVPAVVLLVPLVDLIFSLGLAFASGPLFITVLVLVTALPVLDLLALRHAWLV
                     PVAAAMVGLVLITAGLRIDTFDDEHPRQTRLVYALDADLERAWWLSADPQPAPWTDRY
                     VDTERIEDDDRFPDSPSLSLSSRYYSGPASVARIEPPDVTVRRSDRDGDTRVLRLRVT
                     PEEGSRIALYADTRSHTVTAATVDGISVEEAPGQRQDTDPSKWGFVFHTVPPEGIDVT
                     LEVRGEGLLPLRVIGYRDGLPQVPELTPRPDDLTWSGTSSNLTMVAESYRV"
     gene            complement(4108..5079)
                     /locus_tag="VFQ10_08295"
     CDS             complement(4108..5079)
                     /locus_tag="VFQ10_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9965381.1"
                     /translation="MKAIVRDTYGSPEVLELRDIDIPEYADEEVLVHVHAAGVGRDVW
                     HIMKGLPYPIRLAGYGFRAPKNPVIGSDMAGVVEAVGKNVTRFQPGDEVFGIGKGSYA
                     EYVCAREDKLAPKPTNLTFEQAAVVAIMGSTALQALRDHGKVSPGQEVLLIGASGGVG
                     TYAVQIAKAFGANVTGVCSTKKVEMVRSIGADHVIDYTREDFAEGDQRYDLILDIGGN
                     STLSRLRRALASRGTLVIVGGEGGGRWLGGTDRQLRAIMLSPFVGQKLGTFVNKENHE
                     DMLVLKELIEAGKVTPIIDRTYPLAEVPEAIRYLEEGHARGKVVISV"
     gene            complement(5419..5931)
                     /locus_tag="VFQ10_08300"
     CDS             complement(5419..5931)
                     /locus_tag="VFQ10_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965382.1"
                     /translation="MATFKLSRPPGVPKEVQASFLLWLTAVAAGVLETIIRVIDASAI
                     GFASGSGGETDVTGAISGVVIRVIVYALVVYIITRMRLGKRWARLTLAVLLGVIGTLS
                     LVIDPISWLAGGNSLSEVFTEANLLFFLIAPIRSVHLAAVIAALALMFLPAANTYFRT
                     SRSVRRQALG"
     gene            complement(6863..7009)
                     /locus_tag="VFQ10_08305"
     CDS             complement(6863..7009)
                     /locus_tag="VFQ10_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965383.1"
                     /translation="MNAKKLMVGLVAAVTLTLFTASVYAQQAAPPQEDDAAEAYFDDR
                     EEFD"
     gene            complement(7196..7753)
                     /locus_tag="VFQ10_08310"
     CDS             complement(7196..7753)
                     /locus_tag="VFQ10_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965384.1"
                     /translation="MRRWLLLVLSVALAVSVFAPVAAAQGDDGGFDDKGMDVRGTDDK
                     GFDDRGMHVRGADDKGFDDRGMNNRGVDDNAVASPASSASASTSAMSSASASASDDRG
                     FDDRGRGMDDRGVGDSAAGSPASSASASASPTASPSASASASPSASPTASPTTTASAT
                     PVLPDTSGFPPAWVLGPLAMILGGG"
     gene            8054..8437
                     /locus_tag="VFQ10_08315"
     CDS             8054..8437
                     /locus_tag="VFQ10_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965385.1"
                     /translation="MIGEPPSLSGSLSGNSSVLVAAVLAALVVAWAATNLVLGGEGFA
                     VLVSDKVVGGEEAASALARLFAALVLGLFLADAVGWRARWVAGGLVVLGLGHFVFGYL
                     EPLIQEDPPELNESLYEAFVTQTLE"
     gene            8447..9646
                     /locus_tag="VFQ10_08320"
     CDS             8447..9646
                     /locus_tag="VFQ10_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9965386.1"
                     /translation="MVGLFPGRPPRLVVWAATAIPIALVAGYVVLFEFLHAEEWMPLL
                     SRVANPERTVTLGSPLVCLTPWHWALSALPLGLASAAVVGAFWQSRRGLLRSWLLIAV
                     VLLAGSVLHDYLWPSAYGGGLLTTADALSLVFAVVVAIGGVSELRRVASERARLLATE
                     RERARRLNELNSLRSDFSAMIAHELDTPIAAVRKLNEMLSAQGEDPGVRDYATTATER
                     ELDALTNLVRDVRAVAAVEREGFGIKARPLPLEELLADAEVYASTLPGHHPIEVMVRG
                     DLRVEVCVLADPERIGQVLRNLLSNAAKYSPEGTPIELRVIGKEGQVRVEIADQGWGI
                     HPDDVPRIFEKFGRGRDRKGHHERPGVGLGLYLSQRIVRGHGSELTVQTRPGEGSVFG
                     FELRVER"
     gene            9643..10311
                     /locus_tag="VFQ10_08325"
     CDS             9643..10311
                     /locus_tag="VFQ10_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9965387.1"
                     /translation="MNREIRIMVVDDHDTFRDPLAFMLEREPDLTVVARPRSLSEARE
                     DLQSAEHAVDVAIVDLNLPDGSGTDLIRELRSSLPQATALVLSATTDQKHLASAIEAG
                     AAGVMHKSAPMSDLVEAVRRLAAGEQLLSQQEVIGALRFLVRVRESNREAQLAIDRLT
                     PREREVLQALAEGWSDKEIATRLHVDVGTVHSHVTNILSKLEVSSRLQALVFAVRHGV
                     VTIE"
     gene            10502..10813
                     /locus_tag="VFQ10_08330"
     CDS             10502..10813
                     /locus_tag="VFQ10_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965388.1"
                     /translation="MRALIANEPAIYREVISAALKELRPDIEIFTSEPNDLEGEFLRI
                     LPQLVVCSRPTKLVEREAPACVELYPGGASHAVVKSLDGSRRTLPTMDFDTLLSILDG
                     I"
     gene            10989..11276
                     /locus_tag="VFQ10_08335"
     CDS             10989..11276
                     /locus_tag="VFQ10_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965389.1"
                     /translation="MTRRMAILVAVVGLMVMAFAGTALAVVKVGNAGPNRLVGTAEND
                     TLKGRSGADTIIGKGDSDRLYGGHGADHIKARERGRAEDDLVDCGRGETRS"
     gene            complement(11455..11892)
                     /locus_tag="VFQ10_08340"
     CDS             complement(11455..11892)
                     /locus_tag="VFQ10_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9965390.1"
                     /translation="MATVDDFDEVVEQYYLALNEFFKGNPEPVKSFFSHRDDVTLANP
                     LVGLPARGWERVAATLEHASSQFIDGENVGFETVAKYVTPELAYVLLIERDKVKVGGS
                     EDTAPSALRVTMIFRPEDGTWKVVHRHADPITTPQPAESVIQE"
     gene            12204..12713
                     /locus_tag="VFQ10_08345"
     CDS             12204..12713
                     /locus_tag="VFQ10_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965391.1"
                     /translation="MSEGNLDHEQELTRLLDELGQTLGRRERVLEGMKRMRTQARLHE
                     ITVEADQAFEGWNQDMLVEDLQRELDHQRAHELELQRVLDLLERQREIEDRLFEELDR
                     HLVTEHRDDLGELWRRMERRDAIFEDITAGSLEQKRELIPEAMNCQRGLEDGLFKLIE
                     RHLTRMRRS"
     gene            12846..13076
                     /locus_tag="VFQ10_08350"
     CDS             12846..13076
                     /locus_tag="VFQ10_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965392.1"
                     /translation="MKGLRLVEIRQSSEAPAIADVSLAVREQVRKLLRRRNLPPGSRV
                     AITAGSRGIRDAVESIEDVALGGCPSSEVSQW"
     gene            complement(13489..14343)
                     /locus_tag="VFQ10_08355"
     CDS             complement(13489..14343)
                     /locus_tag="VFQ10_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9965393.1"
                     /translation="MTVLACTYGQREVDGVEGKRFEQIIVTVEAGIAEVTLNRPDRYN
                     ALGSRMVGELGEAVEEIEGSGEVRAMILTGAGDKAFCSGVDLKERAEMDADGRWSHNR
                     TLGAFAGRLARLQVPTIAALNGLAFGGGLEIALACDFRLTAEGASFALPEVGIGIVPG
                     AGGTQRLPRLIGVMRAKELILTGRRISTEDALDMGLVSKVVPRSSLMEEARSLAQEIA
                     ANSPLALAYAKAAVDLASETTIEQGLRYETAAIRATLSSEDYKIGLAAFAEKRPPEFP
                     PLTSRRMT"
     gene            14416..15555
                     /locus_tag="VFQ10_08360"
     CDS             14416..15555
                     /locus_tag="VFQ10_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET9965394.1"
                     /translation="MVDIAVVGAGTMGAGIAESSALAGLSVVMVDVREEALDLGRHTI
                     EKDLDRRVRKGRLSEEERRASIGRVSATTSLESCADSPVVIEAVVEDMAVKTRVFGDL
                     EGVVASEAVLATNTSSLSVAGIAAATEHPGRVVGMHFFNPVPAMRLVEVVTGPSTDPS
                     AVSQVEEMAERLGKTPIRVSDTPGFVVNRVARPFYLEALRLVEAGGKPGQIDAAIRGA
                     GFRMGPLQLADLIGNDVNLTVSESLFERYYYHPRFRPSYLQRSMVEGGLLGRKSGRGF
                     YDYSAEKTAEDNEIEPSEDVSLRVISCIVNEALVALSEGVATAEDIDRAMKLGANYPK
                     GPFEWVEEIGARSIVETLDSLRAANGDAYLAAPALRERATGTRPP"
     gene            complement(15603..16007)
                     /locus_tag="VFQ10_08365"
     CDS             complement(15603..16007)
                     /locus_tag="VFQ10_08365"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9965395.1"
                     /translation="MKDKNLEASSEFVRVAGLRFDEISGKRVRGKIELDERHHTPWGV
                     VHGGVYTSAVASAASVGASTAVEGRGQFAVGVSSSTDFLRPVMEGGVEVVAEPIMQDK
                     IQQLWQVTITRSDDGKEVVRGRVRLQNVPLPS"
     gene            16383..17882
                     /locus_tag="VFQ10_08370"
     CDS             16383..17882
                     /locus_tag="VFQ10_08370"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9965396.1"
                     /translation="MAQAFIHGGYTDPSGVELMSIIDPSNGEEVDTVPASGTVEVDRA
                     VGSAYDAFPAWRDIPAAKRGEILGKAAEAVLAAREELAPLLTSEQGKPLREARLEIRR
                     FVHTLEHYVGLARNIRGGYVPDLDEDTYGLIIRRPLGVIGAIVPWNFPTTLLGNKLGP
                     ALITGNVVVAKPADTTPLTTLRIAEIMHEAGLPAGVFNVITGTGEGAGQALAEHPLVR
                     KIAFTGSTPTGKKLMAIAAGDLKRTTLELGGSDPMIVCDDANLDKAASAASVGRFFNC
                     GQACLAIKRLYVFDSVADEFIEKLAAKVQKLRVGPGTAEGVMLGPLHSAQQRDLLEEQ
                     VADAVDTGAEVLHGAKRPEGDDFEKGYFYEPTLLLEPARDSRVAKEEVFGPALPVWRV
                     SGMEEALELANGSIYGLGSSIWTADLNRASDAAERLEAGYTWINSPQLVYDELPFGGL
                     KQSGYGKEHGIEALDYYTETKSVVVKRAVATNKEAATRGLGGPESVSQR"
     gene            17889..19469
                     /locus_tag="VFQ10_08375"
     CDS             17889..19469
                     /locus_tag="VFQ10_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET9965397.1"
                     /translation="MIEIPEWYNASVLVDRNLEAGRVDKVAIYCGDDEVTYGELARRI
                     SRFGHALRALGVDREDRVLLMLNDTPSFPTAFFAVMRIGAVPIPVNTLLQPVDYRFFV
                     ENSRARVVVCDQMHHETIRKALEGYDEEPVEVIVTNGRAEGARILEDLLAAGEDELAP
                     VATHRDDPAFWLYSSGSTGKPKGAVHLHHDIIYTCETYARHVLEVSEDDICFSASKLF
                     HAYGLGNNISFPYWAGASTVLFPGKPMPDAILGTTQRFNPTLFFSVPTLYNAMLNREG
                     AGDYDLSSIRLCVSAAEALPAGIWRRWKETFGSVILDGIGSTEMLHIFVSNTPEVIKP
                     GSSGKPVPGYEAKILDEAGYPVEPGEAGYLSVKGDSAAAYYWRNHEKTKQTMQGEWLF
                     AGDWYRVDEDGFYWYEGRADDMIKVSGLWVSPVEVESTLGDHPAVIEAAAVGVPVDGL
                     TRVRAHIVLRQEYEPSEELVAELQNWCKERLKRYQYPHLVEFVEELPKTVTGKIQRFK
                     LREPETGVQMPRQEDELV"
     gene            19479..20747
                     /locus_tag="VFQ10_08380"
     CDS             19479..20747
                     /locus_tag="VFQ10_08380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET9965398.1"
                     /translation="MTRIKEAWIVGAVRTPIGRHGGALGAVRPDDLGAVALEALLERT
                     GLPPEEVEDVYFGCANQAGEDNRNVARMSLLLAGLPETVSGATVNRLCGSGLEAVASA
                     ARAVMLGEADVYIGGGVESMSRAPWAVPKPERAFPTGNTTMYDTTLGWRFVNPRMEQM
                     GHTDSLGMTAENLAQERFKVSAEEQKPEELAHTYDISREKQDRFALGSHKKAVAAMDE
                     KRFREEIVPVTVKGRKGDTVVEADEGPRRDTTLEKLAKLRPAFKEGGSVTAGNSSSLN
                     DGAAAVMVVSRDYAEAHGLEPMAKIRSVGVAGVPPRVMGIGPVPATRKALDRAGISLE
                     GVGLIELNEAFAAQSLAVLYEWGMDPDDKRLNPNGGAIALGHPLGASGARILTTLVHE
                     MARRPDVRYGLATMCIGVGQGIAMVVERGR"
     gene            20782..21663
                     /locus_tag="VFQ10_08385"
     CDS             20782..21663
                     /locus_tag="VFQ10_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9965399.1"
                     /translation="MLNGTPLIDAHVHAARLPTLKESWREWAAQHGSPSLLELYNEEG
                     TLVPQRFDDYMEREGVDVAVLFCEYSPKSTGIQPVEDLIPLLSHNPERFRLMANLNPH
                     LHFPVVDELERQIELGAVGLKVHPVHGSFAPNDRMLYPAYAYCEREGFPVVFHCGTSV
                     FPGSSNRYADPALIEDVARDFPDLTMVLAHGGRGWWYDAAAFITLMRPNVWIEVSGLP
                     PHKLPDYYRNFDFERLARKMLFGTDWPGVPGPGSNAQAIANLDLERETLERIFYKNAL
                     RVYERIGAGNLTIIDID"
     gene            21709..22656
                     /locus_tag="VFQ10_08390"
     CDS             21709..22656
                     /locus_tag="VFQ10_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HET9965400.1"
                     /translation="MKAVTEEILLDRIKSGRLIEDSSQATERYVEGLKRTLIVSADTE
                     LISAPAYMNATKDFSTLPSVNNYITVMGIVQDELGHAHIAYRMLRDLGVDTEELVYER
                     KPRQFKYPYAFDVPLESFTELIVANGFYDRAGFVLLSDIHHNCSYGPWKRALVKVDRE
                     ENFHLRHGEKWMKAFSRDPEKKEELQRAVEWMFPLTLEWFGLPDGQKRHNEQIEYGYK
                     GSTNDQLRQTWMSHTVPLCESLGLDVPAHYDEQEDTYFIDSPFPAQFDAEEKRWEFEE
                     GEISWDDVLVRWRERGPKNEEYVEHLQRGYKELYAREAV"
     gene            22659..22988
                     /locus_tag="VFQ10_08395"
     CDS             22659..22988
                     /locus_tag="VFQ10_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET9965401.1"
                     /translation="MQEKIIAAGVTAEEAREALREVFDPEYPISLVDLGLIRGVEVEG
                     SKAKIKLTYTCMGCPAMDMIQDDVRDRLLEMDGIDEVAIEVVWETWSRKDITPLGRKQ
                     LKDVGVV"
     gene            22988..23182
                     /locus_tag="VFQ10_08400"
     CDS             22988..23182
                     /locus_tag="VFQ10_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965402.1"
                     /translation="MRPVENYEVFTARVGEAPLRHNGNVRAAEPRDAAVYAHLMYDEW
                     RWREMFVVPSRAIVRVIRPE"
     gene            23189..23401
                     /locus_tag="VFQ10_08405"
     CDS             23189..23401
                     /locus_tag="VFQ10_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965403.1"
                     /translation="MVERYMVFARNEYDEPLEHRGDVEAAGNDDAAKLAKERYGQDWL
                     EMSLVPVSKAYWAERETEVGETEVQV"
     gene            23398..24231
                     /locus_tag="VFQ10_08410"
     CDS             23398..24231
                     /locus_tag="VFQ10_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HET9965404.1"
                     /translation="MSKRPEGTEQVAPGRGAQSEFLVENDDALAALVNLIAVIGDNVY
                     FLGRRVSEWADAGPLLESTAACAAITQDKLGQSRAIYPLLEDLPWPNPPTGLQGEVDR
                     ARRYSVSFLDDPLPSWTNVVAALALVGPAVNTVLEAVEHSRYEALARRAHRILDEERL
                     TSAYAESLVRQIAYEERGRNLFQVRVDELLPEMLCWFGPDGEEGIEALRAEGLIDMGN
                     EEMRQRYLGKVVPLLQECTIEVPIEWSESERRWEYGELPWREWNQLQRRLARSAQRSG
                     V"
     gene            24365..25132
                     /locus_tag="VFQ10_08415"
     CDS             24365..25132
                     /locus_tag="VFQ10_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9965405.1"
                     /translation="MRTTLDDSALLVELNRPEKRNALDETLQGELLQVFTDAAHDRKV
                     RGVILTAAGEAFSAGGDLSRFERDWEQAEFRAHSQELTHLISSIERLEKPVIAAIGGL
                     ATGAGTQLALSCDLRIASENARFVFREGMIGLIPSHGGCVRLVKLVGLARARDIVLGG
                     QDLDAEEAYRHGLVTEIVPHEKLLDEAKERLRFILKRAPQAYGLSKRLLHFSTSVDIE
                     SGLFAESMAQSLLVGTEDHTEGVRAARERRAPSFTGR"
     gene            25137..>25466
                     /locus_tag="VFQ10_08420"
     CDS             25137..>25466
                     /locus_tag="VFQ10_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET9965406.1"
                     /translation="MGYLLPFEDKRPLVADDAFIAPTAVLIGDVVVEEGASVWFGAVL
                     RGDFNRIVVGSGTSVQDNSVIHTNEELPTIIGSNVTVGHLSLLEGCEIEDGALIGMGS
                     IVLNRARV"
BASE COUNT         4796 a         7837 c         8149 g         4684 t
ORIGIN      
        1 gcaaaccggc gtccccggag gagatcgccg ccatctccgc caagtccccg gaggaggtgc
       61 gagccgcact cgaccggttt ccaagcgccg agtgggacga gcaaggcagg gtcgtcggcc
      121 tgggcttgac cctccagctg accccacatc gcttggaatt ggagggccgt atcctgtttg
      181 cctggtgcgc gctggacgcc ttactcttcc ccatactctt aggacggccg gcgagcatag
      241 agtccccctg ccggggcacc ggtgagctgg tgcacatcga ggtgacgtcc gtcggaattg
      301 aggcggtaga gcctccgtct gcagtggtgt ccatcgtcgc ggcgcgcgat ctggccacct
      361 tccgcagggt cagttgtaat aacgcgcatt ttttcagctc cccggaggca gcatcctgct
      421 ggctcgagaa gcacccggaa gcgaccatcc tctctgtgga ggacacgttc cggctggggc
      481 ggctgatcgc ggaagacctc tcagatattt cccgctcggc cgagccggcg gatgccggac
      541 gcgaatgaac cggctgagga agggaacggt gtcggagggt tctgaagtcc atactcacac
      601 ggtatcgaga ggaggttaaa aggtgcaaca gaagcacacg accggtgctg ggttgttgga
      661 ggagagcgaa agtagaggat aaatcatgat tgagcccaag aactttcgcc gcccgaaagg
      721 agccgccaag catcgatgga tcggggtcgt ggtcgcgctc gtgctgggcg cgttcgcggc
      781 gatttctctc ctcacgctcg aacccccaaa acccctgccc gcgagcgccc aggcgagtga
      841 gttctcggcg gagcgggcct tctcccacgt cgagcagatc gccgagcggc cacatccggt
      901 gggttctacg gccaacgccg aggttcgcga ctacttggtc gggcaactgg aggatctcgg
      961 gctgcgcccc accgtcctgg aggcaacgtc cgcgcggacg aaggagggga ccgcctctat
     1021 tgcgcgcgtg cacaacattc acgcgcggat ccctgggtcg tcgccgacag gccacgtcgt
     1081 gctcgtcgcc cactacgact cggtgccgag aggtcccgga gccgctgacg acggtgccgg
     1141 agtagccgcc atcctcgaga tagcgcgggc gatcacctcg agttccccgc cccggaacga
     1201 catcgacatc gtgtttaccg acgccgagga gccctggcta ctcggcgctc aggccttcgt
     1261 gagagaaggt cggctcgacc cgcaacgcag cgtcgtgctc aacctcgaag ccggcggcac
     1321 ctctggtccc gccttgatgt tcgaatcgag cctggacaac gcaaccctca tcccggcgct
     1381 cgcgagcgcg cagcgcccga tcgccggctc ggggtgggag gcatgctacc agctgctgcc
     1441 caacgacacc gacttcaccg tctttcgcga tgccggtttc gccgggatga acttcagtct
     1501 cgtggaaggc tcggcccgct accacacacc ggaggactca cccgccaacc tcgacccggc
     1561 gagccttcag cacatgggcg caaccgttct cgctaccgcc caccacttcg ccgggcaagg
     1621 tctgggggtt ccgcgcggtg ggtcgctcac gtacttcacc gtgctgggag agctcgtcca
     1681 ctacccgcag cagctggccg tcccgttggc ggtcctcgca gcggtggtgt tcgtggcgac
     1741 cctgctgtac gcgcggaagc gtggagcgcg cggcgtcggg tgggctgcgg tgagtttcct
     1801 cgggcttctg gtggtgacca cggcgctcgg gataggggtc tggcaggtgc tgcgtctcct
     1861 ccaccccggc tacgggtcgt tccataccgg cgacacctac cggcccgagt ggtacgccgc
     1921 aggcctgctc gcgctgacag ccgcgctcac cgtcgcctgg cacctgctga tgcgccggcg
     1981 ccacgcacca gaggagctcg cgcttgcgac ctgggcctgg ttcgtcgtgc tcgccatact
     2041 cacggctgcg ttcgtgcctg gggctgccta cctgttcagc tggaccccgc tcgtgggcag
     2101 cctgctcctg gccgccgcgt tccgctggag cggggaggag ccgacgtggc gctatctcgc
     2161 cttgagcgcg ggcgccatac cagccgtcgc gttgctgggg ccgttggtcg acctgttctt
     2221 cttcctcggg ctggctttcg ccagcggtcc gatgttcatc gcggtcctgg ctctggccac
     2281 cgctctgccg gtgctggacc ttctcgcgct ccgccacgcc tagctcgtcc ctgtggcggc
     2341 gacggtggta gagctcgccc tgttcgccac cgggctgagc atcgacacct tcgacgatga
     2401 gcacccccgc cagaccagac tcgtctacgc cctcgacgcc gaccaagagc aggcgtggcg
     2461 gtatctcgcc ttgagcgcgg ccgccgtacc ggccgtcgtg ttattggtgc cgttggtcga
     2521 cctgatattc tccctcgggc tggctttcgc cagcggtccg ctgttcatca cggtcctggt
     2581 tctggtcacc gccctgccgg tgctggacct tctcgcgctg cgccacgctt ggctcgtgcc
     2641 ggtggccgca gcgatggtgg gtctcgtcct catcaccgcc ggactgcgca tcgacacctt
     2701 cgacgatgag cacccccgcc agaccagact cgtctacgcc ctcgacgccg accttgagcg
     2761 ggcgtggtgg ctaagcgcgg acccccaacc ggcaccctgg accgaccggt acgtcgacac
     2821 agagcgcatc gaagacgacg accggtttcc cgacagcccg agcctctccc tctcgtcgcg
     2881 ctactattca ggacctgcgt ctgtagcccg gatcgagcca ccggacgtca cggtgagacg
     2941 aagcgaccga gacggcgaca cgcgcgtcct tcggctgcgc gtcacccccg aagagggctc
     3001 gcgcattgcc ctctacgccg acacgaggag ccacaccgtg accgccgcca ccgtcgacgg
     3061 catcagcgta gaggaggcgc ccggccagag gcaggacacc gatccatcta agtggggctt
     3121 cgtattccac acggtaccgc ccgaagggat cgacgtgaca ctcgaggtgc gcggggaagg
     3181 gctgctgccg ctgcgcgtta tcggctaccg cgatggcctc ccgcaagtcc ccgagctgac
     3241 gccccgcccg gacgacctta cctggtcagg aacttcgtcc aacctcacca tggtcgccga
     3301 gagctaccgt gtgtagccca cccggcgaag gcactcgtgc gtccagcgtg ccggcaacgg
     3361 agcgcgccaa gcagttgaag tttaatagcg atctggcagg gtggggaagg cctcactctg
     3421 aggtgtccac tggcatagcg gtagaagtcc ctctcgctgg agaatctgga ccactgggcg
     3481 aagatggcga gggtgatgac ttcgctgggg ctgaggcaag catcaggacc tgggcatcta
     3541 ttcgaggcat gggagtggca gcaatcgtcc accatgacgt aaagtgcggt gaggaaggtg
     3601 tccttaggtt gagcatgcgg agtcggttcc tttcccttcg ggaaacggca taggcaagcc
     3661 gactctaatc cgtgggcacc ttccactcaa ctctcgctct aataactcac accaagcgtt
     3721 taagggcgta cccttcaagg tgccgctgcg tttttcttca aggccgggtc gaaaaccttc
     3781 caagcaatcc cggatctcgg agatcttcgc ccgtttgtca cgtttatgct ctgagtcgct
     3841 gtctcttccg gacccctcga ggttcgaccg ctatcggagg cggtacagac gggtacttgc
     3901 cgcagtcaat ccttatccga gttgtttgta ttcaagagtc cgaattctta ttcacgagta
     3961 caatatgtcg tgaggagcta ggaggccgct gacaagacga aggatctcct ggcaccttgg
     4021 cagctagagt ccatgcaata gcgtggatat agacttctga gaattcacct gttacgagat
     4081 tcggtgaatg gacctttcgc gttcgtctca cacggagatg acgacctttc ctcgggcgtg
     4141 tccctcttcc agataacgga tggcctcggg tacctcggcg agcgggtatg tcctgtcgat
     4201 gatgggtgtg accttaccgg cctcgatgag ctccttgagg acgagcatgt cctcgtggtt
     4261 ctccttgttg acgaacgtgc ccagcttctg acccacgaac ggggacagca taatcgcccg
     4321 gagctggcga tcagtgcccc cgagccaccg tcctccgcct tcgcctccaa cgatgacgag
     4381 ggtcccccgg gaggcgaggg cccgccgcag gcgtgacaac gtcgagttcc cgccgatgtc
     4441 gaggatcaga tcgtagcgct gatccccctc ggcgaagtcc tcgcgcgtgt agtcgatgac
     4501 gtgatccgca ccgatggatc tgaccatctc caccttcttg gtgctgcaca cgccggttac
     4561 gttcgcgccg aacgccttgg cgatctgcac ggcgtaggtc cccactcctc ccgacgcacc
     4621 gatgagcagc acttcctgcc ccgggctcac ctttccgtgg tcgcgcaagg cttgcagggc
     4681 ggtcgacccc atgatggcca ccaccgccgc ctgctcgaag gtgaggtttg tgggcttcgg
     4741 tgcgagcttg tcctcgcgag cacacacgta ctcggcgtag gagccctttc cgatgccgaa
     4801 cacctcatca cctggctgga agcgggtcac gttcttgccg accgcctcca caaccccggc
     4861 catgtccgaa ccgatgacgg ggttcttggg cgcgcggaag ccatagcccg cgaggcgtat
     4921 cgggtacggt agtcccttca tgatgtgcca gacgtcccga cccacgccgg cagcatgcac
     4981 gtgaaccagt acctcctcat ctgcgtactc cgggatgtcg atgtccctga gttccaggac
     5041 ctcgggtgag ccgtaggtgt cccgtactat cgccttcaac gagttctcct cctttcccca
     5101 ctggagtggt tgcacctaat gtaacagcat cgcgaggagt ggtacatact ccaaaatgga
     5161 tggtctttga acctttcctg gcgaatttcc gagaaacacc taaaacacga actacttaga
     5221 actccgtcaa gccgaaggtc gcagagcgct cctttcacgc actcaggtgg ataccgttgt
     5281 cttgctattg cctgagctcc tcgatcctcc acaagtctcc ggtgccagcg gcgcagtcgg
     5341 tcgattcatg ctatctgcgt cgggcgagca gtccccactc gacagcttct tgcgccacga
     5401 gatcggcacg ggcgctgttc agcccaacgc ctgcctcctt accgatcgtg aggttcggaa
     5461 gtaggtgttg gcggccggta ggaacatcaa cgccagcgcc gcgattaccg ccgccagatg
     5521 aacactccgg atcggcgcga taaggaagaa caggagatta gcctccgtga agacctcact
     5581 cagcgagtta cccccagcca gccacgagat ggggtcgata accagggaga gagtcccgat
     5641 cacgccgagc agcaccgcta gggtcaggcg ggcccatctc ttcccgagac gcatccgtgt
     5701 gattatgtaa accaccagcg cgtaaacgat caccctgata accacgccag agatcgcacc
     5761 ggtgacatcc gtctccccgc cgctgcccga ggcgaagcct atggccgagg cgtcgataac
     5821 acggatgatt gtttcgagga cgcctgcggc gacggcggta agccatagta agaacgatgc
     5881 ttgcacctct ttcggcacgc ccggcggtcg gctcagcttg aaggttgcca tccccgtagc
     5941 cacgtctttc gaggggctgc gcgggttcat cgaagcatgt ccatcatgag tcgctcttcc
     6001 tttgtaggtc gactgtcgag agcgtacctg cgggcaccgg ctgtatcatc ggtccagggg
     6061 aggaacatgg atgggtcgtg aggacgatcc gggccggact tagaagacac tctttctagc
     6121 cattagtgcg agcggaggat tgagggtgaa gtccgcgcca ttcctctact aggcagatgc
     6181 ctccggccct cgcgttatgc tgggtgaggt gcgcctctgc gtgtatcctt ctgccggaac
     6241 aatatccatg cacctgccca gcgtcgtggt cggctctagg tgcagtcgcg tactctaccg
     6301 acacacgggg tccttcgacg cgttgccgtc ttttttgttg ctgtacaggt acatggtgag
     6361 cggcgcgaca acggcaacga gtacccctga cgtcaggagc atccagccga tctgtccggc
     6421 cgtcgcggtg cggtgcatca ggccgcgtac cgccgtcgcc gggtgggtga tcgggttgga
     6481 accgacaatc gcctccagcc acggggatgt tgctcgcgaa ggtggtacac gacgatccga
     6541 aacgcccctc caaccacccg cttcgcaacc ttgagtcggg tctcccacaa gccgggcgag
     6601 cgcgctgcgg tcttgcttag cgctctaccg ctacgatctc gattctttcc agcttcgact
     6661 cgcggttcgg cacgtttcac actatcattt ctcaaacgag ctccctaatt tctcacccgc
     6721 cgacgcactt gggtaccggt agaggtgcca ccgcgccgac cgagacgtgg gaccaagagc
     6781 gggcgctcca ccgaccgacg ccccgaacgc caaaactggg taacaacgct tgaacccacg
     6841 catgccagtg ttactcatgc gttcagtcaa actcttctcg atcatcgaag taggcctctg
     6901 ctgcgtcgtc ctcctgcgga ggcgctgcct gctgagcgta cacggaggcc gtgaacagcg
     6961 tcaaggttac cgcggccacc aaaccgacca tcaacttctt cgcgttcatc gttctccttt
     7021 ctgagccctc cctggtgagc acttcccggt catggcggtg ctaataatgg gcgcccgggg
     7081 cggactgtac atcggcagaa ttgggagtct ttctccaaag agagggggag gccttagggc
     7141 gtcccccctc atatgtccga ggcgttctag cgacggcgca cgataccgag agccatcagc
     7201 ccccgccaag gatcatcgcc aacggcccga gtacccacgc cggagggaag ccgctggtgt
     7261 ccgggagcac gggtgtggca gatgccgtag tggtcggaga ggccgtagga ctcgcgctgg
     7321 gagaagccga cgcactcgcg ctaggcgaag cggtagggct cgcggaagcc gaggcgctgg
     7381 aggcggggct gcccgccgcg ctgtcgccta ctccgcggtc gtccatcccc ctgcccctgt
     7441 catcgaagcc tcgatcatcg ctcgcggaag ccgaggcgct gctcatcgcg ctcgttgagg
     7501 ccgaggcgct ggaggcggga ctggccaccg cgttgtcgtc tactccgcgg ttgttcatcc
     7561 ctctgtcatc gaagccttta tcatcggccc ctcggacatg catcccccta tcgtcgaagc
     7621 ccttatcatc ggtccctctg acatccatac ccttatcgtc gaagcccccg tcgtctccct
     7681 gggcggctgc gacgggtgcg aacaccgaca cggccaacgc caccgacaag acaagtaata
     7741 accatctacg catcgtttct cctctttttc gcttgcacat ccagggaaag gatgcaccac
     7801 ccaggttcgt atgacctata caaatttatg gatcttttct cattgctatg gcagcgggtc
     7861 agatggcggt gaccctttga gtggttccct cgcatggatg cccgggcaac cctcgaccga
     7921 ttggcgaacc gatctccagc gtgccaccct gcgtatgtac tgaagggaga ctacactctg
     7981 gtctcccttc ctgaagggtg cagaacattg cagaggaggt tcgttgggag cgaagggcca
     8041 ctctcgggag agtatgatag gggagccgcc aagcctatcg ggttccctga gcggcaacag
     8101 tagcgtgctt gtcgccgcgg tgctggcggc cctcgtcgtg gcgtgggcag caacgaacct
     8161 ggtgttgggg ggtgaggggt tcgcggtgct cgtctcggac aaggtggtgg gcggggagga
     8221 ggcggcctcg gctctggctc gcctcttcgc cgccctcgtc ctcggactgt tcctggctga
     8281 cgctgtaggt tggcgtgcac gctgggtagc ggggggattg gtcgtcctgg gcctgggaca
     8341 cttcgtattc gggtacctgg agccgctgat tcaggaggat cctccggaac tcaacgagtc
     8401 gctctacgaa gccttcgtca cccaaacgct ggagtgagca ctgttcatgg taggtctgtt
     8461 ccccggaagg ccgcctcggc tcgtggtgtg ggctgcaacg gcaatcccga tcgccctggt
     8521 agccggctac gtggtcctct tcgagttcct gcatgcagaa gagtggatgc ccctcctatc
     8581 ccgcgtcgcg aacccggaga gaacagtgac gctaggctcg cccctagtct gcctgacgcc
     8641 gtggcactgg gcgctctctg ccctgccgct agggcttgcg tcggctgccg tagtcggagc
     8701 cttctggcag agtcgccgtg ggcttctgcg gagctggttg ctcattgcgg tggtgcttct
     8761 cgccgggtcg gtcttgcacg attacctgtg gccctcggcc tacggaggcg gcctgcttac
     8821 cactgccgac gccctcagcc tggtcttcgc ggtggtggtc gcgatcggcg gcgtttccga
     8881 gctccgacgc gtcgcctccg agcgcgccag gcttctggct accgagcggg agcgcgcgcg
     8941 gcgactaaac gaactcaact cgctgaggtc cgacttcagc gcgatgattg cccacgagtt
     9001 agatacgccg atagccgccg tccgcaagct caacgagatg ctctctgccc agggggagga
     9061 tcccggggta cgcgactatg ccacgaccgc aacggagcgg gaactcgacg ctctgaccaa
     9121 cctcgtcagg gacgtgcgag ccgttgctgc ggtcgaacgc gagggtttcg ggataaaggc
     9181 gcgccctctg ccgttggagg aactcctggc cgacgccgag gtgtacgcga gtaccctacc
     9241 tgggcaccat cccatagagg tgatggttcg aggggatctc agggtggagg tttgtgtgct
     9301 cgccgacccc gagcgtatcg ggcaggtctt gcgcaaccta ctctcgaacg ctgccaaata
     9361 ctcgccagaa ggcacgccca tcgagctgcg ggtgatcggc aaggaggggc aggtcagggt
     9421 agagatcgcc gatcagggat ggggtatcca cccggatgac gtgccacgga tcttcgagaa
     9481 gttcggccgg ggccgcgacc ggaagggtca tcacgaaagg ccgggtgtgg gccttggcct
     9541 ctacctcagc cagcgcatcg tcaggggcca cggttccgag ctgacggtgc agaccaggcc
     9601 aggcgagggc tcggtgttcg ggttcgagct aagggtggaa cggtgaacag ggagatcagg
     9661 atcatggtcg tcgacgacca cgataccttc cgcgacccgc tcgcgttcat gctggaacga
     9721 gagccggatc tcaccgtggt ggctcggccg agatccctgt cggaagctag agaggacctc
     9781 cagagtgccg agcatgcggt ggacgtagca atcgttgacc ttaaccttcc cgacggttcg
     9841 ggtactgatc tcataagaga gctgcggagt tccctaccgc aggcaacggc gctggtcttg
     9901 agtgccacta ccgaccaaaa acacttggcc agcgcgatag aggccggggc agcaggggta
     9961 atgcacaagt cggcgcccat gagcgacctc gtggaagcgg tgcgccgcct ggccgccgga
    10021 gagcagctac tctcccagca ggaggtcatc ggggccctgc ggttcctcgt gcgcgtgcgc
    10081 gagagcaatc gcgaggcgca gctcgccatc gacaggctga ccccgaggga gcgcgaagtg
    10141 ctccaggccc tcgccgaggg ctggagcgac aaggagatcg ccacgcgact acacgtggac
    10201 gtgggaacgg ttcactccca cgtgactaac atcctctcca agctcgaagt ttcctcgaga
    10261 ctgcaagcgc tcgtcttcgc cgtgcgccac ggcgtggtaa cgatcgagtg acagtcttgg
    10321 tcggcccctg aacgtccggg cctccttgcc gatgatccgt gcggatctat acgaaattcc
    10381 agaggcgaag catcccaacg atccacacta tggaggacga tggagcgagc ctacacaacc
    10441 atgtgccgta aggtctcgac gtgcccctag cggccagggc caagataccg gcgcgagagg
    10501 catgagggcc cttatagcca acgagccggc catataccgg gaggtgatct ccgcagccct
    10561 caaggagctc aggcccgata tcgagatctt cacatccgag cctaatgacc tggaggggga
    10621 gttcttgcgc atcctgcccc agctcgtggt ctgcagccgg ccaacgaaac tcgtggagcg
    10681 cgaggctccc gcgtgcgtcg agctctaccc cggtggcgct tcgcacgcgg tcgtaaagag
    10741 cctcgatggg tctcgtagga ccctcccaac catggacttc gatacgctac tctccatctt
    10801 ggacggcatc tagggccctg aactcgggct cgtggtggat atccctagcg cgtgaattct
    10861 agaagaagcc cctgcgtcta tacgaatttg catagacgaa tatcgataat tctgaggatg
    10921 gctctcgtgc tccgaggatc catcatgtgc tttgtcggta aagacaacgc acctaaggag
    10981 gtaagcggat gacgagaagg atggccatct tggtcgcggt tgtagggctt atggtgatgg
    11041 cgttcgccgg gacggccctg gcggtcgtga aggtgggcaa cgccggtccc aacaggctgg
    11101 tgggtaccgc cgagaacgac actctcaagg gccgaagcgg ggcggacacc atcatcggca
    11161 agggagactc ggaccgtctc tacggtgggc acggtgcaga tcacatcaaa gcccgcgagc
    11221 gtggacgcgc cgaagatgat ctggtcgact gcgggcgggg cgagacacgg tcctgaccga
    11281 caacacaaca gaggatagga tcatgtcgaa ctgcgaggtc gttaggcggg gttagaaccc
    11341 ccaataacag aggcactcag gaccggggct actagcagct ctggcccttc ttctgcccct
    11401 attcgcccaa cctcgtagaa ggcgaagtct ccgaaggtcg ctctaagggt aggattactc
    11461 ctggatcacc gactcggccg gctgtggggt ggttatgggg tctgcatggc gatgcactac
    11521 cttccaggtg ccgtcctcgg gtcgaaagat catcgtaact cgcagagcgc ttggagccgt
    11581 atcctcgctt ccgccaacct tgaccttatc ccgctctatc aatagaacgt aggcaagctc
    11641 aggagtcaca tacttcgcta cggtttcgaa gccgacgttc tcgccatcta tgaactgcga
    11701 cgaggcatgc tccagagtcg cagcgacccg ctcccatcct cgcgcgggga ggccaacaag
    11761 agggttggcg agggttacgt cgtctcgatg ggagaagaag ctcttcacag gctcggggtt
    11821 gcctttaaag aactcattga gcgctaggta atattgctca actacttcat caaaatcatc
    11881 tacggtagcc atctctagat tcctccttta cccacatacc ctgcgatcat cttacgtcat
    11941 tcacccgact acctagaagg tgaattcttg gaagttgggc actccctgag gagctaagta
    12001 gaagggcttt cctcaggctg ctgcggaccg ctgtcctcac tccacgcctc cacaatcttg
    12061 ccctcctaaa cggcgtagac ggccctacta tatcccaaca tcttgtgcag gaccttcata
    12121 gaattctatc gggataattc tttaggatgc gacggcgcgc agggtttatc ctaaaggtta
    12181 gggctcgcga accggaggag cgtatgtccg aggggaacct ggatcacgag caggagttga
    12241 cccgactgct ggacgagctg ggacagacgc tcggccggcg ggagcgagtg ctcgaaggga
    12301 tgaagcgcat gcgcacccag gcgcggctgc acgagatcac cgtggaggcg gatcaggcct
    12361 tcgaggggtg gaaccaggac atgctcgttg aagacttgca gcgggagctg gatcaccagc
    12421 gcgcgcacga gctcgaactg caacgagtcc tggacctcct ggagcgccag cgggagatcg
    12481 aagaccggct cttcgaggaa ctggatcgcc acctcgtcac ggagcaccgg gacgacctcg
    12541 gagagctctg gcgccgcatg gagcggcgag acgccatctt cgaggacatc accgccgggt
    12601 ctctcgaaca gaagagagag ttgatcccgg aggcgatgaa ttgccaacgc ggcctggaag
    12661 acggcctctt caagctaata gagcgccacc ttacccggat gcgtcgatcc tgagaatgac
    12721 ctacgctcgt ctcctttacg cccgacgacg aaaccgggcc accgcgatcc agaggaccaa
    12781 cagcgcgagc acgtgatgaa ggccgatgtt ggctgcgact tcgactttct gaggaaggta
    12841 tgacagtgaa gggtctgagg ctcgtcgaga tccgccagag ctccgaggct ccggccattg
    12901 ctgacgtgtc gctggcggtg cgcgaacagg tgaggaagtt gctccggcgg cggaaccttc
    12961 cgcccggctc gcgagtcgcc atcaccgccg ggagccgcgg cataagggat gcggtcgagt
    13021 ccatagagga cgtggcgttg ggcggctgcc cgtcctcgga ggtctcgcag tggtgaataa
    13081 tgctcgcgag cggacggctt ctgacgaggg ctttggctac gaggggcacc cttttccgga
    13141 gtactgtcaa atctctgtca aaagagccag gcgatgaacg cagctctcca actgcgtctg
    13201 catttatcct gcaaaacgcg aggaatatat ggcgcgctcg gcaggactcg aacctgcgac
    13261 cttctgattc gtaggccgaa tcgggcggtt ctatgcggtt ctctgaagtg cgttaagggt
    13321 gattcttggg cactttgagc gtctcgaggc atcggtttgt tctgtcctgt tcccgtcata
    13381 ctcccgtcaa ctcggccggt gcggttcgcc acctcggggc tcacgatctg ggcgacacgt
    13441 gcgccagcga cccacccgga gtctccgccg cagaccgttt cgtgtgtctc atgtcatgcg
    13501 gcgagaggtt agcggcggaa actctggcgg ccgcttctct gcgaaagcgg cgaggccaat
    13561 cttgtagtcc tcggaagaaa gggtggcacg tatggctgca gtctcgtatc gaagtccctg
    13621 ctcgatcgtc gtttccgagg cgagatccac agccgctttg gcgtacgcta gtgcgagcgg
    13681 gctgttggcg gcgatttctt gggccagtga ccgcgcttct tccatgaggg aacttcgcgg
    13741 cacgaccttg ctcacgagac ccatgtcgag cgcgtcctca gtgctaatcc tcctaccagt
    13801 caggatcagc tccttcgccc tcatcacccc gatcaggcgc ggcagccgtt gcgttccacc
    13861 cgcgccgggc actatgccga tgcccacttc ggggagcgcg aagctcgcgc cctcggcagt
    13921 gaggcggaag tcgcacgcca gggcgatctc cagcccgccg ccgaaggcca ggccgttgag
    13981 ggcggcgatc gtgggcacct gcaaacgggc gaggcgcccg gcgaaggcgc caagggtcct
    14041 gttgtgggac catctgccgt cggcgtccat ctcggcacgc tccttcagat ctacccccga
    14101 gcagaaggcc ttgtctcccg cgccggtgag tatcatggcc ctgacctccc cggaaccctc
    14161 gatctcttcg accgcctcgc ccaactcgcc gaccatgcgg ctgccaaggg cgttgtagcg
    14221 gtcgggacgg ttgagtgtca cctccgctat acccgcctcc acggttacaa tgatctgctc
    14281 gaagcgtttg ccttccacgc cgtccacctc tctctggccg taagtgcacg caaggacggt
    14341 cattgtctac aatcttgacc gtccatacaa tcagttatgc cgagcagaag gcagctgcgt
    14401 cgtcaggaga tccggatggt cgatattgcc gtggtgggag ccggaaccat gggagcggga
    14461 atagcagaga gctcggccct tgcgggcctc tcggttgtga tggtcgatgt acgcgaggaa
    14521 gctttggatc ttggccgtca taccatcgag aaggacctgg accgccgggt gaggaagggt
    14581 cggctctcgg aggaggagcg tcgggcttcg atcggacgtg tctcggcgac cacttccctg
    14641 gagtcctgcg ccgactcgcc ggtggtcatc gaggccgtgg tcgaggatat ggcggtcaag
    14701 accagggtgt tcggcgacct tgagggcgtt gtcgcgagtg aggcagtgct cgccaccaac
    14761 acttcttcgc tctccgtggc aggcatcgcg gcggccacgg agcatccagg gcgcgtggtc
    14821 ggcatgcact tcttcaaccc ggtgccggca atgcgcctcg tcgaggtcgt caccgggccg
    14881 tccaccgacc cctcggccgt gagtcaggtc gaggaaatgg ccgagcggct gggcaagacc
    14941 ccgatccggg tctcagacac gcctggattc gtcgtgaacc gcgtcgccag gcctttctac
    15001 cttgaggccc tgaggctcgt ggaggccggt ggtaaaccgg gacagatcga tgcggcaata
    15061 cgaggggcgg gttttcggat gggacctttg caactcgccg acctcatcgg taacgacgtc
    15121 aacctcaccg tctccgaatc gctcttcgaa cggtactatt accatccgag gttccgcccc
    15181 tcgtacctcc aacgctcaat ggtcgaaggc gggttgctcg gcaggaagag cggtcgtggt
    15241 ttctacgact acagtgctga gaagacagcg gaggacaacg aaattgagcc ctcagaagac
    15301 gtgtccctgc gcgtcatatc ctgcatcgtg aacgaagcct tggtggccct ctcagaaggc
    15361 gttgccaccg ccgaagacat agaccgggcg atgaagcttg gagcgaacta ccccaaaggt
    15421 ccatttgagt gggtagagga gattggggcc cgcagcatcg tcgaaacgct cgactccttg
    15481 agggccgcaa acggcgacgc ttacctcgcc gcgcccgccc ttagagagcg cgccaccgga
    15541 acccgacctc cgtagtccac agctcatctg atcgttcgcg gcgcgcttgg tctgaggatg
    15601 cctcaggaag gcagcggaac attctggagc ctcacccggc cgcggacgac ctctttgcca
    15661 tcatcgctgc gggtgatcgt cacctgccag agctgctgga tcttgtcctg catgatgggc
    15721 tcggcgacca cttctacgcc tccttccatg acgggcctca ggaaatcggt gctgctgctc
    15781 acccccacgg cgaactgtcc ccttccttcg accgcggttg aagccccgac gcttgccgcg
    15841 cttgcgacgg ccgaggtgta gacgccaccg tgcacaacac cccacggcgt gtggtggcgc
    15901 tcgtcgagtt cgatcttgcc ccgaaccctc ttgccactga tctcgtcgaa tctcaggccg
    15961 gccacccgca cgaactcact cgacgcctcg aggttcttgt ctttcactgt gatgcccctt
    16021 tcggccaaaa tcctgggctg acgtcatgga accgtccacc taccagtgcc aaggctaacg
    16081 ccgcgccagc aaactagcaa tagagatgca gcgacatcgt ggcgtttcgc gcgttcgggc
    16141 cgacccgtgg accatatctc gatagaatct tgactgaccg ttcggttgac aggagtctta
    16201 ctggttggtt agataagggt ggaccaaagg gagttgtggt ttcagtcacg ggtaccgggg
    16261 aatcaacgtg gaggcggggt ggaaagggct gagatggtgt ccgccgtctt cgcaagctag
    16321 tgtgagattt tcgctgaccg gtatcctggc gtaggccacc aggtacgtaa gaggaggcct
    16381 tcatggctca ggcgttcatc cacggcggct acaccgaccc ttcgggggtc gagctgatgt
    16441 ccatcatcga tccgtcgaac ggagaggagg tggatacggt accagcctct ggtacggtcg
    16501 aggtggatcg ggctgtcggt agtgcgtatg atgcgttccc ggcctggcga gacatcccgg
    16561 cggcgaagcg tggcgagatt ctcggtaagg cggcggaggc cgtcctcgcc gcaagggagg
    16621 agttggcacc gctgctcacc tccgagcagg ggaagcctct acgcgaggcg cgactggaga
    16681 tccggcgctt cgtgcacacg ctagagcact atgtggggct cgccaggaac atcaggggcg
    16741 ggtacgtgcc cgacctcgac gaggacacct acggcctcat aatcaggcgt ccgttgggag
    16801 taataggggc catagtgccc tggaacttcc ctacgacgct gctgggcaac aagctcggtc
    16861 cggctttgat cacgggcaac gtcgtggttg cgaagcccgc cgacaccacc ccgctcacga
    16921 cgttgcggat agcggagatc atgcacgaag ccggcctgcc tgcgggtgtg ttcaacgtca
    16981 tcacaggcac gggtgagggg gctggtcagg cgctcgcgga gcatccgctg gtacgcaaga
    17041 tagccttcac gggctctaca ccaacgggta agaagctcat ggccatagcg gcgggcgacc
    17101 tcaagcgcac cacgctcgag ctcgggggct cggatcccat gatcgtttgc gacgacgcaa
    17161 atcttgataa ggccgcgagt gctgcgagcg tggggaggtt cttcaactgt ggccaggcgt
    17221 gcctggctat aaagcgcctc tacgtcttcg acagcgtggc cgacgagttc atcgagaagc
    17281 tcgcggccaa ggtccagaag ctcagggttg gtccagggac agcagaaggc gtgatgctcg
    17341 ggccgctgca ttccgcgcag cagcgagatc tcctggagga acaggtcgcg gacgccgtag
    17401 acaccggagc cgaggtgctg cacggggcca aaagacccga gggggacgac ttcgagaagg
    17461 gctacttcta cgagccgacg ctcctgctcg agcccgcgcg cgattcgcga gttgccaagg
    17521 aggaggtctt cggacccgcg cttcccgtct ggcgcgtctc cggcatggag gaggccctgg
    17581 agctcgcgaa cggttcgatc tacgggctgg gatcatccat ttggacggcg gatctcaacc
    17641 gggcctcgga tgccgcggag cggctggagg caggctacac ctggataaac tccccccagc
    17701 tggtctacga cgagctgccc ttcggcggtc tgaagcagtc gggctatggc aaggagcacg
    17761 gtatcgaggc cctcgactac tacacagaga cgaagtctgt cgtggtcaag agggctgtgg
    17821 caacgaacaa agaggccgcc acgagaggtc tcggcggtcc cgagtccgta tcccagaggt
    17881 gattcgtaat gatcgagatc cccgagtggt acaacgcgag cgttctggtc gaccggaacc
    17941 tggaggccgg tcgcgtggat aaggtcgcca tttactgcgg cgacgacgag gtcacctacg
    18001 gggaactcgc ccgccgcatc agcaggttcg gtcacgccct gcgggcgctg ggcgtagaca
    18061 gggaggacag ggtactgttg atgctgaacg acaccccatc cttcccaacg gcgttcttcg
    18121 ccgtgatgcg cattggggct gtcccgatcc cggtcaacac cctgctccag cccgtggact
    18181 accgcttctt cgtggagaac agccgcgcac gcgtcgttgt ctgcgaccag atgcaccatg
    18241 agactatccg gaaagcgctc gaaggctacg acgaggagcc ggtggaggtc atcgtcacga
    18301 acggtcgggc cgaaggggcg cgcattctgg aggacctgct ggcagcgggg gaggatgagc
    18361 tcgctcccgt agcgacgcac agggacgatc cggcgttctg gctctacagc tcgggctcga
    18421 ccggcaaacc gaagggcgcg gtccatcttc accacgacat catctacacc tgcgagacct
    18481 acgccaggca cgtcctggag gtctctgaag acgatatttg cttctcggcc tcgaagctct
    18541 ttcatgccta tggcttaggg aacaacatct ccttcccgta ctgggccgga gcttcgacgg
    18601 tgctcttccc cggcaagccc atgccagacg ctatccttgg caccacccag aggttcaatc
    18661 cgaccctgtt cttctcggtg ccgacgctct acaacgcgat gctcaaccgc gagggagccg
    18721 gcgactacga cctatcctcg atccgcctgt gcgtctcggc ggcggaggcg ttgccagcag
    18781 gcatatggcg gcgctggaaa gagacgttcg gctcggtcat tctcgacggc atcggctcga
    18841 cggagatgct gcacatcttc gtctccaaca cgcccgaagt gatcaaaccc ggctccagcg
    18901 gcaagccggt gccgggttat gaggccaaga tactggacga ggctggatat cccgtggagc
    18961 caggcgaggc gggatatctc tcggtcaagg gtgattccgc cgcagcgtac tactggcgca
    19021 accacgagaa gaccaagcaa accatgcagg gcgagtggct cttcgccggt gactggtaca
    19081 gggtggacga ggacggcttc tactggtacg agggccgcgc ggacgacatg atcaaggtca
    19141 gtggcctctg ggtttccccc gtcgaggtcg agagcacgct cggggatcat cctgcggtaa
    19201 tagaggccgc cgccgtcggt gtcccggtag acgggttgac gcgggtgagg gctcatatcg
    19261 tccttcgtca ggagtacgag ccatccgaag agctggtcgc cgaactgcag aactggtgca
    19321 aggaacgtct caagcgctac cagtacccgc acctggtcga gttcgtcgaa gagctcccta
    19381 agacggtgac gggcaagatc cagcgcttca agctgcgcga gcccgagaca ggagtccaga
    19441 tgcctcgtca agaggacgag ctggtctagg ggaggatcgt gacacggatc aaggaagcct
    19501 ggatcgtcgg cgcagtgagg acgcctattg gtagacacgg cggagccctg ggcgccgtgc
    19561 gtcccgacga cctgggggcg gtcgccttgg aagctctgct ggaacgtacc ggtcttcccc
    19621 ctgaggaggt cgaggacgtc tacttcggtt gcgccaatca ggcgggagag gacaaccgca
    19681 acgtcgcgcg catgtcgctc cttctggccg gcctgcccga gaccgtctcg ggcgccaccg
    19741 tcaacaggct ctgcggatcc gggctcgaag ccgttgccag cgcggcgagg gccgtgatgc
    19801 taggagaagc cgacgtctac atagggggcg gggtggagtc catgagccgc gccccctggg
    19861 ccgtgcccaa gccggagcgg gcgttcccta caggcaacac cacgatgtac gacaccaccc
    19921 tgggatggcg cttcgtcaac ccgaggatgg aacaaatggg ccacaccgac tccctcggga
    19981 tgaccgccga gaacctcgcc caggagcgct tcaaggtgag tgccgaggaa cagaagcccg
    20041 aggagttggc ccacacctac gatatctctc gcgagaagca ggaccgtttc gcgttaggga
    20101 gccacaagaa ggccgtggcc gccatggacg agaagagatt tcgggaggag atcgtgccgg
    20161 tcacggtcaa agggcgcaaa ggcgatacgg tggtcgaggc cgacgaggga ccgaggcgcg
    20221 acaccacgct ggagaagctc gccaagctca ggccagcctt caaggagggt ggatccgtca
    20281 cagctggtaa ctccagctcc ctgaacgacg gggccgcagc ggtgatggtt gtctccaggg
    20341 actacgcgga ggcgcacggg ctcgaaccga tggcgaagat aaggagcgtt ggggtggcag
    20401 gggtgccgcc gagagtcatg ggcatagggc ccgttcccgc cacccggaaa gctctcgaca
    20461 gggccgggat ctctctggag ggcgttggcc tgatcgagct gaacgaggcg tttgcggcac
    20521 agtctctggc cgtcctctac gagtggggga tggaccccga cgacaagagg ttgaacccta
    20581 acggcggagc gatagctctt gggcaccctc tgggggcttc gggcgctcgt atcctgacga
    20641 cgctggtgca cgagatggcg cggcgtcccg acgtgaggta cggccttgca acgatgtgca
    20701 taggggtggg gcagggcatc gcgatggtcg tcgagagggg ccggtagtcg tgtcggcgca
    20761 cgacgcggat agggtaccgg cttgctgaac gggaccccgc tgatagacgc ccatgtccac
    20821 gccgcgcgtc tcccgaccct gaaagagtca tggagggagt gggcggccca gcacggctcg
    20881 ccttcccttc tcgagctgta caacgaggaa ggcaccctcg tcccgcagcg tttcgacgac
    20941 tacatggaac gcgagggcgt ggacgtcgca gtcctcttct gcgagtacag tccgaagtcc
    21001 acaggtatac agcccgtgga agacctgatc ccactcctct cccataaccc cgaacgcttc
    21061 aggctcatgg cgaacctcaa cccacacttg cacttcccgg tggtagacga gctggagcgc
    21121 cagatcgagc tcggcgcggt cgggctcaag gtccatcccg tacatgggtc cttcgccccc
    21181 aacgaccgta tgctctaccc ggcctacgcc tactgcgaaa gggaaggctt ccccgtcgtt
    21241 ttccactgcg gcacaagtgt cttccccggt tcgtcgaacc gctacgccga ccctgccctg
    21301 atcgaggacg tcgcccgcga cttccccgac ctcaccatgg tcctcgccca cggcgggcgc
    21361 ggctggtggt acgacgccgc agcctttatc acgctcatgc gccccaacgt ctggatcgag
    21421 gtctccggac tccccccaca taagctcccc gattattaca ggaacttcga cttcgagcgc
    21481 ctcgcccgga agatgctctt cggcaccgac tggcccggcg tgccaggccc aggctccaac
    21541 gcccaggcta tcgccaacct cgatctcgaa cgcgagacac tggagaggat cttctacaag
    21601 aacgccctcc gcgtttacga aagaatcgga gcgggtaacc tcacaataat tgatattgac
    21661 tgaccggaca gttattatgc tgcagccggt tagacagagg aggaaggcat gaaggcggta
    21721 actgaagaga tactcctgga tcgcatcaag agcgggaggc tcatcgagga ctcttcgcag
    21781 gcgacggaga ggtacgtcga gggtttgaag cggactctca tcgtctccgc cgacaccgag
    21841 cttataagtg ctccggcata catgaacgcg acgaaagact tctcgacgct cccaagcgtc
    21901 aacaactaca tcaccgtcat ggggatagtg caggacgagc tcgggcacgc gcacatcgcc
    21961 taccgcatgc tgagagatct cggggtggac acggaggagc tcgtctacga gcgcaagccg
    22021 aggcagttca agtaccctta tgccttcgat gtgccgcttg agagcttcac ggagctcatc
    22081 gtcgccaacg ggttttacga cagggccggc ttcgtactgc tgagtgacat ccaccacaac
    22141 tgttcatacg ggccgtggaa gagggctctc gtcaaggtgg accgcgagga gaacttccac
    22201 ctcaggcacg gtgagaagtg gatgaaggcg ttctccaggg acccggagaa gaaggaggag
    22261 ctccagcggg cggtcgagtg gatgttcccg ctgacgctcg agtggttcgg cctgcctgac
    22321 ggacagaagc ggcacaacga gcagatcgag tacggttaca agggctcgac caacgaccag
    22381 ctcaggcaga cctggatgag ccacacggtg cccctgtgcg aatcccttgg gctcgacgtg
    22441 ccggcccact acgacgagca agaggacacg tacttcatag attccccctt cccggcccag
    22501 ttcgacgctg aagagaagcg gtgggagttc gaggagggtg agatctcctg ggacgacgta
    22561 ctcgtccgct ggagggaacg cggtcccaag aacgaggagt acgtcgagca tcttcagcgc
    22621 ggctacaagg agctctatgc cagggaggcc gtctagcgat gcaggagaag atcatcgctg
    22681 cgggcgtcac agcggaggag gcgcgggagg cgctcagaga ggtgttcgac ccggagtacc
    22741 cgatcagcct cgtcgacctc ggtctcatcc ggggcgtcga ggtggaaggg tcgaaggcga
    22801 agataaagct gacgtacacc tgcatgggtt gtccggcgat ggacatgatc caggacgacg
    22861 tccgcgacag gctcctcgag atggacggca tagacgaggt ggccatcgag gtagtgtggg
    22921 agacctggtc gaggaaagat attacgccgc ttgggaggaa gcaactcaag gacgtggggg
    22981 tagtctgatg cggccggtcg agaactacga ggtatttacg gctcgtgtgg gcgaagcccc
    23041 gctgaggcac aacggcaacg tcagggcagc ggagcccaga gacgcggccg tctatgcgca
    23101 cctcatgtac gacgagtggc gctggcgcga gatgttcgtc gtgccgagcc gggccatcgt
    23161 cagggttatc aggccggagt agggaggagt ggtggagaga tacatggtct tcgcgcgcaa
    23221 cgagtacgac gagccgctgg agcacagggg cgacgtcgag gccgccggta acgatgatgc
    23281 ggcgaagctc gccaaggagc gctacgggca agactggctc gagatgtcgc tcgtgcctgt
    23341 gagcaaggct tattgggccg agagggagac cgaagtgggc gaaacggagg tacaggtgtg
    23401 agcaagcgtc ccgaaggcac ggagcaagta gcgccgggac gtggggcgca gagcgagttc
    23461 ctcgtcgaga acgacgatgc cctggctgcg ctggtcaact tgatcgcggt cataggcgac
    23521 aacgtgtact tcctgggtcg acgcgtctcc gagtgggccg atgccgggcc gctcctggaa
    23581 tcgaccgccg cttgcgcggc cataacccag gacaagctag ggcaatcgcg ggcgatctac
    23641 cctctgctcg aagacctgcc ctggcctaac ccaccaacgg ggctgcaggg tgaggtcgac
    23701 agggcacgcc gttactctgt cagtttcctc gacgacccgc ttccaagctg gacgaacgtc
    23761 gtcgcagcgc tcgcgctcgt cggtcccgcg gtgaacactg tgctggaggc cgtggagcac
    23821 tcccgctacg aggcgctcgc aaggagggcg caccgcatcc tcgacgagga gaggctcacc
    23881 tctgcatacg ccgagagcct ggtacgccag atcgcctacg aggagcgggg ccgcaacctc
    23941 ttccaggttc gcgtcgacga gctcctgccc gagatgctct gctggttcgg ccccgacgga
    24001 gaggagggca tagaagccct cagggccgag ggcctgatcg acatgggcaa cgaggagatg
    24061 cgccagaggt atctgggcaa ggtcgttccg ctgctgcagg agtgcacaat agaagtcccg
    24121 atcgagtgga gtgagagcga aaggcgatgg gagtacggag agctgccatg gagagaatgg
    24181 aaccagctgc agagacgcct ggcgagatca gcgcagagga gcggcgtttg atctgccctt
    24241 ggtgcgactc tggcgaggtc gagaaggtct ccgagtacgg tcctcacttg atggtgtact
    24301 catacatctg ccgcagctgc aacagcccgt tcgaggtcat caaacgatga ccgagagttc
    24361 gagcttgcga accacgctag atgacagcgc gctgctcgtg gagttgaacc gtcccgagaa
    24421 gaggaacgcc ctcgacgaga cgctgcaggg cgagttgctg caagtattca cggacgcagc
    24481 gcacgaccgc aaggtgcgag gagtaatcct gaccgccgcc ggcgaggcgt tctccgcggg
    24541 cggtgacctc tctcgcttcg agcgcgattg ggaacaggcc gagtttcgcg cccacagcca
    24601 ggagttgacc cacctcatct cgtccatcga acggctcgag aagccggtca tcgcggccat
    24661 agggggcctt gcaaccggcg cagggaccca gctagccctc tcgtgcgacc tgcgcatcgc
    24721 ctctgaaaat gcccgcttcg tgttcaggga gggcatgatc ggcttgatcc cgagccatgg
    24781 cggatgcgtg cgcctggtca agctggtggg tctcgcgcgg gcgcgggaca tagtgctcgg
    24841 cggccaggat ctggacgcgg aggaagccta caggcacggg ctcgtcacag agatagtgcc
    24901 acacgagaag ctgttggacg aggcgaagga gcgtctacgg ttcatcctca agcgtgcgcc
    24961 acaggcttat ggactcagca agcggttgct tcatttctcg accagcgtcg acatagagag
    25021 cggtctgttc gcggagagca tggcgcagag cttgctcgtc ggcacggaag accacacaga
    25081 gggcgtgcgg gcagcccgtg agcggcgcgc gccctcgttc accgggaggt agctggatgg
    25141 gttatctact cccgttcgag gacaagaggc ctctggtggc cgacgacgcc tttatcgcgc
    25201 cgaccgcggt actaatcggg gacgttgtgg tcgaagaagg ggcgagcgtc tggttcggtg
    25261 cggtgctgag gggagacttc aaccggatcg tcgttgggtc agggacgagc gtgcaggaca
    25321 actcggtcat acacacgaac gaggaactgc cgaccataat tggttccaac gtcacggtcg
    25381 gccacctctc gctgctggag ggatgtgaga tcgaggacgg agcgctgatc gggatgggga
    25441 gcatcgtctt gaaccgggcg cgtgtc
//
LOCUS       DASTKM010000202         4134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_938376, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000202 DASTKM010000000
VERSION     DASTKM010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938376"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFQ10_08425"
     CDS             <1..233
                     /locus_tag="VFQ10_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965407.1"
                     /translation="IRAFNARIAPERAATARPPKPMVRICESCGREIKVERGEQSHPV
                     WQKNGVIRMFCTLPMCDLDARVVEEEEERAKA"
     gene            complement(1423..3402)
                     /gene="ligA"
                     /locus_tag="VFQ10_08430"
     CDS             complement(1423..3402)
                     /gene="ligA"
                     /locus_tag="VFQ10_08430"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET9965408.1"
                     /translation="MDVRGRVEDLREQVRYHNRRYHVDDAPEISDAEYDALYRELEAL
                     EDEHPELITPDSPTQRVGGEPIEGFEEVRHSVPMLSLANARKIEDLREWDARVRRLLG
                     EDETPRYVTELKIDGLAVSLRYENKRLVMGATRGNGVLGEDVTAQLRTIRSIPERLDD
                     TPPEVLEPRGEAYIPIADFEKLNRELEADGKKPYANPRNLAAGSIRQLDPKITARRPL
                     TILLYGVGEGGEIFESHSEMLAALKAYGLRVNPYEVHQDIDSVIEECERWAKERESIG
                     YQIDGVVVKVDSREQQLALGSVARSPRWAIAYKFEPLAGRTKLLDVRVKVGRTGALTP
                     QAVLEPVNVGGVVISRATLHNEDYVKEKGILIGDTVVVERAGDVIPQVVRAVTGKRKG
                     DEYAFEMPANCPVCDEPVSRPEGEAVTRCVNARCPAQALEHIIHWSSKSAMDIDGLGE
                     KLATRLFDLGFISDVADVYDLEAEQLIPLEGFGEKSAGNLIQAIERSKDRPFSLVLYA
                     LGIRHVGSVTASLIATRFGGDDLMRGVEVEQLSEIDGVGEVVARAVVEYFDLEDNRDL
                     VRRLMERGLDFGRQVGGPPEGPLSGKRVVITGTLDRSRNYYVGRLEAAGGTFTSSVSK
                     NTDYVLAGEDAGSKLERAKELGVPVIDEVGFEELF"
     gene            complement(3706..4011)
                     /locus_tag="VFQ10_08435"
     CDS             complement(3706..4011)
                     /locus_tag="VFQ10_08435"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9965409.1"
                     /translation="MESANHRPDNPYTQLEGYRVHDSSGEEVGEIEDTVYDAPSDVLK
                     YVVVRGRPIPAERIEVDAGDQRVSVPYDARTIESAPQMEDPSGAFDEAVHEHYEGRA"
BASE COUNT          773 a         1300 c         1230 g          831 t
ORIGIN      
        1 acataagggc ctttaacgca aggatagcac cagagcgggc agccacggca aggccaccaa
       61 agccgatggt acgtatatgc gagtcgtgcg ggcgggagat caaagtagag aggggcgagc
      121 aatctcaccc cgtgtggcag aagaacggcg taataaggat gttttgcacg ctgcctatgt
      181 gcgacctcga cgcgcgggtg gtagaagagg aagaagagcg ggcaaaggcg tgacttctgg
      241 agaacctaag ccgcatattc gggacattac gatgcgccgt ctaacgccac aagagcacct
      301 cccagggggc gcatcgggag ccgtgagtga ccgctggcgc gcgatcgcgt gccccaatgc
      361 accccctggg aggcccctat tcatccgaca acttagaagg cgccttctcc gaagtagtgc
      421 cagcctcgcc tcatttacta caatggatga agggatagca ccgtggggag aagaagggct
      481 tacataagga ggagcaggtg ggatgtcgtg agagttgctg tgggggcgct tgtcggattc
      541 ctcataggat tcctcgtcgg attcctcgtc gccgggctca tcggtggaaa ccaagagggg
      601 gagcaggccg ctccgagtgg gcctcgtacc gtgatagttg agaagaccgt agagaagacc
      661 attccggcgg ctaccccgga gcctaggtcc ccaaataatc ctccagagca agataagccc
      721 ccagatacgc ccacagctac agctacagct acagctacag ctacagctac agctacagct
      781 acagctacag ctacagctac agctacagct acagctacag cctctccctg atcatcaagc
      841 gaaaccttgg ggcgctttcc tctccatatt caccgtagaa gctgtgcgag aagggtcagg
      901 cgcgggcgag cctcctcaac atcaggcggt tcatgaccgc atagaccatg gtggcctgcc
      961 tactctcgca caatctctcg taatccctaa gctcatcctc ctgctcaaag caagccaacc
     1021 aatggttcga tccgcctccc agcgtctcgg tagtgggccg cggaatctct tcggttcagc
     1081 tggaagcctg acccacccca accagacggt cgaggggtcg ttgagatcgc cgcgtggttg
     1141 ctactcgccc gcggctgacc cttctcgcac agcttctagt ggaggcatta gttgagcgtt
     1201 gccaagttct gagcgagtag ccggagatca tcggctactc catctattat aactggtggc
     1261 cgaaccaacg ctattcaaac ggacttggta tgacacgctg ataggcggcg aagccgccgt
     1321 cccgaagtgc tgacaagcga aggcctttca ggcctgagcg tgctgactag ctgatctact
     1381 gaccagcgcg ggttttggaa ggctcaaccc ctccaaaacc cgctaaaaca gctcttcgaa
     1441 cccgacttcg tcgatcacgg gaacgcccag ttcctttgcc ctctccagct tcgagcccgc
     1501 atcttcgccg gcgagcacgt agtctgtgtt cttgctgacc gaggaggtga aggtgccccc
     1561 tgccgcttcg agacggccta cgtagtagtt gcgggaacga tccagggttc ctgttatgac
     1621 gacgcgcttg cccgagagcg gaccctctgg ggggccgccc acttggcgtc cgaagtcgag
     1681 gccccgctcc ataaggcgtc ttacgagatc gcggttgtct tcgaggtcga agtactccac
     1741 gacggctcgg gctacgacct ctccgacgcc gtcgatctct gaaagctgtt cgacttcgac
     1801 gccccgcata aggtcatctc caccgaagcg cgtagcgata agactggccg taaccgaccc
     1861 tacgtgcctg atgccgaggg cgtagaggac gagggagaac ggcctgtcct tactcctctc
     1921 gatggcctgg atcaaattcc ccgcgctctt ctccccgaag ccttcgagcg ggatgagctg
     1981 ttcggcttcg aggtcgtaga cgtcggccac gtcgctaata aagccgaggt cgaacagccg
     2041 ggtggcgagc ttctcgccca ggccgtcgat atccatagcg gacttcgagg accagtggat
     2101 gatgtgctcc agagcctgcg cggggcaacg ggcgttcacg caacgggtaa cggcctctcc
     2161 ctcggggcgc gagaccggct cgtcgcagac agggcaattg gctggcatct cgaaggcgta
     2221 ttcgtcgcct ttgcgtttcc ctgtgacggc tctgacgacc tgcggtatca catcccccgc
     2281 ccgttcgacg acgaccgtgt cgccgattag gatgcctttc tccttgacgt agtcctcgtt
     2341 gtggagcgtc gcccgtgaga taacgacccc gccgacgttt acgggctcga gcaccgcctg
     2401 cggcgtcagt gcgccggtcc tgccgacttt cacgcgtacg tcgagcaact tcgtgcgtcc
     2461 tgcgagcggc tcgaacttgt acgctattgc ccaccgcggc gaccgggcta cagagccgag
     2521 ggcgagttgc tgctcgcgcg agtcgacctt caccacgacg ccgtcgatct ggtagccgat
     2581 ggactcccgc tccttcgccc accgttcaca ctcctcgatc acggagtcta tgtcctggtg
     2641 gacctcgtag gggttgacgc gcagcccgta tgcctttagc gcggcgagca tctccgagtg
     2701 gctctcgaag atctcacccc cctccccgac gccgtagagc agtatggtga gaggccttcg
     2761 ggccgtgatc ttcgggtcga gctgcctgat ggagccggcc gccaggttgc gcggattggc
     2821 gtatggtttc ttgccgtctg cttcgagctc cctgttcagc ttctcgaagt cagcgatggg
     2881 gatgtacgct tcaccgcgcg gctcgagtac ttcgggcgga gtgtcatcga ggcgctcggg
     2941 gatcgaacga atcgtcctta gctgcgccgt gacgtcctct ccgaggacgc cgttgccgcg
     3001 tgtggcgccc atcaccagcc gcttgttttc gtagcgcagc gagaccgcca ggccgtcaat
     3061 cttcagctcc gtcacgtagc gcggggtctc gtcctcgccg agcagacgcc gcacgcgggc
     3121 gtcccactcc ctcaggtctt ctatcttgcg ggcgttcgcg agggagagca tggggacgga
     3181 gtgcctgacc tcctcgaatc cctcgatggg ctcgccgccg acgcgctggg tcggtgagtc
     3241 aggggtgatg agttcgggat gctcgtcctc cagggcttcc agctccctgt agagagcgtc
     3301 gtactcggcg tcggagatct ccggcgcgtc atcgacgtgg tagcggcggt tgtggtaacg
     3361 gacctgctcg cgcagatcct ctaccctacc ccgaacgtcc acgcctccgg ccacgcacga
     3421 attatacgca cctacgcacc cgctcccaag agaagcttga tcgccaccgt gccggacagg
     3481 gcctggtaag gcttccgatc tggtctaacg aatacacaag aggacccggg cacagattcg
     3541 gcccctcctt ttggtttgat ctctcctctg gctaccacgc atctcgaaag gaacgggacc
     3601 cggctagcaa tgtgctacgt tttcggccta cgaatcgcgc cctcaaccct gctgatgcct
     3661 gaagccgcgg tttacaggag gaccactgtc ctcctgtaaa ccgcactaag cgcggccctc
     3721 atagtgctcg tggacggctt cgtcgaaagc tcccgaaggg tcctccatct gtggtgctga
     3781 ctctatggtc ctcgcgtcgt aggggacaga gacgcgttgg tctccggcat ctacctctat
     3841 cctctcggct gggatggggc gtcccctgac gacgacgtac ttgagcacgt cgctcggggc
     3901 gtcgtagacg gtgtcttcga tctccccgac ctcctccccc gaggagtcgt ggaccctgta
     3961 gccctccaac tgcgtgtagg ggttatcggg gcgatggttt gcactctcca tacttcatcc
     4021 tccggtggcg ggcatacgag tatgtgcgta tgatacttcg gcgtgggcgg cagaacagat
     4081 caaggccgga acgcccggag cctgaaggct ttctcattcc tgctcctggc cctc
//
LOCUS       DASTKM010000203         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_938796, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000203 DASTKM010000000
VERSION     DASTKM010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938796"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VFQ10_08440"
     CDS             complement(<1..1041)
                     /locus_tag="VFQ10_08440"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HET9965410.1"
                     /translation="MRRSSSVVAALTLLAFLAGACGALGGDGRSGGTRSADRPAAALG
                     PEDEPPEGAEAEFATDFSRHSVPYSEILSGGPPKDGIPAINDPRFVGIEAADSWLEPK
                     EPIILFQIGDDVRAYPIQIVMWHEIVNDMVGGVPVVVTFCPLCNTAIAFERVMDGREL
                     TFGTTGRLRFSNLIMYDRQTESWWQQATGEAIAGKFTGKRLDFRPASIVSWADFRSAH
                     PDGKVLSRETGFSRSYGQNPYAGYDDVDNSPFLYEGPRTLGTLPPMARVITTESGSEA
                     VAYPYELLRQERVVNDTVGGEPVVVLWKPGTASALDDDAVAAGRDVGSAAVFSRTLDG
                     ATLTFRSEGGRIV"
     gene            1296..2714
                     /locus_tag="VFQ10_08445"
     CDS             1296..2714
                     /locus_tag="VFQ10_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HET9965411.1"
                     /translation="MRGHHYRGRDSLSHSAPREAPAKLALKANDVLRRNDMGGWTKAA
                     PELYPHQWSWDSAFIAIGLAHLDTRRAARELETLFAHQWKTGKVPHIVFNQEAPPESY
                     FPGPEHWACAAASPDAPPAPPYTSCLSQPPVHAIAALRIWEAAQRGSEDEILAANSFL
                     KGIYPRLLSWHRYLLTYRDPERSGLVTIYHPWGSGTDNSPRWDAPLEALEVGDVGPYP
                     RHALQHVGSPSQRPTNAEYDRYLWLVNLIKRAGCDEATIYETHPFLVKDVLTSAILVA
                     ANEALLEIADLVGAPEEEQGLIAEWMARSRQGLEGCWDAEFGSCLDYDVRADRSLRVR
                     TVAGFAPLIAGGLSRERLRALLETLNSPAFVGHRGLRWPLPPSTSPEDSRFKPRSYWR
                     GPTWPVVNWLFWWSLLRVGEHERAEELRRTALDQLAVRGFGEYFDPFTGQLLGSADQS
                     WTAAVALDWLAHEPAPEENLPS"
     gene            2711..2851
                     /locus_tag="VFQ10_08450"
     CDS             2711..2851
                     /locus_tag="VFQ10_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965412.1"
                     /translation="MRIRIRWDRASSTQTSGAKRPFTIGMVILFILAAAPMLEEVILN
                     VR"
     gene            2912..>4203
                     /locus_tag="VFQ10_08455"
     CDS             2912..>4203
                     /locus_tag="VFQ10_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HET9965413.1"
                     /translation="MTETHHYDAVVIGSGQGGNPLATTLAKAGHKAAIVERQHVGGTC
                     INYGCTPTKTMVASAKAAYMARRSADYGVKSGPVSVDMVKVRQRKRYIVESFRGGSEG
                     RLESAGNLDVLMGEASFTGPKELKVRLNAGETLQFTADLIVINTGARPRRPPVEGLDG
                     VPTLNSNTIMELDEVPDHLLVIGGGYVGLEFAQMFRRFGSEVTIMQQGDQLLRREDPD
                     VAEAVTEILREDGIGVLLETQAQRAEQTEEGRIQLTLSTPGGERKLSGSHLLVAAGRV
                     PNTEPLNLSAAGVKTDEGGFVLVDDKLETSVEGIYALGDVKGGPAFTHISYDDHRVLK
                     TNLIGGGEAAISDRLVPYTVFIDPQLGRAGVSEREAREQGRSVKVAKMPMSYVARALE
                     VDEPRGMMKAVVDADTDQILGCAVLGIEGGEIAAMIQIA"
BASE COUNT          818 a         1317 c         1332 g          736 t
ORIGIN      
        1 gactatccta ccgccttcgg aacggaaggt gagggttgcc ccatcaaggg tgcgggagaa
       61 gactgcagcc gaaccgacgt cgcgacccgc cgctaccgcg tcgtcgtcca atgcggaggc
      121 agtgcccggc ttccagagca ccacgacagg ctccccgccc acggtgtcgt tgaccacccg
      181 ctcctgccgc aggagttcgt aagggtaggc gacggcctcg ctacccgact cggtggttat
      241 cacgcgagcc atcggtggca gggtacccag cgttcttggt ccctcatata ggaacggcga
      301 gttgtcaacg tcgtcgtagc ccgcgtacgg attctggccg taggaccgcg agaagcccgt
      361 ctcacgcgag agcaccttac cgtccgggtg cgcagacctg aagtcagccc aggaaactat
      421 tgacgccgga cggaaatcca gtcgcttgcc cgtaaatttc ccggctatag cctccccggt
      481 tgcttgctgc caccagctct cggtctggcg gtcgtacatg atcaggttgg aaaagcgcaa
      541 cctccccgtg gtgccgaacg tgagttctcg cccgtccatg accctctcaa aggcgatggc
      601 ggtgttgcat agcgggcaga acgtcaccac caccggcacc ccgcccacca tgtcgttgac
      661 gatctcgtgc cacatgacga tctgaatggg gtaggcccgg acgtcgtctc ctatctggaa
      721 caggataatc ggttccttcg gttctagcca ggagtcagcc gcctcgatgc caacgaagcg
      781 ggggtcgttg atggcgggaa tgccgtcctt gggcggaccg ccggagagta tctcggagta
      841 ggggacactg tgcctggaga agtcggtggc gaactcggcc tcggccccct ccgggggctc
      901 gtcctctggt ccgagtgccg cggcgggccg gtctgcagac cgcgtgccgc cagagcgtcc
      961 gtctccgccc agggcaccgc aagctcccgc gaggaaagcg aggagggtca gggccgccac
     1021 aaccgatgat gagcgcctca tcctgctcct cctcgtccat tcaaccctct tatcttgcat
     1081 gggcgtaacc ccgatgtaag tgacgtacac caccaggaga ctgcgccacc tcctactgcc
     1141 tccgactacg cagcgcctac ttttattacc tgtgtcgcgt caacgaagta agagatagcc
     1201 ctggcggata gtcgatatcc ctacagagtg ccggaggcaa gatgaaacac tagggcacca
     1261 ggtttaggaa ggtactggtc taggtggagg aaggcgtgag aggccaccat tacaggggga
     1321 gggacagtct ctcccacagc gctcctcgcg aggcgccggc gaaactcgcc ttgaaggcga
     1381 acgacgtact gcgccgcaac gatatgggag gatggaccaa ggccgctccc gaactctatc
     1441 ctcaccagtg gagttgggac tccgcgttta ttgccatcgg cctcgcccat ctcgacaccc
     1501 gccgcgcggc ccgagagctc gagacgctct tcgcccacca gtggaagacc ggcaaggtac
     1561 ctcacatagt cttcaaccag gaagccccgc ccgagagcta cttcccgggg ccggagcact
     1621 gggcctgcgc tgcagcctcc cccgacgctc cgcccgcgcc gccctacact agctgcctca
     1681 gccagccgcc ggtacacgcc atcgcggcgc ttcgcatctg ggaagccgcc cagcgtggga
     1741 gtgaggatga gatcctcgct gcgaattctt ttctgaaggg catttacccc aggctcctct
     1801 cctggcaccg gtatctcctg acctacaggg acccggagag gtccgggctc gtaaccatct
     1861 accacccgtg ggggagcggt acagataact ccccgcggtg ggacgcgccc ctggaagctc
     1921 tggaggtagg agacgtagga ccttatcctc gccacgcctt gcagcacgtt ggctccccct
     1981 cgcagagacc caccaacgcc gagtacgatc gttatctatg gctggtgaac ctaataaagc
     2041 gcgccggctg cgacgaggcc accatttacg agacacaccc gttcctggtc aaggacgtcc
     2101 tcaccagtgc cattctggtg gcggcgaacg aggccctgct ggagatagcc gacctggtgg
     2161 gggcacccga ggaagagcaa ggcctgattg cggaatggat ggcgcgcagt cggcaaggac
     2221 tagaggggtg ctgggacgcc gagttcgggt cctgtctcga ctatgacgtg agggcggaca
     2281 ggtctcttcg ggtgcgcacg gtcgcgggct tcgctcccct gattgcgggc ggtctgagcc
     2341 gggagcgact ccgtgctctc ctcgagaccc tcaattcacc ggcgtttgtc ggccacaggg
     2401 gcctgcgctg gcctctgccg ccctccacca gtcccgagga ttcacgcttc aagccgcgca
     2461 gctactggcg cggaccgacg tggccggtgg tcaactggct cttctggtgg tctctccttc
     2521 gcgtcggcga gcacgagagg gccgaggagc tacgacggac ggccctggac cagctcgccg
     2581 tccggggctt cggggagtac ttcgacccct tcaccggcca actcctcggc tccgccgacc
     2641 agtcgtggac tgccgccgta gccctagact ggctggccca cgagccggcc ccggaggaga
     2701 atcttccttc gtgagaatcc gcatccgttg ggaccgggcc tcatcgactc agacatccgg
     2761 cgcgaagaga ccgtttacaa tcggtatggt aatcctcttc atccttgccg cggccccgat
     2821 gctggaagag gtgatcctga acgtaagatg aagcgagcca ctgtagtcaa tcagaacggc
     2881 acgctacaaa tctatgaagg gagccttatc aatgaccgaa actcaccact acgacgccgt
     2941 tgttatcggg agcgggcagg gcggcaaccc gctcgcaact acgcttgcga aggcggggca
     3001 caaggccgca atcgtggagc gtcagcatgt ggggggcacc tgcatcaact acggctgtac
     3061 cccgacgaag accatggtcg ccagcgcgaa ggccgcatac atggcccgtc gttcggccga
     3121 ctacggtgta aagagtggcc cggtaagtgt ggacatggtc aaggtgcgtc agcgcaaacg
     3181 ctatatcgtg gagagcttcc gtggcgggag cgaaggccgc ctcgagagcg cagggaacct
     3241 tgatgtgctg atgggcgaag cctcattcac tggtccgaaa gagctcaagg tacgcctgaa
     3301 tgcgggcgaa acgctccagt tcaccgccga cctcatcgtc ataaacactg gagcccggcc
     3361 cagaaggcca cctgtagaag ggctcgacgg tgtgccgacg ctgaactcta atacgatcat
     3421 ggagttggac gaggttccgg atcacctgtt agtcattggt ggcgggtacg tgggcctgga
     3481 gttcgctcag atgttccgtc gctttggcag cgaggtcacc atcatgcaac agggcgatca
     3541 gctcctgaga cgcgaggatc ccgacgtggc cgaggccgta acggagatcc tacgcgagga
     3601 cggcattggg gtgctgctcg agacccaggc ccagcgtgcc gagcagacgg aggaggggag
     3661 gatccaactg accctcagca cgcccggagg agaacgcaag ctctccggtt cacatctgct
     3721 ggtggccgcc gggcgggtgc ccaacaccga gccgctcaat ctgtccgcag caggcgtcaa
     3781 gacggacgag ggaggtttcg tgctggtaga cgataagctg gagactagcg tagagggtat
     3841 ctacgccttg ggcgacgtga agggcggccc cgccttcacc cacatctcct acgacgacca
     3901 ccgcgtcctc aagaccaacc ttataggggg cggcgaagcc gctatctcag accgccttgt
     3961 accctacacg gtgtttatcg acccgcagtt gggccgtgcg ggcgtcagcg aacgggaggc
     4021 ccgtgagcag ggtcgcagcg tcaaagtagc caagatgccg atgagctatg tggcgcgggc
     4081 gttggaggtg gatgagccgc ggggaatgat gaaggccgtg gtggatgccg atacggatca
     4141 gatcctgggc tgcgcagttt tgggaataga aggtggcgag atagctgcca tgatccagat
     4201 agc
//
LOCUS       DASTKM010000204         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_56998, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000204 DASTKM010000000
VERSION     DASTKM010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56998"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFQ10_08460"
     CDS             complement(<1..237)
                     /locus_tag="VFQ10_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965414.1"
                     /translation="MKDGHPIFAALYDQKSASAERRWMGERRRRLLAGARGAVLEIGG
                     GTGANLAHYRNVDRVTIAEPDPFMRNRIGPKLEEA"
     gene            473..910
                     /locus_tag="VFQ10_08465"
     CDS             473..910
                     /locus_tag="VFQ10_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET9965415.1"
                     /translation="MEAKDHNYGVAGDEVAKEQGPQMMGTQEEKTMSVLAHLSIFLNL
                     VTGFLGPVAALIIWLVYRDRSSNVGFQALQSMWYQVAWLVILVAGWTVTTLLMLVLIG
                     FLLVPVMALITLVPFVHSAYAAYRVSKDGGYRYPLIADMIEAR"
     gene            1050..1355
                     /locus_tag="VFQ10_08470"
     CDS             1050..1355
                     /locus_tag="VFQ10_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965416.1"
                     /translation="MLDSRESSPPVAVPDPVSVTGSRDVTELLLGVLRSIEPYEACIL
                     EAEQDGDFEVAAFLRELRRQDLIRAREATRLLKRSLEAAHINVRKVGEPYVELSARP"
     gene            complement(1422..1805)
                     /locus_tag="VFQ10_08475"
     CDS             complement(1422..1805)
                     /locus_tag="VFQ10_08475"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HET9965417.1"
                     /translation="MSLFEYALLRVVPRVERGEFINAGVVLYCQDAKFLDARIRLDPE
                     RLRALDPNLDPEIVQAHLEVARRVCEGGAEAGAVGMLPQGQRFGWLIAPRSTVVQPSP
                     VHTGFADDPEEAIEHLLKVMVETPE"
     gene            complement(1802..>2657)
                     /locus_tag="VFQ10_08480"
     CDS             complement(1802..>2657)
                     /locus_tag="VFQ10_08480"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HET9965418.1"
                     /translation="TGSVVEGTDGCDNGAAVRPLDRDLLRTVTATRYVTPLREGGSLP
                     AIVEADDLGTYVLKFRGAGQGHKALVAELVAGEIARRLDLLVPEVVLAYLAPELGRSE
                     RDPEIQDLLNRSTGLNLALDYLPGSLGFDPLVEPPDSDLASHIVWFDALVTNVDRTPR
                     NPNLLVWHDRLWLIDHGASLYFHHNWPARDGTALEATSRRPFAAVRDHVLLPFADSIS
                     EADAALAPRLTLEVLRSIVDMIPEEWLAGESGFGSVEEVRFAYLAYLTTRLREPRGWV
                     SALEETTR"
BASE COUNT          496 a          802 c          869 g          490 t
ORIGIN      
        1 ggcttcctcg agcttcgggc cgatcctatt acgcatgaaa ggatcgggct cggcgatggt
       61 tacccgatct acattacggt agtgcgccag gttagcgcct gtgccgccac cgatctcgag
      121 cacagcgcca cgggcaccgg cgagcaatcg cctcctgcgc tcgcccatcc accttctctc
      181 cgcggaggcg cttttctggt cgtagagcgc agcgaagatg ggatgtccgt ctttcattga
      241 actggtttcg tgatggtcgc gcgatctccc tatgggcact ccacccagag tctgacacgt
      301 cagaagcgac ttcgccatct gatagcgtcc aacgatcaag aagctgactt gctgacaagc
      361 cgaaacgctg ggcccgcgga cctagcaaca ttcagtcctg ggaccgatgc cgaatcggtg
      421 gtcagggagt accttgctta tgtaagtcgt agcgtgacag agaggagtgg cgatggaggc
      481 gaaggaccac aactacgggg tagccggaga cgaggtagcc aaggagcagg ggccccagat
      541 gatgggcacg caagaggaga agacgatgtc ggtgctcgcg caccttagca tcttcctcaa
      601 cctggtcacc gggttcctcg ggcccgtggc tgccctgatc atctggctgg tctatagaga
      661 ccggtcgagc aacgtgggtt tccaggcttt gcagtcgatg tggtaccagg tggcttggct
      721 tgtgatcctg gtcgcgggat ggaccgtgac gacgctcttg atgctggtct tgatcgggtt
      781 cctgctcgtc cccgtgatgg ccttgatcac gctggtgccc tttgtgcaca gcgcttatgc
      841 ggcctaccga gtgagcaagg acggcgggta ccgctacccg ttgatcgccg acatgatcga
      901 agcccgttag aaggcagtca gagagaaccg acgcccccga ctcgtatggg ttgggggcgt
      961 cgctccagga ggattttcat ccggttctga cactattcgt acatgtcgtg cgtatacgta
     1021 agaaacgtgg atactcgagg aggatagaga tgctggacag cagggaaagt tctccaccgg
     1081 tggcagtccc tgatcccgtg tcggttacag ggagtcgtga cgtgacggag ctcctgctcg
     1141 gcgtgttgag gagcatcgag ccctacgagg cgtgtatcct cgaggccgaa caggacgggg
     1201 acttcgaggt cgccgccttc ctgcgcgagt tacggcgcca ggacctcatc cgggcgaggg
     1261 aagcgacccg cctactaaag agatccctcg aagcggctca cataaacgtc aggaaggtgg
     1321 gagagccgta tgtggagctc tccgccagac cttagcttgg aggtcgtccc actcataacg
     1381 cccatgaggg tgccgggtcg cgcacgccgg cgactgggga tctactcggg cgtctcgacc
     1441 ataaccttca ggaggtgctc tatggcctcc tcggggtcat cagcgaagcc cgtatgcact
     1501 ggagaaggtt gcaccacggt gctgcggggc gcgatgagcc atccgaagcg ctggccctga
     1561 ggcagcatcc ccacggcgcc ggcctcagct ccgccttcgc acacccggcg cgcgacctcg
     1621 aggtgtgcct gtacgatctc ggggtcgagg ttggggtcga gcgcccgcag gcgttccggg
     1681 tcgaggcgga tgcgcgcgtc gaggaacttc gcgtcctggc agtagagtac cacccccgcg
     1741 tttatgaact caccgcgctc gacgcgtggc accacgcgca ggagtgcgta ctcgaaaagg
     1801 ctcatcgcgt agtctcctcc agcgcactta cccaccctct tggctcccgc aagcgggtag
     1861 ttagataggc gaggtatgcg aagcgcacct cttcgacgct cccgaaacct gactcgccag
     1921 cgagccactc ctcggggatc atgtctacga tggagcggag cacctccaag gtgagccgcg
     1981 gggcgagcgc cgcgtcggct tcggagatag agtcggcgaa agggagcaga acgtggtctc
     2041 tgaccgcggc gaaggggcgc cggctcgtgg cctccagggc tgtcccgtct ctcgccggcc
     2101 aattgtggtg aaagtacaac gaagccccgt ggtctatgag ccagagacgg tcgtgccaga
     2161 ccaggaggtt cgggttgcgc ggggtgcggt cgacgttggt caccagggcg tcgaaccaga
     2221 cgatgtgcga ggcgaggtcg gaatccggcg gctctacgag cgggtcgaat ccaagggagc
     2281 ctggcaggta gtccagcgcc aggttcaggc ccgtactcct attcagcagg tcctgtatct
     2341 cggggtcgcg ttcagagcgg cccaactcgg gcgcaaggta ggcaagcacg acctcgggca
     2401 cgaggagatc gaggcggcgg gcgatctcac ctgccaccaa ctccgccacc agcgctttgt
     2461 gaccctgtcc agccccgcgg aatttgagga cgtaggtccc gaggtcgtca gcctcgacga
     2521 tcgccggcag cgaacccccc tcacgcaagg gcgttacgta gcgtgtggcg gtcacggtgc
     2581 ggagcaagtc acggtccaat ggtcgaactg ccgcgccatt gtcgcacccg tccgtccctt
     2641 ccacaacgga ccccgta
//
LOCUS       DASTKM010000205         7232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_763937, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000205 DASTKM010000000
VERSION     DASTKM010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763937"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFQ10_08485"
     CDS             <1..798
                     /locus_tag="VFQ10_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965419.1"
                     /translation="GDELRRVFVTHTSGKDDSSWVKPFVLPLRGTTVSSDDISGIVAF
                     GSKIGTMWGNQYDESGKSGYYFAIHKDGEPDDTWRQDNPVMGADMANDHVNLKADSEG
                     RVFATLKTRRDRIDRDLDAPYSMLWVRDEVGNWTSHVFGTVEDSHTRSLALIDEEKRL
                     LYMFASSPTCSGGKVYYKRTDLDDISFEEGRGTLFMQGPKGKNVGDATSTKQNLDEAS
                     ELLVVASDKNRGYYYNLIDLRNQEKLFPHGARIRTDTAPSGAPEPRR"
     gene            802..1653
                     /gene="lgt"
                     /locus_tag="VFQ10_08490"
     CDS             802..1653
                     /gene="lgt"
                     /locus_tag="VFQ10_08490"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET9965420.1"
                     /translation="MIPSSQILLALFSLPSPPSPIIFKVGPIALSYHGLLIALGIAVG
                     TWITRRELARRGYDGTTLALGSLFFVVPLGILGGRLYHVATEYHRYTRNPFPKVLETY
                     DGGLGIYGAVAGGFLGMLLFCWYRGINALTFADAAAPGLALGQAIGRWGNYFNQELFG
                     QPSDLPWAIRIAPLHRPTEYADATSFHPSFLYESLWDVLVCLVLLWVARRFSERLKVG
                     DVFMLYVVLYSVGRFLVETLRVDPAFVIGGSVRGNLFVSGVLTLGFALLLLLRHSRSS
                     QGTKTYH"
     gene            complement(1961..2623)
                     /locus_tag="VFQ10_08495"
     CDS             complement(1961..2623)
                     /locus_tag="VFQ10_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9965421.1"
                     /translation="MAIVDDHELTRESLQNMLSDEPDIEIVGEAANGRQALSLGSRFR
                     LDLILMDVRMPVMDGLEATKEIKQRYPEISVMMLTMHENPDYLLGALKAGAAGYVLKD
                     APHEEIVEAVRKVRNGESPLDPELAARLLRRLASEGEVRRETGGTHAAPYAVEPLTPR
                     ELEVLGLMKLGRTNRQIAGDLVISLGTAKNHVEHIISKLGVSDRTRAVVQGLELGLID
                     LP"
     gene            complement(2761..3675)
                     /locus_tag="VFQ10_08500"
     CDS             complement(2761..3675)
                     /locus_tag="VFQ10_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9965422.1"
                     /translation="MLRILILEDCSLDAELVGARLSEGGIGCELVRVQSRDAFAAALE
                     EGGVDLILADYDIVSSLTSQAASALENAWLYKELDDRERALQNLAKSLLGAQEVERRR
                     VAYEVHDGLAQVAVAAHQNLQAFARRYAPETEQGRRELDRILGQVRTTVSDARRVIAN
                     LRPTALDDLGLAAAISLEVERLNEEGYQVDCEEHLGEERLPREMEIALFRVAQEALTN
                     VRKHAGTKWLKIELQRRDTEVYLEVRDFGRGFDPAALQTLGGRPGERVGFAGMRERVG
                     MLGGELEIQSRPEAGTFVAAIIPLMRTA"
     gene            complement(3720..3926)
                     /locus_tag="VFQ10_08505"
     CDS             complement(3720..3926)
                     /locus_tag="VFQ10_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965423.1"
                     /translation="MDDRDYSSTLLLVEGNQNDVESTLSASSEGRLDYRLLRAAYRSQ
                     TAEDPKARLLGVNDHPSSAVGRKL"
     gene            complement(5067..6059)
                     /gene="trxB"
                     /locus_tag="VFQ10_08510"
     CDS             complement(5067..6059)
                     /gene="trxB"
                     /locus_tag="VFQ10_08510"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET9965424.1"
                     /translation="MAHRAYDVVIIGSGPAGYTAALYAARANLKVLVFQGFESGGQLM
                     LTSDVENYPGYRDGVMGPEMMDDFEAQAARFGAEMRPENIERVDFSERPFKLWAEDED
                     EATLAKAVVIATGAKAKWLGLDSEQQLMGKGVSGCATCDAFFYRGKKVAVVGGGDTAM
                     EEAGVLAKFADEVFLIHRRDEFRASRIMLERARKNPKITFITDTVVEEILGKDAVEGV
                     RTRNTKTDEERTIDVSGFFTAIGHTPATSLFEGQVEMKEGGYIIQKENTMTSVPGVFA
                     AGDVSDTRYRQAVTAAGDGCRAAIDSERWLEEQGEAEGAEDPAVWTAEKDVANF"
     gene            complement(6161..>7232)
                     /locus_tag="VFQ10_08515"
     CDS             complement(6161..>7232)
                     /locus_tag="VFQ10_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET9965425.1"
                     /translation="AEAGADVAVASPGPFFVQDDVAAALAERGVTVYASSENDPEDAD
                     RELERVLDRAAGDREAVFIDDRAGLVRLAHTTRRNLLGHIHGASEETTSGVAKFRAME
                     QEGVLEFPVIAANDARCKYLFDNRYGTGQSTLTAIMQNTNLMVGGKRVVVLGYGWCGK
                     GIARYAAGLGARVSVCEVDPVRGLEAYADGFDVLPALDAAEIGEVFITATGSRDVLTA
                     AHFERMHDGAILANAGGVDVEIDVDGLAATATRAREVRSNVEEFTTSDGRRLHLVGGG
                     MVVNLTASDGHPVEIMDLTFAIQALCAYRLANEHASMENKVHLLPREIDDEVATMKLD
                     AVGMSVDALTPEQERFLAGWRE"
BASE COUNT         1318 a         2399 c         2077 g         1438 t
ORIGIN      
        1 ggcgacgagt tgcgccgcgt cttcgtaacc catacttcag ggaaggacga ctcgagctgg
       61 gtgaagccgt tcgtcctgcc gctacggggc acgaccgtga gctccgacga catctcgggg
      121 atcgttgcct tcggctccaa gatcggcact atgtggggca accagtacga cgaatcaggc
      181 aagtccggct attacttcgc catacacaag gatggagagc ccgacgacac gtggcggcag
      241 gacaatccgg ttatgggagc ggatatggca aacgatcacg tcaatctcaa ggccgactca
      301 gaggggcggg tcttcgccac tctaaagacc cggcgcgatc gcatagaccg ggatctcgac
      361 gcaccataca gcatgctgtg ggtgcgtgac gaagtcggga attggaccag ccacgtcttt
      421 ggcacggtgg aagactccca cacgcgctcg ctcgcactta tagacgaaga gaagcgcctg
      481 ctctacatgt tcgcctcgtc ccccacctgc agtggtggca aggtctacta caagcgcacg
      541 gacctggacg acatctcttt cgaggaaggc cgcgggactc tgttcatgca aggccctaaa
      601 gggaagaacg tcggcgacgc cacctccaca aagcagaacc tcgacgaggc atcagagctt
      661 ctggtggtgg catcggacaa aaaccgtggc tactactaca acctgatcga cctccgcaac
      721 caggagaagc tgtttccgca cggagctcgc ataagaaccg acacagcgcc ctccggggcg
      781 ccggagcctc ggcggtagaa ggtgatcccc tcgtctcaga tactcctggc actcttttcg
      841 ctgccgagcc cgccctcacc gatcatcttc aaggttggac ccatcgccct gagctaccat
      901 ggactcctta tcgcgcttgg catagccgtt ggtacctgga tcacgagacg agagctcgcg
      961 cgcaggggct acgacggcac caccctggcg ttgggctcac tcttcttcgt ggtccctttg
     1021 ggcatcctcg gagggcgcct ctaccacgtg gcaaccgagt accaccgata tacccgcaac
     1081 cccttcccca aggtcctgga gacctatgac gggggcctag ggatctacgg cgcggttgcg
     1141 ggcggcttcc ttggaatgct gctcttctgc tggtaccgcg gcataaacgc gctcaccttc
     1201 gctgacgctg ccgctccggg tctcgccctg ggccaggcca taggtcggtg gggcaactac
     1261 ttcaatcagg agctcttcgg gcaaccctcc gacctgccgt gggccatacg tatagcgccc
     1321 ctgcaccgtc ctacagagta cgctgatgcg acctcctttc acccctcctt cctctacgag
     1381 tcactatggg acgtgcttgt gtgcctggtc ctgctctggg tggcccgccg cttctcggag
     1441 cgacttaaag tcggcgacgt cttcatgctg tatgtggttc tctactccgt cggacgtttc
     1501 ctcgtagaga ccctccgcgt ggaccccgcg tttgtgatcg gaggctccgt ccgcggcaac
     1561 ctcttcgtaa gcggcgtcct caccctcggc ttcgccctgc tcctgctcct gagacactct
     1621 cgctcgtccc aaggcaccaa gacctaccac tagcggtgga taacttccgc ggggtgtcct
     1681 tgtaacctcc aacacccctt ccgaaattgc ctcgaattgt gccatcgaaa atcggtagaa
     1741 caaatctgtt tgccaggacg aatatacgca agagcttact gggtccaggc aataacggct
     1801 aacatggcgc cggatcgagg gcccaacccg cagccaccct gagtgcccga accggcaacg
     1861 ctcttactcc cggccattcg ggtcaattga cgcgcgagga acgcctttgt gactttcggc
     1921 cagggggcat acgtgcgcgc cagagcgccg acggctatcc tcaggggaga tcgatcaacc
     1981 ccaactccaa gccctggact acggccctgg tccggtcgga gacccccagc ttcgagatga
     2041 tgtgctcgac gtggttcttg gccgtaccga ggctaattac gaggtcccca gcgatctgac
     2101 ggttggtgcg tccaagcttc attagcccca ggacctccag ctcacgcggg gtcaggggct
     2161 ccaccgcgta aggggccgca tgagtaccgc ctgtttctct gcgcacttcc ccctcgctgg
     2221 ccaaccggcg cagaagccgg gcggccaact ccgggtccag cggggactcc ccgttgcgca
     2281 ccttccgcac ggcctccacg atctcctcgt gaggcgcatc cttgagtacg tagccggcag
     2341 cccccgcctt cagcgctccg agcaggtagt cagggttctc gtgcatggtc agcatcatga
     2401 cgctaatctc ggggtatctt tgcttgatct cctttgtggc ttctaaccca tccatgacag
     2461 gcatccgcac gtccatgagg atcaaatcca gcctgaaacg cgagcccagc gagagtgctt
     2521 gccttccgtt ggccgcctcg ccaacgatct cgatgtcggg ctcgtcggag agcatgtttt
     2581 ggagactctc cctggtgagc tcatggtcgt cgactatggc caagcgcgtt ggcccacgta
     2641 cgtcctctgg agagatctcg gttcccactt ctctatacac ccccgattaa caactcgccg
     2701 aacactttca cggttgtcgt ccgatctccg cggctgcaag ggacctgcac ggtagcgtcg
     2761 tcatgccgtt cgcatcagag ggatgatcgc cgcaacgaag gtaccggcct ccgggcggct
     2821 ttggatctcg agctcgccac caagcatgcc gacccgctcc ctcatccccg caaaaccgac
     2881 gcgctcgcca ggcctgcccc ccaacgtttg gagtgcggcc gggtcgaacc cacgcccgaa
     2941 gtcccggacc tccagataga cttctgtgtc tcgccgctgc aactcgatct tcaaccactt
     3001 cgtccccgcg tgcttgcgta cgttggtcaa cgcctcctgc gcgacgcgga agagcgcaat
     3061 ctccatctct cttggcaggc gctcctctcc aagatgttcc tcgcagtcga cctggtagcc
     3121 ttcctcattc aggcgttcga cctccaggga gatggcggcc gcgagtccga ggtcgtcgag
     3181 ggccgtcggc ctcaagttcg cgatgacccg gcgcgcatcc gagaccgtcg tccggacctg
     3241 cccgagtatg cgatccagtt ctctcctgcc ttgctccgtc tctggggcgt agcgccgggc
     3301 gaaggcctgc agattctggt gtgcggccac cgcaacctgg gccaaaccat cgtgaacctc
     3361 gtaggccact cggcgtctct ccacttcctg ggctcccaac aaactctttg ccaggttctg
     3421 cagcgcacgc tcgcggtcgt ccagctcctt gtagagccag gcgttctcga gagcgcttgc
     3481 agcctggctg gtcaggctac ttactatgtc gtagtcagcc aggatgagat cgaccccacc
     3541 ctcctccagc gctgcggcga aggcgtcgcg gctctgtact cggaccagct cgcacccgat
     3601 cccgccttct gacagtcgag ctccgacgag ttctgcgtcc aacgagcaat cttcgaggat
     3661 gaggatgcgc agcacaccag atctatcgcg gccttgccct tgtggtccgt gcaagacctt
     3721 caaagtttcc gccccacagc gctggacggg tggtcgttca ctccgaggag tctcgctttg
     3781 gggtcctccg ccgtctgcga ccggtacgcg gccctgagga ggcggtagtc tagcctcccc
     3841 tcgctcgagg cggagagagt agactcgaca tcgttctggt tgccctcgac gagtaagagc
     3901 gtactgctgt aatccctgtc atccaacact attctccgtg aatcgcggaa gcgagaagta
     3961 gaaggtagcc ccccctccta aacaattatc ctatttacac tcgtcccgcg cggcggccga
     4021 cggtacgtct cacgctcgcg agtccgatgc ccgtactttg tacccttccg cgtcgtgcaa
     4081 gagccgaaat acgccgaaga gctttctgaa atattgtaca gcaaacccca caccaatatc
     4141 gcgaataaag aatacgacct cacgctaggc accagtgctc ccgacccgaa tcaccgccgg
     4201 atcgtgggga tccggcggtg tcaagatcgg gtagacagaa gctcactgcc tctcacgcga
     4261 caggagacgg cggcgggcca gggtccgcag aaggcgatgt tctcgatcaa gacagacgaa
     4321 gataggcctt ctggcacgtt cgctcgtggc gcagcgggta taggataggg tagccaatgt
     4381 tgagaaaccg tcgatccgaa aggcacagct ttgaagatca ctactcgggg taagccgccg
     4441 cgctcagaca catatggggg aaccatacga agtacggtga cgaacgcctc catgggagtt
     4501 cacgagctgc ggttccgtcc cgctccgtgg tggtgaagtc accgttgaca caccctcgga
     4561 gaaaacagac ctcttgccga caaaggaaca ggatgacagg cgaccctccg ccgaccgtcc
     4621 cgatgagatc gcggccgaca cacgccgaag ggcttggtgt ccagcgaagt gtagacttta
     4681 ataggacgta ccagctcttg tgcgggtgta gctacaaggt acgctgcgag gtcatgcgcc
     4741 acggcgagcg attgggcgag ttgacgttct tcgacgatga ggaggcgagc gtaacccaag
     4801 gcgagcgagt ccggtactgc cctgggtgcc gcagccgact cagtctcctt tctgtacgat
     4861 ctcgaggctg gaccaagagg caatcccgat gtggtaagta gagaaggtta ttcgagtgct
     4921 tcggcgtttc acagatgtcc gggaacatgt cgagtggaat gggcgtaccg attcatcgaa
     4981 gaggtcgctc gtcagggaaa actacgcccc gccgttctcg acaggggcgt atccaacggc
     5041 ttagggaaga tcctggaagc cggatcctag aagttcgcca catccttctc ggcggtccag
     5101 accgcggggt cttcggcgcc ttccgcctct ccctgctcct cgagccaacg ctcgctgtcg
     5161 atggcggccc tgcagccgtc gccggcggcc gttacagcct ggcggtagcg ggtgtctgag
     5221 acatcaccag cggcgaacac gccggggacg ctcgtcatag tgttttcctt ttgtatgatg
     5281 tagccgccct ctttcatctc gacctgcccc tcgaaaagag aggttgcagg ggtgtgcccg
     5341 atggccgtga agaagccgct cacgtcgatg gttcgctcct cgtctgtctt ggtgttcctt
     5401 gtgcgcaccc cttcgacggc gtctttgccc aggatctcct ccacgaccgt gtctgttatg
     5461 aacgtgatct tggggttctt acgcgccctt tcgagcatga tcctggacgc cctgaactcg
     5521 tcgcggcggt ggatcaagaa aacctcgtca gcgaacttgg cgaggacgcc ggcctcctcc
     5581 atcgctgtgt cgcccccgcc gactacggcg accttcttgc ccctgtagaa gaacgcgtcg
     5641 caggttgcgc agccgctgac acccttgccc atcaactgct gctcgctatc gagcccaagc
     5701 cacttggcct tggcacccgt ggcgatcacc acggcctttg cgagggtagc ctcatcctca
     5761 tcttcggccc acaacttgaa cgggcgctcc gagaaatcca ccctctcgat gttctcggga
     5821 cgcatctcgg ccccgaaacg cgcggcctgg gcctcgaagt cgtccatcat ctcaggcccc
     5881 atgacgccgt ccctgtaccc ggggtagttc tccacgtcgg aggtcagcat cagctgcccg
     5941 ccgctctcga aaccctggaa cacgagcacc ttcaggttcg cccgcgcggc gtacagggcc
     6001 gccgtgtacc ccgcaggacc cgagccgatt atcacgacgt catatgctct gtgagccatc
     6061 taaaacaccc ctcttgaatg caaaatctct ggccccgtta gtttaatata tctagcaagc
     6121 cgtatggtgt tagctttacg tcgcagctac tgctgcaacc tcactcgcgc cagccggcca
     6181 ggaacctctc ctgctccggt gtcagtgcgt cgacgctcat gccaacggcg tcgagcttca
     6241 ttgtggccac ctcatcgtct atctccctgg ggaggaggtg taccttgttc tccatggagg
     6301 cgtgctcgtt cgcgaggcgg taggcgcaca gggcctggat cgcgaaggtg aggtccatga
     6361 tctcgacggg gtgcccgtcg ctcgccgtca ggttgacgac cattccgcca ccgacgaggt
     6421 gtaatctacg gccgtcagag gtggtgaact cctcgacgtt gctgcggacc tcgcgtgcgc
     6481 gcgtggcggt agccgcaagg ccgtccacgt ctatctccac gtcgaccccg ccggcgttgg
     6541 ccaggatcgc accgtcgtgc atccgctcga agtgggctgc cgtcagtacg tcgcggcttc
     6601 ctgtcgccgt aatgaacacc tccccgatct ccgcggcgtc cagggccggc agcacgtcga
     6661 agccgtccgc gtaggcttcc aggccgcgca cggggtcgac ctcgcagacg gagacacggg
     6721 cgccgagccc agcggcgtag cgggcgatac ccttgccgca ccatccgtag cccagcacga
     6781 cgacgcgttt gccgccgacc atcaggttcg tgttctgcat gatcgccgtt agcgtggact
     6841 gccccgtccc gtagcggtta tcgaagaggt acttgcacct ggcgtcgttc gccgcgataa
     6901 ctgggaactc gaggactccc tcctgctcca tggccctgaa cttcgccacg ccgctcgtcg
     6961 tctcctcaga cgcaccgtgg atgtgcccca gcaggtttcg gcgcgtcgtg tgcgcgagac
     7021 gcactagccc ggccctgtcg tctataaaga cggcctccct gtcgcccgcg gcccggtcga
     7081 gcacacgctc taactccctg tcggcgtctt ctgggtcgtt ctcggaggag gcgtagaccg
     7141 tgacgccacg ctcggccaga gcggcggcca cgtcgtcctg gacgaagaac ggtccgggac
     7201 tcgctacggc cacgtctgcc ccggcttcgg cc
//
LOCUS       DASTKM010000206        11425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1293589, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000206 DASTKM010000000
VERSION     DASTKM010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11425
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293589"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFQ10_08520"
     CDS             <1..250
                     /locus_tag="VFQ10_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9965426.1"
                     /translation="PIAPAVIVGTDITHAMVLSFVVGIAHMTQGNVDFALAGTLLLGS
                     IPGVLIGSRLAPWIPGKPLKLLLAIMLIFVGARLLLAG"
     gene            complement(281..1048)
                     /locus_tag="VFQ10_08525"
     CDS             complement(281..1048)
                     /locus_tag="VFQ10_08525"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9965427.1"
                     /translation="MQLENKVAAVTGSSLGIGRAIALAFGREGASVAVNYRSHPEEGA
                     RAVQEIERGGGKAISVQADVSEPEGVRNLIRQTVKEFGRLDVMVNNAGIEQKIPFLET
                     PLEVWEKVLSVNLTGVWLGCQEAASQMVSQGDGGRIINVSSVHEDLPMPTNSPYCAAK
                     GGVRMLMRTIAVELAPHGITVNNIAPGAIETPINRNLDEHPEQRQELLSQIPLGRIGQ
                     PEDVASMAVYLASDASSYSTGSTFFVDGGLMRHSASL"
     gene            complement(1032..1829)
                     /locus_tag="VFQ10_08530"
     CDS             complement(1032..1829)
                     /locus_tag="VFQ10_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET9965428.1"
                     /translation="MRIGNDAAGQVQGDVPGEVLAAAHVGRTAGIVPMERGWQLQRWL
                     AGRLQGAWMRPDNGIWESRGERQHFVYSKVMCWVAFDSLVKGVERFGLDGPVERWRAA
                     RDEIHSDVLEKGYDAARGTFTQAYGSRALDACALLIPRVGFLPYDERVISTVETIRNE
                     LTEDGLVLRYRTDQSDDGLAGREGSFLICSFWMVYALLGIGRHREAEDLFGRLLALRN
                     DVGLLSEEYDTRAQRLVGNFPQAFTHLALVNCALRLSEGEVGSAARE"
     gene            complement(1820..2815)
                     /locus_tag="VFQ10_08535"
     CDS             complement(1820..2815)
                     /locus_tag="VFQ10_08535"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET9965429.1"
                     /translation="MPGRIEDYAMVGDLQTVALVGSDGSVDWLCFPRFDSPACFAALL
                     GNDSNGRWRLAPTSGGRCTRRRYRGHTLVLETEWETPTGAVRVIDCMPPRGVAPDLVR
                     VVEGVRGKVLLTSELRIRFDYGHVAPWVTIEGREVHAVAGPEALWLRASPPHEEVGGE
                     ILSRFTLSEGERVSFVLTWSHSYGERPVAIDGLQAVDDTERYWLDWAGGIGYDGRFHQ
                     DAVYRSLLTLKGLTYEPSGGIVASATTSLPETLGGSRNWDYRYCWLRDSTFTLGALVG
                     AGLHEEARAWQQWVLRAVAGDPADLQIMYGIDGTRRLPEYELPWLPATKAPRRCE"
     gene            2935..3072
                     /locus_tag="VFQ10_08540"
     CDS             2935..3072
                     /locus_tag="VFQ10_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965430.1"
                     /translation="MSEHTLRRRFWFWGRLPGVTHEQALANMSLFASRVAPRVRDAVK
                     V"
     gene            3069..4133
                     /locus_tag="VFQ10_08545"
     CDS             3069..4133
                     /locus_tag="VFQ10_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HET9965431.1"
                     /translation="MRTLTSVLGEDQAAFLVRQRVARLATSDGMGKPHAVPVCFALGR
                     ASIFIALDEKPKDVPVTRLKRVRNILENPHVALISDRYTEEWNLLAFVMVRGRAELLD
                     PDTEEHAAAVRLLRGKYHQYETMRIDENPVIAIRSEKAASWGALDEPDTSGPQVLDTI
                     HGRRSVRRYLQKRVPEDLVERVLEAARWSPSPHGRQPWRFAVLTKEATKEHLARAMGE
                     EWRANLEMDGQSVEIVEKRLEGSRRRLLDAPVLVLICLYLEDLDAYPDAERQRGETTM
                     AVQSLGAAAQNALLTAYDSGLDAGWMCAPLFCPEKVVEALGLDPKLVPHALLTLGYAE
                     GDPPKRRSRRPLDELVVYRD"
     gene            4356..5225
                     /locus_tag="VFQ10_08550"
     CDS             4356..5225
                     /locus_tag="VFQ10_08550"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET9965432.1"
                     /translation="MTEQEIQEKGYANPDALVSTQWVAEHLDDTENVRIVESDEDVLL
                     YDIGHVPNSVKIDWVEDLNDPLMRDYLDPDHFANLMAELGISPDTTIIFYGDKNNWWA
                     TYALWVFGLFGHDNVKVMDGGRVKWEAEGREMTQEVPSFPRVDYPVPQRDDSRIRAFR
                     EDVERHLEARGKMIDVRSPGEYRGELLHMPDYPQEGALRGGHIPGAANVPWARAVRED
                     GTFKSSDELKEIYEGEVGLSQGDDVIAYCRIGERSSHTWFVLNYLLGYEDVRNYDGSW
                     TEWGNLVRAPIER"
     gene            5230..5682
                     /locus_tag="VFQ10_08555"
     CDS             5230..5682
                     /locus_tag="VFQ10_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET9965433.1"
                     /translation="METKSKEPDRKKNGAALLMEHASNPRHKNEESVQEDADVAMPGG
                     SPECGGSVVVYLKGGDDGKIERLSWTGQGDTISMGATSIVVERILGEGLSMEEVLDLD
                     YEEFIDSLGRELIGSRTRHATLGLSTIKGAVRIYQRKSRSEDKERAGG"
     gene            5772..7217
                     /gene="argH"
                     /locus_tag="VFQ10_08560"
     CDS             5772..7217
                     /gene="argH"
                     /locus_tag="VFQ10_08560"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET9965434.1"
                     /translation="MSDGGKNTSGRMWGGRFGAPPAEAFERLNASIPFDIRLAPHDIR
                     GSVAHAKMLGAQGVISDRESATLVRGLEAVLREVESGEFAWDLSDEDVHTAVERRLKE
                     LVGDVALKLHTGRSRNDQAALDLHLFAREKAERIRSGVLAAMHALVGLAETNKGLVLP
                     GYTHLQRAQPILLSHHLLSHFEAFRRDLRRFEAAAEAANVSPLGAAALGGTPHPVDPR
                     LTAQELGMEPFTNSLDAVSERDFVLDLLYACAVLGVHLSRLGEEWVLWTSAEFGFATL
                     GDAYSSGSSIMPQKKNPDAYELMRGKTGRLIGDLNGVLITLKGLPLGYSKDLQEDKEP
                     LFDAVDSVLAMLAVLPEMLRTARFDGGRMAEAAGGFALATELADFLAAKGIPFREAHD
                     AVGRLVRRCEELGVPLENVPQEELVAAHPALWEVPDGLLTPRGSVQNKVSAGSTAPGS
                     VDEQLKQAKAFLKTMGSLSEGQVSEGSDRCE"
     gene            7208..8089
                     /locus_tag="VFQ10_08565"
     CDS             7208..8089
                     /locus_tag="VFQ10_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9965435.1"
                     /translation="MRIVQMSDVHVGSGLFRQDLLEATIEESNALEPDLVAIVGDLTM
                     EGYRWEFEEAKEYFDRLECPNVIYAMGNHDAKNVGYRHFEEFFGIRERSAQIPVPEGE
                     AKVVALDSTKPDLDEGEVGREHYSWLDSEFRDWESGPRILMIHHHILAVPGTGRDVNN
                     LRDAGDVMAILRELKIDMVLSGHRHVPYVWSISGVRIVHSGTVSTMRVRGTMPPSYNV
                     VEIDEQSVNITLHQPGKGEEGKEPLASFSRTPVTTSEFYPAFERFVLYDRLPFLTTGI
                     DKSSSDSASGAGDGPKR"
     gene            complement(8002..9636)
                     /locus_tag="VFQ10_08570"
     CDS             complement(8002..9636)
                     /locus_tag="VFQ10_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET9965436.1"
                     /translation="MPGETSNGHGTDSSERVALYMRVSSEEQRDRETIEIQRGFLQEY
                     CRLYGLEVAQTYADDGVSGTIPLHERPEGRRLLEDAKERKFSTLLVYRLDRLGRSLLV
                     TVEAHDRLQALGVALKSATEPIDTSNPSGRLIFQMLASFAEYERETIRERTRAGLHRA
                     YRGGKHFGAAPYGYRTDDHGLLQVVPEEAEIIREIIESVAEGSTLYAEAKRLNDLGLA
                     TPGWRYGNGKKRPGAKLWSVMTVSNIVRQSAYSGVHRVKTNGGKDVIEQSVPAVLEPG
                     LQERAMAVLEENSRYRNRKNDRRYLLRSLVRCSTCGGACTGHTSSRRGKKYHYYACRA
                     AKTYNFGMAPPHKASFVSAEWLEELVWSDVRRFLEAPGEVLERLREQHDAADDAGELE
                     DRRKELAKRLAAKQAEKDRYVRAYAQGHISEEELEVYLAELKKQTDNVRVLLASIEAE
                     LSERRERTALTDTTHAWLLSLSQCLDEVEEDTPEGFEKRKQLVGLLVESISLGKSQQE
                     GRAEVQITYRFGPSPAPDAESEEDLSMPVVKNGSRS"
     gene            complement(9639..9827)
                     /locus_tag="VFQ10_08575"
     CDS             complement(9639..9827)
                     /locus_tag="VFQ10_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9965437.1"
                     /translation="MNRLQELRINRGLSQRQLAELAGIANTSLWKIERGGGAHPATLK
                     KLANVLGVLPTDLLKKGR"
     gene            complement(10384..10611)
                     /locus_tag="VFQ10_08580"
     CDS             complement(10384..10611)
                     /locus_tag="VFQ10_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965438.1"
                     /translation="MSDGCTNELLQANPGGYLCWQEEQRGKAELKRKQAEERDDSEQF
                     ARMLIERGRDPSNSREMYERCRNHMALEEVI"
     gene            complement(10692..>11425)
                     /locus_tag="VFQ10_08585"
     CDS             complement(10692..>11425)
                     /locus_tag="VFQ10_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965439.1"
                     /translation="YYGPSGAFSQEVINELGDPHEERGWRLPLEISPTAIAELMVENN
                     RWGFEGWPVLAVREIRRRRNIGYPRIVREVAKLLRATPFRNKCPVMGIAADRTGVGVH
                     AIQSLAEHGVYTDAAIFIHGGQNDKRDSAGVNFFNVAQRNLIGYAQGLAEETERDRLK
                     FNPLLPLAEVARRELTNFRVNQDPKTNHESFGAPKREGEFDDIVYSVAMACWLRHYHV
                     STEAQWMGEVVGPDLRGPNPWAGLY"
BASE COUNT         2122 a         3571 c         3596 g         2136 t
ORIGIN      
        1 tcccatagca ccggccgtta tagtcggcac tgacattaca cacgcaatgg tcctctcgtt
       61 cgtcgttggc atagcccaca tgacgcaggg caacgtggac ttcgccctgg ccgggaccct
      121 gctgttgggc agcatccccg gtgtactgat cggaagccgg cttgcgccct ggatcccagg
      181 caaaccgctc aagctgctgc tggccatcat gctgatcttc gtaggtgcgc gcctgctcct
      241 tgcaggctaa atggcttctt ctggacgacg gggttcacgt ctacaggctc gctgagtggc
      301 gcatcaggcc gccgtccacg aagaatgtgc ttcctgtgga gtaggaagag gcgtccgaag
      361 ccaggtagac ggccatggag gcgacatcct cgggctgccc gatcctgcca aggggaatct
      421 gggagagcaa ctcctgcctc tgctcgggat gctcatcgag gttccggttg ataggggtct
      481 cgatggcgcc tggggcgatg ttgttcacgg tgatgccgtg cggggccagc tcgacggcga
      541 tagtccgcat cagcatcctg acgccccctt tggccgcgca gtagggagag ttggtcggca
      601 taggcaggtc ctcgtgcacc gaggagacgt tgatgatgcg ccccccatcg ccctgtgaga
      661 ccatctggct ggcggcttcc tggcagccga gccacacccc tgtaaggttt accgagagca
      721 ccttctccca cacctcgagg ggcgtctcga gaaagggaat cttctgttct atgcccgcgt
      781 tattgaccat gacgtcgagg cggccgaact ccttgacggt ctgccggatc aggttcctca
      841 caccctcagg ctctgagacg tcggcctgta cagagatggc cttgcctccg cccctctcta
      901 tctcttggac cgctcttgcc ccttcttcgg gatggctcct gtagttcacg gccactgagg
      961 ctccttcccg cccgaaagcg agcgcgatgg ccctgccgat cccgagggaa gagcccgtta
     1021 ctgcggcgac cttattctct agctgcactg ccaacctccc cttcggatag tcgtagcgcg
     1081 cagttgacca acgccaggtg tgtaaacgcc tgtgggaagt tgcccaccag cctctgtgcg
     1141 cgcgtgtcgt actcttcgct gagcagccca acatcgttac gcagagcgag aagccgaccg
     1201 aataggtcct cggcttcccg gtggcggcct atgcccaaca gcgcgtagac catccagaag
     1261 ctgcagatca agaacgagcc ctcgcgtcct gcgagaccgt cgtcggattg gtctgttcgg
     1321 tagcgaagta cgaggccgtc ttcggtcagc tcgttgcgga tcgtctcgac ggtactgatc
     1381 acccgctcgt cgtagggcag aaaccccacg cgtgggatga gtagcgcgca ggcgtcgagt
     1441 gccctggagc cgtaggcctg ggtgaaggtc ccccgtgcgg cgtcgtagcc tttctccagg
     1501 acgtcggagt ggatctcgtc ccgcgcggcc cgccagcgct ccacgggacc atcgagcccg
     1561 aaacgctcga cgcccttgac cagagagtca aacgcaaccc agcacatcac cttggagtag
     1621 acgaagtgct gcctctcccc gcgcgactcc cagatcccgt tgtcaggtct catccaggcc
     1681 ccttgcaggc gccctgcgag ccagcgctgc agttgccaac ccctctccat agggacgatc
     1741 cccgcggtcc gacccacgtg cgcggcggcg agcacctcgc ctggtacgtc gccctggact
     1801 tggcctgcgg cgtcgttgcc tattcgcacc ggcgaggcgc cttcgtagcc gggagccagg
     1861 gcagttcgta ctcgggcagg cggcgggtgc cgtcgatgcc gtacatgatc tggaggtcgg
     1921 cggggtcgcc ggcgacggcc cttagcaccc actgctgcca ggcgcgggcc tcctcatgca
     1981 ggcctgctcc taccagcgcg cccagggtga aggtgctatc gcgcagccag cagtaacggt
     2041 agtcccagtt cctggagccg ccgagggtct cggggagcga ggtcgtggcg gaggcgacga
     2101 tgccgccgct cggctcgtag gtcagcccct tgagggtcag gagggagcgg tacacggcat
     2161 cttggtggaa gcggccatcg tacccgatgc cgccggccca gtcgagccag tagcgttccg
     2221 tgtcgtccac cgcctgcaga ccgtcgattg ccacaggacg ctcaccgtaa gaatgcgacc
     2281 aggtgagtac gaaagagacg cgctctccct cggagagcgt gaaccgggag aggatctcac
     2341 cgcccacctc ctcgtggggg ggcgaggcgc gcaaccacag cgcctctgga cccgctacgg
     2401 cgtgcacctc acgtccttct atggtgaccc acggcgcgac gtgtccgtag tcgaagcgta
     2461 tccttagctc gctcgtgagc aatactttgc cccttacgcc ctcgaccacc cgaaccaggt
     2521 cgggcgccac accgcggggc ggcatacaat cgatcaccct cacggcgccg gtcggggtct
     2581 cccactcggt ttccaggacg agcgtgtgcc ccctgtaacg tcgcctcgtg cagcggccgc
     2641 ctgaggtggg ggccagacgc cagcggccgt tagagtcgtt gccgagcagc gcggcgaagc
     2701 aggccggcga atcgaagcgg gggaagcaca gccagtccac ggaaccgtca gagccgacca
     2761 gggccacggt ctgcagatct cccaccatcg cgtagtcttc gatcctgcct ggcatcagac
     2821 ccctcctgca aaaccacggc caataaacta tgccagacgg tggggtactc ggccaccact
     2881 gctgcggcgg cgtggggaag acccgaggac ttccctatct tgtagactaa gtgcatgagc
     2941 gagcacactc ttaggagacg cttttggttc tggggacgtc tccccggtgt cacccacgaa
     3001 caggcgctgg ccaatatgag cctgttcgcc tccagagtcg ccccgcgcgt ccgcgacgca
     3061 gtcaaggttt gaggacactc acgagcgtac tgggtgagga tcaggccgcc ttcctcgtac
     3121 gccagagggt ggcccgtctc gctacctcag acgggatggg caaaccgcac gccgtacccg
     3181 tgtgcttcgc cttggggcgt gcttcgatct tcatagccct cgacgagaag ccgaaagacg
     3241 tacccgtaac ccgtctcaag cgcgtcagga acatcctcga gaacccccat gtagcgctca
     3301 tatccgaccg ctacaccgag gagtggaacc tcctggcttt cgtgatggtg cgcggcaggg
     3361 ccgagcttct ggaccctgac accgaggagc acgccgcagc cgtgaggctc ctgagaggta
     3421 agtaccacca gtacgagacg atgcgcatag acgaaaatcc cgtcatcgcc atacgctccg
     3481 agaaagctgc ctcgtggggc gccctcgacg agcccgacac ctccgggcct caggtcctcg
     3541 acacgattca tggccgccgc tccgtcaggc gttatctcca gaagagggtg ccagaggatc
     3601 tcgtggagcg ggtactcgaa gccgccaggt ggtccccttc cccccacgga cgacaaccct
     3661 ggcgatttgc cgtgctcacc aaagaagcga ccaaagagca tctggctcgc gcgatgggcg
     3721 aggagtggcg ggccaacctg gagatggacg ggcagagcgt ggagatcgtc gagaagcgtc
     3781 tcgaaggctc ccgcagacgc ctgcttgacg cgccggtact cgtcttgatc tgcctttacc
     3841 ttgaagatct cgatgcttat cccgatgcgg agcgccagcg cggtgagaca acgatggccg
     3901 tacagtcttt aggggctgcc gcccagaatg cgctgctgac cgcctacgac tcaggcctcg
     3961 acgcaggctg gatgtgcgca cccctcttct gccctgagaa ggttgtcgag gcgctgggcc
     4021 tggacccgaa gctggtgccc cacgcgctgc tcacgctcgg ttacgctgag ggcgacccgc
     4081 caaagcgccg ctcgcgccga cctctcgacg agctcgttgt ctacagggac tgagcgccct
     4141 acgtggtcat aaacgacgtg ccgatagatt tggcgtccgt acccggaagc cgtgcccccg
     4201 tgtgctttcg cggaggggca gtataccctt ggatccctcc gatagaatag gcctccacgt
     4261 gcccggtccg ttcccgaact caaactcgat aaactctatc ggagataagt tagaatgtgc
     4321 gctcgtgcag tagtcaagat gactgaggag gctctatgac ggagcaggag atacaggaga
     4381 aaggttacgc gaaccctgac gccctagtaa gtacccagtg ggtcgctgag cacctggacg
     4441 acacggagaa cgtacgcata gtcgaatcgg atgaggacgt gctcctctac gacatcggcc
     4501 acgtgcctaa ctctgtgaag atagactggg tcgaggacct caacgatccg cttatgcgtg
     4561 attacctgga cccggatcac ttcgccaacc tcatggcaga gctgggcatc tcccctgaca
     4621 cgacgatcat cttctacggt gacaagaaca actggtgggc gacctacgcc ctctgggtgt
     4681 tcggactctt cggccacgac aacgtgaagg ttatggacgg tggcagggtg aaatgggagg
     4741 ccgaggggcg cgagatgacc caggaggtgc cctcgtttcc cagggttgac tatcccgttc
     4801 ctcagaggga cgactccagg ataagggcgt tcagggagga cgtggagagg cacctggagg
     4861 ccaggggaaa gatgattgac gtgaggagcc ccggtgagta caggggcgag ctactccaca
     4921 tgcctgatta cccacaggaa ggcgctttga ggggagggca catcccaggg gccgctaacg
     4981 tgccgtgggc gagggctgtc agggaggatg ggacgttcaa gagctccgat gagctgaagg
     5041 agatctacga gggagaggtc ggcctcagcc agggggacga cgtaatagcc tattgcagga
     5101 taggagagcg ctcttcccac acgtggttcg ttttgaatta ccttctcggt tatgaggacg
     5161 tgcgtaacta cgatggctcc tggaccgagt ggggtaacct agtgcgggct cccatagaga
     5221 gatagaggtt tggagacgaa gagtaaggag ccggatcgca aaaagaacgg cgccgcgctc
     5281 ctgatggagc acgccagtaa tcctcgccac aagaacgagg aaagcgtaca agaagacgcc
     5341 gatgttgcca tgccaggcgg cagccccgag tgcggggggt cggtggttgt ctacctcaag
     5401 ggaggcgacg acggcaagat agagcggctc tcttggactg gtcagggtga caccataagc
     5461 atgggggcaa cctccatagt ggtcgagcgc atcctgggcg agggcctcag catggaagag
     5521 gtcctggatt tggactacga agagttcatc gactccttgg gtagagagct cataggtagc
     5581 cgcacgaggc atgccacgct cgggctctct accataaagg gcgctgtaag gatctaccag
     5641 aggaagagcc ggtctgaaga caaggagcga gccggaggct aggctccgag tccgcaggcg
     5701 ccgccgtcga agcctgccgg cacagacgtg atccccgtct ccatctacaa gagccgggag
     5761 ggagaagctt catgagcgat ggaggcaaga acacgtctgg gcggatgtgg ggaggaaggt
     5821 tcggagcccc acctgcagag gccttcgagc gcctcaacgc ctccatcccc ttcgacatcc
     5881 gtctcgctcc tcacgacata cgtggctccg tcgcccacgc gaagatgctg ggggcgcaag
     5941 gggtaatctc ggatagagag tcagcgacgc tggtacgtgg gctcgaagcg gtgctacggg
     6001 aggtagagtc gggggagttc gcgtgggatc tctcggacga ggacgtgcac acggcggtag
     6061 agcgcaggct gaaggagctc gtgggcgacg ttgctctaaa gctgcacacg ggccgcagcc
     6121 gcaacgatca ggccgccctc gatctgcacc tctttgccag ggagaaggca gaacggataa
     6181 ggtcaggcgt gcttgccgcg atgcacgcgc tcgtcgggtt ggccgagaca aacaagggcc
     6241 tcgttctacc cggatacacc catcttcagc gagctcagcc gatcctgctc tcccaccacc
     6301 tgctctccca cttcgaggcg tttcgccggg acctccgaag gttcgaggca gccgcggagg
     6361 cggcgaacgt ctcgccgttg ggcgcggcgg ccctgggcgg tacgccgcat cccgtagatc
     6421 ccaggctcac ggcccaggag ctggggatgg agccgttcac gaactcgctg gacgcggtct
     6481 ccgagcggga cttcgtcctc gacctcctct acgcctgcgc cgtactcggg gtgcacctga
     6541 gcaggctagg ggaggaatgg gtgctgtgga ccagcgccga gttcgggttc gccaccttag
     6601 gcgatgcata ctcttcgggc tcctccatca tgccccagaa gaagaacccc gatgcctacg
     6661 aactcatgcg cggcaagaca ggccgcctca tcggggactt gaatggggtc ctgatcaccc
     6721 tcaaaggcct cccgctcggc tactcgaaag acctccagga ggacaaagaa cctctctttg
     6781 acgcggtcga ctctgtgctc gccatgctcg ccgtcctgcc cgagatgttg cggacagcgc
     6841 gcttcgacgg aggacggatg gccgaggcgg cagggggttt cgccctcgcc acggagctgg
     6901 cggacttcct cgcagcgaag ggaatacctt tcagagaagc ccatgacgcc gtagggaggc
     6961 tcgtgcggag gtgcgaggaa ctgggagtgc cgctagagaa tgtgccgcaa gaagagctcg
     7021 tggcggcgca tcccgcgcta tgggaggtgc ctgatgggct gcttacgccg cgcggaagcg
     7081 tgcagaacaa ggtctctgcc ggctccacgg cccccggttc cgtcgatgag cagctaaagc
     7141 aggcgaaggc attcctaaag acaatgggaa gtctatcgga gggccaggtt tcagaaggga
     7201 gtgacagatg cgaatagtac agatgtccga tgtccacgtg ggttcgggac tgtttcggca
     7261 ggatctcctc gaggccacca tcgaggagag caacgctctg gagcctgacc tcgtggccat
     7321 cgtgggggat cttacgatgg agggatatcg ctgggagttc gaggaggcga aagagtattt
     7381 cgaccgtctg gagtgtccga atgtaatcta cgcgatgggc aaccacgatg ccaagaatgt
     7441 cggctacagg cacttcgagg agttcttcgg cataagggag aggtcggccc agattcccgt
     7501 ccccgaggga gaggccaagg tcgtcgcgct cgactcgacc aagccggatc tcgacgaggg
     7561 tgaggtcggg cgcgagcact acagctggct cgactccgag ttccgcgact gggaaagtgg
     7621 gccgaggata ctcatgatcc accaccacat cctcgccgtc ccaggcacgg ggcgcgacgt
     7681 caacaacctg cgtgacgccg gcgacgtgat ggccatcctg cgcgagctga agatcgacat
     7741 ggtcctctcg ggccacaggc acgtccctta cgtctggagc atctcggggg tgaggatcgt
     7801 tcactcagga accgtatcga ccatgcgcgt gcgcggcacg atgccgcctt cttataacgt
     7861 tgtcgagatc gacgaacaga gcgtcaacat tactctgcac cagccaggca agggtgagga
     7921 gggcaaggaa cccctcgcct cattctcccg cacccccgtt acgacaagcg agttctaccc
     7981 tgccttcgag cgcttcgttc tctacgatcg gctaccgttc ttgacaacag gcatagacaa
     8041 gtcctcctcg gactcggcgt caggggccgg ggacggtccg aaacggtagg ttatctggac
     8101 ctcggcccgg ccttcctgct ggctcttgcc gagcgagatc gactccacga gcaacccgac
     8161 gagctgcttg cgcttctcga acccctcggg cgtgtcctcc tcgacctcgt ccaagcactg
     8221 actgagcgag aggagccagg cgtgcgtggt gtccgtcaac gccgtccgct cccgtctctc
     8281 cgagagttct gcctcgatcg aggccagcaa gacccgcacg ttgtcggtct gcttcttcag
     8341 ttcggccaga taaacttcta gctcctcctc cgagatgtgt ccctgggcgt aggccctgac
     8401 gtaccggtcc ttttcggcct gcttggcggc gagacgcttg gcgagctcct tcctgcggtc
     8461 ttcgagctct ccggcgtcgt ctgctgcgtc gtgctgctcg cgcaggcgct caaggacctc
     8521 tccaggggcc tccaggaaac ggcgcacgtc cgaccacacc agctcctcaa gccattcggc
     8581 gctcacgaag gaggccttat gcggtggcgc catgccgaag ttgtaggtct ttgcggcacg
     8641 gcatgcgtag tagtgatatt tcttgcctct cctactcgag gtgtggcccg tgcaggcgcc
     8701 gccgcaggtg gagcaccgga cgagactcct cagcaggtac ctgcgatcgt tcttccggtt
     8761 gcggtacctc gagttctcct cgagaacggc catcgcccgc tcctggagac ccggctcgag
     8821 tacggccggc accgattgct cgatgacgtc cttgccgccg ttcgtcttga ccctgtggac
     8881 gcccgagtag gcgctctggc gaacgatgtt ggagaccgtc atgacagacc agagcttggc
     8941 gcccgggcgc ttcttgccgt tgccgtagcg ccatccaggg gtggcgaggc cgagatcgtt
     9001 gaggcgcttg gcctccgcgt agagcgtgga gccttcggcg acgctctcga tgatctcgcg
     9061 tatgatctcc gcctcttcgg gcaccacctg gagcaatccg tggtcgtcgg tgcggtagcc
     9121 gtagggcgcc gctccgaagt gtttgccgcc cctgtacgct cggtgcaggc ctgcccgcgt
     9181 cctctctcgg atggtctcgc gctcgtactc cgcgaacgac gcaagcatct ggaagatgag
     9241 cctgccgctg gggttggagg tgtctatggg ctcggtggcg ctcttcaggg ccacgcccaa
     9301 ggcctgtagc ctgtcgtggg cctcgacggt taccaggagg gacctaccga gccgatccag
     9361 acggtagacg aggagcgtgg agaacttgcg ctctttggcg tcctcgagga gccgcctacc
     9421 ttctggcctc tcatgcagag ggatggtgcc agatacaccg tcatcagcgt aggtttgtgc
     9481 cacttccaga ccgtagaggc ggcagtattc ctgcaggaac ccgcgctgga tctcgatggt
     9541 ctcgcggtcg cgttgctcct cggaggagac gcgcatgtac agggcgacgc gctccgagga
     9601 gtcagtgccg tggccgttcg atgtctcgcc gggcacgact atcgcccctt cttgaggagg
     9661 tccgtgggca gcacccccag gacgttggcc agcttcttga gggtggccgg gtgcgccccg
     9721 ccgccccgct cgatcttcca caagctggtg ttggcgatcc cggccagttc ggccagctgc
     9781 cgctgactga gaccccggtt tattcgcagt tcctgcagcc tgttcacgtc cacctccata
     9841 tgggggatta tatgcgaata ttataccaca aaaggtaaca ttagactacg aacgtggtag
     9901 ggtaggctct atgtgcctgg gtatgaatcc gttctatgaa tccgttcaga gcggcgaagg
     9961 aggagtgttg ttagcataga tggcgtggca ggaattaccc ctcaagagtt cgtcaccaag
    10021 tagagcaccg tcgagcaata ggaaaagtgg ctgcgcaatc ccactttatc gatctgtgcc
    10081 gcttgctgga tcagccgggc cccacggagg cggacccgac cggggatcgg tacacgctca
    10141 acaaaggcgc gaccaaggcg cctggaccga ccaccagtaa gcgcggttgg gccgatttgt
    10201 ggaagaaggg ctgctttgct tgggagtaca aggggccaca cgccaaccta gactaggctt
    10261 atcagcagct ccagcaatac cgggagtccc tggagaaccc ccgctgctcg tggtgtcgga
    10321 catacagacg atcaaggttc ataccacctt caacaaccgt tcgaggcggt aacctccttc
    10381 atcctagatg acctcctcga gggccatgtg gttgcggcac cgctcgtaca tctcacggga
    10441 gttggaaggg tcccttcccc gctctatgag catccgagca aactgctcgg aatcgtccct
    10501 ctcctccgct tgcttgcgct tgagctcggc cttaccgcgc tgctcttctt gccagcacag
    10561 gtagcccccg gggttggcct gcagcagctc gttggtgcat ccgtcgctca ttacttatct
    10621 actcgtagcg tcttgatttt gactcatgtt tccagccact gtgtcctatt cacccttgcg
    10681 cgacgccctt cttagtagag cccggcccag ggattcgggc ccctcaagtc tggcccgacg
    10741 acttccccca tccactgcgc ctcggttgag acgtgatagt gcctgagcca gcacgccatc
    10801 gccaccgagt agacgatgtc atcgaactcc ccctctcgct tcggggcacc gaacgattcg
    10861 tggttggtct tggggtcctg gttcacccgg aagttcgtaa gctccctgcg cgccacctca
    10921 gccagcggca ggagggggtt gaacttcagc cggtcgcgct cggtctcctc ggcgaggccc
    10981 tgagcgtagc cgatgaggtt gcgctgggct acgttgaaga agttcacccc ggctgaatca
    11041 cgcttgtcgt tttgcccacc atggatgaag atggccgcat cggtgtacac gccgtgctcg
    11101 gcgagggact ggatggcgtg gacgccaacc cccgtgcggt ccgccgctat gcccatcacg
    11161 gggcacttgt tcctgaaagg cgtagcccta agcagcttgg ccacctctcg gacgatgcgc
    11221 gggtagccta tgttcctgcg tcggcggatc tccctgaccg ccaggacggg ccagccctca
    11281 aaaccccaac ggttgttctc gaccatgagc tcggctatgg ccgtaggcga gatctccagc
    11341 ggcaaccgcc acccgcgctc ctcgtgcggg tcgcccagct cgtttatcac ctcctgggag
    11401 aacgcccccg aagggccgta gtaga
//
LOCUS       DASTKM010000207        14130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1117271, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000207 DASTKM010000000
VERSION     DASTKM010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117271"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFQ10_08590"
     CDS             <1..199
                     /locus_tag="VFQ10_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965440.1"
                     /translation="RLTADGKIHPCLLTDLDIPVKDAIRSEDPIPAIHEVLWETARIK
                     PAAGLTLPEESRHRTMSQIGG"
     gene            complement(196..918)
                     /locus_tag="VFQ10_08595"
     CDS             complement(196..918)
                     /locus_tag="VFQ10_08595"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HET9965441.1"
                     /translation="MTPLQIIRRAQAGTLIGEDGNVVTLELFPGLSRTELQEFANRLP
                     CRIPPEIEELLGACRGFYGTLEQVDFTGRDLMFEFEQAFPDGLPIAADGYGNFWVVDL
                     HPGTTRWGPIYFACHDAPVILYQADSLEQFLSELFRMFEPPHQSLIDDVHEDRLAHVW
                     RTNPGVLSYEHCLRSEDPILSAFARELGDSFQIIDLRLAKPGDGFSWGRYGPRTQIRR
                     FGTHAVFAYEKPKSVISRLFAL"
     gene            complement(999..1328)
                     /locus_tag="VFQ10_08600"
     CDS             complement(999..1328)
                     /locus_tag="VFQ10_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HET9965442.1"
                     /translation="MRSIPTLHSVADPSASSTETKPGIEIERRVTQEPPYKVILHNDD
                     YTPMEHVVAVLQKVIPRMSVKRAVDIMMEAHTKGKAVATKCHKELAELYQDGLNNEGL
                     ISTIEPD"
     gene            complement(1405..2580)
                     /locus_tag="VFQ10_08605"
     CDS             complement(1405..2580)
                     /locus_tag="VFQ10_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HET9965443.1"
                     /translation="MAAQALSGQAAIVHDLALENYGFGGDHPFDPLRVRLALELCEAL
                     GLLEDYPFVSSGPATDDDLTTVHSLTYVRMVQKAGRSLGPLSDLLDYGLGTPDNPIFP
                     DMHEACARVVGGVLAACRLVMAGEADHAMCISGGLHHAMRSKASGFCVYNDAGVAISR
                     LKEEYPGIRIAYVDTDAHHGDGVQWMFYGDPDVLTVSMHESGRYLFPGSGGVDEKGRN
                     GGVGFSVNVPLEPFTDDESWISCFEAVVPMALGAFDPDLILSQNGCDGHALDPLTHLF
                     ATTRFYEHVPHRIHDLAHQLCGGRWVATGGGGYDIWRVVPRAWTSLWAAVSHQELPEK
                     VSEDWLGKWRVKSPVGLPPLMRDDPEDYPQRPSTAKIAERNLRTVDEVGERVLPRIQ"
     gene            complement(2562..3212)
                     /locus_tag="VFQ10_08610"
     CDS             complement(2562..3212)
                     /locus_tag="VFQ10_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HET9965444.1"
                     /translation="MLLVKDSMTREIVTLAPDETAGTALALCRERRIRHLPVLKEGRL
                     VGIVSDRDLRSSTPALGDPARATALQEILVEDVMTSDVVSVLPDDPIEQAANTMRDRR
                     IGCLPVVEDDELVGILTTSDVMDALVYLVGANEPGSRMEIALPDRPGSLAGAAGVFGM
                     CGINIVSAAMGPTREPADAGEPRERIVVFRVDTIDTAEAVGYLEEAGYSVLWPPRP"
     gene            3289..3663
                     /locus_tag="VFQ10_08615"
     CDS             3289..3663
                     /locus_tag="VFQ10_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965445.1"
                     /translation="MPEYLNQLLADSAMALVRESFTGVSAPWWWERRLGGGIEVCQEF
                     DPETASREISAKTGGEVSEVRRVIAEELGLDDPEPVVLTFEISGETESRHAAELLAER
                     SSEPEGLASGLYRRIEEIVRAG"
     gene            complement(3895..5307)
                     /locus_tag="VFQ10_08620"
     CDS             complement(3895..5307)
                     /locus_tag="VFQ10_08620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9965446.1"
                     /translation="MAGATEFSPMGALDIAGLRDGFRGQLLSPEDPGYDDARRVWNGS
                     IDRFPALIARCAGVADVIAAVKFAKDNGLLVAVRGGGHSFPGYSVCDGGLVIDLSLMK
                     GIRVDPEACTVRAQAGVLLGELDRETQAFGLAVPSGIVTHTGLSGLALGGGINWLMRK
                     YGLTIDQLLSVDLVTAEGELVKASASENADLFWGLRGGGGNFGIVTDFEFRLNPVGPI
                     VLAGPIFWPIEESPKVLRFYREWIAEAPDELMTIVAHRKAPPLPFVPRELHGKLVVGV
                     VCCYSGTVEEGEKVMKPLKVFASPVLDLCVPKPFLTHQAMFDPSFPHGWWYYLRACDV
                     AQLTDEVIDITVDHALRIKSPLSTFVIWQLGGAVAGVGEDETAFGGRSAGHTFNIAGI
                     TESAEGFDEEREWVRNFWSALEPHHTSVYVNFLMEEGEERIRQAYGAKKYDRLKSLKR
                     RYDPDNLFRLNQNINPDVTV"
     gene            complement(5465..5806)
                     /locus_tag="VFQ10_08625"
                     /pseudo
     CDS             complement(5465..5806)
                     /locus_tag="VFQ10_08625"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
     gene            complement(6000..6323)
                     /locus_tag="VFQ10_08630"
     CDS             complement(6000..6323)
                     /locus_tag="VFQ10_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9965447.1"
                     /translation="MSEHENLTAGGLKSRLDAGERPILLDVREPWEFEAARIEGSTLI
                     PMSRLEERFTELDPACETVVICHQGNRSSYVTRALRRAGFEKVMNLEGGLDAYSSVDA
                     SVPRY"
     gene            complement(6334..6624)
                     /locus_tag="VFQ10_08635"
     CDS             complement(6334..6624)
                     /locus_tag="VFQ10_08635"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HET9965448.1"
                     /translation="MVDHLVFLAVREEASPEEVEDLLSSIRALRDEVPSTVDLSVGED
                     FSGRSGGYTHALFARFEDVAGLREYMEHPAHLTVVEKLDATTTGRIVVDYEF"
     gene            complement(6768..7736)
                     /locus_tag="VFQ10_08640"
     CDS             complement(6768..7736)
                     /locus_tag="VFQ10_08640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9965449.1"
                     /translation="MNYRTLGRTGLKVSEIGYGAWGIAGDAWLGAKDEESLKALNRAV
                     DLGLNFIDTALAYGEGHSERLVGKLVQERDETIHIATKVPPKNRVWPAPEGIPPEEAF
                     PGDYVRECTEQSLSSLGLDTIDVQQFHVWQDDWVGQGDWLEAVEDLMREGKIRAFGVS
                     INDHQASNAVRLIETGAVDTVQVIYNVFDQSPEDRLLPASQEHDVGVIVRVPFDEGSL
                     TGRITPDTTFEEGDFRNDYFRGERKQEVYDRVRVIVSELRASEDEIAEIALRYVLSHP
                     AVSTVIPGMRSVRNVERNMAVGDGKGLPEDQVQVLKNHRWDRNFYA"
     gene            7850..9616
                     /locus_tag="VFQ10_08645"
     CDS             7850..9616
                     /locus_tag="VFQ10_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HET9965450.1"
                     /translation="MRILDERREALVERERELLEKLVSSLKDFGAPSEDIDLVQRAHQ
                     DLEELFLLVIVGEFNSGKSAFVNALLGADIAEEGVTPTTSRITLLRHADEPVEKGRRD
                     EVLEKGYPNEFLREIAIVDTPGTNAIIRHHEELSRGFVPRSDLVLFVTSAERPLTESE
                     RGYLELIRDWGKKILLVINKADLLADESKVEEVRSFVDGGIRSALGLTPPIFFVSALL
                     ARKAKAATNAMERDALMKASGFAELESYVTDLLDEGGRVRLKMESPLGVVGELNRRYR
                     GAVDERLALLEEDFRTAENVESQLELYKEDMGRDFEARIAEIENIVLKMSERGDAWLE
                     ENIRFANIRELFRQEKVSERFEREVVADTEDLVDERVEELIDWMVDRNLKQWRMVVEY
                     VNRRRQADYDGRLIGEMGDSFEYNRGRLIQSVGKNAQDVVQRYDRERESEKLALSIQG
                     AVTQTAALEVGAAGIGGLVVAIATTRFLDVTGIVAAAIVAGYGLVILPNRRRKAREQF
                     RENTDSLRMRLGEVVRRQFEVEANRSVERMREAIAPYTRFVRSEHARMTSAGEDLAAL
                     DAESRALKDEISAPGVGPKTSL"
     gene            9858..11864
                     /locus_tag="VFQ10_08650"
     CDS             9858..11864
                     /locus_tag="VFQ10_08650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET9965451.1"
                     /translation="MADPEMGGKGPYRRAGATQGTPDVRLPYSPGLDGLRAFAVIAVL
                     LYHADLAWIPGGFLGVEVFFVISGYLITALLLAEWRQRGRIDLKTFWLRRARRLLPAL
                     YVLLVVTLAFAVVFLPGEVAGLRGDVLAAFGYVTNWYLIFGQESYFEAVGRPSLLQHL
                     WSLAVEEQFYLMWPPILALGLCIGATRLRRRRVLTVALFGAVASAVTMALLYVPGVDP
                     SRIYYGTDTRATGLLCGAALALLWSPGDKYRTSETRHHRLRLPGRSRFRRRWGWTAPL
                     LLDVVGFAALGALVWFCLNLGEFQPFLYCGGLALVGLATTATIMALVHPHALIGSRFL
                     GSAPLRWVGVRSYSIYLWHWPVFMVTRPDLDVPIDGLPLLALRLTATVVLADLSYRYI
                     ETPIRRGALERAWRTFREARGPLRRRLRLQWAGALVPILASCALLGVAVAQAEPPEKP
                     SYLASMNSVHTVEQSHHASSTAGIDGSRKDRPSLGQESHEQVKAQTTAKKTWDSGRTG
                     TSEFTGSVSAIGDSVMLGAVGGLQKHIQGLTVVDAEVGLQVYAATDTLRYRRALGQLG
                     DVVIVHLGNNGTFTRGQFDEIMRILSGVDRVVFVNVSVPRAWEEPNNEVITEGVERYP
                     NAVLVDWHSASADRSEIFYRDGYHLRPGGQRLYADLISSYLPEE"
     gene            complement(11873..12070)
                     /locus_tag="VFQ10_08655"
     CDS             complement(11873..12070)
                     /locus_tag="VFQ10_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965452.1"
                     /translation="MERRIERERARVEAEYRQDLKSSVLFEKRIRARWREKCLAKNRR
                     IAELEERIVKLERSLGEKEEL"
     gene            complement(12167..12652)
                     /locus_tag="VFQ10_08660"
     CDS             complement(12167..12652)
                     /locus_tag="VFQ10_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9965453.1"
                     /translation="MTMRPYRGFYDMQSEMNRMFDEVFGGLSRRGGRQQLDEPTRWAP
                     VLDVLQEDGDIVVRAELPGVKPEDVDITLHDGVLTISGERKAEEQREGSGYYVRERRY
                     GSFRRSMTLPQGVDESKISARFDGGVLEVRVSGAAAVHEPKRIQIEAGDEGTGEGASG
                     G"
     gene            complement(12744..12950)
                     /locus_tag="VFQ10_08665"
     CDS             complement(12744..12950)
                     /locus_tag="VFQ10_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965454.1"
                     /translation="MAGDQHGRYYAQELAVDQNANIREQIQSALDEGDRHEWHLVGVS
                     DVMAEHVILFWDTERASFGISHRQ"
     gene            13478..13897
                     /locus_tag="VFQ10_08670"
     CDS             13478..13897
                     /locus_tag="VFQ10_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965455.1"
                     /translation="MNPQRISTLVLIFSDVVLALAVWGVAILIRAAWGTSTIAGVLPT
                     NTIAGVLPVAVWVALRAVLGLYPGYGINAVDELRRESYATLGALIIASTFLGLHQGQD
                     VSRSVLIGGFLALLIAAPLVRQSVKWSLKKARLWRAG"
BASE COUNT         2638 a         4403 c         4342 g         2747 t
ORIGIN      
        1 acgcctgaca gcggacggca agatccaccc ttgcctcctc accgacctcg acatccccgt
       61 aaaggacgcc atccgctcgg aagacccgat accggcgatc cacgaagttc tttgggagac
      121 ggcgcgcata aagccagcag cgggtctcac cctccccgag gagtcccgcc accgcaccat
      181 gagccagatc gggggctaaa gagcgaacag ccgggagatg acgctcttcg gcttctcata
      241 ggcgaacacg gcgtgcgtgc cgaagcgtct gatctgggtc ctggggccgt agcggcccca
      301 ggaaaagcca tcgcccggct tcgccaggcg aaggtcgatt atctggaagc tatcgccgag
      361 ctcgcgcgcg aaggccgaga ggatggggtc ctcggacctg aggcaatgtt cgtaggagag
      421 aactccgggg ttcgtccgcc acacgtgggc cagccggtcc tcgtgaacat cgtcgataag
      481 gctctggtgc ggtggctcga acatccggaa cagttcactc aagaactgtt ccagcgagtc
      541 ggcctggtaa aggatcacgg gcgcgtcgtg gcacgcgaag tagataggac cccacctcgt
      601 ggtaccggga tgtagatcca caacccagaa attgccatag ccgtcagccg cgatcgggag
      661 gccatccggg aatgcctgtt caaactcgaa catgagatcc ctaccggtaa aatcgacctg
      721 ttcgagcgtg ccgtagaaac ctcgacacgc gccgagcaac tcctctatct ctggcgggat
      781 acgacacggt agccggttcg cgaactcctg cagttctgtg cgggagaggc cagggaaaag
      841 ctccaacgtg acgacgttgc catcctcgcc gatgagcgtg ccggcctgtg cccggcggat
      901 gatttgcaga ggagtcataa ttgctttctc ggccagaggt ctttgtacga tcatacgatc
      961 gtacaacgcg gttcccaacg cctcctttag tgacgcgcct agtcgggttc tatggtggag
     1021 atgaggccct cgttgttcag cccgtcctgg tagagctcgg ccagctcttt gtggcacttg
     1081 gtggcgacgg ctttgccctt ggtgtgggcc tccatcatga tgtccacggc cctctttacg
     1141 gacatgcgcg gaatcacttt ttgcaacacg gcaacgacgt gctccatcgg cgtgtagtcg
     1201 tcgttgtgca gtatgacctt gtaagggggt tcctgggtga cgcggcgctc tatctctatt
     1261 ccaggcttcg tctcggtcga agaggcggag gggtctgcta cgctatgtaa cgttggtatg
     1321 cttcgcactt tcatctccgg atcgccgaac tggtcacgta aggcaaaata ccgcgacgac
     1381 gccggatgcg caaccccgct tgccctactg aatccggggc aacaccctct cgcctacttc
     1441 gtcgaccgtg cgaaggttac gttccgctat cttcgcggta ctcggtcttt gcggataatc
     1501 ctcagggtcg tcgcgcatga gaggcggcag cccgaccggg ctctttactc tccatttccc
     1561 gagccagtct tcggatacct tctcgggcag ttcctggtgg gagacggcgg cccagagtga
     1621 ggtccaggcg cgcgggacga cgcgccagat gtcgtagcct ccgccgccgg ttgcgaccca
     1681 gcggcctccg cagagctggt gggccaggtc gtgtatccgg tggggcacgt gctcgtagaa
     1741 gcgggtcgtt gcaaagagat gtgtcagggg gtcgagggca tggccgtcgc agccgttctg
     1801 ggagaggatg aggtcaggat cgaaggcacc aagggccatg gggacgacgg cctcgaagca
     1861 ggagatccag gactcgtcgt ccgtaaaggg ctcgagcggc acgttgacgg aaaagccgac
     1921 accgccgtta cggcccttct catcgacccc gccgctaccg gggaagaggt agcggcccga
     1981 ctcgtgcatg gagacggtca ggacgtcggg atcgccgtag aacatccact gcacaccgtc
     2041 gccgtggtgg gcgtcggtgt cgacgtaggc aatgcggatg ccgggatact cttctttgag
     2101 gcgtgagata gcgactccgg cgtcgttgta gacacagaag ccgctggcct tggatcgcat
     2161 ggcgtggtgc agcccgcccg agatgcacat ggcgtggtcc gcctcaccgg ccattacgag
     2221 ccggcacgcg gcgagcacac cccctaccac gcgggcgcag gcttcatgca tgtcagggaa
     2281 gatggggttg tcaggggtcc cgagaccgta atccagcagg tcggacaggg ggccgaggga
     2341 tctcccggcc ttctggacca tgcgcacgta ggtgaggcta tggaccgtcg tcaggtcatc
     2401 gtcggtcgcg ggccccgagg agacgaaggg gtaatcctcc agaagaccca gggcttcgca
     2461 gagctcgagt gccagcctga cgcgcagggg atcgaacgga tggtcaccgc cgaagccata
     2521 gttctccagg gccaggtcgt ggacaatggc agcctggccg ctcaaggcct gggcggccac
     2581 agaacagaat acccggcctc ctcaaggtat ccgactgcct ctgccgtgtc gatagtgtct
     2641 accctgaaca cgacgatgcg ctctctgggc tctcccgcgt cagcgggctc ccgcgtcggc
     2701 cccatagcgg cgctaacgat gtttatgccg cacatcccga agacgccggc cgcgccggcc
     2761 agggaccccg gcctgtcagg aagcgcgatc tccatgcggc tcccaggctc gttggcgccg
     2821 accaggtaga ccagcgcatc catcacgtcc gaggtggtga ggatgccgac aagctcatcg
     2881 tcctcgacta cgggtaggca gccgatccta cgatcccgca tcgtgttcgc cgcctgctct
     2941 atggggtcgt cggggaggac ggagaccaca tcgcttgtca tcacatcttc taccaggatc
     3001 tcctgcaggg ccgtggcccg cgcgggatct ccgagcgcgg gggtggaaga gcgcaggtcc
     3061 cgatccgaga ctattccgac gagtctcccc tctttgagga ccggaaggtg cctgatgcgc
     3121 ctttccctgc acagggcgag cgcagttccc gccgtctcat ccggcgcgag cgtcacgatc
     3181 tcacgggtca tcgagtcctt tacgagaagc atcggataac tccttccctc atcgcattgt
     3241 atagtagcgg atcggcgcag gtaagccctc ggctggtata aaatacctat gcccgagtac
     3301 ctcaaccaac ttctcgcaga cagcgcgatg gcccttgtgc gtgagagctt tacgggcgta
     3361 agcgccccgt ggtggtggga gcggcgtctg gggggcggta tcgaggtctg ccaggagttc
     3421 gacccggaga ccgcaagccg cgagatctcc gctaagacgg gtggcgaagt ttctgaagtc
     3481 cgccgggtca tcgccgagga actcggcctc gacgaccccg agcccgtggt ccttaccttc
     3541 gagatatccg gcgagaccga atcgcgccac gccgctgagt tactcgccga acgttcctca
     3601 gaaccagaag gtctggcttc cgggctctac cgacggatcg aggagatagt cagagcgggc
     3661 tagctccggg tgaatcttta ttcagcgcca caagatgtag tgacgagggt ttcgtgccaa
     3721 ttagtactct atcgaatact ttcgacgtga atcggtccag tgttatgcag gatcgtgtat
     3781 gcggatttcg gagaaagccc cttccacgca cttgggtgaa caggcgaagg gaaaggccga
     3841 gattggttcg tcgtggccct tccctctgtt gctatggtgc cttacaagat gtcgtcacac
     3901 agtgacgtcg ggattgatgt tctggttgag gcggaagagg ttgtcggggt catagcgacg
     3961 cttcaacgac ttgagccggt cgtacttctt ggccccgtag gcctggcgga tccgctcctc
     4021 gccttcctcc atgaggaaat tcacgtagac gctcgtgtgg tggggctcga gggctgacca
     4081 gaagttccta acccactccc gctcttcgtc gaagccctct gccgactcgg tgatgccggc
     4141 aatgttgaac gtatgcccgg cgctgcgtcc gccgaaggct gtctcgtcct cgcccacgcc
     4201 tgcaaccgcc cctccgagct gccagatcac gaaagtactg agcggggact tgatccgcaa
     4261 cgcgtgatcg actgtgatgt cgataacctc gtctgtgagc tgcgcgacgt cgcaggcgcg
     4321 caggtagtac caccacccgt gcggaaacga cggatcgaac atcgcctggt gggtgaggaa
     4381 gggtttgggc acgcacagat cgagcaccgg agatgcgaac actttgagcg gcttcatgac
     4441 cttctccccc tcctccaccg ttcccgaata acagcacacg acgcccacga ccagcttgcc
     4501 gtgcaattct cgggggacga agggaagtgg tggcgccttc ctgtgggcca cgatcgtcat
     4561 gagttcgtcc ggtgcctccg cgatccactc acggtagaag cgtaggacct tcggtgactc
     4621 ctctatgggc cagaagatcg gcccggcgag cacgataggc cccaccgggt tgaggcggaa
     4681 ctcgaagtcg gtgacgatgc cgaagttgcc accccctccc cgcaatcccc agaacagatc
     4741 agcgttctcc gacgcactcg ccttgacaag ttcaccctcg gcagtgacca gatccacgga
     4801 tagaagctga tcgatggtaa gcccgtactt gcgcatcagc cagttgatcc cgccgcctaa
     4861 agcaagtccc gaaagcccgg tgtgcgttac gatgccggac gggaccgcca gcccgaacgc
     4921 ctgtgtctcc cgatcgagct ctccgaggag cactccagcc tgggccctca ccgtgcacgc
     4981 ttccggatcg acgcggatgc ccttcatcag cgagaggtcg ataacgagcc ccccgtcaca
     5041 aaccgagtag ccggggaagc tgtgcccacc gccacgtaca gcaaccagaa ggccattgtc
     5101 cttggcgaac ttcacggccg cgatgacgtc ggccacgccg gcgcaccgcg cgatgagggc
     5161 cgggaaacga tcgatcgacc cgttccacac cctgcgagca tcgtcgtagc cggggtcttc
     5221 aggactgagt agctgacccc tgaacccgtc ccgcaacccc gcgatgtcca gtgctcccat
     5281 tggggaaaat tccgtagcgc ccgccatagt tatcattcct cctctcgctc cccaccggag
     5341 cattagtgcc taagatagca aagtcggggg gatggcgcta cgtctgagaa aacacgttct
     5401 acgaggttgg gtgaatgagc tttccccgag agactgtaga acctcgcggt ccctgacaag
     5461 ctcttcagaa gtgttctcca cactcagccg ggtgttgggt gcagtagggt gtgtcaccgg
     5521 gcaatcgatt gcgcttggag gcgatgaggg aatagattaa gtcctgcagt tgcctgatgc
     5581 caggtaggtt atacacgagc agcgggaggg cacttccgag ggccgcagcc actaaggcgt
     5641 tcaccgcctc ggcaccgcgg tagcgctcgc catcgggtgc tatggcccag gcgctcgcct
     5701 tgcactcttc tagggtgagt cctaccgaag cgggggtgcc acgcttctgg aagggcaccg
     5761 ccgtcacgcg acggttgcga tcgagcgcct tgagtaggcg cactgatcgc gtgcagaacc
     5821 ctcaggcacc gtcgaatacg agagtgagat gttgcggtcg ctcagtaaag gtcacaaacc
     5881 tgattgtacc cctgccatcc cttggagggg caagggccat gggtcacttg ggtgaatagg
     5941 ggcaggaagg gtctgggctg ctagactccg accctcctct agacctaggc cacctcggct
     6001 cagtacctcg gtacagacgc gtccacggag gaataggcat cgaggccgcc ttcgaggttc
     6061 atgaccttct cgaagccggc tcgccggagg gcccgggtga cgtaggagct gcggttgcct
     6121 tggtggcaga tcacgacggt ctcgcatgcg gggtcgagtt ccgtaaaacg ctcctccagc
     6181 cgggacatcg ggatgagagt cgaaccctct atgcgggcgg cttcgaactc ccacggctcg
     6241 cgcacatcga gtagtatggg ccgttctccc gcatcgagcc tgctcttcag cccgcccgcc
     6301 gttaagtttt cgtgttccga catctccaca gacctagaat tcgtagtcga cgacgatgcg
     6361 gcctgtcgtg gttgcatcca gcttctcgac gacggtcaga tgtgctggat gctccatgta
     6421 ctcccgaaga ccggcaacgt cctcgaacct ggcgaagagg gcgtgagtgt atcctcccga
     6481 gcgccctgag aagtcctcgc ccacggagag gtccacagtg ctcggtacct catctctgag
     6541 agcacggata gacgagagga gatcctcgac ttcctcaggc gaagcctcct cccgaacagc
     6601 gagaaagacg agatgatcta ccacagccct gcctcttcgc tccctgtcat gtccgacgaa
     6661 gtctagcaga cgacgcagga gcggattcgc agaggtactg acctactgcg acgcgtgcct
     6721 acttgagaaa accaagagct gccatgctga agtgctgatc tgctgctcta agcgtagaaa
     6781 ttacggtccc agcgatggtt cttgagtacc tgcacctgat cctcagggag tcccttaccg
     6841 tcgccgacag ccatgttgcg ctcgacgttg cggacggagc gcatgccagg gatgacggtc
     6901 gagacggcag gatggctcag gacgtagcgg agtgctatct cggctatctc gtcctcgctc
     6961 gcccggagct cggagacgat gacccgcacg cggtcgtaga cctcctgctt acgttcgccg
     7021 cggaagtagt cgttgcggaa gtcgccttct tcgaaggtgg tatcgggggt gatcctaccc
     7081 gtcagggagc cctcgtcgaa gggtaccctg actatgacgc ccacatcgtg ttcctgggag
     7141 gctggaagga gccggtcctc cgggctctgg tcgaagacgt tgtagatcac ctgcaccgta
     7201 tctaccgcac cggtctcgat gagcctcacc gcgttggatg cctgatggtc gtttatggag
     7261 acgccgaaag cgcggatctt accttctctc atgaggtcct cgaccgcctc gagccaatcg
     7321 ccctgaccga cccagtcatc ctgccagacg tggaactgct gcacgtcgat ggtgtccaac
     7381 ccgaggctgg agaggctctg ctcagtacac tccctgacgt aatctccggg gaacgcttct
     7441 tcagggggga taccctcagg agcaggccag accctgttct tcggcgggac cttggtggca
     7501 atgtgaatgg tctcgtccct ctcctggacc aacttcccta ccagccgctc gctgtgtcct
     7561 tccccgtaag ccagggctgt gtctatgaaa ttgagaccga ggtctacggc tctgttcagg
     7621 gccttcagcg attcctcgtc cttcgcgccg agccaggcat cgccggcgat gccccacgcc
     7681 ccgtagccaa tctccgatac cttcaggccc gttctgccga gcgttctgta gttcacgcag
     7741 cctccatttg tagtgcttgc cggatgatta ttcctctagt taccacgagc ggggcacggc
     7801 gaaaccgtca ggcgtatata atgcttgtat gcagagggag gtataagcgg tgcgtatact
     7861 cgacgagcgc cgcgaggcgc tggtagagcg ggagcgggag cttctagaga agctggtatc
     7921 ctccctcaag gacttcggcg ctccctcgga ggacattgat ctcgtccagc gggcgcacca
     7981 ggacctcgag gagttgttct tgctggtcat cgtaggcgag ttcaactcgg gtaagtcagc
     8041 gttcgtcaac gcgttgctcg gggccgacat cgctgaagag ggcgtcacgc cgacaacgtc
     8101 ccgcatcacc ctattgcgtc acgccgacga gcccgttgag aaggggcgga gagacgaggt
     8161 actggagaag gggtatccca acgagttcct gcgtgagatc gccatcgtcg atacgcccgg
     8221 gaccaacgcg atcatccgcc accacgagga actttccaga ggcttcgtgc cgaggagtga
     8281 cctggtgttg ttcgtcacct ccgccgagcg gcctctcacc gagagcgagc gcgggtacct
     8341 cgagctcatc cgcgactggg ggaagaagat cctgctcgtc atcaacaagg ctgacctttt
     8401 ggccgacgaa tcgaaggtcg aggaggttcg ctcctttgtg gatggcggca tacgttcggc
     8461 tctcggcctg acacccccga tctttttcgt ctcggctctc ctggctcgca aagccaaagc
     8521 ggcgacgaac gccatggagc gtgacgccct catgaaggcg agtggcttcg cagagctcga
     8581 aagctacgtc acagacctgc tcgatgaagg tggaagagtc cggctgaaga tggagagccc
     8641 actcggtgtg gtcggcgagc tgaaccggcg ttacagggga gcggtcgacg agcgcctggc
     8701 gctcctcgaa gaggatttca ggaccgcaga gaacgtcgag tcgcagctcg aactatacaa
     8761 agaggacatg ggcagggact tcgaggcgcg gatagccgag atcgagaaca tagtcttgaa
     8821 gatgagcgag cggggcgacg cctggctgga ggagaacatc cgcttcgcca acatccgcga
     8881 gctcttccgc caggagaagg tgagcgagcg gttcgagcgg gaggtcgtgg cagacaccga
     8941 ggaccttgtg gacgagcggg tggaagagct catagactgg atggtcgaca ggaatctcaa
     9001 gcagtggcgc atggtcgtcg agtacgtcaa ccgccgccgc caggccgatt acgacgggcg
     9061 cttgatcggg gagatgggcg acagtttcga gtacaaccgc ggccggctca tccagtccgt
     9121 cggcaagaac gcccaggacg tggtgcagcg ctatgacagg gagcgcgagt ccgagaagct
     9181 ggcgctctcc atccagggcg ccgtcaccca gaccgccgcg ctcgaagtcg gggccgccgg
     9241 tatcgggggc ctcgtcgtcg ccatcgcgac gacgcgcttc ctcgacgtga cggggattgt
     9301 ggccgccgcg atagtggccg gctacggtct ggtcatcctc ccgaaccgcc ggcgcaaggc
     9361 ccgcgagcag ttccgtgaga acacagactc cctgcgcatg cgcttgggtg aggtcgtcag
     9421 acgtcagttc gaggttgagg cgaaccgctc cgtcgagcgt atgcgggaag ccatagcccc
     9481 ctacacccgt ttcgtccgat ccgagcacgc ccgcatgacc agtgctggag aagacctcgc
     9541 agccctcgac gcggagagcc gtgctttgaa agatgagatc tccgccccag gggttggtcc
     9601 gaagacctct ctctaaaggt cacctcgcac tacagtaccg ggcatccgtt gtagactcgg
     9661 acgccggaag caatccccaa catgagcaac gagccgcggc ctgaagagcg ggaagggccg
     9721 accaaacagt actacttgta gtacttgtag tatttttcta tccggctggc ctgcatagca
     9781 ctttgagact gtgctaggat catcttccta ctttaggagg gcgggaacct acggaacata
     9841 cgctgcgaca accgcagatg gccgaccccg agatgggcgg caagggaccg tacagacgcg
     9901 ccggagcaac gcaaggcacc cctgacgtac gcctccctta cagtccgggc ctggacggct
     9961 tgcgcgcgtt cgccgtcatc gccgtactcc tttaccatgc tgacctggcc tggatccctg
    10021 ggggcttcct cggggtcgag gtcttcttcg ttataagcgg ctacctcata accgcgctcc
    10081 tgctcgcgga gtggcgccag aggggtcgca tagacctgaa gactttctgg ctgcggcgcg
    10141 cgaggaggct cctccccgcg ctctacgtgt tgctagtggt taccctggcc ttcgccgtgg
    10201 tcttcctgcc tggtgaggta gcggggctgc gcggcgatgt gctggctgct ttcgggtacg
    10261 tgaccaactg gtatctgatc ttcggccagg agtcttactt cgaggccgtc gggcgtcctt
    10321 cgctgctaca acatctgtgg tcactcgccg tcgaagagca attctacctg atgtggccgc
    10381 cgattctcgc cctcgggctc tgtatcggag cgacgcgcct caggcgacgg cgcgtgctga
    10441 ccgtcgcgct ctttggcgcc gtggcatcgg ctgtgaccat ggcgctcctg tacgtgccag
    10501 gcgtagaccc gtccaggatc tactacggta cagacacgcg cgctacgggg ttgctgtgtg
    10561 gggccgcgct cgctttgctg tggtcgcccg gcgataagta ccgtacttcc gagacccgcc
    10621 atcacaggct caggcttccg ggccggtccc ggttcaggcg ccgctggggg tggacagctc
    10681 cactcttgct ggacgtcgtc ggcttcgccg cgttgggggc cctggtctgg ttctgtctca
    10741 acctgggtga gttccaaccc ttcctctact gtggcgggct cgcgctggtc gggctcgcaa
    10801 cgacggcgac gatcatggcc ctcgtccacc cgcatgccct cataggttcg cgctttctgg
    10861 ggtcggcccc gctgcgctgg gtcggggtgc gctcttacag catctacctc tggcactggc
    10921 cggtcttcat ggtgacccgc cctgacctcg acgtgccgat cgacgggttg cccctgctcg
    10981 cccttcgcct gaccgcgacg gtcgtgctgg ctgacctctc ctaccgctac atcgagacac
    11041 cgatacgccg cggcgccctg gaacgggcct ggaggacgtt ccgcgaggcg cggggaccgc
    11101 tgcgccggcg gctccgcctg cagtgggccg gggcactcgt gcccattttg gcctcctgcg
    11161 ccctgctcgg cgtagccgtg gcccaggcag aaccgccaga gaaaccctcc tacctggcct
    11221 ccatgaactc cgtacataca gtggaacaat cgcaccatgc gtcctccacg gccgggatag
    11281 acggttccag gaaggacagg ccatctctcg gacaagaaag ccacgagcag gtcaaggcac
    11341 agacgacggc gaagaagacc tgggatagcg ggcgaaccgg tacttccgag tttaccggct
    11401 ccgtgagcgc catcggcgac tcggtaatgc tcggcgccgt cggaggactg cagaaacaca
    11461 tacaggggct cacggtcgtc gacgcggagg tcggactaca ggtctatgct gctaccgaca
    11521 ctcttagata ccgtcgcgcc ttggggcaac ttggggatgt ggtgatcgtg caccttggta
    11581 acaacggtac gttcaccaga ggacagttcg acgagatcat gcgcatactc tccggcgtag
    11641 acagggtcgt attcgtcaac gtgtcagtac cccgcgcctg ggaggagccc aacaacgagg
    11701 tgatcaccga aggggtagaa agatacccca acgccgtgct cgtcgactgg cactcggcta
    11761 gcgcggaccg gtctgagatc ttctacagag acggctacca tctccgcccc ggaggccaac
    11821 ggctctacgc cgacttgatc tcatcgtacc tccccgaaga gtagaccgtg atctagagtt
    11881 cttccttctc cccgaggctc cgctccagct ttacgatgcg ctcctccaac tcggcgattc
    11941 gccggttctt ggccagacat ttctcccgcc agcgggccct gatcctcttc tcgaagagga
    12001 ccgagctctt caggtcctgc ctgtactcgg cctcgaccct ggcacgttct ctttctatcc
    12061 gtcgctccac atcccccata cgctgcaagt gtaaccgctg gcccacgaaa aaccggggcc
    12121 cgatacgggc cccggtccac ctccggcgtg cactccctgc ggtcgcctaa ccgcctgacg
    12181 cgccttcgcc cgtaccttcg tcgccggcct cgatctggat gcgcttcggc tcatgcacgg
    12241 cggccgcgcc agagaccctt acctcgagta caccaccgtc gaaacgggca ctgatcttgc
    12301 tctcatcgac accctgcggc aacgtcatgc tcctcctgaa cgaaccatag cgccgctccc
    12361 gcacataata gccagaaccc tcacgctgct cctcggcttt gcgctccccg gagatggtca
    12421 gtaccccatc gtgcaacgtg atgtctacgt cctccggctt cacaccgggc aactccgccc
    12481 tgacgacgat gtccccgtct tcttgcagta cgtcgagcac cggcgcccac cgggtcggct
    12541 catccaactg ttgcctccca cccctgcggg agaggccgcc gaacacctcg tcgaacatcc
    12601 ggttcatctc gctctgcatg tcgtagaaac cgcggtaagg tctcatggtc atctcgtcta
    12661 cctccttaca ctctccggct ccctactaga gccccccttg cgactgcccc attcaccgaa
    12721 gcgccaagcc atgactggac tggctactgt ctgtggctta taccgaagct ggctctctcg
    12781 gtgtcccaga acaggattac gtgctccgcc atcacgtccg acaccccgac gagatgccac
    12841 tcgtgcctgt cgccttcgtc gagggcgctc tgaatctgct ccctgatatt cgcgttctga
    12901 tccaccgcaa gctcttgggc gtagtatcgg ccgtgctggt cgcctgccat cctatgctcc
    12961 ctttccttag ctcccagatt tcacagtata ggcacgagtt cggccctaca gcaacctcgc
    13021 ctggatggct cgctacggtt ctattcaccc aactttgtag aagggttttc tacagagttc
    13081 gcagcgtcta gcaaggcccc agtaacatca aagataccct aactggggga tgtgcatgcc
    13141 ctgtctgggt ggcatcatgt tcacggcgga aaggtctatg tctgccaact cgattaccac
    13201 ggcaagcgca tgaatgtctg cgatattgtg gggtttgagg acggtatgat caaagcggac
    13261 agggggtact tcgccgagcc cttcgaagcg cccgagtggc gtgcacagtg ggtagaaagc
    13321 ttctagagga gaggctctct tcacccaggc tcgtagggcc tgcataaaaa ctacgcccga
    13381 ggacgacgga tgaggaagat ggttatgtta gtcagtgcgg tggtggctgc ggtagagtta
    13441 gcagtggcag gcccagcgac cgagcggggg tgagcgaatg aacccgcaga ggatctccac
    13501 cttggtcttg atcttctccg acgtcgtgct cgcgctggcg gtgtgggggg tggcgatcct
    13561 gatccgggcc gcctggggca ctagcacgat cgccggcgtc ctccccacca acacgatcgc
    13621 cggcgtcctc cccgtcgcgg tgtgggttgc gctacgggct gtcctggggc tgtacccggg
    13681 gtatggcatc aacgcggtgg acgagctgcg ccgcgagagc tacgcgacgc tgggggccct
    13741 cataatagcc tccacgttcc tcggcttgca ccagggtcag gatgtgtccc gctccgtgct
    13801 cataggtggg tttttggcac tcctgatcgc tgccccgctc gtgcggcagt cggtgaagtg
    13861 gagcctcaag aaagcaaggc tttggagagc tggatgacca gggatagcag ggaaggcaag
    13921 tcgcggtctc acgtccctct cgataccgga agttccgaca ggttcgggga ggcaacgatc
    13981 gaaatgacac ggggtggtga cgaatactgc gaacgtcacc ttgagggttg ttctccgaag
    14041 tagttcgctc agggttacca gaaagagtat gagggcgggg ctatccctga agggaaaagc
    14101 cccgccctgc tggtcgctgg ccgattttgg
//
LOCUS       DASTKM010000208         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1469425, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000208 DASTKM010000000
VERSION     DASTKM010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469425"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFQ10_08675"
     CDS             <1..860
                     /locus_tag="VFQ10_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9965456.1"
                     /translation="LAKAVALAAEDTGIRLLLLPVAYARDGLPRFRDQSVRAFLQRVD
                     DLREWSEVRPLVEIGVGAHSVRAVPRGWLEEIGRYAHREDLRLHIHAGEQPREIEECL
                     AEHGMRPIELLARTGFLGTGTTIVHATHAGERELDLLSEHDASVCACPTTEGNLGDGF
                     LPAQEILERNLRLSVGSDSHVRIDPFEELREIETNARRLSGQRNVLITGNETSPTPYL
                     LRAGWGRARLQAGDRADLIEIDLSHPALSDLEARNLPSALVFGSGSGIVAGTWVDGRK
                     VHARGDTDG"
     gene            853..2088
                     /gene="hutI"
                     /locus_tag="VFQ10_08680"
     CDS             853..2088
                     /gene="hutI"
                     /locus_tag="VFQ10_08680"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HET9965457.1"
                     /translation="MAEVTLLIHDLAAAVTPVGTGPLRGREFGALRVHSPASVAIAGD
                     RIFAVGQPAEILREIPSGRDYESVDGRGKVALPGLVDCHTHPAFLGDRAAEFGLRSSG
                     ATYEEIHASGGGIRSTAAATRAGGERDLRSSVERHLGWMLEHGTTTADAKSGYGLDRE
                     TELKSLRAIEEAGRSSPVDVVPTFLGAHTVPPEFDSAAEYVEFVIHEVLPEAAPLARF
                     ADVFLERGSFEAPEARRYLEACREYGLALRFHADQFSERGAIPLALELGACSVDHLEA
                     TKDEGALLLAKSDVAAVLLPACGLFLDLPLPPARLLADEGAIVALATDFNPGSSYCES
                     LPLVMNLACTRLGFSPAEGLCACTANAAHVLGAEDVGRLSPGYKADVLLLDAPDWRHL
                     AYHLGGDRFAARIKSGRVL"
     gene            2123..2479
                     /locus_tag="VFQ10_08685"
     CDS             2123..2479
                     /locus_tag="VFQ10_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965458.1"
                     /translation="MPATVRIKPELITAHRLRIEMFGLEDEDIENTIRMKGWAWVLAR
                     RGWVYAGEPDFIYRQIREVVIALPDVTFEPDAIEESVKTVLEKARTEEERAEGRLLLH
                     RAFEKTGQLAKAEEFL"
     gene            2724..3062
                     /locus_tag="VFQ10_08690"
     CDS             2724..3062
                     /locus_tag="VFQ10_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9965459.1"
                     /translation="MRFRRLFKRPEPADPFAAFAETYHIRTPRDTPPPKAEPDEPQPV
                     EVGPTRAHQLLGVPLGASRQEVSQAYKKLAFIHHPDRVADMGSEARQYSEERMKEINA
                     AYSELRRRGA"
BASE COUNT          666 a         1095 c         1120 g          595 t
ORIGIN      
        1 ccctggcgaa agccgtagcc ctggcagctg aagatacggg tatccggctc ctcctgctac
       61 ctgtcgccta cgcgcgggac ggcctcccgc gctttcggga ccagtccgtg agagcgttcc
      121 ttcagagggt ggatgatctc agggaatggt cggaagtacg tcctctagtg gagatcggcg
      181 tcggggccca cagcgtcagg gcggtcccgc gggggtggct ggaggagatc ggaaggtacg
      241 cccatcgaga agatctccgc ctgcacatcc acgccggcga gcagccgcgt gagatcgagg
      301 agtgcctggc ggagcacgga atgcgcccca tcgagctgct cgcccggacc ggtttcctag
      361 gtactggcac cacgatagtc cacgctacgc acgccggcga gagggaattg gatctcctct
      421 ccgaacatga tgcttccgtg tgcgcctgcc ctaccacgga ggggaacttg ggcgacgggt
      481 tcctaccggc ccaggagatc ctggagcgta atctcaggct ctccgtcggc tcggactcgc
      541 acgtcaggat agaccctttc gaggagctgc gggagatcga gactaacgcc cgcaggctct
      601 cgggacagag gaacgtcctc atcaccggga acgagacctc acctacgccg tatctcttgc
      661 gggctggatg gggccgcgcc agactgcagg caggcgacag ggctgacctc atagagatag
      721 acttgtccca tcctgcgctc tcagacctag aagccagaaa ccttccctcg gcactcgtct
      781 tcggctcggg tagcgggatc gtcgcaggga cgtgggtgga cggacggaaa gtacacgccc
      841 ggggagacac ggatggctga ggtcacgtta ctgatccacg acctcgcggc cgcggtcacc
      901 ccggtaggta cgggtccgct ccggggcaga gagttcggag cgctgcgcgt ccactccccc
      961 gcctcggtag ccatagcagg ggaccgcatc ttcgcggtcg gccagcccgc ggagatcctg
     1021 cgagagattc catccggcag ggactacgag agcgtggatg ggcgcgggaa agtggcgctt
     1081 ccaggcctcg tcgactgcca cacgcacccg gccttcctgg gagaccgggc agcggagttc
     1141 gggctgcgct cctccggggc tacctacgag gagatccacg cctcaggcgg cggcatccgc
     1201 tcgacagcgg cggcgacgcg ggctggaggt gagagggatc ttcgttcttc cgtggagcgc
     1261 cacctcggct ggatgctgga gcacggtacg acgaccgcgg atgccaaatc aggctacggc
     1321 ctcgaccgcg agacggagct gaagtccctg cgtgcgatcg aggaagcagg ccgcagtagc
     1381 cctgtagacg tagttccaac ctttcttgga gcgcacacgg ttccacccga gttcgatagc
     1441 gcggcagagt acgtggaatt cgtgatccat gaagtgctgc cagaagcagc acccctggcc
     1501 cgattcgccg acgttttcct ggagcgtggc tctttcgagg cgcctgaggc gaggcgctac
     1561 ctcgaggcgt gccgggagta tgggctcgcg ctacgcttcc acgcggacca gttctccgag
     1621 cggggtgcga tcccgctggc cctcgagctc ggcgcctgta gcgtggacca tctcgaagcc
     1681 acgaaggatg aaggtgctct cctgctggcg aagagcgatg ttgcggccgt acttctcccc
     1741 gcttgcgggc tcttcctcga cctgcccctc cctccggcac gcctcctggc cgacgaggga
     1801 gcgatcgtgg cgctcgccac tgacttcaac cccggaagct cctactgcga gtcgctgccg
     1861 ctggtgatga acctggcgtg cacccggctc gggttctctc cagcagaagg cctctgcgcc
     1921 tgcacggcca acgcggcgca cgtgctcggc gccgaagacg tcggcaggct ctcgcccggc
     1981 tacaaagcgg atgtgctcct cctcgacgcc cctgattggc gccacctcgc ctaccatctc
     2041 ggtggggaca ggttcgcggc gaggataaag tcgggacggg tcctgtagtg gccggccaaa
     2101 gcgccgctgt tagactgtct ccatgccagc gaccgtacgt ataaagccgg agctaattac
     2161 ggcccacagg ctcaggatcg agatgttcgg gctcgaggac gaggacatag agaacaccat
     2221 tcgcatgaaa ggttgggcct gggtcctcgc ccgccgaggg tgggtatacg cgggcgaacc
     2281 cgactttatc taccgccaga tcagagaggt cgtcatcgcc ctgccagatg tcacattcga
     2341 gcccgacgcc atcgaggagt ccgtcaagac cgtgctagag aaggcccgta ccgaggagga
     2401 gagagcagag ggacgtctgc ttctccacag ggccttcgag aagacaggcc agctcgccaa
     2461 ggccgaagaa ttcctgtaga cgccgtatct cacaccttcg tatgagatac ggcgtggtcg
     2521 aagcttggcc cgccgactct atataggact tgcttcttca gagcctgtcc gggcgccggc
     2581 acggtgacct ctctaccatt atgggcacga aggcggaata gcttgtaaca cgcttgacgg
     2641 tggcaggggc gcttcgctgc cgcaggcatc atctatcctt gaccaatgtc ccaggccgcg
     2701 aaaatcaagt ataaagggga ggtatgaggt tcaggagatt gttcaagagg ccagaacccg
     2761 ccgacccctt cgctgccttc gccgagacct accacatcag aaccccacga gataccccgc
     2821 cgcccaaagc cgagcccgac gaaccacaac ccgtcgaagt gggtcccacg agggcccacc
     2881 agcttctggg cgtgcccctc ggtgcatctc gccaagaagt gagccaggcc tacaagaaac
     2941 tcgccttcat ccatcaccct gacagggttg cagacatggg ttctgaggcc aggcagtatt
     3001 ccgaggagag gatgaaagag ataaacgcgg cctactcaga gctaaggcgg cgcggggcgt
     3061 aaaggttgcg ggtgatgcgc cgaccgccag gctggtaggc tgcaggtgga ggagccgcta
     3121 gaactcttag atgaattgac actttcttag cgcgggtagc aggccccgtc ccgcattgta
     3181 gggaactctc gtgggcagcc accgtcgggc atgagcggta agggaccgga agtgggtccg
     3241 ccagcctcca agaggttgcc ataccgattt agaagctggc tgccacgatc gactgaaggt
     3301 tgtggtgaag ccctactagc gcttgcagcc tggccaccac cggcactcag caactcgtcg
     3361 catgctatgc cattaccgtc tgggtcgagt cgggaagctc ctggcacgcc ctggtcaagg
     3421 agaccctgca cgaacgcctg cacgaacgcc tgcacgaact gccgctccgc agggga
//
LOCUS       DASTKM010000209         8709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_942806, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000209 DASTKM010000000
VERSION     DASTKM010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942806"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1131
                     /locus_tag="VFQ10_08695"
     CDS             34..1131
                     /locus_tag="VFQ10_08695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9965460.1"
                     /translation="MKVAVIGAGIVGSSVAYWLSEGGAEVVMIDGAEPGSGTTSTSFA
                     WVNANNKLPRDYFELNVAGMREHGRLHAEMGGGWLYSSGNLIWAADEERENLESRVER
                     LRSWSYAAEMLPASAVNEKLEPGAIFPDTEIRIAYFPEESWIDAPALTRTLVEATTRN
                     GAHEHFGNAVRGIEVEGEGVMLQLEDGEVHADAIVNATGAKANTIAEMVGRELPLDVF
                     PGLLVRVAVPGEPLRHLMHTPRINVRPDGPGHVLLHHDSADERLTDDFAGTEDPLCAE
                     LLERARLILQVLDDAEVVEARFGMRPVPADGHSCVGALSGIPGYYEAVTHSGVTLGPL
                     IGRLLAREILTGEVDPLIAPFRANRFPATQT"
     gene            1340..2746
                     /gene="hydA"
                     /locus_tag="VFQ10_08700"
     CDS             1340..2746
                     /gene="hydA"
                     /locus_tag="VFQ10_08700"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HET9965461.1"
                     /translation="MSGLLFSGGTVVSAEGSYRADVLVEDEKIVAVGTNLDTGDAETV
                     DASGKLVMPGFIDAHTHLDMPFGGTVTADDWATGTEAAAAGGTTMIIDFALQEAGGTL
                     AGAFDTWTEKARDKAIIDYGFHVAITDLRGDIKTELPDLAERGVASVKIFMAYKGTPL
                     HTEDDDLFEVLQLSRDAGVLVLVHAENGHAIAKLQEQALSRGDTAPRFHALTRPEPVE
                     AEATNRAIRLAEVAGAPILVVHVSCAPALEAIHLAHERGQTVYAETCPQYFAFEYEDL
                     AREGFEGAKYVCSPPLRDPGNRPALWKGLKFGDLQIFGSDHCSFNYREQKELGKDDFT
                     LIPNGLPGAEERAMTLWTLGVREGKLSENQFVAVLSTNQARIYGAYPRKGALAPGADA
                     DIVLWDPELSTTATVENRHSNVDYTPYEGMEFHGGPAAVYVRGGLVYRDGEVVGERGS
                     GRFVGRSFTAVEGLEVRV"
     gene            2743..4056
                     /locus_tag="VFQ10_08705"
     CDS             2743..4056
                     /locus_tag="VFQ10_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9965462.1"
                     /translation="MTNPDELLRRHKAVLPSWTPLYYERPLELVRGEGFRVWDSEGNE
                     YLDFFGGIVTTISGHAVPEITEAIKSQVDKVFHTSTLYLIENQVRLAEKLIDLAPISG
                     EKKAFFVGSGSEANEAALLFATRYRKSSEIIALRSSYHGSTFMTMGITGQSTWSPTSR
                     SALDVSYARNPYRLRCALCRDEGRCNLLCADDVRAVVEEETTGNVAAFIAEPIQGVGG
                     FVEAPPGYFERVKSILDETGILLISDEVQTGFGRTGEHFWGIDAFDVEPDMIVMAKGL
                     GDGLAIGAVVGRAEVVESVSPNLHLSTFGGNPISTAGALANLNYILDNDLQTNAYEVG
                     AHLKDRLLKMAEDHEIVAEVRGRGLMLALELAGEDLAPDPAAAIRFLEACRTRGLLVG
                     KGGLKANAVRISPPLVITREAADEAADIMEEALAEIGATTPEKVG"
     gene            4056..5294
                     /locus_tag="VFQ10_08710"
     CDS             4056..5294
                     /locus_tag="VFQ10_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HET9965463.1"
                     /translation="MQAEQVDAGRAVDELEELAELTGGPDGARRVAWTEDWEKAREWF
                     RGKLEEIGGITEIETDEAGNVWATAPGDSERAVILGGHIDSVPNGGWLDGALNVVGAL
                     EVMRVLAPQQRPVTLRLVDWADEEGARFGRSLFGSGAAARALRPDDVRSLKDSDGVAL
                     PDALQEHGIDLDRVNEAGRQLENAVAYLELHIEQGPVLERMDLPLGVVLGTFGVERHA
                     VRFTGQQAHSGSTPMEVRRDAFIAAARSAIAFRDDAAARDDVRATTGFVNVSPAIVTA
                     FNGWCEMSLDQRALDPSVLASMLDAAQEATARIATEEGVSVEWERLWRIEPIPFDEDL
                     ISLAEEACNEVAGQAHRLPSGPLHDAAEMARLMPTVMLFVKSLRGLSHTKDEDTPRED
                     LELSVQALHRLALKTMDWAR"
     gene            5534..7060
                     /locus_tag="VFQ10_08715"
     CDS             5534..7060
                     /locus_tag="VFQ10_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HET9965464.1"
                     /translation="MEEFRDLAVNNQAELDRIKSGAMASRLYNEDLAPTGPEQRTWTT
                     YNIAALWIGMSVVITTYLLASSIMAAGMNWWQALLTISLGNVLVLIPMLLNAHAGTKY
                     GVPFPVFVRASFGVRGANFAAVARALVACGWFGIQTWLGGAALDALLTAAWGGWANIT
                     GHTFIAFLVFWAVQLVIILTGVEGVKWFESFSAPLLIGGGVALLIWGFVAGGGVANVF
                     STSAELQQGNVPFWTLFWPSLAANVGYWITLSLNIPDFTRYAKTQRSQVIGQALGLPL
                     TMTAFSFIGIAVTAATIVVFDEAIWDPVALVTRLTGDIPALLILAMFIIAIAQISTNM
                     AANVVSPSFDFSNLAPKYISFRTGGIITAVIGVLSFPWLILETAGAYIFTWLVGYGSL
                     LGAIGAVMIVDYWIVRRRQLDLAELYKMNGSYSYSNGWNWQAIVAVLVSVALVLPGFV
                     KAATTAGLNGGPFPNPTFIESLYNYGLFLTFTVSALVYLGLSMIGGRAAEPAREPEAT
                     "
     gene            7064..7708
                     /locus_tag="VFQ10_08720"
     CDS             7064..7708
                     /locus_tag="VFQ10_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET9965465.1"
                     /translation="MPDASTYALFVAAALALVLVPGPAVIYVVARSVEGGRLTGLVSV
                     LGVELGTLLHVVFAAAGLSAIVVSSAAAFSVVKWLGAAYLVWLGFRQILGRDSGNEEL
                     PLSGGGENRLGVFSQSVLVQVLNPKVALFFLAFLPQFVDPSRGAAWTQVVVLGATLAI
                     LGLFTDGLYALLGGTAGEWIRKQRAGAGLRRAGRYVTGCVYIALGALAAVSGKD"
     gene            7718..>8709
                     /locus_tag="VFQ10_08725"
     CDS             7718..>8709
                     /locus_tag="VFQ10_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HET9965466.1"
                     /translation="MDFGVTFQTNPPASRVIGLTRRAEELGFGYAWTFDSHILWQEPY
                     VIHSQMLSATERIMVGPLVTNPVSRDPSVTASTFATLNDTFGNRTICGIGRGDSVVRV
                     LGRKPTTLRQLEEAMHVIKELAEGREVGYSGVKVSIPWVSDGKLDVWMAGYGPRALGL
                     IGRKADGFILQLADPVILDWTMQHVHDAARDAGRDPKSIKVCVAAPAYVGGDLAHQRD
                     QCRWFGGMVGNHVADLVARYGEDSDVPHALTDYIRARRGYDYSHHGKSDNPSTVFVPD
                     DIVDRFCVLGTVEDHVKKLNELRDLGADQFNVYLMHDAMDETLEAYGEEIIPALG"
BASE COUNT         1606 a         2784 c         2782 g         1537 t
ORIGIN      
        1 gaatgatacg atcctgctcg gtgtggaggc gcgttgaagg tcgcggttat aggagccggg
       61 atcgtcggct cttcggtcgc ctactggctc tccgagggcg gggctgaggt tgttatgatc
      121 gacggcgcgg aaccgggttc cggcacgact tcgaccagct tcgcctgggt caacgccaac
      181 aacaagcttc cccgcgacta cttcgagctg aacgtggccg gaatgcgcga acacgggcgc
      241 cttcacgctg agatgggcgg cggttggctt tactcttcgg gaaacctgat ctgggccgcg
      301 gacgaagaac gggaaaacct cgaaagtcgc gtcgaacgcc tgcgctcctg gtcctacgcc
      361 gccgagatgc tccccgcctc ggccgtaaac gagaagctcg aacctggagc catttttcca
      421 gacaccgaga tccggatcgc atacttcccc gaagaatcct ggatcgatgc tcctgccctg
      481 acgagaaccc tcgtggaagc taccacccgc aacggtgcac acgagcattt cgggaatgcg
      541 gtgcgcggga tcgaggtgga gggcgaaggg gtgatgctgc aactggaaga tggtgaggtt
      601 catgccgatg ctatcgtcaa cgctacgggg gcgaaggcca ataccattgc ggagatggtc
      661 gggagagagt tgccgctgga cgtgttccca ggactactcg tgcgggtcgc cgtccctggc
      721 gagcctctga gacacctcat gcacacaccg cgcatcaacg ttcgccccga cggtcctgga
      781 cacgtcttgc tgcaccacga ctccgctgac gaacggctca ctgacgactt tgccggaacg
      841 gaggatccgc tctgcgctga acttctggag cgggcgcggc tcatacttca ggtactcgat
      901 gacgcggagg tcgtggaggc gcggttcggt atgcgccctg ttccggcgga cggacattcc
      961 tgcgtcggcg cgctctctgg cattccgggc tattacgagg ccgtgacgca cagcggcgtt
     1021 acgctcggcc cgctgatagg gcgtctgctg gcgcgtgaga tcctgaccgg tgaggtggac
     1081 cctctcatcg caccgttccg tgcgaatcgc ttcccagcaa ctcaaacgta gccctctaca
     1141 cacgaccgga gagccagtat cgagactcgc cgcgagaaaa tcccgtacag agtgatgccg
     1201 agatccggag caggatattt tgccggtcag ccaaagcctc ctgatgcccg cggagatcgc
     1261 ccggcttcac gattgacgcg aaggcgcggg gtgtgcgaga gttagccaac acaccgctgc
     1321 caagtggaag gagaacacta tgagcgggct tcttttctcc ggcgggaccg tcgtctcggc
     1381 cgagggcagt taccgggccg acgtgctcgt cgaggacgag aagatagtcg ccgtgggcac
     1441 gaacctcgac actggtgatg cagagaccgt cgacgcctct ggtaagctgg taatgccggg
     1501 cttcatagat gcacataccc atttggatat gccgtttggc ggtaccgtca cggcggacga
     1561 ctgggccacg ggcacagagg cggctgctgc aggcggcacg accatgatca tcgacttcgc
     1621 cctgcaagag gccggaggca ccctcgccgg agcattcgac acgtggaccg agaaggcccg
     1681 ggataaggcc atcatagact acggtttcca cgtcgccata acggacctgc gcggcgacat
     1741 aaagacggag ctgccagacc tcgccgaaag aggcgtcgct tcggtcaaga tcttcatggc
     1801 ctacaaagga acacccctgc acaccgaaga cgacgacctc ttcgaggtac tgcaactctc
     1861 cagggatgca ggtgtcctcg ttttggtcca cgctgagaac gggcacgcca tcgccaagct
     1921 gcaggagcag gctctctcgc gaggtgacac ggccccccgc ttccacgccc tgacccgtcc
     1981 cgagccggtc gaggccgagg ctacgaacag ggccatccgc ctcgccgagg tcgccggggc
     2041 tcctatcctg gtcgttcacg tctcgtgcgc accggccctc gaggctatcc acctcgccca
     2101 cgagcgtggc cagacggtct acgccgagac gtgtcctcag tacttcgcct tcgagtacga
     2161 ggatctggca agagaaggtt tcgagggcgc caagtacgtc tgctccccgc cgttgcgcga
     2221 cccggggaac agacccgcgt tgtggaaagg cctcaagttc ggggacctcc agatcttcgg
     2281 ctccgaccat tgctcgttca actaccgcga gcagaaagag ctcggcaagg atgacttcac
     2341 cctgattccc aacggactac caggcgccga agaacgcgcc atgacgctct ggacgctcgg
     2401 tgttcgtgag gggaagctct ctgagaacca gttcgtcgca gtcctctcca ccaaccaggc
     2461 acgcatctac ggcgcgtacc cgagaaaggg agccctggcc cctggcgccg acgcggacat
     2521 cgtactttgg gaccccgagc tctcgacgac ggcgacggtc gagaaccgtc actctaacgt
     2581 ggactacaca ccctacgagg ggatggagtt tcatggcggc cctgcggcgg tgtacgtgcg
     2641 tggcgggctg gtttacagag acggcgaggt cgtcggtgag cggggctcgg ggaggttcgt
     2701 cgggcggagc ttcacggcgg tggagggact ggaggtgcgg gtatgacgaa cccggatgaa
     2761 ctattgcggc ggcacaaggc ggtgctgccc tcttggacgc cgctctacta tgagaggccg
     2821 ctggagctgg tgcgcggcga gggtttcagg gtgtgggatt ctgagggcaa cgagtacctg
     2881 gacttcttcg gcgggatcgt gaccacgatc agcgggcacg ccgtgcccga gataacagag
     2941 gccataaaga gccaggtgga caaagtcttt cacacctcga cgctgtacct gatagagaac
     3001 caggtcaggc tagccgagaa gctcatagat ctcgccccga tctcgggcga gaagaaggct
     3061 ttcttcgtcg gcagcggttc ggaggcgaac gaggccgcgc tgctcttcgc gacgcgctac
     3121 cgcaaatcca gcgagatcat cgccctccgt tcttcctacc acggctcgac cttcatgacg
     3181 atgggcataa ccggccagag cacctggagc ccgacctcgc gctcggccct cgacgtgtcc
     3241 tacgcccgca acccttacag gctccgctgc gccctgtgcc gagacgaggg gcgctgcaac
     3301 ctcctgtgcg cggacgacgt gcgcgctgtc gtagaagagg agaccacggg taacgtcgcg
     3361 gcgtttatcg ccgagcccat ccagggcgtc gggggtttcg tcgaggcgcc gcccggctac
     3421 ttcgagcggg tcaagagcat tctggacgag acgggaattc tcttgatctc cgacgaggtc
     3481 cagacgggct tcgggcgcac gggcgagcac ttctggggca tcgacgcctt cgacgtggag
     3541 ccagacatga tcgtgatggc caagggcctc ggcgacggcc tcgccatcgg cgccgtcgtt
     3601 ggcagggccg aggtagtcga gtccgtctcc ccgaacctcc atctctccac cttcggcggg
     3661 aacccgatct cgactgcagg cgccctggcg aacctgaatt acattctgga caacgacctt
     3721 cagaccaacg cctacgaggt cggcgcccac ctcaaggacc gcctcctgaa gatggccgag
     3781 gaccacgaga tcgtcgccga ggtccgcgga aggggcctga tgcttgccct ggagctcgcc
     3841 ggggaggacc tcgcgccgga cccggctgcg gccatccgct tcctcgaggc ctgccggacc
     3901 cgcggcttgc tcgtcggcaa gggcggtctc aaggccaacg ccgtccgcat ctccccgccg
     3961 ctcgtcatca cccgcgaggc ggccgacgag gccgcagaca tcatggagga agccctggct
     4021 gagatcgggg ccaccacccc tgagaaggtt ggctgatgca ggccgaacag gtagacgccg
     4081 gccgcgccgt agatgaactc gaagaactag ccgaactcac cggtggtccc gacggcgccc
     4141 gccgcgtcgc gtggacagag gattgggaga aagcccgaga gtggttcagg ggcaagttgg
     4201 aggagatagg aggcataacc gagatagaga ccgacgaggc cggcaacgtc tgggccacgg
     4261 cccctggtga ctcagagcgg gccgtcatcc tcggcggaca catagactcg gtcccgaacg
     4321 gaggctggct cgacggcgcc ctgaacgtcg tcggcgccct cgaagtcatg cgcgtcctcg
     4381 ccccccagca gcgtccggtg actctgcgac tcgtggactg ggccgacgag gaaggggccc
     4441 gctttgggcg cagcctcttc ggctcgggcg cggccgcgag agcgctgaga ccggacgacg
     4501 tgcgcagcct caaagacagc gacggcgtcg ctttgccgga cgcgcttcag gagcacggca
     4561 tcgacctcga cagggtcaac gaggctgggc gacagctcga gaacgccgtc gcctacctgg
     4621 agctgcacat cgagcagggt cccgttctgg agaggatgga cctgccgctc ggggtggtgc
     4681 ttggcacctt cggcgtcgag cggcacgccg tccgctttac gggccagcag gcccattcgg
     4741 gctccacgcc gatggaggta aggagggacg ctttcatagc ggctgcccgc tcggcgatag
     4801 ccttcaggga cgatgcagcg gcccgcgatg acgtacgtgc aaccacgggg ttcgtcaacg
     4861 tcagcccggc gatcgtcacg gcgttcaacg gatggtgcga gatgtccctc gaccagcgcg
     4921 ctctcgaccc ttccgtgctg gcgagcatgc tcgacgccgc gcaggaggcg accgcccgca
     4981 tcgccaccga ggaaggcgtc tccgtcgaat gggaacgcct gtggcgcata gagccgatcc
     5041 ccttcgacga agacttgatc tctctggcag aagaggcgtg caacgaggta gcaggtcagg
     5101 cgcaccggct gcccagcgga cccctgcacg acgctgcgga gatggcgcgc ctcatgccga
     5161 ccgtaatgct cttcgtcaag agcctgaggg gcctcagcca caccaaagac gaggacacgc
     5221 cacgagaaga cctcgagctc tccgtccagg cgctccaccg gctcgcgcta aagacgatgg
     5281 attgggcaag ataagcatgt cagcaagccg cctgccgaag ccgggggctt gtcagcattt
     5341 cagctagtca gctgggtaac ggacggcgag gagacgatct gttcgaagat gcggttcacg
     5401 aagagagtga ttcgcggtcg aacacgggtg tccaaccgtc accaaaaagc tgaaatgctg
     5461 aagtgctgac aagcgtaggc ctgaaaggcc gaagcgtgct tactagttag cgacgaccga
     5521 agggaggagc gacatggaag agttcaggga tctggcggta aacaaccagg cggagttgga
     5581 tcggatcaag tcgggggcaa tggcttcgag actgtacaac gaggaccttg cgccgaccgg
     5641 gccggagcag cggacgtgga cgacctacaa catcgccgcg ctctggatcg ggatgtccgt
     5701 cgtcatcacg acctacctgt tggcttcgag catcatggcc gcagggatga actggtggca
     5761 agccctactg accatctccc tcggcaacgt cctcgttctc atcccgatgc tcttgaacgc
     5821 ccacgccggc accaagtacg gcgttccgtt cccggtcttc gtccgcgcct ccttcggggt
     5881 gaggggggcc aacttcgcgg cagtcgccag ggccctcgtg gcctgcggct ggttcggcat
     5941 acagacctgg ctcggcggcg ccgccctcga cgcccttcta accgccgcat ggggtgggtg
     6001 ggccaacata acagggcaca ccttcatagc tttcctggtc ttttgggctg ttcagctcgt
     6061 catcatcctg accggcgtcg agggcgtgaa gtggttcgag tcgttctccg cgccgctcct
     6121 gatcggcggc ggcgttgcgc tcctgatctg ggggttcgtc gccggtggcg gcgtggccaa
     6181 cgtgttctcg acctcagccg aactccagca gggcaacgta ccgttctgga cgctcttctg
     6241 gcccagcctc gcggcgaacg tgggctactg gatcacgctc tccctcaaca ttccagactt
     6301 tacccgctac gcgaagaccc agcgctcgca ggtcatcggg caggcccttg ggctcccgct
     6361 cacgatgacg gccttctcct ttatcgggat cgcggtcacg gcggcgacca tcgtcgtctt
     6421 cgacgaggcg atctgggacc ccgtcgccct cgtcacgcga cttaccggcg atatccccgc
     6481 cttgcttatc ctcgccatgt tcatcatcgc gatagcccag atctctacga acatggcggc
     6541 taacgtggtc tctccctcct tcgacttctc gaacttggcg cccaagtaca tctcgttcag
     6601 gacaggtggc atcatcaccg ccgtgatcgg cgtgctctcg tttccctggc tgatcctcga
     6661 aaccgccggc gcctacatct tcacctggct cgttggctac ggtagcctcc tcggggccat
     6721 cggggccgtg atgatagtcg actactggat cgtgcgcaga aggcagctcg acctcgccga
     6781 actctacaag atgaacggca gctactcgta ctcgaatggg tggaactggc aggcaatagt
     6841 ggccgtcctc gtcagcgtgg ccctcgtcct ccctggtttc gtcaaagccg ccaccaccgc
     6901 cggactcaac ggtggtccct tcccgaatcc gacgttcatc gagagtctct acaactacgg
     6961 cctgttcctc acctttacgg tgtcggcact ggtgtacctg gggctctcca tgatcggagg
     7021 ccgcgccgcg gaaccggccc gcgaaccgga ggcgacgtag agagtgcccg acgcctccac
     7081 ttacgccctt ttcgtcgcag cggcgctggc cctagtgctc gtgcctggac cggccgtcat
     7141 ctacgtcgtg gcccgcagcg tggaaggagg gcgcctgacc gggctcgtct ccgtcctcgg
     7201 tgtcgagctc ggaaccctct tgcacgtggt cttcgccgcg gcgggactct cggcgattgt
     7261 agtctcttcg gcggccgctt tctccgtcgt gaagtggctc ggcgcggcat acctggtctg
     7321 gctcggcttc aggcagatcc tcggacgcga cagtggtaac gaggagctgc cgctgtcagg
     7381 cggcggagag aaccgcttgg gcgtcttttc acagagcgtg ctcgtgcagg tcctcaaccc
     7441 gaaggtcgcg ctgttcttcc tggccttcct gccgcagttc gtcgatcctt ccaggggcgc
     7501 tgcctggacg caggtggtgg tcctcggcgc cacactcgcc attctcggtt tgttcacaga
     7561 cggcctctac gccctgctcg gtggtacggc tggtgagtgg atcaggaaac agagagcagg
     7621 cgcaggcttg cggcgggccg gacgctacgt cacgggttgt gtctacatag ccctcggcgc
     7681 cctagcggca gtgtcgggta aggattagga ggaaggcatg gacttcggtg tcacgtttca
     7741 gacgaatcca ccggcaagcc gagtcatcgg gctgaccagg agggccgagg aactcggctt
     7801 cgggtacgcc tggaccttcg actcacacat cctctggcag gagccctacg tcatacactc
     7861 gcaaatgctc tccgcaacag agaggatcat ggtcggcccg ttggttacca atcctgtcag
     7921 ccgcgaccct tcggtgacgg cctccacctt cgccaccctc aacgatacgt tcggcaaccg
     7981 cacgatctgc ggtatcggac gcggcgactc ggtggtgcgg gtacttggcc gcaagccgac
     8041 gacgctccgg cagctcgaag aagccatgca cgtcataaag gagctggccg agggacgcga
     8101 ggtcggttac tccggcgtca aagtcagtat cccctgggtc agcgacggaa agctcgacgt
     8161 gtggatggcc ggctacggcc ccagggccct gggcctcatc gggcgcaagg ccgacggttt
     8221 catcctgcag ctcgctgacc ccgtgatcct ggactggacc atgcaacacg ttcacgacgc
     8281 ggcgagagac gctggccgtg acccgaaaag cataaaggtc tgcgtcgcag cccctgccta
     8341 cgtcggcgga gacctggccc accagcgcga ccagtgccgc tggttcggtg gcatggtagg
     8401 caaccacgtg gccgacctcg tcgcacgcta tggcgaagac agcgatgtgc cgcacgcgct
     8461 taccgactac ataagggccc gccggggtta cgactactcc caccacggca aatcggacaa
     8521 cccctcgacc gtcttcgtcc ccgacgatat cgtcgaccga ttttgcgtcc tcggcaccgt
     8581 cgaggaccac gtaaagaaac tgaacgagct aagagatctc ggcgcggacc agttcaacgt
     8641 ctacctcatg cacgacgcca tggatgagac gctcgaagct tacggagagg agatcatacc
     8701 ggcgttggg
//
LOCUS       DASTKM010000210         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_767623, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000210 DASTKM010000000
VERSION     DASTKM010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767623"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFQ10_08730"
     CDS             <1..301
                     /locus_tag="VFQ10_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET9965467.1"
                     /translation="DLYRDHGLVFTSGVGTLINPTNLRRRSFARLLERAGLPQIRFHD
                     LRHTCATLLLSRNVHPKYVQELLGHATVAITLDTYSHMIPGMGNHTARAMEDVFS"
     gene            complement(406..478)
                     /locus_tag="VFQ10_08735"
     tRNA            complement(406..478)
                     /locus_tag="VFQ10_08735"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(443..445),aa:Ala,seq:cgc)
     gene            885..1312
                     /locus_tag="VFQ10_08740"
                     /pseudo
     CDS             885..1312
                     /locus_tag="VFQ10_08740"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            1529..1864
                     /locus_tag="VFQ10_08745"
     CDS             1529..1864
                     /locus_tag="VFQ10_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET9965468.1"
                     /translation="MAPDQPLGCYGKKASAYTERELMDRVVRLEIPRIGDSEDAYGRT
                     LAYVYLDTDEDGEYEHLFNEDLIELGLARTTTFSHAYRREFERLREEAEVRGIGLWSA
                     CPQAEGYPA"
     gene            1891..>2525
                     /locus_tag="VFQ10_08750"
     CDS             1891..>2525
                     /locus_tag="VFQ10_08750"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HET9965469.1"
                     /translation="MLGPRELNRATLERQMLLRRRKLSAVEAIERLVGMQAQAPAPPY
                     IGLWTRLKNFHPDELERLILERRAVRIALMRNTVHLVSARDCLALRPLMQPVLDRGLY
                     ATRANRAHLDGIDVDALVAAGRALLEEEPHTARELGELLQEQWPDRDPASLARAIRHL
                     VPLVQVPPRGLWGKSGPAAHTTAETWLGRPLDPTPSLEKTILRYLGAFGPAT"
BASE COUNT          481 a          819 c          740 g          485 t
ORIGIN      
        1 tgatctctat cgtgatcacg gcctcgtctt cacctctggg gttggcaccc taatcaaccc
       61 cactaacctg cgcagacggt cgttcgcgcg gctcctggaa agggccggac ttcctcagat
      121 acgcttccac gacctgcgtc acacctgcgc gacactgctc ctgagccgca acgtgcatcc
      181 caagtacgtc caagaactct tgggccatgc caccgtggcg atcacgctgg acacctattc
      241 acacatgatc ccagggatgg gaaaccatac cgcgcgtgca atggaagacg tattctccta
      301 aggccacctc gactgtcgtc aaactgtcgt caagaagggc tgcgcctcgt tccagccctt
      361 cttcatccta ctacgtttta cctgcaaatc actacaaaaa gagagtggac ccgaggggac
      421 tcgaacccct gacctccgcc atgcgagggc ggcgctctcc cgactgagct acgggccctg
      481 gagcctcagc aatcctagca gccgggcccc ggattgcaag gtatggagcg aagggtgtgg
      541 cgacaccttc atcggcggct actgtgccac agccgacgat agtgtacgcg gacctgcatg
      601 gatcttctgg agctttggcg cctgagtcct agtaccggaa ttggttgccc ttgctaattc
      661 cggtaccgaa atagcccagc acccagtatc ttagagatat gaaagagaac aggcgcacca
      721 catacaggat ctctgaggct gctcgcgaac tcggcatctc catcgagtgg ctgaggaaag
      781 gtgaggcgag atctcgatga ccgggacctc tacatcctgg ccggcattcg ccctcaagta
      841 cgacaagggc ccttgttcta caagatgaag cgtcagaagg acatgtgaag gtagcgaccc
      901 atgtcgtctt cgcggagtgt tgctggtttg cgacctcggc cgtcttcgac gttcaatacg
      961 caatccccga ggtcctggcc gtaggcgcag cctcactcat ccccgatgcc gactaccccg
     1021 agagctggct cggatatcgg cttggtagag tctccaagga cctcaaccgc ctctttgggc
     1081 accgcagctt cttgcactgc ttgctggcct tggttgtcgt cgttacagtt ctgggcgctt
     1141 gcctatgtgg tggatcgcgg gcaacctggc acccgtagcg gccatcttcg tcggttatgg
     1201 tagtcacctc gtggccgaca tgatgagcgt aggaggagtc caactattct ggcccaaccg
     1261 ggcgatagcg gtcttccccg gccgcgacga ctaccgcgtc gtccccggct cgggttcaga
     1321 gcgtgtgttc gtcatcgtgg ttttcgtact ggcgctcctg ttctacccgg tttcgagggt
     1381 gggcttcgac ggtttgatct accttctcgg tggcgcggat caggccttcg gcaaggttac
     1441 caaggtcaca gatggcgaca ccatctccgt ggaggtctac ggccaggtcc ggcctgtgag
     1501 gctaatcggg gtggacactc ctgagacggt ggccccagat caacccttag gttgctacgg
     1561 caagaaggcc tcagcctaca ctgaacgcga gctgatggac agggtcgtga ggcttgagat
     1621 cccgcgcatc ggagactcgg aggacgccta cggcaggacc ctcgcctacg tctacctcga
     1681 cacagacgaa gacggagagt acgagcatct cttcaacgag gacctgatcg agctgggttt
     1741 ggcacgcaca accaccttct ctcatgccta ccgtcgcgag ttcgagcggc tgcgagagga
     1801 agcagaagtg aggggcatcg gactgtggag cgcgtgcccg caagcggaag gctacccagc
     1861 gtagtgtcct gttggcggga ggctcatgat gtgctcggcc cgcgcgagct caaccgcgcc
     1921 acgttagagc ggcaaatgct gctacgtcgc cgaaagctgt ccgccgttga ggcgatcgaa
     1981 cgcctggtcg gcatgcaggc acaggccccc gctccgcctt acatcggcct gtggacgcgc
     2041 ctgaagaact tccatcccga cgaactggaa cgcctgatcc tggagcggcg cgcggtccgc
     2101 atcgcgctga tgcgcaacac cgtgcacctg gtcagcgccc gcgactgcct tgcgctgcgt
     2161 cccttgatgc aaccggtctt ggaccgcggc ctctacgcta ctcgcgccaa ccgcgctcac
     2221 ctagacggga tagacgtcga cgcactcgtc gccgctggcc gcgcgctcct cgaggaagag
     2281 ccacacaccg ccagggaact cggggagttg ctgcaagagc agtggccaga ccgcgacccc
     2341 gcctccctcg cacgcgccat tcgccacctg gtgcctctgg tccaagtccc tccccggggc
     2401 ctctggggaa agagcggacc agccgcccac accacagcgg agacctggct cggtcgcccg
     2461 ctagacccaa ccccttccct cgagaagacg atattgcgct acctcggggc gttcggcccg
     2521 gccac
//
LOCUS       DASTKM010000211        13618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_62322, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000211 DASTKM010000000
VERSION     DASTKM010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13618
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62322"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /gene="era"
                     /locus_tag="VFQ10_08755"
     CDS             complement(<1..701)
                     /gene="era"
                     /locus_tag="VFQ10_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET9965470.1"
                     /translation="MKGAVARGVENNGLRSGFIAVVGRPNVGKSTLVNRLVGQKVSIT
                     SSQPQTTRSPIRGVRNGPGYQAIFVDTPGSQKPRDTLRARMQEQVVDSLSESDVILLL
                     FDALQASEGLGSGDRYVARLVASSGTPAIVSINKTDLLRTRESALPIVEAVSELGDWN
                     EVFQISAAEGTNVEPLMDSVVDLLPHGPRYFPEGTVTDYPESLVLAEYVREKALNVLR
                     EEVPHAVAVEIEDVE"
     gene            complement(698..1087)
                     /gene="cdd"
                     /locus_tag="VFQ10_08760"
     CDS             complement(698..1087)
                     /gene="cdd"
                     /locus_tag="VFQ10_08760"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET9965471.1"
                     /translation="MDPEALMEAARAAASRAYAPYSNFHVGAAILTGGGTLHSGSNVE
                     NASYGLSICAERNAVAIMATTDPEDREIELVAVFSPNTSPCFPCGACRQVLREFGCKE
                     VVVETGRGLERYPFDQILPNSFSPEDL"
     gene            complement(1099..1842)
                     /locus_tag="VFQ10_08765"
     CDS             complement(1099..1842)
                     /locus_tag="VFQ10_08765"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET9965472.1"
                     /translation="MARKVERPGKGQQGVVRSFEHAYRGMIYAVRTQRNMRFHVVVAV
                     VVLVASLLVGVRKLELAVLVLTILLVFVTELFNTAMEFVVDLATREYHPLAKLVKDVS
                     AGAVLVSSVGALLVGYLVLADDMGPLSLATLESIRRQPAHLTLVTLGVIVLIVLLGKA
                     LTHSPNSFAGGMPSGHAAVAFAGWVAASFIAVQSGAGPYAGLISVISLLMALLVCQSR
                     VESGIHSVYQVLMGAVIGVLVTTAAFQLL"
     gene            complement(1826..2236)
                     /gene="ybeY"
                     /locus_tag="VFQ10_08770"
     CDS             complement(1826..2236)
                     /gene="ybeY"
                     /locus_tag="VFQ10_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET9965473.1"
                     /translation="MSVAVLDEVGHAGLDTERASRLFAAALSTLGLDLERIGEVSVAL
                     VDSDTIRDLNVRFRDRDEPTDVLSFEVDGPYGEMAGEVVICPECADHGMSVEELVVHG
                     ALHLGGMEHGEDFGASEMARLQRAVMEEWRGEEG"
     gene            complement(2284..3867)
                     /locus_tag="VFQ10_08775"
     CDS             complement(2284..3867)
                     /locus_tag="VFQ10_08775"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET9965474.1"
                     /translation="MKIGKRSLKKQPDRKVDEFHRSPTRFELFRAWIERLPRVRLYIG
                     LTALTWLSLTALIGIGPRPLIERIISQGIIDPRDRTRLESLDLLQQADPWLALIGVGI
                     VVAAEMGIAWYFLERFGERILKTNSAIRLVLAASLTVLFTALARLFIELDFNPYLTPL
                     AGLSIIGTMLLGPRLMFLMVVITSVNVGILSGNNFLLTAALLLGSGFAIYTTVRVDSR
                     QRLLKAGLVIAVVMGVVTFAVGLIGGGDPQDALWLGLLGLGNGLLSLMLAMVLLPFLE
                     DAFNILTPMKLLELSDPRHPLLDKLLRNAPGTFSHSMQVGLLAEKAAERVGADALLAQ
                     VGAYYHDIGKMEHPAYFVENQIGQINPHAALSPTLSAKVVKRHVKDGLEIGTAWGLPQ
                     EILDIIAQHHGSTRIEYFYRKALEDASDEKAVRESDFRYSSGLPRSKEAGIMMLADSI
                     EATVKSLPKPTPKRIDDVVADTVRRKLQDGQFDECQLTMHDIHEAGEAIREALIGFLG
                     PRITYPEDQGDARNGKAAS"
     gene            complement(3889..4728)
                     /locus_tag="VFQ10_08780"
     CDS             complement(3889..4728)
                     /locus_tag="VFQ10_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET9965475.1"
                     /translation="MRGNEIVLRGTEGAVERAEVVFDGLVGLAEEGHHPTSETAERLY
                     KMADGGGGREVLGDVILTHRGRRVAPKTRNQKAYTDAIRQHQVVFGIGPAGTGKTYLA
                     VALAVEALKRGEVSRIILTRPAVEAGESLGYLPGDVMAKVDPYFRPLYDALYEMVDPA
                     KFQAHLERGIIEIAPLAFMRGRTLNDAFIILDEAQNTTPQQMKMFLTRLGFGSKMVIT
                     GDITQVDLPKVRISGLQDAKNTLTGVEGVSFVGLRRDDIVRSDLVMRIVDAYEGAGEG
                     DRA"
     gene            complement(4962..5405)
                     /locus_tag="VFQ10_08785"
     CDS             complement(4962..5405)
                     /locus_tag="VFQ10_08785"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET9965476.1"
                     /translation="MTIRDRIQSDTKAAMKQRDRTRVGALRMLGAALKNAEIEAGRPL
                     DEQEEQTILRRQLKQRDESAEAFRKAGREERAASESAEAEIVRTYLPEPLSQDELQAI
                     VDRAIYETGAKGMKDMGTVMNRAMELSEGRAEGRTLSTLARTRLQ"
     gene            complement(5402..5746)
                     /locus_tag="VFQ10_08790"
     CDS             complement(5402..5746)
                     /locus_tag="VFQ10_08790"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET9965477.1"
                     /translation="MSEADCVFCRIVRGEIEAEVVYDEDDVLAIEDINSKAPVHVLII
                     PRQHVANLEEIGALPDTVVKRLFEVASTVAEKLGVRDGGYAVMINNGRDAGQEVFHLH
                     LHVMGGRRLGMP"
     gene            complement(5758..6417)
                     /locus_tag="VFQ10_08795"
     CDS             complement(5758..6417)
                     /locus_tag="VFQ10_08795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HET9965478.1"
                     /translation="MLELPAGEADHVLKVLRGGKGDSVEVVDGAGKLFAARLRGGREA
                     AVTEELAAPDGVDVEISLYQAVPKGGRMDLVVEKATEVGATRIVPLLAERGVVNPREG
                     KVVRWRRVAEAAARQSLRLSVPEVAEPVRFADAVSEVGETGVLLHNSPDLQPVEAVVG
                     APASLFVGPEGGWSGDELRLAEDAGIAFGGLGPYRLRSETAGIVAVTRTLAALESRVE
                     R"
     gene            complement(6483..7619)
                     /gene="dnaJ"
                     /locus_tag="VFQ10_08800"
     CDS             complement(6483..7619)
                     /gene="dnaJ"
                     /locus_tag="VFQ10_08800"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET9965479.1"
                     /translation="MATKRDYYEILGVSREASDAEIKKSYRRLARSHHPDANPGDHEA
                     EERFKELTEAYEVLSNPEARRAYDTYGHQVPRGAGAAGYPGAGDPFGGFQDIFEAFFG
                     DRSFGGSFFGTSTAQGPSRGSDAEVEVEVSLQEAAFGTDREVNVQVVRNCGVCDGVGG
                     TESHVCPTCNGAGSVRTVRESILGQMVTTQTCSTCGGGGRVIDVVCDNCRGSGRISEV
                     QTRRLRVPEGIESGMRLRVPGGGHAGDPGAPPGDLNVRINVKEDQELMRDGEDLIHRM
                     KISFVDAALGAEVDVPTLEGPKQVRVDPGTQPGATLTLRGDGMPRLRRRGRGDLKVVV
                     DVMVPTRLTAEQRDLLERFEATSGEETYNGGGGSFFDRLRGVFR"
     gene            complement(7620..8648)
                     /gene="hrcA"
                     /locus_tag="VFQ10_08805"
     CDS             complement(7620..8648)
                     /gene="hrcA"
                     /locus_tag="VFQ10_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET9965480.1"
                     /translation="MTHLELSERQRELLLRTLEIYISTGAPVGSHALSDELDASSSTI
                     RAELAHLESLGLLDHPHTSAGRVPTDSGYRYYVDALMEANSTDDTAFAALEGYGNVDE
                     LLRGVSEGMSEVTRLLAVVAGPTVLGESLARVDYLPLGEGKLLLVVSMESGASSSAAI
                     TLPVPVEDDELRGIFAALNSWLGGRPLGTDLELGLAARTALSDHDPMTVGAVLEAVGA
                     LGRATERGVFIQGASALLSRLDDLDPGSLSAVIEVFERRRWLLRLMGDALRRSVSSSG
                     VVVSIGTESGFLNLTGTSLVASAYSRHERPYGVVSLIGPKRMDYDAAITTVRSAAEAL
                     TNCLDARF"
     gene            complement(8899..10677)
                     /gene="lepA"
                     /locus_tag="VFQ10_08810"
     CDS             complement(8899..10677)
                     /gene="lepA"
                     /locus_tag="VFQ10_08810"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET9965481.1"
                     /translation="MKHIERTRNFCIIAHIDHGKSTLADRLLDITEAVPERERMDQIL
                     DTMDLERERGITIKAQAVRVLYSREDGEYNLNLIDTPGHVDFAYEVSRAIAACEGALL
                     IVDASQGIQAQTLANLYLAMEHDLEIIPVLNKIDLPIADVPSVTEELVELLGVDEEEI
                     IKISAKTGENVIGVLDAIVDRVPAPHTEHHETRALIFDSLYDPYRGVISLARLFDGEL
                     KKGDRVKAMGSEEHFELLEVGCYSPKPTALSVLGTGEVGYVVAGLKDIEALRVGDTVT
                     RVQRPASEPLPGYAKALPTVFSGLYPTEGDDFERLREALSKLQLNDASLFFEPENSRM
                     GFGFRCGFLGLLHMEIIQERLEREFDLDLIASSPSVKYEVVVEGDVVEVTNPSELPEI
                     FDEIREPVVRATVICPKEYVGAVMGLCHERRGISVGMEYISTRRVQLTYDLPLAEIIT
                     DFFDAMKSRTKGYASYDYEPIGYEPSDLVKVEVLVSGDTVDALSIIVHRDKSYQRGRA
                     IVEKLKELIPRQQFEVPVQATMGKRVIARETVRAYRKDVTAKCYGGDITRKRKLLEQQ
                     KKGKRRMKQVGSVEIPQEAFLAALRI"
     gene            10762..11022
                     /gene="rpsT"
                     /locus_tag="VFQ10_08815"
     CDS             10762..11022
                     /gene="rpsT"
                     /locus_tag="VFQ10_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET9965482.1"
                     /translation="MPAPSKRDKQSVKRGAHNKGVRTRLRNLSKNFYRAVESGDEEEA
                     RRIRDESQREYDKAAGKGMIHPNRAARKVSRLDKTLAANRSE"
     gene            11498..11755
                     /locus_tag="VFQ10_08820"
     CDS             11498..11755
                     /locus_tag="VFQ10_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965483.1"
                     /translation="MGRTRGTGTQRFRVFMWIATALWLIGAIAAVIDRDTLNAVAWFG
                     FAAFGALTASGSTERSRGLAYLAIGLLVAAMAILIGVFLAD"
     gene            11824..12090
                     /locus_tag="VFQ10_08825"
     CDS             11824..12090
                     /locus_tag="VFQ10_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965484.1"
                     /translation="MGEGDHPAKEEAMKRLSEPEGDHELHTVLERMRVHHPHLWEALH
                     RVHFSHEAGPSRLEDWRRAPEGSQEEIWAEHYDEAMGVLAMGLQ"
     gene            complement(12234..12740)
                     /locus_tag="VFQ10_08830"
     CDS             complement(12234..12740)
                     /locus_tag="VFQ10_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9965485.1"
                     /translation="MTSQEIDNISLVELDDAPFREYRDHLVRDYAADKVRAGAWSESE
                     AQIRAAKDVDGLLPEGPATQGHFLYSVREDAADAEVGTVWFALRDSGVGRYVWIYDII
                     INESFRRRGYASRTLELVEARAQELGAKSVELHVFGHNQGAQALYEKVGYSVTSITMA
                     KQVGAADG"
     gene            complement(12737..>13618)
                     /gene="holA"
                     /locus_tag="VFQ10_08835"
     CDS             complement(12737..>13618)
                     /gene="holA"
                     /locus_tag="VFQ10_08835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET9965486.1"
                     /translation="GVEKLRAGRTVEAYDASAVGPETLVSACNSFSLFGEGPFVVLKD
                     LDAWNAAQKAVIVDYLEDPSPGSDLILIGKKLGARERLLSAVKNAGEVHTFDQPTGKA
                     LVRWLVGYAKQMDLDLPEDVAEDLVNRCSGDKMRLLQETEKLALYVGDGTATCDDVAA
                     LCPPDVQSNIFAFVDSLAAGQRDRALALLEDLIATGEPALRLTFMIRRQFQLVARARA
                     LLDRGTPQKDLSSRLKVPPFVARKLEEQRRKLDEEDLERALAQIQNLEGGLKGGSDLS
                     DELQVEMTVLSLSEKAL"
BASE COUNT         2445 a         4298 c         4173 g         2702 t
ORIGIN      
        1 atctctacgt cttcgatctc gacggcaacg gcatgcggca cctcctccct cagcacgttc
       61 agcgccttct cccgcacgta ctcggcgagg accagagact cagggtagtc cgtcactgtt
      121 ccctccggga aatagcgggg accgtgtggc aggagatcta caacggaatc catcagtggt
      181 tctacgttgg tgccttcggc cgcgctgatc tggaacacct cgttccaatc cccgagctcc
      241 gagaccgcct ctactatggg caaggcactt tcacgggtac gcaagaggtc ggtcttgttg
      301 atcgagacga tagccggggt acccgagctg gcgacgaggc gggcgacgta acggtcgccg
      361 ctcccaagtc cttccgatgc ctgcagcgca tcgaagagca acaggattac atccgactca
      421 gagagactgt ccacgacctg ttcctgcatc ctggctcgca gggtgtcgcg gggcttctgg
      481 gagcccgggg tatccacgaa gattgcctgg tatccagggc cgttgcggac gccccggatc
      541 gggctacgcg tcgtctgagg ctgggatgag gtaatggaga ccttctggcc gacgagcctg
      601 ttgaccagag tgctcttgcc gacgttgggt ctgcctacta cggcgataaa gccgctgcgg
      661 agcccgttgt tttcgacgcc acgggctacc gctcccttca cagatcctcc ggagagaagg
      721 agttgggaag aatctggtcg aaggggtagc gctctaaccc ccggccagtc tccacgacga
      781 cctccttgca accgaactcc ctcaagacct gacggcaggc cccgcaaggg aagcatggcg
      841 aggtgttcgg gctgaagacg gcgacgagtt cgatctccct gtcttcaggg tctgtagtgg
      901 ccatgattgc aactgcgttg cgctccgcgc agatcgagag cccgtaggag gcgttctcga
      961 cgttcgagcc tgagtgtagg gtgccgcctc ctgtcaggat tgccgcgcca acgtggaagt
     1021 tgctgtatgg cgcgtatgcc cggcttgcag cggcgcgtgc ggcctccatc agggcctcag
     1081 ggtccacgtt tctccctttc ataagagttg aaaggctgcc gtcgtaacga ggaccccgat
     1141 cacggccccc attagcacct ggtagacgct gtgaatcccg ctctcgacac ggctctgaca
     1201 gacgagcagg gccatgagga gcgagatcac agagatgagc ccggcgtagg gtcccgcgcc
     1261 actctggacg gctatgaagc tcgcggcgac ccaccctgcg aacgcgaccg ccgcgtgccc
     1321 tgaaggcatc ccaccggcga aggagttcgg ggagtgggtc agcgccttgc ctagtaggac
     1381 gatgagtacg atcacgccga gtgtgacgag cgtgaggtgg gccggctggc gccgtatact
     1441 ctccagggtc gccagagaga gcggccccat atcgtccgcc aggactagat agccgacgag
     1501 caaagccccg acgctggaga ccagtaccgc ccccgccgag acgtccttga ctagcttggc
     1561 cagaggatgg tattcccttg tggcaaggtc gactacgaac tccatggccg tgttgaacag
     1621 ctcggtcacg aagaccagga gtatggtcag aacaaggacc gcgagctcaa gcttcctgac
     1681 tccgaccagc aggctcgcca cgaggaccac gacggccacg acgacgtgga agcgcatgtt
     1741 gcgctgggtg cggaccgcgt agatcatacc ccggtaggcg tgctcgaagc tgcggactac
     1801 gccctgctga cctttgcccg gtcgctcaac cttcctcgcc acgccactcc tccatcactg
     1861 cccgctggag cctcgccatc tcactggccc cgaagtcctc cccgtgttcc ataccgccca
     1921 agtgtagcgc gccgtgcacc acgagttctt cgacactcat cccatgatca gcacattcgg
     1981 ggcagatcac aacctcccct gccatctccc catatggccc gtctacctcg aaactgagca
     2041 cgtcggtggg ctcgtctcta tctctgaacc tcacgttgag gtcacggatc gtgtcggagt
     2101 cgacgagtgc cacggagacc tcgccgattc gctccaggtc gagaccgagg gtggaaagag
     2161 cggcggcgaa gaggcggcta gctcgttccg tgtcgagtcc ggcatggcca acctcatcga
     2221 gcacggcgac ggacatggga ggggaattcc tcaggtgacg actctgtccg tggcgtccac
     2281 agcctaactc gccgcctttc cgttgcgagc atcgccttgg tcttcaggat acgtgatgcg
     2341 cggccctagg aaacctatga gcgcctctcg gatggcctcc cctgcttcgt ggatatcgtg
     2401 catggtgagc tggcactcgt cgaactggcc gtcctgaagc ttccgccgta ctgtgtcggc
     2461 gacgacgtcg tcgatacgct tcggggtcgg cttcggcaga gacttcacgg tcgcctctat
     2521 ggagtcggcg agcatcatga tgcccgcctc cttgctgcgg ggcagtccgc tcgagtagcg
     2581 gaagtccgac tcgcggaccg ctttctcgtc ggaagcgtct tcgagggctt tgcgatagaa
     2641 gtactcgata cgggtcgatc cgtgatgctg ggcgattatg tccaggattt cctgcggcag
     2701 tccccaggcg gttcctatct ccaggccgtc ctttacgtgg cgtttgacca ccttagccga
     2761 gagcgtcggg gagagggcgg cgtgcgggtt gatctggccg atctggttct cgacgaagta
     2821 ggcaggatgt tccatttttc cgatgtcgtg gtagtaggcg ccgacctggg ccaataacgc
     2881 gtcggcaccg accctctccg cggccttctc agcgagcagg ccgacttgca tggagtggga
     2941 gaaagtgcct ggcgcgttgc gcagaagttt gtccagcagg ggatgtcggg ggtcggaaag
     3001 ctcgagcagc ttcatcggcg tgaggatgtt gaaggcgtct tccagaaaag gcagcagcac
     3061 catcgccagc atcagcgaga ggaggccatt cccgagtccg aggaggccca gccacagggc
     3121 gtcctgtgga tcaccgcccc cgatcaaacc cacggcgaag gtcacgacgc ccatgaccac
     3181 cgcgatgacc agtccggctt tcagcagccg ctggcgggag tccacgcgca cggtcgtgta
     3241 gatggcgaaa ccagatccta gaagaagcgc cgcggtcagg aggaagttat tgccgctaag
     3301 gatccctacg ttgacgctgg tgatcaccac catcaggaac atgagcctcg gcccgagaag
     3361 catagttccg atgatggaga ggccggcgag cggcgtgaga tagggattga agtcgagctc
     3421 gataaagagc ctggcgagcg cggtgaaaag gaccgtgaga gaggcggcca gcacgagcct
     3481 tatcgccgag ttcgtcttga gtatgcgttc cccgaagcgc tcaaggaagt accaggcgat
     3541 ccccatctcg gcggcgacga ctataccaac cccgatcagg gccagccacg gatcggcctg
     3601 ctggagcagg tcgagcgact cgagccgcgt ccgatcccta gggtctatga tcccctggga
     3661 gatgatgcgc tctatgaggg gcctgggtcc tatacctatg agcgccgtca aggagagcca
     3721 ggtcaaggcg gtaagcccga tatagagcct gaccctgggc aacctctcga tccaggccct
     3781 gaagagctcg aatcgtgtcg gcgagcggtg gaactcgtcg accttcctgt ccggctgttt
     3841 cttgaggctt ctcttcccga ttttcatggt cgctcccggc cctgaggcct aggctctgtc
     3901 gccctcgccc gccccttcgt aggcatcgac tatacgcata acgaggtcgc tgcgcacgat
     3961 atcgtcgcgc ctcagcccta cgaatgatac gccttctacg ccggtgagtg tgttcttggc
     4021 gtcctgcagg ccgcttatgc ggactttcgg gagatctacc tgtgttatgt cgcccgtgat
     4081 gaccatcttg gagccgaagc cgaggcgcgt gaggaacatc ttcatctgct ggggggtagt
     4141 attttgcgct tcgtccagga tgatgaacgc gtcattgaga gttcttcccc tcataaatgc
     4201 caggggtgct atctcgatta taccgcgctc caggtgcgcc tggaacttag ccggatcgac
     4261 catctcgtag agcgcgtcgt agagtggcct gaagtaggga tctaccttgg ccatcacgtc
     4321 gcctggcagg tatccgagcg actcgccggc ctcgacagca ggcctggtca gtattatcct
     4381 ggacacctca cctcttttca gagcctcgac ggcaagggcc acggccaggt aggtcttgcc
     4441 tgtgcccgca ggcccgatgc cgaagactac ctgatgctgg cgaatagcat ctgtatacgc
     4501 cttctggttg cgggtcttcg gcgcgactcg gcgcccgcgg tgcgtgagga tcacatcccc
     4561 cagcacctcc cgcccgccgc ctccgtcggc catcttgtag agacgctcgg cggtctccga
     4621 ggtgggatgg tgcccctcct cagcgagccc gacgaggcca tcgaagacga cctcggccct
     4681 ctccacggca ccctcggtac cgcgcaggac tatttcattc ccccgcacga cgacctcgca
     4741 ctcgacgagc tcctcgacgc gcctcaggac ggagtcgcgg tcaccgaaga cctcgagcgt
     4801 cctgaccggc ggaatgacga gctttacttc tacctggctg ccggttgtgg tttccgtgtg
     4861 gcctcccttc ggtcggcgct tgtcagcatg tcagcacgct ccggcctcaa ggcctccgct
     4921 cgtcagcaat gcagcttttt gatacctgga acctgttgac ttcattgtaa tctggtgcgg
     4981 gctagggtgg agagggtgcg gccttcggca cggccctccg agagctccat ggcacggttc
     5041 atgacggtgc ccatgtcttt catgcctttt gctcctgtct catagatggc cctgtcgaca
     5101 atcgcttgga gctcatcctg tgagaggggt tcaggaaggt aagtgcggac gatctcggct
     5161 tcggccgact cggatgccgc ccgctcctct cgtccggcct tgcggaacgc ttctgctgac
     5221 tcgtcgcgct gcttgagctg gcgcctgagg atggtctgct cttcctgctc gtcgagcggc
     5281 ctccccgcct ctatctcggc gttcttgagc gcggcgccga gcatacgtag ggcgccgacg
     5341 cgggtcctgt cgcgttgctt catggcggct ttggtgtcgc tctgtatgcg gtctcttatg
     5401 gtcaaggcat tcccagcctc ctgccgccca tgacgtgcag gtggaggtgg aagacctcct
     5461 gccccgcgtc gcggccgttg ttgatcatga cggcgtaccc cccatctctt acgccgagct
     5521 tctcggcgac ggtgctcgcc acttcgaaga gccgcttgac tacggtgtcg ggcagggccc
     5581 cgatctcttc caggttcgcg acgtgctgcc tggggatgat gaggacgtgg acgggtgcct
     5641 tgctatttat gtcttcgatt gccagcacat cgtcctcatc gtagaccacc tcggcctcta
     5701 tctcccctcg aactattcta cagaatacgc aatcggcctc gctcacggcc acctcctcta
     5761 acgttcgacc ctgctctcca acgcggccaa cgtacgcgtc accgcgacga taccggcggt
     5821 ctcgctgcgg agcctgtacg gtcccaaccc gccgaaggcg atgccagcgt cctcagcaag
     5881 cctcaactcg tccccgctcc atcctccctc cgggcctaca aagaggctag cgggagcccc
     5941 gacgacggcc tcgacaggct ggagatcggg agaattgtgg aggaggacac ctgtctctcc
     6001 cacctccgag acggcgtccg cgaatcgtac gggttcggcc acctcgggca cgctgagcct
     6061 cagcgactgg cgcgccgccg cctcggccac ccgccgccag cgcacgacct tcccctcgcg
     6121 cgggttaacg acaccacgct cggcaaggag cggcacgata cgcgtagcac cgacctccgt
     6181 agccttctcg acaacgaggt ccatccttcc acctttgggc accgcctgat agagtgagat
     6241 ctccacgtcc accccatctg gcgctgccaa ctcctctgtg acggccgctt ctctcccacc
     6301 acgcaacctg gcagcaaaca gctttcctgc accgtcaact acttcgacgg aatctccttt
     6361 cccaccacgc aataccttga gaacgtggtc tgcctcccca gccggcaact ccagcacgcc
     6421 gcctagctcc agtacggagt cgacgaagat tcgggttatc ggcgtgcgcg taacgaagcc
     6481 tcctaccgga agactcccct gagacggtcg aagaaagagc ctcccccgcc attgtaggtc
     6541 tcttccccgc tggtagcctc gaacctctcg agtaagtccc gctgctccgc cgtcagccgc
     6601 gtcggtacca tgacgtcgac gacgaccttg aggtccccac gcccacgccg ccgcaaccgc
     6661 ggcatcccat cccccctgag cgtcagcgtc gcacctggct gggtgccggg atccaccctc
     6721 acctgtttcg gcccttcgag cgtcggtaca tccacctccg ccccgagcgc cgcatcgacg
     6781 aagctgatct tcatgcggtg gatcaggtct tccccatctc gcatgagctc ctggtcttct
     6841 ttgacattga tcctcacgtt gaggtcccct ggtggcgcgc ctggatctcc cgcgtgtccc
     6901 ccacctggga ccctgagcct catgccgctc tcgatgccct caggcacccg cagccgcctg
     6961 gtctgtacct cggagatcct cccgctgcct cgacagttgt cgcacactac atcgatgacg
     7021 cggccacctc cgccacaagt cgagcaggtc tgggtcgtca ccatctgccc gaggatgctc
     7081 tcccgcaccg tccttacaga gccggcgccg ttacaggtgg gacagacgtg cgactccgtc
     7141 cctcctacgc cgtcgcagac tccgcagttc ctgacaacct gaacgttgac ttcacggtca
     7201 gtcccgaacg cggcctcctg tagggagact tctacttcga cctcagcgtc actaccacga
     7261 ctcgggccct gggcggtgga ggtaccgaag aaggagccgc cgaaagagcg gtcgccgaag
     7321 aaagcttcga agatgtcctg gaagcctccg aacgggtctc ccgcgccggg gtagcctgcc
     7381 gctcctgcgc cgcgcggcac ctggtggccg tatgtatcgt aggcgcgtct ggcttccggg
     7441 ttcgagagta cttcgtaggc ctcggtgagc tccttgaagc gttcttcggc ttcgtggtcg
     7501 cctgggttgg cgtcggggtg gtgggagcgg gcgaggcgac ggtaagattt cttgatctcc
     7561 gcgtccgaag cttccctgga cacgcccaga atctcgtagt aatcgcgctt ggttgccaac
     7621 tagaacctgg cgtccagaca gttggtcagg gcctccgcag ccgagcggac cgtcgtgatg
     7681 gcggcgtcgt agtccatccg cttggggccg atcaggctga caaccccgta cggccgctcg
     7741 tggcggctgt aggccgaggc cacgaggctc gtgcccgtca ggttaaggaa gccgctctcc
     7801 gtgcctatgg agacgacgac cccgctgctg gaaacggagc ggcggagggc gtcgcccatc
     7861 aggcgcagca gccagcgccg gcgttcgaag acctcgatca cggccgagag gctaccaggg
     7921 tcgaggtcgt ccaggcggct caggagggcg gacgcaccct ggatgaagac cccgcgctcc
     7981 gtcgcccttc ccagcgcacc tacggcttcg aggacggctc ccacagtcat cgggtcgtgg
     8041 tccgagagcg ccgtgcgggc ggcgaggccg agctccaggt cggtgccgag agggcgccct
     8101 cccagccagg agttgagagc tgcgaagatg ccgcgcagtt catcatcctc cacggggacg
     8161 ggcagcgtga tcgcggcact agaagaggcg ccgctctcca tcgatacaac gagcaggagc
     8221 ttgccttcgc ccaggggcag gtagtcgacg cgggcaagcg actcaccgag gacggtgggg
     8281 ccagcgacca cggccagaag gcgggttacc tcgctcatac cctccgagac gccccgcagc
     8341 agctcgtcca cgttgccgta accttcaagg gccgcaaacg ccgtgtcgtc cgtagagttc
     8401 gcttccatca gggcgtcgac gtagtagcgg tagccactgt ccgtcgggac ccttccggcc
     8461 gacgtgtgcg gatggtcaag caggcccagg gactcgaggt gggccagttc cgcacggata
     8521 gtggagctgc tcgcatccaa ctcatcggac agggcatggc taccgacagg ggcgcccgtg
     8581 gagatgtaga tctccagcgt cctcagcagt agctcgcgtt gcctctctga gagctcgagg
     8641 tgcgtcatat cgcaggtgat tttaacatgg gtgcgggtgg tcccaaatca gcttgtcagc
     8701 acgcgccctg tcgggcgttt gtcagcattt cagcacgctc aggctgacgc cttcgcttgt
     8761 cagcccgcct tcggcggctt gtcaggactt cagcaagtcg tccgctagct tcgtcacagg
     8821 cagcgattcc tcgtgcgcgg caaggagagg ccggaaaggg agaagctgac aagcggaggt
     8881 cctaagggcc tgagcgtgct agatacgcag cgcggccagg aaagcctcct gcggaatctc
     8941 cacgctacct acctgcttca tgcggcgctt gcccttcttt tgttgctcta ggagcttgcg
     9001 cttgcgggtt atgtcgccgc cgtagcattt ggcggttacg tcttttcggt aggcgcgcac
     9061 tgtctcgcgg gcgatcacac gcttgcccat ggtagcttgc actggcacct cgaactgctg
     9121 gcgtgggatc agctctttga gcttctcgac tatggcgcgt ccgcgctggt aggacttgtc
     9181 gcggtggacg atgatcgaga gcgcgtcaac agtgtcaccg gagacgagga cctcgacctt
     9241 caccaggtcg gacggctcgt agccaatcgg ctcgtagtcg taggaggcgt agccttttgt
     9301 gcggctcttc atggcgtcga agaagtccgt gatgatctcg gccagcggca ggtcgtaggt
     9361 gagctgcacc ctgcgggtcg agatgtactc catcccgacg gagatcccgc gtctctcgtg
     9421 gcatagtccc atcacggccc ccacgtactc cttggggcag atcacagtgg cccgcactac
     9481 gggctccctg atctcatcga agatctccgg tagctcgctc ggattggtga cctcgacgac
     9541 gtcgccttct acaacgacct cgtacttcac actcggagag gacgcgatga ggtcgaggtc
     9601 gaactcacgc tcgagccgct cctggatgat ctccatgtgc aacaacccca gaaaaccgca
     9661 cctgaaccca aagcccattc tggagttctc gggttcgaag aagagcgagg cgtcgtttag
     9721 ttgcagcttg gagagggcct ctctgagccg ttcgaagtcg tcgccctccg taggatatag
     9781 gccagagaag acggtaggca acgccttcgc gtagcctggc agcggttcag aggcaggcct
     9841 ttgcacccgc gtgaccgtat cgccgacgcg cagggcttcg atatctttga ggcctgctac
     9901 tacatatcct acctcgcctg ttccgagtac agagagagcc gtgggtttcg gtgagtagca
     9961 accgacctcc agaagctcga agtgctcttc gctacccatc gccttcaccc tgtcgccctt
    10021 cttcagttct ccgtcgaaca ggcgggccag tgagatgacg ccgcggtaag ggtcgtagag
    10081 ggagtcgaag atgagggccc gcgtctcatg gtgttcggta tgcggcgcag ggacgcggtc
    10141 cacgatcgcg tcgagcaccc cgattacgtt ctcgccggtc ttggcggaga tcttgatgat
    10201 ctcctcctca tctacgccga gcagctccac gagctcttcc gtgacggacg gaacatcggc
    10261 tatcgggaga tctatcttgt tgagcacggg tatgatctcg aggtcatgct ccatcgccaa
    10321 ataaagattt gcgagcgtct gggcttgtat gccctggctc gcgtctacga tgaggagtgc
    10381 gccttcacag gccgctatgg cgcgcgagac ctcgtaggcg aagtcaacgt ggccaggcgt
    10441 gtcgatcagg ttcaggttgt actcgccgtc ttccctgctg tagaggacac gcacggcctg
    10501 ggccttgatc gtgatgccac gttcacgctc caggtccatg gtgtcgagga tctggtccat
    10561 ccgctcgcgt tcggggacgg cctcagttat gtcgagtagc ctgtctgcga gggtactctt
    10621 gccgtggtct atgtgcgcta ttatgcagaa attgcgggtg cgctcgatgt gtttcaccgg
    10681 gagattctac catgattgcc cctttgctct acttgtgtta cattctccgg gtccggtaag
    10741 tattagatcc gaggagccag cgtgcccgca ccatcgaaac gcgacaagca gagcgtcaag
    10801 cgtggcgccc acaacaaggg cgtcaggacc cgccttcgca acctctccaa gaacttctac
    10861 agggctgtcg agtcggggga cgaagaggag gcccgccgca tcagggacga gtcccagaga
    10921 gagtacgaca aggccgcagg caaaggcatg atccatccga acagggctgc ccgtaaggtc
    10981 agccgcctgg acaagacact cgcggccaac cggagcgagt aaagctccct cccgaacctg
    11041 gacaaggtcg ttccctgggg ccagcgaagg gcgaatacgt caggatgttc gcctcgacgg
    11101 caggcgacct ccggagcaag atcctcgact acgccgcggg tccgccgagc ttgaacgctg
    11161 agcttactgc ggatggccac aaggtgactt cctgcgatcc tatctaatta ccttacagcc
    11221 gaagagatac acgccctaat cctggcgacc cgcgaagaga atggtggcca acgtgcgggt
    11281 ggccagggct tcgttctctg tagacatact cgtctcctcc ctttcaggcg caccctcgtg
    11341 gggtcgatct cttcgaccgg tcagcatgat gccatcgaac ccagcccggt ggcaacagtc
    11401 gaaagcgttt ctctcttagg cgccgcagtg aatagcggga ggggccggaa gatgaaatac
    11461 ttattgtgtc ccagccaggt acccggaggg ttagaaaatg ggcagaacta gaggaacagg
    11521 cactcaacgc ttcagggttt tcatgtggat cgccacagcg ctgtggctga tcggggctat
    11581 tgccgcggtc atcgatcgcg atacgctgaa cgccgtggcc tggttcggct tcgccgcgtt
    11641 cggggccctg acagcaagcg gtagcacgga gcggtccagg ggattggctt acctggccat
    11701 agggctcctg gtcgctgcca tggcgatcct gatcggtgtg ttcctcgcgg actaggcgct
    11761 gtgatggaga tgccctgtgg cctatctaca ctcaaatgcg atagtcaaag aagggagaag
    11821 accatgggcg aaggtgacca cccggccaaa gaggaggcta tgaagcggct ctcagagcct
    11881 gagggtgacc acgagttgca caccgttttg gagcgcatga gggtgcacca cccgcacctt
    11941 tgggaggcgc tccaccgggt gcacttctca catgaggccg gtccctcgag gctcgaggat
    12001 tggcgtaggg cgcctgaggg tagccaggag gagatctggg ctgaacatta tgacgaagcc
    12061 atgggcgtac tggcgatggg gctgcagtag cccaattctg gcgcagtgga ttcccgttta
    12121 cgcgggaatg acggttatgc ttggagcaag gcagttgcgc tcgtccggtt cgtacaccgc
    12181 gaagagtcat cttgcgggtt ccgtatcact ccatatcgag agcttcgagg aatttagccg
    12241 tctgcggcac ctacctgctt ggccatggtg atgcttgtta cgctatagcc caccttctcg
    12301 tagagagcct gcgctccttg gttgtggccg aacacgtgta gctccacgct tttggcaccg
    12361 agctcttggg ccctggcctc cacgagctcc agcgtgcggg aagcgtagcc tctgcgcctg
    12421 aagctctcgt ttatgatgat gtcgtagatc cagacatacc ttccaacgcc ggagtccctg
    12481 agcgcgaacc atacggtccc aacctcggcg tcggcagcat cttccctgac cgaatagagg
    12541 aagtggccct gagtagcggg gccttctggc agcagtccgt ctacgtcttt ggccgctctg
    12601 atctgtgctt cggactcgga ccaggcgccc gcgcgaactt tgtccgccgc gtaatcgcgc
    12661 accagatggt ctctgtactc tcgaaatggc gcgtcgtcga gttcgacgag gctgatgttg
    12721 tctatctcct gagaggtcat agggctttct cggacaggct tagcacggtc atctccacct
    12781 ggagttcgtc ggagagatcg ctccctcctt tgaggccgcc ttccaggttc tggatctggg
    12841 ccagagcgcg ttccaggtcc tcctcatcga gcttgcggcg ctgctcctcg agcttgcgcg
    12901 ccacgaatgg cgggaccttg agcctgctcg agaggtcttt ttggggagtg ccgcggtcca
    12961 gaagggcgcg ggcgcgggcg acgagctgga actggcggcg gatcatgaac gtgaggcgca
    13021 gcgccggctc gccggtggcg atcaggtcct cgagcaacgc tagtgctcta tctcgttgtc
    13081 cggcggccag ggagtcgacg aaggcgaaga tattggactg gacgtccggt gggcacaggg
    13141 cggccacgtc atcgcaggtg gctgtgccat caccgacgta gagcgccagc ttctccgtct
    13201 cctgcagcag acgcatcttg tcgcccgagc agcgatttac caggtcttcg gcaacgtcct
    13261 cggggagatc caggtccatc tgcttggcat atccgacgag ccatctgacg agggccttgc
    13321 cagtaggctg atcgaaggta tggacctctc ctgcattctt gaccgcagag agcagcctct
    13381 cacgggcacc gagcttcttg ccgatcagga taaggtcaga tccaggcgaa gggtcctcga
    13441 ggtagtcgac gatcaccgcc ttctgggccg cattccaggc gtcgaggtcc ttcaagacga
    13501 cgaacggtcc ctccccgaaa agggagaagg agttgcaggc cgagacgagg gtctcagggc
    13561 caacggcaga ggcgtcataa gcttcgaccg tccttccggc acgcagcttc tcgacgcc
//
LOCUS       DASTKM010000212         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1121104, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000212 DASTKM010000000
VERSION     DASTKM010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121104"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1306
                     /locus_tag="VFQ10_08840"
     CDS             44..1306
                     /locus_tag="VFQ10_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like protein"
                     /protein_id="HET9965487.1"
                     /translation="MTDAPIVVENREELIFLLSEASELEHMVMLAYLFAAFSLKTDAG
                     EGLTEEQLGAVRRWERVISEVAAQEMLHLASASNLLTAIGGAPHFSRPNFPQPAKFYP
                     PGFELALMPFSEQALGSFVFYERPEGMEHDAPELELASEVIAPLIPGGVVVPHEQEFA
                     TVGHLYRGIEQGFRRLVEKYGEQRVFIGPPEAQATWRYFWWPELAPVTDLASAQAAIE
                     TIVEQGEGARSDRDDAHFGMFLRVLVEYLEFKRRDPDFEPARPVTAAWVRPPVDAKLP
                     NPTLITDPTTAGVADLFNATYEVMLQMLVRFFAHTEETEEELQTLSRTAVGAMSLLIK
                     PLGQLLTTLPVGAAYPGKSAGPNFEFYRTSYLLPHRHAAWVLMHERLLELAVYCARLG
                     ARPSAPPELTTIEETFRKLARALEQYIG"
     gene            complement(1664..1903)
                     /locus_tag="VFQ10_08845"
     CDS             complement(1664..1903)
                     /locus_tag="VFQ10_08845"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HET9965488.1"
                     /translation="MKGISDLWRTDKVVRNARDAFVTEHMEIVAYEVLERLAKRVGDT
                     QTAEVAREIRAEEGAMAERLASKWDSFLDMTLRNE"
BASE COUNT          397 a          667 c          651 g          395 t
ORIGIN      
        1 ataaagtacg atctacattc caagtaaggg aaggacgaga ccgttgacgg acgccccgat
       61 cgttgtcgag aaccgggaag agctcatctt tctgctctcc gaggcctcag agctggagca
      121 catggtaatg ctcgcgtacc ttttcgccgc cttcagtctc aaaaccgacg ccggcgaagg
      181 gctgaccgag gagcagctcg gggcggtcag acggtgggag cgggtgatct cggaggtcgc
      241 cgcccaggag atgctccacc tggcctcggc gagcaacctg ctcacggcca taggcggtgc
      301 cccacacttc agtcggccga acttcccgca gccggcaaaa ttttatccac cgggattcga
      361 gctggctctc atgcctttca gcgagcaggc cctggggagc ttcgtcttct acgagcgacc
      421 cgagggcatg gagcacgacg cgccggagct ggagctcgcg agcgaggtga tagcgcccct
      481 catccctggc ggcgtcgttg tgcctcacga gcaggagttc gccaccgtag gccacctcta
      541 tcgggggatc gagcaaggct tcaggcgcct ggtcgagaag tacggtgagc agagagtctt
      601 catcggaccg cccgaagccc aggccacctg gaggtacttc tggtggcccg agttggcccc
      661 ggtcaccgac ctcgcgtcgg cacaggcggc gatcgagacg atcgtcgagc agggcgaggg
      721 ggctcggagc gacagagacg acgcccactt cggcatgttt ctccgggtcc tggtggagta
      781 cctcgagttc aagaggcggg atccggactt cgagccggcg agaccggtga cagcggcatg
      841 ggtgaggccg ccggtcgacg cgaagctgcc caaccccacc ttgataaccg accccaccac
      901 ggccggggtg gctgaccttt tcaacgccac ctacgaggtg atgctgcaga tgctggtccg
      961 cttcttcgcc cacactgagg agaccgaaga ggaactacag acgttgagca ggaccgccgt
     1021 aggcgcgatg tccctcctca tcaagccttt gggacaactt ctcactaccc tgccggtggg
     1081 agcagcgtat ccgggcaaga gtgcggggcc gaatttcgag ttctaccgca cgagctacct
     1141 gctgccgcac cgccacgccg cctgggtgct tatgcatgag cggctcctcg agctggcggt
     1201 ttactgcgct aggctgggcg cccggccgtc cgctccacca gagctgacga cgatcgaaga
     1261 aacctttcgg aagctcgccc gcgcgctgga gcaatacatc ggctgagaaa acggaaggcg
     1321 ctagcgcagg tgcatccacg agcgcattta cctctgtcct acaagaaagc gagctgcgag
     1381 gcgacgctat gggggttgcg gagggacccg tacgaatcat ccgcgacagt tcggagtttg
     1441 ggaagctccg ctcgggtggg gtttcggttg ctccgcacac taaccgtgcc tggacccagc
     1501 acttccagcg ggccgctgcg gtagtggtgg acagcggcga gcagcgtcac acgcagccat
     1561 tgtggcacgc tcaatcgcct tccgacggaa ggtgctgtat ccctgaagga cctgctgcgc
     1621 cattcgcgaa ggcttgtgca cgcacggttc tctaaacgcg tgtttactca ttgcgtaacg
     1681 tcatatccaa gaagctatcc cacttggagg ctagcctctc ggccatcgcc ccctcctcag
     1741 cgcggatctc acgcgctacc tcggcggttt gagtatcgcc cacccgcttg gccagtcgct
     1801 ccagcacctc gtaggccacg atctccatgt gctcggtgac gaaggcgtcc ctggcgttcc
     1861 ttactacctt gtcggttcgc cagagatcgg agataccttt aatctgtgcc gccgtatcgc
     1921 cccgaggtcc ttgcccatag agatcaggct tagcccacct aacgccctga ggcgttcgtt
     1981 gagtcgccgc tcatggcggc gagtctccac ctcgtgctct tcaaacatcg ccgcgagctc
     2041 cgcatcctcg gttgtgagga tgatggaatc gagcaacatg agaatctatt gctccatggc
     2101 gtgaacgttc
//
LOCUS       DASTKM010000213        10329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1121613, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000213 DASTKM010000000
VERSION     DASTKM010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10329
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121613"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFQ10_08850"
     CDS             <1..296
                     /locus_tag="VFQ10_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9965489.1"
                     /translation="RFMVNVNIYEPQDEQDMELHRIDPEVSESQVERLKALKERRDDD
                     RVKQCLEELKEVARGEGNTMYPILEAVRAYATVQEISDALKDVFGDYRETPVI"
     gene            410..724
                     /locus_tag="VFQ10_08855"
     CDS             410..724
                     /locus_tag="VFQ10_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965490.1"
                     /translation="MEARVVDLRILPTEAKEVVRIYRDSVLPAARRQQGFKGALLLTD
                     DDTGVGISISLWETEEDREEGEANGFYKQQIRKFSDLLTETPVRKHYNVGVLHYDASV
                     LA"
     gene            841..1845
                     /locus_tag="VFQ10_08860"
     CDS             841..1845
                     /locus_tag="VFQ10_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET9965491.1"
                     /translation="MAIRETSAGHEALGLDADDLRRMHYYMLLARRVDERSWTLNRQG
                     KAAFVISCQGQEAAQVGAAYNLRPGNDFLYPYYRDSGMTLLLGQTPRDQFLSLLGKKE
                     DPNSGGRQMPGHYSSRKLHIVTSSAPVGIQYPQAAGTALAYKIRGEDGVVLVCGGEAS
                     TSGGDWHEAMNFAGIHDLPLVFLVENNVYAISVHAEFQVAGSIAKRAEGYGFPGHEVD
                     GNDVLAVYEVAREAFHRARAGEGPTLIEAKTYRMTAHSSDDDDRRYREREEIEEWRQK
                     DPIVRFEKYLMENDVLDESGRDEISERIKAEVAEASKYAEDAPFADPEDVLSGVYAEA
                     "
     gene            1854..2840
                     /locus_tag="VFQ10_08865"
     CDS             1854..2840
                     /locus_tag="VFQ10_08865"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET9965492.1"
                     /translation="MATKNLLQAIHEGLAEEMRSDESVMVLGEDVGRAGGVFRVTQGL
                     QEEFGTARVMDTPLAESLIVGSAIGLSVNGMRPVAEIQFADFIPPAFDQIVNEAAKFH
                     YRSNGDWNCPLTIRAPYGVVPGGALYHSQSIEAWFCNTPGLKVVAPTFPADAKAMIKS
                     AIRDPNPVLFLEHKRTYRLIKEEVPDEDHQVPLGEARVHRQGTEITVVAYGLMLYMAL
                     EVAEKLSEEHGIETEVVEPRTLYPLDREKILDSAKKTGKVLVISEANLTGSVSGEIAA
                     IVAREAFEWLDGPVMRLGAPDVPAAAFARPMMDRFVPSAEKIEEAMLELVRY"
     gene            2851..4191
                     /locus_tag="VFQ10_08870"
     CDS             2851..4191
                     /locus_tag="VFQ10_08870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET9965493.1"
                     /translation="MAKPVTMPQLGESVTEGTITRWLKSEGDEVEIDEPLAEVDTDKV
                     NAELPSPVAGTIQKFLVSEGSTVDVGTEIALVADDGDSPAAEAPPQENVAADSPTEEF
                     PASGTEAQPAAQDTERQRAIARADSDGKGRGEIEDAETLRLTRSSPVVRRIAEEHGVE
                     IAEIPGTGTGGRVTRKDIEGYIEEQEEQYAPPGLQEEREVFAEPEPEIERVPVYEGDR
                     VEDLSGIRRAIANRMSASKREAPHAWTMVEVDVSGLAALREREKAAFAEREGINLTYL
                     PFIVRTVVESLREHPVLNSVWDEDRIILRKRINVGVAVDLEDALIVPVITDADELNLV
                     GLARKIDDLVRRARDRQLGTEDISGGTFTVNNPGALGSVVSTPIINHPQAAILSAEAI
                     VKRPVVIDDAIAVRSMMNVEVSFDHRVLDGGAALKFLNSVKSRLEAYGPESDIG"
     gene            4286..5539
                     /locus_tag="VFQ10_08875"
     CDS             4286..5539
                     /locus_tag="VFQ10_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET9965494.1"
                     /translation="MDVNIKFQRVIEDVLNRIDREELVRLARELVRIPSVYRPEEENG
                     DETRVARFVADYLEQAGFSVRTEEVTPGRPNVWAVWEGDRPGKTLLFEAHTDVVTEGS
                     AEDWDHPPFGAKRDGGRIYGRGACDTKGNLAAAALAVRAVRDSGVTYPGSLILCHPVD
                     EEGMMAGIKAFIEGGHADGVDAAVICEPEENQLCIKQKGALRVEVTLRGTMAHGAMPQ
                     SGVNPVTRAARFVVAVEELEREEIERHAEDSFLGYPSLTPTILMGPSLGEPQINVIPA
                     SAYVALDIRTVPAQSQRDLVERLEEIIARLASEDPDFDATLEVIEERPPTETLKDEPL
                     VRAMARAYDHLTGEEPIYNGVPGATDGTFLHAWAGIPIVTTGAGDREIPHHKDEWVDE
                     GELLTTCKLYAATAMYYAYGEESHV"
     gene            5532..6314
                     /locus_tag="VFQ10_08880"
     CDS             5532..6314
                     /locus_tag="VFQ10_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9965495.1"
                     /translation="MFDYRSMFDLSGKTALVVGAGSGIGEASAQGLAAFGARVVCADV
                     SKEAADDTASEITSEGGKADAIELDITDPDSVRMAVESVGIPDVLVSTPSINVRKPVL
                     EITDDEFDQIVELNLKGTFRLIRDFGRGMVERGSGSIIAFSSIRGQVVEPGQGIYAAT
                     KAGTVQMLRALASEVGPRGVRVNAIAPGIVETPLTAQIKDSPDWYDSYAAKSILGRWA
                     MPHEMVGMVLYLASEASSYVTGAFMLVDGGWTAADGRFTPPL"
     gene            6563..7165
                     /locus_tag="VFQ10_08885"
     CDS             6563..7165
                     /locus_tag="VFQ10_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET9965496.1"
                     /translation="MAMGSGSEGRGSIFRFDTRQIVVAGIIGGIALFLGFTRLGFIPV
                     PIPLIGNATIMHIPAIVGGALEGPIVGLLAGAIFGIFSFLQADNPVFLNPVVAIVPRL
                     LIGVVAWAVFVGLRRWSVDLAAVVAGLLGSFANTVGVVGLAILFGLLPVAIIPAILPQ
                     AIAEAVLAAVVTVVIVRGVMLFRSGKTTAPENESDEERRY"
     gene            7187..8032
                     /locus_tag="VFQ10_08890"
     CDS             7187..8032
                     /locus_tag="VFQ10_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9965497.1"
                     /translation="MIELDNVSFTYRGGETDAVPALLNLDLEIESGQFVAIIGQNGSG
                     KSTLVKLLTAVLYPTEGNIRIDGVPVDKEHRWDVRRKVSVAFQDPDDQLIMNRVIDDV
                     AFGPENLGLAREEIEERVHSSVRMLGLEEIGGRLIEDLSPGQKQRVAIAGALAMRPLF
                     LILDEPTSLLPVPVAMRLISTVRDLGLRENMGVLHVTHSMYEAVLFERVVVMDAGRVI
                     IDGPPAKVFRDVERLREVGLDVPLAASLAHRLRARGIPIEEDILNTADLRASLSAAVD
                     TSPSG"
     gene            8032..8907
                     /locus_tag="VFQ10_08895"
     CDS             8032..8907
                     /locus_tag="VFQ10_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9965498.1"
                     /translation="MQPHGTPPILELKGFAHTYLEDTPKANEAVRDVSFRIQPGERVG
                     IVGATQSGKSTVVDSFAHLLRLTPGQVFYRGEDVVAPGYNRGRLRREIGIVFQQPESQ
                     IIEDVVGADVAFGPTAVGLSASETRERVEESLNTLGLPYADYRLRYVHALSGGQRRRV
                     AIAGVLAMHTPVLVLDEPMAGLDPRGRKELLELLIKLQRDRELTLIVCSASLTDASLL
                     CERFIVLERGTVVMDGPVREVLSQADRLAELDITLPEPVVGAREMQKLFPDFPTDLLT
                     EDELESALLERLGSA"
     gene            8907..9719
                     /locus_tag="VFQ10_08900"
     CDS             8907..9719
                     /locus_tag="VFQ10_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET9965499.1"
                     /translation="MLELQRNVIFGQFVETDSFVHRMDARVKIAATSVLIVASFLIDS
                     FLGFAVVLPLLVVIGVVSKIPLGYILRGSRIFIAFLVFILAFQVLFYPGDVPESSYLW
                     RWGILSVSTEGLYTAGIIALRVILLYYVTTMLMLTTSLVDLTNGLELIFGPLQRLRVP
                     VNELVLVFVIAIKFVPIFIEEMERLARAQTARGVPFDEGGTLTRARRLGRLLVPIFIS
                     GFARADTLTIAMNTRSYRGGRYRTKLRQMRAKSSDWLVLVLVIVWIVIAWMV"
     gene            complement(9971..>10329)
                     /locus_tag="VFQ10_08905"
     CDS             complement(9971..>10329)
                     /locus_tag="VFQ10_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine dehydrogenase"
                     /protein_id="HET9965500.1"
                     /translation="LRCRIVAGSANNVLAESGHGEDLSERGILYAPDYVINAGGLINV
                     ADELEGYNERRATARVMRIEDRIRRIIAISKRDGVPTNVAADTMALERIAAISSMERL
                     HTGHPYGQLSRRRETI"
BASE COUNT         2047 a         3143 c         3296 g         1843 t
ORIGIN      
        1 aacgcttcat ggtcaacgtc aacatctacg aaccccagga cgagcaggac atggagctcc
       61 accgcataga ccccgaggtc tcagaaagtc aggtcgaacg cctcaaagcc ctcaaagaac
      121 gccgcgatga cgacagggtc aaacagtgcc tcgaagagtt gaaggaggtt gccagaggtg
      181 aaggcaacac catgtacccc atcctggagg ccgtaagggc ctacgctacc gtgcaggaga
      241 tctccgacgc cctcaaggac gttttcggcg actaccgcga gacgccggtg atctagagct
      301 tctcagcccg ccgcaaggcc tgagcgtgct gaaatgctgc atcgctgcta tataatccat
      361 ttgatactca cgtgtcagga tgttggggtc ttctggaagg agtcgcgtca tggaagctag
      421 ggtggtggat cttcggatcc ttcccacgga ggcgaaggag gtagttcgca tctacaggga
      481 ctccgtactg ccggccgcaa ggcgtcagca gggattcaag ggcgccctgc tactgacgga
      541 tgacgacact ggggtaggga tctctatctc gctctgggag accgaggagg acagggagga
      601 aggagaggca aacggtttct acaagcagca gatcaggaag ttctcggatc tcctgaccga
      661 gacaccggtc cgcaagcact acaacgtagg cgttctccac tacgacgcca gtgttttagc
      721 gtagcccacg ggtataggcc aacgtgcaca gagtggacgt ctgcggtctg ctagaatttg
      781 ggtgacggag gagtaatttc cgctcctcgc gtggggacct ttgagcagag gaggaggtct
      841 atggctatcc gagagaccag tgctggccac gaggcgctgg ggctggacgc ggacgacctg
      901 cgcaggatgc actactacat gcttctcgcg cggcgggtag atgagaggtc ctggaccctc
      961 aatcgccagg gcaaggctgc gttcgttatc tcctgccagg gtcaggaggc cgcccaggta
     1021 ggcgccgcct ataacctgcg tcccggcaac gatttcctgt acccctacta ccgcgactcg
     1081 gggatgaccc tactcctcgg acagacgccc cgcgaccagt tccttagcct cctgggcaag
     1141 aaggaagacc cgaatagcgg cggacgccag atgcccggcc actacagcag ccgcaaactg
     1201 cacatcgtga catcttctgc cccggtcggg atccaatacc cgcaggccgc aggcacggcc
     1261 ctcgcgtaca agattcgcgg cgaggacggc gtagtgttgg tgtgcggcgg cgaggcgtcg
     1321 actagcggcg gagactggca cgaggcgatg aactttgccg gtatccacga cctgcccttg
     1381 gtcttcctcg tcgagaacaa cgtctacgcc atctccgtac acgccgagtt ccaggtcgca
     1441 ggctcgatcg cgaagcgggc cgagggctac ggcttccccg gccatgaggt agatggtaac
     1501 gatgtgcttg ccgtctacga ggtggcaaga gaggctttcc atcgagcccg cgctggcgag
     1561 ggtccgacgc tcatcgaagc gaagacctac cgcatgacag cccactcttc agacgacgac
     1621 gaccgtagat accgcgagcg cgaggagata gaggagtggc gccagaaaga ccccatcgtt
     1681 cgcttcgaga agtacctgat ggagaacgac gtcctggacg agtcggggag ggatgagatc
     1741 tcggagcgta taaaggctga ggtcgcagag gcgagcaaat acgccgagga cgccccgttc
     1801 gccgatcccg aagacgtact ctccggcgtt tacgcggagg cttaaaggta cctatggcga
     1861 cgaagaattt gctacaggcc atccacgagg ggctcgccga ggagatgcgc tcggacgagt
     1921 cggtaatggt gctcggcgag gatgtaggca gggccggcgg tgttttcagg gttacgcaag
     1981 ggttacagga ggagttcggt actgcgaggg tgatggacac gccgctggct gagagcctga
     2041 tcgtgggctc ggccataggc ctgtcggtta acgggatgcg ccccgtggcc gagatacagt
     2101 tcgcggattt tatcccgccg gcgttcgacc agatcgtcaa cgaggcggcc aagttccact
     2161 accgctcgaa cggtgactgg aattgccccc taaccatccg ggctccctac ggtgtcgtgc
     2221 ccggcggcgc cctctaccac tcccagagca tcgaagcctg gttctgcaat acaccaggcc
     2281 tcaaggtggt cgcgccgacg ttccccgccg atgccaaggc catgatcaag agcgccattc
     2341 gagatcccaa ccccgtcctc ttcctcgaac acaaacgtac ctaccgcctc ataaaagagg
     2401 aggttcccga cgaagatcac caggtccccc tcggcgaggc ccgcgtccac cgccagggca
     2461 cggagatcac ggtcgtcgcc tacggactca tgctctacat ggccctggaa gtagccgaga
     2521 agctctccga agagcacggt atagagacag aggtcgtaga gcctagaaca ctttatccgc
     2581 tggacaggga gaagatcctg gactcggcga agaagaccgg caaggtactg gtgatctccg
     2641 aggctaacct gacgggatcg gtttcgggag agatagcggc catcgtggcg cgcgaggcgt
     2701 tcgagtggct cgatgggccc gtgatgcgcc tcggggcgcc cgacgtgccc gcggcggctt
     2761 tcgcccgccc catgatggac cgcttcgtgc catcggcgga gaagatagaa gaagccatgc
     2821 tcgagctggt gcgctactag aggaggggcc gtggctaaac cggtaacgat gccgcaactc
     2881 ggtgagagcg tgacagaggg cacaataacc cgctggctca agtcagaagg cgacgaggtg
     2941 gagatagatg agcccctcgc cgaggtggac acggacaagg tcaacgccga gttaccctcg
     3001 cccgtggcag gcacgatcca gaagttcctg gtctccgaag gctcgacggt cgacgtgggg
     3061 accgagatcg cactcgtcgc cgacgacggt gattcccccg ccgctgaagc cccgccacag
     3121 gagaacgtcg ctgcggactc tcccacggaa gagttcccgg cctcgggcac ggaagcccag
     3181 cctgcagcac aggataccga gagacagcga gccatcgccc gcgcagacag cgatggcaaa
     3241 gggcgcggcg agatagagga cgccgagaca ctgcgcctca cccgctcctc accggtcgtc
     3301 cggcggatcg ccgaggagca cggcgtggag atagcggaga tcccaggcac gggcacggga
     3361 ggacgcgtaa ccaggaaaga catcgaaggc tacatagaag aacaggagga gcagtacgcg
     3421 ccgcctggac tacaggaaga gcgggaagtc ttcgcggaac ccgagccgga gatcgagcgc
     3481 gtgcccgtct acgagggcga ccgcgtcgag gacctgagtg gcatcaggag ggccatagcc
     3541 aaccgcatgt cagcgagcaa gcgcgaggca ccgcacgcct ggaccatggt cgaggtggac
     3601 gtgagcgggc tcgccgcgct gcgcgagcgg gagaaggccg ccttcgccga gcgcgaaggt
     3661 ataaacttaa cctacctgcc gttcatcgta aggacagtcg tcgagagcct cagggagcat
     3721 cctgttctca actcggtctg ggacgaggac cgcataatcc tcagaaagcg cataaacgtc
     3781 ggcgtagctg tcgacctcga agacgccctc atcgtgcccg tcatcaccga cgctgacgag
     3841 ttgaacctcg tcgggctcgc ccgcaagatc gacgacctcg tgcggcgcgc ccgcgaccgc
     3901 cagctcggta ccgaagacat ctcgggcggc accttcaccg tcaacaaccc cggcgccctc
     3961 ggtagtgtgg tctcgacgcc cataatcaat cacccgcagg cggcgatact atccgcggaa
     4021 gccatcgtca aacgtcccgt cgtgatagac gacgccatcg ccgtacgcag catgatgaac
     4081 gtggaggtct ccttcgacca ccgcgtcctc gacggcggcg ccgcgctgaa attcctgaac
     4141 tctgtcaaga gccgcctcga ggcttacggg ccagagagcg acatcggctg acagatctcc
     4201 ttgcagcagg agaacaggag tgtatggggt tgtcgtggtg catcgaaggt gatgatggat
     4261 cggtgccgac gggcggggcg ccgaaatgga cgtaaacatc aagttccaac gtgtgatcga
     4321 agacgtactg aataggatcg accgcgagga actcgttcgc ctggcgcgtg aactcgtccg
     4381 tatcccgagc gtctaccgtc cggaagaaga gaatggcgac gagacgcgcg tcgcccggtt
     4441 cgtggctgat tacctggaac aggccggctt ctcggtgagg accgaagaag tcacgccggg
     4501 ccgtccgaac gtgtgggcgg tgtgggaagg cgaccgtcct gggaagaccc tgctcttcga
     4561 ggcgcacacg gacgtggtga cggagggcag cgcggaggac tgggaccacc cgcctttcgg
     4621 ggccaagaga gatggaggcc gtatctacgg gcgtggggcg tgcgacacca aggggaacct
     4681 ggcggcggcc gcgctggccg tgcgtgcagt aagggactcc ggtgtcactt accctggcag
     4741 cctgatactc tgtcatcctg tggacgagga ggggatgatg gccgggatca aagcgttcat
     4801 cgagggaggc cacgccgatg gggtggacgc cgctgttatc tgcgagcccg aggaaaacca
     4861 gctctgcata aagcagaaag gcgcgctcag ggtggaggtt actctgcgtg gaacaatggc
     4921 gcacggggca atgcctcaga gcggcgtcaa cccggttacg agggccgcga ggttcgtcgt
     4981 cgcggtcgag gaactggaga gagaggagat cgagcgccac gccgaagatt cgttcctcgg
     5041 gtatccgagc cttacgccga ccattttgat gggcccaagc ctcggtgagc cccagatcaa
     5101 cgtcatcccg gcgagcgcct acgtcgccct ggacatccgt acggtgccgg cgcagtctca
     5161 gagagatctc gtagagcgac tggaagagat catcgcgagg ttggcctcag aagaccccga
     5221 cttcgacgcg acgctggagg ttatcgaaga gcgcccgcca accgagaccc tgaaagacga
     5281 gcctctcgtc agggcgatgg caagggccta cgatcatctg accggcgagg agccgattta
     5341 caatggggtc cctggcgcga cagacggcac tttcctgcat gcttgggccg gcatccctat
     5401 cgtcacgacg ggagccgggg accgtgagat cccacaccac aaagacgagt gggtcgatga
     5461 aggggaactc ctcaccacct gcaagctcta cgccgctacg gcaatgtact acgcctacgg
     5521 agaggaatcc catgtttgat taccgctcga tgttcgacct ttctggcaag accgccctcg
     5581 tggtcggggc cggcagcggc atcggcgagg ccagcgcaca aggactcgcg gccttcggcg
     5641 cgcgcgtcgt gtgcgccgac gtgagtaagg aggctgcgga cgacactgcc agcgagataa
     5701 cgtcggaggg aggaaaggcc gatgctatcg agcttgacat tacggacccg gacagcgtaa
     5761 ggatggccgt cgagtctgtg ggaattcctg atgtcctggt gagcacgccg tccatcaacg
     5821 tcaggaagcc tgtgctcgag ataacggacg atgagttcga ccagatcgtc gagctcaact
     5881 tgaagggtac tttccgcctg atcagggact tcggccgcgg catggtcgag cgcggctcag
     5941 gcagcatcat agccttctcg agcatcaggg gacaggtcgt cgaaccggga caggggatct
     6001 acgcggcgac gaaggcaggg acggtgcaga tgctcagggc ccttgcatcc gaagtaggac
     6061 cccgcggtgt gagggtcaac gccatcgctc caggcatagt cgagacgcct ttgacggccc
     6121 agatcaaaga ctcccctgac tggtacgatt cctacgcagc caagagcatc ctcggccgct
     6181 gggccatgcc gcacgagatg gtcgggatgg tcctctacct cgcctcagaa gccagctcct
     6241 acgtcacggg cgccttcatg ctggtcgacg gtggctggac cgccgcagac ggacgcttca
     6301 cgccgccact ctaagtgttg atacggtatc cgcatgatca cttccgcggg gtacaatctc
     6361 ctgcgcgagc gcaagtctta ctcccttgcc ccggcatcac catccttcag tcccgcgcag
     6421 gcgggaatcc acttcaccag aggatctccg cacctccaac cagaggcgat ccaccggatt
     6481 acgtgtgaca cagctgcgcg cagataccgc ggtccgtata tagtagtgct gacctgatag
     6541 taaatagcag aaggagaagg tcatggcgat gggttcgggg agcgaagggc ggggctccat
     6601 attccgtttc gacaccaggc agatagttgt ggccggcata ataggcggca tcgcgctgtt
     6661 tctcgggttc acgcgcctgg gctttatacc ggtaccgata ccgctcatag gtaacgctac
     6721 gataatgcac ataccggcga tcgtcggtgg ggcgctggaa ggacccatcg ttgggctttt
     6781 ggccggtgcg atcttcggga tattctcctt tctacaggcg gacaacccgg tattcctcaa
     6841 ccccgttgtc gccattgtgc cgcgcctgct catcggggtg gtggcgtggg ccgtcttcgt
     6901 ggggctgcgg cgctggagcg tggacctcgc cgcggtcgtg gccggattgt tggggtcctt
     6961 cgccaatacg gtaggggtgg tcggtctcgc catactcttc ggattgctgc ccgttgccat
     7021 aattccggcc atcctgccgc aagccatagc cgaggccgtg ctggccgcgg tcgtcacggt
     7081 cgtcatcgtc aggggtgtta tgctcttccg cagtggcaag acgaccgccc cggagaacga
     7141 gtcggacgaa gaacgccgct attaatcaag gcgcggcgcg agcgcgatga tcgagctaga
     7201 caacgtctct ttcacctatc gtggtggcga gaccgatgcg gttcccgcgc ttctgaacct
     7261 cgacctggag atagaatcgg ggcagttcgt cgcgatcatc ggccaaaacg gctcggggaa
     7321 gagcacgctc gtcaagttgc ttaccgccgt cctctacccg acggagggga atatccgtat
     7381 agacggcgta cccgtggata aggagcaccg gtgggacgtg cggcgcaagg tatccgtcgc
     7441 cttccaggac cccgacgacc agctaataat gaaccgtgta atagatgacg tggctttcgg
     7501 accggagaac ctggggttgg cacgggagga gatcgaggaa agggtgcact catctgtgcg
     7561 tatgctcggc ctcgaggaga tcggcggtcg cctgatcgag gacctctcac cggggcagaa
     7621 gcagagggtg gccatagcgg gcgccctggc gatgcggccc ctattcctga tcctggacga
     7681 accgacctcg cttctgcctg tgcctgtcgc gatgcgcctg atctctaccg taagagacct
     7741 gggcctccgc gagaacatgg gcgttctgca cgtcacccac tccatgtacg aggccgtcct
     7801 cttcgagagg gtcgtggtca tggacgctgg gcgtgtgatc attgacggcc cccccgccaa
     7861 ggtcttccgg gacgtcgaga gactgcggga ggtcggcctt gacgtgccgc tagccgcgag
     7921 cctcgcccac cgcctgcgcg cccgcggcat cccgatcgaa gaagatatcc tgaacacggc
     7981 cgacctgcgc gcctccctct ctgccgccgt ggatacttcg ccttcaggct gatgcagccc
     8041 cacggaacgc cccccatcct ggagttgaag ggctttgccc acacctacct ggaagacacc
     8101 ccgaaggcga acgaggcggt aagggacgta tccttccgga tccaacccgg cgagcgcgtc
     8161 gggatagtcg gcgccaccca atcgggcaag tccaccgtcg ttgactcttt cgcgcacctg
     8221 ctgcgcctaa cgcctgggca ggtcttctac agaggcgagg acgtggtcgc tcccggctac
     8281 aacagaggca ggctgcggcg cgagatcggg atcgtgttcc agcagccgga gtcacagatc
     8341 atagaagacg ttgtcggcgc ggatgtcgcc ttcggaccga ccgccgtagg cctctccgcc
     8401 agcgagaccc gcgagcgcgt cgaggagagc ctgaatacac tggggcttcc ttatgcggat
     8461 taccgcttac gttacgtcca cgcgctctcc ggcggccagc gcaggcgggt cgccatagcc
     8521 ggggtgctcg ccatgcacac gcccgtgttg gtcctcgacg agccgatggc agggcttgac
     8581 ccgcgcggcc gcaaggagct tctggaactt ctcatcaagc tgcagcgtga ccgcgagctg
     8641 accctgatcg tctgctctgc aagcctcacc gacgccagcc tgctatgcga gcgctttatc
     8701 gtcctggaga ggggcacggt cgtcatggac ggacccgtgc gcgaggtatt gagccaggcc
     8761 gataggctcg cagagctgga cataacccta cccgagccag tcgtcggcgc ccgcgagatg
     8821 cagaagctct tccctgactt cccgaccgac ctgctgaccg aggacgagct cgaatctgcg
     8881 ctgctcgaga gactgggatc tgcgtagtgc tcgagctgca gcgtaacgtc atcttcgggc
     8941 agttcgtcga gacggactct ttcgttcatc gcatggacgc ccgcgtcaag atcgccgcga
     9001 cgtccgtgct catcgtcgcc tcgttcctca tagacagctt cctcggcttc gccgtcgtgt
     9061 tgccactgct cgtcgtcatt ggcgtagtct ccaagatacc cctcgggtac attctgcggg
     9121 gctcgcgaat cttcattgcg ttcctagtct tcattctcgc cttccaggta ctcttctacc
     9181 ctggagacgt gcctgaatcg agctacctgt ggcggtgggg gattctctcg gtctcgaccg
     9241 agggtctcta cactgcgggc atcatcgcgc ttcgcgtcat cctcttgtac tacgtcacga
     9301 ccatgcttat gctcacgacc tcgctcgtag acctgacgaa cgggttggaa ctcatcttcg
     9361 gcccgttaca acggctcagg gtacctgtca acgagctggt gctcgtcttc gtgatcgcga
     9421 ttaagttcgt gccgatcttt atcgaggaga tggagcgtct cgcccgcgcc cagacggccc
     9481 gcggcgtccc cttcgacgag ggcggcacac taacccgggc gcgccgcctc ggacgcctac
     9541 tcgtccccat tttcatcagt ggcttcgccc gcgccgacac cctcaccatc gccatgaaca
     9601 cccgcagtta ccgcggcggc cgctaccgca caaagctccg ccagatgcgc gcgaaatctt
     9661 ccgactggct ggtgcttgtg ctggtcatcg tctggatagt catcgcgtgg atggtttaga
     9721 gcgtttcagc acgcgccctg tcgggcgctt gtcagcattt cagcacggcg gctgcgctac
     9781 cttgtcagca cttcagcaag tcgtcccata gcctctgcac gagcgtcgaa gatgaccacc
     9841 gcaaatagta gttcctcgtg cgctgcgagg ccgaagcggg aaaagctaac atgctgacaa
     9901 gctgaggccg caggccgaag cgtgctgaaa tgctgaaatg cggagaccat atagggcgtg
     9961 ctagcacgcc ctatatggtc tccctgcggc ggcttaactg cccgtagggg tgtcctgtat
    10021 ggagacgctc catcgagctt atggcggcta tcctctccag cgccatggtg tctgcggcga
    10081 cgttggttgg gacgccgtcg cgtttggaga tggcgatgat gcggcggatc cggtcctcta
    10141 tccgcatgac gcgcgcggtc gcgcggcgtt cgttgtagcc ctccagctcg tcggcaacat
    10201 tgataaggcc gccggcgttt atgacgtagt caggggcgta gagtatgccg cgttcggaga
    10261 ggtcctcgcc gtggcccgac tcggcgagca cgttgttggc gctccctgcg acgatgcggc
    10321 agcgcaact
//
LOCUS       DASTKM010000214        10047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_415684, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000214 DASTKM010000000
VERSION     DASTKM010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415684"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /gene="era"
                     /locus_tag="VFQ10_08910"
     CDS             <1..735
                     /gene="era"
                     /locus_tag="VFQ10_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET9965501.1"
                     /translation="YQAIFVDTPGSQKPRDTLRARMQEHVVDSLSESDVILLLFDALQ
                     ASEGLGSGDRYVARLVASSGTPAIVSINKTDLLRTRESALPIVEAVSELGVWNEVFQI
                     SAAEGTNVEPLMDSVVDLLPHGPRYFPEGTVTDYPESLVLAEYVREKALNVLREEVPH
                     AVAVEIEDVEIKENVTVVYAVIHVERATQRMIVLGKGGRTIKKIGTEARRDVERLLGT
                     RIYLDLNVKVSQGWRNDRQFLERLGL"
     gene            781..1482
                     /gene="deoC"
                     /locus_tag="VFQ10_08915"
     CDS             781..1482
                     /gene="deoC"
                     /locus_tag="VFQ10_08915"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET9965502.1"
                     /translation="MRVREFAKTVDHTLLKPEATEQGIVRLCEQASHFHFAAVCIPPS
                     YVPLAARELRGADVKVATVISFPFGYEPTAVKIAAVREAVTRGASELDVVMNISKFLS
                     GEFGYVADELASLTQEVSAVAINNGLNDVVVKVILETAYLSDKMKRLATQIVAASGAD
                     FVKTSTGFAPVGATPEDVALLREEAPEGLAVKASGGISTLRDALDILNAGASRLGMSA
                     SVAVMEEAEDGGLQE"
     gene            1463..2215
                     /gene="recO"
                     /locus_tag="VFQ10_08920"
     CDS             1463..2215
                     /gene="recO"
                     /locus_tag="VFQ10_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET9965503.1"
                     /translation="MAVYKSKGIVLRSIRYSEADRILDIYTRDAGLISAIAKGIRRTK
                     SRFGARLEPLSCVDFVAYHGGTLDTVTQAETLRSFHGVREDLARFEAAAGMVGSVRAI
                     SGGDEADRRVFNLLYNALDTLETCDSGFENIEAALGIKLSILAGYGLQLDVCLACGMD
                     LDAASEPLHFAPDHGGVLCQECRPATGVAFPLAPGTLVSLRTLVEQPIRQAPVQTGPK
                     QSVLRVVRSHVLAHAPGNTLIGPRLAGSGRPA"
     gene            2212..3198
                     /locus_tag="VFQ10_08925"
     CDS             2212..3198
                     /locus_tag="VFQ10_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HET9965504.1"
                     /translation="MRFQGPSVGLCHDPSVWAVEHSVGRPRPEPPDGVRNDFQRDRDR
                     IIHAKAFRRLMHKTQVFISPDGDHFRTRLTHTLEMMQISRTIARALGLNEDLTEAISL
                     GHDLGHTPFGHTGEEALSRVLEAHGRTFRHNEHSLRVVDHLENQGDGLNLTFEVRDGI
                     RNHTGKGYPRTREGEIVRVADRIAYVNHDVDDALRAGIISSHDLPPSPISVLGVQMSA
                     RINTLVRDMISNSEMKGEISLSDPVYGALMELRAWLFRHVYNNKLGSRENQKAARVVT
                     DLFVYYLEHPEERVGSDPDPLTETIDFVAGMTDRFALATYRRLFLPRGEIFA"
     gene            complement(3408..4499)
                     /locus_tag="VFQ10_08930"
     CDS             complement(3408..4499)
                     /locus_tag="VFQ10_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965505.1"
                     /translation="MNRLYILLTVMVVMVGVLFTGQEALAEVLIGTDSEDTLVGTEGA
                     DRLDGRAGDDRLDGLGGDDTIEGGDGNDEIFPGEGDDDVFAGAGDDLIFARDTNGVDF
                     IDCGDGFDEVETIHRDDKTKRNCERALGPSAGTITGTTGTTGTTTGTTTGTTTGTTGT
                     TGTACQNPQEVLTVGPTTENTRTPFTTTGNVFRVSYDVNFNDRRAFNSADIDIIEDRF
                     GLVDFVNLSEDANNSFIVTEGAGTYDLVVRIDPPNGATYTVTVEDCRGTTTGTTDTTE
                     TTGATTATTGATTGRTTGASTGEASTTGAATTGGDSTSRRDGVIRDTIPQGQQLPNTG
                     GPTGLVPAAALLALLINGAAIGLLFVLRR"
     gene            complement(4753..7545)
                     /gene="ppc"
                     /locus_tag="VFQ10_08935"
     CDS             complement(4753..7545)
                     /gene="ppc"
                     /locus_tag="VFQ10_08935"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET9965506.1"
                     /translation="MPRAGYADLPPGIGFEPKDEPLRRDINLLGRVLGRILIEQEGED
                     LFKTEEEVRLLCKRLRFDYDPALDEQLRRRIDAMSVEELRRIVRAFSVYFQLVNIAER
                     YHRVRRRRQYESSPDNPPQRASVASALARLKSEGLGKEALRSVLDEMSVGLVLTAHPT
                     EAMRRSVRRKHVRIGEMLEAMESPDLTWKEQRRLEEDLAEEITVLWQTEELRVRRPEV
                     NQEIERTLLFFENPLISATLEAYREFEDELSRQFLEDAPRLGRVLEYGSWVGGDQDGN
                     PFVEPEMITTALGLHRELIIERHLASVVWLADHMSQSAGMIPVSEELRRSIGRDEGLM
                     PGLVGRYRGVDPNEVYRRKLLFMSERLRLALWQPGAPAAYSGVTEFLDDLFTIRDSLL
                     QNGGVRVAEGGLRNLIRQAEVFGFHLAKLDVRQESSTVVRAVADLLSADTGEDLLAMD
                     EAARAALLRRLLADPGLSLPEPEDVSEETHRVLETFGRIRRAKEEHHGTPVETFILSM
                     ARNASDVLSVQLLARRAGLLEVDKGGGCTANYLRVTPLFETVDDLGRAPEVLGHLLED
                     PFYRSSLTQSNDLQEVMLGYSDSGKDAGYVTSNWTLYKAQGLLSSVARKYGVNLRLFH
                     GRGGSAGRGGGPSYQAIMAQPPHTLQGRIRITEQGEVISFKYSMRGLARRNLDTVLAA
                     VLEASADETPDEPEPLWVEVMAGLSAKAREAYRNLVYGDQDFLTFFSEASPIGELSLL
                     NMGSRPVRRVQNPAVDNLRAIPWVFAWTQNRFLLPSWYGAGTALGGYASTEQGLVVLR
                     EMYLGWPFFRTLVDFMQMTLAKSDMRIAEHYTSLVSDPDIRSRLWTRISEEHADCVRA
                     ILLITENENLLDDSPVLQRSIRLRNPYVDPLSYVQVALLRRLRALPESSQEREGVLNT
                     LLLSISGVSSGMLNTG"
     gene            7644..9443
                     /gene="dnaG"
                     /locus_tag="VFQ10_08940"
     CDS             7644..9443
                     /gene="dnaG"
                     /locus_tag="VFQ10_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET9965507.1"
                     /translation="MRISQRSIEEVREAAGIVEVASEFTALRRQGARFTGLCPYPDHQ
                     EKTPSFSVTPDQGFYYCFGCSRGGDAIKLVMDLKSLDFAEAVSYLADRSGVDLHFEGG
                     GDPGATRERNLRRRAIHRALAAATAYYHKYLLRSETPEAERARRYLNDRHIELSTIEE
                     FRLGYAPPRGVSGFVAAAGRLDLDRSILDAAGLLSPRGGERFAGRITFPISDRRGRIV
                     GFGARTLGEAQPKYLNSPETEIFNKRDLLYGFPQVSEAIRRERSALVVEGYTDVLMLY
                     QSGIQNAVATLGTATTPGHLRMLSSYTDRIHVIFDPDAAGERAIERAAATAADLKLDL
                     RVLRLPEDPADWLLEHSPGEFAERLNDSVPVLEYGIRRIVARTRGAGATERSRAVPEV
                     KSMIQEIRDPVLYREAVRIASEALGVEPTIFEAPRSDSSAAGAYRSIRKESGHYLREA
                     ERLREVERTHEAEREILALCIVRPDLSVELFQEGIRVPQLQENVHLEESDFVDEAHAN
                     IFAILKEYVGKDIGSVTSDPRARPFLDRLGELSQEGERRPSSEGAMQAAWLRLRIVSR
                     KRLMRETSDYDVKDSLRAEVEALRQGVTFVESS"
     gene            9483..9555
                     /locus_tag="VFQ10_08945"
     tRNA            9483..9555
                     /locus_tag="VFQ10_08945"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9516..9518,aa:Asn,seq:gtt)
     gene            9585..9658
                     /locus_tag="VFQ10_08950"
     tRNA            9585..9658
                     /locus_tag="VFQ10_08950"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9619..9621,aa:Ile,seq:cat)
BASE COUNT         1959 a         3026 c         3028 g         2034 t
ORIGIN      
        1 taccaggcaa tcttcgtgga taccccgggc tcccagaagc cccgcgacac cctgcgagcc
       61 aggatgcagg aacatgtcgt ggacagtctc tctgagtcgg atgtaatcct gttgctcttc
      121 gatgcgctgc aggcatcgga aggacttggg agcggcgacc gttacgtcgc ccgcctcgtc
      181 gccagctcgg gtaccccggc catcgtctcg atcaacaaga ccgacctctt gcgtacccgt
      241 gaaagtgcct tgcccatagt agaggcggtc tcggagctcg gggtttggaa cgaggtgttc
      301 cagatcagcg cggccgaagg caccaacgta gaaccactga tggattccgt tgtcgatctc
      361 ctgccacacg gtccccgcta tttcccggag ggaacagtga cggactaccc tgagtctctg
      421 gtcctcgccg agtacgtgcg ggagaaggcg ctgaacgtgc tgagggaaga ggtgccgcat
      481 gccgttgccg tcgagatcga agacgtagag atcaaagaga acgtgacggt tgtctacgcc
      541 gtcatccacg tcgaacgtgc tactcagcgt atgatagtac tgggcaaagg cggcaggacc
      601 attaagaaga tcggcaccga ggcccgccga gatgtcgagc gtctcctcgg cacccgtatt
      661 tacctcgacc tgaatgtaaa ggttagccag ggatggagaa acgacaggca gtttctggag
      721 cggttgggtt tataatcacc ctggtctatt gtgtcgagag aggagcgtag agttttggcg
      781 atgagagtac gcgagttcgc gaagacggtg gaccatactc tcttgaagcc cgaggccaca
      841 gagcagggga tcgtacgcct ctgcgagcag gcttcccact ttcatttcgc cgcggtgtgc
      901 atcccgcctt catacgtccc tcttgccgcc agggagttgc gtggtgccga cgtaaaggtg
      961 gcgacggtga tctcgttccc attcgggtac gagcccactg cggtgaagat tgcggccgtc
     1021 cgcgaggcgg ttaccagggg tgcttcggag ctcgatgtag tcatgaacat ctcgaagttc
     1081 ctctccggag agttcggcta cgtcgctgac gagctcgcaa gcctcaccca ggaggtaagc
     1141 gcggtagcta tcaacaatgg gctgaacgac gtggtcgtca aggtcattct cgagaccgcg
     1201 tacctctcgg acaagatgaa gcggctggcg acccagatcg tggccgcgag cggcgcggat
     1261 ttcgtgaaga cctcgacggg cttcgctcct gtcggggcaa cacctgagga cgtggccctg
     1321 ctcagggaag aggctcccga gggccttgca gtaaaggcgt caggcggcat cagcaccctc
     1381 agggacgccc tcgacatact caacgcaggt gcttcgcggc tcggcatgag cgctagcgtc
     1441 gccgtgatgg aggaggccga ggatggcggt ctacaagagt aaggggatcg tgctacgttc
     1501 gatccgctac tcggaggcgg acaggatttt ggacatctac accagggacg ctgggctgat
     1561 ctcggcaatc gcgaagggta tcaggcgtac aaaatcccgc ttcggagccc gcctcgaacc
     1621 actctcctgc gtcgacttcg tcgcctacca cggcgggacg ctcgacaccg tgacccaggc
     1681 ggagacgctg cgcagcttcc acggtgtcag ggaggatctc gcccgcttcg aggccgccgc
     1741 aggtatggtc gggagcgtgc gcgccatctc gggtggcgac gaggcggacc gcagggtctt
     1801 caacctgctc tacaacgccc tagacacgct ggaaacgtgc gattctgggt tcgagaacat
     1861 cgaggcggcc ctcggcataa agctctccat cctcgccggc tacggtctgc aactcgatgt
     1921 ctgcctggcc tgtgggatgg acctcgacgc ggcttcggag ccgctgcatt ttgccccaga
     1981 ccacggggga gtcctgtgcc aggagtgccg ccctgcgacc ggtgtcgcgt tcccgcttgc
     2041 cccgggtacg ctggtgagcc tgaggaccct ggtagaacag cccatccgtc aagcgccagt
     2101 acaaactgga cctaaacaga gcgtcctcag ggtagtgcgc tcccacgtcc tcgcccacgc
     2161 tccggggaat acgctgatcg gtccccgact agccggctcc ggacgcccgg cgtgagattc
     2221 caaggaccca gtgtcggcct ttgccacgac ccttctgtat gggccgtaga gcacagcgta
     2281 ggccgcccca ggcccgagcc tcccgacggt gtccgcaacg acttccagcg cgacagggac
     2341 cgcatcattc acgccaaggc cttccgcagg ctcatgcata agactcaggt tttcatctcc
     2401 cctgatggcg atcacttcag gacacgcctc acgcacaccc tggagatgat gcagatatcc
     2461 cgtaccatag cccgagctct cggcctcaac gaagacttga cggaggcgat ctccctcggc
     2521 cacgatctcg gccacacccc cttcggccat actggagaag aagccctctc ccgcgtcctc
     2581 gaagctcacg gacgcacctt ccgccacaac gaacactccc tgcgcgtagt cgaccatctc
     2641 gagaaccaag gagacggact aaaccttaca ttcgaggtca gagatgggat tcgtaaccat
     2701 acaggaaagg gctatccacg taccagggaa ggtgagatcg tacgcgtcgc tgacaggatc
     2761 gcctacgtca atcacgacgt ggacgacgcc ttgcgggctg ggatcatctc tagccatgac
     2821 ctcccgccga gccctatctc tgttctagga gtgcagatga gcgccaggat caataccctg
     2881 gtaagggata tgatctctaa ctctgaaatg aaaggtgaga tatccttatc ggacccggta
     2941 tacggggcgc tcatggagct cagggcgtgg ctcttccggc acgtgtacaa caacaaactg
     3001 ggctcgaggg agaaccagaa agcagcccgc gtcgttacgg acctcttcgt atactatctg
     3061 gagcacccgg aagagcgggt gggcagcgat cccgacccac ttactgagac catagacttc
     3121 gtggcgggga tgaccgacag attcgccctc gcgacataca ggcgcctctt tctgccccgg
     3181 ggtgagatct tcgcgtaagt gcgagtcctg cggagaccgg catgaatccg aaaggcgttg
     3241 acctatcggc accaagcaag gcatgtgatt ctctggacca ccgactgtgc cgagcacatt
     3301 ctccaactct tcgaggcgaa ttatccgata gacgaccgag ctaggataag gacagaagcg
     3361 cggtaatctg ttcagaatag acctctttcc ctccaaagtg aagagtgcta ccgtcggagc
     3421 acgaacaaca accctatggc cgccccgttg atgagcaacg ccagcagagc cgccgctgga
     3481 acgagcccgg tggggccgcc tgtgttgggt agttgctggc cctgggggat cgtgtcgcga
     3541 atgacgccgt ccctgcgcga ggtgctatcg ccgcctgtgg tggctgctcc agtagtgctc
     3601 gcttctccgg tgctagctcc ggtagtcctc cctgttgtcg cacccgtagt ggcggtagtg
     3661 gcgccggtgg tttccgtagt atccgtcgtt cccgttgtcg taccgcgaca gtcctcgaca
     3721 gtcaccgtgt aggtcgcccc gtttggagga tcgattcgta ccacgaggtc atatgtgcca
     3781 gcgccttcgg tcacaatgaa gctgttattt gcgtcctcac tgaggtttac gaagtccact
     3841 agcccgaaac gatcttcgat gatgtctata tctgcggaat taaatgcccg gcgatcgttg
     3901 aaattaacat cataagatac gcgaaagacg ttaccggtag tcgtaaaggg agtcctggtg
     3961 ttttcggtcg taggcccgac ggttagcacc tcttgaggat tttggcaggc agtgcctgtg
     4021 gtgcccgtcg ttcccgttgt cgtgcctgtg gtggtgcctg tggtggtgcc tgtggtgccc
     4081 gtcgtccccg ttatcgtacc tgcgctaggg ccgagggctc tctcgcagtt tctcttggtc
     4141 ttgtcgtcgc ggtgtatggt ctcgacctcg tcgaagccat ccccacaatc tatgaaatcc
     4201 accccgttgg tgtcgcgggc gaagataagg tcgtcgccgg caccggcgaa tacgtcgtcg
     4261 tcgccctcgc cggggaagat ttcgtcgttg ccgtcgccac cttcgatcgt gtcgtcgccg
     4321 ccgagaccgt cgaggcggtc gtccccggcg cgaccgtcga ggcggtcggc cccttcggtc
     4381 cccacgagcg tatcctcgct gtcggtgccg atgaggacct cagccagggc ttcctgcccg
     4441 gtgaagagca ctccgaccat aactaccatc accgtgagca agatgtatag ccgattcatt
     4501 cgacttcctc ccctaatcta cttggagctt gtcctgctcg ctttctcttt agatctcatc
     4561 aactgacact ggttcaaggc ctctagccca cgatggttgg ccgatcgcct atgtcacagg
     4621 cggaacaata gcagattggg gacttggcgc aacttaacag gacttccgag aactcctttt
     4681 ctcctcatga cagtaagtat actagcggaa ccggtaacag ttgagagaca ccccacgggg
     4741 ctgcggagca actcaacctg tattcagcat cccggaggaa acacctgaga tagagagcag
     4801 taaggtattc aagacacctt cgcgctcctg ggagctttcc gggagtgcgc ggagccgacg
     4861 caggagcgcg acctgaacgt aggagagcgg gtctacatac gggttgcgaa gacgtatgga
     4921 tctctgtagg acgggactgt cgtcgaggag gttctcgttc tccgtgatga ggagtatagc
     4981 ccgcacgcag tcggcgtgtt cctcggagat cctggtccac agacgactgc ggatgtcggg
     5041 gtccgagacc agcgatgtgt agtgctcggc tatacgcatg tcgctctttg ccaaggtcat
     5101 ctgcataaag tcaacgaggg tacggaagaa gggccagcct aagtacatct cgcgcaggac
     5161 caccagtccc tgctctgtgg aggcgtagcc acccagggcg gtccctgcgc cgtaccagga
     5221 cgggagtaga aagcggttct gggtccaggc gaacacccag ggtatggccc tgaggttgtc
     5281 gaccgcgggg ttctgcaccc gccgcaccgg acggctaccc atattgagca gggagagctc
     5341 cccaatagga gatgcctcgg agaagaaagt caggaagtct tggtccccgt agacgaggtt
     5401 gcggtaggct tcgcgcgctt tcgctgagag tcctgccatt acctcgaccc acaggggctc
     5461 tggctcgtcg ggggtctcgt cggcactcgc ctccaggacg gcggcgagca ccgtgtcgag
     5521 gttgcggcga gccaggccgc gcatgctgta cttgaaagag atcacctctc cctgctcggt
     5581 gatccggatc ctgccctgca gcgtatgagg gggctgggcc atgatcgcct ggtagctggg
     5641 ccctcctcct cgtcctgcgc tgcctccccg cccgtggaag agcctgaggt tcacaccgta
     5701 cttgcgcgcg accgaggaga gaaggccttg cgccttgtac agggtccagt tgctcgtgac
     5761 gtaaccggcg tccttacccg agtcgctgta gccgagcatt acctcctgca ggtcgttgct
     5821 ctgggtgagg gaggagcggt agaaggggtc ctctagcaag tgtccgagta cttcgggagc
     5881 ccgcccgagg tcgtccacag tctcgaagag gggcgtcacc ctgagataat tggcggtgca
     5941 cccgccgccc ttgtcgacct cgagcagtcc cgcccggcgg gcgagtagct gtacgctcag
     6001 gacgtcgctc gcgttgcgtg ccatgctgag gatgaacgtc tcgactggcg tgccatggtg
     6061 ttcctccttc gcgcgccgga tgcgcccgaa ggtctcgaga actctgtgtg tctcttccga
     6121 gacgtcttcg ggttcgggaa gcgagaggcc agggtctgcg aggagccttc tgagcagcgc
     6181 ggcccgcgcg gcttcgtcca tggccaggag gtcttcaccc gtgtcggccg agagcagatc
     6241 ggcgacggcc ctgactaccg tggagctctc ttggcggaca tccagcttcg ccaggtggaa
     6301 gccgaagact tcggcctgtc ggattaggtt cctgaggccc ccctccgcga cgcgcactcc
     6361 gccattttgc aggaggctat cgcggatcgt aaacagatcg tcgaggaact cggtcacacc
     6421 agaataagcg gcaggggcgc ctggttgcca gagcgctagc ctcaagcgct ctgacatgaa
     6481 caggagcttg cgccgataga cctcgttcgg gtcgaccccc ctgtaccgcc cgacgaggcc
     6541 gggcattagc ccttcgtccc tcccgatcga gcgtcgcaat tcttcagaga ctgggatcat
     6601 acccgccgac tggctcatgt ggtccgccag ccacactacg gaggcgaggt ggcgctcgat
     6661 tatgagctcg cggtgaagcc cgagcgcggt ggttatcatc tcaggctcga cgaaaggatt
     6721 gccgtcctga tccccgccga cccaagagcc gtattcgagg acacgaccca gcctcggagc
     6781 atcctccaaa aactggcgtg ataactcgtc ctcgaactcc ctgtacgctt cgagcgttgc
     6841 ggagatcaaa gggttctcga agaacagcag tgttcgctca atctcctggt ttacttctgg
     6901 acgacggact ctgagctctt cggtctgcca cagaacggtg atctcctcag ccagatcctc
     6961 ctccagacgc ctttgctcct tccacgtgag atcgggcgac tccatagcct cgagcatctc
     7021 tccgatcctg acgtgcttgc gcctgacgct ccgccgcatg gcctccgtcg gatgggcggt
     7081 caaaacgagg ccgacagaca tctcatcgag tacactccgc aatgcttctt tccccagacc
     7141 ttcgctcttg aggcgtgcga gggcgctcgc cactgaggcg cgttgggggg gattgtccgg
     7201 tgacgactcg tattgccgcc gccgacgaac ccgatggtag cgctcggcga tgttcacaag
     7261 ttgaaagtac acggagaacg cgcgcacgat ccgtcgcagc tcctccacgc tcatggcgtc
     7321 gatccgtcgc cgcagctgtt cgtccaatgc agggtcgtag tcgaagcgca ggcgtttgca
     7381 gaggagcctg acctcttcct cggttttgaa gaggtcctcg ccctcctgct cgatcaggat
     7441 ccggccaagc acccgcccga ggaggttgat gtccctccgc agcggctcat cttttggctc
     7501 gaagccgatc ccgggcggaa ggtcagcgta cccggctctt ggcaccgtcg tagatccgcc
     7561 gcggatattc atgccaggta gagtaacatt acccctggtc aacaccacac tcaatctgta
     7621 caccggtaca agggagaccg ggtttgagga tctcacaacg cagcatagaa gaggtcaggg
     7681 aggcggccgg gatcgtcgag gtggcctccg agttcacggc cctcaggcgc cagggagccc
     7741 gcttcacagg cctctgtccc tacccagacc accaggagaa aaccccttcc ttcagcgtca
     7801 ccccggacca gggcttctac tactgttttg gctgttctcg tggcggggac gccatcaagc
     7861 tggtaatgga tctcaagtct ctagacttcg ccgaagcggt ctcctacctc gccgaccgct
     7921 ccggcgtaga cctgcacttc gaaggcgggg gagaccctgg cgccacgcgg gaacgtaacc
     7981 tccgtagacg cgcgattcac agggccctgg ccgccgccac tgcctactat cacaagtacc
     8041 tcctcagatc cgaaacccca gaagcagaac gggcccgccg ctacttgaat gaccgccata
     8101 tagagctttc tactatagaa gaatttcgtt tggggtacgc gccgccgcgc ggtgtatccg
     8161 gatttgtggc tgctgctggg aggttggatc tcgacaggag tattctcgat gcggccgggc
     8221 ttctttcgcc gcggggtggg gagcgttttg cgggtaggat caccttcccc atctcggacc
     8281 ggcggggaag gatagtcggc ttcggggcca ggacgctcgg tgaagcccag cccaagtatc
     8341 tcaactctcc cgagacggag atcttcaaca agcgggatct gctctacggt ttcccgcaag
     8401 tctccgaagc tatccgtagg gaacgctcgg cgctcgtcgt cgagggctac acggatgtac
     8461 ttatgctcta ccagtcgggc attcagaatg ctgtagcgac gcttggtact gccactaccc
     8521 ctggtcacct gcgcatgctc agctcttaca cggataggat ccacgtcatc ttcgaccccg
     8581 acgcagcagg ggagagggcc attgagcggg cggcggccac agcggccgat ttgaagttgg
     8641 atctccgggt cctacgttta cccgaggacc ctgcggactg gctcttggag cattcgcccg
     8701 gggagtttgc agagaggttg aacgattctg tgcccgtcct cgagtacgga atcaggcgga
     8761 tcgttgcccg taccagggga gcgggtgcta cggaacgctc cagagccgtt cccgaggtga
     8821 agagtatgat ccaggagatc agggacccgg ttctctaccg cgaggccgta cgcatcgcat
     8881 cagaggccct gggcgtagag ccgacgatct tcgaggcacc cagatctgat tcgtccgcgg
     8941 ccggagctta tcggtcaata cgcaaagagt caggtcacta cttgcgagaa gcggagcgtc
     9001 tgcgggaagt cgaacgtact catgaggctg aacgcgaaat ccttgccctc tgtattgtgc
     9061 gtcctgacct ctcggtagag ctcttccaag agggaatacg agttcctcag ctacaagaga
     9121 atgtgcacct tgaggaatca gattttgtgg atgaagccca cgctaatata ttcgccatcc
     9181 ttaaggagta tgtgggtaag gatattggca gtgttacctc cgaccctcgc gctcgtccgt
     9241 tcctggaccg tcttggagaa ctctctcaag agggggagcg aagaccatcg tcagagggcg
     9301 caatgcaggc ggcttggctg cggctgcgaa tcgtgagccg taagcgtttg atgagggaaa
     9361 cctccgacta tgacgtgaag gatagtttgc gtgctgaggt ggaagcactc aggcaagggg
     9421 tcaccttcgt tgagtcatct tgaaccctta gcctcccaac cgtataatcg cttctgcggt
     9481 attcccctgt agctcaattg gcagagcatt cggctgttaa ccgaagggtt gctggttcga
     9541 gtccagccgg gggagctagg cggtaccacc caggttcccg ccaagggccc atagctcagt
     9601 tggttagagc atccgactca taatcggacg gtcccaggtt cgagtcctgg tgggcccaca
     9661 gatccagaca ctcccacctc ccagaatccc aacttatttc cacatgccca acatgttgtg
     9721 gtccgtctcc tatacgaccg acatgttaca ccagagatac agccttcacc ccaaagagaa
     9781 tcccttcgca acccggcaaa atgcatcttt gaaatagggg tcctataccc tggggtgggg
     9841 ggatgaagct gtgtttaccg gcggaattcg gcctttgagg gccgttttcg ggcgtccaga
     9901 ttgcgcattt tccgtgcata tgccagaatt tcaccgttgt gggtcaaagt ggagcgtagt
     9961 gggaggacac ggctctggcc cttttaggcg agtacgagca cacgctggat gagaaagggc
    10021 gcatgaccct cccttcgcga ctccgct
//
LOCUS       DASTKM010000215         5178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_416621, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000215 DASTKM010000000
VERSION     DASTKM010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416621"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2318)
                     /locus_tag="VFQ10_08955"
     CDS             complement(<1..2318)
                     /locus_tag="VFQ10_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9965508.1"
                     /translation="MAVLPTGTVTFLFTDIEGSTKLWEKSPRDMQVALTRHDALLWEA
                     IEGHGGFVFKTVGDAFCAVFPTALGALESALAAQRTLFSEAWGDEIGALRARIALHTG
                     AAHERDGDYFGPPVNRVARLLSAGHGGQVLLSSSTQELVRDHLPPQTHLRDLGERRLK
                     DLSRPERIFQLTAPDLPSEFPPLKTLETHTNNLPLQATPLIGREREVEAVCELLRSPE
                     TRLLTLLGPGGTGKTRVGLQVAAELVDDFDDGVFFVPIAAITDPTLVAPTIARVLGLS
                     QGGARQAEELLEGYLRDRQALLLLDNLEQVLEAAPVVDRLLSTAAGLKILATSRTPLG
                     LYGEYEFPIPPLSLPDPESLPPLEDLTEYEAVRLFVERAKAVRPDFSLAEDNAPAVVE
                     ICARLDGLPLAIELAAARIKLLSSQVLLDRLGNRLKILTGGARNLPERQRTLRNAIEW
                     SYGLLDEGEKMLFGRLGVFSSGATLEAMEAVCDAEGDLPTDVFEGASSLLDKSLLRQE
                     EGTGGEPRFGMLETIHEFANEKLEESGEADAVSRAHAEYFLALAEEAEPMLWGAEDAA
                     WLDRLEQEHDNMRAALSWAIEHEEATLALRVGAALRWFWYMEGYYGEGRRWLEAALGK
                     NWGAAAAEARARALEGVGWLASSQGDLDRAQAAAKEGLKLSTEAGLGKVIVADFQNVL
                     GDTARHRGDYERAAKLLEKSLALHRAVKDTRGVALSLGDLANVSSDRGSYERARALYE
                     EGLVLARELGGAELLGAYLISLGDEYLLEGNP"
     gene            complement(2589..3440)
                     /locus_tag="VFQ10_08960"
     CDS             complement(2589..3440)
                     /locus_tag="VFQ10_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9965509.1"
                     /translation="MSDDPITGAAPTVNEGDGLQRAGGAGLRRVTFLGTGDALNGERA
                     QSSLALPLSGEKTMLIDASSGTVLLGGLEAAGIPLESVRHLFLSHRHFDHVGGFAPLL
                     ATLASLPEASLLVHAAPDTLRALRNLLDLTLPGAEGWLGKRLGWRELIPGKPTEVGGT
                     EVTPFSLDHGLECVGFRIAQAGAVAVFTSDTRPTQEIVENAKEADLLVHDAYGPHSAA
                     EQAHAMGHSTATEAGEAARAAGVRRLVLTHLRSSRFVDPGALAAEAEAAFGGPVSAAE
                     DLDTVEF"
     gene            3640..4488
                     /locus_tag="VFQ10_08965"
     CDS             3640..4488
                     /locus_tag="VFQ10_08965"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET9965510.1"
                     /translation="MGAEHRGTKNGLAFDLGAFDLDGTVLRRNLRITQGTVAALQGLR
                     ERGMRLVVATGRRFEDAREYAGRLGFAGHDPLICYGGSMVRRINGETLLHRKLPVSLG
                     IEVLEWAAARDLHARVFLDGRIITSPDTPAALDHLRRYAEPGVSVVDLPADWLGECTE
                     QPTKLVIVDRPDDIEGWLEAARTAFDGRLFLTRSLPHYVEIGRLEGTKASALGFLCER
                     WGIEPERVLAFGDADNDIDMLRFAGHGVAVGGMTGEVREAADRVVPGVDEDGVAGYVE
                     TLLGRS"
     gene            4488..>5178
                     /locus_tag="VFQ10_08970"
     CDS             4488..>5178
                     /locus_tag="VFQ10_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9965511.1"
                     /translation="MPELPEVTVISEDVAELAVGREVLRAAVFRPDVTNVEPEEFGRL
                     LVGRTLRATGRRGKIIILDFGEVVGLVHLVISGRVLRLPEWREPDRMNTAVLEFGGGP
                     LVLAFTRLWLGYFDLYEPGEEERHPLISRLGPDPFSKDFTVEYLASVLGRKAGVKGLL
                     LDQSVVAGLGNIYVDEILFSARVHPARKANTLTRDEIQNIHAATREILLRAIELRGTT
                     FDSYHDAFGESG"
BASE COUNT          966 a         1684 c         1591 g          937 t
ORIGIN      
        1 tcagggttgc cttcaagcag atactcgtca cccaggctga tcaagtatgc gccgagcaac
       61 tccgcaccgc ccaactctcg ggccaggacc agaccttctt cgtagagggc cctggcccgc
      121 tcgtagctac cgcggtcgct cgatacgttg gccagatcgc cgagggacaa cgcaacgcct
      181 ctagtgtcct tgacagccct gtggagcgcc aggcttttct cgagcaactt cgccgcccgc
      241 tcgtagtcac cccggtgccg tgcggtgtcc cccagcacgt tctggaagtc ggctacgatt
      301 accttaccca gccctgcctc agtgctcagc ttcagcccct ctttggcagc ggcttgtgcc
      361 cgatcaaggt caccttgact cgacgccagc cagcctaccc cctccagagc cctggcccgc
      421 gcctccgctg ccgccgcgcc ccagttcttg cccagcgctg cctcgagcca tctccttccc
      481 tcaccgtagt atccttccat gtaccagaac caccgtaacg ctgcgcctac tctcagggca
      541 agcgtagcct cttcatgctc tatagcccag gaaagcgctg ccctcatgtt gtcgtgctct
      601 tgttccagcc gatctaacca cgccgcatcc tctgctcccc acaacatggg ctcggcctct
      661 tccgcgaggg ccaggaagta ctcggcgtgc gcacggctca cggcatccgc ctccccgctc
      721 tcctcgagct tttcgttcgc gaactcgtgg atggtctcca gcatcccgaa gcgcggttca
      781 cctcctgtcc cttcctcctg ccgcaagaga ctcttgtcaa ggagcgagga ggcgccttcg
      841 aatacgtccg tggggagatc tccctcagcg tcgcatactg cctccatggc ctccagggtt
      901 gccccgctag agaagactcc aagcctcccg aagagcatct tttcgccctc gtcgagcagc
      961 ccgtaactcc attcgatggc gttcctgagc gttctttgcc tctctggtag gtttcgtgca
     1021 ccaccggtga ggatctttag ccggttgccg agcctatcga gcagcacctg cgaggagagc
     1081 agctttatgc gggccgcggc gagctctatg gccaggggca gcccatctag cctcgcacaa
     1141 atctcaacca ccgccggggc attatcctcc gccagggaga aatccggtct tacagctttt
     1201 gcccgctcca caaagagcct aaccgcctcg tactcggtga ggtcctctag aggcggcagg
     1261 gattcggggt cgggcaacga gaggggtggg atggggaact catactcacc gtagagccca
     1321 agcggggtgc ggcttgtggc caggatcttc aggccggcgg cggtcgaaag cagcctgtcc
     1381 acgactggcg ccgcctccag gacctgctcg aggttgtcga gcaaaaggag tgcctgcctg
     1441 tcgcgcagat atccttcgag caactcctcc gcttgccggg caccgccctg actcagcccc
     1501 agtacccgcg cgatggtcgg ggccaccagt gtaggatccg taatggcggc gataggcacg
     1561 aagaaaaccc cgtcatcaaa gtcgtccaca agctctgctg ctacctgcag tcccacacgg
     1621 gtcttccccg tgcccccagg acccaagagg gtcaacagcc tcgtctcggg actgcgcaac
     1681 agctcacaca ccgcctccac ctcccgctcg cgcccgatga gcggtgtggc ctgcaagggc
     1741 aggttgttgg tgtgggtttc cagggtcttc aagggaggga actccgatgg caggtcggga
     1801 gccgtaagct ggaagatccg ctcgggcctg gagaggtcct tgaggcgtct ctcccccagg
     1861 tccctaagat gtgtctgagg cggcaagtga tcccgcacta gctcctgcgt cgaagaagaa
     1921 agtaagacct gcccgccgtg accggccgag agcagcctcg ccacccggtt caccggtggg
     1981 ccgaagtagt ctccgtctcg ttcatgggcg gctcctgtgt gcagcgcgat acgcgccctg
     2041 agggcaccga tctcatcccc ccacgcctct gagaacaggg tcctttgggc cgcaagcgcc
     2101 gactcgaggg caccaagggc cgtggggaag accgcgcaga aggcgtcgcc taccgtcttg
     2161 aagacgaaac caccgtgccc ttctatggcc tcccagagca gggcgtcgtg acgggtcagg
     2221 gcaacctgca tgtctctggg gctcttctcc cacagcttcg tagaaccctc tatgtctgta
     2281 aacaggaagg tgacggtgcc ggtgggcagg acggccataa gcttctgagc atcctccttt
     2341 gcctgaactc cactatatag tactcccaac aacatgatgc accgctctaa tcgctatgca
     2401 agtcggtgcg ccgctacggt ttgagggtga ggatcacata agacgatctg tgcgagcctt
     2461 gagcagctat tcagcacttc agcaataaag aagctgactg gctgacaagc gaaggccgca
     2521 aggcctgagc gtgcttaaat gctggccagc gcgggttggg gaaggctcga gccttcccca
     2581 acccgctttc agaactccac cgtatcgaga tcttccgccg cgctcactgg ccctccgaac
     2641 gcggcttcgg cctcagccgc caacgctcct ggatcgacga accggctcga gcgcaggtgg
     2701 gtgaggacga gccgccgaac gccggctgcg cgggccgcct cgccggcctc ggtcgcggtc
     2761 gaatgcccca tggcgtgagc ctgctccgca gcgctgtgtg gaccgtacgc gtcgtggacg
     2821 aggagatccg cctccttcgc gttctcgacg atctcctgag tcggacgggt atctgaggtg
     2881 aagaccgcga cggcaccagc ctgggcgatg cggaagccta cgcactcgag accgtggtca
     2941 agcgagaagg gtgtaacctc agtcccgcct acctcggtag gcttccctgg gatcagctca
     3001 cgccagccga ggcgtttgcc aagccagccc tctgcgccag gaagcgtcaa gtcgaggagg
     3061 tttcgcagcg cacggagcgt gtcgggcgcg gcgtgaacga gaagcgaggc ctcagggagg
     3121 gacgccaggg tggcgagcag gggagcgaac ccgccgacgt ggtcgaagtg gcggtgactc
     3181 aagaacaggt ggcgcacgct ctccagcgga atcccggcgg cctcgaggcc accgagcagc
     3241 acggtgccgc tggaggcgtc gatcagcatc gtcttctcac cagagagcgg tagagccagg
     3301 ctagactgcg ccctctcgcc gttaagggcg tcgccggtac cgaggaaggt cacacgtctc
     3361 aagccggccc ctcccgcgcg ttgcagccca tctccttcgt tcaccgtggg agcagccccc
     3421 gtgattggat catcgctcat gtacggcctt gcgtataaca catgaagcag gcggacgcga
     3481 gcatatcatc cagcgcaacg gagaaatcct gaagcgggtc gcgaaccgtc cctaccagca
     3541 atccgcttcc aaagatgcta caaactacct cactgcctta caaaggcaga gttcagggac
     3601 ttcgatccct tcggatctac gacatctgga aggttgcagt tgggcgcgga acacagaggc
     3661 acgaagaacg gattggcctt cgatctcggc gccttcgacc tcgacgggac cgtgttgcgg
     3721 cggaacctga ggatcacaca aggcacagtt gcagcccttc agggtctcag agagcggggg
     3781 atgagactcg tcgtggcgac agggaggcgc ttcgaggacg ccagagagta cgcggggagg
     3841 cttggcttcg cgggtcatga ccccctgatc tgctacggcg gttcgatggt aaggaggatt
     3901 aacggggaga ccctgctgca ccgcaagctg ccagtgagtc tgggcataga ggtgctcgag
     3961 tgggcagcag cacgagatct gcacgcccgt gtcttcctcg acgggcgcat cataacctcg
     4021 ccagacacac ctgcggcgct ggatcacctc cggcgttacg ctgaacctgg cgtctccgtc
     4081 gtggacttac cggcggattg gctcggggaa tgtacagaac aaccgacgaa gctcgtgatc
     4141 gtggaccgcc ccgacgatat agagggctgg ctcgaggcgg caaggacggc cttcgacggg
     4201 cggctcttcc ttacgcgcag cctgccgcac tacgtcgaga tcggcagact ggaaggcacg
     4261 aaggcgtcgg ccctcggctt cctgtgcgag cggtggggga tcgagccgga gcgtgtcctc
     4321 gccttcggcg acgccgataa cgacatagac atgctccgct tcgcggggca cggggtggcg
     4381 gtggggggta tgaccggcga ggtccgagaa gccgccgaca gagtcgtacc cggcgtagac
     4441 gaggacggtg tcgccggcta cgtcgagacg cttctgggaa gatcgtagtg cccgagttgc
     4501 ccgaggtcac cgtcatctcc gaggatgtgg ccgagcttgc ggtaggtcgc gaggtactga
     4561 gggccgccgt cttcaggccc gacgtgacca acgtagagcc agaagagttc ggcaggctgc
     4621 tagtcgggag gacgctgcgt gcgactggtc gcaggggcaa gatcatcatc ctcgactttg
     4681 gggaagtcgt cgggctcgtg catctggtca tctccggaag ggtattgcgt ctgccagaat
     4741 ggagggagcc ggacaggatg aacaccgcgg ttctcgagtt cggaggcgga cccctcgtgc
     4801 tggctttcac gcggctctgg ctcggctact tcgacctcta cgagccggga gaggaagaga
     4861 gacaccccct gatctcacgc ctcggccccg accccttctc caaggacttc acggtcgaat
     4921 acctggcctc agtcttgggc cgcaaggcgg gtgtgaaggg cctgcttctc gaccagtccg
     4981 ttgtggccgg cctcggcaac atctacgtgg acgaaatcct gttctccgcc cgtgtccacc
     5041 ccgcgcgaaa ggcaaacaca cttaccagag atgaaataca gaacatccac gccgcaaccc
     5101 gcgagatcct gcttcgcgcc atcgagctgc gcggtaccac cttcgactcc taccacgacg
     5161 cattcgggga gagtggca
//
LOCUS       DASTKM010000216         4469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_770944, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000216 DASTKM010000000
VERSION     DASTKM010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770944"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1536)
                     /locus_tag="VFQ10_08975"
     CDS             complement(211..1536)
                     /locus_tag="VFQ10_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HET9965512.1"
                     /translation="MEGRRSYDGVGGRETNVMIFAILVFLVVLTVFAVDLVHRTPAAL
                     AGGILLVMAGTIGQEEAIEAVHWETLGLLVGMMILVGILKDSGIFGYLAIKSAQAAGG
                     RPGLVLIYLAAITAFLSAFLDNVTTVLLMFPVTLIISRILEEDPIPFLIVEVLASNIG
                     GTATLVGDPPNIIIGTVVEELTFMDFIVNLTPPILVILLVTLGIVWLAHARKLHTTEE
                     DRKEIMALEAGEEIKDRTLLIRSGAVAGATVVGFFLQQITGFNPAIVALAGAAVAMLV
                     CGPEVEKVLHEVEWPTILFFVGLFVMVGALETTGFIDLVAHYLASASNSLAGTALIVM
                     WGSGFASGIVDNIPFTATMVPVIEQLAENRGYDAATVEPLWWSLSLGACLGGNFTLIG
                     ASANLVVAGLVAREGMTSFTFWRFLLWGFPLTLVALILSSLYVLFFQLG"
     gene            2064..2570
                     /locus_tag="VFQ10_08980"
     CDS             2064..2570
                     /locus_tag="VFQ10_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965513.1"
                     /translation="MSMNIGSFGARLTRDQTSADGVWTKVAFNEVSWDTTEGFDIDTG
                     GYTVGRAGKLHFATGVRLLAPDVPNRYVDLLLFKNGEPRDRLTPDGPFTKPDSVPLAS
                     RETPWAEYVRGFSRGEVVGNPGDLFEIYVRHDFGERADLTLRYPDPADEHTATDRHPI
                     YFMGIWHT"
     gene            complement(2747..3151)
                     /locus_tag="VFQ10_08985"
     CDS             complement(2747..3151)
                     /locus_tag="VFQ10_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9965514.1"
                     /translation="MRPDTLARDALKVMREHNVPGVAVTDEDGTLRGFVTDGQLLDAA
                     LPKYMKMMERLSFVPENADKWVHYLTEAADRPVGEVMSAEVSSIELGRSELAVAHRMV
                     HDGVSSVVITRDGKVEGIVNRLDLYAAIVGLE"
     gene            3292..>4469
                     /locus_tag="VFQ10_08990"
     CDS             3292..>4469
                     /locus_tag="VFQ10_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HET9965515.1"
                     /translation="MQEFFAVALFVVVLALIAFEILNRTVAALLGAAILISFGILDQE
                     QAATEFVDWNTIGLLAGMMVIVAILERTGVFEYLAIKSAQWGEARPGRILILLSLVTA
                     LLSAFLDNVTTVILMVPVTFLIADALESSPIPFLLTQVMASNVGGAATLIGDPPNILI
                     GSAADLSFLDFVFALTPVVLLTLPAVLGVLYLMFRKDLSHSKDAEETIGAMDAAASIR
                     DPILLRKSLIVLGLVIVAFFLHGALHLEAATIALFGAAALMLYARSDVEGILREVEWP
                     TLLFFVGLFVLVGGLEATGFVGSIATALTNVAGGTSALTAIVVIWGSGIASGVVDNIP
                     FTATMIPVIQDLAKAEGLSEEEVRPLWWSLALGADFGGNLTLIGASANVVVAGMSERA
                     G"
BASE COUNT          864 a         1415 c         1308 g          882 t
ORIGIN      
        1 aaatggcggg cagagaaacg ggagccgact aacctacgaa agtgcgcggt gacggccgtc
       61 agttcccctt cccagttgaa gccaagctcg gcccgtaccc tctcccccgg caggtaagcc
      121 atctgcatca cccggtcggg atcgatcatg cgctgtctcc tccttcgatt ctcgtgctgc
      181 aggtgtcgca atgtgtcgca ggttctctac ctacccgagt tgaaagaaca ggacgtagag
      241 gctggagagg atcagcgcga cgagcgtcag ggggaaaccc cagagcaaaa agcgccagaa
      301 ggtaaacgag gtcatcccct cccgcgccac tagcccggcg acgaccaggt tggcggaggc
      361 ccctatgagg gtgaagtttc cccccaggca ggcgcccaga gagagcgacc accacagggg
      421 ctccacggta gccgcgtcgt atcctcggtt ttcggccagc tgctctatga ccggcaccat
      481 cgtcgccgtg aacgggatat tgtccacgat gccggaggcg aagccgctcc cccacatcac
      541 tatcagggcg gtcccggcca atgagttcga ggcggaggcc agataatgtg ccaccaggtc
      601 tatgaaaccc gtggtctcaa gggctccgac catgacgaag aggccgacaa agaatagaat
      661 cgttggccac tcgacctcgt gcaatacttt ctcgacctcg ggaccacaga cgagcatggc
      721 gacggctgcc ccggcgagcg ccacgatcgc ggggttgaag cccgtgatct gctgaagaaa
      781 gaagccgacc accgtcgcac ccgcaacggc gcccgagcgg atgaggagcg ttcggtcctt
      841 gatctcttca cccgcctcaa gggccattat ctctttgcgg tcctcctccg tggtatgtaa
      901 ctttctcgcg tgcgcgagcc agacgatccc caacgtgacg agcaggatca ctaggatcgg
      961 tggcgtgagg ttgacaataa agtccataaa cgtcagctct tcgaccacgg ttcctatgat
     1021 gatgttcggc ggatccccga cgagcgtggc cgtcccccct atgttagacg ccaatacctc
     1081 gacaatgaga aaggggatcg ggtcctcttc cagtatccgc gagatgatca gtgttacagg
     1141 gaacatcagg agcacagtgg tcacgttgtc taagaacgcc gagaggaaag ccgtaatagc
     1201 ggccaagtag atcagtacga ggcccggtcg gccccctgcg gcctgcgcgc tctttatagc
     1261 cagatagccg aagatcccag agtccttgag gatccccacc aggatcatca tccctaccaa
     1321 aagtcccaaa gtctcccagt ggaccgcctc gattgcctcc tcctggccga tggtgcctgc
     1381 catcaccagc agtatcccgc cagccaaggc cgctggcgtg cggtggacga ggtctacggc
     1441 gaatacagtc agcaccacga gaaagaccaa gatggcgaag atcatcacgt tggtctcccg
     1501 accgcccacg ccgtcatagc ttcgccgacc ctccaatacg cgatccgccc ggccgcaagt
     1561 tctctcttta cctcctcacg acgaagccgc tccctgcgac catcctagca gccggccatc
     1621 acacgctcca tacccctgag gtacgtctct ctcctcagac ccacgcgggt agtgcaaaat
     1681 cgggagggta cacttgccct tcaccctacc gtaccttgtg gtgccctcgt cggagacgct
     1741 ggcgtagata acctgccgtt cctggcgacc acggtgccga tgtagagtaa ctcgcgtgat
     1801 ccaagggttc tcggaggcac agctgcagcc tctctcttgg tcgctcgtcc tcgtcgagtt
     1861 cttcgcacag tacttgttct ccctaatggt agctgtggtc tgcgcgctag tccgaactgg
     1921 tacgattagc aatcccagag gcaacctccg agaaacggac cttgtcacgg gaccgtagtg
     1981 ttgaggaagc accgggcggg gtgtccaccg gaggctcaag actgaacgcg cagccgtcgg
     2041 ctgtgcggcc aaggaggcga ggcatgagca tgaacatcgg atctttcggg gcgaggctaa
     2101 cccgggacca gacatcggcg gatggcgtgt ggacgaaggt cgcattcaac gaagtaagtt
     2161 gggacactac agaggggttc gacattgaca ccggcggcta cacggtcggg cgcgccggga
     2221 agttacattt tgctacaggc gtgcggctct tggcgccgga cgtgccaaac cggtacgtcg
     2281 accttttact ctttaagaac ggcgagcctc gagacaggtt aaccccggac ggccctttca
     2341 cgaagcccga ctcagtcccg ctcgcctcca gagaaacgcc atgggccgaa tacgttaggg
     2401 gattttctcg cggcgaggtg gtcggtaacc caggcgacct cttcgagatc tacgtccggc
     2461 acgattttgg tgagcgggcg gacctgacgc tgcgctatcc cgaccccgca gacgagcaca
     2521 ccgcgaccga ccgacatcct atttacttca tgggcatttg gcacacttga tgggtcggcg
     2581 tgacagagga cgcaccggtt ctagtctgga gtcttagtcc ttttcgagag attggttgac
     2641 gccgacttga atagttttgc gaaacgggcg agtatcgtgg ctatgccttc tcgatggaag
     2701 cgttcggcat ctgagcgcca cgaagaaacg gaagagaagt cgcccgttat tccagaccca
     2761 ctatggcggc gtagaggtcg aggcgattca cgatgccctc caccttgccg tccctagtga
     2821 tgaccacgct ggagacacca tcatggacca tcctgtgcgc cacggccagc tcgctcctcc
     2881 ccaactcgat gctcgacact tccgcgctca tgacctctcc cactggccgg tccgcggcct
     2941 cggtgaggta gtggacccat ttgtctgcgt tctcgggaac gaaagagagc cgctccatca
     3001 ttttcatgta tttcgggagc gccgcgtcga ggagctgacc atcggtaacg aatccccgga
     3061 gggtaccgtc ctcatccgtc accgcaacgc cggggacgtt gtgttccctc attaccttga
     3121 gtgcgtcgcg ggcgagcgtg tcgggccgca cggcgtccgc acggcgtccg cggggcgcat
     3181 gatgtcctcg atcttgacgt ctccgcctga ccctccttcg ccgctcatcc cggcctcctt
     3241 agatctccag aatatgtgac ccagcctcca attgtataat ccacagtcag tatgcaggaa
     3301 ttctttgctg tcgcgctgtt cgtcgtggtc ctcgcgctca tcgcgttcga gatcttgaat
     3361 cggacggtcg cagcactact cggcgcggct atcctcatct ctttcggcat tctcgaccag
     3421 gagcaggcgg ccacggagtt cgtcgactgg aacaccatag gcctccttgc agggatgatg
     3481 gtgatcgtcg ccattcttga gaggacaggc gtcttcgagt acctggccat aaagagcgcc
     3541 cagtggggcg aggcacgccc gggacgtata ctcatcttgc tctccctcgt cacggcctta
     3601 ctctccgcct ttctggacaa cgtaaccacc gtgatactga tggtccccgt gacgtttctg
     3661 atcgccgacg ccctcgaatc ctcgcccatt cccttcctgc tgacacaggt gatggcatca
     3721 aacgtgggcg gggcggcgac cctcatcggc gacccgccaa acatcctcat agggagcgcc
     3781 gccgacctat cctttctgga cttcgtcttc gccctcaccc ccgtggtcct gctcactctg
     3841 ccagcggtgc tcggcgtgtt gtacctgatg ttccgcaagg atctgagcca tagcaaggac
     3901 gctgaggaga ctatcggggc gatggacgcg gcggccagca tccgcgatcc tatcctgctc
     3961 cggaagtcgc tcatcgtact cgggctcgtg atagtagcgt tctttttgca cggcgcgctg
     4021 cacctcgagg cggctacaat cgcgctcttc ggagccgcag cgctcatgct ttacgcgcgc
     4081 agcgacgtgg aggggatctt gcgggaggtg gagtggccta ccctactctt cttcgtcggg
     4141 ctcttcgtgc tcgtgggtgg cctagaggcc acagggttcg tcgggagcat agctaccgcg
     4201 ctcacgaacg tcgccggcgg gacgtccgcg ctgacggcga tcgtggtcat ctggggcagc
     4261 ggcatcgcgt cgggcgtcgt agacaacatc ccgttcacgg cgaccatgat cccggtcata
     4321 caggacctcg cgaaggcaga ggggctctcg gaggaggagg tacgccccct gtggtggtct
     4381 ctggccctag gcgcggattt cggcggcaac ctcaccctta tcggggcctc agccaacgtt
     4441 gtcgtggccg gcatgagcga gcgcgccgg
//
LOCUS       DASTKM010000217         5851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1300236, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000217 DASTKM010000000
VERSION     DASTKM010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300236"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VFQ10_08995"
     CDS             complement(<1..999)
                     /locus_tag="VFQ10_08995"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HET9965516.1"
                     /translation="MRLIISEKANTAKKIAQFLSEGPVKEGKHRSVPHHTFTWKGEET
                     VSVGLKGHVLNPEYPEEYSNWQKVEPRELIDAQILKSVSEKGVAQAVTSLAKKADRVV
                     IATDFDREGELIGVEALSLVFEANPKLMDHVERARFSALTPGEVGRAFDELMEVSNDL
                     ADAGEARQDIDLIWGATLTRWVSRATKRYGSAFLSVGRVQSPTLVLIADRERERRAFV
                     PVPYWELEVNLKNGEPFTVRHQTERFEDEERAKRAYENVADTAIVVEVEQKSATRRPP
                     TPFNTTAFLTAAANIGISPSRAARIAEDLYTDGFISYPRTDNTVYPRSLDLREVLGY"
     gene            complement(1378..2652)
                     /locus_tag="VFQ10_09000"
     CDS             complement(1378..2652)
                     /locus_tag="VFQ10_09000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9965517.1"
                     /translation="MANRWDRIALASVLAGVIVRIIWGLVIHPPLDYLYSDMGAYVER
                     AQRLATGAGLQRSDAFFPPGTHVLLAAPMTLFGTERAGLWGAAVLWCALSAAIPFFAW
                     RLARLLLTPAAAALTALFCAFWPLYITYGGYFTSETPSLAFLLASLWAGYRAVRLSGR
                     EAGWLGLAAGLLGGVAVANRPQLILNLAVLVVPLLFRLRRQALALTGIVVGSAFILAV
                     TALHNSAAAGKPTGLSENAGLNFWIGQCDVHDVTTVDPVRNITFQFGNPVWTQLGRGG
                     SYYFEGHLVWDQGFFYEMGLRCIERDGLGHVRVLARNVLNMSATTIPWPQVNEAGQRG
                     VVRLSNLAYSILLPLIVIDSLFVLVRRPASRRSSGEAVMLLQLACALLVAIFFFGDPR
                     VRSSYDVFGLALLAARVADRFGLEESSGKPAE"
     gene            complement(2857..4542)
                     /locus_tag="VFQ10_09005"
     CDS             complement(2857..4542)
                     /locus_tag="VFQ10_09005"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET9965518.1"
                     /translation="MSGTPKDWSIERWAYAKVVEIFGADLRSLATFRIVLALLVLADL
                     ANRATDISAHYTDAGIMPRSVLVEQLLSPWAFSLHLMNGGRSFQALLFGVAALAAFGM
                     LVGYRTRLMTFVVWVLLLSIQVRNPLVNGSESTMLRMLFFWGMLLPLGAYWSVDRARS
                     ALSRPSPRFLSLATFGLFLQIAFVYWFTAALKSGPEWRTDYTALYYALSLDQLATPIG
                     HLLLDFPSLLQVLTFGTFLLEAIGPLLLFCPFFTGPVRTGAALAFMSFHFGIWLTMDI
                     GIFPWISAFCMVCFFPTWFWDRASTLRSVLLRRVELARRLQLATARLANAIVSFSKAV
                     LSFLMDAGQLLFAGPALHGDPLADHTAGPVPPPAAGTIGTAETRHVEEPEPAELRSSL
                     ATNLLAFFFIFYILCWNLTTATSFTLPERTVPLGPFLGLDQYWGMFSPSPSDEDGWYV
                     IPGELRDGQKVDLMPVTRDDYSMHPISYEKPQDIPATYKNEHWRKYLENIYNQDHADQ
                     RLYFGQYICRQWNAHHTGAETLETFRIIYMLEVTLPDYRQSKPEKVDLWNHEC"
     gene            complement(4639..5532)
                     /locus_tag="VFQ10_09010"
     CDS             complement(4639..5532)
                     /locus_tag="VFQ10_09010"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HET9965519.1"
                     /translation="MFREGNSLLIKVGLIMVALAFALVIGAIVVSVTLGSEAGRVATD
                     IAKKSVREAQRDASGKQEESLGYGSSSSAESMRQRKEDAKEPVAAASEPERSYSRSEM
                     PQPAGQETPSELELRPQRHQTQAQEQPLPAAEDAGWPAPTQEELQGANGERHYELLPG
                     AVMGLTIHSIGVYNAPVFDSDSQWALATGVAHVPETSLPWSQSAQRNVYLAGHRMGYR
                     GTWSRMIFYNLDKLKEGDKVVLKDRTGRPYEYRVSETFLAEPSDSWVMGQVRGRDMVT
                     LQTCTPIPTFQKRLIVRADRV"
     gene            complement(5560..5697)
                     /locus_tag="VFQ10_09015"
     CDS             complement(5560..5697)
                     /locus_tag="VFQ10_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965520.1"
                     /translation="MIRETIPNDGVLPDTGGLSTLVPVGAVLALIISGSAIGLFYVRR
                     R"
BASE COUNT         1123 a         1777 c         1892 g         1059 t
ORIGIN      
        1 gtacccgagc acctcgcgca ggtcgagcga gcgcgggtag acagtgttgt ccgtcctggg
       61 atatgagatg aagccatccg tgtacaggtc ctcggctata cgggccgccc tggaggggct
      121 gatcccgatg ttcgcggccg cggtcaggaa ggctgtggtg ttgaacggcg ttggtggcct
      181 gcgggtcgcg gacttctgct cgacctcaac gactatcgcg gtgtcggcga cgttctcgta
      241 ggctctcttc gccctctcct cgtcctcgaa gcgctcggtc tggtggcgga cggtaaacgg
      301 ctcgccgttt ttcaggttta cctccagctc ccagtaaggc acagggacga aggcacgccg
      361 ctcgcgctca cggtcggcta tgaggaccag cgtcggcgac tggacgcgtc ctaccgagag
      421 gaaggcgctc ccgtagcgct tcgtggcgcg tgagacccag cgggtcaggg tcgcgcccca
      481 gatcagatct atatcctgcc gcgcctcgcc ggcgtcggcc aggtcgttgg agacctccat
      541 cagctcgtcg aaggcgcggc cgacctcgcc cggcgtgagg gcggagaagc gggcacgctc
      601 gacatggtcc atgagcttcg gattagcctc gaagaccagg gagagggctt cgacgccgat
      661 caactcccct tcacggtcga agtccgtggc gatgaccacc ctgtcagcct tcttcgcgag
      721 cgacgttacg gcctgggcca cgcccttctc cgagacggac ttcaggatct gggcgtctat
      781 gagctcccgc ggctcgactt tctgccagtt cgagtattcc tcgggatact ccgggttgag
      841 gacatgcccc ttcagaccaa ccgaaaccgt ctcctccccc ttccacgtaa acgtgtggtg
      901 cggcacagag cggtgcttgc cttccttgac aggcccttca gagaggaact gggcgatctt
      961 cttcgcagta tttgctttct cggagataat tagtcgcatc ttgttaccat tttacaccga
     1021 caaagagcat tttgtcggca gatagcaagt ccttgttctt cgaggcggcc ctgttctcga
     1081 aggaccatga ctggtctctg ctgtgacctt gcagcgtacc ctcccatcct agctactgtg
     1141 cgcagacctc ccacaaatct atcctcggcg gaggacgtcc ctttggtgta taaaccactg
     1201 ctgccagtat ttcaatcggc tcgagattag cgtgcccgag atcaggaacc cgaagggtcg
     1261 acgctcaggg cgcattgatg tgcaacgttt acgggattac tttaggacgc cgcaggggta
     1321 agcctcgagg gcgacctaaa gccaagtcgg gaactggttg ctgacgggca aggggcccta
     1381 ctcagcgggt ttcccacttg actcttcgag gccgaaccgg tcggcgacgc gcgcggcgag
     1441 cagggcaagg ccgaagacat cgtaggaact gcgcacgcga gggtcgccga agaagaagat
     1501 ggccaccagc aacgcgcagg ccaactggag cagcatcacc gcctcccccg acgaacggcg
     1561 ggatgcgggg cgtcggacga ggacgaagag ggagtcgatg acgatcagcg gcagcagaat
     1621 gctgtacgcc aggttgctaa gccgcaccac gccccgctgt ccagcctcgt tgacctgcgg
     1681 ccagggaatt gtcgtggcgc tcatgttcag gacgttcctg gccaggaccc gaacgtgacc
     1741 gaggccgtcc ctttcgatac accgcagacc catctcgtag aagaatccct gatcccatac
     1801 caggtgtccc tcgaagtagt agctgccgcc ccgccccagt tgcgtccaga caggattgcc
     1861 gaattggaag gtgatattcc ggacaggatc gacggtggtc acgtcgtgga cgtcgcactg
     1921 tccgatccag aagttcaggc ccgcgttctc gctgaggcca gtgggtttgc cggccgccgc
     1981 gctgttgtgc aacgcggtga cggccaggat gaacgcgctg ccgacgacga tgccggtgag
     2041 agccagggct tggcggcgga gacgaaacaa caacggcacc accagcaccg cgaggttcaa
     2101 gatgagttgc gggcggttgg cgaccgctac gcccccgagc aggccggctg ccaggccgag
     2161 ccagcctgcc tccctgcccg agagccggac ggcccggtag ccggcccaca gagaagcgag
     2221 caaaaacgcc agcgagggcg tctccgaggt gaagtaccct ccataggtga tgtacagggg
     2281 ccagaacgcg cagaaaagcg cggtcagcgc tgcggcggcc ggggtgagta acaagcgcgc
     2341 caggcgccac gcgaagaacg ggatcgcagc ggagagcgca caccacagca cggccgctcc
     2401 ccacaatccc gcccgttccg tcccgaatag cgtcattggg gcggccagca gtacgtgggt
     2461 ccccggcgga aagaacgcgt cggagcgctg caggccggcg cccgttgcga gccgctgggc
     2521 cctctcgacg tacgcgccca tgtccgagta gagataatcg aggggagggt ggatcaccag
     2581 cccccagatg atacggacga taacgccggc cagaacggac gccagggcga tcctgtccca
     2641 ccggttcgcc atcacttccc gaacggcgcc cggcgccttc ccttccgcgc cgaccggctg
     2701 cgtcccctcg ctagagactc gccgttattc ggggcatcgg tgtgacgatc gaaaccggtt
     2761 ttggccagag gttcggcgtc catcagctac ggatcgggcg cgtgcggcgg acacggccct
     2821 ggcaggagct ccgtctgcgg tcgggcgcac gtgtggctag cattcgtggt tccagaggtc
     2881 caccttctcc ggtttggact ggcggtaatc cggaagtgtc acctccagca tgtagatgat
     2941 ccggaaggtt tcgagggtct ccgcgcccgt atgatgggcg ttccactggc ggcagatgta
     3001 ctggccgaag tacaggcgct ggtcggcgtg atcctggttg tagatgttct ccaggtactt
     3061 acgccagtgc tcgttcttgt aggtggcggg aatgtcttgt ggtttctcgt aagagatggg
     3121 gtgcatgctg tagtcgtcac gggtgacagg catgaggtcc accttctggc cgtcgcgcag
     3181 ttcgccgggg atcacgtacc agccgtcttc atcgctcggg gagggggaga acatccccca
     3241 gtactggtcg aggccgagga aggggccaag gggaacggtg cgctcgggca acgtgaacga
     3301 ggtggctgtg gtgaggttcc agcagaggat gtagaagata aagaagaagg cgagcaggtt
     3361 ggtggcgagc gaagaccgca gctccgctgg ttctggttct tcgacgtgcc gcgtttcggc
     3421 cgtgcctatc gtcccggccg ccgggggcgg tactgggccc gcggtgtggt cagccagcgg
     3481 gtcgccgtgc agcgcgggac ctgcgaacag gagttgcccg gcgtccatca ggaacgacag
     3541 gaccgccttg gagaaggaca cgatagcgtt ggccaaccgc gccgtggcga gttgcaggcg
     3601 gcgggcgagc tcgacccgcc gcaacagcac ggagcggagc gtcgaagccc tgtcccagaa
     3661 ccaggtgggg aaaaagcaga ccatgcagaa cgcgctgatc caggggaaga tccctatatc
     3721 catcgtcagc caaatgccga agtggaagct cataaaagcc agcgcggccc cggtgcggac
     3781 cggaccggtg aagaacgggc agaacagcag gagcggcccg atggcctcga gcaagaacgt
     3841 gccgaaggtc agcacctgga gcaagctggg gaagtcaagc agcagatggc cgataggcgt
     3901 ggcgagctgg tccaggctga gcgcgtagta gagcgcggtg taatccgtgc gccactcggg
     3961 tcccgacttc aaggcggcgg tgaaccagta gacgaaggcg atctgcaaga acagtccgaa
     4021 ggtggccagc gacagaaagc gtggcgaagg acgcgagagc gcagaccgcg cgcgatccac
     4081 cgaccagtag gcgccgaggg gcaacaacat tccccagaag aacaacatgc gcaacatggt
     4141 gctctcggag ccgttgacca gcgggttgcg cacctgaatc gagagcagca acacccacac
     4201 gacgaaggtc atcaggcgcg tgcggtagcc gacgagcatg ccgaaggccg cgagcgccgc
     4261 gacgccgaac aacagcgcct ggaacgacct ccccccgttc atgaggtgga gtgagaacgc
     4321 ccaggggctc aacagctgct ccacaagaac gctgcggggc atgatgcccg cgtcggtgta
     4381 gtgtgcggag atgtcggtgg cccgattggc gaggtcggcg agcacaagga gcgccagcac
     4441 gatacgaaac gtcgcgagtg agcgcaggtc agcgccgaag atctccacaa ccttcgcgta
     4501 cgcccaacgc tctatggacc agtcctttgg tgtgccgctc acgttgaaag gtccttgtct
     4561 cttacatagc cgagatcgaa aagggggagg gagaccgtgg attgcccctg gcagcgtcgt
     4621 actgtgggag gctgtgatct atacgcggtc tgcacgtacg atcagacgct tctggaaggt
     4681 agggatcggg gtgcatgtct gcaacgtcac catgtcccta cccctcacct gccccatcac
     4741 ccacgaatca gagggctcag ccaggaacgt ttcgctcacc cggtactcat aaggacgccc
     4801 tgtacggtcc ttgagcacta ccttatcgcc ctccttcagc ttgtccaggt tatagaagat
     4861 catacgactc caggttcccc tataacccat gcgatgtcct gccaggtaga cgtttctctg
     4921 agcactctgt gaccatggca gggaggtctc tgggacgtgg gctacgcccg tggccagtgc
     4981 ccactggcta tctgagtcga acacgggagc attgtatacg cctatggagt ggatagtgag
     5041 gcccattacc gccccgggaa gcagttcgta gtgacgttct ccgttggcgc cctgaagctc
     5101 ctcttgggtt ggcgcgggcc agccggcatc ttccgccgcc ggcagaggtt gctcttgcgc
     5161 ctgtgtctgg tgccgttgcg gtcggagttc cagctcagag ggcgtttcct ggcccgccgg
     5221 ttgaggcatt tcggatcgag agtacgagcg ttcgggttcg gatgcagcgg ccaccggctc
     5281 cttagcatcc tccttgcgct gcctcatcga ctcagcagag gaggatgagc cgtatcccag
     5341 cgactcctcc tgcttcccag aagcgtctcg ctgcgcttcg cgaacagact tcttcgcaat
     5401 atctgtcgcc acccgcccag cctcgctgcc tagagtgacg ctaaccacta tcgcgccgat
     5461 cacgagggcg aaggcgagcg ccaccatgat caggcccacc ttaataagaa gcgagtttcc
     5521 ctctctgaac actcgcctac cttcctacaa tggagagtgc taccgccggc gcacgtagaa
     5581 caaccctatg gccgagccgc tgattatcag cgccagcact gcccccacag gaacgagcgt
     5641 ggagagccca cctgtgtctg gtagtacccc gtcattgggg atcgtctcgc gaatcacgcc
     5701 gtcgtttgcg gaggtgctat tgcctgagtt ggtcgttgct ccagtagtag tagtagtgtt
     5761 cgctgcaccg gtgctagctg cggtggtcgt tgctgttgtc gcatctgtag tgtccgtggt
     5821 actcgtagtg gtggtagtgg tccccgttgt c
//
LOCUS       DASTKM010000218         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1124185, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000218 DASTKM010000000
VERSION     DASTKM010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124185"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..884)
                     /gene="rplB"
                     /locus_tag="VFQ10_09020"
     CDS             complement(45..884)
                     /gene="rplB"
                     /locus_tag="VFQ10_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET9965521.1"
                     /translation="MPARRYRPTSAGRRNSSVLTRDSVTKNDPEKKLTAKLHKTGGRN
                     NHGRITTRHIGGGHKRRYRLIDFKRRKDGVPATVAAIEYDPNRSANIALLHYHDGEKR
                     YILAPRNLTVGSIVESGPDAEVDVGNALPLNRIPVGTVVHAVELEPGRGAQMARSAGT
                     SAQITAREGDLVTLRLPSGERRRVRAECRATVGVVGNQSHQNVRWGKAGRKRHLGIRP
                     TVRGTVMNPVDHPHGGGEGKSTPGRAPVTPWGKITQGSPTRKKHKRSDAMIVRRRRKG
                     RRR"
     gene            complement(886..1173)
                     /gene="rplW"
                     /locus_tag="VFQ10_09025"
     CDS             complement(886..1173)
                     /gene="rplW"
                     /locus_tag="VFQ10_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET9965522.1"
                     /translation="MDPHKVIIRPVISEKSYNLIETEGQYTFHVDRRANKNQIKRAVE
                     GAFDVSVQKVNTVNVKSKPKRQGLTRGRTPMWKKAVVKLAEGERIELFEGV"
     gene            complement(1174..1803)
                     /gene="rplD"
                     /locus_tag="VFQ10_09030"
     CDS             complement(1174..1803)
                     /gene="rplD"
                     /locus_tag="VFQ10_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET9965523.1"
                     /translation="MAKAQVFDARSGDTSSLDLQGPRFTTEPNEHVIHRAVVAELDSR
                     RRYTASTKGRSEIRGSGAKLYRQKGTGRARAGDAKSPIRQGGGTWGGPKAKPPRYGKR
                     INRKEATAAFDGALSAKAQDGEIFVLDSLAFDTPSTKQAKELLSRMGLEGPVLVVLTE
                     SDANAALSFRNLPRVSVVGPHEYGVYELLRAREVVFSRAAYSRLAGKEG"
     gene            complement(1805..2422)
                     /gene="rplC"
                     /locus_tag="VFQ10_09035"
     CDS             complement(1805..2422)
                     /gene="rplC"
                     /locus_tag="VFQ10_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET9965524.1"
                     /translation="MATAVFGRKKHMTQVFQDDGTLVGVTVIEVEPNLITAVRTAEED
                     GYTAVQVGFGAAKSSRVDKARAGTFAKLDTPPRRYLKELRDVSGEVGESIGVDLFEVG
                     DRVHVSATSKGKGFQGTIKRHGFARGPMSHGSHNIRQPGSVGASADPARIFRGQKMPG
                     QMGNQTATVRNIEVVAVDADNNQLWVRGGVPGGKNAVVRIRKVED"
     gene            complement(2427..2738)
                     /gene="rpsJ"
                     /locus_tag="VFQ10_09040"
     CDS             complement(2427..2738)
                     /gene="rpsJ"
                     /locus_tag="VFQ10_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET9965525.1"
                     /translation="MAVSKIRIKLKAYDHEVIDKTARSIVETAERTGAFVFGPIPLPT
                     KRSRYTVIRGPYRHKDSREHFKLHTHKRLIDIQQPTPRTVDSLQRLDLPAGVNIEIKA
                     T"
     gene            complement(2742..>2881)
                     /gene="tuf"
                     /locus_tag="VFQ10_09045"
     CDS             complement(2742..>2881)
                     /gene="tuf"
                     /locus_tag="VFQ10_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET9965526.1"
                     /translation="MVMPGDNTVMNVELISPIAMDEGLNFAIREGGRTVGAGVVTQIV
                     E"
BASE COUNT          397 a          969 c          881 g          634 t
ORIGIN      
        1 aggcgctcct ccacgaacgg ctctttctta gaggaccgcg tcatctagcg cctccttcct
       61 ttcctgcgcc ggcggacgat catggcgtcc gagcgcttgt gcttcttcct cgtcggggag
      121 ccctgggtga tcttgcccca aggcgtaacc ggggcccggc ccggcgtgct cttcccctcg
      181 ccgccaccgt gcgggtggtc gacggggttc atgaccgtac cgcgcaccgt aggccttatc
      241 ccgaggtggc gcttgcggcc agctttgccc cagcgcacat tctggtgcga ctggttgccg
      301 accacgccaa ccgtggccct gcactcggcc cttaccctgc ggcgctcgcc gcttgggagc
      361 cttagcgtca ccaggtcacc ctcgcgggcc gttatctggg cgctcgtccc ggcgctgcgg
      421 gccatctgcg ccccgcggcc aggctcgagc tccacggcgt gcacgacggt accgacgggt
      481 atccggttga gggggagcgc gttgcccacg tccacctcgg catctggccc gctctcgacg
      541 atggagccga ccgtgaggtt gcgcggcgcg aggatgtagc gcttctcgcc gtcgtggtag
      601 tgcaagaggg cgatgttggc cgaacggttc gggtcgtact cgatcgccgc aaccgtcgcg
      661 ggtacaccgt ccttgcgccg cttgaagtcg atgagacgat agcggcgctt gtgtccgccg
      721 ccgatgtggc gggtggttat gcggccgtgg ttgttgcgcc cgcccgtctt gtgcaacttg
      781 gcggtcagct tcttctcggg atcgttcttc gtgacagagt cgcgcgtcaa gaccgaagag
      841 ttcctgcggc ccgcgctagt cggcctgtaa cgtctcgcag gcatactaga ccccctcgaa
      901 taactctatg cgctcgccct cggccagctt gactacggcc ttcttccaca tcggcgtcct
      961 gcccctggtg aggccctggc gcttgggctt gctctttacg ttgaccgtgt tcaccttctg
     1021 gacgctaacg tcgaacgcgc cctccacggc ccgctttatc tggttcttgt tcgcccgccg
     1081 gtctacgtgg aaggtgtact gcccctcggt ctcgatgagg ttatagctct tctcggagat
     1141 gaccggcctt attatgacct tatgggggtc catctagccc tcctttccgg cgagcctgga
     1201 gtaggcggcc cgcgagaaga ctacctcccg cgccctgagc agctcgtaga ccccgtactc
     1261 gtggggtcct accacgctga cccgcggcag gttcctgaag gagagcgccg cgttcgcgtc
     1321 gctttcggtc aggacgacga gcacgggacc ctccagaccc atcctggaca gaagttcctt
     1381 ggcctgcttc gtggaaggcg tgtcgaaagc cagggagtcg agcacgaaga tctcaccgtc
     1441 ctgcgcctta gccgagagcg ccccgtcgaa cgctgctgtg gcctctttgc ggttgatccg
     1501 cttcccgtaa cgcgggggct tggccttcgg gccgccccag gtgccgccgc cctggcgaat
     1561 aggtgacttg gcatcgccgg cgcgggcgcg tcccgtcccc ttctgacggt acagcttggc
     1621 tcctgagccc cggatctcac tccgtccttt ggtcgaggcg gtgtaacgcc gcctggagtc
     1681 gagctcggcg acgacggccc tgtgtatgac gtgctcgttc ggctcggtcg tgaagcgcgg
     1741 gccctggagg tcgagcgagg acgtgtcgcc gctgcgggcg tcgaaaacct gtgccttggc
     1801 catcctaatc ttccaccttc cggatcctga cgacggcgtt cttgccgcct ggcacaccgc
     1861 ctcgcaccca gagctggttg ttgtcggcgt cgacggcaac aacctcgatg ttgcgcaccg
     1921 tggccgtctg gttacccatc tggcccggca tcttctgtcc cctgaagatc ctggccgggt
     1981 cagctgacgc cccgacggag cctggctgcc tgatgttgtg cgagccgtgg ctcatagggc
     2041 ccctggcaaa accgtggcgc ttgatggtcc cctggaaacc ctttcctttg gacgtggcgc
     2101 tgacgtggac cctgtcgccc acctcgaaga ggtccactcc tatgctctca cccacctcgc
     2161 cactcacgtc ccgcagctcc ttcaggtacc tgcgcggggg cgtatcgagt ttggcgaacg
     2221 tgccagccct ggccttgtcg acccggctgg acttggctgc cccgaagccg acctgtacgg
     2281 ccgtatagcc atcctcctcg gcggtacgta cagccgtgat caggttcggc tcgacctcga
     2341 ttacggtaac gccgacgagc gtgccgtcat cctggaagac ctgggtcata tgctttttgc
     2401 gtccgaatac tgcggttgcc ataaccctac gtcgccttta tctctatgtt gacgcctgcg
     2461 ggcaggtcga gccgctgcag cgagtcgacc gttcgcggcg tgggctgctg gatgtctatg
     2521 agacgcttgt gcgtgtgcag cttgaagtgc tcgcgggagt ctttgtgcct gtagggaccc
     2581 cggatcaccg tgtacctgct gcgcttggtg ggcagcggga tggggccgaa cacgaaggcc
     2641 cctgtgcgct ccgccgtctc gactatcgag cgggcagtct tgtctatgac ctcgtggtcg
     2701 tatgctttta gctttatcct gatcttcgat acggccacag gttactccac gatctgtgtt
     2761 acgacgcctg ctcctactgt ccttcctccc tctcggatgg caaagttcaa cccctcatcc
     2821 atcgcgatcg gtgagatcaa ctccacgttc attaccgtgt tgtcccctgg catcaccatc
     2881 t
//
LOCUS       DASTKM010000219         8854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_243658, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000219 DASTKM010000000
VERSION     DASTKM010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8854
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243658"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..834
                     /locus_tag="VFQ10_09050"
     CDS             226..834
                     /locus_tag="VFQ10_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9965527.1"
                     /translation="MQKPRWKRAEALRGEGRPYAIPVGGSNAVGALGYVECARELYTQ
                     AHKLDLRIDALVHASASHGTQAGLAVGLAASGPEARLLGVGVSGSAQQARANVERIAG
                     TTSERLGSPQRFAEIEVDDRFLGEGYGIPTPEGLAAIRLLARLEGLLLDPVYTGKAMA
                     GLIAMSREGRFSDEQNVVFLHTGGWPALFAYEEELAEEEAPR"
     gene            complement(1067..3835)
                     /locus_tag="VFQ10_09055"
     CDS             complement(1067..3835)
                     /locus_tag="VFQ10_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9965528.1"
                     /translation="MTGLPTGTITFLFTDVEGSTRLWERGPEAMSQALSRHDELLREA
                     VEFRGGFVFKTVGDAFCVAFPNAPEAVEAAHDAQKFLLSEEWEETGPLKVRMALHTGT
                     AEERDGDYFGPTINRVARLLSAGHGGQILLSLTTQELVRDQLPVGAGLRDLGVRRLKD
                     LFGPERVFQLTAPDLPASFSPLKTLDVRLNNLPIQPTPLLGREREVSEITDLLRRADV
                     RLLTLTGTGGTGKTRLALQSAAELIDEFEDGVFFVALAPISDPKLVSSTMAGALSVSE
                     SAGRDLKEELRDYLSNKELFLVLDNFEQVADAAPLVGELLSGCPNLKVLVTSRTLLRL
                     YGEHEYAVRPLELPDPGYLSPFETLRGYEAIRLFAERARAADADFSLTNENAPAVAEI
                     CARLDGLPLAIELAAARIKLLSPQAMSSRLGNPLKFLTGGARNLPERQRTLRGTIAWS
                     YALLNEGEQALFASLSVFSGGCALDAVEAVCDAEGDVFVEVLERLSALLDKSLLRQEE
                     GVEEEEPRFVMLETIREYARERLEVSGNAEVIRRLHAEYFLALAEQGASELQGPEEAT
                     WLERLEMDHDNMRAALSWTLQSEEAELGMRLAGALWQFWDMRGYYGEGRRWLEEALAK
                     DGRASVARVKALEGVGWLADLQGDIDRAVAAAEEGLRLGARAKLQSSDKASFLRILGS
                     AAYVCGDHERATRLYEESLALSREAGDERGVASSLLQLGNVSSDLGDHERAETFYEEG
                     LALSRELDDKALLASALISVGAEYLLQGDHERGAMLNEEAAGLYRERGNRGHLQYALD
                     NLGWAALMRGNLQQAEALHRESLALSGQLGDKLVAAEALEGLACSASARGEAAERVAR
                     LFGAAESLRGTVGYRQETREHALREPYLAAARAQLSEARWDAAWAEGRRLGFEEAIAY
                     ALEKAWGG"
     gene            complement(4063..4680)
                     /locus_tag="VFQ10_09060"
     CDS             complement(4063..4680)
                     /locus_tag="VFQ10_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9965529.1"
                     /translation="MKEPAREGLLVDGTGAPLAEEDSGSVELPDGSSIAYHAIAPDNA
                     SALQRFHHRLSKRSIYLRFFAAKPELSDREAGYFTNVDGINRFALVAVDPERPEEIIG
                     VVSFDREGTTERAEYAAEVEDRWQGRGLGLALTRHLIEAALKRGVRTFTGIVLPENTR
                     MLHLLRDLNLPERLRYEDGTDYVEIDLSPLQNTPDSAAGASGPHG"
     gene            4989..5324
                     /locus_tag="VFQ10_09065"
     CDS             4989..5324
                     /locus_tag="VFQ10_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965530.1"
                     /translation="MDHERTEKELEEIFEEIQHYYKTFADDALALQERTLQLARELLE
                     ICAGREASGMRDTLENLAERSRGERERFERLERKAGEAYTRVLKGPVDEHHHKIEEAE
                     ADLKEAGSS"
     gene            complement(5371..6009)
                     /locus_tag="VFQ10_09070"
     CDS             complement(5371..6009)
                     /locus_tag="VFQ10_09070"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HET9965531.1"
                     /translation="MLGTEDDTAQLLDELVSSAGADPTSASYEHASLRETKRYLAKLG
                     DAMAEGDDRLGETLQGEPSPQGQTFSKSEFFGRPLPAETIAALVENLTEGRISGQSRE
                     LDFTPWGGAYNRVSADATAFVHRDERFLLKHAVVIDPDASRGERKTARRWLSRSWASV
                     RPWGSGRVYPNFPDPDLEDWAQAYYGTNYDRLLRVKAQYDPGNIFFFQQSLK"
     gene            complement(5990..6850)
                     /locus_tag="VFQ10_09075"
     CDS             complement(5990..6850)
                     /locus_tag="VFQ10_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9965532.1"
                     /translation="MRSGGRRDMVPSAPDWEALQRAVSGDVVLPGSPDYESTRKPAIA
                     NFHDIRPQAVVLCRTPQDVSETISFARESGSRTAARSGGHCFAGRSSTEGIVIDVTPM
                     RSVSVSDGVATVGAGARLGEVYDTLAQYGLTIPGGCGPSVGISGLALGGGLGILGRKH
                     GLTSDHLLGLQIVLADGRVVVCDEQHDEELFWALRGAGGGNFGVVTSLIFGTLPAPAA
                     TGFHLLWPHTHAAALISAWQAWAPAAPDELAASLLVTASRDPERPPVVNVSREAARCQ
                     RVRLHARHRR"
     gene            complement(6843..7103)
                     /locus_tag="VFQ10_09080"
     CDS             complement(6843..7103)
                     /locus_tag="VFQ10_09080"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9965533.1"
                     /translation="MIAQSYLAADVRSTSWAEARRRLAEADAYWLATVRSNGRPHLVP
                     VLAVWLDDALHFVASRTSRKAKNLARDSQGGAFSVSKVGPGA"
     gene            complement(7208..8653)
                     /locus_tag="VFQ10_09085"
     CDS             complement(7208..8653)
                     /locus_tag="VFQ10_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9965534.1"
                     /translation="MTDNRQDTKRLSPIKPLDEAVIRAFSSKVRGQLIRPQDDGYDEA
                     RTVFYGGFDRHPALIVRVADPTDVSHVITLARERGLELAIRSGGHSVAGHSTTEGGIV
                     LDLSEMKNLEIDTEGRTAWAETGLTAGEYTAAVGAHGLATGFGDTGSVGIGGVTLGGG
                     VGYLVRKHGLTIDDVLAAEIVTADGELLRVDAESHPDLFWAIRGGGGNFGVVTRYHYR
                     LHEVSTIVGGMLVLPATPEVIASFIAEAEAAPEELSTIANVMVAPPMPFLPAEAHGQL
                     IIMAMLVYAGEVEAGERAIAPFRALAEPIADMVRPMPYPEIYPPEEGDYHPTAAARTM
                     FVDKIDRDVAEMIVEYLLASDAVMRVAQLRVLGGAMARVPAEATAFAHRSSRIMVNVA
                     AFYEGPEDRVAREAWVSDFAAALRQREDGAYVGFLGEEGEGRVREAYPGSTWERLAGI
                     KGRYDPTNVFRLNQNIRPSTQPESDAASTIT"
BASE COUNT         1642 a         2899 c         2654 g         1659 t
ORIGIN      
        1 taggcgacgc cctcgtccga ggcgccgaca cggtgatgac cgtcggggcc gtgcaatcca
       61 atcacgccag gcaaaccgcc gcggcttgcg cgaggttggg cctgagatgt gagctgtttc
      121 tcaaggagtc agcgccgggc cgtggcgaaa gctatgggca ctcgggcaat gtgctgctcg
      181 accgtatcct cggcgcgaaa ctccacatca tcccgggcgc ctccaatgca gaaaccgcga
      241 tggaagcgcg ccgaggcttt gcgcggggaa gggcggccct acgcgatccc ggtcggcggg
      301 tcgaacgccg tgggcgccct cggctacgtc gagtgcgcgc gcgaactcta cactcaggct
      361 cataagctgg acctccggat cgatgccctg gttcacgcga gtgctagtca cgggacccag
      421 gcagggctag ccgtgggcct ggcagcaagc gggcccgaag cgcggctcct cggcgtgggc
      481 gtcagcggct ctgcccagca ggcccgggcg aacgtcgagc gcatcgccgg taccaccagt
      541 gaacgtctcg gcagcccaca gcgcttcgct gagatcgagg tcgacgaccg tttcctcggc
      601 gaaggctacg gcataccaac accggaggga ctggcggcta tccggctcct ggctcggctg
      661 gaggggctcc tgctcgatcc ggtctacacg ggcaaggcga tggccgggct aatcgcgatg
      721 agtcgtgagg gccgattctc ggatgagcag aacgtcgtct ttttgcacac cggcggatgg
      781 ccggccctgt tcgcctacga ggaagagctc gccgaggaag aggcaccccg atgaagaaga
      841 ccggcagcat gaaccacgtg cgcttcaccg tgagcgacat accacacgcg gagtccttct
      901 acgacccgct cctacgctac atgggctacg agctcgccga gaagagcgag gagcgtctgg
      961 cctggaagat gccctccccc gccggcaacc gacaatgggt gatcctgagc gcggcgtccg
     1021 aagaaggtcg tcgtaggaag cacgacaggc actcgccggg cgaccatcag ccgccccagg
     1081 ctttctccag tgcgtacgca atagcttcct cgaagcccag tcgccgccct tcagcccacg
     1141 ccgcatccca ccttgcctca gacaactgtg ctcgcgcggc cgccaagtag ggctcacgca
     1201 gtgcgtgctc tcttgtctcc tgccggtagc ccaccgtccc ccgtagggac tcggccgctc
     1261 cgaacagccg cgccacccgt tccgccgctt ctcctctggc gctggcagaa cacgctagcc
     1321 cctctaaggc ctccgcagcg acaagcttgt caccaagctg cccgctcagc gcgagacttt
     1381 ctcggtgcag ggcttcagcc tgctgtaggt tcccacgcat tagcgctgcc catcccaggt
     1441 tgtcgagggc gtattggaga tggcctctat ttccccgttc ccggtacaag cctgcagctt
     1501 cctcgtttag catggctcct cgctcatggt cgccttggag taagtactct gcgcccacac
     1561 tgatcaaggc ggaagcgagc agtgccttgt cgtctaactc cctggatagg gccaaccctt
     1621 cttcgtagaa cgtttcggct ctctcgtgat cgcctaggtc gctcgacacg ttcccaagtt
     1681 ggagcaggga cgatgcaaca ccccgctcgt cccccgcttc tcggctgagc gcgaggcttt
     1741 cctcgtagag tcgtgtcgcc cgctcgtgat ctccgcacac ataagcagct gaccctagta
     1801 tgcgtaggaa ggaagcctta tcactactct ggagcttggc tcgtgcgccg agcctcaggc
     1861 cctcttcggc cgctgccacc gcccgatcga tgtcaccttg aagatcggca agccagccta
     1921 caccctccag cgcctttacc cgcgccactg aggctctccc gtccttggcc agcgcctcct
     1981 cgagccatct cctaccctca ccgtagtatc ccctcatatc ccagaactgc cacagtgcac
     2041 ctgccaaccg catccccagt tcggcttcct ccgactgtag cgtccaggat agagccgccc
     2101 tcatgttgtc gtggtccatc tccaggcgct ctagccaagt cgcttcctcc ggtccctgca
     2161 attccgatgc gccctgttct gccagggcga ggaagtactc ggcgtgcaac ctccttatca
     2221 cctcagcgtt gccactgacc tccagcctct ctcgagcgta ctcacggatg gtctcgagca
     2281 tcacgaaacg cggctcctcc tcctctaccc cctcctcttg cctcaagagg ctcttgtcca
     2341 agagagcgga gagtctttcc aagacttcga cgaacacatc gccctcggcg tcgcatacgg
     2401 cctccacggc gtccagggca cacccaccag agaaaaccga caggctcgcg aagagtgcct
     2461 gctcgccttc gttgagcagg gcgtaactcc atgcgattgt gcccctcaga gtcctttgcc
     2521 tctctgggag attgcgggct ccaccagtaa ggaacttcag agggttgcca agcctagagg
     2581 acatcgcttg gggcgagagc aactttatcc tggctgcagc gagctctatt gccagaggca
     2641 gcccgtcgag gcgggcgcag atctcagcga ccgccggagc attctcattc gtcaaggaga
     2701 aatcggcatc tgcagcacga gcacgctctg caaagagtct tatcgcctcg taccctctaa
     2761 gcgtctcgaa aggcgacagg taacctggat ccggcagttc aagcggtctt accgcgtact
     2821 cgtgctcgcc gtagagtcga agtagagtac ggctggttac cagcaccttg aggttagggc
     2881 aaccagagag taactctcct accaaagggg cggcatcggc cacctgctcg aagttatcca
     2941 ggacaaggaa caactccttg ttgcttagat aatccctcaa ttcctccttg aggtctcgtc
     3001 cggcgctctc actcaccgat agagcccccg ccatggttga cgataccagc ttggggtcgc
     3061 tgataggagc cagcgccacg aagaataccc catcttcgaa ctcgtcgatc agctctgcgg
     3121 ccgactgcag cgcaagacgt gtctttcccg ttcccccggt tcccgtgaga gtgagcaagc
     3181 gtacgtcggc acgtctcagc aaatccgtga tctccgacac ctcgcgttcg cgtccgagaa
     3241 gcggcgtagg ttggatggga aggttgttca ggcgcacgtc cagggtcttg agcggcgaga
     3301 aggaagcggg cagatcggga gcagttagct ggaaaacgcg ctcgggcccg aagagatcct
     3361 tcagccgcct cacgcccagg tccctcagcc cagccccgac cggtaactgg tcgcgcacta
     3421 actcctgggt agtgagcgag agaagtattt gcccaccgtg cccggccgag aggagtctgg
     3481 ccacccggtt tatggtaggg ccaaagtagt ccccgtctcg ctcctcggca gttccagtgt
     3541 gcagggccat cctaaccttg aggggacccg tctcttccca ctcctcagag agtaagaact
     3601 tctgggcatc atgagctgcc tccacagctt ccggggcatt cggaaaggcg acgcaaaagg
     3661 cgtcgcccac cgtcttgaag acgaacccac cacggaactc tacagcctct cgtagcagct
     3721 cgtcgtggcg ggaaagagcc tgggacatgg cttcggggcc gcgttcccat agcctggtag
     3781 agccctctac gtcagtgaac aggaacgtga tcgtgcctgt tgggagccct gtcattgact
     3841 ttggtcctga tccacctggc gctctcattc cgacaaggaa gattatcgcc catccagcgt
     3901 cccatagcga gatcaagagt ggtaaggcta caggccctta cgggcacctc aacttctggc
     3961 ggttgccttc ggttactcag gctcacctct gttttaaact ccaatgttgg tcgttcagaa
     4021 gcctggcgtc agggcatagt aagagcgttg tgaggtgagg cctcatccat gggggccgct
     4081 ggctcctgcc gcactatcag gagtgttttg caggggtgag aggtcaatct cgacgtagtc
     4141 ggtgccatcc tcgtagcgca acctctcggg cagattcagg tcccttagca agtgaagcat
     4201 cctggtgttc tcggggagga cgatccccgt gaaggttctg acgcccctct tcagggccgc
     4261 ctctatcagg tgtctggtca gagcaagccc aagccctctc ccctgccacc tgtcctccac
     4321 ctccgccgcg tattctgccc gctcggtcgt cccctcgcga tcgaagctca ccacaccgat
     4381 gatctcttcg gggcgctcgg gatcgaccgc gaccagagcg aagcggttta tcccatcgac
     4441 gttcgtgaag taacctgctt ccctgtcgct tagctccggc tttgcggcga agaagcgcag
     4501 gtagatcgac cgcttgctca ggcggtggtg gaagcgctgg agtgctgagg cgttgtctgg
     4561 cgctatggca tggtaggcga tgctcgaacc atcgggcagc tcgacactac ccgagtcctc
     4621 ctcagcaagt ggagcaccag tcccatcgac caacagccct tctctggccg gctctttcat
     4681 tccaaaaccc cccaaactcg cgaagtcctc tgcaatacag agcttgccac tctttaccct
     4741 gaatgaacga attatctcat gactctcttg cgcctgacac aattgtagtc ccgcgcttcg
     4801 ggcaatcccc cggtgctata tcgccaggga cgccgccgag cagccagatg cgtaccccaa
     4861 ggcgctcaaa gcacgggcat ctcgcgtccc gcctcaccct gccgcttgcg ctgtaacgtc
     4921 atttctctag aattcccacc gtactaccgc cacgcctcca caacagaagg gagtgatcat
     4981 aagagatgat ggaccacgag agaacggaga aggaactcga ggagatcttc gaggagatcc
     5041 aacactacta caagacattc gcggacgacg ccctcgccct ccaggagcgc accctccagt
     5101 tggcccgaga gttgctggag atctgcgcgg gccgggaggc ttcaggcatg cgagacactc
     5161 tggagaacct ggccgaaagg agcaggggcg aacgcgagcg gttcgagagg ctggagagga
     5221 aggctggcga agcttacacg agggtactca agggccccgt cgatgagcat catcacaaga
     5281 ttgaggaggc cgaagcggat ctaaaggagg cgggttcttc ctaaccatct cgggtgagag
     5341 actcgacacc tgccttgccc tacttcggaa tcacttaagc gactgctgga agaagaagat
     5401 gttaccgggg tcgtattgcg ccttcacgcg cagcagccgg tcgtagttgg tcccatagta
     5461 ggcttgtgcc cagtcctcta ggtccgggtc ggggaagttg gggtacacgc gacctgaccc
     5521 ccacggacgc acagatgccc acgaccgcga aagccagcgc cgcgcggtct tcctctcgcc
     5581 cctcgacgcg tcggggtcga tgacgaccgc gtgcttgagc aagaagcgtt cgtcgcgatg
     5641 tacaaaggcg gtggcgtcgg cgctcacgcg attgtaggca ccgccccacg gcgtgaagtc
     5701 cagctcgcgc gactggcctg agatccgtcc ctcggtcagg ttctctacca gcgccgcgat
     5761 ggtctcggcg ggcagcggcc gaccgaagaa ctccgacttg ctgaacgttt gcccttgcgg
     5821 cgacggctcg ccctgcaagg tctccccgag ccggtcgtct ccttcggcca tggcgtcgcc
     5881 gagcttagcc aggtagcgct tggtctcccg caaggacgcg tgctcgtagg aagccgaggt
     5941 cgggtcagcg cccgcgctgg agaccagctc gtcgagcagt tgtgcggtgt catcttcggt
     6001 gccgagcatg gagccgaaca cgttgacaac gggcggcctc tcgggacacg ttgacaacgg
     6061 gcggcctctc ggggtcgcgt gaggcggtga cgagcaggct ggcggcgagc tcatcgggcg
     6121 ccgcgggagc ccatgcctgc caggcgctga tgagtgcggc tgcgtgggtg tgcggccaga
     6181 gaaggtggaa gcccgttgcg gccggcgccg gcagagtgcc gaagatcaag gacgtgacca
     6241 cgccgaaatt gccgccgccg gcaccgcgca gcgcccagaa cagctcctcg tcgtgctgct
     6301 cgtcgcacac tacgacgcga ccatccgcca gaactatctg taatccgagc aggtggtccg
     6361 aggtcagacc gtgtttgcgg ccgaggatcc cgagtccgcc gccgagcgcg aggcctgaga
     6421 ttccaaccga ggggccgcag cctcccggga tggtaaggcc gtactgggcc agcgtgtcgt
     6481 acacctcgcc gagccgggct cctgcgccca ccgtcgccac gccgtccgag acactcacgg
     6541 agcgcatcgg cgtgacgtcg atgacgatcc cctccgtcga tgagcgtccg gcgaagcagt
     6601 gccctccact gcgagcagca gtccgcgaac cggactccct agcgaacgag atcgtctcag
     6661 agacgtcctg tggggttctg caaagcacga cggcctgggg ccttatatcg tggaagttgg
     6721 cgatggccgg cttgcgtgtc gactcgtagt cgggcgagcc agggaggacg acgtcaccag
     6781 agacagcgcg ttgcagcgcc tcccaatcgg gagccgaggg caccatgtct cgacgacccc
     6841 cgctacgcac caggacccac cttgctcacg gagaacgcgc cgccctggga atcgcgcgcg
     6901 aggttcttgg ctttgcggga ggttcggctt gcgacgaagt gcaacgcatc atccagccag
     6961 acggccagca ctggcacaag gtgtgggcgg ccgttagaac gcaccgtcgc cagccagtac
     7021 gcatcagcct ccgcgaggcg cctgcgcgct tccgcccacg atgtcgacct cacatcagcg
     7081 gcaaggtatg actgcgcaat cacggttcct cttctggaag attcatgata tctcctttgc
     7141 tatggatgtg acctatgctc tcgctagtcc acgacttcgc tgcaggtgat gtcggcgaag
     7201 gtagcttcta tgtaatggtc gacgctgcat cgctctccgg ttgcgtgctt ggccttatgt
     7261 tctggttgag gcggaagacg ttcgtcggat cgtagcgacc cttgatccca gcgagccgct
     7321 cccaggtaga tcctggatag gcctcacgga cgcgcccttc gccctcctca cccaggaagc
     7381 cgacatacgc accatcctca cgctgtctca gggccgccgc gaagtcgctg acccaggcct
     7441 cgcgcgcgac ccggtcctca gggccctcgt agaacgcggc gacgttgacc atgatgcgcg
     7501 aggagcggtg ggcgaaggcg gtggcctctg cgggcacgcg ggccatggca ccgccgagca
     7561 cccgtagctg ggccactcgc attaccgcgt cggaggcgag caggtactcg acgatcatct
     7621 ctgcaacgtc gcggtcgatc ttgtccacga acatggtgcg cgccgccgcg gtcgggtggt
     7681 agtcgccctc ctcgggaggg tagatctcag ggtatggcat cggcctcacc atgtcggcta
     7741 tcggctcggc gagggcacga aacggcgcga tggcgcgctc accggcctcc acctcgcccg
     7801 cgtagaccag catggccatg atgatgagct ggccgtgcgc ctcggctggc agaaatggca
     7861 taggcggcgc gaccatcacg ttggcgatgg tcgagagctc ctcaggagct gcctccgcct
     7921 cggcgatgaa cgaagcgatg acctcgggtg tggccgggag caccagcatc ccacctacga
     7981 tcgtgctcac ctcgtgcagc ctgtagtgat atctggtcac aaccccgaag ttgcctcctc
     8041 cgcccctgat cgcccagaag aggtcgggat gactctctgc atccactcta aggagctctc
     8101 cgtctgcggt cacgatctcg gcggccagca catcgtcgat tgtgaggcca tgcttgcgca
     8161 ccaggtaccc gaccccgccg cctaaagtga cacctccgat acctaccgag ccggtgtcac
     8221 caaagcccgt ggcgaggccg tgagctccca cagcggcggt gtactcgcca gcagtcaacc
     8281 ccgtctcggc ccaggctgtg cgcccttcgg tgtcgatctc aagattcttc atctccgaga
     8341 ggtcgagcac tatgccccct tcggtagtgc tgtgaccggc gacgctgtgg ccgccgctgc
     8401 ggatggcgag ctccaaccct ctctcgcgtg ccagcgtgat gacgtgcgag acgtcggtcg
     8461 gatcagcgac ccgaacgatg agcgctgggt ggcggtcgaa gccaccgtag aagacggtgc
     8521 gtgcctcgtc gtagccgtcg tcctgggggc gtataagctg gcctcgcacc ttcgacgaaa
     8581 acgcccggat taccgcctca tcgagcggct tgatcgggga caacctcttg gtgtcttgcc
     8641 gattgtcagt cataatctta tctccgatcc ttggtttgct tctggtaaga aatatactgg
     8701 ctgccgggat ttaggcatcc accgaaagtt ggagcgaggg ttggaaatcc tctcggtccg
     8761 actgacacgt gcgctgcggt cgggacaccg caggaggtaa gtagtctcaa atgtcacagc
     8821 cctccggatg gaatcgagtt ccattctgca tctg
//
LOCUS       DASTKM010000220         5966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1301344, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000220 DASTKM010000000
VERSION     DASTKM010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301344"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..400
                     /locus_tag="VFQ10_09090"
     CDS             152..400
                     /locus_tag="VFQ10_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965535.1"
                     /translation="MSQDVSTAQIVTAITTVFYAAVFAIGYYMMIRGNREVLQEMREE
                     RLSGGRPQVIVETSLLNLSLVEFVVPHVSGGSSEGYNL"
     gene            complement(537..902)
                     /locus_tag="VFQ10_09095"
     CDS             complement(537..902)
                     /locus_tag="VFQ10_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9965536.1"
                     /translation="MTTDHRRVDAELVQEVLLCERVVQQILEAVMTEHIGGAAPYERL
                     EGRTGHRNGHKPRMLRTRVGTLNLVVPQDREGTFSTRKVKDVTEALCGDSPLRCVSCS
                     CKVFVVHLHRNTFVKSFLA"
     gene            1090..1650
                     /locus_tag="VFQ10_09100"
     CDS             1090..1650
                     /locus_tag="VFQ10_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965537.1"
                     /translation="MANANIDKAGLLWGCLWRASLYELLLGAAFAGFYGAVFGTLAFP
                     VVGTLIGFVFGAPAGALVGLPLGLIDGLLLMIWLTVSTHEDEILNPHRYRLRAGAVCA
                     VGSLIALLGDWALHGLPDSNYFATSRTACLSVYLIDPPCALDEGMPIAMDLTILVIVP
                     LMMAVLASWFTGRIVAGWYAGAVSET"
     gene            2113..3462
                     /locus_tag="VFQ10_09105"
     CDS             2113..3462
                     /locus_tag="VFQ10_09105"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9965538.1"
                     /translation="MSEYSPTWCTELYGILIHPSELRILMLWGAAGWSLPQMSLDQAV
                     WEADVGKVSEAMRLALGAEVTTLRYASYSVSVDAHQIDAIYVLESHNPAWEPQDGAEW
                     VGRAALADLPLARPEHRTLVAAHLAEAESGQIPEHRPPWARVGWFEQAAAWTQSELAR
                     LGYSLTAPVEQIKSWGISCILRAHTASGDVYFKAASSLPLFAHEPAVMRGLAGLYPDH
                     IPAPLSIDEQHRWMLLADFGAPIEWDAPVEVQEEMLRLYGEVQRDAATRVGDLLAIGC
                     LDRRLERLAAQVDPLLEDTATLASQLDAVEIERLRALGPQLRAMCAELASYAVPHTLV
                     HGDLHLGNVARSAGSYLFFDWTDACVAHPFFDTISIFQVKDPNVQARLRNSYLGVWAA
                     HEPIECLLEAWKLAKPLCALHQAVSYQHIVATLEDTSKPELASGLPYWLRTLLQSLE"
     gene            complement(3513..3749)
                     /locus_tag="VFQ10_09110"
     CDS             complement(3513..3749)
                     /locus_tag="VFQ10_09110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9965539.1"
                     /translation="MEAELISSCDLPPLKDRADTLRRCGETFYGYFVEVRLAGAISYK
                     SAHGIMNVYRLMVRPDHFRKGIARCLLGHVERGL"
     gene            4739..>5966
                     /locus_tag="VFQ10_09115"
     CDS             4739..>5966
                     /locus_tag="VFQ10_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET9965540.1"
                     /translation="MAFTRKDFLKLGLVGGAGLALSSGASASSPRGRVRTTGHLLRSK
                     AQLPKPFRIPLIVPPVLKPVRTDSWARWARAPTDYYEITHKEGKTEILPGLTTALWGY
                     DGIFPGPTIESRSGRKIVVRYYNELPVPVTTHLHGGRTPPGSDGYPTDLILPRSSSSH
                     THHEHLAHAAWSFHRGSKDYVYPLEQRAATLWYHDHRMDFTGPQIWGGLAGFHIVHDD
                     EEDELPLPKDEKDVPLMLCDRAFGEGGSFLYPSLDSSLQGEPGVKAHYMEGVLGDAIL
                     VNGVPWPFMEVSNTRYRFRILNASNARRYRLALDPGPSDGLPFVQVGSDGGLLGAPIS
                     HRTIPIAPAERFDVVIDFSKYPVGTKVTLKNRLGLGTTSRVMRFHIVRKEKERSAVPE
                     RLSDMGEFKALGESNAR"
BASE COUNT         1161 a         1799 c         1824 g         1182 t
ORIGIN      
        1 ctcgccgacc gacccaaagc ccgcctacta cacgtcgagg acgccttctg agctccactg
       61 tatgttgtgc acgtagcagt tcctatactt cggggtgacg ttttatcggg cggcaatgga
      121 aatcgacagt tgcgacgcca aaaggagtag cgtgtctcag gatgtgtcta cagctcagat
      181 agttactgcg ataaccaccg tgttctacgc ggccgtcttc gcgatcgggt actacatgat
      241 gataaggggt aaccgcgagg tgctacaaga gatgcgcgag gaacggctct ccggtggacg
      301 cccgcaagtc atcgtcgaga cgagcttgct caacctttcg ctagtggagt tcgttgtgcc
      361 tcacgtaagt ggggggagca gcgaaggata taacctttga aatttctgac cccatagagg
      421 attcttctgg ctatgtcatc tcggatttgc cttaccttaa ggagggcatt cctttgctcg
      481 gatcgggtga gagaatacgc tgcctctggg atcacatcga ccgtctcatg ccctctctac
      541 gcgagaaagg acttcacgaa ggtattgcgg tgaaggtgaa ctacaaagac cttacaggag
      601 cagcttacac aacggagtgg agaatcgccg caaagtgctt cggtcacgtc cttaaccttt
      661 ctggttgaga atgtcccttc cctatcctgg ggcactacaa gattcagggt gcccaccctg
      721 gtgcgtaaca tcctcggctt gtggccgttg cggtgcccgg tgcgtccctc gaggcgctcg
      781 tagggtgctg ctcctcctat gtgctcggtc attacggctt ccaggatctg ctgcacgact
      841 cgctcgcaga gcagaacctc ttgcaccaat tcagcgtcta ccctacgatg atcagtggtc
      901 atcgtcactc cctttcgatt ggttgacttt agtcaccaac cctcttggaa gacgatggca
      961 tctgtttcta gcgggccatc aaattacaga actttaaggg cacaacccgc cagtggttcg
     1021 gggcagagcc ggccatcgct gtccctgttc acgccacgcc gggagaagtg gcatcctgag
     1081 aagttaggta tggcgaacgc gaacatagac aaggccgggt tgctctgggg ttgcctttgg
     1141 cgggcaagcc tctacgagtt gctgctcggt gccgccttcg ccgggttcta cggagccgtg
     1201 ttcgggaccc ttgcctttcc cgtcgtgggt acgttgatcg gcttcgtctt cggggcaccc
     1261 gcgggcgccc tggtggggct gcccctcggc ctgatcgatg gcctcttgct gatgatctgg
     1321 ctcaccgtta gcacccacga ggacgagatc ctgaacccgc atcgctaccg actacgggct
     1381 ggggccgtgt gtgcggtcgg aagcctcatc gcgctattgg gcgactgggc gctgcacggc
     1441 ttgcccgact ccaactactt cgccacgagt cgtacggctt gcctatccgt ctacctgata
     1501 gatccgcctt gcgcactaga cgagggcatg ccgatcgcca tggatctgac gatattggtg
     1561 atcgtgccct tgatgatggc ggtgctcgcc tcgtggttta cgggtaggat cgtcgccgga
     1621 tggtacgcgg gggcagtctc cgaaacgtga cccgatccgc gcctgtcttc gagtctgcac
     1681 cgagcgccgc gaagcattcg ttcggcagtc gttttcgctg cgatggcaag cttccctcag
     1741 gctggatctc gccggctacg gtctcacggc ttgcctctat tcacaggagt aagtggaaaa
     1801 gcagaattct gcgaagtccg aacacagaac cctgctaaac tacccctatc ggaccggggg
     1861 cggagcagca ttcccccgcg caggtaccaa accctaaaac cacacatgtt gtggtgctgc
     1921 ataaaaatgc gcgatcttgc atgtggtcat tgtgggttca ctacagttga tatgatggtg
     1981 ttaaggatcg tagagatgcg tggtgggggg caccgtggaa caggataagc aggctcggtg
     2041 gtgattccga gggaagacgg tctgggacag tctaactatc cactgcttca atactgccat
     2101 tgaggattac acatgagcga atattcacct acctggtgca cagagctcta cggcatcctg
     2161 atccacccca gcgagctacg gatcttaatg ctgtggggag cagccgggtg gtccctgccg
     2221 caaatgtcgc tcgaccaggc ggtgtgggag gccgatgttg gcaaggtcag cgaggcgatg
     2281 cgactagcac tcggcgccga agtcaccacg ctgcgctacg ccagctacag cgtgagtgtg
     2341 gatgcgcacc agattgacgc tatctatgtg ctagagagcc ataacccggc gtgggagccg
     2401 caggacggcg cagagtgggt tgggcgcgcg gcattggccg acctaccgct cgcgcgcccc
     2461 gagcaccgga cgctcgttgc ggcgcatctg gccgaggctg agagcggaca gatcccggag
     2521 catcgcccac cctgggcgcg cgtcggctgg tttgagcagg ccgcggcgtg gacgcagtcg
     2581 gagttggctc ggctgggcta cagcctgacc gctcctgtcg agcagatcaa gagctggggg
     2641 atctcgtgta tcctacgcgc ccacactgcc tctggtgatg tctacttcaa ggccgcgtcc
     2701 agcctgcctc tgttcgcgca cgagccggcg gtgatgcgcg gcctggccgg gctctacccc
     2761 gaccacatcc ccgcgccgct gagcatcgac gagcagcatc gctggatgct gctggcggac
     2821 ttcggtgcgc cgatcgagtg ggacgccccg gtcgaagtgc aggaggagat gctgcgtcta
     2881 tacggcgagg tgcagcgcga cgcggcgacg cgggtgggcg acctgctggc gatagggtgc
     2941 ctggaccgcc gcctcgaacg gctcgcagcg caggtcgacc cgttgctgga ggacacagcg
     3001 acgcttgcca gccaactgga tgcggtggag atcgagcgac tccgcgcgct cgggccgcag
     3061 ctgcgggcga tgtgtgccga gctggcgagc tacgccgtgc cacacactct ggtgcatggc
     3121 gacctccatc taggcaacgt cgcacgctcc gcagggagct acctattctt cgattggaca
     3181 gacgcctgtg tagcgcaccc atttttcgac acgatcagca tctttcaggt caaagatccc
     3241 aacgtgcagg cgaggctgcg caacagctat ttgggcgtgt gggcagccca cgagccgatc
     3301 gagtgcctgc tcgaagcttg gaagttggca aagccgctgt gtgccctgca ccaagcagtc
     3361 agctaccagc acatcgtcgc aaccttggag gacacttcta aacctgaatt ggcctcgggg
     3421 ctaccttact ggctgcgcac gctcctgcaa tcactggagt agctaactac tggcggaagg
     3481 gtcgatcgcc ccgatcgcgc cggaagtggc ggttacaacc ccctctctac gtgccccaat
     3541 aagcaccggg cgattccctt gcgaaagtgg tcgggacgga ccataagcct gtagacgttc
     3601 atgatgccgt gcgctgattt gtacgagatg gcccccgcca accgcacttc cacaaagtag
     3661 ccgtagaaag tttcaccgca ccgcctcagg gtatcggccc tgtccttcaa cggcggcaaa
     3721 tcgcaggagc ttatgagttc ggcctccacg gcgtccaagg ccccttgcag tcttccagtt
     3781 cttgggtgag gccgagctgg aacagagcca ccaatctcac ggaggccgac ctgacttatg
     3841 ccatggggtt aaccaacgag gagctacagc aacaagccgc ctctcccgaa ggcgccacca
     3901 tgcccgacgg ccagaaatac gaagaatggg tcgagagcaa gggccgcgga cagtaggaga
     3961 agaagggcag acgttcgtta cggacttcag aacgaacttc gcaagggtcg cttcggaagc
     4021 ttatttgcag aggactgcgt agcaccgaga cttagggccc cgtatcaggg ctaaacctag
     4081 cagcgccctc agggacgcga cgttgtcgcc tgagcgccat cgggagcggc aagtgcggag
     4141 agaactagta gctgggcctg cgggtaatcc tcacgccctg gtgggctatc cgcaggggct
     4201 tctggttcgg gcacgcgctg ccccggcggg ctctgccttg tcacgtagta tccgaatgca
     4261 acggatctgc gaacgcacct gtgaggtgcg gctctccgct gcggttggcc aggagccgga
     4321 ggtagggctg acgtgaacac cgtgcccacg agcttctcgg cagattacgt ctgccctgtg
     4381 aaggatcatc gtgtggcggc tgggccctcc cgagagcaaa gtgaggtccc cgcatgcttc
     4441 ggggcatgta gccattattc accggaatgc gtcgaagacg tgttctggca attgcgtatg
     4501 tacgaagttc tatgaagttc gatgataaag aatcgaaatc tagctcattt gcgtgatgcg
     4561 tatcctttgc gcaaggagta cgctgcaatc attgcgcggc taagagaccg ccgtgtgaag
     4621 gcgcaggaca gctacctgtc gtcctcttag accagggcta gctaccctac cgacgacctg
     4681 ccgttagata ggaacgcctc cccagaagcc gggttggtaa aggaagagga caagttacat
     4741 ggcattcacg cgcaaagatt tcttgaagct agggctcgtg ggtggagcgg gacttgcgct
     4801 ctcgtccggc gcctccgcct cttcgcctcg ggggagggtc aggacgacgg ggcatctgct
     4861 gcgcagcaag gcacaattgc cgaagccatt taggataccg ctaatcgtgc cccctgtcct
     4921 gaagccggtg cgcacagatt cttgggcccg ttgggcgcga gcccccacgg actattacga
     4981 gatcacgcac aaggaaggaa agaccgagat cctacccggg ctaacgacgg cgctctgggg
     5041 ctacgacggg atcttccccg gccccaccat cgagagccgc agcggtcgga agatcgttgt
     5101 gcgctactac aacgagctgc cggtcccggt cacgacgcac ctgcacggcg ggaggacgcc
     5161 acccgggagc gacggctacc ccaccgacct aattctcccg aggagtagct cctcccacac
     5221 ccaccatgag caccttgccc atgcggcttg gtcgtttcac cgagggagta aggactatgt
     5281 ctacccgctg gagcagcgcg ccgcgaccct gtggtaccac gaccaccgca tggactttac
     5341 cggtccgcag atctgggggg gtctggccgg tttccacatc gtccacgacg acgaggagga
     5401 cgagctgccg ctcccgaagg acgaaaagga cgttccgctc atgctctgcg accgcgcctt
     5461 cggcgagggt ggctccttcc tctacccttc cctcgactcc tcgctacagg gtgaaccggg
     5521 cgtgaaggcc cactacatgg aaggcgtcct cggagatgcc atcctcgtca acggcgttcc
     5581 ctggcccttt atggaggttt ccaacacccg ctaccgcttc cggatcctca acgcctcgaa
     5641 cgcccgccgc tacaggctag cgctcgatcc tgggccgtcc gacggtctcc cttttgtgca
     5701 ggtaggcagc gacgggggac tacttggggc tcccatctcc caccgcacga tccccatcgc
     5761 cccggcagag cgcttcgacg tggtgatcga cttctccaaa tacccagtcg gaacgaaggt
     5821 cacgctgaag aaccgcctcg gcttgggtac cacctcacgg gttatgcgct tccacatcgt
     5881 ccgcaaagag aaggaacgga gcgcggtgcc cgagcggctc tcggacatgg gtgagttcaa
     5941 ggcgcttggc gagtcaaacg ctcggc
//
LOCUS       DASTKM010000221         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_67045, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000221 DASTKM010000000
VERSION     DASTKM010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67045"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFQ10_09120"
     CDS             <1..417
                     /locus_tag="VFQ10_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HET9965541.1"
                     /translation="ELEIEDCVSEMMLARGRHLRSKVSGTLRDGVTDATLLDTMHPTP
                     AVGGHPRSEALEQIHALEPFDRGWYAGPVGWIGAEDSEFAVGIRSGLVRGRTLALFSG
                     AGIVAGSVPDEEWAEIEQKIGDFTGMFGLEQGHAAS"
     gene            392..>2075
                     /gene="menD"
                     /locus_tag="VFQ10_09125"
     CDS             392..>2075
                     /gene="menD"
                     /locus_tag="VFQ10_09125"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HET9965542.1"
                     /translation="MNRDTPRANRLWAYLIVEELLRCGVDFFCVAPGSRSTPLVAALA
                     ANERARSLIHFDERGTAFAALGHARATGRPAAWITTSGTAVANGLPAVVEAATDGVPM
                     VLLTADRPPELRQTGANQTIDQSDIFGDYVRWRFDLPAPDPGIDPTMILTTVDQATYR
                     ASRAPQGPVHLNLMFREPLLPDPGDEPVLSGPSSWAQSDEPYTRYAATKPAVDEMEIY
                     RLWETLRPVKQGLVVAGRLASRKQGEAVLRLADTLGWPLLPDIGSQVRLGVVGLAAHY
                     DALLAGDSFAGAHAPEAVVHVGGRALSKRLEQFLAHSRPDPYVVVRENPFRLDPAHSV
                     THSVEANVLDFCSAMVRVATEDPPAMDSSWTAGWLEASEEVARQLDGISSGEPNEPLV
                     ARVLSWNVPPDHGLVVASSMPVRDLDTYAATDGAPVPVAANRGASGIDGTVATAAGFA
                     RGLGRPVTLLIGDLALLHDLNSLAMLRDVQVIVVVLNNDGGGIFSFLPVARHEEFFEA
                     YVGTPQHVGFEPAAKMFGLAYEHPGTIEDFVEAYGSACSRGSSTLIEIDTDRK"
BASE COUNT          355 a          708 c          654 g          358 t
ORIGIN      
        1 gagctggaga tagaggattg tgtctctgag atgatgctgg cgcgggggag gcacctcagg
       61 tcgaaggtaa gcggaacgct cagggatggc gtgacggacg ccacactgct cgacacaatg
      121 catcctacgc cagccgtcgg cggtcatccg cgaagcgagg cgctggagca gatacacgcc
      181 ctcgaaccct tcgaccgggg ctggtacgct ggccctgtcg gttggatcgg ggccgaagac
      241 tccgagttcg ccgttggtat ccgctcgggg ctggtgcggg gaaggacgct agccctcttt
      301 tcgggggccg ggatcgtcgc cggctccgta cctgacgagg agtgggccga gatcgagcag
      361 aagatcggcg acttcacagg gatgttcggg cttgaacagg gacacgccgc gagctaatcg
      421 cctctgggcg tacctgatcg tcgaagagtt actccgctgt ggcgtagact tcttctgcgt
      481 cgcccctggc tcgcgctcga cgcccctggt cgcggcgctc gccgcgaacg aaagggctcg
      541 tagcctcatc cacttcgacg agcgtgggac cgctttcgcc gcgcttggtc acgcgcgcgc
      601 cacaggtcgt cctgcggcct ggatcaccac ctcgggtacc gcggtcgcca acggtcttcc
      661 tgctgtcgtg gaggccgcga ccgacggcgt gcccatggtc ctgcttacgg ccgaccgtcc
      721 tcccgaactc cgccagaccg gcgccaacca gaccatagac cagtctgaca tcttcggcga
      781 ttacgttcga tggcgcttcg atctgccggc ccccgacccc ggcatcgacc caaccatgat
      841 cctgaccact gtggatcagg ccacctaccg cgcgagccga gcgccacagg gccccgtcca
      901 cctgaacctg atgttccgcg agcctctact ccccgatcca ggtgacgaac ccgtactttc
      961 cggaccatcc tcatgggcgc agagcgacga accgtacacc cgctacgccg caacgaaacc
     1021 cgctgtggac gagatggaga tctaccgcct ctgggaaacg cttcgccccg tgaaacaggg
     1081 tctcgtcgtg gccggtaggc tggcctcccg caagcaaggc gaggcggttc tgcggctcgc
     1141 cgatactcta ggctggccgc tcctgcctga tataggctct caggtgaggc tcggcgtagt
     1201 gggcctcgcc gcccactacg acgcgcttct agccggggac tcattcgcgg gggcacacgc
     1261 gcccgaggct gtagtccacg tcggaggaag ggctctttcc aagcgcctgg agcagttcct
     1321 cgcccacagc cgcccagacc cttacgtcgt cgtacgcgag aacccattca ggctcgaccc
     1381 ggcccacagc gttacccaca gcgtcgaggc gaacgtcctt gacttctgct ccgcgatggt
     1441 acgggtcgct acagaagacc caccggcgat ggactcttca tggacggctg gctggctgga
     1501 ggcatcggag gaggtggctc gccagctgga tgggatctcc tccggagaac cgaacgagcc
     1561 cctcgtggcc cgggtcctat cgtggaacgt cccccccgac cacggtctcg tcgtcgcgag
     1621 cagcatgccc gtgcgggacc tggataccta cgcggcaacc gacggcgcac ccgtccccgt
     1681 cgccgccaac aggggcgcga gcggcatcga cggcaccgtc gccacggcag caggtttcgc
     1741 cagaggcctg ggacggccag tcacgctcct catcggggac ctcgcgctgt tgcacgacct
     1801 gaactccctc gccatgctgc gggacgtaca agtcatcgtc gtggtgctga acaatgacgg
     1861 tggcggtatc ttctccttcc tacccgtagc caggcacgag gagttcttcg aggcttacgt
     1921 cggtacgccg cagcacgtag gcttcgagcc agcggcgaag atgttcggcc tcgcttacga
     1981 gcatcccggc acgatcgagg acttcgtcga agcgtacggc tccgcgtgtt cccgcggctc
     2041 ctcgactctg atcgaaatag ataccgaccg caagg
//
LOCUS       DASTKM010000222         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1478066, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000222 DASTKM010000000
VERSION     DASTKM010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478066"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFQ10_09130"
     CDS             <1..232
                     /locus_tag="VFQ10_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HET9965543.1"
                     /translation="SVGWPVHRMSAVDRSILRLALYEMLFVDDVPPEVAVNEAVELAR
                     GFSSDEAPSFVGGVLRGAELKAQGKVGHGGRG"
     gene            216..1124
                     /locus_tag="VFQ10_09135"
     CDS             216..1124
                     /locus_tag="VFQ10_09135"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET9965544.1"
                     /translation="MAGGASGKYGQIRWDAHRRAFEDYLHGLRFTVEPRLRGLEEAMR
                     YSMLAGGKRVRPTLCMETAWIFGAEPSHVLPSAAAIELIHTYSLVHDDLPAMDDDDYR
                     RGRPTAHKKFGEAMAILAGDAFFGEALTLITVHQKGSCKEILEVVRELADSTGVNGMV
                     GGQVLDMNQTGLAEATEPETLYMIHEYKTGALIKSSARIGAILAGATGEELAAVSEYA
                     SQLGLCFQIVDDVLNATSTREELGKSAGSDAEQGKATFAGVFGLEGARREADEAAKRA
                     LMALSKVDGDTAGLQELAYFVRHRES"
     gene            1133..1936
                     /locus_tag="VFQ10_09140"
     CDS             1133..1936
                     /locus_tag="VFQ10_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET9965545.1"
                     /translation="MESNPKGGPKRLDTLLVERGLAASRSSAQGLILSGSVRVGDAVV
                     TKAGSRIGADQPLYVDHAERFVSRAGQKLDAAIGEFGVLVEGRSCFDAGASTGGFTDV
                     LLRRGAERVLAIDVGYGQFDWTLRNDPRVTVMERTNVRRLAGENLPFQPDLLVADLSF
                     ISLTVALESILSTTPALREAVLLVKPQFEAGPESVGRGGVVKDREVHTAVIRSVARYF
                     GTLGFGAIGVTRGAVAGRKSGNLEYPVHLLRGVEPGLDEVRIREVVGGG"
     gene            1929..2738
                     /locus_tag="VFQ10_09145"
     CDS             1929..2738
                     /locus_tag="VFQ10_09145"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET9965546.1"
                     /translation="MGDTLDAARIRRVGIVANPRVSDGYVDRLVAVLEKNGVEISEVR
                     GDDQPDGATDIDLVFVLGGDGTMLRASRMYQGRVLLGVNLGKVGFMSGMLPEELESGV
                     EKVLGDGLEVQEYRMLEVRVGNEEPGLAANDAVLIKKRPNQLISVDVSIGGEELFAFR
                     CDGFIAAPPLGSTAYALAAGGPIISGDAGCYVLVPIAPHALVSRPLVLGEDQVAELEL
                     VEREALLSLDGAEPREMPPGGRVEVRLSSASVRIGRTEDWSWWRAVRRTFL"
     gene            2708..4405
                     /gene="recN"
                     /locus_tag="VFQ10_09150"
     CDS             2708..4405
                     /gene="recN"
                     /locus_tag="VFQ10_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET9965547.1"
                     /translation="MASSSEDVPVVLTELSVENVALIEEATLELSPGLNVITGETGAG
                     KTLLATALQMLLGGRARSETVRSGARRATVEGTFVLPEETGSRFLNEALGDLAEEVED
                     DDGLVLRRTLTGEGRSRCYVGGVSVSVKALASLGERLVSYHSQREQTRLTEPADQLAI
                     LDDFLDPAAAEARHEHELIWRAVENDRAALQEIRTTSEARLREVEFLRYQVSELESAG
                     YDQQELLDLSRERDRLRNVTDLLQTTSAASAGLSSEDGTGAVDAVARAASELETASGY
                     DDSLSGLLKRLHGISAELDDVLYELRSYLEELEADPNRLEAVEDRLAAMRALERKYGD
                     VEGYLQESRERLSRLENLDEETAGLEERLAEGERRLEVIAAELRSGREAAAQRLSGKV
                     QENLADLNLGKTTFRAELVPAEPGPSGTERVEFMIQPNPGEPELPVRRYASGGELSRI
                     MLAIRLAQERVEPGATYVFDEVDAGIGGETATAVGAKLRELGDRNQVLTITHLPQIAS
                     GAGTHVIVEKEEAGGRTITRIERIEGEERRRELARMLSGRIDEASLAHASELLVSGR"
     gene            4477..>4701
                     /locus_tag="VFQ10_09155"
     CDS             4477..>4701
                     /locus_tag="VFQ10_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9965548.1"
                     /translation="MGIAGDIALILVAALLGGILARWLGLPLILGYIAAGVLIGPNTF
                     GPTVGDVHEIELLAEIGVALLLFTIGLHFSL"
BASE COUNT          880 a         1414 c         1621 g          786 t
ORIGIN      
        1 ctcggtaggg tggccggtgc accgcatgag cgcggtggac cgttcgatcc ttcgcctcgc
       61 gctctacgag atgctcttcg tggacgacgt cccccccgaa gtcgcggtaa acgaagccgt
      121 tgagctggca aggggattct ccagtgacga agcgccctcc ttcgtagggg gagtcttacg
      181 aggcgctgag ctaaaagcgc agggcaaggt tgggcatggc gggcggggct agcggcaagt
      241 acggccagat caggtgggac gcgcacaggc gcgccttcga ggattacttg cacggactca
      301 ggttcacggt agagccgcga ctcagaggac tcgaagaagc catgaggtac tctatgctcg
      361 ccggcggaaa gcgtgtgagg cccacgttgt gcatggagac tgcctggatc ttcggggccg
      421 aaccttccca cgtgctccct tcagcggcgg ccatcgagct catccacact tactcgcttg
      481 tccacgacga ccttcccgcg atggacgatg acgactacag gaggggaagg ccgacagccc
      541 acaaaaagtt cggcgaggcg atggccatac tcgcaggcga cgccttcttc ggcgaggccc
      601 tgaccctgat cacagtccac cagaaaggga gctgcaagga gatcctcgaa gtggtgcggg
      661 agctcgcgga ctcgacgggg gttaacggga tggtcggagg tcaggttctt gacatgaacc
      721 agactggcct tgccgaggcg accgagcccg agaccctgta catgatccac gagtacaaga
      781 cgggcgccct gatcaaatcc tcagcccgca tcggggccat actggcaggg gccactggcg
      841 aggaactggc cgctgtctcg gagtacgcct cgcaactcgg cctgtgcttc cagatagtcg
      901 acgacgttct caacgccact tcgacgcggg aggagctcgg caagagcgcc ggttccgacg
      961 ccgagcaggg caaggccacg ttcgcaggcg tcttcggcct cgaaggtgca agaagagagg
     1021 ctgacgaggc cgcaaagaga gccctcatgg cgctctccaa ggtcgacgga gacaccgcgg
     1081 gtctgcaaga gctggcgtac ttcgtgcgcc acagggagag ttagccccag gcatggagag
     1141 taacccgaaa gggggcccca agcgattgga caccttgctc gtcgagcgag gcctcgccgc
     1201 gagcaggagc agcgctcagg gtctcatcct ctcaggcagt gtacgcgtgg gcgacgcggt
     1261 agtgaccaaa gcaggctcgc gcatcggcgc tgatcagccc ctgtacgtcg accacgcaga
     1321 acgtttcgta tcacgggccg gccagaaact ggacgccgcc atcggcgagt tcggcgtgct
     1381 ggtggaggga cgttcctgct ttgatgccgg agcatcgacg ggagggttta ccgacgtact
     1441 ccttcgacga ggagccgaga gggtgctcgc tatcgacgta ggctacggcc agttcgactg
     1501 gacgctgcgt aacgatccac gcgttaccgt catggagcgg accaacgttc gtcgattggc
     1561 cggagaaaat ctgccgttcc agcccgacct tctggtggct gacctctcgt tcatctcgct
     1621 cacggtcgcg ctggaaagca tactctcgac cactcccgcg cttcgggagg cggtcttgct
     1681 ggtcaagccc cagttcgagg cgggacccga gtcggtagga cgcggaggcg tggtgaaaga
     1741 cagggaggtg cacaccgccg tgatacgcag tgtcgcccgg tacttcggta ccctcggttt
     1801 cggtgctatc ggtgtgacac ggggggctgt cgcaggaagg aagtcgggga atcttgagta
     1861 cccggtgcac cttctcaggg gagtggagcc gggcctggac gaggtgcgaa tcagagaggt
     1921 ggtcggcggt gggtgacacc ctggatgccg cccgaatccg cagggtaggt atcgtcgcca
     1981 accccagggt gagcgacggg tacgtcgacc ggcttgtggc cgtcctcgag aagaacgggg
     2041 tcgagatctc cgaggtcagg ggcgacgacc agccggatgg tgccacggac atagacctcg
     2101 tcttcgtgct cggcggcgac gggactatgc tgcgggcatc gaggatgtac caggggcggg
     2161 tcctcctcgg ggtgaacctt ggtaaggtcg gtttcatgtc ggggatgctc cccgaagagc
     2221 tcgagtcagg cgtcgagaag gttctcggag acgggctcga agtccaggaa tacaggatgc
     2281 tcgaagtgcg cgtcggtaac gaggagcctg gcctcgcggc caacgacgcc gtgctcatca
     2341 agaagcggcc caaccagctc atctccgtcg acgtctccat cggcggggag gagttgttcg
     2401 ccttccgctg cgacggattt attgcggcgc cccccctcgg ctcgacggcc tacgcgctcg
     2461 ctgcgggggg tcccatcatc tcgggtgacg ccggttgcta cgtgctcgtc cccatagcgc
     2521 cgcatgcgct ggtcagcagg ccccttgtgc ttggcgagga ccaggtcgcc gagctggagc
     2581 tggtcgagcg ggaggcgctg ctctcgctgg acggggccga accgcgtgag atgccacctg
     2641 gcggaagggt ggaagtcagg ctctccagcg ctagcgttag aataggccgt accgaagact
     2701 ggagttggtg gcgagcagtt cggaggacgt tcctgtagtg cttacggagc tctccgtgga
     2761 gaacgtggcc ctgatcgaag aggccacgct cgaactctcc cccggcctca acgtgatcac
     2821 cggtgagacg ggggccggta agacgctcct tgctaccgcg ctgcagatgc tcctcggtgg
     2881 ccgcgcccgc tccgagacgg tccgctcggg cgcacgcagg gctaccgtcg aaggcacttt
     2941 cgtcctccct gaagagacgg gatctcggtt cctcaacgaa gccctgggcg acctcgccga
     3001 ggaggtggaa gacgatgatg gcctcgtcct gcggcggacg ctgaccgggg agggccgctc
     3061 cagatgctac gtgggcggcg tctcggtctc agtaaaggcg ctcgcttctc tcggggagag
     3121 gctggtctca taccacagcc agcgggagca aacacggctc accgagccgg ccgatcaact
     3181 cgccatcctc gacgacttcc ttgaccctgc ggctgccgag gcgaggcatg agcatgaact
     3241 gatctggcgg gccgtggaga acgaccgcgc cgcgcttcag gagataagga cgacctctga
     3301 ggcgcggctg cgggaggtgg agttcctgcg ctaccaggtc tctgagctcg agtcagcggg
     3361 ctacgaccag caggagctct tggacctctc ccgcgaacgc gaccgtctaa ggaacgtgac
     3421 tgaccttctt cagaccacat ccgccgcgag cgcgggcctt tcgtccgaag acggcacggg
     3481 agccgtagac gctgtcgcgc gcgccgcatc agagctggag acggcctcgg ggtatgacga
     3541 cagcctgagt gggctcctga agcggctgca cgggatctcc gcggagctgg acgacgtact
     3601 gtatgaactc aggtcctact tggaagaact cgaggctgac ccgaaccggt tggaagctgt
     3661 cgaggaccgc ctcgctgcca tgcgtgccct ggagcgcaag tatggagacg tcgagggata
     3721 cctccaggaa tcgcgcgagc gcctttcgcg gctcgaaaac ctggatgagg agaccgccgg
     3781 gctcgaagaa aggctcgcgg aaggcgagcg gcgcctcgag gtcatagcgg ccgagttgcg
     3841 ttcgggcagg gaggccgccg cacagaggtt gtcgggtaag gtgcaggaga acctcgcgga
     3901 tctcaacctc gggaagacga ccttccgcgc cgagcttgtc ccagcagagc caggcccgtc
     3961 gggaacggag agggtggagt tcatgatcca acccaaccct ggagagcccg agttgcccgt
     4021 gaggcgctac gcctcgggcg gggagctctc caggataatg ctggccataa ggctcgccca
     4081 ggagcgcgtg gagcctggtg cgacgtacgt cttcgacgag gtcgacgccg gtataggcgg
     4141 cgagacggca acggcggtag gagcgaagct gagggaactc ggcgaccgca accaggtcct
     4201 taccattacc cacctccctc agatagcctc cggggcgggc acgcacgtca tcgtcgaaaa
     4261 ggaagaggca ggaggacgga cgatcacccg catagagcgt atcgagggcg aggagcgccg
     4321 cagggagcta gccaggatgc tctcaggaag gatcgacgag gcctccctgg cacacgcctc
     4381 tgaactcctg gtcagcggga gataggggcc cctccggcct gcccatcgtc gccgtaaggt
     4441 gccatcactc gacgatggta aagtgaagac caggatttgg ggatcgccgg cgacatagcg
     4501 cttatcctgg tggccgccct cctgggaggc atcttggccc gatggctcgg gctgcccctg
     4561 atcctcggct acatcgcagc tggcgtcctc atcgggccga acacgtttgg cccgacggtc
     4621 ggtgacgtac acgagataga gctcctggca gagatcgggg tggcgctgct cctgtttacg
     4681 atcgggctgc acttctccct g
//
LOCUS       DASTKM010000223         8780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1478202, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000223 DASTKM010000000
VERSION     DASTKM010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478202"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VFQ10_09160"
     CDS             <1..1314
                     /locus_tag="VFQ10_09160"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9965549.1"
                     /translation="NPTPRYAPWEKPPYSEFENKVLVVGGGFGGYTAATDLCKLIGKR
                     DDVGVLVIARDNFFTFWPMVPGIVSSDIDARNVAQPLRRALILAGASFRRARVKGVDT
                     NRKVVEAHGGLEFPYDQLIISLGGQPNFFGIPGVEEHSMTMRGVEDAVRLRNRVIERF
                     EEVSLIRDEVPESKLTFVVIGGGATGVEVASEIHTLVHENLAPDYPNIDPYRVRIYLI
                     EALPNILPELDPALRRAARARLLNERIEVLTGAMAEEITGDCVKLKGGKQIDTENVVW
                     TAGNRPNQTVQDLGLPYDERTGIKVDLTLRVEGHSDVWSVGDCAAVQDVHQEGAIVPP
                     TAQAAIQQGHLVARNVLKTIDGEGDLGEFEYKPLGQLVELGSNFAVNEVMGVRFTGMI
                     AAVFWRMAYLVRLNSPQSKVRVAADWIVGIFLRPAVTQISSTSEE"
     gene            1416..1985
                     /gene="pgsA"
                     /locus_tag="VFQ10_09165"
     CDS             1416..1985
                     /gene="pgsA"
                     /locus_tag="VFQ10_09165"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET9965550.1"
                     /translation="MSLANQLTLIRLVAVVPVMVALYLPFEWARPVAVVLYVAAILTD
                     YFDGIIARRNNEVTGFGKLMDSIADKALIVSLFFALVGEGSMAAWMAAIMVIREFAVT
                     GLRMVALEAGEVIAANRWGKAKMNAQSLAVFILLLGKPGAAGWQGVVSQIGWWVMLVA
                     VVLTILSGWSYLKDTPRILSITSKRDQRL"
     gene            2008..2850
                     /locus_tag="VFQ10_09170"
     CDS             2008..2850
                     /locus_tag="VFQ10_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965551.1"
                     /translation="MEGSNDRGLRPDEVEMILRRASELNSRRWTRDSYDTPSVSPEVL
                     VQVAAAAGIPEQDVRRAMWDMASEKTAEPRSFARKFYGPARLRAVREIERPAESTREY
                     LEGLLRLQQGLKLRRRTEVGSLWDPGDELGVVRRTLDFSGDRTLLKASLVELRVEGLG
                     EERCESNLTADLSNQRSEYLSLAGILGATLAALFMLAGMQSPLFFLGVLPVLAAPALG
                     FRFAYSRACLELRRALDDLLDDAEESQPPEEPQDRGDRPRGQIQGLEPIPRFSPDRSK
                     RVEP"
     gene            2998..5154
                     /locus_tag="VFQ10_09175"
     CDS             2998..5154
                     /locus_tag="VFQ10_09175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HET9965552.1"
                     /translation="MPLKVLKEIFGFDSFRPGQEEVIRAVLEGRDTLAVMPTGGGKSL
                     CYQVPALMQDSLTVIVSPLISLMKDQVDSLLQSSVSDAATLHSGLSPEERWEVERRVR
                     TGEVKMLYVAPERLRSLEFVLSLRRAGVGLFVVDEAHCISEWGHNFRPDYLFLPRAVR
                     DLGNPPVLALTATATPRVRQDILVSLKMRSPEVVVTSFNRPNLTYRVIPAKEKKHKLP
                     RILDVIRASRPPGIVYATTRKECEELAAGLRRSGVDAAAYHAGMGSADRVNIQERFMT
                     DELAVVVATIAFGMGVDKPNVRFVIHSSVPGSLPAYIQESGRAGRDGEGSECVVLYRG
                     ADLGRRKRLVTLGTAGEREVSSFFRALSGVESGGRVNVPPASLSALGGVDPESAGIVM
                     GSLEESGLISRGYDLWAEIEVRAVEEEPKGLREEVAAVHAALPGSGSVGLPELARKAG
                     MRPAVVQGAIYRMMVDGVVEVIPRGSLVDVRLKTFSLDNLSRRNIAGRLKNRANTAYA
                     QIRDVEAFANLRTCRREHLLRHFGDMEEVAPCAGCDVCLGETLEHVRTAGPGTSVPEA
                     KPLVPAFAGSSRADEDLPGVDAALFERLRSWRGEQAKKQRVPAYVVLHNSHLEEISAR
                     KPGTIHELGAIKGVGLRRAARYGDEILALIRGEEAPDTAPPAENGFREHLEAADKLLR
                     TGRGADAVPALARALELGGEEARREVDELLGSMTRQ"
     gene            5177..5698
                     /locus_tag="VFQ10_09180"
     CDS             5177..5698
                     /locus_tag="VFQ10_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET9965553.1"
                     /translation="MKLEGKKIAVLVAEGFEDLEYWVTVMRLQEEGAEVVSIGPDTEP
                     VSGKNALVAQADTTADGVDASELDGVVVPGGWAPDKLRRYPEITDLVRTVHDRNKPVG
                     IICHGGLVAISASLVDGARATGSLGIKDDLENAGATWVDKAAFREGNLVWGRVVPDIP
                     DFCRELVAALAEE"
     gene            complement(5808..7214)
                     /locus_tag="VFQ10_09185"
     CDS             complement(5808..7214)
                     /locus_tag="VFQ10_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965554.1"
                     /translation="MAKQMQKHGNRIVQNYDMDDTSSHEDIEVHKGDICAEQIDDVNI
                     AARRETMFLLPLAALTSIVLTHLILDETYPKDYTYFTGDLAVLMGVLVATFFWWEFAH
                     YYRRYTTAASASRRNYNGLRERLDQLQNRVDHSGQGERATTGIPDVRQQAHAYAQEQC
                     TEIEKALKSRGTPWTTGLGYIELWHRVHRAEEALIKVEPYIEALRGAMRDESRLMNAT
                     MANRESLLKRLRCAVAMLDDSGTDRYLSYVEESEKCSLKQEERAKPKNRAKTLAILSE
                     VRYEINYFRDNVWEGIVHARNRLAETSVVLGIAAYALLALAIFANTPNKIIIWVVTYF
                     LVGAIIGLFARAQGEWNANTAVDDFGLSTARLIHTPWLSGLAAVGGVLFTSILDSQLL
                     NNGSDTSTLADVFKERPSLLLVAAVFGLTPDLIIRRLTQQVDKYEEDLQSTQSSQSEE
                     DIGTSETEKRRTTRRQAG"
     gene            7557..7988
                     /locus_tag="VFQ10_09190"
     CDS             7557..7988
                     /locus_tag="VFQ10_09190"
                     /inference="COORDINATES: protein motif:HMM:NF040238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HET9965555.1"
                     /translation="MAGEEVAPFLQEFAGYALTTDTSHELALWLYGPPGGGRSTFLAG
                     LGAMLGPRAGLLGLSEIQRNRFALADIPGKTLLTAIEQAAGYLRTSHVLNALISGEPI
                     QVEKKYRDPFVLVPRAKIAWVHLKAVVARPCRRLRRRCCWR"
     gene            8125..8568
                     /locus_tag="VFQ10_09195"
     CDS             8125..8568
                     /locus_tag="VFQ10_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965556.1"
                     /translation="MAEIERQSREPEGLDYVKVSLGLVFVFGAFVFGILAPPGTLVVS
                     FGLMIPAVALLASAFQEANKRRRALPLASDKERELLSAIRDNGGVITPMEAAMETSLT
                     VKEADRRLSELAGGGHLSVESAGGTLCYSLPGRQATELDEPSSAT"
BASE COUNT         1658 a         2641 c         2800 g         1681 t
ORIGIN      
        1 aaccccacgc cccgctacgc tccctgggag aagccaccct acagcgagtt cgagaacaag
       61 gtcttggtcg tgggcggagg attcggaggg tacacggcgg cgacggatct gtgcaagctg
      121 atcggcaagc gcgatgacgt cggggttctg gtcatagcgc gcgacaactt ctttaccttc
      181 tggccgatgg tgccagggat cgtcagcagc gacatcgacg cgcgcaacgt cgcccagccc
      241 ttgaggcgag ccctcatcct cgccggggca agcttcaggc gggccagggt caagggcgta
      301 gacacaaata ggaaggtcgt cgaggcccac ggaggtctcg agttccctta cgatcagctc
      361 ataatctcat tgggcgggca gccaaacttt ttcggcatcc ccggtgtcga ggagcacagc
      421 atgaccatgc ggggggtcga ggacgccgtg cgcctccgca accgcgtcat agagcgcttc
      481 gaggaggtct ccctcattcg cgacgaggtg ccagagtcca agctcacttt cgtggttatc
      541 ggtggtgggg ctaccggggt cgaggtggcc tccgagatcc acaccctcgt ccacgagaac
      601 ctcgcgcccg actaccccaa tatcgaccct taccgtgtca ggatctacct tatagaggcc
      661 ctgccaaaca tcctgcccga gctcgacccc gccctgcggc gcgccgcccg agccaggctc
      721 ctcaacgagc gcatcgaggt cctgacaggt gccatggccg aggagatcac gggcgactgc
      781 gtcaagctga agggcggcaa gcagatcgat acggagaacg tggtctggac cgcgggcaac
      841 cgtccgaacc agacggtaca ggacctcggc ttgccctacg acgagcggac aggaataaag
      901 gtagacctta cattgcgggt ggagggccac agcgacgtct ggtccgtcgg ggactgtgcg
      961 gccgtccagg acgtccacca ggaaggcgcg atcgttccgc cgaccgcgca ggccgcgatc
     1021 caacaggggc acctggtcgc ccgtaacgtg ctcaagacca tcgatggtga gggtgatctg
     1081 ggagagttcg agtacaagcc gctcggccag ctcgtcgagc tgggaagcaa cttcgccgtc
     1141 aacgaggtga tgggcgtgag gttcaccggg atgatagcgg ccgtattctg gcgaatggct
     1201 tatctggtgc gcctgaacag cccgcagagc aaggtgcggg tcgctgcgga ctggatcgtg
     1261 ggcatcttct tgaggcccgc cgtcacccag atcagcagca cctccgaaga atgatgatct
     1321 tgaggtgcgg ccacaaaggt gctacgttga ctgtggttgg aggaggcgaa gatgcggcgg
     1381 gacagtgtga cgcggtgtga gagagggggg ctcgcttgag cctcgccaac cagctcaccc
     1441 tgatccggct cgtcgccgtg gtgcccgtaa tggtcgcgct gtacctgccc ttcgagtggg
     1501 cgcgtcccgt cgccgtcgtt ctttacgtgg ctgccatcct gaccgactac ttcgacggga
     1561 tcatcgcccg ccgcaacaac gaagtcacgg gcttcggcaa gctgatggat tccatagccg
     1621 acaaggccct catcgtctcc ctattcttcg cgctcgtggg ggagggctcg atggccgcgt
     1681 ggatggccgc gatcatggtt atccgggagt tcgccgttac tgggctcagg atggtcgccc
     1741 tcgaggcggg ggaggtcatc gccgcgaacc gctggggcaa ggcgaagatg aacgcccaga
     1801 gcctcgccgt tttcatactc cttctgggca agccaggggc ggccggctgg cagggagtcg
     1861 tctcccagat agggtggtgg gtgatgctcg tcgccgtggt gcttaccata ctatcgggct
     1921 ggtcctattt gaaggacacc ccgcgtatcc tgtcgattac ctccaagcga gatcagaggc
     1981 tctaaaacct gtagaatcac gccccatatg gagggcagca atgacagggg cctgaggccg
     2041 gacgaagtcg aaatgatctt gcgccgcgcc tcggaactga actctcgcag gtggacgcgt
     2101 gactcctacg acaccccttc cgtctctccc gaggtcctcg tacaggtggc cgctgcggcc
     2161 ggtatccctg aacaagacgt gcgccgggcg atgtgggaca tggcctcgga gaagacggcc
     2221 gaaccgcgct cgttcgcccg caagttctac ggtcctgccc gtctgcgggc cgtgcgagag
     2281 atagagcgtc ccgccgagag tacccgcgaa tatctcgaag ggctgttgcg cctccagcag
     2341 gggctcaagc tcaggcgtag gacggaggtc gggtctttgt gggatcccgg cgacgagctc
     2401 ggtgtcgtgc gaaggacgct agacttttcg ggggacagga ccctgctcaa ggccagtttg
     2461 gtcgaactgc gggtcgaggg tttgggcgaa gagagatgcg agtccaacct gacggccgac
     2521 ctctccaacc agcgcagtga gtacctctcc ctcgccggca tcctgggggc cacgctggcc
     2581 gcattgttca tgctcgccgg gatgcaaagc ccgctcttct tcctcggcgt cctgcccgtg
     2641 ctcgcggcgc cggccctcgg gttcaggttc gcctacagca gggcttgctt ggagctgaga
     2701 cgcgccctcg acgacctgct cgacgacgcc gaagagagcc agcctccaga ggaacctcag
     2761 gaccgcggcg atcgtccccg cggccagatt caaggcctcg aacccattcc aagattctcc
     2821 cccgacagga gcaagcgggt agaaccctga gagcattgca gcaagtcagc tcgctgtttc
     2881 cgctcggagg cggatgcgcg gttcgcagca ggtggatacc actgtaaatc gctttgttct
     2941 agagggccgg gaatctcctc tttgtggaag tagagatcag gccttatatt taccatcgtg
     3001 cctctcaaag ttctaaagga gatcttcggc ttcgattcgt tcaggccggg ccaggaagag
     3061 gtcatacggg ccgttctgga aggcagggac acgcttgccg tgatgcccac tggcggcggc
     3121 aagagcctct gttaccaggt gcctgcgctg atgcaggata gcctgacggt gatagtctcg
     3181 cccttgatct cgctgatgaa agaccaggtc gactccctgc tacagagctc tgtctccgac
     3241 gcagccacgc tccactccgg gctctcgccc gaggagcgct gggaggtcga acggagggtt
     3301 cgcactgggg aggtgaagat gctctacgtg gcccccgagc ggctcaggtc tctggagttt
     3361 gtcctctcgc tccggcgcgc aggcgtcggc ctgttcgttg tagacgaggc ccactgtatc
     3421 tcggagtggg ggcacaattt ccgcccggac tacctgttct tgccgcgcgc cgtaagggac
     3481 ctcggtaacc ctcccgtcct cgcccttacg gcaacggcca ctccgcgcgt gcggcaggac
     3541 atcctggtgt ccctgaagat gcgctcgccc gaggtggtcg tgacgagttt caatcgcccg
     3601 aacctgacct acagggtcat ccccgcgaag gagaagaaac acaaacttcc acgcattctc
     3661 gacgtcatac gcgcctctcg gccgccgggg atcgtctacg ccacgacgcg caaggagtgc
     3721 gaggaactcg ccgccggcct gcgccgttcc ggggtcgacg ccgccgcgta ccacgccggg
     3781 atgggctcgg cagacagggt caacattcag gagcggttca tgaccgacga actcgccgtg
     3841 gtcgtcgcga ctatcgcttt cgggatggga gtggacaagc cgaacgtcag gttcgtcatc
     3901 cattcgagcg tccctgggag cctccctgcg tacatccagg agtctgggcg cgctggcagg
     3961 gatggggagg gatcagagtg cgtcgtgctc tatcgaggcg ccgacctcgg ccggcgcaag
     4021 cgtctggtta cgcttggcac ggcaggagag cgtgaggtct cctccttctt cagagcgcta
     4081 tccggggtgg agagtggtgg gagggtaaac gtaccgcccg cctcgctctc agcgctaggg
     4141 ggcgtcgacc ccgagtcggc cggaatcgtc atgggcagcc tcgaggagtc tggccttatc
     4201 tcccgcggat acgacctctg ggccgagatc gaggtgcgcg ccgtcgaaga ggagccgaaa
     4261 ggcctgcgtg aggaggtggc cgcggtgcac gctgcgcttc caggctctgg ttctgtgggg
     4321 cttcccgagc tggcgcgtaa ggcggggatg cgtcctgctg tagtccaggg cgccatctac
     4381 aggatgatgg tcgacggagt ggtggaggtc atcccccgcg gctctctggt ggacgtgagg
     4441 ctcaagacct tctcgctgga caacctcagc cgcaggaaca tcgcaggcag gctcaagaac
     4501 cgggcgaaca cggcctacgc ccagatccgg gacgtagagg cgttcgccaa cctgaggacc
     4561 tgccgccgcg agcacctcct caggcacttc ggagatatgg aagaggtcgc accctgcgcc
     4621 ggctgcgacg tctgcctcgg cgagacgcta gagcacgtga ggaccgccgg gccaggaacg
     4681 agcgtacccg aagcgaagcc cctggtgccg gcgttcgccg ggtcgtcaag ggcggatgag
     4741 gatcttccag gcgtggacgc agcgctcttc gagcggctga ggagctggcg gggtgagcag
     4801 gcgaaaaagc agcgggtgcc tgcctacgtc gtgctgcaca actcgcacct ggaggagatc
     4861 tccgcccgca agccaggcac catccacgaa ctgggtgcca taaagggagt aggcctgcgc
     4921 cgcgcggccc gctacggtga cgagatactg gcactgatac gaggggagga ggcgccggac
     4981 actgcgcccc ctgcggagaa cggcttcaga gagcacctgg aggcagcgga caaactgctg
     5041 cggacagggc gcggcgccga tgcggtgcct gcgcttgccc gtgctctgga gctcggcggt
     5101 gaggaggcgc ggcgcgaggt ggacgaactt cttggttcaa tgacacgcca gtgaagacta
     5161 taagagggga gtctctatga agttggaagg gaagaagatc gcagttctcg tggccgaggg
     5221 tttcgaggac ctcgagtact gggtcacggt gatgcgcctg caggaagaag gtgcagaggt
     5281 cgtctccatc ggacccgaca ccgaaccggt cagcggaaag aacgcgctgg tggcgcaggc
     5341 cgacacgaca gcggacggtg tcgacgcctc ggagctggac ggcgtcgtgg tgcctggggg
     5401 ctgggcgcct gacaagctga ggcgctatcc cgagatcacg gacctcgtca ggaccgtgca
     5461 cgacaggaac aagcccgtcg gcatcatctg ccacggcggg ctcgtcgcga tctctgccag
     5521 cttagtcgat ggcgcgaggg ctacgggctc gctggggatc aaagacgacc tggagaacgc
     5581 cggcgcgacc tgggttgaca aggcggcgtt ccgtgaaggc aacctggtct ggggccgcgt
     5641 ggtccccgac atccccgact tctgccgcga actagtggct gcgctggcgg aggagtagag
     5701 acaaggggtc ggtacacaag tcggcacacc aatacaggga cgctgtgggc tagggtagtc
     5761 ggctactctt atttgaccgc ctgttcgaag gaacttacct aacgcgatca ccctgcctgt
     5821 cgcctagttg tgcgtcgctt ttcggtttca cttgtaccaa tgtcctcctc gctctgacta
     5881 ctctgggtgc tttgcagatc ctcctcatat ttgtcgacct gttgcgtcaa acgccgaatg
     5941 atcaggtccg gagttagacc gaagacggcc gcgacgagaa gtaggctggg acgctccttg
     6001 aacacgtcgg cgagcgtcga tgtatcagat ccgttgttca aaagctggct gtcgagtatc
     6061 gaggtaaaca gcacgcctcc cacagcggcc aaacccgaaa gccaaggggt gtggatcaag
     6121 cgtgcagtgg atagcccgaa atcatctacc gccgtattcg cgttccactc gccttgcgcc
     6181 cttgcgaaga gtccgatgat cgcgccgacg agaaagtagg tgaccaccca gatgataatc
     6241 ttgttggggg tgttcgcgaa gatggcaagc gctaagagcg cgtacgcggc gattcctaac
     6301 actaccgagg tttccgctag gcggttgcgt gcgtggacta ttccttccca gacgttgtcc
     6361 cggaagtagt tgatctcata gcgcacttcg ctgagtatgg ctaacgtctt ggcacgattt
     6421 ttcggcttag ctcgttcctc ttgctttagc gagcatttct cggactcctc aacatagctt
     6481 aggtatctgt cggttcctga gtcgtccagc atggcgacgg cacacctcaa gcgtttgagc
     6541 aacgattccc tgttcgccat agtggcgttc atgagccgtg attcgtcgcg catcgctcct
     6601 cggagcgcct ctatgtaggg ttcgaccttg atcaacgcct cttccgcgcg atgaacacga
     6661 tgccaaagct cgatgtatcc gaggcccgta gtccacggcg taccccgact tttcagtgcc
     6721 ttttcgattt ctgtgcattg ttcttgggcg taagcatgag cttgttggcg cacgtcgggg
     6781 atgccggttg tggctctttc tccttgacca gagtgatcca cccggttctg caactggtca
     6841 agcctctcgc gcaggccatt gtagttgcgt cggctagcgc tcgcagcggt agtgtaacgt
     6901 cgatagtaat gcgcaaactc ccaccaaaaa aaggtcgcaa ctaagacgcc cattagaacc
     6961 gccaggtcac cagtaaaata ggtgtagtcc ttaggatacg tttcatcgag tattaggtgt
     7021 gttagaacga ttgaggtaag agcggcgagg ggcaggagaa acatcgtctc cctacgcgca
     7081 gcgatattga cgtcgtctat ttgctctgca cagatgtcgc ctttgtgcac ttcgatgtct
     7141 tcgtgcgaac tcgtgtcgtc catatcgtag ttctgaacaa ttctgttacc gtgcttctgc
     7201 atctgcttag ccattagagt actctcttgc ttgtgccctc atatcgatcg tacttattct
     7261 ttatgagaac acgcaccgat gaatcgacga tgaatcgaga gggaaacgtt cgcaagagac
     7321 aaatagcatt tggaacaact ctgtgtaaga tgacacggac gtcccgcgtt ttctacgcta
     7381 tatataagta ttgaatctct gtcaactaga tcttgcccat tgactaccaa catgcatatt
     7441 tgtcgatgct actctcccag cgcgtcctcc atcgcgatcg cagcagcttc tcccagcccc
     7501 ggtatcacgt ggcctcttca gcgaaggcac ccaccaagga gcgatttctc agggaggtgg
     7561 caggggagga ggtcgcccct ttcctgcagg agttcgccgg ctacgccctc accaccgata
     7621 cctcccacga gctggctctc tggttgtacg gacctccagg gggcggacgc agcaccttct
     7681 tagctgggct tggtgcgatg ctcggtcccc gggccggact actcggactc tccgagatcc
     7741 agcgcaaccg ctttgccctc gcagacatcc cgggcaagac tctgctgacg gccatcgagc
     7801 aggccgccgg atacctgagg accagccacg tcctgaacgc cctaatatcg ggcgagccca
     7861 tacaggtgga gaagaagtac cgcgacccgt tcgtactcgt cccgcgagcc aagattgcgt
     7921 gggtccacct gaaggcggta gtagctcgcc cctgccggcg tctccgtcgg cggtgctgct
     7981 ggcgttagcg tccatgccgc tcgtacctga ccttggtccc cgcgaacgac gccggtcgcg
     8041 cgagagcccc gccaccttcg aacggttgag ctagaatgtt cggtaacgta gaagagaacg
     8101 agatcatggc cgtggaggag agagttggct gagatcgagc gccagagccg cgagccggag
     8161 ggcctcgact acgtcaaggt gagcctgggc ctcgtcttcg tcttcggcgc gttcgttttc
     8221 ggcatccttg ccccgccggg cacgctggtg gtctccttcg gcctgatgat cccggctgtc
     8281 gcgctcctcg ccagcgcctt ccaagaagcc aacaaacgac gacgtgcgct cccgctagcc
     8341 tccgataagg agcgggagct actgtcggcc atccgggaca acggcggcgt catcacccct
     8401 atggaggcgg cgatggagac atcgctcacc gtgaaggagg ccgaccggag gctctcggag
     8461 ttggccggag gtgggcacct ctccgtagag agcgcgggcg ggacgctctg ctattcgctc
     8521 cccggcaggc aggcgaccga gctcgacgaa cccagttccg ctacctaaca cgttccgccc
     8581 ccgccggtgc cccctccacg tcggttgagg cagcctctag ggcccatcaa tcgcgcggaa
     8641 gattgctcag taccttatcg acgtagaatg ttgagttgcc tacgacttgg aggaggaacg
     8701 accatggcca acaatccgag agtggtcggc gaaggcgacc tggagtggag tgagcactcg
     8761 cacggggaga agttcgccta
//
LOCUS       DASTKM010000224         8594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_950970, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000224 DASTKM010000000
VERSION     DASTKM010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950970"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VFQ10_09200"
     CDS             <1..1040
                     /locus_tag="VFQ10_09200"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HET9965557.1"
                     /translation="TIAQFDLHHPSRHAAADPRNVHGHLLIPTRSVDERGRFDNGAKM
                     RVLSNRTQARNEVKALRGLWVETVNEELAREGIVREDGSPLRLDPRSYSERGIERIPE
                     VHLGVAATALERQGVPTGLGNQNRTIREDNAELARLDEELAQLERERHLLLKPSAPSV
                     TPGGATEDPKPGNSADHQLDVRVPIPTGLASDVMAYRELIGIEAAERELLDRISKAEQ
                     DSRVTVDEALREALEAFGIQMRIAFQDGSGAEKSWREYLTRHGYQEAVATLRDRPERI
                     GALRVRKTAFGLRSDDQAAREAAPRAAEAGARLAQELTRLERRRIEAEERRDRLVADR
                     KSPREWTLWVL"
     gene            complement(1086..1853)
                     /gene="istB"
                     /locus_tag="VFQ10_09205"
     CDS             complement(1086..1853)
                     /gene="istB"
                     /locus_tag="VFQ10_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HET9965558.1"
                     /translation="MPHADLDAMLTRLHLPTIRRLYGELAQRAEGEGMSYRTFLETLC
                     AEEIAHRAQTRITRSVRKARFPFLRTIEEFDFTFQNSIRLQMLGTLLGPEMVSEGRCA
                     LFSGPPGRGKTHLAVAISYRAIQNGFEARFTTADELIGELSQAALSGRLEAALEPYVH
                     PHVLAIDELGYQSYAADAANVLFRVVSIRHLKRRSTIVTTNKPLAALGQVLHDADLAE
                     AILDRLLERGTHYSLRGHSYRTRHHKEEIRPSAEDAA"
     gene            complement(1853..3250)
                     /gene="istA"
                     /locus_tag="VFQ10_09210"
     CDS             complement(1853..3250)
                     /gene="istA"
                     /locus_tag="VFQ10_09210"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET9965559.1"
                     /translation="MIDRHAVHALLQAGLPTKDIAAQIGVTQRTIQRIAKEPPVQESD
                     DAAARRLRGVGRPAVPEEVRQRVRSLIAADHDAPPLEVLRQLREEGLGLGESTFYRIF
                     RLEKEQLPAPLMVRFEGVAGEFAQFDFGQADVRLLDGRTHRIHFAAYRLKYSRWVWVV
                     LVPDERIESLVRALLLCFEHSGGVPLRVVFDNPKTVVLGRDEHGRPRWNATLAQVAID
                     YGFTIELCVPRSPEQKGSVENLVGWVKKSFLRARRFAELEHDLPRQLTEWLVEVNEER
                     PSRATKEVPAARLAAERERMKPLAVPPTEYALRLPVQVGPTALVDFQGIRYAMPAGAC
                     GLPATLHLYPDRVRITTAGGRFEATHPRFPRTGRTSYLDGQRSEQLAAVAGARKRLYF
                     MRERILELGPVGEGYLTELIHARPFTWKADVERLFMLLEELGESRFLKLLQRALFQRL
                     YGAEYVVRLAAEVAS"
     gene            3386..3562
                     /locus_tag="VFQ10_09215"
     CDS             3386..3562
                     /locus_tag="VFQ10_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965560.1"
                     /translation="MTLKRRDELRLRITDQWLALPVSVTRTLQATYPDIARLVEKSRR
                     GLQPDVGTRGDFNR"
     gene            3919..4407
                     /locus_tag="VFQ10_09220"
     CDS             3919..4407
                     /locus_tag="VFQ10_09220"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HET9965561.1"
                     /translation="MHRVPKYKVQLVREGSVPFSKRLCATPREAADLFRAYVGDSDRE
                     HLVAIFLDTQNRFLGLHTISVGTLDHSVVHPREVFKAAILSNASSLVLVHNHPSGEPA
                     PSDEDIRVTSMLLMVGELLDIPLMDHVVVGETTYSSLMEMGLLKEDMVETKPPRRRKA
                     KK"
     gene            4865..5794
                     /locus_tag="VFQ10_09225"
     CDS             4865..5794
                     /locus_tag="VFQ10_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HET9965562.1"
                     /translation="MQGNEHPQQEHAATPADPAPEMDVSDGDEPAASVPVWAHGVVEL
                     VPLDEIVLPAHQASVVSTPRSLTRLAASLRAPGRPLPPLPARRLEDGKYELLEGGRLL
                     EAARMLRWTHARLLVVEVDTARAAVWTIVSRCHTPRYRRWDRIRDVRYLIAVWGESVT
                     GRMFSQWTGWHQSDVSEFRRAAEVVTPEVFALAGVNEDLDIDLLNRLAREQIRYVFHG
                     GTEADVALRLREVMNGGMSADGVKDDGERERIRGAVNSELSPDGRWSAEVNLAEISVA
                     HRAYLRRLLLKQFNEACRRMPGGNGTASQPPQQ"
     gene            5917..6339
                     /locus_tag="VFQ10_09230"
     CDS             5917..6339
                     /locus_tag="VFQ10_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965563.1"
                     /translation="MNTKVPSKIRQLIERWHARVRSAEDELQDHRMGGVIRRMVTELE
                     EAIQAELDEFLDADEAAAFSGLGKDQLRRTIPNRGSKYRPRYRKGDLPTKASAPDESR
                     LPRILQLATEVPDSAEESRREQIRQQARETARQVRRAG"
     gene            6405..7862
                     /locus_tag="VFQ10_09235"
     CDS             6405..7862
                     /locus_tag="VFQ10_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965564.1"
                     /translation="MQQDPTSEFHHSRLPTAFEFPHVVERMGERLTLRRETGRARIMF
                     DYNPPDGAREKRVIPREAGDAGLPLPDSDFVPEDAVVWAEAFLEEKALAKSRFATELA
                     SSGVLATGTTIGFVFDQYRQSTEFANLRPKYQRDMTRVMDVAEAVLGRKFEIDAWDQE
                     ACDRLVEARGRGLRFDLPDGRQRQFHRAGKTTCRTMMVMLRRIFRWAMGRKHPRKPGT
                     WLLPHDPFARVRMPKKGVARVSILTHDRYLVMLDFADEIDATGRFRLILADARWTGHR
                     LGAICQMLRSDLLLTAEEIEEALHRSQCKYVAPDQIRQVAEFYSRYGAIYRRWEVQKQ
                     GASGEEEVVQYDRVVPLGPAHRAEINRYLEHHWEALGLLHDAPLYPSEHNDTRCLYID
                     LPEKWWLACEALARERGHRLAKLERTRFHGFRRLRRTELKQTKIHDKDVAFVCGWTIH
                     NFPEAKASAAMNGLYLGFIPEDLLEAACVGEGNVG"
BASE COUNT         1583 a         2749 c         2906 g         1356 t
ORIGIN      
        1 gcaccatagc tcagttcgac ctgcatcatc cgagtcgaca cgcggcggca gacccgcgca
       61 acgtccacgg tcacctgctg atccctaccc gaagtgtcga tgagcgggga aggttcgaca
      121 acggcgccaa gatgcgggta ctctcgaatc ggacccaggc ccggaacgag gtgaaggccc
      181 tccggggcct ctgggtggag acggtcaacg aggaactggc gcgggagggc atcgtgcgcg
      241 aggacggctc tccgctgcgc ctggatcccc ggagctatag cgagcgcggg atcgagcgga
      301 ttccggaggt gcacctcggg gtggccgcga ccgcgctcga gcggcagggc gttcccactg
      361 ggctcggcaa ccagaaccgc accatccgtg aggacaatgc cgagttggcg cgcctggacg
      421 aggagcttgc ccagctggag cgggagcggc atctgctcct caaaccctct gcgccgagcg
      481 tgacacccgg tggggcgacg gaggatccga agccggggaa ttcggcggac caccagctgg
      541 acgtgcgtgt cccgatacct accggcctcg cctcggacgt catggcatat cgggagctga
      601 tcgggattga ggcagccgag cgcgaactcc tcgatcggat cagcaaagca gagcaggaca
      661 gccgggtgac cgtcgacgag gcactccgcg aagcgctgga ggcattcggc atccagatgc
      721 ggatcgcgtt ccaggacggc agcggggccg aaaagagttg gcgtgagtac ctgaccaggc
      781 acgggtacca ggaggcagtc gcaaccctcc gggataggcc cgagcggatc ggcgcactgc
      841 gggtgaggaa gaccgcattc ggattgcggt cggacgacca ggctgctcgg gaggcagcgc
      901 cgcgtgcggc tgaggcggga gcccggctcg cgcaggagct gacccggctg gagcgcaggc
      961 ggatcgaggc ggaggaacga agggaccggc tcgtggcgga tcggaaatcg ccgcgggaat
     1021 ggactttgtg ggttctctga attctgtcgc ggtttctctc taaactttgt cgccctcggg
     1081 cggagctatg cggcgtcttc cgcggacggg cggatctcct ccttgtggtg tcgggttcgg
     1141 taggagtggc cgcggagcga gtagtgcgtg ccccgctcca gcagccggtc gaggatcgcc
     1201 tcggcgagat cggcatcatg caggacctgc ccgagcgccg ccagaggctt gttcgtggtg
     1261 acgatcgtgg agcggcgctt gaggtggcgg atgctgacga cgcggaagag cacgttggcc
     1321 gcatcggcgg cgtagctctg gtagccgagt tcgtcaatgg ccagcacgtg cgggtgcacg
     1381 tagggctcca gggccgcttc gagccgtccc gagagcgccg cctgtgagag ctccccgatc
     1441 agctcgtcgg ccgtggtgaa gcgtgcctcg aagccgttct ggatggcccg gtaggagatc
     1501 gccaccgcca gatgcgtctt gccccggccc ggcggtccgg agaagagggc gcagcggccc
     1561 tcgctgacca tctccggccc caggagggtg cccagcatct ggagccggat ggagttctgg
     1621 aaggtgaagt cgaattcctc gatggtgcgt aggaagggaa acctcgcctt gcgcacggag
     1681 cgcgtgatcc gggtctgggc ccggtgggcg atctcctcgg cgcagagcgt ctccaggaag
     1741 gtgcggtagc tcatcccctc gccctcggcg cgctgggcga gctcgccgta gaggcggcgg
     1801 atggtgggca ggtgcagccg cgtgagcatg gcgtccagat cggcgtgcgg catcacgagg
     1861 ccacctccgc ggcgaggcgc accacgtact ccgcgccgta gaggcgctgg aagagggcgc
     1921 gctggagcag cttgaggaag cggctctccc caagctcctc cagcagcatg aagagccgct
     1981 cgacgtcggc cttccaggtg aaggggcggg cgtggatcag ctcggtcagg tagccctcac
     2041 ccacaggccc gagctccagg atccgctcgc gcatgaagta gagccgcttg cgggctccgg
     2101 cgaccgcggc aagctgctcg gagcgctggc cgtccaggta gctcgtccgc ccggtgcggg
     2161 gaaagcgcgg gtgcgtggcc tcgaagcgcc ctccggcggt ggtgatgcgc acccggtcgg
     2221 ggtagaggtg gagcgtggcc ggaaggccgc aggccccggc cggcatggcg tagcggattc
     2281 cctggaagtc gaccagcgcg gtgggcccga cctgcacggg caagcgcagg gcgtattcgg
     2341 tcggcggcac cgccaggggc ttcatccgct ctcgctcggc ggccaggcgc gcagcgggga
     2401 cctccttcgt tgcccgcgag gggcgctcct cgttgacctc gacgagccac tcagtgagct
     2461 ggcgcggcag gtcgtgctcc aactccgcaa agcggcgggc gcgcaagaag ctcttcttca
     2521 cccagccgac caggttctcc accgagccct tctgctcggg gctgcggggg acgcagagct
     2581 cgatggtgaa gccgtagtcg atggcgacct gggccagtgt cgcattccag cggggccggc
     2641 cgtgctcgtc gcggccgagg acgacggtct tcgggttgtc gaagaccacg cgaagcggga
     2701 cgcctcccga gtgctcgaag cacagcagca gggcgcgcac gagcgattcg atccgctcgt
     2761 cgggaacgag cacgacccag acccagcgcg agtacttgag ccggtacgcg gcgaagtgga
     2821 tccggtgcgt acggccgtcc aggagccgca cgtcggcctg gccgaaatcg aactgggcga
     2881 actcgccggc cacaccctcg aagcgcacca tgagcggcgc gggaagctgc tccttctcca
     2941 ggcggaagat ccggtagaac gtgctctcgc ccagcccgag gccttcctcg cgcaattggc
     3001 gcaggacctc cagaggcggc gcgtcatgat cggccgcgat cagggagcgc acgcgctgcc
     3061 gcacctcttc ggggaccgct gggcggccga cgccgcgtag gcgacgggcc gcagcatcat
     3121 cactctcctg gaccggcggc tccttggcga tgcgctggat ggtgcgctgc gtcaccccga
     3181 tctgtgcagc gatgtccttc gtgggcagcc ccgcctggag cagggcgtgt acggcatgtc
     3241 tgtcgatcat ccgaagcatc ctcctccgcc tccggccgtg ggatcagcag cccttccgga
     3301 acggcggcaa ggtaggtctc aaagaccccg aatggcgaca gaacttagag aggatcggcg
     3361 acagagttta gggaacccac agactttgac gctgaagcgc agggacgagt tgcgactgag
     3421 gatcaccgac cagtggctgg cgctgcctgt gtcggtgacg aggacactgc aggctacata
     3481 tcccgacatc gcgaggctgg tagagaagtc gaggcgaggc ctgcaaccgg acgtgggtac
     3541 ccgcggcgac tttaaccggt gagcagcctg ctcgcggccg gcagcatagc gcaacgaggc
     3601 acctggagtt tcactgcgga tcaccgcgta ggttgcgcgt gaaggccctc acgggacacc
     3661 gacagtttcg cgcggcgaaa ccatgggcgc tgcgtagacc ttccccaccc cgaagctccg
     3721 tcccgaacag gaagaagaca tagaaacaag acggtgcggc gtcggttcgc tcccggttcg
     3781 gtaggctgcc gggccgcacc tcccccatgc ccgcctgacc ggcgaacccg gtcggcgggc
     3841 tttgatgcat ccccccatat ctcctaccac cggacctgtc gatcaaggca cgtccatcaa
     3901 gatcagaaag agccatccgt gcatcgcgtc cccaaataca aagtccaact ggttcgggaa
     3961 gggtccgtac cattcagcaa acgcctctgc gccactccgc gggaggccgc cgatctcttc
     4021 cgcgcctacg tcggcgactc cgaccgggag cacctggtcg ccatcttcct ggacacgcag
     4081 aaccggttcc tcggcctgca taccatctcg gtcggtaccc tcgaccacag tgtggtacat
     4141 ccgcgcgaag tgttcaaggc ggcgatcctc agcaacgcgt cgagtctggt cctcgtacac
     4201 aaccatccgt cgggcgagcc cgcgccgagc gacgaggaca tccgggtgac ctccatgctc
     4261 ctgatggtag gtgaactgct cgacatcccc ctgatggacc acgtcgtcgt cggtgagacc
     4321 acctattcct ccttgatgga gatggggttg ctgaaggaag acatggtgga gacaaagccg
     4381 cctcggcgcc ggaaagcgaa gaagtaggta agtatcgtga caattgagag agaccggtgg
     4441 acgtctcggg gaagatcctg aactcaggga ggatcgctgc ggctacggag gcccgcctaa
     4501 acatctgagt gcgtcacaac tgattgggtg acaaacaatt cgctgcgtag aaaagaaagg
     4561 gagtcggaaa atacgctttg gaaagggaca aatgcatgat ctggctatca tgtcgagtcc
     4621 ggccagcctg ctgtgcctgg ggatgcgggg tatgccgttg caggcccgca tccgagcgtg
     4681 gccctgccag taccacgtga ccctgacctt ccgcgaggaa cccatgagtt cgaccgctcc
     4741 agatccgcag tcacccgatg cccatggggc aagggagctc ccacaggaca ccgacgcttg
     4801 ggagctcccc ctcggtctcc cccctcgtct gcccggaatc agtgatccga aggcgcctcc
     4861 agcgatgcag ggaaacgagc acccgcagca ggaacacgcc gccactcctg ctgatcctgc
     4921 accagagatg gacgtcagcg acggcgatga gcctgccgcg agtgtgccgg tctgggcgca
     4981 cggcgtggtc gagttggtgc ctctggatga gatcgtcctc ccggcccacc aagcgagtgt
     5041 ggtctccacg ccgcggtccc tgacccgcct cgctgccagt ctgcgtgcgc ccgggcggcc
     5101 gctcccccca ctaccggccc gacgcctgga ggacgggaaa tacgagttgc tcgagggagg
     5161 gcgcctgttg gaggctgcgc gcatgctccg atggacgcac gcacgcctcc tcgtcgtcga
     5221 ggtcgacacc gcgcgggccg cagtctggac catcgtatcc cggtgccaca cccctcgcta
     5281 ccggcgctgg gacaggatcc gtgacgtccg atacctcatc gccgtctggg gcgagtccgt
     5341 cacggggcgg atgttctcgc agtggacagg ctggcaccag tcggacgttt ccgagttccg
     5401 gcgagccgcc gaggtcgtga cgcccgaggt gttcgctctc gcgggagtca acgaagactt
     5461 ggatatcgac ctcctgaacc ggctcgcccg ggagcagatc cggtatgtct tccacggcgg
     5521 taccgaggct gatgtggcgc tccgactgcg ggaggtcatg aatggcggta tgtccgcgga
     5581 cggagtgaag gacgacgggg agcgtgagcg gatcaggggt gccgtgaaca gcgagctctc
     5641 cccggacggg cgatggagcg cggaggtgaa cctcgccgag atctccgtcg cacaccgggc
     5701 atacctcagg aggctgctcc tgaagcagtt caacgaggca tgccgccgaa tgccaggcgg
     5761 caacggcaca gcttctcaac caccgcagca gtagcaaccc accaggcaca tccgcgcctc
     5821 ccggtcatca caccgtgcag ctccagggca gtacggtgaa gacaccctcg cgcctgcggc
     5881 caccccaccc tcagatcctg tccaacacga actccgatga acacgaaggt cccgtccaag
     5941 atccgacaac tgatcgagcg ctggcacgct cgcgtccgaa gcgctgagga cgagctccag
     6001 gaccaccgca tgggcggtgt catccggcgc atggttaccg aactggagga agcgatccag
     6061 gccgaactgg acgagttcct cgatgccgat gaggctgccg cgttcagcgg actggggaag
     6121 gaccagctga ggagaaccat cccgaaccgg ggcagcaagt accgccccag gtaccgcaag
     6181 ggcgacctgc caacgaaagc gagcgccccg gacgaatcgc gcctcccccg catcctccaa
     6241 ctcgccacgg aggtgcccga cagcgcggag gagtcccggc gggagcagat ccgtcagcaa
     6301 gcccgggaga ctgcccgaca ggtacgccgc gctggataaa tgtctcctgg gggacctccg
     6361 ccctcccttc atcccaccat cactgaccac ccttcgagat cgagatgcag caggatccca
     6421 cctccgagtt ccaccactct aggctgccta cggccttcga gtttccccac gtcgtcgaaa
     6481 gaatgggcga gcgcctcacc ctccggcgcg agactggtcg cgcccgcatc atgttcgatt
     6541 acaatccacc ggacggagcc cgcgagaagc gcgttatccc acgtgaggcg ggtgacgccg
     6601 gtctcccctt gccggacagc gacttcgtgc ccgaggatgc ggtcgtctgg gccgaagcgt
     6661 ttctggagga gaaggcgcta gcgaagagcc ggttcgccac ggagctggcc agcagcggag
     6721 tcctcgcgac cggaaccacc atcgggttcg tcttcgacca gtaccggcag tccacagagt
     6781 tcgccaacct gcggcccaag taccagcggg atatgaccag ggtgatggac gtcgcggagg
     6841 ccgtgctcgg tcgcaagttc gagattgacg cctgggacca ggaggcgtgt gaccgactgg
     6901 tcgaggcgcg ggggcgggga ctgcggttcg acctccctga tggtcggcag cggcagttcc
     6961 accgcgcagg caagacgacc tgccggacga tgatggtgat gctccgccgg atcttccgct
     7021 gggcgatggg gcggaagcac ccccggaagc ccgggacgtg gctgctgccg cacgacccgt
     7081 tcgcgcgcgt gagaatgccg aagaagggcg tggcgcgcgt gtccatcctc acacacgacc
     7141 gctacttggt catgctcgac ttcgctgacg agatcgatgc gaccggccgc ttccgcctga
     7201 ttctcgccga cgcacggtgg acgggccacc ggctcggcgc aatctgccag atgctgcgct
     7261 ccgacctgct gctcacggcg gaggagatcg aggaggcgtt acaccgttcg cagtgtaagt
     7321 acgtggcgcc ggaccagatt cgccaggtgg cagagttcta cagccggtat ggcgcgatct
     7381 accgcaggtg ggaggtccag aagcagggcg cgtctggcga ggaggaggtg gtccagtacg
     7441 accgggtcgt gccgctcggg cctgcgcacc gcgcggagat caaccggtac ctggagcatc
     7501 actgggaggc actcggcctc ctccacgacg ccccgctcta cccgagcgag cacaacgaca
     7561 cccggtgcct ctacatcgac ctgccggaga agtggtggct tgcgtgtgaa gccctggcgc
     7621 gggagcgcgg ccatagactc gccaagctgg agcggacccg gttccacggc ttccgccggc
     7681 tgcgccgcac ggaattgaag cagaccaaga tccacgacaa ggatgtagcg ttcgtctgcg
     7741 gatggaccat ccacaacttc ccggaggcca aagcgtccgc cgcgatgaac gggctgtatc
     7801 tcgggttcat cccggaggac ctcctcgagg cagcctgtgt gggggaggga aacgtcggat
     7861 agggtacacc acagtagcag gcgggagacg gccattccgg tatgattggc cacaaccggt
     7921 gttggctcgc tggcggttgc cacgtcgtgt tgccgatacc agtacccggt aattaacttg
     7981 gccaccggct gcaaggttga ggcgtgagca gctgggtacc gtggaggaaa aggttgacgg
     8041 gatgtcgaac agcgagcaat ctacgagcca aagaggcggc actctctctc gacgaaccgg
     8101 atgagttccg aagaaccaag cgcaggcgga atggttctgc atccggggaa caatgccaga
     8161 gtgctccttg cccatcagac caggactaac ctataggcgg tccccaatcc tcccactttg
     8221 aatcggcgat ttctgccgcg atctgagatt cgggagggaa ccggaacaac ggctcagcct
     8281 tcgccgggat ctgggtagct atcgccgatc gacccgaaga gcatcgggcg tagtgaagca
     8341 cattcgtatg ttcttgttcg aagcatacaa tcatcgagct aaccggggcg cagaggatgt
     8401 tggagcgtgc gcgtggttcg atgtcgcgaa aacatgtccg attctttcct ccccagagga
     8461 cgccgtgttc ctgctccggc accttgacga ggatcattag ccctcctgcg gatacactcc
     8521 aatgcgacca ccggccctcg tgttcttcgc aacagcgtgg ggagttcgcc acggaggcat
     8581 taacgctttc aacc
//
LOCUS       DASTKM010000225        11097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1478720, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000225 DASTKM010000000
VERSION     DASTKM010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478720"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFQ10_09240"
     CDS             <1..598
                     /locus_tag="VFQ10_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9965565.1"
                     /translation="EMVAIMGPSGSGKTTLLNVLSGLDDLSDGEVYVDGESIKGMSDR
                     RRTRFRAEKMGFIFQTYNLIPVLSAIENIELPLLVAGRKPKKARQWAQDALEMVGLAG
                     QAKKRPAEMSGGQQQRITVARSLVNNPAIVWADEPTGALDSETSKGIMDLLVRMNEQD
                     QQTFVLVTHDAAVAARAHRTIRMRDGRIASDESNGKGE"
     gene            599..3658
                     /locus_tag="VFQ10_09245"
     CDS             599..3658
                     /locus_tag="VFQ10_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9965566.1"
                     /translation="MEELFGVPAQQLMWILLAIFGAGALILGLAALRNRVSFRMAARN
                     LPRRRTQTILVVLGLMLATMLFSASFTTGDTLTNSLRVQSLENLGQVDVQVQADGSGA
                     SGQQQFGDTSSERASYFDAEVAGEVRDRLSDENHVSGVAPAAIETVPVTARSSDLSEP
                     STDVLGVDESSMKGFDRLTTSSGKKLDPTDLGKNEIYLSADAAKGLDVGEGDVIEATF
                     AQRSGATAAPNAPPAAAAVPGALPVPRPSELTVAGVYESGANPASSTSMVMPLQRLQE
                     RIGEEGEINAVLITHKGPAIEGAAGSGATRDALRVLLEENDLEAEPVKKEAIDQADEG
                     GETFTSIFLLFGQFSVGAGVLLIFLIFVMLAAERKHELGIARAVGMRRGHLMRMFAFE
                     GALYALLASALGSVAGVGVGWLMVRVIGKAFAGSGFEISFATNPENVVIAFCLGMVLT
                     FAVVLISSWRVSRLNVVRAIRDIPEPDRKGRSVKGILFALTTPLAGAGLVWQGLQAEQ
                     MGLYMLGLSLIIVGTALAARVLRVPERISFTVAGTLLLGLWLLPFSFAPSGMTEGIDL
                     FFISGVMIVLAGVWIIIYNSDLLLGAVVALFGWLRGMPPVLRAAVSYPMHSRFRTGMT
                     LAMFSLVVFTIVTMSFITAAFGSIFEDTDRLSGGFDVRADAGYAAPIPDMNEALKDKK
                     SVHAADFTAVGTLTGLSVDAKQKDTSRKPQSLFLQGVDGGYTKNVGYGFKSTVAEYDT
                     ANEVWTALRTEKDTAVISSDLAPSRSNFVGDEEPPITLSGFYGEDPKLPNDLYIQVED
                     PQSGRTRNLHVIGVLEDTAFFAPGVTSSRATVDDLAGSPLPAQSYQFRLADGVNSEAV
                     AKDLEKAFAQNGLQAVAIKKEIEDGSASQGLFNNLLMGFMGLGLLVGIAALGVIAARS
                     VVERRQQIGMLRALGFQRGQVRLAFLIESSFVALLGIGLGVALGAALSVGIVDSFEEQ
                     ISGIRYTVPWSTLGVIVGLAYFASLLTTFLPASQASRVYPAEALRYE"
     gene            3795..5279
                     /locus_tag="VFQ10_09250"
     CDS             3795..5279
                     /locus_tag="VFQ10_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9965567.1"
                     /translation="MMGEIFALRRLRWKLTLSYTLVAAVTLLALELILAGALVAFLNS
                     PLLPRLVVQNFRDEVAPRLERPLNQNPPDTEAIRERLVGFADSSGVRGADRPPPPPVS
                     PSNYAADQGALLVVDDRRRLLVSVPEMQGFPEGRRFDAGSISGLKPLVNAAFDGEENS
                     GQLSSNVEGQVVTVTPIEGKKGRVAGVLVGTLRLPNLTAPLLITVGIGAIFLTIPAAF
                     LGALFGFFTAWSLTRRIGRLVRAARSWSRGDFSVTTKDRSRDELGQLSRELNSMAAEL
                     ENLLQARGELATLEARNRFARDLHDSVKQQVFATSLQIAAARALIEKDVGSAESHLSQ
                     ADELVRQAQKELNVLIQEMRPAALEGKGLAAALREYVVRWSEGAEIRADFRVRGERRV
                     PLEVEQALFRVAQEALANVARHSGAGHAEVDLSYATDAITLRVADDGRGFDPSRNPGG
                     GFGLQSMRERLVRLGGHVNVESAPGKGTLVECFCPVQEKKGEDL"
     gene            5276..5929
                     /locus_tag="VFQ10_09255"
     CDS             5276..5929
                     /locus_tag="VFQ10_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9965568.1"
                     /translation="MSEPITVLLVDDHALVRQGVRAFLQTQGDISVVAEAESGEEAVR
                     LAAEHAPDVALMDLIMPGMDGVEATRRLVASSPRTNVVMLTSYHDDEHIFPAIRAGAL
                     SYVLKEIGPHELAEAVRKAAAGEAVLHPRVAARVVRELHGARRAEPNVFHDLSDRELE
                     VLKLIAEGLHNSEIAGRLYLSEKTVKSHVSNILSKLHLADRTQAAVYAWRQGVVRRD"
     gene            complement(6132..6719)
                     /locus_tag="VFQ10_09260"
     CDS             complement(6132..6719)
                     /locus_tag="VFQ10_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HET9965569.1"
                     /translation="MAKRSGRSSGSDGSRRTYLIVGAVIVVFVAGFIALAVLDARQQS
                     GGAASEDVKTYDVGPAGKHTGGEVDYEQSPPVGGAHNPVWQNCGYYDKPIRDESAVHS
                     LEHGAVWITYSPDLPQDQVDELRGIAESQSFVLVSPYPDLPSNTPVVASAWGKQVGLS
                     GADDPDLESFIQAYQQGPQTPEPGAVCTGGTSATL"
     gene            complement(6783..7163)
                     /locus_tag="VFQ10_09265"
     CDS             complement(6783..7163)
                     /locus_tag="VFQ10_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HET9965570.1"
                     /translation="MSPIGRLKRWALRLKVEVYALYLAYKDPRVPWYARAFAAVVVGY
                     AFSPIDLIPDPIPVLGYLDDLVIIPLGVALAIRMIPPQILAECREEARDAKDRPVSKM
                     TAMVVVAVWISLAALTVWLVARAF"
     gene            complement(7195..7884)
                     /locus_tag="VFQ10_09270"
     CDS             complement(7195..7884)
                     /locus_tag="VFQ10_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET9965571.1"
                     /translation="MSGQPLQMSRKLPGIGERERLVRRAKLLAWLGVGWHGVEAAIAV
                     GAGVVAGSIALIGFGADSLIESLAGVILLWRFAAARASSESAERSAQKLIGVSFYLIA
                     AYVGFEAVRSLLSGARPEASWMGIGLAAVTLVTMPPLAIAKARVGEKLGSSATRSEGQ
                     QNMLCAYLSAALLVGLGANALFGLWWADPVTALLIAGVAVKEGRESWRGESCCTAPVV
                     TGENCEDDCCR"
     gene            complement(7881..8234)
                     /locus_tag="VFQ10_09275"
     CDS             complement(7881..8234)
                     /locus_tag="VFQ10_09275"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9965572.1"
                     /translation="MNDERCDLLCLDVPRAEAIRVGLPGIEAAGEAAERARMFSDPTR
                     LVLAAALGEGEELCVCDLAWISSRAQNLVSHHLRLLRSHRLVTSRRSGKLVMYSLTEE
                     GRSLLHAVLGEKVIS"
     gene            complement(8333..9247)
                     /locus_tag="VFQ10_09280"
     CDS             complement(8333..9247)
                     /locus_tag="VFQ10_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9965573.1"
                     /translation="MGHHGHDHTANARDGNRRALAVVLVLTASFTVVEVIGGLLTGSL
                     ALLADAGHMLSDNVSLGVALFAAWLAGRPATPEKSFGYRRAEILAALANGITLVAISI
                     WVFVEAYARLREPAEVLGTPMLAVATLGLLVNAAGATILYRSGRGSLNVEGALRHVLA
                     DALGSVGAMVAAAIIILTGWRYADPLVSVAIGFLILGSSWTLLRDSTNILLEATPRGL
                     NAGEVGQKMAAAEGIIEVHDLHIWTITSGFPALSAHVLVGRHEDCHARRRELEELLTN
                     EYGISHTTLQVDHVGDHHTNLRFPSRLD"
     gene            complement(9304..10395)
                     /locus_tag="VFQ10_09285"
     CDS             complement(9304..10395)
                     /locus_tag="VFQ10_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9965574.1"
                     /translation="MKSRSTAISASLTFILTTILLTGCSETEPGPQAENTANVSPQAS
                     IPADEPVAKVASKVGPSVVQVNVRAVRETPLGIQRGEGLGSGVIYRSDGYIVTNNHVI
                     EGATGVNVAFADGTIEEASIVGTDPNTEIAVIRVNRNDLPAATFDDGDAPIVGQLAVA
                     IGSPSGFESTVTSGVVSGVGREFPPELTSGGQAEQNALTDLIQTDAAISLGNSGGALA
                     DRDGEIIGINVAYLPPAETGAVDLGFAVPSDTAVSVADQLIEEGEVTTPYLGVLTTDL
                     SPEDAGRFDLPIDSGALVVQVVPGSPAREAGVRKGDIITALDNARIVGYGDLLGALRD
                     HEPGDRVILTIFRNTQEKKLAVTLGETSQ"
     gene            10476..10835
                     /locus_tag="VFQ10_09290"
     CDS             10476..10835
                     /locus_tag="VFQ10_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965575.1"
                     /translation="MYPLRVDASTNRREVPADLARAGEARHFIAWRGAVAGLGGGRLN
                     ELAVAAEAVITDILLSAREGVLEIQSVHDEEVVRISIEHPEIEQRRMKDLERVLERYL
                     DDYEIIPTRAMLEKRLR"
     gene            complement(10849..>11097)
                     /locus_tag="VFQ10_09295"
     CDS             complement(10849..>11097)
                     /locus_tag="VFQ10_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965576.1"
                     /translation="LNRIQSLSNREREETLREAETMVGMADAAIKELESVLGNRRVNL
                     PEEHAVRFAQDVFLYLRDQEQKVKEALREPASRDAAEG"
BASE COUNT         1978 a         3576 c         3544 g         1999 t
ORIGIN      
        1 ggagatggtc gccatcatgg gcccttccgg atccgggaag accacgctgt tgaacgtcct
       61 ctccggcctc gacgatctct ccgacggcga ggtctacgtg gacggcgagt ccataaaggg
      121 catgtccgac aggaggcgga cacgctttag agccgagaag atgggcttca tcttccagac
      181 ctacaacctg atccctgtcc tctcggcgat cgagaacatc gagcttccgc tcctggtcgc
      241 cggcaggaag ccgaaaaagg ctcgccagtg ggcgcaggac gccctcgaga tggtggggct
      301 cgcgggtcag gccaagaaac gtccggccga gatgagcggt ggccagcaac agcgcataac
      361 cgtcgcccgc tcgctcgtga acaacccggc catagtctgg gccgacgaac caaccggcgc
      421 cctggactcg gagacctcga aagggatcat ggacctcctg gtcaggatga acgagcagga
      481 tcagcagacc ttcgtcctgg tcacccacga cgccgcggtc gcggccaggg cgcaccgcac
      541 gatccgcatg cgcgacggcc gcatcgcgag cgacgagtcg aacgggaagg gagagtaagt
      601 ggaagagctc ttcggcgtcc cggcgcagca actaatgtgg attctcctcg ccatcttcgg
      661 agcaggagcc ctgatcctcg gtctcgccgc cctccgcaac cgcgtctcct tcaggatggc
      721 ggcccgtaac ctgccgcgcc gccggaccca gaccatcctc gtcgtgctcg gcctgatgct
      781 cgcgactatg ctcttttccg cctcctttac cacgggagac accctgacca actccctccg
      841 cgtccagtcg ttggagaacc tgggccaggt cgacgttcag gtgcaggccg atggctcagg
      901 cgcctcaggc cagcagcagt tcggggacac atcctcagaa cgtgccagct acttcgatgc
      961 ggaggtggca ggcgaggtgc gcgaccggct ctctgacgag aatcacgtct cgggcgtggc
     1021 tcccgccgcc atcgagaccg ttcctgtaac ggccaggagc agcgacctga gcgaaccgag
     1081 cacggacgtg ctcggggtag acgaaagctc catgaagggc ttcgacaggc tcaccacttc
     1141 ctcgggcaag aagctcgacc cgaccgatct cggcaagaac gaaatctatc tgagcgccga
     1201 cgccgcgaaa ggactcgacg ttggggaagg ggatgtgatc gaggccacct tcgcccagag
     1261 atccggagct accgccgccc cgaacgcacc tccggccgct gccgcggttc ctggtgcttt
     1321 gcccgttccg cggccttcgg aactaaccgt ggctggcgtc tacgagagcg gggcgaatcc
     1381 ggcctcttcg acctcgatgg tcatgccatt gcagaggcta caggagcgga taggggagga
     1441 gggcgagatc aacgccgtcc tcatcaccca caagggccct gccatcgagg gtgccgcagg
     1501 cagcggcgcc accagggacg ccctcagagt gctcctcgag gagaacgacc tggaggccga
     1561 gcccgtcaag aaggaggcca tagatcaggc cgacgagggt ggggaaacct tcacgagcat
     1621 cttcctcctc ttcgggcaat tctctgtcgg ggcgggcgtg ctcttgatct tcttgatctt
     1681 cgtcatgctc gccgcggagc gcaaacacga gctcggtata gcgcgcgctg tcgggatgcg
     1741 cagggggcac ctgatgcgga tgttcgcctt cgagggggca ctctacgcct tgctcgcgag
     1801 cgccctcggg agcgtcgccg gcgtgggggt agggtggctg atggtgcgcg taatcggcaa
     1861 agcgttcgca ggctctggct tcgagatctc cttcgccaca aaccccgaga acgtggtcat
     1921 agcgttctgc ctcggcatgg tgctcacgtt cgcggtcgtc ctgatctcct cctggcgggt
     1981 gagccgcctg aacgtggtaa gggccatacg cgacataccg gagcctgaca ggaagggccg
     2041 cagtgtcaag ggcatcctct tcgcgcttac gaccccgctc gccggggcag gcctggtctg
     2101 gcagggcctt caggcggagc aaatgggcct gtacatgctg gggctctcgc tcatcatcgt
     2161 cgggaccgct ctcgcggccc gtgtgctgcg ggtgcccgaa cggatctcct tcacggtggc
     2221 agggacgctc ttgctcggac tctggctcct gcccttctcg ttcgcgccga gcggaatgac
     2281 agaaggcata gacctcttct ttatctcggg cgtaatgatc gtgctcgccg gcgtgtggat
     2341 cataatctac aactccgacc tgctcctcgg cgccgtcgtt gccctgttcg gttggctgcg
     2401 cggcatgccg cccgtcctca gggccgccgt gtcctacccg atgcacagcc gctttcgtac
     2461 cggcatgacc ctcgccatgt tctccctcgt cgtctttacc atcgtgacga tgagcttcat
     2521 aacggccgcc ttcggttcca tcttcgagga cacggaccgt ctctccggcg gcttcgacgt
     2581 gcgcgccgac gccggctacg cagcccccat cccggacatg aacgaggctc tgaaagataa
     2641 gaagagcgtg cacgccgccg acttcaccgc ggtcggcacc ctcaccgggc tctccgtgga
     2701 cgcgaagcag aaggacacga gccggaaacc gcagagcctg ttcttgcagg gagtggacgg
     2761 aggctacacc aagaatgtcg gctacgggtt caagtctacc gtcgccgagt acgacacagc
     2821 gaacgaagtt tggaccgcgc tgcggaccga gaaggacacc gcggttatct cgtcggatct
     2881 cgcacccagc aggtccaact ttgtcggcga tgaggaacct ccgataacac tctcgggctt
     2941 ctacggtgaa gacccaaagc tccccaatga cctctacata caggttgagg atcctcagtc
     3001 tgggaggacg cgaaacctgc acgtgatcgg ggtgctcgaa gacacggcgt tcttcgcgcc
     3061 gggcgtgacg tcctcgcggg cgaccgttga tgacctcgcg ggatctccct tgcctgcgca
     3121 gagctaccag ttcaggttgg cagacggggt gaattcggag gccgtcgcga aagacctgga
     3181 gaaagccttc gcccagaacg gccttcaggc cgtcgcgata aagaaagaga tcgaagacgg
     3241 ttcggcctcg cagggactct tcaacaacct gctcatgggt ttcatgggtc taggcctcct
     3301 ggtaggcatc gctgccctcg gcgtgatcgc ggcccgctcc gtggtcgaaa gaaggcagca
     3361 gatcggtatg ctccgggcgc ttggcttcca gcggggacag gtgcggctcg ctttcctcat
     3421 agagtcgtct ttcgtggccc tgctcggcat cgggctcggc gtggctttgg gcgccgcgct
     3481 ctcggtcggg atcgttgact cctttgagga gcagatctcc ggtatccgct acacggtgcc
     3541 atggagcacc ctgggcgtta tcgtgggcct cgcctacttc gcctcgctgc tcacgacctt
     3601 cctccctgcc agccaggcct ccagggtcta tcctgccgaa gcgttgcgtt acgagtaggg
     3661 aggcctgatt ctaagcgggg tacgtgcgga ccttcctttc ggcctcggga ccgagcatcg
     3721 ttgcatccgc gggccgatgc gctatatctc gcacggtact aaactggctt gagtcatgag
     3781 aataggcacg tccgatgatg ggtgagatct tcgcgctccg gcgattgcgg tggaagctga
     3841 cgctcagcta cacgctcgtg gccgcggtga cgctgctcgc gctggagttg atcctggccg
     3901 gcgcactggt cgccttcctg aactcgcctc tgttgccgag gctggtggtg cagaacttcc
     3961 gagacgaagt ggcgccgcgt ctcgagagac ccttgaacca gaatccgccg gacacggaag
     4021 ccatacgaga gagactagtg ggcttcgccg atagttccgg agtgcggggg gccgataggc
     4081 ccccgccgcc acccgtgagc ccgagcaatt acgcggcgga ccagggcgct ctcctcgtag
     4141 tcgacgacag acgccgcctg ctggtctccg tccctgagat gcaaggcttc cccgaaggca
     4201 ggcggttcga cgcgggcagc atctccggcc tcaagccgct ggtgaacgcc gcattcgacg
     4261 gcgaggagaa ctcaggccaa ctctcttcca acgtcgaggg ccaggtcgta accgtcacgc
     4321 ccatagaggg caagaagggc cgggtcgccg gggttcttgt cggcacgctg cggctgccga
     4381 acctcacggc gccgctcctc atcacggtgg ggatcggcgc gatcttcctg acgataccgg
     4441 ccgcctttct gggtgcgctc ttcggcttct ttaccgcctg gagcctgacc cgtcgcatag
     4501 ggcgactcgt ccgcgcagcc cggtcctgga gcaggggcga cttctccgtg acgacgaagg
     4561 accgctcgag ggatgagctc ggccagctct cgcgagagct caacagcatg gcggccgagc
     4621 tagaaaacct gctccaggcg cggggagagc ttgcgacgct agaggcccgc aaccgtttcg
     4681 cccgcgacct gcacgactcc gtcaaacagc aggtcttcgc gacctcgctc cagatcgccg
     4741 cggcccgcgc cctgatcgag aaggacgtgg gctcggctga gtctcacctc tcccaggcag
     4801 acgaactggt acgccaggcc cagaaagagc tcaacgtcct gatacaggag atgcgtcctg
     4861 cggccctgga agggaaaggc ctcgcggcgg cgctacgcga gtacgtcgtc cgctggtcgg
     4921 aaggagccga gatccgggct gattttcggg tccggggcga gcgtagggtg cctctggagg
     4981 tagaacaggc gctattccgc gtcgcccagg aggcgctggc caacgtcgcc aggcacagcg
     5041 gagccgggca cgctgaggtg gacctgtcct acgccacaga tgccatcacc ctgcgcgtcg
     5101 ccgacgacgg ccgcggcttc gacccctcgc ggaacccggg cggaggcttc ggtctgcaga
     5161 gcatgcgcga gcggctggta aggctcggcg gacacgtcaa cgtggagagc gcgcctggca
     5221 aggggacgct ggtggaatgc ttctgtccgg tacaggagaa gaaaggcgaa gacttatgag
     5281 cgagccgatc acggtcctgc tggtggacga tcacgcgctg gtgcggcagg gggtgcgcgc
     5341 ctttctgcag acgcaaggcg atatcagcgt ggtcgccgag gccgagagcg gggaggaggc
     5401 cgtgaggctc gccgcggagc acgcccccga cgtcgccctg atggacctca tcatgccagg
     5461 gatggacggc gtcgaggcga cccggcgact cgttgcgagc tcgccgcgca cgaacgtcgt
     5521 catgctaacc tcctaccacg acgacgagca catcttccct gctatccggg ccggcgccct
     5581 ctcctacgtc ctcaaggaga tcggcccgca cgaactcgcc gaagccgtcc gcaaggccgc
     5641 cgctggcgag gccgtgctgc acccgcgggt ggctgcacgc gtggtcaggg agctccacgg
     5701 agccaggcgc gccgagccaa acgtcttcca cgacctctcc gacagggagc tcgaggtgct
     5761 gaagttgatc gccgaaggac tgcacaactc ggagatcgca ggccgcctct acttgagcga
     5821 gaagactgtc aagagccacg tctcgaacat cctctccaag ctacacctcg cagaccgtac
     5881 ccaggccgca gtctacgcct ggcgccaggg cgtcgtacgg cgggactgaa gtcaggcttc
     5941 aggtgtcacg tgagaccgta ggccgatcgc gcaagtgcgt aggataacgc gaacactatc
     6001 tcaaagccct tccagaacct acggatcaac aagatggtac acatcgccta ctctagtcct
     6061 cagctctgtt cgtagagaaa agctgttgcc tgatgccggg ctgtgcggaa ggtcaatcct
     6121 tccgcgcagc cctacagcgt cgcgctcgtg ccgccggtac agacggcgcc tggctccggg
     6181 gtctgggggc cctgctggta ggcctggata aagctctcca ggtcggggtc gtccgctccg
     6241 ctcaggccta cttgcttgcc ccaggccgag gcaaccacag gggtgttact aggcaggtcg
     6301 ggataggggc tgaccaggac gaaggactgg ctctctgcga tgccacgcag ctcgtctacc
     6361 tggtcctgcg ggaggtctgg ggagtaggtg atccagaccg cgccgtgctc cagggagtgt
     6421 acggcactct cgtcgcggat tggcttgtca tagtagccgc agttctgcca gacagggttg
     6481 tgcgcccccc caacgggcgg actctgctcg tagtccacct cgcctccggt gtgctttccg
     6541 gccggaccca catcgtaggt ctttacgtcc tcagaagcgg cgccgccaga ctgctgtctg
     6601 gcgtcgagca ctgcgagggc gatgaaccct gctacgaaca cgacgataac cgcgcctact
     6661 atcaggtatg tccttctgga gccatccgac ccgctgcttc ttccgcttct ctttgccaaa
     6721 attttcctct ttctagtccg cggtgagatc gtagcacatg caacccgctt ctccgagagc
     6781 tttcagaacg cccgcgccac gagccatacg gtcaacgccg cgagggagat ccacacggcc
     6841 accactacca tggcagtcat cttgctgacc ggcctgtcct tcgcatcacg cgcctcttcg
     6901 cggcactcgg cgaggatttg gggtggaatc attctaatcg cgagcgcgac cccgagcggg
     6961 atgatgacga gatcgtcgag gtacccgagg acagggatcg gatctggtat caggtctatg
     7021 ggactgaagg cgtacccgac cacgaccgcg gcgaaagccc gcgcatacca cggcaccctc
     7081 gggtctttgt aggccagata cagcgcgtag acttcgacct tgagcctcag cgcccaccgc
     7141 ttgagtcgcc cgataggact caccgtcgct ggttggggtc cacggaattc atatttatcg
     7201 gcagcagtcg tcttcgcagt tctcgccagt gacgaccggc gcagtacagc agctctcgcc
     7261 gcgccacgac tcgcgaccct ctttcacggc aacgcccgcg atgaggagcg ccgtgacggg
     7321 gtcggcccac cacaggccga agagcgcgtt cgcgccgagc ccgacgagca gggccgcgga
     7381 gaggtaggcg cagagcatgt tctgctggcc ttcactcctc gtcgccgaag aaccgagttt
     7441 ctcgcctacg cgggccttgg cgatggcgag cgggggcatg gtcaccagcg tgacagcagc
     7501 gaggcctatg cccatccaac tggcctcagg ccgcgcgccg gagagtagac tcctcactgc
     7561 ctcgaagccg acgtaggccg ctatgaggta gaaacttacg ccgatgagct tctgggcgct
     7621 ccgctccgca ctctccgatg aggcgcgcgc ggcggcgaac cgccagagga ggatgacccc
     7681 tgctagcgat tcgatgagcg agtcggcccc gaaaccgatg agcgcgatgg acccggcgac
     7741 gaccccagcg ccgacagcga tcgccgcctc cacaccatgc cagcctacgc cgagccaggc
     7801 aaggagcttc gccctcctga ccagccgttc ccgctcgcct atccctggta gcttgcgcga
     7861 catctggagt ggctggccac tcacgagatc accttctctc ccaacacggc gtgcaggagc
     7921 gacctcccct cctccgtaag cgagtacatg accagtttgc cacttctccg ggatgtgacc
     7981 aagcggtgcg aacggagcag cctcagatgg tgggagacta ggttctgagc gcgagaggag
     8041 atccaggcca gatcacaaac acacagctcc tcaccttccc caagagccgc cgcaagcacg
     8101 agccgagtcg ggtccgagaa catcctcgcc cgttcagctg cttcccccgc agcttctatc
     8161 cctggcagcc ccacgcgtat cgcctcggcc cgcgggacgt caagacacaa tagatcgcag
     8221 cgctcatcgt tcatactttc attctaactc tcgttgatac gttggcacaa gtgggattag
     8281 tgtcacctgt agccttcctg tgcggaaggg ttcgaccttc cgcacggcgc tatcagtcga
     8341 ggcgggaggg gaagcggagg ttggtgtgat ggtcgcccac gtggtcgacc tggagggtag
     8401 tatgggagat cccgtactcg ttggtgagca gttcttcgag ttcgcgcctg cgggcgtggc
     8461 aatcttcgtg cctgccgacg agaacgtggg cggagagggc cgggaagccc gaggtgatag
     8521 tccagatgtg gaggtcgtgg acctcgatta tgccttcggc ggctgccatt ttctggccga
     8581 cttccccggc gttcaggccg cggggcgtcg cttcgagcag gatgttggtc gagtctctga
     8641 ggagtgtcca ggaactgccc aagatcagga acccgatggc cacgctgacc agggggtcgg
     8701 cgtagcgcca gccggtgagg atgataatgg cggcggcgac catcgcgccg accgaaccaa
     8761 gggcgtcggc gagtacgtgg cgcagggctc cctccacgtt caggctccct ctacctgacc
     8821 tgtagaggat ggtagcgccg gccgcgttga cgaggagccc gagcgtggcg acggccagca
     8881 tcggtgtgcc gaggacctca gccggctctc tgaggcgggc gtaggcttcc acgaagaccc
     8941 agatagagat cgccaccagc gtgatcccat tcgccagggc tgccaggatc tcggcccgac
     9001 ggtagccgaa actcttctcc ggtgtggccg gtctccctgc caaccaggct gcgaaaaggg
     9061 cgaccccgag cgagacgtta tccgagagca tgtgtcccgc gtcagcgagg agggccaggc
     9121 tccccgtgag gaggccgccg atgacctcca caaccgtgaa gctcgcggtc aggacgagca
     9181 ccaccgctag agcgcggcgg ttcccgtcac gcgcgttcgc tgtgtggtcg tgtccgtgat
     9241 ggcccatggg gctattctat cgcccgcggt actgacagcg ggtcaaggaa gggtcgaccc
     9301 ttcctactgc gacgtctcgc cgagcgtcac tgcgagcttc ttctcttgtg tgttgcggaa
     9361 gatcgtcagg atgaccctgt ctccaggctc atggtcgcgc agggcgccga ggaggtcccc
     9421 gtagcccact attctggcat tgtccagggc cgtgattatg tctcccttgc gcaccccggc
     9481 ctctctggct gggctacccg gtacgacctg caccaccagc gcgcccgaat ctatgggcag
     9541 gtcgaaccgt ccggcgtcct cggggctgag gtctgtcgtc aacacgccga ggtaaggagt
     9601 cgtgacctcc ccctcctcga taagctggtc tgccaccgag accgccgtat cagaaggtac
     9661 ggcgaagccg aggtctaccg ccccggtctc ggcaggaggt agataggcga cgttgatgcc
     9721 aatgatctcg ccgtcgcggt cggccagggc gccgccggag ttgcccagtg agatcgctgc
     9781 gtcggtctgg ataaggtcag tcagggcgtt ctgctccgcc tgacctccac tcgtcagctc
     9841 gggggggaac tcgcggccca caccgctcac cacgccggaa gttacggtcg actcgaagcc
     9901 agacgggcta cctatggcta ccgcgagctg gccgacgatg ggtgcgtctc catcgtcgaa
     9961 cgtggcagcc ggaaggtcgt tgcggttcac ccttatcacg gcgatctcgg tgttaggatc
    10021 cgtacctact atgcttgcct cttcgatggt gccgtcggcg aaggccacgt taacgccggt
    10081 ggccccttcg atgacgtggt tgttggtgac gatgtagccg tcggagcggt agatcacgcc
    10141 gctccctagc ccctcgcccc tctggatccc gagcggcgtc tctcgcaccg ccctgacgtt
    10201 gacctgcacg acgctgggcc ccaccttcga ggcgaccttg gccaccggct cgtcggccgg
    10261 gatggaagcc tgcggcgaga cattggccgt gttttcggct tgcgggcctg gctccgtctc
    10321 ggaacacccc gtaagcagga tcgtagtgag tatgaaggtg agcgaggcgg agatcgctgt
    10381 agatctcgat ttcaatgtag cagaacctcc tgtaggcgcc cggggcgcgt agttcccaca
    10441 gagtctatac gaggggcgtc cagaacggtt gtacgttgta ccctctaagg gtggatgcct
    10501 cgaccaacag gagagaggtg cccgccgacc tcgcccgcgc gggcgaggcc cgccacttta
    10561 tcgcctggag gggggccgtc gcggggctcg gcggcggaag gttgaacgag ttggccgtcg
    10621 ccgccgaagc cgtaatcacg gacatcctgc tctccgcacg agaaggtgta ctcgaaatac
    10681 agtccgtaca cgacgaggaa gtcgtcagaa tctctatcga acatcccgag atagagcagc
    10741 gccggatgaa ggatctagaa cgtgtgctgg agcggtatct cgacgattat gagattatcc
    10801 ctacccgcgc aatgctcgaa aagcggctgc gctagggggt tttcgagatc agccttccgc
    10861 agcgtccctg gaggcgggtt cgcggagggc ctccttgacc ttctgttctt gatctctgag
    10921 gtagaggaag acgtcctggg cgaagcgaac ggcgtgctct tcgggaaggt tgacccgcct
    10981 gtttccgagg accgactcaa gttccttgat ggctgcatcc gccatcccga ccatcgtctc
    11041 cgcctccctc aatgtctcct cgcgctccct gttcgagagg ctctggatgc ggttcag
//
LOCUS       DASTKM010000226         6489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_421043, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000226 DASTKM010000000
VERSION     DASTKM010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421043"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /gene="nuoK"
                     /locus_tag="VFQ10_09300"
     CDS             <1..365
                     /gene="nuoK"
                     /locus_tag="VFQ10_09300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET9965577.1"
                     /translation="GGGVVPPQVQQVVEALTATPPLDGYLVVGALMFAIGAWGALIRR
                     NAVVIFMCVELMINAVNLTLVAFADYLPNAGGLGVSYAVLVIAIAAAEIAVGLAIVLA
                     VFRSRRTVNVDEVTSMRG"
     gene            376..2358
                     /gene="nuoL"
                     /locus_tag="VFQ10_09305"
     CDS             376..2358
                     /gene="nuoL"
                     /locus_tag="VFQ10_09305"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9965578.1"
                     /translation="MQTIGQQLIITAIPTLPALVFLVLVLTGRLLKENAQYVAILGVT
                     ISLVFSVFALLFVAGIVPGGGAPIEFSVNWIDLGQGGSFPMGVYIDGLAAVMLVVVCF
                     VSLMIQLYSGAFMEGDKRFAWYYAMLNLFTASMLGLVLAPNFIQLYVFWELVGLCSYL
                     LVGHWFEKPSARDAAVKAFVVTRIGDAALFVGIIIFWATTGSTAFTDISEAAQAGFIG
                     GSLFTTAVILVFIGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYLVART
                     YDIFVQSPTAMLVVAYIGGFTALMAATMALVKKDIKRVIAYSTVSQLGYMMLGLGLGN
                     YTAGIFHLYNHAFFKALLFLCAGSIIYAMDSYNLFEMGGLRRRMPITFWTMLIAGLSL
                     SGIFPFAGFWSKDAIVASAYEEHYYLLFGMALLTVFLTAFYIFRAIFLAFTGEPRTEP
                     AREAAESPGIMTGPMMILAFFAIVSGWVGIPEGFGLPVRDYFSEFVRPSEFAASTLGL
                     EAHAFSFVLGGVSVVAGLLGIGLAYTLYVARPERAGELAARFSWLYTFLDKGWYFDAL
                     YGATFVRSAMWLGEATRRFDRNVIGGLVGGVGRGTLETGGLLQRLQSGGVQNYALFIL
                     LSVLIIGVIVGAQYAAIVVGLIALATIAAFAVGTRL"
     gene            2355..3809
                     /locus_tag="VFQ10_09310"
     CDS             2355..3809
                     /locus_tag="VFQ10_09310"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET9965579.1"
                     /translation="MITTITILIPLLGAILMLLLPDGERLVRYAALGVATVPLVLAVY
                     VYFVFGDDLGVAALTQDVTWIRSLNIGYRVGLGGLGFGMFFLSTFLTVIAVLASWDVR
                     SNLKQYFAALFVAEVGMLGVFAAQDLILFYLFFEVTLIPMYLLVGVWGDENRRPAAIK
                     FFIYTFLGSTVMLAGFLAFGILADTYAMNDLGGGGGLSRTAQVAVAAPILFGLLVKVP
                     AVPLHSWLLDVYVSSPTSTNVLLSGVLPKLGTYGLIAVAIPLLPQGVEPFLPYVAAFG
                     VINIIYGAFVAFLQPDLKALVAYSSIGTLGFILLGAASGNAVGLNGAVFQQVSHGLYS
                     ALLFILVGIIAARTGTRRIEDLGGLAARMPWAGGLLALGALAAMGLPGLAVFVSEFMS
                     IMGVYATFPVQGVLAALGIILSAMYLLYMLARVIFGPIERPAYEEIGDAGPAEMASVV
                     PLAVLLLVLGIFPALLIGIQRPAVDAVLSVIGGS"
     gene            3809..5239
                     /locus_tag="VFQ10_09315"
     CDS             3809..5239
                     /locus_tag="VFQ10_09315"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET9965580.1"
                     /translation="MTGQAFIALLPELISTGFLILVLMAGVFSDSNAGVAAGLAALGT
                     LAVFGSAALLLATGFSGSYFGGGFVVDGFALYFKLIVAGSAFFAVLAAARWSGQTGDA
                     PEYLTLILSVVLGSTLLVSMRDLFGVFLAIELATIPSYAMVAFDRSRRESAEGGMKYL
                     ITGVIASSVLLYGIVLIYGVSGSAMLQDVADTFSGNLTPVAVLGLVLMISGFAFKLSA
                     APFHFWTPDAYQGAPTSAAAFLSVGPKAATFAILLRILLEGMPDAAPTWTAVMAFLAI
                     ITMFVGNLFALRQRNVRRMLAYSSVAHSGYILAAFAAIQGGGVAFAVQAVLIYTAAYA
                     IMNMGAFLVIDLVGEDTKSYNGIVRTNPGVAASMALFMAALVGIPPLSGFFGKLWIII
                     AGAQSGSVLVYIVVGALVVNTVLSVPYYFGIIRNMIFEEPTTDTARAKGSGAVRFCVY
                     VLAVLTTLFGLIIIPLSALVRASGLV"
     gene            5314..6417
                     /locus_tag="VFQ10_09320"
     CDS             5314..6417
                     /locus_tag="VFQ10_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET9965581.1"
                     /translation="MQAVVLVGGQGTRLRPVTYDIPKSLVPLRNRPFMGYMLDFLRGA
                     GLDGAVLSLGYLPDPIQAYLSDKQDLVGFSVDYAVEDHALGTAGGIKNAEKYLDGGTL
                     VVVNGDVLTGMDLSRAIQAHKATDALATIVLTSVEDPTAYGLVEVDHEMLVHRFIEKP
                     AADEVTTNLVNAGVYVLEPEVLAMIPAGREVSIEREIFPELQESGRLRANITSSYWRD
                     IGTPRSYLAASHDVLSGAVGAGEGFEYLDVDPSVECGKNVRLLPPVSIAEECRIADLA
                     TIGGRSSLGKGSVVGEGAVVEGSILLDGAEVEAGAVVRGSVIGPRARVGNNSIVRGLS
                     VLGAGCVVGEGNVLDQGIRVNPGVVVSPRSLRF"
BASE COUNT          989 a         2086 c         2017 g         1397 t
ORIGIN      
        1 ggggtggagg agtagtgccg ccgcaggtgc agcaggtcgt agaggcgttg acggcgacgc
       61 cgccgctgga tggttatctc gtggtggggg cgctcatgtt cgccatcggg gcgtggggag
      121 ccctgatccg gcgcaacgcc gtcgtaatct tcatgtgcgt ggagcttatg atcaacgccg
      181 tcaatctcac gctcgtcgcc ttcgctgact acctcccgaa cgcaggagga ctcggcgtca
      241 gctacgccgt gctcgtgatc gctatagccg cggcggagat cgccgtgggg ctcgctatcg
      301 tgctcgccgt gttccgctcg cggcggaccg tcaacgtgga cgaagttacc tccatgaggg
      361 gctagaaggg aactcgtgca gaccataggc caacagttga tcatcacggc cataccaacg
      421 ctccccgcgc tggtcttcct cgtgctggtc ctcacaggac gcctcctcaa ggagaatgcg
      481 cagtacgttg ccatactcgg ggtgaccatc tcgctggtct tctcggtctt cgccctgttg
      541 ttcgtggccg gcatcgtccc cgggggcggg gcgcccatag agttctcggt gaactggata
      601 gatctcggcc agggcggttc gttcccgatg ggagtgtata tcgacggcct cgccgcggtg
      661 atgctcgtcg tggtgtgctt cgtgagcctc atgatccagc tctactcagg cgctttcatg
      721 gagggcgaca aacgcttcgc gtggtactac gcgatgctaa acctgttcac tgcctcgatg
      781 ctcgggctcg tgcttgcgcc gaacttcatc cagctctacg tcttctggga gctggtgggc
      841 ctttgctcct acctgctcgt cggacactgg ttcgagaaac cttccgcccg cgacgccgcg
      901 gtcaaggctt tcgtagtgac ccgaatcggt gacgccgcac ttttcgtcgg gatcatcatc
      961 ttctgggcta ccacaggctc gacggccttc acggacatct cggaggctgc ccaggccggg
     1021 tttatcggtg gctccctgtt caccacggcc gtgatactcg tctttatagg cgcggtcggc
     1081 aagagcgcgc agttcccgtt gcacgtgtgg ctccccgacg ctatggaggg acctactccc
     1141 gtgagcgccc tgatccacgc cgccaccatg gtcgctgcgg gcgtctacct cgtggcgcgc
     1201 acctacgaca tcttcgtcca gagccctacg gccatgctag tcgtggccta catcggcggc
     1261 tttacagcgc tgatggcggc gacaatggcg ctggtcaaaa aggacataaa gcgtgtgatc
     1321 gcctactcta ccgtctcgca gctcgggtat atgatgctag gcctcggact gggtaactat
     1381 acagccggca tcttccacct ctacaaccac gccttcttca aggccctcct gttcctttgc
     1441 gccggcagca tcatctacgc gatggactcc tacaacctct tcgagatggg tggactcagg
     1501 cgcaggatgc ccatcacgtt ctggaccatg ctcatagcgg ggctctcgct ctcggggatc
     1561 ttccccttcg ccggcttctg gtctaaagat gcgatcgtcg cctcggccta cgaggagcac
     1621 tactatctgc tcttcggcat ggcgctactg accgttttcc tcacagcgtt ctacatattc
     1681 cgcgccattt tcctcgcctt caccggcgag ccgcgcaccg agccggcccg cgaggctgcg
     1741 gagtcgccgg ggatcatgac gggtccgatg atgatcctgg ccttcttcgc catcgtgagc
     1801 ggctgggtgg ggataccgga agggttcggc ctccccgtaa gggactattt cagcgagttt
     1861 gtgcggccga gcgagttcgc tgcgtcgacg ctcggcctcg aagcgcacgc gttcagcttt
     1921 gtgctcggcg gggtttcggt cgtcgccggg ctactcggga ttgggctcgc ctacacgctc
     1981 tacgtcgcga ggcccgagcg ggcaggggag cttgcggcca gattctcctg gctgtacacg
     2041 ttcctggaca agggatggta cttcgacgcg ctctacggtg ctacgttcgt gaggtccgcg
     2101 atgtggctcg gagaggcgac ccgaagattc gaccgtaacg tcatcggtgg tctcgtgggc
     2161 ggggtgggac gcggcactct ggagacgggc gggctgctgc agcggctcca gagtggcggg
     2221 gtccagaact acgcgctctt catactgctg agcgtcctga tcatcggggt catagtaggg
     2281 gctcagtacg ctgcgatcgt agtcggtctg atcgccctgg caactatcgc cgccttcgca
     2341 gtggggaccc gactgtgata actacgataa ccatcctcat cccgctactc ggcgcgatac
     2401 taatgctgct cttgcccgac ggggagcgcc tggtgcgcta cgcggccctc ggggttgcga
     2461 ccgtgcctct agtcctcgcg gtctacgtct acttcgtctt cggggacgac ctcggcgtcg
     2521 ccgcgctcac ccaggatgtg acgtggatac ggtccctgaa tataggttac cgggtcggcc
     2581 tgggcggtct cgggttcggg atgttcttcc tctcgacgtt cctcacggtc atagcggttc
     2641 ttgcttcctg ggacgtgcgc agcaacctca agcagtactt cgccgccctc ttcgtcgccg
     2701 aggtagggat gcttggtgtc ttcgcggcgc aggatctgat cctgttctac ctcttcttcg
     2761 aggtcacgct gatccccatg tacctgctcg tcggtgtgtg gggggacgag aacaggcgtc
     2821 cggccgcgat caagttcttc atctatacgt tcctaggctc gacggtgatg ctggccggat
     2881 tcctggcttt cggtatcctc gctgatacct acgcgatgaa tgacctcggc ggtggtggcg
     2941 gcctctcgcg cacggcccag gtcgccgttg ctgcccccat tctcttcgga ttgctggtca
     3001 aggtgcccgc cgtgccgctg cacagctggc ttctggacgt atacgtctcc agcccgactt
     3061 cgaccaacgt gctgctctca ggggttctgc ccaagctagg tacctacggc ctgatcgcgg
     3121 ttgccatccc cctgcttccc cagggcgtcg aacctttcct cccttatgtc gccgctttcg
     3181 gggtgatcaa catcatctac ggggctttcg tagccttctt gcagccagac ctcaaggcgc
     3241 tcgtcgccta ctcctccata ggtactctgg gtttcatcct gctcggtgcc gcctcgggga
     3301 acgcggtcgg cctcaacggg gccgtcttcc agcaggtatc gcacgggctc tactccgccc
     3361 tccttttcat cctcgtcggc ataatagcgg cccgcacggg gacccgccgc atcgaggatc
     3421 tcggcgggct ggcagccagg atgccgtggg caggagggct cctggccctc ggtgccctcg
     3481 cagcgatggg gctgcctgga ctcgccgtct tcgtctctga gttcatgagt atcatgggcg
     3541 tgtacgccac gttccccgtg cagggagtac tcgcggcgct ggggatcata ctctcggcga
     3601 tgtacctact ctacatgctg gcgcgtgtga tcttcggccc catcgagcgt cccgcttacg
     3661 aggagatagg ggacgcgggg ccggccgaga tggcttcggt cgtgccgctg gctgtgttgc
     3721 tcctagtcct cggcatcttc ccggctctgc tgataggcat ccagcgcccg gccgtggatg
     3781 ccgtactgag cgtaataggg ggtagctagt gaccgggcag gcattcatcg cgctcttgcc
     3841 tgagctgatc tctacagggt tcctgatcct ggttctcatg gccggcgtgt tcagtgatag
     3901 caacgcaggg gtggctgcag gcctggccgc cctagggacg ctggccgtgt tcgggagcgc
     3961 cgcgttactg ctcgcgaccg gcttctcggg aagctacttc ggcggcggct tcgtggtcga
     4021 cggcttcgcg ctgtacttca agctgatagt cgcaggctcg gccttcttcg ccgtgctcgc
     4081 ggcggcgcgc tggtctggcc agaccggaga cgcgcctgaa tacctgacgc taatcctctc
     4141 cgtcgtgctc ggctctacgc tcctcgtctc catgcgggac ctcttcggcg tcttcctcgc
     4201 catagagttg gccaccatac cttcctacgc catggtcgcc ttcgacagga gccgccgcga
     4261 gagcgcagag ggcgggatga agtacctgat caccggcgtc atcgcctcct ccgtgctgct
     4321 ctacggtatc gtcttgatct acggcgtctc gggctctgcg atgctccagg acgtggccga
     4381 cacgttctcg ggcaacctta ctcccgtcgc ggtcctcggg ctcgtgctaa tgatctcggg
     4441 cttcgccttc aagctctcgg cggcgccctt ccatttctgg acccccgacg cctaccaggg
     4501 agccccgacg agcgcggccg ccttcctctc ggtaggcccc aaggctgcca ccttcgctat
     4561 cctcctgcgg attctcctcg aaggcatgcc ggacgccgcg ccgacctgga cggcggtgat
     4621 ggccttcttg gcgatcataa ctatgttcgt cggcaacctc ttcgccctca gacagcgtaa
     4681 cgtcaggcgg atgctcgcgt acagctcggt ggcccactcc gggtacatcc tggccgcctt
     4741 cgcggcgatc caggggggcg gcgtcgcgtt cgccgtgcag gcagtcctga tctacacggc
     4801 cgcctacgcc attatgaaca tgggtgcctt ccttgtcatt gatctcgtcg gcgaggatac
     4861 caagagctac aacgggatcg tcaggacgaa ccccggcgtg gcggccagca tggccctgtt
     4921 catggccgcg ctcgtcggca tacccccgct ctcggggttc ttcgggaaac tctggatcat
     4981 catcgcgggt gcccagtcgg gctcggtcct cgtctacatc gtcgtgggtg cgctcgtcgt
     5041 caacacagtc ctctccgtcc cttactactt cggcatcatc aggaacatga tcttcgagga
     5101 gccgacaaca gacacggcga gagccaaagg gagcggcgcc gtaaggttct gcgtctacgt
     5161 gctggccgtc cttaccaccc tgttcggcct gatcatcatc cctctctctg cgctggtcag
     5221 agcctcgggc ctggtgtgag gaacagagca ccgcacaaag atcgcctact tcgtattggt
     5281 cagagatccg tgcctggtgt aagctaggcc tctatgcaag cagtcgtact cgtcggtggg
     5341 caggggacca ggctgcggcc cgttacctac gacattccaa agtcgctggt gccgctccgc
     5401 aaccgtccct ttatggggta catgctggac tttctgcgcg gcgccggcct ggatggtgcg
     5461 gtgctctctc taggctacct gccagacccg atccaggcct acctctccga caagcaggat
     5521 ctcgttgggt tctctgtgga ttacgccgtc gaagatcacg ccctcgggac cgctggcggc
     5581 atcaagaacg ccgaaaagta cctcgacggc ggaacgctgg tggtcgtcaa cggcgatgtg
     5641 ctcacgggta tggacctctc cagggcgata caggcgcaca aagccacgga cgccctggcc
     5701 accattgttc tgacgagcgt agaggatccc acggcctacg gcctcgtcga ggtcgatcac
     5761 gagatgctgg tgcaccgctt tatcgagaag ccggccgccg acgaggtgac gacgaacctg
     5821 gtcaacgccg gcgtctacgt gctcgagccc gaggtcctcg ccatgatccc ggccggtcgc
     5881 gaggtctcga tagagcgtga gatcttcccc gagctccagg agagtggcag gctcagggcc
     5941 aacatcacaa gctcttactg gcgggatata gggacgccgc gcagctacct ggcagcctcc
     6001 cacgatgtcc tctccggcgc cgtcggtgcg ggtgagggtt tcgagtacct tgacgtcgac
     6061 ccctcggtcg agtgcggcaa gaacgtcagg ctcttgcctc cggtctcgat agccgaggaa
     6121 tgcaggatcg cggacctcgc caccataggc gggcgctcct cgctcggaaa gggcagcgtg
     6181 gtgggggagg gggctgtggt cgaggggagc atactccttg acggcgccga agtagaggct
     6241 ggcgctgtcg tgcggggttc tgtcataggc ccgcgcgcca gggtcggcaa caactccatc
     6301 gtgcgcggcc tctcggtgct cggcgccggt tgcgtggtcg gtgaggggaa cgtactcgac
     6361 cagggtatcc gcgtgaaccc cggcgtcgtc gtctcgcccc gcagcttgag gttctaacct
     6421 ccccaaccga caaccaggag ggacggccaa aggcccttgg aggagatccc ggtgtacgaa
     6481 ccttatctc
//
LOCUS       DASTKM010000227        15764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_69836, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000227 DASTKM010000000
VERSION     DASTKM010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 15764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15764
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69836"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            648..842
                     /locus_tag="VFQ10_09325"
     CDS             648..842
                     /locus_tag="VFQ10_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965582.1"
                     /translation="MRETGGEKARERNLRWMEALWEGTSTSAKEEDHKEEVLAVFRDL
                     VRESRSEREVREYVPWQPLW"
     gene            987..1691
                     /locus_tag="VFQ10_09330"
     CDS             987..1691
                     /locus_tag="VFQ10_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HET9965583.1"
                     /translation="MAQPIDLYYWPTPNGWKITTFLEEAGLPYNLIPVDITSGDQYES
                     EFLEISPNNKIPAIVDPEGPDGEPISLSESGAILIYLADKTGEFLPDVPRDRYLVLQW
                     LMFQMGHVGPMLGQAHHFRGYAPEKIPYAIERYTDEAARLYRVMDRRLSEAEYFAGEE
                     YTIADMAIFPWVVSHERQGQDLNDYPDLKRWYDAIESRSAVGRALEVGKELRRSLDEM
                     DDETRRTLFGGGPKRS"
     gene            complement(1699..2913)
                     /locus_tag="VFQ10_09335"
     CDS             complement(1699..2913)
                     /locus_tag="VFQ10_09335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9965584.1"
                     /translation="MKSLRVDGRLSFIVLLLSQLAATMGFTFVLPFTPIYVQALGVED
                     AGTAAAWAGVINSSTAVTMALAAPLWGRLGDRVGLKPMLLRATIAGSLVVGLMGLVGS
                     PWQLLALKTLQGCLTGTVAAATVLVSATAPPERAGERLGTLQMVIFVAAAAGPFFGGA
                     FADLVGIRASFGVTAALLAVSAVLISIWVKEERPEEKRSEESARAEGPLPHRRLAPTL
                     LALFVVQVAIMSAAPALPGFLSTLMEEPVRVATLAGWIIATGALAASLGSVIGGKLAA
                     RFGTRKVIIWALLLAGISALPQAGVDSIALLWILRLATGLFVGIAVPVANLAIRGAVH
                     PRRQGEAFGVAATATSAGNAVGPAAGGLLASSFGFGAPFLVPGLCLAAVSAIIWAAPL
                     LAVGYRTAVREH"
     gene            3412..3618
                     /locus_tag="VFQ10_09340"
     CDS             3412..3618
                     /locus_tag="VFQ10_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965585.1"
                     /translation="MGEFEIGVQYGTLNGGVYEDFIDFDFEGNDEMEDIFGEGQVALE
                     GERLIFELWPYHGDEWTFECERRR"
     gene            complement(3640..4671)
                     /locus_tag="VFQ10_09345"
     CDS             complement(3640..4671)
                     /locus_tag="VFQ10_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965586.1"
                     /translation="MAEEMPGIREWRGLFESAVRIKEISPWEWMTETDVFGVRSPETG
                     ELGFVSVMGLLGQHYAVSLYLGSEGIHGFLDLQEMGPFADPGALIQIPQLQASFENRE
                     ELDKRDRQVIKELGLKFRGRNAWPMFRSYRPSLFPWFLEAGEVRFLSCALEQLEDVAP
                     RFREDPSLLEPSGGESYLLRAPRQEGRRRRWEDASMGVPPLDAPPIEVEMEVPKIEAL
                     GRLPRGEVRLEVDFFMFPAPVQDEGDRPYFPHMLLVVDAGSGMVLGSDLLTPHPSLEA
                     MWGRVPENLADQLFAMELAPAKISVDSELLFDLLQPLASEAGFDLELSPSLPGLESAR
                     ENLLHTFGE"
     gene            5027..5575
                     /locus_tag="VFQ10_09350"
     CDS             5027..5575
                     /locus_tag="VFQ10_09350"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET9965587.1"
                     /translation="MGHSNHSAEKFTVLLRRHGIEVLVDIRSRPYSRHAPQFNATAIE
                     ATLSGNGIRYLFLGRELGGRPVEETFYDTKGRVDYALVERSRPFLDGIKSVEREIQSN
                     RISLLCSEEDPARCHRRLLVGRALEERGFTLRHIRGDGSLQIEGETEDGQPVLFPERE
                     ASPRKSIRSVSRKRRRPSSSER"
     gene            5572..5949
                     /locus_tag="VFQ10_09355"
     CDS             5572..5949
                     /locus_tag="VFQ10_09355"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET9965588.1"
                     /translation="MKDTGIERLLDVRLNNASRFSGFARRADLPFFLERLCGAEYVHE
                     PRLAPTREILDAYRKGGRWPDYERAFLELLRERRVEEALDRDLFAVPTVLLCSEANAE
                     HCHRRLVLEYLDREWNGIRAVHL"
     gene            5955..6737
                     /locus_tag="VFQ10_09360"
     CDS             5955..6737
                     /locus_tag="VFQ10_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965589.1"
                     /translation="MQILVNHLTRMQPGYFCVAGVDVNTPRHIRPVLRRGRLTLDLLS
                     TGGGPFDVGSVVDLGAATFAGRAPEMEDHRFDPSSTQWLFDEDPDDYWNALDGVAQEN
                     LAEIFGSALELWDESGTVDVGEGRASLGCLKPEKQPWLYVDHRGTVRMVLDYLMPSVD
                     LSVNDLRLYERDGRTPRRDLVASVERRLKSGVEAILSVGLTRPWQKWGDTAERHWLQV
                     NNIHLKDDPLWQLRGQRRTGQATEDLARSRLERREEKSTLTP"
     gene            complement(6766..8340)
                     /locus_tag="VFQ10_09365"
     CDS             complement(6766..8340)
                     /locus_tag="VFQ10_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET9965590.1"
                     /translation="MRPGITTILEKNSPPMWFYAGDRFHYEKLPEGTRVIYPPGPSKP
                     LEEPGVAIERALLEPMDMEPLHELLRPGMKLTIAFDDLSIPLPPMRKPDVRQQVIEKV
                     LEKAAARGVDDIHLIAALGLHRRMTEAELRHCLGGRIMSRYYPDRLYNYDAEDPDGNV
                     RLAETDLGEEVSVSRRVAESDLLVYVNVNFIPMDGGHKSVHTGLAPYSSIRHHHTPKT
                     LANTRSLMDPPNSAMHASINRMGRIFDEHVKVFHIESTLNNQTYPAAFDFMAKPEYQW
                     SMLTKANFLATHKATQTMPLEAARRISHSINAPHRVTSVQAGATEPVHAATLSDNYRQ
                     MAVPAEGQSDVLLLGIPYLGPYNVNSIMNPILVFNMLLGYLFNLYKGKPLVREGGVLI
                     GTHPMPANFNKLHHPSYIDLWNEVFPETNDVHEIHHRYEEKYANDPWYRTLYRNSYAY
                     HGAHPFTVLYWAAHALDHLGGVILVGGDPENTKRMGLGRADTVADALQMAQDTVGPNP
                     STTYMHIPPLFLCEVS"
     gene            complement(8527..9729)
                     /locus_tag="VFQ10_09370"
     CDS             complement(8527..9729)
                     /locus_tag="VFQ10_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9965591.1"
                     /translation="MQALVYRKSIPRYVLMRAGSKRIKSLETSRFSPLQLEDAPDFGL
                     PSPQWVRVKPLLSGICGSDLGTLSSENSPYFSPITSPPFVMGHEVVGEVVEDNSGFRA
                     GERVVLEPALGCAVRGIAPPCPYCASGRHALCLNVARGDISPGIQTGFCCDTGGGWSQ
                     GTLVAHPSQLHRVPDAVSDESAVAIEPLACAVHAALGAAPGDTTLVIGAGSVGLFTVA
                     ALRHLTDAGRIICVAKHERQRNEALRLGATEVVHPKETYTKLPEMLGTTAYKPELGKP
                     VVMGGAGKVFECVGAPGTLEDALRLAAPGAEVTLVGMPGAKSTLDLTALWYKEVSLAG
                     SYAYSVENYQGEKTSSFQLALSIAPKIELQTLVGPSFQLDSYKEAIAAARAAGREGHI
                     KVVFDHRS"
     gene            complement(9731..11998)
                     /locus_tag="VFQ10_09375"
     CDS             complement(9731..11998)
                     /locus_tag="VFQ10_09375"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET9965592.1"
                     /translation="MLGEAYKDKKILLTGGTGFLGTALVEKILRSLPNLGRLYLVIRS
                     SRGKSAAERFERDVLGAAALRGLREKLGHDFEDHVSKKVRVLEGDVHAPSLGLGEEDL
                     TELSREVDVVIHSAASVIFDAPLDAAIDSNVTGTLGLLQLARAWEKLPLFIHISTAYV
                     AGITKEDAPEAPPGPSSPNGTTLDAREEVLGLEAVVAEVDEASRERSLLRRFETEARR
                     ELGMVGEDEEVAERVDQLRRAWMRERLVERGTRRARELGWNDVYTFTKSLAERIVVAE
                     RGETPLVIVRPAIIESSHREPYPGWIQGTRMADPIIMAFAKGILREFPGNPKSYVDIV
                     PVDHVVNAILAAGIRRPEEPEIFQVASGERNPIRYRDLYDIVRDYFVQNPLRDSSGRP
                     IQVPEWSFPGRRRVEGQLKVELAALKVAGLLAARMPEGHMASDARQRIARAEKRAKMS
                     LYYSHIYGAYANMTSTFASSRTEGLYTALPPEDREEFPFDIADIDWRAWMQGAHLPAL
                     TTRPSRKKRKKVTAEKPGEVAAIFDVDGTIVGSNVVSYFAWLRMRELPVTLRPLWLAG
                     FLTKVPYYWGLDKVSRAHFNRAFYKNYAGWKPSRARHLGQESFAGFTLDRIFPDALQQ
                     LRDHKAAGHRVVLLSGALDFLLEPMKDLADDVLCSTLAQENGAYTGELTGAPVAGDAR
                     ARMLASFARRRGLDLTRSYAYADSISDLPMLEAVGNPVAVNPDRRLRTAAKARGWKVE
                     HWDKNGVADGMAKKI"
     gene            complement(11999..12598)
                     /gene="plsY"
                     /locus_tag="VFQ10_09380"
     CDS             complement(11999..12598)
                     /gene="plsY"
                     /locus_tag="VFQ10_09380"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET9965593.1"
                     /translation="MLGLLIVLFGYVLGSVPTGYLVGRALGVDVRKVGSGNIGTANVL
                     RAAGKWAAILTLAGDMLKGLVPVVVARLAVQNEWLIAAVAFAALIGHCWPVFLRFRGG
                     KAVATGAGTTIGLAPLVGLCLFAFWWAVVLLSRYTSLGAIAVMVISPVVFWLSGQPLP
                     YVLYTIIGGALVLWRHRENARALLNGTERKVGQRAEEGS"
     gene            complement(12642..14159)
                     /gene="trpE"
                     /locus_tag="VFQ10_09385"
     CDS             complement(12642..14159)
                     /gene="trpE"
                     /locus_tag="VFQ10_09385"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET9965594.1"
                     /translation="MTATANPGLVPSLGEARRLARSYDVVPLYAEFIGDLETPISAVL
                     RFAEEDTVFLLESAESAERFGRYSFLGFDPKRTLSYRDGLYTVVDADGVREVPARDPF
                     RGLAEIVGKKSVAPLPNLPAFVGGAVGYFSYDAVRYLERLPDAPGDDLDVPEACFAVT
                     DTLIVFDHLRHKVLVVSLIDAAGLRDVEGEGFSAAYRRAADDVRRIVERLSAPLVRRT
                     PAFGTGVEISSNFTREGYEEAVEAAKEYIRAGDAFQIVPSQRFAADIGDLDPLLLYRG
                     LRTVNPSPYMTYLKLGGLSIVGASPEPLVRVEGRRVMTRPVAGTRGRGGTPEEDAALA
                     EELLADEKERAEHVMLVDLGRNDLGRVSEVGSVGLESFMEIERYSHVMHIVSTVEGDL
                     REDLTALDALAAAFPAGTVSGAPKVRAMEIIDELESTRRGPYAGATGYYGVDGRLDTC
                     ITLRTALLKDGVAYFQAGGGVVADSVPSLEYEETRNKARAMEQALEVARTREMWL"
     gene            complement(14143..14784)
                     /gene="hisIE"
                     /locus_tag="VFQ10_09390"
     CDS             complement(14143..14784)
                     /gene="hisIE"
                     /locus_tag="VFQ10_09390"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HET9965595.1"
                     /translation="MTKATDRVKYDQNGLVPVVAQDAATGDVLTLAYANREAFEKTLS
                     SGEAHYYSRSRSELWRKGARSGNTQRVVEVKLDCDGDALLYRVEPRGPACHTGQKSCF
                     FTTLAGERIGIASAGDGEIPFGTIVERLAGTIARRHREMPEGSYTVSLIQGGKERLAQ
                     KVGEEAVEVVVAALSDERLSEEAADLVYHLLVLLEERGVGIEDVAKVLHDRHG"
     gene            complement(14781..15578)
                     /gene="hisF"
                     /locus_tag="VFQ10_09395"
     CDS             complement(14781..15578)
                     /gene="hisF"
                     /locus_tag="VFQ10_09395"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET9965596.1"
                     /translation="MSLKVRIIPCLDVDAGRVVKGTNFTNLRDAGDPVELAALYDREG
                     ADEIVFYDITASHEQRDTAAALARAAAEEVFVPYTIGGGVRTAGDMRAMLRAGADKVS
                     INSAAVGNPDLITEGAERFGSQCVVLSVDVKRRGLSPGWEVYLNGGRVDTGMDAVGWL
                     IEGERRGAGEFVLNSMDADGTETGYDLDLISAVAEKTTLPIVASGGAGSPEHMIAAVN
                     AGAGAVLAASIFHFGEYSIAEVKEHMMQAGIPVRLVNQVSPRDEIEE"
BASE COUNT         2895 a         4901 c         5061 g         2907 t
ORIGIN      
        1 gttcctgcaa atggagcctt tatttgctct tgtgtccctg tggggtggcc tgaccctcca
       61 gaggtgattg cagtccctta gaatggatcc tcgtgcgtct ggtggggtgc atatcactag
      121 agatctctta ggaatggcag ctagagctcg ctgcggtggg ggcaacgctt gccttgcggg
      181 tgatctctct gctcgtctat ctctacgcag cttaacgcat ctagagagaa gcgtgacgat
      241 tgatggctac accaaagtta tctccctgtg atctgcccta attctgatga ttagggtgtt
      301 agtcaaattg tcagtgcttg ccgatcccca cctgcaagaa tacccccagg ggagggagaa
      361 gtatcgcgat aatgatgcgt aggagatcca tcgtaagcct ctttctgttg ctcgctctct
      421 acctgatacg ctgacccacc ctggtagttg caggcaccga gagtatccta caacgccgcg
      481 cgtaaggcta tagccctacg cacgaaacac caacatgttg gggccatttc cgaggtgtat
      541 gatacataac gtgtttcatg catggatctg tatgaactgc taggctgaag cggacgcatc
      601 gctcatctta gggtaggaac cgtgtcaccg gaggagggag tctaggcatg agagagaccg
      661 gcggagagaa ggccagggaa aggaacctac gctggatgga ggcgctgtgg gagggcacga
      721 gcacgtccgc caaggaggag gaccataaag aagaggtact tgcggtgttt cgtgacctgg
      781 tgagggagtc caggagcgag agagaggttc gcgagtatgt gccctggcaa ccgctgtggt
      841 agcagctggg tcccctacat gacgtctggc tgacggtggt gctcctccgc tgcgcggcct
      901 gaaggtaggc tgcttctcct atactatgtc ctcacggaag gactatacct ctggtagggt
      961 acccaccagg agcgaggaga agccgaatgg cgcagccgat cgatctctac tactggccga
     1021 cccccaacgg ctggaagatt acgacctttc tcgaagaagc agggcttcct tacaacttga
     1081 tacccgtgga cataacgtcg ggcgaccagt acgagtcgga gtttctggag ataagcccga
     1141 acaacaagat acctgccatc gtagaccctg aaggtcctga cggagagcct atatcactct
     1201 ccgagtcggg agccatcctg atctacctcg ctgacaaaac gggtgagttc ctacccgatg
     1261 ttccacgcga caggtacctg gtgctgcagt ggctgatgtt ccagatggga cacgtggggc
     1321 cgatgcttgg gcaggcacac cacttcagag gctacgctcc cgagaagata ccctatgcaa
     1381 tagagcgcta cacggacgag gccgccaggc tctacagggt catggaccgg cggctctccg
     1441 aggcagagta cttcgctggc gaagagtaca cgatcgctga tatggcgatc ttcccatggg
     1501 tcgtctcgca cgagaggcag ggccaggacc tgaatgacta ccctgacctc aaacgctggt
     1561 acgatgccat tgagtcgcgc tccgccgtcg gccgcgcgct ggaggtcggt aaggagctac
     1621 gccgctcgct ggatgaaatg gacgacgaga cccgcaggac gctgttcgga ggcggcccga
     1681 agaggagtta gaggcgctct agtgctcacg cactgcggtg cgatatccga cggcgagcag
     1741 gggcgctgcc cagatgatcg cggagacggc ggccaggcag agcccaggga ccaggaaagg
     1801 cgcgccgaag ccgaacgaag aggctagcag accaccggcc gctggcccga cggcgttgcc
     1861 tgccgacgtg gccgtcgctg cgaccccgaa cgcctcgccc tggcgcctgg ggtgtacggc
     1921 gccgcggatc gcgaggttgg ctacggggac ggcgatcccg acgaacaggc ccgtcgccag
     1981 gcgcaggatc cacagcaggg cgatggagtc gaccccggcc tgcggcagcg ccgagatgcc
     2041 tgccagcaga agggcccaga tgatgacctt ccgtgtcccg aacctcgccg cgagctttcc
     2101 tcctatgacg gagccgagcg aggcagccag ggcgccggtc gcgatgatcc aacctgcaag
     2161 tgttgcgacc cggacaggct cttccatcaa ggtcgagagg aaaccgggta gcgcaggcgc
     2221 tgcgctcatg atcgccacct gcaccacgaa gagcgccagg agggtcggcg cgagccgcct
     2281 gtgcggcagc ggaccttcgg ctcgggcgga ttcttccgac ctcttttcct caggccgctc
     2341 ttccttgacc cagatggaga tcaagactgc tgacaccgcc aggagagcag cggtcacccc
     2401 gaaagaggcc cttatgccga cgaggtcggc gaatgctcca ccgaagaacg gcccggccgc
     2461 agcggcgacg aagatgacca tctgcagggt tcccagcctc tcccccgccc gctccggcgg
     2521 ggccgtagcc gatacgagga ccgtcgccgc agcgaccgta ccggtcaggc atccctgcaa
     2581 agtcttgagg gccagcaact gccacgggct tcccacgagc cccatcaacc cgacgacgag
     2641 agagcccgca atcgtcgccc taagtaacat aggcttgaga ccaacacggt cgcccagcct
     2701 gccccacaac ggcgcggcca gggccatcgt cacggcggtg gagctgttga tcaccccggc
     2761 ccacgccgcc gctgtaccag cgtcctcgac gccgagcgcc tggacgtaga tcggggtgaa
     2821 aggcagaacg aacgtgaacc ccatcgtcgc agcgagctgc gagagtagca gcacgatgaa
     2881 agatagcctt ccgtcgaccc gcaaagactt cattcgccgt ccctttccgc aaggcagcgg
     2941 acgccaggga cggcgggatc tcctcctgca gatgtgcctc tttgcctggc cgccaaaagg
     3001 ggcggcccta taaccctgtg tagcgtaact ctccttcccg gatactccgg ggctcgtgag
     3061 gcgcggtgaa cgccgccaga cagttgcgac cctacaggcc gcgcaaatga gggaggctac
     3121 cggcgttctg aaaccagcgc ctaatatcct cccgaccacg ctcgaaaact ctcacgccgg
     3181 atatacgtct ctcaaaacct tcgctaccaa gtgtagcaaa cgccctgtcg caaatccgac
     3241 cttgtaactg tctgaggcac caccggagtg aaagaattca cgccagatag aatgtgggaa
     3301 atggatttca acggtacttg gcaagtcgtt tcgagcccgg atttcgaaga tgattacctg
     3361 aacatgcggg ttcccgctca cgttactctc cggcagaatg gagacttcgt aatgggtgag
     3421 tttgagatag gggtacagta cggaacgttg aacggaggcg tctacgagga tttcatagac
     3481 ttcgatttcg aggggaacga cgagatggaa gatatcttcg gcgaaggtca ggtcgcgctg
     3541 gaaggggaac gcctgatctt cgagctctgg ccctatcacg gcgacgagtg gaccttcgaa
     3601 tgcgagcggc ggaggtagaa gctctctcag cccgcgagct cattcgccaa atgtgtggag
     3661 caggttttcc ctagcagatt ccaggccagg gagggacggc gacagttcca ggtcgaagcc
     3721 agcttcacta gccagtggct gcagaaggtc gaacagcagc tccgaatcca ccgagatctt
     3781 tgcgggcgcc agctccattg cgaagagttg atcggcgagg ttctcaggga cccggcccca
     3841 cattgcctcc agggaggggt gcggcgtgag caggtcgctg cccaggacca tgccgctccc
     3901 ggcgtctact accagcagca tgtgcgggaa gtacggacga tccccctcat cctggacggg
     3961 cgcggggaac atgaagaagt ctacctccaa gcgcacttcc cctcgcggca gccgtccgag
     4021 agcctcgatc ttgggcacct ccatctcgac ctcgatgggc ggagcgtcca gcggaggcac
     4081 acccatagaa gcgtcttccc agcgacgcct cctcccttcc tggcgcgggg cgcgaagcag
     4141 gtagctctcg ccgccggaag gctccagcaa agaagggtcc tctctgaagc gcggagccac
     4201 gtcttccagc tgctccagcg cacacgatag aaaacggacc tctccggcct ccaaaaacca
     4261 cgggaagagg gacgggcggt agctccggaa catcggccag gcgtttcgcc ccctgaactt
     4321 caagcccagt tccttgatga cctggcggtc tctcttgtcc agctcctcgc ggttttcgaa
     4381 cgatgcctgg agctggggga tctggatcag agcccccggg tccgcgaagg gtcccatctc
     4441 ctgtaggtcc agaaaaccgt ggatgccctc ggagccgagg tagaggctca cggcgtaatg
     4501 ctggccaagt aatcccatca cgctgacgaa tcccagctcg cccgtctcgg gactccgcac
     4561 gccgaagacg tccgtctcgg tcatccactc ccagggtgag atctccttga tccgaacggc
     4621 cgactcgaag agcccgcgcc attcccgaat ccccggcatc tcctcagcca tgcgtcctcc
     4681 cgcatcaatg ctagacgcct gtgattctag actacaggct ccgcgcctcc aatgacgaag
     4741 ccaacccggt gtgcaaatcc agcgaaagaa taggctggag aaggtccttc aactcctgca
     4801 gagcaacaca ggccaggagg cttcctcgcg gtggtgcaag ctatctcgaa agaaggtctg
     4861 gggagagcgc ggccgtgcga ttatcctaaa tgtccgatga ttcccgggaa cgcgttgcgg
     4921 gaggataggg actgttgatg ctgtataccc aaaggctcgc caagacggag gatgattagc
     4981 catcggcgat aggtggtcgg cggagagccg gggatcggtt ttcacaatcg ggcattccaa
     5041 ccattcggcg gagaaattca ccgtcctgtt gaggaggcac gggatcgagg ttctggtgga
     5101 catccgctct cgtccatact ccaggcatgc accgcagttc aacgccacag ccatcgaagc
     5161 gaccctgtcg ggcaatggga tccgatacct attcctgggc agggagctgg gcggaagacc
     5221 cgtagaagag acgttctacg acacgaaggg ccgggtagac tacgcgctgg tcgaaagatc
     5281 ccgaccattc ctcgatggta taaagagtgt ggagagggag atccagagca acaggatctc
     5341 cctgctatgc agcgaggaag accccgcccg ttgccaccgc aggttactcg tcgggcgggc
     5401 tctggaggag cgagggttca cgctgcggca catcaggggc gacggtagcc ttcagataga
     5461 gggcgagacg gaggacggcc agccggtcct gttcccggag agggaggcga gcccacggaa
     5521 gtctatacga tcggtttcac gaaaaagacg gcgcccgagt tcttcggagc gttgaaggac
     5581 accggcatcg agcgcctcct cgacgtacgg ctgaacaacg cgtccaggtt ctccgggttc
     5641 gcccgacgtg ccgaccttcc tttcttcctg gagaggctct gcggcgcgga gtacgttcac
     5701 gagcccagac tggccccgac acgcgagatc ctcgacgcct acagaaaggg cggcagatgg
     5761 cccgactacg agagggcgtt tctggaactc ctgcgggagc gccgtgtgga ggaggcgttg
     5821 gatagagacc tcttcgcagt tccgaccgtg ctgctttgca gcgaggccaa tgccgagcat
     5881 tgccaccggc gtctggtcct cgagtacctg gaccgggaat ggaacggcat cagagccgtt
     5941 cacctgtagg gaacttgcag atactcgtaa accatctgac gcggatgcag ccaggctact
     6001 tttgcgtggc aggcgtggat gtgaacacgc cgcgccacat ccgtccggtg ctccgccgcg
     6061 gcaggctcac ccttgatctc ctcagtaccg gcggcggacc cttcgacgtc gggtccgtcg
     6121 tggacttggg ggcggcgacc ttcgccggac gagcccccga aatggaggac catcgcttcg
     6181 atccctcgag cacgcaatgg ctcttcgacg aggaccctga cgactactgg aacgccctcg
     6241 acggggtggc acaggagaac ctggcggaga tcttcgggtc cgcgctcgag ctttgggacg
     6301 aaagcggcac ggtggacgtt ggggaaggac gcgcctcact gggctgcctg aagccggaga
     6361 aacaaccgtg gctgtacgtc gaccaccgcg gcacggttag gatggtgctc gactacctga
     6421 tgccctccgt ggacctctcg gtgaacgacc tgcggctgta cgaaagggac ggacgaacac
     6481 cacgccggga cctcgttgcc agcgtggaaa ggcgcctcaa gtcaggcgtc gaggcgatcc
     6541 tgagcgtcgg cctgacgcga ccctggcaga agtggggaga caccgccgag cgacactggt
     6601 tgcaggtcaa caacatccac ctcaaggacg acccgctgtg gcagttgagg ggccaaaggc
     6661 gaacggggca ggccaccgaa gatctcgcaa gatctcgcct cgagcgccgc gaggagaaga
     6721 gcacgctgac accgtagtcg tgcggagccc gttacgaggc agaccctagc tcacctcgca
     6781 caagaagagg ggcgggatgt gcatgtaggt cgtgctcggg ttcggtccaa cggtgtcctg
     6841 ggccatctgt agcgcatcgg ctacggtgtc ggcccgtccg agtcccatac gcttcgtgtt
     6901 ctccgggtcg ccgcctacca gtatgacgcc gccgaggtgg tcgagggcgt gggctgccca
     6961 gtagaggacc gtgaaagggt gcgcgccgtg gtaggcgtag gaatttcggt agagggtacg
     7021 gtaccagggg tcgttggcgt acttctcctc gtagcggtgg tggatctcgt ggacgtcgtt
     7081 cgtctcgggg aagacctcgt tccacaagtc tatgtagctc gggtggtgga gcttgttgaa
     7141 gttggccggc atggggtggg taccgatcag gactcctccc tcgcgcacga gcggtttgcc
     7201 cttgtagagg ttgaagaggt agccgaggag catgttgaag accaggatag ggttcatgat
     7261 ggagttgacg ttgtagggtc cgaggtatgg gatgcccaac aggagaacgt ccgactggcc
     7321 ctcggcgggg acggccatct gcctgtagtt gtcgctcagt gtcgctgcgt ggacgggctc
     7381 tgtggcgcct gcctgtacac tggtaacccg gtgcggagcg tttatggagt gagagatgcg
     7441 cctggcggct tccagcggca tcgtctgcgt ggccttgtgg gtagcgagga agttggcttt
     7501 ggtgagcatg ctccactggt actcaggttt cgccatgaag tcgaaagctg cggggtaggt
     7561 ctgattgttc agggtgctct ctatgtggaa gaccttgacg tgctcgtcga agatcctacc
     7621 catccggttt atggaggcgt gcatggctga gttcggtggg tccatcaggg agcgggtgtt
     7681 ggccagcgtc ttcggggtgt ggtgatggcg gatggaggag tagggggcca ggcctgtgtg
     7741 cacggacttg tggcccccgt ccatggggat gaagttcacg ttcacgtaga cgagcaggtc
     7801 actctcggcg acgcggcggc ttacgctgac ctcttcgccc aggtctgtct cggccagacg
     7861 tacgttcccg tcagggtcct cggcgtcgta gttatagagc cgatcagggt agtagcggga
     7921 catgatcctg ccccccaggc aatgcctgag ctcggcttcc gtcatcctgc ggtggagacc
     7981 gagggccgcg atgaggtgga tgtcatcgac acctctggcg gcggccttct ccagtacctt
     8041 ctcgatgacc tgctgacgaa cgtcaggctt gcgcatcggt ggcaacggta tcgagaggtc
     8101 gtcgaaggcg atggtcagct tcatgcctgg cctgaggagt tcgtgcagcg gctccatgtc
     8161 catcggctcg agtagtgcgc gttcaatggc gacgcctggc tcctcgagag gtttcgaagg
     8221 tccagggggg tagatcacac gcgtcccctc aggcagcttc tcgtagtgga acctgtcccc
     8281 cgcgtagaac cacatcgggg ggctgttctt ctccagaatg gtcgtgatgc cgggtcgcat
     8341 tgggctccct tcggtcgcag cagttcagca cgcttcgggc cttcggccct cgcttgtcag
     8401 catttcggct ttttggtggc ggttcgccgc tgacggtaga ccgcgcatcg ctctcttcac
     8461 aaaccgcacc tccatacgat ctatctcctc gccgtccgtt gttaagctga gtagctgaag
     8521 tgctgtctag ctcctatggt cgaagactac tttgatgtgt ccttcacgtc ctgctgcgcg
     8581 cgcggctgcg atggcctctt tgtaggagtc cagttggaag ctgggaccta cgagcgtctg
     8641 tagttctatc ttgggggcga tgctgagtgc gagttggaag ctgctcgtct tctctccctg
     8701 gtagttctcg acactgtatg cgtagcttcc ggcgaggctg acctctttgt accaaagggc
     8761 ggtgaggtcc agagtgctct tcgcgccggg catgccgacg agcgtgacct cagcgccagg
     8821 cgcggccagg cggagtgcgt cctccaaagt acctggcgcg ccgacgcatt cgaagacctt
     8881 gccggctccg cccatgacga ccggcttacc gagctcgggc ttgtatgcag tcgtgcctag
     8941 catctcgggg agtttcgtgt aggtctcttt ggggtggact acctcggtcg ccccaagacg
     9001 aagcgcctcg ttcctctgtc gctcgtgctt ggcgacgcag attatgcggc ctgcatcggt
     9061 gaggtgtctc agggccgcga cggtaaagag gccgacgctg ccggccccga tgaccagggt
     9121 ggtgtcgcca ggggcggccc cgagcgcggc gtggacggcg caggccaggg gctctatggc
     9181 gacggcggat tcgtcggata ctgcgtcggg cacgcgatgt agctgcgagg ggtgagcgac
     9241 gagcgtgccc tgtgaccagc caccgccggt gtcgcagcag aagcccgtct ggatgccagg
     9301 gcttatgtct cccctggcca cgttaaggca cagggcgtgg cggcccgagg cgcagtaggg
     9361 gcaagggggg gctatgccgc gaaccgcgca tccgagggcg ggctcaagga cgacccgctc
     9421 gccggcgcgg aagccggagt tgtcttcgac tacctcaccg acgacctcgt ggcccatcac
     9481 gaagggaggg gaggtaatcg gggagaagta aggcgagttt tccgaggata gcgttcccag
     9541 gtccgagccg cagataccgg agaggagcgg ctttacgcgt acccattgtg ggctcgggag
     9601 accgaagtca ggggcgtctt cgagttggag cggagagaag cggctcgttt cgagggactt
     9661 tatccgcttc gagccggcgc gcatcaagac gtagcgtggg atggactttc ggtagacgag
     9721 ggcttgcaac ctagatcttc ttggccatac cgtccgcgac gccattcttg tcccagtgct
     9781 cgaccttcca tcccctggct ttcgccgccg tccggagccg ccgatccggg ttgacggcga
     9841 cggggttgcc gacggcctcg agcataggca ggtcggagat ggaatcggcg tacgcgtagg
     9901 agcgagtgag gtcgaggcct cggcgcctgg cgaaggaggc cagcatcctg gcgcgggcat
     9961 ctccggctac gggggcgccc gtcagctcac ccgtgtaggc gccgttctcc tgggccagcg
    10021 tggagcagag gacgtcgtcg gcgaggtctt tcatgggctc gaggagaaag tccagcgcgc
    10081 cggagagcag gacgacgcga tggcccgcgg ccttgtggtc tcttagttgc tgcaacgcgt
    10141 cggggaagat gcgatccagg gtaaagccgg cgaagctctc ctgtccgagg tgcctggcgc
    10201 gggatggctt ccagcccgcg tagttcttgt agaaggcacg gttgaagtgg gcgcggctga
    10261 ccttgtccag tccccagtag taaggcacct tggtaaggaa cccggcgagc cagagcggac
    10321 gcaaggtcac aggaagctcc ctcatcctga gccacgcgaa gtaagagacc acgtttgaac
    10381 cgacgatggt gccgtccacg tcgaagatcg ccgcgacctc gccaggcttc tcggccgtca
    10441 ccttcttgcg cttcttgcgg ctcgggcggg tcgtgagcgc gggcaggtgc gctccctgca
    10501 tccacgcccg ccagtcgatg tcggcgatgt cgaacgggaa ctcctcgcgg tcctctggcg
    10561 ggagggctgt gtagaggccc tccgtgcggg agctcgcgaa agtcgaggtc atgttcgcgt
    10621 aggcgccgta gatgtgactg tagtacaggc tcatcttggc gcgcttctcg gcgcgtgcga
    10681 tcctctgccg cgcgtccgaa gccatgtggc cttcaggcat acgcgcggcg agaaggccgg
    10741 ctaccttgag ggcggcaagc tccaccttga gctgtccctc gacccgtctg cggccgggga
    10801 aggaccactc tggtacctgg atcggacgcc cgctggagtc gcgcaagggg ttctgtacaa
    10861 agtagtcgcg cacgatgtcg tagaggtcgc ggtagcggat ggggttgcgt tctcccgagg
    10921 cgacctggaa gatctcgggc tcttcggggc ggcggatccc ggcggccagg atcgcgttca
    10981 cgacgtggtc tacggggacg atgtccacgt agcttttcgg gttgcccggg aactcacgca
    11041 ggatgccctt cgcgaacgcc atgattattg gatcggccat cctggtgccc tggatccaac
    11101 caggataggg ctccctgtgg ctgctctcta tgatggccgg cctcactatg acgagcggcg
    11161 tctcgcccct ttcggcgacc actattcgct ctgcgagcga cttggtgaag gtgtagacat
    11221 cgttccaacc taattcccgc gcccgcctgg ttccccgctc gacgaggcgc tcgcgcatcc
    11281 aggcccgcct cagctggtcg actctctctg caacctcctc atcctcgccc accatcccga
    11341 gctcccgcct cgcctccgtc tcgaagcgcc tcagcagcga ccgctcccgc gaagcctcat
    11401 ccacctcggc cacgaccgct tccaggccga gaacctcctc gcgcgcgtcg agggtcgtcc
    11461 cgttcggcga ggacgggcct ggcggcgcct cgggcgcgtc ctccttggtg attcccgcca
    11521 cgtaggccgt cgagatatga atgaacagcg gcagcttctc ccaggcgcga gcgagctgca
    11581 ggagccccag ggtaccggtg acgttcgagt cgatcgcggc gtcgagcggc gcgtcgaaga
    11641 tgaccgaagc ggcggagtga atcacgacgt ccacctcgcg cgagagctcg gtaaggtcct
    11701 cctcgccaag ccccagcgaa ggcgcgtgta catcgccctc caggacgcgg accttcttcg
    11761 agacatgatc ctcgaagtcg tggccaagct tctcgcgcaa accgcgcaag gctgctgcgc
    11821 cgagcacgtc cctctcgaac ctctctgctg cgctcttacc gcgggatgac ctgatgacga
    11881 ggtagaggcg cccgaggttg gggagggagc gcagtatctt ctcgaccagc gccgtcccga
    11941 ggaagccggt cccccccgta agcagtatct tcttgtcctt gtacgcctcg ccgagcaact
    12001 agctaccctc ctctgcccgc tggcctactt tcctttccgt gccgttcagg agggcccgcg
    12061 cgttctccct gtgcctccac agaacgagcg ccccccctat aatggtgtag agcacgtaag
    12121 ggagtggctg gccggagagc cagaacacca cgggacttat gaccatgacg gctatcgcgc
    12181 cgagcgatgt gtagcggcta aggagcacga ccgcccacca gaacgcgaag aggcagagcc
    12241 ctaccagagg cgcgagccct atggtcgtac ccgcgcctgt cgccacggct ttcccgcccc
    12301 tgaagcgcaa gaatacgggc cagcagtgcc cgatcagcgc cgcgaaagcg acggcggcga
    12361 tgagccattc gttctgcacg gcgagcctgg cgaccaccac aggaacgaga cctttgagca
    12421 tatcgcccgc gagcgtgagt atggcagccc acttccccgc ggcccgcaac acgttagccg
    12481 tacctatatt gccgctgcct accttccgaa cgtctacacc gagagcccgc ccgaccaggt
    12541 acccggtagg cacggagcca agaacatagc cgaacagcac gataagaagc cctaacatgg
    12601 cggagaaagt atagcggaac gagttcccga aggcgtcgct gctacagcca catctcgcgg
    12661 gtgcgggcga cctccagcgc ctgctccatc gcccgcgcct tgttacgggt ctcctcgtac
    12721 tccagcgaag ggacggagtc ggctacgacg ccgccgccgg cctggaagta ggcgacaccg
    12781 tctttgagga gcgctgtgcg gagcgttata caggtgtcga ggcggccgtc gacaccgtag
    12841 taaccagtgg cgcccgcgta aggaccgcgg cgggtactct cgagttcgtc gatgatctcc
    12901 atggccctga ccttcggcgc accggagacg gtcccggcgg ggaaggccgc cgcgagtgcg
    12961 tcgagggccg tcaggtcctc gcgcagatct ccctcgacgg tcgagacgat gtgcatgacg
    13021 tgcgagtagc gctctatctc catgaagctc tctagcccga ccgagccgac ctcactgacg
    13081 cgcccgaggt cgttgcgccc gaggtccacg agcatgacgt gctcggcccg ctctttctca
    13141 tcggccaaga gctcttcggc cagggcggcg tcttcctccg gcgtgccgcc acgtccgcgg
    13201 gttcccgcga cgggccgggt catcacccgc cttccctcga cccgcaccag gggctcgggg
    13261 gatgcgccga cgatagagag gccgccgagc ttgaggtagg tcatataggg ggatgggttt
    13321 accgtgcgca ggccgcggta gaggagaaga gggtcaaggt cccctatgtc cgccgcgaac
    13381 ctctgggaag ggacgatctg gaaagcgtct cccgccctga tgtactcttt cgccgcctct
    13441 acggcctcct cgtagccctc tctggtgaag ttcgaggaga tctcgactcc ggtgccgaac
    13501 gcgggcgtcc tgcgcaccag cggggcggag aggcgctcca cgatcctgcg tacgtcgtcg
    13561 gccgcccgcc tgtatgcggc cgagaacccc tcgccctcca cgtcgcgcag gcccgccgcg
    13621 tcgatcagcg agacgacgag caccttatgc ctgaggtggt cgaagacgat cagggtgtca
    13681 gtaacggcga agcaagcctc ggggacgtcg aggtcgtctc ctggggcgtc ggggaggcgt
    13741 tcgagataac gcacggcgtc gtaggagaag tatcccacgg cgccccccac gaacgccggt
    13801 agattgggaa gcggggctac gctcttctta ccgacgatct cggccaggcc cctgaaagga
    13861 tcccgtgctg gaacctcccg cacaccgtcc gcgtcgacga ccgtgtagag accgtctcta
    13921 taggagagcg tgcgtttggg gtcgaagccg aggaaggagt agcggccgaa cctctcggcc
    13981 gactctgcgc tctccagcag gaataccgtg tcctcctccg cgaacctgag aaccgccgag
    14041 atgggcgtct cgaggtcccc aataaactcg gcgtagagcg gcaccacgtc gtaggaacgg
    14101 gcgagcctgc gggcctcgcc cagcgaaggg acgagacccg ggttagccgt ggcggtcatg
    14161 cagtaccttc gccacgtcct ctatccccac gccccgctcc tcgagcagca ccagcaggtg
    14221 gtagacgagg tcggccgcct cctcgctcag cctctcgtcg gagagtgctg caacaacgac
    14281 ctccaccgcc tcctctccca ctttctgggc caaccgctcc tttccgccct ggatcaggga
    14341 gacggtgtat gagccttcgg gcatctccct gtgacgccgc gctatcgtcc ccgccagccg
    14401 ctcgactatc gtcccgaaag ggatctcccc atcgccagcg gacgcaatac ctatccgttc
    14461 tcctgccagg gtggtgaaga agcagctctt ctggcctgta tggcaggctg gcccgcgcgg
    14521 ttcgaccctg tagaggagtg cgtcgccatc gcagtccagc ttcacctcga cgaccctctg
    14581 cgtgttgccg ctcctcgcgc ccttgcgcca gagctcggag cgcgagcgag agtagtaatg
    14641 ggcctcgcca ctggagagcg tcttctcaaa ggcctcgcgg ttggcgtagg ccagcgtaag
    14701 cacgtcgccc gtcgccgcat cctgggcgac gacggggacg agaccgttct gatcgtactt
    14761 cactctgtcc gtcgctttag tcattcctct atctcatccc tcggtgatac ttggttcaca
    14821 agccggaccg ggatgccggc ttgcatcatg tgctccttga cctcggctat gctgtactcg
    14881 ccgaagtgga agatgctcgc ggcgaggacc gcgcctgctc cggcgttcac ggctgcgatc
    14941 atatgctccg ggctccccgc cccgccggac gcgactatgg ggagggtggt cttctcggcg
    15001 acggcggaga tcagatcgag atcatatcct gtctccgtcc cgtccgcgtc catgctgttg
    15061 agcacgaact ccccggctcc ccgcctctct ccctcgatga gccatccgac tgcgtccatc
    15121 cccgtatcga cccgtccgcc gttcaggtat acctcccagc caggggagag gccgcgccgc
    15181 ttcacgtcga cgctcaacac gacgcactgg ctcccgaagc gttccgcccc ttcagtgatg
    15241 agatccgggt tccccacggc ggcgctgttt atggagactt tgtcggcccc tgcgcgcagc
    15301 atggcgcgca tgtcgccggc cgtccttacg ccgccgccga tggtgtatgg tacgaagacc
    15361 tcctctgcag cagcgcgggc aagcgcggcg gccgtgtctc tctgttcgtg ggaggcggtg
    15421 atgtcgtaga agacgatctc atctgccccc tccctgtcgt agagggccgc gagctccaca
    15481 gggtctccgg cgtccctcag gttggtgaag ttcgtcccct ttaccacgcg cccggcgtcc
    15541 acgtcgaggc atgggatgat gcggactttg aggctcaccg ggagctcatc tcccttcctg
    15601 gtagctcatt acagggtatt gtacggggct acacgtaggg cacaccggcg ttgccacatt
    15661 cttcggccag gcgcttgcgc agggcgttcc tcgccggcag cagcacggag ccctcatctc
    15721 ctgcgcccac tacgatcgag ccgtcccttc ccctccagtg acgg
//
LOCUS       DASTKM010000228         2979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1129063, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000228 DASTKM010000000
VERSION     DASTKM010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129063"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFQ10_09400"
     CDS             complement(<1..563)
                     /locus_tag="VFQ10_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965597.1"
                     /translation="MPSKRPAGDDLRTQKAREEMIQGPSAVDLDVHDFSVRAVGLPLF
                     RRMEDGILSQVLRLVVQAESAAGDVTFTVSDGDTTLARARVRIDDGRTLVHLFVPEVY
                     EPRTFGLRVEARGGEPFEAGVEIRPQRKWSVFLVHHSHLYSIDELARQIRFADELRER
                     YNVLIQTAMQTDVPGATVGLLSSLVDA"
     gene            complement(707..1126)
                     /locus_tag="VFQ10_09405"
     CDS             complement(707..1126)
                     /locus_tag="VFQ10_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9965598.1"
                     /translation="MKITDADHTNWRVKDVERSLEFYRDVLGLEPFGLEEYERGDHPL
                     VSLRVTPEFILHLRPDRTFEAVSTGGYDHLALVVEGTNPDALAEHLTDAGVEIERRSA
                     NVVGARGSGEALYVRDPDGYLIELKIYTDEPGTPRAE"
     gene            complement(1184..1582)
                     /locus_tag="VFQ10_09410"
     CDS             complement(1184..1582)
                     /locus_tag="VFQ10_09410"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9965599.1"
                     /translation="MKAVWLEEFGGPEVLVAADASDPVPGPGQALIEVSFANITFVET
                     MFRASGFGPFETELPMIPGNGIGGVVASVGVETDERLVGKRVVASTGGSGGDVGESAF
                     GLLDRGGRMVRFAHAAIESRATVGKTLLVT"
     gene            complement(1582..2916)
                     /locus_tag="VFQ10_09415"
     CDS             complement(1582..2916)
                     /locus_tag="VFQ10_09415"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9965600.1"
                     /translation="MLGGVPTAFRPLRHRDFRLLWTGLAIALVGSGLWLVALAWQVIE
                     LGGGPTELSLVTALFSVGLLAFVLIGGVAADRLPQRLVMLAADLVRAAVLLVLGLLSL
                     SGDLRIWHLAVSGLLIGAGEAFFIPSYTALLPHLLPADELLAANGLEGTLRPLAQQAA
                     GPALGGIAVAALSPGVAILAGGLAYLVSAGCLLVMRVRPRKVPPANGAPGVASVVSDL
                     REGLGYVRRTSWLWASLLFALVLVLFIIGPLEVLLPFAIRDNLEGGAEDYGLALAAFG
                     VGGAVGAMAISSGRLPRRYLTVMILMWGLGSLPFAVIGVAGALWVMCAALFVVGATYS
                     AAMVIWGTLLQRRVPEGLRGRVSSLDFFVSLALMPVSMAIAGPAGAAFGLAAVFLVAG
                     IVPGFLAVAAIVGPRLDRDELAHPLDHPATDEPTPVKGLPGSTQNESKGSER"
BASE COUNT          572 a         1020 c          929 g          458 t
ORIGIN      
        1 tccgcgtcca ccagggaact aagaagaccg accgtcgccc ctggcacgtc cgtctgcatc
       61 gcggtctgta ttagcacgtt gtagcgctcg cgtagttcgt cggcgaagcg tatctggcgc
      121 gccagttcgt cgatcgaata caggtgcgaa tggtggacga ggaagaccga ccatttgcgc
      181 tggggacgga tctccacccc ggcctcgaaa ggctccccgc ccctggcttc gaccctgagg
      241 ccaaacgtgc ggggctcgta gacctcagga acgaagagat gcaccagtgt gcggccgtcg
      301 tcgatcctga ccctggccct agcgagggta gtatccccgt cggagaccgt gaaggttacg
      361 tctccggccg cggattcagc ctgcaccacc agccgcaaga cctgcgagag aattccgtcc
      421 tccatccggc ggaagagggg taggccaacg gccctaacgg agaagtcgtg gacgtcgagg
      481 tcaacggcgg aagggccctg gatcatctcc tccctggctt tctgcgtccg tagatcgtcg
      541 ccagcggggc gtttgctggg catcatgctc ctctttcgag cgcttgtcag ctggtcagca
      601 attcagcttg tagcctaccg cctttggcct atgggcggtt cgtggtaggt caatacctcc
      661 gcaaatagct tcggtcgtac agcaagcgca cgtctccctg cggacttcat tcggcgcgag
      721 gagtccctgg ctcgtcggtg tagatcttga gctctatcag gtagccgtcc gggtcccgca
      781 cgtacagagc ctcccccgac cctcgggcgc ccacgacgtt cgccgaacgg cgttcgatct
      841 ccacaccagc gtccgtgagg tgttcggcca gcgcatccgg gttcgttcct tcgaccacca
      901 gagcaaggtg atcgtaaccc ccggtggaga cggcctcgaa ggtgcgatcc ggcctcagat
      961 gcaaaatgaa ctcgggcgtt acccgcagcg agacgagggg atgatctccg cgttcatact
     1021 cctccaggcc aaacggctcg agaccaagaa cgtcgcgata gaattcgaga gacctttcga
     1081 cgtcctttac ccgccagttg gtgtgatcag catcggttat cttcattgtt ctcctgaatc
     1141 ctttcatgtg aatctcgcca gtcgggtgca gtatcttcgc tggtcaggta acgagcagcg
     1201 tcttgcccac ggtggcgcga gactcgatgg ctgcgtgagc gaaacggacc attcggccac
     1261 cacggtcaag cagcccgaaa gcggattctc cgacgtcgcc gcccgaaccg ccggtgctcg
     1321 caaccacccg cttgcctacg agtcgttcgt cggtctccac gccgaccgac gcgaccacgc
     1381 cgccgatgcc gttgcccggt atcatcggta actccgtctc gaacggcccg aacccgctcg
     1441 cacggaacat ggtctccacg aaggtgatgt tagcgaacga cacctcgatc aacgcctgcc
     1501 cgggaccggg aacagggtcc gaggcgtcgg ccgcgacgag cacttcaggc ccaccgaact
     1561 cctctaacca caccgctttc atcaccgttc actacctttc gattcgttct gcgtggaccc
     1621 gggcaagccc ttcaccgggg tgggctcatc ggtggccggg tgatctagcg ggtgggcgag
     1681 ctcgtcgcga tctagcctgg ggcccacaat ggccgcgacg gcgagaaacc cgggcacgat
     1741 cccggctacc aggaagacgg ccgctagccc gaaggccgcg cccgccgggc cggcgatcgc
     1801 catggagacc ggcatcaggg ccagggagac gaagaagtcg aggctcgaca cgcggccgcg
     1861 caggccttcg ggcacccggc gctggagcag ggtgccccag atcaccatcg cggcggagta
     1921 ggtcgcgccg acgacgaaca gggcggcgca catcacccac agcgcgcccg ccaccccgat
     1981 aacggcgaac ggcagggaac cgagacccca catgaggatc atgaccgtga gatagcggcg
     2041 cggcagccgt cccgaggaga tagccatggc tccgacggcc ccgccgaccc caaaggcggc
     2101 cagggccaga ccgtagtcct cggccccacc ctcgaggtta tcgcggatcg cgaaaggcag
     2161 cagcacctct aagggcccga tgatgaacag gacgaggacc agcgcgaaca agagcgacgc
     2221 ccacagccag ctcgtccttc gcacgtagcc gaggccctcg cgcaggtcgg agacgaccga
     2281 cgccacgccg ggagcaccgt tcgcaggtgg aactttcctc ggccgcactc tcattaccag
     2341 caggcatccg gcggagacca ggtaggctag gcccccggcg aggatcgcga caccaggcga
     2401 gagcgccgcc accgcgatgc cgccaagcgc gggtcccgcc gcttgttggg ccagcggcct
     2461 gagcgtcccc tccaggccgt tcgccgcgag caattcgtcc gcggggagca ggtgaggcag
     2521 caacgcggtg taggacggga tgaagaacgc ttcgccagcc ccgatcagca gcccgctcac
     2581 ggccaggtgc cagatcctga ggtcgccgga cagcgagagc aacccgagga cgagcaggac
     2641 agcggcgcgg accaggtcgg cggcgagcat gacgagccgc tggggcagcc tgtcggcggc
     2701 gaccccgccg atcagcacga acgcgaggag gccgacgctg aacagggcag tgaccagtga
     2761 aagctccgta gggccgccgc ccagctcaat cacctgccat gccagcgcga ccaaccacag
     2821 tccgcttccg accagcgcga tcgccagtcc cgtccacagc aggcggaagt cgcggtgacg
     2881 caggggacga aatgccgtgg gcacccctcc cagcacggtt actaccgtac catcccgaac
     2941 cgcgtcgccc gtactatccg catgcctacc actctactc
//
LOCUS       DASTKM010000229        18602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1129262, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000229 DASTKM010000000
VERSION     DASTKM010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 18602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18602
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129262"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFQ10_09420"
     CDS             <1..254
                     /locus_tag="VFQ10_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965601.1"
                     /translation="FACVAGAKGEAERAARLWGAAQTLHETKGIPRDIDFLAEADARI
                     SAVRSGMGEEAWEEAQHKGRAMTLDEAVSYAMEKEGAGG"
     gene            795..1604
                     /locus_tag="VFQ10_09425"
     CDS             795..1604
                     /locus_tag="VFQ10_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965602.1"
                     /translation="MAEASREAGRVALRWWRGWPGWIGYVAAAWSLTYGLLGLYWTLG
                     GAGFPFGKNDPGAALSILGSGRADFGAPVIAALGLAGVVAAVAMARTQRGGGVLRAAL
                     LGFAWVAAVTLTLVMPDYRVLVAVAYTPIVLIGTPFGWPPGVSILDVFPWPVVNQFVC
                     IAGGLLWAATAVAYGRRSRGACESCGRDGTRVGWTTPDSAARWGRWATYVAAIIPILY
                     AVTRWAWALGFPLGISEEFLREGQEVGLWRAGAALATLAVGGAILTLGLVQ"
     gene            1701..1907
                     /locus_tag="VFQ10_09430"
     CDS             1701..1907
                     /locus_tag="VFQ10_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965603.1"
                     /translation="MTTAGLMFVRLTLTGRLSAILGEGVLSAENWAALAPKLLWPLWG
                     TALGAATLAYYYRRRGKCEHCDRL"
     gene            complement(2209..3018)
                     /locus_tag="VFQ10_09435"
     CDS             complement(2209..3018)
                     /locus_tag="VFQ10_09435"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9965604.1"
                     /translation="MELGLENKVAVVTGSSKGIGKAIAVELAREGCRVMLAARGEDDL
                     EQAVEDVRRIRGIEDVLAAVADVTKADEVEGLIDETVARFGTVDILVNNAGGTGRRSP
                     FHELSDEEWFEILDLNLISAVRLTRAVLPHMRRQRWGRIINVASESGSQPSALKPHYN
                     AAKAALINLTKSLSKAYGEHGILVNAVSPATTITPAVEELIAEEARRKCLPLHEREAA
                     FVRENKPDIVAGRLGRPQETAWVVAFLASERASFVTGANYRVDGGSVTSIN"
     gene            3338..4078
                     /locus_tag="VFQ10_09440"
     CDS             3338..4078
                     /locus_tag="VFQ10_09440"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9965605.1"
                     /translation="MKTAIVTGATSGIGRAAAVALGKAGWSVMVTGRDESRGRAVVES
                     LDGEGDFLAVDLDEPGSPERLVDRTVERWRRLDLLVNNAGIHARGTVADISGDDYDRI
                     MDVNLRAAVLLAGAAVRAMRSGGGGVIVNVSSEAGIVAVPQQVVYNVSKAGLIMLTKS
                     IAVDHAKDGIRAVTVCPGTTRTPLVEEAIAGAPDPAEHEGVLASSRPANRLGRPEEIA
                     SAIVYAASEDASFLSGTEIVVDGGYTAA"
     gene            4093..5271
                     /locus_tag="VFQ10_09445"
     CDS             4093..5271
                     /locus_tag="VFQ10_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET9965606.1"
                     /translation="MLRRALVWGGAAVLCALVAVGCGGGGAEGGGGEEGRYREEIVIG
                     WTPPDITGVFKTATDFFEQAAQDANKEGFNVRIESRSPETHTAFADQLEIIENFISQN
                     VDVIAVSPSDTEAIKPAIQRANEAGIPVIMVNLLEPQDDIEVASYIGFDNSEAASVSA
                     YSVLDYYGGPGVLGAGEKVDVPQDQYLDLEWWEKTYENADASQIQASGVIIEGIAGTF
                     FSQERLDGFNQVMDEYDGIEILGEPIAADWNREKGVQAAEDFMSRYSPEEMNFMWAAS
                     NEMGLGAMAATQRRGVLDDSGGKTPPAQGKVAIFTNDVTPESTDAIREGKIIAETHHG
                     FPEWGWFGTKFGVQLACGQDVPQTYDIRPRTVWQGNADLFYPNPDLPEINWQQIKNDC
                     "
     gene            5276..6766
                     /locus_tag="VFQ10_09450"
     CDS             5276..6766
                     /locus_tag="VFQ10_09450"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET9965607.1"
                     /translation="MALLKVEGVAKSYPGVRALAGVDLSVDPGEVHALLGENGAGKST
                     LMKVIAGYVTPDAGHMELDGLPVPFGSPETARSHGIGIVYQELSLVPQLSIAENVLLG
                     TWPTNGRLIDWRTLHTEAKGHLERVGLRLDPDLPVQGLGMAERQLVEIAKALSSEVRV
                     LLLDEPTSALSDREAERLFELIGGLKNSEVAIIYVSHRLSEVLDIADRITVLRDGEKV
                     ETLPAEGVAEEHLAQMMVGHELSDGLAEHASRRSQVGAEIVLRARGLARPPRLRPVNL
                     ELREGEVVTAFGLVGAGRTRLARTLFGLEPAADGSLELFGEERRISSPADAIAAGIGF
                     VSEDRTAGLVPRMSVANNITLASLEDVARGPVLDFAREKRVAEQYVGDLAIRTSSLDQ
                     LVETLSGGNQQKVVLARWICSRARVLILDDPTRGIDVGAKEEVFGLVRRLADDGIAIL
                     YMTSEIREARELGDRLLVMSGGRIVKEFAAGASEEEIMTAAGGAHD"
     gene            6759..7766
                     /locus_tag="VFQ10_09455"
     CDS             6759..7766
                     /locus_tag="VFQ10_09455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9965608.1"
                     /translation="MTKTAKGTLARVVTVAGGRGGALIILFLLVVAFAIVTPDFLTVN
                     NLINILRQYSVLMILAVGQTLVIVSRGIDLSVASTAALSGSVMGVAYAYWAWPEPASL
                     LVGLAAGVAVGAFNGFVITRLDVPDFIATLGTFVAVRGIALLVTDGLPVPDFERAVEG
                     RTLPGTISTLGVDSVLGAPLIAVVALVCALVGAFILARTRLGRSAYAIGGNTEAARMS
                     GIKVERSKMWIYVFSGLLAAVGGFMLTGRQASANALMGEGLELQSIAAVVIGGTNLFG
                     GEGRMSGTVIGVLIIGVLSNGLSIAGVAEFWQRVVNGLIIVAVVALDQWRRRAARAAS
                     S"
     gene            7819..8409
                     /locus_tag="VFQ10_09460"
     CDS             7819..8409
                     /locus_tag="VFQ10_09460"
                     /inference="COORDINATES: protein motif:HMM:NF018288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HET9965609.1"
                     /translation="MSTQAPDPFTTLLDLTSGSLNPERKVVERRLSDMAGMYLERNAD
                     GTADTVVYSVHEIPVPTTNSNLMSSTTVIQPGMVGREYYMTKGHFHKVRDRAEIYIGL
                     SGEGRLVMAKEDGHYTVEAMCRGTINYVPGGWAHRSVNVGNEPLVFFAVYPGDAGYDY
                     GTIEEEGFPVLVIAGEDEPEVVPNPRYKGAAASEAG"
     gene            8406..9944
                     /locus_tag="VFQ10_09465"
     CDS             8406..9944
                     /locus_tag="VFQ10_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET9965610.1"
                     /translation="MTELLVGVDLGTTGTKTAVYDPNGKPLAEANAEVPLRWQGPGRV
                     DQDPEDFYRSTVSTISRCLERGGVAAEQVVALGITGQMAGVLGLGAGWKPSTPYDSWL
                     DLRCSEDVDWLERELGDVLVEKTGCPPMIDHAPKMRWWHRERPEVFAAAEKWIMPSGY
                     VAGKMAGLEPGDAFIDSTYLHFTGLAEARGGAWSGELAEAIGIPTDKLPRIVEPSTVI
                     GRVSEEAAADCGLMEGTPIVAGLGDTAAGALGAGLVRSGQLLDVAGTAAVLAASTDEF
                     RPDVRSRTLIAMRGAVPGQWLPLSYLSGGSLLGWLQEALAPVGEEVYEPDFDELTAEA
                     REVRAGTEGLLFVPHLDGRLLPSDPAMRGAWVGLHRHHKRPHLVRAVLEGVAYEYAYY
                     LQVLLTLYPGLSFDETRVIGGGAHSDTWNGIKASVLGVPYVRLDREEFSCWGAALVAG
                     HAVGLFDDLAGAAERSTLTRDRFEPDPEDHAAYRPVAQLYRDLLAALAEPSHRLVRLA
                     AEMP"
     gene            9941..10969
                     /locus_tag="VFQ10_09470"
     CDS             9941..10969
                     /locus_tag="VFQ10_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9965611.1"
                     /translation="MSQLRIAMVGAGRAGLVHARNITEHTPDAVVVAVVDGEPDRAKS
                     LAKIVGTTSYHTTLDEALEEVSFDAVVISTPTFTHRDLAVQAAGAGKHVFCEKPMALT
                     VEECDDMIHAAESVEVALQIGFMRRFQEEFVEARRRIAAGDIGDPMVVKSLTRGPGLP
                     PAWAWDLEKSNGMLAEVNSHDFDCVRWLVGSEIARVYAETVNKKGASRGVEIANFYDN
                     AVVTLRFASGALGTIDGTCPADYGYDARVEIVGSEGLLIVGDVRGQPIVEARNREVGA
                     ITPLHRTWPERFEQGYRDEMRSFVDVALSGDRPAVTGEDGRHAVSAVRAANRSWMEER
                     PVALENGT"
     gene            10969..12003
                     /locus_tag="VFQ10_09475"
     CDS             10969..12003
                     /locus_tag="VFQ10_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9965612.1"
                     /translation="MEALVYHGPNDLRVEDRAPPEPGFGEAVLRVGACGICGTDLRIA
                     TGGHRAYPPGTVRIPGHEVAGTILAVGDGVDLKEGQPAFIAPNVGCGRCAQCRAGRVN
                     LCVEPEAVGISMDGGLADQLLLPANLVAQGNVVPVPETADLRAVALAEPLACVLRGSE
                     ACHIRAGDLVVIVGAGPVGLMHLLVARLADPSAVIVSQRSEERRRLALEYGADEAVGP
                     DDLAATVMDVSGGRGADVVIVAAPAAEAQRQALEVAAPAARINFFGGLPRGKSVVELD
                     TNLIHYKELVVTGATANTTEDCQRALGLVLGGSIDTAALISRSYRLAQADEALAAAGS
                     GEALKVVIEP"
     gene            12077..12928
                     /locus_tag="VFQ10_09480"
     CDS             12077..12928
                     /locus_tag="VFQ10_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET9965613.1"
                     /translation="MLFGAHAFIWTGEWTPEGAEKAIGGAAEARLDFVEIPLLRPESM
                     EVAVTRALLDRYGIGCTCSLGLPKVAHLPSAPEKAESFLESAVDVAAGLEAPVLCGVL
                     YAHLGTLTGRPPEEEELGDVAGVLKNVARYAADREVSLGVEAVNRYETYLINLAEQAD
                     AMLERVGEPNVFVHLDTYHMNIEEKGFYEPIVATGERMRYIHLSESDRGTPGTGNVHW
                     DEVFRGLRAIDYDGYLVMESFAAINEDLAGATALWRDVVGDPEALIRDGLSFLRGKAT
                     EYEVLRG"
     gene            12947..13924
                     /locus_tag="VFQ10_09485"
     CDS             12947..13924
                     /locus_tag="VFQ10_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9965614.1"
                     /translation="MITKEPGVWPSVVVTARSFGSGDADPAGLLQKAGLMVAHADPAH
                     DHAVLEEILSAAVAWIAGTSPIDDLHLAAAPKLRIVARYGTGYDSVDLAACAHRGVIV
                     TNTPGANAEAVADHTVGLMLAALRHLIAGDRASREGVRPPLRGRELGALTVGIIGFGS
                     IGRAVARRLIGGFGSRVLAYDPFVTPERAREAGIEPVDDLSGLARAADVLTLHMPGGG
                     GAVLDAAFLSRMRPGAVLINTARGDLLDEQAVASALAEGRLAAAAVDVLASEPATASP
                     LLDAPNAIITPHVAAQTTQAIDRMGMMAAEEVVRVLVGEAPRHPVAVPT"
     gene            13937..14605
                     /locus_tag="VFQ10_09490"
     CDS             13937..14605
                     /locus_tag="VFQ10_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET9965615.1"
                     /translation="MDKQAILKRIAELGLLAVLRAPDPAGARRTADALVEAGVLGIEI
                     TYSTPDAASVIAGVKQSYGDEVLVGAGTLVTHAQVSEAAEAGASYLVSPGLDDEVVAS
                     MRATGLPAMAGVLTPTEVMRGVRLGVDVMKLFPGSLGGPSYLRSLRGPFPDVPFMPTG
                     GVSADNVGDWLAAGAIAVGAGSELASAGDISSGDFAGIREKGRRFLTAIREERADAAP
                     RASR"
     gene            14602..15555
                     /locus_tag="VFQ10_09495"
     CDS             14602..15555
                     /locus_tag="VFQ10_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965616.1"
                     /translation="MSAGSVETRPVPTMEFIGVTTGSSSIMKVFPRWAQVLDLGEAKL
                     KGRDLPLGSGPGLYRQAVEQIRSDPLSMGALVTTHKINLLAAAHDLFDELDPYARLCE
                     EVSCISKRDGRVIGYAKDPITAGRSLRALLDPGHFRRTGGEVLCLGAGGAGTAISVYL
                     MSGQTPADPPGRIVMVDKSQESLDALRRVHDRLGRSSTEVEYVENANSAENDELLAGL
                     PPGSLIVNATGMGKDVPGSPVTVSARFPESGVVWELNYRGELDFLRQARRQEHTRHLR
                     VEDGWSYFLHGWSEVIAEVFHVELTPERFELLSAEAEAIRS"
     gene            15647..16615
                     /locus_tag="VFQ10_09500"
     CDS             15647..16615
                     /locus_tag="VFQ10_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965617.1"
                     /translation="MQRLGDKKVRTVLGDIDPRDLGKTDVHEHLLMHSPLLRGEELDD
                     LEKSTQEAVELRQTGIDALVELTTIGLGRVPRGVADIAAHSGLHIVLATGIHREAHYP
                     PEHWVHRTATEELAELFLRDITEGCDGTDYSGPREQPTRIRAGVIKVGARYWSISPLE
                     RRVFEAAGEAHRGTDAPVACHLELGTAAWEVLEALEAAGIAPERIMLAHVDRNPDPGL
                     HAELAAAGAYLGYDGMARFKYWPDSVLLDCLLRVASQGGADRLLLGGDVARRTAFRSY
                     GGLPGMAYLPQRFVPRLAEMGGEELVRRVLINNPARFLAFAPPQGG"
     gene            complement(16618..17439)
                     /locus_tag="VFQ10_09505"
     CDS             complement(16618..17439)
                     /locus_tag="VFQ10_09505"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9965618.1"
                     /translation="MYTTRRIDHDSPVPFYYQLKQILVEEIETVRYEPGDKLPSEHDL
                     CQAFKVSRTVVRQALNELEMEGLLRRRKGRGTFVMPKKVSESLFQNLTGLYEDVAARG
                     GMLRSEVRQLERVPAPIQVAAELGLQEGDSVIVLDRLRFVNGVPWVVVATYLPYELCP
                     QLLQEDMEAQSLYAVLEQKYGIRIAYGRRSVEATTATATIARDLAIEERDPILLLRST
                     SYDEDDRPVEYFVAHHRGDLSRFEVNLVRRRNEGSGVPGVVPTMVTDAASERDRA"
BASE COUNT         3354 a         5710 c         6272 g         3266 t
ORIGIN      
        1 gatttgcatg cgtggctggg gcaaagggag aagcggagcg ggcggcgcgg ctgtggggag
       61 cagcgcagac gttacacgaa acaaagggca ttccaagaga catcgacttc ctcgccgagg
      121 ctgatgctcg catttccgct gtgcgctcag gcatgggaga ggaagcatgg gaggaggcgc
      181 agcacaaggg ccgagcgatg accctagatg aggctgtatc ctacgccatg gagaaggagg
      241 gggcaggagg ctaatctctt tgctagcact tgcgaacata aacgaatttc ccgcatgcga
      301 acttcgcaga aagcgtgctc cactagtgac gagtgacagc ggtattgacc ccgtgtcagc
      361 tgtacagagt ggaggtcgtt gacaccactt ctgacaccac tgagggcgca acacctagaa
      421 acgacgggca accgcgagca aagaaattag cttatctaag ccgattctgc aacgtacggc
      481 aacacgcaga aacaggtgtc ggcggattgt ggctctagag aaggtcgcgg gttcgattcc
      541 cgccggtcac cctattccgc cgcaccaagg gaggtagggc gcagctggag gaagcgggct
      601 gctcccaggg tagaattggt agtggtcggt gcttttagag taggacggca atcttgaagc
      661 agcattgaag gctgaaggcg acgcgctcca agaagagatc gtctccttgc ggcgtcatct
      721 ccaccgatat ccggaggtga gcggactaac acgccacctc agagacagta cataacatcc
      781 aaaaaggagc cgagatggcg gaggcgtcgc gcgaggcagg tagagtggcg cttcgctggt
      841 ggcgagggtg gccgggctgg atcggttatg tagcggccgc ctggtcgcta acgtacggcc
      901 tgctcggcct ttactggacc ctgggtggcg cgggcttccc cttcggtaag aacgatcccg
      961 gcgccgcact ctcgatcctc ggaagcgggc gcgccgactt cggtgcgccg gtgatcgctg
     1021 cgctggggct ggcgggcgtg gtggcggcgg tggcgatggc ccgaacgcag cggggaggcg
     1081 gagttcttcg cgcggcgctg ctcggcttcg cctgggtcgc tgcggtgacg ctaaccctgg
     1141 tgatgcccga ctatcgcgtg ctcgtggcgg tcgcctacac gcccattgtt ctgatcggca
     1201 cccccttcgg ctggccgccc ggagtgagca tcctcgacgt cttcccctgg cctgtggtga
     1261 accagttcgt ctgcatagcc ggcgggcttc tgtgggctgc gacggccgtg gcttacgggc
     1321 gccggagcag gggcgcgtgc gagagttgcg gccgggacgg cacccgcgtc ggttggacca
     1381 cgcctgactc ggcggcgcgg tggggcaggt gggccaccta tgtcgcggcg atcattccca
     1441 tcctgtacgc cgttacgcgc tgggcgtggg ctctgggttt cccactgggg atctccgagg
     1501 agttccttcg cgaaggtcag gaggtcggcc tgtggagggc cggagctgcg ctggcgactc
     1561 tggcggtcgg cggcgcgatt ctcacgctcg ggctggttca gtaatggggc gaggtctttc
     1621 cacgttggat cccgttcctg ggcggcaatc gagttcccat tcctttagct gtaatccccg
     1681 cttcgctggt agctgtcctg gtgaccacag caggcctgat gttcgtccgc ctaacgctca
     1741 ccggcaggct cagcgcgatc ctcggcgagg gagtattgag cgcggagaac tgggcggcgc
     1801 tcgcccccaa gctgctgtgg ccgctgtggg gaaccgcgct cggcgcggcg acactcgcct
     1861 actactaccg caggcgaggc aagtgcgagc actgcgaccg actgtaacgg gccggatcct
     1921 ggaaaggtac tggagtatga tcaaacaatc taaccggacc tcgaaagcgt cgagacccac
     1981 gtagaggcac agcgggttct ggagcaagac ccgagcgacc cgcactacct cgacggcgac
     2041 ggcgacggcg tgtgaggagc tgcgctgaat gaagtcctaa ggtagagacg actcaactaa
     2101 ggggatggtc gaactgctct gcgctaccgt tttgggtgtg gcgaataggg tgccgcaggc
     2161 tacgtcgtac gcccccggcc tctcctcgtg ttggccagcg atatcgccct aatttatgga
     2221 ggttacagag ccgccatcga ctcgatagtt ggctcccgta acgaacgacg ctctctccga
     2281 agccagaaac gctaccaccc acgccgtttc ttgtggtctg cccaagcggc cggcgacgat
     2341 gtccggtttg ttctccctaa cgaaggcggc ctccctctca tgaagcggga ggcactttcg
     2401 cctcgcttct tcggctatga gttcttcgac ggctggtgtt atcgtggtcg ccggagagac
     2461 cgcattcacc aagattccat gctccccata tgccttcgat aggctcttcg tgaggttgat
     2521 gagtgcggct ttggccgcgt tgtaatgtgg tttcagcgcg ctcggctggc tccccgattc
     2581 cgacgcgacg ttgattatgc gaccccacct ttgtcttcgc atgtggggca ggaccgcgcg
     2641 cgttaagcga accgccgaga tcaggttgag atcgaggatc tcgaaccact cctcgtcaga
     2701 gagctcgtga aagggcgagc gcctgccggt tcctccggcg ttgttaacga ggatgtctac
     2761 cgtcccgaag cgcgcgacgg tctcgtcgat caacccctcc acttcgtccg ctttggttac
     2821 gtccgcgacg gcggctagca catcctcgat gcccctaatc cggcgaacat cctcgaccgc
     2881 ttgttctaga tcatcctctc cgcgcgcagc aagcatcacc cgacagcctt cccgggccaa
     2941 ctccacggcg atcgccttcc ctatcccctt gctggatccc gtaacgacgg ctactttgtt
     3001 ttccaaaccc agttccacga tctgtcctct cagctcttgt ttttcgacga ccagaacatg
     3061 accataatgc taatggaaac gatactctac ctagtctcgc gcgtttccag agttgtcacc
     3121 ttctcgggcc cctctctctg cggccgcaga gaaagaactg gcgatccact tgacccggca
     3181 gccactacgc agtactcttg catgtaacta caggaggaca ggcttagggg atgactttta
     3241 cgctggcggc ttcggtgagg gctccgcgcg taatttcctt aggggcgttg gtctctctac
     3301 tgcccggggg aaccggttca tgcgtggggt tggggtagtg aagaccgcta tcgtgacggg
     3361 ggcgacgagc ggtatagggc gcgccgctgc cgtcgcgctc ggcaaggccg gctggtccgt
     3421 catggtcacc ggccgcgatg aatcgcgcgg tcgcgcggtt gtggagtcgc tcgacggtga
     3481 aggagatttc ctcgccgtgg accttgatga gccgggatcg cccgagcgac tcgtcgatcg
     3541 gaccgtggag cgctggagga ggttggatct gctggtcaac aacgccggga ttcacgcccg
     3601 tggtacggta gcggatatat ccggggacga ctacgatcgg attatggacg tcaacctccg
     3661 cgccgcggtg ctcttggcgg gcgcagcggt ccgggctatg agatccggcg ggggtggggt
     3721 catcgtcaac gtttcgagcg aggctggcat cgtggcggtg ccgcagcagg tggtctacaa
     3781 cgtctccaag gccggactca tcatgctgac gaagtctata gctgtggacc acgcgaagga
     3841 cggtatccgt gcggtcacgg tgtgccccgg gacgacgcgg acgccgctcg tcgaagaggc
     3901 catcgctgga gcgccggacc cggccgagca cgagggggtg cttgcgtcca gcagaccagc
     3961 caaccggctc ggcaggccgg aggagatcgc ctccgcgatc gtctatgcgg cgagcgagga
     4021 tgcttcgttc ctgagcggga cggagatagt tgtcgacggc ggttacacgg ccgcctgagg
     4081 taaggaggag tcatgctcag acgtgcgttg gtgtgggggg gtgcggcggt actttgcgcg
     4141 ctcgtcgcgg ttggctgcgg cggtggtggt gcggagggcg gtggcggcga agaagggcgc
     4201 taccgggaag agatcgtcat cggctggacg ccgcccgaca tcaccggcgt gttcaagacg
     4261 gcgaccgact tcttcgaaca ggctgcgcaa gacgcgaaca aagagggctt caacgtgagg
     4321 atcgagtccc ggtcgccgga gacccacaca gcgttcgccg accaactcga gattatcgag
     4381 aacttcatct cgcagaatgt ggacgtgata gcggtctcac cctcagacac cgaggccata
     4441 aagcccgcca tacagcgggc taacgaagcc gggatacccg tgatcatggt caacctgctt
     4501 gagccgcagg acgacatcga ggtcgcgagc tacatcggct tcgacaactc cgaagccgcc
     4561 tccgtttcgg cgtattcggt gttggactac tacgggggcc caggcgttct cggcgctgga
     4621 gagaaggtgg acgtgccgca ggatcagtac ctcgacctcg agtggtggga gaagacctac
     4681 gagaatgccg acgcgagcca gatccaggcg agcggcgtga ttatcgaggg tatcgccgga
     4741 acgttcttct cccaagagcg gctcgacggc ttcaaccaag tcatggacga gtacgacggc
     4801 atcgagatac tcggcgagcc cattgccgcc gactggaacc gtgagaaggg cgtccaggca
     4861 gcggaggact ttatgtcccg ctacagcccc gaggagatga actttatgtg ggccgcatcc
     4921 aacgagatgg gcctgggcgc tatggccgcc acccagcgca ggggcgtgct cgacgactcg
     4981 ggtggcaaaa cgcctcctgc gcaaggcaag gtcgcgatct tcaccaacga cgtgacgccc
     5041 gagtccaccg atgcgatcag agagggcaag atcatcgccg agacccacca cggcttcccg
     5101 gagtggggct ggttcgggac caagttcggg gtgcagctcg cctgcgggca ggacgtcccc
     5161 cagacctacg acatccgccc gaggaccgtg tggcagggca acgccgacct gttctacccg
     5221 aacccggatc taccggagat aaactggcag cagatcaaga acgactgcta gcgccttggc
     5281 cttgctcaag gtagagggtg tagcgaaaag ctacccgggt gtgagggcgc tcgccggggt
     5341 ggacctcagc gtcgaccccg gcgaagtaca cgccctgctt ggcgagaatg gggccggcaa
     5401 gtccaccctg atgaaggtga tcgccggcta cgtcacaccg gacgcgggcc acatggagct
     5461 cgacggccta ccggtcccgt tcggctcgcc agagactgcc cgctcgcacg gcataggcat
     5521 cgtctatcaa gagctcagcc tcgtgccgca actctcgatc gccgagaatg tgctgctcgg
     5581 cacgtggccg acgaatggcc ggctgatcga ctggcggact ttgcacaccg aggccaaagg
     5641 acacctcgag cgcgtcggcc tcagactgga cccggacctg cccgtgcagg ggctggggat
     5701 ggcagagcgg cagctcgttg agatcgccaa agcgctctcg agcgaggtgc gagtgctgct
     5761 cctcgacgaa cccacctcgg cgctctccga ccgggaggcc gaacgcctct tcgagcttat
     5821 cggcggtttg aagaactccg aggtggccat catctacgtc tcccaccgtc tgagcgaggt
     5881 cctcgacata gctgatcgga tcaccgtgct acgggacggc gagaaggtgg agaccctacc
     5941 ggcggagggg gtggccgaag agcacctcgc ccagatgatg gtcggtcacg aactttcaga
     6001 tggcctcgca gagcacgcgt ctcgacggtc ccaggtagga gcggagatcg tcctccgcgc
     6061 tcgtggcctg gcgaggccac cacgtctcag accggtaaac ctggagctgc gggagggcga
     6121 ggtcgtcacg gcctttggcc tcgtcggggc gggcaggacg cgtctcgcac gcacgctttt
     6181 cggcctggaa ccggcggccg atggctccct ggagctcttc ggggaagaga ggcgtataag
     6241 ttccccggcc gacgccatcg ccgccgggat cggcttcgtc agtgaggacc gcaccgccgg
     6301 actcgtccca cgcatgagcg tggcgaacaa cataaccctg gcctccctcg aagacgtcgc
     6361 ccgcggcccg gtcctcgact tcgcccggga gaagcgggta gcggagcagt acgtcgggga
     6421 cttagccatc cgtacgtcat cgctggatca gctcgtcgag acgctctccg gcggtaacca
     6481 gcagaaggtc gtgctcgcga ggtggatctg ctcgcgtgcg agggtgctca ttttggacga
     6541 cccaacccgc ggcatcgacg tcggggccaa ggaggaggtc ttcggccttg tgcgccggtt
     6601 ggctgacgac gggatcgcga tactttacat gacctcggag atccgagagg cgcgggagct
     6661 gggggacagg ctgctcgtga tgtccggcgg gcgcatagtc aaagagttcg cggccggcgc
     6721 ttcagaagaa gagataatga cggcggcggg aggagcgcat gactaagacg gcgaagggga
     6781 cgctggcgag agtggtgacc gtcgctggcg gtaggggagg agctctcatc atcctctttc
     6841 tgctcgtcgt cgccttcgcg atagtgacgc cggacttcct cacggtcaac aacctcatca
     6901 acatactgcg ccagtactcg gtgttgatga tcctggccgt cggacagacc ctcgtgatcg
     6961 tctcccgagg catagatctc tccgtcgcct ctaccgccgc gctctctggc agtgtgatgg
     7021 gtgtggccta cgcgtactgg gcatggccgg agccagcatc gctgctcgtc gggctggcgg
     7081 cgggggtcgc cgtgggcgcc ttcaacgggt tcgtcatcac gaggctggac gtgcccgact
     7141 tcatcgccac cctcggaacc ttcgtcgcgg tgcgcggaat cgcccttctc gtcaccgatg
     7201 gcctacccgt gcccgacttc gagcgggcgg tcgagggccg caccctcccc ggcacgatct
     7261 ccacgctcgg cgtggactct gtgttggggg cgccgctcat cgccgtcgtt gcgctggtct
     7321 gcgccctggt cggagccttc atactggcgc gcaccagact ggggcgctct gcgtacgcga
     7381 tcgggggcaa taccgaagcg gcgcggatgt cgggtataaa ggttgagcgg tcgaagatgt
     7441 ggatctacgt gttctcgggg ctgctggccg cggtgggtgg ctttatgcta acgggcaggc
     7501 aagcgagcgc gaacgccctc atgggcgagg gcctggagct gcagtctatc gctgccgtgg
     7561 tcatcggcgg cacaaacctt ttcggcggcg agggcaggat gagcggcacc gtaatcgggg
     7621 tgctcatcat aggcgtcctc tccaacggcc tgtcgatcgc cggcgtcgcc gagttctggc
     7681 agcgggtggt gaacggtctg atcatcgtcg ccgtggtggc gctcgaccag tggcggcggc
     7741 gggcggcgcg ggcggcgagc tcgtgagctc cgctacgcgg ttcacagaga acgctaccaa
     7801 cgggaaagga ggactgcgat gagcacgcaa gcgccagatc cgttcacgac gctgctcgac
     7861 ctgaccagcg gctcgctgaa cccggagcgc aaggtggtcg agcgccggct ctcggatatg
     7921 gccggcatgt acctcgagag gaatgccgac ggtacggcag acacggtcgt ctacagcgtc
     7981 cacgagatcc cggtgccgac aaccaactcg aacctcatgt cttcgaccac ggtgatacag
     8041 ccgggcatgg tcgggcggga gtactacatg accaagggac actttcataa ggtccgcgat
     8101 cgggccgaga tctacatcgg cctctctggc gagggacgcc tcgtgatggc aaaggaagac
     8161 ggccattaca ccgtggaggc gatgtgcagg gggacgatca actacgtccc cggcggctgg
     8221 gcgcaccgca gcgtcaacgt ggggaacgag ccgctggtct tcttcgcagt ctacccaggc
     8281 gacgccgggt atgactacgg caccatagag gaggagggct tcccggtgct cgtcatcgcc
     8341 ggtgaagacg aaccggaggt agtcccaaac cctcgctaca agggcgcggc ggcgagcgag
     8401 gcaggatgac agagctcctc gtaggcgtcg acctcgggac cacgggcacc aagacggccg
     8461 tgtacgatcc caacggtaag ccgctagccg aggccaacgc cgaggtcccg ttgcgctggc
     8521 aaggtccggg cagggtagac caggaccccg aagacttcta ccggtcaacg gtgagcacca
     8581 tctctcgttg cctggagcgg ggtggcgttg ccgccgagca agtggtggcg ctcggtatca
     8641 cggggcagat ggccggcgtc ctaggtctcg gcgccggctg gaagccatcg acgccgtacg
     8701 attcgtggtt ggacctgcgt tgctcggagg acgtcgactg gctagaacgc gagctcggcg
     8761 acgtcctcgt cgagaagacc ggctgcccgc cgatgatcga ccacgcaccg aagatgcgct
     8821 ggtggcaccg cgagcgcccg gaggtcttcg ccgccgccga gaagtggatc atgcctagcg
     8881 ggtacgttgc gggcaagatg gccggcctgg agcccggcga cgcctttatc gactcgacgt
     8941 acctccactt tacgggactc gccgaagcgc gcggaggcgc gtggtcgggc gagctcgccg
     9001 aggcgatagg catcccgacg gacaagctgc caaggatcgt cgaaccctcg acggtgatcg
     9061 ggcgcgtcag cgaggaggcg gccgccgact gcgggctcat ggaaggtacc ccgatcgtgg
     9121 ccggactcgg cgacaccgcc gccggggcct tgggagccgg tctggttcgg tcggggcagc
     9181 tgcttgacgt ggccggaact gccgccgtgc tcgccgcctc caccgacgag tttcggcccg
     9241 atgttcgctc ccggacgctc atcgccatgc gtggcgccgt cccgggacag tggctcccac
     9301 tctcctatct ctccggcggg tcgctcctcg gctggttgca ggaagcgctt gctcccgtgg
     9361 gcgaggaggt ctacgagccc gacttcgacg agctgaccgc cgaggcaaga gaggtccggg
     9421 cgggcaccga gggtctcttg ttcgtgccgc acctcgacgg gcgtttgctc ccgagcgacc
     9481 ccgccatgag gggcgcgtgg gtaggtctgc accgccacca caaacgcccg cacctcgtgc
     9541 gtgcggttct ggagggagtt gcctacgagt acgcatacta cctgcaagtg ctgctgacgc
     9601 tctatcccgg gttaagcttc gacgagacgc gcgtcatcgg cggtggcgcg cacagtgaca
     9661 cctggaacgg catcaaggcc tctgttctcg gcgtaccgta cgtccggctc gaccgcgagg
     9721 agttctcctg ctggggtgcg gcgctcgttg ccgggcatgc tgtgggcctc ttcgacgact
     9781 tggccggtgc cgccgaaaga agcaccctga cgagggaccg cttcgagccc gatcccgaag
     9841 accacgccgc ctaccgaccc gtggcgcagc tctaccggga cctgctcgcg gccctcgccg
     9901 agccgtcaca ccggctcgta cggctagccg cggagatgcc gtgagccagc tgaggatcgc
     9961 catggtaggc gcgggacgag cgggcttggt gcatgctcgg aacatcaccg aacacacccc
    10021 cgacgccgtg gtggtggcgg tcgtggacgg cgagccggat cgggcgaaga gcctcgcaaa
    10081 gattgtcggc acgaccagct accacacgac gctcgatgag gccttggagg aggtgagttt
    10141 tgacgcggtg gtgatctcga ctcccacctt tacccaccgc gaccttgcgg tgcaggccgc
    10201 aggggccggc aagcacgttt tctgcgagaa gccgatggcg ctcaccgtcg aggagtgcga
    10261 cgacatgatc cacgccgccg agagtgtcga ggtcgcgctc cagataggct tcatgcgccg
    10321 cttccaggag gagttcgtcg aggcccgacg acggatcgct gcgggcgata tcggcgatcc
    10381 catggtcgtc aagtcgctca cccggggccc cggcctgcct ccggcttggg cgtgggatct
    10441 ggagaagtca aacgggatgc tcgcggaggt aaacagccac gacttcgact gcgttcgctg
    10501 gctcgtcggc tcggagatcg cgagggtcta cgccgagacg gtgaacaaaa agggggcgtc
    10561 gcgcggcgtc gagatcgcaa acttctacga caacgccgtg gtcacgctcc gcttcgcgag
    10621 cggcgccctc ggcacgatag acggcacctg tccggcggac tacggctacg acgcgcgggt
    10681 ggagatcgtc ggctccgagg ggcttttgat cgtcggcgac gtgcgcggac agcccatcgt
    10741 cgaggcgcgt aaccgtgagg tgggagctat cacgccgctg caccgcacct ggcccgagcg
    10801 ctttgagcag ggctaccgag acgagatgcg ctctttcgta gacgtcgccc taagcggtga
    10861 tcgcccggcc gtcacagggg aagacggacg ccacgccgtg agcgccgtcc gggccgcgaa
    10921 ccgctcgtgg atggaggagc gaccggtcgc gctggagaac ggaacctgat ggaagcgctc
    10981 gtctaccacg gtcccaacga cctccgggtg gaggatcgtg cgccccccga gcccggattc
    11041 ggcgaggcgg tcctgcgcgt cggtgcgtgc ggcatatgcg gcaccgacct gcggatcgcg
    11101 acgggcggcc accgcgcgta cccacccggc acggtgagga tcccgggaca cgaggttgcg
    11161 ggcaccatcc tggcggtcgg tgatggggtc gatctcaagg agggccagcc cgcattcatc
    11221 gccccgaacg tcggctgcgg gcggtgcgcg caatgccgcg ccggtcgagt gaacctctgc
    11281 gtcgagcccg aggcagtcgg gatcagtatg gacggcggtt tggccgacca gctgttgctc
    11341 ccggcaaacc tggtcgccca gggcaacgtc gtgccggtac ccgagacggc cgacctccgc
    11401 gccgttgccc tggccgagcc gctggcctgc gtgctgcgcg ggtctgaggc ttgccacatc
    11461 agggccgggg accttgtggt gatcgtgggc gcaggaccgg tagggctcat gcacctgctc
    11521 gtcgcaaggc tcgccgaccc gagcgccgtg atcgtgagcc agagaagcga ggagcgtcgt
    11581 cgcctggcgc tggaatacgg ggccgacgag gccgtcggcc ccgacgatct cgcggcgaca
    11641 gttatggatg tgtcgggggg caggggtgcc gacgtggtga tcgtcgcggc cccggcggcc
    11701 gaggcgcaac gccaggcact cgaggttgcg gccccggccg cccgcatcaa cttcttcgga
    11761 ggcctcccgc gcggtaagtc cgtggtcgag ttggacacca acttgatcca ctacaaggag
    11821 ctcgtcgtca cgggcgcgac ggcgaacacc accgaggact gtcagcgggc gctcgggctg
    11881 gttctgggtg gctctataga tacggccgct ctcatcagca ggagctaccg cctcgcgcag
    11941 gccgatgaag ccttggcggc tgccggctcg ggtgaggcgt tgaaggttgt catcgagcca
    12001 taagggcccc taggggttgt cggggcgacg gtcggaagtc tggtcgaccc tggagggcac
    12061 gaaggaggtc acagtaatgc tgttcggagc ccacgccttt atctggacgg gcgagtggac
    12121 gcccgaaggg gcggagaaag ctatcggggg ggctgcggag gccaggctgg acttcgtgga
    12181 gataccccta ctgcgccccg agagcatgga agtcgccgtc acccgcgcgt tgctggatcg
    12241 gtacggcatc ggatgcacct gctcgctcgg gctgcccaag gtggcgcatc tgccctcggc
    12301 accggaaaag gcggagtcgt ttttggaaag cgcggtggac gtcgctgccg ggctggaggc
    12361 tcctgtgctc tgcggtgtgc tctacgcgca cctcggaacg ctcacgggca gaccacccga
    12421 ggaggaggaa ctcggggacg tcgcgggggt gctaaagaac gtcgcgcggt acgccgccga
    12481 cagggaagtt tcgctgggcg tggaggcagt gaaccgctac gagacgtacc tcataaacct
    12541 cgccgagcaa gccgatgcca tgctggagcg ggttggggag cccaacgtct tcgttcacct
    12601 ggacacttac cacatgaaca tcgaggagaa gggcttctac gagccgatag tggctacggg
    12661 ggagaggatg cgctacatcc acctctcgga gtccgaccgc ggcacgcccg gcacgggcaa
    12721 cgtccactgg gacgaggtgt tccgcggcct gcgagccatc gactacgacg ggtacctggt
    12781 gatggagtcc ttcgcggcga tcaacgagga tctcgcgggc gcgaccgccc tgtggcggga
    12841 cgtggtcggc gacccggaag ccttgatccg cgacgggctc agtttcctgc gcggcaaggc
    12901 caccgagtat gaggtgttgc gtggttaggc gcgtcggttg tctcctatga ttaccaaaga
    12961 gcccggggta tggccgtccg tagtcgtaac cgccaggtcc ttcggttccg gtgacgccga
    13021 ccccgccggt ctgctgcaga aggcggggct tatggtggcg cacgccgatc cggctcacga
    13081 ccacgccgtc ctcgaggaga tcttgtccgc ggccgtggcg tggatagccg ggacgtcccc
    13141 gatcgatgat cttcacctgg cagcggctcc gaagctaagg atcgtggcgc gctacggcac
    13201 cggctacgac tccgtggacc tcgctgcttg tgcccacaga ggggtgatag tgaccaacac
    13261 ccctggcgcg aacgcggagg cggtggccga ccataccgtc ggtttgatgc tggcggccct
    13321 ccgtcacctg atagcggggg acagagcctc gcgtgagggt gtccgtcccc ccctgcgagg
    13381 ccgcgaactg ggcgccctga cggtggggat cataggtttc gggagcatcg gtcgcgcggt
    13441 cgcccgtcgg ttgatcgggg gcttcggctc ccgggtgttg gcctacgacc cgttcgtcac
    13501 gccggaacgc gcacgagagg ccgggatcga accggtcgat gacctgtccg gtctagctcg
    13561 ggcggcggac gtgttaacgt tgcatatgcc gggcggcggg ggcgctgtgc tcgacgccgc
    13621 cttcctaagc cgtatgcgtc ccggcgccgt cttgatcaac acggccaggg gtgacctcct
    13681 ggacgagcag gccgtcgcca gcgctctggc agaaggacgg ctcgcggcgg ccgcggtgga
    13741 tgtcttagcc tccgaaccgg ccaccgccag ccctttgctg gacgcgccca atgcgatcat
    13801 tactccgcac gtagcagcgc agaccacgca ggcaattgat cgtatgggaa tgatggctgc
    13861 cgaagaggtt gtgcgcgttc tggtgggaga agcgcctcga cacccggtgg ccgtaccgac
    13921 ttaggaggtt tcctacgtgg acaagcaagc aatcctgaaa cgcatcgccg aactcgggct
    13981 gctggccgtt ttgcgtgccc cggaccccgc cggcgcccgg cggacggccg acgccctcgt
    14041 cgaggccggc gttttgggta tcgagatcac ctactcgact ccagacgcgg cgtccgtgat
    14101 cgcaggcgtc aaacaaagct atggcgacga ggtgctcgtg ggtgcgggca cgttggttac
    14161 ccacgcccag gtatccgagg ccgcggaggc cggcgcctca tacctggtca gccccggtct
    14221 cgacgacgag gtggtagcgt cgatgagagc caccggcctg cccgccatgg ccggtgtcct
    14281 cacgccgacg gaggtcatga ggggcgtgcg actgggcgtg gacgtgatga agctgttccc
    14341 gggttctctg ggaggcccct cttatctgcg ctctctgcgg ggcccgttcc ccgatgttcc
    14401 ttttatgccg accggcggag tctccgcaga caacgtgggg gactggcttg cggctggagc
    14461 catcgcggtg ggcgcgggga gcgaacttgc ctcggccggc gacatcagct ccggcgactt
    14521 cgcgggtatc cgcgagaagg ggcggcgctt tctgacggcg atccgcgagg agagggccga
    14581 tgcggcaccg agggcgtctc ggtgagcgca ggatcggtcg agacccgtcc cgtcccgacc
    14641 atggagttca tcggggtcac cacggggagc tcctctataa tgaaagtctt ccccaggtgg
    14701 gctcaggtgc tcgacttggg cgaagccaag ctcaaggggc gcgacctgcc cttaggatct
    14761 ggacccggcc tctaccggca ggcggtcgag cagatccgga gcgatccgct ctcgatgggg
    14821 gctctcgtga ccacacacaa gatcaacctt ctcgccgctg cccacgatct cttcgatgag
    14881 ctcgaccctt acgcccggct ctgcgaggag gtctcttgca tctcaaaacg tgacgggcgg
    14941 gttatcgggt acgccaaaga tccgatcacc gccggccgat ctctgcgagc gttgctggac
    15001 cctggtcact tcagacgtac gggtggggag gtgctgtgtc taggggccgg cggcgcgggc
    15061 accgccatct cggtctacct gatgagcgga cagacccctg ccgacccccc gggccggatc
    15121 gtgatggtgg acaagtcaca agagagcctc gacgccctaa ggagggtgca cgaccgcttg
    15181 ggtcggtctt cgaccgaggt cgaatacgtc gagaacgcca attcggccga gaacgacgag
    15241 ctgctagctg gcttgccgcc agggtcgttg atcgtgaacg ccaccgggat gggcaaggat
    15301 gtacccggct ctccggtaac cgtcagtgct cgcttcccgg agagcggcgt cgtctgggag
    15361 ctcaattacc ggggggagct ggatttcctc cgccaggctc gtcgccagga gcatacccgc
    15421 catcttaggg tcgaggacgg ctggtcctac ttcctccacg gctggagcga ggtaatagcc
    15481 gaggtcttcc acgtcgagct tacccccgag cgctttgagt tgctgagtgc cgaagccgag
    15541 gctatccgca gttgagaaag atgagctagg agtcgttcga gggagtcaaa cacacccagt
    15601 tcgacgggtg agacggtgag gggaatcgcg cctcaagggg gcagctgtgc agcggttggg
    15661 tgacaagaag gtccgcaccg tcttgggaga catagacccg cgggacctcg gaaagaccga
    15721 cgtacacgag cacctgctca tgcactcacc gctgctgcgc ggtgaagagt tggatgatct
    15781 ggagaagtcg acccaagagg ccgtcgagtt gcgccaaacc gggatagatg ccctcgtcga
    15841 gctgaccacc atcgggctcg gcagggtccc acgaggcgtg gccgacatcg ctgcccacag
    15901 cgggctacac atagtgctcg ctaccggcat acaccgagag gcgcattacc caccggagca
    15961 ttgggtgcac aggaccgcga cagaagaact cgccgagctg ttcttacgag acatcacgga
    16021 agggtgcgat ggcaccgact actccggtcc tcgggagcag ccgacgcgaa tccgcgcggg
    16081 cgtcataaag gtcggcgcca ggtactggag catctcgcca ctcgagcgtc gtgttttcga
    16141 ggctgccggc gaggcgcacc gaggcacgga cgcccccgtt gcctgtcacc tagaactcgg
    16201 tactgccgcc tgggaggtcc tggaagccct ggaggccgcg ggtattgccc cagagcgcat
    16261 catgctggcg cacgtcgatc ggaatcccga tcccggcctc cacgccgaac tggcggcagc
    16321 cggtgcctac ctagggtacg acggcatggc gaggttcaag tactggccgg actccgtgtt
    16381 gttggattgt ctgctgcgcg tcgcgtcaca aggaggcgcc gatcgcctgc tgctcggcgg
    16441 cgatgtcgcc cggcgtacgg cattccgctc gtatggtggc ttgccaggaa tggcgtacct
    16501 cccgcagcgt ttcgttccgc gccttgccga gatgggggga gaggagctgg tgcgccgagt
    16561 actaattaac aaccccgcgc gatttctcgc tttcgcaccc ccgcaaggcg gctaattcta
    16621 agcgcggtcg cgctcggatg cggcatccgt gaccatcgtg ggcaccacgc cagggacgcc
    16681 ggaaccctca ttgcgtcgcc gcaccaggtt cacctcgaag cggctcaagt cgccacgatg
    16741 gtgcgcgacg aagtattcga cgggacgatc gtcctcatcg taagaagtgc tgcgcagcag
    16801 taggatagga tcacgctctt cgatcgccaa gtcccgggcg atggtagcgg tggccgtcgt
    16861 ggcttcgacc gagcgcctgc cgtaggcgat gcgaatgccg tatttctgct cgagcaccgc
    16921 gtacaaagac tgagcttcca tgtcctcttg cagcagttgg ggacacaact cgtaaggcag
    16981 gtatgtggcg acgacaaccc acggcacccc attgacaaac ctcagccggt ccaagactat
    17041 gacgctatca ccctcctgca accccaactc cgctgccact tggatcggag ccggtacgcg
    17101 ttcgagctgt ctcacctcgc tccttagcat acccccgcgg gcggcaacgt cctcgtagag
    17161 accggtaaga ttctggaata ggctctcgga gaccttcttg ggcatcacaa aggtcccacg
    17221 acccttccgc cgccttagca acccttccat ctccagttcg ttaagcgctt ggcgcaccac
    17281 ggtgcgcgac actttgaagg cctggcacaa gtcgtgctcg ctgggcaatt tgtcacccgg
    17341 ctcgtagcga accgtttcga tctcttccac gagtatctgc tttagctggt agtagaaggg
    17401 cacaggtgag tcgtggtcta tgcgacgtgt cgtatacatg taatcctata ataacagcgc
    17461 cacgaaccca cctccaacgg tggtcgcaac aatgcagact cgcaaagaca cgaaaggttg
    17521 gcgactacgg tgtcaaagcc ccttaatatg aatgtccgat ggtctcgagt agttgtcatc
    17581 atggacgttg tcgccccgac ttccggcaag ctcttctctt agaccaacga taaactcggt
    17641 ggtcatcctc cctacctacg gcacggcccg cgtcctcctg gccggcgacg ccgaggccag
    17701 ggaggaggag tatatggcag gaggccccta cacgagacct taaacggtta tcagggtccg
    17761 gctcagcaag aatccacgga gagtagcgag caacgtaatg cttgtgaggg gtcacaggga
    17821 gaaagaaagg aataataaga ggccgccgtc catggaccgg ggtgtttact gcaaccgggc
    17881 tagaacagaa ctggacggag caggaaagca gaggcgccga aagccccaaa aaccctttag
    17941 ataagcggga tttcagacag agtaggaccc agcaggaaac ccgcgaccta acttacaagg
    18001 agggggtcgc tggttcaaat ccaacttcgc ccccttaggg gcgtcactag ggtaggggtg
    18061 gaacgtagca gaccgtgggt ggccccggtg aggtcctcgg tgcgacaaga cccatcggga
    18121 ccggacgaac ggctgatgcc atttgacacc acttctgaca ccactcagag cgctacacgc
    18181 agaaacggct agcaaccacc agcaaagaaa tcggcttatc taagctaatt ctgcaacgta
    18241 ccgcaacgcc tggaaacagg ggtcggcgga ttgtggctcc gaaggtcgcg ggtcggtgcc
    18301 attggacctg ccgtcagact gtagctgtcg ctcctcttga taccaatctg ataccaccgg
    18361 gagcgcaata cggtgcaaca cgcagcaacc cggagcagag aaagtcgctt agatatgggg
    18421 gatttgcaag ctcgtgcaaa cccctgcaac gtatgaatta tcactcgtaa tgagcaggtc
    18481 agcggttcga gtccgctcgt cggctctcct gattcgacct agataagccg aatacgcgga
    18541 atgaagaagc ccttggcgac ctaccacagg cttctctaca tcaccggtac atcaccgaga
    18601 ca
//
LOCUS       DASTKM010000230         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_777087, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000230 DASTKM010000000
VERSION     DASTKM010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777087"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFQ10_09510"
     CDS             <1..556
                     /locus_tag="VFQ10_09510"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET9965619.1"
                     /translation="GIDYTKEYFERCLMREEPFEVLPADHWSDDLWYQDQENRAFVPN
                     PGYEVMYEGEAEGKLLGGNLKTLCLLFGTQYMPNLEGSILLLEDDEEVNAVHFDRELQ
                     SLIHQPGFEGVRGIMFGKFQRASNMDQETLMAIVESKRELDGMPVVAGASFGHTTPQF
                     TFPIGGYGSLHATEGTAHLSIDVH"
     gene            complement(772..2403)
                     /locus_tag="VFQ10_09515"
     CDS             complement(772..2403)
                     /locus_tag="VFQ10_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET9965620.1"
                     /translation="MPQLEIILGLLLAVAALATLATRLGVPYPILLVLGGSALGFVPG
                     LPPVELDPELVFLLFLPPLLYVSALFTSWRDFRANVRPITLLAVGLVLMTTFVVASVA
                     HTVVGLPWGAAFVLGAIVSPTDAIAATSVAQRLGVPRRIVTILEGESLVNDATGIVAY
                     RVAVAAVVTGAFSVWEAGLQFVVGAAGGAAAGFAVGWLVVWARRHLSEDPSVQNTISL
                     LTPFVAYLAAEELPHSLWGLLHDLFGVPGDLYFSGVLAVVTTGLYLGRKGPYIISSGT
                     RLQGYATWELITFLLNGLIFILIGLQLRSVVEGLNEYTAGQLVSYALLTSLTVILVRM
                     VWVFPATYVPRWASRRIRESDPSPSWRNVSIIAWTGMRGVISLAAALALPLQTASGVL
                     FPDRDLIIFLTFCVILATLVVQGLSLPVLIRALGLEDDRIGDKEETHGRVSIAEAALE
                     RLDELVDEDWVREDTAERMRGLYSYRRNRFASRFEGDPEGVEERSAAYQHLLVELLGA
                     QRLRLITMRDEGSIGDEVMHRIERDLDLEESRLEL"
     gene            complement(2427..2726)
                     /locus_tag="VFQ10_09520"
     CDS             complement(2427..2726)
                     /locus_tag="VFQ10_09520"
                     /inference="COORDINATES: protein motif:HMM:NF023070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965621.1"
                     /translation="MIVLVNLKEDVAPQEYEHWLQERYVPAILDLASVDEWRGYRVGG
                     LPESGGEPPYEYVVSVGINDLEQLGKDMESEQVQRLLGELGRYAEVTQLMTERFV"
     gene            complement(2857..3138)
                     /locus_tag="VFQ10_09525"
     CDS             complement(2857..3138)
                     /locus_tag="VFQ10_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET9965622.1"
                     /translation="MRRFIHMFQRKRCSHTSQIKDVEPSAQGCEDCLRTGDTWVHLRE
                     CLICGHVGCCDQSPNRHATKHFHATEHPIIRSLEPGEDWRWCYVDEVLV"
     gene            complement(3171..>3631)
                     /locus_tag="VFQ10_09530"
     CDS             complement(3171..>3631)
                     /locus_tag="VFQ10_09530"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HET9965623.1"
                     /translation="DQFAGLYEEAAAGDLDGWRDDARSCLALVIVLDQFPRNMFRGDG
                     RTHAADAAALESSRYAVEHALDRELPAFQRMFLYMPFMHSENREDQRRSVELFGRLAD
                     DPGAPDVTSYAAGHRDIVERFGRFPHRNGILGRETTSEEAEFLKTEGSSF"
BASE COUNT          700 a         1160 c         1130 g          641 t
ORIGIN      
        1 cggcatcgat tacacgaagg agtacttcga gcggtgcctc atgcgcgagg agcccttcga
       61 agtccttccc gctgatcact ggagcgatga cctctggtac caggaccagg agaaccgtgc
      121 cttcgtaccc aaccccggct acgaggtcat gtacgaaggc gaggcggagg gcaagctgct
      181 cggggggaac ctgaaaaccc tgtgcctgct attcggcacc cagtatatgc cgaacctgga
      241 ggggtccatc cttctcctcg aggatgatga ggaggtcaat gccgtccact tcgaccggga
      301 gttgcaatct ctcattcacc aacctggttt cgagggcgtg cggggcataa tgttcgggaa
      361 gttccagagg gcttcgaata tggaccagga aaccctgatg gccatcgtcg agagcaagcg
      421 tgagctggac ggtatgcctg tcgtcgccgg tgcgagtttc ggccacacca cgccgcaatt
      481 cacgttcccg atcggtggtt acggcagtct gcacgccaca gagggaaccg cccacctctc
      541 catagacgtc cactgaaagc agttgcagga agttctcttc ctgcaactgc gctggtcggc
      601 acggcggctg cgccttgtca gcacgctcag gcttacgcct tcgcttctca gcatggcggg
      661 ttttgcggta ggagatgact ctcaaagagt acctactcct gtggcattca tctcaccacc
      721 cttgacattg ctgagtggct gacaagcgaa agccgaaggc tggagcgtgc ttcacaactc
      781 caggcgggac tcttccaggt cgaggtcgcg ctcgatgcgg tgcatgacct cgtctccgat
      841 gctaccctcg tcgcgcatcg tgatgagcct caggcgctgg gcccccagca gctcgaccaa
      901 gaggtgctgg taggcggcag agcgttcttc gacgccctca ggatcgccct cgaagcggga
      961 ggcaaagcgg ttgcggcggt agctgtagag gccgcgcatg cgctcggccg tgtcctcgcg
     1021 cacccagtcc tcgtcgacaa gctcgtccag gcgctccaag gctgcctctg cgatactgac
     1081 gcgaccgtga gtctcttcct tatccccgat gcggtcgtcc tcgaggccga gggcccggat
     1141 caggaccggc agggagaggc cctggaccac gagcgtcgcg aggatcacgc aaaacgtcag
     1201 aaagatgatg aggtcgcggt cgggaaagag cacccctgag gccgtctgaa gcggcagcgc
     1261 gagtgcggcg gccagcgaga tcacaccccg catccctgtc cacgctatga tcgagacgtt
     1321 gcgccaggac ggagagggat cactttccct gatcctgcga ctggcccacc tcgggacgta
     1381 cgtcgcgggg aagacccaga ccatgcgcac gaggatgacg gtcaagctgg tgagcagcgc
     1441 gtaggagacg agttggccag cggtgtactc gttgagcccc tcgaccacgc tcctgagctg
     1501 aagcccgatc aggatgaaga taagaccgtt cagcagaaac gttatcagct cccacgttgc
     1561 gtacccctgc aggcgcgtcc ccgatgagat tatgtaagga cctttgcgcc caagatacag
     1621 ccctgtcgtc accaccgcga gaaccccgga gaagtagaga tctcccggca cgccgaacag
     1681 atcgtgcagc aatccccaga ggctgtgcgg cagctcctcc gccgccaggt aggcgacgaa
     1741 gggcgtcagc agcgagatgg tgttctgtac ggaggggtct tctgagaggt ggcgtcgcgc
     1801 ccagacgacg agccaaccga ccgcgaagcc agcggcggct ccgcccgccg cgccgacgac
     1861 gaactgcaat cctgcctccc agacagagaa ggcgccggtg accacagcgg cgaccgcgac
     1921 gcggtaagcg actatacccg tggcgtcgtt gacgaggctc tcgccttcca ggatcgtaac
     1981 gatgcggcgg ggcacgccga ggcgctgggc gaccgaggtg gcggcgatgg cgtcggtggg
     2041 ggagacgatg gccccgagta cgaaagccgc gccccagggc agcccgacga cggtgtgggc
     2101 gactgaggcg accacgaagg tcgtcatcag caccagtcct acggcgagca gcgtgatcgg
     2161 cctgacattg gccctgaagt cgcgccaact agtaaagagc gccgagacgt acagcaaggg
     2221 cggcaaaaac aacaggaaca cgagctccgg gtcgagctcc accggcggca gtcccggaac
     2281 gaaccccagc gccgatccgc cgagaaccag caggatcggg taaggtaccc cgagccgcgt
     2341 ggcgagcgtg gcaagcgccg ccaccgcaag gagaagaccg agaattatct caagctgggg
     2401 caacgctcac gtccgcactg tcaggctcag acaaaacgct ccgtcatgag ctgtgtgacc
     2461 tccgcgtacc ggccgagctc acccaggagg cgttgtacct gttcgctctc catatccttg
     2521 ccgagctgtt ccaggtcgtt gatgcctacg gaaacgacgt actcgtaggg tggctcgccg
     2581 cccgattccg gaaggccgcc caccctgtag ccacgccact cgtctaccga ggcgaggtca
     2641 agtatcgcgg ggacgtagcg ttcctggagc cagtgctcgt attcttgtgg agccacgtct
     2701 tccttcaggt tgaccagcac tatcatcgtc ggcaccgctg ctcctctctc agccattctg
     2761 tctcagttcc tcggtgaggt ttgtggaccc cgatcgagcc gtcgccgggc aggaggaact
     2821 cttcagaccc ttacaagtgg aaccagttat cccaacttag accagcacct cgtcgacgta
     2881 gcaccatcgc cagtcttccc ctggctccag ggacctgatt atggggtgct cggtcgcgtg
     2941 gaagtgcttt gtggcgtggc gattggggct ctgatcgcaa cagcccacgt gcccacagat
     3001 aaggcactcc ctgagatgaa cccaggtatc tcccgtcctc aaacaatctt cgcagccctg
     3061 cgccgatggc tccacgtcct tgatctggct cgtgtggctg caccgctttc gctggaacat
     3121 gtggatgaac ctcctcatag atgttctcct caggcgggcc gccctcatgc tcagaacgac
     3181 gagccctccg tcttcaagaa ctcggcctcc tcggacgtgg tctccctgcc caggatccca
     3241 ttcctgtgcg ggaaccgccc gaaccgctcg actatgtccc tgtgccctgc ggcgtaggag
     3301 gttacgtcgg gggcccctgg atcgtccgca agccgcccga acagctcgac ggagcggcgt
     3361 tggtcctccc tgttctcgct gtgcatgaac ggcatgtaca agaacatcct ctggaaggca
     3421 gggagctccc tgtccagcgc gtgctcgacg gcgtacctgg aggactcgag cgccgctgcg
     3481 tcggcggcgt gggtgcgtcc gtcgccacgg aacatgttgc gggggaactg gtcgagcacg
     3541 attacgagcg ctaggcagct ccttgcatcg tcgcgccagc cgtcgaggtc gccggctgcg
     3601 gcctcctcgt agagaccggc gaactggtcc g
//
LOCUS       DASTKM010000231         9476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1306474, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000231 DASTKM010000000
VERSION     DASTKM010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306474"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFQ10_09535"
     CDS             complement(<1..224)
                     /locus_tag="VFQ10_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965624.1"
                     /translation="MASRNITIDTITKIEGNAGLKVTIEDEAVKDLRFIVSDYRRFFT
                     TAVKGRRMIAVPSFLSRICGTCSVAHLFASL"
     gene            complement(217..750)
                     /locus_tag="VFQ10_09540"
     CDS             complement(217..750)
                     /locus_tag="VFQ10_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965625.1"
                     /translation="MTTERSPHQASSSDQHKRLVVGWFSFTCCEDSTILLTELLNDHL
                     DEWKEVIEFTHMKALKTNNSLAGLDVAFIEGAISSESQANEVRRIRENAKYVVAIGAC
                     ACTGQPSTSRNQFVSDQLSERIRWYLSHFDYGKEVKSLGEVIQVDDMVRGCPMKVPSY
                     FQTLDKYLDLFDIPQHG"
     gene            complement(779..1855)
                     /locus_tag="VFQ10_09545"
     CDS             complement(779..1855)
                     /locus_tag="VFQ10_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9965626.1"
                     /translation="MESPHERALRDIEEAFGDRLKREGPALPGVPDAGDTLASVSPMS
                     VEEVKLLAEVAGRYSVPLVALGAGMASKPEPEGGGILIHFDLMRGLRLPEREESWVEA
                     EPGASWLQLDDLLRARGWGLAVYPTSAPLTTVGGWLAMDGLGVGSYEYGWLRENVPWA
                     SVVLPGGEVVEVAGEEVRSFVGPGAAGIIVGAKLRTRRAEADVPFGDAFGTADELTGA
                     VANIAEMGLPLWHLAFLNPGMALARNLGENYLLFGAYPAERAGEIDGDLRNVVASSRG
                     RALSAAEAHRAWGERFFPVTPSALPPRFSVLSREVVSLAELPEKLSEVEDRPGNAAVQ
                     GTVVRSGEVLLLSLEDHEEGSARL"
     gene            complement(1996..2739)
                     /locus_tag="VFQ10_09550"
     CDS             complement(1996..2739)
                     /locus_tag="VFQ10_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET9965627.1"
                     /translation="MEPVGATSVFLFALLTAVATGLGAVPFAFAKHPTRRWLGASNAV
                     AAGLMLAASFGLLYEGMNYGPLRAMAGALLGLAFIVVARRSLRQEEHPAVFASMSGLD
                     ARKALLIVGVMTLHSFTEGVGIGVSFGGGQALGLFISLALAVHNIPEGLAISLVLVPR
                     GVGWVRAAFWSIFSSLPQPLMAVPAFFFVEVFRPLLPYGLGFAAGAMIWMVFSELVPD
                     ANEEASSNLVAVVITVSVVAMVAFQVLIR"
     gene            complement(2785..3276)
                     /locus_tag="VFQ10_09555"
     CDS             complement(2785..3276)
                     /locus_tag="VFQ10_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965628.1"
                     /translation="MAEGEFVRRLGEAGYGPNNARGELLDLLACAGFLVHEGLVLTQR
                     AHEEFLRTSGALRDIRAVTKRGEDGWQRAEVIRSRRVSYPVEGRLNRAICKALVELNA
                     RAVIVLSADFARGGLRSIPEVKDAVRDAWLTQRGLERQVDAAAQGEVPPTWPLLVYPQ
                     AKI"
     gene            complement(3475..4146)
                     /locus_tag="VFQ10_09560"
     CDS             complement(3475..4146)
                     /locus_tag="VFQ10_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9965629.1"
                     /translation="MDDDKRVRELPPAVRSLIEARTGDAVYVVDPDYRIVHWDSQMES
                     LTGALSQEAIGQPCYEAVMGEREDGGPFCAHGCSVMHLAQGHRPVSAFEMRIKTRSGD
                     RRWVSVSNLTLETEEGTYLVHLIRDTQGTHDALEMAHGLIQLSSKKEAPAPRRRDVPA
                     LTSRQLEVLRLLSEGKSVKEIGKELYISEATVRNHVRSLLQALGAHSQLEVLAKAREM
                     GILGG"
     gene            complement(4272..5402)
                     /locus_tag="VFQ10_09565"
     CDS             complement(4272..5402)
                     /locus_tag="VFQ10_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9965630.1"
                     /translation="MNDIPEVALRTIEDIFGNSFVRHADGEADAGQLFASVFPENAAQ
                     VESLTKLAARYRIPLIARGAGTAQNSGRAPRALAVRFDAMREIRITESGEDRVEVEPG
                     VTWMVLEERLRERGIGPTVYPTSAPRSTVGGWLAENGLGVGSFEYGWLLQNVLSLEVV
                     LAGGERDLIEGETLRHFVGSRGSMGFLVRARLATRPAAGDVPEGVVFRDVEDLAAAVL
                     DLYRGGAPLWHLGFLNAAMTRASGLEEGHVLFGAYPEERASWIEPALDRASESHRGRV
                     VPRVEARHIWEQRFFPAFPLGPTPMPGRAFIRGARLGPTLAELERELVGVAIQGTVAR
                     MGEVALLAFDPAKGSAGLVDLSATTDIELVRLAGRSWMPERR"
     gene            complement(5434..6036)
                     /locus_tag="VFQ10_09570"
     CDS             complement(5434..6036)
                     /locus_tag="VFQ10_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965631.1"
                     /translation="MMDIQLEVLLAVGYAIFLLGVAFGLDLLARHSHARSERYRTAGF
                     TYHPSHDAWVCPEDQLLVRSEVDHERRLVRYRGRPQICNYCPAKAECTDSDVGREVVR
                     AMDPWPHSEAGRFHWGISLAVTVIAGLILAAEAIRNHAPVELAVLGAVLTVVVLVAWH
                     LHAAFRATPVNFPAPRVAPSSKIPMNPVQTGYRKGKRVER"
     gene            complement(6038..8344)
                     /locus_tag="VFQ10_09575"
     CDS             complement(6038..8344)
                     /locus_tag="VFQ10_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HET9965632.1"
                     /translation="MVGGSERRELLISKGWVQAVALVVLFGFFVLGLLAYRAYSGQPP
                     IPERVVDPGGEVVFTGEDVRAGQGVFLQNGLMQYGSIFGHGAYLGPDFTADYLRRAAL
                     FVRESYGGESSDRAAVQTVSDFKENRYDPETRTLEFTAPQAAAFEELEEHYGRYFGEP
                     TTKYGLRPEAIKDPEDIRQLTAYFAWTAWAASALRPGTDYTYTNNWPPEPLVENEPTA
                     NVVVWSVLSLIALLGGIGLLFAAFGRWNFLGWRGREQATLSFRTPGDVALTPAQRACA
                     WFFLVMAALFLVQTLVGAASQHYRADLASFFGIDLAQWLPYNLMRTWHVQLAIFWVAT
                     SFLAAGIFLAPMIAGREPRGQRLLAFGLLGALAIVVFGSLIGEWAGIFGLTGGLWAWF
                     GNQGFEYLDLGRFWQILLSIGLFFWVVMLFRAMRGRLRREHMGNMPWLFFFAALAIPA
                     FYAVGLLARPESHFTTADFWRFWVVHLWVEDFLELFTTVMVAYIFVLLGVVREKVALT
                     VIYLDILLYSAGGVIGTMHHLYFSGTPAEHMALGAFFSAAEVIPLTFLTVEAWSFLQL
                     GARQESRSPTPFPHRWAVMFLVAVGFWNFLGAGIFGFLINLPIISYYQIGTALTANHG
                     HAAMMGVYGMLAAGLALFCLRYLIPEERWSERAARMSFWSLNIGLAWMCFATLFPLGI
                     LQLYQSVSVGYYEARTLEFLTNETNALLEWIRLPGDVVFIVGGVLPLLYLCYLGVRHT
                     VPRVVVEEPEDILFTEITEPEATGSRER"
     gene            complement(8460..8888)
                     /locus_tag="VFQ10_09580"
     CDS             complement(8460..8888)
                     /locus_tag="VFQ10_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9965633.1"
                     /translation="MKLELSSEGRYTLRALVYLAWVGERVTADRISAEAHIPRRLLAR
                     ILAKLSHAGLVETEKGRGGGSRLARPANKITLREAVDTVEGPFSIVRCIMEDRACGEG
                     APCALHDAWVEGQKAILEHLGTQTIKEFISRTASDKFPRA"
BASE COUNT         1883 a         3157 c         2782 g         1654 t
ORIGIN      
        1 agagaggcga agagatgggc gaccgaacac gtgccgcaga tcctggacag aaacgatgga
       61 accgcgatca tgcgcctgcc ctttaccgcg gtcgtgaaaa agcgcctgta gtccgagacg
      121 atgaaccgca gatccttgac tgcttcgtct tcgattgtga ccttcaggcc ggcgttgccc
      181 tcgatcttcg tgatggtgtc tattgttatg tttctactag ccatgctggg gaatatcgaa
      241 taggtccaga tacttatcta acgtctgaaa gtaagaaggc accttcatcg ggcatccacg
      301 caccatgtca tcgacctgta tcacttcgcc caggctcttg acctctttgc cgtaatcgaa
      361 atgactcaga taccaccgga ttctctcgct tagctggtca ctaacaaatt gattgcggct
      421 cgtcgagggc tgtcccgtac aggcgcacgc gcctattgcc accacgtact ttgcattctc
      481 tcgtatccgt cgaacctcat tcgcctgcga ctcggatgag atcgcccctt cgatgaatgc
      541 cacatcgagt ccggcaagcg aattgttcgt ctttagcgcc ttcatgtgcg taaattcgat
      601 cacctctttc cattcatcta ggtggtcgtt gagtaactcc gtaagaagga tcgtgctgtc
      661 ttcacaacag gtgaacgaaa accagcctac aacgagtcgc ttatgctgat cactgctgct
      721 cgcttgatgt ggtgatcgct cagtagtcat gtggtccgat atcacttctg gcctctcact
      781 ataaccgtgc cgaaccttcc tcgtgatcct ccaggctcag gagcagcacc tctccagacc
      841 tgacgacggt gccctggact gcggcgttac ctggacggtc ctccacctcg ctaagcttct
      901 cagggagttc cgccaacgag accacctcgc gagatagaac cgaaaaccgt gggggaaggg
      961 cggaaggcgt gaccgggaag aatctctccc cccacgcacg gtgggcttcc gccgcggaga
     1021 gggcacgacc ccgagaggac gcgacgacgt ttcggaggtc accatcgatt tctcctgccc
     1081 gttctgccgg ataggccccg aagagcaagt agttctcacc aaggtttctg gcgagcgcca
     1141 tgccggggtt caagaaagcc aggtgccaga gcggcagacc catctcggct atgttcgcca
     1201 cagcgcctgt gagttcatcc gccgttccga aagcgtcacc gaagggtacg tccgcctccg
     1261 cccgccgggt gcgcagcttc gcgccaacga tgatccccgc cgccccaggt cccacgaagg
     1321 atcgcacctc ctcccccgcc acctcgacga cctctccgcc cggcaggacc acgctcgccc
     1381 acgggacgtt ctctcgtaac cagccgtact cgtaggaacc cacgccgagg ccatccatgg
     1441 ccagccagcc ccctacggtg gtcaggggcg cgctcgtagg atagaccgcg agaccccatc
     1501 ccctggcgcg caggaggtcg tccagttgca gccacgatgc ccccggctcg gcctccaccc
     1561 aggactcttc gcgctcaggt agacgcaggc cgcgcatcag gtcgaagtgg atcaggatac
     1621 ccccgccctc gggttccggc ttcgatgcca tcccggcacc gagcgcgacc aacggcacgg
     1681 agtaacgccc ggccacctcc gcgagaagct tgacctcctc gacactcatc ggcgacacgg
     1741 aagctagagt gtctccagca tccggtactc ccggcagagc cggtccttca cgcttcaacc
     1801 ggtctccgaa ggcctcctca atgtccctca gggctcgctc gtggggagat tccatcgcct
     1861 acgtcctttt gttcggttac accgggaact tactatcgca cagtcagctc ttcctgagca
     1921 atcttgatgg ttcctgccat ccatgttgac catcttccgc cgcgttccgt ctggtcgagc
     1981 gtatcgtgga gatgcctacc ggataaggac ttgaaaagcc accatggcga cgactgagac
     2041 cgtgatcacc accgctacga ggttgctcga agcttcctcg ttggcgtcgg gtacgagctc
     2101 ggagaagacc atccagatca tggcccccgc cgcgaagccc aacccgtagg gcagcagcgg
     2161 ccggaagacc tcgacgaaga agaacgccgg caccgccatc aggggctggg gcaggctgga
     2221 gaagatgctc cagaatgcgg cgcgaaccca gcccacccca cgcggcacga gtaccaggct
     2281 gatggcgagc ccctcgggta tattatggac ggccagagcc aggcttatga acaggccgag
     2341 ggcctggccc ccgccgaaag atacccctat ccccacccct tcggtgaacg agtgtagcgt
     2401 catgacgccg acgatgagca gcgccttgcg ggcatcgagg ccgctcatgc tcgcgaagac
     2461 cgccgggtgc tcctcttgcc ggagggaccg gcgcgcgacc acgatgaacg ccagaccgag
     2521 cagggcaccc gccatcgcac gcagggggcc gtaattcatt ccctcgtaga ggagaccgaa
     2581 gctcgccgcg agcatcaacc cggccgcgac ggcgttggag gctccgagcc acctgcgggt
     2641 ggggtgcttc gcgaaagcga agggcacggc gccgagcccg gtggcaaccg ccgtgagcag
     2701 cgcgaagaga aagaccgaag ttgcgcccac cggttccata cacctacact actaggagcc
     2761 gctgtcgtcc gcgagctccc accctcagat cttggcctgt ggatagacca gcaacggcca
     2821 ggtaggagga acttcgcctt gggctgcggc atcgacctgc ctctccaacc cgcgctgagt
     2881 gagccatgcg tcccgaacgg cgtccttcac ctcggggatg ctcctcaagc cacccctcgc
     2941 gaaatctgcg gagaggacta tcaccgccct ggcgttcaac tcgacgagcg ccttacagat
     3001 cgcccggttc aaccttccct ctacagggta cgagacgcgt ctcgaccgta tcacctcagc
     3061 ccgctgccag ccatcctctc ctcgcttggt gacggcccgg atgtcccgca aggcgccgct
     3121 cgtccgcaag aactcctcgt gcgcccgctg cgtgagcacc agcccctcgt gaacgaggaa
     3181 acccgcgcag gcgagcaggt ccaagagctc gccacgcgcg ttgtttgggc cgtaccccgc
     3241 ctctccgaga cgccggacga actcgccctc cgccattcga aatcccttcc aactccgctg
     3301 tctgcgctac tatttccgtc tctccgcgac gaccgctccc gcgaagaatc ttctcgccat
     3361 tgtgagcagt tgccttaccg taccggacac aagcggcctc gccatgagca gggttgtcca
     3421 tgtgcctggc aaggattgat caaatgggac gttgataggc gtgagaggga gaggttagcc
     3481 gccgaggatg cccatctcgc gagccttggc caaaacctcg agctgggagt gcgcacccaa
     3541 cgcctgcaga agggagcgca cgtggtttct gaccgtggcc tcggaaatgt agagctcctt
     3601 accgatctcc ttcaccgact tgccctcaga tagcaatcgc agcacttcga gctgccttga
     3661 ggtcagggcg gggacgtctc tccttctcgg ggctggggcc tccttcttgg aagagagctg
     3721 aatcagcccg tgggccatct caagcgcgtc gtgggttccc tgggtgtcgc gaattaggtg
     3781 caccaggtat gtcccctcct cggtctcgag cgtaaggttg ctgacgctca cccatcgcct
     3841 gtctcccgag cgggtcttta tgcgcatctc gaaggctgag acgggacgat gaccctgagc
     3901 aaggtgcatc accgagcaac cgtgagcaca gaaaggtccc ccgtcctccc gctcgcccat
     3961 gacggcttcg taacagggct gtccaatggc ttcctgcgag agcgccccgg tgagggactc
     4021 catctgagaa tcccagtgga cgatcctgta gtcggggtcc accacgtaga cggcatcgcc
     4081 cgtgcgggcc tcgatcaggc tcctgaccgc tggaggaagc tctcgcaccc gcttatcgtc
     4141 gtccatcaca ggcctattgt atagtccgcc tccggaggat caatcgtcgc gagagccccc
     4201 gagacgttgg atcctgagcc gacaagttcc ttcgtaccat gctcggggcc tcgatccccg
     4261 accactcccc tttaccgacg ctcgggcatc caggagcggc cagctagccg caccaactcg
     4321 atgtctgtag tggcggacag gtctacaagt cctgcgctcc ccttggccgg gtcgaaagcc
     4381 agaagcgcca cctcgcccat ccgggcgacc gttccctgga tcgccacccc caccagctcg
     4441 cgctcgagct ccgcgagcgt cgggccaagg cgcgcgcctc ggataaacgc gcggccgggc
     4501 ataggggtcg gtcccaaggg gaaagccggg aagaacctct gttcccagat gtggcgggcc
     4561 tccacgcgag gaacgacgcg tccccgatga gattcgctcg ccctatcaag agcaggctcg
     4621 atccaggacg ccctctcttc cgggtaagca ccgaagagca cgtgcccttc ctcgagacca
     4681 ctggcacggg tcatggcggc attcaggaag ccaaggtgcc agagcggcgc accgccccga
     4741 tagagatcca agacagccgc cgcgagatcc tccacgtccc gaaaaacaac cccctcgggc
     4801 acgtccccgg cagccggcct cgtcgcgagg cgcgccctca ccaggaaccc catgctgccc
     4861 ctggatccta caaaatgcct cagagtttcg ccctcgatga gatctcgttc gccccccgcc
     4921 agcacgacct ccaatgagag gacgttctgt agcaaccatc cgtactcgaa ggagcccacc
     4981 ccaagcccgt tctcggccag ccagccacca acggtggacc tcggtgcgct cgtggggtag
     5041 accgtcggtc ctatacctct ctcgcgcagc cgctcttcga gaaccatcca cgtgaccccc
     5101 ggctcgacct ccacccggtc ctctcccgac tccgtaatcc gtatctcgcg catcgcgtcg
     5161 aaccgcaccg cgagagcccg cggcgccctt cccgaatttt gggcggtgcc tgcaccccgg
     5221 gctatcagcg gtatgcggta gcgggcggcg agtttcgtta gcgactcgac ctgtgcagcg
     5281 ttctctggga acacggaggc gaatagctgc ccggcatccg cttcgccatc cgcgtgccgc
     5341 acgaagctgt ttccgaaaat atcttcgatg gttctcagag ccacctcggg gatgtcgttc
     5401 ataagaggcc tttctaccat tcgtgcatca cgttcaacgc tccacccttt tgcccttcct
     5461 gtagcccgtc tgaacgggat tcatcggaat tttagaagat ggggcgactc gcggagccgg
     5521 gaagttgacc ggggtagcgc ggaaagctgc gtgcaggtgc caggccacaa gcacgactac
     5581 ggtgagcact gcaccgagga ccgcgagttc gactggagcg tggttgcgga tcgcctcggc
     5641 cgcgaggatc aggccggcta tgacggtcac cgcgagcgag atgccccagt ggaagcgacc
     5701 tgcctccgag tgcggccagg ggtccatggc gcgcacgact tcgcgcccca cgtcggagtc
     5761 cgtgcactcg gccttggccg ggcaatagtt gcagatctgg ggccttcccc ggtagcgcac
     5821 cagccgccgc tcgtgatcca cttcgctcct gacgaggagc tggtcctcag ggcagaccca
     5881 ggcgtcatgc gagggatggt aggtgaaccc ggcggtgcgg tagcgctcag agcgggcgtg
     5941 cgagtggcga gccaggaggt cgagcccgaa ggcgactccc agcaagaaga tcgcgtatcc
     6001 taccgcgagg agcacttcga gttggatatc catcatgcta ccgctccctc gacccggtgg
     6061 cttcgggctc ggtgatctcg gtgaagagta tatcctccgg ctcctccaca actacgcgcg
     6121 ggacggtgtg gcgcacgccg aggtagcaga gatacaacag cggcagcacg ccgccgacga
     6181 tgaagaccac gtcccccggc agacgaatcc actccaggag ggcgttcgtc tcgttggtca
     6241 ggaactctag cgtgcgggcc tcatagtaac cgacgctcac cgactggtag agctgcagga
     6301 tgcccaaagg gaagagcgtg gcgaagcaca tccaggcgag cccgatgttg agcgaccaga
     6361 agctcatcct ggccgcccgc tccgaccagc gttcctccgg gatcaggtac cggaggcaga
     6421 acagggccaa ccccgccgcg agcatcccgt agacgcccat catcgcggcg tggccgtggt
     6481 tggccgtcaa ggccgtgccg atctgatagt aggagatgat cggcaagttt atgaggaagc
     6541 cgaagatccc cgctccgagg aagttccaga accccaccgc aacgaggaac atcaccgccc
     6601 agcggtgcgg gaacggcgtg ggagagcgtg actcctgcct ggccccgagc tgcaaaaagc
     6661 tccaggcctc tacggttagg aaggtgagcg ggatgacctc cgccgccgag aagaaagcgc
     6721 caagagccat gtgctcggcg ggcgttcccg aaaagtagag gtggtgcatc gtcccgatca
     6781 cacctcccgc cgaatacaac aagatatcca ggtagatgac ggtcagtgcc accttctcgc
     6841 gcacgacgcc gagcaaaaca aatatatagg cgaccatgac ggtcgtgaag agctcgagga
     6901 agtcctcgac ccataggtgg acgacccaaa accgccagaa atccgccgtc gtgaagtgag
     6961 actccggccg ggcgagcaac ccgaccgcgt agaacgccgg gatcgcaagg gcggcgaaga
     7021 agaacagcca gggcatgttg cccatgtgct cgcgccgcaa ccgcccgcgc atcgcccgaa
     7081 acagcatcac gacccagaag aagagaccta tcgagagcag gatctgccag aagcgcccga
     7141 ggtccagata ttcgaagccc tggttgccga accaggccca aaggccgccc gtcaggccga
     7201 agatccccgc ccactcccca atgaggctcc cgaagacgac tatagccaga gcgcctaata
     7261 acccgaaggc gaggaggcgc tggcctcgtg gctccctgcc ggcgatcatc ggggcgagga
     7321 agatgcccgc cgctagaaag gaggttgcca cccaaaagat cgcgagctgt acgtgccagg
     7381 tgcgcatgag gttatacggc aaccactgcg cgaggtctat gccgaagaaa ctcgccaggt
     7441 ccgctcggta gtgctgcgac gccgccccca cgagcgtctg tacgaggaac agagcggcca
     7501 tcaccagaaa gaaccatgcg cacgcccgct gcgccggcgt cagcgccacg tcgccgggtg
     7561 tgcgaaacga taacgtcgcc tgctcgcgcc cccgccagcc gaggaagttc caccgcccaa
     7621 aggctgcgaa caagagcccg atgccgccga ggagcgctat gagcgaaagt acgctccaca
     7681 ccacgacgtt ggcggtcggc tcgttctcca cgagcggctc gggcggccag ttgttggtgt
     7741 acgtgtagtc cgtccccggc ctcaacgccg acgcggccca cgccgtccag gcgaagtacg
     7801 cggttagctg tcttatatcc tcggggtctt ttatcgcctc cggcctgagt ccgtacttcg
     7861 tagtcggttc gccgaagtac cgcccgtaat gctcctcgag ctcctcgaag gccgcagcct
     7921 gcggcgcggt gaactccagc gtgcgtgtct ccgggtcgta gcggttctcc ttgaagtcgg
     7981 agacagtctg caccgccgcc cggtcggagc tctctccccc ataactctcg cgcacgaaga
     8041 gggcggctcg ccgcagataa tcagcggtga agtcggggcc gaggtaggca ccgtgcccga
     8101 agatggaacc gtactgcatc aagccattct gcaggaacac cccttgcccg gccctcacgt
     8161 cctcgccggt gaagacgacc tcgcccccgg ggtccacgac ccgctcggga atcggcggct
     8221 gccccgaata cgcccgatac gccagaagcc cgagcacgaa aaagccaaac agaacaacca
     8281 gcgccacggc ctgaacccag cccttcgaga taagcagctc acgccgctcc gatcctccta
     8341 ccaccagcca cctccagctg atcccggccg gggtagccat cccaccccga aaatattacc
     8401 aatttagtaa tataacttta gctctgacca tcacgcaagg tgtatggcgt gggagcctgc
     8461 tacgctcttg ggaacttgtc ggaggcggtt cgcgagatga actccttgat ggtctgcgtg
     8521 ccgaggtgct cgaggatagc tttctggccc tctacccagg cgtcgtgcag ggcgcatgga
     8581 gcgccctctc cgcaggcgcg atcctccata atgcagcgga cgatgctgaa tggcccttcg
     8641 accgtatcta cggcttcccg aagcgttatc ttgttcgcgg gtcgggcaag ccgggaacct
     8701 ccgccgcggc ccttttcggt ctcgacgaga cccgcgtggg agagcttggc caggatgcga
     8761 gcgagcaggc ggcggggaat gtgggcttcg gcggagatcc tgtcggcggt cacccgctca
     8821 ccgacccagg caagatacac cagagccctc aaggtgtacc gcccttcgct actcaactcc
     8881 aacttcaaac tcgccggctc ctttggaggg aatacttggc cgctccgatc gaacgttgcc
     8941 cgctaatgcg cacctcccta ccgcgagcgg cctcttctac cactagatct gtaatgatat
     9001 gtgaagaata aggtcaagtt tcgcgtgtgg gcgagtatgc cgcgcctcat ccgatctcgc
     9061 cggtcgtctg cgaagaccgc attcgctcca gaataggaga gaggtcggac cggggaccat
     9121 actcgcgtgc gtggccggag tacgccgcgg cacgtcgaag ctttcagcca catagtctgc
     9181 ctcgtccaaa ttgaccgtct tccgaggtgc cagcacccga tcggcggggg ctttttgatc
     9241 atttctagct agaagcatga tccaatttgc tcagggcgtc gtgtgcgccg caggttacag
     9301 tgatccctga tgagcaaacg gcgaacggga ccggaacacg agcccaacag aggtctcgag
     9361 gcctatctat accgttagga ctgagtggga ggttgtgctc tggagccacg tagccaggtt
     9421 acttggtgcg cgagcagtca ggagagcgag gaggtaagga atgggcgaac gtcacg
//
LOCUS       DASTKM010000232         6746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_423710, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000232 DASTKM010000000
VERSION     DASTKM010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423710"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFQ10_09585"
     CDS             complement(<1..186)
                     /locus_tag="VFQ10_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965634.1"
                     /translation="MDRGQEIIGLNGAAAVLDVSSETVRRFAKEGRLRSEGGEEGGYV
                     FHLEDVLGLARELGAESA"
     gene            complement(301..819)
                     /locus_tag="VFQ10_09590"
     CDS             complement(301..819)
                     /locus_tag="VFQ10_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET9965635.1"
                     /translation="MERISRERFIRLGTALGVGAGCASLVACGEGASSSGGGSGGGKS
                     GGGDSGGGNYGGGNSGGGGSKKSSGGEAKTGGTAIASESQVAPGSAYKFEDSGNPALL
                     VHLENGDFVAYSAVCTHQACKVAYQDGNLVCPCHGSTFDPANGASVVTGPATRPLPEI
                     PVKVEGGEVVRA"
     gene            1051..1536
                     /locus_tag="VFQ10_09595"
     CDS             1051..1536
                     /locus_tag="VFQ10_09595"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HET9965636.1"
                     /translation="MVLVRVLIGAVWLNGGLEKLLNPGFPGQFAASLQAGGFVSQAPP
                     FFQDFMRGYVVPNAELFAQFMRAGELMLSIALILGLLTNLAAIGSVGLSAVILLSQGG
                     VGLGTGLGAPEFLTINVLVALISVVILFSPAAKTISLDSRLAERSPGLSLLLVNRRQE
                     D"
     gene            complement(1545..2795)
                     /gene="lysA"
                     /locus_tag="VFQ10_09600"
     CDS             complement(1545..2795)
                     /gene="lysA"
                     /locus_tag="VFQ10_09600"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET9965637.1"
                     /translation="MDDFHYEGGVLRCEDVPLSEIAASAGTPTYVYSHAALQRAFTEL
                     DGAFAGLDHLVCYAVKANGNLAVLRTLAALGAGADVVSGGELYRVMRAGFDPKKVVFA
                     GVGKTEKELMSGLGERILLFNVESASELEHLDRLCARFGKRARVALRVNPDVDAGTHD
                     HVATGRGQDKFGIPVDEALALAERVGDYRCIDLIGVHQHIGSQITKLTPYTESVERSA
                     GLVDELKTRGFDIRYFNIGGGLGIPYKDEETPTPKELVDAIRPMLEAAGTKILCEMGR
                     YISGSAGVLLSSVVYRKKSGGKSFIVADAGMNDLLRPSLYDAHHEVLSVDEVGSVANA
                     DLVGPVCESGDYLARDRELPDASEGDVLAIMDAGAYGFSMASNYNSRPRPAEVMVKGD
                     SWAVVREREHTADLVKGELVPAFL"
     gene            2871..4055
                     /gene="dapC"
                     /locus_tag="VFQ10_09605"
     CDS             2871..4055
                     /gene="dapC"
                     /locus_tag="VFQ10_09605"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR03537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HET9965638.1"
                     /translation="MPNPRATHQTQKHPLLLNPILAKQGVYPLLHLDERRWELEKKGL
                     DLFDFGTGDPREPTDAKIRHALIDGVPEVSRYPSTAGKQGLREAFCGWMERRHGVALD
                     PESEVLPATGSKEAIFHAPLAFLHHSHERRGVAYGTPGYPVYERGTLLSGGEALPVEL
                     KGEDGFLLQPEAIDGERTRILWINYPHNPTGATATYKYLEGVVEFCREHGILLFSDEC
                     YNDIFSGEPPPSILELTTERTLAFCSLSKRSGMTGYRSAMMAGDPELIGALRKLRPSI
                     GVATQSFVQDAARAAWDDDAHVEKRRRVFGEKRDLFTAFFEEAGLEYLPTEASFYLWV
                     AIPGGDDEAYALRLMEEGVIVAPGRAFGAGGEGYIRIALVPGLEDCKLAIQRWEGIEG
                     LA"
     gene            4052..4570
                     /locus_tag="VFQ10_09610"
     CDS             4052..4570
                     /locus_tag="VFQ10_09610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9965639.1"
                     /translation="MRTRDATLEDLASIVEIYNSIIPGRIVSADSEPVSVEQRLPWFY
                     EHDPARRPIWVAEEDGEVVGWLSLGDFWDGRPAYNGTAEIGIYVKEGHRGKGIGRRLL
                     EEAIRRAPDLGIKTLTAGAFAGNEPSLRLFERFGFEKWAHFPRVAELDGAEIDLVVLG
                     RRLEAGTANHSL"
     gene            4765..5874
                     /locus_tag="VFQ10_09615"
     CDS             4765..5874
                     /locus_tag="VFQ10_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HET9965640.1"
                     /translation="MAENWGDEMRLVRIEDLRHCLIAAFETLELPREDAKQVAEVLLD
                     SELRGHESHGVHLLGFLANAYHGGGMNPQPRIRVLRETEGALLLDGDRSHVVGAARAM
                     RWCIERAREHKGMAGAGVRNSQLIVPGFYARMAAEAGLIGFACANAVPMVAPPGGRTP
                     TFGTNPFAYAIPAGRYPPVVLDVATTTGAAFKVRLAAQRDRPVPEGMILDGEGRPTTD
                     PNEFVRGGLMAPLGSPAAPHKGFGLGLVFDALAGVLTGAAFARDFPSEPATAGSAFFW
                     ALDVEAFLPREEFLGRMEAQIDQVKAGERLAGVDELFLPGERSHRRYRELTTRGTAPL
                     SVATWEALTKACASLTIAPPPALAAERRQSDSELT"
     gene            6137..6505
                     /locus_tag="VFQ10_09620"
     CDS             6137..6505
                     /locus_tag="VFQ10_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965641.1"
                     /translation="MYEQITVEIDRGYGEGNYRSYSINFIGRLIRIVEAGHTKMRLYE
                     WADLGQLLYLVHVEEDKPGMPPRRTLYPFINTLSAGAIPRKGYTAGEVSRAYPELAAG
                     RVLGNLQNSRQRGRDHIRTW"
BASE COUNT         1207 a         2212 c         2111 g         1216 t
ORIGIN      
        1 cgcggattcg gcgccaagct cacgcgcgag cccaaggaca tcttcgaggt ggaagacgta
       61 tcctccttcc tccccgccct cggagcgaag cctcccctct tttgcgaagc gcctgaccgt
      121 ctcggacgag acgtccagaa cagccgccgc cccgttgagc ccgatgatct cctgccccct
      181 gtccatgaga tcattctact ttagctttca gcggtcagcc atcaaccatc agcttttcgg
      241 tggatagaga acgacttgtc agcgaggcgg gaccgccctg aggtctttag ctcgctgctc
      301 tcaagctctg acgacctccc cgccctcgac cttcacgggg atctcgggca gcggcctcgt
      361 ggccgggcct gtaaccaccg aggcaccatt ggccgggtcg aaggtcgagc cgtggcacgg
      421 gcagacgaga ttgccgtcct ggtaggccac cttacaggcc tgatgggtac agaccgccga
      481 gtacgccacg aagtccccgt tctccaggtg gaccaggaga gcaggattcc ccgagtcttc
      541 gaatttgtag gcactccctg gtgcgacctg ggactcgctc gcgatggccg ttccgcccgt
      601 cttggcttcg ccgccggacg atttctttga gccgccgcca cctgaatttc ctccgccgta
      661 gttgccgcca ccggagtccc cgccgccgga ctttccaccg cctgaacctc cgccggagct
      721 gctcgcgcct tctccacagg cgaccagcga ggcgcatcct gcgcccactc caagcgcggt
      781 gcccagcctg atgaacctct cgcgcgaaat cctctccaag gcttcctcct agttgtcgcc
      841 gggttcccct tgatatacgc agcgtatcca gcattggttg tgtttagcac ttcagcaagt
      901 cagcacgccg ccttgcaggc ggcttgtcag cacgctccag cctcaggctg tcgcttgtca
      961 gcatttcggc tagatcctaa actctctccg gcgcgtatgt aggataggag cgcgtggcag
     1021 tgtgtgagag gagtccggca cgtgaacggc atggtgttgg tgcgcgtcct gatcggggcc
     1081 gtctggctga acggtggcct ggagaagttg ctcaacccgg gcttccctgg ccagttcgcg
     1141 gcgagccttc aggcgggggg tttcgtctcg caggctccac ctttcttcca ggattttatg
     1201 aggggctatg tagtgcctaa cgccgagctc ttcgcccagt tcatgcgcgc gggagagctg
     1261 atgctaagca tcgcccttat cctcgggttg ctcaccaacc tcgcagcgat cggcagtgtc
     1321 gggttgagcg ccgtgatcct gctctcccag ggaggcgtgg ggctcgggac tggcctcggc
     1381 gcccctgagt tcctcacgat aaacgtgctc gtggcgctta tctcggtggt gatcctgttc
     1441 agcccggccg cgaagaccat ctccctggac tcccgcctcg ccgagcgcag ccctgggctc
     1501 tccctcctgc tcgtcaaccg tcgccaggaa gactagccta gcacctacaa aaacgccgga
     1561 accagctcgc ctttcacgag gtcggccgtg tgctcgcgtt cgcgcacgac ggcccagcta
     1621 tctcccttga ccatgacctc tgcgggcctg gggcgggagt tgtagttgga ggccatcgag
     1681 aacccgtaag cgccggcgtc catgatggcg agcacatctc cctcggaagc gtctggcagc
     1741 tcgcggtcgc gggcgaggta gtcaccggac tcgcacacgg gcccgacgag gtcggcgttg
     1801 gccacggagc cgacttcgtc tacggagagg acctcgtggt gggcgtcgta gaggctcgga
     1861 cgcaggaggt cgttcatacc ggcgtcggcg acgatgaagc tcttcccgcc gctcttcttg
     1921 cggtagacga cgctggacaa gagtactccg gcggagccag atatatagcg gcccatctcg
     1981 cacaggatct tcgtccccgc cgcctcgagc atgggcctga tggcgtccac tagctccttc
     2041 ggggtcgggg tctcctcgtc cttgtagggg atgccgagac cgccgccgat gttgaagtag
     2101 cggatgtcga agccgcgggt cttgagctcg tcaacgaggc cggcggagcg ttcgacggac
     2161 tcggtgtaag gggtgagttt ggtgatctgg gacccgatgt gctggtggac cccgatgagg
     2221 tcgatgcacc ggtagtcccc tacacgctcg gccagggcca gcgcctcgtc cacggggatg
     2281 ccgaacttgt cctggcccct tcctgttgca acgtggtcgt gcgtcccggc gtcgacgtcg
     2341 gggttgacgc ggagcgccac gcgggccctc ttgccgaagc gggcgcacag gcggtcgagg
     2401 tgctccagct cggaggccga ctcgacgttg aagagcaata tgcgctcccc aagccccgac
     2461 attagctcct tctcggtctt cccgaccccg gcaaagacga ccttcttcgg gtcgaatccg
     2521 gcccgcatca ccctgtagag ctccccgccg gagacgacat ctgctcctgc cccaagagct
     2581 gcgagcgtcc gcaacacggc gaggttgccg ttcgccttga cagcgtagca gaccagatgg
     2641 tccaggcccg caaacgcacc atcgagctcc gtaaatgccc tctggagggc ggcgtggctg
     2701 tagacgtagg taggggtccc tgccgacgcc gctatctcgg agagcggcac gtcctcacag
     2761 cggagtacgc ccccctcgta gtgaaagtcg tccaaaacgt acctcctcgt gtagccggag
     2821 cgtgctatat ttctggctct gcaaatggag ccgtagggag aatatactcc gtgccgaacc
     2881 cccgcgcgac acaccagaca caaaagcacc ctttgctcct caaccccata ctcgccaagc
     2941 agggcgtcta cccgcttttg cacctcgacg aacgccgctg ggagctggag aaaaaggggc
     3001 ttgatctctt cgacttcggc accggcgacc cacgcgagcc gacggacgcg aagatccgac
     3061 acgccctgat agacggcgtc cccgaggtca gcagataccc gagcacggcg ggcaagcagg
     3121 ggttgcgcga ggccttctgt ggctggatgg aacgccgcca cggggtcgcg ctcgaccctg
     3181 agagcgaggt tctgccggcc acaggctcca aagaggcaat cttccacgct cctcttgcct
     3241 tcctgcacca ctcgcacgag cggcgcggcg tcgcctacgg cacgcccggc taccccgtct
     3301 acgaaagggg cacgctgctc tccggcggcg aggcgctgcc tgtcgagttg aaaggtgagg
     3361 atggtttcct gctgcaaccc gaggcaatag acggcgaacg cacgcggata ctctggatca
     3421 actaccctca caaccccaca ggcgcaacag caacgtataa atacctggag ggcgtagtcg
     3481 agttctgccg cgaacacggc atcctgctct tctcggacga gtgctacaac gacatcttct
     3541 ccggcgagcc cccgccttcc atcctggagc tcactacgga gcggacccta gccttctgtt
     3601 ccctgtccaa gcgcagcggc atgacaggct accgctcggc catgatggcc ggagatcccg
     3661 agctcatcgg cgcgctgagg aagctccgtc cctccatcgg cgtggccacc cagtcctttg
     3721 tccaggacgc cgcgagggcg gcctgggacg acgacgccca cgtcgagaag cggcggcgcg
     3781 tcttcggcga gaagcgggat ctcttcaccg cgttcttcga ggaagccggc ctggaatatc
     3841 tgcccaccga agcgagcttc tacctgtggg tcgcgatacc cggtggggac gacgaggctt
     3901 acgcgctgcg cctgatggag gagggagtaa tcgtggcgcc cggccgggcc ttcggggctg
     3961 gcggcgaggg gtacataagg atcgcgctgg tacctgggct cgaggattgc aagctggcga
     4021 ttcagcgctg ggaggggatc gaagggctgg catgagaacg cgtgacgcca cgctggagga
     4081 tctcgcgtcc atcgtggaga tctacaactc catcatccct ggccgcatag tgtcggccga
     4141 ctcagagccc gtctccgtcg aacagaggct gccgtggttc tacgagcacg acccggcgag
     4201 acgtccgatc tgggtggccg aagaggacgg agaggtagta ggctggctca gcctcggcga
     4261 cttctgggac ggcagacccg cctataacgg gaccgccgag atcggcatct acgtcaagga
     4321 gggccaccgg ggaaagggga taggccggcg cctgctggaa gaggctatca ggcgtgcccc
     4381 ggatctgggc ataaagaccc tgaccgcggg ggccttcgcc ggcaacgagc ccagcctcag
     4441 actgttcgaa cgcttcggct tcgaaaagtg ggcgcacttc ccgcgcgttg ctgaactcga
     4501 cggcgccgag atagacctcg tcgttctcgg acgcaggctc gaagcgggaa cagcgaacca
     4561 cagcctttga ctctgtcgaa atccccagcc tggtcccttc tcggcatttg cgtgaacaag
     4621 gcaagatgac cgtcggccag gcgtcaccca aaagctcatt cactaacgct cggagacaaa
     4681 cgggaggctg ccagagagtg cgcgggagtg cgaagagctt gctacgatgc acgcaatccc
     4741 atacggtgaa aggggaggat ggccatggct gagaattggg gcgacgagat gcgcctggtt
     4801 cggatcgaag atctgcggca ttgcttgatc gcggcgttcg agacactcga gctgccgcgc
     4861 gaggatgcca aacaggtagc tgaggtcttg ctggactcgg aactgcgtgg ccatgagagc
     4921 cacggcgtcc acctactcgg ctttctggcc aatgcctacc acggtggagg catgaacccg
     4981 cagcctcgca tccgggtgct gcgggagacg gagggagcgc tgctcctcga cggggaccgc
     5041 agccacgtgg ttggggccgc gcgggcgatg cgctggtgca tcgagcgcgc ccgcgagcac
     5101 aagggcatgg cgggcgctgg cgtccgaaac tcgcagctca tcgtgcctgg gttctacgcc
     5161 cggatggctg ccgaggccgg cctgatcggc ttcgcttgcg ccaacgccgt cccgatggtt
     5221 gcaccgcccg gcggccgcac gccgaccttt ggcaccaacc cgttcgccta cgctatcccg
     5281 gcaggacgct acccaccggt ggtactcgac gtggccacga ccaccggcgc cgcgttcaag
     5341 gtccggctcg cggcgcaacg cgaccgtccg gtgcccgaag gcatgatcct cgatggcgaa
     5401 ggccgtccga ccaccgaccc gaacgagttc gtccgtggtg ggctcatggc accgctcggt
     5461 tccccggcag cgccccacaa gggcttcggc ctggggctcg tcttcgacgc gctggccggc
     5521 gtcttgaccg gggccgcatt cgcccgcgac tttcccagcg aacccgccac ggccggcagc
     5581 gccttcttct gggcgctcga cgtggaggca ttcctaccgc gcgaggagtt cctggggcgg
     5641 atggaggccc agatcgatca ggtcaaggca ggcgagcggc tggcaggcgt cgatgaactg
     5701 ttcttgccgg gcgaacgtag ccaccgccgc taccgcgagc taaccacccg cggcacggcg
     5761 ccgctctcgg tggcgacctg ggaagcgctc acgaaggcct gcgcatccct gactattgca
     5821 ccgcctcccg cccttgcggc tgagcgcagg cagtcagaca gcgagttgac ctagcatcgc
     5881 aacgactagc gctcgcagaa gatcactggc actaaacagc cagagtatgc cgtgcattgc
     5941 cttgagatgg tctatccact gttcagaaat tcgcctcagg caggatagct actcccgact
     6001 ctacaagcct taacgcaacc ttcgccgacg cttaactgcc tgtgacactc ggggtgttag
     6061 ggtctcggta accgcgggat tcggccgggg ctgtccccct ccgttccggc cggaccctcg
     6121 ggctgaggag aaacgcgtgt acgagcagat caccgtagag atcgacagag gttacggcga
     6181 ggggaattac cgttcgtaca gcatcaactt catcggacgc ctgattcgga tcgtcgaagc
     6241 tggccacacc aagatgaggc tctacgagtg ggcggatctg ggccaattgc tctacctggt
     6301 ccacgttgag gaggacaaac ctggtatgcc tccccgacgc accctctacc cgttcatcaa
     6361 caccctctcc gccggtgcca tccctaggaa gggatacacc gcaggggagg tatctcgggc
     6421 ttacccggaa ctcgccgccg ggagagttct gggtaatctc cagaactcgc gccaacgagg
     6481 tagggatcac atccgcacgt ggtagcgact tcgagtagcc gccgagcgca ctcgttagac
     6541 gtagggaagt gctcctcgag cttttcgcga cttcactctg gaaccgtagt aagggtaagc
     6601 aacgacgcct aacgcaacgt gattctctca ggggaccggc gtttcttctt tggctgcctt
     6661 tcctctcttg tgccaggctc acctatcacg aaccagtacg caccgatcag cataatcagg
     6721 aacaggagca gcgccacggc gaactc
//
LOCUS       DASTKM010000233         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_424097, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000233 DASTKM010000000
VERSION     DASTKM010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424097"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFQ10_09625"
     CDS             <1..467
                     /locus_tag="VFQ10_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET9965642.1"
                     /translation="RRIEGGILALGCDMWYDTNPYEVGYGYKWMVELEQEQHFVGREA
                     LKRISEEGVSRKLVGVELGGSNLGSYNDGSMIDYFPVHKHGERIGKVTSACYSPRLQK
                     NIGYAMVPVEYSELGTGLEVERPEETVAATVADRVFFKPEKAEQNLGAASAS"
     gene            495..1382
                     /locus_tag="VFQ10_09630"
     CDS             495..1382
                     /locus_tag="VFQ10_09630"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET9965643.1"
                     /translation="MGKNVTTEQNPELSQLANRRLVEFLRHPGYEVIPLQGTEESVIE
                     HVPKEIALTVTASPGKGTWATVELAERLAGHGFSVAPHLAARLIRDRSELGEILQRLR
                     EASIRNAFVVAGDVDEPAGEFEGAAGLLQAMSEIGHDLDEVGITGYPESHPKISDEMT
                     IQAMYEKSSYATYIISQICFDPGVTLDWARRVWLRGVKLPIRVGMPGYVNRQKLVRIS
                     AGIGLGESARFLNRQRNWLLKLFLPGGYSPDRLIEGLKPGFADPESNIQGIHIYTFNE
                     VQRTEAWRREMLERAETSA"
     gene            1398..2006
                     /locus_tag="VFQ10_09635"
     CDS             1398..2006
                     /locus_tag="VFQ10_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HET9965644.1"
                     /translation="MDFSPENTTSEYLDLPLGEFLDLVASGNPAPGGGSVAAVAVALA
                     AGLSGMAARLSAGQLADADGLAERADAARRRVAPLARIDVESYRRVLDAYREPDSETR
                     RERVSDALSGATDVPLAVAEVGNEVAGIAARLVEEGNTNLVGDAITAVLLAEAGVRAA
                     VTLAEINLSAANLDDDRLGRANELVDETASTARRITGGHRRG"
     gene            2065..>2585
                     /locus_tag="VFQ10_09640"
     CDS             2065..>2585
                     /locus_tag="VFQ10_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HET9965645.1"
                     /translation="MPERRTPFYDFHRRSARNLVKGGGDFMFPTSYASPLEEHLNVRR
                     NVGMQDLSTMGEVDIKGPGAERLVRRLLVNEVQDMEPGQLRYSTMCNEAGGIVDDVTV
                     YKFEDEHFMIVTSSGPREKVFRWISEHAVGSSAYVTDMTAAVALPVVQGPRSRAFLKT
                     LVEGVDLDELRFFR"
BASE COUNT          517 a          750 c          882 g          436 t
ORIGIN      
        1 tccgtaggat cgaggggggc atcctggccc tgggctgcga catgtggtac gacaccaacc
       61 cctacgaagt tggatacggt tacaaatgga tggtcgagtt ggagcaggag cagcacttcg
      121 tcggaaggga ggcgctgaag aggatcagcg aggagggcgt cagccgcaag cttgtcggcg
      181 tcgagctagg gggtagcaac ctcgggtcct acaacgacgg ctccatgatc gactacttcc
      241 cggtccacaa acacggcgag agaatcggca aggtcacctc ggcctgctat tcgccgcgcc
      301 tgcagaagaa catcggctac gcgatggtgc ctgtcgagta cagcgaactg ggaaccggac
      361 tcgaggtcga gcggcccgag gagacggtcg cggccaccgt ggccgaccgc gtcttcttca
      421 aaccggagaa ggccgagcag aacctgggcg ccgccagcgc gtcttaaggg accagagaag
      481 agcgactcgg gttggtgggg aagaacgtga cgacggagca gaaccccgaa ctgagtcagc
      541 tggcgaaccg gaggctggta gagtttctgc ggcatcccgg ctacgaggtg atacccctcc
      601 aggggaccga ggaatcggtc atcgagcatg tcccaaagga gatagcactc acggttacgg
      661 catcgcctgg taagggaacc tgggccacgg tagagctcgc cgaacggttg gcgggacacg
      721 gcttcagcgt tgcgccccac ctcgcggcgc ggctcattcg ggacaggtcg gaactgggcg
      781 agatcctgca gaggctgcgc gaggcctcca taaggaacgc cttcgtcgtc gccggcgacg
      841 tcgatgagcc agcaggggag ttcgagggcg cggccgggtt gctccaggcg atgtcggaga
      901 ttgggcacga cctcgacgag gttggcatca caggctaccc ggagagccac cccaagatca
      961 gcgacgagat gacgatccag gccatgtacg aaaagtcgtc ctacgccacc tacattatca
     1021 gccagatctg cttcgaccct ggggtgacct tggactgggc caggcgggtg tggttgcggg
     1081 gtgtaaagct gccgatccgc gtcggtatgc caggctacgt gaaccgccag aagctagtgc
     1141 ggatctccgc cggcatcggc ctcggcgagt cggcgcgttt cttgaacagg cagcgtaact
     1201 ggctgctcaa gctattcttg cccggtggtt acagccctga ccgtctgatc gaaggcctga
     1261 aacctggatt tgcggaccct gaaagcaaca tccagggaat ccacatctac accttcaacg
     1321 aggtgcagag gaccgaggcg tggcggcgtg agatgctgga gcgcgccgaa acgtccgcct
     1381 gatgggcttg tagtaggatg gacttttcac cagagaacac gacgtcggag tatctggatc
     1441 ttccacttgg agaattcctt gacctcgtgg cctcgggcaa cccggctccc ggcggaggct
     1501 ccgtggctgc cgtagccgtg gccctcgccg ccgggttgtc gggcatggcc gcgcgcctgt
     1561 ctgcgggcca gctagccgac gccgacgggc tggccgagcg tgcggatgca gcgaggcgga
     1621 gggtagcacc gcttgccagg atcgacgtgg agtcctacag gcgcgtcctc gacgcttacc
     1681 gagagcctga ctctgagacc cgcagggagc gcgtcagtga tgccctctca ggggccaccg
     1741 acgtgccgct ggccgtcgcc gaggtcggga acgaggtcgc cggcatcgcg gcccggttgg
     1801 tggaggaggg caacaccaac ctcgtgggag acgcgataac cgcggtcctg ctcgcagaag
     1861 cgggagtacg cgccgcggtg acattggccg agatcaatct gtctgcggcg aacttggacg
     1921 atgaccgtct cggacgggcg aatgaactcg tcgacgagac cgcgtcgacc gcccggagga
     1981 tcacgggagg ccatagacga gggtgaccgg gaccagtgct tgcagtatag tttacaaaca
     2041 atcttcccga gagaggggta gttcatgccg gagagaagaa cgccattcta cgatttccac
     2101 cgccgttcgg ccaggaacct ggtcaaggga ggcggggact tcatgttccc aacctcctac
     2161 gcttcgcctc tagaagagca cctcaacgtg cgcaggaacg tcgggatgca ggatctctcg
     2221 acgatgggcg aggttgacat taagggacct ggcgccgagc ggctggtgag gcggctgctg
     2281 gttaacgagg ttcaggacat ggaacccggg cagctaaggt actcgacgat gtgcaacgag
     2341 gcgggcggca tcgtagacga tgtcaccgtc tacaaattcg aggacgagca cttcatgatc
     2401 gtcaccagtt ccgggccgcg tgagaaggta ttccgttgga tctccgagca cgccgttggc
     2461 tccagcgcct acgtgacgga catgacggcg gccgtcgccc tgcccgtggt ccagggaccg
     2521 cgttcgcgtg ctttcctcaa gaccttggtc gaaggcgtgg acctcgacga gctaaggttc
     2581 ttccg
//
LOCUS       DASTKM010000234         6030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_424236, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000234 DASTKM010000000
VERSION     DASTKM010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424236"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFQ10_09645"
     CDS             complement(<1..802)
                     /locus_tag="VFQ10_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965646.1"
                     /translation="MGELTLEYGVYSLITDPGALPAGVWTAWFGAWARGIGWFVIVTF
                     LLLLYPTGRLPSPRWRPVLWGTAGSVVLFTMASWLSPETNDLRLAAVRNPLGLEFEIM
                     GLLYELFNYAFPLLIVASGTAVIVRFRRSRGDERQQLKWFAYAVAVMIVVFVIWFSFT
                     LVGLVSPTSLLYEVPLIGLPVATGIAILKYRLYDIDFIVNHTLVYGSLTALLVVIYFV
                     GIVLLQRLFVALTGQQSTLAVVASTLLIAALFNPLRRSIQSFIDRRFYR"
     gene            complement(806..1036)
                     /locus_tag="VFQ10_09650"
     CDS             complement(806..1036)
                     /locus_tag="VFQ10_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965647.1"
                     /translation="MSHRAGAWFAWSVWALTVPTTILTLIFASLNEPSPSLWDEVLLP
                     VLILTFSTVGALVASYRPEKAIGWLFLTGAFV"
     gene            complement(1301..2431)
                     /locus_tag="VFQ10_09655"
     CDS             complement(1301..2431)
                     /locus_tag="VFQ10_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9965648.1"
                     /translation="MLLAANLRPALTGVAPLIGQIRADTGISNGVAGLLTTLPLLAFG
                     LLSPIAPRVARLFGMERVLLASLLVLVAGILLRWAGAVEALFLGTVVLGAAIAVANVL
                     LPSLVKREFPEHAGLMTSVYSSSLGISAALAAGVSVPLAQLVGWRGALAVWVLPALLA
                     GVAWLPQLSRSDHPGDPSARSTPGVRDLWRSPLAWQVTLFMGLQSLSYYVTLTWLPEI
                     LQAEGMSAARAGWMLGLSQAVAIVTMFLTPMIAGRRPSQRGVVVVAVGMSGVGALGLL
                     VAGSTVSTLWVVLLGLGQGAAFSLALTFFALRAPDPGHAAALSGMAQSVGYLLAAGGP
                     FLFGLLRDVTGAWKVPLALLLAVTMCLLIAGIGAARDAHVGT"
     gene            2788..3423
                     /locus_tag="VFQ10_09660"
     CDS             2788..3423
                     /locus_tag="VFQ10_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HET9965649.1"
                     /translation="MSEPEDLVRTITEEGVRDPRLLEALRAVPRAGFVPANLAGQAYE
                     DKPLPIPHDQVTTQPSLVARMVDALGLAGSERVLEVGAGYAWQTALLARLGGFVWAVE
                     RFGDVADAARENLARFGVTNAEVVTVDGTKGLPEHAPYDAILVAAAFLSVPRPLEEQL
                     AAEGRLVQPVGPGGREEVVLFQKGTRGLVRRRTIALARFVRLHGAHAFPEQ"
     gene            complement(3477..4520)
                     /locus_tag="VFQ10_09665"
     CDS             complement(3477..4520)
                     /locus_tag="VFQ10_09665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET9965650.1"
                     /translation="MRPVRHVSGRSSGTWGAMITLALTGDVMLGRGVAEVLDNRMRPE
                     APWGDVMPLLDAADLRIINLECAITDNERPWTRTPKVFHFRALPSAIETLRTARIDAC
                     SLANNHTLDFEEQGLLDTMEHLDAAGIRHAGAGRNREEAADPAILTVPADHTRRVALL
                     AFTDNEPPFAAGIDRPGTNYLPVSLKPEVLRRVERTVSYVREMGVGTIIFSNHWGPNM
                     VQRPKEIFRRFARAVIDRGVDIYYGHSAHVFQGVEIYRGKPILYDTGDFIDDYAVDPK
                     FRNDWSFLFQVSMEKGRFERLDLTPVKLSYARVDLATGGEPETILDRMERLSAEMGTV
                     FARREGALVFERG"
     gene            complement(4786..5028)
                     /locus_tag="VFQ10_09670"
     CDS             complement(4786..5028)
                     /locus_tag="VFQ10_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965651.1"
                     /translation="MNQERFKDLNLVPPEEGFELYRYKNAEKMRTSGWSGLYWARKNE
                     AGDYEIRAVTAEGEGYSYPGGVFSEEAFERYYEKAN"
     gene            complement(5126..5383)
                     /locus_tag="VFQ10_09675"
     CDS             complement(5126..5383)
                     /locus_tag="VFQ10_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET9965652.1"
                     /translation="MGVLSWIVFGLIAGIIAKLLMPGRDPGGCIITMLLGVAGAFVGG
                     FFYRLVTGTEVLYFGFDFRSFAVAVLGTVVVLAVYRLIIRS"
     gene            5584..5895
                     /locus_tag="VFQ10_09680"
     CDS             5584..5895
                     /locus_tag="VFQ10_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET9965653.1"
                     /translation="MTEQQVGQAHGYIKEGDKEKLRARLRRIEGQVRGVQRMIDEEKY
                     CVDILTQISSYIRASEKVALLVLRDHMDHCVREALADGENAHEKVRELQEAMERFLKL
                     D"
BASE COUNT         1268 a         1840 c         1893 g         1029 t
ORIGIN      
        1 tacggtagaa gcggcgatct atgaaggact ggatgctcct tcttaggggg ttgaatagtg
       61 cggcgatcaa gagggtagag gccacgactg cgagggtaga ctgctggccg gtgagagcga
      121 cgaagagccg ctgcagcaga acgatgccaa cgaagtagat caccactagc agcgccgtga
      181 gtgaaccgta gacgagagtg tggtttacga tgaagtctat gtcgtagagg cgatacttga
      241 ggatcgcgat acctgtcgcg acgggaagcc cgatgagggg aacctcatac aacagtgaag
      301 tgggcgatac gagccccacc agagtgaacg agaaccagat cacgaacaca acgatcatga
      361 cggccacggc gtacgcgaac catttcagtt gctggcgttc gtcacctctc gaccggcgga
      421 aacgtacgat caccgccgtc ccactcgcca cgattagcag cgggaacgcg tagttgaaga
      481 gctcgtagag caggcccatg atctcgaact ccaaccccaa ggggttacga acggctgcca
      541 gccgcaggtc gttggtctcc ggagatagcc aagacgccat cgtgaaaagc acgacggagc
      601 ccgcagtccc ccacaagacc ggacgccaac gtggagaggg cagcctcccc gtaggataca
      661 gcaagagtaa gaacgtaacg attacgaacc acccgattcc gcgcgcccac gcgccgaacc
      721 acgcagtcca cacccctgca ggcagcgcac ccgggtcggt gatcagcgag tagacgccgt
      781 attccagcgt gagctctccc actattcata cgaaggctcc agtgagaaag agccagccga
      841 ttgctttctc ggggcggtac gaggcgacca aagcgccgac cgtagagaag gtgaggatca
      901 ataccgggag gagtacctcg tcccacaggg agggtgaagg ctcgttgagt gaggcgaaaa
      961 ttagcgtgag gattgtagtc ggcacggtca acgcccacac actccacgca aaccaggcgc
     1021 cagcgcggtg gctcatccca tcgcctcttc gaccggttcg cttgcccctt ccaccacaac
     1081 cccactctac cgcactactg tctccgaagc gttgtgacga tcgctttctg ggctaaggag
     1141 agccgagatt ccgaaggtca tattccatgg aagccaggtg atatatgctt gcatccgcgg
     1201 aaccgggtgc ggattatcgc gggtgtgcaa gcatagaaaa gacgtttact cttctgtcag
     1261 gccaactccc caccgtccag gtagtgcggt cgcccgggtc ttatgtaccg acgtgggcgt
     1321 cgcgggcggc gccaatacct gcgatcaaca gacacatcgt aaccgcgagc agcaatgcca
     1381 gcggcacctt ccacgcaccc gtcacatcgc gcaggaggcc gaagaggaaa ggcccacccg
     1441 ctgcgagcag gtaaccgacc gattgggcca tacccgagag cgcagcggca tgcccggggt
     1501 ctggagcacg cagcgcgaag aaagtgagcg ccaggctgaa ggcagccccc tgaccgagac
     1561 caagcagcac gacccaaagg gtgctgacgg tggaaccggc aaccagcagg cccaaagcgc
     1621 cgacaccaga catgcccacg gctaccacaa cgacgcctcg ctgagagggt ctcctcccgg
     1681 caatcatagg ggtcaagaac atagtcacga tcgccactgc ctgagagagc ccgagcatcc
     1741 accccgccct ggccgcgctc atcccctccg cctgcaggat ctccggcagc cacgtcagcg
     1801 tcacgtagta ggagagcgat tgcaacccca tgaacagtgt cacctgccaa gccaggggcg
     1861 aacgccacag gtctctcacc ccaggcgtgc ttcgggcgga cgggtcccca ggatgatcgc
     1921 tacggctgag ctgggggagc caggcgacac cggcgaggag ggcgggcagg acccacacgg
     1981 ccaaggcacc tcgccagccg accagctgtg ccaaggggac gctcacgcca gcggcgagcg
     2041 cggcgctgat gcctagcgag ctggagtaca cgctcgtcat gaggccggcg tgttcgggga
     2101 actcccgctt gacgaggcta ggcaagagca cgttggctac cgcgatcgcc gctcccagga
     2161 cgaccgtacc caagaacaga gcctctacag cccccgccca gcgcaacagt atgccagcca
     2221 ccaggacgag cagactcgcc agcaacacgc gctccatgcc gaacaggcgg gctacgcgcg
     2281 gcgcgatcgg cgagagcagg ccgaaagcca ggagcggcaa agtcgtgaga agccctgcta
     2341 ccccgttgga tatgccggtg tccgcccgga tctggccgat caagggtgcc acgccggtga
     2401 gtgccgggcg caggttggcc gccagcagca cgatccccaa tacaaggaga acgctacgga
     2461 ggcgtgagcg gccggattct gccgtggcgg aaaccctctt cgtaatactg cgcctccctg
     2521 tcaccctgca gtggcaaggc gtagcctact cgacctggat gagaatggac cgggcgatcg
     2581 aagtccaaga gccgttcgca ggtcacaagt gcactgtacg aaagctagag tgtagatgct
     2641 agggattcgt tccgccgctc ccaaggggct gcggcggaat gaatctggac ggtcagccat
     2701 gatatgccga agccggagat cgagacccaa ggccaccccg cacttcgggg gcgaaggtgg
     2761 atggggctgg gttaacatgg caggatcatg agcgagcctg aggatctcgt ccggaccatc
     2821 accgaggagg gtgtgcgtga tccccgcctc ctcgaggcgc tgcgcgcggt ccccagggcg
     2881 ggcttcgttc ccgctaacct cgccggacaa gcctacgagg acaagccgct acccatcccc
     2941 cacgaccagg tgacgactca gccctcgctg gtggcgagga tggtggacgc tctggggctc
     3001 gctggctcgg agcgggtgct tgaggttggc gccggctacg cgtggcagac ggcgctgctc
     3061 gcccgcctgg gcggcttcgt gtgggcggtg gagcgcttcg gggacgtagc ggatgccgcg
     3121 agggagaacc tcgcccggtt cggcgtgacg aacgccgagg tcgtcacggt cgacggcacg
     3181 aagggtctac ccgagcacgc tccttacgac gccatcttgg tcgccgcagc gttcctttct
     3241 gtccctaggc cgctcgagga gcagctcgct gcggaagggc gcctggtaca gcctgtgggg
     3301 ccaggcggca gagaggaggt ggtcctcttc cagaagggca ctcgagggct agtgcggcgc
     3361 cgcactatcg ccttggctcg cttcgtcagg ctccatggcg cgcacgcatt cccggagcag
     3421 tagcgtttcg cgggcaacag ctggagaggt cgtcaccacg cggaaggagc gcggcgctag
     3481 ccgcgttcga acaccagggc gccttcgcga cgggcaaaga cggtgcccat ctcggcggac
     3541 aggcgttcca tccggtccag gatggtttcc ggctcgccgc ccgtagcgag gtcaacccgc
     3601 gcgtaagaga gcttgaccgg ggtaaggtca agacgctcga acctgccttt ttccatggaa
     3661 acctgaaaaa ggaaggacca gtcgttacgg aatttggggt caaccgcgta atcgtcgata
     3721 aaatcgcccg tatcgtagag gatgggcttg ccgcggtaga tctccacccc ttggaagacg
     3781 tgcgcactgt gcccgtagta gatgtctacc ccccgatcta tgacggcacg ggcgaaccgg
     3841 cggaagatct ctttgggccg ctgcaccatg ttggggcccc agtggttgga gaatatgatc
     3901 gtgcctaccc ccatttcgcg cacatacgac accgttctct ccacccgccg aagaacttcc
     3961 ggtttcaagg agaccggcag gtagttggtg ccaggccggt cgatgccggc cgcaaacggc
     4021 ggctcgttgt ccgtgaacgc cagcagcgcc acccggcgtg tgtggtccgc tggcacggta
     4081 aggatggccg gatctgcggc ttcttctcgg ttgcggccag ctccggcgtg ccgaatgccg
     4141 gcggcgtcca ggtgttccat ggtatcgagc aacccctgtt cctcgaagtc tagagtgtga
     4201 ttgttggcca gcgaacaggc atcgatccgg gccgtccgta aggtctcgat ggcagagggt
     4261 agggctctga agtggaagac tttgggcgta cgggtccacg gccgctcgtt atccgtgata
     4321 gcgcactcca ggttgatgat gcgtaggtcg gcagcgtcca gaaggggcat cacgtcaccc
     4381 cacggcgcct cgggacgcat gcggttgtca aggacctcgg cgaccccgcg cccgagcatg
     4441 acgtcgccgg tcagcgccag ggtaatcatc gcgccccagg tgccagatga cctgcccgaa
     4501 acgtgtcgca cgggtcgcat aaacatttca ctgtacggcc ttgtgtcaga cccggatact
     4561 ccggagtcat cttgctccac aagtgctcag ccacaatccc agaggtagtg tcaaacaact
     4621 cccgaccctg cgggtgtctt ctctcgtcgt cagcccgaag ctcgctgagg aaggtcattg
     4681 tcgatccacc atactgggaa cagtctacaa gagggtagca gcagatccca tgtgcagaac
     4741 actgtttcgt tccgccgttc ctagaggaag aaggtgcggc ggagactaat tggccttctc
     4801 gtagtatctc tcgaaggctt cttccgagaa cacgccccca gggtaagagt acccttcccc
     4861 ctccgcagtg acggcccgga tctcgtagtc tccagcttcg ttcttcctcg cccagtacag
     4921 ccccgaccat ccgcttgtcc tcatcttctc tgcattcttg tagcggtaga gctcgaagcc
     4981 ctcctcggga ggcaccaggt tcaggtcctt aaagcgttcc tggttcatgg ctccttagat
     5041 ctccctctct agcagcgccg gtaggatcag ccagtcctga tcgtcctcct ctcaagaggc
     5101 cgtttgcagc gctcggccct tgggctcaac tcctgatgat gagccggtag acggccagga
     5161 cgaccacggt tcccaggacc gctaccgcga agcttctgaa atcgaacccg aagtacaaca
     5221 cctccgtccc ggtcacgagc ctgtagaaga accctcctac gaaggcgccc gccactccga
     5281 gaagcatagt gattatgcac ccgccagggt cgcggcctgg catcagcagc ttcgcgatta
     5341 tccccgcgat caggccgaag actatccagc taagcacacc caattggcta ctcctccagg
     5401 tgcctgcgca ccttcggccg ctttgtggta ttgcaggtag attgtactag tatgctgttc
     5461 ctttgctgac ggaacgggtg gatggcatca agtcatttga tacccgtggt gggtataggt
     5521 agaatctcga aaagtggggt cgcgcggagg ctccccttcc gaattcgagg aggtatcgaa
     5581 gagatgacag agcagcaggt tgggcaggct cacggctaca tcaaagaggg tgacaaggag
     5641 aagctcaggg cgcgtttgag gcgcatcgag ggtcaggttc gcggggtgca gcgcatgatc
     5701 gatgaggaga agtattgcgt tgacatcctc acccagatct ccagctacat ccgcgcttcg
     5761 gagaaggtcg ccttgctcgt cttgagagat cacatggacc actgcgtgag ggaagcactc
     5821 gcggatgggg agaacgccca tgagaaagtc agggagctgc aggaggctat ggagcgcttc
     5881 ctgaagcttg actagcgcgt gctcgactgg gccgagatca agaaaccttc gggaaggcga
     5941 atatgagtgt aaaggacagg atggcggcag atccggtctg cgggatgaag gtggacatcg
     6001 tggtgggcct gcctggactc gaaccaggga
//
LOCUS       DASTKM010000235         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_955344, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000235 DASTKM010000000
VERSION     DASTKM010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955344"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VFQ10_09685"
     CDS             <1..771
                     /locus_tag="VFQ10_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET9965654.1"
                     /translation="VGTLASLGPAGTQVLGEFARELDLLEPVVPWHTDRVRISDIGCA
                     LSLVAGVLGKISQDIILMAQTEVGEVAEPAGEGRGGSSTLPHKRNPILSVTAAANARR
                     VLDLSHTLQAAMVSDHERAAGAWHSEWETLSDALAFGGGAAAAVREVTEGLEVYPEKM
                     LKNLNTTGGLLLAENVATVAAEHLGRLEAHELVEEASHRALESGKPLREEVLSEPRLG
                     EVLSEREIDEVLDPAHYLGSAGAFVDRALKLYREGEVK"
     gene            768..1556
                     /gene="pcaD"
                     /locus_tag="VFQ10_09690"
     CDS             768..1556
                     /gene="pcaD"
                     /locus_tag="VFQ10_09690"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HET9965655.1"
                     /translation="MSEEAVVELNYVLEGPEDAPVLVLSNSLGSTLGMWDDQAPVLRE
                     SFRLLRYDQRGHGDSPVPSGPYKIEDLGRDALALLDRLEIESASFCGLSIGGLVGMWL
                     GSEAPERVERLVLCCTAPRFDPEMYDVRASKVRAAGVGSIADVVLERWFTPEFRATRP
                     ETVESAGRMLRGTPAEGYAGCCEALRDADMRSRLGEIRAPTLVIAGAEDPAATVDQAE
                     EIRDSIPEARLVVIEAAHLANIEQPEAVTQEILEHLEPVVRDRS"
     gene            1553..1948
                     /gene="pcaC"
                     /locus_tag="VFQ10_09695"
     CDS             1553..1948
                     /gene="pcaC"
                     /locus_tag="VFQ10_09695"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HET9965656.1"
                     /translation="MTSEDSIYDRGMKVRREVLGDEHVEGAIGGTTEFTADFQDFITR
                     YAWGEIWSRPGLDRKTRSCITLTALVALGRLDELELHVRAAVRNGLTEREIGEVFLQS
                     AVYCGVPAANSAFAVARRVLKESERGKEE"
     gene            1949..3124
                     /locus_tag="VFQ10_09700"
     CDS             1949..3124
                     /locus_tag="VFQ10_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HET9965657.1"
                     /translation="MRTQVGIVGGGPAGLLLSHLLQLEGIESIVLERRSREDLETTVR
                     AGVLEHGTAELLKEVGVGERMLREGSVHHGIELRFGGRGHRIDFGDLTEGLSITIYGQ
                     QEVVKDLIRARLEADGKVLFEARAESVGDLDTDSPKIRFLQDGQQEEISCDYVAGCDG
                     SLGVCRTGIPENVRTQYTRTYPFGWFGILVQGPPSTEELIYTLHDRGFALVSTRSPEV
                     QRLYFQCDPQDDVEVWPDERIWDELTTRLATHDGWELAGGEIFQKDIVQMRSFVCEPM
                     QHGRLFLAGDAAHIVPPTGAKGMNLAVADIRVLAHALTEFYGSGDKELLDAYSEVCLR
                     RVWRAQRFSWWMTSMLHRFDRSDPFQLKVQQAELDYVTTSRAAATTIAENYVGAALG"
     gene            3177..>3869
                     /locus_tag="VFQ10_09705"
     CDS             3177..>3869
                     /locus_tag="VFQ10_09705"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET9965658.1"
                     /translation="MISGKTGLLALVGQPVGHSLSPAMHNAAFVAEGLDFVYVCLDVD
                     PDELPAAVRGMEALKLRGFNVTMPHKRAMIPLVDELDEEARISGAVNTVLIEDSGLRG
                     FNTDGGGMVMACKEAGIDLSGKSVLLLGAGGTAAAIAVAFGKAGVGELHIANRSVEHA
                     AYLRDKLHGTGMKGLVVHRLDTLPDAEIVINATPLGMKEGDPLPVPPAYVREGRGFCD
                     AVYRPGTETPLVR"
BASE COUNT          720 a         1160 c         1324 g          665 t
ORIGIN      
        1 gtggggacgc tggcctcgct aggaccggcg ggcacgcagg tcctcggcga gttcgcgcgg
       61 gagcttgacc tcttggagcc cgtagttccg tggcacacgg accgggtccg gatctcggat
      121 atcgggtgcg ccctctcgct cgtcgcgggc gtgcttggca agatctcaca ggacatcatc
      181 ctgatggccc agactgaggt aggggaggtc gccgagcccg cgggagaggg aaggggaggc
      241 tcttcgacgc ttccccacaa gcgaaacccc atcctctccg tgaccgccgc cgcgaacgcc
      301 agaagggtac tcgacctctc gcatacgctg caggctgcta tggtgagcga tcacgagcgg
      361 gccgcgggcg cgtggcactc cgagtgggag actctctcgg acgccctcgc cttcggtggt
      421 ggggccgccg ctgcggtcag ggaggtaacc gaaggattgg aagtctatcc ggagaagatg
      481 ctgaagaacc tgaacaccac cggaggattg ctgctcgcgg agaacgtcgc caccgtcgcg
      541 gcagagcacc tcgggcgttt agaggcgcac gagcttgtcg aggaggcctc gcatcgggcc
      601 ctcgaaagcg gaaagcctct acgcgaagaa gtattgtccg aacccagact cggcgaggtt
      661 ctctccgaga gagaaataga cgaggtgttg gatccggcac actacctcgg gtctgcagga
      721 gcgtttgtgg accgagctct gaaactatat cgtgaggggg aggtcaagtg agcgaagaag
      781 ccgtagtgga actgaattac gtcctcgaag ggccggagga cgcgccggtc ctcgtcttgt
      841 ccaactctct gggctccacg ctcgggatgt gggacgacca ggcgcctgtt ctccgcgaaa
      901 gttttcgcct tttgcgctac gaccaacgcg ggcatgggga ttcgccggtc ccttccggtc
      961 cctacaagat agaagatctc gggcgagacg cactggcgct gctggacagg ttggagatcg
     1021 aaagcgcttc attctgcggc ctctccatcg gcgggctcgt cggcatgtgg ctggggagcg
     1081 aagcgcccga gcgcgtcgag cgtctggtgc tgtgctgcac cgcgccccgg ttcgatccag
     1141 agatgtacga cgtccgcgcc agcaaggtca gggccgcggg tgtcggttct attgccgacg
     1201 tggtgctcga gcgctggttt acgcccgagt tccgcgctac ccgtcccgag accgtcgagt
     1261 cggcagggcg catgctgcgc ggcacgccgg ctgagggata tgcgggttgc tgcgaggctc
     1321 tcagggacgc cgacatgcgg agtaggttag gggagatccg ggctcccacg cttgtgatcg
     1381 cgggcgcgga agaccccgcg gccaccgtag atcaggcaga ggagatccgc gactccatcc
     1441 cggaggcgcg cctcgtcgtg atcgaggcgg cgcacctggc gaacatcgag cagcccgaag
     1501 ccgtgacaca ggagatcctg gaacatctcg agccggtcgt gagggacaga agttgacctc
     1561 ggaagactct atctacgatc gcggtatgaa ggttcgtcgc gaggtcttgg gcgacgagca
     1621 tgtggagggt gccatcgggg gcacgacgga gttcaccgcg gacttccagg acttcataac
     1681 caggtacgcc tggggcgaga tctggtctcg tcccggcctg gaccgcaaaa cgcgaagctg
     1741 catcaccctc accgcgctgg tcgcgctggg ccgcctcgac gagctggagc tgcacgtgcg
     1801 cgcagccgtc cgcaacggcc tgacggaaag agagatagga gaggttttcc tccagagcgc
     1861 cgtctactgc ggggtgcccg cggcaaactc cgccttcgct gtggctaggc gtgtcctcaa
     1921 agaatccgaa cgtggtaagg aggagtaggt gcgtacgcag gtagggatcg tcgggggtgg
     1981 ccctgcggga ctgctcctct cgcaccttct gcagctggag gggatcgaat ccatcgtgct
     2041 cgaacgtcgc agccgcgaag atctggagac gacggtcagg gccggggtgc tcgaacacgg
     2101 cacggccgaa ctgctcaagg aagtcggggt cggggagagg atgctaagag aaggctccgt
     2161 ccaccatggg atcgaacttc gcttcggcgg ccgcgggcac cgcatcgact tcggcgacct
     2221 gacagaaggg ctttcgatca ccatctacgg ccagcaggaa gtcgtcaagg atctgatccg
     2281 cgccaggctc gaagcggacg gaaaggtcct gttcgaggcc agggcagaaa gcgtaggaga
     2341 cctggacact gattcgccga agattcgctt cctccaggat ggccagcaag aggagatctc
     2401 ttgcgactac gtggccggct gcgacggctc tctcggggtc tgcaggaccg ggatcccgga
     2461 gaacgtgcgc acgcagtaca ccagaaccta tccctttggg tggttcggca tcctcgtcca
     2521 gggaccgcca tcgacggagg agttgatcta caccctccat gaccgcggct tcgccctcgt
     2581 cagtacccgc tctcccgagg tccagcgcct ctacttccag tgcgacccgc aagacgacgt
     2641 cgaagtgtgg cctgacgaga ggatctggga cgagctcacc acgaggctcg ccacccacga
     2701 cggctgggag ctggccgggg gcgagatctt ccagaaggac atcgtgcaga tgcgctcctt
     2761 cgtctgcgag ccgatgcagc atggcaggct cttcctggcc ggggacgccg cgcatatcgt
     2821 cccgccgaca ggtgctaagg ggatgaacct ggccgtcgca gatatccggg tcctcgccca
     2881 cgccctgacg gagttctatg gatctgggga taaagagcta ctcgatgcgt actcagaggt
     2941 ctgcctgcgc cgcgtttgga gggcgcagcg gttctcctgg tggatgacct ccatgttgca
     3001 ccgcttcgac aggagcgacc ctttccagct caaggtgcag caggccgagc tcgactacgt
     3061 cacgacttcg cgggccgcgg ccacgaccat cgccgagaac tacgtcggag ctgccctggg
     3121 ttgagcggca aggtcaacac ctcccaccct ctataatggg cggcgtgtcg agagggatga
     3181 tcagtggtaa gaccgggttg ctggccctcg tcgggcaacc tgtcggccac agcctgagtc
     3241 cggcgatgca caacgcagct ttcgtcgcgg aaggcctcga cttcgtctac gtctgcctgg
     3301 atgtggatcc cgacgaactg ccggcggcgg tgaggggcat ggaggccctg aagctcaggg
     3361 gcttcaacgt tacgatgccg cacaagcggg cgatgatccc gctcgtggac gagctggacg
     3421 aggaagcccg catatcgggc gccgtcaaca ccgtgttgat cgaagactcc ggattgcgcg
     3481 ggttcaacac ggacggaggc gggatggtga tggcctgcaa ggaggccggc atagacctct
     3541 cgggaaagag cgtcctgctc ctcggtgccg ggggtaccgc cgcagccatc gcggtcgcct
     3601 tcggtaaggc cggggtaggt gagttgcaca tcgccaaccg tagcgtggag cacgccgcgt
     3661 acctaaggga caagctgcac gggactggca tgaaaggtct agtagttcat cggctcgata
     3721 cgctccctga cgcggagatc gttatcaacg caacccctct agggatgaag gagggagacc
     3781 ctttgccggt accacccgct tacgtgcggg agggaagggg gttctgcgac gcagtctacc
     3841 gtccagggac cgagacaccg cttgtccgg
//
LOCUS       DASTKM010000236         4067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_602092, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000236 DASTKM010000000
VERSION     DASTKM010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602092"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFQ10_09710"
     CDS             complement(<1..387)
                     /locus_tag="VFQ10_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9965659.1"
                     /translation="MIEVAIMVEGQEGVGWDSWRRLALAVEDLGYAGLFRSDHFPNKR
                     TGMYRDGLELWSSLAWLAENTSRIEFGPLVSPVSFRHPVITAWQASSVDNLAGGRLRL
                     GLGAGWNGWEHETFGFDLLDIDRRFAR"
     gene            516..1400
                     /locus_tag="VFQ10_09715"
     CDS             516..1400
                     /locus_tag="VFQ10_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014261.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG/SRA domain-containing protein"
                     /protein_id="HET9965660.1"
                     /translation="MAERLFGHVPGYPEGSLFENRAELNASRVHTPTQAGISGSQTEG
                     AESIVLSGGYEDDADYDDTIIYTGHGGRDPSTGQQTHDQPFARGNRALALSKQNGLPV
                     RVIRGSSHDSPYSPPSGYSYDGLYAVEDFWHDVGKSGFKIWRFRLVKIPEETTPGQII
                     AEKPAEYSVPDRREVRVSRIIRDTVQARAIKALYDHRCQMCGTRLECPAGPYSETAHI
                     RPLGAPHNGPDTRDNILCLCPNHHVLFDNGAVSVADDLSLIGAESNLTVHRDHHINQH
                     YLAYHREHFVANRESYLS"
     gene            complement(1648..2031)
                     /locus_tag="VFQ10_09720"
     CDS             complement(1648..2031)
                     /locus_tag="VFQ10_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HET9965661.1"
                     /translation="MPDNVDEREVVARMAKKTDKVLKRLKKDVAKLREQNDNLAEALE
                     KTREDQAAANSDLRNLLEARLTAHDTAPGEQEDNSRDGGEGPKVTVAAQRRAKELGVD
                     LSSVKGTGSGGRVLVKDVEAATDGG"
     gene            complement(2055..2642)
                     /gene="leuD"
                     /locus_tag="VFQ10_09725"
     CDS             complement(2055..2642)
                     /gene="leuD"
                     /locus_tag="VFQ10_09725"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET9965662.1"
                     /translation="MEPVERITGTALPLGYSDVDTDQIVPSDALKRIERTGFGEFLFS
                     GWREDEDFIMHRAEHKDAVILIAGENFGSGSSREHAVWAIQDHGFGAVIAPSFADIFR
                     NNSTKNGLLAIDLPEETVENLLQAVRELPETVITIELDELTVTAPNVHETFEMDDFTR
                     RRIMEGLDDISLTLTHEDDLETFERSRPGYLPSVQ"
     gene            complement(2642..>4067)
                     /gene="leuC"
                     /locus_tag="VFQ10_09730"
     CDS             complement(2642..>4067)
                     /gene="leuC"
                     /locus_tag="VFQ10_09730"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET9965663.1"
                     /translation="RRMGRTLMDRPKTLVEKVWEKHVVRSAEGEPDLIYVDLHLVHEV
                     TSPQAFEGLRLAGRGVRRPDLTLATMDHNVPTSEFGLPIEDKVSAKQMEALRSNCEEF
                     GIKLNEWGSIGQGIVHIIGPETGLTQPALVIVCGDSHTATHGAFGALAFGIGTSEVEH
                     VLATQTLTQSRPKTMAVTVEGELPADVTAKDLMLGILNRIGTGGGVGHVIEYRGEAVR
                     QLSMEGRMTVCNMSIEGGARAGLVAPDETTFEYLEGREYAPKGEHWEAAVEEWKKLPT
                     DEGAEFDKEVVIQAEDLVPYVSWGTTPAQTVPLDGEVPEPQNEGHQRALKYMALQPGT
                     LIRDIEVDTVFLGSCTNARIEDLRAAAGVLEGHKVKEGIRAMVVPGSMRVKKQAEEEG
                     LADIFTEAGFDWRNAGCSMCLGMNPDILQPGERCASTSNRNFEGRQGKGGRTHLVSPV
                     VAAATAVMGRFASPSELSVPMEVS"
BASE COUNT          742 a         1287 c         1181 g          857 t
ORIGIN      
        1 gcgggcgaag cgccggtcta tgtcgagcaa gtcgaagccg aaggtctcgt gctcccatcc
       61 gttccagccc gcgccaagtc cgagcctgag gcgtccgccg gccaggttgt ccacggagga
      121 ggcctgccag gcggtaatga ccggatgccg gaatgagacg ggcgagacca gaggcccgaa
      181 ctctatgcgg ctggtattct ccgcaagcca ggcgagcgag gaccacagct ccagcccatc
      241 cctgtacata cccgtccgct tgttggggaa gtggtctgag cgaaacagtc cagcgtagcc
      301 aaggtcctcg accgccagcg ccagccgtcg ccagctgtcc caaccaacac cctcctgccc
      361 ctcgaccatg atcgcgactt cgatcatcta ttctcccctc agataagccg accgcattaa
      421 ttctagcctg tggcgaggct gccgaataaa atgctcacta tgactatgtt tggattcgca
      481 aatctttgcg aacagctgat ccggtaggca actgtatggc ggaacgactt ttcggacacg
      541 tgcctggtta cccagaagga agtctcttcg aaaatcgcgc tgaactgaat gcgagtaggg
      601 tccacacacc tactcaggcg ggtatctctg gaagtcagac agagggagcg gaatcaatag
      661 tcctctccgg tggctacgaa gacgatgcag actacgatga tacgatcatc tacaccgggc
      721 acggagggag agatccaagt actggacagc agacccatga tcaacccttc gcccgcggca
      781 atcgggctct cgcgcttagt aagcagaatg gccttccagt ccgcgttatt cgaggttcca
      841 gccacgactc gccatattca ccaccctccg gctatagcta cgatgggttg tacgcggtcg
      901 aagacttctg gcatgacgtt ggtaagtcgg ggttcaaaat ctggcgcttc cgcctagtaa
      961 agattccaga agagacgact cctggtcaga taatcgctga gaaaccagcc gagtactcgg
     1021 ttccggaccg cagagaagtt agagtttcgc ggatcattag agatacggtg caggcaagag
     1081 caatcaaagc cctttacgat cacagatgcc agatgtgcgg aacccgtctg gaatgtcccg
     1141 caggtcccta ctcagaaacg gcgcacatac gcccgctcgg ggctccgcac aacggtccgg
     1201 atacaagaga caacatcctc tgcctgtgtc ctaatcatca tgtgctcttc gacaacggcg
     1261 ctgtatcggt cgcagacgat ctatctttga tcggcgctga gagtaacctc accgtccaca
     1321 gagaccacca catcaatcag cactatctcg cctaccatcg ggagcatttc gtcgcgaacc
     1381 gcgaaagtta tctttcttga acacgagtgg ttgaatgcgc ggagcgcctg cagcagtgga
     1441 ttcccgcttt cgcgggaatg acggtaatgc tggaagggta ggacttatgc tcgccacgtt
     1501 gtacaccgcc gaagtgagca tgcgggttcc gtatgatcag ccttacagat tcgtccagag
     1561 ggcggcaatt agttcggtag caccttctcg aacgtccgca acctcacgtc atcgagacct
     1621 tagcgtattt ggaggtcggg atgcttatca tcccccgtcg gtggcggctt ccacgtcctt
     1681 gacgaggacg cggcctcctg agcctgtacc cttcacgcta gagaggtcta cgccaagctc
     1741 cttggcccgc cgctgcgccg ccaccgtgac cttcggtccc tctccgccgt cgcgcgagtt
     1801 gtcttcctgc tcgcctgggg cagtgtcatg cgcggtcagc cgcgcttcga ggaggttgcg
     1861 gagatcgctg ttggctgcgg cctgatcctc gcgcgtcttc tcgagcgctt cggcgaggtt
     1921 gtcgttctgt tcccttagct tggccacgtc cttcttcagg cgctttagaa ccttgtccgt
     1981 cttcttggcc atcctcgcta ccacctctcg ttcgtcgacg ttatccggca atattgtacg
     2041 ggccttatcc cgggctactg cacgctcggc aaataacccg gccgtgaccg ctcaaaggtt
     2101 tcgagatcgt cttcgtgcgt tagcgtgagg ctaatgtcgt cgaggccttc catgatgcgt
     2161 ctgcgggtga agtcgtccat ctcgaaggtt tcgtggacgt tgggggcggt gacggtgagc
     2221 tcgtcgagtt cgatggtgat cacggtctcg ggaagttccc tgacggcttg aagaaggttc
     2281 tcgacggtct cctctggcag gtcgatggcg aggagaccgt tcttggtcga gttgttcctg
     2341 aagatgtcag cgaaagacgg ggctatcacc gcgccgaaac cgtgatcctg aatcgcccag
     2401 accgcatgct cgcggctgga accggagccg aagttctcgc ccgcgataag tatcacggca
     2461 tctttgtgct cggccctgtg cattatgaag tcctcgtcct cgcgccagcc ggagaacagg
     2521 aactctccga agccggtgcg ctcgatgcgc ttgagggcat cgctagggac gatctggtcc
     2581 gtgtccacgt cggagtaacc gagcggaagg gctgttccag tgatgcgctc gacaggctcc
     2641 actagctcac ctccattggt acgctgagct ccgagggcga ggcgaacctg cccatcacgg
     2701 ccgtcgccgc ggcaaccacg gggctgacca ggtgtgttct gccacccttt ccctggcgcc
     2761 cctcgaagtt gcggttcgag gtcgaggcac accgctcgcc tggctgaagt atgtcggggt
     2821 tcatgccgag gcacatcgag caccccgcgt tgcgccagtc gaagccggcc tccgtgaaga
     2881 tgtcagcgag tccttcttct tccgcttgct tctttacgcg catagaacca gggacgacca
     2941 tcgcccttat gccttccttg accttgtggc cctccaggac gccggcggcg gcgcgcaggt
     3001 cctcgatgcg ggcgttggtg caagacccga gaaataccgt gtctacttct atgtcccgga
     3061 tgagggtgcc cggttggagc gccatgtact tgagcgccct ctggtggccc tcgttctgcg
     3121 gctcggggac ctcgccgtcg agcggaacgg tctgcgccgg ggtcgtgccc caggagacgt
     3181 agggaactag gtcctccgcc tggatcacga cctccttgtc gaactcggcg ccctcgtcgg
     3241 tcgggagctt cttccactcc tcgaccgccg cttcccaatg ttcgcccttc ggggcgtact
     3301 ccctgccttc caggtactcg aaggtcgtct cgtccggcgc tacgaggcct gccctggccc
     3361 cgccctcgat ggacatgttg cacacggtca tgcgtccctc catcgagagc tgcctgacgg
     3421 cctcacccct gtactctatg acgtgcccga ccccgccgcc cgtcccgatg cggttcagga
     3481 tgccgagcat gaggtccttg gccgtgacgt ccgccggaag ctcgccctcg acggtgacgg
     3541 ccatggtctt cggcctgctc tgggtgagcg tctgggtggc gaggacgtgc tcgacctcgc
     3601 tcgtcccgat gccgaaagcg agcgcgccga acgcgccgtg ggtggctgtg tgggagtcgc
     3661 cgcagacgat cacgagcgca ggctgagtga gccccgtctc gggcccgata atgtgcacga
     3721 tgccctgccc gatggagccc cactcgttga gcttgatgcc aaactcctcg cagttgcttc
     3781 tcagggcctc catctgcttt gccgaaacct tgtcttctat gggcaagccg aactccgagg
     3841 tcggaacgtt atggtccatc gtcgccaggg tcaggtcggg acggcgcact cccctcccgg
     3901 cgagcctgag gccctcgaaa gcctgagggc tcgtcacctc gtgaaccagg tgcagatcga
     3961 cgtagataag atcgggctcg ccctcggcgc tccgtaccac atgcttctcc cacaccttct
     4021 ctaccaacgt cttcggccta tccatcagcg tccttcccat tctccga
//
LOCUS       DASTKM010000237         8747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_603395, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000237 DASTKM010000000
VERSION     DASTKM010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603395"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFQ10_09735"
     CDS             <1..722
                     /locus_tag="VFQ10_09735"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9965664.1"
                     /translation="CLPVPWFGASLSLAGLAWSWPVLFLPALGCSFVFFLTGLRVVHN
                     AHHYALGLRRWTTEWVMFVMSVLMLGSMHAVQRNHLQHHKHCMDDEDLEGYSARLSAW
                     RALLYGPVFLVRLHMRGFRLGRRRHRRWILAELAAGALWVFLVFALSGSFALWYHVAV
                     MAVGQSMTSFFAVWTVHHDCDRSHQIARTLRGKVKNRISFEMFFHLEHHLFPKVPTCH
                     LPRLADRLDSAAPELQAHQVF"
     gene            931..1884
                     /locus_tag="VFQ10_09740"
     CDS             931..1884
                     /locus_tag="VFQ10_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9965665.1"
                     /translation="MDHNDFAERVERIVGNVELAVSGKRDSIFLAVVAMLAGGHVLIE
                     DVPGVGKTLLAKSLARSLRAEFRRIQFTPDLLPADVTGLNVYNQGEGDFEFWAGPVFA
                     NVVLADEINRASPKTQSALLEAMEEGSVTVDGVTRHLSQPFCVIATQNPVEHEGTYPL
                     PHAELDRFMIKMHVGYPDEDAETEMLKLSQDPVAELSYVVGVEDFLQMRRLVEEIYTS
                     EAVLRYVVSVTSATREHEDVYLGASPRASRMLLAASRARAAANGRSYCAPDDVKAMAS
                     SVLSHRIIPSATSSEDGLGPDYTTREIVRDILRSVPVTEAV"
     gene            1891..3138
                     /locus_tag="VFQ10_09745"
     CDS             1891..3138
                     /locus_tag="VFQ10_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9965666.1"
                     /translation="MPGRGASRLSVRPTARGWQALLAGAVTLVVARLIGTTQFHQLAY
                     ALLALPLASLFLGVIGSRGLRYSRALPPGVRITAGSPAKLRLLLERSRFGAPKASVVD
                     RLPGPRGFDFSSTRERSRIEVPITFARRGVYTLGPAEVRVADPFGLLRFARMDWETTE
                     VVVYPRVYELRGFPLRGGNVEAGTTGSRGRRGEEFANLREYRRGDDRRHIHWKSLART
                     GELFVKEFSLQAPRRHTVALDLRREGLRTQENEIEDAVSAAASALTHLAGEGLPFRLL
                     DTGSSGGDTGFGSDEDSYWEAMRHLATARADGVSHLGETVLGERGGLGEGVILVLRTR
                     DDELPHCVRKLREQGLSVIVIALATHTYRTPPVPGSAAQSREAEFLRSVGTLEAAGAT
                     VRVLSHPAGVAGLSGARRAEAVR"
     gene            3135..5387
                     /locus_tag="VFQ10_09750"
     CDS             3135..5387
                     /locus_tag="VFQ10_09750"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HET9965667.1"
                     /translation="MSGLGTRLWLYGAALATGSAFSILFTGETYDGADGGFGALSVVT
                     VIAGHKALLPLLIAAAVAALVGSAGRWRFVLLLPATAAYTLVAVYGTDLFSVSGWQGF
                     FRVIWGDVYGAANTMYVQPIPYDLAPGLFVVLVPLVMILVAFATSATLYEESPVISVA
                     VLGLTIGVLSTISFEDGAGPFFAVFLVCAVGLFLSAGGAGSDGPGRPAVVGGAIVIAL
                     VLLVPRMPFSDETVSTGWIDWTRIGTGGTSRLGVQADVGDYLTAGREAELLRIRSSEP
                     LLWRGGTMDYFDGVRWSDTTQAGADDGEEIARDVETRPVLQRVQVLNAQTDLIFGGYK
                     IVRTSEYLATPNSDGSWSMDEPFEEGDPYEVLSEIPQPTAAQLRGAGSDYPSTVEEKF
                     LQLPEDTPPVVSETANKIQRRYEPATPYDRARAIEQYLIYDGGFTYNLDVSYRRADKA
                     VEEFLGEGKEGFCTQFSTSMALLLRDMNVPSRVVYGATSGEEVSEGEYLVRGSNMHTW
                     VEAYFPGVGWYPFNPTPGFSMPDAMEANARRPELPLASGVQAAENSPDRSQLVQNRAQ
                     QQSREQNQDLANQDSTTGRSRESNIPAWPLFVLAPSLIVAAVPVSKRALLARGRPEDL
                     YMDLTGRLRDVLPPNGSIVADSPALTPTERILLLAGAAGVEETPMRRFALAYSDHLYS
                     ASDRRDHVVSAYRDALRAYEGLPRWRRALGAVNPSSLVARSRKYISAQKAQLGKVLRG
                     RLRRNLRKRR"
     gene            5452..5796
                     /gene="erpA"
                     /locus_tag="VFQ10_09755"
     CDS             5452..5796
                     /gene="erpA"
                     /locus_tag="VFQ10_09755"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET9965668.1"
                     /translation="MEQKEATIVNITDNAAEKVKTLMAQEGEEDLALRIGVRPGGCSG
                     FQYSIYFDDEIADDDEVLETKGVRVLVDAMSVPYIMGSEFDYVDGLMGAGFAVNNPNV
                     QGGCGCGSSFTC"
     gene            complement(5935..6489)
                     /locus_tag="VFQ10_09760"
     CDS             complement(5935..6489)
                     /locus_tag="VFQ10_09760"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9965669.1"
                     /translation="MAKGEGWKRLGSERLMETPYFSLRSDRLRLPDGGIKDPYYVIER
                     PDAAIIFPLTLDGQVVLVSQYRPPLDMTELGLPAGLVEDGERPEEAARRELSEETGYT
                     GGEWELLGSVASSPSLKDNRAYLFLARGVEETMSPDPDEHELVEVVRVPVKELLALVR
                     SGKIVSSSGVAAIMLALERLREDP"
     gene            7240..7665
                     /locus_tag="VFQ10_09765"
     CDS             7240..7665
                     /locus_tag="VFQ10_09765"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET9965670.1"
                     /translation="MRRSLVLVLVVALAALVFAPAAFTQDDNPSADDLRGDDRVGARG
                     FDDNPSRDDLGPDDNSADSEERLTGLDAQKAKSAAIAAVGGGTLTEVERDDGDDGYGT
                     SGVYEVEVKREDGTQVEVHLDGDYDVVGQQADDDGPNDD"
     gene            complement(7799..8062)
                     /locus_tag="VFQ10_09770"
     CDS             complement(7799..8062)
                     /locus_tag="VFQ10_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965671.1"
                     /translation="MSERPSFWQRLFGRTGSSSLSPRQQKVSDYIIGRMENEVPLQQV
                     LREDYVRRNCSQAEVEQIVSSPEFIETARERLGESFGSEEFKL"
     gene            8387..8701
                     /locus_tag="VFQ10_09775"
     CDS             8387..8701
                     /locus_tag="VFQ10_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965672.1"
                     /translation="MTDREAAQSFEAAGWAIREKHAPREEGVVGQAGRYAIRTTEKIL
                     GTETVYELHDEELNMKVYARKVPSPGRAAKLIEEYGAPAEISDVTPGKVPMVPPETEE
                     DR"
BASE COUNT         1618 a         2671 c         2829 g         1629 t
ORIGIN      
        1 tgtgtctccc ggtgccgtgg tttggagcgt cgctgtcact tgcggggttg gcgtggtcat
       61 ggccggtgct gttcttgccc gctctgggat gttccttcgt attcttcttg accgggttgc
      121 gggtggtaca caacgcgcac cattacgctc ttgggctacg gcggtggacg accgagtggg
      181 ttatgttcgt gatgagcgtg ctcatgctcg ggtccatgca cgccgtgcag cgcaaccacc
      241 tgcagcacca caagcattgc atggacgatg aagacctgga gggctatagc gcgcgcctca
      301 gcgcctggcg ggctttgctc tacgggccgg tcttcctggt tcggctgcac atgcggggct
      361 tccggctcgg gcggcgtcgg caccggcgct ggatcctggc ggagctagcc gcaggcgccc
      421 tctgggtgtt cttggtcttc gcgctctccg gcagcttcgc gctctggtac cacgtggcgg
      481 tcatggcggt gggccagagc atgacctcct tcttcgccgt gtggaccgtg caccacgatt
      541 gcgaccgctc gcaccagatc gcacgcaccc tgcgcgggaa ggtgaaaaac aggatctcct
      601 tcgagatgtt cttccacctc gagcatcacc tcttcccgaa agtgccgacc tgtcacttgc
      661 ccaggctcgc cgaccggctc gacagtgccg ctcctgaact gcaagcccat caggtcttct
      721 gaacccgagc cagtaagccg tcgtgcttca tcagcggtca gccttttggt cggcggtcgc
      781 cctatgcctg gcattgtgga tggccaaaga ggcgtccgcg acaagagagg ctcgcgtcac
      841 tcggggagaa gggtcgaaag tctctagctg agagctggtg actataagct ataatgccct
      901 ctgtcacccg agtacataga caggacttct ttggaccaca acgatttcgc ggagagggta
      961 gaacgcatag tcgggaacgt cgagcttgct gtttctggca aacgcgatag tatcttcctg
     1021 gcagtggtag ccatgcttgc gggcggacac gtcctcatag aggacgtccc cggtgtaggc
     1081 aagacgcttc tggccaagtc gctcgcccgt tcgctaaggg ccgagtttcg caggatacag
     1141 ttcacccctg accttctgcc ggccgacgtg accggcctca acgtctacaa ccagggcgag
     1201 ggagacttcg agttctgggc cggacccgtc ttcgcaaacg tggtgctcgc ggatgagatc
     1261 aaccgcgcca gtcccaagac ccagagcgcg ctgctcgagg cgatggagga gggttcagtg
     1321 acggtggacg gggtgactcg acacctctcc cagcccttct gcgtgatcgc cacccagaac
     1381 cccgtcgagc acgaaggcac ctacccgctg ccgcacgccg agctcgatcg gttcatgatc
     1441 aagatgcatg tcggctaccc cgatgaggac gccgagaccg agatgctcaa gctctcccag
     1501 gatcccgtcg ccgagctctc ttacgtggtc ggggttgagg acttcctcca gatgcgccga
     1561 ctggtcgagg agatctacac cagcgaggcc gtactcaggt acgtcgtctc cgtgacctct
     1621 gcgacccggg agcacgagga cgtctatctg ggcgcctccc cccgcgcgag caggatgctg
     1681 ctggccgcct ccagggcgcg tgcggcggcg aacggacgct cgtactgcgc gcccgacgat
     1741 gtcaaggcta tggcctccag cgtgctctcg cacaggatca taccctcggc caccagctcc
     1801 gaggacggcc tcggccctga ctacaccacc cgtgagatcg tgcgggacat cctgcgctct
     1861 gtcccagtga ccgaggcggt gtaagggccg ttgcctggga ggggcgcctc gcggctctcc
     1921 gtcaggccta cggcccgcgg ctggcaggcg ctcttggccg gggccgtcac gctggtcgtg
     1981 gcgcgtctga tcggtactac ccagttccac cagcttgctt acgcgctcct ggctctgccg
     2041 ctcgcatccc tcttcctcgg ggtgattggt tcaagaggcc tccgttactc gcgcgccctg
     2101 ccccctggtg tccgtatcac ggcgggcagc cccgcgaagt tgcggctcct gctagagcgc
     2161 tcgcggttcg gagcgccgaa ggcgagcgtg gttgaccgtc tccccgggcc gcgcggcttc
     2221 gatttttctt cgacgaggga gaggagcagg atcgaagtcc ccatcacgtt cgcgcgcagg
     2281 ggcgtctaca cgctgggccc agcggaggtc agggtggcag accccttcgg gttactccgg
     2341 ttcgccagaa tggactggga gacgaccgag gtggtcgtct acccgcgggt ctacgaactt
     2401 cggggctttc ccttgcgggg cgggaacgtg gaggcaggca cgacagggtc ccgtggccgg
     2461 cggggcgaag agttcgcgaa cctcagggag taccgcaggg gcgacgacag gcgtcacatc
     2521 cactggaaga gtctggcacg cactggggag cttttcgtca aggagttctc cctgcaggcc
     2581 ccgaggcgcc acaccgtggc cctggatctg cgcagggagg ggcttaggac ccaggagaat
     2641 gagatcgagg acgccgtctc ggcagcagcc tcggcgctca cacaccttgc cggcgagggt
     2701 ctacctttca ggctgctcga cacgggcagt agtggcgggg acacgggttt tggatccgac
     2761 gaagattcct actgggaggc gatgaggcac ctcgcaacgg cgcgcgccga cggggtctct
     2821 cacctgggag agaccgtgct cggagagcgg gggggactcg gggaaggcgt aatcctcgtc
     2881 ttacgcaccc gcgacgacga gttgcctcat tgcgtacgca agctcaggga gcagggcctc
     2941 tcggtgatcg tgatcgccct cgcgacccac acctacagga cgcctcccgt gccggggagc
     3001 gccgcacaga gccgcgaggc ggagttcctc aggagtgtgg gcacgctgga agctgcaggt
     3061 gccacggtgc gcgtccttag tcatccggca ggagtggcag gactctcggg tgccaggagg
     3121 gccgaggcgg tgcgatgagt gggttaggga cgagattgtg gctctacggc gcggcgctcg
     3181 cgacgggcag cgccttcagc atcctgttca cgggcgagac ttacgacggt gccgatgggg
     3241 gtttcggcgc gctctccgtg gtcacggtca tagccgggca caaggctttg ctgcccctgc
     3301 tcatcgccgc ggccgtggcg gcgctggtcg gctcggcggg caggtggcgg ttcgtgcttc
     3361 tcctccctgc cacagccgcc tatacgttgg tcgcggtgta cggtaccgat ctcttctcgg
     3421 tctcgggctg gcaggggttc ttcagggtca tttggggcga tgtctacggc gcggcgaaca
     3481 ccatgtacgt ccagcccata ccctacgatc tcgccccagg gctcttcgtc gtgctcgtgc
     3541 ccctggttat gatcctggtg gccttcgcaa cctcggccac cctctacgag gagagccccg
     3601 tcatctccgt ggccgttctg gggctcacca taggcgtact ctcgaccatc agcttcgagg
     3661 atggggcagg acccttcttc gccgttttcc tggtctgcgc cgtcggactc tttctctccg
     3721 caggcggggc gggatccgat ggtcctggcc gccccgcggt cgtgggtggg gccatagtga
     3781 tcgcgctggt gctcctcgtg ccgaggatgc ccttctccga cgagacggtc agcacaggat
     3841 ggatagattg gacgcgcata gggacgggtg gcacctccag gctcggcgta caggccgatg
     3901 tcggagatta cctcacggcc gggagggagg ctgagctcct gcggatccga agctccgaac
     3961 cgctcctgtg gcgcggcggg accatggatt acttcgacgg cgtccggtgg tcagacacca
     4021 cacaggcggg ggccgacgac ggggaggaga tagcccgcga cgtcgagacc cgccccgtgc
     4081 tgcagagggt gcaggtcctc aacgcccaga cggacctcat cttcgggggg tacaagatcg
     4141 tgcgtacctc cgagtatcta gcgaccccga actccgacgg ctcgtggtcg atggacgagc
     4201 ccttcgaaga gggcgacccc tacgaggtgc tctcggagat cccgcagcca acagcggcac
     4261 agctcagggg cgcgggcagt gattacccgt cgactgtgga ggagaagttc ctccagctac
     4321 ccgaagacac gccgcccgtg gtcagcgaga cggcgaacaa gatccagcgc cgatacgaac
     4381 ccgcgacgcc atacgataga gcgcgcgcga tcgagcagta cctgatctac gacggcggct
     4441 ttacctacaa cctcgacgtg agctaccgaa gggcggataa ggcggtggag gaattcctgg
     4501 gtgaaggcaa ggaaggcttc tgtacccagt tctcgacctc gatggcgctc ctcctgcgcg
     4561 acatgaacgt gccctcgcgg gtcgtctacg gggccacctc gggtgaagag gttagcgagg
     4621 gagagtacct ggtaagaggt agcaacatgc acacctgggt agaagcctac ttccccggcg
     4681 tcggctggta ccccttcaac ccgacacctg gcttctccat gcccgacgca atggaggcca
     4741 acgccaggag gcctgagctc ccgctggcca gcggggtcca ggccgccgag aacagcccgg
     4801 acaggagcca gctcgtgcaa aacagggcgc agcagcagtc cagagagcag aaccaggacc
     4861 ttgccaatca ggattcaacc acgggccgct ccagggagag caatattcct gcgtggcctc
     4921 tgttcgtgct cgcgccctct ctgatcgtgg cggccgtgcc agtctccaag agggcgctcc
     4981 tcgcccgcgg caggcccgag gacctctaca tggacctcac aggcaggctg cgcgacgtgc
     5041 tgccgccgaa cgggagcatc gtggccgact cgccggcgct gacccccacc gagcgaatac
     5101 ttctcctcgc cggagccgca ggcgtggagg agaccccgat gcggaggttc gccctggcct
     5161 actcggacca cctctattcc gcgagcgata gaagagacca cgtagtctcc gcttacagag
     5221 acgccttgcg ggcctacgag ggcctaccgc gctggaggag ggcgctcggg gccgtgaacc
     5281 catcctcgct ggtagcccgg tcgaggaagt acatctcagc ccagaaggcg cagctcggta
     5341 aggtgctgcg tggaaggctt cgccggaacc tacgaaagcg gcgttgaagt atacggttgg
     5401 cggtgtaata atttccgtac tcgatagcag actacagcac tggaggtgct gatggaacag
     5461 aaggaagcga ccatcgtcaa catcaccgac aatgccgccg agaaggtcaa gaccctcatg
     5521 gcccaggaag gcgaggaaga cctcgcgctg cgtatcggcg tcaggcccgg gggctgctcc
     5581 ggtttccagt acagcatcta cttcgacgac gagatcgccg acgacgacga ggtcctcgag
     5641 accaagggcg ttagagtgct ggtcgacgcc atgagcgtcc catatatcat gggctcggag
     5701 ttcgactatg tggacggcct catgggggcc ggtttcgccg tcaacaaccc caacgttcag
     5761 ggcggctgcg gatgcggcag ctcctttacc tgctagaccg ctgactcatt tagtcagtaa
     5821 cgagcccgga gcatagagtt ccgggctttt tctttgcccc gatatcaacg gcaacgcttg
     5881 gccatgtaga tgtcaggttt gccccatccg ttgccgtctg gcatcgcgcc ggcctcaagg
     5941 atcttcccgc agccgctcca gggcgagcat gatggctgca accccactgg aactgactat
     6001 cttgcctgag cggacgaggg caagaagctc tttgaccggc acccgaacca cctccacgag
     6061 ttcgtgctcg tcggggtcag gcgacatcgt ctcctcgacg ccgcgtgcca ggaagaggta
     6121 ggcccggttg tccttgagac tgggggagga ggccacggag cccaatagtt cccactcgcc
     6181 acccgtgtag cccgtctcct cagagagctc gcggcgggcc gcttcctccg gtctctcgcc
     6241 atcttcgact agtcctgccg gcagaccgag ctccgtcatg tcgagcggcg gacggtactg
     6301 gctgacgagg acgacctggc cgtctagagt aagcgggaag attatcgctg cgtcggggcg
     6361 ttcgatgacg tagtaggggt ctttgatccc gccatcgggc aaccgcagcc ggtccgagcg
     6421 taacgagaag taaggggtct ccatgagcct ctcgctaccc agtcgcttcc acccttcgcc
     6481 cttagccaac gccgccctcg tcgatggtgt ctgggatgcg gccggcgagt atccgcgaaa
     6541 ggtccttcgt caaagactta cacactggga aacaagatca tcattaggag cagtgcggac
     6601 cacagggaga taacgttggt gtagctgtca atggtcaccc ttctctccag atacgttaac
     6661 aacctgcgta aggatagacg aacagagaga tcagccgcaa ggcaaacgtt gcccccaccg
     6721 cagcgagctc tagctggcat tcctaagaga tcactagaag ccatctcgta aatagagagg
     6781 gagcagattg aacccactcg gcaagcactg cgggtagcgt cttctcgcct tttctagggt
     6841 gtgatgctct ctgcagatgc gtctttacca gcggtccggg tatttatgag atgagttcta
     6901 gtgatatgca ccccaccaga ggcacgagga tccatcctaa gggactgcaa tcacctctgg
     6961 agggtagggg accccacaga gacacagggc gaataaaggc tccatttgca ggtacgatat
     7021 gctctacttc cttcttaaag cctctcctct tcgagcacga gagaggccca tacacccgag
     7081 tgcgtagaac ttcgcctgtt gggaattcta ggaaatccgc atagccccga cccttcctac
     7141 tctgttgcaa tacgttggcc atcctgtaat ccctgtgtta cctagcatcg ttaagttcgg
     7201 ctacaggcat gcaaacggag cgcaagagaa aggaggggta tgcgtagatc cttagtgcta
     7261 gtgctggtgg tagccctcgc cgcgctggtc ttcgcgccgg cagccttcac ccaggacgac
     7321 aaccctagcg cggatgacct tagaggcgac gatcgcgtag gcgcacgagg cttcgacgat
     7381 aatccaagta gggacgatct cggacctgac gataacagcg ctgactccga ggagcggctc
     7441 accggcctcg acgcccagaa ggcgaagagc gccgcgatcg ctgcggtggg cggcggaacc
     7501 ctcaccgaag tcgagcgaga cgatggagac gatgggtacg gcaccagcgg cgtctacgag
     7561 gtcgaggtca agcgtgagga cggcacccag gtcgaggtgc atctcgatgg cgactacgac
     7621 gtcgtcggcc agcaagccga cgatgacgga cccaacgacg actgagatcc caccagcgga
     7681 tcctcaatta ctactatcgt agctggcgga agggccgggg tcagaggctc cggtccttct
     7741 tcttgccctt attcaccgaa gtgcctagaa ggtgaatact aagaagttgg tcgcactctt
     7801 agagtttgaa ctcctccgat ccgaaagact ctccgaggcg ttccctggcg gtctcgatga
     7861 actcaggaga gcttacgatc tgttcgacct cggcttggga gcagttgcgc cttacgtagt
     7921 cttctctgag tacctgctgc aggggtacct cgttctccat gcggcctatg atgtaatcgg
     7981 agaccttctg ctgacgaggg gagagggagg agcttcccgt gcggccgaag agccgctgcc
     8041 agaaggatgg ccgttcgctc atgaggatcc gtgagtcctt ccattagctt atgcattgtg
     8101 ggcttgatct tacccgctcg tgagctcgag gtatatccaa gaggactatt cggccgagtg
     8161 cgcggaaggc ccttctgcga ggtctgactt ccgctttacg gaattctagg aagttcgccc
     8221 ttgagatggt gccctaggtt tagtcctgtg gcaacataac ctgggtcggt gcactagtgg
     8281 tacggtgacg ggcagccgag tgcgggccac cggtccggcc ttttgtggca cagggatgcg
     8341 aggtgagtcc gcgcaagcga ggtcaatgaa atcgaggagg cacctcatga ccgacagaga
     8401 ggcagcgcag agcttcgagg cggccggctg ggcgatacgg gagaagcatg ctcccaggga
     8461 ggaaggggtt gtcgggcaag ccggcaggta cgctatccgg accaccgaga agatcttggg
     8521 cacagaaacg gtgtacgagc tccacgacga agagctgaac atgaaggtct acgcgcgcaa
     8581 ggtcccctcc ccggggcggg cggcgaagtt gatcgaggag tacggcgcgc cggcggagat
     8641 ttcggacgtg accccgggca aggtcccgat ggtgcccccg gagacggagg aggatcggta
     8701 gcgtcgacta cggggtgtgt ggaagcgatg ctagttaagg gaatctc
//
LOCUS       DASTKM010000238         4513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1311591, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000238 DASTKM010000000
VERSION     DASTKM010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311591"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VFQ10_09780"
     CDS             <1..831
                     /locus_tag="VFQ10_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9965673.1"
                     /translation="RPHLVVLQGPSLGDFFEITDDELVLGSDPFSADIVVRDVEVEPR
                     HAELYKRPGGYALRGVEAGKTIALNGEVMEEPGCGQLLSDGDRIHVGDSVLEFSHQDE
                     IKASFFEQLHHLVNRDHLTGLLSKGRFDREFEHRLEAMADQGRPLSVLMADIDSLKKI
                     NDAKGHLLGEYVVGEIGRIIGAMHEAEGRSATRFGGDEYQGVLPDLSKQEALRVAEEI
                     RRSVEAQTFEMDDVAATTTISIGVASYPEDGTTHQELTHAADAALYRAKRAGGNTVSN
                     "
     gene            1001..1588
                     /locus_tag="VFQ10_09785"
     CDS             1001..1588
                     /locus_tag="VFQ10_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HET9965674.1"
                     /translation="MTQPQAALPAEAGHWWALALRGAIAILFGLAALLRPDIALEALI
                     LLFGAYALVDGVFSIVGVFGGTRGGTPRWLLFIEGVAGILAGLIAFVLPGLTAILLLY
                     LIAAWAIVTGISEIVMAIRLRREIRGEWAMIVGGALSVLFGVILAVIGPVAGLFSLIW
                     LIGIYAVAFGILILITAFSVRGRDSGSADRPSRVV"
     gene            complement(1593..3038)
                     /gene="gatA"
                     /locus_tag="VFQ10_09790"
     CDS             complement(1593..3038)
                     /gene="gatA"
                     /locus_tag="VFQ10_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET9965675.1"
                     /translation="MPASLLDLTAYGLAKEIEGGEVSAREATDVANTRVEEVEDGVNA
                     FVTTTPELALERADAVDTRNGERALWEAVPLAVKDVLSTRGVRTTCGSRILENFEPLY
                     DASALLNFGEDLIMVGKANCDEFAMGSSTENSAWGPSRNPWDLGRVPGGSSGGSAAAV
                     AAGEGWWALGTDTGGSVRQPAALCGIVGLKPTYGRVSRYGLIAFASSLDVVGPMTRDV
                     RDAALLLQAIAGHDPRDSTSANVEVPDYLANLEDGMEGLRIGVVPELMGEGIEPGVRE
                     VVERVARELESAGAEVGEASLPHSGYGLPAYYIIAPAEVSSNLARFDGVRYGLRVPED
                     GVHEMYRKTREEGFGDEAKRRIMLGTYALSSGYYDAYYAQAQKVRTRIIEDFRAGFAR
                     FDVLLSPTAPSVAFEIGEKVDDPLAMYANDICAVPANLAGIPAISVPAGLFEGLPVGV
                     QLMGDHFTEPMLLRAARATEQVADFPFQMKP"
     gene            complement(3040..3321)
                     /gene="gatC"
                     /locus_tag="VFQ10_09795"
     CDS             complement(3040..3321)
                     /gene="gatC"
                     /locus_tag="VFQ10_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET9965676.1"
                     /translation="MISEEQVRHVANLARLGLTDDEIEKIGGQLDAILDSIEKIQELD
                     LAEVPPTANPLDLSNVLRPDEPRPELSQEQALSPAPERVDDLFAVPRID"
     gene            3594..>4513
                     /locus_tag="VFQ10_09800"
     CDS             3594..>4513
                     /locus_tag="VFQ10_09800"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HET9965677.1"
                     /translation="MTIHEAASESDRTGSGDAPGYQPIENYGIIGDLHTTALVGMDGS
                     IDWLCLPHHDSPSVFAAILDSVKGGRFKVSPEGGEVTTKQLYWPDTNVLVTRFFTPDG
                     VGEITDYMPIGAQESGHKRHQIVRRVEVVRGTMTFRMECSPAFDYARKEHETRIVPGG
                     ATFHSPDLSLGLASFFPLEQRDNGTTAQFTLQEGQSTVFVLREIEAGEDCGVCLSRMD
                     EEEIFMQTVQYWRRWLSKCTYTGRWREMVRRSALTLKLLTFEPTGAIVAAPTMGLPEA
                     IGGERNWDYRYTWLRDAAFTLYGLLRIGFT"
BASE COUNT          850 a         1452 c         1418 g          793 t
ORIGIN      
        1 cggccgcacc tcgtggtctt gcaaggaccc agtctcggcg acttcttcga gataaccgac
       61 gacgagctcg ttcttggcag cgacccgttc tcggctgaca tcgtggtgcg ggacgtggag
      121 gtcgaaccgc ggcacgccga actctacaag agacccggcg gctatgctct gcggggcgta
      181 gaagcaggga agactatcgc gctcaacggt gaggtaatgg aggagccagg gtgcggccaa
      241 ctcctctctg acggggaccg catacacgtg ggtgactccg tactcgagtt ctcgcaccag
      301 gatgagatca aagcctcctt tttcgaacag ttgcaccacc tcgtgaaccg tgaccacctc
      361 acgggcctcc tatccaaggg acgcttcgac cgcgagttcg agcaccgcct ggaggcgatg
      421 gccgaccagg gaagaccctt gagcgtgctc atggctgata tagacagcct caagaagata
      481 aacgacgcca aaggacacct gctcggcgag tacgtcgttg gtgagatcgg acgcatcatc
      541 ggcgccatgc acgaagcaga ggggcgtagc gccacccgct tcggcggcga cgagtaccag
      601 ggcgttttgc ccgacctctc caaacaggag gccttgaggg tagccgaaga gatacgtcgc
      661 agcgtcgaag cgcagacctt cgaaatggac gacgtggcag ccacgacgac catctccata
      721 ggcgtcgcca gctaccccga ggacggaacc acccaccagg aactgaccca cgccgccgac
      781 gccgccctct acagggccaa acgagccggc ggcaacacgg tgagcaacta agatcaacgc
      841 caggacatct ctcctgcggc gctcccctct ctaaggaccg caggcatacg gtggctgtcg
      901 gcagtgtcac ctttcccgag tccgtgcata taatgtgtct gacgaagggg catctttgca
      961 ccgttatgcc cgggtggcgg ccacgaaaga ggaggaaggt atgacccaac cacaggcggc
     1021 gttaccggcg gaggccggac actggtgggc tctggcgctg cgtggcgcca tcgccatact
     1081 cttcggcctg gccgcgctcc tgcggccgga catagccctg gaggcattga tcctgctatt
     1141 cggcgcctac gctctggtcg acggcgtgtt ctcgatagtg ggcgtgttcg ggggcacccg
     1201 cggggggacg ccccgctggc tgttgttcat cgaaggagtg gccggtatcc tcgcaggcct
     1261 gatcgccttc gtcctgccag ggctgaccgc gatcctcctt ctctacctca tcgccgcctg
     1321 ggccatcgtc acgggcatct ccgagatagt aatggccatt agactgcgca gggagataag
     1381 gggcgaatgg gccatgatcg tcggtggcgc attatccgtg ctcttcgggg tgatcctggc
     1441 cgtgatagga ccggtagccg ggttgttctc gctaatatgg ctgatcggga tctacgccgt
     1501 ggcgttcggg atcttgatac tgatcacggc cttcagtgta cgcggcaggg acagcggcag
     1561 cgccgacagg ccgagcaggg tcgtctgaag catcaaggtt tcatctggaa ggggaagtca
     1621 gctacctgtt cggtggccct ggccgcccgt agtagcatag gctcggtgaa gtggtcgccc
     1681 atgagctgga cgcctacagg gagcccctca aagaggcctg ccgggacgct tatggcaggg
     1741 atgccggcca ggttggcggg gacggcgcag atgtcgttgg cgtacatggc gagggggtcg
     1801 tccaccttct ccccgatctc gaacgccacc gagggcgctg tcggcgagag cagtacgtcg
     1861 aagcgggcga agcccgcccg gaagtcctcg atgatcctgg tcctgacctt ctgggcctgg
     1921 gcgtagtagg cgtcatagta gcccgaggac agggcgtagg tgccgagcat gatcctccgc
     1981 ttcgcctcgt ccccgaagcc ctcctcgcgc gtcttgcggt acatctcgtg cacgccgtcc
     2041 tcagggaccc gcaggccgta gcgcacgccg tcgaagcggg caaggttgga tgagacctcg
     2101 gccggggcga tgatgtagta ggctggaagc ccgtacccgg aatgtggcag gctcgcctcc
     2161 cccacctcgg cgccagcgct ttcgagttct cgggccaccc tctccacgac ctcgcgcacg
     2221 ccaggctcta tcccctcacc catcaactcg ggtaccaccc cgatccgcaa accctccatg
     2281 ccgtcctcga ggttcgccag atagtctggg acctccacgt tggcgctcgt cgagtcgcga
     2341 ggatcgtggc ccgcgatggc ctgcaacaga agcgccgcgt cccgcacgtc gcgggtcatc
     2401 ggcccgacca cgtcgagcga ggaggcgaag gcgatcaggc catagcgcga gaccctcccg
     2461 taagtgggct tgagcccaac gatgccacag agcgccgcag gctggcgcac ggagccgccc
     2521 gtgtccgtcc ccagcgccca ccagccctcg cccgccgcga ccgcagcagc agatccgccc
     2581 gaggacccgc caggaacacg gcccaggtcc caggggttgc ggctaggacc ccaggccgag
     2641 ttctccgtcg atgagcccat ggcgaactcg tcgcagttgg ccttgcccac catgatgagg
     2701 tcctcaccga agttcagaag cgccgaagcg tcgtagagtg gctcgaagtt ctcgagaatc
     2761 ctcgacccgc acgtcgtcct cacgccacgc gtagagagca cgtccttgac ggcgagcgga
     2821 accgcctccc agagcgcgcg ctcgccgttc ctggtatcca ccgcgtcggc gcgttcgagc
     2881 gccagctctg gggttgtcgt cacaaaggcg ttgaccccat cctctacctc ttcgacgcgc
     2941 gtgttggcga catctgtggc ctcgcgggcc gagacctcgc caccttctat ctcctttgcg
     3001 agcccgtagg cagtgagatc cagcaggcta gcaggcatct cagtcgatcc tggggacagc
     3061 gaagagatcg tcgacacgct cgggggccgg ggagagtgcc tgttcctgtg agagctcggg
     3121 gcgcggctcg tcggggcgta ggacgttcga gaggtcgagc gggttcgccg taggcggcac
     3181 ctcggcgagg tccagctcct gtatcttctc tatgctgtcc aggatcgcgt cgagctgccc
     3241 gcctatcttc tctatctcat cgtctgtgag tcccaaccgc gccagattcg caacgtggcg
     3301 cacctgctct tcggatatca tgccaggcct cccggtcttt agctcgtaga accagaacat
     3361 atcttagcgc ctgcgagcgc cacatccttc atctgaagcc accgctgttg cacgcgcggg
     3421 ccaacggggt tctaattgta caggcgccca cagatatctt cccgatggaa gattgactca
     3481 cccaggagcc gaggatatgg cccatcggag actctgtacg aggaaaaggg ctcatgagat
     3541 ggccggatga cggaagacga cagcgagacc ccctgcggag gaggagcaag gagatgacga
     3601 tccacgaggc agcaagcgaa tccgaccgca ccggttcggg ggacgcaccg ggctaccagc
     3661 ccatagagaa ctatgggatc atcggcgatc tgcatacgac ggccctggtg gggatggatg
     3721 gttccataga ctggctgtgc ctgccgcacc acgactcccc aagcgtcttc gcggccatcc
     3781 tcgacagcgt gaaaggtggg cggttcaagg tctcacctga aggaggcgaa gtcaccacca
     3841 aacaactcta ctggcctgat accaacgtcc tggtcacgcg gttcttcacg cctgacgggg
     3901 ttggagagat cacggactac atgccgatag gcgctcagga gagtggccac aagcgccacc
     3961 agatcgtccg tcgcgtcgag gtggtgcgcg gcaccatgac cttccggatg gagtgttccc
     4021 ctgccttcga ttacgcccgc aaagagcacg agaccaggat cgtccccggc ggggcgacct
     4081 tccactcccc tgatctcagc ctgggcctgg catcattctt ccccctggag caaagggaca
     4141 acgggacaac cgcccagttc acgctgcaag agggacagtc cacggttttc gtgctcaggg
     4201 agatagaagc tggagaagac tgcggggtct gtctctctag gatggacgaa gaagagatct
     4261 tcatgcagac cgtccagtac tggcgccgct ggctttccaa gtgcacctac accggtcgct
     4321 ggcgcgagat ggtccgccgc tcggccctta ccctgaagtt gctcaccttc gagccgacag
     4381 gcgcgatagt agccgcccct accatgggat tgcccgaggc catcggcgga gagcgcaact
     4441 gggactaccg ctacacctgg ctcagggacg cggcatttac cctctacggg ctcctgcgca
     4501 tcgggttcac aga
//
LOCUS       DASTKM010000239         7836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1486926, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000239 DASTKM010000000
VERSION     DASTKM010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486926"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="VFQ10_09805"
     CDS             <1..1807
                     /locus_tag="VFQ10_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET9965678.1"
                     /translation="LSIQEGGSTLTQQLIKNTYIAQQERQVPSFERKFVEGSLAWQYE
                     KEHSKGEILEQYLNTVYFGANAYGAEAAASTYFNKEASDLTLSESALLAGIINLPGIY
                     DPFVDPESAKERRNVVLDRMLEYGHISKKEHDRAVASKLDVSRGRVQHQNDNEYFLNA
                     VRKEIAEEYGDDMVYEGGLKIYTTLDPDLQAMANDAVDSIVNPEAGDPSASLVSIEPG
                     TGAVRAMVGGSDFDQVKFNLATQAHRQAGSSFKPFVYSEAVHEGISPETMYISKHLTI
                     DMGQNARPYEVDNYDFIHRGPITLEKALADSDNTVFVQLALDLGLQKVVEMANELGID
                     TEIDAYPSTAIGGLRVGVTPLEMASAYSTFANSGTHMEPYLVEKVMKDDKGKDTLLEE
                     HRPFGEEVLSNDEAAVVTEALRGVVERGTASYYHDLEAEIGRPSAGKTGTSNEFVDAW
                     FIGFIPQLSTSVWVGYPNERRSMTNINGLPEVNGENYPLDIWSLYMQSAVQKYPIQQF
                     DAPNPDLHLEVKTDGRTYVKPEPPPEPEKPKEENSGDTKGEDPGPENTGTRSEEPGSS
                     EEQPPSAPDSDQEPPSQQFPASASPASASPAPDGG"
     gene            1883..3067
                     /locus_tag="VFQ10_09810"
     CDS             1883..3067
                     /locus_tag="VFQ10_09810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9965679.1"
                     /translation="MKQTRGAQAAFARDSATWMTYGLVGFFAFMETVLGPIMPFLRRE
                     LDLGYAAASLHFSAFALGAVLLGFFGDRIVGRWGRRAALWGGAFGMAAGALLLILGQS
                     AWGTVPATFAMGICGALLIVTSQAVLSDRHGEYSAVALTESNVTASACAIIAPLLVGA
                     SAASGLGWRAALVVPAVALVLLAARYFSQPLDLRTNAAENDAPADAPTLPPLYWAFWV
                     LVALGVASEWCVGYWGADFLADGTGLTRPAAATSLTAYFAAMLAGRVVSSRLARTMPP
                     AILLAATLFLALFGFPLFWLSPGTLLALAGLFVTGLGIGGVYPLGVSAAIASAPGNTD
                     AAAARLSVGGGGAILVAPFVLGALADRVGISTAFGIVVPMLLAALVLALIAGRRGSQA
                     SV"
     gene            complement(3102..3947)
                     /locus_tag="VFQ10_09815"
     CDS             complement(3102..3947)
                     /locus_tag="VFQ10_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HET9965680.1"
                     /translation="MCSASSAAPRTCASTSTRNTLDTQDELHGFVCDVARRAGELQIS
                     RYQNPGEVREKAPKDFVTEVDLLCEVLMVNAIGERYPDDAILSEEMGGSVSESGRTWL
                     LDPVDGTANYMKANPMFCACVSVMEGGTVTHAAVAAPRLGDLYHARSGGGAYRESGGR
                     ETPLHVSDTKSLEYAIAGADLSLHGGEPQEPGVERVLLGAWQLRALGSAGIRGAWLAA
                     GYLDVSVGTRNTVWDYAPTVLLVSEAGGRATDLSGEPWNLSSEGLIATNATLHDEVLQ
                     TLHSA"
     gene            complement(3895..4998)
                     /locus_tag="VFQ10_09820"
     CDS             complement(3895..4998)
                     /locus_tag="VFQ10_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9965681.1"
                     /translation="MATQNSMMLAERTDELLNRGRSQGFLSTEEVANLLQEGDLSAAE
                     VEEFYTTLEEEEITIVDGEASENGGSRTTVAAAVHVTEAPAVQIAHAVATGDSIRMYL
                     AEIGRVPLLTHADEIMLAKGIARGCKRSKDRLVEANLRLVVSIAKKYRNRGVSFLDLI
                     QEGNLGLIRAAEKFDHSKGYKFSTYATWWIRQAITRAIADKGRTIRIPVHMVEKVNKY
                     HRTHRRMIQTLGREPSEEEIARELGVFVTEIQRLQEISQRSISLATPVGDDDSSELGD
                     FLEDAGSVTPTDAVSESLLKAHLREALDELPERERQIIELRFGMKDDRPRTLEEVGRE
                     FDITRERVRQIQMKTLNLLREQRRTQNLREYLN"
     gene            5358..6755
                     /locus_tag="VFQ10_09825"
     CDS             5358..6755
                     /locus_tag="VFQ10_09825"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET9965682.1"
                     /translation="MRGVGGVTHDSRCVEPGFAFVAIPGFKRDGKEFAPEAIRGGAVL
                     VVAESDVPGVPTAVVADARGALAVLAREVNGDPSRSMEIYGVTGTNGKTTTCYVLHAI
                     LARAYGEGECGLMTTAATISGGEIRPAVRTTPEATEVQGTLASMLRSGVRRVVMEVSS
                     HGLSLKRVAGTRFAGAIFTNLTRDHLDLHGSMEAYYAAKRGLFYMTEGPRLANVEDAY
                     GRRLAGELENVSTFGSMAEADYRIAEVETTRSRTSFALHHSEGVLGLQTPLLGAYNVL
                     NVAGAASLALAVGTDETAVSHAVRGMGQVPGRFERIAAAGEYGFEVVVDYAHTDVGLD
                     AVLRLARGIAGSRRRVICVFGAAGDRDGAKRPKMGRIASLLADMSLITTDDAYSEDPE
                     KIAREVEAGSDLSRTSIELDRRKAIRRALEAARTGDIVVVAGKGHERVQHLPEGDIPF
                     HDATVVRELLAEIRR"
     gene            6774..7034
                     /locus_tag="VFQ10_09830"
     CDS             6774..7034
                     /locus_tag="VFQ10_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET9965683.1"
                     /translation="MQRQTRSEVIRRLKSVEGHVRGVEKMVEEGDVTYADIVQQTNAI
                     FSAIRRINTVLLKDFVEERAERDGASEEMINDLIKAIDRVTK"
     gene            7182..7481
                     /locus_tag="VFQ10_09835"
     CDS             7182..7481
                     /locus_tag="VFQ10_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965684.1"
                     /translation="MEDRGKQQDDLGGLEEKVDELEKLTDSFDDVPEEDLVGTLGEAV
                     ELLAEINARIEARLDTAGDESREIGVLLGQVDFGPFDEAMEDHEHKERTTGEPGA"
     gene            7465..>7836
                     /locus_tag="VFQ10_09840"
     CDS             7465..>7836
                     /locus_tag="VFQ10_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965685.1"
                     /translation="MNRVPDLAPRIRDAALLEGDFVLSSGERSSFYVDKYLFSTEPNL
                     LRDVADALATAIPGGVERLAGVELGAVPLVVATALATGLPYVIVRKSAKEHGSSAGRS
                     IEGNLEKGERVALVEDVVTTGT"
BASE COUNT         1471 a         2430 c         2491 g         1444 t
ORIGIN      
        1 cctctccatt caggagggtg gctccaccct gacccagcag ctcataaaga acacttacat
       61 agcccagcag gagcgccagg tccccagctt cgaacggaag ttcgtcgagg gctcgctcgc
      121 ctggcagtac gagaaagagc actccaaagg tgagatcctc gaacagtacc tcaataccgt
      181 gtacttcggg gctaacgcct acggcgctga ggccgcggcc agtacctact tcaacaaaga
      241 agcctcagac ctcacgctct cagagtcggc gcttctggcc ggaatcatca atctgcccgg
      301 catctacgat cctttcgtcg atcccgagag cgcgaaagag aggcgcaacg tcgtgctcga
      361 cagaatgctc gagtacggcc acatctccaa gaaagaacat gacagggccg tggcatcgaa
      421 gctcgatgtg agccgtggca gggtccagca ccagaacgac aatgagtact tcctcaacgc
      481 cgtccgaaag gagatagccg aggagtacgg tgacgacatg gtctacgagg gtgggctcaa
      541 gatctacacc accctggacc cggatctcca ggccatggcg aacgatgcgg tcgactccat
      601 cgtcaacccg gaagccggcg atccttctgc ctccctggtc tcgatcgaac ctggaaccgg
      661 cgctgtgagg gctatggtcg gcggctcgga cttcgaccag gtgaagttca acctcgcgac
      721 ccaggcgcac cggcaggcgg gaagctcgtt caagcccttt gtctactcgg aggccgtaca
      781 cgagggtatc tcacccgaga ccatgtatat ctccaagcac ctaaccatag acatgggcca
      841 gaacgccagg ccttacgagg tggacaacta cgacttcatc caccgcggtc cgatcacact
      901 cgagaaggcg ctcgccgatt cggacaacac tgtattcgtc cagttggccc tggatctcgg
      961 cctccagaag gtggtggaga tggctaacga gctgggcata gacaccgaga tcgacgccta
     1021 tccttcgacc gcgatcgggg gtctcagggt cggggttacg cccctagaga tggcctcggc
     1081 atactcgacc ttcgccaact cgggtacgca catggaacca tacctggtcg aaaaggttat
     1141 gaaggacgat aaagggaagg acacgcttct ggaggaacac cgccccttcg gcgaggaggt
     1201 cctaagcaac gacgaggcgg ccgtcgtcac ggaggcgctg cgcggtgtgg tcgaacgcgg
     1261 aacggccagc tactaccacg atctcgaagc cgagataggc cgcccctccg cgggcaagac
     1321 aggtacctcg aacgagttcg tagacgcctg gtttatcggc ttcataccgc aactctccac
     1381 gagcgtctgg gtaggctacc cgaacgagcg gcgctctatg acaaacataa acggcctccc
     1441 tgaggtcaac ggcgagaact accccctgga catctggtcc ctctacatgc agagcgccgt
     1501 ccagaagtac ccgatccaac agttcgacgc accgaacccc gatttgcacc tggaagtgaa
     1561 gaccgacgga cgcacctacg tcaaacccga gcccccgcca gaaccggaga agcccaaaga
     1621 agagaatagc ggcgacacga aaggggaaga cccaggtcca gagaatacag ggaccagatc
     1681 agaagagccg ggttcctcag aggagcagcc accgtccgcg ccggactccg atcaggagcc
     1741 gccatcgcaa cagtttcctg cgagtgcttc tccagcgtcg gcctcacccg caccggacgg
     1801 aggctagaac gctcacgggg acagactttg agtcttcgcc gggccgcaag agtggtgcac
     1861 gcgtgaagtt caggagcctc ccttgaagca gacccgcggt gcgcaggccg ctttcgcgag
     1921 ggactctgcc acctggatga cctatggact ggttggcttc ttcgccttta tggagacggt
     1981 tctggggccg atcatgccct tcctcaggag ggagctggac ctcggctacg ctgcggcgag
     2041 cctccacttc agcgccttcg ccctcggtgc cgtgttgctc ggcttcttcg gagacaggat
     2101 cgtgggccgc tgggggagac gcgcagccct ctggggcggg gcattcggca tggccgccgg
     2161 cgccctactc ctgatcttgg gtcagagtgc ctggggcacc gtccctgcga ccttcgccat
     2221 gggtatctgc ggcgccctct tgatcgtaac ctcccaggct gtgctctccg acaggcacgg
     2281 agagtacagc gccgtagcac ttaccgagtc gaacgtcaca gcgagcgctt gcgcgatcat
     2341 cgccccgctc ctggtcggcg cgtccgcggc gtcggggctc ggttggcgcg cggcgctggt
     2401 cgtgcccgcg gtggcactcg tgctgctcgc cgcgcgttac ttctcccagc ccctcgacct
     2461 tcggaccaac gcggcggaga atgatgcgcc ggccgatgcc ccgaccctgc ccccgctcta
     2521 ctgggcgttc tgggtgctcg tggcgcttgg tgtcgcctcg gagtggtgcg tgggatactg
     2581 gggggcggat ttcctcgcgg acggaaccgg cctgacccgc ccggccgcgg cgacttccct
     2641 cacggcctac ttcgccgcga tgctcgcagg aagggtcgtg agcagccggc tggcgcgtac
     2701 tatgcccccg gctatcctcc tcgccgcgac cctcttcttg gcactcttcg ggtttccttt
     2761 gttctggctc tctccgggaa ctctcctggc cctggccggc ctcttcgtaa cggggcttgg
     2821 catcggcggc gtctaccccc tcggcgtctc ggcggccatc gcgtcggcac caggcaacac
     2881 cgatgcagcc gcggcgaggc tctccgtcgg aggaggcggc gccattctcg tggctccctt
     2941 cgtcctggga gccctggccg acagggtcgg cataagcacc gccttcggca tcgtagtccc
     3001 tatgctcctc gcagccttgg tcttggcact catcgcggga cgaagaggct ctcaggcgtc
     3061 agtctaaccg gaggcccttc gcggaggaac ctgcggcccg ctcaggcgct gtgcagtgtc
     3121 tgcaggactt cgtcgtgcag ggtggcgttg gtggcgatga ggccttccga ggagaggttc
     3181 cacggctcgc ccgagaggtc tgttgcgcgt ccccctgctt cggagacgag gaggaccgtc
     3241 ggggcgtagt cccagaccgt gttacgggtg ccgacggaga cgtcgaggta cccggccgcg
     3301 agccaggcgc ccctgatgcc cgcgctgcca agagccctta gctgccaggc tccgagtagc
     3361 actctctcta ccccgggttc ttgaggctct ccgccgtgca gagagagatc ggcgccggcg
     3421 atggcgtact ccaggctctt cgtatcgctg acgtgcaggg gcgtctctct accaccactc
     3481 tccctgtagg cgccgccccc ggatctcgcg tggtacaggt ccccgaggcg gggcgcggcg
     3541 acggcggcgt gggtaacggt gccgccctcc atgacggaga cgcaggcgca gaacatgggg
     3601 ttcgccttca tgtagttcgc ggtgccgtca acggggtcga ggagccaggt gcgcccgctc
     3661 tccgagaccg acccgcccat ctcctcggag aggatggcgt cgtcggggta gcgctcccct
     3721 atggcgttca ccatcaacac ctcgcaaagg agatccacct cggttacgaa gtccttcggg
     3781 gccttctctc ttacttcgcc aggattctgg tagcgcgaaa tctgaagctc cccagccctg
     3841 cgggccacgt cgcagacgaa cccgtggagc tcatcctggg tatccagggt gttcctagtt
     3901 gaggtactcg cgcaggttct gggtgcggcg ctgctcgcgg agcaaattca gggtcttcat
     3961 ctggatctgg cggacccgct cgcgcgtgat atcgaactcg cggcccacct cttccagggt
     4021 gcgcggacgg tcgtccttca tcccaaaccg gagctcgatg atctgacgct ccctctcagg
     4081 cagctcgtct agcgcctccc tgagatgggc cttgagcagc gattccgaga cggcgtccgt
     4141 gggggtcacg gagccggcgt cctccaggaa gtcgccgagc tcggaggagt cgtcgtcccc
     4201 gacgggggtg gcgaggctta tggaacgctg gctgatctcc tgcagcctct gaatctcagt
     4261 gacgaaaacg cctagctcac gcgcgatctc ctcctctgaa ggctcgcgcc cgagggtctg
     4321 gatcatgcgc ctgtgggtcc tgtggtactt gttgaccttc tccaccatgt gaacgggaat
     4381 gcggatggtc cgccccttat cagcgatggc gcgggttatg gcctgcctta tccaccacgt
     4441 cgcatacgtg gagaacttgt agcctttgga atggtcgaat ttctcggccg cccttatgag
     4501 ccccaggttg ccttcctgga tgaggtcgag gaacgaaacg ccccggttgc ggtacttctt
     4561 ggcgatggag acgaccagcc tcaggttggc ctcgacgaga cggtccttgg acctcttaca
     4621 ccccctggca atacctttgg cgagcattat ctcgtcagcg tgggtaagaa ggggcactct
     4681 tccaatctcg gcgaggtaca tcctgatgga atcccccgtg gctacggcgt gggcgatctg
     4741 gaccgcagga gcctcagtga cgtggacagc ggcagcaacc gtagttcggc tccccccatt
     4801 ctcggacgcc tcgccatcca cgatcgttat ctcttcttcc tctagcgtgg tgtagaactc
     4861 ctctacctcc gccgccgaga gatccccttc ctgcaagaga ttggcgacct cctctgtgct
     4921 gagaaagccc tgacttcgcc cccgattcaa aagctcgtca gtccgctccg ccagcatcat
     4981 ggagttctgg gtcgccattt atctcctctc ctgcgcctct gtgcaccaac tgacggcagc
     5041 cggacacaca tagacacaat tgtgaaatcc ctgtaaaaaa tgacccgcgt agcgtaacac
     5101 accatatatg gtgagtcacc ggatttcgac cctatttcgt gtgtattctt ttaccacatt
     5161 tccgccttct gaaaaccaag gcgtgtttcg gcgccgtttc aacacagttt cagcggtatt
     5221 tcaggcatat ttcggcgtgt tgagttctct tgagaaaatc tacatgaggt tctcgttatg
     5281 gctcttgcta cactagggcc gtgatccttg accgggcaaa gcttactcgg gttctcggga
     5341 cgaagatccc ttccggtgtg cggggcgtgg ggggcgtgac gcacgactct cgttgcgtag
     5401 agccgggctt cgccttcgtc gcgatccctg gcttcaagcg tgacgggaag gagtttgcgc
     5461 ccgaggccat tcgtggcggg gccgtcctcg ttgtggctga gagcgacgtt cctggtgtcc
     5521 cgaccgcggt cgtggcagac gcacgaggtg ctctggcggt gctggccagg gaggtcaatg
     5581 gcgatccttc gcggagcatg gagatctacg gcgttacggg caccaacggc aagactacga
     5641 cctgctacgt tctgcatgcg atcctcgctc gcgcctacgg tgagggcgag tgcgggctca
     5701 tgaccaccgc cgcgactatc tccgggggcg agatccgccc cgcggtaagg accacccccg
     5761 aggccacaga ggtgcagggg acgctcgcct cgatgctgcg gtcgggcgtc cgcagggtcg
     5821 ttatggaagt ctcctcgcac gggctatccc tgaagcgtgt cgcagggacg cgcttcgcgg
     5881 gggcgatctt caccaacctg accagggacc acctggatct ccacggctcg atggaggcct
     5941 actacgcagc caagcgtggg ctcttctaca tgaccgaagg ccccaggctc gccaacgtcg
     6001 aggatgctta cgggaggcgg ctggccggag agctggaaaa cgtgagtaca ttcgggagca
     6061 tggctgaggc ggattacagg atcgccgagg tcgagacgac ccggagcagg acgagcttcg
     6121 cgctccacca ttccgaaggc gtcctcgggc tccagacgcc gctgctcgga gcttacaacg
     6181 tgctcaacgt cgccggagcc gcctccctcg cgctcgccgt tgggacggat gaaacggcgg
     6241 taagccatgc agtgcgaggg atgggacagg ttcctggacg cttcgagcgg atcgcggcgg
     6301 ccggggagta cgggttcgag gtagtcgtcg actacgccca caccgacgtt gggctggatg
     6361 ctgtccttcg gttggcaagg gggatagcag gtagcaggag gcgagttatc tgtgtattcg
     6421 gggcggccgg agacagggat ggggcgaagc ggcccaagat gggacggata gcctcgcttc
     6481 tcgccgacat gagcctcatt acgacggacg acgcctactc tgaggacccc gagaagatag
     6541 caagagaggt cgaggccggc tcggatctct cacggaccag catcgagctt gacaggcgga
     6601 aggcgatccg gcgcgcgctg gaggcggcga gaaccggtga catcgtagtg gtagccggca
     6661 agggacacga gagggtccag cacctccctg aaggggacat ccccttccac gacgccacgg
     6721 tggtcaggga gctactggcg gagatccgac gatagaggga ggtagcgcaa gcgttgcaga
     6781 ggcagaccag gagcgaggtg atacgccggc tcaagagcgt ggaaggccat gtcaggggag
     6841 tcgagaagat ggtcgaggag ggcgacgtaa cctacgccga catcgtccag caaaccaacg
     6901 ctatcttctc ggccatcagg cgaataaaca cggtcctctt gaaggacttc gtcgaggagc
     6961 gggccgaacg ggatggcgcc tccgaagaga tgataaatga cctgataaag gccatagaca
     7021 gggtgacgaa gtgaaggtag gcaggtcggg ctttgatgtc attgatcgag tagtcctgcc
     7081 tttcgcggct tgctcgttga tggtcgcggc gctctcttgg gacgagcgcg ggtcttccgg
     7141 aggccgaaga cggggtcggc atggtgtata gtccggaacc aatggaagat cgaggaaagc
     7201 aacaggacga tctgggaggt ctggaggaga aggtcgacga gctcgagaag ctcaccgact
     7261 ctttcgacga cgtaccagaa gaagatctgg tcgggaccct gggcgaggct gtggaactat
     7321 tggccgagat caacgcacgt atagaagcca ggctcgacac cgcgggggac gagtcgcgcg
     7381 agatcggcgt gctactcgga caggtagact tcgggccctt cgacgaggcc atggaggatc
     7441 acgaacacaa ggagaggacc accggtgaac cgggtgcctg atctcgcgcc ccgcatcagg
     7501 gacgcggcgc tcctggaagg ggacttcgtg ctctccagcg gagagcgcag cagcttctat
     7561 gtggacaagt acctcttctc taccgaacct aacctcctgc gcgacgtggc cgacgcgctg
     7621 gctacagcta taccgggggg agtagagcgg ctcgccggtg tagagctcgg tgccgttccc
     7681 ctcgttgtgg ccacggccct cgccacaggg ttgccctacg tcatcgtccg caagtctgcc
     7741 aaagaacatg gcagctccgc cggacgcagt atcgaaggca acctggagaa gggcgagaga
     7801 gtagcccttg tggaggacgt cgtcaccacg ggtacg
//
LOCUS       DASTKM010000240         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1488079, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000240 DASTKM010000000
VERSION     DASTKM010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488079"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFQ10_09845"
     CDS             <1..621
                     /locus_tag="VFQ10_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9965686.1"
                     /translation="AFMLEKDENGEHHPGYSTELIKGKMGKRAVWQPDIHLEDVLVPD
                     ENRLEGANSFKDTNKVLTATRSGVAWEAIGHAIAAYEAALQYAQERVQFGKPIAGFQL
                     IQNKLSGMLAEITNMQLMCYRLAQLQEQGKMTGPMASLAKMNNARKAKQVCADARDIM
                     GGNGVLLEYHVARHLSDMEIVYTYEGTDTIQSLIVGRDVTGISAFV"
     gene            859..1875
                     /locus_tag="VFQ10_09850"
     CDS             859..1875
                     /locus_tag="VFQ10_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9965687.1"
                     /translation="MKAVRLHELEGPDELVYEEVPEPEPGPGETVVRLRAAALNRRDL
                     FATYGQYPGVTPDILPRIPGSDGSGEVVARGEGAEGPDEGTEVVINPALYWGDNPRMP
                     GKEYRILGIPDDGTFAQFIKVPADHVFPKPSHLSHEEAAALPLAALTAYRALVTRGGI
                     AEGDTVLVPGVGGGVATFLVQIASALGASVFVTSGSDEKIEDSKELGAEGGVNYNTED
                     WSRELKSMTGGVDLSADHVGGENFNALVSLAKPGGRIVVIGATAGPTSKAMTIRIALK
                     HLDVLGTAMGTAEEFGAMLDLYAEHGLRPKVNETFALEDTASALHLMEEGSGIGKIVL
                     DVPA"
     gene            1904..2329
                     /locus_tag="VFQ10_09855"
     CDS             1904..2329
                     /locus_tag="VFQ10_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9965688.1"
                     /translation="MTDPRIVSEPRGGAEDATFVRDGLALFNVAVTGDAYYSPLAIFL
                     KDERGAVLGGALGHVWGGWLDLDTLWVAEPFRGQGYGAKLLRAAEDEARMQGCRGVFL
                     TTFSFQAKPFYEKFGYEVVADIPDYPNGHSNHVLKKMLT"
     gene            complement(2645..2875)
                     /locus_tag="VFQ10_09860"
     CDS             complement(2645..2875)
                     /locus_tag="VFQ10_09860"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HET9965689.1"
                     /translation="MVSTNPEEAIVDFDPDDTAQYLEGVDYPASKEDLASAAEGNGAP
                     EELVERLRTLGRPTFSGPGEVVAELESSPTSG"
     gene            complement(2908..3138)
                     /locus_tag="VFQ10_09865"
     CDS             complement(2908..3138)
                     /locus_tag="VFQ10_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965690.1"
                     /translation="MTAERYTAVFTGLLSLKKPGEYLYVRMDDDPLRSGGYTVSRGRP
                     PYGELGREISFEDLPEGCKDLVLDMYHKMWNL"
     gene            3356..>3537
                     /locus_tag="VFQ10_09870"
     CDS             3356..>3537
                     /locus_tag="VFQ10_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965691.1"
                     /translation="MSGGFISRGFRGRRRRAEGDVRVPPGQYLVEGFPVLSAGPTPRT
                     PLEEWDFTIEGEVDST"
BASE COUNT          730 a         1057 c         1121 g          629 t
ORIGIN      
        1 gccttcatgc tcgagaagga cgagaacggc gagcaccatc caggctactc cacagagcta
       61 ataaagggca agatgggcaa gcgcgccgtc tggcaaccgg acatacatct agaagatgtc
      121 ctcgtccccg acgagaaccg cctcgaaggc gcaaactcat tcaaggacac caacaaggtt
      181 ttgactgcca cccgcagcgg cgtcgcctgg gaggccatag ggcatgccat cgcagcttat
      241 gaagcggcgc tgcagtatgc ccaggagcgg gtacagttcg gcaagccgat agccggcttc
      301 cagctcatcc agaacaagct ctcaggcatg ctcgccgaga tcaccaacat gcagctaatg
      361 tgctaccgcc ttgcccagct ccaggagcag gggaagatga ccggtccgat ggcctcccta
      421 gccaagatga acaacgcgcg caaggccaag caggtctgcg ccgacgccag ggacatcatg
      481 ggcggcaacg gagtcctgct ggagtaccac gtagcgcgcc acctctccga catggagatc
      541 gtctatacgt acgaaggcac agacacgatc cagtcgctga tagtgggtcg tgatgtgacg
      601 ggcataagcg ctttcgtata gaagttagtc agcacgctcc ggccttcggc ctccgcttgt
      661 cagcacggcg gcgccgcctt gtcagcactt cagcctgtca accaaaggct tctcgggtga
      721 tccgttaccc caacaaccga ccgggcataa tgagcgaatc gcctacgcga aagcatggga
      781 cgcgcattcg taataagctg actagctgac aagctgtgaa gcggcgtgct gacatgctga
      841 gcgactgaag ggagcgacat gaaagctgtt cgactacacg aactggaggg gcctgacgag
      901 ctcgtttatg aggaggtgcc tgagccagag ccggggccgg gcgagaccgt ggtccggctg
      961 cgggcggcgg cgctcaatcg ccgggatctg ttcgccacat atgggcagta ccccggcgtc
     1021 acgcctgaca tcctgcccag gattccgggc tccgatggtt caggtgaggt tgtagcccgg
     1081 ggggagggtg ccgagggacc ggacgagggg accgaggtag ttataaaccc ggcgttgtac
     1141 tggggagata acccgcggat gcctggcaag gagtaccgaa ttctcggtat tcctgacgac
     1201 ggtactttcg cccagttcat caaggtaccg gcggatcacg tcttccccaa gccctcgcat
     1261 ctttcgcacg aggaggccgc cgcgctgccc ctcgccgccc tgaccgccta cagggcgctt
     1321 gtaacgcgcg gcgggatcgc tgagggcgat accgtcctcg tgccaggcgt cggcggcggg
     1381 gtggcgacgt ttctggtaca gatcgccagc gccctcggtg ccagcgtctt cgtcacctcg
     1441 gggagcgacg agaagatcga ggattcaaag gagctcggtg ccgagggggg cgtaaactac
     1501 aacacggagg actggtccag ggagctcaag agcatgaccg ggggcgtgga tctctctgca
     1561 gaccacgtcg gcggcgagaa cttcaacgcg ctagtctcgc tcgccaagcc gggcgggcgg
     1621 atcgtcgtca tcggcgctac ggccgggcca acgtcgaagg ctatgaccat ccgcattgcg
     1681 ctaaaacatc tcgacgtgct ggggaccgcg atgggcaccg ccgaagagtt tggcgccatg
     1741 cttgacctct acgccgagca cggcctgcgt ccaaaggtaa acgagacctt cgcgctcgaa
     1801 gataccgcct cggccctcca cctaatggaa gaaggtagcg gaataggcaa gatagtcctg
     1861 gatgtacccg cctagtcctc gagagacgga cagggggtgg atcatgacag acccgaggat
     1921 cgtctcggaa cccaggggcg gcgcggagga cgccacattt gtgcgcgatg gattggcgct
     1981 cttcaacgtg gcggtcaccg gcgacgctta ctacagcccg ctcgccatct tcctcaagga
     2041 cgagcggggc gcggtgctcg gcggggccct tggtcacgtc tggggagggt ggctcgacct
     2101 cgacaccttg tgggtagcgg agccgtttcg cgggcagggg tacggagcga aactcctcag
     2161 ggctgccgaa gacgaagcca gaatgcaggg atgccgcgga gtatttctga ctacgttcag
     2221 cttccaggca aagccgttct acgaaaagtt cggatacgaa gtcgtcgctg acatcccaga
     2281 ttatcccaac ggccacagca atcacgttct gaaaaagatg ctgacttagt gggagtcaag
     2341 tcctgccgtg cgcagcggag acctcgaatt cgtaggtgtc gccgtgcagg ttcgagagca
     2401 agatgaaatc gagcggggga aggtcttcgt tctccacggc cggttgatcc ggcgcgtgga
     2461 ggtcaggccg tagcccaggt gtgcgtggtc gccggcgtgg aggaaattgg ggtcggtaag
     2521 gatagtaaac ccggcgtagc ggatcagaag cgtagcgttc ccgacaaaga agatggagcc
     2581 actcccgaac tccaggttct cgttcctcct gggaaggagt aaggagctcg cctctgtcac
     2641 aggctcatcc tgaggttggt gaagactcta gctctgccac aacctcaccc ggacccgaga
     2701 aggtgggacg gcccaaggta cgtagcctct ccacgagctc ctctggcgcc ccgttgccct
     2761 cagcggctga ggccaggtcc tccttactcg caggataatc gacgccttcc aagtactgcg
     2821 ccgtgtcgtc ggggtcgaag tccacgatag cctcctccgg gttcgttgac accaccctat
     2881 atgccccgga aaccgaggtt cgtaacgcta caggttccac atcttatggt acatgtccag
     2941 caccaggtcc ttgcaaccct ccggaagatc ctcgaacgag atctcgcgtc ccagttcccc
     3001 gtaaggcgga cggccacggc tcaccgtgta gccgccggaa cggagtggat catcgtccat
     3061 gcgcacgtac aggtactcgc ccggcttctt cagcgagaga agccccgtga acaccgccgt
     3121 gtagcgttcg gcggtcacgc cgccgtcgtc atagagtcag acgtagcgcg ggactccatc
     3181 aggcaagagc ttctctcgtc tattcccgaa ggccatccac ctattgtaat gccaacgaga
     3241 gtgaactctc gacaggctcg gggtttcctt tgacgaactc gcatttgacc ctgccttcgg
     3301 cggcgtgcaa gaatagatgt attcccggcg gagtacgacg gcaggaggta cattcgtgag
     3361 tggagggttc atttcccgcg gctttcgagg ccggcgcagg cgggctgagg gagacgtccg
     3421 agtcccgccg ggccagtacc ttgtggaggg cttccccgtc ctctctgcag gcccgacgcc
     3481 gcgcacacct ctggaggagt gggactttac gatcgagggc gaggtcgaca gtacgag
//
LOCUS       DASTKM010000241         6187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_784271, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000241 DASTKM010000000
VERSION     DASTKM010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784271"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..636)
                     /locus_tag="VFQ10_09875"
     CDS             complement(112..636)
                     /locus_tag="VFQ10_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9965692.1"
                     /translation="MRQTPDLSDVPIFEHLDEDERAEFEELSEPVRFEAGQRIFEEGA
                     PEERLYVITSGTVEVYKSVLPGRRQRLAIIEAPTVVGEMGLLTEPRAAASVEAMTMVE
                     AHGVGRDRLLEMLDADSPAACKLVYEIGRTLAERMAATNETVAEVISRLENRAGRSED
                     IDVFRDQLIRDWSF"
     gene            complement(649..1173)
                     /locus_tag="VFQ10_09880"
     CDS             complement(649..1173)
                     /locus_tag="VFQ10_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9965693.1"
                     /translation="MMEETLQGVRLFEFLTEEEREEFAEASEPVSFGPGENIIEEGAD
                     QESLFVLVSGTVEVRKGLSGGRSRLLAEIDASRGRTVLGERGLLGESGASATVMAVDE
                     VEAISIPRDTFRRMVAEGRPAAFKLSYRIARTLARRLTRLDEEVVEAIREIENRGETD
                     MEAFRDRLITDWSV"
     gene            complement(1390..1737)
                     /locus_tag="VFQ10_09885"
     CDS             complement(1390..1737)
                     /locus_tag="VFQ10_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET9965694.1"
                     /translation="MLLVAGAFAVSLAAFQDDALADTQLASWYGPGLEGNLTASGDVF
                     EPYTEYTAASPYLPFGTDLLVTYGGNSVVVEVTDRGPYVGGRDLDLSAVAAEEIGLTY
                     TGVDYVDVEVLNY"
     gene            complement(1958..2398)
                     /locus_tag="VFQ10_09890"
     CDS             complement(1958..2398)
                     /locus_tag="VFQ10_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965695.1"
                     /translation="MVLRRLRDIFGGRDRPTGEPSPLYGLEEEERARIGTEEDPLRNF
                     EAAMQRNEEAELAVEGGDTERAIKLYEISIREEFVGSHPYERLASLYESRHNLIEALR
                     VSEAFTKLAASGKMPRGAQRSADRKLPEFEARIQRYRRSLDEGR"
     gene            2517..4322
                     /locus_tag="VFQ10_09895"
     CDS             2517..4322
                     /locus_tag="VFQ10_09895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9965696.1"
                     /translation="MAYEFARYLKVRGAWGASWSPDGLKVTFLTEITGVPQVWEVASG
                     AQGPSWPEQLTFYEERISGALYSPAENRLLFVMDAGGNERAQIFLLEDGEVTNLTNAP
                     DAIHYPGDFSPDGRRIAYTATRRNGTDFDVYVQELPEGEPEMVWEVSGYHTVADWARD
                     GRALIVSRHHSNVNNDLYRLNLKTGEATLLTGHEGDARFSGANVTPDGESLYLATDRD
                     GEFLRLARLDLATLALEYLTPDDRDVEGVELSRDGRYLVASRNVDGYSDVLLFNGRGR
                     RMPDPRIPEGIVGSFEFSPDSRRLAFTLTAPERNPDIWVVDLPDGDARRLTRSSTAGI
                     PPRSFRKPRVVRYTSFDDHEIPGLFYEPETENAPVIVNVHGGPESQSRPAFAPVTQYL
                     LGRGYAVFFPNVRGSTGYGKAYTHLDDVGLRMDSVEDLSHAAYWLRERGHQRIAVMGG
                     SYGGFMVLAALTEYPDLWTAGVDIVGIANLVTFLENTGSYRRSLRECEYGSLEKDRAF
                     LESISPIHKAEDIKAPLMVIHGKNDPRVPVGEAEQIVDRVKANSGTVDYLLYEDEGHG
                     LAKLKNRLDAYPKIAAFLDRHLAAESESPKADLAR"
     gene            complement(4348..5967)
                     /locus_tag="VFQ10_09900"
     CDS             complement(4348..5967)
                     /locus_tag="VFQ10_09900"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HET9965697.1"
                     /translation="MQHLTGDQQEVEWQFDALDVRPVGRWLENGEGDNNPAIVAGETR
                     EISDTYLDTEDWRIYQAGYALRIRRVKGKNRAEATMKLLASEAEAPGLRSRREISEPL
                     DSAEPEAFDDDPGPVGKRIGTLVGPKKLRTLFEIHTRRNTYGLILEGSEVGEVALDET
                     TIPLENDAEPAQVRRVEIEVEPESLLRLEPFVERMRDACRLSPATASKYEAGLFARDL
                     APPGPPEFGPTGVDDSLTAGEVAFRILREQFAVFLTHEPGTRIGEDPEELHDMRVAAR
                     RMRAAMKIFEEPLPVRTQRFRNEFKWIAGALGEVRDLDVQLEQLDGWISSASSEDREP
                     LEALRAVFQERRKKARKSMLRRLNSRRYARLVESFGAFLERGPSRRAQASRRPILAAA
                     SDLVRKPYRKVRKLGDPLSEESSGEEYHELRKKGKRLRYALEFLSDIYGDPTKDLIKP
                     LKDLQDVLGDHQDAEVAVAHLRELAESKGRSPKLSPETIFVMGGIAHRYEVQARELRS
                     EFPGAYSKVRGKRWKKVKSAMEKARPKEETR"
BASE COUNT         1104 a         2032 c         1816 g         1235 t
ORIGIN      
        1 gagaagtgcc cggattgcgg cgccgacaag gagatgttcg acgaggtgcc ggtgccaggg
       61 tattagcacg ctacgccctt cgggcttcgc gtgctgaagt gctgaaatgc tctaaaagct
      121 ccagtcccgg atgagttggt cccggaagac gtctatatct tccgatctcc cggccctatt
      181 ctcgaggcgg gagattactt cggcgacggt ctcgttggtc gctgccatac gttcggcgag
      241 ggtacggccg atctcgtaga cgagcttgca ggctgccggg gagtcggcgt cgagcatctc
      301 gaggagccgg tccctaccaa cgccgtgcgc ctcgaccatg gtcatcgctt cgacgctcgc
      361 ggcggccctg ggttcggtga ggaggcccat ctcgccgacc acggtcggcg cctcgatgat
      421 cgccagtcgt tgccggcgtc ctggtaggac actcttgtag acctcgacgg tacctgaggt
      481 gatcacatac agccgctcct ccggggcgcc ttcctcgaag atcctctgcc cggcctcgaa
      541 cctcaccggc tctgaaagct cctcgaactc ggcccgctcg tcctcgtcca ggtgctcgaa
      601 gatcggcacg tcgctgaggt ctggcgtctg tcgcataaaa tgaaccccct atacggacca
      661 gtctgttatc agcctgtcgc ggaaggcctc catgtcggtc tcccccctgt tctcgatctc
      721 cctgatcgcc tcgaccacct cctcatccag acgcgtaagc ctcctcgcga gggtgcgggc
      781 gatgcggtaa gagagcttga aggcggcagg cctgccttct gcgaccatgc gcctgaaggt
      841 atcgcgtggg atacttatcg cctccacctc atcgacggcc atgaccgtag cggacgcccc
      901 gctctccccg agaagccccc tctcccccaa gacggtacgc ccccgtgagg cgtcgatctc
      961 cgccaggagc ctgctccttc ctcccgacaa gccctttctg acctcgaccg tgcctgagac
     1021 aagcacgaac aaactctcct gatctgcgcc ctcctcgatg atgttctcgc cggggccgaa
     1081 ggagactggc tccgatgcct cagcgaattc ctcccgctcc tcctcggtca ggaactcgaa
     1141 taacctgacc ccctgaagcg tctcctccat catcccatca ccctttcagc ctcgaagccc
     1201 ttggcgagat tatacgcacg ccttccgccc cggcgctccg tgccagaagc ccattgaaag
     1261 ttctcctgcg ttgcttggga tagcgaaggg tgtgaggatt aacccaaagg gaaacgggcg
     1321 cagcaaaaat gccgcgcccg ctctcgtgtc tcgccgttcg cctggcgaag acccttagtt
     1381 gaggacctct tagtagttga ggacctcgac gtcaacatag tcgacgccgg tgtaggtcag
     1441 cccgatctcc tcggcagcaa ccgcggagag gtccaggtcc ctgccaccaa cgtacggccc
     1501 acggtcagtg acctcgacga ccacggaatt gcctccgtag gtcaccagca agtccgtgcc
     1561 gaaaggcagg tacggtgagg ccgccgtgta ctcggtgtag ggctcgaaca cgtccccact
     1621 cgccgtcagg ttgccctcga gcccgggccc gtaccagctc gccagctggg tgtcggccag
     1681 ggcatcatcc tgaaaagcgg ccagcgatac ggcgaacgca ccagccacca acagcatctt
     1741 gagacaagac ttcaagagtc cccctctaga tcgcttgcac aacaagccca agaccttact
     1801 acgttacaat cgagttacaa cccgctacat atcgttttat gcgtctattt gtgaagcagt
     1861 tacatagacg gcggacctgc gctaagtggt atcagcgacg ctctttacct gcatatggtg
     1921 gtttttgctg ggtgaggcgt ggtgctggct tctgggtcta tcgcccttcg tcgagcgagc
     1981 gcctgtagcg ttggatgcgg gcctcgaact cggggagctt acggtcggcg gagcgttggg
     2041 ctccgcgggg catctttccg ctggcggcga gctttgtgaa ggcttcggat accctgagag
     2101 cttcgatgag gttgtgccgg ctttcgtaga gggaggcgag tcgctcgtaa gggtggctgc
     2161 cgacgaactc ctcgcggatg ctgatctcgt aaagcttgat ggcccgctca gtgtcgcctc
     2221 cctcgacggc cagctcggcc tcctcgttgc gttgcatcgc ggcttcgaag ttgcgaagcg
     2281 ggtcttcttc cgtcccgatc ctcgccctct cttcctcttc gagcccgtag aggggcgacg
     2341 gctcacccgt gggccgatcc cgcccgccaa agatgtcccg cagtcgtctg agaaccaagc
     2401 tcttccctcc cgataagctc gcctgagtat aaccaagggg tgagtctcgg tcgattcagg
     2461 ccttctctgt tgtgttttaa tctctgctcg ctggcaccga gtcaggaggg aacctgttgg
     2521 cctacgagtt cgcacgctat ctcaaagtaa ggggagcctg gggcgcaagc tggtcccctg
     2581 acggcctcaa ggtcactttc ctgacggaga tcaccggcgt gccccaggtg tgggaggtcg
     2641 ccagcggagc ccagggtccc tcctggcccg agcaactcac cttttacgag gagcgcatct
     2701 cgggcgccct atactcgccg gcggagaaca ggctcctctt cgtcatggac gccggcggca
     2761 acgaacgcgc gcagatcttc cttctcgaag acggcgaggt gacgaatctg accaatgctc
     2821 cggatgccat ccactaccct ggagacttct cgcccgacgg caggcgcatc gcgtatacgg
     2881 ctacccgccg caacgggacg gacttcgacg tctacgtcca ggaactgcca gaaggcgagc
     2941 ctgagatggt ctgggaggtc tccggctacc acacggtggc tgactgggct cgtgacggga
     3001 gggccctgat cgtctcccgc caccattcca acgtgaacaa cgacctgtac cgtttgaacc
     3061 tcaaaaccgg cgaggcgacg ctcctgacag ggcacgaggg ggacgccagg ttctccggcg
     3121 cgaacgtgac gcccgacggc gaaagcctct accttgcaac agaccgcgac ggggagttcc
     3181 tgcgcctggc ccgcctggac ctcgcgacgc tggcgctaga gtacctcacc ccggacgatc
     3241 gggacgtcga gggcgtcgaa ctttcgaggg acggccgcta cctggtcgcg agccgcaacg
     3301 tggatggcta ctccgatgtg ctgctcttca acggcagggg acgaaggatg cctgacccac
     3361 ggataccgga aggcatcgtg ggcagcttcg agttctcacc cgactcaaga cgactcgcct
     3421 tcaccctcac tgcccctgag cgcaaccctg atatctgggt cgtcgacctc cccgacggag
     3481 atgcgcggcg ccttacccgc tcctcgacgg caggcatccc gccccgcagc ttccgcaagc
     3541 cacgggtcgt gcgctatacc agcttcgacg accatgagat accaggcctc ttctacgagc
     3601 ccgagacgga aaacgccccc gtcatagtga acgtccacgg cggacctgag agccagtcac
     3661 gccccgcgtt cgctcctgtg acgcagtacc tccttggccg gggctacgcc gtgttcttcc
     3721 ccaacgtccg cggatcgacg ggatacggca aggcttacac gcacctcgac gacgtggggc
     3781 tgaggatgga ctccgtcgaa gacctttccc acgcggcgta ctggctgagg gagcgcggcc
     3841 accagcgtat cgcggtaatg ggaggttcct atggcgggtt catggtgctt gccgccctca
     3901 ccgaataccc ggatctgtgg acagcaggcg tcgacatcgt ggggatcgcc aacctcgtca
     3961 ccttcctgga gaacactggc agttaccgtc gctccttgcg cgagtgcgag tacggctcac
     4021 tggagaagga ccgcgcgttc ctcgagtcca taagcccgat ccacaaggcc gaggacatta
     4081 aggccccgct catggtcatc cacggcaaga acgacccccg cgtgcccgtc ggggaggccg
     4141 agcagatcgt cgatcgggtg aaagcaaaca gcggtacggt cgattacctc ctctacgagg
     4201 acgagggcca cggcctggcc aaactgaaga accgtctcga cgcctacccg aagatcgccg
     4261 ccttcctcga tcgccacctg gccgccgaaa gtgagagccc gaaagcagat ctcgctaggt
     4321 aagtcgaacg atctcccgac gggaaggtta tctggtctcc tctttcggac gagccttctc
     4381 catagcgctc ttcaccttct tccagcgctt acccctgacc ttgctgtaag cccccggaaa
     4441 ctcggagcgt aactccctgg cctgaacctc gtagcggtgc gcgattccgc ccatcacgaa
     4501 gatagtctct ggcgaaagct taggactgcg tcccttagat tccgccagtt ccctcagatg
     4561 ggcgaccgct acctctgcgt cttgatggtc cccgagcacg tcttgaagat ctttgagcgg
     4621 cttgatgagg tcttttgtgg gatctccgta gatatccgag aggaactcca gagcgtagcg
     4681 cagacgcttg cctttcttgc ggagctcgtg atactcctcg cctgaagact cttcactaag
     4741 agggtcgccg agctttctca ccttgcgata cggtttgcgg accagatccg aggcggcggc
     4801 cagtattggt cgtcgggagg cttgtgctcg acgggatggc cctcgttcca gaaacgcccc
     4861 gaacgactct acaagacggg cgtaacggcg ggaattgagc cttcggagca tggatttgcg
     4921 ggccttcttc cttcgctctt ggaacacggc gcgcagcgct tcgagcggct ccctatcctc
     4981 tgaggaggcg gaagagatcc agccatccag ctgttcgagc tgtacatcga ggtcgcgcac
     5041 ctcacccagg gctccggcga tccacttgaa ctcgtttcgg aacctctggg tccgaaccgg
     5101 gagcggctcc tcgaagatct tcatcgcggc ccgcatccgg cgggccgcaa cacgcatgtc
     5161 gtgcaactcc tccggatcct ccccgatccg cgtgcctggc tcgtgggtca gaaagaccgc
     5221 gaactgctct cgcagaatcc ggaaggccac ctctcccgcc gtgagcgagt catccacgcc
     5281 tgtaggccca aactcggggg gtccaggtgg tgcgaggtcc cgtgcgaaga gtcctgcttc
     5341 gtacttggag gcggtggccg gcgagagtct gcaagcgtcg cgcatacgtt cgacgaaagg
     5401 ctccagccgg aggagagatt ccggttccac ttcgatctcc acgcgccgaa cttgggccgg
     5461 ttccgcatca ttctctagcg gtatggttgt ctcgtcgagg gccacctccc ccacctccga
     5521 gccttcaagg atcaggccgt aggtgttccg gcgggtgtgt atctcgaaga gagtacgcag
     5581 ctttttcggt ccgacaaggg tgccgatcct cttcccgacc gggcctgggt catcgtcgaa
     5641 ggcttcaggc tccgcactat ctagtggctc ggagatctcg cgccggctac gaagccccgg
     5701 cgcttcggct tccgaagcca gcagcttcat cgtggcttcc gccctattct tgcctttcac
     5761 gcgccggatt cgcagggcgt agccggcttg gtagattcgc cagtcctcgg tgtcgaggta
     5821 ggtgtccgag atctcgcgcg tctcgccggc gactatggcc gggttgttgt ctccctctcc
     5881 gttttccagc caccgcccta cgggacgcac gtcgagggcg tcgaattgcc attcgacctc
     5941 ttgttgatct cctgtcaggt gttgcatggg tctggttatc gtctctcgtc tattaagctc
     6001 gtaaactagt catattagca tgcacagtcg tgtggtcatc cccctcccac tctacacggc
     6061 gctgtgcgga gcaatgctga agatcctatc cctcggcgag cgggaacgag gtgccagact
     6121 acacccgact aggtgagtac gggcatgacg ctgggccggc ggctcgtcac ccgcccctcg
     6181 gacgcca
//
LOCUS       DASTKM010000242        18732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_430575, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000242 DASTKM010000000
VERSION     DASTKM010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 18732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18732
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430575"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..599
                     /locus_tag="VFQ10_09905"
     CDS             393..599
                     /locus_tag="VFQ10_09905"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET9965698.1"
                     /translation="MPTRWGRAFCRGLRIFVATVVGMVADGMGEEEILKAYPDLERKD
                     VRETLQRTVEGVAPLDVSRHCDST"
     gene            complement(685..1752)
                     /locus_tag="VFQ10_09910"
     CDS             complement(685..1752)
                     /locus_tag="VFQ10_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HET9965699.1"
                     /translation="MQTYPIKLTAHVRSYYFGERLIPEMLGKKEVPEGVVAETWEISD
                     YREARATMTNGPYAGRTLHDLVVEFPQALVGEGWSGLHFPLLLKFLDASHMLPVHLHA
                     DDETARLKHGEPHGKTEAWHILWAAEGATILAGVKEGLGREELFEAFKAKDYDAVMPR
                     HPIRAGDTVYVPGGTIHTFGPDTLIFEVQQTSDLGQFVTPMSLYGEPLDEETWDANIN
                     AALDELKTDYLPRPNPGLILEDGPNRRVVCCAGPHFALERWTLREMRVEPSNPERCIT
                     LSNVGGPVRLEYPGGTETLVRAESCILPASMGEARVVPDGGEASLVLCYVPDLERDVV
                     ARLREAGYSDEDIRALGEVAV"
     gene            1859..2896
                     /locus_tag="VFQ10_09915"
     CDS             1859..2896
                     /locus_tag="VFQ10_09915"
                     /inference="COORDINATES: protein motif:HMM:NF025839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HET9965700.1"
                     /translation="MDLYGKALAPRELRRRVGRLGQVAGIEPFIFEEGPARGVRALRF
                     RTGGSLSFDVLCDRGMDLGAAEYRGAPLAWLSPTGVVAPHFRELWGEGWLRSFGGGLL
                     VTCGLQNVGEPSERDGEELGLHGRISNTPATLLSREARWDGEGCLLKARAEVRESQVF
                     GANLVLRRTISARVGEVCVQIEDTVRNEGHGTEPLMLLYHINLGWPLLDASARLVGPG
                     DPPEPRDAEARKGLENWDTFAAPMAGFRERVFYHRPRTDAEGWAEARLENPSLEGGIA
                     LSVRFRPEELPHFVQWTMTGEGTYVVGLEPATCRVGGYQKEEAEGRVIHLSPGESRRF
                     RLQIEVSQMRG"
     gene            complement(3406..3732)
                     /locus_tag="VFQ10_09920"
     CDS             complement(3406..3732)
                     /locus_tag="VFQ10_09920"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HET9965701.1"
                     /translation="MPFYLVQFSYTEQAVAHLVHHPQNRIEAVRPAFEEAGVTIRDSW
                     LAFGDYDVILVIEAPGNAQASAIAMAVTAGGAVRNYKTTPLMTWEEGIEAMRVAGGSE
                     YRPPGG"
     gene            complement(4403..5425)
                     /locus_tag="VFQ10_09925"
     CDS             complement(4403..5425)
                     /locus_tag="VFQ10_09925"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET9965702.1"
                     /translation="MERNGNDRSESGLLLLAEVAQLYYVRNLTQQHIAERIGVSRSKV
                     SRILREARARGIVEIRIHSPLTLAADLQDALKRCLGLRECLVLAAPQVDTDGSEGPDM
                     IGRLSALGARYLQENIADGSVVGVSWSSTVYHVVSARYLQEKSNVTVVQLMGSIGGSI
                     PELDGVSITARLADDWGVKAHYLHAPMLVADAVVRNGLLRDPHIKRTLEVARSADTMV
                     VSVGAIDRNSGQYRTGYLDDADLDYIRGRGAVGDICGSYFSYDGSPVGLRMNERTIAI
                     GFEDMKGIPNRIGVSGGMHKVRPNIGAARAGLLNVLITDQDTAREMLATLRSEDAVAS
                     SGDRRV"
     gene            5708..5848
                     /locus_tag="VFQ10_09930"
     CDS             5708..5848
                     /locus_tag="VFQ10_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965703.1"
                     /translation="MRSEWVSGTLAGLRLGGGEHAGLAAADDRIASRFEVPAAGAEVV
                     RA"
     gene            5845..6789
                     /locus_tag="VFQ10_09935"
     CDS             5845..6789
                     /locus_tag="VFQ10_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HET9965704.1"
                     /translation="MISTLTPNPSLDLTYELSGKLSRGAVQRAASVSVEAGGKGINVS
                     RNLVANGVPSRAVAPVGGPSGEQFLSLLEGVGVELVRVPVAGPVRTNTALAEKSGALT
                     KINASGPTLSEEEVERLLAETAAAARGAVWLAACGSLPPGAPDDLLARVVVAAHEAGC
                     KAAADSSGAPLAATLEAGPDLIKPNAEELAELVGKPLATFGDGLAAAKEACARGAKSV
                     LVSLGADGAILLNEDSAVHAQTPPFVPRSTVGAGDALLAGFLAADGEGESALIEAVAW
                     GAAAARLPGSRGPTPGDLDREAVQVQDTIDTKKRLKGE"
     gene            6795..7253
                     /locus_tag="VFQ10_09940"
     CDS             6795..7253
                     /locus_tag="VFQ10_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET9965705.1"
                     /translation="MSITIDDITSTNLVALDLEVAGQWAAIDALADLLDKDGRLSDRD
                     AYAEAVYAREKETGGTAMEMGIAIPHAKSAGVSQAGVAFGRTSTPLDYGEEKADLIFL
                     IAAPEGEHDLHVTVLQQLARRLVHESFRTSLREASTPEEVVELMREQIST"
     gene            7309..8766
                     /locus_tag="VFQ10_09945"
     CDS             7309..8766
                     /locus_tag="VFQ10_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HET9965706.1"
                     /translation="MKFVGVTACPTGIAHSAMAAEALETAAKEKGHDIGIEIHGASGA
                     EFVDRQVIREADAIIIAADATIAERNRFEGKPIVEVRLREAIDKPGEVIGRAEKAAEA
                     GESETSAGAVAQREGAEAEGGGGEEAVPTDMGGAGWGAEVRRWLMTGVSYMIPFVVAG
                     GILIALAFALGGAVEVTEIDVYENFTIPAMLYTIGSTAFGMLVPILAGFIAYAMADRP
                     GIAPGVVGGLIANEIGAGFLGGIAAGLLAGAVVMWLKKIKLPGGFGRIMPILIYPLIG
                     SLVVGALMIVVIGQPIASVTTGLENWLASLQGANAIVLGLILGSMMAFDMGGPVNKTA
                     YAFAIAGLEGGAFAPMAAVMAAGMTPPLALALATAIRGRLFTPAERRAGQANWVMGGS
                     FITEGAIPFAAADPLRVIPSLMVGSAITASLSLLFDCTLRAPHGGIFVIGLIGNWPLY
                     LLAIAIGTVVSAALVIGVKEFTGGADSEKATSAAA"
     gene            8816..9079
                     /locus_tag="VFQ10_09950"
     CDS             8816..9079
                     /locus_tag="VFQ10_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET9965707.1"
                     /translation="MVEKEAVVVPEAGLHARPATLFVKEAKSYGSDIVVIKDGVEANA
                     KSSLKLMTLGAKHGDKVVIRADGEDEEAAVDALVAILSEEEEE"
     gene            9084..10715
                     /gene="ptsP"
                     /locus_tag="VFQ10_09955"
     CDS             9084..10715
                     /gene="ptsP"
                     /locus_tag="VFQ10_09955"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET9965708.1"
                     /translation="MADYVKLDGVAASEGVAVGPAFVHVPHELKPERESISEDAVEDE
                     LKRFKNAVEAVVRKLSETAERLREGGSESEAGIFEAHAEMAEDPEFQDGVEERVRKLE
                     SPEAAVISVGEEFAGMFAAMEDEYLAARADDVRDVASQIATELMGGKKSGLETLAEPS
                     VILARNLAPSDTARIPKGIALGFVISEGSRTSHVSIMARSFGIPAVVGVGAALEEALG
                     AEIVAVEGTEGYAIADPDPDTISHFEQKQRDIAVEAALLEEYKHAEARTSDGRRIEVS
                     ANLGSAEEAEGALSWGAEGVGLFRTEFLFMKRTDLPSEEEQYEVYREVTEAFGEKPVI
                     IRTVDIGGDKDLPGVDQPEEENPFLGWRGIRMCLDVPELFKPQLRAILRAAPHGNLKI
                     MFPMVVDATELRAAKKVLEECREELKNEGAQFGDLDIGVMVETPAAAVRAKDIAPEVS
                     FFSIGTNDLVQYTLAADRGNERLTRLQSADHPAVLDLIGKTCEAAREAGIWVGVCGES
                     AGEPAMIPKLVELGVTELSMSAPSIPRAKKIVSEL"
     gene            10844..13531
                     /gene="ppdK"
                     /locus_tag="VFQ10_09960"
     CDS             10844..13531
                     /gene="ppdK"
                     /locus_tag="VFQ10_09960"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET9965709.1"
                     /translation="MKEQEAGRRTGGEAAEARAAGGFVYGFTEDLGSEDVLALCGGKG
                     SGLMRMRRLGLPVPEGFIITTEACVSYIESGELPEGLMAEVMDYLARLEEETGRGFGD
                     PENPLLVSVRSGAPISMPGMMDTVLNLGLGDATVRGLARSTGDERFAQDSHRRFIQVF
                     GEIVLKVPGHLFEEAIEELKRERGVEADTELTVQDLGELVGRFKSIVAREARVEFPED
                     PHKQLELAIGAVFDSWMGERAAAYRREYGIPESLGTAVTVQRMVFGNMGETSATGVAF
                     TRNPSTGEQGIFGEFLLNAQGEDVVAGIRTPLPLREMERVLPNAYHQFLETAERLERE
                     NQDMQDTEFTVERDTLYMLQTRSGKRTAEAALKIARDMAEEGLISREEAVMRVEPASL
                     DQLLHPRVDPEADLEVLAEGLPASPGAATGKIVLTAEEARERAEAGEDVLLVRRETHP
                     DDVEGMFAARGVLTALGGMTSHAAVVARGIGKPAVTGCGALKIDPTRGEISLDGTTLK
                     DGDVLTIDGADGRVIRGAVPLVQPQLGEDFETVLRWADEARGLGVRANADTPVDARRA
                     RELGAEGVGLCRTEHMFMEGERLRIMREMIVSESDEALGEALVQLEPMQREDFEGIFR
                     AMDGLPVTVRLLDPPLHEFLPESKDLAKEIALREARGESAEEQRRQLRVAESLEERNP
                     MLGLRGCRLGLLRPEIYLMQARAIAQAARAVREAGGEPVVEIMIPLVGFPSELRKMRD
                     LILEELAGESVPIGTMIELPRACAVAGEIARVADFFSFGTNDLTQMVCGISRDDAEEK
                     FLSDYLKSGILQENPFETLDQEGVGELVHMARERGRETNPHLKLGVCGEHGGDPKSVA
                     FFHEVGLDYVSCSPFRVPGARLAAAQAALKERSTTRQPV"
     gene            13611..14393
                     /locus_tag="VFQ10_09965"
     CDS             13611..14393
                     /locus_tag="VFQ10_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET9965710.1"
                     /translation="MEYGSGGPEMISAVLFDMDGTLLETEELKALSYARAAVELRPEH
                     VREADVLAAFGDLVGLSREEVASALMRRFGLEGAARERMHEFEADEPWQAFAGVRLRI
                     YEGLLGDPDLLLKQRYPHNIELLHELRRQGYPTALTTMSHRHHVRRVLSVLGLADAFD
                     VVVPRDDVRRGKPDPEIDLLAARKLGVPPEEFLVIEDSPAGVKAALAAGMVVIAVATT
                     LTRRGLRESGVLDPQRVVEDPRTLPAVVRRRIEAAGGKRVKE"
     gene            14442..15227
                     /locus_tag="VFQ10_09970"
     CDS             14442..15227
                     /locus_tag="VFQ10_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9965711.1"
                     /translation="MGFRGAIFDVDGVLVDSPHEQAWREGLHQLMENEWSDIQGQTSY
                     APERFTSEVYQRVMSGKPRYAGAQAALEHFGVPDAERRALEYGDRKQVMIVELIEAGE
                     FHAYPDALRFVLAVKDAGLHIAAASSSKNANAFLERIRLDEFVEEEGLSYGFVEPGYT
                     LLGILDANVSGRDFEQGKPHPEIFLTAAEELGFPAEECFVVEDAANGVQAAKAGNMAA
                     LGLARAEDEDLLKEANADLVVTTLDDVSLDALAEGRLEHVSVR"
     gene            15255..16955
                     /locus_tag="VFQ10_09975"
     CDS             15255..16955
                     /locus_tag="VFQ10_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965712.1"
                     /translation="MVARHTADFSEYQDAVEIALAGLKEERVVERIWAGDHTVWGLKP
                     EEIANRLGWLKSPQVMREELEGISALVEAVRNEGYTHALLLGMGGSSLAPEVYRNVFG
                     VADGHLDLAVLDSTDPDAVLEHADRLDLSRTLFVVSTKSGGTVETFSFFRYFYNRVSD
                     TLDEDRAGEHFVAVTDLGSGLQDIAEKYRFRATFLNDSNIGGRYSALSFYELVPAALI
                     GVDIRRLLDGAAEMARACGKSGVEENPGAWLGAAMGELANSVGRDKLTLVGGPTFAPL
                     GVWIEQLVAESTGKKGKGIVPVGSESPGAPEVYGDDRLFVYRGADGSDGEDSAGRLED
                     LAQAGNPVVRIEAREPYDLGGEMFRWMMATAVAGHILAINPFDQPNVESAKVLARKMV
                     AEYQEQGSLPGLQPTLQEHRITVYGSGEAKTAAEALEEFLSRARPGDYVALQAYVKPS
                     EETTSSLQGLRLRLRDRLRLATTVGYGPRFLHSTGQLHKGDAGNGLFVQFTADDERDA
                     PIPDEAGSAASSLTFGVLKEAQALGDRQALLDAGRRVIRFHLDEDVGGGLGRLTAWVS
                     "
     gene            17032..17838
                     /locus_tag="VFQ10_09980"
     CDS             17032..17838
                     /locus_tag="VFQ10_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9965713.1"
                     /translation="MRIVQLSDIHVGTPLFRPDFLKAAIEETNAYAPDFVAVAGDLTT
                     AGYRWEFEEAKSYLDQIECENVVVIMGNHDAKNVGYQHFEDLFGQRARSVTFSVPEGE
                     VKLVALDSSKPDLAEGEVGRENYGWIDAELRDWERGAKIVMVHHHLLAVPGTGRDRNN
                     LRDSGDMMAVLRDLKVDMVLSGHKHVPYVWSISGVRVIHSGTVSSMRVRGSIAPSYNV
                     IEFDSEKVLVNLRHPGEGEQPFARFSRQAVTTSEIFPDLEYFIRYDKASA"
     gene            17851..18663
                     /gene="otsB"
                     /locus_tag="VFQ10_09985"
     CDS             17851..18663
                     /gene="otsB"
                     /locus_tag="VFQ10_09985"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET9965714.1"
                     /translation="MTKNIRDLPDAVEHSDELAQRLAGRRPAVFLDYDGTLTPIVDRP
                     EDAVISESMRNAVRELAKRCPVCVVSGRDRRVVQELMGVDDLIVAGSHGFDIWSPAGG
                     EIQREEGEEFGGLLEGVEARLREELGGIEGALVEPKKSSVAAHYRLVSEDERPRVGRV
                     VEKILAEHPDGLKVTPGKMVYEVQPNIDWDKGKAVLYLLGALDLDRDDVVPIYLGDDI
                     TDEHAFEALATKGIGIFVGRADDPEVAGRTTAADYVLHDVPEVERFLDTLAR"
BASE COUNT         3409 a         5739 c         6439 g         3145 t
ORIGIN      
        1 ttacacgttg ctgcctagag gtggggggca acgtgaaggt tctggcgctg ctgtcgggga
       61 tactcggcag gactcgaacc tgcgaccttc tgattcgtag cgagccgttc cgtgcttctg
      121 tagactccca tcgggtcccg tttggcccct gtctacgagc ttttcgctcc gggacgacgt
      181 cgttagatcc ccccaaatcc tcgtggaccc gcgcccataa gtggtagcgt aagtggtaat
      241 aaatgccctg tctcgagccc gtataaccca cggcctcgcg gtgcacgaag ctcaaggggc
      301 gcccgtgcgg agcgtcgagt acaatgacaa ttgtaaggaa gtgcttccga aaggtggcgt
      361 cgacgtcgtg gccttcgatc gcataatggt ggatcccgac aagatggggg cgcgccttct
      421 gccggggctt gcgcatcttc gtggcgacgg tggtcgggat ggtagctgac ggcatgggcg
      481 aagaggagat cctcaaggcc taccccgacc tggagcgcaa ggacgttcgt gagacgctac
      541 agcgtacggt cgaaggtgtc gccccgctgg acgtgtcccg gcactgtgat tcgacctagg
      601 agggcgtctc ctcccggtag acatccgcca ccaccccggg cagggtcact atcttgccca
      661 cggcttcacc tccgtccgta cctgctaaac agcaacttct cccagtgccc gtatgtcctc
      721 gtcggaatac ccggcttccc gcaggcgtgc gaccacgtcc cgctccagat ccggcacgta
      781 gcagaggacg aggctcgcct ctcctccatc gggaaccaca cgcgcctcgc ccatagaggc
      841 cggcagtatg cagctctcgg cgcgcacgag cgtttccgtc ccgcccgggt actcgaggcg
      901 caccggcccc ccgacgttcg agagggtgat gcaacgctcc ggattggagg gctcgacgcg
      961 catctcccta agcgtccagc gctcgagggc gaagtgcggg ccggcgcagc acactacgcg
     1021 gcggttgggc ccgtcctcga ggatgagacc ggggttgggg cgcggcaggt aatccgtctt
     1081 caactcgtcg agggcggcgt ttatgttcgc gtcccaggtc tcctcgtcga gcggctcgcc
     1141 gtagaggctc atcggggtca cgaactggcc gaggtcggag gtctgctgga cctcgaagat
     1201 cagggtgtcc ggcccgaagg tgtggatggt cccgccggga acgtagacgg tgtcccccgc
     1261 tcgtatggga tggcggggca taacagcgtc gtaatctttg gccttgaagg cttcgaagag
     1321 ctcctcgcga ccgagcccct cctttacgcc ggccaggatc gtcgctccct cggcggccca
     1381 gaggatgtgc caggcctcgg tcttgccgtg aggctcgccg tgcttcaagc gggccgtctc
     1441 gtcgtcggcg tggaggtgga cggggagcat gtgcgaggca tcgaggaact tcaaaagcag
     1501 cgggaagtgc aggccgctcc agccctctcc gacgagcgcc tgcgggaact cgacgacgag
     1561 gtcgtggagc gtccttccgg cgtagggacc attggtcatg gtggcgcgcg cctcgcggta
     1621 gtcgcttatc tcccacgtct cggccacgac accctcgggg acttccttct tgcccagcat
     1681 ctcggggatc agacgctccc cgaaatagta ggagcgcacg tgggctgtca gctttatggg
     1741 ataagtctgc atcgcttctc cgtcctgcta tccgggatcg ccctttcccg atccacgttc
     1801 ccgtcccggt gagggtattc tactccgggg cgtcgttcga ggtagggagg tgggtagcat
     1861 ggatctctac ggcaaggccc tggctccccg ggagctgcgg cggcgggtag ggaggttggg
     1921 acaggtggcc ggcattgagc cgttcatctt cgaggagggc cccgcgcgcg gcgtgcgagc
     1981 cctccggttc aggacgggcg gcagcctctc cttcgacgtg ctctgcgacc gggggatgga
     2041 tctcggcgcg gccgagtacc ggggagcgcc gctagcctgg ctctcgccga ccggcgtggt
     2101 ggcgcctcac ttccgggagc tttgggggga gggctggctc cggtcgttcg gcggaggact
     2161 gctcgtcacc tgcgggctcc agaacgtcgg cgagccctcc gagagagacg gagaggagct
     2221 cgggttgcac ggtaggatct ccaacacccc cgccaccctc ctctcgcgcg aggcccggtg
     2281 ggacggggag ggctgtctcc tcaaggcgcg cgctgaggtg cgcgagagcc aggtgttcgg
     2341 cgccaacctg gttctgagac gcacgatctc tgcccgcgtc ggggaggtat gtgtgcagat
     2401 cgaggacacc gtgcgcaacg aaggccatgg cacggagccc ctgatgctcc tctatcacat
     2461 aaacctcggc tggcccctcc tggacgcgtc ggcccgcctc gtgggccccg gagacccacc
     2521 ggagccgcgc gacgccgagg cgcgcaaggg acttgaaaac tgggatacct tcgcggcccc
     2581 gatggcgggg tttcgtgagc gtgtcttcta ccaccgcccg cggacggacg cggaaggttg
     2641 ggccgaggcg cgcctggaaa atccgtcgct cgaagggggg atagccctct cggtgcgctt
     2701 ccgccccgag gagctgcccc acttcgtgca gtggaccatg accggggagg gtacctacgt
     2761 ggtcgggctg gagccggcga cctgccgggt cggcgggtac cagaaggagg aggcggaggg
     2821 acgcgtgatc catctctctc ccggcgagtc gcggcgcttc cggctgcaaa tcgaggtctc
     2881 ccagatgcgc gggtgagcgc ggtgcggttc ggcgtctacc tcctccaggg tgcggcccct
     2941 gttctggggg cgccgaacgt cggtcgatcc ggcgcattcc ccaaatgtat cgtcgtacgc
     3001 aacactccga ggtaaaaatc ctcatactca gcggcatcct caacttctcc accctggcgg
     3061 gcaacgcaag gcacggtttg tcgtcctgtc ctgcgaaagg tcggacggga cggagtggcg
     3121 ctgttctcct ggatcgacgc cgacggttca cccgggtggc tcgatcgcgg ctgctacctg
     3181 ttagcgcctg gatggaaggc cggacgatgg gcttcgagca agcggtagcg tgcgccttga
     3241 aggacggaag ggataccggc tgagtcttat cggtagtttg ttgaggcggg atatgggcgg
     3301 cttcttgaac caagagtcct ttgcgggtcc gagccaaaag tggccgtcct aaactaaaaa
     3361 gctggggatg ttgcgcctcc ggcccttttc cccggtcgtt ctcggctagc caccaggtgg
     3421 tcggtattcc gagccgcctg ccacccgcat cgcttcgatc ccctcttccc acgtcatcag
     3481 cggcgtggtc ttgtagttcc gtaccgcacc tcctgccgta acggccatgg cgatagccga
     3541 tgcttgcgcg ttgcctggcg cctcgatgac aaggatcacg tcgtaatccc cgaaagcaag
     3601 ccaagaatcg cggatcgtaa caccagcctc ttcgaacgct ggtctcactg cttcgatgcg
     3661 gttctgcgga tggtggacta aatgggccac cgcttgttcg gtatagctaa attggaccaa
     3721 atagaacggc acccttaccc ttctcctttt ctcgctctcc ccggtggctt gaacgccaca
     3781 ctgtaatctt gcctccatac gaagctaggc gcaaccgcac gtgcggctgc gctagcttca
     3841 gggagcggac tgccccaggg gtgtctcgat ggcgggttgt ttggcggtca gctgtgcacc
     3901 aatagctttc tcgacctcgc tgtagctcgt cgggttcctt gttgtccttc acggtttcaa
     3961 ggacgttcca caacagctcg accgacaccc cgacggtggt cagattcgtc tggtataaag
     4021 gcccgccacc tcgacgccgc gggccttggc caatttacgc agatcagcag catgccaaac
     4081 attaaggcta gccggaccga tatcattgcg acccgctata gcggtagttt gttgaggagg
     4141 gatatgcgca ggctgggagt gctcggaatg cgcttgctcg gtagagtcgt aaatacgctt
     4201 aaatcaaccg tagataggct cttttcctac ttcaacagac caccgattac gcgtcgtgcg
     4261 ccgaggtata gatcactacc gtgcgattcc cttggaatta tccggtaact ccgagcagtc
     4321 gctcgctccg atgacgccga gttgagctta ccccccttcg gaggacggga accaagcggc
     4381 ctggagtagt ccggaccgca gcctataccc tgcggtcgcc tgaggacgct acagcgtcct
     4441 cgctcctcaa ggtggccaac atttccctgg ctgtgtcctg gtccgtaatc aacacgttga
     4501 gcaatcccgc gcgcgccgcc ccaatgttcg gtcgaacctt gtgcattccc ccgctgacgc
     4561 cgatccggtt ggggatgcct ttcatgtcct caaaacctat ggctatcgta cgctcgttca
     4621 tccgaagccc aactggggag ccatcgtacg aaaaatagga gccacagata tcgccaaccg
     4681 ctccgcgccc gcggatatag tccagatcgg catcgtcaag ataaccggta cgatattgtc
     4741 ccgagtttcg atctatagca ccaaccgaca ccaccatggt atccgcgctt ctggctacct
     4801 ctagggtcct cttgatatgc gggtcacgca atagtccgtt gcgcacgacg gcatcggcca
     4861 cgagcatggg agcgtgcaga taatgcgctt ttacgcccca gtcatcagcg aggcgtgcgg
     4921 tgatggatac cccatctagc tcgggtatgg acccgcctat gctgcccatc aactggacca
     4981 ccgtcacgtt gctcttctcc tgcaagtacc tggcgctcac aacgtggtag acggtgctgc
     5041 tccagctcac ccccaccacg ctgccatcgg caatattctc ctgcaggtag cgtgcgccaa
     5101 gcgcactcaa cctgccgatc atgtctgggc cctcagaacc gtcggtatcc acctgcggag
     5161 cagccaagac aagacattcg cgcagaccga gacacctctt gagtgcatcc tgcaaatctg
     5221 ctgcaagcgt cagcggcgag tgaatccgta tctcgactat gcccctggct cgcgcctctc
     5281 tcaatatccg cgataccttg gaccgcgaga ccccgatgcg ctcggcaatg tgctgctggg
     5341 tcagattcct gacgtagtag agctgcgcta cctcggccag cagcagtaac ccgctctccg
     5401 accgatcgtt cccgttacgc tccaccaaat cgacatcctc tcgtgaaaaa aaccctcttg
     5461 attatgccac gggtcaacaa ttctagtact gcgctcgacc ttgtctaaac gccaagcccc
     5521 ggaacccccg cccgtttact tgcacaaatg atcagaacat tgcacacttg acgtgaacag
     5581 gcggtagcag tatgctgggg acataaggag ccgtaccggc actgggggta cggaagctgt
     5641 ggggcgaggg gctgtggata gccgacggca aatggaacga cgcggtttgc caggattgcg
     5701 tacttctgtg cgatccgagt gggtgagcgg tacgctcgct gggttgcggc tgggcggtgg
     5761 cgagcatgcc ggattggcag cggccgacga ccgtatcgcg agtcgcttcg aagtccccgc
     5821 cgccggagcg gaggtggtac gcgcgtgatc tcaacgctca cgccgaatcc tagcctggat
     5881 ctgacctacg aactatccgg aaagctatcg cgcggcgccg tacagcgggc cgcctccgtc
     5941 tctgttgagg ccggcggcaa gggcatcaac gtctcgcgca acctcgtagc caacggcgtc
     6001 ccctcgcggg ccgtcgcgcc cgtcggagga ccgagcgggg aacagttcct atcgctgctc
     6061 gaaggcgtgg gggtggagct ggtgcgcgtg cctgtcgccg ggccggtcag gaccaacacg
     6121 gccctagcgg agaagagtgg ggcgctcacc aaaatcaacg ctagcggtcc cacgctctcc
     6181 gaagaggagg tcgagcggct gctcgcagag acggccgccg ccgcgcgtgg cgcggtttgg
     6241 ctcgcggctt gtgggagcct gccgcccggc gcgccggacg atctgctcgc gcgcgtggtg
     6301 gttgccgccc acgaggctgg ctgcaaggcc gccgcggaca gcagcggcgc accgctcgcc
     6361 gccaccctcg aagcgggacc agaccttatc aagcccaacg cggaggagct cgccgagctc
     6421 gtgggcaaac cgctcgcaac cttcggcgac gggctcgccg ccgccaagga ggcgtgcgcc
     6481 cgcggcgcga agagcgtcct cgtctcgctg ggggccgacg gcgcgatcct cctaaacgaa
     6541 gacagcgcgg tgcacgccca gaccccaccg ttcgtcccgc gcagcacggt aggcgcgggc
     6601 gacgccctgc tcgccgggtt cctcgctgcg gacggcgagg gggagtccgc cctgatcgag
     6661 gcggtcgcgt ggggcgcggc ggccgcgcga ttacccggct cgcgaggacc cacgccgggg
     6721 gaccttgatc gggaggctgt tcaagttcaa gataccattg atacaaaaaa gcgattgaaa
     6781 ggagagtaaa tcggatgagc atcaccattg acgacatcac cagcaccaac ctggtcgcac
     6841 tggacctgga agtcgctggc cagtgggcgg caatcgacgc gctcgccgac ctgctggata
     6901 aagacggacg gctctccgac cgcgacgctt acgccgaagc ggtatatgcg cgggagaagg
     6961 agaccggcgg cacggctatg gagatgggga tcgccatccc tcacgcgaaa tctgccgggg
     7021 tgtctcaggc gggcgtcgca ttcggacgta ccagcacgcc gctggactac ggtgaggaga
     7081 aggcggattt gatcttcttg atcgccgcgc ccgagggcga gcacgacctg cacgttacgg
     7141 tgctccagca gctcgcccgc cgcctcgtgc acgagagctt ccgtacttcg ctgcgggagg
     7201 cgtcaactcc agaagaggtt gtagagctca tgcgcgagca gatctccacc tagagtaaga
     7261 ggagcaaccc actccgcggg ataagactaa agaaggagtt gtagcggcat gaagttcgta
     7321 ggagttaccg cctgcccgac aggcatagcc catagcgcga tggccgccga ggcacttgag
     7381 accgcggcca aggaaaaggg ccacgacatc gggatcgaga tccacggcgc ctccggcgcg
     7441 gagttcgttg accggcaggt gatccgggag gccgacgcga taatcatcgc cgccgacgcc
     7501 accatcgccg aacgcaaccg tttcgagggg aaaccgatcg ttgaggtccg gctcagggag
     7561 gccatagaca agcccggcga ggtcatcggg cgcgcggaga aggccgccga ggcaggcgaa
     7621 agcgagacga gcgcgggggc agtggcgcaa agagaaggcg ccgaagcaga aggaggcggc
     7681 ggagaggagg cggtgccgac ggacatgggc ggcgccggat ggggcgcgga ggtccgccgc
     7741 tggctcatga ccggtgtcag ttacatgatc ccgttcgtcg tggcgggcgg aatccttatt
     7801 gcactggcct tcgccctggg cggcgcggtc gaggtgaccg agatcgacgt ctacgagaac
     7861 ttcaccatac cggcgatgct ctacactata ggatctactg ccttcgggat gctcgtgccg
     7921 atcctggccg gattcatcgc ctacgcgatg gccgaccgtc ccggcatagc ccccggcgtg
     7981 gtgggcggcc tgatcgccaa cgagatcgga gccggctttc tgggcggcat cgccgcaggc
     8041 ctcctggccg gagccgtggt catgtggctc aagaagatca aactgcccgg cgggttcggc
     8101 cggatcatgc ccatcctgat ctaccctctc atagggagtc tggtggtcgg ggcgcttatg
     8161 atcgtggtta taggccaacc tatcgcctcg gtgaccaccg ggcttgagaa ctggctggca
     8221 agcctccaag gggccaacgc gatcgtgctg ggcctcatcc tcggttcgat gatggccttc
     8281 gacatgggcg gcccggtgaa caagaccgcg tacgccttcg ccatagccgg gcttgaggga
     8341 ggcgccttcg cgccgatggc ggcggtgatg gccgccggca tgaccccacc gctggctctg
     8401 gcgcttgcta ctgccatccg gggaaggctc ttcacccccg cggagcggcg ggccggacag
     8461 gcgaactggg tgatgggggg ctcgtttatc accgagggcg cgattccgtt cgccgcggcg
     8521 gacccgctgc gcgtcatccc gtcgctgatg gtcggctcgg cgatcacggc ctcgctgtcg
     8581 ctcctgttcg actgtaccct gcgggcgccg cacggcggaa tcttcgtgat cgggctcatc
     8641 ggcaactggc cgctctacct cctcgctatc gccatcggta cggtcgttag cgcggcgctc
     8701 gtgatcggag tcaaggagtt cacgggtggg gcggactcgg agaaggcgac atccgccgcc
     8761 gcctagacgg cgagcacgct ttagcgcggc aaggagcgaa aggaaggaac gaacgatggt
     8821 ggaaaaagag gcggtcgtcg tccccgaagc gggacttcac gcccggccag caaccctgtt
     8881 cgtgaaggaa gccaagagct acggctcaga tattgtggtc atcaaggacg gtgtagaggc
     8941 gaacgccaag agctccctga agctgatgac gctcggggcc aagcacgggg acaaggtcgt
     9001 gatccgggcc gatggcgaag atgaggaggc agccgtggat gccctggtcg ccatcctctc
     9061 cgaagaagag gaggagtagc ctcgtggcgg attacgtgaa gctcgacggc gtcgccgctt
     9121 ctgaaggggt cgccgtcggc ccagcctttg tgcacgtccc ccacgagctg aaacccgagc
     9181 gcgagagtat ctctgaggac gcggtcgagg atgagctgaa acgtttcaag aacgccgtcg
     9241 aggccgtggt tcggaagctc tcggagacgg cggagcggtt gcgggagggc ggcagcgaga
     9301 gcgaggccgg gatcttcgag gcgcacgccg agatggccga agaccccgag ttccaggacg
     9361 gcgtcgagga gcgggtgaga aaactggaga gcccagaggc cgccgtgatc tccgtgggcg
     9421 aggagttcgc gggtatgttc gccgcgatgg aggacgagta cctggcggcc cgggccgacg
     9481 acgtgcgcga cgtagcgtcc cagatcgcca ccgagctcat gggcggcaag aagtccggcc
     9541 tcgaaaccct ggcggaaccc tccgtgatcc tggcccgcaa cctcgcccct tcggacacgg
     9601 cccgcatccc gaaggggata gccctgggct tcgtcatctc cgagggatcg cggacctcgc
     9661 acgtctccat catggcccgc tccttcggca tcccggcggt ggtcggggtc ggagcggcgt
     9721 tggaagaggc actgggggct gagatcgtcg ccgtggaggg cacagagggc tacgccatcg
     9781 ccgacccgga cccggacacg atctcccact ttgaacagaa gcaacgcgac atcgccgtgg
     9841 aagccgccct gctcgaggag tataaacacg cagaagcacg caccagcgac ggtcgccgca
     9901 tagaggtctc cgctaacctg ggctcggccg aagaggcgga gggcgcgctt tcgtgggggg
     9961 cggagggcgt cgggctcttc cgcactgagt ttctgttcat gaagcggacg gacctgccct
    10021 ccgaggagga gcagtacgaa gtataccgcg aggtcacgga ggcgttcggc gaaaagcctg
    10081 tcatcatccg caccgtagat atcggcgggg ataaggacct gcccggagtg gaccaacccg
    10141 aagaggagaa cccgttcctt gggtggcggg ggatcaggat gtgcctggac gtaccagagc
    10201 tcttcaagcc ccagctgcgc gccatcctgc gcgccgcgcc ccacggcaac ctgaaaatca
    10261 tgttccccat ggtcgtggac gccaccgagc tgcgcgccgc aaagaaggtc ctcgaagagt
    10321 gccgcgaaga gctcaaaaac gaaggagcgc aatttggcga ccttgatatc ggggtgatgg
    10381 tcgagacacc ggcggcggcg gtcagggcca aggacatcgc ccccgaggtc tccttcttct
    10441 ccatagggac caacgacctg gtgcagtaca ccctggccgc ggacaggggt aacgagcgcc
    10501 tgacgcgcct tcagagcgcc gaccatcctg cggtgctgga cctcataggg aagacctgcg
    10561 aggcggcgcg agaagcaggg atctgggtcg gggtgtgcgg ggagtcggcc ggggagcccg
    10621 ccatgattcc caagctcgtc gagctcggcg tcaccgagct ctccatgagc gccccctcca
    10681 tcccaagggc aaagaagatc gtctccgagc tgtaagggtc gacgctccgg tcgcgcccga
    10741 cgcgggaggt ggggctccgc ggggtatggc cggcggcgcg ggtgggtgcc gcgcttaggc
    10801 gttgcgcggg acgaggaaac gtaagggtag gcaggaggag agcatgaagg agcaggaagc
    10861 aggccgtcgg acgggcggcg aggccgcgga ggcgagagcc gccggggggt tcgtatacgg
    10921 cttcacggag gatctggggt cggaagacgt cctggccctg tgcgggggga agggcagtgg
    10981 gctcatgcgt atgcgccggc tcggcctccc ggtgccggag ggcttcatca taacgacgga
    11041 ggcctgcgtt tcctacatag agtccggaga gttgccagaa ggtttgatgg ccgaggtgat
    11101 ggattacctg gcgcgtctgg aagaggagac gggccggggg ttcggcgacc ccgagaaccc
    11161 actgctagtc tcggtcagga gcggggcccc catctctatg cccgggatga tggacaccgt
    11221 cctgaacctc ggcttgggcg acgccaccgt tcgtggtctg gcgcggagca ccggtgatga
    11281 gcgcttcgcc caggattccc accggcgctt catccaggtg ttcggggaga tagtcctcaa
    11341 ggttcctggg cacctcttcg aggaggccat cgaggagctc aaacgcgagc ggggcgtcga
    11401 ggcggacacg gagctcacgg tccaggacct cggggagctc gtgggacggt tcaagagcat
    11461 agtcgcccgg gaggccaggg tggagttccc ggaggacccc cacaaacagc tagagctggc
    11521 gataggggcg gtcttcgact cgtggatggg agagcgggcg gcggcgtacc gcagggagta
    11581 cggcatcccg gagtctttgg ggacggccgt caccgtgcag cggatggtct tcgggaacat
    11641 gggagagacc tcggccaccg gggtggcgtt cacccgcaac ccgtccacgg gggagcaggg
    11701 catcttcggt gaattcttgc tcaacgccca gggtgaggac gtggtcgcgg gtatccgcac
    11761 accccttccg ctcagggaga tggagcgggt cctgcccaac gcctaccacc agttcctcga
    11821 gaccgccgag cggctcgagc gcgagaacca ggacatgcaa gacacggagt tcacggtcga
    11881 gcgcgacacg ctgtacatgc tccagaccag gagcggcaag cgcacggctg aggcggccct
    11941 caagatcgcg cgcgacatgg cggaggaggg tttgatctcc cgcgaggagg ccgtcatgcg
    12001 cgtcgagccc gcgtcgctcg accaactcct ccacccccgc gtggacccgg aggccgacct
    12061 cgaggtcctg gcggagggcc tccccgcctc ccccggggcg gccacgggga aaatagtcct
    12121 caccgcagag gaagccaggg agcgggcgga ggccggggag gacgtgctac tggtgcgccg
    12181 ggagacccac ccggacgacg tcgagggcat gttcgcggcc agaggcgtgc tcactgccct
    12241 gggaggcatg accagccacg cggcggtcgt cgcccggggg ataggcaagc ccgccgtgac
    12301 cgggtgtggc gccctcaaga tagaccccac gcgaggcgag atcagtctgg acggcacgac
    12361 gttgaaggat ggcgacgtcc tcaccataga cggggcggac ggtcgcgtca tccggggcgc
    12421 cgttccgctg gtccagcctc aactgggcga ggacttcgag accgtgctcc ggtgggccga
    12481 cgaggcgcgc ggcctggggg tgagggccaa cgccgatacg ccggtagacg cccggcgggc
    12541 cagggagctc ggggccgagg gcgtcgggct gtgccgcaca gagcacatgt tcatggaggg
    12601 cgaacggctc cggataatgc gggagatgat cgtctcggaa agcgacgagg ccctgggcga
    12661 ggccctggtc cagctggaac ctatgcagcg tgaggacttc gaggggatct tccgggccat
    12721 ggacgggttg cctgttaccg tgaggctcct ggacccgcct ttgcacgagt tcctccccga
    12781 atcgaaggac ctggcgaagg agatagccct gcgcgaggcg cgcggagaga gcgccgaaga
    12841 gcagagacgg caactgcggg tcgcggagag cctcgaggag aggaacccga tgctggggct
    12901 gcggggctgc aggctcgggc tcttgcgacc cgagatttat ttgatgcagg cgcgtgccat
    12961 agcccaggcc gcgagggccg tccgggaggc cgggggcgag ccggtagtcg agataatgat
    13021 ccccctcgtc ggcttccctt cggagctaag gaagatgcgg gacctgatcc tggaggaact
    13081 cgccggagaa agcgtgccca tcgggaccat gatcgagctg ccgagggcgt gcgcggtggc
    13141 cggggagata gctcgcgtcg ccgacttctt ctccttcggc accaacgacc tgacccagat
    13201 ggtctgcggc ataagccggg acgacgccga ggagaagttc ctctccgact acctgaagag
    13261 cggcatcctg caggagaatc ccttcgagac cctcgaccag gagggcgtgg gcgaactggt
    13321 ccacatggcc cgcgagcggg ggcgggagac gaacccgcac ctcaagctcg gggtctgcgg
    13381 agagcacggt ggcgatccca agagcgttgc cttcttccat gaggtcggcc ttgattacgt
    13441 ctcctgctcg ccttttcggg tgcccggcgc gcgcctcgcc gccgcgcaag ccgccctgaa
    13501 agagcgatcg accacgagac aaccagtctg aatgcgacgc acgatggtag cgtactgcgg
    13561 tccccagccc ccttgctgga gcgtggagtg ggaggtaggg gctgccgtga gtggaatatg
    13621 gatcgggtgg gcctgagatg atctccgccg tgctcttcga tatggatggc accctgctgg
    13681 agacggagga gctcaaagcc ctctcctacg cccgcgcagc cgtcgaactt cggcccgagc
    13741 acgtccgcga ggccgacgtg ctcgcggctt tcggcgacct ggtgggcctc tcgcgtgaag
    13801 aggtcgccag cgccctgatg cggcgcttcg ggttggaggg ggcggctcgg gagcgcatgc
    13861 atgagttcga agccgacgag ccgtggcagg ccttcgcggg ggtccggctc cggatctacg
    13921 agggattgct cggtgaccct gacctgctcc tgaaacagcg ctacccgcac aacatcgagc
    13981 tgctgcatga actgcggcgc caaggttatc cgacggcgct gacgacgatg tcccaccgcc
    14041 accacgtgcg gcgcgtgctc tccgtgctcg gcctcgccga cgccttcgac gtggtcgtgc
    14101 caagggatga cgtccggcgt ggcaagcccg acccggagat agatctgctc gcggcgagaa
    14161 agctgggtgt gccgccggag gagttcctgg tgatcgaaga ctcgcccgct ggcgtcaagg
    14221 cggcgctggc cgccgggatg gtggttatcg ccgtcgctac caccctcacc cgccgggggc
    14281 tccgcgagtc gggcgttctg gacccccaac gggtagtgga ggatccccgg acgctacccg
    14341 ccgtggtgcg ccggcggata gaggcggctg gaggaaaacg cgtgaaggag tagccagaga
    14401 gatccacgcc cggttctggc atgaggcgag aaggagagaa tatgggtttt cgtggtgcta
    14461 tcttcgatgt ggacggagtg ctggtcgact cgccgcacga gcaggcctgg cgcgaggggt
    14521 tgcaccagct gatggaaaac gagtggagcg acatccaggg ccagacgtct tacgctcccg
    14581 agaggttcac ctcggaggtc taccagcggg tcatgtccgg caagccgcgc tacgcgggcg
    14641 cgcaggccgc gctggagcac ttcggggtgc ccgacgccga gaggcgcgcc ctggagtacg
    14701 gcgaccgcaa gcaagtgatg atagtcgagc tgatcgaggc cggcgagttc cacgcctacc
    14761 cggacgcttt gcgctttgtc ctcgcggtca aggatgcggg gcttcacata gcggctgcct
    14821 cctcctcgaa gaacgcgaac gcattcctgg agaggataag gctggacgag ttcgtcgagg
    14881 aagagggcct gagctacggc tttgtggagc cgggctacac cctgctgggc atcctggacg
    14941 cgaacgtgag cggacgagac ttcgagcagg gcaagccgca tccggagatc ttcctgacgg
    15001 cggccgagga gctaggattc ccggcggaag agtgcttcgt cgtcgaggac gccgcgaacg
    15061 gggtgcaggc ggcgaaggca ggcaacatgg cggcgctggg gctggcgcgg gccgaggacg
    15121 aggacctgct caaagaggcg aacgcggatt tggtcgtcac gaccctcgat gacgtctccc
    15181 tcgatgcact cgccgaaggt cgcctagagc acgtgagcgt ccggtgaagg cgctcttgcg
    15241 gggggagtaa ggcgatggtt gctcgacaca ccgcggactt cagtgagtac caggacgccg
    15301 tggagatcgc gttggcgggg ctcaaggagg agcgggtggt cgagcggatc tgggccggtg
    15361 accacaccgt ctggggactg aagcctgagg agatagcgaa ccgtctgggc tggctcaaga
    15421 gcccgcaggt catgcgggag gagctggagg gaatctccgc actggtcgag gctgtgcgca
    15481 acgaaggcta cacccacgcc ctgctgctcg gcatgggtgg atccagcctg gccccggagg
    15541 tttatcgcaa cgtcttcggt gttgcggacg gacacctgga cctggccgtc ctagacagca
    15601 ccgaccccga cgccgtcctg gaacacgctg accggctcga cctttcgcgc accctgttcg
    15661 tcgtctccac caagtccggc gggacggtcg agacgttctc cttcttcagg tatttctaca
    15721 accgggtttc tgatacactc gatgaggatc gggccggcga acacttcgtc gcggtcaccg
    15781 acctcggcag cgggctgcag gacatcgctg agaagtaccg gttccgggcg acgtttctca
    15841 acgactcgaa catcggcggg cgctattcgg cgctctcgtt ctacgagctg gttccggcgg
    15901 cgctcatcgg cgtggacatc cgtaggctgc tcgacggcgc ggcagagatg gcgcgcgcct
    15961 gcggcaagag cggcgtcgag gagaaccccg gcgcgtggct cggggcggcg atgggggagc
    16021 ttgccaactc ggtcggacgc gacaagctca cgttggtcgg ggggccgacc ttcgctccgc
    16081 tcggggtctg gatcgagcag ctcgtagccg agagcaccgg taagaagggc aagggcatag
    16141 ttcccgtggg aagcgagtca cccggcgcgc cggaggtcta cggagacgac cggctcttcg
    16201 tctacagggg cgcggacggt agcgacggcg aagactctgc tggcaggctc gaggacctgg
    16261 cccaggccgg gaatcccgtg gtgcggatag aggcgcggga accctacgat ctgggcggtg
    16321 agatgttccg gtggatgatg gcgaccgccg tagccgggca catccttgcc atcaacccgt
    16381 tcgaccagcc gaacgtcgag tcggccaagg tgcttgccag gaagatggtg gcggagtacc
    16441 aggaacaggg tagtctgccc gggctacagc cgacgctgca agagcacagg atcaccgtct
    16501 acggctccgg cgaggcgaag acggctgcgg aggcgctgga agagttcctg tcgcgggctc
    16561 gtcccggaga ctacgtggcg cttcaggcct acgtaaaacc ctcggaggag acgaccagct
    16621 ccctccaagg gcttcgcctc cggctgcgag accggctgcg gctggcgacc acggtgggct
    16681 atgggccgcg gttcttacac tccacgggtc agttacacaa aggcgacgcc ggaaacgggc
    16741 tgttcgtcca gttcaccgcc gacgacgagc gggacgcgcc aattccggac gaggctggat
    16801 cggccgcctc gtccctcacc ttcggcgtac ttaaggaggc ccaggctctt ggcgaccgcc
    16861 aggcgctgct ggacgctggg cggagggtta tccgttttca ccttgacgaa gacgtgggcg
    16921 gcggtctggg ccggctgaca gcgtgggtat cgtgatgggc ccgccaccgg aggaaagctg
    16981 ggaatacgga gaggagcgat ccactcgact tagaccttag gaggacgcaa gatgcgaatc
    17041 gttcagttgt cggacatcca cgtgggaaca ccgcttttca ggcccgactt cttgaaagcg
    17101 gccatagagg agaccaacgc ctacgcgccg gacttcgtgg ccgtggccgg agacctcacc
    17161 accgcgggtt accggtggga gttcgaggag gccaagagct acctggacca gattgagtgt
    17221 gagaacgtgg tcgtgatcat gggcaaccac gatgccaaga acgtcggcta ccagcacttc
    17281 gaagacctgt tcggccagcg ggcacgttcg gtgacgtttt cggttcccga gggagaggtg
    17341 aagttggtgg cgcttgactc ctcaaagccg gacctggctg agggggaggt cggacgggag
    17401 aactacgggt ggattgacgc cgagttacga gactgggaga ggggggcgaa gatcgtgatg
    17461 gtccaccacc acctcctggc cgttcccggc accgggcgcg accggaacaa cttgcgtgac
    17521 tcaggggaca tgatggccgt gctgcgagac ctgaaagtgg acatggttct ttcggggcac
    17581 aagcatgtcc cctacgtctg gagcatctcc ggggtgaggg tgatccactc cgggaccgtc
    17641 tccagcatga gggtccgcgg aagcatagcc ccatcctaca acgtcatcga gttcgactcg
    17701 gagaaggtcc tggtcaatct ccggcatccg ggtgaaggag agcagccgtt cgcccgtttc
    17761 tcccgccagg cagtcacgac cagcgagatc ttcccggacc tcgaatactt catccgctac
    17821 gacaaagcgt ctgcatgaaa gagagaggag atgaccaaaa acatcagaga tctaccggac
    17881 gcggttgagc acagcgacga gctggcccaa cgcctggcgg gaagacggcc agccgtgttc
    17941 ctcgactacg acggcacgct gacgcccata gtggaccggc ccgaggacgc ggtcatctcc
    18001 gagagcatgc gcaacgccgt ccgcgaattg gccaaacgtt gccccgtgtg cgtggtcagc
    18061 gggcgcgata ggcgagtggt acaggagctc atgggcgtag acgacctgat cgtcgccggc
    18121 agccacggct tcgacatctg gagccccgcg ggcggtgaga tccagcgcga ggaaggcgag
    18181 gagttcgggg gactgctcga aggggtggag gcgcgtctgc gcgaggagtt gggcgggatc
    18241 gagggcgccc tcgtcgagcc gaagaagtcc tctgtggccg cccactaccg gctcgtttcg
    18301 gaggacgagc ggccaagggt gggacgggtc gtcgagaaga tcctcgccga acacccagac
    18361 gggctgaagg tgacgcccgg caagatggtc tacgaggtcc agccgaacat cgactgggac
    18421 aaggggaagg ccgttctgta tttgctcgga gcactcgacc tcgaccgcga cgacgtggtg
    18481 cccatctatc tgggcgacga cataaccgac gagcacgcat tcgaagcact ggcaacgaag
    18541 ggcatcggta tcttcgtcgg tcgcgccgac gatcccgagg ttgccgggcg aacgaccgca
    18601 gccgactacg tcctccacga cgtgccggag gtggaaagat tcttggatac gcttgcgcgc
    18661 tgacaaccgt cgataacgtc ttaaggagca agagacatgc tggaagagaa gagagagacc
    18721 acaggaacga ac
//
LOCUS       DASTKM010000243         3488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_961958, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000243 DASTKM010000000
VERSION     DASTKM010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961958"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3132)
                     /locus_tag="VFQ10_09990"
     CDS             complement(<1..3132)
                     /locus_tag="VFQ10_09990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9965715.1"
                     /translation="MNICTIIAKNYVAHARVLAESFLEHHPEETCYVLVIDDTEGYID
                     AASEPFELVRPAQLDIDDYEHMAAIYDVLELSTAVKPWLLRHLLDGRGLERVAYLDPD
                     IRVYDRLDEIERLTGEHGLVLIPHITSPLPRDGKRPSEADILISGTYNLGFISLEHGS
                     ATDGLLDWWCERLKTDCVVAPDTGYFVDQRWMDFVHGVMPDFFVLRDPSYNVAYWNLH
                     GRELTYYKGRYLVDGRPLRFFHFSGFDPDHRKRLSKHQTRIEIRRGSALARACNSYAD
                     ELLAHGYKRAKTWPYSYGVLPNGVRLDSGMHRLYRKGEEEGAPRQSIFTPEGADRFVA
                     WLNEPAPVGGEHGVTRYLYEIYSGRPDLRQEFPNLDGPDAMRLIEWHRVHGQVKLPYP
                     VHPSAEQDGHDEPQTSDTTVGVNVAGYFRAELGVGETARQVVAALERADMPVATVGLS
                     ASASRQEHEYETDTIRPTFPVNLICVNADMLPTFADQAGPGFFFDRYSIGLWWWEVNA
                     FPERFFKAFEPLDEIWVGSHFVADAISAVSPIPVVKVTMPVSMPEIEEFDRGDLRLPE
                     GFVFLFVFDYHSVFERKNPLALVEAFEQAFPEGSEASLVLKCINSEHHQGEHGRLIEA
                     AKGHRDIHVIDRYVTAAEKNAMFAGCDCYVSLHRSEGFGNTLAEAMYLGKPVIATGYS
                     GNMEFMTPQNSYPVEYTLRPVGDDAGPYPPDGEWADPDIRHAARLMRHVFEDQGEAGE
                     RGRRAADDLRRNHSAEAAGKAMAERIRRVRARLGAGPDAGRPLAYSPAGLDTNPLTHR
                     IATTPQPATSSSTLGRVLAPFRRVLLKLMWPFALQQRAVNEELLAMTKEADQSLRAVL
                     NQIESRYAKDRADMLSAVNHNREQARTQEERLLRLEKRLAGSDTPGPQMGSVSKQLED
                     AGPRAAGTGGFSRQAISRLITPFTAQQQKINDQLFTAVSTLGEKLGGLVPRVEAADRL
                     VAETRSLPYVSGSPFEIFEEPVAGRVQGYGRRNGDPERTGTYDAFEDIFRGPEHFIRD
                     RQRRYLDLLGNREP"
BASE COUNT          602 a         1038 c         1171 g          677 t
ORIGIN      
        1 gggctcgcgg ttcccgagga ggtcgaggta gcgacgctgg cggtcgcgga taaagtgctc
       61 ggggccccgg aagatatcct cgaacgcgtc gtaggttccc gtgcgctccg gatcaccgtt
      121 acggcggcca tagccctgga cacgccctgc cacaggctcc tcgaagatct cgaacgggga
      181 accggatacg taagggagag agcgtgtctc ggccacgagc ctatctgctg cctctacgcg
      241 gggaacgagc ccgcccagct tctcgccgag cgtactgacc gcggtgaaga gctggtcgtt
      301 gatcttctgc tgctgcgccg tgaacggggt aatgagacgg gagatcgcct gcctggagaa
      361 gccgccagta ccggccgctc tcgggccggc atcctctaat tgtttgctga cactacccat
      421 ctgtgggcct ggtgtgtcgc tcccggccaa ccgcttctcc aggcggagga gccgctcttc
      481 ctgtgtcctg gcctgctccc tgttgtggtt tacggcgctc agcatgtcgg cgcggtcctt
      541 cgcgtaacgt gactcgatct ggttgaggac ggcgcgcagg ctctgatccg cctcctttgt
      601 catggcgagg agctcttcgt tgaccgcccg ctgttgcagg gcgaagggcc acatcagctt
      661 cagcagaacc cgccgaaaag gggcgaggac cctgcccagg gtggagctcg aagtcgccgg
      721 ctgtggggtg gtcgcgatgc ggtgtgtaag cgggttggta tcgagtcccg ccgggctgta
      781 cgcgagtggc ctgcccgcgt caggtcctgc gccaagccgt gcccggacgc ggcggatgcg
      841 ctccgccatg gcctttcctg cggcctctgc ggagtggttt cggcggaggt catccgccgc
      901 cctccggccg cgctcgccgg cctccccctg gtcctcgaag acgtggcgca tcagccgtgc
      961 ggcgtgccgt atgtcgggat cggcccactc gccgtcggga gggtagggcc cggcatcgtc
     1021 gccgacgggc cttagcgtgt actcgacggg gtaactgttc tggggcgtca tgaactccat
     1081 gttgcccgag taccccgtgg cgatcaccgg tttgccgagg tacatggcct ctgcgagggt
     1141 gttgccgaag ccttccgagc ggtgaagcga gacgtagcag tcgcagccgg cgaacatcgc
     1201 gttcttctcg gccgccgtga cgtaacggtc tataacgtgg atgtccctgt ggcccttggc
     1261 ggcttcaatc agcctcccgt gctctccctg gtggtgttcg ctgttgatgc acttgagcac
     1321 gagggaggcc tccgagccct cggggaaagc ctgctcgaac gcctcgacga gggccagagg
     1381 gttcttgcgc tcgaagacgc tgtggtagtc gaagacgaac aggaagacga agccctcagg
     1441 gagccggagg tccccacggt cgaactcctc tatctccggc atagataccg gcatcgtcac
     1501 cttcaccacg ggtatcggcg agacggccga gatcgcatcg gccacgaagt gcgagccgac
     1561 ccagatctca tccagtggct cgaaggcctt aaagaagcgc tccgggaacg cgtttacctc
     1621 ccaccaccac agcccgattg agtagcggtc gaagaagaac cctggcccgg cctggtccgc
     1681 gaacgtgggg agcatgtcgg cgtttacgca gatgagattg accgggaagg ttggcctgat
     1741 cgtgtcggtc tcgtactcgt gctcttggcg gctagcagag gcggagaggc cgaccgtggc
     1801 aacgggcatg tccgccctct ccagtgccgc gaccacctgc cttgccgtct ccccgacgcc
     1861 gagctcggcg cggaagtacc cggcgacgtt gaccccgaca gtggtatccg atgtctgcgg
     1921 ctcatcgtgg ccatcctgtt cggcggaggg gtgcacgggg taaggcagct tcacctgccc
     1981 gtggacgcgg tgccactcga tgaggcgcat cgcgtctgga ccgtcgaggt tggggaactc
     2041 ctgcctgagg tcgggacggc ctgagtagat ctcgtagagg tagcgtgtaa cgccatgctc
     2101 cccacctacg ggggccggct cgttgagcca ggcgacgaac ctgtcggcgc cttctggagt
     2161 gaagatggac tgtcgcgggg ctccttcctc ctcgcccttg cgatagagcc tgtgcatgcc
     2221 agagtcgagc cttacgccgt tcggcagtac accgtaggag tagggccagg tcttcgccct
     2281 tttgtagccg tgggcgagca actcgtccgc gtaagaatta caggcgcggg ccagcgcgga
     2341 gccgcgccgg atctcgatgc gggtctggtg cttgctgagc cgctttctgt ggtcgggatc
     2401 gaagccggaa aagtggaaga aacgcagggg tcgcccgtcc acgaggtagc ggcctttgta
     2461 gtaggtcagt tctctgccgt gcaggttcca gtaggccacg ttgtaggagg ggtcgcgcag
     2521 gacgaagaag tcgggcatga cgccgtgcac gaaatccatc caccgctggt cgacgaagta
     2581 gccggtgtcg ggggcgacca cacagtccgt cttcaggcgc tcgcaccacc agtcgagcag
     2641 gccgtccgtg gctgacccgt gctccaggga gataaagccc aggttgtagg tgcccgagat
     2701 caggatgtcg gcttcggagg gccttttgcc gtcgcggggt aggggggagg tgatgtgggg
     2761 gatgaggacg agcccgtgct cgccggtcag cctctctatc tcgtccagcc tgtcatagac
     2821 tcgtatatcc gggtcgaggt aggcgacccg ctccagaccg cgcccgtcga gcaggtgccg
     2881 cagcagccac ggtttcacgg cggtcgagag ctcgagcaca tcgtagatag ccgccatgtg
     2941 ctcgtagtcg tcgatatcta gctgggccgg tcttactagc tcgaacggtt cggaggcagc
     3001 gtctatgtag ccttctgtgt cgtctatgac gagcacgtag caagtctctt caggatgatg
     3061 ttccaagaag gactccgcca gtaccctcgc gtgggcgaca tagttcttgg cgatgatcgt
     3121 gcagatattc atagccggaa ttgaatcata ctcagcctgg cacttcgcaa aacagccagg
     3181 aacctgatac ggttctgcta ttaatcagtg aaccttcaag gtgtagaata gcttcgtgga
     3241 tgccgttgag tagagcgcct aacagtttga agacgcacag gaaatggtcc ttcacgatct
     3301 gagacgatac caagattctg ggggagatga gtgatcgaag cacgaaaccg ggtcaagtgg
     3361 aagaacaggg aggggatccc cgagtacgcg gtccgctgga actgggtgga ggattcctca
     3421 gactcggggt tcaccgcggt cttgagggtc aaggacgaag cccgttcgct gccctgggtg
     3481 ctgcctgg
//
LOCUS       DASTKM010000244         5909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_609099, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000244 DASTKM010000000
VERSION     DASTKM010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609099"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1445)
                     /locus_tag="VFQ10_09995"
     CDS             complement(42..1445)
                     /locus_tag="VFQ10_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965716.1"
                     /translation="MTTRQDAEPSPWGLLWGVFWRSVLWYTVAGAVLGGMYGCAIIAV
                     AMLSTDASEAIEPSEVWAMRAIVAFFGVTGFIAGWALARAIGREMRPLLGAVIGGMAG
                     VQLGGFHYLFAYFYAFPFGTVLGGAYGLAVGFVNAILVAALTRALFFPLSEPRLYRRA
                     VVWTSLLGVLILPGFWFVILIRSSGYTEVGGMTGTFWGDVFIYAGFPPLILALVAQWF
                     GGRLATWYGIVAPRTPATTEVPALRWTLRREKPRRIAYAALALTVLVLLAAGFRLYQT
                     RTILTRIPSREAILSRDGERAAVLAPGEFEVYSVPEGRRRHALPLGREEAYDLDAAVW
                     ASNGRFLATSDGGRVHVYDAVEKAEVAQLPVEAAAQLAWSPDGSLLAVSTLEGTDNPF
                     AAESEARIWSRDSRSWTHTLPVGEVWSDPEDSTLAFDANGDTFAATATRRHGPSSRRW
                     RRRSGGCCGRRSGGRYA"
     gene            complement(1445..2161)
                     /locus_tag="VFQ10_10000"
     CDS             complement(1445..2161)
                     /locus_tag="VFQ10_10000"
                     /inference="COORDINATES: protein motif:HMM:NF036524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9965717.1"
                     /translation="MEETLRRALTPKARRTRERILEAALALFADRGYEATTMRDVARE
                     AGASLGLAYRYFASKEEFALALYIRLAEESEEWARDGLVGGTVAERFEAAMLAKLDQV
                     SPHRGSLAALLTRALDPNSRLSTLGEGTAAVREKMVGVFLEVVRGASDAPGEKQAMQL
                     GSVLYALHLAIILYWFHDRTPEARATRELVGSARETLRYLRPAFRLPPMSRVLSRLAG
                     ALNDIGMNTTGPSRGEPRKA"
     gene            complement(2550..3290)
                     /locus_tag="VFQ10_10005"
     CDS             complement(2550..3290)
                     /locus_tag="VFQ10_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9965718.1"
                     /translation="MDNPETPSWFRSKAVAVHLYTASGAVLALLMLVAAFQGEAVKAL
                     WLMLASLLIDSTDGLLARRFRVSEALPFFDGVLLDNIVDYMTYVFAPVVLLWSEGYLP
                     DGNAGIVLAALPLLASSYQFCRVDAKTEDHYFLGFPSYFNVVAFYAIVFHPSPLVLAA
                     VLVACSFLVFVPIRYVYPTRTVAFRKLSLTLTAMWLASYAAILWQMPDPDPLILTFSI
                     LYLVYYFGLSLYLSGKLLEARRTQEREA"
     gene            complement(3377..5068)
                     /locus_tag="VFQ10_10010"
     CDS             complement(3377..5068)
                     /locus_tag="VFQ10_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HET9965719.1"
                     /translation="MQRLGETLDRIDRKGYGAYRDLSGTHDLGHFVLFMDRVQRDPFA
                     PPSLIRIQTKVNPFDPTLFGNSVRRVAFEDFLTRSVEREIRRVVRGNRGSGGSGRVEI
                     QRASQVVLPRTSMVVEPGYVEARMAVGLPARGRSVDASAARTVLLEELPEVVRRGLDP
                     APEGGVDVERAKLHVESVEDADHLRGLLPGLGLVAFVADGAVLPRESGASDRPLREGA
                     VPFGSPEEHRVEVELPNKGVVSGMGVAEGVTLVVGGGFHGKSTLLSALSWGVYDHVPG
                     DGRELVVTRGDAVKVRSEDGRSVSGVDISAMIGDLPGGRSTKVFSTPNASGSTSQAAN
                     IAEAIEVGTSLLLVDEDTSATNFMIRDERMRELVRREPISPYIDLVRPLHRSLGVSTV
                     VVVGGVGDYLDVADRVILLEDYAPSDATPRSREVTKRFPLRAPLTQRAVRPPRERAID
                     TSSINLRRGKRQTARGRGLHTIELGRERVDLSYLEQLAEAGQTEAVARIIREWATAGE
                     VRGVGELVREALASISEKGLDSLGNYRGHPGEMSLPRAQEVAAATNRVRPLRAAP"
     gene            complement(5073..>5909)
                     /gene="trpS"
                     /locus_tag="VFQ10_10015"
     CDS             complement(5073..>5909)
                     /gene="trpS"
                     /locus_tag="VFQ10_10015"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET9965720.1"
                     /translation="DPKVLRQKIREVAAIYLASGLDPERCVIFRQSKVSGHAELCWLL
                     NSVARFGELSRMTQFKDKTRKGGVESASAGLFDYPVLQAADVLLYNAHLVPVGEDQRQ
                     HLELMRTLARRFNHLYGETFVVPEAMIPPTGAKVMALDAPEEKMSKSSPRPGSYVAIL
                     DDPDSIRKKIRRAKTDSGTEILASQDKPAITNLLDVYSAMSGEPVSGIEADYVGKGYG
                     DFKKDLAEVVVEGLAPVRDRAYELLDDPAELDRLLDSGARRAQEAALPNLESARARLG
                     LD"
BASE COUNT         1063 a         1972 c         1860 g         1014 t
ORIGIN      
        1 cgtaatcctg aatatcttgc tgccccgcca gagacggagc atcaggcgta acgcccgccc
       61 gagcgtcgac cgcagcatcc accagagcgc cttctccacc gccgactcga aggcccatgc
      121 cttctcgtcg cggtcgccgc gaaggtgtcg ccgtttgcat cgaaggccag ggtggaatct
      181 tcggggtcgg accacacttc tcccacgggg agggtgtggg tccaactcct gctatccctg
      241 ctccatatgc gcgcctcgct ctcggcggca aacggattat ccgttccctc cagggtcgag
      301 acggccagca aagatccatc cgggctccag gcaagttggg cggcggcctc gacgggcaac
      361 tgcgcgacct ccgctttctc caccgcgtcg tagacgtgta cgcgcccgcc gtctgatgtc
      421 gccaggaacc tgccgttgga ggcccacacg gccgcgtcca gatcgtaagc ttcctcccgg
      481 ccgagcggca gcgcgtgccg tctccggcct tccgggacgc tgtagacctc aaactctccg
      541 ggagcgagaa ccgctgccct ttccccgtcc cggctgagga tggcttccct gctcgggatg
      601 cgcgtcagga tcgtgcgcgt ctggtacagt ctgaagcccg ccgcgagcag cacgagcacc
      661 gtgagcgcga gcgccgcgta cgcaatccgc cggggcttct cgcggcgcag cgtccaccgg
      721 agcgccggca cctctgtcgt tgctggcgtc ctgggagcta ctatcccgta ccaggttgcc
      781 aggcgtccgc cgaaccactg ggccacgaga gcgagtatca gcggcggaaa gcccgcgtaa
      841 atgaatacgt cgccccagaa agtgccagtc atgccgccga cctccgtgta tcccgaagat
      901 cgtatgagga tcacgaacca gaaccccggc aggatcagca ctccgagcag gctcgtccac
      961 acgaccgccc gccggtaaag tcggggttcc gatagcggga agaagagcgc ccgcgtaaga
     1021 gccgccacca ggatagcgtt tacgaaccct accgccagac cgtacgctcc cccgaggacc
     1081 gtgccgaaag ggaacgcata gaagtaagcg aacaagtagt ggaatccacc cagttggaca
     1141 ccggccatgc ctcctatcac ggcgccgagc aaaggccgca tctcccgccc gatagcgcgc
     1201 gccagcgccc aacccgcgat gaaccccgtc acaccaaaga acgccactat cgcgcgcatg
     1261 gcccacactt cggagggctc tatggcctcg ctcgcatcgg tcgacaacat ggcgaccgcg
     1321 atgatcgcgc acccgtacat cccgcctagc accgcccccg ccaccgtgta ccacagcacg
     1381 ctgcgccaga agacgcccca gagcaacccc catggtgacg gttcggcgtc ttgccgcgtg
     1441 gtcatcacgc cttcctgggt tcgcctctgg acgggccggt ggtgttcatg ccgatgtcgt
     1501 tcagcgcacc ggcgagccgg gagaggacgc gggacatcgg tgggagccgg aacgccggcc
     1561 gcaggtaccg gagcgtctcg cgggcggagc caacgagctc gcgcgtagca cgggcctcag
     1621 gcgtcctgtc gtgaaaccag tagaggatga tggcgaggtg caaagcgtag aggacgctcc
     1681 ccagttgcat tgcctgcttc tccccggggg cgtcgctcgc gccccgcacg acctcaagga
     1741 agacaccaac catcttctcg cgcaccgcag ctgtgccttc gccgagagtc gagagccggg
     1801 agttggggtc gagcgcgcgg gtgaggaggg cggccaggga accgcgatgg ggggatacct
     1861 gatccaactt ggcgagcatc gctgcctcga accgctccgc caccgttcct ccgaccagtc
     1921 catcccgcgc ccactcctcg gactcctcag ccaaccgtat gtacagcgcc agcgcgaact
     1981 cttccttgga cgcgaagtac ctgtacgcga gccccaggct ggcccccgcc tcccgcgcta
     2041 cgtcccgcat cgtcgtcgcc tcgtatcccc tgtcggcgaa cagcgccagc gcagcctcca
     2101 gaatccgctc cctcgtgcgc cgtgccttcg gcgtcaacgc ccgcctcaac gtttcctcca
     2161 tctcatcctt ccataaaaat gaacacgttc atttcctgaa tattagggta ttggcaggag
     2221 gctgtcaagc ctggaggccg attcttcgaa agcagcgaag tcttcgaccc ggattcgaga
     2281 ccgggcgggt ctcgtgggtc gagggactgg tacgtaaact ggcacaccgt ctatggttgg
     2341 aggcgcgtag cgtccgtcgc cgtatattcc cgctttcgcg ggaatgacgg tggtgtggga
     2401 gcaggctggt tagaagtggc gcaaccggct aggagcaatc agcgagagag aaagctgaga
     2461 agctgacgag cgaggccgaa gcgtaccgac gagcaagggc acaagcccga gcgtgccgac
     2521 aagcgtaggc cgcgggccgg agcgtgcttc taggcttcgc gctcctgggt gcggcgggct
     2581 tcgagcagct tgcccgagag gtagaggctg aggccgaagt aatagacgag gtagaggatg
     2641 gagaaggtca aaataagggg gtcggggtcg ggcatctgcc agagtatcgc tgcgtagctg
     2701 gcgagccaca tagccgtgag cgttagtgag agcttgcgga aggcgactgt gcgcgtcggg
     2761 tagacgtagc ggatcgggac gaagaccaag aacgagcagg ccacgagcac ggcggcgagc
     2821 accagcgggc tgggatggaa gacgatggcg tagaaagcga cgacgttgaa gtagctaggg
     2881 aagccgagga agtagtggtc ttcggtcttg gcgtcaacgc ggcagaactg gtagctggag
     2941 gccaggagcg gcagcgcggc gagaactatc cccgcgttgc cgtcgggcag gtatccttcg
     3001 ctccagagca ggaccacggg ggcgaagacg tacgtcatgt agtcaacgat gttgtcgagg
     3061 agcaccccgt cgaagaaggg gagcgcctcg ctgaccctga agcggcgggc gaggagaccg
     3121 tcggtagagt cgatgaggag cgaagcgagc atcagccata gcgcctttac ggcctcaccc
     3181 tgaaaagcgg ccacgagcat aaggagtgcg agcaccgcgc cgctcgccgt gtacaggtgc
     3241 acggccacgg ccttcgatct gaaccaggag ggcgtctccg ggttgtccac gtcagcagct
     3301 cctctatatg atgttccctg atctcctcca cgctcagtac ctaatgatac cagccagagc
     3361 agtttatctt ctacgtctat ggggccgccc gcaacggacg tacgcggttt gtggcggcag
     3421 cgacttcctg ggcgcgcggc aggctcatct caccagggtg tcccctgtag ttacccagag
     3481 agtcgaggcc tttctctgag atggaggcta gcgcctctct gacaagttcc cctactccgc
     3541 ggacctcgcc tgcggtggcc cactcccgga taatacgggc cactgcctcg gtctgtccgg
     3601 cctctgcgag ttgctccagg taggagaggt cgacccgctc gcgccccagc tcgatagtgt
     3661 ggaggcctcg gccacgggcg gtctgtcgtt tgccccgtct caggtttatc gaggacgtgt
     3721 cgatggcgcg ctcccgcggt ggacgcaccg ctctttgcgt gagcggtgcg cggagcggga
     3781 accgcttggt tacctcgcgg gagcgggggg ttgcgtcgga gggcgcgtag tcttcgagca
     3841 ggatcacccg gtcggctacg tcgaggtagt ctccgactcc cccgacgacc acgaccgtcg
     3901 agacgccaag agagcggtgc aaggggcgta ccaggtctat atacggcgag atgggctcgc
     3961 gcctcaccag ctcgcgcatc ctctcgtccc ggatcatgaa gttcgtcgcg ctcgtgtcct
     4021 cgtccacgag caggagcgac gttcctacct cgatggcctc ggcgatgttc gcagcctgcg
     4081 aggtcgagcc tgaggcgttc ggggtcgaga agacctttgt gctccggccg cctggcaggt
     4141 ccccgatcat ggccgagatg tccaccccgg agacgctcct gccgtcctcg gacctgactt
     4201 tgaccgcgtc tccccgcgtc acaacgagct ccctgccgtc ccctggcacg tggtcgtaaa
     4261 cgccccacga gagcgccgag agcagcgtgc tcttcccgtg gaaccctccc ccgaccacga
     4321 gcgtgacgcc ctcagcaacc cccatccccg aaacgacacc cttgttcggc agttccacct
     4381 ccaccctgtg ctcctccgga ctcccgaagg gtacggcacc ttctcttagc ggtctgtcgc
     4441 tcgctccgct ctctctcggt aacaccgccc catcagcgac gaacgcgacc agacccaacc
     4501 cgggaagcag cccccgcaag tgatcggcgt cctcgaccga ctcgacgtgg agcttcgccc
     4561 tctctacgtc tacgccgccc tcgggggcag gatcgagtcc ccgacgcacg acctcgggca
     4621 gctcttcaag cagcaccgtg cgggctgcgc tggcgtcgac gctcctgccc cgtgcgggga
     4681 ggcctacggc catgcgggcc tcgacgtatc cgggctcgac gaccatcgag gtgcggggga
     4741 ggacgacttg ggaggcccgc tgtatctcga cccgtccgct cccacccgag ccccggtttc
     4801 cgcgcacgac gcgccggatc tccctctcga cgctcctggt aaggaagtcc tcgaaggcga
     4861 ccctgcgcac cgaattcccg aagagcgtag gatcgaaagg gttcactttg gtttggatcc
     4921 ggatcaggga aggcggagcg aagggatcac gctgcaccct gtccataaac aggacgaaat
     4981 gacctaagtc gtgagtgcct gaaagatctc tgtaggctcc gtatcccttg cgatctatgc
     5041 ggtcgagcgt ttccccgagg cgttgcattc agctaatcga ggcctagcct cgccctggcg
     5101 ctctcgaggt taggcagcgc ggcttcctgc gcccgcctcg cgcctgagtc gagcaggcgg
     5161 tcgagctcgg ccgggtcgtc gagcaactcg taggcacggt ccctgacggg ggcgagtccc
     5221 tctacgacga cctcggcgag atccttcttg aaatcgccgt agcccttgcc tacgtagtcg
     5281 gcttcgatgc cggatacggg ctcgcccgac atcgccgaat agacgtctag taaattggta
     5341 atggccggct tgtcctggga ggccaggatc tccgtccccg agtccgtctt ggcccgtcgg
     5401 atcttcttcc ttatagagtc cgggtcgtcg aggatggcga cgtaactgcc cggcctcggg
     5461 gaactcttgc tcatcttctc ctcgggcgcg tcgagcgcca tcaccttcgc cccggtaggc
     5521 gggatcatgg cctcaggcac gacgaaggtc tccccgtaga ggtggttgaa cctcctggcg
     5581 agcgtccgca tgagctcgag gtgctgacgc tggtcctcgc ccacggggac caggtgcgcg
     5641 ttgtaaagga gcacgtcagc ggcctggagc acggggtagt cgaagagacc cgcgctcgcc
     5701 gactcgaccc cgcccttcct cgtcttgtcc ttgaactgcg tcatgcgcga gagctcccca
     5761 aaacgcgcca cactgttgag cagccagcaa agctccgcat gcccgctgac cttgctctgg
     5821 cggaagatca cacaccgctc agggtcgagg ccagaggcga ggtatatggc cgctacctcc
     5881 ctgatcttct gcctgagcac cttcgggtc
//
LOCUS       DASTKM010000245         6846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_787201, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000245 DASTKM010000000
VERSION     DASTKM010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787201"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..171)
                     /gene="rpmJ"
                     /locus_tag="VFQ10_10020"
     CDS             complement(58..171)
                     /gene="rpmJ"
                     /locus_tag="VFQ10_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET9965721.1"
                     /translation="MKVRASVKPICERCKVIRRRGVVRVICTNPRHKQRQG"
     gene            complement(194..415)
                     /gene="infA"
                     /locus_tag="VFQ10_10025"
     CDS             complement(194..415)
                     /gene="infA"
                     /locus_tag="VFQ10_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET9965722.1"
                     /translation="MAKEDVIEVEGTVTEALPNTQFRVELENGHNVLAHISGKMRMNY
                     IRILPGDRVKVELSPYDLSRGRITYRFRT"
     gene            complement(505..1269)
                     /gene="map"
                     /locus_tag="VFQ10_10030"
     CDS             complement(505..1269)
                     /gene="map"
                     /locus_tag="VFQ10_10030"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET9965723.1"
                     /translation="MIVRKSKSELEAMREGGRITAACLKMLEENVRPGVTTRELDRLA
                     EEFIRDRGGKPEFKGYQGFPASICASPNAMIVHGIPGSYRLKNGDIISLDVGVRYEGF
                     VTDSATTVPVGHVHEDVVGLLGTTRECLAAATNQMRAGNRLGDVGHAIQALAESRGYG
                     VIRDLVSHGVGRQMHEDPQIPNYGSPGTGPRLLPGMTFAIEPMITLGDYDIRLSEWDG
                     WSIYTADGSLSAHFENTVAVTENGPLVLTQNGGSGG"
     gene            complement(1266..1898)
                     /locus_tag="VFQ10_10035"
     CDS             complement(1266..1898)
                     /locus_tag="VFQ10_10035"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET9965724.1"
                     /translation="MKLILLGPQGAGKGTQAQRLADEVGATHISTGDIVRAEIKSGSE
                     LGKKVQEYNDRGELVPNEIIVEMAKPYLDDADSWILDGFPRNEAQAKALDQALQDIGE
                     DLDAVVALEAPDDVLVDRLSGRRQSQATGEVYHVEYDPPPEDDSGPFVQRDDDAEKAI
                     RRRLQIYHDQTEPLKDYYADRDLLITVDAEQEIPKVTKDILGAVGREEDA"
     gene            complement(1895..3130)
                     /gene="secY"
                     /locus_tag="VFQ10_10040"
     CDS             complement(1895..3130)
                     /gene="secY"
                     /locus_tag="VFQ10_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET9965725.1"
                     /translation="MKLLFTFAMLAAYRLGAYIPLPGVDPSVLGGGLQSSNPITGLFG
                     TFTGGAFNNFSVFSLGIMPYITAAIVMQLMTVAIPRLQELAKEGETGQQKITQYTRYF
                     TLALALIQSVAMVLFFRSAQGGGALAGTSPIEILVAIVTLTAGVMLTMWLGELISQRG
                     LGNGISLIITASILSQAPVAITALRENGDVLTVVILGLLAVMIVAAIVFVNEGQRRIP
                     ITYAKRQVGRRMSQGGTTYLPLKVNMAGVIPIIFASSLLLFPVVLAQLSAGGDPDSIL
                     ARLSAFFDPGNAPYLILYALLIIMFTYFYTAVQFNPVEHADNLKKSGGYVPGYRPGQP
                     TAIYLNNVLTRITLFGALFLAVVAVLPYFISGALNLGNSIFLGGTSMLIVVGVSLDTV
                     RQLESQLMMRNYEGFLKKR"
     gene            complement(3178..3639)
                     /gene="rplO"
                     /locus_tag="VFQ10_10045"
     CDS             complement(3178..3639)
                     /gene="rplO"
                     /locus_tag="VFQ10_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET9965726.1"
                     /translation="MRLNEIKGAPGSKKAPKRVGRGTGSGRGKTSGRGHKGAGARSGV
                     SAFAAYEGGQMPLQRRLPRRKGEARGRHTVARPTVYAPVNLGELQGLPGDTIGPDELR
                     AAGLVRKKAELIKILGQGDAGRAVTVKAHAFSKAAREKIEAGGGSVEVLEK"
     gene            complement(3642..3824)
                     /gene="rpmD"
                     /locus_tag="VFQ10_10050"
     CDS             complement(3642..3824)
                     /gene="rpmD"
                     /locus_tag="VFQ10_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET9965727.1"
                     /translation="MSPLKVTQVRSVVGSKQRHKRTVRALGLKRIRDSRVHEDTPQIR
                     GMVHKVQHLVHAEEVD"
     gene            complement(3821..4354)
                     /gene="rpsE"
                     /locus_tag="VFQ10_10055"
     CDS             complement(3821..4354)
                     /gene="rpsE"
                     /locus_tag="VFQ10_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET9965728.1"
                     /translation="MGRPRSDNRQGGGRGGRQRDGASDLQDRVVEIRRVAKVVKGGRR
                     FNFSALVVVGDGKGRVGTGLGKANTVPAAIQKGQERAKRNMFDVPMRNTTIPHEVTGK
                     FESSSVLLKPASEGTGVIAGGGVRAVLELAGIKDVLTKALGSTTSVNLVQATVEGLQR
                     LRSKADIEQTRGVKITL"
     gene            complement(4366..4716)
                     /gene="rplR"
                     /locus_tag="VFQ10_10060"
     CDS             complement(4366..4716)
                     /gene="rplR"
                     /locus_tag="VFQ10_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET9965729.1"
                     /translation="MAVLEKRMHREKRHKRVRRKVAGTASRPRLSVYRSNVHIYAQLI
                     DDDAGKTLAAADSRQVGESENRKDAARKVGALIAKEASQAGIEAVVFDRGPNKYHGRI
                     AALAEGARQGGLTL"
     gene            complement(4731..5264)
                     /gene="rplF"
                     /locus_tag="VFQ10_10065"
     CDS             complement(4731..5264)
                     /gene="rplF"
                     /locus_tag="VFQ10_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET9965730.1"
                     /translation="MSRIGKAPVEIPGAVSVELSGRSVKVNGPKGELTVPVARGIDVR
                     QEDGNVIVERAADTPEHRAMHGLTRSLIYNAVTGVTEGFTKTLNIAGVGYRAALQGSD
                     INLQVGYSHPVSISPREGIEFEVPNATTIIVRGIDKQRVGQTAAEIRMIRPPEPYKGK
                     GIRYSDEQIRRKVGKAG"
     gene            complement(5275..5673)
                     /gene="rpsH"
                     /locus_tag="VFQ10_10070"
     CDS             complement(5275..5673)
                     /gene="rpsH"
                     /locus_tag="VFQ10_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET9965731.1"
                     /translation="MSVNDPIADFLTRIRNAHMAKQETVEIPHSKMKAEISRILLEEG
                     YVQDVSERGSGVERRLVITLKYGPDGSRGIAGLRRMSRPGRRVYRKQTNIPRVLDGLG
                     VAILSTSQGILTDHQARRRGVGGEVLCFVY"
     gene            complement(5679..5864)
                     /locus_tag="VFQ10_10075"
     CDS             complement(5679..5864)
                     /locus_tag="VFQ10_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET9965732.1"
                     /translation="MTRKALLVKQQKKQKYAVREYNRCQRCGRAHGYYRKFGLCRICL
                     RELAHEGKIPGVTKASW"
     gene            complement(5875..6417)
                     /gene="rplE"
                     /locus_tag="VFQ10_10080"
     CDS             complement(5875..6417)
                     /gene="rplE"
                     /locus_tag="VFQ10_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET9965733.1"
                     /translation="MARLKERYHDEVVPDLEERFGIENSMRMPGLEKIVVNMGVGEAV
                     VNSRALDGAMEDLARITGQKAQLRRARKSIAGFKIREGMPVGARVTLRGERMWEFLDR
                     FISIALPRVRDFRGVSPASFDGRGNYAIGLKEQVIFPEISYDSIDATRGLDVAIVTTT
                     DSDEEARELLRLLGMPFRQS"
     gene            complement(6410..6727)
                     /gene="rplX"
                     /locus_tag="VFQ10_10085"
     CDS             complement(6410..6727)
                     /gene="rplX"
                     /locus_tag="VFQ10_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET9965734.1"
                     /translation="MGLKLKRGDTVVVISGKEKGKRGEIERVIPKENRVVVGGVNVRT
                     RHARPSQQNQQGLYHFEAPIDVSNVMLIDPNSGEPTRVGYRFTDSGEKLRVGKKSGKD
                     IDG"
     gene            complement(6730..>6846)
                     /locus_tag="VFQ10_10090"
     CDS             complement(6730..>6846)
                     /locus_tag="VFQ10_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HET9965735.1"
                     /translation="VIINPDGAPRGTRIFGPVARELREKGYTRIISLAPEVL"
BASE COUNT         1111 a         2291 c         2034 g         1410 t
ORIGIN      
        1 aggcctacct cgaccctctt ctccctgggg aggtctactc ctgcaatccg cgccatgcta
       61 accctgcctc tgcttgtggc gagggttcgt gcagatgaca cgcaccaccc cgcgacgcct
      121 tatgaccttg caccgctcgc atatcggttt cacgctcgcc ctgactttca caactctctc
      181 ctctaacgtc ttcttatgtc ctgaacctgt aagttatccg tccccggctc aagtcgtagg
      241 ggctcaactc caccttaacc ctgtcgcccg gcagtatccg gatgtagttc atccgcatct
      301 tgcccgagat gtgcgccagc acgttgtgcc cgttctccaa ctcgacccgg aactgcgtgt
      361 tgggcagcgc ctcggttacg gtgccttcaa cctctatgac gtcttctttg gccaaggacc
      421 tcctcaagta gttacaagat ggctgaagcc aaacaaatat cttaccacgc ccgagaggac
      481 gctcccaccg acgaacgcct ctcgctatcc gccggagccc ccgttctgcg tcaacaccag
      541 ggggccgttc tcggtaacgg cgacggtgtt ctcgaagtgg gcggagaggg agccatcggc
      601 cgtgtagatg gaccacccgt cccactcgct gaggcggata tcgtagtcgc cgagcgtgat
      661 catgggctct atggcgaagg tcatcccagg aagcaaacgt ggacccgtac ccggactgcc
      721 gtagttcggg atctgggggt cctcgtgcat ctgccggccg accccgtggg agaccaggtc
      781 ccttatgacc ccgtaaccac gcgactcggc gagggcctgt atggcgtggc ccacgtcgcc
      841 aagcctgttg ccagcccgca tctgattcgt cgccgcggcg agacactccc tcgtggtgcc
      901 gagcagcccg acgacatcct cgtgcacgtg ccccacaggg acggtggtcg ccgagtctgt
      961 tacgaaaccc tcgtagcgga cgccgacgtc gagggagata atatcgccgt tcttcaggcg
     1021 gtaggagcct gggatgccgt ggacgatcat ggcgtttggc gaggcgcaga tcgaagccgg
     1081 gaacccctga taccccttga actcgggctt gcccccgcgg tcgcggatga attcctcggc
     1141 gagccggtcg agctcccgcg tcgtcacgcc tggcctgacg ttctcctcaa gcatcttcag
     1201 gcaggccgcg gttatcctcc cgccctcacg catcgcctcg agctccgact tgctcttgcg
     1261 gacgatcaag cgtcttcctc tctgcctacg gccccgagga tgtccttggt gactttcggg
     1321 atctcctgct cggcgtcgac cgtgatgagg aggtcgcggt cggcgtagta gtccttcaag
     1381 ggctcggtct ggtcgtggta gatctgtagc ctgcgcctga tggctttctc ggcgtcgtca
     1441 tcgcgctgga cgaaagggcc cgagtcatcc tcgggcggcg gatcatactc gacgtggtat
     1501 acctcacccg tagcctggct ctggcgcctt cccgagagcc tatctacgag cacgtcatcg
     1561 ggcgcttcga gggcgaccac ggcgtcgaga tcctccccga tgtcttggag ggcctgatcc
     1621 agggccttgg cctgggcctc gttgcggggg aagccgtcga ggatccagga gtcggcgtcg
     1681 tccagataag gcttagccat ctccacgatg atctcgttcg gcaccagctc gcccctgtcg
     1741 ttgtattcct gcaccttctt gcccagctcg gagcccgact tgatctcggc gcgcactatg
     1801 tccccggtgg agatgtgcgt ggcgcccacc tcgtccgcga ggcgctgcgc ctgcgttccc
     1861 ttgcccgctc cctggggccc aagcaggatg agcttcatcg cttcttcagg aacccttcat
     1921 agttgcgcat catcaactgg ctctccagct ggcgtacggt gtccagtgag acgccgacca
     1981 cgatcagcat cgaggtgccg ccaaggaaga tggagttccc gaggttcaga gcgcccgaga
     2041 tgaagtaagg cagcacggcg accacggcca gaaacagggc gccgaagagc gtgatcctgg
     2101 tcagcacatt attcaggtag atggccgtcg gctggcccgg tctgtagcca gggacgtacc
     2161 caccactctt cttcaggttg tcggcgtgct ccacgggatt gaactgcacc gccgtgtaga
     2221 aataggtaaa catgatgatg agcagcgcgt agaggatcag gtaaggcgcg ttcccaggat
     2281 cgaagaaggc cgagaggcgg gcgaggatcg agtccgggtc tccccccgcg gaaagttgcg
     2341 ccagcacgac ggggaagagc aacagcgagg aggcgaagat tatgggaata acccctgcca
     2401 tattgacctt gaggggcagg taagtagtcc cgccctggct catcctgcgg ccgacctgcc
     2461 gtttggcgta ggttattggg atcctgcgct ggccctcgtt gacgaacacg atggcggcga
     2521 cgatcatgac ggccagaagc ccgaggataa ccaccgtgag cacgtcaccg ttctccctga
     2581 gcgccgtgat ggccaccggt gcctgcgaga gaatggaggc cgttatgatg agcgagatac
     2641 cgttgcccag gccccgctgg cttatgagct cgccgagcca catggtgagc atcacgccgg
     2701 ccgtgagggt aacgatggcg acgagtatct cgatggggct agtacctgcg agtgccccac
     2761 cgccctgcgc cgagcggaag aacagcacca tcgcgacgga ctggatgagc gccagggcga
     2821 gcgtgaagta gcgggtgtac tgcgtgattt tctgctgccc cgtctcaccc tctttggcga
     2881 gttcctgaag gcgtgggatg gcgaccgtca tgagctgcat cacgatcgct gctgttatgt
     2941 agggcatgat ccccagcgag aacacagaga agttgttgaa ggctccgccc gtgaacgtcc
     3001 cgaacaggcc cgttatgggg ttgctgctct gaagaccgcc gccgaggacg ctcgggtcga
     3061 ccccgggcag gggtatgtag gcccccagac ggtacgccgc cagcatggcg aacgtgaaca
     3121 ggagcttcat tcgcagctcc gggattttcc aggcgttggc tagggatccc aacatctcta
     3181 cttctccagt acctcgacgc ttccgccgcc agcttctatc ttctccctgg ccgctttgga
     3241 gaacgcgtgt gcctttaccg taacggcgcg ccctgcgtcc ccttgaccaa ggatcttgat
     3301 gagctcagcc ttcttcctga cgagcccggc ggcccgcaac tcgtcaggac ctatcgtatc
     3361 accgggaagg ccctggagtt cgccgaggtt caccggcgcg tagacggtag gacgggccac
     3421 cgtgtggcgg cccctggcct cgcccttccg gcgcggcaac cgccgctgca gcggcatctg
     3481 tccaccctcg taggccgcaa acgcggagac gccggagcgg gcccccgctc ccttgtgacc
     3541 acgtcccgaa gtcttaccgc gtcccgagcc cgtgccacgc ccgacgcgct tgggcgcttt
     3601 cttcgagcct ggagcaccct taatttcgtt gagcctcacc gctaatccac ctcctcggcg
     3661 tggacgaggt gctgcacctt gtggaccata ccccggatct ggggcgtgtc ctcgtgcacc
     3721 ctggaatccc tgatgcgctt gagaccaagg gcccgcaccg tccgcttgtg ccgctgcttc
     3781 gagcctacga cgctccttac ctgcgtgacc ttgagggggc tcatagtgtg atcttcaccc
     3841 ctcgcgtctg ctcgatgtcg gccttgctgc gcagcctctg gagcccttcg acggtggcct
     3901 gcacgaggtt caccgaggtc gtcgagccga gcgccttggt gagcacgtcc tttatgccgg
     3961 cgagctcgag gacggccctt actccgccgc cggcgatcac accggtaccc tccgaggccg
     4021 gcttgaggag taccgaagag ctctcgaact tgcctgtgac ctcgtgcggg atggtggtat
     4081 tgcgcatcgg tacgtcgaac atgttgcgct tggcccgctc ctggcccttc tggatggcgg
     4141 cggggacggt gttggccttc ccgagacccg tgccgaccct gcccttgccg tcgccaacga
     4201 ccaccagggc cgagaagttg aagcgccggc cgcccttgac caccttggcg acgcgccgga
     4261 tctcgacgac cctgtcctgg agatccgagg ccccgtcacg ttgccggccg ccgcgtcctc
     4321 ctccctgtct gttatcgctt cttggtcgtc ccaagatagt ccctcctaaa gagttagtcc
     4381 gccctggcgc gcgccctcgg cgagcgccgc gatgcgtccg tggtacttgt ttgggcccct
     4441 gtcgaagacg accgcctcta tcccggcctg ggaggcctct ttcgcgatca aggctccgac
     4501 cttgcgggct gcgtctttgc ggttctcgga ctcccccacc tggcgggagt cggccgccgc
     4561 gagtgtcttt cccgcgtcgt cgtcgatgag ctgggcgtag atgtgcacgt tggagcggta
     4621 caccgagagg cgcggcctgg aggccgtacc ggccaccttg cgcctgacac gcttgtgccg
     4681 cttctccctg tgcatccgct tttcgagaac agccatgaat cctcctacta ctatccggcc
     4741 ttgccgacct tgcgccggat ctgctcgtcg ctgtaacgga tgcccttgcc cttgtacggc
     4801 tcgggcggac ggatcatccg gatctccgcc gccgtctggc ctactctctg cttgtctatc
     4861 ccgcgcacga tgatcgtcgt ggcgttcgga acctcgaact ctatcccctc ccgaggtgag
     4921 atggagacag gatgggagta gccgacctgc agattgatgt cgcttccctg cagggctgct
     4981 ctgtagccga cgccggcgat gttcagggtc ttcgtgaacc cttccgtcac gccggttacg
     5041 gcgttgtaga tcagggatcg cgtaagcccg tgcatcgccc ggtgctcggg cgtgtccgcg
     5101 gcccgctcga ctattacgtt accgtcttcc tgtcttacat cgatcccgcg ggccacgggc
     5161 accgtgagct cgcccttggg gccgttcacc ttaacgctac ggcccgagag ctccacgctg
     5221 accgcgccgg ggatctcgac aggagctttc ccgattctcg acactgcaac ctccctagta
     5281 gacgaagcag aggacttcgc cgccgacgcc cctgcgcctg gcctggtggt ctgtcaggat
     5341 cccctgggag gtcgagagta tcgccacccc gagcccgtcg agcacgcggg ggatgttggt
     5401 ctgcttgcgg tagacccggc ggccggggcg gctcatccgt ctcaggccgg cgatgccgcg
     5461 gctaccgtcg gggccgtact tcaaggttat aacaagcctc ctctcgaccc cgctcccgcg
     5521 ctcggagacg tcctgaacgt agccttcctc cagaaggatt cgggagatct ccgccttcat
     5581 cttggagtgc ggtatctcga ccgtctcctg cttggccatg tgcgcgttgc ggatgcgcgt
     5641 caggaaatcc gctataggat cgttaaccga cataatcttc accaactcgc tttcgttacg
     5701 ccggggatct tcccctcgtg cgccagttct ctgaggcaga tccggcaaag cccgaacttc
     5761 ctgtagtaac cgtgcgcccg gccgcacctc tggcaacggt tgtactccct aaccgcgtac
     5821 ttctgctttt tctgctgctt taccagcaag gcctttctcg tcatatccta cctctcaact
     5881 ctgcctgaac ggcatcccga ggagccgcaa aagctcccgc gcctcttcgt cgctatccgt
     5941 cgtcgtcacg atcgccacgt cgagacctcg ggtcgcgtct atggagtcgt aggagatctc
     6001 ggggaagatc acctgctcct tgaggcctat cgcatagttt ccgcgcccgt cgaaagaagc
     6061 ggggctaacg cccctgaagt ccctgacgcg cggtagggct atggagataa agcggtccag
     6121 gaactcccac atgcgctcgc cgcgcagcgt gaccctggcc cccactggca tgccctccct
     6181 gatcttgaac ccggcgatgc tcttgcgcgc ccgcctgagc tgggccttct ggcccgtgat
     6241 cctggccagg tcctccatgg cgccgtcgag ggcgcggctg tttaccaccg cctccccgac
     6301 acccatgttc accacgatct tctcgagacc gggcatccgc atggagttct cgatgccgaa
     6361 cctctcctcg agatcgggga cgacctcatc gtggtacctt tccttcaacc tagccatcga
     6421 tatccttccc tgacttcttt ccgacccgga gcttctcacc ggagtctgta aagcggtaac
     6481 ccaccctcgt cggctcgccc gagttcggat cgatgagcat cacgttggag acgtctatgg
     6541 gagcctcgaa gtggtagagc ccctgctggt tctgctggct gggcctcgcg tggcgcgtgc
     6601 ggacgttgac gccaccgacg acgaccctgt tctctttggg gatgacccgt tcgatctcgc
     6661 cgcgtttgcc cttctctttg cccgagatca caacgacggt atccccgcgc tttagcttga
     6721 gacccataac tacaacacct ccggggcgag cgagatgatc ctggtgtaac ccttctccct
     6781 gagttcgcgc gcgacgggcc cgaagatacg cgtcccccgg ggtgcgccat cggggttgat
     6841 gatcac
//
LOCUS       DASTKM010000246         9060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_788731, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000246 DASTKM010000000
VERSION     DASTKM010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9060
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788731"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VFQ10_10095"
     CDS             <1..989
                     /locus_tag="VFQ10_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9965736.1"
                     /translation="LARYGATIEHHYGTPQDIEWALSGGDFFIVQSRPITALPEPMAD
                     PPTDWSVPVPKGTYWRASIVEQMPDPLSPLFADLAYGSVPRSLDKLIEELMGSGVFRE
                     GDIAFPTVNGYAYYYSLGAFWRLLVKTPGAFRLLMGSGERSGQRRWREYARPRYVRIV
                     EGWEAKPPENLSAKVLLAGARELLDAGTEYYTSVQTIIPLAYLSEALFTMFYNRLVRR
                     EGDPPAQTFLLGFDSMPILAEKSLYDLASWSREHPGLARALAGTPSEQILDLLGAEGP
                     PSGVDGEVWREWRSRFQAHLDRYGHAVYNPASTQYRPTTRAALRHAHVLPPG"
     gene            1054..1941
                     /locus_tag="VFQ10_10100"
     CDS             1054..1941
                     /locus_tag="VFQ10_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HET9965737.1"
                     /translation="MLERLDAPRRNLFSSLLKWAQDVAPVREDALADVGLAWPVMRRM
                     LLELGRHLTEAGAVEKPADVFWLRRDELEHTAASLDAGETHLASLADTVEQRRMLWRG
                     QRRVTPPQLLPENAWLEAFEGFMPAASEEQTGDTIKGVAASGGQVTAPARVLSGPADF
                     GQMQPGEVLVAGITTPAWTSLFAIASAVVTDVGGPLSHSSIVAREYDIPAVLGTGVAT
                     RRIQSGQTVQVDGDAGTVTLLDVPGAADTDQALPEPVSRAPREDVSTRKVALFALATG
                     AVVAAVTLWRRRRRVPKRQ"
     gene            2115..3104
                     /locus_tag="VFQ10_10105"
     CDS             2115..3104
                     /locus_tag="VFQ10_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965738.1"
                     /translation="MTLRLPDKWVWDFWLVRDGRDYHAFYLQAPRSLGDPELRHLNAS
                     VGHAASKDLRHWRVLPDALSAGPAGAWDDRAVWTGCVVRERGRWHMFYTGVSVEGDAV
                     VQRAGAATSEDLIRWRKHPKNPLIEADPRYYEQRDEGAVGYERMWRDPWVFKHRQTGD
                     YHALITARTIEGPSDGRGVIGHARSSDLVRWETLPPLTGPGHFYALEVPQLVELGERY
                     YLLFSTWAGAHSAARRKETRLEPVGGTHYLVADAPLGPFRFSTHDFLIGDPVGSLYSG
                     KLVEGPDGRPYFLAWRNIAPDGTFVGELADPFQVAVDGEGNLSVGRAQAHHLR"
     gene            3273..4049
                     /locus_tag="VFQ10_10110"
     CDS             3273..4049
                     /locus_tag="VFQ10_10110"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9965739.1"
                     /translation="MTSELEAALSAARRAGEVLRAAFGTEHSITYKGEVDLLTEIDEQ
                     AERLIREELLGTFPTYGMLAEEGGELTGEEDVRWIVDPLDGTTNYAHQLPIFCVSIAL
                     ERSGEVILGVVHDPMGEETFVAERGRGATLNGEPIKVSDTDELIRALIATGFPYDRAE
                     MPEALELFGRFAATTQGMRRLGSAALDLCYVASGRIDGYYERGIWPWDLAAGSVIVEE
                     AGGNLTNYRGDVLDLDGREIVASNGRLHPSIMRLMEEDDG"
     gene            4190..4672
                     /locus_tag="VFQ10_10115"
     CDS             4190..4672
                     /locus_tag="VFQ10_10115"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HET9965740.1"
                     /translation="MNESVLPKRSGSRPRTTPTNPHTQLEQNPEREVVDELATRVFAL
                     PGVEERPSAISVPGARALWLREELPAGPQEAFMIGREFAHIHPMPDGSLHAALPPEVA
                     QEAIKMGWAEQHPVARMGYLPQNVVMIYAPRDAQEIEIVARLVDEAYRYASGAHPSLP
                     "
     gene            5038..5418
                     /locus_tag="VFQ10_10120"
     CDS             5038..5418
                     /locus_tag="VFQ10_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965741.1"
                     /translation="MSEDSRQQFEVGESPLLKRILFGVFAGIFVTAIEFGGLFGISSS
                     SIREGILGILAAGCAFGVIIGVVPPWVGPEERFKWFCMFAIAGGAAGTTAWMIVQPDT
                     SLVVAVGANALLGGLYYLAGLVFG"
     gene            5493..6155
                     /locus_tag="VFQ10_10125"
     CDS             5493..6155
                     /locus_tag="VFQ10_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965742.1"
                     /translation="MNRSLLVAMIVLTVLYGCAQASNAVERVEKQHGLDKQVREQQTT
                     ELAPEPPTSEPIASEKPNLPEYNIMMDQDCSENFPQKCINAATDATSEEAFTLLTEHF
                     RDENPGYRAILVTFYENHQMPETTGSGWWFADEEVARTLLSQQYSDPQEANIDEQVRE
                     VMDNGGFHITSLQDEAQDMIEMACKDWDSKVMGTQPEFCHPHPASSPAFCHSASSPAF
                     CQ"
     gene            complement(6245..6688)
                     /locus_tag="VFQ10_10130"
     CDS             complement(6245..6688)
                     /locus_tag="VFQ10_10130"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9965743.1"
                     /translation="MVLRWIEAFNERDLEAEADVLAPDYVAPGYVAHVPAATGPLDLE
                     VLDAWRQFTTPFVEAFPDLRLTVEDIFSEGDMVATRVAFRGTHRGEFQGIPPTDKEVA
                     FSSMVSNHVVDGKVEENWVEIDLLRLLGQLGAIPELEHSEEANPT"
     gene            7135..7500
                     /locus_tag="VFQ10_10135"
     CDS             7135..7500
                     /locus_tag="VFQ10_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965744.1"
                     /translation="MQLPGQGEAAPARLLEQRSTKDHHKGEGPEVGERGRKGRDIERR
                     QGDRSEESAERHDRREAQEDEQVLPQAESPSHDPTQQATYARPPFGRARHDEGGHGRP
                     QQAGGGIGRPTCKVWPGGI"
     gene            7777..>9060
                     /locus_tag="VFQ10_10140"
     CDS             7777..>9060
                     /locus_tag="VFQ10_10140"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HET9965745.1"
                     /translation="MHWQVTPYSYLLLVAAAMSAALVFFAWRRRGTPGAETFALLMTG
                     VCIWAAGYALELSGADLPTKIFWAKAEYIGIATVPVVWLAFALRYTGREGQLTPRNLA
                     LLSALPLVTLLLAWTNEGHGLVWSSTGLNEDGPFPALEVDHGAWFWVHLSYSYLLLLI
                     GTILLISMLTRSPNLYRKQNLALLLAVSIPWVGNGVYVLGLSPVPNLDLTPFAFLLSG
                     MAIALSLFGFRLLDVVPVARANVLEGMADGVIVLDLQERVIDMNPAAERFLGCSARKS
                     IGRGFTGLVPNWSGLFEHYHGQAGEIHEEVSLGKETERRAYELTLSPVVDRKGHSRGR
                     LILLHDITERKRVEEEVRQLNATLEKRVADRTAQLADRERQLEDLVGKLMTAHEEERR
                     WLAYEMHDSLIQMVIAAQRYIQTFADTHPPGSKVRH"
BASE COUNT         1783 a         2713 c         2913 g         1651 t
ORIGIN      
        1 cgctggcccg ctacggggca accatcgagc atcattacgg aacaccgcag gacatcgagt
       61 gggcgctctc gggcggcgat ttcttcatcg tgcagtcccg tccgataacc gccctgcccg
      121 aaccgatggc cgatccgccg actgactgga gcgtgcctgt accgaagggc acgtattggc
      181 gcgccagcat cgtcgagcag atgccagacc cgctctcgcc gctgttcgcc gatcttgcct
      241 acggatcggt cccccgctcc ctcgacaagc tcatcgagga gctcatgggc tccggcgtct
      301 tccgagaggg ggacatcgca ttccccacgg tcaatggcta cgcttactac tactcacttg
      361 gcgcgttctg gcgtctcctc gtgaaaactc ccggagcctt ccggctcttg atgggcagcg
      421 gggaacggag cggccagagg cgctggcgcg agtacgctcg cccgcgctat gtgcggatcg
      481 tcgaagggtg ggaggcaaag ccgcccgaga acctgtcagc caaagtgctc ctcgctggcg
      541 ctcgggaact cctggatgca ggaaccgagt actatacctc ggtgcagacc atcatcccgc
      601 ttgcctacct cagcgaggcg ctgttcacca tgttctacaa ccggctcgtc aggcgtgaag
      661 gagatcctcc ggcacagacc ttcctgcttg gcttcgacag catgcccatc ctggccgaga
      721 agtcgctcta cgacctcgcc tcatggtccc gcgagcaccc cggcctcgcc agggcgctgg
      781 ccggcacgcc gtcggaacag atcctcgatc tgctcggtgc tgaaggacca ccctccggcg
      841 tggacggaga agtgtggcgc gagtggcgct cccgcttcca ggctcacctc gaccgctacg
      901 ggcacgctgt ctacaaccct gcgtcaaccc agtaccggcc gacgacccgc gccgctcttc
      961 gacacgctca cgttctacct ccggggtgaa ggacagaacc cccacaagcg ccaggggagg
     1021 acagccgcgc ggcgcgagga ggcgacgcaa gcagtgctgg agaggttgga cgccccgcgg
     1081 cgaaacctct ttagcagctt gctcaaatgg gcgcaagacg tagcgcccgt gcgggaggac
     1141 gcccttgccg acgtgggctt agcctggccc gttatgcggc gtatgctcct cgagctgggc
     1201 cgtcacctga cggaggccgg cgccgtggag aaacccgcag acgtgttctg gctgcgccgg
     1261 gacgagctag agcacacggc cgcgtcattg gacgcgggcg agacacacct tgcaagcctg
     1321 gctgacactg tggagcaacg caggatgctc tggcggggac agagaagggt tacgcccccg
     1381 cagctactcc ccgaaaacgc ctggctcgag gccttcgaag ggtttatgcc ggcggcatcc
     1441 gaggagcaga cgggcgacac catcaagggt gttgcggcta gtgggggcca ggtcacagca
     1501 ccggcccgcg tcctctctgg cccggcggac ttcggccaga tgcaaccggg agaggtgctg
     1561 gtggcaggca tcactacccc ggcatggacg tcgctcttcg ccatagcctc cgcggtggtc
     1621 accgacgtcg gcgggccttt gagccacagc tccattgtgg ctcgcgagta cgacattccc
     1681 gccgtgctgg ggaccggcgt cgcgaccagg cgcatccagt caggccagac tgtccaggtt
     1741 gatggagatg cgggcacggt aacgcttctg gatgtacctg gcgcggcgga caccgaccag
     1801 gcgctgccgg aacccgtgtc cagggcaccc agagaggacg tgagcacaag aaaagttgca
     1861 ctctttgcac tggccaccgg agcagtggta gcggccgtca ccttgtggag aagacggagg
     1921 cgcgtgccta aacggcagtg atcgacggac gtgacgcact caaggtagtc ccggacgagg
     1981 tgttgggctg ccgcactgcc acggaggtcc ccctgcgtaa ggggcacagg aggtgggctt
     2041 cgtaaactcg agccagttcg gaggccttcg gcttgtgatg attacacata actagaaggg
     2101 agaagtggtc agacatgacc ctgcggcttc ccgataagtg ggtgtgggac ttctggctgg
     2161 tccgagatgg cagggactac cacgccttct accttcaggc gccccgctct ttgggcgacc
     2221 cggagctgcg gcacctgaac gcctccgtcg gccacgccgc atcgaaggac ctgaggcact
     2281 ggcgtgtcct gccggacgcc ttgagcgccg ggccggcggg ggcctgggac gatcgcgccg
     2341 tctggaccgg atgcgtggtc cgggaaaggg gcaggtggca catgttctac acgggggtga
     2401 gcgtcgaagg cgacgccgtc gtccagcgcg cgggagcggc cacgtcggag gacctgatcc
     2461 gctggcgcaa gcacccaaag aacccgctca ttgaggccga cccgcgatac tacgagcagc
     2521 gggacgaggg cgccgtgggg tacgaacgga tgtggcgcga cccgtgggtc ttcaagcacc
     2581 gccagacggg agactaccac gcgctcatca ccgcccgcac catcgagggg ccatccgacg
     2641 gccgcggcgt gataggtcat gcccgctcga gcgacttggt ccgctgggag accttaccgc
     2701 cgctgacggg acccggccac ttctacgccc tggaggtgcc gcagctcgtg gagttggggg
     2761 aacgctacta cctgctgttc tccacttggg caggggcaca ctcggccgcg cgccgaaagg
     2821 aaacgcgtct cgagccggtg ggcggcacgc actacctcgt cgccgacgcc cctctgggcc
     2881 cgttccgatt ctccactcac gatttcctga tcggcgaccc tgtgggctct ctctacagtg
     2941 gcaagctggt agaggggccg gacggacgcc cgtatttcct ggcgtggcga aacatcgcac
     3001 ccgacggcac gttcgtgggt gaacttgccg acccattcca ggttgcagtc gatggtgaag
     3061 gcaacctctc agtggggagg gcccaagcac atcatctgcg atagctggat gtttctagtc
     3121 ccgcgcgtca tctgctgcga gcgcttcagc gacattcaag gtcgctctga aggacttgca
     3181 ggtcgtcaag ggcatcaccc tgccgctcgc ccgtcccgcc cccggcacgt taacatgtat
     3241 cgcacttgca ctcacacaaa gggatcgaga acatgacgag cgaacttgag gccgcactct
     3301 ccgcagccag gagggcgggc gaggtgctgc gcgccgcctt cggcacagaa cactccatca
     3361 cctacaaggg tgaggtggac ctactcacgg agatcgacga gcaagcggag cggctgataa
     3421 gggaagagct gttgggaacc ttccccacct acgggatgct ggccgaggag ggaggagagc
     3481 tcaccggcga ggaggacgtc cgctggatcg tggatccctt ggatgggacc accaactatg
     3541 cgcaccagct acccatcttc tgcgtctcca tagccctaga gaggtcaggc gaggtgatcc
     3601 ttggtgtggt gcacgacccg atgggcgagg aaaccttcgt cgccgagcga ggccgaggcg
     3661 ccaccctgaa cggcgagcct atcaaagtgt cggacacaga cgagctgatc cgggcgctta
     3721 tcgcgaccgg cttcccctac gatcgcgctg agatgcccga ggccctggag ctctttggac
     3781 gcttcgcggc gaccacccaa ggcatgcggc gcttgggttc tgcggccctt gatttatgct
     3841 atgtggcttc ggggaggata gacggctact acgagcgggg catctggcca tgggatctgg
     3901 ctgcgggaag tgtgatagta gaggaggctg gagggaacct aacgaactac cgaggagacg
     3961 ttctagacct cgatggcaga gagatagtgg cgagcaacgg acgtctgcac ccgtccataa
     4021 tgaggctgat ggaggaggac gacggatgag gtggttagta ctggccaact gtgttcgctg
     4081 caactccttc cctgaggagc ggcggaggga atctggagcg tgtactccct ggttttcgtg
     4141 gagtgttcgt atgaccaacg actgattggc aaggaggtgt agataagtga tgaacgaaag
     4201 cgtcttgccg aagaggagcg ggtcccgtcc caggaccacg ccaaccaacc cgcacaccca
     4261 gctggagcag aacccagagc gcgaggtggt ggacgaacta gccacgagag tcttcgccct
     4321 tcctggtgtg gaggagcgcc ccagcgccat ctccgtgccc ggagctcgcg ctttgtggct
     4381 gcgggaggaa ctcccggccg gtccacagga agcatttatg atcggtcggg aattcgccca
     4441 catccacccc atgccagacg gtagcctgca tgccgcgctg ccaccggaag tcgcacagga
     4501 ggccataaag atgggctggg ccgagcaaca ccccgtggcc cgtatgggct acttaccgca
     4561 gaacgtggtg atgatatacg ccccgaggga tgcgcaggag atcgagatcg tcgcccggct
     4621 cgtggacgaa gcctaccgct acgccagcgg tgcacaccct tctcttccgt aggcccttct
     4681 cgtaggggaa tgttcggcgg taggcaggca ctctgtcatg atagtcctcg taaaacggtc
     4741 ggtacagcac acctcgagaa cggtggggag ggaggagctc tcggccttcg agccgtctcg
     4801 cttgcgcttt gtgtgatctc gcgctattaa tggcactacc tcgcgcatta cgaggtagtg
     4861 gaaagcgaga ggatgaggga ctctcagcga ccgacagctg ggcagtggag cgccccaaga
     4921 aggacgccgg gtggcgggat cgggactgta cgtatctggc tgctggacgg cttctggcag
     4981 cgagctacga aggcgttcga aggaacataa cgaggcgctg gtagcagagg gcaattgttg
     5041 agcgaagata gtcgccagca gtttgaagtg ggggaatcgc ccctcctaaa gcgtatcctg
     5101 ttcggagtgt tcgcgggtat cttcgtgacg gccatagagt ttggaggcct cttcgggatt
     5161 tcttcctcat cgatacggga gggcatactg ggcattcttg cggcaggctg tgctttcggg
     5221 gtgatcatag gggttgtgcc gccctgggtg ggtccggagg agcggttcaa gtggttctgt
     5281 atgttcgcta tagcaggagg tgcagcaggg acgaccgcat ggatgatcgt ccagccagat
     5341 acctccctag tcgtcgcggt tggtgccaat gccctactcg gcggtcttta ctacctggcc
     5401 ggtttagtat tcggctgagc ttcttagaac tccgtcaagg cgaagttcga tgtatcatcg
     5461 ctctgcgcag tagggtgaat agggaaggaa gcatgaaccg tagcctgctg gtggcgatga
     5521 ttgtcttgac ggtgctctac ggatgcgcac aggccagcaa cgccgtcgag cgtgtggaga
     5581 agcagcacgg cctcgataag caagtccgtg agcagcagac gacagagctt gcacccgaac
     5641 ctcctacgtc cgaacctata gcttccgaga aaccgaacct ccccgaatac aacatcatga
     5701 tggatcagga ctgctcggag aactttcccc agaagtgcat caacgccgcc accgacgcca
     5761 cttcggagga agccttcact ctcctgacag agcacttcag ggacgagaac cccggatacc
     5821 gcgctatcct cgtcaccttt tacgagaatc accagatgcc cgagacgacc ggctcgggct
     5881 ggtggttcgc tgatgaggaa gtagcacgta ccttgctgtc gcagcagtat tccgatcccc
     5941 aagaggctaa catcgacgag caggtgcgcg aggtgatgga caacgggggc ttccacatca
     6001 cgtccctaca ggacgaagcg caggacatga tcgagatggc gtgcaaggat tgggactcca
     6061 aagttatggg aacacagcca gaattctgcc atcctcatcc tgccagtagc ccagccttct
     6121 gccattctgc cagtagccca gccttctgtc agtagctgta cctttagagt gaactcccta
     6181 gaactccatc aacgcgaagt tcgcagaatt taccttctac gaggttggat gatttaccta
     6241 tatgctaggt ggggttggct tcttcggagt gctccagctc ggggatggcg cctagctgcc
     6301 ccaaaagtct gagcaggtca atctcgaccc agttctcttc caccttgccg tccactacgt
     6361 ggttggacac catgctcgag aaggccactt ccttgtcggt cggcgggatg ccctggaact
     6421 cgccgcggtg ggtgcctcgg aaggccacac gcgtggccac catgtccccc tcggagaaga
     6481 tgtcctccac cgtgagccga aggtcgggga acgcctccac gaagggggtg gtgaactgcc
     6541 tccaggcatc caagacctcc aagtccaggg ggccggtcgc agccgggacg tgagccacgt
     6601 agccgggagc cacgtagtcg ggagccaaga cgtccgcttc ggcctctagg tcgcgctcgt
     6661 taaacgcctc gatccaccgt agaacgatgg tcgtgtgaga gccccgtgaa caacttccga
     6721 gaactctgtc agtgcgaagt tccgagaagg aaagaagagc cgggcaagcc tttaggaggg
     6781 ggagaggcct gagcccggta gcccagccgc cctcgcgtca cggcgatgat aaccagcgcc
     6841 cacgcggcta tggtgaggtt caggtagctg ccgacgacgg cggggcgggg aaaacctcca
     6901 cgaggccgcg ctgtagaagg tgttccacgt ggcgtgggtc aggatggcaa taagcacctg
     6961 tccccgtctt gttgaagacc cactgtatga tgtaggcgag ggcgatgagg gccaaggaga
     7021 acgttacgaa gtccatgacc gtgcctgacg ggggtagcat ttgcgggacc gagaacctgg
     7081 gcaggtgcca cagcgcccac aggctcccta ggatgaggcc ccaatcaagg ggccatgcag
     7141 ctgccgggtc aaggcgaagc cgcgccagcc cggctcctcg aacagcggtc caccaaggac
     7201 caccacaaag gcgaaggccc cgaggtaggt gagcgcggac ggaagggccg agacatcgaa
     7261 cgacgccagg gcgaccggtc ggaggaaagt gccgagcgcc atgaccgcag ggaggcccaa
     7321 gaggacgaac aggtgctacc gcaagccgag tcgccatcgc acgatccgac gcagcaggcg
     7381 acctatgccc gcccaccctt cggtcgcgcc cgtcacgatg agggcggcca cggaaggccc
     7441 cagcaggctg gcggggggat tggtcgccct acgtgcaagg tatggccggg cggcatatag
     7501 acgcactcac gtgtcgccga catactgcgg ggtgctagag aggaccattg gagaagtgag
     7561 caaccatcat cagaggtttg cacacgtatt gctcagcgtc gtcttcatgc gcctcaatca
     7621 ggctcgcaat caaggtctag taggtttgac cgaagttacc ttggtgggac gggccagttc
     7681 agtacctttg gtgttcgggt tggccaatgc ggcgcacttt gccaacgcaa acttctccgc
     7741 ctgctcgcgc aactacgggt tgcgccaagg attctgatgc attggcaagt caccccgtat
     7801 tcatatctgc tgcttgtggc tgctgccatg tcggccgcgc tagttttttt cgcctggcgt
     7861 cgtaggggca cgcctggcgc ggaaacgttt gcgttgctga tgactggcgt gtgcatatgg
     7921 gctgcgggat acgctctcga gctgagcgga gctgacctgc cgacgaagat attctgggca
     7981 aaggccgagt acattggcat cgccacggtt ccggttgtat ggctggcgtt cgccctccgg
     8041 tacaccggtc gcgaagggca gttgacgccc cgtaacctag cgctgctatc cgctcttccg
     8101 ctcgtcacgc tcctgctcgc gtggaccaac gagggccatg ggctcgtgtg gagctctacg
     8161 ggtttgaacg aagacgggcc gtttccagct ctggaagtag atcacggcgc ttggttctgg
     8221 gtccacttgt cgtattctta tctgctgctg ctgattggca ctattttgct gatttcgatg
     8281 cttacccgct cgccgaacct gtaccgtaag caaaacctcg cgttgctgct ggctgtgtcg
     8341 attccctggg taggcaacgg agtatacgtc ctaggtctca gccctgtgcc caacctggac
     8401 ctgactccat tcgctttcct gctaagtggt atggccatcg cgttgagcct gttcggtttt
     8461 cgcctgttgg atgtggtccc ggtagcacgc gcaaacgttc tagagggcat ggccgatggc
     8521 gtgatagtgc ttgacctgca ggagcgcgtt atagacatga atccggccgc cgagcgcttc
     8581 ctgggctgct ctgcccggaa atccatagga cgggggttca ctgggctcgt ccccaactgg
     8641 agcggattgt tcgaacatta tcacggacaa gcaggagaaa tccacgagga ggtcagcctg
     8701 ggcaaggaga ctgaacggcg agcttacgag ctaacgctct cgccggtggt agatcgaaag
     8761 ggacacagca gaggccgctt gattctgtta cacgacatta ccgaacgcaa gcgggtcgaa
     8821 gaagaggttc ggcagctcaa cgcaaccctt gagaagcgag tcgccgaccg caccgcccag
     8881 cttgccgacc gcgagcgcca gcttgaggac ttggtcggca agctcatgac agcccacgaa
     8941 gaggaaaggc gttggctagc ctacgagatg cacgacagtc tgatccagat ggtgatcgct
     9001 gcccaacggt acatacagac tttcgcagat actcatcccc cgggctccaa ggtaagacat
//
LOCUS       DASTKM010000247         3422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_434876, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000247 DASTKM010000000
VERSION     DASTKM010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434876"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFQ10_10145"
     CDS             <1..228
                     /locus_tag="VFQ10_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HET9965746.1"
                     /translation="TNGYRAIRVSELHKMNLREDRFNANEIILEALKHKLRFEQVPVS
                     MMSRAAGETKKPAKLAYPLGVFRVIISTWLR"
     gene            341..1603
                     /locus_tag="VFQ10_10150"
     CDS             341..1603
                     /locus_tag="VFQ10_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965747.1"
                     /translation="MLNRLKTGQGAGAGSVYIAASFAVAGALTFAFQSLCTHVLGKQG
                     FAPLALLWSATFLTVQVLWIGATQTLGRYVAQREAQGKDWGPVVSSVRHWQIGLVVAF
                     VLCALLASPLLTEELFEDPWLTVAFVVAVVLYAPEYFRRGIFNGHRQSFRLGAQIVAE
                     SSGRVLIAAVLLVVGWGVFGPVIAIVLAPLIGVLAIRPAPVAPPEREGEPFSAWHAFR
                     FATPVLACMAFAQVLMNGGPIFVRVLDGSDAQVSVFSAALIFTRIPQYVLSPAIGALL
                     PRASRVLSTEGRGVFDRFVAKALGVVGLVGVLMVAGTWMFGEWGLRLFAGAEFDASLA
                     VLVALATMAAFYLLSDAFNQGLFALGQGRLAALGWFAGLLASALCLTVLGVGVVERVA
                     YSLALGTFVAAVAQGMFYVGTRPGAVGR"
     gene            complement(1615..2598)
                     /locus_tag="VFQ10_10155"
     CDS             complement(1615..2598)
                     /locus_tag="VFQ10_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9965748.1"
                     /translation="MNWLVTGGCGFIGTALIRSLMEEGGHAVRVVDNLSVGTREDLAA
                     AGDFVEVSSDRLGPMVSDEQVELVVGDITDEDPAPHAALGADVIVHFAANTGVMPSVE
                     DPRGDCMSNVVGTLNYLEAARHAGVGRFIFASSGGTIVGEAEPPIHEEMPPHPVAPYG
                     ASKLAGEGYCSAYFSTFGVETVALRFGNVYGPLSGHKNSVVARFIKRAVEGEVLEIYG
                     DGTQTRDFIFVDDLIRAVRLSATVEDVGGEVFQIATSAETTVQELTARLLPALAAEGI
                     ENIEVRKTAARRGEVRRNYADTSKAQRMLGWKAEVGLDEGLRRTVEWFLGR"
     gene            2929..>3422
                     /locus_tag="VFQ10_10160"
     CDS             2929..>3422
                     /locus_tag="VFQ10_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9965749.1"
                     /translation="MKERILTRRDFLKVAGAGAAGATMLGAAGCGSLANSFTQVPDEY
                     LPSGGSRMNVIVVIIDSLRRDHLGVYGNDWIKTPNLDAVARESLRFDRSYPESLPTIL
                     ARRAIHTGIRTFPFRDWQKWYDEDVSLWGWQPIPRGQVTLAEILLGEGFYNLMVTDTL
                     HQFRP"
BASE COUNT          563 a         1088 c         1092 g          679 t
ORIGIN      
        1 acgaacggct accgcgccat aagggtctcg gagctccaca agatgaacct gcgggaggac
       61 cggttcaacg ccaacgagat catcctcgaa gccctcaaac acaagctgcg cttcgagcaa
      121 gtccccgtct cgatgatgag ccgcgccgcc ggcgagacca agaagccagc gaagctggcc
      181 taccctctgg gggtcttcag ggtcataatc tcgacctggc tgagatagag cttgtcagca
      241 tttcagcttc gtgtggtgtg tggtgaaacg ctcgtgcgct tgagacaatg gcatcggaac
      301 tcctactcat cgagagtcca ctccgaccgc gagacctgaa atgctgaaca ggctgaaaac
      361 agggcaggga gcgggtgctg ggagcgtcta catcgcggca tccttcgccg tcgcgggtgc
      421 actgacgttc gcgttccaga gcctctgtac tcacgtcctc ggcaagcagg gcttcgcgcc
      481 gctcgccctc ttgtggtcag ccacgttcct caccgtgcag gtactctgga tcggcgccac
      541 ccagaccctg gggcgctacg tcgcccagag ggaggcccaa ggcaaagatt ggggtcccgt
      601 cgtctcctcg gtcagacatt ggcagatagg gctggtggtg gcgttcgtac tctgtgctct
      661 gctggcgagc ccgcttctaa ccgaagaact attcgaagac ccgtggctta ccgtggcgtt
      721 cgtcgtcgcc gtggtcctgt acgcgcctga atacttccgg cgcggcatct tcaacgggca
      781 caggcagtct tttcggctcg gcgcccagat cgtggccgag tcgtctggca gggtgcttat
      841 cgccgccgtg ctgctcgtcg tgggatgggg tgtcttcggg cctgtcatcg ctatcgtgct
      901 tgccccgctc ataggcgtac ttgccatcag gcctgctccg gtggcgccgc cggaacggga
      961 aggagagcct ttctccgcct ggcatgcctt ccggtttgcc acgccggtgc tggcttgtat
     1021 ggccttcgcg caggtactga tgaacggcgg tccgatcttc gtgagagtcc tggatggtag
     1081 cgacgcgcag gtgagcgtgt tcagtgcagc actgatcttc acgcgcatcc cccagtatgt
     1141 cttgagcccc gccataggcg cgcttctgcc ccgggcgagc cgcgtccttt ccaccgaagg
     1201 caggggggtc ttcgatcgct ttgtggcgaa agccctcggg gtcgtcggcc tcgttggtgt
     1261 gctaatggtt gcaggcacct ggatgttcgg cgagtggggg ctcaggcttt tcgccggcgc
     1321 ggagttcgac gcgagcctgg ccgtgcttgt tgccctggcg acgatggccg ccttctacct
     1381 cctctccgat gcgttcaacc aggggctctt cgcactgggg caaggccggc tcgccgcgct
     1441 agggtggttc gccggtttgc tagcttccgc actctgtctg acggtgcttg gcgtcggtgt
     1501 cgtcgagcgg gtcgcatact ccctggccct cggcaccttc gtcgccgccg tggcgcaggg
     1561 tatgttctac gtggggacgc gacctggagc cgttggccgc taggcgcaaa gaccctacct
     1621 acccaaaaac cattccacgg tccgcctgag gccctcgtcg aggccgactt cggccttcca
     1681 gccgagcatc cgttgcgcct tggaggtgtc ggcgtagttg cggcgcactt ccccgcggcg
     1741 ggctgctgtt ttgcggacct cgatgttctc tatgccttcg gctgcgaggg cgggaaggag
     1801 cctggccgtc agctcttgca ccgtcgtctc ggcgctggtg gcgatctgga agacctcgcc
     1861 gcccacgtct tcgacggtgg ccgagagccg cacggcccgg atcaggtcgt ccacgaagat
     1921 gaagtcgcgg gtctgggtgc cgtcgccgta tatctccagc acttctccct ccacagccct
     1981 cttgataaag cgcgccacga cgctgttctt gtgccctgag aggggcccgt agacgttgcc
     2041 gaaccgtagt gccacggtct ctacgccgaa ggtgctgaag tatgccgagc aatacccctc
     2101 gcccgccagt ttgctcgccc cgtagggcgc gacgggatgc gggggcatct cttcgtggat
     2161 gggcggctcg gcctcgccca cgatggtccc gccgctcgag gcgaagataa agcgtccgac
     2221 gccagcgtgc ctggccgcct cgaggtagtt gagcgtgccg acgacgttgc tcatgcagtc
     2281 gccccgaggg tcctcgaccg agggcattac ccctgtgttc gccgcgaagt gaacgatgac
     2341 atcggctccc agggctgcgt gcggcgcggg gtcctcgtcc gtgatgtcgc ctacgaccag
     2401 ctcgacctgc tcgtctgaga ccatcgggcc cagcctgtcg gacgatactt cgacgaagtc
     2461 cccagcggcg gccaggtcct ctcgcgtccc cacagagagg ttatcgacga ctctgacggc
     2521 gtggccgcct tcctccatca gagaccggat cagggccgta ccgataaaac cacagccgcc
     2581 cgtgaccagc cagttcacgt ggaccgctcc agggagagag gatcttccat gtttcttgtg
     2641 cctgggtgca tacccgtcct tgccgagggt cgttggccgc ccatacctta gaccaacctc
     2701 cactccagcg cgagtctctg ggcgaaaggg gatctgaagg ccgtgctctt ggagagaact
     2761 cctgcggaag agggcgctat tcgctacaat cttcggcggc tggttcgact tagtagggaa
     2821 ggccttgatg tacacgaaga cgctctttgt tctacgagag cacgccgaac gcggcactcg
     2881 ggagatgtcc gggtattcgg catgccattg cgtaccagga gccgaggggt gaaggagcgt
     2941 atcctcaccc gcagggattt tctcaaggtg gccggtgcag gggccgccgg ggccaccatg
     3001 ctcggcgcgg ccgggtgcgg cagcctggca aacagcttta cccaagtgcc cgacgagtac
     3061 cttccgagcg ggggatcgag aatgaacgta atagtggtga tcatagacag cctgagaaga
     3121 gaccacctcg gcgtctacgg caacgactgg ataaagaccc ccaacctcga cgccgtcgcc
     3181 agggagagcc tgcgcttcga ccgctcttac cccgagtccc tgccgaccat cctggcgcgg
     3241 cgcgcgatcc acacggggat aaggacgttc cctttcaggg actggcagaa gtggtatgac
     3301 gaggatgtca gcctctgggg ctggcagccc atccctagag gacaggttac cctcgccgag
     3361 atcctgctcg gggagggctt ctacaatctg atggtcaccg acacgctgca ccagttcagg
     3421 cc
//
LOCUS       DASTKM010000248         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_261594, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000248 DASTKM010000000
VERSION     DASTKM010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261594"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFQ10_10165"
     CDS             <1..193
                     /locus_tag="VFQ10_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET9965750.1"
                     /translation="EDGQVVGSVSGGCVEPAVYEEAMAAIEGGEPRLLSYGISDDEAF
                     GVGLTCGGTIHIFVERVDW"
     gene            193..1011
                     /locus_tag="VFQ10_10170"
     CDS             193..1011
                     /locus_tag="VFQ10_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HET9965751.1"
                     /translation="MADLLSRWHEALAEQSPVAVATVIQGPAGSVGGKILISPEDHTG
                     TAGNDDLDRAIVEGARAMLEGGKTGTVHYGPRGQRRMEDVAVFIQSFAPPPRMYVFGA
                     IDFASAVARVGKFMGYRVIVCDARAVFATRERFPSADEIVVAWPDEFLKTAEVDGRSV
                     ITVLTHDPKFDVPVLKRALDTPAGYIGAMGSRRTHDNRTARLKEEGVTDEQLARISSP
                     IGLDLGARTPEETAIAIAAEIVALRTGHSGGRLAERSGPIHEAAPDTRTRDGVK"
     gene            1092..1973
                     /locus_tag="VFQ10_10175"
     CDS             1092..1973
                     /locus_tag="VFQ10_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET9965752.1"
                     /translation="MQVPARFEYEVAGSVGEAVELLERYGDGARLIAGGHSLIPMMKL
                     RLAAPEVVVDISGLDSELRYIEDEGGALRIGALTRHREVLESPVVRERYALLADAERM
                     IADPLVRNMGTIGGDLANADPAEDLPAAFVALDSEVLVRGPNGERTVHIDDLYVGPYE
                     TSLDHGEVLTEVRVPRTPGGSAYSKVKRKTGDYAAAAIGVALSMGGEEIESIRIGLCG
                     VGSRTLRARGAEDALTGGRPGSEAYRRAGEIAAEECDPIEDAKGSVEYKRDLVRVLLG
                     RTVARAVERASGLEVYR"
     gene            1970..>2323
                     /locus_tag="VFQ10_10180"
     CDS             1970..>2323
                     /locus_tag="VFQ10_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9965753.1"
                     /translation="MRIKVNVNGAMLEAEVEPRLLLVHFVRETLGLTGTHWGCDTSNC
                     GACTVLLDGEPVKSCTVLAVRADSHEVTTIEGLWSGTDLTPVQEGFHVEHGLQCGFCT
                     PGMIMTATEFLERNPD"
BASE COUNT          428 a          694 c          833 g          368 t
ORIGIN      
        1 cgaggacggg caggtcgtcg gttccgtgag cggggggtgc gtggagcctg cggtctacga
       61 ggaggcgatg gcggcgattg agggcggcga acccaggctc ctctcctacg gcatctccga
      121 tgacgaggca ttcggggtcg ggctgacgtg cggtgggact atccacatct tcgtcgagcg
      181 cgtggactgg taatggcaga tctactctcc cgctggcacg aggccctcgc cgagcagtcg
      241 cccgtggccg tggcgaccgt catacaggga ccggcgggtt ccgttggggg taagatcctg
      301 atctcccctg aggatcacac cgggacggcg ggcaacgacg acctcgacag ggccatcgtc
      361 gaaggggcca gggcgatgct cgaagggggc aagacgggta ccgtccacta tggcccgcgc
      421 ggccagcgca ggatggagga cgttgccgtc ttcatccagt ctttcgcccc gccgccaagg
      481 atgtatgtct tcggcgccat agacttcgct tcggccgtcg cgagggtcgg caagttcatg
      541 ggttaccgtg tgatcgtctg cgatgcccgc gccgtcttcg ccacccgcga gcgcttcccc
      601 tcggcggacg agatcgtcgt cgcctggccc gatgagttcc tgaagaccgc cgaggtcgac
      661 ggccgcagcg tcatcaccgt cctgacccac gaccccaagt tcgacgtgcc cgttctgaag
      721 agagcgctcg atacgccagc aggctacata ggggcgatgg ggagccgcag gacgcatgac
      781 aacaggacgg cccgcctcaa ggaggagggc gttacagacg agcaactcgc gcgcatcagc
      841 agtcccatcg gccttgatct aggggcgcgc acacctgagg agacggccat agcgatcgca
      901 gccgagatcg tcgcgctgag gaccgggcat tctggcgggc gcctcgccga acgatccggc
      961 cccatccatg aggctgcgcc agacaccagg accagggacg gagtaaaata gtccgctaag
     1021 gggtatttgt tccggtcctc tggccggaaa aaaggggaac tccaacgtcg ttggagagag
     1081 aggagagagg cgtgcaggtt ccggcgcggt tcgagtacga ggtcgctggc agcgtggggg
     1141 aagcagtcga gcttctagag cggtacggcg acggggcgcg cctgatcgcc ggcggccaca
     1201 gcctcatccc tatgatgaag ttgcggctcg ccgcgcccga ggtcgtcgta gacatcagcg
     1261 ggctggatag cgagctgcgc tacatcgagg acgagggtgg ggctttgagg atcggggcgc
     1321 tcaccaggca ccgcgaggtg ctcgagtctc cggtcgtacg ggaacggtac gcgctactgg
     1381 ccgacgcgga gaggatgatc gccgaccccc tcgtcaggaa catggggacg atcgggggtg
     1441 acctcgccaa tgcggacccg gcagaggatc ttccggctgc cttcgtcgcc ctcgacagcg
     1501 aggtcctggt ccgaggcccc aacggtgagc gtaccgtgca catcgacgat ctgtacgtcg
     1561 gcccctacga gacctccctc gatcacggcg aggtcctgac ggaggtccgg gtgccgcgca
     1621 ctccgggagg gagcgcttac tccaaggtca agcggaagac cggggactac gccgctgctg
     1681 cgatcggcgt cgcccttagc atgggcggcg aagagataga gagcatccgt atcggactgt
     1741 gcggcgtcgg aagccgaact ctgcgggcga ggggtgcgga agacgcgctc acagggggac
     1801 gcccggggtc cgaggcgtac aggagggccg gggagatcgc cgctgaggag tgcgatccca
     1861 tagaggacgc aaagggctct gtggagtaca agcgcgacct ggtcagggta ctcctcggac
     1921 gtacggtggc cagggcggtc gagcgcgcgt ccgggctgga ggtatatagg tgaggataaa
     1981 ggtaaacgta aacggcgcaa tgctggaggc cgaggtcgag ccgcgcctgt tgctcgtcca
     2041 tttcgtaagg gagaccctgg ggcttacagg aacccactgg gggtgcgaca cctccaactg
     2101 cggggcctgc acggtgctcc tcgacgggga acccgtcaag agctgtacgg tgctcgctgt
     2161 cagggccgac tcccacgagg tgacgaccat cgaggggctg tggagcggga cagatctcac
     2221 ccccgttcag gagggttttc acgtcgagca cgggctgcaa tgcggcttct gtacaccggg
     2281 catgataatg accgccacag aattcctgga gcgcaatccc gac
//
LOCUS       DASTKM010000249         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_789739, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000249 DASTKM010000000
VERSION     DASTKM010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789739"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..397)
                     /locus_tag="VFQ10_10185"
     CDS             complement(158..397)
                     /locus_tag="VFQ10_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965754.1"
                     /translation="MELRDFQAFGEPGAIVYLADCVDLMKIIPSGSVDVVFADPPYRL
                     SRGGVTVKSGKLAPVDKGAWDRSMGVVLIEATDYF"
     gene            complement(449..1567)
                     /locus_tag="VFQ10_10190"
     CDS             complement(449..1567)
                     /locus_tag="VFQ10_10190"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET9965755.1"
                     /translation="MRARELGIRIGDLSPGALDAITDVEGVRVGHSTIVRGEGPLVVG
                     EGPVRTGVTVVCPREGLARDEPVFAGSHRFNGNGEMTGLEWIREAGVLATPVAITNTH
                     SVGIVRDALVKAEIKARTDEDEYWCLPVVAETYDGTLNDINGMHVTAEHVFEALDSAS
                     TGPVEEGSVGGGTGMICHEFKGGIGTASRRLPQEGGGWTVGALVQANYGSRSVLRVDG
                     APVGRVLGTEIIPSPFEDGAQPAGTGSKIVVLATDAPLLPTQCDRLAMRASIGLGRMG
                     GGLDEGSGDIFFAFATGNRGIPRAGLYEPPATVPLQMVTNAHMSPLFHAAVEATEEAI
                     LNALLAAGDMMGRDGITAYGLTPESLLGALEEVRAPGT"
     gene            complement(1574..2347)
                     /locus_tag="VFQ10_10195"
     CDS             complement(1574..2347)
                     /locus_tag="VFQ10_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9965756.1"
                     /translation="MIEYLTIQDAEVPSLGLGTYRLTRGACVRAVGRALSMGYRHVDT
                     AQMYGNEAEVGRGVEDAGVDREEIFLTTKVWPSDFAHDRVIRKTRESLKKLRTDYVDL
                     LLMHWPGDGVPLGETLGAMRDLQEEGSVLHVGVSNFSPSLVEEAAEHAQIFCNQVQYH
                     PYRSQDALLAQAQEMDYLLTAYTPLSRGGVQRNKTLREIGEAHNKTATQVALRWLLQQ
                     DKVCAIPKATGEEHLRENLDVFDFELSDEEMDRIFSLGR"
     gene            complement(2392..2865)
                     /locus_tag="VFQ10_10200"
     CDS             complement(2392..2865)
                     /locus_tag="VFQ10_10200"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET9965757.1"
                     /translation="MKNPPALTRLEIRYGDLDPYGHVNNAVYLEFFEKVRICYWRALA
                     DMAGIEKLEAGDVPGARYVIAETTVRYKAPIFFEDTLHGAARMRTVGNRSYAMEFELR
                     TGETFEDGAIVAEGSAAHVFFDPLENTVQPRPGWFLPTVAELEGRPEASFSPEDS"
     gene            2945..>3374
                     /locus_tag="VFQ10_10205"
     CDS             2945..>3374
                     /locus_tag="VFQ10_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9965758.1"
                     /translation="MGYDEYLEQGETLASEGLVEEALSRFEQALEAAPENPEVIEAVG
                     RALLNLGRLEEAEASFLDALELDPEWVAPRMGLAMVALSRDEPFKIVHHLERAIEADS
                     GYPDAYVELGRYYGFMGEPALAKATFERWTNRVPEDADMLI"
BASE COUNT          581 a         1111 c         1036 g          646 t
ORIGIN      
        1 aaactccttc agtggggtgt tcatccagtc aatcccgctc attcttttct ccttacgcta
       61 agaggtacca atacctcttc taagcataac cctagcccgg ctcgttctct aacgccctct
      121 tatacatctc agcagccctg ctaccatcgt gcctgtgtca gaagtaatct gttgcctcta
      181 tgagtaccac gcccatggac ctgtcccagg cacccttgtc cacaggggcg agtttgccgc
      241 tcttgaccgt aacgccgcct ctggagagcc ggtaaggcgg gtcggcgaag accacgtcca
      301 cgctgcccga gggtatgatc ttcatgagat ccacacagtc cgcgaggtac acgatggccc
      361 ccggctcgcc gaacgcctgg aagtccctta attccacacc accattttag gcagcggcgt
      421 ccgccgactt tagcgacggt atagcgtttc aggtgccagg agcacggacc tcctcgagcg
      481 cgccgagcag ggactctggc gtcagaccgt aggcggtgat accgtcgcgc cccatcatgt
      541 cgccggccgc caggagcgcg ttcagtatgg cctcctccgt ggcctccaca gccgcgtgga
      601 agagcggtga catgtgggcg ttagttacca tctgcagggg cacggtcgcc ggtggttcgt
      661 agagcccggc gcgcggtatg ccgcggttgc ccgtggcgaa ggcgaagaat atgtcgccgc
      721 tcccttcgtc gaggccaccc cccatacggc cgaggccgat gctcgcgcgc atcgcgagcc
      781 ggtcacactg ggtaggcagg agcggcgcgt cggtggcgag gaccacgatt ttagaacccg
      841 tccccgccgg ctgggccccg tcctcgaaag gagaagggat gatctcggtg ccaagtacac
      901 gtcctaccgg tgcgccgtcc acgcgcagca cggagcggct cccgtagttc gcctgcacga
      961 gagcaccgac cgtccagccg cccccctctt gcggaagccg gcgcgaggcc gtgccgatac
     1021 cccccttgaa ctcgtggcag atcatacccg tccccccgcc gacagagccc tcctcaacgg
     1081 gtccggtact agcggaatcg agcgcttcga atacgtgctc cgccgtgacg tgcatgccat
     1141 tgatatcgtt cagggtaccg tcgtaggtct cggccaccac gggcaggcac cagtattcat
     1201 cctcgtcggt gcgggccttg atctcggcct ttaccagagc gtcgcgtacg ataccgacgc
     1261 tgtgggtgtt ggtgatcgcg acgggcgtcg ccagcacgcc ggcctccctg atccactcca
     1321 gccccgtcat ctcgccgttg ccgttgaacc tgtgagagcc cgcgaacacg ggctcgtcgc
     1381 gcgcaagccc ctcgcgggga cagactactg taacgccggt tctcacaggc ccctctccga
     1441 cgacgagcgg tccttcccca cgaacgatgg tcgagtgccc tactctcacg ccctcgacgt
     1501 cggtgatcgc atcgagagcg cccggcgata gatcccctat tcgtattcca agctcgcggg
     1561 cgcgcacgcg acctcagcgg cccagggaga agatacgatc catctcctcg tcggagagct
     1621 cgaagtcgaa gacgtcgagg ttctccctga ggtgctcctc gcccgtcgcc ttggggatgg
     1681 cacagacttt gtcctgttgc agtagccagc gcagggccac ctgcgtggcc gtcttgttgt
     1741 gcgcctctcc tatctccctg agcgtcttat tccgttggac tccgccgcgg gagaggggcg
     1801 tgtaggcggt cagcaggtag tccatctcct gcgcttgtgc gaggagcgcg tcctggctcc
     1861 tgtagggatg gtactggacc tggttgcaga agatctgggc gtgctctgcc gcctcttcga
     1921 ccagcgaagg ggagaaattg cttaccccga cgtgcaatac gctaccctct tcttgcaggt
     1981 cccgcatggc accaagggtc tcgccgagag gcacgccatc gcctggccag tgcataagca
     2041 agaggtcgac gtagtctgtg cggagcttct tcaagctctc tctggtcttc cggatgacgc
     2101 ggtcgtgggc gaagtcgctc ggccagacct tggtagtcag gaagatctcc tctctgtcta
     2161 cccccgcatc ctcgacgcca cgcccgacct cagcctcgtt gccgtacatc tgggccgtat
     2221 ccacgtgcct gtagcccatg gaaagggcgc gcccgacggc ccgtacgcac gcccctcgcg
     2281 tgagcctgta agtgccaagt cccagcgaag ggacctcagc atcctgtatc gtcagatact
     2341 ctatcatggc ctcctctaga gccaggcagt gttgttgcac cgtaacatgt tctagctatc
     2401 ctcaggggag aatgacgctt cggggcgacc ttccagctcc gctacggtgg gtaagaacca
     2461 gccagggcgc ggttgcaccg tattctccag agggtcgaag aagacgtgtg ccgcggagcc
     2521 ttccgcgacg atggcgccgt cctcgaaggt ctcgccggtg cgcagttcga actccatcgc
     2581 gtaggagcgg ttgccgaccg tgcgcatccg cgcagcgccg tggagggtgt cctcgaagaa
     2641 gatcggagct ttgtaacgta cggtggtctc ggcgatcaca tacctggcgc cggggacgtc
     2701 gcccgcttcg agcttctcga tgccggccat atccgcaagt gcgcgccagt agcagatgcg
     2761 gaccttctcg aagaactcca ggtagacggc gttgttgacg tggccgtaag ggtcgagatc
     2821 cccgtagcgg atctcgagcc gcgtgagagc gggagggttc ttcaccgagg cgtccccttc
     2881 ctatcggttt tccgcagaag attgtacata tcctcttcgc gtcccatctg atttaatgag
     2941 ggcgatgggc tacgacgagt acctggagca gggcgagacc ctggcgagtg agggccttgt
     3001 agaggaggcg ctctcgcgct tcgagcaggc cctcgaggca gcccccgaga accccgaggt
     3061 catcgaggcc gttggacgcg ccctactgaa cctgggccgg ctcgaggagg ccgaggccag
     3121 tttcctcgac gccctggagc tggaccccga gtgggtcgcg ccgcggatgg ggctcgcgat
     3181 ggtggcgctc tcgcgcgacg agccgttcaa gatcgtgcac cacctcgagc gtgccataga
     3241 ggcggactcg ggctaccccg acgcgtacgt ggagctaggc cgctactacg gctttatggg
     3301 ggagcccgcg ctggcgaagg ccaccttcga gcgctggacg aaccgcgtgc ccgaagacgc
     3361 cgacatgctc ataa
//
LOCUS       DASTKM010000250         6826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1493919, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000250 DASTKM010000000
VERSION     DASTKM010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493919"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..755
                     /locus_tag="VFQ10_10210"
     CDS             405..755
                     /locus_tag="VFQ10_10210"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9965759.1"
                     /translation="MARAATTTDAFNAVAEPWRRQILDILASGERPVNDLVRLLGLSQ
                     PQVSKHLRVLREVEAVDVRNEGRQRLYRLNGHALKPIHDWVKKYERSWSERFDRMEGV
                     LEELKKEGDMGGDE"
     gene            745..1224
                     /locus_tag="VFQ10_10215"
     CDS             745..1224
                     /locus_tag="VFQ10_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9965760.1"
                     /translation="MTSSGTATVTLPTDEQILIRREFEAPKHLVYKAWTTPELVKRWW
                     SGNRGEMTIAEIDLRVGGAWRYVMVTDEGIQVAFHGEYREIVPNERIVSTEVYEGMPE
                     GEALNTVTFTKTEGRTTLTVLVQHLSKEARDAHIDSGMETGMQEGMDLLEQVAVSLR"
     gene            1314..1754
                     /locus_tag="VFQ10_10220"
     CDS             1314..1754
                     /locus_tag="VFQ10_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9965761.1"
                     /translation="MSDTGITSGTPAARATSRRHAVNIFALWVLQALLAIMFAMAGFA
                     KVGGDAAMVEMFATIGIGQWFRYVVGVLEIAGAVGVLVSRLSGLAALGLLCLMAGAIL
                     TNVLVLGTSPLLPIVLMLVSALVAWGRWPRTRTLFTKREAGRLQ"
     gene            1751..1951
                     /locus_tag="VFQ10_10225"
     CDS             1751..1951
                     /locus_tag="VFQ10_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965762.1"
                     /translation="MTRVIFDISMSLDGFVTASNVRPEEPMGDGGQRLHDWAFGEDER
                     NRERNASAVPVGGVAEFGLAKT"
     gene            1972..2103
                     /locus_tag="VFQ10_10230"
     CDS             1972..2103
                     /locus_tag="VFQ10_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965763.1"
                     /translation="MANDDTNNAQAHQQTPDPNPDLENLERLVGTWEMSGDVRGTVT"
     gene            2392..2856
                     /locus_tag="VFQ10_10235"
     CDS             2392..2856
                     /locus_tag="VFQ10_10235"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9965764.1"
                     /translation="MDRQQHLDLGTSARTIIDSNFYMTLGTADERGRPWVSPVYYAHE
                     GYANFYWVSSPEANHSRNLVGRPEVSMVIFDSRTPVGSGQGVYISAIAEELMGADLDR
                     GIAIFSGRSDAHGAGEWNVEDVRPPARHRLYRATASEHWVLGPQDRRMPVSV"
     gene            complement(3320..3811)
                     /locus_tag="VFQ10_10240"
     CDS             complement(3320..3811)
                     /locus_tag="VFQ10_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9965765.1"
                     /translation="MSPRETEERTVKVNYFPTKILLATDASKDAVKAAQIASDMSNVS
                     GSDLHVLHVGSTKDFHVAPGAEQSFSPRSPSLGEVRENAEKTLEEAVKQVEEAGGTVA
                     ETHLRMGDPDDEILRHCEEHGDIGLLVMGSRGLGPIKRRLMGSVSESVVRHASCPVLV
                     ARS"
     gene            complement(3877..4644)
                     /locus_tag="VFQ10_10245"
     CDS             complement(3877..4644)
                     /locus_tag="VFQ10_10245"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET9965766.1"
                     /translation="MDAADRGIAIRTAHRRNRVSQTKRSQVTKAEPEDPRWNWLYKIG
                     GAAALFGVAIIPIQLIVFIAWGQPDTAIGWFTLFQDNKLAGLLAFELLLIVSVAVGIA
                     TTLALYIALRRVDESFMVIALALGLVEAVAFMLARPTFEMLYLSEGYAAATTDAQRAM
                     FLAAGEAMLATFTGTAFHLSINLFSIYFLIVSGVMLRSNIFGRVPAYMGILAAIFNWA
                     LYIPTIGLFLSILSVFPFLTIWNILVARRLFQLGKAS"
     gene            complement(4704..5465)
                     /locus_tag="VFQ10_10250"
     CDS             complement(4704..5465)
                     /locus_tag="VFQ10_10250"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET9965767.1"
                     /translation="MDETSSEPTERIMSPEREGHPVYGQIRPSGRMVRKVLLALGIVG
                     PLFYVATDILVATQWEGYSYTDQTVSELFAIGAPTRPLAVSLMLTYGALAIAFGVGVW
                     MSAGEKRALRVVAVALIGKEVLGSVATLFAPIHLREALAAGEGSLTDTWHGILTFGGA
                     LCYLLAMGFGATAFGKRFRLYSIATMLILVVFGVLTGLDQPQLEANLPTPWMGLWERI
                     DIFATMLWIAVLAIALLRAKVERPQDYFDGRSDAG"
     gene            complement(5828..5980)
                     /locus_tag="VFQ10_10255"
     CDS             complement(5828..5980)
                     /locus_tag="VFQ10_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965768.1"
                     /translation="MLATLGPKIIAGTAYGAAICTCCGVAENPPGPSPTAKSMPPHEM
                     ANARAK"
     gene            complement(6036..6701)
                     /locus_tag="VFQ10_10260"
     CDS             complement(6036..6701)
                     /locus_tag="VFQ10_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965769.1"
                     /translation="MGERHASIIERIVHRVEEHTEEWRQRDAARAAEAEANRDQLWAA
                     AVEREKHLTKIIGEEGQRASSEEIMKELRLAFVVHSEEVAQTLETFARKKDRLIKVIP
                     STRGSYAKEMGIKGSWLVFETSVPRSPQHTHDEPCTKHTVGQPKQCGHGEPCPAQLLE
                     DARHKSYRNTHQKKDRLVAGGEYAGEELGGEKREREEYRHDRERGGKERYRVPASANP
                     PPE"
BASE COUNT         1334 a         2035 c         2084 g         1373 t
ORIGIN      
        1 acgagttcgt cctctgcgac acccaacgtt gtcaggaccg taggggtggc ggttgaccct
       61 tcttggctat ctcggcgatc aggttctact ctcgcagtcc ctcgcgcgtg agcaacgatc
      121 ggtaggctcg gtgcaggttg atgctcgcct cttcaggcag acctccaacc gaggcagcag
      181 catgctccaa agtgcaaacc aaaagcggcg agtcgtggct gctttggccc cggcgctgat
      241 gctccctacg ggaataccct tcccgtccac agccacatcc ttaagctcaa gaccgattca
      301 gcggtcttga gccggacggt agcttccgca tcctgacgcg cacgttcgcc atcgagccac
      361 ggtctggggc ttgatatatt cccatatgag aatataattt tcctatggca cgagcggcga
      421 ccacaacgga cgcgttcaac gcggtggctg agccctggcg tcgacagatc ctggacatcc
      481 tcgccagcgg ggagcgtccc gtgaatgacc tggtgcgctt gctcgggttg tctcagccgc
      541 aggtgtccaa gcacctgcga gtgctccgag aggtggaggc ggtcgacgtg cgtaacgagg
      601 gacgccagcg gctgtaccgg ctcaacggcc acgcgctcaa gcccatccat gactgggtga
      661 agaagtacga gcgttcgtgg tcggagcggt tcgaccggat ggaaggcgtg ttggaggaac
      721 tgaagaagga aggagatatg ggcggtgacg agtagcggga cggcgacggt gacgctaccc
      781 acagacgagc agatcctcat caggcgggag ttcgaggcgc ccaagcacct ggtgtacaag
      841 gcgtggacca cgcccgagct ggtcaagcgt tggtggagcg gaaaccgcgg cgagatgacg
      901 atcgcggaga tcgacctgcg ggtcggcggc gcgtggcgat acgtgatggt gacggacgaa
      961 ggcatccagg tcgccttcca cggcgagtac cgcgagatcg tcccgaacga gcggatcgtc
     1021 tcgaccgagg tctacgaggg catgccagag ggggaggcgc ttaacaccgt gacgttcacc
     1081 aaaacggagg ggcgcacgac cctcacggtc ctcgtgcagc acttgagcaa ggaggcccgc
     1141 gacgcgcaca tcgactccgg gatggagacc ggcatgcagg aggggatgga cctactggag
     1201 caggtcgcgg tctcgcttcg ctgagcacgc gtctgcacag tgaaccacga ccatcataaa
     1261 ggagagctag tggctccgga gttgggtgag atccaagatc tgaggaggaa gtcatgagcg
     1321 acaccggtat cacgagtgga acgccggccg cgcgggcgac gagcaggcgg catgccgtga
     1381 acatcttcgc gctgtgggtg ctgcaggccc tgctggcaat catgttcgcg atggccgggt
     1441 ttgccaaggt gggtggcgat gcggcgatgg tggagatgtt cgccaccatc ggcatcggcc
     1501 agtggttcag gtacgtggtc ggagtcctgg agatcgccgg ggccgtaggc gtactggtct
     1561 cgcggctgtc ggggctggcc gcactcgggc ttctttgcct gatggcgggt gcaatcctga
     1621 ccaacgtgct cgtcctcggt actagcccgc tgcttccgat cgttttgatg ctggtgagcg
     1681 ccctggtcgc ctggggccgc tggccgcgga ccaggaccct gttcacgaaa agagaggctg
     1741 ggaggctcca gtgaccaggg tgatcttcga tatcagcatg tccctggacg ggttcgtgac
     1801 cgcctctaac gtcaggcccg aagaacccat gggcgacggc ggccaacgac tccacgattg
     1861 ggccttcggc gaggacgagc gcaaccgcga gcgcaacgca tctgcggttc cggttggtgg
     1921 agtagccgag ttcgggctgg cgaagacctg acagaaagga gaagccatct catggcaaac
     1981 gacgacacca acaacgccca agctcaccag cagacgccgg atccaaaccc cgacctagag
     2041 aacctggaga ggttggtggg cacgtgggag atgtccggag acgtgcgggg cacggtcacc
     2101 tagagcgtgt tatgaacttg ggcaaaagtc gcatagaaat gcggatttca cggtcagctt
     2161 ggttccctcg cgatctctgt ttcctgcatt cctaagccgt ttctaatgga gttcataaca
     2221 cgctctaggg agacgccttg gcgcttgtgg aagtcctggc ccagacaggc ctcgaagatt
     2281 gaccaggctt tggagccttg cttgatctgg ccagtgtcct catcgcgatt ggcgtagtcg
     2341 ttgaactggt ggctcttggc gccgggttgg gtagcacagg aggagaaggg catggatcgc
     2401 caacagcacc tcgatcttgg aaccagcgct aggaccatca tcgactccaa cttctacatg
     2461 acgctgggca cagccgacga gcgcgggcga ccctgggtgt cgcctgtgta ctacgcccac
     2521 gaggggtacg cgaacttcta ctgggtttcg tccccggagg cgaaccactc gcgcaacctc
     2581 gtggggcgcc ccgaggtgag catggtgatc ttcgactcca ggactcccgt cggctccggg
     2641 cagggggtgt acatctccgc tattgcggag gagctcatgg gcgctgacct cgacagaggc
     2701 atcgccatct tctctggcag atccgacgcg cacggggctg gcgagtggaa tgtggaggac
     2761 gtgcgcccgc ccgctcgcca tcgcctatac cgggccaccg cctcggagca ctgggtgctc
     2821 ggcccacaag accggcggat gccggtgagc gtgtaggatg ggttcctgga gttgcctatt
     2881 cactcgagtg catcgaagaa gaacattttc cgaagtgagg cgacggagcg ctaccacccc
     2941 aaggtcgtcg tctcgacgac ctttcaagag cccctcgagt ggaacaactc gactcagata
     3001 aaggggaacg tcgcggaaga gatcgacaat cgctcagcgc ttgtcgtgtg aacgtgggcc
     3061 gctatcgttt ggagaaagct aggccagaac aaagctgcct ggatctggca tcattaggta
     3121 cgataagggc cggtttacag cgacacatga aggatttatc agtatctacg aacgtgctgc
     3181 aaagtactca tttttggctt atcttggagc ggagaatcgt cgccagacgc tggtggaccc
     3241 actcatgagg tacgtctcga agcggcgcag ggaggcccag gtcgagtaat gtgcgacgga
     3301 gggttcctgc ggtacagctc tagctgcgcg ccacaagaac cgggcagctt gcgtggcgga
     3361 cgacgctctc ggagacgctc cccatgagcc tgcgctttat gggacctagt ccccggctcc
     3421 ccatgacgag caggccgatg tcaccgtgct cctcgcagtg acggagtatc tcgtcgtccg
     3481 gatcgcccat cctcaggtgc gtctccgcga cggtgccccc ggcttcctct acttgcttca
     3541 cagcctcctc gagcgtcttc tcggcattct cccggacctc acccagggaa gggctgcggg
     3601 gggagaaact ctgctccgca ccaggggcga catggaagtc cttcgtgctt ccgacgtgga
     3661 ggacatgaag gtccgagccg ctgacgttcg acatgtcgct agctatctgg gcggccttga
     3721 ccgcgtcctt cgaggcgtcg gtcgctagca ggatcttcgt cgggaagtag ttcaccttta
     3781 ccgtcctttc ctccgtctcc ctcggactca ttttaccagt ggtgctacca gtcaagccat
     3841 ctctggaaaa gctggtcagc atttcagggt gctacctcaa gatgccttac caagctggaa
     3901 gagccttcgg gcaaccagga tgttccatat cgtcaagaag gggaagacgg aaaggatcga
     3961 gaggaagaga cctatcgtcg gtatatagag ggcccagttg aatatggccg caagaattcc
     4021 catgtaggcg ggtactctgc cgaagatatt gctgcgcagc atgacgcccg agactatcag
     4081 gaaatagatg ctgaagaggt tgatactcag gtggaaagca gtgcctgtga aggtagccaa
     4141 catcgcttcc cccgccgcca ggaacatggc cctttgggcg tccgttgtcg cggccgcgta
     4201 cccttcgctg aggtagagca tctcgaaggt cggacgcgca agcatgaagg ccacggcctc
     4261 cacgagtcca agggccagag cgatcaccat gaaagattcg tcgactcgtc tgagggcgat
     4321 gtagagggcg agggtcgtcg cgatcccaac agcaacgcta acaattagca ggagttcgaa
     4381 agccaggagt cccgcgagct tgttgtcctg gaacagagta aaccagccga ttgcggtgtc
     4441 gggttgcccc catgcgatga agacgatgag ctggatcggg ataatcgcca ctccgaacaa
     4501 ggcggccgcg ccccctatct tgtagagcca gttccagcgt ggatcttctg gttctgcctt
     4561 tgtaacctgg cttcgcttcg tttgcgacac tctatttctc ctgtgcgctg ttcttattgc
     4621 aattccacga tccgctgcgt ccattgcgcg gctcgctcga gttcgccttc tattagatgt
     4681 catgtcgggc gattggaccc gactcatcca gcatcacttc tcccatcgaa ataatcttgg
     4741 ggccgttcca ctttggcgcg taagagtgcg atggccagca ccgcgatcca cagcatggtg
     4801 gcgaagatgt cgatgcgctc ccaaagcccc atccacggcg tgggcaagtt tgcctcaagc
     4861 tggggctggt ccaaacctgt caaaacgcca aacactacga gtatcagcat tgtcgcgatc
     4921 gaatagaggc ggaaccgctt tccgaatgcc gtagccccga accccatggc gagcaggtag
     4981 cagagagcac ccccaaacgt gagaatcccg tgccaggtat cggtcagcga tccctcaccc
     5041 gctgccaggg cctcacgcag gtgtatcggg gcgaatagcg tcgccacgga gccaaggact
     5101 tcctttccga tcagcgcgac cgccacgacg cgcagggcgc gcttttcacc agccgacatc
     5161 cagactccca ccccgaacgc gatcgcaagc gcgccgtacg tgagcataag cgaaacagct
     5221 agcggtctcg tcggagcgcc gatggcgaac agctcgctga cggtttgatc agtgtagctg
     5281 tagccctccc attgcgtagc tacaaggatg tcagtggcga catagaacag cggccccacg
     5341 atgccgaggg cgagcaagac ctttcgtacc atccttccgc tcggcctgat ctgtccatag
     5401 acggggtgcc cttctcgctc cgggctcatt atgcgctcgg ttggttcgct gctggtctca
     5461 tccattagcg actccttccc gcaggctgga agcgggcaca taggcggccg cccctctatt
     5521 atgacgtcct tagactaact ctggagtaga aggctgtcat ggcatacatt gcccagttta
     5581 gtggtcaagt tgtgccatta gcttgcagaa cttcagagac gttctcttcc aggcacttcg
     5641 gtgaatactc ctcgtagccg cctagcatga gatctgatca aggtacacca gcagaatgct
     5701 caatatgtgt catggtagat caccttcctc gtagcacact gagactgcag ttatcctcga
     5761 ccacagaaag gaggcggtat ctcatgaaga ccatcaaggc tttcatcaag agacacccgc
     5821 tgctgagcta cttcgccctg gcgttcgcca tctcatgggg cggcatgctc ttcgcggtcg
     5881 ggctcggtcc tggcggattc tcggccaccc cacaacaggt acagatggcg gccccgtacg
     5941 ctgtcccggc gatgatcttg ggccccagcg tggcgagcat cctcctgacc ggcctcctct
     6001 atggaagagc tggccttcgc gagtttcgat cccggctact caggtggcgg gttggcgctc
     6061 gctggtacgc ggtagcgctc cttaccgccc cgctctcgat catggcggta ctcctcgcgc
     6121 tctcgcttct ctcccccgag ttcctccccc gcatattcac cgccagcgac aagcctatcc
     6181 tttttctgat gggtattgcg gtaggacttg tggcgggcat cttcgaggag ctgggctgga
     6241 cagggttcgc cgtgcccaca ctgcttaggc tgccctacgg tgtgcttagt acagggctca
     6301 tcgtgggtgt gctgtgggga gcgtggcacc gacgtttcga agacgagcca cgagcccttg
     6361 atgcccatct ccttggcgta gcttcccctg gtgctaggga tcaccttgat caaacgatcc
     6421 tttttgcggg cgaaggtctc gagtgtctgg gcgacctcct cggagtgcac cacgaaggcc
     6481 aaccggagct ccttcataat ctcttccgaa gaggctcgct gaccttcttc tcctatgatt
     6541 ttggtcaggt gcttctccct ctccaccgcc gcggcccaca gctggtcacg gttggcctca
     6601 gcctcggcag cacgggcggc atcccgctgg cgccactcct cggtgtgctc ctcgacccgg
     6661 tgcactatcc tctcgataat ggacgcgtga cgttcgccca ttccttacct cctcgctctc
     6721 ctgactgctc gcgcaccaag taacctggct acgtggctcc agagcacaac ctcccactca
     6781 gtcctaacgg tatagatagg cctcgagacc tctgttgggc tcgtgt
//
LOCUS       DASTKM010000251         4479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_262360, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000251 DASTKM010000000
VERSION     DASTKM010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262360"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..112
                     /locus_tag="VFQ10_10265"
     tRNA            23..112
                     /locus_tag="VFQ10_10265"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55..57,aa:Sec,seq:tca)
     gene            257..712
                     /gene="moaC"
                     /locus_tag="VFQ10_10270"
     CDS             257..712
                     /gene="moaC"
                     /locus_tag="VFQ10_10270"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET9965770.1"
                     /translation="MTFSHLDSEGAARMVDVGEKEVVRRIAVAGAVMRMSSETIGLLR
                     KAALPKGDVLNTARIAGVMAAKKTPELIPLCHGLNLTSVDIEFGVGQDRVSITATTRA
                     SDRTGVEMEALTAASVAALTIYDMCKAVDKGMVIGELRLLEKTKEPANP"
     gene            869..1393
                     /locus_tag="VFQ10_10275"
     CDS             869..1393
                     /locus_tag="VFQ10_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HET9965771.1"
                     /translation="MNLSGRRCVVVGGGAVATRKVGKLLRAGAEVVVVSPEVRPELAG
                     MDVEILHRPYESGDLEGAKLAFAATDSREVNALVAQEAKERGVPINVADRPAEGDFTV
                     PSTLRRGGLQVAVSTGGASPTLARRIRSELEEAFAPEWARVVKEFDTARRGGGATGLD
                     LEEEVSRCLSQLRG"
     gene            1369..2664
                     /gene="hemA"
                     /locus_tag="VFQ10_10280"
     CDS             1369..2664
                     /gene="hemA"
                     /locus_tag="VFQ10_10280"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET9965772.1"
                     /translation="MLVAVAGMSHRSAPIEVRERVAFPPCAGRSFLRRLKEEGVVSEA
                     VLLSTCNRTEVYAVVEDEGARARVLDLLAEDRRVERSSLEQDTYWLTDAEAVRHLYRV
                     ASSLDSMVVGEGQILGQVREAYRASTEVHCAGQILNRLFHTSLRVGKKVRSETGIGDS
                     SLSVPRVAVKLAEEVFGDLAGRRALVLGAGDMSELVVKHLKDRGVADLLIANRTPQRA
                     SLLAERVGGKAVAFDALAAELPEVDVVVSSTGSGEWVVQSETVAGALALRSEPLFFID
                     IAVPRDIDPVVQTLGSVYLYDIDDLQAVVERNAEGRQEAAEEGEAIISPTVPEFMGWL
                     STLHVVPLIQELRDEAEQIRRHELARALSRMELSPEEMASVERMSYSLVNKLLHGPIQ
                     EIKARAEAGSPLESSEVRRRLMALDGLDVELHRPRRESP"
     gene            2661..3584
                     /gene="hemC"
                     /locus_tag="VFQ10_10285"
     CDS             2661..3584
                     /gene="hemC"
                     /locus_tag="VFQ10_10285"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET9965773.1"
                     /translation="MTPEYTAILGTRGSRLALAQAEECAASLRAAGTAVEIRIIRTTS
                     EHHPDTPLAVIDQRDVFTRQLDEALLSGEIDLAVHSLKDVPTEPPEWVDLAAFTLRRD
                     PSDVLVSDGRHTLEDLPQGAVVATSSQRRRAQLLHQRPDLEVVGIRGNVDTRVRKMRE
                     GAADAVVLARAGFERLGLDAPHTVIPADKVLPAVGQGALAAATLHDHPLRGLVRRVLN
                     HGPTERSVLAERAMLRALEGGCRVPVGAISAVRGTVVRLRGVVVSPDGALAYRGEAEG
                     EEPEEVGERLARDLLEQGAAAVLEEIREVRR"
     gene            3586..>4479
                     /gene="cobA"
                     /locus_tag="VFQ10_10290"
     CDS             3586..>4479
                     /gene="cobA"
                     /locus_tag="VFQ10_10290"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HET9965774.1"
                     /translation="MIYLVGSGPGDPGLFTLKGVRCLLEADAVVYDRLAPRALLGYAR
                     PEAEKIYVGKKPGDPTMSQEEINDLLVEKGRAGMTVVRLKGGDPYIFGRGGEEALALK
                     RAELPFEVVPGVTSGVAAPAYAGIPVTHRNVSTSVAFVTGHEDPTKGSSDVDWERLAN
                     GADTLVLYMGVGRLEEISKELIAAGKSRETPVACVRWGTVPQQRTVTGTLEDIADRVA
                     EAGLRPPAVVVVGDVVALREEGLDWYERRPLFGRRVVVTRARAQAGELSADLERLGAE
                     VHEFPTIEISPPEDFGPLDAAI"
BASE COUNT          807 a         1361 c         1576 g          735 t
ORIGIN      
        1 ataaactcta cccgccgaac caggaatgcg tgggcctggt ggcccggccg gtcttcaaaa
       61 ccggtttgcg accgtaagtg cggccgtggc ggttcgattc ccccgcattc cgcgaaaaaa
      121 gcaagtcagc aagccgcttg cagcggcttg tcagcttgtc agccggggct cgcacaagcg
      181 aaagctgaaa tgctgacaag cgcccgatag ggcgcgtgct gaaatgctga ctagctgccg
      241 accgaaggga ggcaatatga ccttttctca ccttgacagc gagggcgcgg ccaggatggt
      301 cgacgtgggg gagaaggaag tggtgcggcg tatcgcggtc gccggggcag taatgcgcat
      361 gtcctcagag acgatagggc tgttgcgcaa agctgcgttg cccaagggcg atgtgttgaa
      421 cacggccagg atcgcaggtg tgatggcggc caagaaaacg ccagagctca taccgctgtg
      481 ccacgggttg aacctgacct ccgtggacat cgagttcggg gtcggccagg atcgcgtgtc
      541 gatcaccgcg acgacccgtg cgagcgaccg tacgggcgtc gagatggagg ccctcaccgc
      601 tgctagcgtg gccgcgttga ccatctacga tatgtgcaag gcagtcgata agggcatggt
      661 tataggggag ctcaggttgt tggagaagac caaagagcca gccaatccgt gaaggcttac
      721 acggcgcccc gcgacggtgc atgcaacact ttgtgagaca acgatacccg gttggtggtt
      781 atgctagact ttcaggctaa tccacaggcc attctctagg tcaggagtag agatcgacgg
      841 cggaaatacc gttctttacc ccatatttct gaacctctcc ggcaggcgtt gcgtggtagt
      901 cgggggtggg gcggtggcca ccaggaaggt cggtaagctg ttacgagcgg gcgccgaggt
      961 cgttgtggtc tcccctgagg tccgtccgga gcttgcgggc atggacgtgg agatcctaca
     1021 ccgtccctac gaatccggcg acctcgaagg cgcgaaactg gcgtttgccg cgacggactc
     1081 gcgagaggtc aacgcgttgg tcgcacagga agcgaaggaa cgcggggtcc ccatcaacgt
     1141 cgccgaccgt cctgctgagg gggacttcac cgtcccgtcg acgctgcgga ggggtgggct
     1201 ccaggtcgcg gtctccaccg gcggggcgtc gcccaccctg gcgcggcgga tcaggagcga
     1261 gttggaggag gccttcgcgc ctgagtgggc ccgtgtagtg aaggagttcg acacggcccg
     1321 tcgcggcggt ggggcaacag gcctggacct cgaagaggag gtgagccggt gcttgtcgca
     1381 gttgcgggga tgagccaccg ctcggcgccg atcgaagtgc gcgagcgcgt cgctttcccg
     1441 ccgtgcgctg gccggagttt cctcaggcgc ctcaaggagg agggcgtagt ctcagaagcc
     1501 gtgctgctct cgacctgcaa caggaccgaa gtctacgccg tcgtcgagga tgagggagcg
     1561 agggcgcggg tgctcgacct gctcgccgaa gacaggcgcg tcgagcgatc gtcgctcgag
     1621 caggacacgt actggctgac ggacgctgag gccgtgcggc acctgtacag ggttgcctct
     1681 tcgctggatt cgatggtcgt cggggagggt cagatcctcg gccaggtccg cgaggcgtac
     1741 agggcgtcca ccgaagtgca ttgcgcgggc cagatcctca atcggctctt ccacacatcg
     1801 ctcagggtcg gcaagaaggt ccgctccgag acggggatag gcgatagctc tctctccgtg
     1861 ccgcgcgtcg ccgtgaagct agccgaggag gtcttcgggg atctcgcagg aaggcgggcc
     1921 ctggtcctcg gcgcgggtga catgagcgag ctcgtggtca agcacctcaa agacaggggc
     1981 gtcgccgacc tcctaatcgc caaccgtaca ccccagcgtg ccagtctgct cgccgagcgt
     2041 gtggggggca aggccgtcgc gttcgacgcc ctcgcggccg agctacctga ggtggacgtc
     2101 gtcgttagct ccacaggctc cggcgagtgg gtcgttcaga gcgagacggt cgcaggggcg
     2161 cttgcgctgc ggagcgagcc gttgttcttc atagacatcg cggtgccaag ggacatagac
     2221 cccgtggtcc agaccctggg gagcgtctac ctctacgaca tagacgacct ccaggccgtc
     2281 gtcgagcgca acgcggaggg tcgccaggaa gcggccgagg agggagaggc catcatctct
     2341 cccacggtcc cggagttcat gggctggctc tcgacgctgc acgtcgtgcc gctcatacag
     2401 gagcttcgcg atgaggccga gcagattcgc cgtcacgagc tcgcccgcgc cctctcgagg
     2461 atggagctct cgcccgagga gatggcttcc gtcgagcgca tgagctactc tctcgtcaac
     2521 aagctgctcc acgggccgat ccaggagata aaggcccgcg ccgaggccgg ctccccactc
     2581 gagagctctg aggtaaggcg ccgcctgatg gccctggacg ggctcgacgt ggaactgcac
     2641 cgcccgcgcc gcgaatcccc gtgaccccgg aatacaccgc tatcctcggt acccgcggct
     2701 cgaggctcgc cctcgctcag gctgaggagt gcgcagccag cctgcgcgcc gccggtacag
     2761 cggtggagat ccgtatcatc aggacgacca gcgagcacca ccccgacacg cctctcgccg
     2821 ttatcgacca gcgcgatgtc ttcacccgcc agctcgacga ggccctcctt tcgggcgaga
     2881 tagacctcgc cgtacactct ctgaaagacg taccgacgga gccaccagag tgggtagatc
     2941 tcgcggcctt caccctccgc cgcgacccct ccgacgtgct cgtctcagac ggacgtcata
     3001 ccctggaaga tctgccgcag ggggctgtgg tggccacctc cagccaacga cggagggcgc
     3061 aactcctgca ccagaggccg gatctcgagg tcgtcggcat caggggcaac gtggatacgc
     3121 gcgtgcgcaa gatgcgcgag ggcgcggccg acgcggtcgt gctggcccgg gccggcttcg
     3181 aacgcctcgg cctcgacgcc ccgcacaccg tcattcccgc cgacaaagtg cttcccgccg
     3241 tcgggcaggg tgccctggcc gcggcgacac tgcacgatca tccccttcgc ggtctcgtta
     3301 gaagggtcct gaatcatggg ccgaccgaga ggtctgtgct cgccgagcgg gccatgctcc
     3361 gcgcgctcga aggtggctgc agggtacctg tcggggcgat cagcgccgtg agagggaccg
     3421 tggtgcggct aaggggcgtt gtagtctccc cggacggtgc gctggcgtat cgtggtgagg
     3481 ctgagggcga ggagcccgag gaggtgggag agaggctggc gcgggacctc ctggagcagg
     3541 gggcggccgc ggtgcttgag gagatcaggg aggtcaggag atagggtgat ctacctggtc
     3601 gggagcgggc ccggggaccc tggactcttt accctcaagg gcgtgcggtg tctgctggag
     3661 gccgacgccg tcgtctacga caggctcgcg ccaagagcgc tgctcgggta cgcgaggccg
     3721 gaagccgaga agatctacgt cggcaagaag cctggcgatc caacgatgtc ccaggaggag
     3781 ataaacgatc tcctcgtcga gaagggccgc gccgggatga cggtcgttcg cctgaagggg
     3841 ggcgaccctt acatcttcgg acgtggcggt gaagaggcgc tcgcgctgaa aagggccgaa
     3901 ttacctttcg aggtcgtccc tggtgtgacg agcggtgtcg ccgccccggc atacgccggg
     3961 atacccgtta cccacaggaa cgtctcgacc agcgtcgcgt tcgtcacggg gcacgaagac
     4021 ccgacgaaag gcagttcgga cgtggactgg gaaaggctgg ccaacggcgc cgatacgctc
     4081 gtcctgtaca tgggcgtagg caggttggag gagatctcga aagagctcat cgcggctggg
     4141 aagagcaggg agacaccggt cgcctgcgtc aggtggggca ccgtacccca acaacggacc
     4201 gtcacaggaa ctctggaaga catcgctgac agggtagccg aggcaggctt gaggccgcct
     4261 gcggtcgtcg tcgtcgggga tgtcgtcgcc ctgcgcgaag aagggctcga ctggtacgag
     4321 cggcgtccgc tcttcggacg tcgggtggtc gttacgcggg cgagggcgca ggctggtgag
     4381 ctatccgccg atctggagag gttaggagcc gaggtccacg agtttccgac catagagata
     4441 agtccccccg aggacttcgg accgctcgac gcggcgatc
//
LOCUS       DASTKM010000252         8010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_613190, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000252 DASTKM010000000
VERSION     DASTKM010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613190"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFQ10_10295"
     CDS             complement(<1..401)
                     /locus_tag="VFQ10_10295"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9965775.1"
                     /translation="MTVIVALLGLIFLIVIHELGHMLTAKALGVRVPEFGVGFGPALV
                     KKKFGNTVYSFRIILLGGFAKMAGMGDGAEGRDTYYAKALWRRALIILAGPGANLLAA
                     VAILACVYMFQGVVTDVKPVVSAVERGSMAQ"
     gene            complement(398..1537)
                     /gene="dxr"
                     /locus_tag="VFQ10_10300"
     CDS             complement(398..1537)
                     /gene="dxr"
                     /locus_tag="VFQ10_10300"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET9965776.1"
                     /translation="MRRRLTILGSTGSIGRQALDVVSRYPERFEIVGLSAGNDARALA
                     LQAAEFRPEYVALESSEAGPLEDLASEVFAGPGSAEQLSRAPSDVVLNGIVGFAGLAA
                     TVGALQEGNRVALANKESLVAGGEWVMDLAASNRIIPVDSEHSAIFQCLEGRRGEEIR
                     EILLTASGGPFFSFEKARLVEAGPEDALKHPTWRMGPKITIDSATMMNKGLEVIEAHH
                     LFGVALEDVRVVVHRQSVVHGGAFFEDGSVVLHAALPDMRLPIAFGLLHPERVDVGAD
                     PVPFDGVNWSFEEPRHDVFRCLPLAVEAGRAGGAYPVALNAANEVAVKAFLDGRIRFL
                     RIADVIEEVLEVVPGFGMMQSMEAIEAVDGWARAEAERRVGGVPR"
     gene            complement(1557..2327)
                     /locus_tag="VFQ10_10305"
     CDS             complement(1557..2327)
                     /locus_tag="VFQ10_10305"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET9965777.1"
                     /translation="MLRWRAATALVLAPIALASIAIGKWAMIAVLLVVIAGAAYELSR
                     ALEPLPFVAALGAGALPLLLSIPYHETGVLAGTLVSLPWALLWLAAKPETRTLRAVLA
                     VLLMSLWVGVPLAHLALFPRSHYGVVLILIAVVGPWISDSGAYFAGRFFGRNLLFPTL
                     SPNKTVEGSVGGLLLTVLIVAPVALQLLDFTIAKALVMGAGVSVSSQCGDLFESSLKR
                     ILDVKDLGNFLPGHGGILDRVDSLLFTAPAVYYISLLV"
     gene            complement(2335..3003)
                     /gene="uppS"
                     /locus_tag="VFQ10_10310"
     CDS             complement(2335..3003)
                     /gene="uppS"
                     /locus_tag="VFQ10_10310"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET9965778.1"
                     /translation="MAIIMDGNGRWAKRRWLPRAAGHRAGVTVLTPLLETAGRAGVKT
                     LTLYAFSTENWARPQREVRTLMKLFLDTAREKVPELNERGARLRFLGRREHLPASVLE
                     AMEEAEVLTSGNDSLDVYIALDYGGRAEIVDATRRIVADGLSPEDVDEMTFASYLYAP
                     DVPEIDLLIRTSGELRVSNFLLWQIAYAEFYVTETLWPDFSPQEFLQALESFGSRSRR
                     RGSV"
     gene            complement(3085..3645)
                     /gene="frr"
                     /locus_tag="VFQ10_10315"
     CDS             complement(3085..3645)
                     /gene="frr"
                     /locus_tag="VFQ10_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET9965779.1"
                     /translation="MSDVIDLTAAGRRMKGAVESVRSNFATIRTGRAKPSLLDRIEVE
                     VYGSRMPLRSVANIGAPEARLLTVTPFDPNSLKDIERAIRDADIGLNPQNDGKILRLP
                     IPELTEERRRDLIRMARSMAEEGRVAVRNVRRDEMKDLHELRKEGEISQDDEHRAEDE
                     LQKLTDGYIKRVEAILADKEAELSEV"
     gene            complement(3656..4438)
                     /gene="pyrH"
                     /locus_tag="VFQ10_10320"
     CDS             complement(3656..4438)
                     /gene="pyrH"
                     /locus_tag="VFQ10_10320"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET9965780.1"
                     /translation="MDESAKLRDESDGTALPQLGPVKRVVLKLSGESLAGDGSGYGID
                     PASLDSTARGVLEAVEAGAELAVVVGGGNIFRGSHVADELGIEGATADYMAMLGTVIN
                     ALALQAALEGRGVPTRVQSAIEIQEVAEPYIRRRAIRHLEKGRVVIFASGTGNPFFTT
                     DTGAALRALEINADALLMAKNRVDGIYDKDPRRHPDAVRLPRLNYMDLLSNDLSVMDH
                     TAATLCGGENLPIVVFDILKSGNLRRILQGEQVGSLVWGAAN"
     gene            complement(4447..5034)
                     /gene="tsf"
                     /locus_tag="VFQ10_10325"
     CDS             complement(4447..5034)
                     /gene="tsf"
                     /locus_tag="VFQ10_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET9965781.1"
                     /translation="MASTIEKIKQLREETAAGMMDVKRALEESEGDVEGARKVLRERG
                     QALAAKKSGRAATEGLIEAYVHFNGRVGVLVEVNCETDFVARTPEFKEFARNVALHVA
                     SMRPMCVSPEDIPAGAVEEERQVVEKQVAEMGKPENISQQILEGRMSKWVAEQALLTQ
                     AYVKEPDKTVGDLLQETIQKVGENVVIRRFVHYEL"
     gene            complement(5038..6084)
                     /gene="rpsB"
                     /locus_tag="VFQ10_10330"
     CDS             complement(5038..6084)
                     /gene="rpsB"
                     /locus_tag="VFQ10_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET9965782.1"
                     /translation="MQSTTERVGIRELLEAGVHFGHQAKRWNPKMKRYIFAQRAGVHI
                     IDLDQTLTLLERALDFVRGVAEANREVLFVGTKKQAQIPLAEEAIRSGQPFVAERYIG
                     GMLTNFQTILPRIQYFKDLAVRVDETPEEERTGKDWFALNREYQKLRRNFAGITEMER
                     LPGGVFVIDPKREELLVKEANRLKIPVLALTDTNCDPEVIDYVIPGNDDAIRSISLIS
                     RLVADAIIAGRGEEAQVEQRPVPPVVEDSQVDGEVEQTGTAAGPRAEEPEATSGPEAA
                     PEAATADESQIEPEAAIQAEGASEPEAVEAEAIDAEADEPEVVAEPQAEETEESGPVE
                     EEAPGEENQETEEK"
     gene            complement(6272..7114)
                     /locus_tag="VFQ10_10335"
     CDS             complement(6272..7114)
                     /locus_tag="VFQ10_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HET9965783.1"
                     /translation="MTRRTLRSLWSQYLRVRKRLAEGDMSGVERRLAEREARGLRDRL
                     VVNYSPLVKYVAGRISGRMAGPLDQEDVVASGLFGLLNAVETYDPERRTKFESYAISK
                     IRWSILDELRKVDPLSRRTRMRAHQIERTRDELVQKLGRAPTESELVGRLGISVTEHR
                     AFLNQYARAQVRSLEARLEYQGGTAAGLHDLIGDHSATDPASAAEMAEVRTRLVRAIE
                     GLGEQERVVTTFYYYEGLTLREIGRVLDLTEGRISQILHSALARLKQSLAEESRLSER
                     IQGR"
     gene            complement(7111..7713)
                     /locus_tag="VFQ10_10340"
     CDS             complement(7111..7713)
                     /locus_tag="VFQ10_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET9965784.1"
                     /translation="MYSDGYTIVYPRRNRGLFQAVRRHGALISEYYLGEAPLAWRFPA
                     RNRIIAGLAGTVVVVEAPEHSGALITARHALDAGRDVWAVPGPPGVRECRGSNGLLAD
                     GAGVLWDIPEFVDAMARERRVPVTKDGASGGAQPLPAGLEDTEAAVLCRVGFEPRGVD
                     EVAKKSGVTMPEVLTALAMLELKGFVSRDGVGSYVRRTVP"
     gene            complement(7632..>8010)
                     /locus_tag="VFQ10_10345"
     CDS             complement(7632..>8010)
                     /locus_tag="VFQ10_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965785.1"
                     /translation="MAAEGHITFDELGAKLRELEGQRTTAEEELDNLQLRRARLEDLE
                     RDRETLLKEYAGMVPEALDELSGEERHQIYRMLHLQVFVYPNGDLEVRGVLREAVCTP
                     MDTPSYTQDAIGASSKRSDATEP"
BASE COUNT         1358 a         2653 c         2422 g         1577 t
ORIGIN      
        1 tcctgcgcca tcgatcctcg ttctacagca ctcacgaccg gttttacgtc ggtgacaaca
       61 ccctggaaca tgtaaacgca ggccagtatg gcgacggccg cgaggaggtt ggccccaggc
      121 ccagcaagaa tgataagggc ccttcgccac aacgctttgg cgtaataggt gtcacggccc
      181 tccgccccat cccccatccc cgccatcttg gcaaatccac ccagaagtat tatgcggaaa
      241 gagtagacgg tgttgccgaa tttcttcttt acgagcgctg gtccgaagcc gacgccgaac
      301 tcggggaccc tgacgcccag ggctttggcc gtgagcatat gccctagctc gtggatgact
      361 atgaggaaga tcagacctag cagcgccacg atgacggtca tcgcggtact cctcccacgc
      421 gtctttcggc ctcagcacgc gcccatccgt ccacggcttc tatggcttcc atgctctgca
      481 tcatcccgaa cccaggcact acctcgagga cctcctcgat cacatctgct atacgtagga
      541 atcttatcct gccgtcaagg aaggccttga cagcgacctc gttggccgcg ttgagcgcga
      601 cgggataggc gcccccggca cgaccggcct ccacggcgag cggcagacac ctgaatacgt
      661 cgtggcgcgg ctcctcgaag ctccaattca ccccgtcgaa gggtacagga tcggccccga
      721 cgtcgaccct ctcggggtgg aggagaccga aggctatggg cagcctcatg tcaggcaacg
      781 cggcgtgcag cacgacggag ccatcctcga agaaagcacc cccatggacc acagactggc
      841 ggtgcacgac gacccgcacg tcctccaacg caacgccgaa gaggtggtgc gcctctatca
      901 cctcgagacc cttgttcatc atggtggccg agtctatggt gatcttgggc cccatccgcc
      961 acgtggggtg ctttagcgca tcctcgggcc cagcctcgac gagccttgcc ttttcgaaag
     1021 agaagaatgg gcctcccgac gccgtcaaca atatttccct gatctcctca ccccgtctcc
     1081 cctccagaca ctggaagatc gccgagtgct ccgagtctac tggtattatg cggttacttg
     1141 ccgcgaggtc cataacccac tcgcctcctg cgacgagcga ttctttgttg gctagggcca
     1201 cccggttgcc ctcctgcagg gcgccgacgg tggcagcgag gcctgcgaag ccgactatcc
     1261 cgttaagcac gacgtctgat ggcgcgcggg agagttgctc ggccgagccg ggaccggcga
     1321 agacttcgga ggcgagatcc tcgaggggtc ctgcttcgct tgattccagc gccacatact
     1381 caggtcggaa ctcggccgcc tgaagggcga gcgcccgagc atcgttaccc gcagagagtc
     1441 cgacgatttc gaagcgttca ggatatctac tgacgacgtc gagggcttgc cttccgatac
     1501 tgcccgtcga gccgaggatc gtgaggcgac gtctcatgct tgtgttgggt ccgatttcat
     1561 acgagtaggg agatatagta taccgccggg gccgtgaaga gcaggctatc cacccggtcg
     1621 aggatgcctc cgtggcctgg gaggaagtta ccgaggtcct tcacgtcgag gatacgcttc
     1681 aaggacgatt cgaagaggtc tccgcactgg ctcgagacag agacaccggc gcccattacg
     1741 agggccttgg cgatcgtaaa gtcgaggagc tgcagggcaa cgggggcgac gatcaggacc
     1801 gtcaagagga ggccgccaac gctgccctcc acggttttgt tcgggctcag ggtcggaaac
     1861 agcaggttac ggccgaagaa gcgcccggcg aagtacgcgc cagagtcgga gatccagggc
     1921 cccaccaccg ctatcaggat gaggacgacg ccgtaatggg agcggggaaa cagcgccaga
     1981 tgcgccagcg ggacgccgac ccagagcgac atgaggagca cggcgagaac agcgcgcagg
     2041 gtcctcgtct ctggtttggc ggcgagccag agcagggccc agggcaggct cacgagggtg
     2101 cccgccagta cacccgtctc gtggtaaggg atggagagta gaagtgggag cgcgccggcg
     2161 ccgagggccg ccacgaaagg cagaggctcg agcgcccgcg agagctcgta cgcggccccc
     2221 gctatgacca cgagtagtac agcgatcatg gcccatttgc cgatggctat gctggccagc
     2281 gcgatcggcg cgagcaccag ggcggtcgct gccctccacc ggagcacagg gaccctagac
     2341 gctgccccgg cgcctggagc gggacccgaa agactccaga gcctgcagga actcctgggg
     2401 ggagaagtcg ggccacagcg tttccgtcac gtagaactcg gcgtaggcta tctgccagag
     2461 caagaagtta gagacgcgca gctcgccgct ggtccggatc agcaggtcga tctcgggtac
     2521 gtcgggggcg tagaggtaac tggcgaacgt catctcgtcg acgtcctcag gagagagacc
     2581 gtctgcgact atccgccggg tggcgtccac gatctccgcg cgtcctccat agtcgagcgc
     2641 gatgtagacg tcgaggctgt cgttacccga ggtgaggacc tcagcctcct ccattgcttc
     2701 cagcacagag gcaggcagat gctcccttct gcccagaaaa cggagcctgg caccgcgctc
     2761 gttcaactcc ggcactttct cacgggccgt gtcgaggaag agcttcatga gtgtccttac
     2821 ctctcgctgt ggacgtgccc agttctcggt cgagaaggca tagagggtaa gcgtcttcac
     2881 ccctgcgcgt ccagcggtct caagcagggg agtgagcacc gtgacaccgg ccctgtggcc
     2941 tgccgcgcgc ggaagccagc gtcgcttggc ccagcgcccg ttgccgtcca taatgatggc
     3001 cacgtgacgc ggagtccggt ctgggtcgag gacgcgctgc tcgggggaat ctgtggggag
     3061 tcgtttctcg aggcgttcca gaagttagac ctccgagagc tcggcttcct tgtcggcgag
     3121 gatagcctcg actctcttga tgtagccgtc tgtgagcttc tgtagttcgt cctccgccct
     3181 gtgttcgtcg tcctgggaga tctcgccttc cttgcgcagc tcgtgcagat ccttcatctc
     3241 gtcgcggcgc acgtttctca cggcgaccct accctcctcg gccatgctcc tcgccatcct
     3301 gatcaggtcc cggcggcgct cctccgtcag ttcagggatg ggaagcctca gtatcttgcc
     3361 gtcgttctgc gggttgaggc cgatatctgc gtcccttatg gcacgttcga tgtccttcag
     3421 ggagttaggg tcgaaggggg tcacggtgag gaggcgtgcc tccggcgcac cgatgttggc
     3481 gacgcttctc aggggcatcc tcgatccgta gacctcgacc tcgatcctgt ccaatagtga
     3541 gggtttagcc ctgcctgtgc gtatcgtggc gaaattgctc cgaacagact cgacggcccc
     3601 cttcatcctg cgtccggccg ccgtgagatc aatgacgtca gacatctcat cctcctcagt
     3661 tcgcggcgcc ccacaccagc gaccctacct gttccccctg caagatacgg cgcaggttgc
     3721 ctgacttgag tatatcgaaa acgactatgg gcagattctc gccgccgcac agcgtggccg
     3781 ccgtatggtc catgacggac aaatcgttgg agagcaaatc catgtagttg agcctgggca
     3841 ggcgcacggc gtcgggatga cgccttgggt ccttgtcgta gattccgtcc accctgttct
     3901 tggccatgag cagggcgtcg gcgttgatct cgagcgcccg cagcgccgcc cccgtgtcgg
     3961 tggtgaagaa agggttgcct gtccccgagg cgaagatcac gacgcggccc ttctccaggt
     4021 gcctgatggc acgcctgcgg atgtagggct cggcgacctc ctggatctcg atggcggatt
     4081 ggaccctggt gggcacgcct cgtccttcga gggcagcctg caaagccaac gcgttgatta
     4141 cggtgccgag catcgccatg tagtcggccg tggccccttc tattcctagc tcgtcggcga
     4201 cgtgggaacc acggaagatg ttgccgccgc ccacgacgac ggccagctcg gcccccgcct
     4261 ccacagcctc gaggacgccc cttgccgtcg agtcaaggct agcagggtcg atcccgtagc
     4321 cgctaccatc gccggcgagg ctctcccccg aaagcttcag gacaacgcgc tttacggggc
     4381 cgagctgtgg gagcgcggtc ccgtcggact cgtccctgag tttggcggac tcatccaagt
     4441 ttgctactag agctcgtagt ggacgaagcg ccggatcacg acgttctcgc ctaccttctg
     4501 gatcgtctcc tgcaagagat cgccgaccgt cttgtcaggc tctttgacat aggcctgcgt
     4561 gaggagggcc tgctcggcga cccatttact catcctgccc tcgaggatct gctgcgagat
     4621 gttctcgggc ttgcccatct cggcgacctg cttctcgaca acctgccgct cttcttctac
     4681 ggccccggcg gggatgtcct caggcgagac gcacatcggc cgcatggagg ccacgtggag
     4741 cgccacgttg cgggcgaact ccttgaattc gggcgtgcgc gccacgaaat ccgtctcgca
     4801 gttgacctcg acgaggacgc caacccgtcc gttgaagtgc acgtaagcct cgatgaggcc
     4861 ctcggtcgca gccctcccgc tcttctttgc ggccagggcc tggcccctct ccctgaggac
     4921 cttcctcgcg ccttcgacgt ccccttcgga ctcctcgagc gcccgcttca cgtccatcat
     4981 ccccgcggca gtctcctccc tgagctgctt gatcttctct atcgtgctag ccaaatccta
     5041 cttctcctca gtctcttggt tctcctcgcc cggcgcctcc tcttcgacag gcccggactc
     5101 ttctgtctct tctgcctgag gctctgcaac cacctcaggt tcgtcggcct ccgcatcgat
     5161 ggcttcggcc tcgacggcct caggctctga agcaccttcg gcctgtatcg ccgcctccgg
     5221 ttcgatctgg gactcgtctg ccgttgccgc ctcgggtgca gcttcgggtc ctgaggtggc
     5281 ttctggctcc tccgctcgtg gccctgcggc cgtccctgtc tgctctacct cgccgtccac
     5341 ctgagagtcc tcgaccaccg gcggcacggg tcgttgctcg acctgcgctt cctcccccct
     5401 gccggcgatg atcgcatcgg cgacgagcct ggagatcagg ctgatcgaac gtatcgcgtc
     5461 gtcgttgcct gggatcacgt agtcgatgac ctcggggtcg cagttggtat cggtgagggc
     5521 cagcacaggg atcttgagcc tgttggcctc tttgaccagg agctcctcgc gcttggggtc
     5581 tatgacgaac acaccgccgg ggagcctttc catctccgtt atgccggcga agttgcgcct
     5641 tagtttctgg tactccctgt tcagggcgaa ccagtccttg cctgtccttt cctcctcggg
     5701 cgtctcgtcg accctgacgg ccaggtcttt gaagtactgg atgcgcggaa ggatcgtctg
     5761 gaagttggtg agcatgccgc ctatgtagcg ctccgcgacg aagggttgcc cactcctgat
     5821 ggcctcctcg gcgagcggga tctgggcctg cttcttagtc cctacgaaaa ggacctcccg
     5881 gttcgcctca gcgacaccgc gaacaaagtc caacgcccgc tccaacaggg tcagcgtctg
     5941 atcaaggtcg ataatatgaa ccccggcccg ctgggcgaag atgtagcgct tcatcttggg
     6001 gttccaccgc ttggcttggt gtccgaaatg gacgccggct tcgagcagtt ctcggattcc
     6061 tacgcgctcg gttgtgctct gcaagatctc tacctctctt ttaaggtggt tggtatcctc
     6121 cgcccgcgcc tcgcaatccg cccttggatc tcccggcgga ccaccacccg ggtccggagg
     6181 accctgcggc gggcgtgcgt agttgtagac gagacctgaa agcatagcct cgtccgaccc
     6241 cgcagatgtt agcacaaggg ggtggatgct tctagcgccc ctggattctt tcggagagcc
     6301 ggctctcctc ggcgaggctt tgcttgagcc gcgcgagcgc gctgtgcagg atctgcgaga
     6361 tacggccttc ggtcaggtcg aggacccttc ctatctccct cagcgtaagg ccttcgtaat
     6421 agtagaaggt ggtgactaca cgctcttgct cgccgaggcc ctctatggcc cgaactagcc
     6481 gcgtgcgaac ctcggccatc tccgcagccg acgcaggatc ggtcgccgag tggtccccga
     6541 tgaggtcgtg cagcccggcg gcagtgcctc cctgatactc caggcgggct tcgagcgacc
     6601 tcacctgcgc cctcgcgtac tggttgagga acgctcgatg ttcggtgacg ctgattccca
     6661 ggcgcccgac caactcagac tccgtcggcg cgcggcctag cttctgcacg agttcgtccc
     6721 tggtacgctc gatctggtgc gccctcatgc gagtccggcg agacagtgga tctaccttcc
     6781 tcagctcatc gagtatggac caccgaatct tggagatcgc ataagactcg aacttggtgc
     6841 ggcgctctgg atcgtaggtc tcgacggcgt tcagcagccc gaagagaccc gaagcgacga
     6901 cgtcctcctg atcgagcggt ccggccatgc gccccgagat tctgccagcg acgtatttca
     6961 cgagcgggga gtagttgacc acgagccggt ctctcagtcc cctggcctca cgttcggcca
     7021 accggcgctc gacgcccgac atatcccctt cggccaggcg ctttcggacc cgtagatatt
     7081 gcgaccacag agagcggagc gtgcgccgcg tcacgggacg gtcctcctca cgtaagatcc
     7141 aacaccgtca cggctcacga accccttgag ctcgagcatg gcgagcgcgg taagcacctc
     7201 gggcatcgta acgccactct tcttcgcgac ctcgtcgacc cccctcggtt cgaagccgac
     7261 cctgcagagc acagcagcct ccgtgtcctc cagtccagca ggcaagggct gggcgcctcc
     7321 ggatgccccg tccttcgtca cggggactct gcgctcccgc gccatggcat cgacgaactc
     7381 tggtatatcc cacagaacgc ctgccccgtc agcgagcagc ccgttcgagc ccctgcattc
     7441 gcgcacgcct ggcgggccag gcacggccca tacatcgcgt cctgcgtcga gggcgtggcg
     7501 ggctgttatg agggccccgc tatgctcggg cgcctccacg accactacgg tcccggcaag
     7561 gccggctatt atgcggtttc tggccgggaa tcgccatgcc aggggcgcct cgcccaggta
     7621 gtattcggag atcagggctc cgtggcgtct gaccgcttgg aagaggcccc tattgcgtct
     7681 tgggtatacg atggtgtatc catcggagta caaacggctt cccgcagcac cccgcgcact
     7741 tcgaggtcgc cgttagggta gacgaacacc tgtaggtgga gcatccgata gatctggtgg
     7801 cgctcctcgc cgctcagctc gtccagggcc tctggcacca tgcccgcgta ctcctttagg
     7861 agggtttccc tgtcgcgctc caggtcctca aggcgggcgc ggcgcaactg caggttatct
     7921 agctcctcct ctgccgtggt gcgctgtccc tccagctccc ggagctttgc gcccagctcg
     7981 tcgaacgtga tgtgaccctc cgcagccatg
//
LOCUS       DASTKM010000253         3852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_262769, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000253 DASTKM010000000
VERSION     DASTKM010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262769"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..695)
                     /locus_tag="VFQ10_10350"
     CDS             complement(204..695)
                     /locus_tag="VFQ10_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965786.1"
                     /translation="MNRRGLWAGGVALILLVVVVGAAALLTTVGAGYAKDEDASSRAK
                     CSVATLEGRYLFAQNGVEIKGNDQRPFAIAGYDVFDGNGEVKGVASGNFNGETFRNDR
                     FSGTYSVKANCTGTVTFKDGAATQGDVFIAPDGSMFAFVRTTPEDVAAGIDPQVTAKR
                     VGE"
     gene            complement(1124..2152)
                     /locus_tag="VFQ10_10355"
     CDS             complement(1124..2152)
                     /locus_tag="VFQ10_10355"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9965787.1"
                     /translation="MMIVEDRCTILQIWETGDAGTSEEAAMELMNRPPSEREARRAQA
                     DRDELAERVARAVGEDGAVEAPGGLRLLRQSSPTPKEHGVSSPAFCVIARGSKEVLLG
                     DDCYRYDADRYLITAAALPTATRVTGASEERPYLGVVLELDPALVGSVMVEAGHPAPR
                     EHAAARAFEVSPLDAGLLDAVVRLVGLLDSPAEEARFLRPLVTREIVFRLLKGEQGDR
                     LRQIAVLGGHSHRIARALERLRKDFDRPLRIEDIARELGMSVSGFHHHFKAVTAMSPL
                     QFQKRMRLQEARHLMLAEDLDAASAGYRVGYSDASHFNREYKTLFGAPPMRDVERLRE
                     GARESAGL"
     gene            2312..3082
                     /locus_tag="VFQ10_10360"
     CDS             2312..3082
                     /locus_tag="VFQ10_10360"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9965788.1"
                     /translation="MAANQNGSYAGKVAFVTGAGSGIGRATALAFAREGASVVVADAS
                     EQGNRKTAHMIEELGGRALAVRCDVTRAGDVRSVLDRTIETFGHLDVAFNNAGVEQEE
                     AATGEIAEEEWERVVDTNLRGVFLCMKHEIPLMLERGGGTIVNTSSGAGVKGFPRGAA
                     YVAAKHGVIGLTKAAALDYAQANIRVNAVCPGIIDTEMMDRVTGDTLEGLQRVISQEP
                     IGRMGKPEEIAAAVLWLCSEAASFVVGHAMVVDGGQTV"
     gene            3205..>3852
                     /locus_tag="VFQ10_10365"
     CDS             3205..>3852
                     /locus_tag="VFQ10_10365"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9965789.1"
                     /translation="MQGTVLYGPRDVRFEERPDPTIVEQTDAVIRLPLTCVCGSDLWP
                     YRGIEPIDESTPMGHEYVGIVEEVGSEVRLIEPGQFVVGSFFASDNTCEICQAGYQSS
                     CIHLQPGAPTDAQAEYARIPLADGTLVATPGLPPEDLIPSLLAASDVLGTGWFGAVAA
                     EAGPGKTVAVVGDGAVGLLAVLAANQLGAERIIAMSRHESRQRLAREFGATEIVTE"
BASE COUNT          691 a         1217 c         1277 g          667 t
ORIGIN      
        1 ggcgggaccc gttggccccg cctcaagttt atcaacgtgc ccccagtgtg aggggttaga
       61 agggctgacg caacttccga gaattcgtaa caggcgaagt tcgcagaatt agccttccac
      121 gcactcgggt ggataagggc aaagagcaag ggcggaggat ttgcctcccc cactagctgc
      181 tccgcccagg ctcccttagc ggttcactct cccactcgct tggccgtcac ctgcgggtca
      241 atccccgccg ccacatcctc gggggtggta cgcacgaacg cgaacataga gccatcaggg
      301 gcgatgaaca catcgccctg cgtagcggca ccatccttaa aggttaccgt gcctgtgcaa
      361 ttcgctttga cgctatacgt gccagagaag cgatcgttac gaaaagtctc cccgttaaag
      421 ttacctgaag cgacaccttt cacctcaccg ttgccgtcaa agacatcata gcctgcaatg
      481 gcgaacggcc tctgatcatt gcccttgatt tcaaccccat tctgggcgaa cagatacctc
      541 ccctccagcg tggccacaga gcacttcgcc ctgctggagg cgtcctcatc cttggcatat
      601 cccgctccaa cagtagttag tagggctgcc gcgcctacga cgacgaccag taggatcagt
      661 gcgacacccc ctgcccatag ccctctacgg ttcattgtgg tcctcctcct cgtgtgttgt
      721 cgcccacaga gagtcctccc ctcctcttct gtggggctca ttcccaggag acgtagtaac
      781 aggccacaat gaatcggcga tgaatttgcg cggcgaaaca ctaaggtaag ggtgttcggg
      841 gctagagaag tgtcccggcc tagccccggt ccgttcggcc taggggttgc cctgctgtag
      901 cgtggcccca gaagccatac tctcgcgcct ctcagagggg gatctatgca ctatcctgag
      961 tccgaacttc ggagaattca ctttccacgc actccggtga ataacgtgtc aacgttatgg
     1021 gaaaaacggg ctcatggcct gatttgagga ggatcgtccg ctaaggacgg tgctgacgcg
     1081 tcggtggcga atcgggcagg ccgatcggca accggtgatc ggctcacagg cctgcactct
     1141 ccctggcgcc ttcccgcagc cgttccacgt cgcgcatagg cggcgcgccg aagagcgtct
     1201 tgtactcgcg gttgaagtgc gaggcgtcag agtagcccac tcggtagccg gcgctcgcgg
     1261 cgtcgagatc ctcggctagc attagatggc gggcctcctg gaggcgcatc cgcttctgga
     1321 actgcagggg gctcatcgcg gtgaccgcct tgaagtggtg gtggaatccc gagacgctca
     1381 tccctagctc cctggcgatg tcttcgatcc ggagcggcct gtcgaagtcc ttgcggagcc
     1441 gctccagggc ccttgcgatg cggtggctgt ggccgccgag gaccgcgatc tgacgaagcc
     1501 ggtcgccctg ctcccccttt aggagccgga agacgatctc ccgggtgacg agcggccgga
     1561 gaaagcgggc ctcctcggcg ggcgagtcca ggagcccgac gagacgcacc acggcgtcca
     1621 agaggcccgc gtccagcggg ctcacctcga aggcccgcgc ggctgcgtgc tcccgcggcg
     1681 cggggtggcc ggcctcgacc atgaccgagc cgacgagggc ggggtcgagc tcgaggacga
     1741 cgcccaggta tggccgctcc tcggacgccc cggtgacccg ggtcgcggtg ggcagcgcgg
     1801 cggcggtgat gaggtagcgg tcggcgtcgt agcggtagca atcgtcgcct agcaggacct
     1861 ctttggagcc ccgcgcgatc acgcaaaagg cgggtgagga gacaccgtgt tccttcgggg
     1921 taggcgagga ctggcggagc agccggagcc cccccggcgc ctcgacggcc ccgtcctcgc
     1981 cgacggcccg agcgacgcgc tcggccagct cgtcgcggtc ggcttgcgct ctgcgcgcct
     2041 ctcgctctga cggtgggcgg ttcatcaatt ccatggcagc ctcttcgctc gttcccgcat
     2101 cacccgtctc ccagatttgc aggatagtac aacgatcctc gacgatcatc ataccggctg
     2161 cgctccaggg ctcctacaat cggcggcaga cggatgaagt cgatcccgac ccttaggagt
     2221 aatctgggct tttgttcaag aggggcttcg gctttggtag gggatgagac ctaactactg
     2281 acgacacgat tagcaacggg gataggagaa catggcagcg aatcagaacg gaagctacgc
     2341 gggaaaagtt gcgtttgtaa ccggggcggg gagtggcatc ggccgtgcga cggcgctggc
     2401 gttcgcgcgc gagggcgcca gcgtggtggt cgccgacgcg tcggagcagg gcaaccggaa
     2461 aacggcgcac atgatcgagg aacttggcgg acgggcactt gccgtcaggt gcgacgtgac
     2521 gcgggccggg gatgtgagaa gtgttctgga caggactatc gagaccttcg gacatcttga
     2581 cgtcgccttc aacaacgcgg gggtcgaaca ggaggaagca gccaccgggg aaatcgcgga
     2641 ggaggagtgg gaaagggtcg ttgacactaa ccttcgcggc gtctttcttt gcatgaagca
     2701 cgaaatcccc ctgatgctcg agcgaggggg cggcacgatc gtgaacacct cctcgggggc
     2761 cggggtcaaa ggcttcccga ggggggccgc ctacgtcgct gcgaagcacg gcgtgatcgg
     2821 cctgaccaag gcggcggccc tggactacgc gcaggcgaac atccgtgtca acgccgtgtg
     2881 tcccgggatc atcgacaccg agatgatgga ccgggtcacc ggtgacacgc tggaaggact
     2941 ccagcgggtg atctcgcagg agcccatcgg gaggatggga aagccggagg agatcgccgc
     3001 ggccgtcctg tggttgtgtt cggaggcggc ttccttcgtc gtagggcacg cgatggtcgt
     3061 cgatggcggc caaacggtgt agtgatgttc gcccccaacc gtgacgccgc tgcggtgaac
     3121 gcgacgggcg tggagaccgc gtcaggcggc gcggcgtacg ttgaagacgc gaccaagtac
     3181 tcgatattcg gaaggagata aatcgtgcaa ggaacagtac tttatggccc ccgggacgtg
     3241 cgcttcgagg agcgccccga cccgacgatc gtcgagcaga ccgacgccgt catcagattg
     3301 ccgctcacct gcgtgtgcgg ctcggacctc tggccttacc gcggcatcga gccgatcgac
     3361 gagtcgaccc cgatgggcca tgagtacgtc ggcatcgtcg aggaggtcgg cagcgaggta
     3421 cgtctgatcg agccgggcca gttcgtcgtc ggctcgttct tcgcgtcgga caacacctgc
     3481 gagatctgcc aggccggcta ccagtcgtcc tgcatccacc tgcagcccgg tgcccccacc
     3541 gacgcacagg cggagtacgc ccgcatcccg ctcgccgatg gcaccctcgt agcgacgccg
     3601 ggcctgccgc cggaggacct gatccccagc ctgctcgcgg cctccgacgt gctcggcacc
     3661 ggctggtttg gcgccgtggc ggccgaggcc ggtccaggca agacggtggc agtcgtcggc
     3721 gacggcgccg tcggcctgct cgcggtgctc gccgccaatc agctcggcgc ggagcggatc
     3781 atcgcgatga gccggcacga gtcccgccag cggctcgccc gggagttcgg cgcgaccgag
     3841 atcgtgaccg ag
//
LOCUS       DASTKM010000254         4943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_614073, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000254 DASTKM010000000
VERSION     DASTKM010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614073"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="purM"
                     /locus_tag="VFQ10_10370"
     CDS             <1..742
                     /gene="purM"
                     /locus_tag="VFQ10_10370"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET9965790.1"
                     /translation="ATGARPLFFLDYVASGRLDPEAVVGIVSGAAEACGRLGVALIGG
                     ETAEMPGIYGQGDFEVVGVCVGACERSEVVSGERVRPGDAVLGLASSGLHTNGYTLAR
                     KVLAEAGVSYDDVPEGLDRSIGEIYLEPHRAYVRKIEALRGVVDVRGMAHVTGGGLVG
                     NLPRALGGLGARLYAGSWSEPAVFGLIRSLGGLPESEMRGVFNLGVGFCALVPPEGAT
                     AGLEALRGEGCDAWRIGEVVEAGGLEFV"
     gene            735..1316
                     /gene="purN"
                     /locus_tag="VFQ10_10375"
     CDS             735..1316
                     /gene="purN"
                     /locus_tag="VFQ10_10375"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET9965791.1"
                     /translation="MSERLPEGSRFAVLASGSGTNLQALLDAYPENLVVVAGDNKEAF
                     AFERARRSGVPVEHVDPAGFVGKEDYDRELAGRIEAHDVGLVVGAGYMRILSAGFLDR
                     FPAVLNVHPSLLPEFRGLRAVQQALEAGVEETGVTVHFMVEEVDAGPIVAQQRVPVLP
                     EDTKETLLGRLHPVEHRLLVRAVADYFLGRVEV"
     gene            1313..2851
                     /gene="purH"
                     /locus_tag="VFQ10_10380"
     CDS             1313..2851
                     /gene="purH"
                     /locus_tag="VFQ10_10380"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET9965792.1"
                     /translation="MKRRALISVSDKRGIEDFARRLSAMGFEIISTGGTARTLTEAGV
                     RVVPVSEVTGAPEILGGRVKTLHPKIHGGILADLGDPDHVEQLVEQDIGPIDLVCINL
                     YPFEETVAGGAPEKEVIEQIDIGGPAMLRAAAKNFGSVIVVPSPEVYEEVLAALESEG
                     QVPEETRRRLALAAFRRTAGYDAAIAGWLSEQVEGDAEGFPERRTVRYERVSGLRYGE
                     NPHQAAAYYAEVGAEHLMSGVEMLQGREISFNNLYDLDAARTLLADLSDLGTPAAVIV
                     KHANPCGAAVGDSLMDAYRKALASDPQSAFGGIVALSGEVDRDLAAEISSIFTEILVA
                     PGFTAKAREILSTKPNVRVLVAGLLDRPALSSKYVTGGVLLQASDAVEDDSEYRVVTE
                     ARPSPQQMGDLIFAWRVARVVKSNAIVLARDGATVGVGAGQMSRVDSSDIAVKKAGEK
                     AQGAAAASDAFFPFADGVEALAEAGIEAVIQPGGSVRDEEVVEAANRHGLAMVFTGRR
                     HFLH"
     gene            3103..3312
                     /locus_tag="VFQ10_10385"
     CDS             3103..3312
                     /locus_tag="VFQ10_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965793.1"
                     /translation="MNSHEARVGTLVGLLDGVKRTGKGRIGTIVRIYGDPDDLAMDVR
                     FEDGSVALYWYDELRRMEESLQEAG"
     gene            3318..3512
                     /locus_tag="VFQ10_10390"
     CDS             3318..3512
                     /locus_tag="VFQ10_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965794.1"
                     /translation="MQAYEARRGTVVRVREGHWKSQFAGMRGTVQNLWGQSEYAAVDV
                     LLEDGRSELFWLPDLADIAV"
     gene            4127..4198
                     /locus_tag="VFQ10_10395"
     tRNA            4127..4198
                     /locus_tag="VFQ10_10395"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4159..4161,aa:Lys,seq:ttt)
     gene            4300..4372
                     /locus_tag="VFQ10_10400"
     tRNA            4300..4372
                     /locus_tag="VFQ10_10400"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4334..4336,aa:Glu,seq:ttc)
     gene            4444..4515
                     /locus_tag="VFQ10_10405"
     tRNA            4444..4515
                     /locus_tag="VFQ10_10405"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4476..4478,aa:Val,seq:tac)
     gene            complement(4617..>4943)
                     /locus_tag="VFQ10_10410"
     CDS             complement(4617..>4943)
                     /locus_tag="VFQ10_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET9965795.1"
                     /translation="QMEEMERLGDIYIDQGLVFTTEVGTLINPSNLRQRSFKPLLERA
                     GLPRITFNDLRHTCASLLFQKNVHPKFVQELLGHASVAITLDTYSHMLPGMGSQAADA
                     MVEALS"
BASE COUNT          904 a         1362 c         1707 g          970 t
ORIGIN      
        1 cgccacgggc gcgcgtccgc tcttcttcct cgactacgtc gccagcggcc gcctggaccc
       61 agaagccgtc gtcggcattg tcagcggcgc cgcagaagcc tgcgggcggc tcggtgtcgc
      121 gctcatcggc ggcgagactg ctgagatgcc aggtatctac ggccagggag acttcgaggt
      181 cgtcggcgtg tgcgtagggg cgtgcgagcg gagcgaggta gtcagcggtg agagggtacg
      241 tccaggcgat gctgtgctcg gccttgcgtc cagcggtttg cacaccaacg gttacacgct
      301 ggcccgcaag gttctcgctg aggcaggcgt ctcctacgac gacgttcctg agggtctgga
      361 ccggtccatt ggagagatct acctcgagcc gcatcgggcc tacgtgcgaa agatagaagc
      421 gctgcgtggg gtggtcgatg ttcgggggat ggcgcacgtg acggggggcg ggctcgtggg
      481 gaaccttcct cgggcgctcg gtgggctggg agcgcggctc tatgcgggat catggtcgga
      541 accggcggtc ttcgggctaa tacgttcact gggcggcttg cccgaaagcg agatgcgcgg
      601 ggtcttcaac ctcggcgttg gtttctgcgc gctcgtgccg cccgaagggg caacggccgg
      661 gctcgaagcg ctgcgcgggg aaggttgcga tgcctggcgg atcggggagg ttgtcgaggc
      721 cgggggccta gagtttgtct gagcggctgc ccgaaggctc gcgcttcgcc gtgctggctt
      781 cgggttctgg gacgaacctg caggcattgc tcgacgcata tcccgagaac cttgttgtcg
      841 tcgcggggga caacaaggag gcgttcgcgt tcgagcgggc gcggcggtcc ggagttcctg
      901 tcgagcacgt cgatcctgcc gggttcgtcg gcaaggaaga ctacgatagg gagcttgcgg
      961 gcaggatcga agctcacgac gtcgggctgg tggtgggggc cgggtacatg cggatactgt
     1021 cggctggctt cctggatcgc ttccctgctg tgttgaacgt tcatccttcg ctcttgcccg
     1081 agttccgcgg gcttcgcgcc gtgcagcagg ccctggaggc gggggtagaa gagacggggg
     1141 tgacggtgca tttcatggtc gaggaagtag acgccgggcc aatcgtcgcg cagcagcggg
     1201 tgccggttct gcctgaagat acgaaggaga cgttgctcgg aaggttgcac cctgtagagc
     1261 acaggttgct cgttcgggct gtcgcagact atttcctggg gagggtggag gtatgaaacg
     1321 gcgggccctg atctcggtat ccgacaagag ggggatcgag gacttcgcgc ggcggctctc
     1381 cgcgatgggg ttcgagatca tctcgaccgg gggaacggcg aggacgctca cggaggccgg
     1441 agtcagggtc gtccccgtct ccgaggtgac gggcgcaccg gagatactcg gcgggcgggt
     1501 caagacgctg caccccaaga tacacggcgg catcctagcc gacctcggag accctgacca
     1561 cgtagagcag ctcgtcgagc aggatatcgg cccgatagac ctggtctgca tcaacctcta
     1621 tcccttcgaa gagaccgtcg ccgggggcgc tcccgagaag gaggtcatcg aacagataga
     1681 catcggcgga ccagcgatgc tgcgggccgc cgcgaagaat ttcgggtccg tgatcgtcgt
     1741 cccctcgccc gaggtctacg aagaggtgct ggcggctctg gaaagcgagg gacaggtccc
     1801 agaggagacc cgccgccgcc tggcgctcgc ggcgttccgg cggaccgccg ggtacgacgc
     1861 ggcgattgct ggttggctca gtgagcaggt cgaaggggat gccgaaggct tccccgagcg
     1921 tcgcacagtc cgatacgagc gagtctcggg gctgcgttac ggggagaacc cgcaccaggc
     1981 cgcggcctac tacgctgagg tgggggccga gcatctaatg agtggcgtgg agatgttgca
     2041 ggggcgggag atctccttca acaacctcta cgacctcgac gccgcccgca ccctcctcgc
     2101 cgacctgagc gatctcggaa cccctgccgc cgttatcgtc aagcacgcca acccttgtgg
     2161 cgccgcggtg ggagactccc tgatggacgc gtaccgcaag gcgctggcct cggacccaca
     2221 gtctgcgttc gggggtatcg tggccctcag tggcgaggta gatcgggatc ttgcggccga
     2281 gatctcaagc atattcacgg agatactggt cgcacccggc ttcaccgcga aggccaggga
     2341 gatcctctcc acaaagccca acgtgagggt gctcgtagca ggcctgttgg atcgacccgc
     2401 actctcatcc aagtacgtaa cgggtggggt cctgctccag gcatcggacg ccgtggagga
     2461 cgactcggag tacagggtcg tcaccgaggc gcgtccttcg ccgcagcaga tgggcgatct
     2521 tatcttcgcc tggcgtgtcg cccgcgtggt caagagcaac gccatagtcc tcgccagaga
     2581 cggggccacg gtcggtgtag gagcgggtca gatgagccgg gtcgactcat cggatatagc
     2641 cgtgaagaag gccggcgaaa aggctcaggg agccgccgca gcgagtgacg ctttcttccc
     2701 tttcgccgac ggcgtagagg cgctggccga agctggcata gaggccgtca tacagcccgg
     2761 cggctccgta cgcgacgaag aggtcgtcga agcggcgaac cgccacgggc ttgcgatggt
     2821 ctttaccggg cgacgccatt tcttgcactg aggctgaata ctactaacag ccctaacaac
     2881 tgtcaggggc tgccggcagg cgccgctgct acttcgtccc accgcctatg caacagttac
     2941 cgatcggtga gaagggggta tcggtgttgg gggacatata gtgtcacagg gtacataaaa
     3001 aggtgtaggg acctgctaca tatgtcgctt gagaaaggga aagcgcatag ggccggagtt
     3061 cgagtctggc cccaagcaca gaacgacaag gaggcgaact tcttgaactc acatgaggcg
     3121 cgtgtcggca cgttggtggg actcctggat ggagtcaagc ggactgggaa ggggcgcatt
     3181 ggcactatag tgagaatcta cggtgatcct gacgacctgg cgatggacgt gcggttcgag
     3241 gatggaagcg ttgcgcttta ctggtacgac gaactgcgaa ggatggagga gtcactacag
     3301 gaggcaggct aaagctcttg caagcttacg aagctcgtag aggcactgtg gtgcgggtga
     3361 gggagggcca ctggaagagc cagtttgccg gtatgagagg cacagttcag aacctctggg
     3421 gccagtcgga atacgcggct gtggatgttc tgctcgagga cgggcgctct gagttgttct
     3481 ggctccccga cctggctgat atcgctgttt agtcgttcgg tcttatgctg gtggggcatg
     3541 agagcacaga cgggcatatc gggttctggt tgccgttcgg gtattacctg gaacgtgatg
     3601 cagaccttgt aatcttgcgt aggtccgacg gttctttcgt ggcagcgttc agtgccgtgg
     3661 gtgcagaccc ctttgaagtg gagttgacgg tgtgggaaga cgcagattag aagcgtgcgg
     3721 tctgcgcgtg ggaattactt ccgcatcgta gaatcaagtg gcagctacaa gaaacctaac
     3781 ctcggtcacg tatggacgtc aaccagcggt ccaggtacgc tcccacggtc agctcttccg
     3841 aatcgtgggc tctgccctcg tcgcgctcct ggagctactt gcgaccggcg actttcactt
     3901 gtgtcttcgt caaggtcttg ctcgtgatgt gtcgcgtctc accgttggcg tcctcgtaga
     3961 cgctgccgac acgaccatca gcacgcctga agacactatc ctctccgttt gccctcttct
     4021 tggtcattct tccggttgcc gtcacggtta ctgtcatggc gtaaatcaag ggttggcgag
     4081 ggccgtgcat attgttgagt tttgctggta gaatcgggca gagcctgggc cagtagctca
     4141 gtggtagagc aggggacttt taatcctcgt gtcgtaggtt cgatccctac ctggctcacg
     4201 tattcttcgg tggcgaatac cccgcctcat atcagggacg atctgttaaa ctctcgactg
     4261 cgaaaccggt agtggctccg gaccctgccc gtctcgtgag cccccatcgt ctagaggccc
     4321 aggacaccgc cctttcacgg cggcggcacg ggttcgaatc ccgttggggg cacttgttag
     4381 taggtcagcg cgcgcgatca gaagcagaaa agctgaagtg ctgattagct gaaatgctct
     4441 aacgggcgat tagctcagtg gtagagcgcc tcccttacaa ggaggaggtc gctggttcga
     4501 acccagcatc gcccactcta ggaaagtcgt ggttttgcag gtaaaatgtg tgagcgggga
     4561 gaaggccccg acgtcacatc ggggcctgtg tgcagcaacg gtgcagcaac gcggagctaa
     4621 ctgagtgctt ccaccatcgc gtcggcggcc tggctgccca tgccggggag catgtgggag
     4681 taggtgtcga gggtgatggc gacggaggcg tgtcctaata gctcctgcac gaacttcggg
     4741 tgtacattct tctgaaatag caacgaggca caggtgtgac gcaggtcatt gaaggtgatt
     4801 cgaggcagac ccgcacgctc taggagcggc ttgaaggatc gctgtcggag gttggacggg
     4861 ttgattaggg taccgacctc ggtggtgaaa acgagaccct gatcgatgta gatgtctcca
     4921 agacgctcca tttcctccat ctg
//
LOCUS       DASTKM010000255         9469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1496562, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000255 DASTKM010000000
VERSION     DASTKM010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9469
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496562"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..442)
                     /locus_tag="VFQ10_10415"
     CDS             complement(47..442)
                     /locus_tag="VFQ10_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965796.1"
                     /translation="MTRTVLAAVEDLLFRSKISETASSLGIEAAFPRNPRRLLEAIHE
                     SPPDLLILDLNSARFEPLTLLRSVRSDEATRDVPTVGFLSHVQKDLAVAAREAGCDRV
                     VARSAFTKDLQTGKWLEWLSPAPDRHRGP"
     gene            514..2331
                     /locus_tag="VFQ10_10420"
     CDS             514..2331
                     /locus_tag="VFQ10_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9965797.1"
                     /translation="MKRLKELRAGLRRTGETLSNFTPFLRPRRGGLLLALGVLLLETV
                     TSLAQPWPLALTIDYVLTDNKALPDAVPAPLDDKAILLAGIAFLIVLIFTVTRSVASF
                     RRYLLQKLGQQTVFDMRDALYAKVHALGLDYHGKKRTGDTITRVTSDVKEVRTMLVDS
                     VVEVFSSVMILLGMLVVMLWLDWQLTLLALVTVPFLFLAVSRYRKALVQRMRVVRTRE
                     GAIASVMQEAIIGIRAVKLFAREEDEMGRFRKESKESLRASVESAVIEAKFSTVLGIV
                     GGLGTALVVYFGARQVLAGVLSLGDLTVFVAYLALFLSPLWALSRQVNQIGTSLVSGE
                     RIIDLLNAEPTVKDLPGARPAPAFDGRVTFEGVRFAYEHEAGEVLRGMDFDVEAGSRV
                     ALVGVSGAGKTTVTSLIARLYDPQEGRVLIDREDVKAFTLKSLRDSVTFVPQDPLLFR
                     ATVAENIAYGRPGAGREEIEAIAQLAGAHDFIRELPEGYDTVLSERGESLSGGQRQRI
                     SIARAMLRDTPILILDEPQAGLDAEAASAVEESWRALTEGRTTFVIAHELRLVRSVDS
                     ILVIEDGSVAETGTHEDLVSSNGLYARLYSLQERDPVQP"
     gene            complement(2449..3483)
                     /locus_tag="VFQ10_10425"
     CDS             complement(2449..3483)
                     /locus_tag="VFQ10_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET9965798.1"
                     /translation="MTTVVIASFFEEEYVRRIREVDERLRVLYREDLVPPPRWPGDHA
                     GPEGWERKPEDEEEFLAMLGEAEILYDFPGGHVEDLVEVAPGLRWVQGSMAGAGEVAQ
                     KAGLGESDIVVTTASGIYSGPLAEFVLMAMLQHAKELDNLRGDKARKVWRQGAVGTLE
                     NKTLCVVGMGSIGRAIAGRARPFGMRIVGVKRTVREDDEAWDYVDELYPTAELRTALG
                     EADYVAVTLPGTPETRRLLDAEAIAAIKQGAYFANVGRGSVVDEAALVDALQSGHLSG
                     AALDVFEVEPLPEESPLWELDNVIVSAHTTDVVPALINTAQTDLFCENLRRYLAGEEL
                     LNVLDKRLLY"
     gene            3639..4265
                     /locus_tag="VFQ10_10430"
     CDS             3639..4265
                     /locus_tag="VFQ10_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9965799.1"
                     /translation="MDELLDILDESGLPTGQVVPKSEAHRLGLWHRCFHCWICGSDPG
                     GAYLLLQRRAATKDTWPGYLDVTAAGHLAAGEKTLDGLREVEEELGLRIRPERLVPLG
                     TRRVEQEIPGGCDRELHEVFLVFDVTPPGDLRLQKEEVEAVFRLDLDDVEALYENGSA
                     PAREYAEGRTSATRIHLAEFVPKVEGYLRRVAAAARRHLSGAPSVPIF"
     gene            complement(4383..4811)
                     /locus_tag="VFQ10_10435"
     CDS             complement(4383..4811)
                     /locus_tag="VFQ10_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965800.1"
                     /translation="MPARKRTPADAGALAAGLLVDACGPQAENSLRLEVVKDLALDLG
                     RRLEVLAGEDFAADSLVEAALACADLAILAACNLPALPDGDRPLAAAAAHLAAGATRA
                     LIPLVESEAGILDEAYAENMLRDARSAGWRADLAVRQLVG"
     gene            4906..6630
                     /locus_tag="VFQ10_10440"
     CDS             4906..6630
                     /locus_tag="VFQ10_10440"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET9965801.1"
                     /translation="MRVALAQINTTVGDIWGNVEKMADTLERATDSGAELVAYPELTI
                     PGYPPEDLLMRQSFIEENMRALHEFAGRVPENVVAAAGFVDLGTDLYNACALVSGGEV
                     LYRYHKRYLPNYGVFDENRYFREGTGASILDLGDARVGVSVCEDIWYPGGPAREEALG
                     GASVLLNISASPYHRRKGAARERMLGVRAADYGCYVLFCNLTGGQDELVFDGHSVVFD
                     PEGRLVARAGQFREDLLLVDLYPDYSLMQRLHDPRPRKESAEHKPEIVRVPRHETAPA
                     EPVEPRIEPSLPEEGEVLEALVLGLGDYFRKNGFSRAVLGLSGGIDSSLAAAVAVEAL
                     GPQNLTGVLMPSRYTSEGSNTDAAALAKNLGIDTQTIPIGPAFDAYREMLESAFKGLP
                     EDVTEENLQSRIRGNILMALSNKFGWIVLSTGNKSEMSVGYSTLYGDSAGGFAVIKDV
                     PKNLVYRVARHFNERKDREVIPDSILTKEPTAELRENQRDTDSLPPYDVLDPILEAYI
                     EEDRGVAEIVASGFDEEDVRRIVQLVDRAEYKRRQAPTGIKVTGRAFGRDRRMPITNR
                     YFERNHKE"
     gene            complement(6763..7950)
                     /locus_tag="VFQ10_10445"
     CDS             complement(6763..7950)
                     /locus_tag="VFQ10_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9965802.1"
                     /translation="MLSVGTLAWYFVLGPAAAGPGSVREAVAALSQPVCDAALLFLGL
                     VVASSPIRPRFSTLLNGGFVALVLADAAYLGLRSVGPYQSGNWPEMLWALGMILLGLT
                     STTSPEVPADPNVRRIQPWQIILYWLGPLSPPVHFVILLLWGAMNPPLPAYVLAGCAV
                     LLLYMALRIGLVSSVSKHLGQEREESVRAKEQGRLLYELHDTSKQEVHGISLTLRAAL
                     DTERRGDREEALEMFGRALAASREAEFRISRPYVRAFCPTRRASARGRRLPPPAPAEV
                     RGILRGEDPRRHSGPLDVLNPAETAALIRVFVEAFWNVAKHSGASNLYLESRRVGSVL
                     IIRVRDDGHGFDTGKPPPGLGLEYMRRRAGEVGAELDVISTPGRGTSVQVRFRKHQNT
                     ANP"
     gene            8516..>9469
                     /locus_tag="VFQ10_10450"
     CDS             8516..>9469
                     /locus_tag="VFQ10_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9965803.1"
                     /translation="MEYARLGNTGLMVSELCLGCMTFGRETDEATSREIVGRFLDAGG
                     NFIDTADVYSKGISEEITGRAIKGVRDDVVLATKVFFPMGEGPNDVGLSRKHVTQGCE
                     DSLRRLGTDYVDLYQVHCWDGATPLKETLSALTDLVWSGKVRYIGVSNFTGWQLMKSV
                     CMSEANDFERFVCLQPQYSLVERNIEREILPICLEEGLGVIPWSPLGGGFLSGKYRRD
                     KELPEGSRISEAVESMEEHWDRRATERNWATLDVVGRISEQTGRSYAQISLNWLIRQE
                     AVTAPIIGARTLEQLEDNIGASGWKLSGEQVEELSKASSLDD"
BASE COUNT         1779 a         2977 c         3029 g         1684 t
ORIGIN      
        1 tcaatggtag agaggcaaga gttctataga aaaatgaacg tgtcggtcag ggtccacgat
       61 gacgatctgg agctggagat agccactcca gccatttccc agtctgaaga tccttcgtaa
      121 aagcgctgcg ggcgacgacg cggtcgcatc cggcctcccg tgcggcgaca gccaggtctt
      181 tctggacgtg ggagaggaag ccgaccgtag gaacatcgcg tgtcgcttcg tcggacctca
      241 cgctcctcag gagcgttagc ggttcgaagc gcgcggagtt caggtcgagg atgaggagat
      301 cgggcgggga ctcgtgaatg gcttcgagca atctcctggg gttgcggggg aatgccgcct
      361 cgataccgag gctcgatgcg gtctcggaga tcttgctcct gaacaagagg tcttctacgg
      421 ccgcgaggac tgttctcgtc actttcgcct ccgtgctcta gagttgggta agctcgcaca
      481 ttctacccgc gggcggtgcg gtatcctatt cgagtgaaaa gattgaagga acttcgcgcc
      541 gggctaaggc gcacagggga gacgctttcc aactttacgc ccttcttgag gcctcgccgc
      601 ggcgggcttc tgctggcgct tggcgtgttg ctgctggaga cggtgacgag ccttgcgcag
      661 ccgtggccgc tagcgctgac catcgactac gtgttgaccg acaacaaggc gctccctgac
      721 gcggtgcccg ctcctctaga tgataaagcc atacttctcg ccggtatagc attcctcatc
      781 gttttgatct tcacggtaac gaggagcgtc gcctcattcc ggcgctacct gctccagaaa
      841 ctcgggcagc agaccgtctt cgacatgcgc gatgcccttt acgcgaaggt ccacgccttg
      901 gggctcgact accacggtaa gaagcgtacg ggcgacacca taacccgcgt caccagcgac
      961 gtgaaagagg tgcgcaccat gctggtcgac tctgtggtcg aggtgttctc ctcggtcatg
     1021 atcctgctcg gtatgttggt cgtcatgctc tggctcgact ggcagctcac cctcctcgcc
     1081 ctcgttaccg tacctttcct ctttctcgcc gtcagccgct acaggaaggc gctcgtacag
     1141 cggatgcgcg tcgtgaggac gagggagggc gccatcgcca gcgtcatgca ggaggccata
     1201 atcggtataa gggccgtcaa actcttcgcc cgcgaggagg acgagatggg gcgcttcagg
     1261 aaggagagca aggagtccct cagggccagc gtggagagcg cggtgataga ggccaagttc
     1321 agcaccgtac tcgggatcgt cggggggctt ggcacggcgc tggttgttta cttcggggcg
     1381 aggcaggttc tggccggtgt cctctctttg ggtgacctca ccgtctttgt ggcctacctc
     1441 gctctgttcc tctcgccctt gtgggccttg agccggcagg tcaaccagat cggcacatct
     1501 ctcgtctcgg gcgagcgaat catagatctc ctgaacgcgg agcctaccgt gaaggacctt
     1561 cccggagccc gtcccgcgcc tgccttcgac ggccgagtta ccttcgaggg cgtgcgcttc
     1621 gcctacgagc acgaggcggg agaggtcttg cgcggcatgg acttcgacgt cgaagcgggg
     1681 agccgggtcg cgctcgtcgg ggtgagcggc gcgggcaaga ccacggtcac cagcctcatc
     1741 gcccgtctct acgacccgca ggagggaagg gtcctcatcg acagggaaga cgtaaaggcc
     1801 ttcaccctca agtccttgcg cgactccgta accttcgtgc cgcaagatcc cctgctcttc
     1861 agggcgacgg ttgccgagaa catagcctac gggcggccag gggcgggccg agaggagatc
     1921 gaggccatag cccagctagc gggggcccac gattttatcc gggagttgcc cgagggctac
     1981 gacaccgtgc tctccgagcg cggcgagagt ctgagcggtg gacagaggca gcgcatatcc
     2041 atcgcgcggg ccatgctcag ggacactccc atcctgatcc tggacgagcc gcaggccggc
     2101 ctcgacgccg aggccgcgtc cgccgtcgag gagagctgga gggccctgac agaaggacgt
     2161 accaccttcg tcatcgccca cgagctcagg ctggtcagga gcgtagattc gatcctcgtg
     2221 atcgaagacg gcagcgtcgc cgagacgggc acccatgaag accttgtctc ctcaaacggc
     2281 ctctatgcga ggctctactc tctgcaagag cgcgaccccg tgcaaccgta gagggggtga
     2341 aacccttgag agagctgctg atcctcagcg tggtcctgac agccgtggcg gtcgcgatct
     2401 acatctcgtt ctcgtagcgg ccgagtgtta gaccccggag gcagtgccct agtagaggag
     2461 acgtttgtcc agcacgttca gcagctcctc gccggcgagg taacggcgca ggttttcgca
     2521 gaacaggtcg gtttgtgcgg tgtttatcag ggccggcacg acgtccgttg tgtgggcgct
     2581 gacgatcacg ttgtcgagct cccatagggg actctcctca ggcagaggct cgacctcgaa
     2641 cacgtcgagc gccgcccctg agagatggcc gctctgaagg gcgtcgacga gcgccgcctc
     2701 gtcgaccacg gacccccttc ccacgttggc gaagtacgcc ccctgcttga tcgcggctat
     2761 cgcctcagcg tcgagcaagc gcctcgtctc aggggtgccc ggaagcgtga cggcgacgta
     2821 gtcagcctcg ccgagtgcgg tgcgtaactc cgctgtcggg tacagttcgt cgacgtagtc
     2881 ccaggcttcg tcgtcctcgc gcaccgtacg cttgaccccg acgatgcgca taccgaaagg
     2941 tcgagcccgc cctgctatgg cgcgaccgat actccccatc ccgaccacgc acagcgtctt
     3001 gttctcgagc gtccccaccg ccccctgacg ccagaccttc cttgccttgt cgccgcggag
     3061 gttgtcgagt tccttggcgt gctggagcat agccatgagc acgaactcgg ctaaaggccc
     3121 agagtagatg ccgctggccg tcgtcaccac gatatctgac tccccaagac cagccttctg
     3181 tgccacctct cccgcccccg ccatacttcc ctgcacccac cggagtccgg gtgcgacctc
     3241 taccaggtcc tcgacgtgac cgccggggaa gtcgtagagg atctcggcct cccccagcat
     3301 cgccagaaac tcctcctcgt cctcgggctt tctctcccac ccttccggtc cagcgtggtc
     3361 cccgggccac cgtggaggcg ggaccaagtc ctccctgtag agtaccctca accgctcgtc
     3421 tacctccctt atgcgacgga cgtactcttc ctcgaagaaa ctggctatga cgaccgtggt
     3481 caaagagtgc ctccttcgtc ggcagcgatt cagcacgcgc cctgtcgggc gctttcagca
     3541 tttcagcatt tcagcctccg tgaacgcaac tcccggctca ctgctgacaa gccgccgtag
     3601 acggcgtgct gacaagctga catgctactc ttttccgtgt ggatgagctg ctggatattc
     3661 tggacgagtc agggttgcct acggggcagg tcgttccgaa gagcgaggcg caccggctgg
     3721 gtctgtggca ccgttgtttt cactgttgga tctgcggttc cgaccccgga ggggcctacc
     3781 tgctcctcca aaggcgcgcc gctacgaaag atacctggcc tggttacctc gacgtcaccg
     3841 ccgcggggca tcttgcggca ggggagaaga cgctggacgg tttgcgggag gtcgaggagg
     3901 agctcggcct gcggataagg cctgagaggc tcgtcccgct cggaacccgc agggtggagc
     3961 aggagatacc tggcggctgc gacagggagc tgcacgaggt cttcctcgtg ttcgacgtca
     4021 cgcctccggg ggacctgcgt cttcagaaag aggaggtcga ggctgtgttt cggcttgatc
     4081 tcgacgacgt ggaagcactc tacgagaacg gttctgcgcc agcccgcgag tatgctgaag
     4141 gcagaacctc tgcaacgcgg atacacctcg cggagttcgt cccgaaagta gaaggctacc
     4201 tgcgcagggt agccgcagca gcccgtcgac acctctcagg agcaccgtcc gtccctatct
     4261 tctagagcca ttcgcacagg catctaccac gaatcacgca ttcggctaca ggcgatcagc
     4321 gaaaacaagt agctgactaa ctgacgtgct gaaagcgaag cccgaagggc gtagcgtgat
     4381 gactagccga ccagctgcct cacggccaga tcggcccgcc agcctgcgct ccgcgcgtcc
     4441 cgcagcatgt tctcggcgta ggcctcatcc aggattcctg cctctgattc gacgaggggg
     4501 atgagggcgc gcgtggcccc ggccgccagg tgcgcggccg ccgctgcgag aggcctgtcg
     4561 ccgtcgggga gagcgggaag gttgcaggcg gcgaggatgg ccaggtcggc gcatgccagc
     4621 gcggcctcca cgagggagtc ggcggcgaag tcttctccgg cgaggacctc gagccggcgt
     4681 ccgaggtcaa gagccaggtc ctttaccacc tcgagccgca gcgagttttc ggcttgcggc
     4741 ccgcaggcgt ctacgagaag acctgcggcc agggctcctg catccgcggg ggtgcgcttt
     4801 ctggctggca tcgcgtggga ttctatcgga aggttggccg gggcgaagct ctacaggcat
     4861 gagcatcggc cggcacgaac cctccaggca cgatatatta agtgcatgag ggttgcgctg
     4921 gcccagatca acacgaccgt cggagacatc tgggggaacg tcgagaagat ggccgacacc
     4981 ctggagcggg cgaccgattc gggtgctgag ctcgtcgcat accccgagtt gaccatcccc
     5041 ggctatccgc ccgaggatct cctcatgcgc cagagcttta tcgaggagaa catgagagcg
     5101 ctccacgagt tcgccgggcg ggtacccgag aacgtggtgg ccgccgcggg cttcgttgac
     5161 ctgggcaccg acctgtacaa tgcctgcgcc ctggtgtccg gtggcgaggt actgtaccgt
     5221 taccacaagc gttacctacc gaactacggc gtcttcgacg agaaccgcta cttcagggag
     5281 ggtactggcg cctcgatcct cgatctgggc gacgcccgcg tcggggtgag cgtgtgcgag
     5341 gatatctggt accccggtgg cccggcccgc gaggaggccc tcggcggggc tagcgtcctc
     5401 ctcaacatct cggcctcccc ttaccacagg cggaagggcg ccgccaggga acggatgttg
     5461 ggcgtgcggg ctgccgacta cggttgctac gtactcttct gcaacctcac gggcggccag
     5521 gacgagctcg tcttcgacgg ccactcggtc gtcttcgacc ccgagggcag gctcgtggcg
     5581 cgggccggcc agttcagaga agacctgctg ctcgtggacc tgtacccgga ttactcgctc
     5641 atgcaacgcc tccacgaccc caggccgcgc aaggagagcg ccgagcataa gcccgagata
     5701 gtaagggtgc ctcgacacga gacggcccct gcggaaccgg tcgaacctag gatcgagcct
     5761 tcccttccgg aggaaggcga ggtgctcgaa gccctcgtgc taggcctcgg tgattacttc
     5821 cgcaagaacg ggttctcgcg ggccgtgctc ggtctctcgg gcggcataga ttcatccctg
     5881 gccgccgccg tcgctgtcga ggctctcggt ccccagaacc tcacgggcgt cctcatgccg
     5941 agccgttaca cctcggaagg gagcaacacc gacgccgcag cgctcgccaa gaacctcggc
     6001 atagataccc agaccatccc catcgggccg gctttcgacg cttacaggga gatgctggag
     6061 agtgccttca aagggctacc cgaggacgtg acggaggaga acctgcagag ccgcatcagg
     6121 ggtaacatcc tgatggccct ctccaacaag ttcggttgga tcgtcctgtc cacgggcaac
     6181 aagagcgaga tgagcgtggg ctactccacc ctctacggcg actcagcggg cggtttcgcg
     6241 gtcataaagg acgtgccgaa gaacctggtc tacagggtgg cgcggcactt caacgagagg
     6301 aaggatcgcg aggtcatccc cgactcgatc cttaccaagg agccaaccgc cgagctgcgc
     6361 gagaaccagc gagacacgga ctccctgccg ccctacgacg ttctagatcc catcctcgaa
     6421 gcctacatcg aggaggacag gggtgtggct gagatcgtgg cctcaggttt cgacgaggaa
     6481 gatgtgcgtc gcatcgtgca acttgtggac agggccgagt acaagcgccg tcaggcccct
     6541 acgggcataa aggtcacggg cagggccttc ggccgcgaca gacgtatgcc catcaccaac
     6601 cgctacttcg aacgcaacca caaagaatag ctgttaatcc tcgccccgcc tgacagatat
     6661 atgcaggtcc tgaacggcgg tgatggaccc cgtcacgagg aggctccttt cgtttgccgg
     6721 ctacattctg ctcggcctcg tggagcctgt ctaatcgccc ggctaggggt tggcggtgtt
     6781 ctggtgcttg cggaacctta cctgcaccga agtaccgcgt ccaggagtgg agatcacgtc
     6841 cagttccgct ccgacctccc cggcgcgccg gcgcatgtac tctagaccga gacctggcgg
     6901 cggttttccc gtgtcgaagc cgtggccgtc gtcccgaaca cggatgatga gcactgagcc
     6961 tacccgacga gactccaggt acaggttgct cgcccccgag tgcttggcga cgttccagaa
     7021 tgcctcgacg aacaccctga taagggctgc ggtctcggcc gggttcaata cgtcgagggg
     7081 gcctgagtgt cgtcgtgggt cttcaccccg aagtattcct cgaacttccg caggcgctgg
     7141 cggaggaagt ctgcggcccc tggcggacgc tcgccgcgta gggcagaaag ctcgtacgta
     7201 ggggcgtgag atcctgaact cggcctccct cgacgctgcc agggctctcc cgaacatttc
     7261 cagcgcctcc tcacggtctc cgcgacgctc ggtgtcgagg gcggcccgca gggtcagcga
     7321 gatgccgtgc acttcctgct ttgacgtgtc gtgtagctcg tagagaaggc ggccctgctc
     7381 ctttgcacgg accgattcct cccgctcctg gccaaggtgt ttgctgaccg acgagaccag
     7441 cccgatccgt agtgccatat acaggaggag cacggcgcat cccgcaagca cgtaagccgg
     7501 caacggtgga ttcatcgcgc cccagagtag caggatcacg aagtgtaccg gcggggagag
     7561 cgggccgagc cagtacaaga ttatctgcca cggctgtatc cgcctgacgt tcgggtcggc
     7621 aggaacctcg ggtgatgtcg tcgaagtgag gccgagcagg atcatgccta gagcccacaa
     7681 catctccggc cagttgccgc tctggtacgg cccgacggag cgaagtccca ggtaagcggc
     7741 atcggcaagg acaagcgcca cgaaaccccc gttcagaagc gtggagaaac ggggtctgat
     7801 cggggacgag gcgaccacga gcccgaggaa gagcagggcc gcgtcgcaga caggctggga
     7861 gagggccgcc acggcctcgc gtacactccc tggcccggcc gcagcgggac cgaggacgaa
     7921 gtaccaggcc agcgtcccga ccgagagcat gacggccccc gcgtcgaggg cgctaccagc
     7981 gttatcctgc gagaggtcag cgccacgagg tgaagcatcg cccctaccag caacgagtat
     8041 gagatggcgt aggcgacgtc ttgcggggcc accggcgtga cgatgccaag ccccaagtgt
     8101 tgcgacgcgc tccagagtga gttaccggcg aacctgaaga ccatgcccag tcccatgagc
     8161 cgcaggaagg cccgctcgtg gccctctgcg cgcggcgcgg cccgcagcag gatcacggcg
     8221 gcaaaggcgg ccgtagatag ggagaagacc gtggcgatta cggatttcga ctccgcgccc
     8281 tggggcagaa acgcgaagac gagcgcgccc agagcccagc aggccagaac ggctgtcgta
     8341 gggatgcgcg ggacgcgagg atcgcgcaac ctacccgcat ccgaaccttg caaccctcca
     8401 gaactcgaac ccaccaatcc taccaccctc gtgccgtgcg tggccaaccc agagggccaa
     8461 acctcgtatg ttaaagtacg gcctttcggc ggcagagatc cgagaggagc agcgtatgga
     8521 gtacgcacgt cttgggaaca cggggctgat ggtctccgag ctgtgcctgg ggtgcatgac
     8581 cttcggacgg gagacggatg aggcgacctc tagagagatc gtcggcaggt ttctcgatgc
     8641 aggaggcaac tttatagaca ccgccgacgt ctactcgaag ggtatctcag aggaaatcac
     8701 gggtagggcg ataaaggggg tccgtgacga cgtggtgctc gccacgaagg tcttctttcc
     8761 aatgggagaa ggcccgaacg atgtcggcct ctcgcgcaag cacgtgacgc agggttgcga
     8821 ggacagcctc aggcgtctcg gcacggacta cgtcgacctc tatcaagtgc attgttggga
     8881 cggcgccact cctttgaaag aaactctctc ggcactcact gatctcgtct ggagcgggaa
     8941 ggtgcgctat ataggcgtct ccaactttac gggctggcaa ctcatgaaga gcgtgtgcat
     9001 gagcgaagcg aacgacttcg agaggttcgt ctgcctgcag ccgcagtact cgctcgtcga
     9061 gcgcaatatc gagcgcgaga tccttccgat ctgccttgaa gagggactcg gggtgatccc
     9121 ttggagccca cttggcggtg gcttcctctc aggaaagtac aggcgcgaca aggagctgcc
     9181 cgagggctcg cgcatctccg aagcggtgga gtcgatggaa gagcactggg accgccgcgc
     9241 cacagagcgc aactgggcca cgctggacgt ggtaggcagg atctccgagc agactggcag
     9301 atcttacgcc cagatctcac tgaactggct catcaggcag gaggccgtta ccgcccctat
     9361 catcggcgcc cgcacgctgg aacaactgga agacaacatc ggcgcgagtg gttggaagtt
     9421 gtccggggag caggtggaag aactctccaa ggcaagctcc ctcgacgac
//
LOCUS       DASTKM010000256         4001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1146671, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000256 DASTKM010000000
VERSION     DASTKM010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146671"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..423)
                     /locus_tag="VFQ10_10455"
     CDS             complement(16..423)
                     /locus_tag="VFQ10_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965804.1"
                     /translation="MKIHGKIPRLFREPPRTLFDDPRYEAFEAPVKGPLFSNSVFYTL
                     LAVFGTFLMLTADWFGLLLLNSEGVFQRIGPLNTVRVTAVLMTGIAIPSALLMFHYKR
                     LYSYTSDENVFFAVTFSLSFCLGSPCVLTGIFG"
     gene            complement(464..1300)
                     /locus_tag="VFQ10_10460"
     CDS             complement(464..1300)
                     /locus_tag="VFQ10_10460"
                     /inference="COORDINATES: protein motif:HMM:NF009499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9965805.1"
                     /translation="MSDTRSTVSTNQARSSAVPASGDLRGRVALVTGVGRRRGIGSAV
                     CLALASRGAGVVLSYWKAYDREMPWGSDEDEPEALLGELRAAGVRAEGVEADLSRPDS
                     PESLLDAAGERLGRPSILVNTAAYSTHDGFETLDAETLDAHYAVNVRAMALLSVGFAR
                     RYPGGPGGRIVNFSSGQSLGPMPGELAYVATKGAIEAFTRTLAAEVGHKGITVNAINP
                     GPTDTGWMTEELKREIAPRFPSGRIGQPEDAARVVAFLVGDEAAWITGQVIHSEGGFF
                     RS"
     gene            complement(1426..2580)
                     /locus_tag="VFQ10_10465"
     CDS             complement(1426..2580)
                     /locus_tag="VFQ10_10465"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HET9965806.1"
                     /translation="MNLSVLTRVVPDGGFLAVRRRFEEAFGRLGVGVWPILQTAVAAS
                     LAWFLASAALGHDQPFFAAIAAVISTGVVVGQEGRRSVELVFGVACGLAVADLLVLAI
                     GTGMIQIGIVVALAMAAAMMLGGGTLLITEAGVSALLAITLEPSTTGLSPERFVDALV
                     GSGVALAVRAVFPSDPRHIVERAAHPIFDDLVGALEETAAALRECDLGRAERALQKAR
                     EMDGRMGAFREALDAGYDTVRLSPPRRRALGQLGLYATAADQLDLAVRNVRVLARAAA
                     AAVREGEPAPEALSETIRDLARAVETLSAYIEEPEHPMETRRFALEAAVGATAVLLER
                     NDLRTSMLVGQIRSTAVDLLRASGMDSTESLEALRKAANRTTQEPTDQAV"
     gene            complement(2908..3771)
                     /locus_tag="VFQ10_10470"
     CDS             complement(2908..3771)
                     /locus_tag="VFQ10_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET9965807.1"
                     /translation="MNLTFYIVDVFAEEKYAGNQLAVVRGGAGLPDETLQKIALEMNY
                     SEPTFVLSEEETGGGYDVRIFTPGDEIPFAGHPTLGTAYVIQYEILAGPVESITLKLK
                     AGEIPVTFGEVLWMRQLPPTFGATLDYALIAPTLNLEMADLDDRSPVQEVSTGLPALI
                     VPLRDLDALRRCKVDWERHTEVAGPGKNLYVFCPESHDDGPGDLSARMFANDPGVPED
                     PATGSAAGCLAAYLLEHGYLGTDSIDVRVEQGYEICRPSLLYLQAARDGDQTTVHVGG
                     KVQMVARGELV"
     gene            3826..>4001
                     /locus_tag="VFQ10_10475"
     CDS             3826..>4001
                     /locus_tag="VFQ10_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9965808.1"
                     /translation="MATRKPYPSDVCDEEWAFVAPYLALVREDAPQRNHDLREVFNGL
                     RWIVRTGSAWRYMP"
BASE COUNT          757 a         1274 c         1259 g          711 t
ORIGIN      
        1 atacctgtaa tctactcatc cgaatattcc cgtgaggaca cagggggagc ccaggcagaa
       61 agagagcgag aaggtgacgg cgaagaagac gttctcgtca gaggtatagg agtagaggcg
      121 cttgtagtgg aacatcagga gcgccgatgg gatggctatc ccggtcatca agacggccgt
      181 cacgcgcacc gtgtttagag gaccgatcct ctggaagacg ccttctgagt tcaagagcag
      241 cagcccgaac cagtcggcgg tcagcatgag gaaggtgccg aagacggcca ggagcgtgta
      301 gaacacggag ttcgaaaaca gcggcccctt gacgggcgcc tcgaaggctt cgtagcgcgg
      361 gtcatcgaac agcgtcctcg gcggttcgcg gaagagcctg gggatctttc cgtgaatctt
      421 catgcgagat gtaatggtag cgcaaggaag tctaaaatgc cgctcaagaa cggaagaagc
      481 cgccctcgga atgaatgacc tggcccgtga tccaggccgc ctcatcaccg accaggaacg
      541 ccacgaccct cgccgcgtcc tcgggctgcc cgatcctccc cgaagggaac ctcggtgcaa
      601 tctcccgctt caactcctcg gtcatccagc ccgtgtccgt agggccaggg ttgatggcgt
      661 tcaccgtgat gcccttgtgc ccaacctccg cagcgagggt cctggtgaac gcctcgatgg
      721 cgcctttggt cgcgacgtag gcgagctctc ctggcatcgg ccctagcgac tgtcctgaac
      781 tgaagttgac gatcctgcca ccaggcccac cgggataccg ccttgcgaag ccgacgctga
      841 gtagcgccat ggcccgcacg ttgacggcgt agtgggcgtc cagcgtctct gcgtcgaggg
      901 tctcgaagcc gtcatgggtg gagtaagcgg cggtgtttac gaggatcgag ggtctcccga
      961 gcctctcccc agccgcatct agcagagact cgggggaatc aggtctggag aggtcggcct
     1021 ctaccccctc tgcccgaacg ccggcggcac gcagctcccc tagcagcgcc tcaggctcgt
     1081 cttcgtccga cccccagggc atctcgcggt cgtaagcttt ccagtaagag aggacgacgc
     1141 cggcgccgcg ggaggcgagc gccaggcaga ccgcggagcc tatccccctc ctcctcccga
     1201 caccggtcac gagcgccacg cggccccgca aatctccgct ggccggaact gcggaagagc
     1261 gggcctggtt ggtggaaacc gtggatctgg tgtcggacat gggcctctcc tcgacatagc
     1321 gccggccggg aatgacggat gaagagaaag ctagcatgcc cccaagcgcg cctcaagccg
     1381 cctcgaagca gtggtgccgc gccggacctg gagcatctcc ccggctcaga cagcctggtc
     1441 cgtgggctcc tgggttgtac ggttggcggc tttgcggagc gcttcgagag actcggtcga
     1501 atccatgccc gaggcccgca ggagatccac ggcggttgac ctgatctggc ccaccagcat
     1561 gctggtcctg aggtcgttgc gctccaagag tactgcggtc gcccccaccg cggcttcgag
     1621 ggcgaacctg cgggtctcca taggatgttc cggttcttca atgtaggctg agagggtttc
     1681 gacggcacgg gcgaggtcgc ggatggtctc ggagagcgcc tctggggcgg gctctccctc
     1741 gcgtaccgcc gccgcagcag cccgcgcgag taccctgacg ttgcggaccg cgaggtccaa
     1801 ctggtcggcc gcagtagcgt agagcccgag ctgtccgagc gcccgcctgc gcggcgggga
     1861 gagcctgacg gtatcatagc cggcgtcgag cgcctccctg aaagcgccca tacgtccatc
     1921 catctcacgt gccttctgca aggcgcgctc tgctcggccg aggtcacact cccgcagggc
     1981 cgctgccgtc tcttcgaggg cgccgacgag gtcgtcgaag atcgggtgcg cggcccgttc
     2041 tacgatgtgc cttgggtcgc tggggaacac cgccctgacc gcgagggcca ccccgctccc
     2101 gaccaacgcg tcgacaaacc gctcaggcga aagacccgtg gtggaaggct cgagcgtgat
     2161 cgccaacagc gccgataccc cagcctcggt gatgagcaac gtcccaccac cgagcatcat
     2221 ggcggcagcc atagccaggg ccacgacgat cccgatctgg atcatccccg tcccgatggc
     2281 gagcacgagg aggtcggcga ccgcaagtcc gcacgccacc ccgaacacca gctcgaccga
     2341 acgccgcccc tcctggccga cgacgacgcc ggtggagatg acagcggcta tagcggcgaa
     2401 gaagggctgg tcgtgcccga gcgcagcaga agccaaaaac cacgccagac tcgccgccac
     2461 cgccgtctgc aagataggcc ataccccgac ccccaaccgg ccgaacgcct cttcgaaccg
     2521 gcgtcgtacc gccaagaacc cgccatcagg cacaacccgc gtaagcacgc tcagattcac
     2581 aaccaaacac ctcctttaga ttcactcaat acctatatgc gctctgcata caagcgacag
     2641 taacacgaca accctggctg ttaaagggat tcgggggtgt ttaagcaatt tgttattatg
     2701 ctgttacttg gttgttactt tctccagatt cgacccttgc cgaggagggc gagtctaccg
     2761 agtgagcctg gctgacgaac tccttccgca cgccctcggg tatgctctcc ctgtaggtat
     2821 gatcccacgt tgtccgtgcg acattgtgga tctcctgaac gtcaacgacg ccagcggctc
     2881 ggatctcgac tcgcctggag tcccggctca caccaactct cccctcgcca ccatctgtac
     2941 tttgccgccg acgtggacgg tcgtctggtc tccatctctc gcggcctgca ggtacaggag
     3001 cgaggggcgg cagatctcgt acccctgctc gactcgcacg tctatggagt cggttccgag
     3061 gtagccgtgt tcgagtaggt aagcggccag gcagcctgct gcgctgccgg tcgccgggtc
     3121 ttcggggaca ccggggtcgt ttgcgaacat gcgggccgag aggtcgcctg gaccgtcgtc
     3181 gtgcgattcc gggcagaaga catagaggtt ctttccaggg ccggccactt ccgtatggcg
     3241 ttcccaatct actttgcagc gccgcagagc gtccaggtct ctgagcggga ctatgagggc
     3301 cggaagtccg gtggatactt cttgcacggg agagcgatcg tcgaggtccg ccatttcgag
     3361 attcagggtt ggggcgatca gggcatagtc gagggtcgct ccgaaggtag gcgggagctg
     3421 gcgcatccag agaacttcgc cgaaggtgac ggggatctca ccggccttca acttcagggt
     3481 gatactctcg actggtccgg cgagtatctc gtactggatc acgtacgcag taccgagggt
     3541 ggggtggccg gcgaagggta tctcatcgcc cggcgtgaag atgcggacgt cgtagccacc
     3601 tccggtctct tcctccgaca ggacgaaggt gggctcggag tagttcatct ccagggctat
     3661 cttctgaagc gtctcatctg gcaagccggc gccgccgcga acgacggcga gctggttgcc
     3721 cgcgtatttc tcttcggcga agacgtccac gatgtagaaa gtgaggttca cggtcgctcc
     3781 tagagggtgt tatgaacttc aacctggaag cggtagcatc ttggcatggc gactcgaaaa
     3841 ccatacccaa gtgacgtttg cgacgaagag tgggcgtttg ttgccccgta cctggcgctc
     3901 gttcgagaag acgccccaca gcgcaaccac gacttgcgcg aggtgttcaa tggactgagg
     3961 tggatcgtgc gcacaggctc cgcctggcgc tatatgccac a
//
LOCUS       DASTKM010000257         7282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1497252, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000257 DASTKM010000000
VERSION     DASTKM010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497252"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="VFQ10_10480"
     CDS             complement(<1..1471)
                     /locus_tag="VFQ10_10480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9965809.1"
                     /translation="MGRLGDRFVLVRELGSGGMSRVFLGRDEVLDRPVAVKIVEADPE
                     DPEIGLRFQREGRSAARLSHPNIVRVFDAGEDELDGRGVSYIVMEYVPGGDFEDLIDR
                     NGPIPETMLLRIGADGASGLTHAHERGIIHRDIKPRNILLDERGSPKLADFGIARALD
                     GTYLGTVAYASPEQLRSQRVTPKSDVYSFGATLYHAAVGEQPFSGNSIEVANQHVLKV
                     PAPPRERGARISERLETLILSCLAKKPSERPDAARLHDGLLQNGAAEATAALAAPAAR
                     ARDGGLPGAAGTSRLTGAARSAGPSGVGAVRRRMAHKSPSSSPPESTISLPTRTFRAG
                     SRQRMMLAALLAALLLLLLTVAGIWALLGQEEQGGGTPGQGAGGNEQAGGGGPQKSKE
                     ELGKGAGEAVRNPSGGSGSSDGTQGENPAPAPPLVRAERVVYNLYYQESFNRVDASWA
                     YLSERLQNEIGSPERWAEREDIYTFTYMEFTSYPVARAVG"
     gene            complement(1498..2310)
                     /gene="aroF"
                     /locus_tag="VFQ10_10485"
     CDS             complement(1498..2310)
                     /gene="aroF"
                     /locus_tag="VFQ10_10485"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET9965810.1"
                     /translation="MDVSAGVKTNVKTTNGMIRPDTFRVIAGPCTVESYEQFVASAKA
                     VRQAGFDYVRGGAFKPRTSPYSFQGLGEEGLKILSEVSGDLGLKVVTEVMDPYDIEMV
                     MEHAQILQIGARNMANFSLLRRVGGAIAGTDHGVVLKRGLSATVEEWLLAAEYVTAAG
                     GDNVVLCERGIRTFETSTRFTLDLSAVIVAKRLTDLPVIVDPSHAAGRRDLVVPLSKA
                     AVAAEADGLMVESHHEPQEALCDGEQALPVEALMGMKDVLQPFASAMGREVI"
     gene            complement(2312..2593)
                     /locus_tag="VFQ10_10490"
     CDS             complement(2312..2593)
                     /locus_tag="VFQ10_10490"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET9965811.1"
                     /translation="MQERIRELRERVDEVDRELLLALSERARIVQEIIDLKAEAGTPI
                     YDPKREEEILRRVVDRNPGPIYDSSMRDIFELILHRIRDLEIQRGEFPR"
     gene            2837..3898
                     /locus_tag="VFQ10_10495"
     CDS             2837..3898
                     /locus_tag="VFQ10_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9965812.1"
                     /translation="MIFRSELDPLSPYRPPRAANEAAAERGADGYVKLTSNELSFGPL
                     PEAEAALAEALPRANRYPDRYVSSLREEVVSANPGSAFENILVGNGSSEILLNLLQLV
                     ELPGEVIFPWPSFGLYASIGTILGLQTTKVPLTEDHEIKPEALLSSVLDRTRAVIVSN
                     PNNPTGTYLTFQEVRAFAEALPAGVLLILDEAYQEFVSDPAYAGSHVLALEMENVVCV
                     RTFSKAHGLAGFRAGYGIAGQRVADYVERVRFPFSVNLAAQVAASASMRARDKIEARA
                     RFVMGERERIQAAFRGAGLNYIPSQGNFVLVQTGPEIFERAGVLVREGDPLGYPGWSR
                     VTIGDSGENDLVIGALGGV"
     gene            3895..4941
                     /locus_tag="VFQ10_10500"
     CDS             3895..4941
                     /locus_tag="VFQ10_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET9965813.1"
                     /translation="MSGTLGIIGVGLIGGSVGLAARDSGWEVVGVDTPQVLEKAAQSG
                     AIHRASTLKEVREADLVVLAAPISKVTDLVGDLTPTNALVTDVASAKSAIVRRAENLK
                     LRFVGGHPMAGSQLSGVANASSNLFEGARYFLTPTGRTDPEDYREVAGFVRELGAVPT
                     AVDPEKHDLLMAALSHLPHLMAAALLKVASDISPEALSFAGPSFRDLTRVGASNPELW
                     SDILAENAPALGEALGAFAGAMAQLGSEIKDRESMEGRFRQAREAYDALGGILVEKGG
                     ANVDVAIPVENRPGVFAEVTTLMGSNNINILDLYVRHSNTERAALVLTLDAEAAQQAR
                     ELLRMAGFTAELQK"
     gene            complement(4964..5527)
                     /locus_tag="VFQ10_10505"
     CDS             complement(4964..5527)
                     /locus_tag="VFQ10_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965814.1"
                     /translation="MSDRPGEGTSLVDHWQGLKIRMDDRQAVYRRSWLRAPLGEPAHQ
                     PGGKELASDLNLACRLYLQGEETLRREIRGSFEGWTAVRGRMLAKAWSFADKLADTCD
                     DRWLRLGLAAVSIDDNGTDFRDTYVALGALYLCAVRCGMEPGSYFEEAAEISSGVSNM
                     EQTSMRDFLLRFEGSAYFGESVAPKLD"
     gene            complement(5527..6489)
                     /locus_tag="VFQ10_10510"
     CDS             complement(5527..6489)
                     /locus_tag="VFQ10_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HET9965815.1"
                     /translation="MNILVSGATGFIGSALVLDLERLGHRVIRLSRSKPSAEDTIRWD
                     PVSGVLEPSRLGGLDAVVHLAAESVGEGRWTPAKKRRIMDSRREGTRLLAETIAGLPE
                     PPGVMVSVSGINYYGSRGNELLREESGPGNSFLARVCQGWERAADPARRAGVRVVHPR
                     FGIVFPQGFSRDLLGIKVEIEHPKFEITFGTRGSTFARLPVYKLTGGGWIGSGRQFWS
                     LLVAEDVVGVILHALTTDSLQGPVNAVSSSVTAREYTATLNRILNRPTVFPLPAPVAR
                     LALGEFAEEMLLASIRVDSTKLTDSGYEFRYPELAGALRHLVEE"
     gene            6777..7193
                     /locus_tag="VFQ10_10515"
     CDS             6777..7193
                     /locus_tag="VFQ10_10515"
                     /inference="COORDINATES: protein motif:HMM:NF036989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965816.1"
                     /translation="MYSRWGVGMSESEKITINLGPVDLGQIDLLVQEGFYSNRTDFIR
                     TAIRNQLTTHAEEVRQTVARKTLVLGLQNYTRRDLEAVREAGETLEIRVLGLASIDDD
                     VDPELALETIDSLIVLGALRASPAVKAALKGRIQSS"
BASE COUNT         1364 a         2402 c         2192 g         1324 t
ORIGIN      
        1 caccgactgc cctggccacc gggtacgagg tgaactccat gtaggtgaac gtgtagatgt
       61 cttcacgctc ggcccaccgc tccggtgaac ctatctcgtt ctgtagccgc tcggagaggt
      121 acgcccagga ggcgtctacc ctgttgaaag actcctgata gtacaggttg tatacgactc
      181 tctcagccct cacaagcggc ggggctggag ccgggttctc tccctgggta ccgtccgagg
      241 accctgatcc acccgaggga ttgcggaccg cctcaccggc tcccttcccc agctcttctt
      301 tggacttctg cggtccgcca cccccggctt gttcgttccc gccggcgccc tggcccggag
      361 tcccgccacc ctgctcctcc tggcctaaca gcgcccagat gcccgcaacg gttagtagca
      421 aaaggagtag ggcggccagg agagcggcca gcatcatcct ctgccgagag cctgccctga
      481 aggtgcgtgt cgggaggctt atcgtactct cgggtgggct tgaagaagga ctcttatggg
      541 ccatccttcg tctgactgcc cccacaccag aaggcccggc cgacctggct gcccctgtca
      601 agcgcgaggt tcctgccgca ccagggagcc caccgtcccg ggctctggcc gccggcgcgg
      661 caagtgcggc ggtcgcctcg gccgcgccgt tctggagcaa cccgtcgtgg agacgggccg
      721 catcggggcg ctcggaaggc ttcttggcga ggcaactgag gatcagagtc tcgagccgct
      781 cgctgatccg cgctcccctc tccctgggag gtgcgggaac cttcagtacg tgctggtttg
      841 cgacctctat ggagttgccc gagaagggct gttccccgac ggccgcatgg tagagggtgg
      901 caccgaacga gtagacgtca ctcttgggag taaccctctg actcctcagc tgctctgggg
      961 aggcataagc caccgtcccg aggtacgtcc cgtcgagggc ccgtgctatg ccgaagtctg
     1021 caagtttggg actcccccgc tcatcgagca gaatgttgcg cggctttatg tcgcggtgga
     1081 taatgccccg ctcgtgggcg tgggtgaggc ccgaggcgcc gtcggcccca atccgcaaga
     1141 gcatcgtctc gggaataggt ccgttcctgt cgatcagatc ctcgaagtcg ccgccaggga
     1201 cgtactccat gacgatatag gagaccccgc gcccatcgag ctcgtcctcg cccgcgtcga
     1261 agaccctgac gatgttgggg tgggagagcc tggcggcgga cctcccctcg cgctggaatc
     1321 gcagaccgat ctcagggtct tcgggatccg cttccacgat cttgacggcg acgggacggt
     1381 cgagtacctc atcgcgtccc aggaagacac gggacatccc ccctgagccg agctcgcgca
     1441 cgagcacgaa cctgtccccg aggcggccca cagaacgtcc ggggcccatg cccgagacta
     1501 gataacctcc ctgcccatgg cactggcgaa aggttgcaac acgtccttca tacccattag
     1561 ggcctctaca gggagcgcct gctccccatc gcacaacgcc tcctgcggct cgtggtggct
     1621 ctccaccatc aggccgtcgg cctcagccgc taccgctgct ttcgagagcg gcacgaccag
     1681 gtcgcgccgt ccggcggcgt gggaggggtc aacgatcacg ggcagatccg tcagcctctt
     1741 ggccacgatc accgcagaga ggtccagcgt gaaacgcgtg ctcgtctcga aagtgcggat
     1801 tccgcgttcg cacaagacta cgttgtcccc acccgccgcc gtgacgtact cggccgcgag
     1861 cagccactcc tcgaccgtcg ccgagagccc cctcttgagg acgacgccgt gatccgtgcc
     1921 ggctatcgcc ccgccgacgc gcctgagcag ggagaagttg gccatgttgc gggcgccgat
     1981 ctgcaatatc tgcgcgtgct ccatgaccat ctctatgtcg taagggtcca ttacctcggt
     2041 gacgaccttc aggccaaggt ccccggagac ttcggagagt atcttgagac cctcctcgcc
     2101 cagtccctgg aaggagtatg gggaggtgcg gggcttgaaa gcaccgccgc ggacgtagtc
     2161 gaagccggcc tgccttacgg ctttcgcgga ggcgacgaac tgctcgtaac tctcgaccgt
     2221 acatgggccc gctatcaccc tgaaggtgtc cggccggatc atgccgttgg tggtctttac
     2281 attggtcttt acgcctgccg aaacgtccat gttacctggg aaactccccg cgttggatct
     2341 caaggtcgcg gatgcgatgc aggatgagct cgaaaatatc tctcatcgag gagtcgtaga
     2401 tcggacctgg gttgcgatcg accaccctcc gcaggatctc ctcctcacgt ttggggtcgt
     2461 agatgggggt acccgcctct gccttgagat caatgatctc ctgcacgatg cgcgcccgct
     2521 cgctcaacgc caggagtagc tcccggtcaa cctcgtctac tcgctccctc agctccctga
     2581 ttctctcctg caacttggta tccgccatag gatctggaat tgtatcaagt agccagtcag
     2641 catgtcagcc gattggcttc tcagctagaa gcaagatccc atacctgccc gattgcacca
     2701 acgcccgttg aaagctcaaa caaagaagaa tagtctttag tggcatagat tggcgaaaga
     2761 ctgacttgct gaagtgttga caagcggagg ccgacgcgtg ctgaaatgct gagaagccaa
     2821 taggagcgta gcccttttga tattcagatc cgagctagac ccgctatccc cttacaggcc
     2881 gccgcgcgcg gcgaacgagg cggccgcaga gcgaggcgcg gacggttacg tcaagctgac
     2941 ttcgaacgag ctctctttcg gccctctgcc tgaggccgaa gcagccctcg cggaggcgct
     3001 accacgcgcc aaccgctacc ccgaccgcta cgtatcatcc ctgcgcgagg aggtcgtctc
     3061 cgccaacccc ggcagcgcct tcgagaacat actggtcggc aacgggtcga gtgagatcct
     3121 gctcaaccta ctccagctcg tcgagctgcc aggagaggtg atcttcccct ggccatcctt
     3181 cggcctgtac gcctctatcg ggaccatatt gggattacag accacgaagg tccccctcac
     3241 tgaggaccat gagatcaaac cggaagccct gctctcctcc gtgttggata gaacccgcgc
     3301 cgtcatcgtc tccaacccga acaatccgac aggaacgtac ctcaccttcc aggaggtgcg
     3361 cgctttcgcc gaagccctcc ccgctggtgt gctcctgatc ctggatgagg cttaccagga
     3421 gttcgtctcc gacccggcct atgccggctc gcacgttctc gccctcgaaa tggagaacgt
     3481 ggtatgcgtc cgcacgttct ccaaggcgca cggcctcgcc ggcttccgag caggctatgg
     3541 gatagccggg cagagggtgg ccgactacgt cgagcgggtg cgcttcccct tctccgtgaa
     3601 cctggccgcc caggtggccg cctccgcatc gatgcgcgcc agggacaaga tcgaggcccg
     3661 cgcccgcttt gtgatggggg agcgggaaag gatccaggcc gccttcaggg gcgccggcct
     3721 gaactacatc ccctcccagg gcaacttcgt cctggtacag acgggaccgg agatcttcga
     3781 gcgggccggc gtactggtgc gagaaggaga ccctctcggc taccccggtt ggagccgggt
     3841 gacgatcggt gactctggtg agaacgacct ggttatagga gcgctcggag gagtatgagc
     3901 ggcacgctgg gcattatcgg ggtcggcctg atcgggggct ccgtcggcct cgccgccagg
     3961 gactcgggat gggaggtagt cggcgtagac accccgcagg tgctcgagaa agccgcccag
     4021 agcggcgcca tccaccgcgc ctccacgcta aaggaggtac gcgaggctga cctcgttgta
     4081 ctcgcggcgc ctatatccaa ggtgacggat ttggttgggg atctcacccc gaccaacgcc
     4141 cttgtcacgg acgtcgcgag cgcaaagagc gccatcgtgc gcagggcgga gaacctgaaa
     4201 ctacgcttcg tgggcggaca ccccatggcg ggcagccagc tctctggggt cgccaacgca
     4261 agctcgaacc tcttcgaggg agcccgttac ttcctcaccc caacgggccg aaccgatccg
     4321 gaggactacc gtgaggtcgc cgggttcgtc agggaactgg gcgccgtccc taccgccgtc
     4381 gatccggaga agcacgacct cctgatggcg gcgctctcgc acctgccaca cctcatggcg
     4441 gcggccctcc tgaaggtcgc ctcggacatc tcccctgagg cgctctcctt cgccggcccc
     4501 tctttcaggg acctgaccag ggtgggcgct tccaacccgg agctatggtc ggacatcctg
     4561 gcagagaacg cccctgcgct cggtgaagcc ctgggagcat tcgccggggc gatggcgcag
     4621 ctagggagtg agatcaaaga ccgcgagagc atggagggcc gtttcaggca ggcccgcgag
     4681 gcctacgacg ccctcggcgg tatcctagtc gagaagggcg gcgcgaacgt ggacgtggcg
     4741 atccccgtcg agaaccgccc cggcgtcttc gccgaggtaa cgaccctgat gggctcgaac
     4801 aacatcaata tcctcgacct ttatgtgaga cactcgaaca cggaaagggc cgccctggtt
     4861 ctgacactgg acgccgaagc agctcaacaa gccagagagc tactccgcat ggcgggtttc
     4921 accgctgagc ttcagaagta gaaagggcgc cgcctcggtt tactcaatct agcttgggtg
     4981 ctacggattc cccgaagtat gccgatcctt cgaagcggag caggaagtcc cgcatcgagg
     5041 tttgttccat gttcgagacg ccagaggaga tctccgcggc ttcttcgaag taggatccag
     5101 gttccatgcc gcaccggacg gcgcagaggt acagtgcccc gagcgccacg taggtgtccc
     5161 tgaagtcggt gccgttatcg tctatggaga ccgcggctag cccgagacgc agccagcggt
     5221 cgtcacaagt gtcggcgagc ttatcggcga acgaccacgc tttggccagc atacgtcccc
     5281 gaacggccgt ccacccctcg aaggagcctc gtatctcgcg ccgcagggtc tcctcgccct
     5341 gcagatacag tcggcaggcc aggttcaggt cggacgcgag ctcctttcct cccggctggt
     5401 gagcgggctc ccccagcggc gcccgcagcc agcttcgacg gtacaccgcc tgacggtcgt
     5461 ccatccggat cttcaaccct tgccagtgat ctaccaagga cgttccttcg ccaggccggt
     5521 cagacactac tcctccacta gatgtcgcag tgcgcccgca agctccgggt agcgaaactc
     5581 gtagcctgag tctgtgagct tggtggaatc aacacggatg ctggccagga gcatctcttc
     5641 ggcgaattct cccaaggcta gtcgggctac gggagcggga agcgggaaga cggtcggacg
     5701 attcaagatg cgatttaggg tcgcagtgta ttcgcgggcg gtgacggaac tcgacactgc
     5761 gttgacagga ccttgcaagg agtcggtggt aagcgcgtgg agtatcacgc ccaccacatc
     5821 ctcggcgacg agcaagctcc agaactgcct gcccgacccg atccaccctc cccccgtaag
     5881 cttgtagacg ggtaacctgg cgaacgtaga gcctctagtc ccgaacgtga tctcgaactt
     5941 gggatgctcg atctccacct tgatgcccaa gagatcccgg gaaaatccct ggggaaatac
     6001 tattccgaac ctgggatgca cgaccctgac accggccctc cgcgccgggt ccgccgcccg
     6061 ctcccacccc tggcacaccc gggcgaggaa gctgtttcct gggccactct cctcccgcaa
     6121 gagctcgttt ccccggctac catagtagtt gatacccgag acagaaacca tcactccggg
     6181 cggctccggg aggccggcga tggtctccgc gagtaggcgg gtgccctctc tccgactgtc
     6241 catgatgcgc ctcttcttcg ccggcgtcca acgaccttcc ccaacgctct ccgcagctaa
     6301 gtgtacgacg gcgtccaacc ctcccaggcg cgagggttcg agtacgccag agacggggtc
     6361 ccaacgaatt gtatcctccg cggaaggctt agaacggctc agccggatca cccggtgtcc
     6421 gagcctctcg aggtcaagga ctagggccga cccgatgaac cccgtagccc cgctcacgag
     6481 aatgttcatg attttcctcc aacggtcgat gtctgctttg ctcacatggt aacgtcagcc
     6541 ctccctcagg accgtgtgag ctctcccaaa tactcgccct cccgccggat aatgataagg
     6601 gcccagactc gatgccagta gaaccactca cagagcccaa cacctccccg agcgtcgcac
     6661 ctccggacga tggaggtgcg atccgcatcc ttccctcgcc cgatatctcc tcaagtccgc
     6721 atctacttga taaacctaca gctctctgtg ttaccatcca tgtggatggt ctatggatgt
     6781 atagcagatg gggcgtcggc atgagtgaat cggagaagat cacgatcaac cttgggcctg
     6841 tggatctggg gcagatagac ctgctggtgc aggagggctt ctactccaac cggacggact
     6901 tcatccgaac ggccatccgc aaccagctca ccacgcacgc cgaggaggtc aggcagacgg
     6961 tggcgcgcaa gacgctggtg ctcgggctgc agaactacac ccgccgcgac ctggaggcgg
     7021 tacgtgaggc cggagagacg ctggagataa gagtgctcgg cctggcgagc attgacgatg
     7081 acgtagatcc tgagttggcc ctggagacca ttgactccct gatcgtgctc ggcgctctca
     7141 gggcgagccc ggccgtgaag gcggcgctga agggcaggat ccaatcttcc tgacatcgag
     7201 aggagcacta tcatgaacga ccagatgcat accggcatcg ctgaggccac acgcctcacg
     7261 caacagggcc gcctcgacga gg
//
LOCUS       DASTKM010000258         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1147441, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000258 DASTKM010000000
VERSION     DASTKM010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147441"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFQ10_10520"
     CDS             complement(<1..803)
                     /locus_tag="VFQ10_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965817.1"
                     /translation="MRSHLDELRPVLGIVFLAGYVWQTFAPSSLGTDLFLGVGAVLFV
                     ISVPWSSAFHRAFALVAFVALCAVIFTGRFDFADFLGGMPTYFGIVAVLLVLSMAGYP
                     IQAARYEVQIRALMAALTRRGVSIKATAGVLGHVLGAVLDVGAFVLIDVISGRSAPKE
                     RLEALKWAGRAFSFAPLWTNLNVLTATTIVLTGVSYPKLLAVTLPFVLLGLGGTLLFA
                     QRAGGEVEEIPQAPLDRGAAAVLLYPVLLVTAVALVNLAFPKLPLTAAIA"
     gene            1035..2198
                     /locus_tag="VFQ10_10525"
     CDS             1035..2198
                     /locus_tag="VFQ10_10525"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9965818.1"
                     /translation="MQGSARMGGSLDKTVLLAVLFVLLCAACDRSPEESRKSGNDIEP
                     ITTGQGENEEDTGASVERVEVGILATDLEVPWSFAFLPGGDALVTERDSGRLLRVSPS
                     GEVQEIQTLPEGGSGEGGLLGVAVSPDYEDDRYIYAYYTTGVDNRVVRFRMGETPEPI
                     LTGIPSNTYHDGGRIKFGPDGMLYVSTGDAGDSENSQDRSSLGGKILRIEPDGSIPPD
                     NPFPGNPVYSYGHRNVEGLAWDSEGRLYASEFGASTWDEVNRIEAGENYGWPEVEGRG
                     GVDQGYVDPITAWPTSEASPSGAEILVEGAIPQWEGDLFVAALRGERLWRLELNDSGE
                     VVDREKLLDGEVGRVRDVIQAPDGSLWVSTSNYDSFGNPVSEQDDRILRLAAE"
     gene            2209..3507
                     /locus_tag="VFQ10_10530"
     CDS             2209..3507
                     /locus_tag="VFQ10_10530"
                     /inference="COORDINATES: protein motif:HMM:NF044863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965819.1"
                     /translation="MEDTLRSTQDAYPADLARFVRERWDEDRAGALPDQGTLETLISA
                     CYQAGLLHEEERAVTFRVILCEPDLLPPFEGPPSGTHRLEFPKPRQFDVQEIRRLSPA
                     ADYYRALIGVSFDEEEGLRVWGLVHTGPRWLRGGQGGRDVPPPLPPALVLRAYGPGRI
                     AVDIGLEALCKLEEGRLSDTSMDVFDSRWLPATFAPVRGEIAEIHAGLRDQARKKGEV
                     WARLDNDIIRVIGQHTIKRIISAVRDSHHGGTLVIVPPSLAGDILEDRYVSLKYKFVD
                     AEPRRRFRTLIVDVMNALARSYAGRPARDPVGWDDYESSSDRELTGLDEAIFEVAHLI
                     AGLTAVDGAVVMTQRFELLGFGGEISGGLPAVETVARALDVEADAAVEESVGGVGTRH
                     RSAYRLCNELRDVITVVISQDGNVRFVKCKDEAVTYWDQA"
BASE COUNT          693 a         1083 c         1155 g          582 t
ORIGIN      
        1 gcgatggccg ccgtaagcgg aagcttggga aacgccaggt ttaccagggc gacggcagtg
       61 accagcaaga cggggtagag gagaacagcg gcggcccccc ggtccagcgg cgcctgaggg
      121 atctcttcca cctcgccccc cgccctctgg gcgaagagca acgtcccgcc gaggccgagg
      181 agcacgaagg ggagcgtaac cgcgaggagc ttcgggtagg agacgcccgt cagaacgatc
      241 gtcgtggcgg ttaggacgtt caggttggtc cagaggggtg cgaacgagaa ggccctcccg
      301 gcccatttca gggcttccag gcgctctttg ggcgcggagc ggcccgatat cacatctatg
      361 agcacgaacg ctcccacgtc cagaacggcc ccgaggacgt gaccgaggac cccagccgtg
      421 gcctttatac tcaccccccg gcgggtcagc gccgccatca gggcccggat ctggacctca
      481 tagcgggctg cctggatcgg atagcccgcc atgctgagga cgagcaacac agccacgatc
      541 ccgaagtacg taggcatccc cccgaggaaa tccgcgaagt cgaaccgtcc ggtgaagatc
      601 acggcgcata gagccacgaa ggcgaccagg gcgaatgccc tgtggaaggc gctcgaccac
      661 ggtaccgaga tcacgaacaa caccgctcct actcccagaa agaggtctgt ccccaatgag
      721 ctaggcgcga aagtctgcca cacgtacccg gccaggaaga cgatgccgag caccggacgc
      781 aactcatcca gatgcgacct cacgctcccc gcccatgttg cttttcggtg ttctctctta
      841 cctctaaccc gcttccgagt cgccgaaatg gagccagcgc ccacgattct agagcatggg
      901 cgaaggctgt agggagcgct gcggtaacct ctacatcgcc ggaagaatcg agtatgcgct
      961 ccctggctag agggtgggta ccctaccgtg cgcaatataa tcttcgtcga cgtagcagga
     1021 gagaggacgt acacatgcaa ggatctgcca ggatgggcgg ctcgttagat aagactgtgc
     1081 tgctggccgt cctgttcgtt ctcctgtgcg cggcgtgcga ccggtctccg gaagagagta
     1141 gaaagagcgg taacgacata gagccgatca ccacaggtca aggagagaat gaggaggaca
     1201 ctggggcttc cgtggagcgg gtcgaggtgg gtatcctcgc cacggacttg gaggtgccct
     1261 ggtcattcgc gtttctaccc gggggcgacg ccctcgttac cgagcgtgac tcggggaggc
     1321 ttctgcgcgt gagtccatca ggtgaggttc aggagatcca gacgttgccg gagggcggct
     1381 cgggcgaggg tgggcttctc ggcgtcgccg tctcccctga ctacgaggat gaccgctaca
     1441 tctatgccta ctacacgacc ggcgtagaca accgggtcgt gaggttccgc atgggtgaga
     1501 cgccggagcc aatactgacg ggtatcccct ccaacacgta ccacgacggg ggtaggatca
     1561 agttcggtcc cgacggcatg ctctacgtga gcacgggcga tgccggagat tctgagaact
     1621 cccaggaccg gagctctctg ggaggtaaga tcctgaggat agagcccgac ggttccattc
     1681 caccggacaa cccgttcccg ggcaaccccg tctactccta cggccaccgc aacgtggagg
     1741 ggctcgcatg ggactcggag ggaagactct acgcgagcga gttcggcgct agcacctggg
     1801 atgaggtcaa caggatcgaa gccggggaga attacggctg gcccgaggtc gagggcaggg
     1861 gcggcgtgga tcaggggtat gtcgacccca tcaccgcctg gccgacctca gaagcgtccc
     1921 cgagcggggc ggagatcctg gtcgaagggg ccatcccgca gtgggaaggc gaccttttcg
     1981 tggctgctct caggggagag cggctctggc gcctggagtt gaacgacagc ggcgaggtcg
     2041 tggataggga gaagctcctc gacggagagg tcggccgcgt gcgggatgtc atacaggccc
     2101 ccgacgggtc cctgtgggtc tcgacctcca actacgactc cttcggtaac ccggtcagcg
     2161 agcaggacga tcgcatactg cgcctggccg cagaatagaa tgagccctgt ggaagacacg
     2221 ctgagaagta cacaggatgc ctacccggcc gacctcgccc gcttcgtccg tgagcggtgg
     2281 gacgaagata gagccggtgc gctgcccgac cagggtacgc tggagaccct gatctcggcc
     2341 tgctaccagg ctgggttgtt gcatgaggaa gagcgcgccg ttacgttcag ggtgatcctc
     2401 tgcgaacccg atctgctgcc gcccttcgaa gggccgccca gcgggacgca caggctcgag
     2461 ttcccaaagc ccagacagtt cgacgtccag gagataagac ggctctcacc cgccgccgac
     2521 tactaccgcg cgctcatcgg ggtctctttc gacgaggaag aggggttgag agtctggggt
     2581 ctcgtccaca cgggaccgag gtggttgagg ggaggccagg gtggtaggga tgtccccccg
     2641 cccctgccgc ctgccctggt cctccgggca tacggcccgg gacgcatcgc cgtcgacata
     2701 ggtttggaag cactctgcaa gttggaagaa gggaggctct ctgacacctc catggacgta
     2761 ttcgactcgc gctggctccc cgccactttc gcgccggtaa ggggcgagat cgcggagatc
     2821 cacgccggac tccgagatca agcgcggaag aaaggtgagg tctgggcccg cctggacaac
     2881 gacattatcc gcgttatagg gcaacacaca ataaaacgta tcatctccgc cgtacgggac
     2941 tcccatcacg gaggaaccct ggtcatcgtc ccgccgtccc tggccggcga catcctcgaa
     3001 gaccgctacg tttccctcaa gtacaagttt gtcgacgcgg aaccgcgccg gcgcttccgc
     3061 acgctcatag tcgatgtgat gaacgctctg gcccgctctt acgccggcag accggccagg
     3121 gaccccgtcg gctgggacga ttacgagtcg agtagcgaca gggaattgac cgggctcgac
     3181 gaagctatct tcgaggtggc gcaccttatc gccggcctca ccgccgtcga cggtgccgtc
     3241 gtgatgaccc agcgtttcga gctgctcggc ttcggcggtg agatctctgg cggcctacct
     3301 gccgtcgaga cggttgccag agccctggat gtcgaggcgg atgccgccgt cgaagagtcg
     3361 gtgggaggcg tggggacccg ccaccgctct gcttaccggc tctgcaacga gcttcgcgat
     3421 gtcataaccg tcgttatctc gcaggacggc aacgtgcgct tcgtcaagtg caaggacgaa
     3481 gccgtcacct actgggacca ggcgtaggcc gac
//
LOCUS       DASTKM010000259         7089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1498140, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000259 DASTKM010000000
VERSION     DASTKM010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498140"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFQ10_10535"
     CDS             complement(<1..305)
                     /locus_tag="VFQ10_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET9965820.1"
                     /translation="MSRLRTLVDAKSETFAESAERFGVLVDELREISAEARRGGGEKA
                     QRRHRERGKLLVRERIERLLDPGTAFLELSSLAAYGMYDGRVPAAGIVTGVGRVSGR"
     gene            complement(450..794)
                     /locus_tag="VFQ10_10540"
     CDS             complement(450..794)
                     /locus_tag="VFQ10_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HET9965821.1"
                     /translation="MALYALNLFDLADNNLYRQYSRRSVAAVGKHGGMVVALGWLDEA
                     VQTQPGVEPRTVMVLVEWPSRAAFDAFLEDPEHDELHPLREDGTRNYLWWTYERLEDL
                     RPLFGRGSAGGR"
     gene            complement(796..1977)
                     /locus_tag="VFQ10_10545"
     CDS             complement(796..1977)
                     /locus_tag="VFQ10_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9965822.1"
                     /translation="MNFDPTEEQRQWRDLARDFAQGEIRPRAADLDREQKFPYDIVAD
                     MARLGLMGLTLPEEYGGSGGDYVAYNLALEEISRADTSVGITMEAHISLGCAPIAAYG
                     TQEQKETFLSRCATGENLWAFGLTEEEAGTDAGGTRMTAELDSGEWVVNGSKKWITNS
                     GTEITGGVTVIARTGTRENGRPELTAILIPQDTHGYTRGPAYEKLGWRASDQHPLYFE
                     NCRVPAENLLGERGKGLHQFLATLDAGRVAVAALSVGLAQACLDEALARAQRRAQFGR
                     SISEYQAIQFKLADMAMEIELARNLVLKAAWLKDQGRSFGREASMAKVFASEAAKRAA
                     DEAVQIWGGEGFMETSAVARYWRQVKINEIGEGTSEINRQIIARSLLKEGSAKAELLA
                     Q"
     gene            2144..2407
                     /locus_tag="VFQ10_10550"
     CDS             2144..2407
                     /locus_tag="VFQ10_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET9965823.1"
                     /translation="MLGALIFWIIVGLIAGALAKFIMPGDDPGGFIITILLGVAGAIV
                     GGFLASLIGIGGGGFIWTTLIATIGAIILLAIYRAFAGSRAAR"
     gene            complement(2574..4247)
                     /locus_tag="VFQ10_10555"
     CDS             complement(2574..4247)
                     /locus_tag="VFQ10_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9965824.1"
                     /translation="MRPEDIDGLTVGGLLDLVAERRPDHEALVYADRNLRYTYTEFKA
                     VVEDCARSLMALGIQKGDHVAVWGQNVPEWVVLQFATGKAGAVLVTINPAYKSHELKY
                     VLEQSDAVALFLTEGAGDADFLQILQGDVPELVETADGELSIEDLPFLKNVVLMGGDS
                     VEGLPMTGFEEFVVRREEVPDEQLRERQASLDAENVINMQYTSGTTGFPKGVQLTHTN
                     IVKNAFYIGECMRLRPEDRVCIPVPFFHCFGCVLGTLNTVTHEGTMVPVESFDAEKVL
                     QAVDQEQCTALLGVPTMFIAELEHPDFEQYDTSSLRTGIMAGSPCPMEVMKKVVDVMG
                     ASEITIAYGQTESSPVITQTRTDDPLELRVSSVGRKLPDVEVRIVSLETEEDAGPEEQ
                     GELWTRGYHVMRGYYDMEDKTSEVIDDEGWLHTGDLAVMDEDGYVRITGRAKDMIIRG
                     GENVYPREIEEFLYTHPEVSDVQVYGVPDQKYGEKVAAAVKLRQDSELTAEDIKDYCR
                     ENIAYYKVPEYIDFVEDYPMTASGKIQKYKLREAAVERYGLKGTTVESA"
     gene            complement(4330..5268)
                     /locus_tag="VFQ10_10560"
     CDS             complement(4330..5268)
                     /locus_tag="VFQ10_10560"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase"
                     /protein_id="HET9965825.1"
                     /translation="MSSNIAAERAALIVNTRSRSGERTFFKALDRLQELGVPLGVTYA
                     IRDPARLPETVKEVLSDGSGYRFLILGGGDGSVSSVVDFLAHHDKVLGLLPLGTANDF
                     ARTLEIPQDIEGACNTIAHGKVVDIDLGLAGDNYYVNVASVGLGVEATRALSPWLKKS
                     TGPLAYPAAAIKAFLRHEPFSARLTFPEGDHEPVEYDRLLQVAVGNGRFYGGGMVVAP
                     ESGIDDKLLDIYAIDLGRHRNLIGVARYLKSGDFIRMEGVHHFRTSRVQIETDPELAV
                     NIDGEVVARTPQDFSVVQNALHVLVPQESDAARHDE"
     gene            complement(5471..6475)
                     /locus_tag="VFQ10_10565"
     CDS             complement(5471..6475)
                     /locus_tag="VFQ10_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9965826.1"
                     /translation="MSVRVGIVSFAHVHSPAYAGVLKDLGAADFVGIADEDTGRGAEA
                     AEHFGVRFFDSAPSLFEEVEAVVVCSANRNHARDVIPALQGGVHVLCEKPISTTVEDA
                     RAMIQASEDSESQLGTAFPVRYLPSMVRARELVREGALGRVLALNGTNHGQIPGGWFL
                     DPELAGGGAVMDHTVHLADVLRWMLDVEVKSVYAEVDSFFGAGGTDDAAILTLELEGG
                     SFADGAFATLDPSWSRGEGYPTWGDVTLRISGTSGVLDVDPFAQPLRTFDHETGTPSW
                     SYTGEDMNALMLADFLRGVAEGEPSGASGLDGLRTLEVVLAAYRSGEDHEPKKIERRT
                     "
     gene            complement(6472..>7089)
                     /locus_tag="VFQ10_10570"
     CDS             complement(6472..>7089)
                     /locus_tag="VFQ10_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9965827.1"
                     /translation="FFPEYVGIKEKIEAGDLGTVGVVRTSRRSPFLLGWNDWYADWRM
                     SGGVLLDLVIHDFDFLRWALGEVERVYARGMLGREYNRLDYVLVTLRFENGAIAHVEG
                     HWGYPGPFNYSIEVAGSSALVTVDSREPAPLELTGDTATPGEVPDLASGKSPYAKELE
                     HFIQCIATGEEPIVQSQDACEALRIGLAATESVLTGEPVTLGGRE"
BASE COUNT         1240 a         2275 c         2158 g         1416 t
ORIGIN      
        1 tctcgtcccg agacgcgccc aacccccgtg actatccccg ccgcgggaac ccttccgtcg
       61 tacataccgt aggccgccag ggacgagagc tccagaaagg ccgtgcctgg gtcgaggagg
      121 cgttcgatcc tctcccgcac gagcagcttg ccgcgctctc tgtgacgtct ctgcgctttc
      181 tccccccctc cacgtctggc ctctgccgag atctcgcgca gctcgtccac aagaaccccg
      241 aaacgttcgg cgctctctgc gaaggtctcg ctcttcgcat ccacgagggt tcttagcctg
      301 ctcatctttc cccattcggg tggctcgcct ttcgaggcat gttagctacc acttacggca
      361 tgcgccacca tgccgggagg agcgtgcggc gagagggttg gcttagacct cccacggcat
      421 ttaacggtgc tgcgacagta atgacaggat caacgtccgc ccgcggaacc tcgcccgaag
      481 agcggacgca agtcctcgag tcgctcgtag gtccaccaca gatagttccg cgttccgtct
      541 tcccgcagtg ggtggagctc gtcgtgttcg gggtcttcga ggaaggcgtc gaacgccgca
      601 cgtgaaggcc attcgaccag gaccatgacc gtcctcggct cgacacctgg ctgtgtctgc
      661 acagcctcgt ccagccatcc gagcgctacg accattccgc cgtgcttacc caccgcggca
      721 acagaacgtc gtgaatattg gcggtacagg ttgttgtccg cgaggtcgaa gaggttcagg
      781 gcgtagagtg ccattctact gggcgagcag ttcggccttt gcggagccct ctttcagcag
      841 ggagcgggcg atgatctggc ggtttatctc gctggtgccc tcccctatct cgttgatctt
      901 gacctgccgc cagtagcgcg cgaccgcgct cgtctccata aagccctcgc cgccccaaat
      961 ctggacggcc tcatccgcgg cccgcttggc agcctcggag gcgaacacct tggccatgct
     1021 cgcctcccgc ccgaaggagc gcccctgatc cttcagccac gcggccttga gcaccaggtt
     1081 gcgggcgagc tctatctcca tcgccatgtc agcgagcttg aactgaatgg cctggtactc
     1141 cgagatggaa cgcccgaact gcgcccgcct ctgcgcccgc gccagcgcct cgtcgaggca
     1201 cgcctgcgcg agtcccactg agagagcagc gaccgcaacc ctgccagcgt cgagcgtcgc
     1261 caggaactgg tggagccctt tcccgcgctc gccgagcagg ttctccgcag ggacgcggca
     1321 gttctcgaag tagagtggat gctggtctga ggcgcgccag cccagtttct cgtaggcagg
     1381 gcctcgggtg tagccgtgcg tgtcctgggg gatgaggatc gcggtcagct cgggtcttcc
     1441 gttctcgcga gtgccggttc tggcgatgac cgtgacgccg ccggtgattt cggttcctga
     1501 gttggtgatc catttcttcg acccgttgac gacccactcg ccgctatcga gctccgccgt
     1561 catgcgcgtc cctcctgcgt cagtgccggc ctcctcctcc gtcaggccga aggcccagag
     1621 gttctcgcct gtggcgcatc tggacagaaa cgtctccttc tgttcctggg tgccgtaggc
     1681 ggcgatgggg gcgcagccaa gagagatgtg ggcctccatg gttatcccta ccgaggtatc
     1741 ggcgcgtgag atctcttcca gcgccaggtt gtaggcgacg tagtccccgc ccgatccgcc
     1801 gtactcttcg ggcagcgtca gacccatcag gccgaggcgg gccatatccg cgactatgtc
     1861 gtacgggaac ttctgttctc ggtcgaggtc ggcggcacgc gggcgtatct caccctgggc
     1921 gaagtcgcgc gccagatctc gccactggcg ctgttcttcg gtcggatcga agttcacggt
     1981 aatcctcctg ctccctcgga ttgagggcat gatagcagcc gtgcatccgg cacatgtggg
     2041 taccggtcac ggaaacatgt cgtgctttca gaaactaatg tatcctgcgc gcgtattaga
     2101 gtatacactg agacatagaa gaccgaccgt gaaggaggca aggatgttgg gggcactaat
     2161 attctggatc atagtgggcc ttatcgcagg cgcgctcgcc aagttcatta tgcctggcga
     2221 cgatcctggc ggctttatta tcacgattct acttggggta gcgggcgcta tcgtgggtgg
     2281 cttcctggcc agcctcatcg gtatcggcgg tggcggcttt atctggacca ccttaatcgc
     2341 caccatcggc gcgatcatac tgctcgcgat ctaccgggcc ttcgcaggca gtagagcagc
     2401 caggtaacaa agacgggttc gagggccggg atctctaatt ccggcccttt tcttgctgtc
     2461 accgagatca aaccgccaaa ccacaacagc gcgaagtcgc gctgcccaaa cgtggcaagc
     2521 cgggctacag gagttgggat cgccgtagaa cggaactccc ggaagaagct cattcaagct
     2581 gactctacgg tcgtgccttt caggccgtag cgttcgacgg ctgcctcgcg gagcttgtac
     2641 ttctggatct taccgctcgc cgtcatcggg tagtcctcga cgaaatcaat gtactcgggg
     2701 actttgtagt atgcgatgtt ctcgcggcag tagtctttga tgtcttcagc ggtgagttcg
     2761 gaatcctggc ggagcttcac ggctgcggcg accttttcgc cgtacttttg gtctgggacg
     2821 ccgtagacct ggacgtcgga gacctctggg tgagtataga ggaactcctc gatctcgcgc
     2881 gggtagacgt tctcgccgcc ccggatgatc atgtccttcg cccgacctgt gatcctgacg
     2941 tacccatctt cgtccatgac cgcgagatcc cccgtgtgga gccagccctc atcgtcgatg
     3001 acctcgctgg tcttatcctc catgtcgtag tagccccgca tgacgtggta gccgcgggtc
     3061 cacaactcgc cctgctcttc cggcccggcg tcctcctccg tctccaagct gacgatgcga
     3121 acctcgacgt cggggagctt gcgtcctacg ctggatactc gcaactccag cggatcgtcg
     3181 gtgcgggtct gggtaatgac ggggctcgac tcggtctggc cgtatgcgat ggtgatctcc
     3241 gaggcaccca ttacgtcgac gaccttcttc atgacctcca taggacaggg ggagccggcc
     3301 atgatcccgg tacgcaaaga actggtgtcg tattgctcga aatctggatg ttccagctcc
     3361 gctatgaaca ttgtcggtac cccgaggagc gccgtgcact gttcctgatc tacggcctgc
     3421 aacaccttct cggcgtcgaa gctctcgacg gggaccatcg tcccctcgtg ggtgacggtg
     3481 ttgagtgtcc ccagaacgca gccgaagcag tggaagaacg ggaccggtat acacactctg
     3541 tcttcaggcc tgagcctcat gcactcgcca atgtagaagg cgttcttcac gatgttggtg
     3601 tgggttaact gcacgccctt agggaacccc gtcgtccccg aggtgtactg catgttgatg
     3661 acgttctcag cgtcgagcga cgcctgccgc tcgcgtaact gttcgtcggg gacctcctct
     3721 ctcctcacca caaactcctc gaagcccgtc atgggcagcc cttcgaccga atccccgccc
     3781 atcaggacca cgttcttcaa gaaaggcagg tcctctatcg ataactcgcc gtccgcggtc
     3841 tcgacgagct cggggacgtc accttgcagg atctggagga aatcagcatc cccagctcct
     3901 tcggtcagga acagggccac agcgtcggac tgctcgagca cgtacttcag ctcgtgtgac
     3961 ttgtaagcgg ggttgatagt aaccagcacg gccccggctt tgcccgtggc gaactgcagg
     4021 accacccact caggcacgtt ctggccccac acggcgacgt ggtcgccttt ctgtataccg
     4081 agcgccatca atgacctggc gcagtcctcg acgaccgcct tgaactccgt gtaagtgtaa
     4141 cgcaggttcc tgtcagcgta gacgagagct tcatgatcag ggcgtcgctc ggccaccaga
     4201 tccagcagcc cgcctacggt caaaccatcg atatcctcag ggcgcatttc ttcctcctca
     4261 tcgcggtccc caccgatgtt ccgcagtcta gcaatcgagg ccggccacgc aagcgcgcct
     4321 gtcgctgccc tactcgtcgt gacgcgcggc gtcggactcc tgcggcacca gaacatggag
     4381 ggcgttctgg accacagaga agtcttgcgg ggttcttgcc accacttccc cgtctatgtt
     4441 gaccgcgagc tcggggtccg tctcgatctg gacgcgcgag gtcctgaagt ggtgaacgcc
     4501 ctccatcctg atgaagtccc cgctcttgag gtatctagcg accccgatga ggttcctatg
     4561 ccggccgaga tctatagcgt agatgtcgag gagcttgtcg tctataccag attcgggggc
     4621 gacgaccata cccccgccgt agaagcgccc gttgccgacg gcgacctgca gcagacggtc
     4681 gtattcgacg ggctcgtggt caccttcggg gaaggtcagc ctggcagaga agggttcgtg
     4741 cctcaggaac gccttgatag cggcagcggg ataggccagc ggacccgtgc ttttcttcag
     4801 ccacggagag agagcccgcg tggcctcaac cccgagcccg acggaggcca cgttcacgta
     4861 gtagttgtcg ccagcgagac cgaggtctat gtcgacgacc ttgccatgcg ctatcgtatt
     4921 gcaggcgccc tcgatgtcct gcggtatctc cagcgtcctg gcgaaatcgt tggccgtacc
     4981 gaggggcagg aggcccagaa ccttatcgtg gtgcgccagg aagtccacga cggagctcac
     5041 tgacccgtcg ccgccgccca ggattagaaa cctgtagccg gaaccgtcgc tgaggacctc
     5101 cttcaccgtc tcggggagcc gcgcgggatc ccttatagcg taggttacgc cgagggggac
     5161 accgagctcc tgaaggcggt cgagggcctt gaagaacgtg cgctccccgc tgcgggaacg
     5221 cgtgttgacg atcagggccg cacgctcggc cgcaatgtta ctgctcatac gaaatcctct
     5281 agatcgcttg tggttggaag cgcggagatc ctgccgaagt ggattccctc ttgcgcggga
     5341 atgacgcgag atgaggcggc aatgacgaac gctctcgttc ttgcgagcaa ggggaatgag
     5401 atcaccataa caaagagagt atacgagtcc ctggcccaca gggtttcatc ggatggcacg
     5461 tcgaaggcgg tcaggtcctc cgctctatct tcttcggttc gtggtcctct ccggaacggt
     5521 atgcggccag gacaacttcg agcgtccgca ggccatcgag gccgctcgcc ccagacggtt
     5581 caccctcggc cacgccgcgc aggaaatccg ccagcatgag cgcgttcatg tcctcacccg
     5641 tgtatgacca tgaaggggtg cccgtttcgt ggtcgaaggt tctcaatggc tgtgcgaacg
     5701 ggtccacgtc gaggacgccg gaggtgcccg agatcctgag ggtcacgtcg ccccaggtgg
     5761 ggtaaccttc gccgcgggac caactcgggt ctaaggtggc gaaggcgccg tcggcgaagg
     5821 agccgccttc gagctccagg gtcaggatgg ctgcgtcgtc cgtccctcct gctccgaaga
     5881 acgaatctac ctccgcgtag acgctcttca cctcgacatc gagcatccag cgcagaacgt
     5941 ctgcgaggtg gacggtgtgg tccatcaccg cgccgccgcc agcgagctcg ggatcgagga
     6001 accatccgcc tgggatctgg ccgtggttcg tgccgttcag ggccaacacg cgtcccaagg
     6061 ctccctcgcg cacgagttcg cgcgcgcgga ccatggaggg gaggtagcgt acagggaagg
     6121 ccgtcccgag ctggctttcc gagtcctcgg aggcctggat catggccctg gcgtcctcga
     6181 cggtggtcga gatgggcttc tcacagagca cgtgcacgcc gccctggaga gcggggatca
     6241 catccctcgc gtggttcctg ttcgcagagc agacgacgac ggcctcgact tcctcgaaga
     6301 gggagggcgc gctatcgaag aaccgcacgc cgaaatgctc ggcggcctcg gcgccgcgac
     6361 ctgtgtcttc gtctgctata ccgacgaaat ccgcggcgcc gagatctttt agcacgccgg
     6421 cgtacgccgg agaatggacg tgggcgaagc tgacgatgcc gaccctcacg ctcactcccg
     6481 gcctcccagc gtaacgggtt ctcctgtcag gacggattca gtcgcggcga ggccgatcct
     6541 gagcgcctcg catgcgtctt gcgactgcac gataggttct tctccggtcg ctatacattg
     6601 gatgaagtgt tcgagctcct tcgcgtacgg gctctttcct gaggcgaggt cgggaacctc
     6661 gcctggggtc gccgtatcac cggtaagctc cagtggggcg ggctccctgc tgtccaccgt
     6721 aacaagggcg ctgctccccg caacctcgat ggagtagttg aaaggacctg ggtaccccca
     6781 atgcccctcg acgtgggcga tggcgccgtt ctcgaagcgc agggtgacga ggacgtaatc
     6841 gaggcggttg tactcgcgcc ctagcatgcc gcgggcgtag acccgctcga cctcgccgag
     6901 agcccagcgc aggaagtcga aatcgtggat caccaggtcc agtaggacac cgccgctcat
     6961 gcgccagtcg gcgtaccagt cgttccagcc gagcaggaag ggtgagcggc ggctggtgcg
     7021 gaccaccccg accgtcccga ggtctccggc ctcgatcttc tccttgatcc ccacgtactc
     7081 ggggaagaa
//
LOCUS       DASTKM010000260         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_440358, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000260 DASTKM010000000
VERSION     DASTKM010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440358"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFQ10_10575"
     CDS             complement(<1..363)
                     /locus_tag="VFQ10_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965828.1"
                     /translation="MARRTLYLPVVIAAALAMACLVALLAVSQKAEATFPGKNGRIAF
                     EGDRDTGTGGSAAEIITVSPDGTGMKQLTSASGSGNPTFSPNGEKIAYAANYGAGTRG
                     IYVMNRSGNAKKALTDSNN"
     gene            complement(482..613)
                     /locus_tag="VFQ10_10580"
     CDS             complement(482..613)
                     /locus_tag="VFQ10_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965829.1"
                     /translation="MDRMQEGLLALLLMVLMRLARSRAVVGDILCGERRKFKGEPSA"
     gene            complement(825..1493)
                     /locus_tag="VFQ10_10585"
     CDS             complement(825..1493)
                     /locus_tag="VFQ10_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965830.1"
                     /translation="MREEFMITRQGKQYVLYSGLLDEAHSLGLTGIDTDLIQVPDESN
                     GNVAIVKATAEMEDGRRFSGIGDASPENVGRNIAPHLIRMAETRAKARALRDAVNVGA
                     TALEELSDGDDAPPASQSSSSRGRPTPIRGGRQNSSQTSSQSAAAEQDTPNGQAEKPA
                     SAKRGAGKARKSQVDLLKTLAVEWRGENGVERLENRLGKPLKDMTRAEADEWIDRLTP
                     EGRE"
     gene            complement(1490..2320)
                     /gene="zapE"
                     /locus_tag="VFQ10_10590"
     CDS             complement(1490..2320)
                     /gene="zapE"
                     /locus_tag="VFQ10_10590"
                     /inference="COORDINATES: protein motif:HMM:NF005992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HET9965831.1"
                     /translation="MSGSLGRRSKRETGAEVLRLDDKICPHCGRELQAEWDEFPPALQ
                     KKYGKPGEWYYHPCTPECEKKNEQREWELMRRDARVATLRDRSGLSKRMKDYALDNFD
                     PGFSKNTQKAFAKVMDYIMGWKANQEGGRGLYFCGPVGVGKTHLAVAVMNEIITKKRV
                     PSLFVTVPELLDNMRGAYNDPGRDIDEWMDSVKNADLLLLDDLGAERANEWVRERIFV
                     IVNHRYREELPTIFTSNTGPKDLAAQLGERTASRIIAMCDWISLEGEDYREAEVRRGL
                     "
     gene            complement(2292..>2553)
                     /locus_tag="VFQ10_10595"
     CDS             complement(2292..>2553)
                     /locus_tag="VFQ10_10595"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET9965832.1"
                     /translation="ASERDARRVGYVRSILERWVERGVKTVEDVERFEQDRKLRLVEQ
                     SGAVRGGALRIREAREGVSGGPDRQSAARRREGYEWLFGETE"
BASE COUNT          379 a          870 c          753 g          551 t
ORIGIN      
        1 gttgttggaa tctgtgaggg ccttcttagc gttgccggag cggttcatca cgtagatgcc
       61 gcgggtgccg gctccgtagt tggcggcata ggctatcttc tcgccgttgg gcgagaaggt
      121 ggggttgccg gagcccgagg cgctggtgag ctgcttcatc cctgtgccgt ctggggacac
      181 ggtgatgatc tctgcggcag agccaccggt gcccgtgtcg cggtcgccct cgaaggctat
      241 cctgccattt ttgcccggga aggtagcctc tgccttctgt gagaccgcca acagggcgac
      301 caagcatgcc atcgccagcg cagccgcgat cacaaccggt agatagagcg tccgtcttgc
      361 catgtggatc tcccctcttc gtcacgtaac acgacgtgag tataaccgca gggacagggt
      421 agcatatagg ccgcatgacc catctcgacc tttgcgacct tccatgagaa ggcacatttt
      481 gtcaagccga cggctctccc ttgaacttgc gacgttctcc gcagaggatg tctccaacca
      541 ccgcccggct ccgcgcaagc cgcatcagga ccatcaacaa cagcgcgagg aggccctcct
      601 gcatcctatc catcagcctt ccacgcgtac gccacgtcct ccccggtcgg gtggactata
      661 cggctcatct ttggcaagat ctctaccgat gtcgctcgct tggggatact gctcaaagat
      721 cgaccgctgg ccgcgagtct ttgaatgcga gcttggccca tccatgttgt ggatcgacag
      781 gctggtggtt tccaggttgg cgcatcggac cgtgccccag ccggttattc gcgcccttcc
      841 ggggtaaggc ggtcgatcca ctcgtcggcc tcggcgcggg tcatgtcctt gagcggcttg
      901 cccaggcggt tctccaggcg ctcgacgccg ttctcgcccc gccactcgac cgccagcgtc
      961 ttcaggaggt cgacctgact cttgcgcgcc ttgcctgccc ccctcttcgc ggaggccggt
     1021 ttctccgcct gtccgttcgg ggtgtcctgc tctgcggcag cactctggga ggaggtttga
     1081 gaggagttct gtctcccccc gcggatggga gtgggcctgc cacgcgatga cgagctctgg
     1141 gaggcaggag gggcgtcgtc gccgtcggag agctcctcga gggcggtcgc gccgacgttc
     1201 acggcgtcgc gcagggctcg ggctttggcc ctggtctctg ccatccggat caggtgtggg
     1261 gcgatgttgc gcccgacgtt ctcggggctc gcgtccccga tacccgagaa gcggcggccg
     1321 tcctccatct cggccgtagc cttcactatg gcgacgttcc cgttcgactc gtcggggacc
     1381 tggatcaggt ctgtgtctat ccccgtcagt cccaggctat gggcctcgtc gaggaggccc
     1441 gaatagagga catattgctt tccctgacgg gtgatcatga actcttctct cacaggccgc
     1501 gccttacttc ggcctcgcgg tagtcctcgc cttccaggga gatccaatcg cacatcgcga
     1561 tgatcctgct cgccgtcctc tccccgagct gggccgccag atccttcggt cccgtattgg
     1621 aggtgaagat agtcggcagc tcttccctgt agcggtggtt gacgatgacg aagatgcgct
     1681 cgcgcaccca ctcgttcgcc ctctcggcgc cgaggtcgtc gagtagcagc agatccgcgt
     1741 tctttacgga gtccatccac tcgtcgatgt cccggcccgg gtcgttgtaa gccccgcgca
     1801 tgttgtccag aagctcgggt accgtgacga agagcgaggg cacacgcttc ttggtgatga
     1861 tctcgttcat gacggctacc gcgaggtgcg tctttcctac gcccacaggc ccacagaagt
     1921 acagcccccg cccaccctcc tgattggctt tccagcccat gatgtagtcc atgaccttgg
     1981 cgaatgcctt ctgggtgttc ttgctaaatc ctggatcgaa gttgtcgagc gcgtagtcct
     2041 tcatgcgctt gctgaggcca ctacggtccc tcagggtcgc gacccgcgcg tcgcggcgca
     2101 tcaactccca ctcgcgctgc tcgttcttct tctcgcactc cggagtgcac ggatggtagt
     2161 accactcacc aggcttcccg tacttcttct ggagggccgg cgggaactcg tcccactcgg
     2221 cctggagctc gcgtccgcaa tgggggcaga tcttatcgtc gagcctcagt acctcggccc
     2281 cggtttcgcg cttactccgt ctccccaaag agccactcat agccctcccg gcgtctggca
     2341 gcagactgtc tatccggtcc accactcacc ccctctctgg cttcgcgtat cctcaaggcg
     2401 ccgcccctca cagccccgct ctgctcgacg agcctgagct tgcggtcctg ctcgaagcgc
     2461 tccacgtcct cgacggtctt cacgccccgc tcgacccacc gctccaggat gctccgcacg
     2521 tagccgaccc tgcgcgcgtc gcgctcgctg gct
//
LOCUS       DASTKM010000261         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_268536, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000261 DASTKM010000000
VERSION     DASTKM010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268536"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VFQ10_10600"
     CDS             complement(<1..986)
                     /locus_tag="VFQ10_10600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET9965833.1"
                     /translation="MRSVETGAEAARVPPHDLDAERAVIGAMLVSETAVAAVAERLAA
                     EDFYSEVHRIIYGAMMRLYSRGEPIDQLTLTNELRSVNEFERIGGRPYVFQIVESVPT
                     AANAGRYADIVRGKALLRAIIDVGSRITEDAFREPEDVTEALDSAEQLVYGVSNRTLR
                     EHLAPVSELAPGALEMIQRLYEQEGEVTGVETGFEDLDRLTTGFHNSDLIILAARPAM
                     GKTALSLNAIWHAASEKKMPVAIFSLEMSKEQLVQRLISQTTRIPTQALRSGNVKAED
                     WPKLVRGVAEVSRAPIWIDDTAGVTLMEIRAKVRRLASQLNVAGEMPLSLVVV"
     gene            complement(1140..1589)
                     /gene="rplI"
                     /locus_tag="VFQ10_10605"
     CDS             complement(1140..1589)
                     /gene="rplI"
                     /locus_tag="VFQ10_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET9965834.1"
                     /translation="MQVILNQDVEKIGQRGDIVDVSRGYVRNFLVPRGLAEVATPAKL
                     EEARRRMEESEERDRRLAERAEEISGILNKSVITIEARTGEDERLFGSVTAANIADAV
                     ERARGIRLDRRRIRLEEPIKALGTHQVPIQVHGEVEASVKVIVVPKL"
     gene            complement(1595..1804)
                     /gene="rpsR"
                     /locus_tag="VFQ10_10610"
     CDS             complement(1595..1804)
                     /gene="rpsR"
                     /locus_tag="VFQ10_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET9965835.1"
                     /translation="MKSKPCVFCKENVAYADYKDYNMLRRFISDRGKVRARRVTGLCP
                     QHQREAASAIKRAREMALLPYVVGR"
     gene            complement(1865..2236)
                     /gene="rpsF"
                     /locus_tag="VFQ10_10615"
     CDS             complement(1865..2236)
                     /gene="rpsF"
                     /locus_tag="VFQ10_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET9965836.1"
                     /translation="MAGEADTRPEEANIDTYEAMLIVIPELDEEQVENTVGRFRTIIE
                     RTGGEAGEPNHWGRRKLAYEIDHRTDGFYVVMEFTAGERTLVELKRILRVSDDVLRHM
                     VVKLPPSYAHKPIPEPTEVTA"
     gene            2353..>2789
                     /locus_tag="VFQ10_10620"
     CDS             2353..>2789
                     /locus_tag="VFQ10_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9965837.1"
                     /translation="MENGAGIAVETMGLEKVYGEGLTAVRALQDVSLAFENGEFAAIM
                     GPSGSGKSTLLHILGALDKPTAGRVVVGGTDLSGLSDKKLTLLRRERMGFVFQFFNLI
                     PTLTAEENVLLPVLIAGKRPGNYGKRLDELLDLVKLKGRRTHKP"
BASE COUNT          457 a          901 c          858 g          573 t
ORIGIN      
        1 acgacgacga gggagagagg catctctccg gcgacgttca gctggctggc gagacgcctg
       61 accttggccc ggatctccat gagcgtgacg cccgccgtgt cgtcgatcca gatcggggcc
      121 cggctgacct ccgctacccc ccttaccagc ttcggccagt cctcggcttt cacgttgccg
      181 ctcctcaacg cctgcgtagg gatgcgtgtg gtctgcgaaa tgagcctctg gacgagctgt
      241 tctttactca tctccaggga gaagatcgca acaggcatct tcttctcgct agcggcgtgc
      301 cagatcgcgt tgagagacag cgcggttttg cccatcgccg gtctcgcggc gagtatgatc
      361 aggtcgctgt tgtggaagcc cgtggtgagg cggtcgagat cttcgaagcc tgtctcgacc
      421 cctgttacct cgccctcctg ctcgtagagg cgttggatca tctccagcgc accgggcgct
      481 agctcggaga cgggcgcgag atgttccctg agcgtccggt tggagacacc gtagacgagc
      541 tgctcggccg agtcgagcgc ctcggtcacg tcctcgggct ccctgaaagc atcctcggtg
      601 atgcggctcc ccacatctat gatcgctcgc aagagcgcct tgccgcgcac gatgtcggcg
      661 tacctgcccg cgttcgcagc cgtcgggaca ctctcgacga tctggaagac gtagggcctg
      721 ccgcctatcc tctcgaactc gttgacgctt ctaagctcgt ttgtgagcgt gagttgatct
      781 atgggctcgc cgcgcgagta cagccgcatc atggccccgt atatgatcct gtgcacctcg
      841 gagtagaagt cctcggcggc gagccgctcc gcgaccgcag ccacagcagt ctccgaaacc
      901 agcatcgcgc ctatgactgc ccgctccgca tccaggtcat gcggcggtac acgggccgcc
      961 tcagcccccg tctctacact gcgcatcctc ccccggcgtt ccataaccta ccagcatgca
     1021 gcaaaaaggc ccccgttaca agacgggagc cttgatcttc tggttcagct tgtgcctaaa
     1081 cgtgcgcttg aggtgaagac gtttcgatgt agatgtaggg gcgggtttca aacccgcccc
     1141 tacagtttgg ggaccacgat aaccttgacg ctcgcttcga cttcgccgtg gacctggatg
     1201 ggcacctggt gcgtacccag ggcctttatg ggctcttcga ggcggatgcg gcgtctgtcg
     1261 aggcgaatac cgcgggccct ctcgaccgcg tcggcgatgt tggctgccgt gaccgaaccg
     1321 aaaaggcgct cgtcctcgcc cgttcgggct tctatggtga tcacgctctt gttgaggatg
     1381 cctgagatct cctcggccct ctcggccaga cgcctgtctc gctcctccga ctcctccatt
     1441 ctgcggcggg cttcctccag cttggcgggg gtcgccacct cggcgaggcc gcgcgggacc
     1501 aggaagttgc gcacgtaacc gcggctaacg tcgacgatgt cgccgcgctg cccgatcttc
     1561 tcgacgtcct ggttcagtat tacctgcacg gctcctacct ccccaccacg tagggcaaga
     1621 gcgccatctc cctggcgcgt ttgatcgccg aggcggcctc ccgctggtgc tgcggacaca
     1681 ggcccgtaac cctacgggcc ctgaccttgc cacggtcgga gatgaaccgc cgcagcatgt
     1741 tgtagtcctt gtagtcggcg taagcgacgt tctccttgca gaacacgcaa ggtttgcttt
     1801 tcatcggtcg ccccccgcgg ggcggcccgt ttcttctcct acccattcgt tacctccgga
     1861 ttagtcaagc cgtaacttcg gtgggctcag ggattggctt gtgcgcgtag ctcggcggga
     1921 gcttcacgac catgtgcctt agcacgtcgt cggagacgcg caggatacgc ttcaactcga
     1981 cgagggtgcg ctcgccggcg gtgaactcca tgaccacata aaaaccgtcc gtgcggtggt
     2041 ctatctcgta ggccagcttg cggcggcccc agtggttcgg ctccccggcc tcgccgcccg
     2101 tgcgctcgat gatcgtacga aaacgcccga cggtgttctc gacctgctcc tcgtcgagct
     2161 cggggatcac tatgagcatg gcttcgtaag tgtcgatgtt tgcctcctcc ggtctggtat
     2221 cggcttcccc ggccaaggcc agtggggaag caggggccta ttagatttcc aggatagtgt
     2281 aacagtggga tcgaactgtt acaaacgacg ttacggcagt ctctctgtcc ggggtgggga
     2341 tatactctgc gcgtggagaa cggagccggc atcgcggtgg agaccatggg cctggagaag
     2401 gtctatggag agggtttgac cgcggtgcgt gccctccagg acgtctccct ggcgtttgag
     2461 aacggggagt tcgcggccat catgggccct tcgggatcgg gcaagagcac gctcttgcac
     2521 atcctcggcg ccctggacaa gcctacggcg gggcgcgtag tcgtcggggg gaccgacctc
     2581 tcggggctct cggacaagaa gctaacgctc ctcaggcgag agcggatggg cttcgtgttc
     2641 cagttcttca acctcatccc cacgctcacg gccgaggaga acgtgctctt gcccgtgctc
     2701 atcgccggca agagaccggg caactacggc aagaggctcg acgagctcct ggatctcgtg
     2761 aagctcaagg ggcgtcggac gcacaagcc
//
LOCUS       DASTKM010000262         7646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1500135, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000262 DASTKM010000000
VERSION     DASTKM010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500135"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VFQ10_10625"
     CDS             complement(<1..883)
                     /locus_tag="VFQ10_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9965838.1"
                     /translation="MLDAKKPWLSIYGDRLSGEPLGGSLTGFLEEAVEKYGDNTALTR
                     GEQKISYGELLDLTESFAAALYEAGVQKGDRVGLMLPNCPEYVVAFFGTMRLGATVTQ
                     VNPIYVGRELTHIFDNSGTETVIVHSMMYEKAKAVQAATPLQRIICVGEPEGGLKDGD
                     APFDEFLHSATGSVPEVEIDPQEELSSIQYTGGTTGISKGAMLTHANLLGGVKQTMDL
                     LIEDPAAFPENGKVIAVAPFFHIFGMTMVLLFGLRMGWNLLTVPRFQPDVVMEMIRDE
                     EPVMLPGVATLFVALHNY"
     gene            complement(1025..2638)
                     /locus_tag="VFQ10_10630"
     CDS             complement(1025..2638)
                     /locus_tag="VFQ10_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HET9965839.1"
                     /translation="MKTVLPGQGFRFELDGRSLTIEDVVVLSRRSEYASCALSAEAVE
                     KIMATRALKQDLIGREVPIYGVSTGFGDSAHRQISASKTARLQQNMLRFLGSGTGPIA
                     SPEVTRAAMLLRANCLARGNSGVRLELVERLLFYLNNDVLPLIPERGSCGASGDLVPL
                     SYVGAALVGEVEVLHDSEVKDSKELLSELGLEPLELEAKEGLALTNGTSFMSAFAALA
                     VWDAEELALVSDLCTAMASEALLGNRGHFNAFIHEQKPHPGQVESARVIRNLLADSEL
                     ALDSEEIFAGDGLGGKEFLELDRQVQDKYSIRCAPHVSGALRDTLEWVRRWVEVEINS
                     SDDNPLFDAASGRVQSGGNFYGSHITQAMDSLKIAVANLCDLMDRQLELVVDEKFNAS
                     LTPNLIPFCDEDSPEAGLHHGFKGMQLCCSAMTAEALKISNPASVFSRSTEAHNQDKV
                     SMGSIAARDARSIIELAQNVAAIHLLAVCQALDLRGAQKASPKTRAVHELVRSRVPFL
                     DGDRRMDTDIKEIVDLIRSGELSKRALPT"
     gene            complement(2823..4577)
                     /locus_tag="VFQ10_10635"
     CDS             complement(2823..4577)
                     /locus_tag="VFQ10_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HET9965840.1"
                     /translation="MLASAEEHARLVETARGDRPADLFVRGGTIANVYSGEFYEGNVA
                     VTAGRIAFVGEGERTVGQDTEIVEAKGMIVAPGYIEAHFHPWVRYNPVSLVEGVLPLG
                     TTAIVADNLFFFMQMGHEGFAKMAGDLRDLPLLYLWMARLTSQAAFPGEEEMFGLEKV
                     ELLLRRDDVLGTAEVTRWTALAAGDPALISGIRKAKSLGKIVDGHTGGASESRLQPVA
                     AAGIDADHEAITREEVLNRLRLGIWTMLRNSSLRPDLPELLRSVTENGVSTHRLIMTT
                     DGPSPEFIAEHGLVDGMLRVAVDNGVSPMQALQMVTINPATLFGIDGQVGGIGIGRRA
                     DLLLLPDLVSFRPLTVITRGHVVADRGELRVPLPDIEWDAYGSRPRFEPDLDLGDPAL
                     YPQKPSEAEVPVIHLRSAVITERKDARVRSDNGLVVPPEGALHAALIDREGAWISRAL
                     VSGYAANLDGLASTYNTTTHLLVLGRRPQSIARAARRVRELNGGIVVVEDEKVTFELA
                     LPVSGMMSGLPFADVAELNRRLSRAVARAGYEHHDILYTLLFLTCDFLPALRLTPLGL
                     LDVKSSETLVASERLKTV"
     gene            complement(4583..5581)
                     /locus_tag="VFQ10_10640"
     CDS             complement(4583..5581)
                     /locus_tag="VFQ10_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9965841.1"
                     /translation="MSVAPSSLGTQPIGETSRVSDDIFQVKLPVPFPLKFVASYLVED
                     CDGWTVIDPGFDYPPARETWERGAAKVGLDLERDLARIIVTHLHPDHIGLARWLEERS
                     GAPVWMLEGEIENARRVWDPDRGTEDFVRYLIRNGMDAQTAEPTAGSTRLGVRLPERL
                     LPLRPGDSIELGASEARVVHTPGHSDFHFILHDERQRLLFAGDHLLLEITPNIGLWTY
                     TAPRPLRRYLDSLASLRGLDVDLVFPGHGPLFHDLDGRVDELLAHHEERLSVMLAALD
                     GRPATPFEVARRVFPEDLTDHQLRFALAETLAHLEHLEGEDYVQRVADGVVSYQAV"
     gene            complement(5592..6227)
                     /locus_tag="VFQ10_10645"
     CDS             complement(5592..6227)
                     /locus_tag="VFQ10_10645"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9965842.1"
                     /translation="MATSDTLIRSSDREGEALAPKQARLIRSAYKVMGEKGLRHLSLQ
                     DVADEARVSKAILPYYFESKENLILLTMRWVLARVAGRIREATAEAPSAEEKVSAMID
                     AIFVSPESNRRFYLVFFDFLGYAARNDRFADVGATFHEICNGLYAEVIELGQEEGVFL
                     GGDSREGATVVRALVDGLFTQWIQEKDWEKTHAEYREICKRSVLTYLTAGA"
     gene            complement(6283..7053)
                     /locus_tag="VFQ10_10650"
     CDS             complement(6283..7053)
                     /locus_tag="VFQ10_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9965843.1"
                     /translation="MAVEYTREGSVGYITLDNPPANSYDLSFMEELSECVRSAAEDEG
                     TGAVILRSGSERFFSAGADIKAFDANSVEDNMRMISQAHEGLSEIAHTPKVFIAQIGA
                     TALGGGLELALACDLRFGAEGEYVLGLPEATLGLLPGNGGTQRLPRLIGVSKALDLMV
                     TGRTISPSEAHEVGILDRLFPADALEEETRKYAEKLAGGATEAIGSIKLAVYGGIERP
                     LDEGLETERTLVEPLFESEDAREGISAFMEKRKPEFSK"
     gene            complement(7136..>7646)
                     /locus_tag="VFQ10_10655"
     CDS             complement(7136..>7646)
                     /locus_tag="VFQ10_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9965844.1"
                     /translation="DGMEKAEKPKIRMGPLNAAKHRDVLADQLQDAVDGGAKVAVGGN
                     GHEGKGYFFEPTVIEDAPHDSRVVREETFGPLLPVFKVNDVDEAIRLANDSAYGLGSS
                     VYTYNVKWIHRAAQEIEAGMTWVNQLHFGYDELPFGGVKDSGIGREHGKEAIEHYLEQ
                     KSVVVGGLD"
BASE COUNT         1375 a         2474 c         2322 g         1475 t
ORIGIN      
        1 gatagttgtg tagggcgacg aagagcgtcg ccacgccagg aagcatgacg ggctcttcgt
       61 cccggatcat ctccatcacg acgtctggct ggaatcgggg gacggtgagc aggttccatc
      121 ccatccgcag cccgaatagc agcaccatag tcatcccgaa gatgtggaag aaaggcgcta
      181 cagcgatcac ctttccgttc tccgggaaag cggcggggtc ctctatgagc aaatccatcg
      241 tctgcttgac gccgccaagt agattggcgt gcgtgagcat agcgccctta gagattccgg
      301 tggtgccacc cgtgtattgt atggaggaaa gctcttcttg cggatctatc tccacctctg
      361 gcacgctccc cgtagcggaa tgcaaaaact cgtcgaaagg cgcgtcgccg tccttcaagc
      421 caccttccgg ctcgccgaca catatgatcc tctgcagcgg cgtagccgcc tgaacggcct
      481 ttgctttctc gtacatcatg gaatgaacga tcacggtctc cgtgccggag ttatcgaaga
      541 tgtgcgtgag ctcccggcca acgtagatcg ggttgacctg cgtgaccgtc gccccgagcc
      601 tcatcgtccc gaagaacgcg acgacgtatt ccggacaatt aggaagcatc agtcccacac
      661 ggtcgccttt ctgtactccg gcttcgtaga gcgcggcggc gaagctctcg gtcagatcca
      721 gaagctcgcc gtacgagatc ttctgctcac cccgagtcag ggccgtgtta tccccgtact
      781 tctctaccgc ctcttctaga aaccccgtca gcgaaccgcc caaaggctca cccgagagcc
      841 tgtctccgta gatcgagagc cagggcttct ttgcgtccaa catggtcacc acctccgcgt
      901 gcttgcaccg cacatgtcgt tcagcatagc cttcgcctgt tcttgatcaa ctgctacacg
      961 atattgccaa cttccccgac catgaccgtc gcttcagcac accgcgagcc gaacagagaa
     1021 ccgctcaggt cgggagagcc ctcttcgaga gctcgccgga ccggatgagg tctacgatct
     1081 ccttgatatc cgtgtccatc cgccggtcgc catccaggaa aggcacgcga gagcgcacga
     1141 gctcgtgtac cgccctggtt ttcgggctcg ccttctgtgc gccacgcagg tcgagtgcct
     1201 ggcagacggc gaggaggtgt atagccgcga cgttctgcgc gagctcgatg atggatctgg
     1261 catcgcgggc ggctatcgag cccatgctca ctttgtcctg attgtgcgcc tcggtggagc
     1321 gggagaagac gctcgccggg ttggagatct tgagcgcctc ggctgtcatc gccgagcagc
     1381 agagctgcat ccccttgaaa ccgtggtgca ggcccgcctc ggggctgtcc tcgtcgcaga
     1441 aagggatcaa gttcggagtc aggctcgcgt tgaacttctc gtccacgacc agctcgagct
     1501 gccggtccat caggtcgcag aggttggcta cggcgatctt tagcgagtcc atagcctggg
     1561 tgatgtggct gccgtagaag ttgcctccgc tctgcacccg gccgctcgcg gcgtcgaaga
     1621 ggggattgtc gtcggaggag ttgatctcga cctctaccca gcgccttacc cactccagtg
     1681 tgtcgcggag agcgccgctc acgtgtggcg cgcaacgaat agaatactta tcctgcacct
     1741 gcctatccaa ctccaggaac tccttgccgc cgaggccgtc gccggcgaag atctcctcgg
     1801 aatccagcgc cagctcggaa tctgccagca gattccggat gacacgtgcg ctctcgacct
     1861 gaccggggtg cggcttctgc tcgtggataa aggcgttgaa atgtccccgg ttgccaagca
     1921 acgcctcaga cgccatcgcc gtgcacaggt ctgatacgag cgccagctcc tccgcgtccc
     1981 acaccgccag agcggcgaac gcgctcatga acgaggtccc gttggtcagg gcgaggccct
     2041 ccttggcctc gagctccagc ggctccaaac ccaactcact caaaagttcc ttcgagtcct
     2101 tcacctcgct atcgtgcagg acctccactt cgccaacgag agcggcgccc acatacgaaa
     2161 gcggcaccag gtctccgctt gcgccgcagg agccccgctc aggaatgagc gggagtacgt
     2221 cgttgttcag gtagaagagg aggcgctcga ccagttctag gcgaacaccg gagttgcctc
     2281 tggcgaggca gttggcgcgc aggagcatcg ctgccctcgt aacttccggc gaggcaatcg
     2341 ggcccgttcc gctgcccagg aagcggagca tattctgctg cagacgggcg gtcttggacg
     2401 cggaaatctg ccggtgcgcg ctgtccccga agcctgtcga gacaccgtag atcgggacct
     2461 cgcgcccgat cagatcctgc ttcagcgcac gcgtcgccat gatcttctcc accgcctcag
     2521 cggatagagc gcacgacgca tattcactcc tgcgggataa aacaaccacg tcctcgatcg
     2581 ttagcgatct cccgtcgagc tcaaaccgga agccctgtcc cggtagaacg gtcttcatcg
     2641 cgtatcaccc cttgtgctgg ccgtgtttcc ccacgtcctg gcaccgattg accggtcgga
     2701 cagctagtat tttaactcgg ttcgccccag gaaacatgcc gagacggact tcctcagccg
     2761 accccacgaa gtcacacgca ggctcggttc tggcgtcgac gacggcggct tgcgcagtaa
     2821 ggtcatacgg tcttcagacg ctcggaggcc accagtgtct cggaagattt cacatcaagc
     2881 aggcccaggg gtgtgagtcg gagtgctggc aggaaatcgc acgtcaagaa gaggagcgtg
     2941 tagaggatgt cgtggtgctc gtatcctgcc ctggcaaccg cgcgagagag ccgccggttg
     3001 agttcggcga cgtcggcgaa cggcaggcct gacatcatgc cactgacggg tagggcaagc
     3061 tcgaaggtta ccttttcgtc ttcgacgacg actattcccc cgttgagctc tctgactcgc
     3121 cgggcagccc gagcgataga ctgcgggcgt cttccgagga caagcaggtg ggtggtcgtg
     3181 ttgtaggtac tggcgagtcc gtccaggttc gccgcataac ccgacactag cgcgcgggag
     3241 atccaggctc cttcccgatc tatgagcgcc gcgtgcaacg cgccctctgg ggggacgacc
     3301 agaccgttat cagagcgaac ccgggcgtcc ttgcgctcgg tgatcacggc ggacctaagg
     3361 tgtatgaccg gcacctcagc ctcagatggc ttttgcggat agagtgcggg gtcaccgagg
     3421 tccaggtcag gttcgaaacg cggcctgctc ccgtacgcgt cccactctat gtccgggagc
     3481 gggactcgaa gctctcctct atcggcaaca acgtgccccc gcgtaataac tgtcagcggg
     3541 cgaaaagaca cgaggtcggg cagtagcaag aggtccgccc gacggccgat gcctatacct
     3601 ccgacctggc cgtcgatacc aaacagggtc gcagggttaa tggtaaccat ctgcagcgcc
     3661 tgcatcggcg aaacaccgtt gtcgactgcg acacggagca tgccgtcaac gagcccgtgc
     3721 tctgcgatga actcggggga ggggccgtcg gtggtcatga tcaggcggtg cgtgctcacc
     3781 ccgttctcgg tgacggagcg aaggagctcg ggcagatcgg gacgcaggga gctgttgcgg
     3841 agcatcgtcc agatcccgag ccgcagccgg ttgaggacct cctctctcgt tatggcctcg
     3901 tggtcggcgt ctatgccggc agcggcgaca ggttgcaggc gcgactctga ggcaccgccg
     3961 gtgtggccgt ctacgatctt gccgagggac ttcgccttgc ggatgccgga gatcagggcg
     4021 ggatctcccg ccgccagcgc ggtccagcgc gtgacctctg ccgtaccgag cacgtcgtca
     4081 cgccgcagga ggagctcgac cttttccagc ccgaacatct cctcctctcc gggaaacgcc
     4141 gcctgggagg tgagacgcgc catccacagg tacaggagcg ggaggtcccg caggtcgccg
     4201 gccatcttcg cgaacccttc atggcccatc tgcatgaaga agaagagatt atcggcgacg
     4261 atggcggtgg ttccgagcgg caagacgccc tcgacgaggc taacggggtt gtagcggacc
     4321 cagggatgga agtgcgcctc tatgtatcct ggggcgacga tcattccctt cgcctcgacg
     4381 atctcggtgt cttgaccgac ggtccgctcc ccctcgccga cgaatgctat gcggccagcg
     4441 gtgacggcga cattgccttc atagaattct ccagagtaga cgttggctat cgtgccgcca
     4501 cgcacgaaga ggtccgcagg acgatcaccc cgcgccgtct ccacgagcct cgcgtgttcc
     4561 tctgcgctcg ccagcattga ggctagaccg cctggtagct gaccacgccg tctgccaccc
     4621 gttggacgta gtcctccccc tccaggtgtt cgaggtgggc cagcgtctcg gcgagcgcga
     4681 agcgtagctg gtgatcggtg aggtcttcgg ggaagacacg gcgcgcgacc tcgaatggcg
     4741 tcgccggcct cccgtcgagg gctgcgagca ttaccgagag gcgctcctcg tggtgcgcga
     4801 ggagctcgtc cacgcgccca tccaggtcgt ggaagagcgg accgtgcccc gggaagacga
     4861 ggtccacatc cagtccccgc aggcttgcaa gggagtcgag gtagcgtcga agcggccgcg
     4921 gggccgtgta tgtccacagt ccgatgttcg gcgtgatctc gagcagcagg tggtctccgg
     4981 cgaacaggag cctctgtcgc tcgtcgtgca ggatgaagtg aaaatctgag tgaccaggcg
     5041 tgtgcaccac cctggcctcg ctagctccga gctcgatcga gtctcccggc cgcagcggca
     5101 gcaggcgctc cggcagtcgt acgccgagcc gcgtggatcc tgctgtcggt tcagccgtct
     5161 gcgcgtccat gccgttgcgg atcaagtacc gtacaaagtc ttctgttccc ctgtccgggt
     5221 cccagacgcg gcgggcgttc tcgatctcac cttcgagcat ccagaccggc gccccgctac
     5281 gctcttccag ccagcgggcg agccctatgt gatccgggtg cagatgcgtc acgatgatcc
     5341 gtgccaggtc acgctccagg tccaggccaa cctttgctgc gcctctctcc cacgtctcgc
     5401 gggctggcgg atagtcgaag ccagggtcta tgaccgtcca cccgtcgcag tcctcgacga
     5461 ggtacgacgc gacgaacttc agggggaaag gcacaggcaa cttgacctga aaaatgtcgt
     5521 ccgagacacg cgaggtctcg ccgatgggct gcgtgccaag agaagaagga gcgacgctca
     5581 cgagcacgcc tctaagcgcc ggcggtcaga tatgtcagca cggagcgttt gcatatctca
     5641 cggtactccg cgtgggtctt ctcccagtcc ttctcctgga tccactgcgt gaaaagcccg
     5701 tccacgaggg cccgcaccac cgtcgcgcct tctcgagagt cgcctccgag gaagacaccc
     5761 tcctcttgac cgagctcgat gacctcggcg tagaggccgt tgcagatctc atgaaacgtg
     5821 gctcccacgt cagcgaagcg gtcgttgcgg gccgcgtagc caaggaagtc gaagaagacg
     5881 aggtagaacc tgcggtttga ctcagggctc acgaagatgg cgtcgatcat agccgagacc
     5941 ttctcctccg cggagggcgc ctcggcggtc gcctcgcgga tgcgtccggc gacgcgggcg
     6001 agcacccagc gcattgtgag gaggatcagg ttctccttgg actcgaagta gtagggtagg
     6061 atggccttgc tcaccctggc ctcgtccgcc acgtcctgca gtgagaggtg gcgcaacccc
     6121 ttctcgccca tgaccttgta ggcgctgcgt atcaggcgtg cctgcttcgg cgccagcgcc
     6181 tcgccttcgc ggtctgacga gcggatcagg gtgtcggatg tggccacgat gcactcacct
     6241 ccatgtgccg gattgtacct taggggagca aagtgttaca gcctacttac taaactccgg
     6301 ctttcgcttc tccatgaacg cggagatgcc ttccctggcg tcctcgcttt cgaaaagcgg
     6361 ctcgacgagc gtacgctcgg tctcgaggcc ttcgtccagc ggcctttcga taccgccgta
     6421 taccgcgagc ttgatggacc cgatggcctc ggtagcgccg cccgccagtt tctctgcgta
     6481 cttgcgagtc tcctcttcca gagcgtcggc agggaacagc cgatcgagga tgccgacctc
     6541 gtgggcctca gaaggtgaga tcgtccgtcc cgtcaccatg agatcgaggg ctttgctcac
     6601 cccgataagg cgcggcaggc gctgggtacc gccgttgccg gggagcagac cgagggtcgc
     6661 ctcaggcagc ccgagcacgt actcgccctc agcaccgaag cgaaggtcgc aggccagggc
     6721 caactccagt ccacctccga gggcggtcgc cccgatctgg gcgatgaaca ctttcggggt
     6781 gtgtgctatc tcggagaggc cttcgtgggc ctgactgatc atgcgcatat tatcctcgac
     6841 gctgttggcg tcaaacgcct tgatgtccgc ccctgcggag aagaacctct cgctgccgct
     6901 gcgcaggatc acggctccgg tcccttcgtc ctctgccgcg gagcgcacgc actcgcttag
     6961 ctcctccata aacgagaggt catagctgtt cgccggcggg ttgtccaggg tgatgtaccc
     7021 tacgctgcct tctctcgtat actcgacggc catttatcct cctctaacgt ctctaacgga
     7081 tagccgtagg catagggccg cggacactac agaggtccgc gacccgatac cccactcagt
     7141 caagtcctcc gacgactact gacttctgct ccaggtagtg ttcgatggcc tccttgccgt
     7201 gctcgcggcc tatgcccgag tctttaaccc ctccgaacgg aagctcgtcg tagccgaagt
     7261 ggagctggtt cacccaggtc atgcctgcct ctatctcctg ggcggcacgg tggatccact
     7321 tcacgttgta cgtgtagacg gaggagccta ggccataggc agagtcgttg gctagtctaa
     7381 tcgcctcgtc cacatcattt accttgaaga ccggcagcag tggcccgaag gtctcctcgc
     7441 gcaccactcg gctgtcgtgc ggggcgtcct cgatcacggt cggctcgaag aagtagcctt
     7501 tgccctcgtg cccattaccc ccgacggcga ccttcgcgcc cccgtccacc gcgtcctgga
     7561 gttgatctgc cagcacgtca cggtgcttgg cggcgttgag gggacccatc cttatcttgg
     7621 gcttctccgc tttctccatc ccgtcg
//
LOCUS       DASTKM010000263         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_270187, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000263 DASTKM010000000
VERSION     DASTKM010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270187"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VFQ10_10660"
     CDS             complement(<1..883)
                     /locus_tag="VFQ10_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9965845.1"
                     /translation="METVIQGSTLQTLRAGLRGTAHAPGEEGYEEASRAWNLAAHQTP
                     ALVVVAKGAADVLAAVRFAREGGLGVGVMATGHGVGTACDGGVLINTSGMRGVRVDPV
                     DKTARVEAGALWLDLIHESQPHGLAGLLGSTSYVGIVGYTMGGGFGWLGRKYGFNAAS
                     VREADVVTADGELVRVSAEEHPDLFWGIGGGGGNFGIVTSLKFDLYPIGMLYGGNLIY
                     PVEKAPEVLEAYARWSADLPDEMTSGVAFLNVPPLPALPEPLRGKSVIALRGCYCGES
                     PGDGEELVRPMREELGEP"
     gene            complement(1010..1978)
                     /gene="corA"
                     /locus_tag="VFQ10_10665"
     CDS             complement(1010..1978)
                     /gene="corA"
                     /locus_tag="VFQ10_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET9965846.1"
                     /translation="MIVDNAIYVDGHRTAEPVTLEETYEAVRNRRGVAWIGLYKPTVE
                     EFSSVAGEFGLHGLAVEDATDAHQRPKLERYGDTLFVVLKPARYLDEEERVEFGEVHL
                     FVGKDFVVSVRHGESPDLGGVRKQLEADPEHLRRGPEAILHAIMDRVVDDYGPVVDGL
                     ENDIEEIETEVFGGNPNVSRRIYELSREVIEFQRATEPLGGVLGDMMRDETVDVDPEV
                     RRHLRDVRDHVLQVTERLAGFRELLQSILSVNLTLSSLAQNEQVKKISAWAAILFAPT
                     LIGTIYGMNFDYMPELGWPLGYPFALVLMGFTSLTLYFIFKRRGWM"
     gene            complement(2494..2865)
                     /locus_tag="VFQ10_10670"
     CDS             complement(2494..2865)
                     /locus_tag="VFQ10_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HET9965847.1"
                     /translation="MDRTTHSPRITRLSWGRLETESAVFKDVRLYPGGAEEWDWNETG
                     TSHDPGIQPADVERLLERGSTVVILSRGFHERLGVAPETLRMLEEREIPAHVVQTEEA
                     ARLYEELRKTEKVGALIHSTC"
     gene            complement(2897..3694)
                     /locus_tag="VFQ10_10675"
     CDS             complement(2897..3694)
                     /locus_tag="VFQ10_10675"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET9965848.1"
                     /translation="MSESGQQRGESPLLSDRGATTISDTVVSSIAGMAAQEVEGVHMG
                     GGASRTAGGVLGSLTGSESKTSGISVEVGRTETAIDLKMGIEYNKNILQTVEEVRRRI
                     TDRVQSMTGLRIRELNATITDITFPEKEQRRRIGSGSATRERDASADETPTAPAQATM
                     PVSEPDPEASERETRQVETRSQAETTPEEEASVEGRQPEEAETADLRSGDTRTEEQPA
                     GEALETSEEAETRAETETSEPTETSAEARRRRRAEERARRRRRTEDS"
BASE COUNT          591 a         1303 c         1082 g          741 t
ORIGIN      
        1 tcggctcgcc gagctcctcg cgcatgggac gcaccagttc ctcgccgtca ccaggcgact
       61 ccccgcagta acagccacgc agcgctatta cagacttgcc gcgtagcggc tcggggagcg
      121 caggcagagg agggacattc aggaacgcca ctcctgaggt catctcgtcg gggagatcag
      181 cgctccagcg ggcgtaggcc tccagcacct cgggcgcctt ctccacaggg tagatcaggt
      241 tgccaccgta gagcatcccg atggggtaga ggtcgaactt cagggaggtg actataccga
      301 agttgccacc tccgccccca ataccccaga acaggtcggg atgctcctcg gcgctcacgc
      361 gcaccagctc gccatccgcg gtgactacgt ctgcctccct cacgctcgcg gcgttgaaac
      421 catacttgcg cccgagccac ccgaagccgc cgcccatggt gtagcccacg atgccgacat
      481 atgaggtgga acccaggagg ccggccagac cgtgcggctg ggactcgtgg atcaggtcga
      541 gccacagcgc gccggcctcg acccgcgccg tcttgtctac agggtccacc ctgacccctc
      601 tcatgcctga ggtgttgatc aggaccccgc cgtcgcaggc agtgcccacg ccgtggcccg
      661 tcgccatcac accgacgccc aacccccctt cgcgggcgaa acgcaccgct gccagcacgt
      721 cggccgcgcc cttggccacg acgaccagcg caggagtctg atgggctgcc aggttccacg
      781 cccggctcgc ctcctcgtaa ccttcctctc caggcgcgtg tgcggtgccg cgcagaccgg
      841 ccctcagggt ctgtagcgtc gagccctgga tcaccgtctc cataatcgtc tcccttccct
      901 ctcgaggttg cgagccggca ggtcaattcc tcccggccgg ttcagataac agcacccagc
      961 ctaacgtccg agcatagaag gcgcatcccc caaatggagt attttcgacc tacatccagc
     1021 cgcgacgctt gaagatgaag tagagcgtga gagaggtgaa acccatgagc accagggcga
     1081 aggggtagcc gagcggccag cccaactcgg gcatgtagtc gaagttcatc ccgtagatcg
     1141 tccctatgag agtgggcgcg aacaggatgg cggcccaggc ggagatcttc tttacctgct
     1201 cgttctgggc caggctcgac agggtcagat tcacgctgag gatgctctgc agcagctcgc
     1261 ggaagccggc gagtcgttcc gtaacctgca ggacatggtc acggacgtcg cgtagatgcc
     1321 tccgtacctc cgggtcgacg tctacggtct cgtcccgcat catatctcca agcacaccgc
     1381 cgagcggttc cgtcgccctc tggaactcga tcacctcgcg cgagagctcg tagatcctgc
     1441 gcgacacgtt gggattaccc ccgaagacct cggtctctat ctcttcgata tcgttctcga
     1501 ggccgtcgac tacgggcccg tagtcatcaa cgacacgatc catgatggcg tgcaggatcg
     1561 cctcgggtcc acggcgaagg tgctcggggt cggcctccag ttgcttgcgt accccgccca
     1621 ggtccggcga ctcgccgtgg cgcacgctca ctacgaagtc ttttcccaca aagaggtgga
     1681 cttcgccgaa ctcgaccctc tcttcctcgt ccaggtagcg ggccggcttg agcacgacaa
     1741 agagcgtatc cccgtagcgc tccagcttgg gcctctggtg ggcgtccgtc gcatcctcga
     1801 ccgccagacc gtgcaggccg aactccccgg cgacggacga gaactcctct accgtcggct
     1861 tgtagaggcc gatccatgca accccgcgcc ggtttcggac ggcctcatag gtctcctcga
     1921 gggttaccgg ctcggcggta cggtggccgt ccacgtagat ggcattgtcc acgatcacag
     1981 ccgcattata cgcccgcgcc tgtttctgct ggtatgcttc agcccacggc gacgcgcttg
     2041 tgtcctctac tcgtcggtct ccccctcgaa gacggcgaac gcggcaccct ggggatcgcc
     2101 aaggacggcg atcttgcccg aagggagatc caagggccca gccagcacgg taccgcagag
     2161 ctcgcgcgcc ttctcgacgg cggcgtcgcg cgatcagacc ccccattccc aaccagcaaa
     2221 cgtccaagat ggagcctggc tgtccgatat tggatacgac cccaatggcc ttgacgggat
     2281 gtgccttctc tccgccagaa ggacaccaag tgatgcttca ggcgtacttc cagaaaacca
     2341 gtcgtccgag gtgcgggctc ttcggaaggg cactctcccg acttatcgac agggctacct
     2401 tctgcctgtt ctgccccgcc gccggcacag tggcagaggc ttcgacaact cgcatctctt
     2461 gctctacttt ctctaggtca agcaacactg tggtcagcag gtcgagtgga tgagcgctcc
     2521 caccttctca gtcttccgca actcttcgta gaggcgggca gcctcttctg tctgtacgac
     2581 gtgcgcgggg atctctcttt cttccagcat cctgagggtc tcgggcgcga cgccgagacg
     2641 ttcgtggaag cctctggaga ggataacgac cgtcgagccg cgttccagca gtctctctac
     2701 gtcggccggc tgtatgccgg gatcgtggct tgtacccgtc tcgttccagt cccactcttc
     2761 cgcgcctccg gggtagagcc ttacgtcctt gaagacggcg ctttcggtct ccaggcggcc
     2821 ccaggagagc cgggttatcc gcggagagtg tgttgttcga tccatcctgc tcaccctcca
     2881 agagatccgt gggagtctaa ctgtcctcgg tacgcctgcg ccgcctggcg cgctcctccg
     2941 cgcggcgcct gcgtcgagcc tccgcgctcg tttccgtggg ctcggaggtc tcggtctctg
     3001 cgcgtgtctc cgcctcctca gacgtttcca gcgcttcgcc cgccggctgc tcctctgtcc
     3061 tggtgtcgcc agaacgcagg tctgcggttt cggcttcctc gggttggcgg ccttccacgc
     3121 tggcctcttc ctcaggagta gtctcggcct ggctgcgcgt ctctacttgc ctcgtctcgc
     3181 gctcgctggc ttcgggatcg ggttcgctca caggcatagt tgcctgcgcc ggagcggtag
     3241 gcgtttcgtc cgcggaggcg tcgcgctccc tcgttgcgct ccccgacccg atgcggcgtc
     3301 tctgttcctt ctcggggaag gtgatatccg taattgtggc gttgagctcc ctgatccgga
     3361 gccccgtcat gctctggacg cggtccgtga tcctgcgccg cacctcctcg acggtctgca
     3421 ggatgttctt gttgtattcg atccccatct tgagatctat ggctgtctcc gtccgcccga
     3481 cttcgacgga gatcccgctg gtcttgctct ccgaacccgt caggctcccc agtaccccgc
     3541 cggcggtcct cgaggcgcct ccgcccatgt ggacgccctc cacctcttgc gccgccatgc
     3601 cggctataga ggagactaca gtgtcactga ttgtcgtggc cccgcgatcg ctcaggagcg
     3661 ggctttcacc acgctgctgc cctgactcgc tcatctctca ctctcctcct tcgacca
//
LOCUS       DASTKM010000264         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_270828, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000264 DASTKM010000000
VERSION     DASTKM010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270828"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1305)
                     /gene="fdhA"
                     /locus_tag="VFQ10_10680"
     CDS             complement(79..1305)
                     /gene="fdhA"
                     /locus_tag="VFQ10_10680"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR02819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HET9965849.1"
                     /translation="MADNRVVVYKGPGEVEVEDIEYPKLEVPEEVASAMGMTKEAPHA
                     VILKIISTNICGSDQHMVRGRTTAPVGQTLGHEITGEVIEKGNDVQFIDIGDLCSVPF
                     NIACGRCRNCKERKTGVCLNVNPARAGSAYGYVDMGGWVGGQAMYVMVPFADFNLLKF
                     PDKEQAMEKILDLTMLSDIFPTGYHGCYTAGVTQGSTVYVAGAGPVGLAAAHSAQILG
                     ASVVIVGDLKNERLAQARHFGCETVNVSEDAELHDQIAEILGVPEVDCAVDAVGFEAS
                     AHGPDAIEAPATVLNAVMSITRAGGAVGVPGLYVTGDPGAPDPKAQEGTLEIRIGLGW
                     AKSHALYTGQCPVMKYNRDLMMAILHDRAQIARAVNATVISLDEAPQGYKDFDKGTAK
                     KFVLDPHGDLQGAERV"
     gene            1623..>3253
                     /locus_tag="VFQ10_10685"
     CDS             1623..>3253
                     /locus_tag="VFQ10_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2187..2189,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9965850.1"
                     /translation="MLLRLAEKDGLWKKIRNHVGTTVEHPVSPATRAIRSRISDESVE
                     KTVSVCPYCAVGCSTLVYHRDGRIMDIEGNPDSPINAGTLCPKGSATFGLHVSPYRWT
                     KVRYRRPFSGEWEDLSLEEALDMIAERVKMTREETWEAKDAEGKKLNRSMALASIGGA
                     TIDNEENYLITKLFHSLGFVRITNQARIUHSSTVPGLGITYGRGGATTNLQDLQNADC
                     ILIEGSNMAEAHPVGFRHPMIAKKKGAKLIHVDPRFTRTSAMCDIYAPIRPGTDIAFL
                     GGLINYVLQNEEYFEEYVKIYTNAATIITDDFEDADASGLFSGWDEESKQYDPTSWRY
                     EGHPIDYASVGGSQGRIVGEAGEGGGGEMHPGMPRPTDMSLEHPRCVFQILKRHFARY
                     TPEMVEKVCGTPKEAFLEVARTLAENSGREKTSALCYAVGWTQHSKGVQIIRTAAILQ
                     LLLGNIGRPGGGIMALRGHATIQGSTDIATLYDILPGYLPMPDANKDHDTWQSYLQNE
                     TARSGWWSNFPKYVISQMKAWYGEKFDPESGDLFD"
BASE COUNT          623 a         1039 c          996 g          595 t
ORIGIN      
        1 ccggcctgtt gattatgttc ggtcacgtca taagagaaga ggggcggata gtattccgcc
       61 cctcttgtgc gaccgctctc agactctctc ggcgccctga aggtcgccgt gtgggtcgag
      121 cacgaacttc ttggccgtcc ccttgtcgaa gtccttgtag ccttgcggag cctcgtcgag
      181 cgagatgacc gtcgcgttga cggccctggc gatctgggcc ctgtcgtgga ggatcgccat
      241 catcaggtcc ctgttgtact tcatgaccgg gcactggccg gtgtagaggg cgtggctctt
      301 ggcccagccg agcccgatgc ggatctcgag cgtcccctcc tgggccttgg ggtccggggc
      361 gccagggtcg ccggtgacgt agaggccagg gacgcccaca gcgccgccgg cgcgcgtgat
      421 gctcatgacg gcgttgagga ccgtcgccgg agcctctatt gcgtcaggtc cgtgcgcgct
      481 cgcctcgaag cctacggcgt ctaccgcgca gtcgacctcg gggacgccga ggatctcggc
      541 gatctggtcg tgcagctccg cgtcctctga gacgtttacg gtctcgcagc cgaagtgacg
      601 cgcctgcgcc agacgctcgt ttttcaggtc gcccacgatc acgaccgaag ccccgaggat
      661 ctgtgccgag tgagccgctg cgagccctac cgggcctgca cccgctacgt agaccgtcga
      721 tccctgggtc acgcccgccg tgtagcagcc gtggtagccg gtggggaaga tgtccgagag
      781 catcgtgagg tccaggatct tctccatcgc ctgctctttg tccgggaact tgagcaggtt
      841 gaagtccgcg aaggggacca tgacgtacat ggcctgcccg ccgacccagc cgcccatgtc
      901 cacgtagccg taagccgagc ctgccctcgc cgggttgacg ttgaggcaga cgccagtctt
      961 gcgctccttg cagttcctac agcgtccgca ggcgatgttg aaaggtaccg agcagaggtc
     1021 gccgatgtct ataaactgga catcgttacc cttctcgatg acctcgcccg tgatctcatg
     1081 cccgagcgtc tgccctacag gcgccgtcgt ccttccgcgg accatatgct gatcggagcc
     1141 gcagatgttg gtcgagataa tcttgagtat cacggcgtgc ggagcctctt tggtcatccc
     1201 catcgcgctc gcgacctctt cggggacctc gagcttcgga tactcgatgt cctcgacctc
     1261 tacctcccct ggtcccttgt agaccactac cctgttgtca gccattagct aaatctctcc
     1321 tttccctcta gccggagacg atcagaagca acaattcata ttcccgctcg accgcgccgc
     1381 caaacttacc tctgccggcg cgggggcgca tctcagatat ccaagcaagc tcctttcccc
     1441 ccgaaactca ctcctcgcaa agtaccagat gttgcgtgcc gatgcaagaa caagactaca
     1501 tataagggtg ggcagcgcag ctgcgtgacg gattgggtcg tggtttcgcg agggggtgaa
     1561 tagagttaga atcaggaggg gagactcgga cattcacatt cttggcatag aaggggtcag
     1621 gtatgctctt aaggctcgcg gagaaagacg gtctctggaa gaagatccgg aaccatgtcg
     1681 gcacgacggt cgagcacccc gtaagcccgg cgacccgcgc catccgctcg cgcatctccg
     1741 atgaaagcgt agagaagaca gttagcgtct gtccgtactg tgcggtcggc tgctcgacgc
     1801 tcgtctacca ccgcgacggg cgcattatgg acatcgaggg gaaccctgac agccccataa
     1861 acgctggcac gctctgtccg aagggctcgg ccaccttcgg gctgcacgtc tcaccttacc
     1921 gctggaccaa ggtccgctac cgcaggccgt tctctggcga gtgggaagac ctctcgctcg
     1981 aggaggcgct cgatatgatc gccgagaggg tcaagatgac cagggaggag acctgggaag
     2041 cgaaagacgc ggagggcaag aagctgaacc gctccatggc cctggcctcc atcgggggcg
     2101 ccacgatcga caatgaggag aactacctca taaccaagct cttccactct ttggggttcg
     2161 tgcggatcac gaaccaggcg cggatatgac acagctccac ggtgcccggt ctgggcatca
     2221 cgtacggtag gggcggcgcc accaccaacc tgcaggatct gcagaacgct gactgcatcc
     2281 tcatcgaggg ctccaacatg gcagaggccc acccggttgg cttcaggcac cccatgatcg
     2341 ccaaaaagaa gggcgccaag ctcatccacg tcgacccgcg cttcacgcgc acctcggcga
     2401 tgtgtgacat ctacgcaccc atcaggcccg gcaccgacat cgctttcctg gggggcctga
     2461 tcaactacgt cctccagaac gaggagtact tcgaggagta cgtcaagatc tacacaaatg
     2521 cggccacgat catcaccgac gacttcgagg acgccgacgc ctcgggcctg ttctccggct
     2581 gggacgagga gagcaaacag tacgacccta cgagctggcg ttacgaggga cacccgatcg
     2641 attacgcttc cgtcgggggg agccagggac gcatcgttgg cgaggccggc gagggcgggg
     2701 gcggggagat gcatcccggc atgccgcgcc cgaccgacat gtcgctggag cacccgcgct
     2761 gcgtgttcca gatcctcaag cgccacttcg ccaggtacac gcccgagatg gtcgagaagg
     2821 tctgcggcac cccgaaggag gctttcctcg aggtagccag gaccctggcc gagaactcgg
     2881 gacgggagaa gacctcggcg ctgtgctacg cggtcggctg gacgcagcat tccaagggcg
     2941 tgcagatcat ccgtacggcc gcgatcctac agctcctcct gggcaacatc gggcgtccgg
     3001 gcggcggcat catggcgctg cgcgggcacg cgaccatcca gggctcgacg gacatagcca
     3061 ctctctacga catcctccct ggctatttgc cgatgcctga cgcgaacaaa gaccacgata
     3121 cgtggcagag ctacctccag aacgagactg ccaggagcgg ctggtggtct aactttccca
     3181 agtacgtaat cagccagatg aaggcctggt acggcgagaa gttcgacccc gagagcggtg
     3241 atctcttcga cca
//
LOCUS       DASTKM010000265         3849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1502510, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000265 DASTKM010000000
VERSION     DASTKM010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502510"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFQ10_10690"
     CDS             complement(<1..454)
                     /locus_tag="VFQ10_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9965851.1"
                     /translation="MSSRIPQDIERQAFDLIVVGAGINGTGVARDAAMRGLRVLLLDK
                     GDISSGTTQWATRLIHGGLRYLEHYEFALVRESLRDREAMLHIVQHLVKPLGFLVPIY
                     DRSSRGPLMIRLGMNVYDVLSYDRSMDRHKMLSRKKVQEREPGLNPEGL"
     gene            complement(457..1539)
                     /locus_tag="VFQ10_10695"
     CDS             complement(457..1539)
                     /locus_tag="VFQ10_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965852.1"
                     /translation="MTTVSVLGSGIMATALAFPLSDNGHEVRLIGTHLDREIIDSIKE
                     TGVHPGLDLEVPRAARAFQLEEAEEAFDGADVVMSGVNSFGVRWAGGQLSSLLQPGMH
                     VLSIAKGMEATESGDLRILPEVLAGEVPEGLREQVSWSAIAGPSIAGEVAVGRDTCVV
                     FTGRDAGVLEEMAGLFRTPYYHVWTSSDFIGVEVCAAAKNCYALGAGFMEGILDREGQ
                     SEARYRNYDYGAALFGQATRELRRFMELLGGEPETPYGLAGVGDMFVTSMGGRNVKVG
                     RLVGSGLRFSEARERMPGVTLEGAAAIEVIGGALPKLTDRGIIRPEDFPLMRHLHSVV
                     GLDEPLDMPWRTFFGGEPPQSKAGKG"
     gene            complement(1536..2960)
                     /locus_tag="VFQ10_10700"
     CDS             complement(1536..2960)
                     /locus_tag="VFQ10_10700"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HET9965853.1"
                     /translation="MGAKLIEAALQEATDTQNVNIGGRALASLPGVFREGFGDNPAVV
                     VADGNTFEVAGKEVRRRLERAGHTVMEPYVFPAEPPLYTEYSNIVTLRDSLLEHDAIP
                     VAVGSGTINDIVKRSAHECERSYMCVATAASMDGYTAFGASIEKDGLKQTLSCSAPRA
                     VVADLEVLKHAPGDMTASGYADLLGKVTSGADWLVADALQVEEIDPTGWSLVQDHLRG
                     WTDSPAELRAGDLKSTEQLMEGLILSGLGMQSYQSSRTASGAEHQFSHLWEMEGLGHG
                     EGHRGTPLSHGFKVGVGTVAIAALYERVLERDLGDLDIEALHASWPTRDAMERSVRAQ
                     HTVPDLERGAVEQTLAKYISAGKLAQRLELLRDVWPGLRQKVRVQLMPAKQIREKLQA
                     AGCPTGPVEIGLSWEDFEATYLRARTIRKRYTVLDLAFEAGILEECVDELFAPEGYWG
                     RAAASHARIDGTRVAGSFQRSEEA"
     gene            complement(2966..>3849)
                     /locus_tag="VFQ10_10705"
     CDS             complement(2966..>3849)
                     /locus_tag="VFQ10_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET9965854.1"
                     /translation="ELGLKAGTPVAEGGVDAYVGAIGLGVVEPGKMALITGSSHVMIG
                     QSARPIHDPGFWGAYTDAMIPGQYSVEAGQASTGSIVAWFKNQFAGDAAAEARERGVD
                     TYDVLTEQARDVPIGSDGLIVLDYFQGNRSPYTDPLARGMMWGLTLSHTPGHVFRAII
                     EGICYGTENILRTMRGQDFEPKLNVVSGGPAKSELWMQMHADVSNVPISFTRVSEGPV
                     LGSAMLAAVGAGIYPDIPAAAENMVHTADTIEPDPGRHEEYRFYVDRYMEAYPQMKEL
                     MHKTERHVASGTPAAGE"
BASE COUNT          652 a         1310 c         1154 g          733 t
ORIGIN      
        1 tcaggccttc cgggttgagg cccggctcgc gctcctggac cttcttgcgg ctgagcatct
       61 tgtgcctgtc catgctcctg tcgtaggaga gtacgtcgta gacgttcatg ccgagcctta
      121 tcatgagcgg cccgcgggag ctcctgtcat agatggggac gaggaagccg agcggcttga
      181 ccagatgctg cacgatgtgc aacatcgcct ctctatccct caacgactct cttacgagcg
      241 cgaactcata gtgctccagg tagcgcaagc cgccgtggat cagacgcgtc gcccactgcg
      301 tagtcccgct ggagatatcg cccttgtcca ggagcaacac ccgcaagccc cgcatcgcgg
      361 cgtcccgggc tacgccggtc ccgtttatgc cggctccgac cacgatcaga tcgaaggcct
      421 gcctctcgat gtcttgcggt atgcgtgatg acatctctat cctttccctg ctttactttg
      481 tggtggttcg cccccgaaaa acgtacgcca gggcatgtcc agaggctcgt ccaatccgac
      541 cacggagtgc aggtggcgca tcagggggaa gtcttcgggt cgaatgatac cgcggtcggt
      601 aagcttggga agcgcgccac cgattacctc gatagccgcc gccccttcga gcgtcacccc
      661 aggcatccgc tcgcgcgcct cggagaaccg cagtcccgag cctacgagac gccccacttt
      721 cacgttacgt cctcccatgc tggtgacgaa catatcccca acccctgcca atccgtatgg
      781 tgtctcgggc tcgccgccga gcagttccat gaaccgcctc agctcccgcg tcgcctggcc
      841 gaagagcgcg gccccgtagt catagttacg gtaccgtgcc tccgactggc cctcacggtc
      901 gagtatgccc tccataaaac cggcaccgag agcgtagcag ttcttggccg cagcgcacac
      961 ctcgacgccg atgaagtcgg acgaggtcca cacatgatag tagggtgttc ggaagaggcc
     1021 agccatctcc tccaacacgc cggcgtcgcg gccggtgaag accacgcacg tatcccgccc
     1081 cacggcgacc tcgccggcga tggagggtcc ggctatggcc gaccaggaca cctgctcccg
     1141 aaggccctca ggcacctccc ctgcgagcac ctcgggcagg atgcgcaggt cgccgctctc
     1201 cgtggcctcc atccccttgg cgatggagag gacgtgcatg ccaggctgga gcagcgaaga
     1261 gagctggccg cctgcccagc gcaccccgaa cgagttgacc ccgctcatga cgacatcggc
     1321 cccatcgaac gcctcctcgg cctcctcgag ctggaaggct ctggccgccc gggggacctc
     1381 gaggtcgagg ccaggatgca cccccgtctc cttgatgctg tcgatgattt cacggtcgag
     1441 gtgcgtgccg atcaggcgta cctcgtggcc gttgtccgag agcggaaaag cgagggccgt
     1501 ggccattatg cccgagccaa gaacactaac ggtcgtcatg cttcttccga acgttggaac
     1561 gagcctgcca cccgcgttcc gtcaatcctt gcgtgcgagg ctgcggcacg gccccagtag
     1621 ccctcaggcg cgaacagttc gtccacgcac tcctccagga tgccagcctc gaaggccagg
     1681 tcgaggacgg tatagcgctt gcggatcgtc cgcgcccgta agtacgtcgc ctcgaagtcc
     1741 tcccagctga gcccgatctc gacggggccg gtgggacaac cggcggcctg tagtttctct
     1801 cttatctgtt tggccggcat cagctgcact ctaaccttct ggcgcagtcc tggccacacg
     1861 tcccgaagca attccagcct ctgcgcgagt ttgcccgcgc tgatgtattt ggcgagggtc
     1921 tgctcgacgg cgcctctctc caggtccggg accgtatgct gcgcccgcac ggatcgttcc
     1981 atcgcatccc tggtgggcca ggaggcatgg agcgcctcga tgtctaggtc gcccaggtcg
     2041 cgttccagta ctcgttcgta gagcgcggcg atggccaccg tgccaacccc taccttgaag
     2101 ccgtgggaga ggggagttcc tctgtgacct tctccgtgtc ccaggccttc catctcccac
     2161 aggtggctga actggtgctc ggctcccgag gccgttcttg aagactggta ggactgcatc
     2221 ccgaggccgg acaggatcag accctccata agctgctcgg tggactttag atctccggcg
     2281 cgcaactcgg ccgggctgtc cgtccacccg cgcaggtggt cctgcaccag cgaccagcct
     2341 gtaggatcga tctcctcgac ctggagggcg tcggcgacca accagtcggc acctgatgtc
     2401 accttgccga ggagatccgc ataccccgag gccgtcatgt ccccgggagc atgcttcagg
     2461 acttccaggt cagccaccac agccctggga gcggaacagg agagcgtctg cttcaacccg
     2521 tccttctcga tcgaagcgcc aaaagcagtg tacccgtcca tcgatgcggc tgttgcgacg
     2581 cacatatagg acctctcgca ctcgtgggcg gaccgcttga cgatatcgtt tatagtgccc
     2641 gaccctacgg ccacagggat cgcgtcgtgc tccagcaagg agtccctcaa cgtcacgatg
     2701 ttcgagtact cggtgtatag gggcggctcc gccgggaata cataaggctc catcacagtg
     2761 tgcccggctc tttcgagccg ccgcctgacc tccttgcctg ccacctcgaa cgtgttcccg
     2821 tcggcgacta cgaccgcagg gttgtcgccg aaaccctccc tgaagactcc ggggagagag
     2881 gcgagcgcgc gccccccgat gttcacgttc tgtgtgtccg tagcctcctg cagggccgct
     2941 tcgatgagct ttgcgcccat ataatctact cccctgccgc gggcgttccg ctggcgacgt
     3001 ggcgctccgt cttgtgcatc agctccttca tctgcggata tgcttccatg tagcggtcca
     3061 cgtagaacct gtactcttcg tggcgcccgg ggtcgggttc gatggtgtcg gcggtgtgga
     3121 ccatgttctc ggccgcggcc gggatgtcgg ggtagatgcc ggccccgacg gcagccagca
     3181 tcgccgagcc gagaacgggg ccctcgctca ctctggtgaa cgagatcggc acgttcgaca
     3241 cgtcggcgtg catctgcatc cacaactcgc tcttggccgg tccaccggag acgacgttga
     3301 gcttcggctc gaagtcctgt ccgcgcatgg tgcgcaggat gttctccgta ccgtagcaga
     3361 tgccctcgat gatggcgcgg aagacgtgtc caggagtgtg gctcagggtc agtccccaca
     3421 tcatgccgcg agccaggggg tcggtatagg gagacctgtt gccctggaag taatcgagta
     3481 cgatcagccc atccgaaccg atgggcacgt cgcgcgcctg ctcggtcagg acatcgtaag
     3541 tatccacgcc gcgctcacgg gcctcggcgg ccgcgtcgcc cgcgaactgg ttcttgaacc
     3601 aggccacgat ggagcccgtg gaggcctgcc cggcctccac gctgtactgg cctgggatca
     3661 tagcgtccgt gtaggcgccc cagaaacctg gatcgtggat aggtctcgca ctctgcccga
     3721 tcatgacgtg cgaagagcct gtgatcaggg ccatcttccc cggctcgacc accccgaggc
     3781 cgatcgcccc gacgtaggcg tctactcctc cctcggcaac gggagtgcca gctttcaggc
     3841 ctagttcct
//
LOCUS       DASTKM010000266        17738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_93076, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000266 DASTKM010000000
VERSION     DASTKM010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 17738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17738
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93076"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFQ10_10710"
     CDS             <1..286
                     /locus_tag="VFQ10_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HET9965855.1"
                     /translation="MMLSHILLRRNEPDLERPYRTPGGMATSGVALVLAVLAVIATFI
                     VDPTAAFITLGVYVLFLAYFGFYSRHHLVAQAPEEEFATIEKAESELAGS"
     gene            300..1664
                     /locus_tag="VFQ10_10715"
     CDS             300..1664
                     /locus_tag="VFQ10_10715"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET9965856.1"
                     /translation="MDTIQAAGRLDLDTLRSEVEAGTIDTVLLAMTDMQGRLQGKRLT
                     ATHFLDEVVEHDAEGCNYLLAVDVEMNTVEGYAMSSWERGYGDFVFKPDLDTLRRVPW
                     QEGTALVTCDLLWEDGSPVVASPRQVLKRQLGRLSERGLEAFVGTELEFIVFMDSYEE
                     AWRKGYRDLEPANYYNVDYSLLGTARIEPLLRRIRNSMAGAGLAVEDAKGECNFGQHE
                     INFRFDRALTTADGHAIYKNGAKEIAAQEGCSISFMPKFDEREGNSCHIHLSLRAEDG
                     APVFAEDHGFSDTFNHFLAGQLASLRELTLFFAPNINSYKRYAKGSFAPTAVAWGKDN
                     RTCSMRVVGHGGSLRVENRLPGGDVNPYLAIAAMIAAGLHGIENELSLEDEFEGNAYE
                     ADKPHVPTNLREAQELFAQSETARSAFGDEVVEHYLNMARVEIEAFEAAVTDWERYRN
                     FERL"
     gene            1661..2383
                     /locus_tag="VFQ10_10720"
     CDS             1661..2383
                     /locus_tag="VFQ10_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET9965857.1"
                     /translation="MKGKSRPNIGVTAATEQINSGVWEEIPAVISPARYVEAVQRAGA
                     RAVLLPPDPEDAEDPGGVLDLLDALVVTGGAGDLDPALYGDERHPETGPVQEERDAYE
                     LSLVRGALEREMPVLGICRGMQVLNVAYGGTIEQHLPDVLGHEEHRRTPGTFADHEVR
                     LVPDSLAARAAGSERTPAKSHHHQGIKAVGEDLQVTGWAVEDDAVEALEDPSCPFVLG
                     VLWHPEEDEKSQLIKALVSEVS"
     gene            2384..3745
                     /locus_tag="VFQ10_10725"
     CDS             2384..3745
                     /locus_tag="VFQ10_10725"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9965858.1"
                     /translation="MLEVLNPATEEVVESLEPATEDDADAAIARARDAFPAWRDVQPG
                     DRSRLLRRLADALDAEQENLAQLESKNTGKPIGDSRGEMAMVAETFHYYAGMPERLLG
                     DTIPVSGGVDVTFREPLGVVGLIVPWNFPLTIASWKMAPALAAGNTLVLKPAELTPLS
                     AMEFERIALEAGIPEGVVNVVVGPGSKVGKRLVEHPDVAKVAFTGSTEVGKGIMAGAA
                     GTIKRVTLELGGKSANVVFADADLQKAAASAPIAVFGNAGQDCCARSRILVEKGAFDE
                     FLSLMQPVVEGFRVGDPLDEETEMGPLISADHRERVAAYVPEDAPVAIRGEAPEGSGF
                     WFPPTVLAPLSNGDRAAREEIFGPVVAVIPFEDEEDAVRQANDTVYGLSGSIWTENGA
                     RALRVSRRMETGVISVNSNTSVRVATPFGGFKQSGVGRELGPRALDYYTEVKNVYLAT
                     EED"
     gene            3747..4514
                     /locus_tag="VFQ10_10730"
     CDS             3747..4514
                     /locus_tag="VFQ10_10730"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET9965859.1"
                     /translation="MPGRLEGKVAVVTGGAGGIGRETARRFAEEDARVCIVDLADEPG
                     QETASEIDGIYVHADVTDPEDVQRMYDETAERFGGIDVLFNNAGISPSDDDSILETGL
                     EAWERVQNVNLKSVYLCCKYGIPYLLERGGGSIINTASFVAVMGAATSQISYTASKGG
                     VLALSRELGIEFARRGIRVNALCPGPVNTPLLQEIFAKDPEKAERRLVHLPMGRFAEA
                     TEIAGAVLFLASDDASYVNASTFMVDGGLSGAYVTPE"
     gene            4527..5468
                     /locus_tag="VFQ10_10735"
     CDS             4527..5468
                     /locus_tag="VFQ10_10735"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET9965860.1"
                     /translation="MKSGISNSILDAIGNTPMLQVEGIYCKLEYLNPSGSIKARVAKY
                     MVERAENEGLLRPGDTIVEATSGNTGNALSMVAAVKGYRMLVVMPEGMSGERVAISRA
                     FGAEVMEVGHFHVNEALAKAKELGQEPGYFCPAQFESEWNVEENRIWLGPEILAQLPE
                     GVVPDALVMGVGTGGTLIGVGQAFREVNPRVKLFAMEPSESSTIMCGEVGDHLIEGIA
                     DGFVPGIFERHRDLVDTALTVDSEEAVEEMRRLARKCGLFVGPSSGANLIATRKVRGA
                     YPELETIITLFCDEGEKYISDHFAERDSVRVGREAYT"
     regulatory      5515..5632
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5739..7028
                     /gene="gcvPA"
                     /locus_tag="VFQ10_10740"
     CDS             5739..7028
                     /gene="gcvPA"
                     /locus_tag="VFQ10_10740"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET9965861.1"
                     /translation="MARFTPHTEADVQEMLDAIGVETLDDLFADVHPRYEGELDLPPT
                     LSEYEALREVDALAKENVWGLPIFLGAGAYDRIVPSAIGAIISRGEFLTSYTPYQPEI
                     SQGTLQSIFEFQSMISELTGLEISNASVYDGANAVVEAALMTARVTKREPKVAVSAGL
                     NPRYREVIETYRVEVVEQPFEDGATDFSNLPDDISGVFVQSPNFFGVVEDIGAASEAA
                     RDVEALCVAVCDPISLSVLEAPGNLGVDVAVGEAQPLGMPLIFGGPYAGYMATREDFV
                     RQLPGRITGETIDKDGKLAYVLTLRGREQDIRRAKANSNICTNQALTALAATVYTALM
                     GPEGLREVAELSISKAHYLAERLQASGFGLRYQEAPFLWEFAVELPDVQRANEALLEA
                     GMVGGLDLGDGAMLVAVTEKRTREELDAFVEVVADAL"
     gene            7018..8433
                     /gene="gcvPB"
                     /locus_tag="VFQ10_10745"
     CDS             7018..8433
                     /gene="gcvPB"
                     /locus_tag="VFQ10_10745"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET9965862.1"
                     /translation="MPFNLIRDKGREGRRGYTLPKPDVEMKELETILPGSVLRKQKPR
                     LAEVDEPTVIRHYTNLARENEGIDTTFYPLGSCTMKHNPRANEAAAVVPGFAGLHPLQ
                     PEAQSQGALRIMHELQGFLAEVSGLPYVSLQPLAGAQGELTSILMVKKFFDDAGDDGR
                     TAVLVPSTAHGTNPATASMAGFMAKEVGLDEHGCVDVDELREMVDDSTAALMITNPNT
                     CGVYERNIAEIAAVLHEAGAFLYMDGANMNANMGRMRPAEADVDIMHFNLHKTFSTPH
                     GGGGPGCGAIACSDDLAPFLPEPTVEKEGDAYRFVRPEKSLGRVAGFHGNFGQMLRAW
                     NYIRMHGPELRDVTDMAVLNANYVRAQLRGTYRIPYDELCKHEVVVQPPQGKRALDIA
                     KGLIDYGYHPPTIYFPLVVKEAMLIEPTETESKETLDDFIGTMLELAETSQEDLEEAP
                     THAPTRRLDETRAARQLKARW"
     gene            8436..9521
                     /gene="gcvT"
                     /locus_tag="VFQ10_10750"
     CDS             8436..9521
                     /gene="gcvT"
                     /locus_tag="VFQ10_10750"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET9965863.1"
                     /translation="MAKEGLKHTPLYDEHKDLGARLVDFAGYEMPVQYSGIQAEHKAV
                     RNHAGLFDVSHMGEVIFRGPDAESAVQRLVTRDVSRLDVGQAGYSAVCYEDGGTVDDV
                     IVYRRESDFLVVVNASNRAKDLEHFRENTNEFDVEVADESDHWALLALQGPEAAGLLQ
                     DLTETDLSGLKPFRFVEGVVDGAKCFISRTGYTGEDGFEIYLSGGDAPRVWRRLVEAG
                     ATPAGLGARDTLRLEAGMCLYGNELDAKTTPLEAGISFAVHLDKEQRFIGQRALLLED
                     TEGLRKKLVGFEMEGRGIARHGHHVTSGAEIVGEVTSGTKSPTLEKAIGLALVDPGVE
                     DRFEIVIRDNPVPAKTVPLPFYKRTGG"
     gene            9531..9908
                     /gene="gcvH"
                     /locus_tag="VFQ10_10755"
     CDS             9531..9908
                     /gene="gcvH"
                     /locus_tag="VFQ10_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET9965864.1"
                     /translation="MNIPEDLQYTESHEWVRIEGETATIGITDHAQDELGDVVFVELP
                     GEGDTFDAGESFGTVESVKAVSDLYAPVGGEVVEVNSALEDAPENINEDPYGEGWIVK
                     LRTSDEPHLLSPQEYEQVVAEES"
     gene            complement(10334..10975)
                     /locus_tag="VFQ10_10760"
     CDS             complement(10334..10975)
                     /locus_tag="VFQ10_10760"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET9965865.1"
                     /translation="MRATLQPGLTNRLTKYLRTTQQLIEEGRDAVSSKELGDFTGINP
                     VQVRRDLNAIGFSGTRGVGYQAYDLVEAVRNILGLRQTYNIALVGAGNLGSAIAASTI
                     LPKRGFVIHDVFDDNPEKIGRTVGNVVVKHIDELKKSVTEAEEIIGIIATPASSAQEV
                     ANLMVDSDIRVILNYTDVLLHVPSHVDVHRIDPTAQLMHTLYYLTQAEGQEAS"
     gene            11181..12146
                     /locus_tag="VFQ10_10765"
     CDS             11181..12146
                     /locus_tag="VFQ10_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HET9965866.1"
                     /translation="MEYRRLGKSGVKVSEISLGSWLTYGGSVAEEQAKSCIDRAYELG
                     INFFDTANVYMRGAAEEIVGQALKDFERHSYFLATKVYFPMGEGPNDRGLSRKHITEQ
                     CNASLKRLGVDYVDLYQCHRYDENTPLEETMRALDDLVRQGKVLYIGVSEWTADQISD
                     ALRIAGEMNLDRIVSNQPQYNMIQRRIEGEVIPLCEREGVGQVVFSPLAQGILTGKYR
                     SGDAPVEGTRAADPESNRFMQQLMNEEVLSAVDALRSVASEAGLSMPQLALAWVLRLG
                     NVSSAIIGASRPEQVDDNAAASGVELSADMISEIDGILNGVIQRT"
     gene            12462..13355
                     /locus_tag="VFQ10_10770"
     CDS             12462..13355
                     /locus_tag="VFQ10_10770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9965867.1"
                     /translation="MTETDSGVRVRQMDDADLERILELRSIVRWSADPRAFDLLRGVR
                     DARWAVAEAPDGELAGMVGAVPLGDIGVLCHLAVHDGYRGLSLGALLSTWAVAYLRSR
                     GAKTIRLYSTLRAEGLYRSLGFEEVTPRTVYRLEEGSWRLRVPDQVDGHRVETLTFGD
                     LPELYGVDRWSYGADRSALLFATLRLHPGRGLVVRDSTGRIKGYLIRSVRGRATRIGP
                     FLASTPDVARLLLARALSATGGTPVQVTVPGPAGCHAHSLLQEFGFEGTEDRMRMELG
                     EKTATCRTGVAHYGTTPYLAT"
     gene            13531..15225
                     /locus_tag="VFQ10_10775"
     CDS             13531..15225
                     /locus_tag="VFQ10_10775"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET9965868.1"
                     /translation="MRMSTLFGLTLREAPSGVEVASHGLLLRAGFIRQLAAGIFSYLP
                     LAKRSIGKIEKILREEMEAAGGQELSLPVVQPADVWKRSGRYDTTGQELVRLSDRRNR
                     EMVLAMTHEEIVAGLAASEVDSYQRLPRLVYQIQTKFRDDPRPRAGLIRAREFTMKDA
                     YSLDRDERGLDQQYSTLYKAYFKIFARCGLPAIAVGADVGIMGGSMAHEFMYLTPIGE
                     DTILICDDCGYTANRQVAKFRKPISGAEQQRPVEKVPTPGASTIETLAHSLEIPKSST
                     AKALFQVATVEGSERFVLAVVRGDMELNETKLANAVGASGLRPALPEEVRAVGAEPGY
                     GSPLGVEGAIVVVDDAIPASSNLVAGANEEGSHFLNVNYGRDFEADIVADIAAAENGS
                     LCPECGNTMRAFRGVEVGNIFKLGTRYSEALGASFLDADGKRKSVVMGSYGIGLGRLL
                     ACIAEEHHDENGLVWPISVAPYHVHIVSADAGEVADGLNERLVSAGVEVLYDDRRESL
                     GAKFKDADLIGAPIRLTLTPRSMEKGGVEIKARGSSEGYVVPIEGAVAALRREISD"
     gene            complement(15270..16265)
                     /locus_tag="VFQ10_10780"
     CDS             complement(15270..16265)
                     /locus_tag="VFQ10_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET9965869.1"
                     /translation="MIGSGDIEVARRRLQGVALHTPLVPCPRSEDDRALYFKLESLQP
                     TGAFKLRGAYNKISSLSPEDRRRGVVAHSSGNHAQAVAYAARVLEMRAVVVMPRGASR
                     VKLDATAELGAEVVLVGPDSAERSRKAEELAVEHGYVPVPPYDDEVIIAGQGTIGPEI
                     LEDLPDVETVLVPVSGGGLIGGTSAAIKLARPEVRVIGVEPELAADARASLESGRLVE
                     FPADQVARTVADGLRVQRLGEAPFEHIRAFVDEIVAVTEEEILEAMRRLAIRVKLVAE
                     PSGAVTFAAYLFHREELPTSRLTVAVISGGNAESDLLAEVLAGSAFEGVRNAAAR"
     gene            16578..17378
                     /locus_tag="VFQ10_10785"
     CDS             16578..17378
                     /locus_tag="VFQ10_10785"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HET9965870.1"
                     /translation="MAGTGGMPEATSIGAPQIEGDLAGATDASGVPFVAKTQASGGSG
                     YDADTVLAVRYEANEDYERAVIDLGTGLEPGGPVPEWSLVSQEGKGLLRVTLPSASAT
                     CVSDGEFGDGLLESFHVVRAPEGGMFMDIFAREAFLYKVVELGDPARLVFDFKPKGDL
                     KAPLPAESGDTVLVEPRSHASISDPLTVNGYSRNSEAANTITLIDSEGKILVRKTVLS
                     NDRDHTWGYFETTLDLPPFSGKGTLRVGTESARNGSFEGTEIPLWAGQ"
     gene            complement(17470..>17738)
                     /locus_tag="VFQ10_10790"
     CDS             complement(17470..>17738)
                     /locus_tag="VFQ10_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965871.1"
                     /translation="EPDWAAVIHVAVAPVYYHNYMLGELISAQLRNYLESHITRGPFY
                     QNEVAGRYLLESFFGPGARENWRDTVLRATGAPLNPGYFVKSLG"
BASE COUNT         3446 a         5488 c         5729 g         3075 t
ORIGIN      
        1 gatgatgctc tcgcacatcc tcctgcgccg caatgagccc gacctcgaac gcccgtaccg
       61 tacgccgggc ggtatggcga cctcgggcgt ggcgctcgtg ctggcggtgc tggccgtcat
      121 cgcgaccttt atcgtggacc cgacggcggc gtttatcacg ctgggtgtct acgtgctctt
      181 cctcgcctac ttcggcttct acagccgcca tcacctcgtc gcccaggcgc cggaagaaga
      241 gttcgccact atcgagaagg ccgagtccga gctggccgga agctagagag gagcaagaat
      301 tggacacgat tcaggcggct ggacgcctcg acctcgacac cctgcgctcc gaggtcgaag
      361 ctggaacgat cgacaccgtc ctcctggcga tgacggatat gcagggccgg ctccagggca
      421 agcgtctcac agcgactcac ttcctcgatg aggtggtcga gcacgacgcc gaaggctgca
      481 actacctgtt ggccgtggac gtggagatga acaccgtaga gggatacgcg atgtcctcct
      541 gggagcgcgg atacggcgac ttcgtcttca agcctgattt ggacaccctc aggcgagtcc
      601 cctggcagga ggggacggcg ctcgtgactt gcgatctgct ctgggaggac ggctctccgg
      661 tcgtcgcctc gccccggcag gtcctcaagc gccagctagg gaggctctcc gaacgtggcc
      721 tggaagcctt cgtagggacc gaactcgagt tcatcgtctt catggactcc tacgaggaag
      781 cctggcgtaa agggtacaga gatctggagc cagccaacta ctacaacgtc gactactcgc
      841 tcctgggaac ggcgcgcatc gagccgctgc tgcgccgcat ccgcaactcg atggcggggg
      901 ctgggctcgc ggtggaggat gcgaagggcg agtgcaactt cgggcagcac gagatcaact
      961 tccggttcga ccgcgctctc accaccgcgg acgggcacgc gatctacaag aacggcgcca
     1021 aggagatcgc cgcccaggag ggctgctcca tctcctttat gccgaagttc gacgagcgcg
     1081 agggtaactc ctgccacatc cacctgagcc tgcgcgcgga ggacggcgca cccgtcttcg
     1141 ccgaagacca cggtttctcg gacaccttca accacttcct cgctggccag ctcgcctccc
     1201 tgcgcgaact cacgctcttt ttcgccccga acataaactc ctacaagcgt tacgcgaagg
     1261 gttccttcgc ccctactgca gtcgcctggg gcaaggacaa ccgcacttgt agcatgcgcg
     1321 tcgtcggcca cggcggctcc ttgcgcgtgg agaaccgcct gcctggcggg gacgtgaacc
     1381 cttacctggc gatcgcagcc atgatcgccg ccggtctgca cggtatagag aatgaactct
     1441 ccctggaaga cgagttcgaa ggcaacgcct acgaggcgga caaaccgcac gtaccgacga
     1501 acctgcgcga ggcgcaggag ctcttcgccc agagtgagac ggcccgcagt gcgttcgggg
     1561 atgaggtcgt cgagcactac ctgaacatgg cgcgcgtcga gatcgaagcc ttcgaggccg
     1621 ccgttaccga ctgggaacgc taccggaact tcgaacgcct gtgaaaggga agtccaggcc
     1681 gaatatcggt gtcacagcgg ctacggagca gatcaactcc ggcgtctggg aggagatacc
     1741 ggcggtcatc agcccggcgc gctacgtaga ggccgtgcag cgcgccggcg cgcgtgcagt
     1801 cctgctgcct ccagatccgg aagatgcgga ggaccctggc ggggtgctcg accttctcga
     1861 cgcgctcgtc gtcacaggag gggctgggga cctggatcct gccctctacg gagacgaaag
     1921 gcaccccgag acaggacccg tacaggagga gcgcgacgcc tacgaactct cgctggtgcg
     1981 cggagccctg gagcgcgaga tgcccgtcct cggcatctgc cgcgggatgc aggtcctcaa
     2041 cgtcgcctac ggaggcacca tagaacaaca cctccccgac gtgctgggcc acgaagaaca
     2101 tcgccgcacg ccaggcacct tcgccgacca cgaggtgcgc ctcgtccccg actcactcgc
     2161 cgcccgcgcc gccggctcgg agaggacgcc ggccaagtct caccaccacc aggggataaa
     2221 ggcggtaggg gaagacctcc aggttacggg ctgggctgtc gaagacgatg cggtggaggc
     2281 cctcgaagac ccatcctgtc ctttcgtgct cggcgtgctc tggcaccccg aagaagatga
     2341 gaagagccag ctcataaagg ccctagtttc ggaggtctcg tagatgctcg aagtcctgaa
     2401 cccggcgacc gaagaggtgg tagagagcct cgaacccgcc accgaggatg acgccgacgc
     2461 ggccatcgcc cgcgccagag acgccttccc ggcgtggaga gatgtgcaac ccggcgatcg
     2521 ctcgcgtctg ctcaggcgtc tcgccgacgc actcgatgcg gaacaggaga acctggcgca
     2581 actggagtcg aagaacaccg gcaagcccat aggcgactcc cgcggcgaga tggcgatggt
     2641 agcggagact ttccactact acgcggggat gccggagcgt ttgctcggcg acaccattcc
     2701 ggtctctggg ggcgtcgacg tgacctttcg cgaaccgtta ggcgtcgtgg gcctcatcgt
     2761 cccttggaac ttcccgctca ccatagcgag ctggaagatg gccccggccc tggcggccgg
     2821 aaacacgctc gtcctcaagc ctgcggagct cacgccgctg agtgccatgg agttcgagcg
     2881 catagccctc gaagcaggca tccccgaggg cgtcgtcaac gtcgtggtcg gtcctgggag
     2941 caaggtcggg aagaggctcg tcgagcaccc ggacgtagcc aaggtcgcct tcacgggttc
     3001 cacggaggtc ggcaagggca tcatggcggg ggccgcaggc accataaagc gcgtcaccct
     3061 ggagctgggg ggcaagtccg cgaacgttgt cttcgccgat gccgacctgc agaaggccgc
     3121 cgcttcggcg cccatcgccg tcttcggtaa cgccgggcag gactgctgcg cccgctcgcg
     3181 catcctggtc gagaaaggcg ccttcgacga gttcctctcg ctcatgcagc ccgtggtaga
     3241 gggtttccgg gtcggggacc cgttggacga ggagacggag atgggacctc tgatctccgc
     3301 cgaccaccgc gagagagtcg ccgcatacgt cccagaagac gcacccgtgg cgatccgggg
     3361 cgaggcaccg gaaggctccg ggttctggtt ccccccgacg gtgctggctc cgctctccaa
     3421 cggcgatcgg gcagccaggg aggagatctt cggacccgtc gtcgccgtga tccctttcga
     3481 ggacgaagag gacgccgtgc gccaggccaa cgacaccgtc tacggcctct caggttcgat
     3541 ctggaccgag aacggcgccc gcgccctgcg cgtgtcacgt cgcatggaga cgggcgtgat
     3601 ctccgtcaac tccaacacct ccgtcagggt ggccaccccc ttcggcggct tcaagcagtc
     3661 cggcgtcgga cgcgagctag gcccgcgcgc cctcgactac tacacggagg taaagaacgt
     3721 ctacctggcc acagaggagg actgatatgc ctggacgcct ggaagggaag gtcgccgtcg
     3781 taaccggcgg cgccggtggc ataggccggg agaccgcccg ccgcttcgcc gaggaagacg
     3841 cccgcgtctg catagtggat ctggcagacg agccgggtca ggagacggcc tccgagatag
     3901 acggcatcta cgtccacgcc gacgtaaccg accccgaaga cgtgcaacgc atgtacgacg
     3961 agacggccga gcgtttcggc ggtatagacg tgctcttcaa caacgccggc atctcccctt
     4021 ccgacgacga ctcgatcttg gagacggggc tcgaagcctg ggagcgcgtc cagaacgtga
     4081 acctcaagag cgtctacctg tgctgcaaat atgggatacc ttacctattg gagcggggag
     4141 gaggctcgat catcaacacc gcctctttcg tcgccgtgat gggtgctgcc acatcccaga
     4201 tctcctacac cgcatcgaag ggcggcgtgc tggccctcag ccgcgagctc gggatcgagt
     4261 tcgccaggag ggggatacgc gtcaatgcgc tctgtccggg cccggtgaat acaccgcttc
     4321 tgcaggagat cttcgccaag gacccggaga aagcggagcg caggctcgta cacctgccga
     4381 tgggacggtt cgcggaggcg acggagatcg cgggcgccgt cctcttcctg gcctccgacg
     4441 acgcttccta cgtcaacgcc tccaccttca tggttgacgg cggcctctcg ggggcatacg
     4501 ttacccctga ataggaggtg gtcggcatga aaagcgggat ctccaacagc atcctcgacg
     4561 ccataggaaa cacgccgatg ctccaggtcg agggcatcta ctgcaagctc gagtacctga
     4621 acccttccgg gtccatcaaa gcacgcgttg cgaagtacat ggtcgaacgc gccgagaacg
     4681 aggggcttct gcgaccgggc gataccatcg tcgaggccac ttcgggcaac acgggcaacg
     4741 ccctctcgat ggtcgccgcg gtcaagggat acaggatgct cgtggtgatg cccgagggga
     4801 tgagcggcga gcgcgtcgcc ataagccgcg ctttcggggc cgaggtgatg gaggtcgggc
     4861 acttccacgt caacgaagcc ctggccaaag caaaggagct cgggcaggaa cccggatact
     4921 tctgcccggc ccagttcgag tcggagtgga acgtggagga gaacaggatc tggctcgggc
     4981 cggagatact ggcccagcta cccgagggcg tcgtccccga cgccctcgtt atgggggtcg
     5041 ggacaggggg aactctcatc ggcgtcggac aggcgttccg tgaggtcaac ccgcgcgtca
     5101 agctcttcgc gatggagccc tccgagtcat cgaccatcat gtgcggcgag gtgggggatc
     5161 acctgatcga aggcatagcc gacggcttcg tcccaggcat cttcgagcgc cacagggact
     5221 tggtcgacac cgccctgacc gtcgactccg aagaggccgt agaggagatg cgccgcctcg
     5281 ccaggaaatg cggcctcttc gtcggccctt cgagcggcgc caacctcata gccaccagga
     5341 aggtacgcgg ggcctacccc gagctcgaga ccatcatcac actgttctgc gacgaggggg
     5401 agaagtacat aagcgatcac ttcgcagagc gagactcggt cagggtaggg cgcgaggctt
     5461 acacgtagag aagcgtcaac ccacgcccct tatgctatag tccgaacttg cccgatgagg
     5521 gccgcgggag agagtcgttt gaggtgtggg ggagacctcg tgacggccgc cgaaggtgaa
     5581 agggttgccg gggcaatctg aatctctcag gcaaaaggac cgcggaccgg agggcgtcct
     5641 ggagagtccg gcacggaccg gcgccgacgg ggtaacactc tcaggcttgg gacagggcgg
     5701 aaaaagcaac gctcacccga gacagggggt agaggcgcgt ggcgcgattc acaccgcaca
     5761 cagaggccga tgtacaggag atgctggacg ccatcggggt cgagacccta gacgacctct
     5821 tcgccgacgt ccacccgaga tatgaaggag agctcgacct tccgccgacc ctctcggagt
     5881 acgaggcgct gagagaggta gacgccctgg ccaaggagaa tgtctggggg ctaccgatct
     5941 ttctcggtgc gggggcctac gataggatcg taccctccgc cattggcgcg atcatctcgc
     6001 gcggtgagtt cctgacctct tacacccctt accagccgga gatcagccag ggcaccctgc
     6061 agtccatctt cgagttccag tccatgatct ccgaactgac agggttggag atctccaatg
     6121 cctccgtata cgacggcgcg aacgccgtcg tggaagccgc cctcatgacg gcgagggtga
     6181 cgaagcgtga acccaaagtc gcagtctccg ccggcctcaa cccccgctac cgcgaggtta
     6241 tagagaccta ccgcgtggag gttgtggaac aaccgttcga agatggcgcc acggacttct
     6301 ccaatctgcc cgacgacatc tctggcgtct tcgtgcagag cccgaacttc ttcggggtcg
     6361 tcgaggatat aggggccgcc tcggaggcgg cgcgcgatgt ggaggcgctc tgtgttgcgg
     6421 tgtgcgaccc gatctcactc tccgtgctcg aagcccccgg gaacctgggg gtcgatgtgg
     6481 ccgtgggcga ggcccagccg ctcgggatgc cgctcatctt cggtggccca tacgcaggct
     6541 acatggcgac tagggaggat ttcgtccgcc agctcccggg acgtatcacg ggagaaacca
     6601 tagacaaaga cggaaagctg gcctacgtcc tcacactgcg ggggcgcgag caggacatca
     6661 ggcgcgcgaa ggcgaactcc aacatctgca ccaaccaggc actcacggcc ctggccgcga
     6721 ccgtctacac ggccttgatg ggaccggaag gactgcgcga agtggccgag ctctccatct
     6781 cgaaggccca ttatttggca gagaggcttc aggcttcagg cttcgggctt cggtatcagg
     6841 aggcaccttt cctctgggag ttcgccgtgg agttgcccga tgtccagcgg gccaatgagg
     6901 ctttgctgga ggccgggatg gtgggaggcc tggatctggg ggacggggcg atgctcgtcg
     6961 ctgtgacgga gaagcgcacc agggaagaat tagacgcctt cgtggaggtg gttgcagatg
     7021 ccctttaacc tgattcggga caaaggccgt gagggccgca ggggttatac cctcccaaag
     7081 ccggacgtgg agatgaaaga gcttgaaacc atactgcctg ggtcggtgtt gcgtaaacag
     7141 aagccacgcc tagccgaggt ggacgagcct accgtgatcc gacactacac aaacctcgcg
     7201 cgcgagaacg aaggtatcga cactacgttc taccccctgg gctcgtgcac gatgaagcac
     7261 aacccccgcg ccaacgaggc agccgccgtc gtccccggct tcgcggggct ccatccgctc
     7321 cagcctgaag cccagtccca gggcgcccta cggatcatgc acgagctaca gggcttcctc
     7381 gccgaggtgt ccgggctacc ttacgtctcc ctgcagcctc tcgccggggc gcagggggag
     7441 ctgaccagca tcctgatggt caagaagttc ttcgacgatg ccggtgacga tggacgcacc
     7501 gcagtcctcg ttccttcgac ggcgcacggg accaaccctg cgacagcctc gatggctggg
     7561 ttcatggcaa aggaggtcgg gctcgacgag catgggtgcg tcgacgttga cgagctgagg
     7621 gagatggtag acgattcgac ggcggcgctc atgatcacca accccaacac ctgcggcgtc
     7681 tacgagcgga acatcgccga gatcgccgcc gtcctgcacg aggcgggcgc cttcctctac
     7741 atggacggcg cgaacatgaa cgcgaacatg ggccgtatgc gcccggccga agccgacgta
     7801 gacatcatgc acttcaacct gcacaagacc ttctccaccc cccatggagg cggcggacct
     7861 ggctgcggcg ccatcgcctg ctcggatgac ctcgcgccct tcctccccga gcccacggta
     7921 gagaaggagg gcgacgcgta ccgtttcgtt cgtcccgaga agagcctcgg tcgcgtggca
     7981 ggcttccacg gcaacttcgg acagatgctg agagcctgga actacatcag gatgcacggc
     8041 cccgagctca gggacgtgac ggacatggct gttctgaacg ccaactacgt gcgggcacag
     8101 ctcaggggca cctatcgcat accttacgac gagctgtgca agcacgaggt cgtcgtgcag
     8161 cctccgcagg gcaagagggc tctggatatc gccaaggggc tgatcgacta cgggtatcat
     8221 ccgccgacga tctacttccc cctcgtcgtc aaggaggcca tgctcataga gccgaccgag
     8281 acggagtcca aagagaccct cgacgacttt atagggacga tgctggaact ggccgagacg
     8341 agccaggaag acctcgaaga agctccaacc cacgccccga cgcgccgcct cgacgagaca
     8401 cgtgccgcca ggcaactcaa ggccaggtgg tagcggtggc taaggagggc ctcaagcaca
     8461 ctccgctcta cgacgagcat aaggacctcg gggcgcggct cgttgacttc gccggctacg
     8521 agatgcccgt gcagtattcg ggcatacagg ccgagcacaa agcggttcgt aaccacgccg
     8581 ggctcttcga cgtttcgcat atgggcgagg tcatcttccg cggccctgac gccgaaagcg
     8641 ccgtccagcg cctcgtaacc cgtgacgtct cgcgcctgga tgtagggcag gcaggctact
     8701 cggcggtctg ctacgaggac ggggggacgg tggacgatgt gatcgtctac cggcgcgaaa
     8761 gcgacttcct cgtcgtcgta aacgcctcga accgcgcgaa ggatctcgaa cactttaggg
     8821 agaacaccaa tgaattcgac gtggaagtcg ctgacgaatc cgaccactgg gcgcttctag
     8881 cgttgcaggg acccgaggcg gcagggctcc tgcaggacct caccgaaacg gatttgtcgg
     8941 gcctcaagcc cttccggttc gtcgaaggag tggtcgacgg tgcgaagtgt ttcatctccc
     9001 gcaccggcta cacgggtgag gacgggttcg agatctacct ttccggaggc gacgccccgc
     9061 gcgtctggcg ccgcctggta gaggcaggcg ccaccccggc aggactcggc gccagggaca
     9121 cgcttcgcct cgaagcgggg atgtgcctct acggcaacga gctcgatgca aagaccacgc
     9181 ccctcgaagc cggcatctcc ttcgcggtgc acctcgacaa ggagcagcgt tttatcgggc
     9241 agagggcgct cttgctcgag gacaccgagg ggctgcgcaa gaagctcgtc ggcttcgaga
     9301 tggaggggcg tgggatcgca cgccacggcc accatgtaac cagcggggct gagatcgtcg
     9361 gtgaggtcac tagcggcacc aagtctccca ccctcgagaa ggcgataggc cttgcgctcg
     9421 tagatccagg ggtggaagat aggttcgaga tcgtgataag ggataaccca gtaccggcga
     9481 agaccgtgcc gctaccgttc tacaagcgca ccggaggata ggggagagcc ttgaacatac
     9541 ctgaagacct tcagtacaca gagagccacg agtgggtccg catagagggc gaaaccgcga
     9601 ccatcggtat cacggaccac gcgcaggacg aactgggcga cgtcgtcttc gtcgagttgc
     9661 ccggtgaagg agataccttc gacgccggcg agtccttcgg caccgtcgag agcgtcaagg
     9721 cagtctcgga tctgtacgct cctgtcggcg gcgaggtagt cgaggtcaac tcggcactcg
     9781 aagatgcccc cgagaacata aatgaggacc catacgggga gggctggatc gtcaagctcc
     9841 gcacctcgga cgaaccccac ctcctctccc cccaagagta cgagcaggtc gtggccgaag
     9901 agtcatagcc taaacgcaag atccacactc cctggcccag cttgccttag atacccacta
     9961 tatctttatg tgcattttgc agatatctac aaaaagtttc taaattctct gttccaaagc
    10021 ctttacaaag tgtaacgagc gatgtatcat ccaatcagtc ggttggtgcg aaactaaacc
    10081 ggcacaatta aagagtccgg ggcgctactc ctcctttccc tttccccacc tatcacccct
    10141 aggtgcgccc cgggctcttc ctctgtttca agctagtcag ccggtcagca agtcgcctgt
    10201 accaggcgac ttgtcagcat ttcagccagg gctgtgagtg gcgcttccgt gaagcggctg
    10261 cgtgcggttc tccacagaga gaagtggtcc caggctgagt accgaggacc actagctgac
    10321 cagctgaaat gctttaagac gcctcttgcc cctcggcctg ggtcagataa taaagcgtgt
    10381 gcattagctg ggcggtgggg tctatacgat ggacgtccac gtgagatggg acgtggagga
    10441 ggacgtcggt gtaattgagg atgaccctga tatctgagtc gaccatcagg ttggcgacct
    10501 cctgggccga ggaggctggg gtcgcgatga tgccgatgat ctcctctgcc tcggtgacgc
    10561 tcttcttgag ctcgtctatg tgcttgacga cgacgttgcc cacggtgcgt ccgatcttct
    10621 ccgggttgtc gtcgaagacg tcgtgtatga cgaagcccct cttggggagg atggtgctcg
    10681 ccgcaatggc gctgccgagg ttgcccgcgc cgaccagcgc gatgttgtag gtctgcctga
    10741 gacccaggat gttcctcaca gcttcgacga gatcgtaggc ctggtagccc accccgcgag
    10801 tgcccgagaa gcctatggcg ttcagatccc tgcgtacctg cacggggtta atgcccgtga
    10861 agtcgccgag ctccttgctt gagaccgcgt cacggccttc ctcgatgagc tgctgcgtgg
    10921 tcctaagata cttcgtcagc ctgtttgtca ggccgggctg tagcgttgct cgcacctttg
    10981 ctcctgctcc ttcttcgtgg atcgaatatc gatgggagca ttgtaacaaa ataggccgtt
    11041 cttgcaataa aggtgctctt aaccgacaac aaatcagtaa gcaaggccgg ttacagcgcg
    11101 atgggccggt aggatagggg atttacgggc cgtcaggcgt gtactattgg taccttgttc
    11161 tctgtacact ggaggttttg gtggagtaca ggcggttggg taagagtggt gtgaaggtca
    11221 gcgagatctc tcttgggagc tggctgacct acggcgggag cgttgccgaa gagcaggcga
    11281 agtcctgtat agacagggcg tacgagctcg gcatcaactt tttcgacacg gccaacgtgt
    11341 acatgcgtgg ggcggccgag gagatcgtgg gccaggcatt gaaagacttc gagcgccact
    11401 cgtactttct ggcgacgaag gtgtacttcc cgatggggga gggaccaaac gacaggggcc
    11461 tctcgcgcaa gcacataacc gagcagtgca acgcgtcgct aaagcggctt ggggttgact
    11521 acgtcgacct ttaccagtgc caccgctacg atgagaacac accactagag gagaccatga
    11581 gggcgctcga cgacctggtg cggcagggga aggtccttta catcggagtc tcggagtgga
    11641 cggccgacca gatctccgac gcgctgagga tcgctggtga gatgaacttg gatcggatcg
    11701 tctccaacca gccgcagtac aacatgatcc aacgccggat cgaaggcgaa gtcatacccc
    11761 tttgcgagcg cgaaggcgtt ggccaggtgg tcttctcacc gctcgcccag ggcatactga
    11821 cgggcaagta tcgttcaggc gatgcccccg tggagggcac ccgcgcagcc gacccggaat
    11881 ccaaccgctt tatgcagcag ctcatgaacg aggaagttct ctcggccgtg gacgcgttgc
    11941 gctccgtcgc gtctgaagca ggcctcagca tgccgcagct cgccctcgct tgggtgttgc
    12001 gcctggggaa cgtgagcagc gcgatcatcg gcgcctccag acccgagcag gtcgacgaca
    12061 acgccgcagc ttccggggtg gagctctccg cggatatgat ctcggagata gacgggatac
    12121 tgaacggggt gatacagcgc acttagggcc gttcagcgaa tcttctctca gaatccttcc
    12181 atgtcagcgt gccatgttgt agcattgctg gtgtggagcg agatcacata gggtttacag
    12241 cgagctcctt tacaggacgg ttccctcgac cgtccctgcc ccgaccgtcc ctctagggcg
    12301 agttcggggc atccgcgtct ctagaggttt ctctgaaacc ttttgcgcgg aacgttctct
    12361 atccatgaat aaagtgagat cgagggaaaa ccttgctggc attcagcgcg agagatgaca
    12421 acgaagtaga aaagactccc cccaagaacg tgttacggac agtgaccgag acggactcgg
    12481 gcgtccgcgt caggcagatg gacgatgctg acctggagcg catactcgag ctacggagca
    12541 tcgtacgctg gtcggctgac ccgagggctt tcgacctcct acgcggcgtg cgcgacgccc
    12601 gctgggctgt ggccgaggcg cctgacggcg agctggcggg tatggtcggt gctgtgccgc
    12661 tcggagacat cggcgtcctc tgccatctcg cagtccacga cgggtacagg ggcctcagcc
    12721 tcggcgccct gctctccacc tgggccgtag cctacctcag aagccgtggc gcgaagacga
    12781 tcaggctcta ctcgaccctg cgggcggagg gactgtaccg gtcgctcggt ttcgaggagg
    12841 tcacgccgcg caccgtttac cgtcttgagg aaggatcttg gagactccgc gttcccgatc
    12901 aagtagacgg gcacagggtc gagacgctca cgttcgggga cctgcccgag ctctacggcg
    12961 tggaccgctg gagctacgga gccgaccgtt cggctctgct cttcgccacc ctgagactac
    13021 atccaggacg cggtctcgtc gtccgcgact cgacgggccg tatcaagggc tacctgatcc
    13081 ggagcgtcag gggccgcgcg acccgtatcg gccccttcct ggcttccaca cccgacgtgg
    13141 cccgcctcct gctcgcccga gctctatcgg cgaccggagg tacgcccgtc caggtcacgg
    13201 tccccggtcc ggccggatgc cacgcgcact ccctcttgca agagttcggc ttcgagggca
    13261 cagaggaccg gatgcggatg gagctgggcg agaagacggc cacctgccgt accggcgtcg
    13321 cccactacgg cacaacaccg tatctagcga cttaggcttc aggtatcagg cgacaggttc
    13381 tcgtggagaa gttatctggc ttaggccatc cactgtagaa aggagtcggc gtgtttgatt
    13441 gcgtactgag aatcaggggt cggcaagtgg agtgggacga ctccctggcg gtcctgatgg
    13501 ctgaaatctg tagcctgtag cctgttcgcc atgcgcatga gtacactctt cggcctgacg
    13561 ttgcgtgagg cgccttccgg ggtggaggtt gccagccacg ggctgctgct cagggccggg
    13621 ttcatcaggc aactggcggc cgggatcttt tcgtatctgc cactggcaaa gcggtctatc
    13681 ggcaaaatcg agaagatctt gcgggaggag atggaggcgg ctggcgggca ggaactgtcg
    13741 ttgcccgtcg tgcagccggc cgatgtatgg aagagatccg gccggtacga cactacggga
    13801 caggagctgg tcaggctcag cgaccgaagg aacagagaga tggtcctggc gatgacccac
    13861 gaggagatcg tcgccggcct tgctgcgagc gaggtggact cttaccagag gttgcccagg
    13921 ctcgtgtatc agatccagac caagttccga gacgatccgc ggccgagggc cggcctgatc
    13981 cgggcgcgcg agttcaccat gaaggacgca tacagcctcg acagggatga gaggggcctc
    14041 gaccaacaat acagcacgct ctataaggca tacttcaaga tcttcgcccg gtgcggacta
    14101 ccagccatag ccgtcggggc cgacgtgggc atcatgggtg ggagcatggc ccacgagttc
    14161 atgtacctta cgcctatcgg ggaggacacc atcctcatct gcgacgattg cggttacacc
    14221 gcgaaccgcc aggtggcgaa gttccgcaag cctatctccg gggccgagca gcagagacct
    14281 gtagaaaagg tcccaacacc tggcgccagc accatagaga cgctggccca ctccctggag
    14341 ataccgaagt ccagcacggc caaggcgctc ttccaagtcg ccacagtcga gggctccgag
    14401 cgattcgtcc tcgccgtcgt gcgtggcgac atggagctca atgagaccaa gctggccaac
    14461 gcggtggggg cgagcggact gaggcccgcg ctccctgagg aggtcagggc cgtgggagcc
    14521 gagcctggat acggctctcc gctcggggtg gagggggcga tcgtggtcgt tgacgatgcg
    14581 ataccggcct cctccaacct ggttgcgggc gccaacgagg agggttccca cttcctgaac
    14641 gtcaactacg gcagggactt cgaggctgat atcgtcgctg acatagcagc ggcggagaac
    14701 ggctcactct gccccgagtg cggcaacacc atgcgagcct ttcggggtgt cgaggtcggg
    14761 aatatcttca agctgggcac gcgctacagc gaggccctcg gcgcgagctt cctcgacgca
    14821 gacggaaagc gaaagtccgt ggtgatggga tcttacggca tcgggctcgg gcggctactg
    14881 gcctgcatcg cggaggaaca tcacgacgaa aacggcctcg tctggcccat ctccgtcgcg
    14941 ccgtatcacg tccacatcgt ctcggccgat gcgggagagg tggccgacgg gctcaacgag
    15001 aggttggtct ctgcaggcgt cgaggtgctt tacgacgatc ggcgcgagag ccttggggca
    15061 aaattcaagg atgctgatct tatcggggct ccgatacgcc tgaccctgac accccgctcc
    15121 atggagaaag ggggagtgga gatcaaagcc cgcggaagct ccgagggcta cgtagttccc
    15181 atcgaagggg ctgtcgcagc actcagaagg gagatctctg actagggtcg atataccccc
    15241 tggcgatccc gttacgtgag aacccggcct tatcttgcag cagcatttcg aacgccctcg
    15301 aacgcagagc cggcgaggac ctcggcgagg aggtctgact cggcgttgcc gccactgatc
    15361 acggccacgg tgaggcgaga ggtgggcagt tcttctctgt ggaacaggta ggcggcgaac
    15421 gtcacagccc cgctcggctc tgcgacgagc ttgacccgta tggccaggcg gcgcatggct
    15481 tccaggatct cctcctccgt tacggcgacg atttcgtcga cgaaggcgcg gatatgctcg
    15541 aagggcgcct cgccgagtct ctgcacgcgc aaaccgtcag caaccgtgcg ggcgacctga
    15601 tccgccggga actcgaccag ccttccactc tccaggctgg ctctggcgtc ggctgcaagc
    15661 tcgggttcga ccccgatcac tcgcacttca ggacgggcca gcttgatggc ggccgaggtc
    15721 ccaccgatga gcccgccgcc gctgacgggc acgagcacag tctccacatc gggcagatct
    15781 tcgaggatct ccggcccgat agtgccctgg ccggcgataa tgacctcatc gtcgtacgga
    15841 ggcacgggaa cgtagccgtg ttccaccgca agctcctccg ccttgcgaga gcgctctgcg
    15901 ctgtcggggc cgaccaggac gacctcggcc ccgagctccg ccgtcgcgtc gagcttcaca
    15961 cgagatgccc cccgcggcat tacgacgacg gcccgcattt ccaggacgcg ggcggcgtag
    16021 gctacggcct gggcgtggtt cccactggag tgggcgacga ccccgcggcg gcgatcctca
    16081 ggggagaggg aagagatctt gttgtacgcg ccgcgcagct tgaaagcgcc cgtaggctgg
    16141 aggctctcca gcttgaagta cagtgccctg tcatcctcgc tgcgtggaca cggtacgagc
    16201 ggcgtgtgga gcgccactcc ttgcaaacgc ctccgcgcga cctctatgtc ccctgatccg
    16261 atcatgtttt catgctcctg tgcgccgaaa gtgaggtatc gtggctactc cagcatcgta
    16321 acaggagcgc aacaccgcgt taaacatctc gcaaggtgaa cgcacctata atagcccgcg
    16381 ccaatgaccg aacatgtaca tgtggacgtg agagcatggc cagacgcggg tgggaggtga
    16441 ggcggacttc cgccctcttg ttgctcccgc tcctggcggc cgtgccttgc gccggctgcc
    16501 tgaatgccgg tccggaggtt cggtccgtag aggcttctgc cgcttcgggc ggtcctgcag
    16561 agacctcgaa tgaaagcgtg gcaggtacgg ggggcatgcc cgaggccact agcatcgggg
    16621 ctccgcagat cgagggcgat ctcgctgggg ctactgacgc ctccggggtc cctttcgtcg
    16681 cgaagacgca ggcgagcggc ggatccggct acgatgccga caccgtgctc gcggtgcgct
    16741 acgaggctaa cgaggactac gagcgggcgg tgatcgatct cgggaccggc ctggagcccg
    16801 gcggtcccgt gcccgagtgg agcctcgtga gccaggaagg gaaaggcctt ctgcgcgtaa
    16861 ccctgccttc ggccagcgcg acgtgtgtat cggatggcga attcggcgat gggttgctcg
    16921 agagtttcca cgtcgtgcgc gcccccgagg gaggcatgtt catggacatc ttcgcgcgcg
    16981 aggcgttcct ctacaaggtg gtcgagctgg gggaccccgc gcgcctcgtc ttcgacttca
    17041 agccgaaggg agatctgaag gcgccgctcc ccgcggagag cggcgacacg gtcctcgtcg
    17101 agccacgctc gcacgccagc atctccgatc ccctcacagt aaacggctac tcccgcaact
    17161 ccgaagccgc gaacactatt accttgatcg attcggaagg aaaaatcctg gtgcgtaaga
    17221 ccgtcctgag caacgacaga gaccatacct gggggtactt cgaaaccacc ctggatctgc
    17281 cgccgttctc cggaaagggg accctgagag tcgggaccga aagcgcccgc aacggctcct
    17341 tcgaaggcac cgaaatcccc ctttgggccg gccagtaggc ggccagcgag cgtccagtcc
    17401 ccccaatgcg gaaagtgaag ctgacaggca gacgctgaca agcgaagctc gagcgtgctg
    17461 actggctgac tagccgagag acttcacgaa gtacccgggg tttagtggcg ctccggtggc
    17521 gcgcagtacg gtatcgcgcc agttctcgcg ggcgccgggg ccgaagaagg attcgaggag
    17581 gtagcggcct gcgacctcgt tctggtagaa ggggcctcgg gttatgtgag actccaggta
    17641 gttccttagc tgggccgaga tcaactcccc gagcatgtag ttgtgatagt agacgggggc
    17701 tacggcaacg tggatgacgg cagcccagtc gggctcgt
//
LOCUS       DASTKM010000267         7478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1328798, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000267 DASTKM010000000
VERSION     DASTKM010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328798"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VFQ10_10795"
     CDS             <1..914
                     /locus_tag="VFQ10_10795"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET9965872.1"
                     /translation="ITTDITERLILIDGIDQVEVDFGVMSHEDRQNLLSSLHGGPSEL
                     ASAFGDESKTRIIAVVSGKGGVGKSTVAVNLAAALDRAGHSVEILDADVHGSSIPVML
                     GSLEKPNVVEGVIFPIESNQGLKFISMGNFVNEGQAIIWRAPIVNKALTQLMRDVYWD
                     EPDFIIVDMPPGTGDVALTVAQMIPKAEALVVTTPQADAARVALKAGKMAVQAHLKVA
                     GVVENMAYAECPECGEQLQIFGGDGGDRVAGELGTEVMGRIPIVADATGEPGRSLFEV
                     DSSPARVFDEMAATLAASKVRRRIKVL"
     gene            complement(987..1916)
                     /gene="mdh"
                     /locus_tag="VFQ10_10800"
     CDS             complement(987..1916)
                     /gene="mdh"
                     /locus_tag="VFQ10_10800"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9965873.1"
                     /translation="MAKRKVTVVGAGNVGGTTAQRLAERNYADVVLVDIVEGLPQGKA
                     LDILESGPILGYDSNVTGANDYEETAGSDVVVITSGSPRKPGMSRDDLLKTNQNIVAS
                     VTEEVVSHSPETILVVVANPLDAMCHVAMETSRFPRERVVGMAGILDTARYRTFIAEE
                     LGVSVRDVFALVLGGHGDTMVPLPSMATVGGVAITELLPEDRVEAIVERTRQGGAEIV
                     GLLQSGSAFYAPSAAAAEMVDAILLDQKRILPCAAYLQGEYGIDDLFVGVPVKLGASG
                     VEDIIELRLTGEELRDLNDSADAVRDLVQVLHG"
     gene            2041..2610
                     /locus_tag="VFQ10_10805"
     CDS             2041..2610
                     /locus_tag="VFQ10_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HET9965874.1"
                     /translation="MTHAIQTRQSVGRVRQDPVPEDLVRRILESAVHAPNHKITEPWR
                     FHVFTGKGRGELARARAETARLQAEAEGEDQEMAAGRISRERKKAFRAPVVIAVISAS
                     GRDEVETLENYAACAAAVQNMQLTAHSLGLASIWRTGPVAYHEHMRAFFGLREGDRIV
                     AYLYVGYPDMGERPRRRSPAGERTVWHGG"
     gene            complement(2846..3646)
                     /locus_tag="VFQ10_10810"
     CDS             complement(2846..3646)
                     /locus_tag="VFQ10_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9965875.1"
                     /translation="MATGTTFDVAQMRVHILDDGVFITDAGNLFGGSRKASIKGAMHP
                     VLIEAGDNLVLIDAGFGPDLPEILVGRYELRRDKHLLDHLGEAGYSAGDVTHVILSHL
                     DADHVGWALGPPSFPKATVYVQEAALEEARQMPEKDGRRASVPAVEKGVEESWCELLD
                     TDGDILPGVKVEVRSGHSAGHQIVWIGEDDDLALYTGDLAPAKIWLDPDLISGVDTDP
                     EAARQNRIEVLSAAESRDAPVILYHEPGDCLVKVRRTDKGFEAIPIGD"
     gene            complement(3768..5006)
                     /locus_tag="VFQ10_10815"
     CDS             complement(3768..5006)
                     /locus_tag="VFQ10_10815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9965876.1"
                     /translation="MAERYDLVVVGAGPGGSSAAYHSAKAGLNTLLIDRQEFPRDKTC
                     GDGLMPHAASEVALMGLADWLDEPHHGKFTGFSIYTHTAYLRQGVPPSLHGPRGYVVK
                     RQETDAKLLERAISAGADFRSGVRATALLRSGSGTVTGLEATEAGESSRFEAPLVIVA
                     DGVGGFAGKGMKAHQNAVARRQYFRNVDGPDKQDLHVFITKDMNSHGAGYGWVFYLGD
                     GKANVGAGVSTAALARTGRNLKDFFDRFLEDPQVAAWLENATPEGPPTSWSLKMGMWG
                     AQRYGQGLMIVGDAGSMVHPISGEGVGYALESGRLAASWANEAHLREEFSASVLSGYE
                     RQLRHRRAREHFSGYALVNMMPNLMLLEPLFKACEKDPEARRTLIEAFTGDAPVYSLL
                     KHPRMLAKALRDSVTERMLP"
     gene            5073..5282
                     /locus_tag="VFQ10_10820"
     CDS             5073..5282
                     /locus_tag="VFQ10_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965877.1"
                     /translation="MVDRNRPARYEVGERAALRATGQPVEILDVRRGEFVPDFVYKVR
                     VLAQKPARPYNLSVPEHDLADLAGC"
     gene            complement(5293..6363)
                     /locus_tag="VFQ10_10825"
     CDS             complement(5293..6363)
                     /locus_tag="VFQ10_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965878.1"
                     /translation="MNREHQMTSTIETLERELGEAQTEREAEQRAVIRAENAHEAACS
                     ARDEAWSEGSATDPEVEELTDLVYRRRRELYDAIDRLKAAEGKERRIKSRLMYVLPAE
                     RRRYLSGTVDEEFEALLAAVREAYSDTLPGARVSVVTERPGEVDLRGDATWRALERLY
                     SGTVRRWVEIRSEVDSPEAGRMARQISYYAGGHTPRTFVHHEEISAGSFRGPRELRTL
                     AGYESAEGRMALARITARRYLDENSVGRLGKRPQSLVQVAEIARLYQTMSLTVDEIRW
                     RLEQLISLRFEVLEAVYKLTGQRRRFSFYEIARAARLLDGHFPDSGVAAEVLTQCVQQ
                     ALTTGVPLVDVAYMNVTTGEGF"
     gene            6533..7201
                     /locus_tag="VFQ10_10830"
     CDS             6533..7201
                     /locus_tag="VFQ10_10830"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET9965879.1"
                     /translation="MDFNTESGSSGGSGGSSGGPGGTRPRVSGGASNVEFDYSDPVQS
                     FINAVRSVVTAPADFFRGIRRQGDFVNPLIFAIICYEVSAILGGLLSLVGVSGSRGFG
                     AFLIGLILAPIGAAIGLFIGAGILHLLVMLFVGSRNSGFEGTFRVSAYSSVTSLVSWI
                     PFIGWIASLYGIYLAIMGIREVHNTTTGRAALVVLIPAVVVFLLVLLIIAAIGAALFF
                     GTQQ"
     gene            7296..>7478
                     /locus_tag="VFQ10_10835"
     CDS             7296..>7478
                     /locus_tag="VFQ10_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET9965880.1"
                     /translation="MAQTVTLIPGDGIGPDLTDSVKEVIGALGVDIEWEIAEAGETVM
                     DREGTPLPESVLDSIRR"
BASE COUNT         1326 a         2346 c         2365 g         1441 t
ORIGIN      
        1 ccatcaccac tgacatcaca gagcgcctca tattgatcga tggcatagat caagtggagg
       61 tcgacttcgg cgtgatgagc cacgaagacc gccagaacct gctcagctcc cttcatggcg
      121 gcccctcgga gctggcttca gcctttggcg atgagtcaaa gacccgcata atcgcggtgg
      181 ttagcggcaa aggaggggtt ggcaagagca ccgtcgccgt aaacctcgca gcggcgctcg
      241 acagggctgg acactccgtt gagatactcg acgcagacgt acatggctct tcgatccccg
      301 tgatgcttgg ctctttggag aagcccaacg tggttgaggg tgtgatattt cccatagagt
      361 ctaatcaagg gttgaagttc atctccatgg gcaacttcgt aaacgaaggg caggccataa
      421 tctggcgggc tcccatagtg aacaaggctc tgacccagct gatgcgcgac gtctactggg
      481 acgagcctga tttcatcatc gtggacatgc ctccagggac aggggatgtg gcccttaccg
      541 tggcgcagat gatccccaag gccgaggctc tggttgtcac aaccccccag gcagacgcgg
      601 cccgcgttgc actgaaggca ggcaagatgg ctgtgcaggc acacctgaag gtagcagggg
      661 tggtagagaa catggcttat gcggagtgcc ctgagtgcgg cgagcagtta cagatctttg
      721 ggggtgacgg aggagatagg gtagcagggg agctaggcac tgaggtgatg gggcgcatcc
      781 cgatcgtggc tgacgcgaca ggtgagccag gacgctccct gtttgaggtc gattcgagtc
      841 cggcccgggt cttcgacgag atggccgcta ccctcgccgc gagcaaagtt cgcaggcgca
      901 taaaggtcct gtagagctcg tcagctagtc agcacgctgc ggctttacgc gctttcagcc
      961 caacgcaacg ccgccttggt aggcttctag ccgtgcagga cttgcacgag atctcttacg
     1021 gcgtcggcgc tgtcgttcag atcccgcagt tcttcgcccg tgaggcggag ctcgatgatg
     1081 tcttcgacgc ctgaggcgcc gagcttgacg gggactccga cgaagaggtc gtctattccg
     1141 tactcgccct gcaggtaggc ggcgcagggg aggatgcgct tctggtcgag taggatggcg
     1201 tcgaccatct cggctgcggc tgcggaaggc gcgtagaagg cgctcccgct ctggaggagg
     1261 ccgacgatct cggccccgcc ctggcgggtg cgctcgacaa tagcctcgac cctgtcttcg
     1321 gggagaagct ccgtgatcgc gaccccgcct acggtggcca tgctcggcaa cgggaccatc
     1381 gtgtctccat gccccccgag gacgagcgcg aagacgtcgc gcacggagac cccgagctct
     1441 tcggcgatga acgtgcggta gcgggccgtg tcgaggatgc cggccatgcc gaccacccgc
     1501 tcgcggggga agcggctcgt ctccatcgcc acgtggcaca tggcgtcgag cgggtttgcg
     1561 acgaccacga ggatcgtctc aggcgagtga ctgacgacct cctccgtaac gctcgccacg
     1621 atgttctggt tcgtcttgag caggtcgtcg cggctcatcc caggcttgcg ggggctgccg
     1681 ctggttatga cgacaacgtc cgagccagcg gtctcctcgt aatcgttggc gcccgttacg
     1741 ttggagtcgt agccgaggat cggcccgctc tccaggatgt ccagcgcctt accctggggt
     1801 agtccttcca cgatgtcaac cagcacaacg tcggcatagt tgcgctctgc cagcctctgg
     1861 gctgtcgtcc ctccgacgtt gcccgcgcct actacggtga ctttcctctt cgccatctcg
     1921 ttccctcctg tgttcgactt gccgggtgag gtcatgatat gtgaatacct cagagcttgc
     1981 cgcgcctaac ccggatagaa tcagttcagt gagtgcgact ttcgaagagc ttcgttggat
     2041 gtgacccacg ccatacagac gcgccagagc gtcggccgcg tgaggcagga tcctgtgcct
     2101 gaagacctcg tcaggcgcat cctggagagc gccgtgcacg cccccaacca caagatcacc
     2161 gagccgtggc gctttcacgt gtttacggga aaggggcgtg gcgagctggc ccgggcgagg
     2221 gccgagacgg cccgcctgca ggccgaagcc gaaggggagg accaagagat ggcggcgggt
     2281 aggatcagcc gcgagcgaaa gaaggccttc agggcgcctg tggtcatcgc tgtgatttcc
     2341 gcctccggcc gtgatgaggt ggagacgctg gaaaactacg cggcctgcgc tgcggccgtc
     2401 cagaacatgc agctcacggc ccactccctc ggcctcgcga gtatctggcg caccggacct
     2461 gtcgcctatc atgagcacat gcgcgccttc ttcggtctaa gagagggaga caggatcgtc
     2521 gcctacctct acgtcggcta tcccgacatg ggcgagagac cccgcaggcg cagccccgca
     2581 ggcgagagga ccgtctggca cggcggctga aaaccattca ccgcaaccta ccggcctcac
     2641 ttacgtatta ctggtagtca accaagcgag gagcgagagt gaagacgatt ctgagacgcg
     2701 cgctcatact ggctgtgccc atcgtgtggc gcaagatcag gaaccgccgg cgcgaacgct
     2761 agtcagcaag gacctgccaa tgcgaaagct gaccagctga aatgctgaca agcgcccgaa
     2821 agggcgcttg ctggaatgct gtaactcagt ctccgatagg gatggcctcg aaccctttgt
     2881 ccgtacgacg gaccttgacg aggcagtccc ctggctcatg gtagaggatg acgggggcgt
     2941 cacgtgactc tgcggccgag agcacttcga tgcggttctg gcgggctgct tccgggtctg
     3001 tatccacccc ggagatgagg tcagggtcga gccagatctt ggcaggcgcg aggtctcctg
     3061 tgtagagtgc gaggtcatcg tcctccccga tccaaacgat ctgatgcccc gcggaatgcc
     3121 ccgaccgcac ctcgaccttg acgccaggca ggatgtctcc gtccgtgtcg agcaactcgc
     3181 accagctttc ttccacgccc ttctcgacgg cagggacgga cgccctgcgg ccgtccttct
     3241 ccggcatctg ccgcgcctcc tcgagcgcgg cctcctgcac gtagaccgtg gctttgggga
     3301 agctcggggg gccaagcgcc cagccgacgt ggtcggcatc caggtgcgag agaatgacgt
     3361 gggttacgtc cccggccgag tagcccgctt ctccgagatg gtcgagaaga tgcttatcac
     3421 gcctcagctc gtagcggccg accaggatct caggcaggtc tggcccgaac ccggcgtcga
     3481 tgagcacgag gttatctcct gcctcgatca ggacggggtg catcgcgccc ttgatgctgg
     3541 ccttccgaga gccgccgaag aggttaccag cgtctgtgat gaatacccca tcgtccagga
     3601 tgtggacccg catctgtgcc acatcgaagg ttgtcccagt agccaagtcg ccgaaccctc
     3661 ctctggtcgt gtatccgaat gatagcgggc accacgcttc gacgctcagt tctctggtgg
     3721 gcccagatgg agtcatcagc cctcgccggg gctggacgat attgtcctca aggcagcatc
     3781 cgttccgtga cgctatccct cagtgccttc gctagcatgc ggggatgttt caggaggctg
     3841 tagacgggcg cgtcgcccgt aaaagcctcg atgagcgtcc ggcgagcctc agggtccttc
     3901 tcgcaagcct tgaagagggg ctcgagcagc atcaggttcg gcatcatgtt gaccagcgcg
     3961 tatccggaga agtgctcgcg ggcccgcctg tgcctcagct ggcgttcgta gccggacagg
     4021 accgaggcgg agaactcttc ccttaagtgg gcctcgttgg cccacgaggc ggctaggcga
     4081 cccgactcca gggcgtaacc gacgccctcc ccgcttatgg ggtggaccat gctaccggcg
     4141 tctccgacta tcatcagccc ctgaccgtag cgttgcgcgc cccacatccc catcttgagc
     4201 gaccagctcg ttggcggacc ttccggcgtg gcgttctcca gccaggctgc cacctgcgga
     4261 tcttcgagaa agcggtcgaa gaagtctttg aggttccggc ccgtgcgggc aagcgctgcc
     4321 gtcgagacgc ctgccccgac gttcgccttt ccgtctccga ggtagaagac ccagccgtag
     4381 cccgcgccgt gcgaattcat gtccttcgtg ataaagacgt gcaagtcctg cttgtccggg
     4441 ccgtcgacgt ttctgaagta ctgcctgcgg gcgacggcgt tctggtgggc cttcatccct
     4501 ttgcccgcga agcctccgac gccgtcggcc acgattacca gcggtgcttc gaagcgcgag
     4561 gattcgcccg cctccgtggc ttcgaggcct gtcaccgtgc ctgaccccga cctcagtagg
     4621 gcggtggccc tgacgccgga acggaagtcg gcgccggctg agatggcacg ttcgaggagc
     4681 ttggcgtcgg tctcctggcg ctttacgaca tagccccgag gaccgtgcag ggaaggggga
     4741 acgccctgac gcaggtaggc tgtgtgcgtg tatatggaga atcctgtgaa cttgccgtgg
     4801 tgcggctcgt cgagccagtc ggccaacccc atcagtgcta cctccgaggc ggcgtgcggc
     4861 atcaagccgt cgccacaggt cttgtccctc gggaactctt gcctgtcgat gagcagtgtg
     4921 ttcagccccg ccttcgcgga gtggtaggcc gccgaggagc caccaggacc ggctccaacg
     4981 accacgagat cgtaccgctc ggccatcgtc ctccctctcg aaaccttccc gactgtgcca
     5041 gaattatact tcggctagaa acgggaggga acgtggtcga ccgcaaccgg cccgcgcgct
     5101 acgaggtagg agagcgcgcg gccttgagag ccacaggaca accggtcgaa atactcgacg
     5161 tcaggcgggg cgagttcgtc cccgactttg tctacaaggt gcgcgtcctc gcacagaagc
     5221 cagcacgacc ctacaatctc tcggtgcctg aacatgatct tgcagaccta gcagggtgct
     5281 gacgagtgaa ggctaaaagc cttcgcctgt cgtgacgttc atataggcga cgtcgaccag
     5341 tggaacgccg gtcgtgaggg cctgttgtac gcactgggtg aggacctcgg cggcgacgcc
     5401 cgagtcgggg aagtgaccgt ccaggagacg ggcggcacgg gcaatctcgt agaaagagaa
     5461 acgacgacgc tggccggtta gcttgtagac tgcctcgagg acctcgaagc gcaaggagat
     5521 cagctgctcc agtctccagc ggatctcgtc aacggtgagg ctcatggtct gatacaggcg
     5581 ggcgatctcg gcgacctgga cgaggctctg ggggcgcttg ccgaggcgtc cgacggagtt
     5641 ttcgtcgagg tagcgcctcg ccgtgatccg cgctagcgcc atccgtccct ccgcactctc
     5701 gtagccggcc agggtcctga gctcccgcgg gcccctgaag gagcctgcac ttatctcctc
     5761 gtggtgcacg aaggtgcgcg gggtatgacc gcctgcgtag taggagatct ggcgcgccat
     5821 cctccctgcc tcaggcgagt cgacctcgct ccttatctcg acccacctgc gtaccgtgcc
     5881 cgagtacaac cgctctagcg cccgccaggt ggcgtcccca cgcaggtcca cctcgccagg
     5941 tctctccgta accacggaaa cccgcgcacc gggtagagtg tcactgtatg cttccctcac
     6001 tgccgcaagc agtgcttcga actcctcgtc aacggtccca gacaggtacc tgcgacgctc
     6061 ggcagggagc acgtacatga ggcggctctt gatcctgcgc tccttgccct cagccgcctt
     6121 gagccggtct atggcatcgt agagctccct ccgccgacgg tagacgagat cggtcaactc
     6181 ctccacctcg ggatcggtcg cagaaccctc ggaccaggcc tcatcacgcg ctgagcacgc
     6241 cgcctcgtgg gcgttctcgg ccctgataac ggcccgctgc tccgcttctc gctccgtctg
     6301 ggcctcgccc agctcccgct cgagggtctc gatcgtcgag gtcatctgat gttccctgtt
     6361 cacactctac gcatgactcc acagccattt tacagcacac cgcatgcccg cggtgctccc
     6421 ggaaggcccg cgttggaacg tcaggcaact actagtttca tggttgcgcg ggttgtcgag
     6481 aacgtgtaca tttctactag ttatcctaag tgcgatggga gaggagattc acgtggattt
     6541 caacacggaa tctggcagta gcgggggatc gggcggttcg tctggggggc ctggcggaac
     6601 acggccccgg gtatccggtg gtgcttcgaa tgtagagttc gactacagcg atcctgtcca
     6661 gagcttcatc aacgcggtta ggagcgtcgt aaccgcgccc gccgactttt tccgcggcat
     6721 caggcgccag ggcgacttcg tgaacccttt gatcttcgcg atcatctgtt acgaggtttc
     6781 agcaatcctg ggcggcctgc tcagtctggt gggggtaagc ggcagccggg ggttcggcgc
     6841 tttccttatc ggcctaatcc tcgccccgat cggcgccgcc atcggcctgt tcatcggggc
     6901 cggcatactg cacctgctcg tgatgctgtt cgtaggctcg cgcaactcgg gcttcgaggg
     6961 tacgttcagg gtaagcgcct actcttccgt gaccagcctc gtaagctgga taccctttat
     7021 cgggtggata gccagcctgt acggtatcta cctggccata atggggatca gggaggttca
     7081 caacaccacc acgggtaggg ccgctctcgt ggtcctcata cccgccgtgg tggtgttcct
     7141 gctcgtcctg ttgatcatcg ccgcgatcgg agctgctctt ttcttcggca cccagcagta
     7201 gctggcgagc ctccggtacg gtccgtagtc gggaatagct gccctcacgc caggccgcta
     7261 tactgtcggc ggtctacagc gaagggggtt tgcgcgtggc gcagacggtt actctcatac
     7321 cgggcgacgg gattggcccg gatctcacgg actccgtaaa ggaggttata ggcgcgctcg
     7381 gcgtcgacat cgagtgggag atcgccgaag ccggcgagac ggtgatggat agggaaggta
     7441 cgcctcttcc cgagtcagtg ctcgactcta tccggcgc
//
LOCUS       DASTKM010000268         4353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_446261, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000268 DASTKM010000000
VERSION     DASTKM010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446261"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1818
                     /locus_tag="VFQ10_10840"
     CDS             <1..1818
                     /locus_tag="VFQ10_10840"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET9965881.1"
                     /translation="SISFLNPARDGAVLPILHLNGYKIANPTVLGRSSDDEVRDLLEG
                     HGYEVHFVEGDAPPAVHQAFAATLDACHEGIRAIQRQGRENGVSGRPRWPAIVLRTPK
                     GWTGPDEVGGLPVEGTFRAHQVPLSNVRSDPEQLAMLEEWMHSYHPEELFDEEGRLIP
                     ELAAHAPRGDRRMGVSPHANGGRVLEDLDLPDFRGYALEVEKPAAERHESTRKLGELL
                     RDTFARNARQRNFRLFCPDEINSNRLGVVFEVENRCFVGPTIPNDDHVSHDGRVFEVL
                     SEHLCEGWLEGYLLSGRHGLYATYEAFAMISSSMTVQHTKWLQEAINLPWREPVASLN
                     ILLSSTAWRNDHNGFSHQGPGLIDVMLSKRGTVARIYLPPDANSLLSVTDHCLRSRNY
                     VNLIVIDKQPQLQWLDMEAAIAHAAAGVSIWEWASNVSDEEEPDVVLGCAGDIPTLET
                     VAAAQWLRENAPELKVRVVNVVDLMTLFPREFHPHGMDETRFVELFTANKPIVFAFHG
                     YQRALHEIVHGHVDVDRFHVRGFKEQGTTTTPFDMVVLNGMSRYHLAAEVLRRSARQR
                     EDRTPTLISECEAAIARATAYSREHFEDPPEVRDWTWTL"
     gene            2085..2882
                     /locus_tag="VFQ10_10845"
     CDS             2085..2882
                     /locus_tag="VFQ10_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET9965882.1"
                     /translation="MIRAFVFDLDGTLVETEEVKALSYARAATELRPDLNEGEVVAAF
                     GDLVGLSRQEVAVGLMWRFELEDAARARMAEFDVGTPWQAYVQIRLGIYEEMLGDPKL
                     IQEHRYPHNIALLRSVRREGFPTALATQSHREQARRVLGILGLEDEFDVVVTRDDVEH
                     GKPDPEMHLLAARELDFEAGECLAIEDSPAGVQGALAAGMEVIAVTTRLTRQKFRDTD
                     LLDRSHVVDDPRTLPEVVHRLIGAAGKPPQSIYERRVREGVEEGPET"
     gene            2879..3094
                     /locus_tag="VFQ10_10850"
     CDS             2879..3094
                     /locus_tag="VFQ10_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HET9965883.1"
                     /translation="MSSVGPGENLDVALGAAMAVLPGVGLLSLVWLVGHYALAVGLAP
                     NVLALRLRGLRRGRGLQGILNPRGGNE"
     gene            3091..>4353
                     /locus_tag="VFQ10_10855"
     CDS             3091..>4353
                     /locus_tag="VFQ10_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HET9965884.1"
                     /translation="MTMQRDGTNTPAGEDRPTTNRSDEQRRASGPGGQSTMPPARSWL
                     WFALILLANVLIVRFLLPGPEAPVEVPYTFFKEEVRAGNVEAIYSQGDTVEGRFEEPV
                     TYPPKGEGGSEPGSEPSSTSETFTTVLPSFVDPGLEELLIANGVEISAEPIQEGGGLW
                     QTLLFAFGPALLLIGGYIWLYRRSQQQGGGMGGALTSVGRSRARRYDQEQDTKVTFDD
                     VAGIEEAENELVEIVDFLKDPEKYTRLGGAAPKGVLLVGAPGTGKTLLARAVAGEAGV
                     PFFSLSASEFVELFVGVGASRVRDLFKQARENAPAIIFIDELDSIGRSRGQTAIGGSS
                     EQEQTLNQILTEMDGFSSKEGVIVLAATNQPDVLDKALLRPGRFDRRVVVNLPDKVGR
                     EAILKVHTRSVPLARSVNLGELAASTPGL"
BASE COUNT          796 a         1428 c         1433 g          696 t
ORIGIN      
        1 tccatcagct tcctcaaccc tgcgcgcgac ggagcggtct tgccgattct gcacctcaac
       61 ggctacaaga ttgccaaccc caccgtgctg ggccgttcct ccgacgacga ggtccgcgac
      121 ctgctcgaag ggcacggcta cgaggtccac ttcgtcgagg gagacgctcc gccggcggtg
      181 caccaggcgt tcgctgcgac gctggacgcc tgccacgaag gcatccgggc tatccagcgc
      241 caggggcgcg agaacggcgt ctccggacgc ccgcgctggc ccgcgatagt gttgcgtacg
      301 ccgaaggggt ggacaggccc cgacgaggta ggcggcctgc ccgtcgaggg caccttccgc
      361 gcccaccagg tgccgctgtc taacgtccgg tccgacccgg agcagctcgc gatgctggag
      421 gagtggatgc attcctacca ccccgaagag ctgttcgacg aggagggtcg cctgatcccc
      481 gagcttgccg cccacgcccc gcgaggcgac cgtcgcatgg gcgtgagccc acacgccaac
      541 ggtggcaggg tcctcgaaga cctcgatctg cccgacttcc gcggctacgc gctggaagtc
      601 gagaagcccg ccgcggagcg tcacgagtcg acccgcaagc tgggcgagct tctgcgcgac
      661 accttcgccc ggaacgcccg gcagcgaaac ttccgtctct tctgcccgga cgagatcaac
      721 tccaaccgcc tcggcgtcgt cttcgaggtc gagaatcgct gctttgtcgg cccgacgata
      781 cccaacgacg accacgtctc ccacgacggt cgggtcttcg aggtcctgag cgaacatctg
      841 tgcgagggct ggctcgaggg ttacctcctg agcggacgcc acggactcta cgcaacctac
      901 gaggccttcg ccatgatctc gtcctccatg acggttcagc acaccaagtg gctccaggag
      961 gcgatcaacc tcccctggcg cgagcccgtg gcatccttga acattcttct gtcctcgacc
     1021 gcctggcgta acgaccacaa cggattctcg caccaggggc cgggcctgat cgacgtcatg
     1081 ctctctaaga gaggcaccgt cgcgcgcatc tacttgccgc ccgacgccaa cagcctcctc
     1141 tcggtgaccg accattgcct gcgcagccgc aactacgtca acctgatcgt cattgacaag
     1201 caacctcagc tgcagtggct ggacatggag gcggcgatag cgcacgctgc cgcgggcgtc
     1261 tccatctggg agtgggcgag caacgtatca gacgaggagg agcccgacgt cgtcctcggc
     1321 tgtgccggcg acatccccac tttagaaacc gtggctgcgg cccagtggct gcgcgagaac
     1381 gcgccggaac tcaaggtgcg ggtcgtcaac gtagtggacc tcatgacgct cttcccgcgt
     1441 gagttccacc cgcacggcat ggacgagacg cgcttcgttg agctcttcac cgccaacaag
     1501 ccgatagtct tcgccttcca cggctaccaa cgcgcgctcc acgagatcgt gcacggccac
     1561 gtcgacgtcg accgcttcca cgtgcgcggc ttcaaagagc agggcaccac cacgacgccc
     1621 ttcgacatgg tggtcctcaa cggcatgagc cgctaccacc tggccgcaga ggtgcttcgg
     1681 cgctctgcgc gccaacggga ggaccgcacc ccgaccctga tctccgagtg cgaggcggcg
     1741 atcgcaaggg ccaccgccta ctcgagggag cacttcgagg acccgcccga ggtacgcgac
     1801 tggacctgga ccctttgaac ccgcccacag agatctcgac aataggtcct aaagcaaggg
     1861 tccctcacgg gaccttgacc ctgccggtgg gggcatacag agtggtcaac gtgatccgcc
     1921 cgccgtgcgc cgagaggatc gtgagggcca ggaggccgct acaacttgtg atcccacttc
     1981 gagacggtgg gctccgggga agcggcttgg gaaaggagcc tgagggtgca aagatattcg
     2041 tacgtttttg aaagcgtcgg ggagacttgt agggtggagc cggcgtgatc cgcgcgtttg
     2101 tcttcgacct ggacgggacg cttgtcgaaa ccgaggaagt aaaggcgctc tcctacgctc
     2161 gcgccgccac cgaattgcgt cccgacctga acgagggaga ggtggtcgct gcctttggcg
     2221 acctggtggg tctctcgcgt caggaggtcg ccgtcggtct gatgtggcga ttcgagctcg
     2281 aggacgcggc cagggcgcgt atggcggagt tcgacgtggg cacgccgtgg caggcctacg
     2341 tccagatcag gcttggcatc tacgaggaga tgctaggcga cccgaaactg atccaagagc
     2401 accgctaccc ccacaacata gccctgctcc ggagcgtcag acgagaaggt ttcccgacgg
     2461 ccctcgccac ccagtcccac cgcgaacagg ccaggcgcgt gctcggcatc ctggggctgg
     2521 aggacgagtt cgacgtagtc gtcactcgcg acgacgtcga gcacgggaag cccgaccccg
     2581 agatgcacct ccttgcggcg cgcgagctcg acttcgaggc gggggagtgc ctggcgatcg
     2641 aagactcccc ggcgggggtg caaggggcgt tggcggccgg gatggaggtc atcgccgtta
     2701 cgaccaggct cacccgccag aagtttcgcg acaccgacct cctggatcgc agccacgtcg
     2761 tggacgaccc tcgcacgctg ccagaggttg tacatcgcct gatcggggct gcggggaagc
     2821 ctccacaaag tatctatgaa cgccgggtac gcgagggtgt cgaggaagga ccagaaacgt
     2881 gagcagcgtg gggccgggag agaacctgga tgtcgccctc ggggcggcga tggcggtcct
     2941 gccaggcgta gggctgctct cgctggtctg gctcgtcggg cactacgcgc tcgcggtcgg
     3001 cctcgcgccg aacgtgctag ccttgaggtt gcgggggctg cgaagggggc gaggactgca
     3061 aggtatcctg aacccgagag gaggcaacga atgaccatgc aacgcgacgg cacaaacacg
     3121 cccgcgggag aagaccgacc caccacgaac cggtctgatg aacagcgtcg cgcgagcgga
     3181 ccggggggac agagcaccat gccgccagcc aggagttggc tctggttcgc gctcatactg
     3241 cttgcgaacg tactaatagt caggttcctc ttgccaggcc ccgaggcccc agtcgaagta
     3301 ccctatacct tcttcaagga ggaggtccgg gcggggaacg tcgaagctat ctacagccaa
     3361 ggggataccg tcgaaggccg atttgaggag ccggtcacgt atccgcccaa aggggaggga
     3421 ggctcggagc ccgggtcgga gccttcgagc acctccgaaa ccttcactac cgtgcttccg
     3481 tccttcgtgg acccagggct cgaggagctc ttgatcgcca acggggtcga gatcagcgcc
     3541 gagccgatac aagaaggcgg tggcctttgg cagacgctac tcttcgcctt cggtccggcg
     3601 ctgctcctga tcgggggcta catctggctc tataggcgct ctcagcagca gggcggcggc
     3661 atgggcggcg ccctcacgag cgtcggtcgg agccgcgcca ggcgctacga ccaggagcag
     3721 gacacgaagg ttactttcga cgacgtcgcc ggcatcgagg aggccgagaa cgagctcgtc
     3781 gagatcgtgg acttcctcaa ggaccccgag aagtacaccc gtcttggggg agcggcaccg
     3841 aaaggcgtgc tcttggtggg agccccggga acgggcaaga cgctcctggc gcgtgccgtc
     3901 gcgggcgagg cgggggtgcc gttcttctcc ttgagcgcct ccgagttcgt cgagttgttc
     3961 gtgggggtgg gcgccagtcg cgtgcgcgac ctcttcaagc aggcccgcga gaacgccccc
     4021 gcgatcatct ttatcgacga gctcgactcc ataggacggt cccgcggcca gacggcgatc
     4081 gggggatcga gcgagcagga gcagaccctc aaccagatcc tcaccgagat ggatggtttc
     4141 tccagcaagg agggcgtgat cgttctcgcc gctaccaacc agcccgacgt gctcgacaag
     4201 gctctcctaa gacccgggcg cttcgaccgg cgcgtggtgg tgaacctccc ggacaaggtg
     4261 ggccgcgagg cgatcctcaa ggtccatacc agaagcgtgc ccctcgcccg tagcgtgaac
     4321 ctgggggagc tggcggcatc gacgcccggg ctc
//
LOCUS       DASTKM010000269         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_447784, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000269 DASTKM010000000
VERSION     DASTKM010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447784"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFQ10_10860"
     CDS             <1..322
                     /locus_tag="VFQ10_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965885.1"
                     /translation="DGSQQEEAPPAAATGGTAGRVISSEDPTVMAGGTVEGAVVAGPG
                     SSDAPTAGTEQPETQQAPRRDREEPEPFDVGAASQEAILKRAKPLLIGAGVLLVLIWL
                     LRRR"
     gene            complement(384..842)
                     /locus_tag="VFQ10_10865"
     CDS             complement(384..842)
                     /locus_tag="VFQ10_10865"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HET9965886.1"
                     /translation="MATWNSSLNQTAFSATLHCLTGCAIGEVLGIVIGTALGWSNVAT
                     IVLAIVLAFVFGYGMTILPLLRSGMALGAVLPLAFASDTLSITVMEIVDNLIIVVIPG
                     AMDAGLGSLLFWGSLAFALAVAFVAAFPVNRYLISRGRGHAVVHKHHGHH"
     gene            1116..1952
                     /locus_tag="VFQ10_10870"
     CDS             1116..1952
                     /locus_tag="VFQ10_10870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9965887.1"
                     /translation="MRYGFVIPTGDPRTVAELAREVEGAGWDGAFYWDGICVGGMDTY
                     DPWVVMAAMAMRTERVRIGAVLTPPARRRPWKLARETVALDHLSNGRLVLPVGLGALD
                     DGGFSKVGEPTARKVRAQLLDESLDILTGLWSGEPFSYTGEHFRLEEMTFLPPPVQKP
                     RIPVWVVGAWPSKKSMGRALRYDGVLAATVGGGAESPGVTPETIREIKEYVGENRDED
                     GAFDIILEGQTPGEDPRLAASMVRPFAEAGATWWIESPWTPPNEPDDLRRRIEQGPPP
                     LD"
     gene            complement(2061..2273)
                     /locus_tag="VFQ10_10875"
     CDS             complement(2061..2273)
                     /locus_tag="VFQ10_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965888.1"
                     /translation="MWSRLTRGGDGLPREKVDAIVRLIDRYLAEEASLRNLQSDKQTI
                     HPRDLPPDKREALIEEVFEILKDARR"
     gene            complement(2335..3237)
                     /locus_tag="VFQ10_10880"
     CDS             complement(2335..3237)
                     /locus_tag="VFQ10_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9965889.1"
                     /translation="MEPGRGRVESLFVATLTHISDLHFGRPAVAERLDALKEFIAELQ
                     PDAVAVSGDLTQRCTNREFSNARAYLDSLGEIAPYTVIPGNHDIRWLGAVARNLGLAG
                     LFREQAHKFKYSRYTRHISEDLSPSLEVPGVVIAGVNTAHGITRGSITRRLQDLGVIG
                     QVRHADLMRVREIFEHAAPSSARIVMMHHNPIRGELSGRHGLANTEQALHAFADLGTE
                     LILCGHDHQDAIHTIERGAHGLIISTAGTISNRIRPGRPSSFNVVEIEDHEIHITAHA
                     WRQGRGFIPSEDRAFPRRTASRQA"
     gene            complement(3273..>3709)
                     /locus_tag="VFQ10_10885"
     CDS             complement(3273..>3709)
                     /locus_tag="VFQ10_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9965890.1"
                     /translation="EPDSEETRENRTETALRVADEGVGVLVELQMERLLTPYTLQNDQ
                     ELVENVRAMIFENSPDGVVAALGAMRERPDSTPLLGEIGVPTLVVGGEEDGIITPEVV
                     GAMAAKIPGARHVTIPRTGHLSNLEAPEKFNAAVADFLQSIR"
BASE COUNT          678 a         1091 c         1236 g          704 t
ORIGIN      
        1 tgacggctct cagcaggaag aggctcctcc cgcagcggca accggtggca cggcgggcag
       61 ggtcataagc agcgaagacc cgaccgtcat ggcagggggc acggtcgaag gagccgtcgt
      121 cgcaggcccc ggctcgtcgg atgccccgac cgccgggacc gaacagcccg aaacacagca
      181 ggctccccgc cgagatcgcg aggagccgga acctttcgac gtcggtgcgg cgagccagga
      241 ggcaatcctc aaacgggcca agcccttgct cattggcgcc ggcgtgctcc ttgtcctgat
      301 ctggcttctg cgccgccgct aaacatacag ccctaaacca agagctgaca gcgcaccgtg
      361 agagctcgat cccttacaac acgctaatga tgaccgtggt gcttgtggac gacggcgtgg
      421 cctctgcccc gtgagatcag atacctgttc accggaaacg ccgcgacaaa ggcgaccgcg
      481 agcgcgaagg cgaggcttcc ccagaaaagg aggctgccga gacccgcgtc catcgcgcct
      541 gggatcacga cgatgatgag gttgtcaacg atctccatta ccgtgatcga gagcgtgtcg
      601 gaggcgaagg ccagcggtag taccgcgccg agcgccatgc cagaccgcaa cagcggcaat
      661 atcgtcatcc cgtaaccgaa gacgaaagcc agcactatgg caagcacgat ggtggcgacg
      721 ttgctccacc ccagcgcggt cccgatcact atcccaagca cctcaccaat ggcacatccc
      781 gtcaaacagt gcagcgtcgc agagaaagcc gtctggttga gtgaactgtt ccaagtggcc
      841 ataaacatcc tccctgtgcg cgtgcgtcga tagtactata tacccctatg gggtatctgt
      901 aaaggtggat agtccgaact cgtcacaccg gagccgatct cgcatctttc gatgttacag
      961 cctgcaactt ccagtttact atacccccct actctataca gagaaggtag gtgcatctta
     1021 tctccgctgt acgctgtgtt cgatagttgg agtgcgtctg ggggtccgta tactcgtttg
     1081 tggtgagatc taggcatcgg ggagaggagg tacggttgcg ttacgggttt gtgatcccta
     1141 cgggggatcc tcgcaccgtc gcggagctag cccgcgaggt ggaaggggcg ggttgggacg
     1201 gcgcttttta ctgggacggc atctgtgtcg gaggcatgga cacgtacgac ccgtgggtcg
     1261 tgatggctgc gatggcgatg cgcacggaga gggtaaggat cggggccgta ttgacgccgc
     1321 cggctcggag gaggccgtgg aagctcgccc gcgagacggt ggccctcgac catttgtcca
     1381 acgggcgtct cgtcttgccg gtaggacttg gagccctgga cgacggtggt ttctcgaagg
     1441 tgggggaacc gaccgccagg aaggttcggg cacagcttct cgacgagagt ctggacatct
     1501 tgactgggtt gtggagcggg gagccattct cctatacagg cgagcacttt cggcttgagg
     1561 agatgacctt tttgccacct cctgtccaga agccacgcat ccctgtatgg gtggtaggag
     1621 cgtggccgag caaaaagtcg atggggcggg cgctacgcta cgacggggtg cttgcggcca
     1681 cggtcggcgg gggtgccgaa tctccgggcg ttacgcccga gaccatccgc gagatcaagg
     1741 agtacgtggg ggagaacaga gacgaagatg gtgccttcga catcatattg gagggccaga
     1801 cgccaggtga agacccgagg ctggcggcct ccatggtgcg tcccttcgcg gaggcagggg
     1861 ccacctggtg gatagaatcc ccctggacgc ctcccaacga gccagacgac ctccgccgcc
     1921 gcatagaaca gggtccgccg ccgctcgact agagcagcaa gtcagcactc cgcccggctt
     1981 ttccgggcgg agtgtcagca tctggccggt cagcattgtg gacttcggct gccaggttcg
     2041 ggggtgccga cgaacctggc tcacctgcga gcatctttga ggatctcgaa gacttcttcg
     2101 atcagggcct cgcgtttatc gggggggagg tcgcgaggat ggatggtttg tttgtcggac
     2161 tggaggttgc gcaggctcgc ctcttcggcc agataccggt ctattaggcg gactatggcg
     2221 tcaaccttct ccctgggcag tccgtcgccg ccgcgggtca gtctgctcca catgtcgaac
     2281 gtcccttccg tgaaggtggg cggtgtcgcg gcagattata tcgtcggggc actttcaagc
     2341 ctgcctggag gccgtgcggc gtggaaaggc gcggtcttcc gagggtatga agcccctacc
     2401 ctggcgccag gcatgggccg ttatgtggat ctcgtggtct tctatctcca cgacgttgaa
     2461 gctcgatggt cgtccaggac ggatacggtt cgagatggtc cccgcggtcg agatgatcag
     2521 accgtgggcg cccctctcga tagtgtggat ggcgtcctgg tggtcgtgcc cgcataggat
     2581 caactcagta ccgaggtcgg cgaaggcgtg gagggcttgc tccgtattgg cgaggccgtg
     2641 gcgccctgat agctcgcccc ttatagggtt gtggtgcatc atcacgatgc gggctgagga
     2701 gggggcggcg tgctcgaaga tctcccgcac acgcatcaag tcagcgtgtc tgacctggcc
     2761 tattacgccg aggtcctgca agcggcgggt gatcgaaccc cgcgtgatgc cgtgggccgt
     2821 gtttacaccc gcaatgacca ccccgggcac ctctagggaa ggactcaggt cttccgagat
     2881 gtgccttgtg tacctggagt acttgaactt gtgggcctgc tccctgaaga gaccggcgag
     2941 gccgaggttg cgggccacgg ccccgagcca gcggatgtcg tggttgccgg ggataacggt
     3001 gtaaggtgct atctccccga gggaatccag gtaggcgcgg gcgttcgaaa actccctgtt
     3061 cgtgcagcgt tgcgtcaggt cgccggagac ggccacagcg tctggttgaa gctctgcaat
     3121 aaactctttc agggcgtcga ggcgttcggc gacagcggga cgcccgaagt gcagatccga
     3181 gatatgcgta agggtggcca cgaacagcga ttctacccga ccgcgcccag gctccaaacc
     3241 ccgtcctgcc tccggagagg taggatccct atctacctga tgctctgcag gaaatctgcg
     3301 acggcagcgt tgaacttctc gggggcttcg aggttcgaga ggtggccggt ccggggaatg
     3361 gtcacgtgtc gggcgcctgg tatctttgcg gccatggccc ccacgacctc gggggtaatg
     3421 atgccatcct cctccccgcc gacgacgagg gttgggacgc cgatctcccc aagtagcggg
     3481 gtagagtcag ggcgctcccg catggcgccg agggcggcca cgacgccgtc gggggagttc
     3541 tcgaagatca tggcccgcac gttctccacc agctcctggt cattctgcag ggtgtagggg
     3601 gtgaggaggc gttccatctg gagctccacg aggaccccaa ccccctcatc ggcgaccctg
     3661 agggccgtct cggtacggtt ctccctcgtc tcctcggagt cgggttcgg
//
LOCUS       DASTKM010000270         6699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_448006, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000270 DASTKM010000000
VERSION     DASTKM010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448006"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFQ10_10890"
     CDS             complement(<1..181)
                     /locus_tag="VFQ10_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965891.1"
                     /translation="MTLGRSRYVGGEVVLAKEGSYVIKAWGVPHAFFNAGPGTVRVME
                     ILTPGGEFEGYFDEYE"
     gene            245..727
                     /locus_tag="VFQ10_10895"
     CDS             245..727
                     /locus_tag="VFQ10_10895"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HET9965892.1"
                     /translation="MKVRELEKAAEQAGEDRQERTARELIELLNELRVILPGVQVLFA
                     FLLTVPFTQRFPDLDDLETGVFFMTLLCTAVSTALLIAPSAHHRLLWRRGVREQRLGL
                     GNRLTIAGLIFLLPAIVGVVFVITAFIFDLTAAVVVTGFLALFFVLLWFVLPLRYREG
                     "
     gene            complement(738..2435)
                     /gene="ilvD"
                     /locus_tag="VFQ10_10900"
     CDS             complement(738..2435)
                     /gene="ilvD"
                     /locus_tag="VFQ10_10900"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9965893.1"
                     /translation="METTDTRRRSRTILEGRDRAPARSYLKGMGFSDEDLSKPIVGVA
                     HSWIETMPCNYNHRHLAEKVKQGVREAGGTPMELNTISISDGITMGTQGMKTSLVSRE
                     VIADSIELVGRGHMFDAMVTIVGCDKTIPAAAMAHARLGVPGAIVYSGSITPGRFKGR
                     DVTIQEVFEGVGANAAGKMSNEDLLDLEEHACPGAGACGGQYTANTMAMILEFLGLSP
                     MGSAGPPALDGRKDEVCARAGRLVMELLERGLTPGRILTREAFENGIAAGAATGGSTN
                     LVLHLLAIAREVGVPLDIDDFDPVSSRTPIIADLKPGGRYTAVDVDRAGGSRLLGKRL
                     LDAGLIDGTQLTTTGRTLAEEVEDAEETPGQDVIVSVDEPLRPSGGLVILKGNLAPEG
                     CVVKVAGYTRLEHTGPARVFDSEEDAMNAVQAGDITENDVVVIRYEGPKGGPGMREML
                     GVTAALVGQGLGETVALLTDGRFSGATRGLMAGHVAPEAAHRGPIAALEDGDTITFDI
                     EKRTLSVDLPWEELQARLTGWHEPEPRFATGVMAKYAALVSSASEGAVTDPRLGSAQ"
     gene            2705..3223
                     /locus_tag="VFQ10_10905"
     CDS             2705..3223
                     /locus_tag="VFQ10_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9965894.1"
                     /translation="METSKISRRVINPGTSIGHVHLKVANIERALGFYRDILGFEVTQ
                     WYGDDAVFLSASGYHHHIGLNTWMSRNADPAPPHSAGLFHLAILYPERRDLALALEWL
                     LESNYPIDGASDHGVSEALYLQDPDGNGVELYRDRPREEWPRTPEGGLAMVTRPLDVE
                     GLLAELDHEETS"
     gene            3280..3498
                     /locus_tag="VFQ10_10910"
     CDS             3280..3498
                     /locus_tag="VFQ10_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HET9965895.1"
                     /translation="MGLEALGKLLIGGALVLLVLGGLLFLLGRFGLDRLPGDLVFKRG
                     DFTLYIPIGLMILLSVVGTIVLNIIFRR"
     gene            3500..4510
                     /gene="queA"
                     /locus_tag="VFQ10_10915"
     CDS             3500..4510
                     /gene="queA"
                     /locus_tag="VFQ10_10915"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET9965896.1"
                     /translation="MLSSELDYELPEDLIAQSPAEPRDASRLMVLDVRRGTIAHHTFR
                     DLPRFLLPGDALVLNETKVLPARLQAKKPTGGKVELLFLRDLGPDRGGAWEVLARPSK
                     RLRPGLGLQTGGDRLEIVESLGDGHWAVSAPDVRGLLESSGRMPLPPYIRPTPETESS
                     YQTVYARNEGSAAAPTAGFHFTERVLQAAERAGARIASITLHVGVGTFMPVRTERLEE
                     HKMHAEYYSVPREAARIVEGSSRVVAAGTTVARTLETWAATGEAEGESELFVYPGYRW
                     RSVDTLLTNFHLPRSTLLAMVMSFGGEELVREAYRAAVRERYRFYSFGDAMLLLDGGR
                     RR"
     gene            4507..5646
                     /gene="tgt"
                     /locus_tag="VFQ10_10920"
     CDS             4507..5646
                     /gene="tgt"
                     /locus_tag="VFQ10_10920"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9965897.1"
                     /translation="MSAPVSIEVHARDGEARAGVLITPRGLVETPAFMPVGTKGTVKG
                     LTPGDLRESGAGIVLGNTYHLYLRPGSDLIREAGGLHAFMGWEGPILTDSGGYQVFSL
                     AQTRRISEEGVEFASVYDGSQHVFTPELTTKIQEDLGSDVAMVLDECPPANAGREYHE
                     ESLRRTGRWARRSKEAHGRRDQALFGIVQGGLYPDLREESLGLTVEIGFDGYAVGGLS
                     VGESREEMLKTLALTAPRLPAEKPRYFMGIGDPVGILKVVALGVDMFDCVLPTRLARH
                     GAALTRDGRINLKNAKYRTDLGPLDPECACEACAGYSRAYLAHLVRENELLGHRLVTL
                     HNVRFSVDLCLRARREIAAGRFQEWSRAWISRYEGSDLARAYELE"
     gene            5659..>6699
                     /locus_tag="VFQ10_10925"
     CDS             5659..>6699
                     /locus_tag="VFQ10_10925"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965898.1"
                     /translation="MSSRRNNLIVLALVVVLLGVAAYFIFIDQPVTESTQLGLDLQGG
                     VSAQLKGSQTGGGKVTREEMEQSADLIRQRIDRLGVTEPDVRVQSENQIVVQIPGVKN
                     PEEVIRIIGSTAQLGFYQVLASDPSLTESPQVVAADEVDAIEEDIRENLKGDDAYEEG
                     KTKILFSETPSLQGDGIEVIGYIVNKEPDMTGDVLKQNGANVEFDQTTGKRIVSLELT
                     AEGGRQMGDLTQKMVNDSLASGEPARLAIALDQDIQSAPTVQEPLGQNISISNDGLPN
                     GLPEDEAKQLETVLNTGALPVNMQVISETTVGPTLGLSSLKSGLLASLVGFGLVLLLL
                     LVIYRALGIVADV"
BASE COUNT         1160 a         2079 c         2202 g         1258 t
ORIGIN      
        1 cttcgtactc gtcgaagtag ccttcgaact cgccgcctgg cgtgaggatc tccatgaccc
       61 gtacggtccc tgggcctgcg ttaaagaagg cgtggggtac gccccacgcc ttgatgacgt
      121 aggagccttc tttggcgagc acgacctcgc cgcctacata gcgcgatctt cccagagtca
      181 tgtatcctcg cgtacagtat aaaatccttg ttaacctcat gttacacgcc agggagtgaa
      241 gagcatgaag gtacgcgagt tggagaaggc tgcggagcag gcgggcgagg acaggcagga
      301 gcggactgcg cgagagctta tcgagctact gaacgagctt cgagtgatac tgcccggcgt
      361 gcaggtgctc ttcgccttcc tcctcacggt gccgtttacc cagcgtttcc cggatctgga
      421 cgacctggag accggggtct tcttcatgac gctactgtgc accgccgtct caacggcgct
      481 tctcattgcg ccctccgcgc accaccgcct cctgtggcgc aggggtgtca gggagcagcg
      541 gctggggctg ggaaaccggc tcaccatcgc cggcttgatc ttcctcctgc ctgcgatagt
      601 cggggtggtc ttcgtgatca ccgctttcat cttcgacctg accgcagcag ttgtcgttac
      661 gggctttctc gccctcttct tcgtactgct gtggttcgtc ctcccactcc gctaccgcga
      721 gggctaagac cgaatcccta ctgcgcggaa ccgagcctgg ggtcggtaac ggccccttct
      781 gaggccgaag agacgagggc cgcgtacttg gccatcactc ctgtggcgaa ccggggctcg
      841 ggctcgtgcc acccggtgag ccgcgcctgg agttcctccc agggcaggtc cacactcagg
      901 gtccttttct cgatgtcgaa ggtaatggtg tcgccgtcct ccagggctgc tatggggccg
      961 cggtgggctg cttcgggcgc cacatggccg gccatcaggc cgcgggtcgc gccggagaag
     1021 cggccgtcgg taaggagtgc tacggtctca ccgaggccct gcccgacaag ggccgcggtc
     1081 acgcccagca tctcgcgcat tccaggtccg cccttcgggc cctcgtagcg gatcacgacc
     1141 acgtcgttct cggtgatgtc tccggcctgc acggcgttca tggcgtcctc ctcggagtcg
     1201 aagacgcggg cagggcccgt gtgctccagg cgtgtgtagc ccgcgacctt gaccacgcag
     1261 ccctccggtg cgaggtttcc cttgaggatc acgagcccac ccgaaggtct cagcggctca
     1321 tcgacggaga cgataacatc ctgtcccggc gtctcctcgg cgtcctcgac ctcctcggcg
     1381 agggtgcggc ccgtggtcgt gagctgggtt ccgtctatga ggccggcgtc gagcaggcgc
     1441 ttgccgagca gacggctccc gccagcgcgg tcgacgtcga cggccgtgta gcggcccccg
     1501 ggcttgaggt cggcgatgat cggggtgcgg ctactcacgg gatcgaagtc gtcgatgtcg
     1561 agggggaccc cgacctcgcg ggctatcgcg agcaagtgca acacgaggtt agtcgagcct
     1621 ccggtcgccg cgccagcggc gataccgttc tcgaacgcct cgcgggtcag gatgcgcccc
     1681 ggtgtgaggc cgcgctccaa caactccatg accagcctcc ccgcgcgggc gcatacctcg
     1741 tccttgcggc cgtcgagggc gggtgggccc gcggagccca tcggcgagag gccgaggaac
     1801 tcgaggatca tcgccatggt gttcgccgtg tactggcctc cgcacgcgcc agcccccgga
     1861 caggcgtgct cctcgaggtc caggaggtct tcgttcgaca tctttcccgc ggcgttggcg
     1921 ccaacgccct cgaagacctc ctggatggtc acgtcgcgtc ctttgaagcg gcccggggtt
     1981 atagagccgg aatatacgat ggcgccgggc acgccgagcc tggcgtgggc catcgcggcg
     2041 gccgggatag tcttgtcgca tccgacgatc gttaccatcg cgtcgaacat gtggccgcgc
     2101 cccacaagct ctatggagtc ggcgatcacc tccctgctta cgagggaggt cttcatccct
     2161 tgcgttccca tggttatgcc gtccgagatg gagatggtgt tgagttccat aggcgtaccg
     2221 ccggcctctc tgactccctg tttcaccttc tccgccagat gacggtggtt gtaattgcaa
     2281 ggcatcgtct cgatccagga gtgggccacg cccactatgg gcttgctaag gtcctcgtcg
     2341 gagaaaccca tgcctttgag gtaggaacgg gcgggagcgc ggtcgcggcc ttcgagtatg
     2401 gtgcgactcc ttcgcctggt gtctgtagtc tccattgttc tctcctcttg gatcacatga
     2461 cggctacccg agtataggga gcggggaagc gccgtgccag cgtgtcagca cgctcaggct
     2521 tcgccttcgc ttgtcagcat tttggcttgt tggggccgaa agtgcggtcg cggcaggtgg
     2581 atatctgtag attcgctctt ggcgttctcg ctggccggcg ggtgcgacgt tcttcctgat
     2641 cgtaaacacg ttcgggttgt ggcggtagaa tcaagggtag tgagatccat gacgaggagt
     2701 ggtaatggag actagtaaga tcagcaggcg agtgataaac ccaggaacta gcatcggcca
     2761 cgttcacctg aaagtggcga acatcgagcg ggcccttggc ttctacaggg acattctcgg
     2821 cttcgaggtg acccagtggt acggtgacga cgcagtcttc ctctccgcga gtggctacca
     2881 ccatcacatc ggcctgaaca cctggatgag ccgcaacgcc gacccggccc cgcctcattc
     2941 tgcagggctc ttccacctcg ccatcctcta ccccgagcgg cgggacctcg cgctcgccct
     3001 cgagtggctc ctggaatcca actaccccat cgacggagcc tcagaccacg gcgtctccga
     3061 ggcgctgtac ctgcaggatc ccgacggcaa tggcgtcgag ctgtaccggg accgtccgcg
     3121 agaggagtgg ccgcgtaccc ccgaaggcgg gctcgcaatg gtgacaaggc ccctagacgt
     3181 tgagggcctc ctcgctgagc tcgaccacga agagacttcc tgagccaacg gccttgacgc
     3241 ccgtgtggac atcggacgcg gcgggcatag aataggatcg tgggactcga agccttagga
     3301 aagttgttga tcggtggcgc gctcgtcctg ctcgtgcttg gcggtctgct cttcttgctt
     3361 ggtcgtttcg gcttagatcg gttgcccggc gacctggtgt tcaagcgcgg cgacttcacc
     3421 ctatatatcc cgatagggct catgatcctt ctctccgtcg tcggcactat cgtcctgaac
     3481 atcatcttcc gccgctaggg tgctctcgtc cgagctcgac tacgagcttc ccgaagatct
     3541 catcgcccag agcccggcgg agccacgcga cgcctcgcgt ctgatggtcc tggacgtccg
     3601 gcgcgggacg atcgcccacc acacgttccg ggacctgcca cgtttcctgc tgcccggcga
     3661 cgcgctcgtc ctgaacgaga cgaaagtgct tcctgccagg ctccaggcca aaaagccgac
     3721 cggcggtaag gtagaactgc tcttcctccg ggatcttggg cctgatcgcg gcggagcgtg
     3781 ggaggtcctg gccaggccga gcaaacgcct caggcccgga ctcgggcttc agaccggtgg
     3841 ggatcggctg gagatagtcg agagcctcgg cgatgggcac tgggccgtct ctgcacctga
     3901 cgtaagaggc ctgctggaga gcagtggcag gatgccgctc ccaccttaca tccgtccaac
     3961 gccagagact gagagcagct accagaccgt ctacgcgcgc aacgaggggt ccgccgccgc
     4021 accaacggct ggcttccact ttacagagag ggttctgcaa gctgcggagc gggccggagc
     4081 caggatcgcc agcatcacgt tgcacgtcgg ggtagggacg ttcatgcccg tgaggacaga
     4141 gaggctcgag gagcacaaga tgcacgccga atactacagc gtgcctcggg aagcggcgcg
     4201 tattgtagag ggttccagcc gtgtcgtcgc ggctggaacg acggtcgccc gcacgctcga
     4261 gacgtgggca gctaccggcg aggcggaagg ggagtcagag ctcttcgtct atcccggcta
     4321 ccgctggcgg tccgtagaca cgttgctgac gaacttccac ctgccgcgct ccacgctcct
     4381 ggcgatggtt atgagcttcg ggggcgagga gctggtaagg gaggcgtaca gggcagcggt
     4441 gagggagcgc taccggttct actctttcgg ggacgcgatg ctccttctcg acggcggacg
     4501 gcggcggtga gcgcccccgt ctccatagag gtacatgccc gtgacggcga ggcgcgcgcg
     4561 ggcgtcttga ttacgccgcg cgggctggtg gagacgccag ccttcatgcc ggtagggacg
     4621 aagggcaccg tcaagggact cacgcctggc gacctgcggg agtcgggggc cggcatcgtg
     4681 cttgggaaca cgtatcatct ctacttgagg cctggatcgg acctcatcag agaagccgga
     4741 gggcttcacg ccttcatggg ctgggaaggt cctatcctca ccgactcggg cggttaccag
     4801 gtcttctcgc ttgcccagac gcgacgcatc tcggaggagg gggtggagtt cgcgtccgtg
     4861 tacgacggat cgcagcacgt gttcacccca gagctgacta cgaagattca ggaggatctc
     4921 ggctcggacg tggcgatggt cctcgacgag tgtccgccgg ctaatgcggg ccgcgaatac
     4981 cacgaggagt cgctgcggcg caccgggcga tgggcgcgga ggtctaagga ggcgcacggg
     5041 aggcgagatc aggccctctt cgggatcgtg cagggcgggc tttaccccga cctgagggaa
     5101 gagagcctcg ggctgacggt cgagatcggg ttcgacggct acgctgtggg gggactctcg
     5161 gtaggtgagt ccagggagga gatgctgaag acgttggcgc tcactgcgcc caggctgccc
     5221 gccgagaagc caaggtactt tatggggatc ggtgatcccg tcggcatcct gaaggtcgta
     5281 gcgctcgggg tagatatgtt cgactgcgtg ctgccgacgc gtctggcccg tcacggcgcg
     5341 gccctgaccc gggatggccg catcaacctc aagaacgcga agtaccgcac ggacttgggg
     5401 ccgctcgatc ccgagtgcgc ctgcgaagcg tgcgcgggtt acagcagggc ctatctggct
     5461 catctggtaa gggagaacga gcttctcggc cacaggctcg ttacgttgca caacgtccgc
     5521 tttagcgtag atctttgcct gagggcgcgg cgcgagatag cggcagggcg cttccaggag
     5581 tggtcgcggg cctggatctc ccgctacgaa ggttctgacc ttgcaagggc atatgagcta
     5641 gaataattct ccgatcttat gagtagtcgt agaaacaacc tgatcgtgct ggcccttgtg
     5701 gtcgtcctac tcggggtggc cgcctacttc atctttatag atcagcctgt gacggagtcg
     5761 acgcaactcg gcctcgacct ccagggcggc gtcagcgcgc agctgaaggg ctcccagaca
     5821 ggcggaggca aggtgacccg cgaggagatg gagcagtcgg ccgatcttat ccggcagcgc
     5881 atagaccgcc tcggcgtgac cgagcccgac gtcagggtgc agtcggagaa ccagatcgtg
     5941 gtccagatcc ctggcgtgaa gaaccctgaa gaagtgatcc gcattatcgg cagtacggcc
     6001 cagctcggct tctaccaggt cctggcctct gacccttcgc tgaccgagtc gccccaggtc
     6061 gtcgccgctg acgaggtgga cgctatcgaa gaagacatca gggagaacct caagggagac
     6121 gatgcctacg aggagggtaa gacgaagatc ctgttcagcg agacgccttc cctgcagggc
     6181 gatggcatcg aagtcatcgg ctacatcgtc aacaaagagc ccgacatgac gggcgatgtg
     6241 ctcaagcaga acggggctaa cgtagagttc gaccagacca ccgggaagcg catcgtgagc
     6301 ctggagctca ccgcggaagg cgggaggcag atgggggacc ttacccagaa gatggtgaac
     6361 gactcgctcg ccagcgggga gcctgcgcgg ctggccatcg cgctcgacca ggacatccag
     6421 agcgcgccta ccgtgcagga accactgggc cagaacatct ctatctccaa cgacgggctg
     6481 cccaatggtc ttcccgagga cgaggccaag cagctggaga ccgtgctcaa cactggcgcg
     6541 ctccccgtca acatgcaggt gatctccgag accacagtag gtccgacact cggcctgagt
     6601 tccctgaaga gcgggcttct ggcctcgctc gtcgggttcg ggctcgtgct tctgttgctg
     6661 ctcgtgatct acagagcgct aggcatcgtc gccgatgtg
//
LOCUS       DASTKM010000271        11241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_625967, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000271 DASTKM010000000
VERSION     DASTKM010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625967"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VFQ10_10930"
     CDS             complement(<1..1131)
                     /locus_tag="VFQ10_10930"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9965899.1"
                     /translation="MTATLFAPKLGGQLKGGGFEGSGRDSELAKDLMVEEFGLPRATL
                     AIVFESDKLSARSEEYQRAEAEALEPLRNMEQIRSVSTYADTNDKRFLSENGNKTYAL
                     VGFNVSVGETQSLVDEVRRKVRSDELTTYVTGPSAVYLDITNASAEDIRRAEKYALPV
                     ALVILVIAFGTLVAAGVPILIGGVSVLTALAALYLLAGVYDMSVYVLSIATMLGLGLG
                     IDYALLAVSRFREELENYPIPEAVSRTVATAGRSIFFSGLAVLIGLSGLLFFPFMFIR
                     SIGVAGVLVVFVSVSAALTLLPAVLGVLGHKVNALAVRRNRGAVGAGFWGRSAEAVMQ
                     HPLLVILIVGAILCALLYPVTHMKIGIPEASVLPGEYESRAGD"
     gene            complement(1432..2553)
                     /locus_tag="VFQ10_10935"
     CDS             complement(1432..2553)
                     /locus_tag="VFQ10_10935"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9965900.1"
                     /translation="MKATGLTEFGNGYHREGLLRLLDSVENDAALHLSGQLAYREAIV
                     GSLINRLLLTEAGKQRPEIFRKPLKSPIIVLGLPRSGTTFLHRLLALDPAHRAVPWWE
                     LARPVPGPDSDEWSDRRRQVFQKKLRRLQKMVPDLDSKHYTRVDTPEECIWLLANTFL
                     SPAFWALAPVYGYLDWYKNQDRLQAYNEYHQLLQVLQAADPTRRLVLKSSTHTGAVET
                     LLQTIPGALLIQTHRNPAESSASLASLFYSLHSRMTERLDVRRMTEAILSFHEHQVAR
                     NLKARDANPGVLFDVYYDRLIADPIGTVRDIYDHYGLAWSEEFAERLNYYLQHNPRGK
                     HGAHRYAPEDFGQTGAAIAERFAPYIERFELASPNGNHS"
     gene            3236..3499
                     /locus_tag="VFQ10_10940"
     CDS             3236..3499
                     /locus_tag="VFQ10_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965901.1"
                     /translation="MSGDNCPDGLIPVDPNSKRHPADEPGFWIGLVIITTDYNEGAYT
                     KVGIEWVNDTDMFTVLLRHYPNLAPALRGVANAFWPWTKVGCE"
     gene            complement(3588..3944)
                     /locus_tag="VFQ10_10945"
     CDS             complement(3588..3944)
                     /locus_tag="VFQ10_10945"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9965902.1"
                     /translation="MHEVIKRRPRGMPLGLMSGVSYEEGRNLLREGHGILFYSDGLVE
                     AHNPNREMFGSPRLHRLIPEHDEGRSLGEVLLEELYSFTGEGWEQEDDITLLTLRSSA
                     TRLGAIEETWRLDPQL"
     gene            complement(4061..5542)
                     /locus_tag="VFQ10_10950"
     CDS             complement(4061..5542)
                     /locus_tag="VFQ10_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HET9965903.1"
                     /translation="MLNGKDILALGWPAGKVIGLGLQTARDLESRGLSREDVLAELED
                     IRRDPGGALERGSEGPVAELAREWVSIGAVEASASEEELRAEPLPYHTWGEAGVDDAA
                     RLQMETALRLPVAAGGALMADAHVGYGLPIGGVLAVREAVIPWAVGLDIACRLRLSVF
                     ELSSHVLGQKKNVIKRALEEETFFGAGIRHDGHSSHEVLDDPAWGATRFLRGLKDTAR
                     VQLGTSGSGNHFVEFGAFSVPEDLEDEKARVLAPLKPGRSYLALLSHSGSRGVGNKIA
                     TRYSKIAEERRSGLEGEVKKLAWLELSSEEGQEYWLSMRLAGRFAAANHAVIHDRLAR
                     RIGAEAAFVTEHHHNFAFTEEWRGEEMIVHRKGATPAGSGDLGIVPGTMADTGYLTLG
                     LGVEDSLNSASHGAGRRMSRTRAFKELDGSAWREDLREKGITLLGGALDEAPAAYKDV
                     DGVMAAQADLVVAVGEFSPRIVRMDAGSKQNRRRRKSGTRAGR"
     gene            complement(5867..5940)
                     /locus_tag="VFQ10_10955"
     tRNA            complement(5867..5940)
                     /locus_tag="VFQ10_10955"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5905..5907),aa:Lys,seq:ctt)
     gene            complement(6010..6909)
                     /locus_tag="VFQ10_10960"
     CDS             complement(6010..6909)
                     /locus_tag="VFQ10_10960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9965904.1"
                     /translation="MGDDQLRLYRTLSRAPFPKSYVGKVFLTAFFGTHVPLLALLVYF
                     VRDRRFGLGGALRILSVTVPATLGGTAMTLWAMYSLSEPTALASKALRRYLDSGELPD
                     LPVGYTDRAGRLMTDVQYTIERLDEALRSLGELATRDHLTGTYNRRAAEERLAEDVKR
                     AERGGGALSLMLLDLDRLKPINDAYGHRAGDACVEHFAEVLGRNLRAGDWIARWGGDE
                     FVVGMWNTQDVQPTKQVLERIVEDLRESPVVLPDGEEAHLTFSGGACRWRPGDDARGL
                     VSRADEALYQAKAEGGDTIVNLD"
     gene            7115..8047
                     /locus_tag="VFQ10_10965"
     CDS             7115..8047
                     /locus_tag="VFQ10_10965"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HET9965905.1"
                     /translation="MKRDLLRDGEVGRFFRGNLHCHSNRSDGLVDPEEVVGAYREAGY
                     HFICLSDHFEAEYGWQITDTRAFRDERFTTIIGAELSSAPWEERYAYWVTAAGVPLDF
                     EVPPVDDHAGAIRRAAECGAFVIMLHPGLNNLPLAAADGLPALDTVQAVEIYNHNLAV
                     AAHPDGANGAYMLDGLLEKGYTLLVNAGDDAHFGHPSDRFGGWVEVHCDRLDPEALLR
                     SLKAGRYYSTQGPSFRELLLDGEQLRVETSGAYAISLTGSGDRWQSGQERTSEDGEPI
                     TRADFDLAPFRGSYGRVTVVDIAGRRAWSNPIWP"
     gene            complement(8212..9483)
                     /locus_tag="VFQ10_10970"
     CDS             complement(8212..9483)
                     /locus_tag="VFQ10_10970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HET9965906.1"
                     /translation="MAQKPAVHIELRGEGWAVVREGNKRATSTHPTQSEAAESGRELA
                     RRDKTEFFLHAQDGQIREHRDYREGQPSADEGIVDTVSETVGTVTGVVGGTTGVAAQA
                     TGGASQETETGATADREENKDESVADATSSGERAGRGVGEEVRGLTGAGEPLGTPEQR
                     YAEYGIYDQDGERIGPLSDLFVDENDEPEYVGVETGLPANRSVLVPAEAITVDDRLRR
                     MVVSHPRSLVETAPSLGHEDEVTPEFERRVRVHYGLDLEERAGSGALYTGAAEPTEAR
                     SAEAAPDMSGHEEDDVRVRRSEEEIRVETRQREAGEIRVRKRVHTERESLTVPKKRVE
                     VTVERVPVEGTASEVDETASAPQIGDEEIVVPVVEEEIVVEKRPVVKEEIRIRKDVVE
                     EVEVVEEDVRREEVEIDDHTGRDTGSNQDSK"
     gene            9755..10150
                     /locus_tag="VFQ10_10975"
     CDS             9755..10150
                     /locus_tag="VFQ10_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965907.1"
                     /translation="MPRDRVELETYSIDDVIRVPIVLRDEDGVAHVRAVFRRLRYAGE
                     LGPRGLDPNETLELRGNGNGQQQATVEVTRKVADEHEPGDYLCVSIQVYDVQGNVVII
                     KTPSPSRLFRIVDEGKRNDKKTEFLGWGD"
     gene            10220..10292
                     /locus_tag="VFQ10_10980"
     tRNA            10220..10292
                     /locus_tag="VFQ10_10980"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10253..10255,aa:His,seq:gtg)
     gene            complement(10444..11094)
                     /locus_tag="VFQ10_10985"
     CDS             complement(10444..11094)
                     /locus_tag="VFQ10_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9965908.1"
                     /translation="METLRILIAEDHPLFRKGMISLLSSVPEFEVIGEATTGEEAIVR
                     AADLQPDVILMDLQMPVVNGIEATRRILQESPSVRILVVTLFEDADSVFMALRAGARG
                     YVLKDSDEEEMVLSIRAVGKGEAIFSPAIATRVLAYFSTSPPPGGAPPQAFPTLTDRE
                     REILHLIAQGHPNPSIARQLSLSTKTVGNYVSNIFTKLQVADRAQAIIRAREAGLG"
BASE COUNT         2028 a         3689 c         3318 g         2206 t
ORIGIN      
        1 gtccccggcg cgagattcgt actcacctgg caggacgctc gcctcgggta tgccgatctt
       61 catgtgggtt accgggtaga gcagggcgca gagtatcgct cccacgatca ggataacaag
      121 cagcgggtgc tgcataacgg cctctgcgct cctgccccag aaacccgccc ctaccgcccc
      181 ccggttcctc cgcaccgcga gcgcgttcac cttgtgtccg aggacgccca agaccgctgg
      241 cagcagcgtc agcgctgcgg agaccgagac gaaaaccacg aggacgccgg cgacccctat
      301 agagcggata aacataaacg ggaaaaagag caatcctgaa agcccgatca aaacagccag
      361 ccccgagaag aagatggacc ttcccgccgt agccaccgtt ctggagaccg cctctggaat
      421 cggatagttc tctagctctt cccggaagcg gctcacagcc aacagcgcgt agtcgatacc
      481 gagccccagc ccgagcatag tggcgatgct cagcacgtag acggacatgt cgtagacgcc
      541 ggccagtaag tacagcgccg ccagggccgt taggacgctc acacccccga tcaggatcgg
      601 gaccccggcc gccaccagcg tcccgaaggc gatcaccagg ataaccagcg ccacagggag
      661 cgcatacttt tcggcccggc ggatatcctc ggcgctggcg ttcgttatgt ccaggtagac
      721 ggcagaaggt cctgttacgt aggttgtcag ttcgtctgag cgcaccttcc ggcgcacctc
      781 gtctaccagg ctctgagttt cccccacaga gacgttgaac ccgacaaggg cgtaggtctt
      841 gttgccgttc tctgacagga aacgcttgtc gttggtatca gcgtaggtgc ttacggaacg
      901 aatctgctcc atgttgcgga gtggttcgag cgcctcagct tcggccctct ggtactcctc
      961 gcttcttgcg gaaagcttgt cgctctcaaa gactatagcg agggtcgccc gagggagacc
     1021 gaactcttcg accatgaggt ccttggccag ctctgagtcc ctacctgaac cttcaaaccc
     1081 cccacccttt agctggccac cgagcttcgg tgcgaaaagt gtcgccgtca ccaacaccac
     1141 ggcccagaac aataggatcg gccatcgacg gcggtaagca aacgggccca ggcgcgcgaa
     1201 cgggttcagc ggagcctacc cttggcgcaa ggttccttca tgtctctata gtaaccgacg
     1261 ccccttacct catcagggag ctaagtggcc ttccatctct cacacagtct ccgcgccgtc
     1321 aatccggtca ctgccaaagc gtggtggcga tgccacttga cgatttcgtc cccgaagtgt
     1381 cgacttggct cctacggggt cggcaagtcc tcgccgaagc gtcgccctgt actagctatg
     1441 attaccattc gggctggcaa gctcgaacct ctcgatgtaa ggggcgaagc gctccgctat
     1501 tgctgctccc gtttgtccga agtcctctgg agcataccga tgtgccccgt gcttgcctct
     1561 cgggttatgc tgtaggtagt agttcaggcg ctccgcaaac tcctccgacc aggcaagacc
     1621 gtagtggtcg tagatgtctc tgacggtgcc gatgggatcg gcaatgagcc gatcgtagta
     1681 cacatcgaac aggacgccgg ggttagcgtc tctggccttt aggttgcgtg ctacctgatg
     1741 ctcgtggaag ctcagaatgg cttcggtcat cctgcgcacg tcgaggcgct cggtcatcct
     1801 gctgtgtagc gaatagaaga ggctggccag gctcgcagac gactctgccg ggttacggtg
     1861 tgtctggatc aggagggcgc cggggatcgt ttgtaacagg gtttcgacgg cgccggtgtg
     1921 ggtggaggat ttgagtacca ggcgtcgcgt cggatctgcc gcctgcaaca cctggaggag
     1981 ttgatggtat tcgttgtagg cctgcagacg gtcctggttc ttgtaccagt ccaggtagcc
     2041 gtataccggc gcgagcgccc agaaggcggg gctgaggaag gtatttgcta gcagccagat
     2101 gcactcttcg ggggtgtcta cccgggtgta gtgtttgctg tcgaggtcgg gtaccatctt
     2161 ttgaagcctg cgtagcttct tctgaaaaac ctggcggcga cgatccgacc attcgtcaga
     2221 gtcaggtccg ggcacaggac gcgccagttc ccaccacggc actgcacggt gtgcaggatc
     2281 cagagctagc agcctgtgca ggaaggtagt tcctgaacgg ggtaagccca gtacgatgat
     2341 tggagacttc agcggcttcc ggaaaatctc gggtctctgt ttgccggctt cggtcagaag
     2401 gagccggttg ataaggctgc cgacgatagc ttcccggtag gctagctgac cggagaggtg
     2461 cagtgcggcg tcgttctcta cggagtcgag tagccgtaaa aggccctctc gatgatagcc
     2521 gtttccgaat tcggtcaggc cggtcgcttt caccgccgcc tgtaccagtg cctcctcatc
     2581 caacgttgcg acaggcaggc gggcaacccc aagatgctta cctaccgtgt ttaaaacacg
     2641 aagccgcaat ggtaaagacg gccgtgactc taccacgtaa atactccctt ctattctcag
     2701 caggaattat aagctagctt tcgaaaattg tttcggacgt cgctgcgaac gggtagaaag
     2761 cccgccgacc ctaccgtgat ggcagtagtg cgaaggggag gcgccggtac aaaatcacta
     2821 ctggctacac agcggtttgc ggatgatcga ccctgcattc attctgaatt catcggttct
     2881 ggttatattc ttcacgcgga gaccaggtac gaaggcaaca gagatggagg attcggaggt
     2941 agatgtctat gggagaagga aaacgcggtc gcgcacggct ttgcccgcat cacgctcgcg
     3001 ggcatcaggc tgttcaacgg tgtcgcggcg ctgttcgtgc caacgacgct ggcccgtcgg
     3061 tagggggtcg atccggaagc caaccctgcc gcgctctacg cgctccggct attcggcgtc
     3121 cgcaccgtac tcatcggcgc ccagttgttg ctgcgagacg gcggagtgcg cgcccactcc
     3181 ctgcgtgttg ctcctgtgat ccacgccctc gacgccacgg cggctctgat cgccggtgag
     3241 cggagacaac tgccccgacg gcctgatacc ggtcgacccc aacagcaaaa ggcatccagc
     3301 cgacgagccg ggcttctgga tcgggcttgt cataatcacg accgactaca acgagggggc
     3361 ctacaccaag gtagggatag agtgggtaaa cgacaccgac atgtttaccg tgctgctgcg
     3421 ccactacccc aacctggctc ccgcgctgcg gggcgtcgcg aacgctttct ggccctggac
     3481 gaaggtgggc tgtgagtgaa ctgacgggag gcggcgtaag cttgggcgcc ttcgacatcc
     3541 caccagcgta ttcgccgcat ctgctatctc cgacttaatg actttcgtca gagttgagga
     3601 tctaatctcc acgtctcttc tatggcaccg agtcgggtcg ccgaagaccg tagcgtcagg
     3661 agggtgatgt cgtcctcctg ctcccaaccc tccccggtga aggagtagag ctcctccagg
     3721 aggacctcac ccaaagatct tccttcatca tgttctggga tgagccggtg gagtctcgga
     3781 gaaccaaaca tctcgcggtt cgggttatgg gcctctacta gcccgtcgct gtagaacagg
     3841 atgccgtggc cctctcgcag aaggttccga ccctcttcgt agctcacgcc ggacatcagg
     3901 cccaggggca ttcccctcgg tctccgcttt ataacctcgt gcacaaggct ttcgatcctt
     3961 gtgcacgacg agccatcagc ctctcagccc tcagctagcg aggagctgac cgccaaccgc
     4021 tgactagcgt agcgcctcct tgttgccata cttcctaggg ctagcggccg gcacgcgttc
     4081 ccgacttccg cctgcggcgg ttctgcttcg agccggcgtc catgcgtacg atcctgggcg
     4141 agaactcccc cacggccacc accaggtcgg cctgggcggc catcaccccg tcgacgtcct
     4201 tgtaggcggc gggcgcctcg tcgagggcgc cgccgagcag tgtgatcccc ttctctcgca
     4261 agtcctctcg ccaggcactc ccgtcgagct ccttgaaagc ccgcgtcctg ctcatacgcc
     4321 gccctgcccc gtgggaggcg ctgttcaacg agtcctcgac gccgaggccg agcgtgaggt
     4381 aacccgtgtc ggccattgtc ccaggcacga tccccaggtc tccagagccg gcgggagtgg
     4441 cccccttgcg gtggacgatc atctcctcgc cgcgccactc ctcggtgaag gcgaaattgt
     4501 gatggtgctc ggtgacgaac gctgcctcgg ccccgatcct gcgggcgagg cgatcgtgga
     4561 tcaccgcgtg gttcgccgct gcgaagcggc cagccaggcg catggagagc cagtattcct
     4621 ggccctcctc cgaagacaat tccagccacg ccagcttctt gacctcccct tccaggcccg
     4681 aacgacgctc ctcggcgatc ttcgagtagc gcgtggcgat cttgtttccc acgcccctgc
     4741 tcccggagtg ggagagaagc gccaggtacg agcgaccggg tttcagcggc gcgagaacgc
     4801 gcgccttctc gtcctccagg tcctctggta cggagaaggc gccgaactcg acgaagtggt
     4861 taccactacc tgaggttccg agctgcacac gggccgtgtc cttgaggccc cggaggaagc
     4921 gggtggcccc ccaggcgggg tcgtcgagga cctcgtggga tgagtgcccg tcgtggcgta
     4981 tgccagcgcc gaagaacgtc tcctcctcga gggcgcgctt gattacgttc ttcttctggc
     5041 cgagcacgtg ggaggagagc tcgaagacgc tcagacgaag cctgcaggcg atatccaggc
     5101 ccacggccca ggggatcacg gcctcgcgca cggcgagcac tccgccaatg ggcagcccgt
     5161 agccaacgtg agcgtcggcc ataagggcgc cgccggccgc cacaggtagc ctgagtgccg
     5221 tctccatctg aagcctggcg gcatcgtcca cgccggcctc cccccacgtg tgatagggca
     5281 ggggctcggc gcgcagctct tcctcggagg cgctggcctc gaccgccccg atgcttaccc
     5341 actcgcgggc gagctcggcc acgggaccct cggacccgcg ctccagcgcg ccgccaggat
     5401 ctcgcctgat gtcttccagt tctgcgagca catcctccct ggagaggccg cgggactcca
     5461 ggtcgcgggc ggtctgcaat ccgagcccga tcaccttccc cgctggccat ccaagcgcca
     5521 gtatgtcctt accgttcagc atctcgcgtc tccttccttt ctgcgatggt ggtgcgaaaa
     5581 ccagatagcc cagaagtgtg ccttgtgcac cgggagcaaa tatgtgttcc tgaggaaagc
     5641 tgcggatcct gggatcgaac ctccgtctca agtttgtaag acaccttgct cacccgttga
     5701 gccaatctcc cgcaagaggt tggggcgggg atcgaacccg cgacctcctg catgcgacgc
     5761 aggcgcgctc ccagcgtgag ctacatcccc aaaacgttgc taacggacgc ttagaaggca
     5821 ctatcggggg acccatcttc ggccatcgtc tgcctcctcg tactcgagag ccgggtgaga
     5881 atcgaactca catcgaacgg gggtaagaac cccgcgcctt accaatcgcg ccaccggccc
     5941 aggtgtcttg ccgctaagta gagacgtcag aatgtgcgat ttgcgaaagc tgccctttcg
     6001 caaatcgcac tagtcgaggt ttacaatggt atccccaccc tcggccttgg cctggtagag
     6061 tgcttcgtcc gccctcgaga ctagcccacg cgcgtcatca cccggcctcc accggcacgc
     6121 ccctccgctg aaggtgaggt gcgcctcttc gccgtcgggc agcactacag ggctctcgcg
     6181 caggtcctcg acgatgcgct ccagtacctg cttggtcggc tgtacatctt gcgtgttcca
     6241 catgcctacc acgaactcgt cgcctcccca ccttgcgatc cagtcccccg cgcgtaagtt
     6301 gcggcccagg acctctgcaa agtgttcgac gcaggcgtcc ccagcgcggt ggccgtacgc
     6361 atcgtttata ggcttgagtc ggtcgaggtc caacaacatc agggagagcg ctcctcctcc
     6421 gcgttctgca cgcttgacgt cttcggcaag gcgctcttcc gcggccctgc ggttgtaggt
     6481 gcccgtgagg tggtccctgg tcgccagttc cccaagggag cgcaaggctt cgtcgagccg
     6541 ctcgatagtg tactgcacgt ccgtcatgag cctcccggcc cggtccgtgt agccaacggg
     6601 tagatcgggg agctcaccgc tgtcgaggta gcgtctcagg gccttggagg ccagcgccgt
     6661 aggctcagag agcgagtaca tcgcccagag cgtcatagcc gtccctccca gcgtggctgg
     6721 caccgtgacc gacaatatgc gcagcgcacc ccccaacccg aagcgacggt cgcgcacaaa
     6781 gtagactagc aaggccagaa gcggcacgtg ggtgccgaag aacgcggtca ggaacacctt
     6841 gcccacgtag ctcttcggaa acggcgctcg ggagagagtg cggtacagcc tgagttgatc
     6901 atcgcccatg tagtctcctt acccccgtct gatcctctcg ccagagagtc aatgcgacgg
     6961 tccgggcagt gcccggcgac gggtgtgccg cattatcgta gcggaataga gttggcgctc
     7021 ttccttggta ctctcaggat accatcaagg ttaaggcgac ctacagaagg tacacgttgt
     7081 atctggcaac agggtggtgc agtagaatac tgccgtgaag agggatctac tgagggacgg
     7141 agaagtaggt cgcttctttc gcggcaacct ccactgccac tccaacagat ccgacggcct
     7201 ggtcgatccg gaagaagtcg taggcgccta tcgcgaagcc ggctaccact ttatctgcct
     7261 ctccgaccac ttcgaggccg agtacggctg gcaaattacc gacacccgcg cctttcgcga
     7321 cgaaaggttc accacgataa taggcgccga gctcagctcc gccccttggg aggagcgcta
     7381 tgcctactgg gtcacggccg caggcgttcc actggacttc gaagtgcccc ctgtggacga
     7441 ccacgcaggg gcgatccggc gggccgcaga gtgcggtgcg ttcgtgatca tgctgcaccc
     7501 cgggctcaac aacctcccgc tcgcggcggc cgacgggctt cccgcgctcg acaccgtgca
     7561 agccgtcgag atctacaacc acaacctggc cgtcgctgcc caccccgacg gggccaacgg
     7621 cgcttacatg ctggacggcc tgctggagaa gggctacacg ttgctggtga acgccgggga
     7681 cgacgcgcat ttcggccacc cctcagaccg tttcggggga tgggtggagg tccactgcga
     7741 ccgcctcgac cccgaggccc tgctgcggtc gcttaaggcc ggccgttact actccactca
     7801 gggaccttcc tttcgcgagc tgcttctgga cggcgagcag ctgcgggtgg agacgagcgg
     7861 agcgtacgcg atctcactga ccggaagcgg cgaccggtgg cagagcggcc aggagcgcac
     7921 cagcgaggac ggtgagccca tcaccagggc ggacttcgat ctcgccccct tccgtggctc
     7981 ttacggccgc gtcaccgtcg tcgatatcgc ggggagacga gcctggagca atccgatctg
     8041 gccctgactc ataccgaggg tgctctcgtc ctccaaagtg ccgttcggac ccgttgaatc
     8101 accgagacac cttctcacgc cgcgttcagg cgaacggaag ccctttccat ggcgctgtag
     8161 agccacggtc tacctggacc tgccaggtct gtacctgtcg tcctctgttt cctatttcga
     8221 gtcttggttg gagcctgtgt cgcgaccggt gtggtcgtcg atctcgacct cttccctgcg
     8281 gacgtcttcc tcgacgacct ctacttcctc caccacatcc ttgcggatgc ggatctcctc
     8341 cttgaccaca ggccgtttct ccaccacgat ctcttcctcg accacgggaa ccacgatctc
     8401 ttcgtccccg atctgcggcg cggaggcggt ttcgtccact tccgatgccg ttccttcgac
     8461 gggtacacgc tccacggtta cctcgacacg cttcttcggc acggtgaggc tctccctctc
     8521 ggtatgcacc cgtttgcgca cccttatctc tccagcctcg cgctgccgcg tctcgaccct
     8581 gatctcctcc tccgagcgcc gcaccctgac gtcgtcttcc tcatggccgg acatatcagg
     8641 cgctgcctca gcactcctcg cctccgtcgg ctcggctgcg ccggtgtaga gagcaccgga
     8701 accggccctc tcctcaaggt cgagcccgta gtgaacccgc acccgccgct cgaactcggg
     8761 cgtaacctcg tcttcgtggc ccaggctcgg cgctgtctcc accaggctcc tggggtgcga
     8821 aaccaccatg cgccgcagcc ggtcgtcgac ggtgatggcc tccgcgggga cgagcaccga
     8881 ccggttcgcg ggcaaccccg tctcgacccc cacatactcc ggctcgtcgt tctcgtcgac
     8941 gaacagatcg ctcagcggtc cgatcctctc gccgtcctgg tcatagatcc cgtattctgc
     9001 ataccgttgt tcgggtgtcc ctaacggctc cccggccccg gtcaagccgc gcacctcctc
     9061 tccaactccc cgcccggccc tctcgccgct cgaagtggcg tcagcgacgc tttcgtcctt
     9121 gttttcctcc cggtccgcgg tggcccccgt ctccgtttcc tgactcgcgc cgccagtggc
     9181 ctgcgccgcg acgcctgttg tcccgccgac cacgccggtg actgttccta ccgtctccga
     9241 cactgtgtcc acgatcccct cgtctgcgga aggttgccct tccctgtaat cgcggtgctc
     9301 cctgatctgt ccgtcctgag cgtggaggaa gaactcggtc ttgtcccgac gggcgagttc
     9361 cctgccactc tcggccgcct ccgactgggt aggatgggtt gacgtcgccc gcttgttgcc
     9421 ctcgcggacc acagcccacc cctccccccg aagctcgatg tgcacggcgg gtttttgcgc
     9481 catctcgtct actccctttc actcaggcct cacgaggctc cgacgtcaac gtgcggctgc
     9541 tacgactttg catctgttag accaacgcat agagtatagg gttacgccag actccgcacc
     9601 gatgcacttc caaaataacc tttcggatcg gtcgcccatc cagcgggtac acacccacaa
     9661 gaatgctgat gcccgttgcc tgagacttga tggagtgttt ccctgggcgt aatttgaggc
     9721 atactttctt gcgtaatctt tacaggaggt tgcaatgccg cgagacaggg tagagctcga
     9781 gacttactct atagacgatg tgattcgtgt ccccatagtg cttcgcgacg aggacggcgt
     9841 ggcccacgtc agggccgtct tccgtcgcct ccgatacgcc ggggagctcg gtccgagggg
     9901 cctagacccg aacgagaccc tcgagttgcg gggcaacggc aatggccagc agcaagccac
     9961 cgttgaggtc acacgcaaag tggccgacga gcacgaacca ggagactatc tctgcgtctc
    10021 catccaggtc tacgacgtac agggaaacgt ggtgatcatc aagaccccaa gtccctccag
    10081 gctcttccgc atagtcgacg aaggaaagcg caacgacaag aagaccgagt tcctcggctg
    10141 gggagactga acacacctga aggcgccctg catcacgttt ccgacgcgcc ctttcgctgg
    10201 tagaatcccc ctgcgcgtgg tggccgtagc tcagttggta gagcatcgga ttgtggctcc
    10261 gaaggtcgcg ggttcgagtc ccgtcggtca cccccgattt gcaggtaaaa cgtggagtcc
    10321 agcgtctttg gccgagcctc gtttactgca acccgctaac caaggcttcg tctcgttggt
    10381 caccatcgcc taaccccgca taactactga gaccgaccga gcgaccatcg gctctgttct
    10441 gtcttatccc agacccgctt ccctagcgcg gataatagcc tgcgcccggt cggcaacctg
    10501 cagcttggtg aagatgttag agacgtagtt gccaacggtc ttcgtgctca gcgacagttg
    10561 cctggcgatc gaagggttgg gatgcccttg ggcgatgagg tgcagaatct cgcgctcgcg
    10621 atcggtgagc gtggggaagg cctgcggtgg ggctcctccc ggcgggggag aggtggagaa
    10681 gtaggctagc actcgggttg cgatagcagg gctgaagatg gcttcgccct tgccaaccgc
    10741 ccggatcgag agcaccatct cctcctcgtc tgagtccttg aggacgtatc cacgcgcccc
    10801 ggccctcaag gccataaaca ccgagtcagc atcctcgaag agcgttacca caaggatgcg
    10861 tacgctcggg ctctcctgga ggattcttcg cgtggcctct atgccgttta ccacaggcat
    10921 ctgcaggtcc atcaggatca cgtcgggctg cagatcggcg gcacgcacga tggcctcctc
    10981 gcctgttgta gcctcgccga tcacctcgaa ctcggggacc gatgagagca gggagatcat
    11041 gcccttcctg aaaagaggat ggtcttctgc gatgaggatg cgtagagttt ccaatctgct
    11101 cactccttcg ataagggcag acggaccagc acctgggtac caccttttgg ggcaggttcg
    11161 acgacgcagc ttccgcccag ctcggccgcc cgctcgcgca tcgaggggag gcccacgccc
    11221 gcactacgtt ctgcaaggat g
//
LOCUS       DASTKM010000272         9901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_980916, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000272 DASTKM010000000
VERSION     DASTKM010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980916"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VFQ10_10990"
     CDS             <1..1000
                     /locus_tag="VFQ10_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET9965909.1"
                     /translation="DDPDTGAFAYVVDLHSDRAERGHAWAAQRVWASTDKPFAVVCGV
                     TSAIQESAAARLRSSGVPVLEDVTSGLRAFQHLFERRDASTLPSLTPPDPVPRETVRR
                     WREALAAGEGLGEARALEMLADYGVPVARTVAATDLEGILAAARRLGYPVALKTVAAS
                     HKTELGGVTLGIRDEDALEAAYSEMSSRLGPAVTVQEMVEPGIEMALGVAHDPQFGPL
                     VMVAAGGVLIERLGDRRLALPPLDEVRAAKLIDRLDARPVLDGVRGAPPADVNALARA
                     LSRLSLLTRDLGDLIGALDVNPVIVGPRGCVAVDALVEPMGEHFERDARKTRPLAL"
     gene            complement(997..2184)
                     /locus_tag="VFQ10_10995"
     CDS             complement(997..2184)
                     /locus_tag="VFQ10_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET9965910.1"
                     /translation="MTPIDRELLASSLVSEGQMNLPAEAYTEAAVLDWEKEHFFDGSW
                     VCVGRSEDLRGVGDRCAVALGHESALLTRDEGGDLRAFYNVCRHRGHVLAEVGESSSG
                     RFIRCPYHAWAYGLDGSLQGAPGFGRLPGFERADYSLVPVRVAEWEGWLFANASGDAP
                     PFEEHVGDLGEMIGNHRCGELVTAARQSYEVAANWKILVENYHECYHCSSIHPELCKV
                     SPPKSGEDHEARGAWIGGSMELEDHAETMSLTGESFAPMLPHLTALQEREVYYYQLFP
                     NLLMSLHPDYVMTHRLRPIAPDRTAVECEWLFSKEAVETEGFDPSYASEFWDITNGQD
                     WGACEGVQRGASSRGYRQGPLSPDESTSGKFVAMIARGYLEGGVTQIPDWIPPERNSE
                     PLG"
     gene            2542..3720
                     /locus_tag="VFQ10_11000"
     CDS             2542..3720
                     /locus_tag="VFQ10_11000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9965911.1"
                     /translation="MTVYDVVVVGGGNLGLWTAHRLARRGFGRIAVLERGWAGGGATS
                     RSAGVVRQQGGSETAAKLGRLSRELYLRLGEELGLDSGFRETGYYIVAETEEEKEGFL
                     RLVEVRRAAGVENEWVEPEEGRRRFPDLNWARFVGATYTADDGYVHPPVAARNATFAV
                     LGDAAVDLFEMCEVLGVEVRGGRYVIESARGTFEAERVVDAGGPRGARDIGGMVGVEV
                     PVMAVRHQIVSFPYVPEGAEHPFPLVFNVGKGYYWRPEEQGVLLGMSNPVDEADETGR
                     YQIDFDWDYYEGLRHDWESAHPALGGLPVSRAWSASIDYTPDHLPIIDEPVEGFYVLA
                     AGGHGMMWGPALGEKMAGLISEGRVEDLPQEDIELARFSRKRDPNRVEDAIALPFPKA
                     "
     gene            3737..4915
                     /locus_tag="VFQ10_11005"
     CDS             3737..4915
                     /locus_tag="VFQ10_11005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9965912.1"
                     /translation="MTKAPQMMAEIPEEVKALKARIAEFVREELHPFEAEIAEHGEID
                     EEKLRALRNKARQAGFSNLNMPTEYGGMDLSMVSLVALEEESGKVTNGLGYMVADRGP
                     RELLELASPEQIERYVMPVLRGETREAWAITESEAGSDIKDIKTTAKRDGDGWVLNGD
                     KWFVTGGERAGFFIVLAWAGEEQASFLVERERPGLEFVRTPAFMHDPYASKHVELRLK
                     ECRVPEGACLGSGGDEGTKRWFSLERLMIAARCCGAAERLIDLARSWSLERSASGKRI
                     SEYQAIQFMLADSATELNAARLMVHHVAGVWDLDAPSILHGKTAMAKLFASEMAGRVA
                     DRAVQIFGGRGYMTENPAERYYRELRVDRIWEGTSEIQRSIIARGLLKRGREAYTMGG
                     "
     gene            4932..6380
                     /locus_tag="VFQ10_11010"
     CDS             4932..6380
                     /locus_tag="VFQ10_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HET9965913.1"
                     /translation="MSITVDRKKIFVGGEFFEGAKGEWRGILNPATGEVIAEVPECTR
                     EDVDEAVRAARSAFEGWFETTPAERSEMLHGLADMLEEHAEELAQAESRNVGKPIASA
                     REELPYIVDNLRFFAGAGRLLEGKSAGEYTRGYTSMIRREPVGVVGQIAPWNYPLMMA
                     VWKIAPALAAGNTVVLKPSEQTPLTTLKMAELAADIFPGGVLNVITGDGELVGAGIVK
                     HPGVDMISLTGDIATGKAVVRAAAETLKRVHLELGGKAPVVIFDDADLEAAVDSIRYA
                     GYANSGQDCTAASRVIAGPGIYDELASELSSAVSNIRTADPAEGQGVEMGPLVSADHM
                     DRVTGFLDRAKGEPGVEFLAGGNGPGGGRGFFVEPTLVTGVAQGSEIVQREVFGPVVT
                     VQRFGDEEEAIRWANDVPYGLAASVWTRDVGRALRAARKLRFGTVWINDHITTVSEMP
                     HGGFRESGYGKDMSAYAIEDYTLIKHVMTRLD"
     gene            complement(6391..7353)
                     /locus_tag="VFQ10_11015"
     CDS             complement(6391..7353)
                     /locus_tag="VFQ10_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET9965914.1"
                     /translation="MYGYRPTTRLLSMLELLQARGRVGGPELARRLEVGERTVRRYVA
                     MLQEMGVPVEAERGRYGAYTLRPGFKLPPMMFTDDEALALALGLLSARRLGLSGAAPA
                     VEGAQAKLERAMPEGLRERVRTFEEVVVPAAAAPARLPAGEVVVTLSAAVRERKRVRM
                     RYRSGESGETRRDVDPYAVVQGDAFWYTFGYCHLREGRRLFRLDRVLSVERLDESFQL
                     PPGFDTPQGVLEALADMREDRWSVEVLLETEAGEARAQLPKMGVTLQETPEGVVMRSS
                     TSDLAWMARVLAALSFPFVVRTPPELRESLRSLAAEIAALAERT"
     gene            7457..7678
                     /locus_tag="VFQ10_11020"
     CDS             7457..7678
                     /locus_tag="VFQ10_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965915.1"
                     /translation="MNDWLSVELARERNARILAEAEERRLARLAPSRSVRTALRARVA
                     RRMFAFAVALEKEETWRAVWERLEAPRHP"
     gene            complement(7805..8674)
                     /locus_tag="VFQ10_11025"
     CDS             complement(7805..8674)
                     /locus_tag="VFQ10_11025"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET9965916.1"
                     /translation="MREIEGVYVANVTPFRDDGSFAVDEDAYMDHVAWLAEMGVRGIV
                     PFGTNGEGPSVTLGEKLRVLEALFGRGFQVQVIPAVMQNNLPETLEMLRAIEDYPATA
                     VLVLPPYYFKPVEPEGMMRFYEPVLEVTGHTTIVYHIPKYAVPVPDRLVRELPVWGVK
                     DSGGEEGYAEAVLQGGKGVLLGTEDDLWRRLGTGAQGIISALANFIPEEVLALYETHR
                     EGDETGGMTLSEKLQKARTMTKEYSSPAVLKKLAEARHGAPMGTVRPPFVPAPADYDP
                     TPALQAGSRRSQT"
     gene            complement(8721..9671)
                     /locus_tag="VFQ10_11030"
     CDS             complement(8721..9671)
                     /locus_tag="VFQ10_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET9965917.1"
                     /translation="MAENLDVITAGETMVLGVPPRPGRLRHATSLELKIGGAESNLAI
                     ALSRLGFSAGWAGYLGGDEPGQLVLDRIRAEGVDTSQVRRIKERPTGLYIREQIGTDV
                     RVYYYRLGSAASTMHTDAFDPDYLGGAKFVHLTGITPALSEECRAFVLWAAREACASG
                     ARVSFDVNYRSKLWETEEAREFVEEILPDVYLLFAGDEEARALWARDDEALVRELARE
                     GPEEVVLKRGSAGSLALIDGKALEHQAFTVAQVDPVGAGDSFAAGYLAGHLWNLPAED
                     RLRVANAMGALSVATLGDYEGLPDEEELRAFIEGEESLGR"
BASE COUNT         1741 a         3085 c         3387 g         1688 t
ORIGIN      
        1 cgatgacccc gacacggggg cgttcgccta tgttgtggac ctgcacagcg accgcgccga
       61 gcggggtcac gcctgggccg cccagcgggt ctgggcctcg accgacaaac cgttcgcggt
      121 ggtctgtggc gtaacgagcg cgatccagga gagcgccgca gcgcgcctgc gctcctccgg
      181 tgtcccggtg ctggaggatg tcacctcggg actgcgagcc tttcagcacc tcttcgagcg
      241 gcgtgacgcc agtacgctgc cctcccttac accccctgat ccggtgccgc gggagaccgt
      301 acgacgctgg cgcgaagccc tggccgcggg ggaaggccta ggagaggcgc gcgcgctgga
      361 gatgctcgca gactacgggg tgcctgtggc gcgtaccgta gcagcgaccg acctcgaagg
      421 gatcctggcc gcagccaggc gcctcggcta cccggtcgcc ttgaagacgg ttgcagcttc
      481 ccacaagact gagttgggcg gggttacgct cggcatccga gacgaagacg cccttgaggc
      541 ggcttacagc gagatgtcct cgcgcctcgg ccccgcagtc acggtacagg agatggtgga
      601 gcccggcatc gagatggcgc tcggggtggc gcatgacccc cagttcggcc cgctggtgat
      661 ggtcgccgca gggggcgtcc tgatcgagag gctcggcgac aggcgtctcg ccctgccgcc
      721 cctggacgag gtaagggccg cgaagctcat cgacaggctc gacgcccgtc ccgtgctcga
      781 cggggtcagg ggcgcaccac cggcggatgt gaacgcgcta gccagggctt tatcgcggct
      841 ctccctgctg acccgagatc tcggggacct cataggcgcc ctcgacgtca acccggtcat
      901 cgtcggcccg cgcgggtgcg tggcggtaga cgccctcgtc gaacctatgg gcgaacactt
      961 cgagcgcgac gctcggaaga cgcgaccgtt ggcactctag cctaaaggct cggagttacg
     1021 ctctggcggg atccagtccg gtatctgcgt aaccccgccc tcgagatatc ctctcgcgat
     1081 catggcaacg aacttgccgg acgtcgactc atccggtgag agcggcccct gccgataacc
     1141 gcgcgaagaa gcgcccctct gtacgccctc gcaagctccc caatcctgcc cattggtgat
     1201 gtcccagaac tcgcttgcgt acgaaggatc gaacccctcc gtctcgaccg cctctttcga
     1261 aaagagccac tcgcactcca cagcggtcct gtcgggggcg atgggcctca ggcggtgggt
     1321 catcacgtag tcaggatgga ggctcatgag caggttcggg aagagctggt aatagtacac
     1381 ttcccgctcc tggagcgccg tcaggtgcgg cagcatcggg gcgaagctct cgccggttag
     1441 cgacatggtc tcggcgtgat cctcgagctc catggagccg ccgatccagg ccccccgcgc
     1501 ctcgtggtcc tcgccgctct tgggtgggct caccttgcag agctcaggat ggatggagga
     1561 gcagtggtag cactcgtggt agttctctac caggatcttc cagttcgccg ccacctcgta
     1621 actctgcctg gcggccgtca caagctcgcc gcagcggtgg ttcccgatca tctcccccag
     1681 gtcgcccaca tgctcctcga aaggcggcgc gtccccgctc gcgttcgcga acagccatcc
     1741 ctcccactcc gcgaccctga caggtacgag cgagtagtcg gccctctcga accctggaag
     1801 ccggccgaaa ccaggagccc cctgcaaact cccgtccagc ccgtaagccc aggcatggta
     1861 agggcagcgg atgaacctgc cgctcgacga ctcgcctacc tcggcgagca cgtgcccacg
     1921 gtggcggcac acgttgtaga aggcccgcag gtctcccccc tcgtcccgcg tgagtagcgc
     1981 ggactcgtgg ccgagcgcaa cagcacaccg gtccccaaca ccgcgcaagt cctcagagcg
     2041 ccccacacag acccagctcc cgtcgaagaa gtgctccttc tcccaatcga gcaccgctgc
     2101 ctcagtgtaa gcctcggcag gcaggttcat ctgcccctca gagaccaacg atgaagccaa
     2161 caactcgcgg tcgataggcg tcatgagcac tcccctcgca aacccgtccg ttatacgctt
     2221 ctcgacagcc tcgctaccag catgcggacc agcagcatgc cggttgcccc agaccgccgg
     2281 cttcagtata atctattttg aatgacggtt caagaatgca tccgggttgg attatggtct
     2341 ccagtccgtc gtggcacgag ctgctgggca tgttccggag agcccgaaag tggaaggtgc
     2401 cgcgtgcggg ctgtcgcggc gacgaggccg ccacgacggc gttttactga actgcggttc
     2461 aagagtggtg ttcgggttgc taggcacccg tggatgggac tgccgtaggg acgggcgaag
     2521 gatggtgagg aggtgttggg catgacggtc tacgacgtcg ttgtggttgg cggtggaaac
     2581 cttgggttgt ggacggcgca ccggctggca cgtcgtggtt tcgggcggat cgcggtgtta
     2641 gagcggggtt gggccggggg cggggcgacg agccgctcgg ccggcgtggt gcgccaacag
     2701 ggtggttccg agacggctgc gaagctgggc aggctgtcca gggagttgta tttgcggctc
     2761 ggggaagagt taggcctcga ctccggtttc cgggagacgg gatactacat agtcgcggag
     2821 acggaggagg agaaagaagg tttcctacgg cttgtcgagg tgcggcgggc ggcgggggtc
     2881 gagaacgagt gggtggagcc cgaggagggc agacggcgct tccccgacct gaactgggcc
     2941 cgctttgtgg gggcgacgta caccgcggac gacgggtacg tccatccgcc tgtggcggcc
     3001 cgcaatgcta cctttgcggt gcttggcgat gccgccgtag acctattcga aatgtgcgag
     3061 gtgcttggcg tcgaggtgcg gggcgggcgg tacgtgatcg agagtgcgcg cggcaccttc
     3121 gaggcggagc gggttgtgga cgctggcggg ccgcgggggg cgcgggatat cggcggcatg
     3181 gtcggggtgg aggttcccgt gatggctgtg cggcaccaga tcgtcagttt cccctacgtg
     3241 cctgaggggg ccgagcatcc gttccctttg gtgttcaacg tgggcaaggg ttactactgg
     3301 cggcccgaag agcagggtgt tctgcttggc atgagcaacc ccgttgacga ggctgacgag
     3361 acggggcgct accagatcga cttcgactgg gactactacg aggggttgcg ccatgactgg
     3421 gagtcggcac acccggcgct cggaggcttg cctgtatcgc gggcgtggtc ggcgtccatc
     3481 gactacacgc cggaccacct ccccattatc gacgagccgg tagaggggtt ctacgtcctg
     3541 gccgcggggg gacacgggat gatgtggggg ccggcgctcg gggagaagat ggccgggctc
     3601 atctcggaag gcagggtcga agacctgccc caggaggaca tagagcttgc gcggttctcg
     3661 cggaagaggg acccgaacag ggtggaggac gccatcgccc tgccgttccc caaagcgtag
     3721 aagaggagat agagctgtga cgaaggcgcc gcagatgatg gccgagatcc cggaagaagt
     3781 aaaggcgttg aaggcccgca tcgccgagtt cgtgcgggag gagcttcacc ctttcgaggc
     3841 ggagatcgcc gagcacgggg agatcgacga ggagaagctg cgggcgctgc gcaacaaggc
     3901 acgccaggcc gggttcagca acctcaacat gccaacggag tacggtggga tggacctttc
     3961 catggtctcg ctcgtggcgc tggaggagga gtcagggaag gtgaccaacg gcctcggata
     4021 catggtcgcc gacaggggac cgcgggagct cctggagctg gcaagtcccg agcagataga
     4081 gcgctacgtc atgcccgtac tgcggggcga gacgcgggag gcctgggcca tcaccgagtc
     4141 cgaggccggc tctgacataa aagacatcaa gaccaccgcc aaaagagacg gggacgggtg
     4201 ggttctgaac ggggacaagt ggttcgtgac cggcggtgag cgggccggct tctttatcgt
     4261 gctggcgtgg gctggcgagg agcaggcgtc attcctcgtg gagcgcgaga ggccagggct
     4321 ggagttcgtg cgcacgccag ccttcatgca cgacccttac gcctccaagc acgtcgagct
     4381 caggctgaag gagtgccggg tccctgaggg cgcgtgcctc ggaagcgggg gggatgaggg
     4441 gaccaaacgc tggttctcgc tggagaggct gatgatcgcg gcccgctgtt gcggggcggc
     4501 cgaaaggctc atagatctgg cccgctcgtg gtcgctggag cgctcggcgt ctggcaagag
     4561 gatctccgag taccaggcga ttcaattcat gctggccgac tcagcgaccg agctgaacgc
     4621 cgccaggctg atggtgcacc acgtggccgg agtgtgggat ctggacgcgc ccagcatcct
     4681 gcacgggaag acggcgatgg cgaagctgtt cgcttccgag atggcgggcc gtgtggctga
     4741 ccgggcggtc cagatcttcg gtggccgcgg gtacatgacc gagaaccctg ccgagaggta
     4801 ctaccgcgag ctcagggtcg accgcatctg ggagggcacg agcgagatac agcgttcgat
     4861 catcgcccgc gggttgctga agcggggccg agaagcgtac acgatgggcg ggtagcgaga
     4921 aggagagagc cgtgagcata actgtggaca gaaagaagat cttcgtcggt ggggagttct
     4981 tcgaaggggc gaaaggggag tggcggggga tcctgaaccc ggcgacaggg gaggtcatcg
     5041 ccgaggtacc agaatgcacg agggaggacg tggacgaggc ggtcagggcg gcgagaagcg
     5101 ctttcgaggg ctggttcgaa acgacgccgg ccgagcggtc cgagatgctc cacgggctcg
     5161 ccgatatgct ggaggaacac gccgaagagc tcgcccaggc cgaatcccgg aacgtcggaa
     5221 agccaatcgc atccgcgcgc gaggagttac cttacatcgt tgataatctg cgcttcttcg
     5281 ccggggctgg gcgcctcctc gaaggcaagt ccgccggcga gtatacgcgc ggatacacaa
     5341 gcatgatccg tcgcgagccc gtgggcgtcg taggtcagat agccccatgg aactaccccc
     5401 tgatgatggc cgtctggaag atagccccgg cgctcgcggc cggcaacacc gtcgttctca
     5461 agccttcgga gcagacgccg cttacgaccc taaagatggc cgagctggct gccgacatct
     5521 ttcccggcgg cgtcctcaac gtcattacgg gggacggtga gctggtcggc gccggtatcg
     5581 tgaagcatcc cggcgtggac atgatctcgt tgaccggcga catcgccaca ggcaaggccg
     5641 tagtcagggc cgccgcggag acactcaagc gcgtacacct cgagctcggc ggcaaggctc
     5701 ctgtcgtgat cttcgacgac gcggacctcg aagccgccgt ggacagcatc aggtacgcag
     5761 gctacgccaa ctccggacag gactgcacgg ccgcctcgcg cgtcatcgcg ggaccaggga
     5821 tctacgacga actcgcctcc gagctgtcga gcgcggtctc gaacataagg accgccgacc
     5881 ctgccgaggg ccagggcgtg gagatgggcc cgctcgtctc cgcggaccac atggatcggg
     5941 taacgggttt cctagaccgg gcgaaaggcg agccaggggt ggagttcctg gccggaggca
     6001 acggccctgg cggtggtcgg ggcttcttcg tcgaaccgac gctcgtgacc ggtgtcgccc
     6061 agggctctga gatcgtgcag cgggaggtct tcgggcccgt cgttaccgtg cagcggttcg
     6121 gcgacgagga ggaggccata aggtgggcca acgacgtgcc ctacgggctt gcggcctcgg
     6181 tgtggaccag ggacgtcggc cgggccctaa gagcagcccg caagctgcgc ttcgggaccg
     6241 tctggatcaa cgaccacatc accacggtct ccgagatgcc gcacggcggc tttcgggagt
     6301 cgggctacgg caaggacatg agcgcctacg cgatcgaaga ctacaccctc atcaagcacg
     6361 ttatgacgag gttggactga acggccaggg ttaggtacgc tccgcgagcg cagcgatctc
     6421 ggcggccagg ctccgtagcg actcgcgaag ttcgggcggg gtgcgcacca caaaggggaa
     6481 tgagagtgct gccagcaccc gcgccatcca cgcgaggtcg gaggtcgaag agcgcataac
     6541 gacaccctcg ggtgtctcct gtagcgtgac gcccatcttc gggagttgtg cgcgcgcctc
     6601 ccccgcctcg gtctcgagca acacctctac ggaccagcgg tcttcgcgca tatccgcaag
     6661 cgcctccaac actccctggg gtgtatcgaa gcctggcgga agctggaacg actcgtcgag
     6721 cctctccacc gagagtaccc tgtccaacct gaacaggcgc cttccttccc gcaggtgaca
     6781 gtaaccgaag gtgtaccaga aggcatcccc ctgaacgacg gcgtagggat ccacatccct
     6841 acgcgtctca cccgattccc ctgaccggta tcgcatcctt acccgcttgc gctcccgcac
     6901 ggccgcgctc aaggttacga cgacctcgcc cgcggggaga cgcgcagggg ctgcggcagc
     6961 agggacaacg acctcctcga aggtacgcac ccgctcgcgc aggccctcgg gcatggcacg
     7021 ctccagctta gcctgggcgc cctctacggc aggggcggcg cccgatagtc cgagcctgcg
     7081 ggccgagagc agcccgaggg ctagcgcgag cgcctcatcg tccgtgaaca tcatcggtgg
     7141 cagcttgaag ccgggtctta gcgtataggc accgtacctg cccctctcgg cctcgactgg
     7201 cacgcccatc tcctggagca tcgccacgta gcggcgcacg gtcctctcgc cgacctcgag
     7261 cctacgggcc agctcggggc cacccaccct cccccgcgcc tgtagtaact ccagcatcga
     7321 gagcaacctc gtcgtcggac ggtagccgta catcctgtta cctccgaaaa actcgctgtt
     7381 tcataggtta tcaggaagga ttatgacctc tttcgcaagt actctcatca ggcaagcgag
     7441 agaggagata aaggtcgtga acgattggtt atctgtggag ctcgcgaggg agaggaacgc
     7501 aaggatactg gccgaagccg aggagcggcg gcttgcgcga ctggccccca gcaggtcggt
     7561 ccggaccgcg ctgcgggcgc gggtggcgag gaggatgttc gcgtttgcgg tggcgctgga
     7621 aaaggaagag acctggcggg ccgtgtggga gaggctggag gcgccgcgac acccgtgaca
     7681 gcggtttccg gaagggtgtc cttccggaaa ccgcgctggt cagctagtca gcagttcagc
     7741 cagtctgttt tttgtttgct cgtgactagt cgtgcgtaag gctcgggcag taggcgcggc
     7801 gctgctacgt ctgagatctt cgactgccgg cttgtagggc gggagtggga tcatagtccg
     7861 caggggcggg tacgaaggga ggtcggacgg tccccatcgg cgcgccgtgc ctggcctctg
     7921 cgagcttctt gaggaccgcg ggtgaggagt actccttggt catcgtccga gccttctgga
     7981 gtttttctga gagggtcatg cctccggtct catcgccttc ccgatgcgtt tcgtacagtg
     8041 cgagcacctc ttcggggata aagttggcga gggcggagat aataccctgg gcccccgtac
     8101 cgagcctccg ccacaggtcg tcctccgtgc cgaggagcac gcccttgccc ccttggagaa
     8161 cggcctcggc atagccttcc tccccgcccg agtccttgac gccccagaca gggagttctc
     8221 tcacgagccg gtcgggaacg gggacggcgt actttgggat gtggtagacg atggtggtat
     8281 gacccgtcac ctcgagcacg ggctcgtaga agcgcatcat cccttccggc tccacgggct
     8341 tgaagtagta gggagggagg acgagcacgg cggtagcggg gtagtcctct atagcgcgca
     8401 gcatctccag ggtctcgggc aggttgttct gcatgacggc ggggatgacc tggacctgga
     8461 acccgcgccc gaacagcgcc tcgagaactc tcaacttctc accgagcgtc accgaaggtc
     8521 cctcgccgtt ggtgccgaag ggcacgatgc cccgcacgcc catctcggcc agccaggcga
     8581 cgtggtccat gtaagcatcc tcgtcgacag cgaaggaacc gtcgtccctg aacggcgtca
     8641 cgtttgcgac gtacacgcct tctatctctc gcatcctcgt acctcccata gagccatcga
     8701 gtttacatgc gccacgcggc tcacctcccc agagactcct caccttcgat aaaggcccgc
     8761 agctcctcct cgtcgggcag tccctcgtag tctcctaagg tcgcgacgct cagggcgccc
     8821 atcgcgttag ccacgcgcag tcggtcttcc gcgggcaggt tccacagatg gccggccagg
     8881 tatccagcgg cgaaggagtc gccggctccc acggggtcga cctgcgccac cgtgaaggcc
     8941 tggtgctcta gggccttgcc gtcgatcagg gctaggcttc ccgcactgcc cctcttgagg
     9001 actacctcct ccggtccttc acgtgccagc tcacgcacca gggcctcgtc gtcgcgggcc
     9061 cagagcgccc tcgcctcctc gtccccggcg aagagcaggt agacgtcggg caggatctcc
     9121 tcaacgaact ctcgcgcctc ctccgtttcc cacagcttcg agcggtagtt gacgtcgaag
     9181 ctgacgcgtg cccccgacgc gcaggcctcc ctggctgccc acaggacgaa agcgcggcac
     9241 tcttccgaga gagcaggcgt gatgcccgtc aggtgcacga acttcgctcc tcccaggtag
     9301 tccgggtcga aggcgtctgt gtgcatggtc gaggcggccg atcccagacg gtagtagtag
     9361 acccggacgt ctgtgccgat ctgctcccga atatatagtc cggtcggacg ctcctttatc
     9421 cggcgcacct gtgatgtgtc caccccttcg gcgcggatgc ggtcgaggac gagttggccc
     9481 ggctcgtcgc cgccgaggta cccggcccat ccggcggaga agccgaggcg ggagagggcg
     9541 atggcgaggt tcgactcggc gcctcctatc ttgagctcca ggctagtggc gtggcgcaga
     9601 cgcccaggcc gcggcggaac gccgaggacc atcgtctcac ctgctgtgat cacgtcgagg
     9661 ttctctgcca cctgtggtct ctggttcgac tcaactttat cctggcatta tctctcctgg
     9721 agggcgattc tgcttgtgct aatctggtcg ccatcgatgc cggttcgagg agagtttcgg
     9781 cgatggagat tcgagaaggg gaggtggtcg ggatcgacct gatcggcagg atcgacgcgc
     9841 tggaggtagg agaacggtcg ctagccttct gggggctcgg gcaggtgggg gtagcgatca
     9901 a
//
LOCUS       DASTKM010000273         8604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1508119, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000273 DASTKM010000000
VERSION     DASTKM010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508119"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(564..1055)
                     /locus_tag="VFQ10_11035"
     CDS             complement(564..1055)
                     /locus_tag="VFQ10_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9965918.1"
                     /translation="MNSRSSRDQDAERRRRRSTRAIKESLRELSIQLSLLNHHVGAHL
                     DLKDVDLDCLDLIARHGPLSPSALARRGGLHPATMTGIIDRLERGGWVARERDPSDRR
                     AVVVKALRERNAELMRLYSGMNASMDEICAGYGDTELELLADFLRRTANAGGRATDKL
                     AGD"
     gene            1137..1775
                     /locus_tag="VFQ10_11040"
     CDS             1137..1775
                     /locus_tag="VFQ10_11040"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HET9965919.1"
                     /translation="MTRMNVGRVLSRCELVTIHGDPVQIPEPQRLMHLQFRRYAGCPV
                     CNLHLRSIARRHEEILAAGIREVVVFHSSADAMLGFQGGLPFAAIADPEKKLYSEFGV
                     EKMSPLAALIAALTPRSWQAAGRALRRAPSLRGAAGKGEEHFGLPADFLIGPRGRVLA
                     AKYGEYVDDQWSVDELLDLAKNARHIVGASPAPTGATTPTTRRSVARAPASR"
     gene            1905..2357
                     /locus_tag="VFQ10_11045"
     CDS             1905..2357
                     /locus_tag="VFQ10_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9965920.1"
                     /translation="MDLGLAGRHFTVAVFVVLEGKVLLHFHRKLGMWLPPGGHIEEGE
                     LPDEAAVREVWEETGVQVELVGERREDIVNPLQLHRPAGVQLENIEPGHQHIDLIYFA
                     RPAGSTEIRDEFAEEKVGWYAPEDWGGMPLNAEVRGWCERAFAALEDA"
     gene            complement(2513..3967)
                     /locus_tag="VFQ10_11050"
     CDS             complement(2513..3967)
                     /locus_tag="VFQ10_11050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9965921.1"
                     /translation="MSAAKAKDGGSRRWWALILLCVAQFVDVLDINAVIVALPTIGRE
                     LGFSHGDLQWVVTSYVLFFGGFLLFAGRLADLFGRRKMFMVGLALFAASSLLCGLAWS
                     PLMLVVFRAAQGLGAAIVAPAALSIISTTFPEGRERNFAMGLWTAVAAGGGAAGLVLG
                     GLITDVLGWEWIFFVNVPLGAAGVALSFVLLEADRGERTSRQLDLPGAVTVTAGLVLL
                     VYGLTRAEESGFGSPLTLATIVLALGLLAVFLFVEHSVADPLMPLRVFRERDLSGSAL
                     VAFANTATTSPVGIIAAIYLQEVLAYSPTLAGLLGLPFSLSVVAGSFLGSRLTGTLGA
                     RHTMALGLVGICGATLLITGISAEGGLPYVVANAALSGLALGCSAVASTTRGTSAVRE
                     EERGLASGLLNSSAQVGTALGLAVFFTVAVARTEAVALGGEPSAEALVVGYRWAFFVG
                     AGIAALGAVAALRLVGRDARHEVDSSRELSGGKD"
     gene            4102..5169
                     /locus_tag="VFQ10_11055"
     CDS             4102..5169
                     /locus_tag="VFQ10_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9965922.1"
                     /translation="MARVAEELGVSSMTISNAYNRPERVSESLREQIFETAERLGYAG
                     PDPVARSLRRQKTNLAGVLYSNPLSYAFDDAAQVLFLKGVATATEEAGMGLVLVPGSV
                     GSSPNERASAVMDAAVDGFVVYSIADDDPLVEAALRRRLPMVIADQPLLEDVPFVGID
                     NESAAREVARHLVDLGHEHFGVVSFSIAAADRPPGIADSRRQRSATLRVTRARLAGYE
                     AVLEAAGLPWKGVPVYECFGSSKALGREVADALLSQDPSLTAILALSDQLALGVIEAA
                     KEHGLSVPEDLSVAGFDDVPEAATSRPPLTTVHQDHTKKGELAGRLLVAQLREEETED
                     TDLLPARLVVRGSTGRPPGAS"
     gene            complement(5581..7047)
                     /locus_tag="VFQ10_11060"
     CDS             complement(5581..7047)
                     /locus_tag="VFQ10_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9965923.1"
                     /translation="MRDLTVALYEHLQRQSLGYYGERQTGELLTRLSKDTADMEPFMA
                     HDIPDIIVNVSMLVGISAILFSLDPELAALTLIPLPFLAYFVVRFSDRMHEAFTRARE
                     HYGALSALLQDNLSGMKEIQVFSGEAREKRRVRGQAQRHTRDRLKANKLEALWTPGIE
                     VIAGAGTVIVAWFGGRAALQGKLPVEDLVAFVLYLGLFYQPLRLLARTSESFQEARTG
                     AQHVWEVLDVQPDVVDPPGGVDSGRVRGKVHFESVDFEYEPDLPVLNNVSFEVQPGQT
                     LALVGPTGAGKSTIVSLIPRFYDPKRGRVLIDGIDVSEMRLASVRRNVSMVLQDTFLF
                     AGTVKENLRFGNEGASDEEIVVATKAANAHSFIEALPQGYDTRLGERGVRLSGGQKQR
                     LSIARAILKDAPILILDEATSSVDTQTEEEIQKALHELMQERTAIVIAHRLSTVRHAD
                     LIAVLEGGSIVELGTHEHLMRCKGLYRNLYDRQFGAAA"
     gene            complement(7054..7239)
                     /locus_tag="VFQ10_11065"
     CDS             complement(7054..7239)
                     /locus_tag="VFQ10_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965924.1"
                     /translation="MMLAGPWLIRDLVRIIAAESQGGGWKEAVRPVSWLTLGLVASFL
                     LRSAFLFLMGYIAHIVA"
     gene            7517..7987
                     /locus_tag="VFQ10_11070"
     CDS             7517..7987
                     /locus_tag="VFQ10_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965925.1"
                     /translation="MLGLILMSTQAFLYNAVLFTYALVLSSFYGVSAANAPYYLAAFA
                     IGNLAGPLLLGRYFDRVDRVPMISGCYIASGAILGLTSYLFSQNLLGATTLTMLWTVM
                     FFFASAAASSAYLTVSEVFPMEIRAMAIALFYSIATALGGDHGPHHLRPTHRHG"
BASE COUNT         1670 a         2633 c         2719 g         1582 t
ORIGIN      
        1 tgatgcgcta gctgagcaca cgaaaagagt agcgccgctg ccacactaca ggccacctgc
       61 gaccctgctg gcgcctcccc gcggaccgag aagagggcgt gttccgggaa ggtcgctggt
      121 cgcaggttct cgtgagtcac gcacgcgttt gccacaccat gtctagcggg tacagggtct
      181 ttcgccttga tccaatgaag agaagaggag gaggttcgcc ttgagcgagc aacacaccga
      241 gcaatcgata aaggtccgcc agatcaccga cgtccacgcc aattggagca gttagggacc
      301 tctggagaac ggcaagttct cctaccagct gatactggac aacggggccg aggaggctct
      361 catcatgccc actgcagacg atgcgaaggt cttgcgcgat cttatccacg acgcagacac
      421 cctctattgg gacactgaga gggagatgat tatcttcgga aaggtaaact gagctgccgc
      481 tgccccggaa cgaatagaag cgtttgggcg gtgcttatcg tttgcgcggt cgacccagct
      541 cttactctcg ctgcggcgga agctcagtcg cctgccagct tgtcggtcgc gcgtcctccg
      601 gcgtttgcgg tgcggcggag aaagtcggcg agcagttcga gttcggtgtc cccatatccg
      661 gcacagatct catccatcga ggcgttcatc cccgagtaga ggcgcatcag ctccgcgttc
      721 cgctcgcgta gggccttaac cacgacagcg cggcggtcgg acgggtcgcg ctcgcgtgcc
      781 acccagccac cgcgctctag tctgtcgatg atgccggtca tggtggcggg gtgcagcccg
      841 ccacgtcgtg cgagggcgct cgggctgagc ggaccgtggc gagcgatgag gtcgaggcaa
      901 tcgaggtcga catccttgag atcaaggtgt gcgccgacgt gatggttgag cagggacagt
      961 tggatgctca gctcgcgcag tgactccttg atggcgcggg tcgaccgtcg tcggcgtcgc
     1021 tctgcgtcct gatcccttga tgatcttgaa ttcatatgat attgtctcca tatattctga
     1081 tatacagaag atagtgtaca cgggagagag tcacccgtcc agacgaaagg atcgtcatga
     1141 ccaggatgaa cgtgggacga gtcctgagcc gctgtgaact agtgaccata cacggcgatc
     1201 cggtgcagat acccgaaccc cagcggctca tgcatctgca gttccgccgg tacgcgggct
     1261 gcccggtctg caatctgcac ctcaggtcca tcgcccggcg acatgaggag atcctcgcgg
     1321 ccgggatccg cgaggtcgtc gtgtttcact cgagcgccga tgcgatgctg gggttccagg
     1381 gtgggctgcc gttcgcggcg atcgcggacc ccgaaaagaa gctctactcc gagttcggcg
     1441 tggagaagat gtctcccctt gccgcactca tagccgcgct gaccccacgc tcgtggcagg
     1501 cggccggccg cgccttgagg cgggcgccca gcctgcgcgg agctgcaggc aagggtgagg
     1561 agcacttcgg cctcccggcg gacttcctga tcggcccccg cggtcgggtt ctcgcggcga
     1621 agtacggcga atatgtggat gaccagtggt cggtggacga actgctcgat ctagccaaga
     1681 atgcccggca tatagttgga gccagtccgg ctccaactgg tgccaccacc ccgactacgc
     1741 ggaggagcgt cgcgcgagcg ccggctagcc gctgatctgg tcagacagca tgttgtgatc
     1801 cactacggtg gctaggctag gaggtcaaac gtggcctaca ctccctgctg ctttctctgt
     1861 gatggtgcct accttacggg tactctagtg tacccttggc ccatatggac cttggactag
     1921 ctggcaggca cttcaccgtc gcggtcttcg tggtgttgga gggtaaggta ctcttgcact
     1981 tccaccgcaa gctcgggatg tggcttcctc cgggcgggca catcgaggag ggcgagctgc
     2041 cggatgaggc cgcggtgcgt gaggtgtggg aggagacggg ggtgcaagtt gagctggtgg
     2101 gggagaggcg cgaggatatc gtgaacccac tgcagctgca tcgaccagcc ggggtgcagc
     2161 ttgagaacat cgaacctggg catcagcaca tagacctgat ctacttcgca aggcccgcgg
     2221 gttccacaga gatccgcgat gagttcgccg aggagaaggt cggctggtac gcaccagaag
     2281 actggggtgg gatgcccttg aacgccgagg tcaggggttg gtgcgagaga gcgtttgcgg
     2341 ctctcgagga tgcatgatct tcgccattcc acagacctag cggatgctat gcgatgagtc
     2401 gcacggtcgt cgtcgcgccg tcggggccga ggcggacgca gaagctcacc cccggcgttg
     2461 ttccagcttg catgggccca cgagaaggcg gtggctattc gcccacggtg cctcagtcct
     2521 ttccgccgct gagctcgcgg gaagagtcga cctcgtgcct ggcgtcccgg cccacgagcc
     2581 ggagtgcagc caccgccccg agcgccgcta tgccggcgcc cacgaagaac gcccacctgt
     2641 atcccacgac gagcgcttcc gcgctaggct ctccgcccag ggccaccgcc tctgttcggg
     2701 cgacggccac cgtgaagaag accgccaacc ccaaggccgt gccgacctgc gcggaggagt
     2761 tcaggaggcc agaggcgagc ccgcgctctt cctccctcac cgccgaggtg ccgcgggtcg
     2821 tggaggcgac cgccgagcag cccagggcca aacccgagag agcggcgttc gcgaccacgt
     2881 aaggaaggcc gccctcggcg gatattccgg tgataagcaa cgtcgcgcca cagatgccca
     2941 ccagccccag cgccatagta tgtctcgccc ccaaagtccc cgtgagccgg gagcccagga
     3001 aggagccggc cacgaccgag aggctgaagg gcaaacctaa aagccccgcc agggtgggag
     3061 agtaggccag gacctcctgc aggtagatgg ccgcgatgat gcccaccggg ctggtcgtcg
     3121 ccgtgttcgc gaaggccacc agtgctgagc cagataggtc ccgctcccgg aagacgcgga
     3181 gcggcatcag cggatcggcc acactgtgct caacgaagag gaagaccgca agcaggccca
     3241 gggccaggac tatagttgcc agcgtgaggg gcgatccgaa gcccgactcc tccgccctgg
     3301 tgaggccata gactaagagc acgagccccg ccgtaaccgt caccgcgccc ggcagatcga
     3361 gctggcgaga cgttcgctcc ccgcggtcgg cttcgagcag cacgaaggag agcgcgacgc
     3421 ccgccgcgcc gaggggtacg ttgacgaaga agatccactc ccacccgagc acgtcggtga
     3481 tcagaccgcc gaggaccaac ccggccgcgc ccccgccggc cgccaccgcc gtccacaatc
     3541 ccattgcaaa attgcgctct ctgccctcag ggaacgtcgt cgagatgatg gagagggccg
     3601 ccggcgccac gatcgccgcc ccgaggccct gagcggcacg gaatacgacc agcatcagtg
     3661 gcgaccaggc aagcccgcat agcagcgaag aggcagcaaa cagggcgagc cctaccataa
     3721 acatcttgcg cctgccgaaa aggtcggcga gtctccccgc aaagagaaga aagccaccaa
     3781 agaagagcac gtaggaggtc accacccact gcaggtcccc gtgcgaaaac ccgagctctc
     3841 gccctatcgt gggcaacgcg acgatgacgg cgttgatgtc gagtacgtca acgaactgcg
     3901 ccacacacag caagatcagc gcccaccacc tgcgagagcc tccgtccttc gctttcgcgg
     3961 cgctcaatct ttcctccaaa cgtcccaaga tttcactttt aacgttacat tttaacgtca
     4021 aactaatgta tcataaagat gatggaacgc aagcggtcgc aagcggcttc cggcgggcgg
     4081 tcctccgggc ggagggcaac catggcgcgc gttgctgagg agctcggagt ctcgtccatg
     4141 acgatctcga acgcctacaa ccgccccgag cgggtctctg agtccttgcg ggagcagatc
     4201 ttcgagacgg ccgagcgcct gggctacgcg gggcccgatc cggtggcgcg ctccctgagg
     4261 agacaaaaga ctaatctggc aggcgtgctc tactcaaacc cgctctcgta cgccttcgac
     4321 gatgcggctc aagtgctgtt cctcaagggc gtcgctacag ccaccgagga ggcggggatg
     4381 gggctcgtgc tcgtgcccgg gtcggtgggc tcttcgccca acgagcgtgc ctcggcggtg
     4441 atggacgcgg ccgtcgatgg gttcgtcgtc tactcgatcg ccgacgacga cccgctggtc
     4501 gaggcggccc taaggaggcg gctgccgatg gtgatcgcgg accaacccct cctggaggat
     4561 gtgcccttcg tagggatcga caacgagtcg gccgcgaggg aggtggcgag gcacctggtg
     4621 gacctgggac acgagcactt cggcgtcgtt tcgttctcca tcgcggcggc agaccggccc
     4681 cccggaatcg ccgattcgcg tcgtcagcgg tctgcgacct tacgggtgac ccgcgcaaga
     4741 ctcgcgggct acgaagccgt cctggaagcc gccgggcttc cttggaaagg agtgccggtc
     4801 tacgaatgct tcggcagctc taaggcgttg ggccgcgagg tggctgacgc cttgctgtca
     4861 caggatccct ctctgacggc catactggcg ctgagcgacc agctcgcttt gggtgtcatc
     4921 gaggctgcca aagaacatgg tctgtcggtg cccgaagatc tctcggtagc gggtttcgat
     4981 gacgtgcccg aggccgcgac atcgaggccg ccccttacga cggtccacca agatcacacc
     5041 aagaagggcg agttggcagg acgcctactg gtcgcgcagc tccgcgagga agagacggag
     5101 gatacggacc ttttgcctgc tcgactcgta gtacgaggct cgaccggtcg gccaccggga
     5161 gcatcttagg cagcgtctaa gggctcttcg ggatcggagg tgaacgcgct cctaccgttc
     5221 cttgcgctcg ctgcaggctg agggagtccg gccgattgtc tgcgcggcat aggcacggtc
     5281 aagaacggac atcctcggcg accgccaaga acggcattcc gaagcgctca ttactccgta
     5341 cgactgtcga atcaataagg tgtgtttctg atacgacgtg ccagggatgc ccgtagaata
     5401 cgggagcgag aaaactgcag agagcgcctt ctgcaaggct acaggcggct gaagcgcaag
     5461 ccagacccgg tgaccacagc gatccctgtt atgaggctca tcgagaaacc accgacactt
     5521 gagcacgttc cctgctactc tggcaagcgt gtttcgccgt gccttactgc cggatctctt
     5581 ctaggcagcg gcgccgaact gccgatcgta gaggttgcga tagagtcctt tgcatcgcat
     5641 taggtgctcg tgagtgccta gctccacgat gctacctccc tctaagacag cgatcaggtc
     5701 agcgtggcgc acggtcgaga gccggtgggc gatcacgatc gcggtacgct cctgcataag
     5761 ttcgtgcaat gctttctgga tctcctcctc ggtctgggta tcgaccgaag aggtagcctc
     5821 gtcgagtatg aggattgggg catctttaag gattgctcgc gctatggaga gtcgctgctt
     5881 ctgcccgccc gagagcctga ctccgcgttc gcccaagcgg gtgtcgtacc cttgaggcaa
     5941 cgcctcgatg aagctgtggg cgttggctgc cttggtcgcc acgacgatct cctcgtctga
     6001 ggctccctcg ttgccgaagc gcaggttctc ctttacagtt ccggcgaaga ggaatgtatc
     6061 ctgcaagacc atgctgacgt tgcggcgcac ggacgccagg cgcatctcac taacgtctat
     6121 gccgtcgatc agaacccgtc cccgcttggg gtcgtagaac cgggggatca agctgacgat
     6181 cgtgctcttg cctgcgccag tgggtccgac taaggcgagt gtctgcccgg gctggacctc
     6241 aaaggagacg ttattgagga cgggcaggtc cggctcatac tcgaagtcca cgctctcgaa
     6301 gtgaacctta ccccggactc ggccagagtc cactcctccc ggcgggtcga ccacatctgg
     6361 ctgtacatca agcacctccc agacgtgctg ggctccggtc ctggcttctt ggaagctttc
     6421 gctcgtacgc gccagcagcc tgagcggttg gtagaagaga ccgaggtaga gcacgaaggc
     6481 cactaaatcc tcgaccggca gtttaccctg cagtgccgcc cggcccccga accacgccac
     6541 gatcacggtg ccggcaccgg cgataacttc gatgcccggg gtccacagag cctcgagctt
     6601 gttggcttta agcctatctc gcgtgtgtcg ctgcgcctgg ccccgcacgc gcctcttctc
     6661 acgcgcctcc cctgagaaga cctgaatctc cttcatccca gataggttat cctgaagcag
     6721 agcgctcagg gcaccatagt gctcccgggc acgcgtgaac gcttcgtgca tccggtcgct
     6781 gaacctcacg acgaagtagg ccaggaaggg cagcgggatc agcgtcagcg ccgctagctc
     6841 agggtcgagg ctgaatagga tcgccgagat gcctacgagc attgagacgt tcacgatgat
     6901 gtcggggatg tcgtgggcca tgaacggttc catgtccgcg gtatccttgc tcaggcgggt
     6961 tagcagctct ccggtttggc gctcgccgta gtagccaagc gactggcgct ggaggtgctc
     7021 gtagagggcg acggtgaggt cgcgcacgta gcttcaggcc acgatgtggg cgatgtagcc
     7081 catcaagaac agaaaggcgg accgcagcaa aaaggatgct accagaccga gcgtgagcca
     7141 agagaccggt ctcaccgcct ccttccagcc gccaccctgg ctctcggcag cgatgattcg
     7201 gaccaggtcc cggatcagcc atggcccagc cagcatcatc gccgtggctc ctaaagcgca
     7261 taacactaca agtagcgcgt gccagcggta gggtctcaag taagggagca atttgctcaa
     7321 cgagcgcaca acggcaatct tttcccgtta ggtgacaggc taaactcgat tactggggcg
     7381 aaagtcttta gcattctggg acctcagtgc tctatgtatg ggatcctgtt agagggaggt
     7441 gggaggcggc taggctctga cgagtacttt tcagcgatgc tcgtagaagg tgttttctac
     7501 gtacccgagg cgcaccgtgc tgggactcat actcatgtcc acccaggcat tcctctacaa
     7561 tgccgtcctt ttcacctacg ccctggttct gagtagcttc tacggagtat ccgcggccaa
     7621 tgccccctac tacctggcgg ccttcgccat cggcaacctg gcgggaccgc tcctgctcgg
     7681 gcgctacttt gatcgggtgg accgggtgcc gatgataagc ggctgctaca tagcgtccgg
     7741 ggccatcctt gggctgacga gctacctgtt ctcccagaat ctgctgggcg ccaccaccct
     7801 gacgatgctc tggacggtca tgttcttctt cgcctcggcg gctgccagct cggcttacct
     7861 gacggtgagt gaggtcttcc caatggagat aagggctatg gccatagcgc tgttttactc
     7921 catagccacg gctttggggg gggatcacgg gccccatcat cttcggccaa ctcatcggca
     7981 cgggtaacag atctagcctg ctgatcggat tcggcatagc tgcagtactg atgatcgggg
     8041 tggccatcgc ggagctgatc ctcggcgtca gggcagagcg gcgctcccta gagagcattg
     8101 ccgcgccgct aaccgccatt cagagagaaa ccggggccac tgcctgaggg aagtacacgg
     8161 cgcaaaggac tacgcaggag cgatgtcttc tttgtctttc ggctctttgt ctttaggcta
     8221 agagcgcggc gaggcatgga gatattagcc tgcggtacgc gaggatctag acagggggcg
     8281 gctggtcggc tgccttattc acccgagtgc gtggaaggtg aattctcaga agttgaactt
     8341 ccgctttatg gagttctaag aagttcgcca aggattcatc acggattcat cgccgcttgc
     8401 tagcgtggta gcgttgtaga ccacgacggg cgtgacgaga gcagcgatcg agaggaggag
     8461 gccatgacag aagaaaacca atgcggccct gaaacagatt ccgacttctt ctatgagctg
     8521 agggcagtgt tcgacaagta cccagatgcg gcacagacgt attccatcca ctgcaggacc
     8581 cttgaaagcg acatcatgcg tatt
//
LOCUS       DASTKM010000274         7259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1509029, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000274 DASTKM010000000
VERSION     DASTKM010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509029"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..315)
                     /locus_tag="VFQ10_11075"
     CDS             complement(10..315)
                     /locus_tag="VFQ10_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965926.1"
                     /translation="MKPRVTAQLHSSSGRSSKILRDHTHIETVVEVAGVGRLVLDVDS
                     GGGYSLRAYPEGSEDPSRDLLAVGVVHADGIVAIHPERLAAFNGEQGAPGPERRPNV"
     regulatory      complement(396..481)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            758..1183
                     /locus_tag="VFQ10_11080"
     CDS             758..1183
                     /locus_tag="VFQ10_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965927.1"
                     /translation="MLRRMVLLGGALLVGVIVLSGVALAQDIRGTFKQDELTGTNQLD
                     RIYGLGAADTIVGLGGNDDCYGGSGDDEVSCRAGNDRIDGGFGTDDLFGGPGNDTISA
                     ADGFEDNVNCGTGDNDTAYVDNQDIVKQNCENIWEAQAQ"
     gene            complement(1501..1734)
                     /locus_tag="VFQ10_11085"
     CDS             complement(1501..1734)
                     /locus_tag="VFQ10_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965928.1"
                     /translation="MEDQGVPEAWIGQEVILHTVSDREFLANLVEVKDFGFAYQFRDS
                     EDIIFAPWSVLRWMRQPGDDAEHMRRGELGEFD"
     gene            complement(2230..3972)
                     /locus_tag="VFQ10_11090"
     CDS             complement(2230..3972)
                     /locus_tag="VFQ10_11090"
                     /inference="COORDINATES: protein motif:HMM:NF011539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HET9965929.1"
                     /translation="MEERTQDYRLVTTAEDLAAVAKTLQSAEAIGVDIETTALSPHDG
                     GMRLLQLATPEETFVIDVFEVGDLSPLTEVLEGGPVKVGHNLKFDYQFLHAMYGISLS
                     PLFDTMLAAQVLDGGNYAPSYSLEAVVERYLDESLDKSQQRSDWSGELSRGQLEYAAR
                     DAAILLPLRERLAEALEAEELGFVSKIEFAAVASIAEMELAGIKLDLQRWKELERVVR
                     ERRDRAALELESHFPEPEGVLPLEGLGPRLNLNSPQQITDAFRSLGIELPDTKVWTLL
                     KVEHPAAQALLRYRELQKKLGTYLETYPKFIHKKTGRIHANFLQCRVPTGRLACAAPN
                     IQQIPHEDEFRRCFVAEDGNTLVIADYSQIELRILAEVSEDPAFVGAFQRGVDLHRLT
                     AATMYNVSMDEVTKAQRTAAKRINFGLMYGRGARSLSAQLGTDEERGRQLIDEYFANY
                     PKVQRFLQRTANRATRDRTLRTLAGRVRKFGNDPVADDRGAMRREAMNYPIQGASADI
                     AKLALSYIRRELKDTDARLINSIHDEFVVECAEELAPEVSEKTRQAMTRAGEKLLEKV
                     PVEVEVEVSREWRK"
     gene            4041..5150
                     /locus_tag="VFQ10_11095"
     CDS             4041..5150
                     /locus_tag="VFQ10_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HET9965930.1"
                     /translation="MMGGSFKIGRAFGIDVKVHWSFFLLLAFFGYLAFRDSGSLVDAL
                     VTVGIVVALFFCVLLHEFGHSLVAIRLGSEVQDITLLPIGGLARMKSLPERPIDEVKV
                     AVAGPLVNVVLAPVFFGLALLFGGNLSVPTDIISGVGSLGQVFATLGIINVALVVFNL
                     IPAFPMDGGRVLRGLLATRLGLLRATDISSTVGQAFAVLFILFGFFTSNFLLAIIGFF
                     VFLGASGEGQLVRQREQLRGLTVSDVMGTKGRTETVTPYHNFGQILDSVIHGYQEDFP
                     VLDEEGHLVGIITRNEIMAAAHSPDRYAIVRDLMKSEFPTISPDADLFTDGNRILQES
                     GLRALPVVKDGNLVGMLTVDDVGQAALLRDPRKRR"
     gene            5218..5820
                     /locus_tag="VFQ10_11100"
     CDS             5218..5820
                     /locus_tag="VFQ10_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9965931.1"
                     /translation="MKTVVEEIEAHIARVGTHPVYGYAHCLRVFALAEELAASEGVSY
                     DVEILRAAALLHDVGLYKAYALREPPDHAKRSAMVASRILQDWDFPPQASQAVIEAIE
                     HHPPGAPTSTASETILLKDAVGLDYLGAIGISRMLAMVGTEDDVPDIPTAIWNAESLR
                     QKIPDLLVLQSSKDLAARRIREMEDFLEDLRDSTAKLKLL"
     gene            5845..5985
                     /locus_tag="VFQ10_11105"
     CDS             5845..5985
                     /locus_tag="VFQ10_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965932.1"
                     /translation="MQAARRDRAAYEGPWNFWNGVRTVFSSMAFLALIGACLLREDRS
                     GR"
     gene            complement(6033..6236)
                     /locus_tag="VFQ10_11110"
     CDS             complement(6033..6236)
                     /locus_tag="VFQ10_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965933.1"
                     /translation="MTAFGLLLILTGLAGLSFGAYALLRGGRGQQGRIGPIPERGVHL
                     IAGTRMLLVGALCVAAGLYLLLS"
     gene            complement(6247..>7259)
                     /locus_tag="VFQ10_11115"
     CDS             complement(6247..>7259)
                     /locus_tag="VFQ10_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9965934.1"
                     /translation="YYGFMGEPALAKATFERWTNRVPEDADMLINAGLTLFDAADYVE
                     ALEFFEKAIEAAAEEVQKCGARTFRANALDMLGRYAEAVGAYEEVIAGASDWWEAHAN
                     LGICHARNGNLEKAEAAFRRGLEDCPGSPEIRDELAAHLLALRKDLPEALRLSEEAVA
                     LGRDEIRHLITLGEARLALGDEAGAAEAYGAVLALDPENPEAHLELGIINERKGEMER
                     AEEHFVESLKVDPANPRTLYSYASLYYTADDLEAAEEILERAIAADARYSPALSALAS
                     IQARRGEYGDALDYIEKAVDAGESDVEHFRSALEFAPLHADPRFRTLLSRMVGDRSNG
                     HG"
BASE COUNT         1298 a         2261 c         2224 g         1476 t
ORIGIN      
        1 ataggaacct cacacattgg gccttcgctc ggggcctggg gcaccttgct caccattaaa
       61 agcagccaac cgctccgggt ggatcgctac gatcccgtcg gcatgcacca cgccaactgc
      121 gagcaggtct cggctcggat cttcgctgcc ctcgggatac gcccgtagcg agtagccgcc
      181 tccgctgtcc acatctagta ccaacctacc caccccggcc acttccacga cggtctcgat
      241 gtgcgtgtgg tctctgagga tcttcgagct cctgcccgaa gatgaatgaa gctgagctgt
      301 caccctaggt ttcatgcgtg tccgtcccgg gtcatacgac agctcaaggc cctctcacct
      361 cctttcgccc aaagcgaaac gcaaccgacc accgcattct cggtagtcgg ttgcgctact
      421 ggctctgccc gtctcttaag cgcccataat tggggcgggc ttctcagcct ctactttggg
      481 ttgttccttt acggttgcac atagtagcaa ctgagggagg cgtgtcaaca cgttttgctt
      541 gcgtcgacct tacgagaact aaccttccac gcactcgggt gaataagcct atctgcaggc
      601 atttggcacc ccactactaa caactagatg caccaatagt ccctctttga ggggtgcgtg
      661 gtaccttcct gatggtgtag agttctctcg ttagctaacc ggggcgggca taaaaggggt
      721 tctggccgcc ccactattgg taaaggaggt aatgctcatg ctacgccgga tggttctact
      781 tggcggggcg ttgttggtag gagtgatcgt gcttagcggg gtggcgctgg cccaagacat
      841 aaggggcact tttaagcagg atgagctcac aggcaccaat cagctggacc ggatctatgg
      901 actcggtgcc gcggacacga tcgtgggtct gggaggcaac gacgattgct acggcggcag
      961 tggtgacgac gaggtaagtt gcagggccgg taacgaccgc atagacggcg ggttcggtac
     1021 cgacgacttg tttggtggtc ccggcaatga cacgatcagc gctgcggacg gtttcgagga
     1081 caacgtcaac tgcggcactg gtgacaacga cacggcctac gtcgataacc aggacatcgt
     1141 caaacagaac tgcgaaaaca tctgggaggc tcaagcgcaa taagccaata actactggca
     1201 ccgcccagga ggtagtagag gagagaaacg gaggcactga gatcagggta gctccctgga
     1261 gcctccgttc tcctcttcca atcgcaccgc ccgaagccat cggctgatag cttactttcc
     1321 cacgttgacg atacgttgtc gccctgtttc agcgtgaacg gattacgggt gcccaataga
     1381 aacatccacg aatggttcgc ctgtgccgcg ggtgaaaggg cacagagagg gggaatagaa
     1441 gcgcagtacc ggggccgaaa ggccctgaac gtcatcctcg cggctcttag aaacaacaga
     1501 ttagtcgaac tccccaagtt ctccgcgtcg catgtgctcg gcatcatcac ctggttggcg
     1561 catccaacgg agcacgctcc aaggtgcaaa tattatgtcc tcactgtctc taaactgata
     1621 agcaaagcca aaatccttga cctctacgag atttgctaga aactctcggt cggaaacggt
     1681 gtggaggatt acctcttgcc cgatccacgc ctccggcact ccctggtcct ccataccgtc
     1741 ctccgtactg gtatagaacc gtctgtgcac cctagcacgg tttgcgctgg ccggcagaag
     1801 tcatgctcgc tcgtctcgag ggcattcatc ggtgattatc atccgacgct accgtagagg
     1861 atgaccggct ctttctccgc gggtgttgta caggccgacg ccgtttcagg caccgcgagc
     1921 tcctcgaggt ccagggggat cacaaatcga aggcgcaggg ttcgccgcct gagacggacg
     1981 cgtagactct gctgttccta acgaactccg gggtgatagt caggctatct ctccagtgct
     2041 ggatccagga ctccggatac ccccagtaca gtttcgccgc taaggcgata cgtgtaaggg
     2101 cgtcggcatc ctcaggtgtc gcgggcagta tatccaccgt gctattcaaa acgaaggtga
     2161 accgccgata ggcctaagtg ctaccccgag cgtgttccga gaagccgcct gctctggcgg
     2221 gcggagtcat cacttcctcc actcgcgcga gacttcgact tcgacctcga caggtacctt
     2281 ctcgagcagc ttctcacccg ccctggtcat ggcttgcctg gtcttctccg aaacctcagg
     2341 agcaagctcc tcggcgcatt cgacgacgaa ctcgtcgtgg atggagttta tgagacgggc
     2401 gtcagtgtcc ttaagttccc tgcggatgta ggatagggcg agcttggcta tgtctgcgct
     2461 ggcaccctgg atcggatagt tcatcgcctc gcggcgcatc gcgccccggt cgtctgccac
     2521 aggatcgttg ccgaacttcc tgaccctgcc cgcgagcgtc ctgagggtcc tgtctctcgt
     2581 tgcccggttg gccgtacgct gcaaaaatcg ctgcaccttg gggtagttgg cgaagtactc
     2641 atctatgagc tgacgaccac gttcctcgtc cgtgccgagc tgtgccgaga ggctccgtgc
     2701 gccgcggccg tacatcaggc cgaagtttat gcgcttggcc gccgtgcgct gggccttggt
     2761 gacctcgtcc atggacacgt tgtacatcgt ggccgccgta agtcggtgga ggtccacgcc
     2821 cctctggaaa gccccgacga aagcgggatc ttctgagacc tcggcgagta tcctcagctc
     2881 tatctgcgag tagtccgcta tgacgagcgt gttgccatcc tcggccacga agcagcggcg
     2941 gaactcgtcc tcgtgcggga tctgctggat attcggagcc gcgcaggcga gacgcccggt
     3001 cggcacgcgg cactgcagga agttcgcgtg gatgcgtccc gtcttcttgt ggatgaactt
     3061 ggggtaggtc tcgaggtagg tgcctagctt tttctggagc tcgcggtagc ggagaagggc
     3121 ctgggcggca gggtgctcga ccttgaggag cgtccagacc ttggtatccg gaagctctat
     3181 cccgagtgac ctgaaggcgt ccgtgatctg ctgagggctg ttgaggttga ggcgtggacc
     3241 gagcccctcc agcggcagca cgccctcggg ttccgggaaa tgcgactcca gctccagggc
     3301 cgccctgtcc cttcgctcgc ggacgactct ctccagttcc ttccacctct gtaggtcgag
     3361 cttgatgcct gcgagttcca tctcggctat cgaggcgaca gcggcgaact ctatcttcga
     3421 gacgaatccc agctcctccg cctccagtgc ctccgcgaga cgttcgcgca aagggagcag
     3481 gatggccgcg tcgcgggcgg cgtactccag ctggccgcgg cttaactccc cagaccagtc
     3541 gctcctctgc tgggacttgt cgagcgactc gtcgaggtaa cgctcaacca cggcttccag
     3601 ggagtaggac ggcgcgtagt tgccgccatc caggacctgg gcggcgagca tcgtgtcgaa
     3661 gaggggtgag agcgagatcc cgtacatggc gtgcaagaat tggtagtcga acttcaggtt
     3721 gtgccccacc ttgaccggtc cgccttcgag cacctctgtg agtggcgaga ggtcaccgac
     3781 ctcgaagacg tctatgacga acgtctcctc tggggtggcg agctggagaa ggcgcatccc
     3841 tccgtcgtgg ggggagaggg ctgtggtctc gatgtccacg cctatggctt cggcgctttg
     3901 cagagtcttc gcgacggcgg caaggtcttc cgcggtcgta acgagacggt agtcctgggt
     3961 gcgttcctcc acccctcgat tctagcaggg agagggggtt tcggatcaca tcgccggccg
     4021 gcgggtgggg taaactcacc atgatgggtg gatctttcaa gattggacgc gctttcggca
     4081 tagacgtgaa ggtccactgg agcttcttcc tgctgctggc cttcttcggc tatctcgctt
     4141 tcagggattc cggcagcctt gtggacgcgc tcgtcaccgt cggtatagtg gtcgcgctgt
     4201 tcttctgcgt cctgcttcac gagttcggcc actcgctggt agcgatacgt ctcgggagcg
     4261 aggttcaaga catcacgctt ttgcccatcg gcgggctcgc caggatgaag tctcttccag
     4321 agagacccat cgacgaggtg aaggtcgccg tagccgggcc gctcgtcaac gtcgtgctag
     4381 cccctgtatt cttcgggctt gccctgctct tcggaggcaa cctctcggtc ccgacggaca
     4441 taatctcggg ggtagggtcc ctgggccagg tcttcgccac gctgggcatc atcaacgtcg
     4501 ccctcgtggt cttcaacttg ataccggcct ttccgatgga tggcggccgt gtgctacgcg
     4561 gcctgctcgc cacacgcctg ggactcttgc gcgccacgga catttcctcg accgtagggc
     4621 aggccttcgc cgtcctcttc atcctcttcg gcttctttac cagtaacttc ctgctcgcca
     4681 tcatcggctt cttcgtcttc cttggcgcga gcggcgaggg ccaactggtc cgccagcggg
     4741 agcagctgcg cgggctcacg gtctcagacg taatgggtac caagggccga accgagacag
     4801 taacgcccta ccacaacttc ggccagatcc tcgactccgt aatccacggt taccaggaag
     4861 acttccctgt cctcgacgag gaaggacatc tggtcggcat tatcacccgc aatgagatca
     4921 tggccgcggc ccactccccc gaccgctacg ccatcgtccg cgacctgatg aagtcagaat
     4981 ttccgaccat ctcgccagac gccgacctct ttacagacgg caaccgtatc ctgcaggaga
     5041 gcggcctcag ggccctgccc gttgtaaagg atggcaatct cgtggggatg ctaaccgtag
     5101 acgatgtggg ccaggccgcc ctgcttcgcg accctcgcaa acggcggtaa ggcgccctcc
     5161 cggctcctgg cgaagccacg ctcgcaccgg cgggacctat tcgctagact ctctgccatg
     5221 aaaacggtgg tagaagagat cgaggctcat atcgccaggg tcgggacgca tcctgtttac
     5281 gggtacgccc actgcctgag ggtcttcgct ctggccgaag agctggcggc ctcggaggga
     5341 gtctcctacg acgtcgaaat tctgcgcgcg gccgccctgt tacacgatgt cgggctctac
     5401 aaagcctacg ctttgaggga gccgcctgac cacgccaagc ggtcggcgat ggtggccagc
     5461 cgtatcctcc aggactggga tttccccccg caggcgagcc aggcggtcat agaggcgatc
     5521 gaacaccatc cgccaggagc gcccacgagc acggcttctg agaccattct cctcaaagac
     5581 gcggtcggcc tcgactacct tggcgccata ggcatctccc gcatgctggc catggtaggc
     5641 acggaagacg acgtgcctga tataccgacg gcgatctgga acgcggagag cctgcggcag
     5701 aagatccccg acctcctcgt cctgcagagc agcaaggatc tggccgcccg gcgtatccgc
     5761 gagatggaag attttctcga agacctgagg gactccacgg cgaagctgaa gcttctctag
     5821 catttaagct agtcggcatt ctgggtgcaa gctgctcggc gcgatcgggc cgcctacgag
     5881 gggccttgga acttctggaa cggcgtgcgg acggtgttct cgtcgatggc gttcctggcc
     5941 ctcatcggcg cctgcctctt gcgcgaggac cgcagcgggc ggtgaggggg cgaataccag
     6001 aggtgtccca cgcgtgcctg gtggcaagcc ggttagctaa ggagcaggta gagaccggcg
     6061 gcgacgcaga gggctccgac caggagcatc ctcgtcccgg cgatgaggtg gacgccgcgt
     6121 tctggtatag ggccgatcct accctgttgg ccccttccac cgcgcagcag ggcgtaggcg
     6181 ccaaaggaga ggccggccag ccccgtgagg atcaacagta gcccgaaagc cgtcaaggcc
     6241 ggcatcctaa ccgtgtccgt tgctcctgtc gccaaccatg cgggagagga gggtgcggaa
     6301 gcgtgggtcg gcgtgcaggg gcgcgaactc cagcgcgctc ctgaagtgct cgacgtcgct
     6361 ctcaccggca tccacggcct tctcgatgta gtcgagcgcg tcgccgtatt cgccgcggcg
     6421 ggcctggatg ctggcgagcg cagagagggc gggggagtat ctcgcgtcag ctgctatggc
     6481 ccgctccagg atctcctccg ctgcctccag gtcgtccgct gtgtagtaga ggctggcgta
     6541 cgagtagagg gtgcgggggt tcgcgggatc tactttgagt gactccacaa agtgctcttc
     6601 tgcccgctcc atctctccct tgcgctcgtt gataatgccg agctcgaggt gggcctctgg
     6661 gttctcaggg tccagagcaa gtacagcgcc gtaggcttcg gcggccccgg cttcgtctcc
     6721 cagggcgagc ctggcctccc ccagggttat gaggtgccgg atctcatccc gccccagcgc
     6781 cacggcctcc tctgagagcc tcaaggcctc gggcagatcc ttcctcaggg cgagcagatg
     6841 ggcggccagt tcgtccctga tctccggcga gccaggacag tcttcgaggc cccgcctgaa
     6901 ggccgcctcg gccttctcca ggttgccgtt gcgggcgtgg cagatcccga ggttcgcgtg
     6961 ggcctcccac cagtctgacg cccctgctat gacctcctcg taggccccca cggcttcggc
     7021 gtagcggccg agcatatcca gggcgttggc cctgaaggtc cgcgcgccgc acttctgcac
     7081 ctcctctgct gcggcctcga tggccttctc gaagaactcc agggcctcga catagtcggc
     7141 ggcgtcgaag agagtcaggc ccgcgtttat gagcatgtcg gcgtcttcgg gcacgcggtt
     7201 cgtccagcgc tcgaaggtgg cctttgccag cgcgggctcc cccataaagc cgtagtagc
//
LOCUS       DASTKM010000275         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1510522, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000275 DASTKM010000000
VERSION     DASTKM010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510522"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..440)
                     /locus_tag="VFQ10_11120"
     CDS             complement(183..440)
                     /locus_tag="VFQ10_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9965935.1"
                     /translation="MESRVSILTLGVSDLQRAVAFYRDGLGWPISEVGGEEVAFFKTG
                     GVVISLFPRVSFAADAGLYLDDVEHGGFSRVSLAHNVAEEG"
     gene            733..1368
                     /locus_tag="VFQ10_11125"
     CDS             733..1368
                     /locus_tag="VFQ10_11125"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET9965936.1"
                     /translation="MLVCTPLHFMTTMLALVLAYNPQLIYSTGFQLSVAAVFGILLLT
                     RPLKSLLERSLLRLLKSPPAHLSNLISVSLAAQIATSPIVAATFEQVSIVGVFTNLIA
                     VPLSGPILILGLLGSVAGNIYPLIAYPLNACNGFLVTMLIQTAQGSSSLPFASVTTPG
                     VTLFLVGLFYAGCVPPIVAGRVFSSERKFLWAALLLIWSALWLVLVAAGSI"
     gene            1538..2938
                     /locus_tag="VFQ10_11130"
     CDS             1538..2938
                     /locus_tag="VFQ10_11130"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9965937.1"
                     /translation="MRSKLAMGGLTLGAAWGAYSRLRRPIPLKGSYARAPTKILIIGG
                     GFGGLSVARGLARALRGTEDVGVALVDRMNYTTFWPMVPSVIPSNAEVRHVAHSLRRI
                     LKPLGVEFFQDEVVGVDFEARRVKTAERDYPYDYLVLAPGSRTTYFGTPGAEENAMDV
                     KGLRDALRVRNHVIDCFEEAEHLDGQAPDDLLTFVVVGGGPTGVEAAADMHDLMFDVL
                     EDDYPNVKFDRVRVVLVNAGDQILKGLDSSLVNAARRRLAAQRIEVINDAKVEEVRPD
                     AVVISGGRTIPTRTTVWAAGIEPPPLVGNLDVQKDHRGRILIDQYLQVKGRPGVYAVG
                     DCTSIQYDGPPVPALAQAAEQEGKRAAANLVAENKNQAPVAFRYRSVGQLVDLGEGSA
                     LVDILGVKLGGLLGAFIWKGVYLYELGYDLNRAHVLADWTFDLFSRPDTSKLFEESQR
                     AKYKQDNIAQQTSPET"
     gene            3123..>3710
                     /locus_tag="VFQ10_11135"
     CDS             3123..>3710
                     /locus_tag="VFQ10_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965938.1"
                     /translation="MTPLLYRMGHFCARHLVRVLALWLVVALTIVGVARIVGQETNDD
                     LSLPGTDSQAASDLLRDKFPEQANGSVPIAFRAPAGAKLSDTRYKKPIEQVTSAFGKD
                     SAVTHAVGPFSEQGADQLNKKRTIGYISLNLKDSPSELSIEEAQRIISLDRPLEKAGL
                     NPAAGGYLGQKVSKPSTHLSEVIGLTAAVLILLFTF"
BASE COUNT          775 a         1168 c         1074 g          693 t
ORIGIN      
        1 cacgggccta tgcaagctga ccagcaccca caaactggcg gatccggttt ggtcgccaga
       61 cggtggaaag atagccttcg ccgacaaggg agagatcaac gtgatcaaca ccgatggtag
      121 cgggcaggag gagcttgccg caggcacttc tgcgacgtga cttctgcgag tatcgcatcc
      181 acctacccct cctcagccac attgtgcgct agcgagacgc gcgagaaccc gccgtgctcc
      241 acatcatcta aatagaggcc tgcgtcggct gcgaacgaca ccctcgggaa gagggagatc
      301 accactccac ccgtcttgaa gaacgcgacc tcctcgccac caacctcgga gatgggccat
      361 cccaatccgt cgcgatagaa ggcgacggct cgttggaggt cactcacgcc gagcgtgaga
      421 atgctcaccc tcgattccac tgtccactct cgttctcttg gaaaccatag acgtatgaac
      481 tgtccaggag gaagcttcca tttcagcgcc aattcaccga agcctgcgga aggttctgcg
      541 agggccgtat ccggcatggt gaatcggatc ggaggacttc cgccctatga gagtgtatcg
      601 gctcacatta gatcagatcc ttgagtaacc tcaggtgggg cgcgagcaaa tcgagagcca
      661 gggctggcac gagggtgaag gtgagggtgc ccctcgacgg gagccctggc accaatcaac
      721 acgggatgct ttgtgctggt atgcacgccc ctgcacttca tgaccacgat gcttgccctg
      781 gtgctagcat acaacccaca actcatctac agcacgggct tccagctctc tgtggccgcc
      841 gtattcggga tccttctgct taccagaccc ctgaaatctc tcctggagag atctttgcta
      901 aggctcctca agagcccccc ggcgcacctg tcgaacctca tctccgtctc tctggcggca
      961 cagatcgcga catcacccat agtcgcagcc acattcgagc aggtctccat cgttggggtg
     1021 ttcacgaacc tcattgccgt gccgctttca gggccgatcc tgatcctcgg cctcctcggc
     1081 tctgtggccg gaaacatcta ccccctgatc gcctaccctc taaacgcctg caacggcttt
     1141 ctggtcacga tgctgataca gaccgctcag ggctcctctt cgctcccgtt cgcgtcggta
     1201 acgacaccgg gcgtaacgct tttcctcgtt ggcctgttct acgcaggatg cgtccccccg
     1261 atagtcgcag gacgtgtctt ctcgagcgaa cgcaagttcc tgtgggctgc tctcctccta
     1321 atctggtccg ccctctggtt ggtactcgtc gccgccggta gcatctgacc caacaccgtc
     1381 cccaaagcgt gagcacctcg cacaccgagc cacctgccaa caaagtgctg aagtgctgac
     1441 aaggcggcga agccgccgtg ctgacaagcg aaggccgaag gccggagcgt gctgaaatgc
     1501 tatcctggca aagccaactg gaccaagaga ggacaacatg agaagcaaac tggcgatggg
     1561 tggcctgacc ctgggcgcag cttggggagc ttatagtcgg ctacgcagac ccatcccgct
     1621 caaaggcagc tacgcgcgcg ccccgaccaa gatcctcatc atcggcggcg gattcggagg
     1681 cctctctgtg gcgagggggt tggcgcgagc gctccgcggg accgaagatg tgggcgtcgc
     1741 cctggtggat cgcatgaatt acaccacctt ctggccgatg gtgccttcgg tcatcccaag
     1801 caacgcggag gtgcgccatg tggcccactc cctcaggcgc atattgaagc ctttgggggt
     1861 agagttcttt caggatgagg tggtgggcgt ggacttcgag gctcgccggg tcaagacggc
     1921 tgagcgcgac tacccttatg actaccttgt cctggctccg ggcagccgca cgacctactt
     1981 cggcacccca ggcgcagaag agaatgccat ggatgtcaag ggtctgcgag acgccctgcg
     2041 ggttcgtaac cacgtgatcg actgctttga ggaagccgag cacctcgacg gccaggctcc
     2101 cgacgacctg ctcaccttcg tcgtcgtcgg cggaggtccc accggggtcg aggccgcggc
     2161 cgacatgcac gacctgatgt tcgacgtgct cgaggatgac tatcccaacg tcaagttcga
     2221 ccgtgtgcgc gtcgtgctgg ttaatgcagg cgaccagatc ctcaaaggtc tcgattcctc
     2281 actggtgaac gccgcaaggc gcaggctcgc cgctcagcga atagaggtca tcaacgacgc
     2341 caaggtcgaa gaggtcaggc ctgatgccgt ggtgatctcg ggcggtcgta caataccgac
     2401 tcgcaccacc gtttgggccg ccgggattga gccccctcct ctggtaggta acctggacgt
     2461 gcaaaaagac catcgcggaa ggatcctcat cgaccagtac ctgcaggtga agggccggcc
     2521 cggagtctat gccgtgggcg attgcacgag catacaatac gacggaccac cggtcccagc
     2581 ccttgcccag gccgcagaac aggagggcaa gagggcagcg gcgaacctcg tggcagagaa
     2641 caagaaccag gcacccgtag ccttccgtta ccgttccgta ggccagctgg tggatctcgg
     2701 ggagggtagc gccctagtcg acatccttgg agtaaagctg ggtggtctgc tcggggcgtt
     2761 catctggaag ggggtttacc tgtacgagct gggctacgac ctcaacaggg cacacgtact
     2821 cgccgactgg accttcgacc tgttcagccg cccagacacc tccaaactct tcgaagaatc
     2881 ccagcgagcc aagtacaaac aagacaacat agctcagcaa acaagtcccg aaacgtgaaa
     2941 tcctgacaag ccgaaatgct gacgagcgga ggccttgggg cccgagtgtg ctcacaggct
     3001 gaaaataccc catttgaggg atgcgccgcc ctattgcggg cgagtatgct gccattgggc
     3061 gatccgaccg agatacagat gtttggcacg atgtatggac cacaggtcat aaggagagcg
     3121 tgttgacacc gttgctctac cgtatgggac acttttgcgc ccgtcacctc gtgcgggtcc
     3181 ttgcactctg gctggtggtc gcactcacga tcgtgggtgt ggccaggatc gtcggccagg
     3241 agaccaacga cgacctctcg ctgccaggta ccgacagcca ggcggccagc gaccttcttc
     3301 gtgacaagtt ccctgagcag gccaacggct cggtcccgat cgctttccgg gctccggccg
     3361 gtgcaaaact ctccgatacc agatacaaga agccgataga acaggtcaca agtgcctttg
     3421 gcaaggactc tgcggtcacc cacgccgtcg gtccattcag cgagcagggt gccgaccaac
     3481 tcaacaagaa gcgtacgatc ggctacatct ccctcaacct gaaagacagc ccctcggagc
     3541 tcagcatcga ggaggcacaa cggatcatta gcctcgaccg cccactagag aaggccggcc
     3601 tgaacccggc cgccggcggc tatcttgggc agaaggtatc caagccatcg acgcacctct
     3661 ccgaggtaat tggcctgacg gctgccgtgc tcatcttgct ctttacgttt
//
LOCUS       DASTKM010000276         5448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_983585, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000276 DASTKM010000000
VERSION     DASTKM010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983585"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1501
                     /locus_tag="VFQ10_11140"
     CDS             266..1501
                     /locus_tag="VFQ10_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965939.1"
                     /translation="MERRAAWLGWSLVVLSVALLVGGIALSRAASSTVPDLSFRGETN
                     DASVFANLVTLLTFSVVGAIIASRHPRNTIGWLFCSVGVTIGLNSFAGDYVEFWLASG
                     FGKVSLAETAAWFSSWAWILLTYVPTTFVLLLFPDGRLPSHRWRPVAWVAVLGIAGVV
                     VGYALEAGPLEDFPQIANPYGVDAPIVRLVGVAGSIVAAGAMVASAVSLIVRLRHAGS
                     EKGQQIKWLAYGGAVAVGTLCVSLLINLWNGPVGIVVSNVALLGLPVFTGIAIVKHHL
                     YDIDLLINRTLVYGSLTTMLVAVYVSGIVLSQRVFVGLTGHEELPQLAVVASTLMIAA
                     LFAPLRRRIQAIIDRRFYRRKYDAAKMLSAFNARLRDETDLGTLSDDLVGVVKETMQP
                     EHVTLWLRPDTGSKGRQPH"
     gene            1677..2615
                     /locus_tag="VFQ10_11145"
     CDS             1677..2615
                     /locus_tag="VFQ10_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9965940.1"
                     /translation="MTKGSVTNGGSARRYAVLSIAAAVITIGLKLGAYYLTGSVGLFS
                     DAAESVVNLVAAVAALWALTFAVRPPDEEHAFGHSKAEYFSSGLESALIIIAAAWIGV
                     TAWGRLMDPQPLQNVGLGLSITLGAAVLNGGVALVLLRAGRRLRSITLRADAQHLLTD
                     VWTSAGVVLGVLMVQLTGWLVLDPLIGLVVAANIVWTGFRLLRDTAEGLLDRALPPED
                     QEVILRILSRYERQGIRFHALRTRAAGQRRFVSMHILVPGLWTVKQGHDLSEKIEEEV
                     AEALPQCTFFIHIEPSEDPASFEDQSLDRERRDGEA"
     gene            complement(2667..4985)
                     /locus_tag="VFQ10_11150"
     CDS             complement(2667..4985)
                     /locus_tag="VFQ10_11150"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET9965941.1"
                     /translation="MGMEVDRNRLADIDLTENARAVLGKRYLKKNENGEPIEEPIEMF
                     LRVASNIAEGEFRFKEGEEAKRLYEESRERFLQIMLSRRFMPNSPTLMNAGRELQQLS
                     ACFVLPVEDSIDGIYDTLKHQAVIHKSGGGTGFGFSRLRPKNDLVKSTMGVSSGPVSF
                     MAIYDASTDKIKQGGTRRGANMGILRVDHPDIEEFIDCKRDNDQINNFNISVAITDEF
                     MEAVEGDTDFELTNPRDGEIMKTVRARDLFRKIVEGAWLNGEPGVVFIDKINDDNPTP
                     QFPIESTNPCSEAHLPPYDSCNLGSINLERFYREDTGDVDWDDLRETVHTAIRFLDNV
                     IEQNNYPLPEIDEMSRGNRRVGLGVMGFADLLIKLGITYDSEEGLAFAERIMKFVDDE
                     AWEESRALAEERGVMPNYEGSRHQARGDRVRNATVTTIAPTGTISMIAGCSGGIEPLF
                     AVAFMRRQADMEMPDVNPEFVKLARERGFYSEELMKKIAEHGSVRDVEEVPDDVRSVW
                     VTSHDITSEWHVRMQAAFQKYTSMGISKTINLPNEATPEDVEDAYRQAYALNCKGIAV
                     YRDGSRDAQVLSTGKTGGEKPTMTPASPEAPTAPPVTPPAGASQEGQQQTFSSLAEAR
                     NGRPRTLTGVTKKIRTGHGSMYVTMNDDEFGPRECFVILGKPGGTAAAFSDALGRMIT
                     MALTHGATPAEVVHQLRGIQDGHPAGVGPNAVLSVPDGVARAMSENYLIEDSLPDTRE
                     LPIIGACPDCGSGLTRQEGCVVCHSCGYSKCS"
     gene            complement(5066..>5448)
                     /gene="nrdR"
                     /locus_tag="VFQ10_11155"
     CDS             complement(5066..>5448)
                     /gene="nrdR"
                     /locus_tag="VFQ10_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET9965942.1"
                     /translation="SRSCGKRFTTYERREPLRLMVIKRDGGKEPFDREKLRSGMAKAC
                     AKQQVTDEQVELIVDEIEAELRERRRHEVTSRRLGDMVLLRLRRMDMVAYLRFASVYR
                     QYTDVDQFRTELVRLAGSGMGNGR"
BASE COUNT          920 a         1660 c         1697 g         1171 t
ORIGIN      
        1 tccgggaagc agatcctgcc acaaccaccg ccaggtatcg ttgagctcgt gcgcttgatg
       61 ctcgtgtggg aaaagctgaa ttagcaaggg gcatcccatt gactctattc acacaccttg
      121 ctggaggatt ctggggaaca cgaccggccc taacgaataa ggccggtttc gtaacgcttg
      181 tcgtaacgct tgggagacgg tcgtcatgta gattggatgt gtggtgacag aaggggcaaa
      241 gggccaggat cgaggagttg gtaggatgga gcgacgagcc gcctggcttg gctggtcgct
      301 tgtggtgctt tccgttgcgc tgcttgtggg cgggatcgcg ttgtctcgcg cggcgagctc
      361 caccgttccg gatctctcgt tccgcggcga gacgaatgat gcaagcgtat tcgctaacct
      421 ggttacgctg ttaaccttct cggtcgtggg cgctataatc gcttcccgcc acccccgcaa
      481 caccataggt tggctcttct gcagcgtggg cgtgacgata ggcctcaact ccttcgcggg
      541 tgactacgtc gagttctggc tggcgagcgg ttttggcaaa gtgagcctgg ccgagacggc
      601 ggcgtggttc agttcatggg cgtggattct tctgacttat gtccccacga ccttcgtgct
      661 gctgttgttc cccgacggtc ggctaccttc gcaccgctgg cggcccgtgg cctgggtcgc
      721 ggtgctcggg atcgccggcg tagtggtggg ctacgcgctg gaggccggtc cgctcgaaga
      781 cttcccacag attgcgaacc cttatggcgt cgatgcgcct attgtgaggc tagtgggagt
      841 cgcaggtagc atagtagccg cgggggccat ggtagcctca gccgtctcac tcatcgttcg
      901 gctccggcac gccggtagcg agaaaggcca gcagatcaaa tggctcgctt acgggggtgc
      961 ggttgcggtt ggcaccctct gtgtgagtct cttgatcaac ctctggaacg ggccggtcgg
     1021 catcgtggtc agcaacgtcg ccctgctcgg gttgccggtc ttcacgggca tagccatcgt
     1081 caagcaccat ctctacgaca tagaccttct tataaaccgt actctggtct acggttcact
     1141 taccacgatg ttggtggcag tctacgtcag cggcatcgtc ctatcgcagc gtgttttcgt
     1201 cggactcacc ggccacgagg agctgccgca actggccgta gtcgcttcga ctttgatgat
     1261 cgcggctctt ttcgctcctc tgcgacggcg catccaggcg attatagaca ggcgtttcta
     1321 ccgcaggaag tatgatgcgg caaagatgct cagcgccttc aacgcccggt tacgtgacga
     1381 gacagatctg ggcaccctca gcgacgacct ggtaggggtg gtgaaggaga cgatgcaacc
     1441 tgagcacgtc acattgtggc tgcgtcccga cacggggtcc aagggccggc agccacacta
     1501 gcaacctccg aagctctatg ggccgaatac tacctgttgt ggtgtaggag tgggtcgcgg
     1561 ggtttgtgac tgagtttatc gggaagatgg tagaaagtgg aagtgtttgc tgcgatcccg
     1621 acattcattg aggctctcgc tgattcaagg tgcgcatctc cccgagtgag gcggcggtga
     1681 cgaaggggag cgtaacgaac ggcgggtctg cgcgccgcta cgctgtgctc tcgatagcgg
     1741 ctgctgtgat tactataggg ctcaaactcg gggcctatta tcttacgggg tccgtcggtc
     1801 tcttttccga cgcggccgag tctgtggtca acctcgtcgc cgcggtggcg gcgctgtggg
     1861 cgctgacctt cgccgtacgt ccgccggacg aggaacacgc cttcggccat agcaaggcgg
     1921 aatatttctc tagcgggttg gagagcgccc tgataatcat cgctgcggcc tggatcgggg
     1981 ttacggcgtg gggccggctg atggacccgc aaccacttca gaacgtgggg ctcggcctct
     2041 ccatcacgct cggcgccgca gtgctcaacg ggggcgtcgc gctcgtgctc ttgcgggcgg
     2101 gaaggaggct gcgatcgatc acgctcaggg ccgacgcgca gcacctgctc acggacgtgt
     2161 ggacctcagc aggcgtcgtt ctcggcgtct tgatggtgca gctcacaggg tggctcgtgc
     2221 tcgaccccct gatcgggctc gtcgtcgctg ctaacatcgt ctggacgggt ttccggcttc
     2281 tgcgcgacac cgcggaagga ttgctggatc gggccctgcc tccggaagac caggaagtca
     2341 tcttgagaat tctgtcccgt tacgagagac agggaatccg gttccacgcc ttacgcacgc
     2401 gtgcggccgg acaacgccgg ttcgtctcca tgcacattct cgtgccaggt ctgtggaccg
     2461 ttaagcaggg ccacgacctc tccgagaaga tagaagaaga agtcgccgag gcgttacccc
     2521 agtgcacctt cttcatccac atagaaccct cagaagaccc cgcgtctttc gaagatcaaa
     2581 gcctggatag ggagcgccgg gacggagagg cataagaaga gggacggacc gggcagatcc
     2641 gtccctggga ggtcgccgtt ggatttctag ctacacttcg agtacccgca ggagtggcag
     2701 acaacgcagc cctcctggcg ggtcaggccg ctgccgcagt cggggcaggc cccgatgatc
     2761 gggagttcgc gggtgtctgg caggctgtcc tcgatcaggt agttctcgga catggctctc
     2821 gccacaccgt cggggacgga gaggacggcg ttcggtccta cgccagcggg gtgaccgtcc
     2881 tggatgccgc ggagctggtg gaccacctct gcgggagtgg cgccgtgggt aagggccatc
     2941 gtgatcatac gccccagcgc atccgagaac gcggcggcgg tgccgcccgg cttgcccaat
     3001 atgacaaagc actccctcgg cccgaactcg tcgtcgttca tcgttacgta catcgagccg
     3061 tggcctgtac gtatcttctt ggtcacgccc gttagggtgc gcggccttcc gttgcgcgcc
     3121 tcggcgagcg aggagaaggt ctgctgctgc ccttcctggg acgcgcccgc cgggggagta
     3181 acggggggcg cggtcggcgc ctcgggggag gccggcgtca tggtcggctt ctccccgccc
     3241 gtcttgcccg tggacaggac ctgggcgtcg cgcgagccgt cgcggtagac tgctatccct
     3301 ttgcagttca gggcgtaagc ctggcggtag gcgtcttcta cgtcctcggg tgtcgcttcg
     3361 ttgggcaggt tgatggtctt ggagataccc atcgaggtgt acttctggaa ggcggcctgc
     3421 atcctgacgt gccactctga agttatgtcg tgggaggtga cccacacgct gcgcacgtcg
     3481 tcagggacct cttcgacgtc gcgcactgag ccgtgttctg cgatcttctt catcaactct
     3541 tcggagtaga agccgcgctc tctggcgagc ttcacgaact cggggttgac gtcgggcatc
     3601 tccatatcgg cctggcgacg catgaaggcc acggcgaaga gcggctcgat gccaccggag
     3661 cagccggcga tcatggagat cgttcctgtc ggggctatcg tggtgacggt cgcgttgcgg
     3721 accctgtcac cacgtgcctg gtggcgcgaa ccctcgtagt tgggcataac gccacgctcc
     3781 tcggcgaggg ccctgctctc ctcccaggcc tcgtcgtcca cgaacttcat tattctctcg
     3841 gcgaaggcga ggccctcttc cgagtcgtag gtgataccga gctttataag gaggtcagcg
     3901 aagcccatga ccccgagccc tacacgtcgg ttcccgcggc tcatctcgtc gatctcggga
     3961 agagggtagt tgttctgctc gataacgttg tccagaaagc ggatcgcggt gtgtactgtc
     4021 tctctcaggt cgtcccagtc gacgtctcct gtatcctccc ggtagaagcg ctccaggttg
     4081 atcgagccca ggttgcacga gtcataaggc ggcaagtgcg cctccgagca agggttcgtg
     4141 gactctatcg ggaactgtgg cgtcgggttg tcgtcgttga tcttgtctat gaagacgacg
     4201 ccaggctcac cgttgagcca agccccctcg acgatcttcc tgaacaggtc cctggcccga
     4261 acagtcttca tgatctcgcc gtcgcggggg ttcgtgagct cgaagtcggt gtcaccctcg
     4321 acggcctcca tgaactcgtc ggttatggcg acggagatat tgaagttgtt gatctggtcg
     4381 ttgtccctct tgcagtcgat gaactcctcg atgtcagggt ggtcgaccct gaggatgccc
     4441 atgttcgccc cgcgcctggt cccgccctgc ttgatcttgt ccgtcgaagc gtcgtatatg
     4501 gccatgaacg agacgggacc ggaagaaacg cccatcgtag acttcaccag gtcgttcttc
     4561 ggacgcaaac gagagaagcc gaagcccgtc ccgccgccgc tcttgtggat gacagcctgg
     4621 tgcttgaggg tgtcgtagat gccgtctata gagtcctcga cgggcaacac aaagcacgca
     4681 ctgagctgct gcagctccct gcccgcgttc atgagggtcg gcgagttcgg catgaacctc
     4741 cggctgagca tgatctggag gaacctctcc ctcgactcct cgtagagcct cttggcctct
     4801 tcaccctcct tgaagcggaa ctcaccctcg gcgatgttcg acgcaacccg caagaacatc
     4861 tcgatgggct cctctatagg ctcaccattc tcgttctttt tcaggtagcg cttccccaga
     4921 acggcgcgcg cgttctcggt gagatctatg tctgccagcc tgttacggtc tacctccata
     4981 cccatccttc ccattctact gctcgttgcc cttattccat caccggattc gcccgcttta
     5041 cccctgattg cgggcgctct ggtccctacc tgccgttgcc catacccgag ccggccagac
     5101 gcacgagctc ggtgcggaac tggtccacgt ccgtgtactg cctgtatacg gaggcgaacc
     5161 tcaggtaagc caccatgtcc atcctcctga gccgcaaaag caccatgtcc ccgagacgcc
     5221 tcgaggtgac ctcgtgccgc cgccgctcgc gcagctcggc ctcgatctca tcgacgatga
     5281 gctcgacctg ctcgtcagtg acctgctgct tggcacaggc cttcgccatc cccgaccgca
     5341 acttctcccg gtcgaatggc tccttcccgc cgtcgcgttt gatcaccatc agcctcaaag
     5401 gctcgcggcg ctcgtaagtc gtgaacctct tcccacacga gcgcgata
//
LOCUS       DASTKM010000277         9767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1511189, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000277 DASTKM010000000
VERSION     DASTKM010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511189"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFQ10_11160"
     CDS             <1..665
                     /locus_tag="VFQ10_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965943.1"
                     /translation="TPEERFKLYIEARARADGEECRNLEKSCPRLVYEMNVMAYEDRV
                     RASEMMTTLACLDLAPRLIKLRILMAFSSGLAALRNTCVDEAHSAYLRGRRLGGRARR
                     GAHSKDHPREVRGPDLGAQDDLGKITSHIEKEWAIFADQIGRVEQEMRVEVGVMWEAF
                     SSFAREEMGLEPKTLIKAWFEPILPEIEAVEDTLDTTETNPQRLQEYASMLRQLWSML
                     AP"
     gene            925..1188
                     /locus_tag="VFQ10_11165"
     CDS             925..1188
                     /locus_tag="VFQ10_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965944.1"
                     /translation="MHWTKCSSAGNPDKWPEAPEAVRKVTQAKDPEAALERISGGAEW
                     IPYDREALLRERGIRPRSLVAGRDVYDQEVEDLSEPEIREDGF"
     gene            complement(1355..2017)
                     /gene="lipB"
                     /locus_tag="VFQ10_11170"
     CDS             complement(1355..2017)
                     /gene="lipB"
                     /locus_tag="VFQ10_11170"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET9965945.1"
                     /translation="MTLQKRARSETTVDVRRLPGLTPYAEAWDLQKDLVQLRKKDGIP
                     DTFVLCEHPPVYTIGRAARDAGNLGAGEEYLRALGAEVFWSDRGGDATFHGPGQIVGY
                     PILRLEARDTHAYLRDLEGVIIRVLADYGLEGWHHPEYTGVWVGENKIAAIGVKFSSG
                     WITSHGFALNVKTDLSWFDRITPCGIKEFGITSLSRELSREVSLAEVEDKIVERFLRT
                     FR"
     gene            complement(2027..2947)
                     /gene="lipA"
                     /locus_tag="VFQ10_11175"
     CDS             complement(2027..2947)
                     /gene="lipA"
                     /locus_tag="VFQ10_11175"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET9965946.1"
                     /translation="MPFRQQARGVPGAHGRPEWLKARIPDGEVYRDIKETMRGLNLHT
                     VCEEARCPNIGECWNNRTATFMILGNVCTRSCGFCAVLTGKPTELDLDEPYRVADAAK
                     KMGLEHAVITSVNRDELADGGASIFAGTIHAIREVVPGCAVEVLTPDFKGDRDAVKTV
                     IDARPDTFNHNIETVPRLYFAVRPQAKYERSLEVLRYAKELNPNGLTKSGFMVGLGEV
                     EEEIQTTMRDLRDHDVDILTIGQYLRPSENHLPMSRYYTPKEFASLRKYGFSLGFKHV
                     ESGPLVRSSYHAHEQTADARRSPAAGVTSV"
     gene            complement(3251..4744)
                     /locus_tag="VFQ10_11180"
     CDS             complement(3251..4744)
                     /locus_tag="VFQ10_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET9965947.1"
                     /translation="MADQFDLVVIGGGNGGYIPAIRASQLGMSVALIEKREGGHLGGT
                     CLNLGCIPTKALLQTAHLLHDARNGEDYGVKVGEVEFDYPQAAKRREQVVNQLRRGVQ
                     GLMKKNKVTVYNGVGSFVEPKKIKVETEDGEEELEAENVLISTGSAVNTLPGLEFDHE
                     KIISSDDIVTNDDGYPESVIILGSGAVGVEFASMYNDFGTEVTIVEILDRLVPLEDPE
                     ISEALKKEFENRGITVLTSTKADPESLEKTDSGVKITVAGAESGEQEETEGEGGSYGG
                     EDPPTGEASEASGDTLEAEALLVAVGRKTVIEDLNLEATGVEVDERGIIQVDGYGRTA
                     EDGVYAAGDVVGGFWLAHEAGHEGIIAVEHMAGEDPMPKDRNLVPRVTFCRPEVASFG
                     LTKAQAEEEGYEVRETKFPFRAIGKALIEGEANGFFKIVADEETDLILGMHAIGPKVT
                     DLITEGVFAKLVEGTPEEIGMTMHPHPSLSEVVGEAAMAAEGHPIHI"
     gene            complement(4763..5626)
                     /gene="mutM"
                     /locus_tag="VFQ10_11185"
     CDS             complement(4763..5626)
                     /gene="mutM"
                     /locus_tag="VFQ10_11185"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET9965948.1"
                     /translation="MPELPEVETIKQDLRELVAGSEIVRARVLDASLVEQPSAEELVS
                     RLEGVSITGARRRAKHLIVELGSGDSLVFQLKIGGQLLLVPPVEEPESALMLVLDLDG
                     GRRLFLRDETGFTRARLLDADELQERLSPLGPEPLEEEFDAGYLQRAIGSRRAQIKPL
                     LLDQKVVSGIGNIYADEILYDARLHPRRKANTLSEGEWEALHAAIRSNLAAGVEHRGT
                     TVRLYKDVLDRPGEHQNYLRVFEKHGRPCPGCRGEVVREKVSGRPTHFCPACQREDSP
                     GGGGGLESLWQ"
     gene            complement(5670..6320)
                     /locus_tag="VFQ10_11190"
     CDS             complement(5670..6320)
                     /locus_tag="VFQ10_11190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9965949.1"
                     /translation="MEILDRNGIHHGLVVDLGCGSGLWARELVDAGYRVLGIDISEAM
                     IELSRNRVPEAEFRVGSLFEAEIPPCAAVTAVSEVLNYLFDAENEEQGLGRLFRRVHD
                     ALVPGGVFVFDVLGPGQVPPGTRARGFRVGEDWAVLAEREEDAEGGTMERRIVSFRKV
                     GECYRRDDEVHRVRLYEPAELSAELEQAGFQVRTMRSYGGHQLSEGHAAFVARKPA"
     gene            complement(6427..7161)
                     /locus_tag="VFQ10_11195"
     CDS             complement(6427..7161)
                     /locus_tag="VFQ10_11195"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET9965950.1"
                     /translation="MNPQEFLQRLKREVIGHPALTHPFLERFGEGDADAEGIRTFAIQ
                     YYRHVRVSRLYLAALISNCRDDEKLQLALAEVLFDEYGHLDPEETHPALYRRFLGALG
                     IDKEEWEEPPTLPEIEMYITTHYALSGHPDFRLGLGALGPASEWPVPPIYARLSDGLR
                     RAAGLPDEALEIFTSHVTMDVTHARIMMEAIAPYAGDEEGQRRVHEGAMRSLDARSVM
                     LGGLYRAVYREPVPLFDAPSVRLTGD"
     gene            7465..7995
                     /locus_tag="VFQ10_11200"
     CDS             7465..7995
                     /locus_tag="VFQ10_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965951.1"
                     /translation="MTDTKNNIRQDWLSVPRRREEERLDPHVYKRGINGLTVEEALRR
                     AFPRVIDILGRSGEQYSEFELQLVRQTVRACLISQVSRANLYRIAGILVADRRDEGAH
                     IDTSLALAYVAALREAFLIELSVGERVQTPLGSGEVRYVSGDGANAAVKLDNDYEVWL
                     PAESVSRPKALVRRAS"
     gene            8067..8987
                     /locus_tag="VFQ10_11205"
     CDS             8067..8987
                     /locus_tag="VFQ10_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9965952.1"
                     /translation="MEKSRARVICNPTSGGGSYDPDEIRDELESLEVEWVQTEGPDDA
                     IGAAEEWREGLLIVAGGDGTINDVVNGLGRADFPEGVTLGILPAGTGNDLAATLCIPE
                     DPGLAEDVIRQNRQRRLDVARVRSEGIGERFFINVATGGLGAEISDANDEELKKRWGK
                     LSYLRASLEVARDFDVRDLTLYLDGEERRVKAVNIAVGNCRYTGGGWPATPKANPEDG
                     LLDVVVIEALGVADLLELAPAALSESDYLDSDGVLFVRAKEIRIETQPPGLEFTADGE
                     IIGNEPARFSILPQALNVLVGPEYVSEPAG"
     gene            8997..9239
                     /gene="moaD"
                     /locus_tag="VFQ10_11210"
     CDS             8997..9239
                     /gene="moaD"
                     /locus_tag="VFQ10_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HET9965953.1"
                     /translation="MIKVLLFGAAADRAGTREIELPVDEGVTLAEIWPLLARRHPDLT
                     PMRDTLAFAVNGEYARGDASVSPGDEVAVLPPVSGG"
     gene            9240..9683
                     /locus_tag="VFQ10_11215"
     CDS             9240..9683
                     /locus_tag="VFQ10_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET9965954.1"
                     /translation="MFAIVEEVLDVGALIQDAQRPDCGAIATFVGTTRIDESEGETVE
                     YLEYEAYRPMADRKLEEIGAEIEGRWDVRHVSIVHRLGRVDPGEASVAIVVAAPRRGP
                     ALEASRYAIERIKEVVPIWKREVWSDGYVWVGSQVGTRNVASGKP"
BASE COUNT         1709 a         3046 c         3089 g         1923 t
ORIGIN      
        1 ttaccccgga ggagcgtttc aagctctata tcgaggccag agcacgcgcg gacggggagg
       61 aatgccgaaa cctggagaaa agttgtccga gactggtcta cgagatgaat gttatggcct
      121 acgaagatcg ggtgagggct agcgagatga tgacgacttt ggcgtgcctg gatctggccc
      181 cccgcctcat caaactgagg atacttatgg ccttctccag tggtttggcc gcgctgcgca
      241 acacgtgtgt ggacgaggcg cactcggctt acctccgcgg taggaggctg ggagggaggg
      301 ctcgtagggg cgcccactcg aaggatcacc cacgggaggt gcggggcccc gacctcgggg
      361 cgcaagatga cctaggcaag ataacatctc acatagagaa agaatgggcc attttcgccg
      421 accaaatcgg tcgggtcgag caggagatgc gagtggaagt cggggtcatg tgggaggcgt
      481 tctcgagttt cgcccgcgaa gaaatgggat tggagcccaa aacgctgatc aaggcatggt
      541 tcgagcccat cttgcccgag atcgaagcgg ttgaggacac cctggatact acggagacga
      601 acccgcagag gctccaggaa tacgcctcaa tgctccggca actctggagc atgctagcac
      661 cttgatcgtg ggctcgttac ccggtctatg ttctcctgcc gggcgggtca ggcaccagag
      721 aatgcgttcg cagaggatgc gttctagtgg atgttgttcg ttgtttccgg gacgatccct
      781 tggactaacg gaacgtccgg agaaagcgct cgacgatctt ctcttcgact tcggcgaggg
      841 agacgtcgtg gtcgagttcc cggctcaggc tcgttatgcc gaactcctcg cttctactac
      901 aacccgcaaa ccctggagct gtttatgcat tggacgaagt gttcgtcggc gggaaacccc
      961 gacaagtggc cagaggcgcc cgaagcggtg cgtaaggtaa cgcaggccaa ggaccccgaa
     1021 gcggcgcttg agcgcatcag cggcggagcc gagtggatcc cctacgaccg ggaggcgctg
     1081 ctgagggagc ggggcatcag gccgcgtagc ctcgttgcag gccgggacgt ctacgaccag
     1141 gaggtagagg acctctccga gcctgagatc cgcgaggatg gcttctgagg gcctccgtgc
     1201 cccggtccag ttcgcccctt gcctctgatc ctcgcgctgc tgtaggctgt ccctgggggg
     1261 aggactgacg cggggagggc gctgtgcttg acgagagggt cacgctgcga gaggctcctc
     1321 ttcggttggg cgtatccgag ggagccatct tgaactaacg gaacgtccgg agaaagcgct
     1381 cgacgatctt gtcttccact tcggcgaggg agacctcgcg actcaattcc cggctcaggc
     1441 tggtgatgcc gaactccttt atgccgcagg gtgtgatgcg gtcgaaccag ctcaggtcgg
     1501 tcttcacgtt cagggcgaag ccgtgggagg tgatccaacc agacgagaac ttcacgccta
     1561 tggcggcgat cttgttctcc cctacccaca cacccgtgta ttctggatgg tgccagccct
     1621 cgagcccgta gtctgcgagc acccggatga tgacgccttc gaggtctcgg aggtaggcgt
     1681 gggtgtcgcg ggcctcgagt cgcaggatcg ggtaccccac gatctgacca ggaccgtgga
     1741 aggtggcgtc ccctcccctg tcgctccaga aaacctcggc ccccagagcc ctgaggtatt
     1801 cttctccggc tcccaggttc cccgcgtccc tggccgccct tccaatcgtg tagaccggcg
     1861 ggtgctcgca caggacgaaa gtgtcgggga tcccatcttt cttcctgagc tgtactagat
     1921 ctttctggag atcccaggcc tccgcgtagg gtgtcaatcc aggcaaccgc cgcacatcga
     1981 cggtcgtttc ggatcgggcc cgcttctgta gggtcatgcc cgacctctag acgctcgtta
     2041 cgccggccgc agggctccgc ctggcatcgg cagtctgttc gtgggcgtgg taggagctac
     2101 ggaccagcgg cccgctctcg acgtgcttga agcccagtga gaagccgtac ttgcgcagac
     2161 tcgcgaactc tttaggcgtg tagtagcggc tcatcggcag atggttctcg gagggcctga
     2221 ggtactggcc gatggtcagg atgtccacgt cgtgatcgcg caggtcgcgc atcgtagttt
     2281 ggatctcctc ttcgacttcg ccgagaccga ccataaagcc gctcttggtc aggccgttag
     2341 ggttcagctc cttggcgtaa cgcagcacct ccagggagcg ttcgtacttc gcctgcggac
     2401 ggaccgcaaa gtagaggcgg ggtaccgtct cgatgttgtg gttgaaggtg tcgggacggg
     2461 cgtctatgac ggtcttgacc gcgtcgcgat cgcccttgaa atcgggggtc aggacctcga
     2521 cggcacagcc ggggaccact tccctgatag catgtatcgt cccagcgaag atcgaagccc
     2581 cgccgtcagc gagctcgtcg cggttcacgc tcgtgatcac ggcgtgctcc aggcccatct
     2641 tcttcgcggc gtcggcgacg cggtagggct cgtcgaggtc gagctcggtc ggtttgcccg
     2701 ttagcacggc gcagaagccg catgaacggg tacagacgtt gcctaggatc ataaaagtgg
     2761 cggtgcggtt gttccagcac tcgccgatgt tggggcagcg ggcctcctcg cagacggtgt
     2821 ggaggttcag gccgcgcatg gtctccttga tgtcgcggta gacctcgccg tctgggatgc
     2881 gcgccttgag ccattcagga cgaccgtgcg cgccagggac tcccctcgcc tgctgcctga
     2941 agggcaggta ctccgtcggc gtcccgtcgg gcggcggggg cactggcctg tcttcccagc
     3001 ggtggcggac ctcgaaggtc ttcgtaccat cgtccaatac cttcaggttg atctgtctgc
     3061 gggccacagc gctcctatct tcttcgcatt ctgcgcctat ggtatcgcat ggaggcagac
     3121 ccgtgccgtg tcacctatct cagagcggaa tgggaagggc cgaggccttc ccattccgtc
     3181 ctacaggtct caggaagtgc acgagagcga cgtggcccca aaaccccacc tgacggctga
     3241 tgcctgaaac ctatatatga atcgggtgtc cttccgcggc catcgcggct tctccgacga
     3301 cctcggagag gctcgggtgg ggatgcatgg tcatcccgat ttcttccggc gtgccctcga
     3361 cgagcttggc aaagacgccc tcggtgatga ggtccgtaac cttcggccct atggcgtgca
     3421 tcccgaggat cagatctgtc tcctcgtcgg cgacgatttt gaagaacccg ttggcctcgc
     3481 cctcgataag cgccttgcct atagccctga acgggaactt cgtctccctg acctcgtagc
     3541 cttcctcctc ggcctgcgcc ttggtgaggc cgaaagacgc cacctcgggc ctacagaagg
     3601 ttactcgcgg gacgaggttc ctgtccttgg gcatcgggtc ctcgccggcc atgtgctcga
     3661 cggcgatgat gccttcgtgt ccggcctcgt gggcgagcca gaaacccccg actacgtccc
     3721 ctgccgcgta cacgccgtcc tcggccgtcc gcccgtaccc gtccacctgg atgatgcctc
     3781 gctcatcgac ctcgacgccg gtcgcctcca ggttgaggtc ctcgataacg gtcttgcgcc
     3841 cgacggcgac gagcaaggcc tcggcctcca gggtgtcgcc actagcttcg ctggcctcac
     3901 cggtgggcgg gtcttcgccg ccataagagc cgccttcgcc ttccgtctcc tcctgctcgc
     3961 ctgattctgc gccggccacg gtgatcttga cgccgctgtc ggtcttctcc aggctctcgg
     4021 gatcggcctt ggtactcgtg agcaccgtaa tgccgcgatt ctcgaactcc ttcttgagcg
     4081 cctcggagat ctccggatct tcgagcggta cgagacggtc caggatctcg acgatggtca
     4141 cctcggtgcc gaagtcgttg tacatgctcg cgaactcgac gccgacggca cccgagccga
     4201 ggatgatcac ggactccggg tagccatcgt cgttggtgac gatgtcgtct gaggagatga
     4261 tcttctcatg gtcgaactcc aggcctggca gggtgttgac ggccgagccg gtggagatca
     4321 ggacgttctc cgcttcgagc tcctcctcgc cgtcttcggt ctccaccttt atcttcttgg
     4381 gttcgacgaa gctgcctaca ccgttgtaga cggtgacctt gttcttcttc atcaggccct
     4441 gcacaccgcg cctgagctgg ttgacgacct gttcgcgacg cttggccgcc tgaggatagt
     4501 cgaactctac ctcgccgacc ttgaccccgt agtcttcgcc gtttctggcg tcgtgcaata
     4561 gatgggccgt ctggagcaaa gctttggtcg ggatacagcc caagttaagg cacgtccctc
     4621 cgagatgccc gccctcccgc ttctcgatga gggctacgct catccccagc tggctggccc
     4681 ggatcgcagg tatatatcca ccgttcccgc cgccgattac cacgaggtcg aactgatcag
     4741 ccacccttgg ctcctttcgc gtctactgcc acaacgattc taaccctccc cctcctccgg
     4801 ggctatcctc ccgctggcaa gcaggacaga agtgcgtcgg tctcccgctt accttttccc
     4861 gaaccacctc tcctctacac cccggacacg gccgcccgtg cttctcgaag acccgcaggt
     4921 agttctgatg ctcgcctggc cggtccaaga catccttgta gagccgcact gtggtccccc
     4981 gatgctccac gccggccgcg aggttggacc ggatcgccgc atgtagcgcc tcccattcgc
     5041 cctccgaaag cgtgtttgcc ttgcgcctcg gatgcagcct cgcatcgtac aggatttcat
     5101 cggcgtagat gtttcctatg ccggagacga ctttctgatc caggagcaag ggctttatct
     5161 gggctctccg cgaaccgatg gcacgttgca gatatcccgc gtcgaactct tcctctaggg
     5221 gctctggacc aagcggggag agccgctcct ggagttcgtc agcgtcgagc aggcgcgctc
     5281 gtgtgaagcc cgtctcgtcg cgcaggaaga gcctcctgcc gccgtcgaga tcgaggacga
     5341 gcatgagcgc ggactcaggc tcctcgacgg gcggcacgag aaggagctgg cctccgatct
     5401 tgagctgaaa caccagcgag tctccggagc caagctctac gatgaggtgt ttggcccgcc
     5461 gccgcgcccc tgtgatgcta accccttcga gccggcttac taactcttct gccgaaggtt
     5521 gctccacgag cgaagcgtcg aggactcgtg cccgcactat ctctgagccg gcgaccagct
     5581 cgcgcaggtc ttgttttatg gtctcgacct ctggcagctc cggcatacgc gtatttatac
     5641 ccgctcggcg ctgccctatt cttcctgact catgccggtt tgcgtgcgac gaaggcggcg
     5701 tggccctccg acagctggtg cccaccgtaa gatcgcatcg tccgaacctg gaagcccgcc
     5761 tgttcgagtt ctgcagacaa ctccgccggt tcgtagagcc gcacacgatg cacctcgtca
     5821 tcccgcctgt agcactcccc caccttccgg aaactcacga tccggcgctc catcgtaccc
     5881 ccctcggcat cctcctcccg ctcggcgagt accgcccagt cctcccccac gcggaagccc
     5941 ctcgccctgg tcccgggcgg cacctggcca ggaccaagca cgtcgaagac gaagacaccg
     6001 ccggggacga gcgcgtcgtg aacgcgccgg aagaggcggc caagcccctg ctcctcgttc
     6061 tccgcgtcga aaaggtagtt gaggacctcg cttacggctg tgacggcggc gcaaggcggg
     6121 atctcggcct cgaacagcga ccctaccctg aactcggcct ccggcactct attgcgcgag
     6181 agctcgatca tggcctcgga gatatcgatc cccaagactc tgtagccggc gtctactagc
     6241 tcgcgcgccc acagaccgct accgcagccg aggtcgacaa cgaggccatg gtgtatcccg
     6301 ttacggtcca ggatctccat tatgcccggc gccgctccga gggcgaactc agcgtggccg
     6361 acgtcgtgga tgtacgccag gtcctccccg taccaccgcg gcgtgtcagg gccccccgag
     6421 gttatctcag tcccctgtaa gccgtacgga gggagcgtcg aacaggggga cgggttccct
     6481 gtagacagcc ctgtacagac cgccgagcat gacggagcgg gcatcgagcg agcgcatggc
     6541 gccctcatgg actcttcgtt ggccttcctc atcccctgcg tagggcgcga tggcttccat
     6601 catgatgcgg gcgtgggtca catccatcgt gacgtggctc gtgaatatct ccagcgcttc
     6661 gtcaggcagg ccggccgccc tcctgaggcc gtcggagagc ctggcgtaga tcgggggcac
     6721 gggccactcg ctcgccggcc ccagtgcacc gagcccgagc ctgaagtcag ggtggcccga
     6781 gagcgcatag tgggtggtga tgtacatctc tatctcgggc agcgtcggag gctcctccca
     6841 ctcctcctta tcgatgccaa gcgcccccag gaaacgcctg tacagggctg gatgcgtctc
     6901 ttcggggtcg aggtgtccat actcgtcgaa tagaacctcg gcgagcgcga gctggagctt
     6961 ctcgtcgtcg cggcagttgg agataagtgc ggccaggtac aaccggctca ccctgacgtg
     7021 cctgtagtac tggatcgcaa acgtccttat cccctccgca tcagcatccc cctccccgaa
     7081 ccgctccagg aaagggtgcg tgagcgcagg atgcccgatg acctcacgtt ttagccgctg
     7141 aagaaactct tgcgggttca cctgtctctc cttccagagt actccctaca gtttagcgta
     7201 aggttcgagc cgatcagcat ttcagcacgc tccggcctta cggcctccgc ttgtcagcat
     7261 ttcagcatgt caggttttgc ggtaggagat gactctcaaa gagtacgtgc tcctgcattc
     7321 tctaaagcgc aaaagaatct caccaccctt gacattgctg agtggctgaa aagccgccag
     7381 gtttcgccag gcggctcgct gaaatgctga ctttacaggc gatttaccgt tgtctcacta
     7441 gtctcagtga ggtataatag caacatgaca gatacgaaga acaacatcag gcaggattgg
     7501 ctcagtgtcc cgcggcggcg ggaggaggag cgtctggacc cgcacgtgta caagcgcggc
     7561 atcaacgggc taacggtgga ggaagcgtta cgtcgggcgt ttcccagggt gatagacatt
     7621 ctggggagga gtggcgagca gtattcggag ttcgagctgc agttggtaag gcagaccgtt
     7681 agggcttgtc tgatctccca ggtctcccgc gcgaacctct accgtatcgc cggcatcctg
     7741 gttgccgaca ggcgcgacga gggtgcgcac atcgacactt cgctcgcgct cgcctatgtt
     7801 gcggcgctca gggaggcgtt cctcatcgag ctctccgtcg gcgagcgggt acagacgccg
     7861 ctcggttccg gggaggtccg ctacgtctcc ggtgacggcg caaatgccgc cgtcaagcta
     7921 gacaacgact acgaggtctg gttgccggcc gagagtgtgt cgcgccccaa agcgctggtt
     7981 cggcgcgctt cctagggagg accctgtcat agacaggggc tttacgggta gactaggagg
     8041 accgtaaggg ctaccgaggg cgcgttttgg agaagtcgag agcacgggta atctgcaacc
     8101 ctacctccgg tggcggctcc tacgaccctg atgagatccg tgacgagctc gagagcctgg
     8161 aggtcgagtg ggtccagacc gagggccctg acgatgcgat tggggctgct gaagagtggc
     8221 gcgagggcct gctcatcgtc gccggggggg acggcaccat aaatgacgtg gtgaacgggc
     8281 ttggcagggc tgatttcccc gaaggcgtca ccctgggtat ccttcccgcc gggaccggca
     8341 acgatctcgc cgcgacgctg tgcatcccag aggaccctgg gctcgcggag gacgtcatcc
     8401 ggcagaaccg gcagcggagg ctggacgtgg cgagggttcg ttcggaggga attggcgagc
     8461 gtttctttat caacgtggcg acgggcgggc ttggggcgga gatctccgac gctaacgacg
     8521 aagagttgaa gaagcggtgg ggcaagctct cctacttgcg ggcttcgttg gaggtcgcaa
     8581 gagacttcga cgtgcgcgac cttacgctct acctcgacgg tgaggagcgt cgagtcaagg
     8641 cggtcaacat cgccgtgggc aactgccgct acacgggagg cggctggccg gctactccca
     8701 aggccaaccc tgaagatggt ctactcgacg ttgtcgtcat agaggcccta ggagtggctg
     8761 atctgctcga actcgcgccg gcggcgctgt cggagtcgga ctatctggac agcgatggcg
     8821 tgttattcgt gcgggcgaag gagatccgga tcgagaccca gcccccgggc ctggagttta
     8881 ctgccgacgg tgagatcatc ggcaacgaac cggcccggtt ttcgatcctt ccccaggctc
     8941 tgaatgtcct ggtcggaccc gagtacgtct ccgagccggc gggatagaat agagccatga
     9001 tcaaggtgct actcttcggc gccgcggccg acagggccgg gactcgcgag atcgagttgc
     9061 cggtggacga aggcgtgacc ctcgcggaga tctggcctct gctcgcaagg cgacatcccg
     9121 atctcacccc gatgcgcgac acgctcgcct tcgccgtcaa cggcgagtac gcgcgcgggg
     9181 atgccagcgt ctcgccaggt gacgaggtgg cggttttgcc gcccgtctca ggcggttgaa
     9241 tgttcgccat agtcgaggag gtgctagacg tcggggcttt gatccaggac gcgcagaggc
     9301 ctgactgtgg cgccatagcc acgttcgtcg gcacgacgag gatcgacgag tcggagggcg
     9361 aaacggtgga gtacctggag tacgaggcgt accgccccat ggccgacagg aagctggagg
     9421 agatcggggc ggagatagaa gggcgctggg acgtcaggca cgtctcgata gtgcaccgtc
     9481 tcggcagggt tgatcccggc gaggccagcg tcgccatagt cgtcgccgct ccgcgtcgcg
     9541 gtccagcgtt ggaggccagc cgctacgcca tcgagcgtat aaaggaggtg gtgcccatct
     9601 ggaagcgtga ggtctggtca gacgggtacg tttgggtggg aagccaggtc ggaaccagga
     9661 acgtggcctc cgggaagcct tgatattcgg cttcagattt ctctggcggg ggctacgtga
     9721 tgagcacgta tatgacgaac gccccggcga aggccaggct acctgcg
//
LOCUS       DASTKM010000278         5953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1161957, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000278 DASTKM010000000
VERSION     DASTKM010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161957"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFQ10_11220"
     CDS             <1..386
                     /locus_tag="VFQ10_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9965955.1"
                     /translation="NLVDLNRRMARGRYELELLVEDDFPARLPNKAGQELTRLVQEAL
                     TNVRRHAKANHVRVELGLDGGLAYVEVSDDGCGFDSERATTGMGRQSMRHRALELGGE
                     FSVESAPGEGTRVRFSIPVTRLVKG"
     gene            468..1157
                     /locus_tag="VFQ10_11225"
     CDS             468..1157
                     /locus_tag="VFQ10_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9965956.1"
                     /translation="MAIIENRAREYSTRILLVEDHASFRQALAFMFEREQEFIVTGQA
                     GSLAEARAFMRKVPDGVDVAVVDLALPDGDGFELIEELASRPDVMTLVLSASLEPARF
                     AKAVEAGAAGVLHKSAAIGEIVEAVRRLKSGEALLSPAEVIEMLRMVSRKRQEEYEAQ
                     RAIEKLTPREKQVLQALGEGLDSKDIAEKLHITVETERTHMVNILNKLDVHSRLQALV
                     FAARHGLVEIR"
     gene            1373..1687
                     /locus_tag="VFQ10_11230"
     CDS             1373..1687
                     /locus_tag="VFQ10_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965957.1"
                     /translation="MMSNEPRAYREGIAAVISQLRPEAEIETVEPEALDTSIERFSPD
                     MVICSKATEALKGSVRVWVELYPENAALSVASIDGRRMEYAEIQLPDLLSIVDKAEEL
                     AN"
     gene            1759..2079
                     /locus_tag="VFQ10_11235"
     CDS             1759..2079
                     /locus_tag="VFQ10_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965958.1"
                     /translation="MGTRRILVANELTSYRQTIAIVFRELHPNVEVLEVDPGTLDWEV
                     ARLGPALVICSRLTGVVEERAANWIELYPECRPFSTFCIDGERRAMEDVQLSDLLSVC
                     ARVL"
     gene            complement(2066..2788)
                     /locus_tag="VFQ10_11240"
     CDS             complement(2066..2788)
                     /locus_tag="VFQ10_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965959.1"
                     /translation="MAEREFRLPGSPYEELVNIIVAYGTRDEAARAGDVGKLDSVHQS
                     SVSRNNAFLTEIGVLQGESKKLITRRGRSLAVALARKDNAEVRSNWRAIVAASEFLQN
                     VVSAVKLREGMLYPTVQAYIAHAAGQPRNKPVMNGAGAIIEILKASGMLKEEAGELVA
                     TFDEGPEDIAPEDGSPAKTSEWKESVVSATVGEAPGASADVPAGTPPTVSIHVQVRCT
                     ADEIEDLAPRLKALLRELSTEP"
     gene            complement(2869..4077)
                     /locus_tag="VFQ10_11245"
     CDS             complement(2869..4077)
                     /locus_tag="VFQ10_11245"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HET9965960.1"
                     /translation="MEYEKDSRESTGVAFVLAVYAASRLFYLISGSLLAYLVPTSSFQ
                     SVTLDVPSGSMNIWSHWDGEHYVTLAMGGYLHPPDNVSPAFFPLYPLLLRSFAELLGG
                     PITKEALSVLAPLLSLVFLPFALYFIYYIALDAFDERVAKGTVLILAFFPTTFFLNAA
                     YTESLFLALSAGSLWAIRVRKDLLVACVLAALAAATRNVGVFLVVPLMYEWIKDVERF
                     RWRGIYLLLAPGGLFAYMGYLWVRFGDPLLFYSAQESWGRQATGPLDTASRAWASAVE
                     GAGRLLGHDLWAHPTLSNVANQLAGASNFFNLAFFVFAVVVLLAGSRYLPLSLTVYGL
                     LLVAPATLFGTPGSPLMGTPRYVLVAFPVFIVLALLFKNRLLLGGWLVMSTLISLALC
                     GLFVSWRFVA"
     gene            complement(4211..>5953)
                     /locus_tag="VFQ10_11250"
     CDS             complement(4211..>5953)
                     /locus_tag="VFQ10_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965961.1"
                     /translation="APLAAALALALLMFLGIAPGEWLMIPIAVLVLAAGIAYWATLQG
                     RDASPAEPGRSFPAAGRYALLSLVILLVLARGYLGPIVNGWSYPRGVDRYEHAVMAGM
                     MMREGSAESFMLYPPGFHLLTAMISRLSGIEPLEVFPILAPMLPLMGALACYALAKRL
                     WGWEYGVAAALTSGLLLGGTYLHFEEARYPNFIGEYFLIVLAVAALVGMYASPSARAG
                     LLLALLGSSTVLYHQIAGYSLAVLLGLVCVLFLPYLLLRDRRKGVYLALSLALLGLFS
                     VIYAWDTYDLPSLVAGMLGGSETGRGGEAVAMAIGTKPPNGPGHFLTTMSHPVLWLGL
                     LGLSLMLAGGKNARDTPGILARLTLLVWTLLLFFGSLTSYSGFPDRFERDLGVPLALL
                     AALTLVTLVRLSPRSGGRTVYMAALLAVTFSVTLVGAQSVLSLEQAAGPSTRPKDRPP
                     PAEVVAAGVWLKQHNDGGSIVATPYLKHVPSRAMLAMGGYTRMQSYDKKRIRRARDLP
                     PFGAPPLWDALWVLEHPAGGRTAQILEKNDVHYIVFHKRYPWIDWRPFALQRDLYRTV
                     YENDSVIIFAPRQN"
BASE COUNT         1251 a         1789 c         1909 g         1004 t
ORIGIN      
        1 agaacctcgt ggatctgaac cggcgaatgg cccgtggaag gtacgaactc gaactgttgg
       61 tagaggacga ttttccggcc aggctcccga acaaggcagg ccaggagctc acgcgcctcg
      121 tccaggaggc gctcacgaac gtccgacgcc acgccaaggc caaccacgtt agggtagagt
      181 tgggtctcga cggagggctc gcctacgtag aggtctccga cgacggatgt gggttcgact
      241 cagagagggc cacgaccggt atgggcaggc agtccatgag gcaccgggct ctcgagctcg
      301 gcggggagtt tagcgtggag agcgcgcctg gcgaggggac gagggtgcgc ttctccatac
      361 ccgtcacccg cctggtcaaa gggtagcggc gccttcctgc gtcgaacgct atctcagccc
      421 tttcgggctg aggtaagatg gtgcacaggc atcgaagcgt tagaagggtg gctatcatag
      481 agaatcgcgc tagggagtat tcgacccgga tcttgctcgt cgaggaccac gcatcgttcc
      541 ggcaggcgct ggccttcatg ttcgagcggg agcaggagtt catcgtgacg ggccaggcag
      601 gctcgctggc cgaggcgcgt gcgttcatgc gcaaggtacc cgacggggtg gacgtcgcgg
      661 tcgtagacct tgcgttgcct gacggagatg ggttcgagct gatcgaagag ctggcttccc
      721 gtcccgacgt aatgacgctg gtccttagcg ccagcctgga gccggcccgc ttcgccaagg
      781 ccgtcgaggc aggcgccgcc ggcgtgctgc acaagtctgc cgcgataggc gagatcgttg
      841 aggccgtgcg tcggctcaag tccggggagg ctctgctctc gccggcggag gtcatagaga
      901 tgctgcgaat ggtcagccgc aagcgccagg aggagtacga agcccagagg gccatagaga
      961 agcttactcc ccgcgaaaaa caggtgcttc aggctctcgg cgagggcctc gacagcaagg
     1021 acatcgcgga gaagctgcac atcaccgtag agaccgaacg cacccacatg gtcaacatac
     1081 tcaacaagct cgatgtccac tccagactac aggctctggt gttcgccgcc cgccacggcc
     1141 ttgtagagat ccgttaggcg cccgtggccc tcacgacaga accagccgaa cttttgtgag
     1201 gcgcaccgtg ctagaatcag aggtgctcgt gtcgatgacc gcggagacag attcgaagca
     1261 cgaagctacc cgaggagaca cgaacttctt gccgaagtca ccccataacc gctccaaaga
     1321 ccctgcagcg gaggcggcct ctgcgcggcg ccgaggaggt aagccgcgga tcttgatgtc
     1381 caacgagccg cgcgcctacc gcgagggcat agcagcggtc atcagccagc tacgccccga
     1441 ggccgagata gagaccgtag aaccggaggc tctcgatact tccatcgagc gcttctcacc
     1501 cgacatggtc atatgcagca aggccacgga ggcgttgaaa ggcagtgtgc gggtgtgggt
     1561 agaactctac cccgagaatg ccgcgctgtc ggtggcgagc atagacggca gacgcatgga
     1621 gtacgccgag atccagctac ccgatctact ctccatcgtg gacaaggccg aggagctggc
     1681 gaactagccc aatggggcta gaaaaacata cccccttttc gtgatgctga gtgtccttcc
     1741 agaggtcacc attacggtat gggcacaaga cgcatactcg tggccaacga acttacttcg
     1801 tatcgtcaga cgatcgccat cgttttcagg gagctacatc cgaacgtgga ggtgctggag
     1861 gtcgaccccg ggaccctgga ctgggaggtg gcgcgccttg gtcccgcctt ggtgatctgc
     1921 agccgcctaa ccggtgtggt cgaagagagg gccgcaaact ggatcgaact ctaccccgag
     1981 tgcaggccct tctcgacgtt ctgcatcgac ggagaacgca gggcgatgga agacgtccaa
     2041 ctctctgacc tcctctcggt gtgcgctagg gttctgtaga gagctctctg agcagcgcct
     2101 tcagccgcgg ggcgagatcc tcgatctcat cagcagtaca tctaacctgg acgtgaatgc
     2161 tgaccgtagg tggtgtgcct gcagggacat ccgcggatgc ccctggggcc tcgcccacgg
     2221 tagcggagac cacggattct ttccactcgg aagtctttgc gggcgaaccg tcctcagggg
     2281 cgatgtcctc cggtccctcg tcgaaggtgg cgaccagctc tccggcctcc tctttcagca
     2341 tacccgaggc cttcagtatc tcgattatgg cgccggcgcc gttcatcacg ggtttgttac
     2401 ggggctggcc agcggcgtgc gctatgtagg cctggacggt ggggtagagc attccctcgc
     2461 gcagcttcac ggcagagaca acattctgca agaactcgct ggccgcgact atcgcgcgcc
     2521 agttgctcct gacctctgcg ttatccttgc gcgctagagc cacggccaga gatcgtccgc
     2581 ggcgtgtgat cagcttcttg ctctctccct gtagaacccc gatctccgtg aggaaagcgt
     2641 tgttgcggct gacgctcgac tgatgcaccg agtctagctt gcccacgtcc cctgctctcg
     2701 ccgcctcgtc gcgggttccg tacgccacga ttatgttgac tagctcctcg tagggagagc
     2761 caggaagcct gaactccctc tccgccatcc cgacacctcc tccccccaag acgtttccca
     2821 aagaactcgt tcgggccatt atagaggacg ccgcgacgga gagggagttc aggctacgaa
     2881 acgccagctc acgaagagtc cgcacagggc aagtgatatg agggtgctca tgaccagcca
     2941 tccgccgagg agcaatctgt tcttgaacag gagcgcgagc actatgaaaa cgggaaatgc
     3001 gacgagcacg taccgtgggg tgcccatcag cgggctcccc ggtgtcccga agagcgtggc
     3061 gggggcgacg agcagcaaac catagacggt cagggatagg ggcaggtatc tcgacccagc
     3121 gagcaatacg accacggcga agacgaagaa ggccaggttg aagaagttgc tcgccccggc
     3181 gagttgattc gccacattgc ttagagtggg atgggcccaa aggtcatgac cgaggagcct
     3241 gcccgctcct tcgacggccg atgcccaggc tctgcttgcg gtgtcgagcg gccctgtggc
     3301 ctgcctcccc cagctctcct gcgcggagta gaagagcaga gggtccccga aacgtaccca
     3361 taaataaccc atgtacgcaa atagcccgcc aggcgccagc aacaggtaga tccctcgcca
     3421 acgaaaacgc tctacatcct ttatccactc atacattaga ggtacgacca gaaacacccc
     3481 cacattgcgc gtggcagcag caagagcagc cagaacacac gcaaccaaga gatccttcct
     3541 taccctgatc gcccacaaac tgccggcaga gagcgcaaga aagaggctct ccgtgtacgc
     3601 cgcgttcaag aagaacgtag tagggaagaa ggcgaggata aggacggtgc ccttcgcgac
     3661 cctctcatca aaggcatcaa gggctatgta gtagatgaag tagagggcaa aaggcagaaa
     3721 gaccagggag aggagaggag ccaacacgga gagggcttcc ttggtgatag gaccgccgag
     3781 gagctctgcg aaggatctga gaagcagcgg gtacagcggg aagaaagccg gggagacgtt
     3841 gtcagggggg tggaggtagc cgcccatggc cagtgtgacg tagtgctcac cgtcccagtg
     3901 cgaccagatg ttcatgctcc ctgaaggcac atccagggtt acgctctgga agctagaggt
     3961 cggcaccagg taggcgagca gggagccgga gatcaggtaa aacaggcggc tcgcggcgta
     4021 gaccgcaagc acgaaggcca cacctgtact ctctctgctg tctttctcgt actccatcac
     4081 tggcactagg tctcttgcga agccgcggag gggattatag tcgcgtcagt gccgctcgac
     4141 gatcggtacg tccctgttct ggaaggcggt ccattacagg ttccgtcggg tgatccggcc
     4201 tcgacaattg ttaattctgg cggggcgcga agatgatgac gctgtcgttc tcgtagaccg
     4261 tcctgtagag gtccctctgc agcgcgaagg gacgccagtc tatccaggga tagcgcttgt
     4321 ggaacactat gtagtgcacg tcgtttttct ccaggatctg tgcggtacgc ccgcccgccg
     4381 ggtgctccag cacccacagc gcgtcccaca gcggaggggc cccgaagggc gggaggtccc
     4441 tcgcccgcct tatccttttc ttatcgtagg actgcattcg cgtgtacccg cccatcgcga
     4501 gcatggcccg gctcgggacg tgcttcaggt atggcgtcgc gactatgctt cctccgtcgt
     4561 tgtgctgctt gagccacacc cctgcggcga cgacctcggc cggaggcggc ctgtctttgg
     4621 gtcgcgtgct gggtcccgcg gcttgctcca ggctcagtac ggattgagca cccacgaggg
     4681 taactgaaaa ggtaacggcg agcagggcag ccatatagac ggtacgtccg cccgaccggg
     4741 gcgacagccg caccagggtg acgagggtga gggctgcgag cagtgccagg ggaacgccaa
     4801 ggtcacgctc gaagcgatcg gggaagccgc tgtaggaggt caggctgccg aagaacagca
     4861 gcagggtcca caccagcagg gtgaggcgcg caaggatgcc aggcgtatcc ctggcgttct
     4921 tgcctcctgc gagcatgagc gacaggccga gcaggccgag ccagagaaca gggtgtgaca
     4981 tcgtcgttag gaagtgtcca ggaccgttcg gcggcttggt tccgatcgcc atcgccacgg
     5041 cttcccctcc cctccccgtc tccgacccgc caagcatccc tgccaccagg ctgggcaagt
     5101 cgtaggtgtc ccaagcgtag atcacggaga agagtccgag gagcgcgagc gaaagagcga
     5161 ggtatacccc tttcctgcgg tccctaagga gcaggtaagg caggaagaga acacatacca
     5221 agccgagcag cacggcgagg ctgtagcccg ctatctggtg gtagaggacc gtagaggagc
     5281 cgagcagcgc gagcaagaga cctgcgcgcg ccgacggaga agcgtacatc ccgaccaggg
     5341 cggcgacagc tagcacgatc aggaaatact cccctatgaa gttggggtag cgggcttcct
     5401 cgaagtgcag gtacgtgccg ccgaggagca gacccgaggt gagggccgcg gccaccccat
     5461 actcccagcc ccagaggcgc ttcgcgagcg cgtaacacgc cagggcaccc atcaaaggga
     5521 gcataggagc aaggatcggg aagacctcca gcggctcgat gccgctaagg cgcgagatca
     5581 tggcggtaag gaggtggaag cctggtgggt aaagcatgaa cgactcggca gacccctccc
     5641 tcatcatcat gcctgccatc accgcgtgct cgtagcggtc cacgcccctg ggataggacc
     5701 agccgtttac gatagggccg aggtagccgc gcgcaagcac gagcaggatc accagggaga
     5761 gcaacgcgta gcgccccgcc gcggggaagg atctcccagg ttctgctggg gacgcatctc
     5821 tcccctgcaa tgtcgcccag taggcgatcc cggcagcgag tacaaggacc gctatcggga
     5881 tcatcaacca ctcgccgggc gcaataccga ggaacatcaa gagggccagc gcgagggctg
     5941 cggcgagggg cgc
//
LOCUS       DASTKM010000279         7141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_632291, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000279 DASTKM010000000
VERSION     DASTKM010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632291"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFQ10_11255"
     CDS             complement(<1..603)
                     /locus_tag="VFQ10_11255"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET9965962.1"
                     /translation="MTDSEILSRPRFELFPTKGAEEQADHLPKDAKVAVTCSPTKGIE
                     STLLFSERLLQRGFRVVPHIAARLVADRAHLEEILRWLDEHGLREIHVIGGDSREAVG
                     PYASAFQLLGAMSRLEHGIEEVGIGGYPEGHPLIDDEELDRALLDKQPFASYVVTQLC
                     FDADAILGWISDIRHRGIGLPVYVGLPGVVDRKKLLQVSLK"
     gene            complement(623..1834)
                     /locus_tag="VFQ10_11260"
     CDS             complement(623..1834)
                     /locus_tag="VFQ10_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET9965963.1"
                     /translation="MRIPYDTPRIHMYTRIRKGPFFYAHQRHGVQSYTVTNRQYHPRH
                     YDDILVEYWKLVNDVTMWDVAAERQVEITGPDAFQFTNMLTPRDLNKCSVKQCKFALN
                     TDVDGGIINNPVLLRLGENHFWLSVADGDVLLWAKGVAAVSGLDVQIREPDVAPVQIQ
                     GPRSRDLMVDIFGEEILDLKYYWMDEHDLEGMDVVVSRTGYTGEVGFEIYLRNASRDG
                     MKFWDMMLQAGEPHNLSPIGPSQIRRVEAGILSYGSDIALDNNGYSDYHFLNPYEADL
                     GYTVELDQETDFIGKEALRRIADEGVSRKVVGIELDCDPLIGYIEDYLQVVDDGSQIG
                     QVSSAFWSPRLEKNIGFALVPIEHADLGTGLTVRMETGEYEATVIEKPFIDPRKETVK
                     ADLKVGTARSY"
     gene            2046..2960
                     /locus_tag="VFQ10_11265"
     CDS             2046..2960
                     /locus_tag="VFQ10_11265"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9965964.1"
                     /translation="MDKGLQESPVRVMSFNVRGSFHDVRKRNAWRNRAGLNIATIERY
                     APDVIGLQEAQRGNLGAYRKHLPHYAHIRGPRYGNAIPHDFNTILFDPERLEPLDSGG
                     FWLSETPEKYSRSWETRVARSATWVLFRMIGTELSFLHLNTHLDHVSALARREGSKLI
                     VSRVVEISVRTGVDPPAIVTGDFNSRPGNRTHKTFTESGFADTYLAAGNEEGANTFHA
                     FRGAGYRDAHPHRGPRRIDWIFLKDAQGRLRVESHRIIHDADESSGLYPSDHYPILAD
                     FSWSAGQWAANKETEPQPHESGELTPKS"
     gene            complement(3051..3926)
                     /locus_tag="VFQ10_11270"
     CDS             complement(3051..3926)
                     /locus_tag="VFQ10_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HET9965965.1"
                     /translation="MDTIGITNGRRSERPAETPDLIPGYFARIDKGNLLTHREEIDLR
                     KRAKAGDDRARKKLVEKNLRLVVSVAKRYRGMGLPFEDLIQEGNVGLMRAVEKFDPDR
                     GWRFSTYATWWIRQAVQRAVADKGRTIRVPVHMGDKIRKMARAYNELSTELSREPTDE
                     EVAGRLEWTVGEVLDVKSAMPDATSLNQTLSSDGDGSELGELIEDERASEVAGTVIGE
                     MEVGSLKEAIQRLPERHRHVLVRRYGIGDRDTATLAKLSEELGISRERVRQLQREAER
                     MLGSGELGMVLRGAA"
     gene            4428..4694
                     /locus_tag="VFQ10_11275"
     CDS             4428..4694
                     /locus_tag="VFQ10_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965966.1"
                     /translation="MRSGCLSLVVRVGVVSVAAVLAALSTVLILSFAVSALGQPDGVE
                     KIIKVILATAAGFGFFLVGRGIWRDLRRRSSAEPEEGPTEERGP"
     gene            4762..6291
                     /gene="asnB"
                     /locus_tag="VFQ10_11280"
     CDS             4762..6291
                     /gene="asnB"
                     /locus_tag="VFQ10_11280"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF006949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HET9965967.1"
                     /translation="MCGIIGGYGGMDSESASRMLARVVHRGPDDEGSIEVAGNVLGHR
                     RLSIVDVEGGKQPLATPDGNLYLVGNGEVYNHEEIRETLEDGDMGTRSDNEVALRLIS
                     ERGPGALSELLGMFAFLIAGEDHTFVAARDPVGIKPLYWASRDGLVRFASEMRSFDED
                     WQPDVESFPPGHYWTPQSGLVEFATPVPPRDELEAFDGPGEPGAKIPDEILEKVRGRL
                     IRTVGRQMMGDVPVGVFLSGGLDSSLVAAIAARWYEERGEKLKTFAVGLADSPDLLAA
                     RAVAEYLDTEHHESVYTAEEALGVLPRVVRTIENFDPSLVRSAVPNYILAEFTARHVK
                     VVLTGEGADEIFAGYEYLRDFRTEEELHAELVRSIEGLHDLNLQRADRVTMAHGLEAR
                     VPFLELDMISLGLSLPAGWKLAGEGQEEKRLLRQAFDGWLPDDFLWRKKAQFGDGSGA
                     ASVLKTRMEESVTEEEFVREQHEVEPPLRTREEVAYYRIFAEDLGEVSPKRTIGRFAT
                     T"
     gene            6555..>7141
                     /locus_tag="VFQ10_11285"
     CDS             6555..>7141
                     /locus_tag="VFQ10_11285"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HET9965968.1"
                     /translation="MEKFIISAAITGGMTVPGQSEAIPITPDEIIDSAVGAHEAGATI
                     VHLHVREPETGKPSSDIGLFREVVSGIKDRCDVIVQPTTGGGKGMTLEERAAVLPELE
                     PEMATFNTGSFNFGLFPIAERGGEFEEWEIDYLEGTRDYIFRNTFADMERVCTMFREA
                     GAKPELEAYDVGHLYNIKYLLGRDLLDTPVHIQFVL"
BASE COUNT         1328 a         2232 c         2228 g         1353 t
ORIGIN      
        1 cttcagcgag acctgcagca gcttcttccg gtccacgacg ccgggcagcc cgacgtagac
       61 cggcagcccg atccccctgt ggcggatgtc ggagatccag ccgagaatcg catcagcgtc
      121 gaagcagagc tgggtgacca cataggaggc gaacggctgc ttgtccagca gcgcacggtc
      181 gagctcttcg tcgtcgatca gggggtgccc ctcaggatac ccgccgatac cgacctcctc
      241 gattccgtgc tccaatcgcg acatcgcgcc gagcagttgg aaggcgctcg cgtaagggcc
      301 gacggcctcc ctagagtcgc ccccaatgac gtggatctcc cgcaggccat gctcatcgag
      361 ccaccgcaat atctcttcca ggtgcgccct gtcggccacg agccgcgccg cgatgtgcgg
      421 cacgacccga aagccgcgct gcagcagcct ctcactgaac agcagggtac tctcgatgcc
      481 ttttgtcggc gagcaggtca cggccacctt cgcatctttg gggagatggt cggcctgctc
      541 ctcagcccct ttcgtcggga agagctcgaa cctgggccgc gacagtatct cggagtcagt
      601 catcggcaag cgccccgagc cgctagtacg acctcgcggt ccccactttc aggtccgcct
      661 tgacagtctc ctttctcggg tctatgaacg gcttctcgat gacggtagcc tcgtactcgc
      721 cggtctccat cctcacggtc agccccgttc ccagatcggc gtgctcgatg ggcaccaggg
      781 cgaacccgat gttcttctcc agtcgcgggg accagaatgc cgaggagacc tggcctatct
      841 gcgagccgtc gtcgacgacc tgcagataat cctctatgta acctatcagg gggtcgcaat
      901 cgagctctat gccgaccacc ttgcggctca cgccctcgtc cgcgatgcgc ctgagcgcct
      961 ccttgccgat aaagtcggtt tcctgatcca actccaccgt gtaccccagg tccgcctcgt
     1021 aagggttcag gaagtggtag tcactgtagc cgttgttatc cagcgcgatg tcggacccgt
     1081 agctcaagat gccggcctcg acccgcctga tctgggaagg tccgatcggc gagaggttgt
     1141 ggggctcgcc ggcctggagc atcatatccc agaatttcat gccgtcgcgg ctggcgttcc
     1201 tgaggtatat ctcgaacccc acctcgccgg tgtagccggt ccgcgagacc acgacgtcca
     1261 tgccttcgag gtcgtgttcg tccatccagt agtatttgag gtcgaggatc tcctcgccga
     1321 agatgtccac catgaggtcc ctggacctgg ggccctggat ctgtaccggg gcgacgtcgg
     1381 gttcacggat ctgcacgtcc agaccggaga cggcggccac gcccttagcc cagagcagca
     1441 cgtcgccgtc ggccaccgag agccagaagt ggttctcgcc aagacgcaag agcaccgggt
     1501 tgttgatgat gccgccgtcg acgtcggtgt tgagggcgaa tttgcactgc ttgaccgagc
     1561 acttgttcag gtcgcgcggg gttagcatgt tggtgaactg gaaggcgtcg ggaccggtga
     1621 tctcgacctg ccgctcggct gccacgtccc acatggtgac gtcgttgacg agcttccaat
     1681 actccacgag tatgtcgtcg tagtgcctgg ggtgatactg gcggttggtg accgtgtagg
     1741 actggacgcc gtggcgctgg tgggcgtaga agaatggccc cttgcggatc cgcgtgtaca
     1801 tgtggatcct cggggtgtcg tagggtatgc gcaacagaat acctcctggg atgccgtgat
     1861 ctagtgatcc tcttccttcg aatcagagtg cgtcgctgcg aaaacccctg tattggcctc
     1921 cttgcgtttc tattcgccag ccggaatgta tcatgggcta aattcctgcg caaccatggt
     1981 tgctacaagt gcaaccgctc tgataacgca tgggggtaaa atcgcgagca tgacctggag
     2041 agtacatgga taagggtctg caggagtctc cggtcagggt aatgagcttc aacgtcaggg
     2101 ggtcgttcca cgacgtgcgt aagaggaacg cctggcgcaa ccgggctggg ctcaacatcg
     2161 ctaccataga gcgttacgcg ccggatgtga tcgggcttca ggaggcccag cggggcaacc
     2221 tcggcgcgta ccgcaagcac ctgccacact acgcccacat ccgcggtccg agatacggca
     2281 acgccatccc ccatgacttc aacacgatcc tcttcgaccc cgaacggctg gagccgctgg
     2341 attcaggagg attctggctt tcggagacgc cggaaaagta ctcgaggagc tgggagacga
     2401 gggtggcgcg ctcggccact tgggtgttgt tcagaatgat cgggacagag ctctctttcc
     2461 tccacctgaa cacgcacctc gaccacgtaa gcgccctggc gcgcagggaa ggcagcaagc
     2521 ttatcgtgag tagagtcgtg gagatctctg tccgcacggg cgtcgatccg ccagccatcg
     2581 tcacgggaga cttcaacagc aggccgggaa acaggaccca caagaccttc accgaatcgg
     2641 gcttcgccga cacctacctc gccgccggca acgaggaagg cgcgaatacc ttccacgcgt
     2701 tcaggggagc gggctaccgt gatgcgcacc cccaccgcgg gccgcggcgt atcgattgga
     2761 tcttcctgaa agacgctcaa gggcgactac gggtagaatc gcacaggatc atccacgacg
     2821 ccgacgagag ctcaggactc tatccgagcg accattatcc tatcctggcc gatttcagct
     2881 ggtcagctgg tcaatgggcg gctaataaag agaccgaacc gcagcctcat gaaagcggcg
     2941 aactgacacc aaaaagctga attgctgaca agcggaggcc gccaggccga agcgtgctga
     3001 ccagccgaaa tgcgcgattt gtgggcggct tgggccgccc acaaatcgct ctacgcggcg
     3061 ccgcggagga ccataccgag ctctccgcta ccgagcatac gctcggcctc gcgctgcagc
     3121 tggcggacgc gctcgcggga gatcccgagc tcctcgctga gcttggcgag ggtcgcggtg
     3181 tcgcggtcgc cgatgccgta gcgccgcacc aggacgtgcc tgtgacgctc ggggagccgc
     3241 tggatggcct ccttgaggga gcctacttcc atctccccga taaccgtgcc tgccacctca
     3301 gaggcacgct cgtcctcgat cagctctccg agttcggagc cgtcgccgtc tgaggagagg
     3361 gtctggttga ggctggtggc gtcaggcatc gcgctcttga cgtcgagcac ctcgccgacg
     3421 gtccactcca ggcgtccggc gacctcttcg tctgtgggct cgcggctcag ctcggtggag
     3481 agctcgttgt aggcgcgggc catcttccgg atcttatccc ccatgtgcac ggggacgcgg
     3541 atggtacggc ccttatcagc caccgcacgc tgcacggcct gacggatcca ccacgtggcg
     3601 tacgtggaga acctccagcc gcggtctggg tcgaacttct ccacggcccg catcaggccg
     3661 acgttgccct cctggatcag gtcctcgaat ggcagcccca tcccacgata cctcttggca
     3721 acgctgacca ccagcctgag gttcttctcg accagcttct tgcgcgccct gtcgtcgccg
     3781 gccttggccc tcttgcggag atctatctcc tccctgtgcg tcagcagatt gcccttgtcg
     3841 atgcgcgcga agtacccggg gatgagatca ggagtctcag caggccgctc gctccgccgc
     3901 ccgttggtta tcccaatggt gtccatctaa atcctccttg ctcctataaa accgtctacg
     3961 tcctcaaaat cccgcactac gctcccactt ccggacgcca cgaacccagg gaaaagtacc
     4021 gcgcgagcgc cgcacttcgt tactcgaagt tgtatggatg cgggaatgca ggcttttttt
     4081 aggatcttgc caaactagta gtttacacat ttcggagcgt ttgtcaagag agctcccttc
     4141 ggtcgctcag gctaaaggtg tcaacttgat ggttatcgcg atccgcccca ccagcagccg
     4201 tcacatgctc cggccttgga cctgcgtttg ggagtatagt atctggcgat gaacgaaacc
     4261 gtagagagtc gggtactgat agcgccgtgc ataaagctct cgagccttac gtacgccggg
     4321 ccatcagcac ttcagcaaaa gaagctgact tgctgaagtg ctgaaatgct ggccagcgcg
     4381 gggtggggaa ggtttgacct tccccacccc gctatgagag gagcatgttg cgttcagggt
     4441 gcttgagcct ggtggtaagg gttggcgtgg tgtcggtggc cgcggtcctc gccgcgttga
     4501 gcacggtcct tatcctctcg ttcgccgtct cggcgcttgg gcagccagat ggggtcgaga
     4561 agatcatcaa ggtgatcttg gccacggctg cggggttcgg cttcttcctg gttggccgcg
     4621 gcatctggcg tgacctgagg cgacgttcgt ccgcagaacc cgaggagggg ccgaccgaag
     4681 agcgcgggcc ctagcgggta gggagataag catcccctcc ggggcgacat ctattctgcg
     4741 cccggagtag aacggagtga catgtgcgga atcatcggcg gatatggggg catggactct
     4801 gagtccgcct cgcggatgct ggctcgcgtc gtccacaggg ggcctgacga cgaagggagc
     4861 atcgaggtgg cggggaacgt gctcggccac aggcggctct ccatcgtcga cgtagagggt
     4921 ggcaagcagc cgctcgctac gccagacggt aacctctacc tggtcggcaa cggcgaggtg
     4981 tacaaccacg aagagatcag ggagaccctc gaagacggcg acatgggcac ccgctcggac
     5041 aacgaggtgg cactgcgtct gatctccgaa cgtggacccg gcgccctctc ggaactgcta
     5101 gggatgttcg cgttcctgat cgcaggagag gaccacacgt ttgtcgcagc ccgcgacccc
     5161 gtcggcataa agcctctgta ctgggccagc cgcgacgggc tcgtccgctt cgcctcggag
     5221 atgagatcct tcgacgaaga ctggcagccc gatgtggagt ccttcccgcc gggccactac
     5281 tggacccccc agagcggcct cgtggagttc gccacacccg tcccgcccag agacgagttg
     5341 gaggccttcg acgggcctgg agagcctggc gcaaagattc cggatgagat actggagaag
     5401 gtccgcggcc gcctaataag gaccgtcggg cgccagatga tgggcgacgt gcccgttggc
     5461 gtcttcctct ccggcggcct cgactccagc ctcgtcgccg ccatcgcagc ccgctggtac
     5521 gaggagcgcg gcgagaagct caagaccttc gccgtcggcc tcgcggactc gccggacctg
     5581 ctggccgccc gtgccgtcgc cgagtacctc gacaccgagc accacgagag cgtctacacg
     5641 gcggaggagg cgctcggggt gttgcccaga gtagtcagaa ccatcgagaa cttcgatcct
     5701 tcgctggtac gtagcgccgt tcccaattac atcctggctg agttcacagc aaggcacgtc
     5761 aaggtcgtgc tgacaggcga gggggccgat gagatcttcg ccggctacga gtacctcagg
     5821 gatttccgca cggaggaaga gttacacgcc gagctcgtgc ggtctatcga ggggttgcac
     5881 gacctcaatc tgcagagggc cgaccgcgtt acgatggccc acggcctcga agcgcgggta
     5941 ccgttcctgg agctggacat gatctccctc ggactctccc tcccggccgg gtggaagctc
     6001 gccggcgagg gccaggaaga aaagaggctt ctccgccagg ccttcgacgg ctggcttccc
     6061 gacgattttc tgtggcgcaa gaaggcccag ttcggggacg gctcgggcgc ggcctcggtc
     6121 ctcaagacgc gtatggagga gagcgtcacc gaggaagagt tcgtgcgcga gcagcacgag
     6181 gtcgagcctc ccttgcgtac ccgcgaggag gtcgcctact accgcatctt cgccgaagat
     6241 ctcggcgagg tcagcccgaa gcggactatc ggacgcttcg caaccacgtg aatcagcatt
     6301 tcagcacgct ccagccttcg gctgtcgctt gtcagcacgc cgttctgcgg gcggcttgtc
     6361 agcatttcag cttcttgctc tcgctaccga tccgggatgc cagcgcatca ctcatttgca
     6421 gtatttgttg ctcggaggtt cgctctttcc cagaatagta ctgtgatttt tgcacgaacc
     6481 cgtgcccggt ccggttagcg gcggtcctgg tgggtatcta gtattgggtc aggactctaa
     6541 cgaagggagc gggcgtggag aagttcatta tcagcgcagc gattacgggc gggatgaccg
     6601 tgccagggca gagtgaggcg atccctataa cgccggatga gataatcgac tccgccgtcg
     6661 gggcgcacga ggccggggcg actatcgtcc acctccacgt ccgtgagcca gagacgggga
     6721 aaccgagctc ggatataggg cttttccgcg aggtggtctc ggggatcaaa gaccgctgcg
     6781 acgtgatcgt acagcccacc acgggcggcg gtaaggggat gacgctggag gaaagggcag
     6841 ccgtcctgcc ggagctggag cccgagatgg ctactttcaa caccggcagc ttcaacttcg
     6901 gcctcttccc gatcgcagaa cggggcggag agttcgagga gtgggagata gactacctgg
     6961 aaggcacacg cgactacatc ttccgcaaca ccttcgcgga catggagagg gtctgcacca
     7021 tgttccgcga agccggcgcg aagccggagc tagaagccta tgacgtcggg cacctctaca
     7081 acataaaata cctgctcggc agggacctgc tcgacacccc ggtccacatc cagttcgtcc
     7141 t
//
LOCUS       DASTKM010000280         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1163822, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000280 DASTKM010000000
VERSION     DASTKM010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163822"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFQ10_11290"
     CDS             <1..255
                     /locus_tag="VFQ10_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET9965969.1"
                     /translation="VKRPDAVMVFVRAPSLEETRRRLTGRATESEEALESRLATAVGE
                     VAARDEFDHEVINENRERARKDMIEKMESIVRGGKDAARP"
     gene            239..601
                     /gene="rpoZ"
                     /locus_tag="VFQ10_11295"
     CDS             239..601
                     /gene="rpoZ"
                     /locus_tag="VFQ10_11295"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET9965970.1"
                     /translation="MLHDLKIDELLDKVDSRYGLAVASAKRARQINEYTSTLGLNDAL
                     GIPGPQVHTRSQHPLTIAIEELRQDKLDVSFRQPSDNMDEDIGAADTDDFISPPAGPG
                     VPQQTDADSGAPGDEGAA"
     gene            561..1688
                     /gene="coaBC"
                     /locus_tag="VFQ10_11300"
     CDS             561..1688
                     /gene="coaBC"
                     /locus_tag="VFQ10_11300"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET9965971.1"
                     /translation="MPTRAPLATKALPEISLNTLILLSVGASQGALTMPAVASEIAET
                     GNSVEVILGQRARRFVGPAAFREVALVDDPSETPEALLFAPAPVDTLARLARGMDTRF
                     SASTVVAPDLDAGTAGHPAVRENLALLREDGRRIIEGQDGGMASTGEIVAGLLGSLRG
                     PMSGLRLLVTAGGTREPIDSVRFIGNRSSGKMGLAVAREALRLGAGVTVVAANVDRVE
                     PGTRYHTVETVEELREAVLDRAADAHALVMAAAVSDFTPASRVRTKIRRSTGTTSLEL
                     APTADILEAVREQYPDLFVVGFAATHGDPVADAREKLTRKGANLVVGNDISREGVGFG
                     AAENEVYIVGRKKERFVPRAPKDEVARVILDSLMAEIDEER"
     gene            1694..2926
                     /gene="metK"
                     /locus_tag="VFQ10_11305"
     CDS             1694..2926
                     /gene="metK"
                     /locus_tag="VFQ10_11305"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET9965972.1"
                     /translation="MERAAGPTTWNAADDQKLKGTHLFTSESVTEGHPDKIADQISDT
                     ILDAAIADDPMSRVACETLVTTGLVVVAGEITTSTYVDIPTLVREKVSKIGYTRAKYG
                     FDAETCGVIVSIDPQSADIAQGVDRALEAREGEGLQEIGAGDQGMMFGYACEETDELM
                     PLPITLAHALTRRLSDVRKSRALDYLRPDGKSQVTVRYEGDRPVGIEKILISAQHRDG
                     VEAHIKDDLLETVIRPVMPEELFDSGVAEIMVNPTGRFVTGGPQGDTGLTGRKIIVDT
                     YGGSAPHGGGAFSGKDPTKVDRSASYAARWVAKNLVAAGLAGRCLVQVAYAIGVAHPV
                     SVMVETFGTARIDPATLGALVQEHFDLRPGAIIRDLKLRNPIFARTAAYGHFGRREPG
                     FTWEETSLSDDLRRAAGF"
     gene            2939..>3785
                     /locus_tag="VFQ10_11310"
     CDS             2939..>3785
                     /locus_tag="VFQ10_11310"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965973.1"
                     /translation="MDRATSRATRKPHAARVSLLVPTHALPPLSYRIPERLGARIRVG
                     TAVVAPLSGRQRLGIVVGTEEQSDRAREAVVSVVPDLALQPELVRLCHWIHANAAVPL
                     PAVLRAALPPGIEAGRYRVIEPSPGWPWDVDSSVTRATLERTLGPDGLRAAQAEGRVV
                     LEPSAPQPRTVEWTVIRAAADPDLTRAPRQRDLFVTLKERGGSSITSVLLSETGASRN
                     SLRELVRRGAAKLVRRQKPGPLLATFGDPGDSEDLVPFSRTARRAVRSGGPFLWRTTG
                     REEHAA"
BASE COUNT          732 a         1203 c         1260 g          590 t
ORIGIN      
        1 gtaaaacgtc ctgatgccgt tatggtcttc gtgcgcgctc cttcgctgga ggagacccgc
       61 cgcaggctca caggaagggc tactgagtcg gaagaagccc tggagagcag gctggcgacg
      121 gccgtggggg aggtcgcggc cagagacgag ttcgaccatg aggtcataaa cgagaaccgg
      181 gagcgggccc gcaaggacat gatcgagaag atggagtcga tagtaagagg aggcaaagat
      241 gctgcacgac cttaagatag acgagcttct ggacaaggtg gactcccgct acggcctcgc
      301 cgtggccagc gccaagagag cgcgccagat caacgagtac acctcgaccc tgggcctcaa
      361 cgatgccctc ggcatccctg gccctcaggt ccacacccga tcccagcatc cgctgaccat
      421 cgccatcgag gagttgcgcc aggacaagct ggacgtctcc ttcaggcagc cttcggataa
      481 catggacgaa gatatcgggg cggctgatac cgatgacttc atcagcccgc ccgccggccc
      541 tggcgtcccc cagcagaccg atgccgactc gggcgcccct ggcgacgaag gcgctgcctg
      601 agataagcct taacaccttg atccttctct ctgtcggagc ttctcagggg gcactgacga
      661 tgcccgctgt agcaagcgag atagctgaga caggcaacag tgtggaggtg atcctaggcc
      721 agagagcccg tcgcttcgtc ggccccgcgg cgttccgtga ggtagccctg gtagacgatc
      781 cctcagaaac acctgaggct ttgctcttcg ctcctgcacc cgtggatact ctggccaggc
      841 tcgcccgcgg catggacacc cgcttctcgg cgagcactgt cgtggccccg gatctcgacg
      901 ccgggacggc cggccacccc gccgtgcgag agaacctcgc tctcctccga gaagacggtc
      961 gccgcatcat cgaggggcag gatggcggga tggcctccac gggagagatc gtcgctgggc
     1021 tactcggaag tcttcgcggc ccgatgagcg ggctgcggct gctcgtgacc gctggcggca
     1081 ccagagagcc catagacagc gtgcgtttta tcgggaatcg ctcctcgggg aagatggggc
     1141 tggcagtggc ccgcgaagct ttgcggctcg gcgccggggt taccgtagtg gccgccaacg
     1201 tagatagagt cgagccgggg accaggtacc atacggtgga gaccgtggag gagctgcgag
     1261 aggctgtact cgaccgtgcc gccgacgcgc acgcgctcgt catggccgca gcggtttcgg
     1321 acttcacgcc ggcctcgcgg gtaaggacga agatccggcg cagcacaggg acgacgagcc
     1381 tggagcttgc ccccacagcc gacatactcg aggccgtcag ggaacagtat cccgacctct
     1441 ttgtggtagg attcgcggcg acgcacgggg accccgtggc cgacgcccgc gagaagctca
     1501 cacgaaaggg agcgaacctc gtggtgggca atgatatctc cagagaaggc gtaggatttg
     1561 gcgccgccga gaacgaggtc tacatcgtcg gccgcaagaa ggagcgtttc gtccccaggg
     1621 cccctaaaga tgaggtcgcc cgtgtcatct tggacagcct gatggcggag attgacgagg
     1681 agagatagag atcgtggaga gagcggcggg cccgaccacc tggaacgcgg cggacgacca
     1741 gaaactaaag ggaacccacc tctttacctc agagtccgtc accgagggcc accccgacaa
     1801 gatagcggac cagatctccg acaccattct ggacgccgca atcgcggacg accccatgag
     1861 cagggtggcc tgtgagacgc tggttaccac aggactcgtc gtcgtggccg gtgagataac
     1921 gacctcgacc tacgtggata tcccaaccct ggtgcgtgag aaggtctcga agatcggcta
     1981 caccagggcc aagtacggct tcgacgccga gacctgtggc gtgatcgtct ccatagaccc
     2041 gcagtccgca gatatcgccc agggggtgga cagggcgctc gaagcaagag agggcgaggg
     2101 tcttcaggag ataggggctg gcgaccaggg gatgatgttc ggctacgcct gcgaggagac
     2161 cgatgagttg atgccgctcc cgatcacact ggcccatgcc ctgacgcgcc gtctctccga
     2221 cgtccgcaag agccgcgccc tggactacct caggccagac ggcaagagcc aggttaccgt
     2281 ccgctacgag ggggacaggc ccgtaggcat cgagaagata ctgatctcgg cccagcatcg
     2341 cgacggggtg gaggcccaca taaaggacga tctcctggag acggtcatac gccccgtaat
     2401 gcctgaggaa ctcttcgact ccggcgtggc cgagatcatg gtcaacccca ctggcaggtt
     2461 cgttacgggc ggaccacagg gcgacacggg cctcacgggg cgcaagatca tcgtggatac
     2521 ttacgggggt tcggcccctc acggcggtgg cgctttctca ggcaaggacc cgaccaaggt
     2581 agaccgctcg gcctcctacg ccgcaaggtg ggtggcgaag aacctcgtgg cggcaggcct
     2641 cgcaggccgg tgcctggtcc aggtggccta cgcgatcggt gtggcccatc ccgtgagcgt
     2701 gatggtcgag acgttcggca ccgcaaggat tgacccggcg accctcggcg cgctggtgca
     2761 ggaacatttc gacctcaggc ctggcgcaat catccgcgac ctgaagctgc ggaaccctat
     2821 ctttgcgcgg accgcagcat acggccactt cgggcgcaga gagccaggct tcacctggga
     2881 ggagacctcg ctgtcagatg atcttcgccg cgccgccggc ttctaattgg taaagagggt
     2941 ggaccgggcg acgtcccgcg ccacgagaaa gccgcacgcg gcacgagtaa gcctgctcgt
     3001 acccacgcac gccctacctc cccttagcta caggatacct gagcgccttg gggcaaggat
     3061 tcgggtagga acagcagtcg tcgcgccgct ctcgggaagg cagcgcctcg gcatagtcgt
     3121 tggtaccgaa gagcagagtg accgcgcccg agaagcagta gtctccgttg tgcccgatct
     3181 cgcgctgcaa cccgagctcg tacgactatg ccactggatc cacgctaacg cagcggtccc
     3241 tttgccggcg gtgttgaggg ccgccctgcc gccgggcatc gaagcgggac gctaccgcgt
     3301 gatagagcct tctcccggat ggccttggga cgtcgacagt agcgtcacgc gcgcgaccct
     3361 cgagcgcacc ctcgggccag atggcctgcg cgcggcccag gcggaggggc gggtcgttct
     3421 cgaaccgtca gcgccgcaac cgaggacggt ggagtggact gtgatccgtg ccgccgcaga
     3481 ccctgacctg acccgtgccc cacgccagcg cgacctgttt gtgaccttga aagagcgcgg
     3541 aggctcgagc attacctcgg tcctgctctc ggagaccggc gcgagccgaa actccctacg
     3601 ggagctcgtg cgcaggggcg cagcaaagct ggtgcgccgc cagaagccag gccccttgct
     3661 ggccaccttc ggcgaccctg gcgactctga agacctcgtc ccgttctcac ggaccgcgcg
     3721 gcgcgccgtg aggagcggcg gccctttctt gtggaggacg accgggcgag aggagcacgc
     3781 cgcgg
//
LOCUS       DASTKM010000281        11131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_284300, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000281 DASTKM010000000
VERSION     DASTKM010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 11131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11131
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284300"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFQ10_11315"
     CDS             <1..482
                     /locus_tag="VFQ10_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HET9965974.1"
                     /translation="KQREYEGYHYEAADASLALLIGRTIGEDRPLFELETFRIISEKR
                     AGGETTTEATLKLSVGGQRVISTAEGNGPVNALDQALRKAIGPHYPELKEIHLSNYKV
                     RILDEHRATAATTRVLIDSTDGKRMWGAVGVSENIIEASWQALVDGLEYGVNGTSKA"
     gene            complement(678..821)
                     /locus_tag="VFQ10_11320"
     CDS             complement(678..821)
                     /locus_tag="VFQ10_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965975.1"
                     /translation="MLEEQETEAQRVRLEIETRGRAALTEAENEASNQPSPPSSSPGS
                     SPS"
     gene            complement(884..1771)
                     /locus_tag="VFQ10_11325"
     CDS             complement(884..1771)
                     /locus_tag="VFQ10_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9965976.1"
                     /translation="MMTESLEARFQTRTVARMQEFSRIKEVVSGWSGVRQYLRGADEG
                     QIVPVVIPKDRRRYGWLFLLGVAIYMLGFALFSGSAALAILAVLVAVIFLGLALLWLW
                     RGAIVEIEQGTTGVRSRYGKIEGTLRHYLWRPWDRVEFVVDTATEIPYLAPVADCPTR
                     ENVPLKAIEFFLKFRIVDPLAFVRVIGASNFDLVLSSAVQDAIRQRSRQVSTEKAYDL
                     RGSDVGDMQEYLNRQLAHYGVRILGANIPDVQLPDQYQQHLATRERVAKELAAYEREW
                     ELTRKQRIDTLLMDIERSK"
     gene            1970..3373
                     /locus_tag="VFQ10_11330"
     CDS             1970..3373
                     /locus_tag="VFQ10_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965977.1"
                     /translation="MLLAQGLAALLFLRHGWDALAFPYPLNYGEGPLLDQSVRLAQFQ
                     NIYRTDLTVSPYVISNYPPLFMLAQVPLVWLFGPALWYGRLISLLSVAAVALFVGLTL
                     HALTKDRIAAIAGGLIFPAMPYVVRWSSLSRVDSLGLALSWAGLFVVARWPEKRWSVF
                     VSALLLVAAVYTRQTYVLAAPLAAFVWLAAQGQRRRALETVAIAGGAGLVVFALLNIF
                     TGGGFFLNTVTANLNDFRWERVSLNALGALLACPLLLLASLAFLVLTPRKGNRSWWLV
                     APYLLASIPSAVLVGKVGSDVNYLLESSAALGLATGAFIAWQRRRPRLRIALIALLAV
                     QVLALAQSSRVPSGLQNYVIEQRGEVTLLSRMVANANGPVLTDDYMGLLPQRGRRIYF
                     QPFEMTQLTLDGDWDQRPILKDIEEEKFPMIMIWSPPFAREIKRDRWTPRMLEEIHDN
                     YERTDRVADMVIYRPRE"
     gene            complement(3406..3531)
                     /locus_tag="VFQ10_11335"
     CDS             complement(3406..3531)
                     /locus_tag="VFQ10_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965978.1"
                     /translation="MSSLAVVIADTHLPRRASALPEGLIPHLEQLMRGEEVAKDA"
     gene            complement(3528..4292)
                     /locus_tag="VFQ10_11340"
     CDS             complement(3528..4292)
                     /locus_tag="VFQ10_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965979.1"
                     /translation="MNEVPGRGIANPIGIEGLGGDLPIFGAVEALFLVAAVGAVASVA
                     VRFRRSRGVERQQMKLFAYVIVVLIGGSVLAGGISDLTGVGWLEGSSFVLSMMGLMCL
                     PIAVGIAILRYRLYNIDLIINRTLVYGPLTATLALVYFGGVATTQLVFRALTGQQQQP
                     QLAIVVSTLVIAALFNPLRRRIQAFIDRRFYCSKYDARKTLEAFSASLRDETDLDTLN
                     NQLVGIVRQTIQPAHVSLWLRHDTPMKVKQEDQPTM"
     gene            complement(4435..4809)
                     /locus_tag="VFQ10_11345"
     CDS             complement(4435..4809)
                     /locus_tag="VFQ10_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965980.1"
                     /translation="MSRRALLLPLARVSGWLIVPLYRAGACAYYFFIISAGFWDGDAV
                     FDNVTGVILGVGFGAFAVVGALLVVRRPTNAIGWILAAIGLMVSIFNAGGGYATYVMV
                     SPGAGPTPSPSSARGPQTATGS"
     gene            complement(4967..6580)
                     /locus_tag="VFQ10_11350"
     CDS             complement(4967..6580)
                     /locus_tag="VFQ10_11350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET9965981.1"
                     /translation="MIEPPENAQHAQGTHRTAVTMAVIAVMISLFPLLFACSSEGSKD
                     HSDGGRRTEEGQRDSATRGGQTGDGSKGASRAETQAAIVPIAHLSSTAENISTQELSG
                     TRNVAVGRGYRDEAEELLDSPRFESFDSGDAVVEHVSKTPEALGLVPWDEVGPRVKAL
                     SVDGRSLLDSEGSGDYPLRPDGATVPDPQELRRVVVGGDIVLDRGQYYTVIQKGMGID
                     FPLDGGYAAVTSRVPEQSAYSETGIIHQFTARRTGRAGAVREYLSGADLTLANFENPV
                     IRDAVYHPDATTFTGHLRLMPVLDQAGMDGVTLGNNHVLDAGTTGLNETMRHLDDAGI
                     AHAGAGMDLAEARKPMVFDLGGTKVGVLSYLDVPSYGWAWATQSAPGTAPLLQNVMEE
                     DIKRLRPRVDLILVMPHWGKEYIATPEPQQVDLAHAALDAGADLVIGGHAHWPKGIEI
                     YKGKPIFYGVGNFLLDQSWSEETSTGIFAEITLYGDDVVQVRPVPFIMLDFAQPNFLM
                     PGAGGDRALRKIFAASLGPEFEAYGSHAR"
     gene            complement(6633..6884)
                     /locus_tag="VFQ10_11355"
     CDS             complement(6633..6884)
                     /locus_tag="VFQ10_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965982.1"
                     /translation="MKRIPRIPDPGQHVDLRTEDGSWRKGFRALSEPWTAETGDVMIW
                     VATEDEYRAAGREGRSAVGVPWPTERRRLSYPHLPWRLS"
     gene            complement(6917..7255)
                     /locus_tag="VFQ10_11360"
     CDS             complement(6917..7255)
                     /locus_tag="VFQ10_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965983.1"
                     /translation="MREAARRTVYDFVVITLTRVPNVIVIMAAVVLSYLVYRRRPKPP
                     KWGMWPAIFAVLALYGFLAALAPAGWDEPLVLLGVLFAALAGYAWLDPARRASSAGAG
                     QTSGGRGWFG"
     gene            complement(7678..9081)
                     /locus_tag="VFQ10_11365"
     CDS             complement(7678..9081)
                     /locus_tag="VFQ10_11365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9965984.1"
                     /translation="MTTLEELRKVPLFEGLTDEQLREILEEGSEEFILAGEVGGREGE
                     PVDHLSVILEGEFRWSKKVDGGEVVMNTYGSGEFFAEVPLLLGKPFLATWRALVNSRV
                     FALPNETFRRMLATYPSFSNTVLEMMAQRIQVLYSVAQQRERLSSLGTLAAGLAHELN
                     NPSSASRRAAGLLRESLENRRSLGIRLTRAAARGEIDPVQLDALERIVHEAFGRDNAP
                     ALSSLERSDLEDEVALWLEDRGVGEAWDLSPTLVDAGLGIAELKLVESAVPPITLTDA
                     LGYLEAVLGTARLVDEIEASSMRISNLVATMEAYSYMDRSPTQEVDVNEGLDNTLAIL
                     RYRLTGIEVERDYDESLPRISAHGSELNQVWTNLIDNAIDAVEDGDKTGRIRLRTTYE
                     RDRLLVEISDNGAGIPEEIQDRIFEPFFTTKEVGKGAGFGLDVSYRIVVGRHGGDIRI
                     VSKPGDTRFQVRLPMEA"
     gene            complement(9387..9581)
                     /locus_tag="VFQ10_11370"
     CDS             complement(9387..9581)
                     /locus_tag="VFQ10_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965985.1"
                     /translation="MKDTYRYYILAYEDARQKRPIKHEYGGTTVLTDGAEALGRAEVL
                     AKEYPDGWVYIHVEYRGDKV"
     gene            complement(9852..>11131)
                     /locus_tag="VFQ10_11375"
     CDS             complement(9852..>11131)
                     /locus_tag="VFQ10_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965986.1"
                     /translation="AVAGALLLFRKRRANKRRIEEERRSAEEEFANLTVHLNEFDEKE
                     RLASGYLEAQRSLLDQRTEEWVESRISDAKTTGFAEEFDEAASRLTSDPSSARERVGH
                     GRNLLAESMQRLHEAERTIDDYRAADEALDGRLRVAKEEIQAAEEAEEIARAEGIAVE
                     PLKLRPEYDRVAREAADRASRRDEFDPRHSLAAVEALAEQARAHSKALQNEISARDAL
                     PDERYATEGAIVRARETLEEYRGAHDRALDEFGQAALGEVPNPGELSSSLLEAEGYVD
                     RADRAASSGRFSEARSLLHEAAELARNAMRSPGRLKAATAEADRKKREGEEKLQELEA
                     RLAQAKANEHLMDPHQRQRLREYEYQLGNARYGFFGGDWLAALLLFEALDNDYMHMGD
                     PSSFDTGGFGSDWGGGDWGGGDWGGGDFGGGDF"
BASE COUNT         1950 a         3631 c         3387 g         2163 t
ORIGIN      
        1 taaagcagcg cgagtacgag ggctaccact acgaggccgc tgacgcctcg ctcgccctgc
       61 tgatagggcg gaccatcggg gaggacaggc cgctcttcga gctggagacg ttcaggatca
      121 tctccgagaa gcgggccggc ggggagacga ccacagaggc taccctgaag ctctccgtag
      181 gcgggcagag ggtgatctct accgcagaag gcaatggtcc cgtcaacgcc ctggatcagg
      241 cgctaaggaa agccataggg ccgcattatc ctgagctgaa agagatccac ctctccaact
      301 acaaggtccg catcttggat gagcaccgcg ccacggcggc cacgacccgc gtcctaatcg
      361 actcgacgga cggcaagcgg atgtggggcg ccgtaggggt ctccgagaac atcatcgaag
      421 cctcctggca ggctttggta gacggtctcg agtacggcgt gaacgggacg agtaaagcgt
      481 gatcctacac cggtcccacg ccacttctcg cactcagcct gggagatact cgagaacacg
      541 ccctgagcga cttccgtcgt ggattcccgc tttcgcgggg acgggcccca ctggaatgac
      601 ggttatgctg tgagactacc cgtcgccaag gtcgcgcaga gcctcacccg ctttctccac
      661 tgtcgagccg tccgcggtca gctgggggag gagccgggcg aggatgaggg tggagagggc
      721 tgattgctcg cctcgttctc cgcctctgtt agggcggcgc gacctcgcgt ctctatctcc
      781 aggcgaacgc gctgggcctc ggtctcctgc tcttcgagca tacgtgcaac gtcttggcgg
      841 gcgcggttgc gggcttcacc cctttcaggt gggcgtcgcg gtcttacttg gaacgttcga
      901 tatccatgag gagcgtatcg atgcgctgct tgcgggttag ctcccactcc cgctcgtagg
      961 cggcgagctc tttggccacg cgctcgcggg tggcgaggtg ctgctggtac tggtcgggga
     1021 gttgcacgtc ggggatgttg gcgccgagga ttcggacccc gtagtgggca agctggcggt
     1081 tcaggtactc ttgcatatct ccgacgtcgc tgccgcgcag gtcgtaggcc ttctccgtgc
     1141 tcacctggcg gctgcgctgc cggatcgcat cctgcacggc gctggagagg acgaggtcga
     1201 agttcgaggc tccgatgacg cgtacgaagg cgagcgggtc gactatcctg aacttcagga
     1261 agaactctat cgccttcagc ggcacgttct cccgcgtggg gcagtcggcc acaggcgcca
     1321 ggtaagggat ctccgtcgcc gtgtcgacga cgaactcgac cctgtcccac ggacgccaga
     1381 ggtagtgcct cagggtgcct tcgatcttcc cgtagcgcga gcgcacaccc gtagtcccct
     1441 gctctatctc gacgatggcg ccccgccaca gccacagcag cgcgaggccg aggaagatga
     1501 ccgcgaccag cacagccagg atggcgagcg ccgcgctccc cgagaagagt gcgaagccga
     1561 gcatgtagat cgccacaccg agcaagaata gccagccgta gcgcctgcgg tccttgggta
     1621 tgacgacggg tacgatctgg ccctcgtccg cgcccctcag gtactgcctt accccagacc
     1681 agcccgagac gacctctttt atcctggaga actcctgcat cctggcgacg gtccgcgtct
     1741 gaaaccgcgc ctccagcgac tcggtcatca tcgctcctcc ccgtcgtcct tagaacggtc
     1801 agtagagagc gttgggcatt gttcgcgcgt agccggcgcc aaaagcggta ggatacagag
     1861 cctgagttcg agaacatgga ggggcctggc gacgagtacg ggtttagaga aagcagccca
     1921 caagggcggg cggacgcgcc gggtcgcgtc atttgccgca atcgcgctgt tgctccttgc
     1981 ccagggtctt gccgcgctgc tctttttgcg gcacggatgg gacgcgctcg ctttccccta
     2041 cccgctcaac tacggtgagg ggccgctgct cgaccagtcc gtaaggctcg cgcagttcca
     2101 gaacatctac aggaccgacc tcaccgtctc gccctacgtt atctccaatt atcctccact
     2161 cttcatgctc gcccaggtac ctctggtatg gctgttcggg cctgccctct ggtacgggcg
     2221 tctgatctcg ctcctcagcg tcgctgctgt ggcgctcttt gtggggctga ccttgcatgc
     2281 gctgaccaaa gacaggatcg cggcgatagc gggaggtctg atcttccctg ccatgcccta
     2341 tgtggtgcgc tggtcatcgc tctccagggt cgactcgctg gggctggcgc tctcctgggc
     2401 cgggctgttc gtcgtcgcgc gctggcccga gaaacgctgg agcgtcttcg tctcggccct
     2461 gctcctcgtg gcggccgtct atacccgcca gacctacgta ctggccgcgc cgctcgcggc
     2521 tttcgtgtgg ctcgcggccc agggccagcg tcgccgggcg ctggagacgg ttgccatagc
     2581 cggcggagca ggccttgtcg tgttcgccct actgaacatc ttcaccggag gcgggttctt
     2641 cctcaacacc gtaaccgcca acctcaacga tttccgttgg gagcgggtta gcctcaacgc
     2701 cctcggggcc ctccttgcct gcccgctgct actgctcgcc tcgctcgcgt tcttagtgct
     2761 tacgccgcgt aaggggaacc gttcctggtg gctcgtcgca ccctatctgc tcgccagcat
     2821 cccctctgcg gtgctcgtcg gcaaggtggg ttctgacgtg aactatctgt tggagtcatc
     2881 ggcggcgctg ggcctggcaa caggggcatt tatcgcctgg cagcgcaggc ggccgcggtt
     2941 gcggatcgcc cttatagccc ttctcgctgt ccaggtactg gccctcgcac aatcgtctcg
     3001 ggtcccttca ggtctgcaga actacgttat cgagcagcgg ggcgaggtca ccctactatc
     3061 ccgtatggtg gcgaatgcga acgggcccgt gctcacagac gactacatgg gactcctacc
     3121 ccagagaggg cggaggatct acttccagcc tttcgagatg acccagctca cccttgacgg
     3181 cgactgggat cagaggccga tcctgaagga catcgaggag gagaagtttc ccatgatcat
     3241 gatctggagc ccacccttcg cgagggagat aaagcgcgac aggtggaccc caaggatgct
     3301 cgaggagata cacgacaact acgagagaac ggatcgtgtg gccgatatgg tgatctaccg
     3361 cccgagggaa tagggggcgg gttcgtgtaa gggcgcttcg cgcccctacg cgtccttcgc
     3421 gacctcctcg ccgcgcatga gttgctccag gtggggtatc aggccctccg gcaatgcgct
     3481 ggcgcgccgc ggtaggtgcg tgtcggcgat gacgacggcg agcgaactca catagtcggc
     3541 tggtcttcct gcttaacctt catgggtgta tcgtgacgca gccagagtga gacatgcgcc
     3601 ggttgtatcg tctgcctgac gatcccgacc agttggttgt tcagcgtgtc caggtccgtt
     3661 tcgtcgcgca ggctggcaga gaacgcttcc agggtctttc tggcgtcgta cttgctgcag
     3721 tagaagcgcc tgtctatgaa ggcttggatg cgtcgcctca atgggttaaa cagtgctgct
     3781 atcacgagcg tggagacgac tatggcgagc tgaggctgct gctgttggcc tgtgagcgca
     3841 cggaacacga gctgggtcgt cgccaccccg ccgaaataca ccagcgccaa cgtcgccgtg
     3901 aggggaccgt agaccagcgt acggttgatg atgaggtcta tgttgtaaag gcggtagcgg
     3961 aggatagcta tgccaacagc gatcggcaga cacatcaggc ccatcatgct gagcacgaaa
     4021 ctgctcccct ccagccatcc cacgccggtc agatcggaga tgccgccggc gaggacggag
     4081 ccgccgatca ataccactat aacgtaggcg aacaacttca tctgctggcg ctccaccccc
     4141 cgcgagcggc ggaagcgcac cgccaccgac gctaccgccc caacggctgc gacgaggaaa
     4201 agagcctcga ccgcgccgaa tatcgggagg tccccgccca gaccttcgat gccaatggga
     4261 ttggcgatcc ctcgaccggg aacttcgttc acgggaaggg tgtccactag tgccctgggc
     4321 aggacgatgc ccagtgtgga gaccccccga tcacggccac cgggagctag cgacgagaga
     4381 ggaaccgccc gtcggggaag agcatgggca ggtagatgag ggccagcgcg agcatcagga
     4441 accagtagca gtttgcggcc cacgcgccga agacggcgag ggcgtcgggc cggccccggg
     4501 ggagaccatc acataggtag cgtagccgcc accggcgttg aagatcgaca ccatgagacc
     4561 gatggcagcc aggatccagc cgatggcgtt ggtgggccgt cttaccacaa gcagcgcccc
     4621 gaccaccgcg aaggcaccga accccacacc caatatcacg cctgtcacgt tgtcgaacac
     4681 tgcgtcgccg tcccaaaagc cggcgctgat tatgaagaag tagtaagcgc aagcgccggc
     4741 tctgtacaag gggacgatca gccaccccga cacgcgcgca agaggtaaga gcaaagcgcg
     4801 acgactcata ccaccgactt cttgcccctg ttctctcgtc ctttcagtca cagcaccact
     4861 ttacatcacg cccgtctccc aaacgttacg acgacagccc tgtagtccgc ggggaagaac
     4921 cttcgaggag acttcgactt ctccctcgcg cctcgttgaa ggaacgtcag cgggcgtggc
     4981 tcccgtacgc ctcgaactcg ggaccgaggg acgcggcgaa gatcttgcgc aacgccctgt
     5041 caccgcccgc ccctggcatg aggaagttgg gctgggcgaa gtcgagcatg atgaagggga
     5101 cggggcggac ctggaccaca tcgtccccgt acaatgtgat ctctgcgaag atccccgtcg
     5161 aagtctcttc cgaccaggat tggtcgagga ggaagttgcc gacaccatag aaaatcggct
     5221 tccccttgta aatttcgatc cccttgggcc agtgggcgtg gccgccgatt acgagatcgg
     5281 caccggcgtc cagggcggcg tgtgcgagat ccacctgctg cggttctggg gtcgcgatgt
     5341 actccttgcc ccaatgtggc atcacgagga tcaggtcgac tctcggacgc aagcgtttta
     5401 tgtcctcctc catgacgttc tgaaggagtg gcgccgtgcc gggggccgac tgggtagccc
     5461 aggcccaacc gtaggagggc acgtccaggt atgagagaac accgaccttc gtcccgccga
     5521 ggtcgaacac catgggcttg cgggcctccg caagatccat acctgcccca gcgtgggcta
     5581 ttccggcgtc atccaggtgt cgcatcgtct cattcagccc tgtagtccct gcatccagga
     5641 cgtggttgtt gcccagggta acgccatcca tgccggcctg atcgagtaca ggcatcaacc
     5701 gcaggtggcc cgtgaaggtc gtcgcgtcgg ggtgatagac ggcgtctctt atgaccgggt
     5761 tctcgaagtt agcaagcgta aggtcggcgc ccgacaggta ctccctgacc gcgcccgctc
     5821 tcccagtgcg cctggccgtg aactggtgga tgatccccgt ctccgagtag gcgctctgtt
     5881 ccggcacgcg actcgttacg gcggcgtagc ccccgtcgag cgggaagtcg atccccatcc
     5941 ccttctggat gaccgtgtag tactgcccgc ggtcgagcac gatatctccc ccgactacca
     6001 cgcgccgcag ctcttgggga tcagggaccg tggcaccatc cggcctcagc ggatagtctc
     6061 cgctaccctc cgagtccagt agcgacctac catctaccga cagtgctttg acccttggac
     6121 caacctcgtc ccagggtacg agcccgagtg cctcgggagt cttgctcacg tgctctacca
     6181 cggcgtcccc cgaatcgaag ctttcgaacc tcgggctgtc taggagctct tcggcctcat
     6241 cacggtaccc tcggccgacg gccacatttc ttgtccccga aagctcttgc gtgctgatgt
     6301 tttccgcggt cgaactcagg tgggcgatcg ggacgatggc cgcctgtgtt tcggcgcggc
     6361 tggcgccttt cgagccgtct ccggtttgcc ctccgcgcgt cgcgctgtcc cgctggccct
     6421 cctcggttcg tcggcccccg tcggaatggt ctttggaccc ttcagaggaa caggcgaata
     6481 gaagtggaaa gagggagatc atgactgcga tcacagccat cgtcaccgct gtgcggtgcg
     6541 tcccctgcgc gtgttgcgcg ttctcgggcg gctctatcat caacgcttga tatagaccca
     6601 ggtcctaccc gccgcccacc gtgcgctcag ccttaagata ggcgccacgg gaggtgaggg
     6661 taactcaggc gcctacgctc agtgggccat gggacgccga cagcgctccg gccttccctc
     6721 ccagcggccc tgtactcgtc ttccgttgcg acccagatca tcacatctcc cgtttcggcc
     6781 gtccagggtt cagagagcgc cctgaagccc ttgcgccacg agccatcttc ggtacgcagg
     6841 tctacgtgct gcccagggtc gggaatgcga ggtatcctct tcaatagagg tagccccgac
     6901 cactgcgccg agccccctaa ccgaaccacc cgcgcccccc ggatgtttgc ccggcacccg
     6961 ccgaagacgc tcttctggcg ggatctagcc aggcgtatcc cgcaagggca gcgaacagta
     7021 cccccagcag cacgagcggc tcgtcccatc cggcaggcgc caacgcagcc aggaatccgt
     7081 acaaggcaag cacagcgaaa atggcgggcc acatccccca cttcggcggc tttggtcggc
     7141 gccggtaaac caggtaggag aggacgaccg cagccatgat cacgattacg ttgggcacgc
     7201 gggtcaaggt gatcacgacg aaatcgtaca cagtcctacg cgctgcctcg cgcacgagac
     7261 ctacacctga agacgtgccg ccaccagcag cgcctggatc cgtcattagc gttctcaccg
     7321 ttctccggga gcctggccaa tcctgcaaca gcctccatga ccgtgtacca acgcttcaat
     7381 gtcttatccc ttcccgcaac ctcgcgcgtg gcaccttgtc gtatcccctt taggtaagga
     7441 tgatactcgc tcccagggag gcttgcgatg gcccacgaga tggggtgcat gccgtccacc
     7501 ccgggcacga gatgtcgtga tctctaacct ttgaccagaa gtaggggcct gatcgagcgc
     7561 ccggtgctcg gtcccggctg cggaacggag gtctcgacga tgacgtgttg ggacgccaaa
     7621 tcgcgatgga acgtcacgac tgcgcgcgct ttatcgaaca cgcgaccgcg gcccgcatca
     7681 cgcttccatt ggcaaccgga cctggaagcg ggtgtcgccg ggtttagaga cgatgcggat
     7741 gtccccgccg tgtctgccga cgacgatccg gtagctcacg tccagaccga aacctgcgcc
     7801 cttgccgact tccttggtag tgaagaacgg ttcgaatatg cgatcttgga tctcctcggg
     7861 gatgccggct ccgttgtccg agatctcgac cagcaatcga tcgcgttcgt aggtcgtgcg
     7921 gagccttatg cggccggtct tgtccccgtc ttccacagcg tctatggcgt tgtctatgag
     7981 gttcgtccag acctgattga gctcgctccc gtgggcgctg atgcggggca gactctcgtc
     8041 atagtcgcgc tcgacctcga tgccagttag cctgtatctg agaatcgcga gagtgttgtc
     8101 gagaccttcg tttacgtcca cttcctgtgt tggtgatctg tccatatagg aataggcctc
     8161 catggtggcg accagattcg agatgcgcat cgaactcgct tcgatctcgt ctacgagcct
     8221 cgccgtcccg agtaccgcct ccaggtaccc cagcgcgtcg gtgagcgtga tgggcggcac
     8281 ggccgattcc acgagtttca gctcagcgat gccaagcccc gcgtcgacga gggtagggga
     8341 gagatcccac gcctccccga cgccacggtc ctcgagccat agggcgacct cgtcctctaa
     8401 atcactccgc tcgagcgaac tcagtgccgg agcattatcg cgcccgaagg cttcgtggac
     8461 gatccgttcc agggcgtcga gctgcaccgg atcgatctcc ccccgggccg ccgcccgggt
     8521 caaccttatg cccagggagc gccggttctc caagctctcg cgtagaagtc cagcggcccg
     8581 gcggcttgcg gatgaagggt tgttcagttc gtgagcgagg ccagcggcga gggtgcccaa
     8641 agagctgagc ctctcgcgct gctgagccac cgaatagagg acctggatgc gctgggccat
     8701 catctcgagt acggtattgc tgaaggaagg ataggtcgca agcatccgcc gaaaggtctc
     8761 gttgggcagc gcaaacaccc tggagttgac gagcgcccgc cacgtggcca ggaagggttt
     8821 gcccaggaga agcggtacct ccgcgaagaa ctcgccggag ccgtaggtgt tcatgaccac
     8881 ctcgccgccg tccactttct tgctccacct gaattctcct tcgaggatca cggacaaatg
     8941 atcgactggc tcaccttctc gccctccgac ctcgccggcc agtatgaact cctccgagcc
     9001 ctcttcgagt atctcccgca attgctcgtc ggtcaatccc tcgaagagcg gcaccttgcg
     9061 cagttcttca agggtcgtca cggtgaactc tcattggcgg gaatcatcgg gaagcctacc
     9121 tgcatgcgcg tgtcgcccgg ctcggagcag acgcgaagag cgccgcagag cttctcgacg
     9181 atcccgaggg ccgcacatat ccatctgcgc tccgaagggg gactctaccc gtggtgcgct
     9241 ggacatctcc gtcaccttct acatctcatg tgtggagctt cttgtaagta tgatgccatt
     9301 caccgcagcc cgatgccatc cccgctgcct acgcgtcatc taatttccga gaacgccccg
     9361 accccgacgt cttcctcgct taccttctac accttatccc ctctgtactc gacgtggatg
     9421 tagacccacc catcggggta ctctttggcg aggacttcgg ctctcccgag cgcctcagcg
     9481 ccgtcggtga ggacggtggt cccgccgtac tcatgcttga tgggccgctt ctggcgtgcg
     9541 tcctcataag cgaggatgta gtagcggtag gtgtctttca tatctcatcc ccggcttcgc
     9601 tagacgatat gccgctctcg ttgtgctctg ggcatggaca ccagatactc atcttcagag
     9661 actctatcct ctcaggtgcg cgagagatgc tccacttgcg gctgttgtcg tcaccgagcg
     9721 cgtgccggct aacgcctact acaacaacgg cgcttcagtt tcgcattggg gtggctgatg
     9781 ccgagggtgg cggcgaccgg ggccaatcca cgctcgatgt gggagagtag gcatcatcaa
     9841 gcggtggagg gttagaagtc tcctccaccg aagtcacctc cgccccaatc gccaccgccc
     9901 cagtcaccgc cgccccaatc gctgccgaaa cctccggtgt cgaaggatga agggtcgccc
     9961 atgtgcatgt aatcgttgtc cagggcctcg aagagtagca gcgcggcgag ccagtctccc
    10021 ccgaagaaac cgtagcgggc gttccccagc tggtactcgt actccctgag cctctgccgc
    10081 tggtggggat ccatgaggtg ctcgttcgct ttcgcctgcg ccagacgtgc ttcgagctcc
    10141 tggagcttct cctcgccctc acgtttctta cggtcggctt ccgccgtggc cgccttcagc
    10201 cggccagggg accgcatggc attccgggca agctcggcgg cttcatgcag cagggagcga
    10261 gcttcgctga agcgtccgga agacgcggcg cgatctgctc gatccacata cccttcggct
    10321 tcgagcaggc tcgaggaaag ctctccaggg ttcggaacct ctccgagcgc ggcctggccg
    10381 aactcgtcaa gcgctctatc gtgggctcca cgatactcct cgagagtttc tcgcgcccgc
    10441 actatcgccc cttccgtcgc gtagcgttcg tcggggagtg cgtcgcgcgc ggagatctcg
    10501 ttctgtagcg ccttgctgtg cgcgcgggcc tgctccgcga gagcttcgac cgccgcgagg
    10561 ctatgccggg gatcgaactc gtccctgcgg gaggcgcgat ccgcggcctc tcgggccacc
    10621 cggtcgtact ccgggcgcag cttcagaggt tcgacggcga tgccctcggc ccgcgcgatc
    10681 tcctcggctt cttcggcggc ctgtatctcc tcctttgcca cacgaagtct gccgtccaga
    10741 gcttcgtcgg cggccctgta gtcgtctatg gttctctcgg cttcgtggag ccgctgcata
    10801 gactctgcga ggaggttgcg cccgtgtccc acacgctcgc gcgccgaact ggggtcggag
    10861 gtgagcctgg atgcggcctc atcgaactcc tcggcgaaac cggtagtctt ggcgtccgag
    10921 atcctcgact ctacccactc ctccgttcgc tgatccaaca gcgatcgctg ggcctccagg
    10981 tagcctgagg cgagccgctc cttctcgtcg aactcgttca ggtggacagt gaggttggcg
    11041 aactcttcct cagcgctccg acgttcttct tcgatgcgcc gtttgttggc gcgccgcttc
    11101 ctgaacaata ggagagctcc tgctaccgcc a
//
LOCUS       DASTKM010000282         7437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_990521, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000282 DASTKM010000000
VERSION     DASTKM010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990521"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFQ10_11380"
     CDS             <1..261
                     /locus_tag="VFQ10_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET9965987.1"
                     /translation="ADGLTGEEGTFSLCTFWLVEALTRAGRLEESRLIFEHMLGYANH
                     LGLYAEEIGHHGEALGNFPQAFTHLTLISAAYNLDRALGRRG"
     gene            361..1755
                     /locus_tag="VFQ10_11385"
     CDS             361..1755
                     /locus_tag="VFQ10_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HET9965988.1"
                     /translation="MAAQHYDAVVIGAGQAGVPLSQALARSGRRTALIEREHVGGTCI
                     NEGCTPTKTMVASAKTAYVDRRSADYGVHNGPVAVDMPEVRGRKRAMVDSFRNSNLRR
                     IEATDGLDLIAGEASFTGPKALAVRTNDNQDLELGADNVFINAGARPANPPIEGLDDV
                     PALNSTSIMELDVLPEHLLVLGGSYVGLEFAQMFRRFGSEVTIVERTGQLMGREDADI
                     AEAVAGILREDGIEVLLEAKTRLAAQDDTTKILLTVDTSEGERTLEGTHLLVAAGRPP
                     NTETLNLGAAGIETDRRGFIKANECLETSVPGVYALGDVKGGPAFTHISYDDFRIIRT
                     NLLEGGRATIADRPVPYTMFIDPQLGRIGLFEQEARAQGRDVRIATIPMTYVTRAVEM
                     GETRGFMKAVVDAETAQILGCAVLGIEGGEIMAMIQIAMMGNLPYTVLRDAVFAHPTL
                     SESLNTLFSSVEHH"
     gene            1882..3090
                     /locus_tag="VFQ10_11390"
     CDS             1882..3090
                     /locus_tag="VFQ10_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9965989.1"
                     /translation="MMPEISLVSVLIVAAVAFVVPLILGLVPALRVPSVVIEIVAGIV
                     IGPAVLGLVEVDGPLRFMALIGLAFLLFLAGMEIDLGGLLRGGAIRSAGIGFVLSLAI
                     AFGIASLLGVAGLIQAPLLVAIMLSATSLGIVVPVLADAGQSRSPLGQLIIAGSSVAD
                     FGAVILLSFFFSGDSSSVGSTLLLIGAFVVLVAAIGIAIAEIEHLGRLSMILRSLQDS
                     SAQIRVRGALLFLVGFAVLAQLLGLEIILGAFIAGTVLRLVDKDGMMTHTLFRPKLEA
                     VGFGVFIPFFFVTSGIQLDVGALLAPGGAALASVPVFLFTLLLVRGLPAILYRPVIGT
                     RGVLAAALLQATSLPFIVAATGIGMELGILSPATGAALVVAGLLSVVLFPVAALTLLR
                     GDKRPSAQQR"
     gene            complement(3129..3980)
                     /gene="folD"
                     /locus_tag="VFQ10_11395"
     CDS             complement(3129..3980)
                     /gene="folD"
                     /locus_tag="VFQ10_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET9965990.1"
                     /translation="MAKILDGRTAAAEVRSEVASEVAGLRELGVPVRLDVILVGDDPA
                     SITYVASKERDSEEVGIESRVHRFSAGVSQKELSGLVGRLNEDTAVSGFFIQLPLPEE
                     LDSMPLISSITPAKDVDGLSPESAGRLAVGLPSLLPCTPHGVIQLLKRNGIDLSGREA
                     VVVGRSNLVGKPLAQLLLRENATVTVCHSRTRGLPEVTRRADILVIAAGKREMIGAEH
                     VGEGAVVVDVGIHRKEDGGLVGDVRFDEVDQRASWISPVPGGVGPMTRAMLLHNTVVA
                     ARMSLGS"
     gene            complement(3988..5613)
                     /locus_tag="VFQ10_11400"
     CDS             complement(3988..5613)
                     /locus_tag="VFQ10_11400"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HET9965991.1"
                     /translation="MPHVYVTGHKNPDTDTIASAIGYAEYKNLVDPENHYVAARLGEV
                     NAQTGWALKKSGAEKPGLLEHIMLRVKDVMAGDVAVARKNDPLRNVGLTMARRNISQL
                     PIVEDDGSLTGIITERNLARMYVRESRGASTFADSPVSVGAMVEVLEGELLVGEADRE
                     SSGQLWVISMSVDSMGQSMEPGDIVVIGDRPRGQRRAIELGAGVLVVSNGVRPEDEVL
                     KVARERGTAVLLSPLDSYVTSRLIQLSVPCWEVMSENPLTVRPDDLVTEITEQVMEVH
                     YRAAIAVDEKRVPIGVVTRTDLLNPRPRRVLLVDHAEIGQSVAGVEKAEIVEILDHHH
                     IGDIETTSPIPATFDPVGSTATLIVERFKASGLRPEEPTAKMLLAALLSDTVILNSPT
                     TTDRDREVARYLEEMLDLDANEFGTEMFEASSDVSSLSAQEIVGRDAKEYGTSSGNKV
                     SVSQVEIVGTGLLERKDELMDALEDLKGENGYLFSALMVTDIIEGGTQLLCVGDCSYV
                     HQAFDSQAQNGVIELPGVMSRKKQVAPLLLSVL"
     gene            complement(6040..>7437)
                     /locus_tag="VFQ10_11405"
     CDS             complement(6040..>7437)
                     /locus_tag="VFQ10_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET9965992.1"
                     /translation="VYSWRGADIRNILDFERDYPEAKVVKLEQNYRSTQTILDAANAV
                     VANNASRKAKALWTAGGEGERIRVFAASDEYAEARFVVSEVERLIEGGASPREISVFY
                     RTNAQSRALEDVLVREGVPYQVVGGVRFYERAEIKDAMAYLSVISNPDDSVSLERIIN
                     VPKRGLGDTSVAKLQDYARRNGLSLYGALSETDAVGLTGAAKKACRGVRDLFEGLRVA
                     AMEVPPAELIGAVLDESGYRKELEAEDTVEAESRLENLEELINAAREYERVELEPTLE
                     GFLQEQALYSDQDSLTSEGGRVTLMTLHNAKGLEFGHVFVVGMEEGTFPHARSLDEHN
                     LEEERRLAYVGITRARETLTLTHAKLRSSWGEREYRMPSRFLSEIPDEYKSGTVPTLA
                     NASGRGGWGVALPGRGGPERAAPGGTAYKTGEKVRHTKFGVGEVVEAGVGKVVIRFGT
                     EERTFVPEIAPLSKV"
BASE COUNT         1306 a         2433 c         2250 g         1448 t
ORIGIN      
        1 gcggacggcc tgacgggcga ggaagggacc ttcagcctct gcacattctg gctcgtcgag
       61 gcgctcacgc gggcgggccg actggaggaa tcccgcctca tcttcgagca catgctcggc
      121 tacgccaacc acctcgggct ctacgccgag gagatcggtc accatggcga ggcgctgggc
      181 aacttcccgc aggccttcac gcacctcacc ctcatcagcg cagcctacaa cctcgatagg
      241 gcgctcggca gaaggggtta gtgcactgaa gaagtaaacc tatggacggc cagggtagcc
      301 taaacaaaca tccatcagcg ctcggcgcga acaagcacca agtgaaagga gactgctttt
      361 atggcagcac agcattacga cgccgtggtg atcggggcag gccaggccgg ggtcccgctt
      421 tcccaggcgc tcgccaggtc gggacgtagg acggccctga tcgaacgcga acacgtaggc
      481 ggcacctgca tcaacgaggg ctgtacccca accaagacga tggtcgccag cgcgaagacg
      541 gcctacgtag accggcgttc ggccgactac ggcgtccaca acggtcctgt cgccgtcgac
      601 atgcccgagg tgcgaggccg caagcgggcg atggtggaca gcttcaggaa cagtaacctg
      661 aggcgcatcg aagccactga tggacttgac ctgatcgctg gcgaagccag ctttacgggc
      721 ccgaaagcgc tcgcggtacg cacgaacgat aaccaagacc tcgagctcgg cgcggacaac
      781 gtctttatca acgctggcgc caggccggcc aaccctccga tcgaaggtct ggatgacgta
      841 ccggccctga actcgacctc catcatggag ctcgacgtcc tgccggagca cctgctcgtg
      901 ctcggcggca gttacgtcgg gctggagttc gcccagatgt tcaggcgttt cggcagcgag
      961 gtcacgatcg tagagcgaac tgggcagttg atgggccgag aggatgccga catagccgag
     1021 gcggtagccg ggatcctgcg cgaggacggt atcgaggtcc tgctggaggc caagacccga
     1081 ctcgccgcgc aggacgatac tacgaagatc cttctgacgg tagacacatc cgaaggggag
     1141 cgcacgctgg aaggcacaca ccttctcgta gcagccggca ggccgcccaa caccgagacc
     1201 ctcaacctcg gtgcggccgg catagagaca gacagacgcg gctttatcaa ggcgaacgag
     1261 tgtttggaga ccagcgtacc cggcgtctac gccctcggag acgtgaaggg gggacctgcc
     1321 ttcacccaca tctcctacga cgatttccgc atcatccgca ccaacctgct ggagggtggc
     1381 agagctacca tcgcagaccg ccctgtcccc tacaccatgt tcatagatcc acaactaggc
     1441 cggatcgggt tgtttgagca ggaagccagg gcccagggcc gcgacgtgcg catagccacg
     1501 attccgatga cctacgttac gcgggccgta gagatgggcg agacgcgtgg cttcatgaag
     1561 gcagtcgttg acgccgagac cgcccagatt ctgggctgcg cggtgctcgg gatagagggg
     1621 ggcgagatca tggcgatgat ccagatagca atgatgggca acttgccata cacggtcctc
     1681 cgcgacgccg tattcgccca ccccacgctc tctgagtcac tcaacacact cttctcgagc
     1741 gtggagcatc actgaggagc gagtctggcg ttccgtcgcc cacagtggcg ccgcgtatcc
     1801 gcgacagcga ggagatgata cggttcttca ccaaggcacg tctgactacg gagccataaa
     1861 gcctaagcca ggagaaccct gatgatgcct gaaatctccc tcgtcagcgt gctgatcgtc
     1921 gcagcggtcg ccttcgtagt gccgctcatc cttggcctgg taccggccct gcgcgttccc
     1981 tccgtcgtca tcgagatcgt agccggcatc gtgatcggtc ccgccgtcct cggtctcgtc
     2041 gaggtggacg gacctttgcg ctttatggcc ctgatcggcc tggcgttctt gttgttcctg
     2101 gcggggatgg agattgatct cggcggcctg ttgcggggtg gggcgatacg tagcgccggc
     2161 atagggttcg tgctgtcgct ggcgatcgcg ttcggcatcg cctcgttgct cggtgtcgcg
     2221 gggctcatac aggctccgct gctcgtggcg atcatgctct ccgccacctc gctcggcatc
     2281 gtcgtccccg tgctcgccga cgccgggcaa agccgctccc cccttgggca gttgatcatc
     2341 gccggttcat cggtcgccga tttcggcgcc gtgatcctgc tctcgttctt cttctccgga
     2401 gactcgagca gcgtcgggtc cacgctcttg ctgatcggag ccttcgtcgt gctcgttgca
     2461 gccattggga tcgcgatcgc cgagatcgaa catctcgggc ggctgagcat gatccttcgg
     2521 agcctgcagg actcgagcgc ccagatccgg gtgcgcggcg ccctgctttt cctggtcggc
     2581 ttcgccgtgc tggcgcagct tctggggctc gagatcatcc ttggagcatt tatcgcaggc
     2641 acggtcttgc ggttggtgga caaggacggg atgatgacgc ataccctctt ccgaccgaag
     2701 ttggaggccg tcggtttcgg cgtcttcatc ccgttcttct tcgtcaccag cggtatacag
     2761 ctcgacgtgg gagcacttct cgcccctggc ggagcggcac tcgcttctgt gccggtcttc
     2821 ctctttactc tgctgctcgt ccgcggactg ccggcgatcc tgtacagacc tgtgatcggc
     2881 accaggggcg tgctggcggc ggcgctactg caggcgacct ccctgccatt catcgtcgcg
     2941 gcaacgggca tcggtatgga gctcggtatc ctgagccccg cgacgggcgc tgctctcgtg
     3001 gtcgccggcc tactctcggt cgtactgttt ccggttgccg ccctcacgct cttgcgaggc
     3061 gacaagagac cgtcggcgca acaacgttga tgaggccgcc gtccatgatg ttctgcgctg
     3121 aatctctcct agctccctag gctcatccgc gcggccacga ccgtgttgtg gagcagcatc
     3181 gcacgggtca tcgggccgac gcccccaggg acgggggaga tccaggacgc tctctggtcg
     3241 acctcatcga agcgaacgtc cccgacgaga cctccatctt ctttgcgatg gatgccgacg
     3301 tcgacgacga ccgcgccctc cccaacgtgc tcggccccga tcatctcccg cttgccggcg
     3361 gcgatgacga gaatgtcagc cctccgggtg acctcaggaa gaccccgtgt acgggagtgg
     3421 cagaccgtga cggtagcgtt ctcgcgcagg agtagctgtg cgagcggctt gccaacgagg
     3481 ttcgagcgcc cgacgacgac agcctcccgc ccggagagat ctatgccgtt gcgcttcagg
     3541 agttggatca cgccgtgcgg cgtacaaggg aggaggctcg gcaggccgac ggcgagccgt
     3601 cctgcgctct cgggactgag accatccacg tccttggcgg gggttatgct cgagatcagg
     3661 ggcatagagt caagctcttc aggcaggggg agctggataa agaagcctga gacggctgta
     3721 tcttcgttca accgccccac gaggcccgaa agctctttct gggagacgcc agcggagaag
     3781 cggtgtaccc tcgactctat cccgacctcc tcgctgtcgc gctccttgga ggcgacgtag
     3841 gtgatggagg caggatcatc gccgacaagg atcacgtcga gccgcacggg cacgccgagt
     3901 tctcttagcc cggcgacctc tgaggcaacc tccgagcgga cctcggccgc agcagtcctg
     3961 ccgtccagta ttttcgccac gcgtctccta aaggacggag agcagtaagg gggcaacctg
     4021 cttcttgcgg ctcatcacgc ctggcagttc tataacgccg ttctgggcct gagagtcgaa
     4081 ggcctggtgc acgtaggagc agtccccgac gcacaagagc tgggtgccgc cctctatgat
     4141 gtccgtgacc atgagcgctg agaacaggta gccgttctcc cctttgaggt cctctagggc
     4201 atccatcagc tcgtccttgc gctcgaggag gccggtaccc acgatctcca cctgggagac
     4261 gctcaccttg tttcccgagc tcgtcccgta ctccttcgcg tcgcggccaa cgatctcctg
     4321 tgcggagagc gaagagacgt ccgaggaggc ctcgaacatc tccgtcccga actcgttcgc
     4381 gtcaaggtcg agcatctcct cgaggtatcg agcgacctcg cggtcgcggt ccgttgtggt
     4441 cggggagttc aggatcaccg tgtccgagag gagggcagcg agcaacatct ttgcggttgg
     4501 ttcctcgggt ctcagaccgc tcgccttgaa gcgctcgacg atcagggtgg ccgtcgagcc
     4561 gacgggatcg aaggtagcag ggatgggaga ggtcgtctcg atgtccccga tgtggtggtg
     4621 gtccaggatc tcgacgatct cggctttctc gacgcctgcg acgctttgtc cgatctctgc
     4681 gtggtcgacg agtagtacgc gcctcggtct ggggttgagg aggtcggtcc tcgtaaccac
     4741 cccgatgggc accctcttct cgtccacggc gatcgccgcc ctgtagtgga cttccatcac
     4801 ctgctcggta atctccgtta ccaggtcgtc tggtctgacg gtcaggggat tctcgctcat
     4861 gacctcccaa caggggacgg agagctgtat caggcggctc gtcacgtagg agtcgagggg
     4921 agagagcagt acggccgtcc ccctctccct ggcaaccttc aggacttcat cctcaggacg
     4981 aaccccgttg gagacgacga gtacgccggc gccaagctct atggccctcc gctggcccct
     5041 ggggcggtct ccgatcacca cgatgtcgcc aggctccatg gactgcccca tcgagtccac
     5101 gctcatcgag attacccaca actggcccga ggactcacgg tctgcctccc cgaccagaag
     5161 ttcgccctcg agaacctcga ccatcgcccc gaccgagaca gggctatcgg cgaaagtgga
     5221 agccccgcgc gactcccgca cgtacatgcg cgccaggttc cgctccgtga tgatcccggt
     5281 gagagagcca tcgtcttcca ctatcgggag ctggcttatg ttccgcctgg ccatagtaag
     5341 gccgacgttg cgcagcgggt cgttcttgcg ggcgacggcc acgtccccgg ccatgacatc
     5401 ctttacgcgc agcatgatgt gctccaacag gcccggtttt tcggccccgc tcttcttcaa
     5461 agcccaccct gtctgggcgt ttacctcacc gagccgcgcc gccacatagt ggttctcagg
     5521 atcgacgagg ttcttgtatt cggcgtatcc gatagccgag gcgattgtat ccgtatcggg
     5581 gttcttatga cccgttacat agacgtgagg caaggcatcc tccattcact ttacctgagc
     5641 ctattgcgcg ccgagtacta tccgtcttga tgcctacccg acaccaacgt aagagtattt
     5701 cactgtgaaa agagtctacc agagagccta aaatccgtac gtgtcgatca gatccgcggc
     5761 catttgctgg tcatcggaga gtgtgtgagc ataacgtctt gggttcatgg cggggtcttt
     5821 atggcccaag acctgggaca ccacgttgat cgggaggccg gagtcgagca tcagtacggc
     5881 cgcagtatgc cgcagatcat ggaagcggac ctgaggcaac caggccgcct cgcgatgagt
     5941 ctggttcgcc acttcacctt cgttttgttc ccccctgtcc gcgctcactg gcagttggcc
     6001 ctatcgaggc atgaactatg ccagggaggg gaatttcggt caaaccttgc tcaatggagc
     6061 tatctccggg acgaaggtcc tctcctcggt cccgaacctg attaccacct tcccgactcc
     6121 ggcctccacg acctccccga ccccgaattt ggtgtgcctg accttctccc cggtcttgta
     6181 tgccgtccct cctggggctg ccctctctgg ccctccacgc ccgggcaaag ccacgcccca
     6241 tcccccgcgg cctgaggcgt tcgcaagagt aggcaccgtg cccgacttgt actcgtccgg
     6301 gatctcggag aggaagcgag agggcatcct gtactcgcgc tcgccccaac tggagcgcag
     6361 cttggcgtgc gtaagcgtga gcgtctccct ggcgcgcgtg atgccgacgt aggcgagcct
     6421 ccgctcctct tcgaggttgt gttcgtcgag cgaacgggcg tgcgggaagg tcccctcctc
     6481 catccccacc acgaagacgt gcccgaattc gagccccttc gcgttgtgga gcgtcatcaa
     6541 cgtcacgcga ccaccctctg aggtcagaga gtcctggtcc gaatacaggg cctgctcctg
     6601 caaaaagcct tcgagcgtcg gctcgagctc gacgcgctcg tactccctcg cggcgtttat
     6661 tagctcctcg aggttctcca ggcgcgactc ggcttcgacg gtgtcctcag cctccagttc
     6721 cttcctgtat ccagactcgt cgagtacagc cccgatcaac tccgccggcg gcacctccat
     6781 tgcggccact ctaagcccct cgaagagatc gcggacaccc cggcaggcct tctttgcagc
     6841 acccgtcagg ccgacagcgt cagtctccga gagcgccccg tacagtgaga ggccattcct
     6901 gcgcgcgtaa tcctggagct tcgcgaccga ggtgtcaccg aggcccctct tgggcacgtt
     6961 gatgatccgc tccagggaga ccgagtcgtc ggggttggag atcaccgaga ggtatgccat
     7021 ggcgtctttg atctctgccc gctcgtagaa cctgaccccg ccgacgacct ggtatgggac
     7081 gccttcgcgc acgagcacgt cctccagcgc cctgctctgg gcgttcgtcc tgtagaagac
     7141 cgagatctcc cgcggagaag ccccaccctc gattagccgc tcgacctcgg agacgacgaa
     7201 cctggcctcc gcgtactcgt ccgaagcagc gaacaccctg atccgctctc cctcgcctcc
     7261 ggcggtccac agcgccttcg ccttgcgcga ggcattattg gccaccaccg cgttcgccgc
     7321 atccagaata gtctgcgtcg aacggtagtt ctgctcgagc ttgacgacct tagcttcggg
     7381 atagtcccgc tcgaagtcca aaatgttcct tatgtcggct ccgcgccacg agtacac
//
LOCUS       DASTKM010000283         5443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_814415, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000283 DASTKM010000000
VERSION     DASTKM010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814415"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFQ10_11410"
     CDS             complement(<1..932)
                     /locus_tag="VFQ10_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9965993.1"
                     /translation="MREVQPPGDASERFSGMEHKEDRDKHRRPGRLLAVFKGLVMLAL
                     LAATAYGMLDAGLYRDELWLPVAAGVLALLFVTLFVRGFYWDLPSVGILLVGLLAALV
                     AVKGLSMVWTISETETIKETLRSSMYLAAFLMALAALTSGRQVGSLIDASVFMVAAVA
                     GYGLLQKISPIEYPVRSLDGIRMDSTLQYSNTTAVLLGMGVVLALARMSTLRNFLLRG
                     LYAAVTLSLFIALYLTVSRGGIGSLGIGLIVLFIITSGRLRMFADIILLSLPGAWLWW
                     QMQSLPGLLGTGVSRQQKIADGTLFRNDLILALIV"
     gene            complement(958..1590)
                     /locus_tag="VFQ10_11415"
     CDS             complement(958..1590)
                     /locus_tag="VFQ10_11415"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HET9965994.1"
                     /translation="MRLGAISDAGARGLLDPDSPVRRYYEELAGNLLRMRGTGSVVVT
                     SPEPGTGRTSICFGLGAALAGMGRRAAVVDCNLENSQLHRMLGEPNFVGLTSGLDDSK
                     SLEHFGYEVIPGFLVVPTGPILSDPASLLEDEGVPKAVRGLQKSRDLVLLDAPVASRV
                     LRSPNLLGGFDGVLLVVHASRTPKSVARETTDDLLDAGANLLGVVLNGCS"
     gene            complement(1592..2317)
                     /locus_tag="VFQ10_11420"
     CDS             complement(1592..2317)
                     /locus_tag="VFQ10_11420"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HET9965995.1"
                     /translation="MTSTSGRGWRDKAREDPSLSEILYVLQRRRLLIVGVVLVLAGIA
                     LLFGLFREPVYTAEAKVSVRPQVKLDDEAMLAFLEEVRGAVVTQEMLSEVRRRAGWEA
                     EPKAFSERLDPRTVADSDGGPGLQIRFHGDDPGQAALAANAYAELFVERVEDLGDRPP
                     AGGVQVAEALVEQKALPGDYRPRPLIYAAIAAGVGLLIGGAAALILEGRASGWRGVRD
                     AELTLRVPVLGTIPDYSSKRTEG"
     gene            complement(2304..3095)
                     /locus_tag="VFQ10_11425"
     CDS             complement(2304..3095)
                     /locus_tag="VFQ10_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9965996.1"
                     /translation="MIFRLSAGFLVGVLALLAAVLYLSDYYTAEQQRLAAAGDVRGAM
                     EASRKAVRLDPFDTEALQAQSFLWRQQQKQGRAVLALKEAIERDPNNYLPYLTLANLR
                     LGMGDLDAAAKGYRNVLELNPNAVSAKGSLAQTLVRQGKLQEAKEKYEALEEEKNISY
                     QDRYDLGRIQVRTGEPLEGVKNIRRARRLAAAELKRPRTQAGDRQLLVSMDLALADAF
                     VVQGRYGQARKILAQSPSEQAPGLLQLLNLDPAAYREQVINSDIY"
     gene            complement(3229..3651)
                     /locus_tag="VFQ10_11430"
     CDS             complement(3229..3651)
                     /locus_tag="VFQ10_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET9965997.1"
                     /translation="MKKVMLLAAMLAMVLAAAVPALAQSVTQVGSGNQACVQTAIQAE
                     VGKVQISQYGDNVNIQNIAQVCNVGVPVVEEVVAAASPAAAPAAVQYQYPAAAQYQYS
                     APASPAAELPPTGGSSLIALGAGVLLVAGGLLARRIVR"
     gene            complement(3772..4314)
                     /locus_tag="VFQ10_11435"
     CDS             complement(3772..4314)
                     /locus_tag="VFQ10_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET9965998.1"
                     /translation="MRCMLVVVFVAILLAAADLIRADSPAEAAGGGYASRCGGGKIFL
                     YATEERLLTLHNNARENHGLKPFCVHPALQKAARAHSKDMIQYDYFSHDTRGRNEDPC
                     TRIRRFGYRSSYCTENIGYNATPEGVFRSWMRSSIHRPNILDGRFHEIGIGACTGDYS
                     DSKTTMYTTDFAAPAEQRSE"
     gene            complement(4488..>5443)
                     /locus_tag="VFQ10_11440"
     CDS             complement(4488..>5443)
                     /locus_tag="VFQ10_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9965999.1"
                     /translation="EPVARLEIPTVGSGLEAPTTGSQTGSNPDPRAASEVAKGATRVE
                     MELTEEKTESLRSELGQIAQAYPATYGVVVLDPSSGERVAMDADRRFLAASLNKLPIL
                     MTLYRAAASGVVDLDDKISMQASDVQAYGSGVLYTYPVGHTMTLRECARFLIKESDNT
                     AWKMLDRYLGRDYIKAELYRVGARSTEYWFPNTTTPDDVLLVLEKISDPSYTTPHLSA
                     EMLEVMTNTSFEERLPQPLPEGTLVSHKIGYYGTTFADAGIVFPEGARDARDAYLMVV
                     MASDTSESASRVATQEMSLATYRILSEPAQTDASGSKEEGS"
BASE COUNT         1061 a         1737 c         1621 g         1024 t
ORIGIN      
        1 acgatgaggg ccaagatcag atcgttcctg aacagcgtcc cgtcggcgat cttctgctgg
       61 cgtgagacgc cggtcccgag taacccaggg aggctctgca tctgccacca gagccaggcg
      121 ccgggcaacg agaggaggat aatgtcggcg aacatccgca ggcggccgct cgttatgatg
      181 aagagtacta tgagtcctat tcccagcgac ccgatgccgc cgcgagagac cgtgaggtag
      241 agcgcgataa acaaagagag cgtgacggcg gcgtagagtc ccctcaggag gaaattgcgc
      301 agcgtactca tccgcgccag ggccagcacc acccccatcc ccaggaggac ggcggtggtg
      361 ttggagtact gcagggtcga gtccatcctt atcccatcca gggagcgaac cgggtactcg
      421 atcgggctga tcttctgcaa gagtccgtag cctgcgaccg ccgcgaccat gaagaccgag
      481 gcgtcgatga gcgacccgac ctggcgccct gaggtgagcg cggccagtgc cataaggaag
      541 gccgccaggt acatagacga gcgcaaggtc tccttgatgg tctccgtctc acttatcgtc
      601 cagaccattg agaggccttt tacggcgacg agcgccgcca gcaatccgac cagcaggatg
      661 ccgacagagg gaaggtccca atagaaaccg cgcacgaaca gcgttacgaa cagcaacgcc
      721 agaacgcccg cggcaacagg cagccagagc tcgtccctgt agaggccggc atcgagcatc
      781 ccgtaggccg tggcggccaa gagcgccagc atgacaagac ccttgaaaac ggcgagcagc
      841 cggccaggac ggcggtgctt gtccctatcc tctttgtgtt ccatgccgga gaagcgctcg
      901 ctggcgtcgc cggggggttg gacttccctc actacttcgc cccggggcga tcgcgcatca
      961 ggaacatccg ttcaacacca ccccgagcag gttggcacca gcatccagaa ggtcgtccgt
     1021 cgtctcgcgg gcgacgctct tgggagtccg ggaggcgtgg acgacgagca ataccccgtc
     1081 aaagccacca aggaggttcg gagaccgcag cacccgcgac gccaccggcg cgtcgagcag
     1141 cacgaggtcc cggctcttct gcagacctcg tacagccttc gggaccccct catcctccag
     1201 tagagaggcg gggtccgaca gaatgggacc ggtcgggacg accagaaatc ccgggatgac
     1261 ctcataccca aagtgctcca gggacttact gtcgtcgaga ccactggtaa gcccgacgaa
     1321 gttcggttcg ccgagcattc tgtgcaactg cgagttttcg aggttgcagt ccacgacggc
     1381 ggcccgccgt cccatgccgg ccagggcagc tccgagcccg aagcagatgc tggtccgccc
     1441 ggtgccaggc tcgggactgg tgacgacgac actacccgtc cccctcatcc tcagaaggtt
     1501 gcccgcaagc tcctcgtagt aacgcctcac tggtgagtcg gggtcgagca accccctggc
     1561 acccgcgtcg cttatggccc cgagcctcac atcaaccctc ggttctcttc gaagagtagt
     1621 ccggtatggt ccccagcacc ggcaccctca gcgtcaattc ggcgtcgcgt acgccccgcc
     1681 aaccgcttgc ccgcccctcg agtatcaaag cggcggcgcc ccctatcagc aagcctaccc
     1741 ctgcggcgat ggccgcgtag atcagaggtc gaggtctata gtctcccggc agggctttct
     1801 gctctacgag agcctccgca acttgtacgc cgccggcggg cggcctgtcg ccaagatcct
     1861 cgaccctctc gacgaataac tcagcgtaag cattcgccgc cagggccgcc tgccctggat
     1921 cgtcgccgtg gaatcgtatc tgtaaccctg gcccgccgtc cgaatcagcg accgtcctcg
     1981 ggtcgagccg ttctgagaac gccttcggct cagcctccca gccagcccgg cgccttacct
     2041 cgctcaacat ctcctgcgta acgacggcgc cacgcacttc ttcgaggaag gcaagcatgg
     2101 cctcatcgtc cagtttcacc tgcggcctga cgctcacctt cgcctcggcg gtgtataccg
     2161 gctcgcggaa gagcccgaat agcagggcta tgccagcaag gaccagcacg accccgacta
     2221 ttagcaaacg ccgacgctgc aatacgtaga ggatctcaga gagcgaggga tcttccctcg
     2281 ccttgtcccg ccagccccgg ccgctagtag atgtcactgt tgatgacctg ctcgcgatag
     2341 gccgcggggt ccaggttcag taactgcaga aggcccggtg cctgctcgga cgggctctgc
     2401 gcgagaatct tgcgcgcctg cccgtaccga ccctgaacga cgaacgcatc ggcgagagcg
     2461 aggtccatag agaccaagag ctgccgatct cctgcctgtg tacgcggcct tttcagttcg
     2521 gcggccgcca ggcgcctcgc acgccttata ttcttcacgc cttcaagcgg ctcgccggtt
     2581 cgtacttgga tcctaccaag atcatagcgg tcctgatagc tgatgttctt ctcttcctcg
     2641 agggcctcgt acttctcttt ggcttcctgc agctttccct gtctcaccag cgtctgagcc
     2701 agagaacctt tggccgaaac tgcgttcggg ttgagctcga gcacattacg gtagcccttc
     2761 gccgcagcgt ccagatctcc cataccgagc cggagattgg ccagcgtcag gtaaggcagg
     2821 tagttgttgg gatcccgctc tatcgcctcc ttgagagcaa ggaccgccct gccttgcttt
     2881 tgctgctgcc gccatagaaa ggactgcgcc tggagcgctt cggtatcgaa ggggtcaagg
     2941 cgaacagcct tccgcgacgc ctccatcgct cccctaacgt cccctgcagc ggccaggcgc
     3001 tgctgctcgg cagtgtagta atccgaaagg tagagcaccg cagccagtag ggccaatacc
     3061 cccaccagaa aacccgcgga gagtctgaag atcacacggc tcaagcaaga tcaccgctca
     3121 tgaggatatc cacaacatcc cccgaataca agccgccggg cgggcgaagc cggcacgaaa
     3181 aaagcgggtc cccgaagggg cccgcctggt taggcctcgt ccggtcgctt agcggacgat
     3241 cctgcgagcc agtagaccac cggctacgag caggacaccg gcaccgagcg cgatgagcga
     3301 cgaaccaccc gtagggggaa gctcagccgc ggggctggcg ggggcagagt actgatactg
     3361 tgcggccgca ggatactgat actgcacggc agccggagcg gcggccggac tagcagcggc
     3421 gacgacctcc tcgaccacgg gcacgccgac gttgcacacc tgggctatgt tctggatgtt
     3481 gacgttgtcc ccgtactggc ttatctgtac cttgccgacc tctgcctgta tggcagtctg
     3541 aacgcaagcc tgattgccac tgccgacctg tgtaaccgac tgcgccaaag ccgggacagc
     3601 ggcggccaac accatggcaa gcatagccgc taataacatt accttcttca aaaatctacc
     3661 tcctattcga gatcagcctc tacaggccgc attttttcac ctttagccca cagtgtcaac
     3721 actgttgcct atatcttcta cgattatctt aaaatttctt aataattggg cctattctga
     3781 cctctgttca gcgggggcag caaagtccgt agtatacatg gtagtcttgg aatcgctgta
     3841 gtcgcccgta caggcaccta tgcctatttc gtggaacctc ccatccagta tattagggcg
     3901 gtggatggaa ctgcgcatcc aggagcggaa cacgccttcc ggagtagcgt tgtatccgat
     3961 gttctctgtg cagtaggacg agcggtaacc gaagcgacgg atgcgcgtgc acgggtcttc
     4021 gttcctcccc ctggtatcgt gagagaagta gtcgtattgg atcatgtcct tggaatgagc
     4081 acgggcggct ttctgaaggg cgggatgcac gcagaaaggc ttcaggccgt ggttctcacg
     4141 agcgttgttg tgcaaggtga gaagccgctc ctctgtggcg tacaagaaga tcttaccccc
     4201 tccgcacctg ctggcatagc ctccacccgc tgcctcggcg ggcgagtctg cacggatgag
     4261 gtctgcggcc gcaagaagga tggcgacgaa gactaccacc agcatacacc tcatgaatac
     4321 attccccctc tcgttgttcc agcatttcag ccagtcagaa tttcagctcg tcgttctcgc
     4381 tccttgccga atatgcgata ggtcgataac tctgcacatc gccctgcatg tcagtctatg
     4441 tatcgggaca gcctcccttt ccggcctggc gccggagaca tcgtcatcta cgatccttcc
     4501 tccttgctgc ctgaagcgtc cgtctgtgcg ggctcggaca ggatgcggta ggtagcgagt
     4561 gacatctcct gtgtggcgac gcgggatgcc gactcactcg tgtcgctggc cattacgacc
     4621 atgagatagg cgtctcgggc gtcgcgcgcg ccttcgggga agacgattcc ggcgtcggcg
     4681 aaagtagttc cgtagtaacc gatcttgtgc gagaccagcg tcccttcggg aagaggctgg
     4741 ggcaggcgct cttcgaagga agtgttcgtc atgacctcga gcatctctgc cgagaggtgg
     4801 ggggtggtgt aggaaggatc cgagatcttc tcaagcacga gcaggacgtc gtcaggggtc
     4861 gtggtgttgg ggaaccagta ctccgtcgag cgcgcgccca cgcggtacaa ctcggccttg
     4921 atgtaatccc ttcccaggta cctgtcgagc atcttccagg ccgtgttatc gctctctttg
     4981 atcaggaacc tggcgcactc gcgcagggtc atagtgtgcc caacggggta ggtgtagagc
     5041 acacccgagc cgtaagcctg cacgtcggag gcctgcatgg agatcttgtc gtcgaggtcc
     5101 accacgccgc tggctgccgc cctgtagagc gtcatcagga tgggcagctt gttgaggctg
     5161 gccgcgagga agcgccggtc ggcgtccatc gcaaccctct cgccagagga ggggtcaagg
     5221 acaaccacac cgtaggtggc gggatacgcc tgcgctatct ggccgagctc gctccgcagg
     5281 gactctgtct tctcctcggt gagttccatc tcgacgcggg tggcgccttt ggccacctcg
     5341 gaggcggccc gaggatcagg gttcgagcct gtctgcgacc cagtggtggg ggcctcaagg
     5401 cccgacccga ccgtgggaat ttcgaggcgg gctacaggtt ctc
//
LOCUS       DASTKM010000284         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_990875, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000284 DASTKM010000000
VERSION     DASTKM010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990875"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFQ10_11445"
     CDS             complement(<1..393)
                     /locus_tag="VFQ10_11445"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9966000.1"
                     /translation="MGEWRDEWREANRANWDERVPIHVSGEFYDVASFKRGQERLQPF
                     EIDEVGDVSGKELLHLQCHFGIDTLSWARRGARVTGLDFSAPAVEAARGLASDMGLEA
                     TFVHSDVYDAVDATGGLSFDIVYTGRGAI"
     gene            complement(406..2331)
                     /locus_tag="VFQ10_11450"
     CDS             complement(406..2331)
                     /locus_tag="VFQ10_11450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9966001.1"
                     /translation="MSERGVAPYGSWKSPISSDLIVRGVVGLAEVVIDGRDVYWLESR
                     PGEGGRNVVVLLTPDRRTEDVTPQPFNARTRVHEYGGGGFTVDGGMVYFSNFADQRIY
                     YRAPGGEPAPLTPETGRRYADMVVDRERRRLIAIREDHAGNGPVNEIVGIDLESGEEY
                     ELVSGNEFYSSPRLSPDGRRIVWLTWNHPNMPWDGTELMGCDLGADGFPENVEQIAGG
                     QDESIFQPEWSPEGTLHFVSDKTGWWNLYRWREGRDEPLCEREAEFGLPQWVFGMSTY
                     AFVSPGRIVCTYTESGSSYLAVLDTESGELEPLETPYSSIAYVRADVAAGIVFRGSSP
                     TEAACIVRLDPSTGLHEVLRRSGDLEIDPGYLSVPEPVEYPTEDGRTAHAFFYPPKNR
                     DFAAPDGELPPLLVMSHGGPTAASNTALDPRIQYWTSRGIAVLDVNYGGSTGYGREYR
                     RHLEGAWGIVDVDDCANVAIYLAERGLVDGERLMITGGSAGGYTTLCALAFRDTFAAG
                     ASHFGVSDVEALARETHKFESRYLDSLIGPYPGRADLYVERSPIHSTGHLSCPVIFFQ
                     GLEDEVVPKEQAEKMFAALREKGLPVSYVPFEGEQHGFRQAENIKRALDGELYFYSRV
                     LGFELADSVEPVPIENL"
     gene            complement(2366..2743)
                     /locus_tag="VFQ10_11455"
     CDS             complement(2366..2743)
                     /locus_tag="VFQ10_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966002.1"
                     /translation="MGEHRGSPIERILHRIEERLEDWRKREAALQAEADASRSRLWAE
                     AAERERLLAEAVGAEEARRESIEELTKHHRVVFVLHRTEVEETLQDFVREGDRLVSVV
                     PRRGGETISEGLKGSWLVFESSD"
     gene            complement(2842..>3288)
                     /locus_tag="VFQ10_11460"
     CDS             complement(2842..>3288)
                     /locus_tag="VFQ10_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9966003.1"
                     /translation="YVGAAATKSVILGLIILATAGLFVPLEVAHPLLMVLFLVLTAFT
                     FSLFGFILGIWAEGFEKLQLVPFLIITPLIFLGGSFYSVDMLPPFWQTVTLFNPVVYL
                     ISCFRWSFYGTADVSVGLSLAMTILFLILCLTAVWWIFKTGYRLKA"
BASE COUNT          589 a         1112 c          990 g          597 t
ORIGIN      
        1 gatcgcgcct cggcccgtat acacgatgtc gaaagagagg ccacccgtgg cgtccacggc
       61 atcgtagacg tccgagtgca cgaacgtggc ttccagcccc atatccgagg ccagcccgcg
      121 ggccgcctct acggctgggg ccgagaagtc caggcccgta acgcgggcgc cgcggcgggc
      181 ccagctcagg gtgtcgatcc cgaagtggca ctggaggtgc agcaactcct tgccgctcac
      241 gtcgccgacc tcgtctatct cgaagggttg caggcgctcc tggccgcgct tgaaagatgc
      301 cacgtcgtag aactctcccg agacgtggat aggaacacgc tcgtcccaat tggccctgtt
      361 cgcctcccgc cactcatcgc gccattcacc catcgctctc tcctctcaga ggttctctat
      421 cggtactggc tcgaccgaat cagcgagctc gaagcccaag acccgcgagt agaaatacag
      481 ctcgccgtcc agggcccgtt tgatgttctc ggcctgcctg aagccatgtt gctcgccctc
      541 gaagggtacg taggagacag ggagcccctt ctctctgagg gcggcgaaca tcttctcggc
      601 ctgctctttg ggcaccacct catcttcgag gccctggaag aagatcaccg ggcaggagag
      661 gtgtccggtg gagtggatcg gcgagcgctc cacgtagagg tcagcccgcc cggggtaggg
      721 cccgatcagg ctgtcgaggt agcgcgactc gaacttgtgg gtctccctgg ccagggcctc
      781 cacgtcgctg actccgaagt ggctcgcgcc ggcggcgaac gtatccctga aagcgagcgc
      841 gcagagcgtc gtgtaaccgc ctgcgctacc gccagtgatc atcagccgtt cgccgtcaac
      901 caggccacgc tcggccaggt aaattgctac gttggcgcag tcgtcgacgt cgacgatacc
      961 ccacgcacct tcgaggtgac gacggtattc ccgcccgtag cccgtgctcc cgccgtagtt
     1021 cacgtcgagc acggcaatgc cgcggctggt ccagtattgg atcctcggat caagggccgt
     1081 attcgacgcc gcggtcggac ccccgtggct cataacgagg agcgggggga gctcgccatc
     1141 cggtgccgcg aaatccctgt tcttcggcgg gtagaagaaa gcgtgcgctg tccggccgtc
     1201 ctccgtagga tactcgacgg gctcggggac ggagaggtag ccagggtcta tctctaggtc
     1261 gcccgagcgt cgcaggacct cgtgcagccc ggtagaaggg tccaacctca ctatgcatgc
     1321 tgcctccgtg ggcgagctgc cgcggaagac tatgccggct gctacatcag cacgcacgta
     1381 tgcgatgctc gagtacggcg tctccaacgg ctcgagctct ccgctctccg tgtcgaggac
     1441 ggccaggtac gagctcccgc tctccgtgta cgtgcagacg atgcgtcccg gggagacgaa
     1501 ggcgtaggta gacatcccga agacccactg cggcagaccg aactccgcct ccctctcgca
     1561 gagcggctcg tcacgtccct cacgccagcg gtagaggttc caccagcccg ttttgtcaga
     1621 gacgaagtgg agcgtgcctt cgggggacca ctctggctgg aagatggatt cgtcctggcc
     1681 gccggcgatc tgctctacat tctcagggaa cccgtcggcg ccgagatcgc agcccattag
     1741 ctccgttccg tcccagggca tgttcggatg gttccacgtc agccacacga tacgccggcc
     1801 atcggggctc aggcgaggcg aggagtagaa ctcgttgccc gagacgagct cgtactcctc
     1861 tccgctttcg aggtctatcc cgactatctc gtttacgggt ccgttcccgg cgtggtcctc
     1921 ccttatggcg atcaggcgtc ggcgctcccg atccaccacc atgtccgcgt atcgtctgcc
     1981 ggtctccggc gtgagcggcg ccggctcgcc gccaggggcc cgatagtaga tcctctggtc
     2041 ggcgaagttc gagaagtaga ccatgcctcc gtcgactgta aaaccgccgc caccgtattc
     2101 gtgtacccgc gtgcgcgcgt tgaaaggctg cggcgtaacg tcctcggttc gtcggtccgg
     2161 cgtcaggagc acgacgacgt tgcgcccgcc ctcgccgggc cggctctcta gccagtacac
     2221 atccctacca tcgatcacca cttccgccag tcccactacg cccctgacga tcaagtcaga
     2281 ggagatcgga gacttccagg aaccgtaggg cgcgactcca cgttcggaca tagctcggct
     2341 cttccgcgtt acgccttggc ccttctcagt cggaggactc gaagaccagc cacgagccct
     2401 tgagaccctc acttatcgtc tcgccccccc tgcggggtac cacgctcacg agccgatcgc
     2461 cctcgcggac gaagtcctgg agcgtctcct ccacctccgt gcggtgcaga acgaagacca
     2521 cccgatggtg cttggtgagc tcctctattg actcgcgtcg cgcctcttct gcccctacgg
     2581 cttcggccag cagccgctcc cgctcggccg cttcggccca cagccggctc cggctcgcgt
     2641 cggcctcggc ctggagggcg gcctcccgct tgcgccaatc ttccaggcgc tcctcgatcc
     2701 tgtgcagtat ccgctctatg ggggacccgc ggtgctcgcc catcaccagc ctcctctatc
     2761 cgtgtgcccg agtgttactc actctacccc tgtttcgtcc gacgtgcctg atttcatact
     2821 gcctcgggac acttaggggg actacgcctt gagccggtag cctgtcttga agatccacca
     2881 caccgctgtc aggcacaaga tcaggaatag gatcgtcatg gccaggctca agcccacgct
     2941 tacgtctgcg gtcccgtaga agctccaccg gaaacagctg ataaggtaga ccacaggatt
     3001 gaagagggtg accgtctgcc agaaaggcgg caacatatct acggagtaga agctgccgcc
     3061 gagaaagatc aggggcgtaa tgataaggaa cggcacgagt tgcaacttct cgaagccctc
     3121 ggcccagatg ccgaggatga agccgaagag gctgaaggtg aaggccgtga gcacgaggaa
     3181 caagaccatc aagagcgggt gcgcgacctc cagaggcacg aacaggcccg ccgtggccag
     3241 gatgatcaag ccgagaatga ccgacttggt cgctgccgcc ccgacgta
//
LOCUS       DASTKM010000285         5978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1517880, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000285 DASTKM010000000
VERSION     DASTKM010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517880"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..873)
                     /gene="nuoB"
                     /locus_tag="VFQ10_11465"
     CDS             complement(97..873)
                     /gene="nuoB"
                     /locus_tag="VFQ10_11465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET9966004.1"
                     /translation="MGVIQQFNEPNFITTSTDKVFNWARKNSLWPLQFGLACCAIEMM
                     ATGMAHNDLDRFGAGFFPATPRQSDLMIVSGTVSTKMAERMTRLYEQMPEPKWVIAMG
                     ACAISGGPFLDGYSVLMGADRVIPVDVYVPGCPPRPEALIFGVMTLQKKIERDKQVGY
                     EKPRSALVTEEGEFLEYLPEREYTDIAEGKAERFEGIKPLKRTYVFEREGMPPAAMTD
                     VGWVPEKEHAEQGGGQEEPASPKGAADYSPKKLRESKGEG"
     gene            complement(864..1262)
                     /locus_tag="VFQ10_11470"
     CDS             complement(864..1262)
                     /locus_tag="VFQ10_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET9966005.1"
                     /translation="MALALLQGTQEISYGVLYVYVGLSILLVVGFVASVLVLARLIAP
                     SRRSSREKGRVYETGEARVTDPWHPFPVRYYVFALLFLIFDVEAAFLFPWAVIYRSLG
                     LYGFVEMVIFVLILGVGLAYAWKKGALEWV"
     gene            1639..1926
                     /locus_tag="VFQ10_11475"
     CDS             1639..1926
                     /locus_tag="VFQ10_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET9966006.1"
                     /translation="MVVREGAVVAYVITEACIGTKNTACVGACPVDCIYDGGDHYLIN
                     PAECIDCSMCLPECPVEAIYPGDEVPEEMQHYATKAAEYDYSRIAPGWGAN"
     gene            1949..3115
                     /locus_tag="VFQ10_11480"
     CDS             1949..3115
                     /locus_tag="VFQ10_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HET9966007.1"
                     /translation="MAEMKQRSLEEVKKLSVEEAVEIMRQAGIVGAGGGGFPTYFKYM
                     KPQPHLIVNATESEPGYWGDKLVQRDYLEEFLQLFEAMRAIFGLEQISMGVHEKDREW
                     FAAYQEHAADGLYDMRYVPDTYALGEEKTLIKHSTDTKVPLFLDTPDGKRRPGMPPDV
                     GIVVNNSETLLNVYNALFLAKPTTTTFFTVYGEEIDLNVYEAPIGASFAEILDIAGLD
                     VENSGHLSLLDGGPYLHDMAIEEIGNGDGYVRRMTNALFVIAKDRQGKEYADIKTPPP
                     EEGIVSLVGEVSGVHIPLGGGLLKPATPLVAEGEEVEYEQKIGEPVDEGFSIGVWASM
                     GGEISSIENDIVAIAGGAVSQEDAEAEAEASKVGGAPPRGEAEPFQEAETKTGR"
     gene            complement(3153..3986)
                     /locus_tag="VFQ10_11485"
     CDS             complement(3153..3986)
                     /locus_tag="VFQ10_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9966008.1"
                     /translation="MQGTWNLRRRLALSGGEIAYEVLGYGPPLILVHGTPSRSYIWRD
                     VALRLADRFTVYVFDLLGFGQSERAEGLDVSISGQSRVLAELVKTWELEAPSVAGHDI
                     GGAIALRTHLLEGVSFGCIALVDSVVLRPWITPATRHVKAHLDVYGTMPARVFEAIVA
                     SHLGTATYRPLDQHAFATYLEGWRGESGQRLYLLKDAQLDEEDTAEFEPLLSSIGIPV
                     SIIWGEQDAWLDPALAERLHELIPGSDLLVLPETGHFAMEDSPQVVAAALFEFFSRCN
                     E"
     gene            complement(3986..5056)
                     /locus_tag="VFQ10_11490"
     CDS             complement(3986..5056)
                     /locus_tag="VFQ10_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9966009.1"
                     /translation="MRYRSYTPGAAQSAMDFINGWFGARGLETARYTGAGPRGDQSAL
                     VVRVGAGDPRILLHGHVDVVPGEESQFEPREEGDELYGRGVYDMKGALAALMYAIEDL
                     HKLGCEATVELLVVPDEEGEHGAKTGAEVLISNGHVGDFLITGEPTDFHIGTQAKGVL
                     DVRVTLGGKSAHGSQPWLGKNAVLLAYEHYRRVADLPFTEQRSEFFPYPSVNLARIIG
                     GDVLNRVPDRCTYDLDIRFLPNQDPEEIVRQLRSVDLPADVEVLFYREPTYVSRQNPY
                     VKVLREVSARHFRGNPVGVGRHGASDVVYFQRVGVPGVEFGPVGGGHHGPNEYVVTSS
                     LETYRQMIVQFVQIVGRNGKVP"
     gene            complement(5377..>5978)
                     /locus_tag="VFQ10_11495"
     CDS             complement(5377..>5978)
                     /locus_tag="VFQ10_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9966010.1"
                     /translation="MPDRIEAGTFLMATAATGGDVTVTNALPEHLKMELEKLREMGVE
                     VIATPGSPGIRVRVEDTTSLCGVDVATLPFPGFATDLQAQMMVLLTQASGAGIITENV
                     YENRLQVAEELNRLDAGIDLFGGHRALIRGPRRLSGTIVQAPDLRGGAALVMAGLVSE
                     GTTVVDGATHILRGYEDFEEKLTTLGATVAFEAGAPIPR"
BASE COUNT         1182 a         1867 c         1861 g         1068 t
ORIGIN      
        1 ctccgcctca tccagcccgt gcctgctctg aaaaccatcg aggtagcgcc gcggcctttc
       61 ttccagttcg ccctggggca gtgttacgtc catctcttag ccctcgccct tcgactcgcg
      121 gagcttcttg ggggagtagt ctgcggctcc ctttgggctc gcaggttctt cttgtccgcc
      181 gccttgctcg gcgtgctcct tctccgggac ccagcctacg tcggtcatgg cggcgggcgg
      241 cataccctcg cgttcgaaga cataggtgcg cttcaacggc tttatgccct cgaagcgctc
      301 cgccttgccc tccgcgatgt cggtgtactc ccgctcgggg aggtactcca ggaactcgcc
      361 ttcttcggtc accagcgccg agcgcggctt ctcgtagccg acctgcttgt cgcgttcgat
      421 cttcttctgc agcgtcatga ccccgaagat gagggcctcc ggccgcggcg gacacccggg
      481 cacgtagacg tccacaggga tgaccctgtc agcccccatg agcacggagt acccgtccag
      541 gaacggacca cccgagatgg cgcatgcgcc catcgcgatg acccacttgg gctcgggcat
      601 ctgctcgtag agacgcgtca ttcgctcggc catcttggtg gagacggtgc ctgagacgat
      661 catgaggtct gactgccggg gcgtggcggg gaagaaaccg gccccgaagc ggtcgaggtc
      721 gttgtgggcc atgccggtgg ccatcatctc gatcgcgcaa cacgccaggc cgaactgcag
      781 gggccaaaga gagttcttcc tggcccagtt gaagaccttg tctgtgctcg tcgttataaa
      841 gttcggctcg ttgaattgct gtatcacacc cattcgagcg ctcccttctt ccaggcgtag
      901 gcgagtccca ctcccaaaat gagtacgaaa ataaccatct ccacaaagcc gtacaggccg
      961 agactccggt agatcacggc ccacggaaat aggaacgccg cctcgacgtc gaagatcagg
     1021 aaaaggagcg cgaagacgta gtacctgacc ggaaaagggt gccacgggtc agtaacacgc
     1081 gcctcgccgg tctcgtacac tctgcccttc tcccgcgagc tcctgcggct cggcgctatg
     1141 agccgcgcca ggaccaggac cgacgccacg aaacccacga ccagcaagat ggacaagccc
     1201 acgtacacgt agagtacgcc gtaactgatc tcctgcgtcc cttgtaacag agccaacgcc
     1261 accaaagcag ggcctctctt cgaaaagaac taagcgaaat ataaccttgc tacacgcagc
     1321 tttcaaggag ctacatgctc cttcggctgt aagatcagag cctgcgggac aaacgtagta
     1381 atgagcgccc cctccatccc gccggttcac gggatcaaaa cgtcctccct cgccgggagc
     1441 gtacgccgcc gggtgcaggc agtggtcggt gcgtcttgtt gaacgtttcc tgatcggtta
     1501 gactaatttt cgagagtctt aggaacattt gttcctaaac agctggctct tgtagggtcg
     1561 ctcttcagag gcttgctctg ggagacgtta ataacagacg ttcctacctg caaaggctaa
     1621 cggcggcgga aggtagggat ggtcgtgaga gaaggagcgg tagtggccta cgttataacc
     1681 gaagcgtgca taggcacgaa aaacaccgct tgtgtagggg cgtgcccggt ggactgcatc
     1741 tatgacggcg gggaccacta cctgataaac cccgccgagt gcatcgactg ctccatgtgc
     1801 ctgccagagt gcccggtgga ggccatctac cctggcgacg aggttcccga ggagatgcaa
     1861 cactacgcca ccaaggccgc cgagtacgac tactcgagga ttgcgccggg ctggggagcc
     1921 aactaggctc tttagggagc gatggatcat ggccgagatg aagcagcgaa gcctcgaaga
     1981 agtaaagaaa ttgagcgtcg aagaggccgt ggagatcatg cgccaggcgg ggatagtagg
     2041 cgccggcggc ggtgggttcc cgacttactt caaatacatg aagccccagc cgcacctgat
     2101 tgtcaacgcg accgagagcg agcccggcta ctggggggac aagctggtac agcgagacta
     2161 tctcgaggag ttcctccagc ttttcgaagc catgagggcg atcttcgggc tcgagcagat
     2221 ctccatgggc gttcacgaga aggacaggga gtggttcgcc gcttaccagg agcacgcagc
     2281 cgacggcctc tacgatatgc gttacgtgcc tgatacctat gccctgggcg aggagaaaac
     2341 ccttatcaaa cactccaccg atacaaaggt gcccctgttc ctggatacgc ccgacgggaa
     2401 aaggcgtcct ggaatgccac cggacgtcgg catcgtcgtc aacaactcgg agaccctgct
     2461 caacgtctac aatgcgctct ttctggccaa gccgaccacc acaacgttct tcaccgtgta
     2521 cggcgaggag atagacctga acgtctacga ggcgccgata ggggcctctt tcgcggagat
     2581 cctcgatata gccgggctag acgtcgagaa ctctggccac ctctcgctac tggacggcgg
     2641 gccttacctg cacgatatgg ctatcgagga gataggcaac ggcgacggct acgtgcggcg
     2701 aatgacgaac gccctcttcg tcatcgccaa ggacaggcaa ggcaaggagt acgccgacat
     2761 aaagacccct cctcccgagg agggcatagt ctcactggtc ggggaggtct cgggcgtgca
     2821 cattcccctg ggtggtggtc tgttgaagcc ggccaccccg ctcgtcgccg agggcgagga
     2881 agtagagtac gagcagaaga taggcgagcc ggtggacgag ggcttctcta tcggggtctg
     2941 ggcgagcatg ggaggagaga tctcctccat agagaacgac atcgtcgcca tcgccggagg
     3001 cgcagtatcc caggaggatg ccgaagccga ggccgaagcg agcaaagtcg gcggcgcgcc
     3061 gccccgaggg gaagccgaac cttttcaaga ggccgagacc aagacaggca ggtaagagac
     3121 agtgagggcg cctgcaacgg agggccagag cgctattcgt tacaccggct gaagaactcg
     3181 aagagcgccg ccgcgactac ttgcgggcta tcttccatgg cgaagtgccc tgtctccggc
     3241 aacacgagga ggtcggagcc tgggattagt tcgtgcagac gttcggcgag cgcgggatcg
     3301 agccatgcgt cttgctcgcc ccagatgatg ctgacgggaa tgccgatgga agagaggaga
     3361 ggctcgaact ctgcggtgtc ttcctcgtcg agctgggcat ctttcaggag atagagcctc
     3421 tgtccagatt ctcccctcca cccttcgagg taggtcgcga aggcgtgttg gtccagcggc
     3481 ctgtacgtcg ccgtgcccag gtgcgaggcg acgatggcct cgaagactct ggcgggcatg
     3541 gttccgtaga cgtccaggtg cgcctttacg tgcctggtcg cgggtgttat ccacggacgg
     3601 aggacgacgc tgtcgaccag ggcgatgcac ccgaaagaca cgccttcgag caggtgggtg
     3661 cgcagggcga tcgcgccccc tatatcgtgg ccggctaccg agggcgcctc gagctcccag
     3721 gtctttacca actcggcgag cactcgtgac tggcccgaga tagagacgtc tagtccttct
     3781 gcgcgctccg actggccgaa cccgagcagg tcgaagacgt agaccgtgaa cctgtccgcc
     3841 agcctcaaag ccacgtcgcg ccagatgtag gaacggctcg gcgtcccgtg gaccaggatc
     3901 aggggtggcc cgtagccgag tacctcgtat gcgatctcac ctcccgagag cgcgagacgc
     3961 ctcctgaggt tccaggttcc ctgcatcagg gtaccttgcc gttgcggccg acgatctgga
     4021 cgaactggac gatcatctgc ctgtacgtct ccagagacga ggtcaccaca tactcgttcg
     4081 gaccgtgatg tcccccgccg accggaccga actcgacacc aggcacccca accctctgga
     4141 agtagacgac gtcgctcgcg ccgtggcggc ccacgccgac agggttgccg cggaagtggc
     4201 gcgccgagac ctcgcggagc accttcacgt aggggttttg gcgcgacacg taagtgggtt
     4261 cgcggtagaa caggacctcg acatcggcag ggaggtccac cgagcgtagc tggcgcacga
     4321 tctcctcagg gtcctggtta gggaggaagc gtatgtcgag gtcgtaggtg cagcggtccg
     4381 gtacgcggtt gaggacgtcg cctccgatga tgcgcgcgag gttcacgctc ggataaggga
     4441 agaactcact cctctgttct gtgaagggca ggtcggcgac ccttcggtaa tgctcgtagg
     4501 cgaggagcac ggcgttctta cccagccagg gctgtgagcc gtgggcgctc ttacccccca
     4561 gcgtaacacg aacgtctagc acgcccttcg cctgggtacc gatatggaag tccgtcggct
     4621 cgcccgtgat caggaagtcc ccaacgtggc cgttggagat caagacctca gcgcccgttt
     4681 tcgcgccgtg ctcaccctct tcgtcgggga cgacgaggag ctcgaccgtg gcttcacacc
     4741 ccagcttgtg caggtcctcg atggcgtaca tgagggcggc gagggcgcct ttcatgtcgt
     4801 agacgccacg accgtagagc tcatctccct cctcgcgcgg ctcgaactgc gactcctcac
     4861 caggcaccac gtcaacgtgg ccgtgcagga gtatccttgg atctcctgcc cccacccgca
     4921 ccacgagagc cgactggtca ccgcggggcc cagcgcccgt atagcgcgcc gtctccagcc
     4981 cgcgcgcccc gaaccagcca ttgataaagt ccatcgcaga ctgcgccgcg cccggcgtgt
     5041 aagatctgta gcgcacgagc tggcgggtaa gcgcaaggac ctcggtattc tcggttacta
     5101 ccgccagatt ctcctccgct cttcggacgc caacaagagc agtctatatt tcagcacgcc
     5161 gccttgcagg cgaatattca gcaagtcagc aagtcgttcc gaagccacgc cacgagcgta
     5221 gtacccgacc gccgcaggca gcggtgcctc atgcgcggtc gggcggggcc ggagcggaag
     5281 aagctgaaat gctgagagcg agggccttta ggcccgaagc gtgctgacaa ggcggcggag
     5341 ccgccgtgct gaagtgccga taagcgaggg ccgattctag cgggggatcg gggctcctgc
     5401 ttcgaaggcg acggtcgcac cgagggtggt gagcttctcc tcgaagtcct cgtagccccg
     5461 aaggatgtgc gtggcgccgt ccacgacggt agtaccctcg gagaccaagc ctgccatcac
     5521 cagcgcagcg ccaccccgca ggtcgggcgc ctgcacgata gtccccgaga gccggcgcgg
     5581 tccgcggatg agcgccctgt ggccaccgaa aaggtcaatg cctgcgtcga ggcggttcaa
     5641 ctcctcggcc acctgaagcc tgttctcgta cacgttctcc gtgatgatgc cggcacccga
     5701 agcctgggtg agcaagacca tcatctgggc ctgaaggtcg gtcgcgaagc ccgggaaggg
     5761 cagtgttgcg acgtctaccc cgcagagcga cgtagtatct tcgaccctca ccctgatgcc
     5821 aggggagccc ggggtggcga ttacctccac ccccatctcc cgcagcttct cgagctccat
     5881 cttcaggtgc tcggggaggg cgttcgttac cgtgacgtcc ccgccggtcg cagccgtggc
     5941 catcaagaaa gtacccgcct cgatacggtc aggcatca
//
LOCUS       DASTKM010000286         5828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_815525, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000286 DASTKM010000000
VERSION     DASTKM010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815525"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VFQ10_11500"
     CDS             complement(<1..1631)
                     /locus_tag="VFQ10_11500"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET9966011.1"
                     /translation="MKRKALLVGLMLLLLCTLGFARGSQMDESEVLEQAGFIPTLEEY
                     YSKPTVETTADYEGSKDLWRVVLTEQTSGKEVARFKVADDSGEVSGVEVSPRADQIKY
                     PSLSEDKAIKLAAASPEVREEFARHGPHSAQAKYEDGAWTVRFYVDERGAVGGRPTEK
                     GKEIATVGVDDKTWVLDYVYTGDQVGWNLARGVRGAYGKQANYWWVWLPLALAFAAAF
                     WRTDRLLAIRNLDIVALLGFLVSHGFFREGVVLEAVVLWYPPLVYLLIRTLLMGFGIG
                     ERVEKTSDLPMWLLMVLACLAGGLVLGLNVDSRVIDVGYAGVVGADRILEGTVPYGTM
                     PSDVGTGDTYGPLNYLLYVPFVLMFGFSGEWDFLPAAHALTLFSFVAGAMALFITGYR
                     LSGKEGAAALVFAWTAFPYTVYATNNNTNDIIVAAVSAIGLAAAASPIARGASIAAGF
                     AIKLYPLVLGPLWMMYEGRKRKPIIDFVLGGAGVVLVTFWVILLDGHPIEAARLFYDR
                     TLAFQGDRISPWSLFSQVPQLAFLQRPLMALAIFLS"
     gene            complement(1730..2929)
                     /locus_tag="VFQ10_11505"
     CDS             complement(1730..2929)
                     /locus_tag="VFQ10_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HET9966012.1"
                     /translation="MRETRPRNVKEMLVEAKNTSELMVDLSYAAIFYNSEDLSEEVSH
                     LEERLNDLVFDMRTLAILAARSPADAEQMAGILQVVQDIEKIGNAAVDIAKIVVKRLG
                     IPPELLHDIPEAEEIPSRVRVHVDSALDGRSLGDVDLPVETGMRPIAIRSEDDWDFDP
                     EPEDVLRAGDVLFLQGPPEGVAEVRELAGAPPLGDAPETEPPPGELSEIDRAVDILIE
                     MKNFSEVAVGLAYSALLYGDAGLAREVVAIEDEMDEMRYRLERWVLLAAPRVEDSSRL
                     RGLLHLATASETIADCAMEMVWIVEKGEEVHPVLSAAIGESDEIVLKLTVSPGSPADG
                     KTLGSLKIETETGMYALAVNRGGRWTYRPRDTYRLQGGDSLLVTGAHEGLEPLAELFG
                     QELEDSP"
     gene            complement(2933..4183)
                     /locus_tag="VFQ10_11510"
     CDS             complement(2933..4183)
                     /locus_tag="VFQ10_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET9966013.1"
                     /translation="MRERLRDRPRLRPKVYGYLTEEKETLGQGFAALFLSSAGELIAG
                     IMLAGISGTLDELVGLAVLIPAAIGMRGAIFGAMGSRLSTSIQTGLFRFDLRRGVLAE
                     NVQAAAVLSLVSGVFLAFLARILCGILGVRTELSVIDYVIISTAGGIIAGVLLLGITV
                     FVARLTVARGWDMDNIAAPMLTAAGDILTLPSLVVATYLIGIPVFSLVLGVALVLAAG
                     VAAYLGLRVGVENLRRIVAESFPILAMTGTVTILVGVALQDREEQFTTLLALSILLPA
                     FLQEGGALGGILSSRLSSKIHLGLIAPRGVPQFAALRDFTLTYIFAAGVYVFIGGASH
                     LIAVALFEEGASPGFLAMIGVSALAGLIATTAAVLAAYYGSTLSYRLGLDPDTYGIPI
                     ITAAVDLLGFMSLIIALIVFGLAG"
     gene            4414..5364
                     /locus_tag="VFQ10_11515"
     CDS             4414..5364
                     /locus_tag="VFQ10_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9966014.1"
                     /translation="MSKIKAVVVDPGAPNRLALADVEEPSPTLSEALVRVAAVSLNRG
                     EVRRAESSEPGFRPGWDLAGTVERAAADESGPPEGSRVVGFLPSEAWAELVAVPTDSV
                     AALPEGVSFEEASTLPVAGLTALYALEEGGNLLGRSVLVTGASGGAGQFGLQLARAAG
                     ARVVGLVRREEHAGLVEEIGAHEVVVDESGAAARERGPYDLVLESVGGEVLGNALSML
                     VPGGTCVSFGASASAEVEFDTRTFYLTGGARLYGFILFHEVRARPASGGLGRLARMVA
                     DGRLKPRIEVQAPWTEVGEIARRLIERGYTGKAVLRVGPN"
     gene            complement(5377..>5828)
                     /locus_tag="VFQ10_11520"
     CDS             complement(5377..>5828)
                     /locus_tag="VFQ10_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9966015.1"
                     /translation="LLALETGPATPKVDVRAAVFREERILLVKEPGEGGWSLPGGWAD
                     VGESPSEAAVRETLEESGYRVRPVRLLAAYDRDRQGHPPIPYHVYKLVFLCEILDEVP
                     SSNVDTDEVRFFGDNELPELSITRVTPAQVSRFFEQHRDPGLPTYFD"
BASE COUNT         1058 a         1831 c         1967 g          972 t
ORIGIN      
        1 gagaggaaga tggcgagcgc catcaacggt cgttgcagaa acgcgagctg agggacctgg
       61 ctgaacaggg accacgggct gatcctgtcg ccttggaagg cgagggtcct atcgtagaag
      121 agcctcgccg cctcgatggg atgcccgtcg agcaggatga cccagaacgt gaccagtacg
      181 accccggccc cgccgagtac gaaatctatg atgggcttgc gcttgcgacc ttcgtacatc
      241 atccacaagg gtcccaggac gagcggatag agcttgatgg cgaagccggc cgcgatgctc
      301 gccccccgag caatcggcga ggccgccgcc gcgagcccta tcgccgagac ggcagcgacg
      361 atgatgtcgt tggtattgtt gttggtagcg taaacggtgt aggggaaggc tgtccaggcg
      421 aagacgaggg cggcagcccc ttccttgccc gagagcctgt agcccgtgat gaaaagcgcc
      481 atcgcccccg cgacgaagga gaacagagtg agggcgtggg ctgcggggag gaagtcccac
      541 tctccggaga acccgaacat gaggacgaag ggcacataga gcaggtagtt cagagggccg
      601 taggtgtctc ctgttcccac gtctgagggc atggtgccgt agggtactgt gccttccagg
      661 atacggtcgg cgccgacgac gccggcgtag cctacgtcta tgacgcggga gtcgacgttg
      721 aggccgagga cgaggccacc ggcaagacag gcgagcacca tcaggagcca catcgggagg
      781 tcgctcgtct tctcgaccct ctcgcctata ccgaaaccca tgagaagagt cctgatgagg
      841 aggtagacga gcggcgggta ccacaggaca acggcttcca ggactacccc ctccctgaag
      901 aagccgtgcg agacgaggaa cccgagtagc gccacgatat ccaggtttcg tatggctaac
      961 agcctgtccg tgcgccagaa agcagcggcg aaggctagcg ccagtggtaa ccagacccac
     1021 cagtagttgg cctgcttgcc gtaggcgccg cgtacgccgc gggccaggtt ccaccccact
     1081 tgatcccccg tgtaaacgta gtcgaggacc caggtcttgt cgtccacccc gacggtggcg
     1141 atctccttgc ccttctccgt cggcctgccg ccgacggccc ccctctcgtc gacgtagaag
     1201 cggaccgtcc acgcgccatc ctcgtacttg gcttgtgcac tgtgaggtcc gtgccttgcg
     1261 aactcctccc tgacctcggg gctcgctgcg gcgagcttga tcgccttatc ttccgagagg
     1321 ctggggtatt tgatctgatc cgctctcggc gagacctcga ccccgctcac ctctccggaa
     1381 tcgtcggcga ccttgaaacg ggcgacctcc ttccctgaag tctgctccgt aagtacaacc
     1441 cgccagaggt ccttgctccc ttcgtagtcc gcagtagtct cgacagtcgg tttggaatag
     1501 tattcctcca gcgtcgggat aaagccggcc tgctccagca cctccgactc gtccatctgg
     1561 gagccccgcg cgaagcccag agtacacagc agcaacagca tgagccccac cagcaatgcc
     1621 ttacgcttca cgagggtctc tccgtcgctt gcggggcgag caggcgcatg cctgttgccg
     1681 gcggcgccag gccccggacg gtgcggtact tctggggagc cttcccggat tacggggaat
     1741 cctccagctc ctggccgaag agctcggcga gaggttcgag gccttcatgg gcgcccgtaa
     1801 cgagcagcga gtcgccgccc tgcagcctgt aggtgtcgcg gggcctgtag gtccagcgtc
     1861 cgccccggtt cactgcgagg gcgtacatgc cggtctcggt ctcgatcttg agggagccaa
     1921 gcgtcttgcc gtcggccggc gaccctgggg agacggtgag cttcagaacg atttcgtccg
     1981 actcgccgat ggccgcggag agtacaggat ggacctcctc gcctttctcg acgatccaga
     2041 ccatctccat ggcgcagtct gcgatggtct ccgaggcggt ggcgaggtga aggaggcccc
     2101 tgaggcgcga ggagtcctcc acacggggtg cggcgagaag gacccagcgc tccagccggt
     2161 agcgcatctc gtccatctcg tcctcgatgg cgaccacctc gcgggcgagc cccgcgtcgc
     2221 cgtagagcag ggccgagtag gcgagcccga cggccacctc tgagaagttc ttcatctcta
     2281 tcaagatgtc caccgcgcgg tcgatctccg agagttcgcc tgggggcggc tcggtctcag
     2341 gggcgtctcc gagcggaggg gcgccggcga gctccctgac ctcggcgaca ccctctggcg
     2401 ggccttgcaa gaagaggaca tcgccggccc tcagtacatc ctcgggttcg gggtcgaagt
     2461 cccagtcgtc ctcggagcgg atggcgatag gccgcatccc cgtctccacg ggcaggtcga
     2521 cgtcgcccag agagcggccg tccagggcgc tgtcaacgtg caccctgacg cgggagggta
     2581 tctcttcggc ctctgggatg tcatgcaaga gctcgggcgg gatcccaaga cgcttgacta
     2641 cgatcttggc tatgtcgacg gcggcgttgc cgatcttctc tatgtcctgg acgacctgga
     2701 ggattcccgc catctgttcc gcgtcggcgg gactcctcgc ggccaggatg gccagggtgc
     2761 gcatgtcgaa gaccaggtcg ttgagacgct cctccaggtg ggacacctct tccgagaggt
     2821 cttcggagtt gtagaagatc gccgcgtagg agaggtcgac cataagctcc gaggtgttct
     2881 tcgcctcgac gagcatctcc ttcacgttcc gtggtcgcgt ctcgcgcatc tcttaccccg
     2941 ccaatccgaa aacgataagg gctattataa ggctcataaa gccgagcagg tccactgccg
     3001 ccgtgataat cgggatgccg taagtgtctg ggtccaaccc gagcctgtag ctgagggtcg
     3061 agccgtaata tgctgcgagt accgccgccg tggtggcgat caggccagca agggcgctga
     3121 ccccgatcat tgccagaaag ccaggactag ccccctcttc aaagagcgcc acggctatga
     3181 ggtgcgaggc tcccccgatg aacacgtaga ctccggcggc gaagatgtag gtgagcgtga
     3241 agtctcgcaa ggccgcgaac tgcggtaccc cgcgcggcgc tatgaggccg aggtggatct
     3301 tggaggagag gcggctggag aggatgccac ccagggcgcc gccctcctgc aagaacgcgg
     3361 gcagaagtat gctcagggca aggagcgtag tgaactgctc ctcccgatcc tgcaaagcca
     3421 cgccgactag gatggtcacg gtccccgtca tggccaggat ggggaacgat tcggccacga
     3481 tgcgcctcag gttctcgacg cctactctca ggcccaggta cgccgccacg cccgccgcca
     3541 ggaccagcgc aacgccgagt accagggaga agacagggat gccgatgaga taggtggcca
     3601 ctacaagaga ggggagcgtt agtatgtcgc cggccgcggt gagcatcggg gctgcgatgt
     3661 tatccatatc ccagccgcgg gcgaccgtga ggcgggcgac gaagaccgtg atcccaagca
     3721 gcagcacgcc agcgatgatc ccgcccgcgg tcgaaatgat gacgtaatcg atgaccgaga
     3781 gctcggtcct gacgccgagg atgccgcaca ggattcgcgc caggaaagcg aggaacacac
     3841 cggagacgag cgagaggacg gccgcggcct gtacgttctc ggcgagcacg ccgcgcctga
     3901 ggtcgaacct gaacaggccg gtctggatgg aggtcgagag cctgcttccc atagccccga
     3961 aaatggcgcc gcgcatccct atggccgcgg ggatcagaac tgcgagaccg acgagctcgt
     4021 cgagcgtgcc tgagatgcct gcgagcatga tgccggcgat aagctccccc gccgaggaga
     4081 gaaacagcgc cgcgaaaccc tgcccgaggg tctccttctc ctctgtgaga tagccgtaga
     4141 ccttcgggcg caggcggggc cggtcgcgca gcctctcgcg caccccaccc tcccggccgc
     4201 cctctcgcca ccggacccgc cttttctctc tctcggacac ggggaatgta tagtctctgc
     4261 agcccgcgaa gtaaagcctc cccttaggag aaggtgactg caaacagcag cctggctcac
     4321 cgatctatag catacctgga gcggcctgat cggtgcagtg ggaagccggt ctcgcttcgc
     4381 aaggcgcggc caacgaacgg aaagggagca cctatgagca agatcaaggc tgtagtcgtc
     4441 gaccccggcg ccccgaaccg cctcgcgctg gcggacgtgg aggagccgtc tccaacgcta
     4501 tcggaggccc tggtgcgcgt ggcggccgtc tcgctcaacc ggggcgaggt gcgccgggcc
     4561 gagtcgagcg agccagggtt ccgacccggc tgggatctgg caggcaccgt cgaacgcgct
     4621 gccgcggacg aatcgggccc gccggagggc tctagggtag ttggcttcct cccttccgaa
     4681 gcgtgggccg agctcgttgc cgtgccgacc gactcggtgg ccgcgctgcc agagggcgtt
     4741 tcgttcgagg aagcttcgac gttgccagtc gctggcctca cggccctata cgcgctggag
     4801 gagggcggca acctgctcgg gaggtccgtg ctggtaacgg gcgcctctgg cggggccggg
     4861 cagttcgggt tgcagctcgc ccgagcggcc ggggcgcggg tggtcgggct cgtgcgccgg
     4921 gaggagcacg cgggcttggt ggaggagatc ggcgcccacg aggtcgtcgt ggacgagagc
     4981 ggtgcggcgg ccagggagcg gggtccttac gatctggtcc tcgaatcggt cggcggggag
     5041 gtgcttggga acgcgctctc gatgctggtg ccgggcggga cctgcgtaag cttcggggca
     5101 tcagcttcgg cggaggtaga gttcgacacc aggacgttct acctgacggg cggggcgagg
     5161 ctctacggct tcatcctgtt ccacgaagtg cgggcgcggc cggcctcggg tggtctcggc
     5221 aggctggcgc gaatggttgc ggatggaagg ctgaaaccgc gcatcgaggt gcaggcgccc
     5281 tggacggagg tgggggagat cgccaggcgg ctcatagagc ggggttacac cgggaaggca
     5341 gtcctccggg ttggcccgaa ctagacggag ccgcccctag tcgaagtagg tgggaaggcc
     5401 agggtcgcgg tgctgctcga agaagcgtga gacctgggct ggggtaacgc gggtgatcga
     5461 gagctcagga agctcattgt caccgaagaa gcgcacttcg tcggtatcca cgttggatga
     5521 aggcacctcg tccaagatct cgcacaagaa cacaagcttg tagacgtggt aggggatggg
     5581 cgggtggccc tgcctgtcgc ggtcgtaggc tgcaagcaac ctgacgggcc gcaccctgta
     5641 gcccgactct tccagggtct cgcggaccgc ggcttctgac ggagattcac ccacgtccgc
     5701 ccagcctcct ggcaaggacc aaccaccttc gcccggttcc ttgacgagca ggatccgctc
     5761 ttccctgaac accgcggccc ttacgtccac cttcggcgtc gcaggaccgg tttcgagagc
     5821 gagcagat
//
LOCUS       DASTKM010000287         6181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1344444, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000287 DASTKM010000000
VERSION     DASTKM010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344444"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..975
                     /locus_tag="VFQ10_11525"
     CDS             370..975
                     /locus_tag="VFQ10_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9966016.1"
                     /translation="MRERTNAQWLAELRGPNPNEALADLYDLLVRGLRAALGGRAGAN
                     IEDFAQEALLKITNNLDSFRGESHFTTWAKKIAMNVALTELKRRRWRDVSLQELLAGH
                     QGTGRGLADLQLTPEQVAFQNIVLAEVRGIIDKELTDRQREAVVAVILEGMPIAEVAR
                     RMGTNQNALYKLLHDARKKLKLRMEAVGLPPQEVLAAFDTG"
     gene            1030..1257
                     /locus_tag="VFQ10_11530"
     CDS             1030..1257
                     /locus_tag="VFQ10_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966017.1"
                     /translation="MLDRGDLDKLMRALAETREREIGCDECFEQMDRFAEIEFSGSDS
                     ATAMPLVHDHLRKCEDCHEKYEALLIAIGAG"
     gene            1489..2334
                     /locus_tag="VFQ10_11535"
     CDS             1489..2334
                     /locus_tag="VFQ10_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HET9966018.1"
                     /translation="MANSEAPEPLARYLAQIGRERLLTHEEEIDLSRRVRAGDKQARE
                     DLIEKNLRLVVSVAKKYRGMGLPFGDLIQEGNIGLMKAVEKFDPDRGYRFSTYATWWI
                     RQAVQRAVADKGRIIRVPVHMGEKMRKMARVYNELSAELGREPTDQEVARKLGWDVDR
                     VKNVKSAIPDATSLNRTLSSDEDSSELGDFIEDEREASIAGQVVRELETHRLMESVER
                     VPERQRRVLVRRYGLDGKEPATLADLSEELGVSRERVRQLQREAEGVLRDAAGEHRLG
                     FPTAA"
     gene            2544..3191
                     /gene="msrA"
                     /locus_tag="VFQ10_11540"
     CDS             2544..3191
                     /gene="msrA"
                     /locus_tag="VFQ10_11540"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET9966019.1"
                     /translation="MLFSRHKTKMIAPAKALPGREQAAFELPERHAVLGTPLEPPFPE
                     GLSTAVFGLGCFWGAERKFWQIDGVYTTAVGYAGGFTPNPTYGEVCSGRTGHSEVVLV
                     VFDPNKVSYRDLLRVFWEAHDPTQGMRQGNDVGTQYRSAIYYSDNEQRRAAEETREAY
                     EEALRRGGYGPITTEIAPTGPFYYAEGYHQQYLHKVPNGYCGLGGTGVSCPVGID"
     gene            3381..3515
                     /locus_tag="VFQ10_11545"
     CDS             3381..3515
                     /locus_tag="VFQ10_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966020.1"
                     /translation="MTDNTVDAAAPDTIVLIHGFWMTPRRRTQHAPQPEHPPVDAGGD
                     "
     gene            complement(3638..4246)
                     /locus_tag="VFQ10_11550"
     CDS             complement(3638..4246)
                     /locus_tag="VFQ10_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET9966021.1"
                     /translation="MRYVVLILLTVFLASGLVVLVDTRTEPVLAASALRGPDQILGAP
                     RYSQATVERYARSIDCSRYIMKTIPIYYRLAPRRNIAPDVLVAQSIVETGRGYYGGVS
                     KPWNMAGIKKGGEDRGDAPKDFEVPPTAYEGVKMHVNHMAAYTGKKPIGKPHDRFYDA
                     RAAQKQRGWWVRRISQLGGGIWAVGPTYASKIRDHLDNMGRR"
     gene            4461..4598
                     /locus_tag="VFQ10_11555"
     CDS             4461..4598
                     /locus_tag="VFQ10_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET9966022.1"
                     /translation="MLAGADLRECHFYGAVMRRPDLSRTWLEEADFSGANHAILEDTL
                     R"
     gene            4630..5013
                     /locus_tag="VFQ10_11560"
     CDS             4630..5013
                     /locus_tag="VFQ10_11560"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HET9966023.1"
                     /translation="MQAGGTSLTRSEVSEIREKGFGQFGETKRGNFVIVDFNFTNNSS
                     EDVTLDSASVSLIDSSRNKSEVDPEYSSYVPAKKDIFLENVNPDVARPGEVIFTVASG
                     ASGFKLQVADTNAFSGKNGNVDLGF"
     gene            5265..5567
                     /locus_tag="VFQ10_11565"
     CDS             5265..5567
                     /locus_tag="VFQ10_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966024.1"
                     /translation="MNEWGEIRAVLSNDVIIPRLCVSVRVTCVGAGNNGHEHHGYDGN
                     HHRHHHRYDNGYHGHHHRHEHHWHYRHHHGYDGNHYRYDNGYHGNHHKHPTKHPAT"
     gene            5599..>6181
                     /locus_tag="VFQ10_11570"
     CDS             5599..>6181
                     /locus_tag="VFQ10_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966025.1"
                     /translation="MDKGPLAGGTAIGKDVLVPGDVIDILPDGTRVNGIDQIIIETEN
                     CELRAQGNDLTITMSDRGVPFRIRDGDNADITLESDGTIVANGRSTLGESFPEAVRDN
                     PDKLIVPIPVDPENDVFPRGPNDTFPIISSTGVGGDGCRVVGDSRDGGGDAGNVDGDA
                     SIDKGNINAGDVNSKDGVIPDTISEHQVPNTGGM"
BASE COUNT         1417 a         1704 c         1924 g         1136 t
ORIGIN      
        1 gccgacagtg gccgcatcta cggctggtcg gtagacgcgg acggtgcgct cgagcccatc
       61 gggtcgtggg agggcgtgcc gaagacggtg gccgggctcg cggcgagctg acgccggagc
      121 gagatgcgac tggagactct ctagcatctg gtttgcatcg taaccgacaa ctcgtaatct
      181 acggactcct gagaccactc tgataccacc gcgagcggaa tacggtacac gatgaggaaa
      241 ggaatcattt tgcaggagaa atgtagcgtg accgaggtga gtccggcggc aagacagtac
      301 cgttggcaga agtagagcag tctctgcaat aacacggtgg acgaggatgc gggatagtat
      361 cagattcaga tgcgagaacg tacaaacgca cagtggctcg ccgagttgcg gggacccaac
      421 ccgaacgagg cgcttgcaga cctctacgat ctgcttgtgc gggggttgag agcagctctg
      481 ggcggtcgtg ctggtgcgaa catcgaagac ttcgcccaag aggcattgct caagataacc
      541 aacaacctgg actctttccg gggggagagt catttcacca cctgggcgaa gaagatcgcc
      601 atgaacgtgg ccctcaccga gctcaagcgc aggcgctggc gcgacgtttc tttgcaggaa
      661 ctgttggccg gacaccaagg gacaggccgt gggttggcgg atcttcagct gacccccgaa
      721 caggtggcgt tccagaacat agtgctcgct gaggtgcgcg gcatcataga caaagaactg
      781 acggatagac agcgcgaggc tgtagtagcg gtgatactgg aggggatgcc catagccgag
      841 gtagcgaggc gtatgggcac caatcagaat gctctctaca agctgctgca cgacgcccgc
      901 aagaagctga agctgcgcat ggaggccgtg ggactccctc cacaggaggt gctggcagcc
      961 ttcgacacag ggtaagacct cgagcgagta gcgagtctag gacatcagaa gtggaacagg
     1021 agatttgcaa tgttggatcg aggtgacctc gacaaactga tgcgagccct ggccgagacg
     1081 cgcgagcgtg agatcggttg cgacgagtgc tttgagcaga tggaccgttt cgcagagata
     1141 gagttctccg gatcggattc tgcaacggcg atgccgctgg tacatgatca tttgcgtaag
     1201 tgcgaggact gccacgagaa gtacgaggcc ctcctgatcg cgatcggtgc gggttagggc
     1261 gcgagccgaa tcggggatct aggtctggag cgctagggcg gaaaccagaa cgagtccatg
     1321 aaagggtaag aaatagggcc tccagatggt ctctatatac gagagcccag taagaaggag
     1381 acggcaccaa ggagactcga cgagaagatt tcctgtagac cgcagagtcc gatgacgaca
     1441 aagggttttg tgaggaggta caagatagca agagagggag taacgagaat ggcgaatagc
     1501 gaggctccgg agccgctggc gagatatctg gcgcagattg gccgggaaag gctactcacg
     1561 cacgaggagg agatagatct cagtaggagg gtgagggctg gtgacaagca ggcgcgagag
     1621 gatctcatcg agaagaatct gaggctggtg gtgtcggttg ccaagaagta ccgtggtatg
     1681 gggctgccgt tcggagatct catccaggaa ggcaatatag ggctgatgaa ggcggtggag
     1741 aagttcgatc ctgacagggg ataccgcttc tccacctacg ccacatggtg gataaggcag
     1801 gctgtacaga gggctgttgc agacaaggga aggatcatcc gagtgcccgt gcacatgggc
     1861 gagaagatgc gcaagatggc ccgagtctac aacgaactct ccgccgagct cgggcgcgag
     1921 cccaccgacc aggaggtcgc caggaagctc gggtgggacg tggaccgggt caagaacgtg
     1981 aagagtgcca tccccgacgc caccagcctc aaccgaactc tctcctcaga tgaggatagc
     2041 tcggaactcg gtgattttat tgaggacgag cgggaggcca gcatagccgg ccaggtggtg
     2101 cgggagctcg agacgcaccg cctcatggag tctgtcgaga gagttcctga gcggcagcgc
     2161 cgcgtcctcg tccgccgtta cggcctggac ggcaaggagc cggcgacgct cgccgacctc
     2221 agcgaggaac tcggcgtctc gcgtgagcgc gtaaggcaat tgcagcgcga ggcggaggga
     2281 gtgctcagag atgcggcagg agaacatcgc ctggggttcc cgaccgccgc ttaggactct
     2341 atgacgcgtg cactctctgc gcagcggaaa tgtggccgac cagtcgctcg cctatcgggt
     2401 cgtaagctag gaggcaaact tcaccgaact tgggattagt gtgtacacct atctggggtt
     2461 tgctcgaggg tgataaacca agacgagaca agcttccgaa agattttggt ctagcaaagt
     2521 gttgtgttga aggagggaag aaaatgctct tcagtcgcca taagaccaag atgatcgccc
     2581 ccgccaaggc gcttcctggc cgggagcagg cggcgttcga acttccggag aggcacgccg
     2641 tccttggcac gcccctcgag ccacctttcc ccgagggtct gagtaccgcg gtcttcggcc
     2701 tcggctgctt ctggggcgcc gagcgcaagt tctggcagat cgatggtgtc tatacgaccg
     2761 ccgtcggtta cgcaggaggc ttcacaccaa acccgaccta tggggaggtg tgcagcggcc
     2821 gaaccggaca ctctgaagtc gtgctggtcg tcttcgaccc caacaaggtc tcctaccgag
     2881 acttgctgag agtattctgg gaggcccacg acccgacgca ggggatgcgc cagggcaacg
     2941 acgtaggcac gcagtaccgt tcggcgatct actactccga caacgagcag cgccgggctg
     3001 ccgaagagac ccgcgaggcc tacgaggagg cactgaggcg tggcggctac ggtccgatca
     3061 ccacggagat cgcacccacc ggaccgttct attacgccga gggctaccac cagcagtatc
     3121 tgcacaaagt accgaacggg tactgcggtt tgggaggaac cggcgtgagc tgcccagtcg
     3181 gaatcgacta gatgggcgca cagaatgatc gaacagtcaa tggctctcct gcggcaaact
     3241 ggtagaggat atcgaccagt ctctgtccaa ttatcatatg cacctatcta gcacacaagg
     3301 atacgccaaa tggggatacg ccgggcttct ccgctggagc acaatttgag tcgaggaaag
     3361 agcaacgtca ggagaaccgc gtgaccgaca acaccgtcga tgcggccgca ccagacacca
     3421 tagtgctgat ccacggcttc tggatgaccc cacgcaggcg aacgcaacac gccccgcagc
     3481 ctgagcaccc gcccgtcgac gcgggtggcg actgaaggcg gagactccta cgagcagtgg
     3541 gttacttccc tggagctgtt cttcgatctt gtcttcgtct tcgccctcac ccagttagcg
     3601 aggctcccgc tcacaaccat gctctgtatc aatctagcta gcgtctaccc atattgtcga
     3661 ggtggtcccg gatcttgctc gcgtacgtgg ggcctacggc ccaaatcccg cctcctagct
     3721 ggctgattct cctgacccac cacccacgct gcttctgggc tgcgcgcgcg tcgtagaagc
     3781 ggtcgtgcgg cttgccgatg ggtttcttgc ccgtgtaggc tgccatatgg ttgacgtgca
     3841 tcttgacccc ttcgtaggcg gtcggaggca cctcgaagtc ctttggagcg tcacctcgat
     3901 cctcgccgcc cttcttgatg ccggccatgt tccagggttt ggagactcct ccatagtatc
     3961 ctctcccggt ctcgacgatg gactgggcta ccagcacgtc aggcgctatg ttcctcctcg
     4021 gagctagcct gtagtagatc ggaatagtct tcatgatata gcggctgcag tcgatagagc
     4081 gcgcatacct ctctactgtg gcctggctgt agcgaggagc gccaaggatc tggtcgggtc
     4141 cgcggagtgc gctcgctgca agtaccggct cggtccgcgt atcgacaagc accaccaggc
     4201 ctgaagcaag gaacaccgtc aacaagatca aaacgacgta ccgcaccaca cccctcctgc
     4261 aactggagac cttggctctt cgttctcagt tcgacgcact atatagcatt cgtcagccaa
     4321 ggtatacaca ctcggcattg cagccctgaa tcacgccacc tggcagatgg accgtgtctt
     4381 ggatgtgtcc atgctttgct ccattgactg acttgtgtag gccaagccac gaagtattca
     4441 cgcaagtagt agaggagacc atgctcgcgg gcgccgacct gcgcgagtgt catttctacg
     4501 gtgccgtgat gcggcggccg gatctctcgc ggacgtggct cgaggaggcc gacttctccg
     4561 gcgccaatca tgcaatcctg gaggacaccc tgcgttgagt ctcccgaacg aacatgttcg
     4621 aggaaagtat tgcaggccgg cggaacgagc ctcacccgca gtgaagtgag cgagatcaga
     4681 gagaagggtt tcggccagtt tggcgagacg aagaggggca acttcgtgat cgtggacttc
     4741 aacttcacga acaactcaag cgaggacgtt acactcgatt cggcgtctgt ctccctcatc
     4801 gacagcagta ggaacaaaag cgaggttgat cccgaatact ctagctacgt cccagccaag
     4861 aaggacatat tcctggagaa cgttaacccc gatgtagcgc gaccgggaga ggtgatcttc
     4921 accgtcgcct ccggtgcgtc aggtttcaag ctgcaggtgg cagacacgaa cgcgttctcg
     4981 ggcaagaacg gtaacgtcga cttaggcttc tgagccttgt tggaacgagc cgtgggacag
     5041 aagatctact cgtttgcgga tcaatctcca tggcatgcgc taaggtagag gacgttgtaa
     5101 aagtggtcgc cgcgtcctct caaagaacct cactacactg catccgactt caccccgttc
     5161 gttcagttca ttcaatggcc tgtcgtagat atggctatgg tatacctgcc ttacaagttg
     5221 catgatttgc gccgatgcac taatgtgaag caggtaatat gtagttgaac gagtggggag
     5281 agatacgtgc cgtactgtcg aacgatgtta tcatccctcg cctttgtgtt agtgttcgcg
     5341 ttacctgcgt gggggcaggc aacaacgggc acgagcacca cgggtacgac gggaaccacc
     5401 acaggcacca ccacaggtac gacaacgggt accacgggca ccaccaccgg cacgagcacc
     5461 actggcacta caggcaccac cacgggtacg acgggaacca ctacaggtac gacaacgggt
     5521 accacgggaa ccaccacaag caccccacca agcatcccgc cacctgagga ctcatcgccg
     5581 cagcccgagc cgcaggatgt ggacaaagga cccctggcgg gaggaacggc tatcggcaag
     5641 gatgtgctcg ttcctggaga tgtcatagac atactccctg atggcaccag ggtcaacggg
     5701 atagaccaga tcatcataga gaccgaaaac tgtgagctta gggcgcaggg caatgacttg
     5761 accataacca tgagcgatcg tggcgtgcct tttcgtatca gggacggtga taacgcagac
     5821 atcacgttgg agtcggacgg aaccatagtc gccaacggca ggagtacgct tggggaatcg
     5881 ttccctgagg cagtgagaga caacccagac aagctcatcg taccgatacc ggtggatccg
     5941 gagaacgacg tgtttcccag gggacccaac gacaccttcc cgatcataag ctcgacgggc
     6001 gtgggtggag atggatgtcg tgttgtgggt gacagcagag atggcggcgg agatgccggc
     6061 aacgtcgacg gtgatgccag catagataag ggcaacatta atgctggcga cgtgaactcc
     6121 aaagacggtg tgatccccga cacgatctcc gaacatcagg tgcccaacac gggcggtatg
     6181 c
//
LOCUS       DASTKM010000288         7801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1520593, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000288 DASTKM010000000
VERSION     DASTKM010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520593"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFQ10_11575"
     CDS             <1..464
                     /locus_tag="VFQ10_11575"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HET9966026.1"
                     /translation="LGAGKDATLRDAMRFSAARHALAREYARDFEVTRELARPALLSS
                     LSRADSVRGALVQAYLEVLSEVPDLDVAGRAGRQEAEEVSRMAHGVLKAGGIHSRRGL
                     EGIANLGGLLRADSRLSPTTTEYPVIAAAFLVAMEYGPDALSHRLRPATGR"
     gene            533..1288
                     /locus_tag="VFQ10_11580"
     CDS             533..1288
                     /locus_tag="VFQ10_11580"
                     /inference="COORDINATES: protein motif:HMM:NF002671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HET9966027.1"
                     /translation="MRALVIVGHGSHLNGDSSLPVYEHAGRIRETGEFDEVVECFWKE
                     EPSMRHVLDTIESEDVYIVPAFISEGYFTQQVIPRELGLEGPVSTKGEKTVRYAGPLG
                     TFEGMPDVILERTQDLMRGKEVSGRTALILLGHGTELNKNSGGVIYLNAGRIRERGIY
                     DLVEVGFLDQDPEIGGVVDGVEAQNVILIPLFIAEGWHTRETIPEDLGLVGEVTVRDD
                     KTIFYGAPVGTHPSMANLIAARARGMMTEQPVV"
     gene            1281..2108
                     /locus_tag="VFQ10_11585"
     CDS             1281..2108
                     /locus_tag="VFQ10_11585"
                     /inference="COORDINATES: protein motif:HMM:NF037718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DR2241 family protein"
                     /protein_id="HET9966028.1"
                     /translation="MSDARSGCPENVAASSRQAFAAWVDDAPEGRVFGQVVVRPVPPL
                     GYSLRHVDEAREEGLSVSDDPHDAREIARLTEGGEYRPLKSSPNLRRGWELRVEDALS
                     LVTAMNYLYPAGVVHWYLHREGRLHLTSFREAAARQSGIYKRVQRLTDQGVLDAALAC
                     CKDSVCLKKTLWDVDEDTPLDVDRGEGEIPCPEPCSIFISFARRVRLFEREKDLDRTG
                     LSPSETEDLAALVEAAATGEVKFAREAEFEKPLNERRMRYRRLTLVPKLRRNGQSAS"
     gene            2215..2904
                     /locus_tag="VFQ10_11590"
     CDS             2215..2904
                     /locus_tag="VFQ10_11590"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9966029.1"
                     /translation="MARKKRRAARAGAKAGARVAPVAGRTVGKAAWKLGKAEAKLIRR
                     AMSSQEPKKTRYLKYGFFILIGLAIGAAIARSKKGESSDESSSYTGTTGPHAPDPGSP
                     AGERGQTWGSGTPTGTAGGGGQQSPGGANRTGADREYSDPSSGPLIGEQHGNVEGVGE
                     QQEEIEQRIRTHLGEDPRTAEMPRVNVEVNDGIAELRGAAPSEDAKLAAGEIAQQVEG
                     VREVRNLIQVS"
     gene            complement(3091..4446)
                     /locus_tag="VFQ10_11595"
     CDS             complement(3091..4446)
                     /locus_tag="VFQ10_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET9966030.1"
                     /translation="MLDESIFKAYDIRGVYPESLDEGVATDIGRAFVKHLDLSGSRVV
                     VARDMRISGEALEKAFIQGVTQAGADVLDLGLVSTDALYFAVGHLEEPGGAMVTASHN
                     PKDYNGFKLCREEAIALSGDAGLAQIRDLIVSGDLPGPAEYPGSVEETDITVDYAKHC
                     LSFIQTEGLRPLKIVIDAGNGMAGKMVPPIFEHLPFEYVPMYFELDGSFPNHPPNPIE
                     PENVRELQERVVAEGADFGVAFDGDADRCFIIDENGTTISGDLAAALIAKNVLEKKPG
                     ATILYSAVCSKAFPELVEREGGKAIRTRAGHSIIKPQMRKHDAAFGGEHSAHFYFRDN
                     YFADSGIIAMLTIAELVGRQEEPISRLLSPIDPYVRSGEINSEVEDQGATLEKIEDHF
                     SNNDGAKVDHLDGLTVDLGDWWFNLRPSNTEPLLRLNVEAEDNETMVRKRDELLNLVR
                     D"
     gene            4566..5120
                     /locus_tag="VFQ10_11600"
     CDS             4566..5120
                     /locus_tag="VFQ10_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9966031.1"
                     /translation="MSPRFVRFAALFYGSLAVVAALWCGLRGFNVRLLGESPGASVLL
                     GVITAACTVSLGLLAYRLLPTLREISDELAPRLVDGAASSSLVLVAIFSGVGEEAFFR
                     GALQQEFGLVVASLLFGLAHIGPDRRYLVWTAWAVLVGFVFGFLYEVSGGLLAPMLAH
                     TAHNAATLLLWKRSRKDPKKAGGL"
     gene            5177..5680
                     /locus_tag="VFQ10_11605"
     CDS             5177..5680
                     /locus_tag="VFQ10_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966032.1"
                     /translation="MLAAVSFMILSLAAVVFVWPGDFSPVEELISPGAKDIAVSQEDR
                     VYPTSDRLRFDGAPEVVYVYLRVEDFATYEDLEARVGRTSRVSALGRLLGGGGGELVV
                     SKEGEERLSVAYGGASGVVKFAVRPGSGGRLPSGNYTVAVYAASGGAGENSVVARKYF
                     VVGARHG"
     gene            5709..6446
                     /locus_tag="VFQ10_11610"
     CDS             5709..6446
                     /locus_tag="VFQ10_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HET9966033.1"
                     /translation="MRSRRRSGRPRREGTIARITDTVINVLSLLVVGVGVVVTASFFV
                     GSPQHDAEAEVAVDEKVSTATEQPRLPVAAEKIDRAIKRQRNDSEGAVARKRVASRGP
                     ESRMLRMTIPKMAQIRNDTVPYSVSDDNKAFRKHAAVHLRGTGNPWDRQANVYIAGHR
                     LGFPGTDSWLSFWDLNVLDKGDRVFITDSAGKRYVYKVFKTFVVDPEDVSVTRPLQGR
                     NIVSLQTCTLPDYSRRLIVQAEKVAER"
     gene            6542..>7801
                     /locus_tag="VFQ10_11615"
     CDS             6542..>7801
                     /locus_tag="VFQ10_11615"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9966034.1"
                     /translation="MTKGNMQVRVVSLAVLIAAVFLILGARLWYLQVLTGQDYTLAAK
                     ATQHTNIKIPAQRGVVYDRDGKILANNVPGLNVTVIPDEISRDKVRELATVVEADTEA
                     VLARYDAAKETGNQYSPFLVKENASRDAVTYVSERTGEFGGIAVNDDWVRSYPEGRLA
                     SHILGYTGAVTHDELKQEPFEGLPADSIVGKSGVELSYEEVLRGKAGQRTYNVDALGR
                     IVPEGSRVDSMGRFVDESGNPINVNPSQELPDHITEPEPGNNLTLTIDIDLQKVVEKE
                     LDAALERTWEAGYQGRGGAVVAMNPENGEILAMASRPDFSPKLFVGGISGGEELATYN
                     RLVSKRSNDPFLNRAIVGGQPGASTFKIFTGIAGLAAGVIDAYTIVNDTAIMNGTGCW
                     MPSSVSASPCYRSWRQNSPNYQFLGDHG"
BASE COUNT         1484 a         2378 c         2545 g         1394 t
ORIGIN      
        1 ccctcggggc gggtaaagac gccacgctcc gcgacgcgat gcgcttctcg gcggcgcgcc
       61 acgccctggc ccgagagtac gccagggact tcgaggtcac ccgcgagctc gccaggccgg
      121 cgctcctgtc ctctctttcc agggctgact ccgtccgcgg cgcccttgta caggcctacc
      181 tcgaggtgct ctcggaagtc cccgacctcg atgtggcagg ccgcgcgggc cgccaggagg
      241 ccgaggaagt ctcccgcatg gcgcacgggg tcttgaaggc cggcggcatc cactccaggc
      301 ggggcctcga agggatagcc aacctcggcg gcctgctccg cgcagactct cgcctctctc
      361 cgactacgac cgagtatcca gttatcgccg ccgcgttcct cgtggccatg gagtacggcc
      421 ctgacgccct gagccacagg ctaaggccgg ccacgggaag gtgaagccgt tcgcccggcc
      481 aattctctcg attggtagaa tctggctgga cgatcggcgg tagaggagga gcatgagggc
      541 tctggtcatc gtgggacacg gatcccacct caacggagac tcgagcctgc ccgtctacga
      601 gcatgccggc cgtatcaggg agacaggaga gttcgacgag gtcgtggaat gcttctggaa
      661 ggaagagccc tcaatgcgac atgtgctcga caccatagag tcggaagacg tctacatcgt
      721 gcccgcgttc atctcagaag gatatttcac ccagcaagtc atcccgaggg agctaggtct
      781 ggaggggccc gtctccacga agggcgagaa aaccgtccgc tacgccggac ctctcggcac
      841 cttcgagggg atgcctgatg tcatactgga gcgtactcaa gacctcatgc gcggaaagga
      901 ggtctcggga cgcacggccc tcatcttgct cggccatgga accgagctga acaagaactc
      961 aggcggcgtc atctacctca acgccggacg catcagggag cgtgggatct acgacctcgt
     1021 cgaggtcggg ttcctggatc aagaccccga gataggcggc gtcgtcgatg gagtcgaggc
     1081 gcagaacgtg atcctgatcc ccttgtttat cgcggaggga tggcacacac gcgagacgat
     1141 ccccgaggac cttggccttg tgggcgaggt tacggtacgc gacgataaga cgatcttcta
     1201 cggcgccccc gtcggaacgc acccttccat ggcgaacctg atagccgccc gcgcccgtgg
     1261 gatgatgaca gagcagcctg ttgtctgacg ctcgctccgg gtgccctgag aacgtcgctg
     1321 cgagttcgcg ccaggccttc gcggcatggg tggatgatgc gcccgaaggc cgcgtcttcg
     1381 ggcaggtagt cgtacgcccc gttccgcccc ttggatactc actgcgtcac gtggacgagg
     1441 cgcgggagga gggtctcagt gtcagcgatg acccacacga tgcgcgagag atcgccaggc
     1501 tcaccgaagg cggggagtac aggccgctga agagctcccc taacctgcgg cgcggctggg
     1561 agctcagggt cgaggacgcg ctctcactgg tgaccgccat gaactacctc tatcccgctg
     1621 gcgtcgtcca ctggtacctc cacagggagg ggaggctcca cctcacgagc ttcagggagg
     1681 cggcggcgag gcagagtggg atatacaaac gggttcagcg gctcacggac cagggggtgc
     1741 tcgatgcggc cctcgcctgc tgcaaggact cagtgtgtct caagaagacc ctctgggatg
     1801 tggacgagga cacgccgctc gacgtagacc ggggcgaagg tgagatcccc tgccccgagc
     1861 cttgcagtat cttcatctcc tttgcgcggc gggtgaggct cttcgagcgg gagaaagacc
     1921 tcgatcgcac gggcctctcc ccctcggaga cggaagacct tgccgccctc gtcgaggccg
     1981 cggccacagg agaggtcaag ttcgcccgcg aggccgagtt cgagaagccg ctcaacgaga
     2041 gaaggatgcg ctacaggagg ctcaccctcg ttcccaaact gcgccggaac ggacagagtg
     2101 cctcctgaag cctcgaacga cgcgacgacg cttatcccag gtctgttttc gaacgggcct
     2161 ttcggggtat ctcgtagttt cgagaaacca actccgcgaa gatggaggta gtgaatggca
     2221 cgcaagaagc gaagagccgc cagagccggt gcgaaggcag gagcaagggt ggcccccgtg
     2281 gccggcagga ccgtagggaa ggcagcctgg aagctcggca aggccgaagc caagctgata
     2341 cgccgggcga tgagctcgca ggaaccgaag aagacccgct atctcaagta cgggttcttc
     2401 atcctgatcg ggctcgctat cggggccgcc atcgcacgtt cgaagaaagg tgaatcatcg
     2461 gacgagtcct cgtcttacac gggtactaca ggcccccatg ccccagaccc tggaagccct
     2521 gcaggagagc ggggccagac ctggggtagt ggcaccccga cagggacggc tggcggcggt
     2581 ggccaacaga gtccgggggg tgcgaaccgc accggcgccg atcgggagta ctcggacccc
     2641 tcgtccgggc ctctgatcgg cgagcagcat ggcaacgtcg agggcgtagg cgagcagcag
     2701 gaggagatag aacagcgcat ccgcacccac ctcggtgagg acccacgcac cgcggagatg
     2761 ccgcgggtga acgtcgaagt aaacgacgga atcgccgagc tccgcggggc tgcgccatcc
     2821 gaggatgcca agctggctgc cggtgagata gcccaacagg tcgaaggcgt ccgcgaggta
     2881 cgaaacctga tacaagtaag ctagtcagca cgcttcgggc ctgcggccct cgcttgtcag
     2941 gcagttggat cgcgtcgcgg gctgctcctg ccagtacaca cgccccgtca tctcgatggg
     3001 gcactttctt cccccgattc tcctcaagag gccgatgctg acaagcacaa atgttgacga
     3061 gcgcccgaca gggcgtgtgc tgaatgctgc tcaatcgcga acgaggttga gcagctcgtc
     3121 gcgtttgcgc accatcgtct cgttgtcttc ggcctccacg ttgaggcgga ggagaggttc
     3181 ggtgttcgag gggcggaggt tgaaccacca gtcgccaagg tcgactgtga ggccgtcgag
     3241 gtggtctacc tttgcgccgt cgttattgga gaagtggtct tcgatcttct ccagggtcgc
     3301 tccctggtcc tctacttcgg agttgatctc acccgagcgg acgtaaggat cgatggggga
     3361 gaggagcctg gagataggct cttcctggcg gccgacgagt tcggctatgg ttagcatggc
     3421 gatgatacct gagtcggcga agtagttgtc cctgaagtag aagtgggctg aatgctcccc
     3481 gccgaaggcc gcgtcgtgct tccgcatctg cggcttgatg atggagtggc ctgctctggt
     3541 ccttatggcc ttcccgccct cgcgctcgac gagctcgggg aaggccttcg agcagaccgc
     3601 gctgtacagt atggtcgcgc ccggcttctt ctcgagcacg ttcttcgcta tgagggcggc
     3661 cgccaggtcg cccgagatgg tcgtgccgtt ctcgtcgata atgaagcacc gatcggcgtc
     3721 accgtcgaag gctaccccga aatcagcacc ctctgcgacc acgcgctcct ggagctctct
     3781 gacgttctcg ggttcgatcg ggttcggtgg atggttgggg aaggagccgt cgagctcgaa
     3841 gtacatcggt acgtactcga aagggaggtg ctcgaagatg ggtgggacca tcttgccagc
     3901 catgccgttg ccggcgtcga tcacgatctt cagtggcctc aagccctccg tctgtataaa
     3961 gctcaggcaa tgtttcgcat agtctacggt gatgtccgtc tcctcaacgg aacccgggta
     4021 ttccgcgggg ccggggagat cccctgagac gatgaggtca cggatctggg cgagccccgc
     4081 gtcccccgag agggcgatgg cttcctccct gcagagtttg aagccgttgt agtctttggg
     4141 gttgtgcgag gccgttacca tggccccgcc tggctcctcg aggtgtccta cggcgaagta
     4201 gagcgcatcg gtcgagacca gcccaaggtc taggacgtct gcgcccgctt gcgtaacccc
     4261 ctgtataaag gccttctcca acgcctcgcc cgaaatgcgc atatcgcggg ccacgacgac
     4321 cctggagccc gagaggtcca ggtgctttac gaaagcgcgg ccgatgtcgg tggccacgcc
     4381 ctcatccagg ctctcgggat agacgccacg gatgtcgtag gccttgaata tcgactcgtc
     4441 gagcatttcg aacctccgga ctcctcccgg tctgcgtgcg acctgtggtt ttctccgtcc
     4501 ggtacaataa ccggttatgg tccatcctgc agaccggttc acagcgtacc caggttaggc
     4561 gcgccttgag cccccgcttc gtcaggttcg cagccctgtt ctacggttct ctcgctgtcg
     4621 tcgccgccct gtggtgcggc ctcaggggct tcaacgtgcg gcttctcggc gagagtccag
     4681 gtgcaagcgt gctactgggt gtcattacgg ccgcctgcac agtctccttg ggtcttcttg
     4741 cctaccggct tctacccacc ttgcgtgaga tctccgacga actcgccccg cgcctggtcg
     4801 acggcgccgc ttcgtccagc ctggtgctcg tcgcgatctt ctccggggtg ggggaggaag
     4861 cgtttttccg gggggcgctt cagcaggagt tcggcctggt ggtcgcctct ctgttgttcg
     4921 gcctggccca tattggcccc gaccggcgtt atctcgtatg gacggcgtgg gccgtactgg
     4981 tcggcttcgt tttcggtttt ctctacgagg tcagcggcgg tctgctcgct cccatgctcg
     5041 cccacaccgc acacaacgcc gcgacgctcc tgctctggaa acgctcccgc aaggacccga
     5101 agaaggccgg tggcctctga acaagctccc caacactacg acaggcaaga ccggagaccg
     5161 ttcccgtgtt ggtcctgtgc tggccgcagt gagtttcatg atcctgtcgc tcgctgcagt
     5221 cgtattcgtc tggccagggg acttctcgcc ggtggaggag ttgatctcac ccggggccaa
     5281 ggacatcgcg gttagccagg aggacagggt gtacccgacc tcggacaggc tgcgtttcga
     5341 cggggcgcca gaggtggttt acgtctatct gagggtcgaa gacttcgcta cctacgagga
     5401 cctcgaggcg agagtagggc ggacgtcgag ggtctccgcc cttggccgtc tcctcggtgg
     5461 cggcggaggc gagcttgttg tctcgaaaga aggtgaggag cgactcagcg tggcatatgg
     5521 cggggcctcc ggcgtggtga agttcgccgt ccgccctgga tcgggagggc ggctgccttc
     5581 aggcaattac acggtggcgg tctacgccgc atcgggaggg gccggcgaaa acagcgtggt
     5641 tgccagaaaa tacttcgtgg tcggcgccag gcacggctag tagtcgtccg cttttgggat
     5701 acaatggatt gcgatctagg cggcggtccg ggcgaccgcg tagggaggga acgatagcgc
     5761 gcatcactga cacggtcatc aacgtgttga gcctcctggt cgttggcgtg ggagtcgtgg
     5821 tgacagcgag cttcttcgtc ggctccccgc agcacgacgc cgaggccgaa gttgcggttg
     5881 acgagaaggt tagcacggct accgaacagc ccagactccc tgtggccgcg gagaagatcg
     5941 acagagccat caagaggcag agaaatgact cagaaggggc ggtggccagg aagagggtcg
     6001 ccagccgggg gccggagagc aggatgctaa ggatgaccat acctaagatg gcccagatac
     6061 gcaacgacac cgtaccctac tccgtaagcg atgacaataa agcgttccgc aaacacgccg
     6121 ctgtgcacct ccgcgggacg ggcaacccct gggacaggca ggcgaacgtc tacatcgccg
     6181 gccaccggct cggtttccca ggaaccgaca gctggctgtc gttctgggac ttgaacgtgc
     6241 tcgacaaggg ggatagggtc tttatcaccg actcggcggg caagcgttac gtctacaagg
     6301 tgttcaagac ctttgtcgtg gacccggagg acgtgtcggt gacgcggccc ctacaaggca
     6361 ggaacatagt gtcgctacag acgtgtacgc tgccggatta ctccaggcgt ttgatcgtcc
     6421 aggccgaaaa agtggccgag cggtagtgct cgctacagaa gaggggtaga ggttgctccg
     6481 gctgccggtg agagagaata agcaggcgaa ccccgcgaaa aaacgtgtgg agacgccgcc
     6541 catgaccaag ggcaacatgc aggtgcgggt agtgtctctg gcggttctga tcgctgctgt
     6601 cttcctcatc cttggcgcca ggctgtggta cctgcaggtg ctaacaggcc aggactatac
     6661 cctcgccgcg aaggcaacgc aacacaccaa catcaagatc cccgcccagc ggggcgtagt
     6721 ctacgaccgc gacggcaaga tcctcgctaa caacgtccct gggctgaacg taaccgtcat
     6781 accggatgag atctccaggg acaaagttag agagctggcg acggtagtcg aggccgacac
     6841 cgaggctgtc cttgcccgct acgacgcggc gaaggagacc ggtaaccagt acagcccctt
     6901 cctggtaaag gagaacgcga gccgcgacgc ggtgacctac gtgagcgagc gcaccgggga
     6961 gttcgggggt atcgccgtga acgacgactg ggtccgaagc taccccgaag gcaggctggc
     7021 ctcccacatc ctcgggtaca ccggtgctgt aacccacgat gagctgaagc aggaaccttt
     7081 cgaaggcctg cccgcagact ctatcgtcgg taagagtggg gtcgagctct cctatgagga
     7141 ggtgctgcgc ggtaaagcgg gccagagaac ctacaacgtg gacgccctcg ggcgcatagt
     7201 ccccgaaggt agcagggtcg attccatggg caggttcgtg gacgaaagcg gtaaccccat
     7261 aaacgtgaac ccaagccagg agctacccga ccatatcacc gagcccgaac ctggcaacaa
     7321 cctcaccctc actatcgaca tcgatttgca gaaggtcgtc gagaaagagc tcgacgcagc
     7381 cctggagcgc acctgggagg ctggctacca gggcaggggc ggggctgtcg tagccatgaa
     7441 ccccgagaac ggggagatcc tggcgatggc cagccgtccc gacttcagcc cgaaactgtt
     7501 cgtcggcggc atctccggcg gagaggaact cgccacctac aaccgcctcg tatccaagag
     7561 gtcgaacgat ccgttcctca accgcgcgat cgtcggtggg cagcctggtg cctcgacgtt
     7621 caagatcttc acggggatag ccggcttggc cgccggcgta atagacgcct acacgatcgt
     7681 caacgatacg gctatcatga acgggacggg ctgctggatg ccgtcgagcg tctcggcgag
     7741 cccgtgctac aggagctggc ggcagaacag ccccaactac cagttcctcg gcgaccatgg
     7801 c
//
LOCUS       DASTKM010000289         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1346409, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000289 DASTKM010000000
VERSION     DASTKM010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346409"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFQ10_11620"
     CDS             complement(<1..373)
                     /locus_tag="VFQ10_11620"
                     /inference="COORDINATES: protein motif:HMM:NF027404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966035.1"
                     /translation="MSQPNGPAGEGFHGPNLAYVLEMYEDFRADPVSVDESTREFFER
                     WSPPADVAAGEGSPAAALEKALKAARLVGGIRSFGYAAARLDPLGSTTPSDPVLEAEF
                     DGLSEEDLEYVPAGPVVGRPVG"
     gene            862..2952
                     /locus_tag="VFQ10_11625"
     CDS             862..2952
                     /locus_tag="VFQ10_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET9966036.1"
                     /translation="MHSHERYRVGIDIGGTFTDLVLIDDETGEQAVGKILTTPDDPSQ
                     AVEVGLCEVLEREDVEAGQLGMVVHGTTLVTNALIERRGARTALLTTEGFRDAIAIGT
                     EHRYDMYDIFIEKPEPLVPRSLRYGVRERSLDDGSVLHQLDEEQVRSIAKELKEREVG
                     AVAVSFLHGFRNPAHEQRVAEILAEAAPEITISLSSEVSPEIREYERTSTTVCNVYVR
                     PLVERYLQRLEERLRKLGFEGSLYIMLSNGGTASVETASRFPIRLLESGPAAGALAAA
                     FYGRGSGFPDVLSFDMGGTTAKSCVVEDGEPLTSSEFEVARVYRFKKGSGLPVKTSVI
                     EMIEVGAGGGSIAHIDALGLLKVGPDSAGAEPGPVCYGLGGSEPTVTDADLILGYLDP
                     DFFLGGRMELDREGASRAIEQKIARPLGMDGVEAARGIHQVVNENMANAARIHAVERG
                     KDPRAYPLFAFGGAGPVHGYRVARALGVSGFIAPLGAGATSAFGFLCAPLAFDFKRSL
                     YGRLDELDWSEVNRALEEMEGEGRDLLRASGVLDAEIQVRRLGEMRYAGQGHEVTVEL
                     PDGELGPDDADRLATLYHEEYRRLYGREGPDVPLEALTWRLEVVSPRPQIQLDARKDN
                     PRALSEAHKGKREIYLPENEGFRNVPVYDRYRLDPGAAFEGPAVVEERESTVVLGPDT
                     EVEIDPARNLIVRW"
BASE COUNT          540 a          888 c         1001 g          532 t
ORIGIN      
        1 cacctaccgg tctaccgact acggggccgg ccggcacgta ctccaagtcc tcctcgctca
       61 ggccgtcgaa ctctgcttcg agcaccgggt ccgagggtgt cgtgcttccg aggggatcga
      121 gccgcgccgc cgcgtatccg aagctcctta taccccctac tagcctcgcc gctttgagtg
      181 ccttttcgag ggcagccgca ggcgacccct cgcccgccgc tacgtccgcc ggcgggctcc
      241 agcgctcaaa gaactcgcgt gtgctctcgt ccacggagac tggatcggcc cgaaagtcct
      301 cgtacatctc aaggacgtaa gcgaggttgg gaccgtgaaa gccttccccc gccggaccgt
      361 tcggctggct caagctcgat cacccctgtt cggcgtctct ttacggctat tttggatact
      421 gtataataaa ttccgcggtt gacgatggca aagtaaaggg gcgtctgggg caatcagagt
      481 actaggcggg gccatagcca actgcgcgcc tttgtgccga aaccgttcgg agggtttcgc
      541 gtcgctgtct ggtggcagag gaggtcgggt gcgccgcagg ggcgagccga gaccccaaag
      601 gtgggggtga gggttatttg gcgacggcga gcgtgttggg gggagtttgc ccccaccgag
      661 cggcacaggc acgatcggtc ggacgagtac aggcgggttg tggacgcctt ggaggggcga
      721 ggcatggatt accgggtggc ggtggagctc gccgacccgg cctcacgctg tacggaagga
      781 ccggagacgc cgtcatacgg acgtaactcc gtctgcttgc tactctgatg aggtcttgca
      841 gaaagaatcg aggtgtgaga cttgcactcg catgaacgct atcgcgtagg catcgacatc
      901 ggcgggacgt tcacggatct cgtcctcatc gacgacgaga cgggcgaaca ggcggtcggc
      961 aagatactga ctaccccgga tgatccgtcg caagccgtcg aggtagggct atgcgaggtg
     1021 ctcgagcggg aagatgtcga ggctggtcaa ctgggtatgg tcgtccacgg aacgacgctt
     1081 gtcactaacg ctctgatcga acgccggggc gctcgaacag cgctcttgac tacggagggc
     1141 ttccgggatg cgatcgccat cggcaccgaa caccgctacg acatgtacga catctttatc
     1201 gagaaaccgg agccgctggt gccgcgcagc ctacgttacg gtgtgcgcga gcgctcctta
     1261 gacgacggct cggtactaca ccaactggac gaggaacagg tacgatcgat agcgaaagag
     1321 cttaaggagc gcgaggttgg ggccgtggcc gtgagcttct tgcacggctt ccgcaacccc
     1381 gcccacgaac aaagggtcgc cgagatcctg gccgaagcgg cgccggagat cacgatttcg
     1441 ctctcttcgg aggtgtcgcc cgagatcagg gagtacgagc gcacctcgac gacggtctgc
     1501 aacgtctacg tccgtccgct ggtcgaacgc tatttgcaga ggctcgaaga acggctgcga
     1561 aagctcgggt tcgagggttc gctgtacatc atgctctcca acggcgggac ggccagtgtg
     1621 gagacggcca gtcggtttcc gattcgccta ctggagtccg gccccgcggc gggcgcctta
     1681 gccgcggcct tttacggtcg ggggtccggt tttcccgacg tgctctcctt tgacatggga
     1741 gggacgacag ccaaatcctg tgtggtggag gatggagagc cgctgaccag cagcgagttc
     1801 gaggtcgcca gggtctaccg gttcaagaaa ggctctggat tgccggtaaa gacctccgtg
     1861 atagagatga tcgaggtcgg cgctggcggt ggcagcatcg cccacatcga cgctctgggc
     1921 ttgctcaaag tcgggcccga tagtgccggc gccgagccag ggccggtttg ttacggcctt
     1981 ggaggcagcg agccgaccgt gaccgacgcc gacttgatcc tggggtattt ggatcccgac
     2041 tttttcctcg gcggacggat ggagctagac cgcgagggag cttcgagggc gatcgagcag
     2101 aagatcgccc gcccgctcgg catggacggc gtcgaggccg cgcggggtat ccaccaggtc
     2161 gtcaacgaga acatggccaa tgccgcgagg atccacgccg tcgagcgcgg caaggacccg
     2221 cgggcgtatc ccctgttcgc cttcggcggc gccggccccg tacacggcta ccgcgtcgcc
     2281 cgcgccctcg gcgtctccgg cttcatcgcg ccgcttggcg cgggcgctac ctcggccttc
     2341 ggtttcctat gcgccccgtt ggcgttcgat ttcaaacgca gcctctacgg tcgtctggac
     2401 gagctcgact ggtccgaggt gaaccgggca ctcgaggaga tggaagggga agggcgggat
     2461 ctgctgcgcg cttcgggcgt cctcgacgcc gagatccagg tacgacggct cggcgagatg
     2521 cgatacgcgg gccagggtca cgaggtaacg gtggagctgc ctgacggcga gctcggtcca
     2581 gacgatgccg accggctggc cacgctctat cacgaggagt accggcggct ctatggacgt
     2641 gaagggccgg acgtgccact ggaggcgctg acctggcgtc ttgaggtcgt ctcgccgcga
     2701 cctcagattc aactcgacgc acgcaaagac aacccgcgtg cgctctcaga ggcgcacaaa
     2761 ggcaagaggg aaatatacct gcctgagaat gagggcttcc ggaacgtacc agtctacgat
     2821 cgctaccgcc tcgatccagg tgccgctttc gagggaccgg cggtggttga ggagagggaa
     2881 tccaccgttg ttcttggccc agataccgaa gtcgagatcg acccggcccg aaacctgatc
     2941 gtgaggtggt aggcatggca c
//
LOCUS       DASTKM010000290         9104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_996701, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000290 DASTKM010000000
VERSION     DASTKM010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996701"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..392
                     /locus_tag="VFQ10_11630"
     CDS             114..392
                     /locus_tag="VFQ10_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966037.1"
                     /translation="MRLVAAEEGVPGVLWAVLIFGGIAAVSFTYLFGLENTWAHRLMV
                     FTLAAVIGLVLFTIGALEHPFSGGARIGTEAFDLVLERFETSKLSDLR"
     gene            717..3431
                     /locus_tag="VFQ10_11635"
     CDS             717..3431
                     /locus_tag="VFQ10_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9966038.1"
                     /translation="MADRCEPVGRDEGELGTLRSVIDGLWAHGESPALVAVGEEGVEI
                     WTYGELAGRVLGLGRGLRVAGVERGDHVALLAANRREWIAACLAVISAGAVVVPLDAQ
                     LGDEALGHVLEDSGARHVFTTAEDAGRLENVDTGAALRTILLDVGEEDRRSWQHLLTD
                     EDVDLPPGGPGDVAALFYTSGTTGAAKGVPLSHANLVFQIDTLLGADLVSEEDSVLLP
                     LPLHHVYPFVMGMLTPLAAGLPIVLPQSLTGPQLVRALSEGEATLIVGVPRLYGALYS
                     GIEARVGSGGRFAAIHFKCGIELCVKLRRMTGLDVGRVALRPLRKRFGPRLRVMAFGG
                     AALDPDLAEKLEGLGWRVAVGYGLTETAPLLTLNPPDGRKLGSVGRPVPGVEISIDHS
                     AMPEGAVRAGTAHREGEILARGPNVFSGYRNRPEETSKVFDGGWFRTGDLGYFDDDGY
                     LYVTGRASTLIVTSGGKNIQPEAVEAAYLENPLIREIGVLQKDGRLVAVIVPETGEIS
                     RGTDTHRVIREAVEEASKHLPSYQRISDYAISREPLEFTRLGKLRRHVLEERYERARR
                     GVESQDRTAVGPVAPQEMSGEDRALLEDPTALGVWKLLAERYPDRRVAPETSTQLDLN
                     IDSLGWVNLTLEIGEKTGVELDEEAIERIDTVRDLLREVASGASGTLHPVSPFERPEE
                     VLDDRQKRWLEPLGPARSAMARGMFALNRMMMRGPFSLQVEGLENLPDRGPFIIAPNH
                     VSYLDAFAVTAALDYSVLRRTYWAGWTGAAFGNPLTRLVSRLAQVVPIDPGRAGLSNL
                     AFGAAVLRRRQNLVWFAEGERSPTGSLQPFKPGMGILLSYYPVPVVPVFIRGTYEAMP
                     RGRFLWRLEQVTVSFGEPVDPDGLDEASSSQEQMVQAVHKRVAALGER"
     gene            complement(3495..3821)
                     /locus_tag="VFQ10_11640"
     CDS             complement(3495..3821)
                     /locus_tag="VFQ10_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966039.1"
                     /translation="MCLDMLRSRNSQRKEPDLATQRKVVDAFLAASRDGDFDALVALL
                     DPDVLLRADRGAMPAGAWREVRGAEAVAGQALNYSQLALLVRPALVNGAAGLVGRDDR
                     SRIRRE"
     gene            complement(4028..4351)
                     /locus_tag="VFQ10_11645"
     CDS             complement(4028..4351)
                     /locus_tag="VFQ10_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET9966040.1"
                     /translation="MPTRGERVITEERVQDQLRNVIDPEIGMDLVELGLIYDVGVHDE
                     GRHVDVTFSLTSPMCPVGDLIQEQVETETLSIEGVETVNAQLTFEPMWSPEMMSPAAK
                     LFFGR"
     gene            complement(4468..6081)
                     /locus_tag="VFQ10_11650"
     CDS             complement(4468..6081)
                     /locus_tag="VFQ10_11650"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HET9966041.1"
                     /translation="MGERTRLGLGILGVALALGGLGDVLLRATPWGINFLIWVMAIVG
                     LTVLLAKWGRVGTGGEGKWLVFVSLVFAAGVVLRDSPVVVFLDLLGVLISLSLAVWLG
                     RSGSLRRAGILDYVLGGVYTGVLTSAGPFPVSVADIEWRETFRGRWKGQALAVSRGVL
                     LAAPLLLLFGALLVAADSIFERLIIEVFGFDLAEVFSHLFLITFFAWITAGLLWAGLM
                     ARVPENFAIPRPRVLSLGIVEVGIVLGLLDLLFLTFVVIQVRYLFGGAGRVAATAGLT
                     YAEYARRGFFELVTVTALALPLLLIAHWLLRAETRAHERLFKAFAGIMVVLLFVVVAS
                     ALQRMYLYQQEFGLTELRLYTTVFMVWISVVLLWFLLTVMRARRDHFAFGALLAGFAA
                     IFAINVMNPDALIASTNIDRMEDGKRFDAYYLTTLSADAVPVLVESLPEIGDTHMWTG
                     YTVEQAVVDRWDAKRADWRTWNLGRSRARQVVSSYVEDKDVRASRMISFDPRHQDRAF
                     MHRPDRVIHNMLWIRSPQRGISGSSVTSA"
     gene            complement(6278..7534)
                     /locus_tag="VFQ10_11655"
     CDS             complement(6278..7534)
                     /locus_tag="VFQ10_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966042.1"
                     /translation="MSPRAPRPPALRRLVLARAAWVAVAFVSLGTFVISVPARYAQLA
                     NPTAGVRAALAEMGLSAGGYALYNVTLDTVFVSVFALVAIVIFWRRSDDPMALLVATM
                     LVVWGSLNGLFVLTPSATEGMYPVLQATLGSLVTFVGYIAWMLFFYLFPSGRFVPRWT
                     LWMALLYGVAFFGLWNFTPFGPPSWPPLLFNAAVVVVWGSFLVAQLYRYARVSDATQR
                     QQTKWVVFGVAVAIVGTLTTIFTVGAAVDLPPEEVGSKMLSMLLMDAFGLFIPLSIGI
                     AVLRARLFDIDVVINRTLVYGSLTVILALVYFGGVAMTEVIFRGLTGQEQQPQLAIVI
                     STLVIAALFNPLRRRNQSFIDRRFYRRKYDARKTLEEFSARLRDETDLEALNADLVGV
                     VRETMQPVHVSLWLRPGTAPQREVVD"
     gene            complement(7722..>9104)
                     /locus_tag="VFQ10_11660"
     CDS             complement(7722..>9104)
                     /locus_tag="VFQ10_11660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET9966043.1"
                     /translation="TSGASARLVHGGLRYLGTGDFRLVRTALRERDVFASRVAPHLVR
                     PLPFVLSAAGGRRQRSRCAAGLLVYTALDGFRSPLPRFVTPEEATLLVPPLSVGVTAS
                     HAVYFEAGTNDSRLTLATVTAAARSGAVVANYLRAVNLDLAPGRISRVSLQGRGVEIT
                     VRCRAVVNATGPWLDLLRNMEDSACEPVTRLSKGIHVVLRPDEQWRAAVAVSLEGGQH
                     LYAVPCEDMILLGTTEEEYHGDPAAVAAEPEDVSYLLQVARRFLRPQMLRRERVISAF
                     AGLRVLPMGEETTLHASRNHLLSVGPGGMVSVAGGKLTTHRRIALDALRHLPARVRPR
                     RLSLSDAPLPGASPVRDLYTRLDGSKVDHLLHLYGSETGKLLGYSKVKDNALERIMPG
                     APDLWAQIYHAVHEEWALTAEDVIYRRTTLGLRGFDTPEIRQRISTVLEAGAQIPSPG
                     LFEPPLKRVV"
BASE COUNT         1789 a         2780 c         2900 g         1635 t
ORIGIN      
        1 ttatcgacga gatccgggcg aaccttcaag agttccagcc acgcacacca gccgaccagc
       61 aactctacgc agaaggactc gaccaggtcc agtgactggc cgacgcaagg agaatgcgcc
      121 tggtcgcagc cgaagagggc gtccccggag tgctctgggc cgttctgatc ttcgggggga
      181 tagcagccgt aagcttcacc tacctcttcg gcctggagaa cacgtgggcg cacaggttga
      241 tggtgtttac cttggcggcg gtgatcgggc ttgtgctgtt caccataggc gccctggagc
      301 accccttctc cggaggtgcc cgcataggca cagaagcctt cgatctcgta ctggagcggt
      361 tcgagaccag caagctcagc gatctccgat aagctctgtt tcagcctgtc aacactctct
      421 ggctgacgcc tccacttgtc agcatttcac cttcttgttt ccgcttgtag ctgaatgggc
      481 gcttcgcagt aggtcaatgt ctctgcggat cgctctgagc ctacgcaaac atgctgttgc
      541 cgcccaagcc tcgaggccat atttccgcaa acgcccgcaa gtgatcctta cacgtcgccc
      601 ctgacatcag ccgaggcctc gaacgctacc cataaatctc ccaaaaaccc ttgacaaccg
      661 acgatgtgcc actctatact tacggtgtaa atttacaacg taaggagaga aggatgatgg
      721 ctgatcggtg tgagccggtg gggcgggacg agggcgagtt gggcacgcta aggtcggtga
      781 tcgacgggct ctgggcgcat ggtgagagcc cggcgctggt ggcggttggc gaggagggcg
      841 tggagatctg gacatatggt gagcttgccg ggcgggtgct tggtcttggt cgtggcttgc
      901 gtgtggccgg ggtggagcgg ggcgaccacg tggcgcttct tgccgctaat cgtagggagt
      961 ggatcgcggc ttgcctggcg gtgatctcgg ctggggccgt ggtcgtgccg ctggacgccc
     1021 aactcgggga tgaggccctc ggtcacgttc tcgaggacag cggcgccagg cacgtcttca
     1081 ccacggctga ggatgccgga cgtctggaga acgtagatac gggtgctgcg ttgaggacca
     1141 tcctgctcga cgttggagaa gaggaccggc ggagctggca acacctgcta acggatgagg
     1201 atgtggactt gccgccgggt ggacctggcg atgtggccgc tctgttctac acttcaggca
     1261 caactggcgc ggcgaaagga gtgcccttga gccacgccaa tctcgtgttc cagatcgaca
     1321 ctctgctggg cgcggatctg gtatccgagg aagatagtgt gctcttgccg ctgccgttac
     1381 accacgtcta ccccttcgtc atggggatgc tcacgccgct ggcggcaggt ttgcccatcg
     1441 tcttgcccca atcgcttaca ggtccgcaac tggtgcgcgc cctgagcgaa ggagaggcta
     1501 ccctgatcgt cggtgtcccg cgactctacg gtgcgctgta ttcaggtata gaagcgaggg
     1561 tgggctccgg cggacgtttc gcggctatcc acttcaagtg tggcatcgaa ctttgcgtca
     1621 agctgcgtcg aatgaccggg ttggatgtcg gtagggttgc cctgcgcccc ctgcgcaaac
     1681 ggttcggtcc tagactacgg gtaatggctt ttggcggggc ggccctggac cccgacctcg
     1741 cggagaaact ggaggggctg ggatggcggg tcgccgtcgg ttacggcctc accgaaaccg
     1801 ccccactcct gacgctcaac cctcccgatg ggagaaagct cggcagtgtt ggacggccgg
     1861 ttcccggcgt cgagatcagc atcgaccact ctgcaatgcc cgagggggcg gtcagggcgg
     1921 gcacggctca cagagaaggt gagatcctgg cccgcgggcc gaacgtcttc tccggatacc
     1981 gcaaccggcc cgaagagacg agcaaggtct tcgatggtgg atggtttcgc acgggggacc
     2041 tcggctactt cgacgatgac ggttacctgt acgtcacggg ccgggcgtca accctgatcg
     2101 tgacctccgg tggcaagaac atccagcccg aagccgtcga agcagcctac ctggagaacc
     2161 cgttgatccg tgagatcggc gtgttgcaga aagacggccg gctcgtcgca gtgatcgtgc
     2221 ctgagacggg tgagataagt cgaggtaccg acacccaccg cgtgatacgt gaggcggtag
     2281 aggaggcgtc gaaacacctg ccttcctacc agcgcatctc cgactacgcc ataagccgcg
     2341 aaccacttga gttcacgagg ctcggtaagc tcaggcgcca cgtcctggag gagcgctacg
     2401 aacgagccag gagaggcgtg gaaagccagg ataggacggc cgtagggccc gtcgcgcccc
     2461 aggagatgtc aggggaagac cgagcgttgc tcgaagatcc tacagcgttg ggcgtctgga
     2521 agctcctggc cgaacgctat cctgacagga gggttgcgcc tgagaccagt acgcagctcg
     2581 acctgaacat cgattcgctg ggatgggtga acctgacgct ggagatcggc gagaagaccg
     2641 gcgtggagct ggacgaagag gcgatagaac gcatcgacac cgttcgcgac ctgctgcggg
     2701 aggtcgcctc gggagccagc ggaacccttc atcccgtttc accattcgag cggcccgaag
     2761 aggtactcga cgaccggcag aagcggtggc tggagccgct ggggccggca cgctcggcga
     2821 tggccagagg catgtttgcc ctcaaccgga tgatgatgcg cgggccgttc agcctgcagg
     2881 tggaaggcct ggagaacctg cctgatagag gtcctttcat catcgcgccg aaccatgtca
     2941 gctacctgga tgcgttcgcg gtgaccgctg cgctcgacta cagtgtgctg cggcgcacgt
     3001 actgggcggg ctggacgggc gcggccttcg gcaatccgct tacccgtctc gtcagccgtc
     3061 tcgcgcaggt ggtcccgatc gaccccggcc gcgcgggcct ctcgaacctg gccttcgggg
     3121 cagcggtact caggcgcaga cagaacctgg tctggttcgc cgaaggtgaa cgttcaccga
     3181 caggaagctt gcagccgttc aagcctggca tgggaatact gttgagttac tatccggtgc
     3241 ctgtcgtgcc cgtgttcatc cgtggcacat acgaggccat gccgagggga aggttcttgt
     3301 ggaggctgga gcaggtcacg gtgagcttcg gcgaaccagt ggaccccgac ggcctcgacg
     3361 aggcctccag ctcgcaagag cagatggtcc aggccgtgca caagcgtgtt gcggcgcttg
     3421 gcgaacgttg acggcccgcg gtgggctacg ttcattagag aactctagtc tctcgatcac
     3481 cgctgtagta tgactcactc tctcctgata cgagatcggt catcgcggcc cacgaggccg
     3541 gcggcgccgt tgacgagagc cgggcgcacc aatagggcga gctgcgagta gttgagcgcc
     3601 tggccggcta ccgcctccgc accccggacc tccctccatg cgcccgcagg catggcaccg
     3661 cgatcggcgc ggagcaatac gtcagggtcg agcagcgcga cgagcgcgtc gaagtcaccg
     3721 tcgcgcgatg cagcgaggaa ggcgtcgacg acctttcgct gggtggcgag gtcgggctcc
     3781 ttgcgctgcg agttgcgcga gcgcagcatg tccaggcaca cccgccggcg cgttccgggc
     3841 cggcgctaac ctgcctctcg aacaggccgt cggccaccgg acctgggagg agttcctcgc
     3901 ggaccggctg aattagcagg aacgttgcag ccgactcact agggcgtgca cgacataaag
     3961 atgtagcgtc ccggcttcct ccctctcggg tctcaccggg acgggattgt cggccgggat
     4021 atcgtgctta gcggccgaag aacagtttgg cggcagggct catcatctcg gggctccaca
     4081 tcggctcgaa cgtgagctgg gcgttcacgg tctcgacccc ctctatggag agcgtctccg
     4141 tctcgacctg ctcctggatc aaatccccaa cggggcacat tgggctggtg aggctgaagg
     4201 tgacgtcaac gtggcggccc tcgtcatgca cgcccacgtc gtagatcagg ccgagctcga
     4261 cgagatccat cccgatctct gggtctataa cgttcctcaa ctggtcctgt acgcgctcct
     4321 cggttatcac tctttcacct cttgtcggca tcagttcttc acgctgattg taaagcctcg
     4381 gagaggcttg tccagatgcg tcgccgatgg agcagttcag cgagacatag cttgccagtc
     4441 tgaacgctgt aacccgatgc gcttttgtta agcgcttgtt acacttgatc cacttatccc
     4501 ccgttgtggg cttcttatcc acaacatgtt gtggataact cggtcgggac ggtgcataaa
     4561 cgccctatct tggtgcctgg ggtcaaagct gatcatcctg gatgctcgta cgtccttatc
     4621 ttcgacgtaa ctactgacca cttggcgggc tctcgaacga cccagattcc acgtacgcca
     4681 gtcggccctt ttcgcgtccc agcgatccac gacagcttgc tccacggtgt agcctgtcca
     4741 catgtgcgtg tcacctattt cggggagaga ctcgactagc acgggcacgg cgtcggcgct
     4801 cagggtggtc aggtagtagg cgtcgaagcg ctttccatct tccatgcggt ctatgttggt
     4861 gctcgctatc aaggcatcgg ggttcataac gtttatggcg aagatggcgg cgaaaccggc
     4921 gagaagggca ccgaatgcga agtggtcgcg cctggcgcgc atgacggtta ggaggaacca
     4981 gagtaggacg acggagatcc agaccatgaa tacggttgtg tagaggcgta actcggtcag
     5041 gccgaactct tgctggtaga ggtacatccg ctgcagcgcg gaggccacga cgacgaaaag
     5101 caggactacc attatcccgg caaacgcctt gaacagacgc tcgtgggcgc gggtctcggc
     5161 cctgagtagc cagtgggcga tgagcaagag ggggagtgcg agggcggtta cggtgaccag
     5221 ctcgaagaat ccgcggcggg cgtactcggc gtaggtcaga cccgctgtag cggccacgcg
     5281 ccccgcgcca ccgaacaggt agcggacctg gatcaccacg aaggtcagga aaagcaggtc
     5341 aagaagaccc agcactatcc ctacctcgac tatgcctagc gagagtaccc ttggacgcgg
     5401 gatcgcgaaa ttctcgggga cgcgcgccat cagccctgcc cagagcagtc ccgccgtgat
     5461 ccaggcgaag aaggtgatga gaaacaggtg cgagaagacc tcggcgaggt cgaagccgaa
     5521 gacctcgatt atgagccgtt cgaagatgga gtcggccgcc accaggagcg cgccgaagag
     5581 gagcaagagc ggggctgcga gcaggacgcc ccgcgacacc gccagcgcct gtcccttcca
     5641 tctcccacgg aacgtctcgc gccattcaat gtcggccacg ctcacgggga acggtcccgc
     5701 tgaggtcagg acgccggtat agacccctcc tagaacgtag tccaggatac cagcgcgcct
     5761 gaggctcccg ctgcgtccga gccacacggc aagggagagc gagatcagga cgccaaggag
     5821 atctaggaat accaccacgg gagaatcgcg caggacgacg ccggcggcga agacgagaga
     5881 aacgaagacc agccacttcc cctctccccc ggtgccaacg cgcccccatt tggcgagtaa
     5941 tacagtgagg ccgacaatgg ccattaccca gatgaggaag ttgatccccc agggcgtggc
     6001 gcgcaagagg acgtctccca ggccgccaag cgccaacgcg acgccgagaa tcccgagtcc
     6061 gagcctcgtc ctctctccca cagttgatac ctcccgtcct tggcctgaag ggcactgtag
     6121 cggacgggcg taacagatcg ggcaacggcc ggttaagaag aactaacctt tcctaagctc
     6181 ctcgtcccga ggataacgac ccgttacgat gacctactcc aggtggttac cgcaaacctg
     6241 gtcacccata gaggaatatc gcttcggtag aagtctgcta gtctacgacc tcgcgctgcg
     6301 gggctgtgcc aggacgcaac cacagcgaga cgtggaccgg ttgcatcgtc tctcttacca
     6361 cccctaccag gtcggcgttc aacgcctcca ggtctgtctc atcccgcagt ctggcggaga
     6421 actcttcgag cgtctttctt gcgtcgtact tcctcctgta gaaacggcgg tctatgaacg
     6481 actggttgcg acgacgcagc gggttgaaca gcgcagcgat caccagggta gagatgacta
     6541 tggcgagctg tggctgctgc tcctggccgg tgagcccgcg aaagatgacc tcggtcatcg
     6601 ccacgccccc gaagtacact agcgccagta tcacggtgag agaaccgtat acgagggtac
     6661 ggtttatgac cacatctatg tcgaagaggc gtgcgcgcag gactgcgatc ccgatggaga
     6721 gcgggatgaa cagcccgaat gcgtccatca gcagcatgct cagcatcttc gatcccactt
     6781 cctcgggcgg aaggtctacc gctgctccca cggtgaatat ggtcgtcagg gtgcccacta
     6841 tcgctacagc caccccgaac accacccatt tggtctgctg gcgctgggtc gcgtcggaga
     6901 cgcgtgcgta gcggtagagt tgggccacca ggaagctgcc ccacaccacc actacggccg
     6961 cattgaacag gaggggcggc cagctgggcg ggccgaaagg tgtgaagttc cacaaaccaa
     7021 agaaggcaac cccgtagagt agcgccatcc agagcgtcca gcgaggcacg aagcggccgc
     7081 taggaaagag gtagaaaaac agcatccacg ctatgtagcc aacaaacgtc accaggctgc
     7141 ctagcgtcgc ctgcaggaca gggtacatcc cctcggtcgc gctcggagta aggacgaaca
     7201 acccgttcag cgacccccag acaaccagca tcgtggcgac gagcagcgcc atagggtcgt
     7261 cggaacggcg ccagaagatg acgatggcga ccagagcgaa aaccgagacg aagacggtgt
     7321 caagggtaac gttgtacaga gcgtatccac ctgcggagag ccccatctcc gcgagggcgg
     7381 cacgcacccc cgcagtgggg tttgctagtt gggcgtagcg agcggggacg ctgattacaa
     7441 acgttcccag cgaaacgaag gccacggcca cccacgctgc cctcgcgaga accagcctgc
     7501 gcagggcagg gggccgcggc gcacgggggc tcatcccgcc tgcttctcga ccctgctgct
     7561 ttactccttc catcacgacc ccaccctaca gtaccatcgt cttccaagcg ttacggttac
     7621 cgttacgaag tcgaaaaaca cccctggcac ccattctcgc ccgagatacg ttcctctccc
     7681 agtagagccg acgcgttgac cttacgcgac cctggctcct ctcatactac cctcttcaaa
     7741 ggcggctcga aaagtccggg ggacgggatt tgcgcaccgg cttcgagaac tgtggagatc
     7801 ctctgccgga tctctggcgt atcgaagccc cgcagaccaa gagtcgttct gcggtaaatg
     7861 acgtcctctg cggtcagggc ccactcttcg tgaaccgcat ggtagatctg cgcccagagg
     7921 tccggcgctc ctggcattat tctttcgagc gcgttgtctt tcactttgct gtatccgagt
     7981 agcttacctg tctctgatcc gtacaggtgc agaagatggt ccaccttcga accgtcgagt
     8041 ctggtgtaga gatcccgaac aggagaggcg ccggggagag gtgcgtcgct tagggagagt
     8101 ctccggggcc tgactctagc aggcaggtgt cgcagcgcat cgagtgctat tcggcggtgg
     8161 gtggtcagct tgcctccggc caccgagacc atgccgcctg gccctacgct caggaggtga
     8221 ttccggctgg catggagcgt agtttcctcc cccatcggca agacgcgcag ccctgcgaaa
     8281 gcagagatga cacgctcgcg tcgcagcatc tgggggcgca ggaagcgcct ggccacttgc
     8341 aagagatagg agacgtcctc gggctccgca gctacagcgg cagggtcacc atggtactcc
     8401 tcctcggtag taccgagtag gatcatgtct tcgcagggca ccgcgtagag gtgctgtcct
     8461 ccttccaaag agacggcgac ggccgcgcgc cactgctcat ccggccgaag gacgacgtgg
     8521 attcctttgc tcaggcgggt gactggctcg caagcggaat cctccatgtt cctcaggaga
     8581 tccagccacg gacctgtggc gttgaccacg gccctgcacc tgacggtgat ctctacgccg
     8641 cgcccctgta gcgacacgcg tgagatccta cccggcgcca gatcgaggtt cacagcccgc
     8701 agataattcg ccaccacggc gccggaacgc gcggcggcgg tcacggtggc gagcgtcaga
     8761 cggctatcgt tggtcccggc ctcgaagtag acggcgtgtg aggcggtaac tcctacactg
     8821 agggggggca cgagaagggt ggcctcctcc ggggtgacga agcgcgggag cgggctcctg
     8881 aacccgtcta gcgctgtgta gacgagcagt ccggccgcgc acctcgagcg ctggcgccgt
     8941 cctccggccg cagataacac gaaaggtagc ggcctgacca gatgcggcgc gacgcgggag
     9001 gcgaagacgt cgcgctccct tagggccgta cgcacgagcc ggaaatcacc cgttccaagg
     9061 tagcgaagcc caccgtggac caggcgcgcg gaggctcctg aagt
//
LOCUS       DASTKM010000291        17059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_820247, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000291 DASTKM010000000
VERSION     DASTKM010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 17059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17059
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820247"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(391..519)
                     /locus_tag="VFQ10_11665"
     CDS             complement(391..519)
                     /locus_tag="VFQ10_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966044.1"
                     /translation="MIAPSLFTVGALDYPFEWGVRVGPEAFELALERFETSKLSGL"
     gene            725..1039
                     /locus_tag="VFQ10_11670"
     CDS             725..1039
                     /locus_tag="VFQ10_11670"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9966045.1"
                     /translation="MFARVSEWSVPPERQDEALRAAEEHIGPALRKQAGYCGDLLLGD
                     RQSGKMLTVTLWEDEQAMHATDEASHWFRSYGAWQTGGRAESTGHYEVYRAQDLGGTR
                     SS"
     gene            1149..1415
                     /locus_tag="VFQ10_11675"
     CDS             1149..1415
                     /locus_tag="VFQ10_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966046.1"
                     /translation="MSERDYLAKEETLRRLSEPEGDHELHTALDRMRVHHPHLWEVLH
                     RVHLAHDADPSKLEQWRRAPEGSQEDIWAEHYDKAMGVLQISLQ"
     gene            complement(1695..2225)
                     /locus_tag="VFQ10_11680"
     CDS             complement(1695..2225)
                     /locus_tag="VFQ10_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966047.1"
                     /translation="MKKSMVLLATVALLVALLASAGYAVRDTHVMKHWHPMFVEPALG
                     YFGLSFFGHDHTRHVHRVEDGAFVRAPITSCWNYDFLERDCDGPDHYKAFLADGSTRD
                     SMVGAYKGDPGSNSPPRAKACAVNTNSYREYCVSDYRDDGETVWLDTGRNLERHTLAI
                     GCPGPRLEYPCTSNDP"
     gene            2485..3765
                     /locus_tag="VFQ10_11685"
     CDS             2485..3765
                     /locus_tag="VFQ10_11685"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9966048.1"
                     /translation="MWDEEKNKALVRWWWEEVWVKGNVNAMDEFMAPNYVDHPNLPGL
                     SPGPEGMKQALSTYRTAFPELQATLDDIFAAGDRVALRWSARDTHLGDWLGVPPTGHH
                     FTMSGITIYRIAKGKAVEGWNSIEVNPTEEEQQWFTEGGGWPRRSGDIPAIERDPSPS
                     FWDVLTRNLTWYSRVREARERERIEQELQVARQIQRELLPEATPQLDGWEVTTYYEPA
                     REVGGDFYDFLEYEDGRLGLVVGDATGKGMPAALVMATTRGMLRAVVKSLGSPGEVLA
                     RVNDALVTDIPPNMFVTCLYAILDPKSGSLRYANAGHDLPYLRHHGGDAEELMARGLP
                     LGLMPNMRYEEKEAALAPGDNALFYSDGLVEAHDPQREMFGFARLRGLVAEHAEEGSL
                     VDFLMEELYSFVGEGWEQEDDITLLTLKRSASPS"
     gene            3890..4279
                     /locus_tag="VFQ10_11690"
     CDS             3890..4279
                     /locus_tag="VFQ10_11690"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9966049.1"
                     /translation="MIERTNAPALSQPPGYSHVVVASGSRLVTTAGAVPLDAEGNLVG
                     AGDLHAQARQTLQNLAIALEAVGARGRDVIKTTVYVVAAERGDLSSVWVAVRESGIAG
                     AASTLLGVSMLAIEGQLVEIEAMAVLE"
     gene            4371..5225
                     /locus_tag="VFQ10_11695"
     CDS             4371..5225
                     /locus_tag="VFQ10_11695"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HET9966050.1"
                     /translation="MNKLLRYCRAGYRGCIFGRDSELYIERGWSALETTLYVILTVFV
                     PVLMAVVGLVLVQRLVPPDRREEHNDVAGFIYAVLGVAYAVLLAFVVIVVWQDYKTAQ
                     TNVESEANELAGVYFLASRLPEPESTRLQELARTYARVVVEEEWPMMEQGQTSPRADS
                     LVRQLRSKLLEFDPRTRGEQVLYERGLTQLHDAVDARRSRLLQVREGIPNLLWVVLVV
                     GGVITVSFTYLFGLKSNVAHALIVAALTLVICGILFTIAEFNNPFSGPVEIRPDAFRE
                     VLHSFGGT"
     gene            5398..6126
                     /locus_tag="VFQ10_11700"
     CDS             5398..6126
                     /locus_tag="VFQ10_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9966051.1"
                     /translation="MVLLHGFLCDSRCWRPQLADLSDSFRVLAWDAPGAGSSSDPPDP
                     FTTTDWAQCLAGFLDVLGIERAQILGLSWGGILAQEFYRLYPDRVLTLILCDTYAGWK
                     GSLPRSACNKRLERCLRESSLPPKELVARWVPEFFTDAASHYLKEEMSAVVSEFHPLG
                     FRLMAKSSADTDTTDLLPNIGVPTLLVWGDDDQRSPMNIAEQFRDAIPNAELAIITSA
                     GHVSNMEQPGEFNAQVRRFCQSNL"
     gene            6452..6868
                     /locus_tag="VFQ10_11705"
     CDS             6452..6868
                     /locus_tag="VFQ10_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966052.1"
                     /translation="MDESYVRNELFAQAMEVGEFEVFPTPAELDGDLDNAFLISTGIL
                     LGVPKMFIVRVEEFHYSQEEGNQETDKILKEQERHIETVLSEHKIVLSEAAWNTVFSY
                     AVLDVHPSRTSGRLSEDEVRSVEEAFDALGLDPYRP"
     gene            7257..7544
                     /locus_tag="VFQ10_11710"
     CDS             7257..7544
                     /locus_tag="VFQ10_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966053.1"
                     /translation="MQIRALLRVTQRRQNLRAAPQRSLRQENHEGGHDLILANGYTND
                     RDIVKGGSGYDKIKVNDGDRWDTASGGAGGDWCIVDSISEAGTGCSRVSVR"
     gene            complement(7881..8354)
                     /locus_tag="VFQ10_11715"
     CDS             complement(7881..8354)
                     /locus_tag="VFQ10_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966054.1"
                     /translation="MSAEDPDSPKLPRLLSVPGLLAVAIFGISVIVVSRLFGPENILR
                     EIVTELLASFGSTILLFAIFGFFFRSGLRCLLRWAPGEETFEESVERFRELLQDFDVR
                     GRESEGSPDGEKLDRIEAGVRTLSADKIPRLENEIQELRKMLADPTDGRRDQTFE"
     gene            complement(8402..8977)
                     /locus_tag="VFQ10_11720"
     CDS             complement(8402..8977)
                     /locus_tag="VFQ10_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966055.1"
                     /translation="MNRLRLAELVSAATRLPVIAVPLFLAVGVEAAGTRGLLWAVLCI
                     FLTSGLSLLYLMYLTRSGKVSDPRAIPRVERVGPLRVVAALHAAAFVIVTLLGGPAEL
                     RAALLSYALATLGFVLVTPASNLSLHTAGVSGAAVCLAYVFGAWGLPAFALLPPVFWA
                     RLTLGRHTPVELALGAVVGGGGTWLSFHLIG"
     gene            9015..10082
                     /locus_tag="VFQ10_11725"
     CDS             9015..10082
                     /locus_tag="VFQ10_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET9966056.1"
                     /translation="MAQISGVIETIAPSSLAEEWDNCGLQVGGPEAEVNRVLVALTPL
                     PEVFEEAEEKGANFLLFHHPLIFDPLKAVVTSSYPGGLLARAIRNGLAVYAAHTSYDA
                     APAGVSVALARALGLSGPLRVLSPRGALRKLVVFVPEENADAVVKALAEVGAGVIGDY
                     TECTFRTLGRGTFRGGEGASPYLGEKGRLEEVEELRIETVVAAHTVGRAVNAATAAHP
                     YEEVALDVYPVEGSPDGCGYGRVGTLPERLTAEELREYVSESLGFPSRLLADPGRRVE
                     TVAVLGGSGGSFIPEVAASGAHAYVTGDVDYHEALLAESLDLAVIDAGHAATELPSLE
                     PLALRLTELVDVPVEVSRVLR"
     gene            complement(10174..11124)
                     /locus_tag="VFQ10_11730"
     CDS             complement(10174..11124)
                     /locus_tag="VFQ10_11730"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HET9966057.1"
                     /translation="MGKIVALGEVVSDIYRGEEISDVELGFVARPGGAPANVAVAACR
                     LGSEAAFVGSVGEDLFGDFILRALDSEGVDTSAVGRQSPPTRTSLAFVEIGADGDREF
                     TFYRSNPAADELLSPEDVAEDLVSGVSFVNFGSIPLIREPVRSATHRIAELAGKLDVP
                     VAFDVNFREHLWESAEAARDAVDPLLDLSTIVKLGDDELAPLLRTDDVQEAAGMLLDR
                     GVSLVLVSKGPEGAFYAARAFSGEVPAFDVGEIVDATGAGDAFLAAVLVHLSGKPLEE
                     DRVREATLMGCAAGALACTDYGAMRALPTKQELERFMESG"
     gene            complement(11161..12234)
                     /locus_tag="VFQ10_11735"
     CDS             complement(11161..12234)
                     /locus_tag="VFQ10_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HET9966058.1"
                     /translation="MDAYPIKLSYHVRDYYFGERLIPEMLGKRDAPEGVVAETWEISD
                     YRQTTGTVVNGSHAGRTLHDLVEEFPDELVGEGWRGPHFPLLIKFLDASNRLPVHLHA
                     DDQTAREKHGEPHGKAEAWHILWAAEDAKILAGVESDRSREELVGAFKAQDYESVMPQ
                     HKIRGGDTVYVPGGILHTFGPDTLILEVQQTSDLGQFVMPFDLYGNRLKEEEWDANIE
                     ATLDELKTDYLPRPNPGLVLEDAANRRLLCCAGPHFALERWVLTETHTDPSHPRRFLT
                     LSNVGEGPVELDYEGGSEILGRAESCVLPAAIGEVSVIPSRTGAATLVACYDPDLERD
                     VVGPLKEAGYSDEEIRALGEVGI"
     gene            12373..13251
                     /locus_tag="VFQ10_11740"
     CDS             12373..13251
                     /locus_tag="VFQ10_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9966059.1"
                     /translation="MAHYETGDARAERRRRVLLTGATGYIAGQLLPAFRERYDLRLID
                     VRETDASGNGVEGVEVFDLLSDEDSRLEPLFSDVDVVVHCGHFKPEGDDPETLYRGER
                     RNVDMMQRVYRLALEHGVERVVAASTNQAAKWYERPYYAGLRDRVGPEDYPRPDSFYG
                     WAKAAYEPLGFLYACGSLGRKLQVIQIRIVVPREIDATLFEDEPPERYVRELAGYISE
                     RDMQQLFCKSVETPDIEDEHGVPFHIFYGVSDNARKFWSITNARKVIRYEPTDDSEVR
                     FAEDIARLLHREELGA"
     gene            13248..13673
                     /locus_tag="VFQ10_11745"
     CDS             13248..13673
                     /locus_tag="VFQ10_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9966060.1"
                     /translation="MITDLGHTAFAAQDLDETIGFYGMLGIDEAFRLHHDDGSLMLVY
                     LHVSGDRFIEVFPGGPSPEPGREQSFMHICLLTDDLHAAVEHLRENGAPIDREPAVGR
                     DGNWQAWTRDPNGNEIELMQLSEESPQWQKARSAERGGT"
     gene            13690..16194
                     /locus_tag="VFQ10_11750"
     CDS             13690..16194
                     /locus_tag="VFQ10_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HET9966061.1"
                     /translation="MFGRARLKDFAPGEGVRAGAFQERFDEEAWIPISVPGDVHRALL
                     DAGRIENPFYDRNEKKCRWIEDREWWYRMSFHGPHEPLRPDERLCLVFHGLDTFATIY
                     LNGEELGRHHNMFREAVFDVGELVRPGENTIAIRFDRPLDHVGEEHPDQWGRNPERVF
                     MRKAQFGFGWDWGPRLPTVGIWRPVELRRERLASIRGVHLYTLDIDPTGKRAAVAVRV
                     EVERFAAYGPLEASVTLTPPDGVPAAESSLVFDGEDSILAAYLTVEKPRLWWTHDLGD
                     PFLYALRVDLQQEGDVLDNYESKVGIRTLELDQSPDPDEPGTRFFRFVLNGVPIFARG
                     ADWIPADSFVGAIEDERYETLISSARDANMNMLRVWGGGIYEHDIFYDLCNSMGLLVW
                     QDFMFACAVYPEEPPSFVSEIEAEAAYQVRRLRRHPCLALWCGNNENQWIHDHTFWDR
                     EDTTVPGALYYHEVLPRVVAEFDGRTPYWPGSPFGGDDHNDRRQGNVHNWEVWHGNFP
                     RHFGEESRRDPTPENVAYLRYAQDMGRFISEFGMHAAPVRETLRRAIPEGQLYHHSPS
                     MDHHNKDDPKNKGDDLMLTVTGLPTDLDEYVDFSMISQAEGLKFALEHFRRRKPHCSG
                     TLLWQLNDCWPVLSWSVLDYHGFGKAGYYYARRAYSPILASFKALPEGSIELWLTNDT
                     LKEVQDEIKVRLGTFEGETIWEENLQAHVPANASRPVARWYAERASGAPDRYLSVSSD
                     HGRFLSNRHFFAAIKDLRRAPVEPAVDITQFGEHELQVRLEAPAYAYFVHLEVPHEAT
                     RFSNNYFDLEPGQERTISVTNEKRSLKAENVTVRWR"
     gene            complement(16217..>17059)
                     /locus_tag="VFQ10_11755"
     CDS             complement(16217..>17059)
                     /locus_tag="VFQ10_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9966062.1"
                     /translation="SLVLAATFWGLNFAATKYAAESIPPLLLVALRFTVGGLLLLLVL
                     RLLEPKSRLRRSDLLPMAALGCFGVATAQTGFTFGVSLSSAGSTGLIFATAPVWGLLL
                     GAILGLERATWKGVLGVGLSIVGVALVVLDGLMSENASLVGDLLVLIAAFCVGAYAVL
                     SMPLLERHTPLEVATYPVLFGIPFLLLISSPQLLSLEWGSVGAGPWAAVAYSAVFATA
                     FAFSAWQWGISRIGANRVLVYQYLITVVGVTSGIVFFGETLGINKIAGGVVILLGVYL
                     ARRQ"
BASE COUNT         3395 a         5261 c         5302 g         3101 t
ORIGIN      
        1 ggacgaccct cgcgtgcagg ccaagaaggt cggcgccccc ggtgtgctga agctcgaact
       61 cggttcgggc ttttaccact ggaagttcgt ccccgtggtg ggcaggaact acaccgactc
      121 gggcagggcc cggtgccact aagtcatcgg aggaagcccc ttttgcaccc atctaaagcg
      181 tacttggtcc catattgaac agcacgtctg ctgaacttcg gagaactccg tcatggcgaa
      241 gtgcgtcgaa tcaaccttcc acacagttcg gtgaataaga gcaagaggag gaaccagcga
      301 cctagaaata gaagaccttg tgcgtagccg aagggctttt cccaccgcca ggcgactgac
      361 cacctggccc ccgcaatccg ctaggcttgc tcagaggccg ctgagcttgc tagtctcgaa
      421 cctctccagc gccagctcga aggcctcggg accgacccgc acgccccatt cgaacgggta
      481 atcgagggca ccgacggtga acagcgacgg ggcgatcacc gccgccaacg cccccaccat
      541 aagtgtgtac cagggtgttg tccaggccga agaggtaggt gaaccccacc acgatcacgc
      601 ccccgagaag caacactacc cacaggaggg ctgtgcgaac ttccgagaat caaacttgta
      661 cgcactcggg tggatcgcgg cataatgtga gtgccctcaa gagcggcgaa aggtggtggt
      721 ctgtgtgttt gcccgggtaa gcgagtggtc cgtaccccca gagcgccaag atgaggccct
      781 ccgcgcagcc gaagagcaca tcggtccggc gctcaggaag caggctggtt actgtggcga
      841 cttgcttctc ggcgacaggc agagcggtaa gatgctcacg gtaacgctgt gggaggacga
      901 gcaagcgatg cacgctaccg acgaggcttc gcactggttt cggtcttacg gcgcctggca
      961 gactggtggt agggccgaga gcacaggcca ctacgaagtc taccgtgccc aggacctcgg
     1021 gggtactagg agttcatgaa taggaaaccc ccgccgccag ttgcaccgct cctttgggat
     1081 aggagtacac gtgcgccaac gctcttgtgc tcggtatgat ggagcactcg aggaagggag
     1141 cagagaccat gagcgaacgc gactacctgg ccaaggagga gaccttgagg aggctctccg
     1201 agcccgaagg ggatcatgag ctgcacaccg ccttggaccg catgagggtc caccacccgc
     1261 acctttggga ggtgctccac agggtgcacc tcgcgcacga cgccgacccc tcaaagctgg
     1321 agcagtggcg aagggcaccc gagggtagcc aggaggacat ctgggctgag cattatgaca
     1381 aggccatggg ggtcttgcag atttcgcttc agtagcttca gaaaagcagg ttattacccg
     1441 taggcagacg gtctcagcca gggacgctta tgggcgttgg cccggggcta tgcacgatcc
     1501 tgcatccgga cttccgcgaa caccttctct gcgtctgccg tcccctgtcg cagttgagga
     1561 cttccggagt cagacgcacc gtatgggccc cgcaagccga acaataagac cccgagcacc
     1621 atccccgcaa ataccctcgc cagagctaga gcctccttct ccgttcctta ccgccagatc
     1681 ctacgtccgt cctctcaagg gtcgttgctc gtgcaggggt actcgagacg agggccggga
     1741 cagccgatag ccagcgtgtg cctttcaagg ttgcgtcccg tgtcgagcca cacggtctcc
     1801 ccatcatccc gataatccga cacgcagtat tcccggtagg agttggtgtt gacggcgcac
     1861 gccttggccc gtggtgggga gttggagcct ggatcgcctt tgtacgcgcc taccatagag
     1921 tcgcgggtgc tgccgtcggc caggaacgcc ttgtagtggt ccgggccgtc gcagtcgcgt
     1981 tcgagaaaat cgtagttcca gcagctcgtt atcggtgcac gcacgaaagc gccgtcctct
     2041 actctgtgca catggcgggt atggtcgtgc ccaaagaacg acagcccgaa gtagcccagg
     2101 gcaggctcca caaacatggg atgccagtgc ttcataacgt gggtgtccct gactgcgtaa
     2161 ccggcggagg cgagtaacgc tacaaggagc gccaccgtcg ccagcaacac cattgacttt
     2221 ttcacatccg tcctctcctc ggtccccctc tcctcagtag cttacgccac cagggacgcc
     2281 gctgtgcgcc ctactgcgga cctgggacca tcggaccggg gcccatgcac gatcccgcat
     2341 tcggacttcc gcgaacaccc ttccacgagg ttgggtgaat gagcctccag ccgatatgcc
     2401 gcatccctcc ttcgggtggc atcatgtgta tggtgcatag ccccggctag agaaaggcgc
     2461 gttggcaaag aggaggaagc tcccgtgtgg gacgaagaga agaacaaggc cctggtccgc
     2521 tggtggtggg aggaggtgtg ggtcaagggc aacgtgaacg ccatggatga gttcatggct
     2581 cccaactacg ttgatcatcc caaccttcct gggctatctc ctggccccga gggcatgaag
     2641 caggcgctca gcacctaccg caccgccttt cccgagctgc aggccaccct cgatgacatc
     2701 ttcgccgcag gagacagggt ggcgctccgc tggagcgctc gcgacactca cctgggcgac
     2761 tggttaggcg tccccccgac tggacaccac tttacgatga gcgggatcac catctaccgg
     2821 atcgccaagg gcaaggcggt ggaaggctgg aacagcatcg aggtgaaccc cacggaggaa
     2881 gaacagcagt ggtttacaga aggcggtgga tggccaagga ggagcggcga catccccgcc
     2941 atcgagcgtg atccgtcccc ctcgttctgg gacgtcctaa cccgcaacct cacctggtac
     3001 tcccgggtta gggaggcccg ggagcgggaa cgcatcgagc aagagctgca agtcgctcgc
     3061 caaatccaac gggagctcct tcccgaggcg accccccagc tcgacggctg ggaggtaacg
     3121 acttactacg agccggctcg ggaggtggga ggagacttct acgacttcct cgagtacgaa
     3181 gatgggcgcc tgggcctcgt cgtgggggac gccaccggca agggcatgcc ggcggcactg
     3241 gtgatggcaa cgacccgcgg catgcttagg gccgtcgtta agtcgttggg gtcgccgggc
     3301 gaggttttgg cacgggtcaa cgacgccttg gtcaccgaca tccccccgaa catgtttgtg
     3361 acctgcctct atgcgatcct cgaccccaag agtggtagtt tgaggtacgc caacgcaggc
     3421 cacgacctgc cctacctgcg gcaccacggc ggcgatgctg aggagctgat ggctagaggg
     3481 ttacccctcg gcctcatgcc gaacatgcgc tacgaggaga aggaggcagc gctcgcacca
     3541 ggcgacaacg ctctgttcta cagcgatggg ctggtcgagg cccacgaccc ccagcgtgag
     3601 atgttcggct ttgcgcggct tagaggtctg gtcgcagagc acgctgagga aggatcactg
     3661 gtggacttcc tgatggagga gctctactcc tttgtagggg agggctggga gcaggaggac
     3721 gacatcaccc tccttacgct caagcgttcc gcatccccaa gctgaagatc cgagaactct
     3781 ggcaaggcga agttcgcaga attctatttc cacgcactgg ggtgaatact gcccgcagac
     3841 agactacgat agctaccacg cgagagcttc gaaggtgaag gagcaggcca tgatcgaacg
     3901 cacgaatgca ccagcactaa gccagcctcc tggctactca cacgtcgtgg tcgcctcggg
     3961 aagccgactc gtcaccactg ctggggcggt ccctttagac gccgagggca acctcgtagg
     4021 agcaggcgat ctacacgccc aggctcgcca gacgctccag aacctagcga tcgccctgga
     4081 ggcggtcggc gccaggggac gcgacgtcat aaagacgacc gtctacgtgg tggcagctga
     4141 gcgtggggat ctgagttctg tgtgggtagc ggtgcgagaa tcgggcatag ctggggcggc
     4201 gagcacgctg ttgggagtgt ccatgctcgc catcgagggg caactcgtgg agatcgaggc
     4261 catggcggtg cttgagtagt ggcgcccaag ttgtagcgat gcggtcccag aaattcggag
     4321 aatgccgtta gggcgaagta gttcgcagaa ctctgcttct acgatgttgg gtgaataaac
     4381 tcctgaggta ttgccgcgcc ggttatagag gatgtatctt tggtcgagac agcgaactat
     4441 acatagagag aggctggtct gccttagaaa ccacactcta cgtcatcctg acggtcttcg
     4501 tgcctgtgct catggcggtc gtggggctgg tactggtgca acgtctggtg cccccggacc
     4561 gccgagagga gcacaacgac gtcgctggct tcatctacgc ggtcttgggc gtcgcctacg
     4621 ctgtgctgct tgcgttcgtg gtcatcgtcg tgtggcagga ctacaagacg gcccagacca
     4681 acgtggagag tgaggcaaac gagctggccg gggtctactt cctcgcctcc cgattgcccg
     4741 agcccgagag cacgcgcctc caggagctcg cccggacgta cgccagggtc gtggtggagg
     4801 aggagtggcc gatgatggag cagggccaga ctagcccgcg cgcggactcc ctcgttcgcc
     4861 agctcaggtc gaagctcctg gagttcgatc cccgcaccag aggcgagcag gtgctctacg
     4921 aacgtgggct gacgcagctc cacgatgcgg tcgacgccag gaggtcacgg ctgctccagg
     4981 tcagggaggg catacccaat ctgctgtggg tagtcctcgt ggtcggcgga gtgattacag
     5041 tgtccttcac ctatctattc gggctgaaga gcaacgtggc gcacgcgctt attgtcgctg
     5101 ccctgacgct ggtcatctgc ggcatactct ttaccatcgc cgagttcaac aaccccttct
     5161 ctgggcccgt cgagatccgg cctgatgcct tcagggaggt gctgcacagc ttcggaggga
     5221 cctgaataga aacccctccc ggcttttggc atgagggtca ttttcgctct ggcggatggt
     5281 tacttcgcgt tgagttccgc acggtggcat catactcaca tgagaagccc tgagttgact
     5341 cgcgccgagg tgaacggaca ttccgtggcc taccgagacg ctggggaggg tcctcctttg
     5401 gtactgctgc acgggttcct ctgtgattcc cgctgctgga gacctcaact tgcggatctc
     5461 tcggatagct tcagggtcct cgcgtgggat gcgccgggcg cggggtcctc ttccgatccg
     5521 ccggatccgt ttacgaccac ggattgggcg cagtgcctag cggggttcct cgacgtccta
     5581 ggcatcgaac gcgcgcagat cctcgggctg tcttggggtg gcattctcgc gcaggagttc
     5641 tatcgcctgt acccagatcg cgtgctgacg ctcatcctct gcgataccta cgctggatgg
     5701 aagggatcgc tgcctcgatc ggcatgcaac aagcgcctcg agaggtgttt acgggagtct
     5761 tcgctgcctc ccaaggagtt agtagcccgg tgggtacctg agttcttcac cgacgctgcc
     5821 tcgcattatc tcaaggaaga gatgtcggcc gtggtctcag agttccaccc gctcggcttt
     5881 cgcctgatgg cgaagtcctc agccgatacg gacaccactg atctgctgcc aaacataggg
     5941 gtgccgacgc tacttgtgtg gggcgatgat gatcaacgca gcccgatgaa catcgcagag
     6001 cagtttcggg acgccattcc caacgccgaa ctagcaatca tcacaagcgc tggacacgtg
     6061 agtaatatgg agcaaccggg ggagttcaat gcgcaagttc gccgtttctg ccagtcgaat
     6121 ctttagatgt aacctggcgc ggcgtctacc gaaagcctat tcacccgcgt gtgtggaagg
     6181 tgaattctct gaagttggac ttccgcgaag atggacttcc actctaccga attctgggaa
     6241 gatcgcacag ccccggtcca gtacccgtga gcggatcggg ctcagatcgg cttcgttcgg
     6301 gtatgatcta gatgaactct agactcactc agaatcgacc gccgtgcatc ggagctactc
     6361 cgcttgagag cagactagca atcacgtacc gaccgggccg gggtatgctg gcttcggtgg
     6421 ggtgttccgg cctttgactg agaggagcga gatggacgag agctacgttc gaaacgagtt
     6481 gtttgcccag gcgatggagg tgggtgagtt cgaagtcttc cccacaccag cggagctaga
     6541 cggggatctg gacaatgcct tcctgatctc taccgggatc ctcctcgggg ttcccaagat
     6601 gtttatcgtc cgggttgagg agtttcacta cagccaagag gagggcaacc aagagacaga
     6661 caagatactc aaagaacagg agcggcacat agaaacggtg ctctctgagc acaaaatcgt
     6721 gcttagcgaa gcagcctgga acacggtctt ctcctacgcc gtgttggatg ttcatccctc
     6781 taggacgagc gggcggcttt cagaggatga ggtgcgctcc gtagaggagg cgttcgacgc
     6841 cctaggatta gacccctaca gaccctagct gaagcgcact gagcagggcc ggcgtccact
     6901 aagggaaccc cgattttcgg acggggattc ttcctgtaac cgcgaggagt gctaagcggc
     6961 ctcgttacca gccaatcttt cccagcaggc ttcgggagat tgggcgccgt ttgcgacggg
     7021 gcttgtttgc cgcatgttgg gggtctatct ttcaggtatt gatccatctg ggggttgcgt
     7081 gttcctgtaa agggttgtta gtgttcgggt cacggcgtag gaggagggtt gtaagtcccc
     7141 atggagaaac agcagaggag atgatgcttg cgtaaattga tgatgttagg gactctggtg
     7201 gttatgctgg tgcccctggt cgccgccgcg gctttcgccg ccgaccagct gatcaattgc
     7261 aaatccgcgc cctgctacgg gtcacgcaac gacgacaaaa tctacgagcg gccccgcaac
     7321 ggagccttcg acaagaaaat catgaggggg gtcacgacct catacttgcc aacggataca
     7381 ccaacgaccg cgacatagtc aagggcggca gcggctacga caagatcaaa gtcaacgacg
     7441 gcgaccgctg ggatacggcc tctgggggcg cgggaggcga ctggtgcatc gtggactcca
     7501 tatccgaagc aggcacgggc tgctccaggg tgagcgtccg ctagagattc actgaaagca
     7561 gccgcttggg gagagccaag cctgcgccag cgtaacgcga cgacccggta ttccgtatgg
     7621 ggactgggct cgaaaggagc cccggtttct tggttggtac gggagcgtcc cgacaagcag
     7681 cgtgtggaag gtttggatct tcctgtaacc gcagcttcag gtttcaggat ctctgcgaac
     7741 agagctggcc cagcaggccg tgtactgggt agatggtcca accgatgtga agattgctgt
     7801 gaaggctacg tcctccacgt gagcttgtcc tgaacgctgg cggcgggtaa caaagcgcct
     7861 cggattcgtt agagactgcc tcactcgaag gtctgatctc tcctcccgtc tgtaggatct
     7921 gccagcatct tacgcagttc ctgtatctcg ttttccagcc ggggtatttt gtctgcggag
     7981 agggtcctta ctcctgcttc gatccggtcc agcttctctc catcagggga accttcagac
     8041 tccctccctc gaacatcgaa gtcctgcaga agctctctga agcgttccac ggattcctcg
     8101 aaggtctcct cgccgggtgc ccagcgaagc aagcaccgta ggccgcttct gaagaaaaac
     8161 ccgaagatgg cgaacaacag tatcgtgctg ccgaagctgg ccagcaactc cgtgacgatc
     8221 tccctcagga tgttctcggg accgaacagc cgcgacacca cgataaccga gatcccgaag
     8281 atggctacgg ccaatagccc ggggactgag agcaacctcg gcaacttagg ggaatcggga
     8341 tcttctgcgg acatctgatc tccctccacg gttcacccac actccctggc agcctaccgg
     8401 atcagccgat gagatgaaat gagagccagg tccccccgcc cccgacgacg gcgccgagcg
     8461 ccagttcgac cggcgtgtga cggcccagcg ttagccttgc ccagaacacg ggcggcagga
     8521 gcgcgaaggc cggcagtccc cacgcgccga aaacgtaagc caggcagacc gctgcgccgg
     8581 agacgccggc ggtgtggagg gacaggttgc tcgctggcgt gaccaacacg aagccaagcg
     8641 tggccagcgc gtaggagagg agcgcggccc tgagctccgc cggtccgccg agaagcgtga
     8701 cgataacgaa agccgcggcg tgcagcgcag cgactacacg caagggaccg acgcgctcga
     8761 cccgcgggat ggcccgcgga tcactgacct tccctgagcg ggtgagatac atcaggtaga
     8821 ggagcgagag cccgctggtc aggaagatgc agaggacagc ccagagcaga ccccgcgtcc
     8881 cggcggcctc gactccgacc gccaggaaga gcggaacggc gatgaccggc aggcgggttg
     8941 ccgctgagac gagttcggcc agcctgaggc ggttcacggt ccggagattg tacaatgcct
     9001 ggatcttggt gcgcgtggca cagatatcgg gagtgatcga aacgatagcc ccatcatccc
     9061 tggccgaaga gtgggacaac tgcggtttgc aggtcggggg cccggaggca gaggtgaacc
     9121 gggtcctggt cgcgctaacg ccgctgcccg aggtattcga ggaggcggag gagaagggtg
     9181 ctaacttctt gctcttccat caccccctca tcttcgatcc actcaaggcc gtggtcacct
     9241 catcctatcc tggaggcctc ctggccaggg ctatccgtaa cggtcttgca gtctacgcgg
     9301 cgcacactag ctacgacgcc gccccggcag gcgtgtctgt cgccctggca agggcgctcg
     9361 gcctcagcgg ccctctgcgt gtactgtcgc cgagaggagc ccttcggaag ctcgtggtct
     9421 tcgttccgga ggagaacgcg gacgccgtgg tgaaggcgct cgcggaggtt ggtgctggtg
     9481 tgatcgggga ctacacagaa tgcacgttca ggacgctggg aaggggcacc ttccgcggag
     9541 gagagggtgc gagcccttat ctaggggaga agggccggct ggaggaggtc gaggagttac
     9601 gcatcgagac agtcgtcgcc gcccacactg taggtcgtgc cgtgaatgcc gccaccgcag
     9661 cccatccata cgaagaggtg gcgctagacg tctaccctgt ggagggatct cctgatggct
     9721 gcggctacgg aagagtaggg actctgccgg agcggctaac ggcagaggag ctgcgcgaat
     9781 acgtctcgga gtcgttgggc ttcccttcgc gcctcctcgc agatccggga cgaagggtag
     9841 agaccgtcgc agttctcggg ggatccggcg gttcctttat ccctgaggtg gccgcctctg
     9901 gcgcgcatgc ctacgtgaca ggggacgtcg actaccacga ggcattgctt gcggagtcgc
     9961 tcgacctcgc cgtgatcgac gcaggacacg ccgccacgga gttaccttcg ctggagccgc
    10021 tcgccctgcg actaacggaa ctcgttgacg tgcccgtaga ggtcagccga gtgctgcgct
    10081 gaaacagggt gctgaaggca aactcccgca cctcgcgctg gtcagcaagt cagcatttcg
    10141 gtcctgggaa ggttctctcg tcgcggaact ctgtcaaccg gactccatga aacgctccag
    10201 ctcttgcttg gttggcaggg cgcgcatggc gccgtagtcc gtgcaggcga gggcgccggc
    10261 ggcgcagccc atcagggtcg cctcgcgcac ccggtcctct tccagcggtt tgcctgagag
    10321 gtgcacgagg acggcggcga ggaaagcgtc tcccgcgcct gtggcgtcca cgatctcgcc
    10381 cacgtcgaag gcgggcactt cgccgctgaa cgcccttgcg gcgtagaacg cgccttccgg
    10441 tcccttgctt acgagcacca gggaaacgcc gcgatccagg agcatcccgg cggcctcctg
    10501 gacgtcatcg gttctgagca gaggcgcgag ctcgtcgtcg cccagcttga cgatggtgga
    10561 gaggtccaga agcgggtcca cggcatctct tgccgcttcg gcgctctccc acaggtgctc
    10621 gcggaagttg acgtcgaagg ccacggggac gtcgagcttg cccgcgagtt ctgcgatgcg
    10681 gtgcgtagcc gagcggacgg gctcgcggat gagggggatg ctgccgaagt tcacgaaaga
    10741 cactcctgag accaaatcct ccgcaacgtc ctcgggcgag agtagctcgt cggcggcagg
    10801 gttggaccgg tagaaggtga actcacggtc cccgtcagcg cctatctcca caaaggccag
    10861 cgaggtgcgg gtcggcggac tctggcggcc gacagcggag gtatccacgc cttcggaatc
    10921 gagtgcccga aggataaagt ctccgaagag atcctcgcct acgctgccaa cgaaagcagc
    10981 ctcagagccg aggcggcagg cggcgaccgc gacgttcgca ggcgcacccc ccggcctggc
    11041 gacgaaaccc agctcgacgt cagagatctc ctctcccctg tagatatccg agacgacctc
    11101 gcccaaagcg actatcttcc ccaagacttc ctcccgaccc tactgctggt tctccaccgg
    11161 ctagattcct acctcgccaa gagcccttat ctcctcgtcc gagtatccag cctccttcag
    11221 gggccctacg acgtcgcgtt cgaggtcggg gtcgtaacat gccaccaggg tggccgcccc
    11281 ggtcctggag gggatcacac taacctctcc gatcgcggcc ggcaagacgc agctctcggc
    11341 ccgtccgagt atctcggaac cgccctcgta gtctagctcc accggccctt cgcccacgtt
    11401 cgagagcgtg aggaatcgcc gcgggtgaga agggtccgtg tgggtctctg tgagcaccca
    11461 gcgttcgagg gcgaagtgcg ggccggcgca gcacaggagg cgcctgttgg cagcgtcctc
    11521 gagcacgagt ccggggttcg gacggggcag gtagtccgtc ttcagctcgt cgagggtggc
    11581 ttctatgttc gcatcccatt cctcctcctt caggcggttg ccgtagaggt cgaaaggcat
    11641 cacgaactgg ccaaggtccg aggtctgctg gacctcgagg ataagggtgt ctggcccgaa
    11701 ggtgtgaagg atgccgcctg gcacgtagac ggtgtcgccg ccccggattt tgtgctgcgg
    11761 catgacggat tcgtagtctt gggccttgaa cgcaccgacg agctcctctc gcgagcggtc
    11821 actctcgacg cctgcgagta ttttcgcgtc ctcggccgcc cacaggatgt gccacgcctc
    11881 cgccttgccg tggggttcgc cgtgtttctc ccttgcggtt tggtcgtcag catgaagatg
    11941 gacgggcagc ctgttggagg cgtcgaggaa tttgatcagg agcgggaaat gcggcccgcg
    12001 ccagccctcc cccaccagct catcagggaa ctcctcgacg agatcgtgga gcgtgcgtcc
    12061 cgcgtgggaa ccgttgacga ccgttcccgt cgtctgtcgg tagtcgctga tctcccaggt
    12121 ctcggccacc acgccttctg gcgcgtccct cttgccgagc atctcgggga tcaaacgctc
    12181 cccgaagtaa taatcgcgca cgtggtaact caatttgatc gggtaggcgt ccacgcccat
    12241 ctccctgtcc cctcgtctcc aagcaggggt attctacact ggtcgagacg ctccctgaca
    12301 gcgccccgcg gtgcacggag ccggcgattg tgagtagata gggcggacgg cccccgacct
    12361 gggagaagac tcttggccca ctacgaaacg ggtgatgcgc gtgcagagcg gcgccggcgc
    12421 gtcctcctga caggcgccac tggctatata gcaggccaac tcctgccggc gttcagggag
    12481 cgctacgacc tgaggctcat cgacgtcagg gagacggacg cctcgggcaa tggcgtcgaa
    12541 ggagtcgaag tcttcgacct gctctcggac gaagactccc ggctggagcc attgttctcg
    12601 gatgtggacg tggttgtcca ttgcggacat ttcaagccgg aaggcgacga tcccgagacg
    12661 ctctacaggg gtgagcggcg caacgtggac atgatgcagc gcgtctacag gctcgcgttg
    12721 gagcatggcg tagagcgggt ggtcgcggcg agcaccaacc aggccgcgaa gtggtacgag
    12781 cgaccttact acgccggcct aagagaccgc gtcggtcccg aggactaccc tcggcctgac
    12841 agcttctacg gctgggccaa ggcggcctac gaaccgctcg gcttcctcta cgcttgcggc
    12901 agcctgggac gaaaactgca ggtcatccag atccgtatag tcgtgccgcg cgagatcgac
    12961 gcgaccctct tcgaggacga gcctcccgaa cgctacgtcc gcgagctagc aggctatatc
    13021 agcgagcgcg acatgcagca gctgttctgc aagtccgtag agacgccaga catagaagac
    13081 gaacacggcg ttcctttcca catcttctat ggcgtctcgg acaacgccag gaagttctgg
    13141 agcataacca acgccaggaa ggttatccgc tacgagccca ctgacgactc cgaagtccgc
    13201 ttcgccgaag atatcgcccg tctgctacat cgagaggagc taggagcttg atcaccgacc
    13261 tcggacacac tgccttcgcg gcccaggacc tggatgagac catcggcttc tacgggatgc
    13321 tcggcatcga tgaggctttc cggctgcatc atgacgatgg ctcgctgatg ctcgtatatc
    13381 ttcacgtctc gggagatcgt tttatagagg tctttcccgg cgggccttcc cccgagcctg
    13441 gcagggagca gagttttatg cacatctgtt tgctgaccga tgacctgcat gcggccgtcg
    13501 agcatctgcg ggagaacggc gcgcccatag accgcgagcc tgcagtcggc cgcgacggca
    13561 actggcaggc ctggacgcgg gaccctaacg gcaacgagat cgaactcatg cagctctcgg
    13621 aagaatcgcc gcaatggcag aaggctcgtt ccgctgaacg cggcgggacg taggagcggc
    13681 gtaaactcta tgttcggtcg cgcacggctc aaagactttg ctccgggtga gggggtcagg
    13741 gccggggctt tccaggaacg cttcgatgag gaggcctgga tccccatctc cgtaccaggg
    13801 gacgtgcacc gggctttgct ggacgcaggg cgcatcgaga atcctttcta cgaccgaaac
    13861 gaaaagaaat gtcgctggat cgaggaccgc gagtggtggt atcgcatgag tttccatggt
    13921 ccccacgaac cgctcaggcc agacgaacgg ctgtgcctcg tcttccacgg cctcgacacc
    13981 ttcgccacaa tctacctgaa cggcgaagag cttggccgcc accacaacat gttccgcgag
    14041 gccgtcttcg acgttggtga gctcgtacgc ccgggggaga acaccatagc catacgcttc
    14101 gaccgtccac tcgaccacgt cggagaggag caccccgacc agtggggaag gaaccccgag
    14161 cgggtcttca tgcgcaaagc gcagttcggc ttcgggtggg actggggacc gaggctcccg
    14221 accgtcggga tctggcgccc cgtagagttg cgccgcgaac gcctcgcctc catcagaggc
    14281 gtccacttgt acacgctgga tatagatcca acaggcaaac gtgccgcggt cgccgtacgt
    14341 gtcgaagtcg agcggttcgc ggcgtacggt cctctcgaag cttccgtcac gcttacacct
    14401 cctgacggag tcccagctgc agaatcttct ctcgtgttcg acggggaaga ttcgatcctc
    14461 gcagcctacc tgacggtgga gaaaccccgc ctgtggtgga cccacgacct cggcgatccc
    14521 ttcctgtacg ctctgagggt ggatctacag caggaaggcg acgtactcga caactacgag
    14581 tcgaaggtcg gcatacggac tctggagcta gaccagtccc ccgatcccga cgagcctggg
    14641 acgcgcttct tccggttcgt gttgaacggc gtaccgatct tcgcccgggg cgctgattgg
    14701 atccccgccg actccttcgt cggcgccatc gaagatgaac gctacgaaac cctgatctcc
    14761 tcagcccgcg atgccaacat gaacatgctg cgtgtgtggg gcgggggcat ctacgaacac
    14821 gacatcttct acgacctgtg caactccatg ggcctcctcg tctggcagga cttcatgttc
    14881 gcctgcgccg tctaccctga ggagccgccc tccttcgtct ccgaaatcga ggccgaggcc
    14941 gcctaccagg tgcgccgctt acgaaggcac ccttgcctcg ctctgtggtg tggcaacaac
    15001 gagaaccagt ggatccacga ccacaccttc tgggaccgcg aggacaccac cgtccccggc
    15061 gctctctact accacgaggt gttgccccgc gtcgtagccg agttcgacgg acgcactcct
    15121 tactggcctg gtagcccgtt cgggggagac gaccacaacg accgtcgtca gggcaacgtc
    15181 cacaactggg aggtctggca cggcaacttc ccccgccact tcggtgaaga gtcacgccgg
    15241 gaccctacgc cggagaatgt cgcttacctt cgctacgccc aggacatggg gaggtttata
    15301 agcgagtttg gtatgcacgc cgcgccagta cgcgagaccc ttcggcgcgc gataccggag
    15361 ggtcagctct atcatcacag cccctccatg gaccaccaca ataaagacga tcccaaaaac
    15421 aagggcgacg acctgatgct aaccgtgacc ggcttaccca cagacctgga cgagtacgtg
    15481 gacttcagca tgatctcgca ggccgaaggc ctgaagttcg ccctggagca cttccgccgc
    15541 cgcaaacccc actgctctgg taccctgctc tggcagctga acgactgctg gcccgtcctc
    15601 tcctggagcg tcctcgacta ccacggcttc ggcaaggccg gctactacta cgcgaggcgc
    15661 gcctactccc caatcctcgc ctccttcaaa gcgctacccg aaggctccat agagctctgg
    15721 ctcaccaacg acaccctcaa ggaagtgcaa gacgagatca aggtgcgact gggaacgttc
    15781 gaaggtgaga cgatctggga agagaatctc caggctcacg tcccggcaaa cgcaagtcgc
    15841 cccgtcgcac gctggtacgc tgagcgagca tccggcgccc ccgaccgcta cctttcggtg
    15901 agttcggacc acggccgctt cctctccaac cgccacttct tcgccgcaat caaggacctc
    15961 aggagagccc ccgtcgagcc ggccgtagac attacccagt tcggagagca cgaactgcaa
    16021 gtacgtctcg aggcacctgc atatgcctac ttcgtccatc tcgaagtccc tcacgaagct
    16081 acccgcttct cgaacaacta cttcgatctc gaaccaggac aagagcgtac gatctccgtc
    16141 accaacgaaa agagatcgct caaagcggag aacgtcacag tcagatggcg ctgatacctg
    16201 aaacctgttg ccttcgttac tgcctgcggg ccagatagac gccgagcagg atcacgaccc
    16261 caccggcgat cttattgatc cccagcgtct cgccgaagaa cacgatacct gacgtgaccc
    16321 cgacgacggt gatgaggtac tggtagacga gcaccctatt cgccccgatt cggcttatcc
    16381 cccactgcca agccgagaat gcgaacgcag tggcgaagac agcggagtag gcgactgcag
    16441 cccagggtcc cgctcccacg ctcccccact ccaggctcag tagctgtggg gaggagatga
    16501 gcagcaggaa ggggatcccg aacaaaacag gataggtcgc gacctcgagc ggggtgtggc
    16561 gttccagcag aggcatcgag agaaccgcgt atgcgccgac gcagaacgcg gcgatgagga
    16621 cgagcaagtc cccaacgagg ctggcattct ccgacataag cccgtccagg acgaccaggg
    16681 ctacccccac gatggagagc ccgacgccga gcacaccctt ccacgtcgcc cgctcgagac
    16741 cgaggatcgc gccaagcaat aaaccccaca ccggcgccgt cgcgaagata aggcccgtgc
    16801 ttccggcgct ggagaggcta acgccgaacg tgaaaccggt ctgggccgtc gccacaccga
    16861 agcaacccag tgccgccatc gggagcaaat cgctccttcg gagcctgctc ttcggttcga
    16921 gaagccgtag aacgagcagt aacaatagcc cgccgaccgt aaaccgcagg gcgaccagca
    16981 atagtggcgg gatactctcg gcagcatact tcgtcgcggc gaaattcagg ccccaaaagg
    17041 ttgcagcgag aaccagcga
//
LOCUS       DASTKM010000292         6503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1173967, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000292 DASTKM010000000
VERSION     DASTKM010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173967"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..751
                     /locus_tag="VFQ10_11760"
     CDS             383..751
                     /locus_tag="VFQ10_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET9966063.1"
                     /translation="MKEENLDFFKLVERALEGLPPELSRLLDNVAIVVDDWPDYSTPL
                     VAGGAEETLYGLYEGVPLTERGAGYYGFLPDKITIFRGPLERDFATGDLEEQVRITVV
                     HEIAHYFGFDEERLEELGWG"
     gene            772..1191
                     /locus_tag="VFQ10_11765"
     CDS             772..1191
                     /locus_tag="VFQ10_11765"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9966064.1"
                     /translation="MSIEQRMATPAEKVSGYISDFRNAREWMVGVESVERLGEDSYRL
                     VLESPIGKIEPEVRIIERGPESIRWVYTTSIEGGGRMEVSPDDGGGCVVSYTGEFHLK
                     RKLFDRAARLVGAEKFARANGERSLSRLKYLMEARRY"
     gene            1191..1364
                     /locus_tag="VFQ10_11770"
     CDS             1191..1364
                     /locus_tag="VFQ10_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9966065.1"
                     /translation="MPAGLLEILLIFIVFLVLGPRRIADLFRSLGTGVHDFIETLGTG
                     KDKEELPEKDDDK"
     gene            complement(1567..2490)
                     /locus_tag="VFQ10_11775"
     CDS             complement(1567..2490)
                     /locus_tag="VFQ10_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966066.1"
                     /translation="MEGLDGRETGGELRPDGEEASDTTEIGRGEIPDPKELMARLEIK
                     DNHIRELYEELTAFRLAADEARASKAAGEGHIEALERECARLKERVRDLEEETRDRRR
                     RREGSDRQIARLERELERKEGEISHRDYLLERQAEQMEADKQRAEELASRKDLALQDA
                     LRRVDGLERDLEEREDEISDLHSTIETLKGDLEAEQELRDRLADPANRLRAGIDLFNE
                     SGQRNAMNSLSRTMGQPEVHVELDAGDEPPVLLTFTWQGVTWQTYVSDPGPGVEEPRV
                     YLKSTGEDLSGVDRKPPNARVGHGDRVILGL"
     gene            complement(2496..3146)
                     /locus_tag="VFQ10_11780"
     CDS             complement(2496..3146)
                     /locus_tag="VFQ10_11780"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9966067.1"
                     /translation="MFTRAKTEPVPVVGAEAPEFNLPSAQGGQLRLSMRTPRGPVVVA
                     FYRPGDEEDVEYFKALAAKEEEINLAAGSVVAIGIAEPTEALNFARASGLKSYVLYDY
                     ARVTSRQWGLWEKDRRQGDYARRAVFIVGPDHKIAHAWIGERPEPEEILAKVSEISGL
                     PKPQEEPKEGESEEGDEKPKKMSAEEREKIKAERRAAREAGKSVKSEQPKTPKEEG"
     gene            3305..4852
                     /locus_tag="VFQ10_11785"
     CDS             3305..4852
                     /locus_tag="VFQ10_11785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9966068.1"
                     /translation="MRIIMTHGYMLSGTGSNIYVQSLCRALVGQGYTVHLLCQEQDPL
                     AYDFVGKSAKVDASGVEDLGEQETPYPGLCVVYRPEIGDLLPVYVYDDYPGWRVKTFL
                     DLTEEELDNYLRRNVEALKIVLGVSEAEAVITNHSVPGPLIARRALEGNDLPYVSIVH
                     GSCLQYVSRKSEKYMGLTREGLEDAREILALSSHSAGTIAENFPDLAGKTHSLPGGVD
                     TELFHPDAFDRGVLDHLQGGPGRGQAQRTSLREALESSGHAGELAIALRAVAASYDAR
                     SHDKDAGERLRGFLDRDGPLLVYVGKLIHSKGVHSLISAFSRVRKETGARLLVIGFGT
                     FREGLEALTYSLSAGDETTVERLAEMGRLLEGGPAGPLEHFELADEMLVDAEGMQDDV
                     LFVGALYHGDLTKVLPTADAAAVPSIFPETFGLVAAEFAASGVVPFVSDHSGLREAGE
                     IVGRSLPFDLRVGMDGLEENFAIALNDYLRLPEEERRRCEEVVRRNCVEHLSWGTLAE
                     KLVALVA"
     gene            4856..5155
                     /locus_tag="VFQ10_11790"
     CDS             4856..5155
                     /locus_tag="VFQ10_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9966069.1"
                     /translation="MDIEHILRDLGLILGAGLVSQLIATIIKIPQMVVLVAAGALIGP
                     SVLGIVSNPLDGVGAQLLFNIGVALILFHGGTGISLQVISRTAVGLGLLVCPAYS"
     gene            5188..6069
                     /locus_tag="VFQ10_11795"
     CDS             5188..6069
                     /locus_tag="VFQ10_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9966070.1"
                     /translation="MFGVGFPVALLVGAVLSSTDPAILIPLFDRLKVRPKVSQTVVAE
                     SAFNDVTGTVLTLTLVGAVEAGRFTFSGPALEFTKELVLGALIGAVAGAVLCYAVSST
                     ARAGIWDESPGVAILAVVALAYFSTESLGGSAYLAAFVMGLMVGNMELLGLGHHDEHA
                     TLLEGFLGQVAEIATLLVFVTLGLNLPFEALGEYFFGGLLVMFVFIFVARPITVLVCL
                     LPDRRGGWTGRETAFISWCREMGVIPAAVASLLLSRGVEGAEIAVSMVALAVCTTLLL
                     QATTAGYVARRLGLLDS"
     gene            6321..>6503
                     /locus_tag="VFQ10_11800"
     CDS             6321..>6503
                     /locus_tag="VFQ10_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966071.1"
                     /translation="MTQLTDSYDRKMDYLRISVTDRCNFRCQYCMPEDIKFQDKSHIL
                     TLEEMLTFAEACLELGV"
BASE COUNT         1084 a         2105 c         2033 g         1281 t
ORIGIN      
        1 tggacgcaga gcaggagcgc cttcagaggc tctatggtga gttccccgag gccagcgagc
       61 aggggcgatt cataaaacgt cagatccggg aggtggcggg acagtcgggc tcggtgaaga
      121 cctcggccat ggttcgccgc aaccagcgcg acaagacccc ttgaccctct gagtgtaggc
      181 tacctgagtc aataggttga cgaaccacac ccgctctccg cgaacaccaa gaggaactac
      241 cccacgaggg cgtcgacgtt cttggagtat ttggctgtga cgtttgggtg gggcaacgat
      301 acgccgatcg cgtatctgac caagaagccc acagagcata gacttcgcgg cgaggtctcg
      361 ccgctgaagt acaatgagtt tcgtgaaaga agagaacctg gatttcttca agctggtgga
      421 gcgggcgctc gaaggcctgc cgcccgagct ttcgaggctt ctggacaacg tggcgatcgt
      481 ggtcgacgac tggccagact actcgacgcc gctggtggcc gggggggcgg aagagaccct
      541 ctacgggctg tatgagggtg tgcctttgac ggagagagga gcgggctact acgggttctt
      601 gccggataag atcacgatct tccgcgggcc tctggagcgg gacttcgcta caggggattt
      661 ggaggagcag gtcaggatca ctgtggtaca cgagatcgca cactacttcg gcttcgacga
      721 ggagcgcctg gaggagttgg gttggggttg aagtcgggcc gaggcggcag cgtgagcatt
      781 gagcagcgga tggccacccc tgccgagaag gtctccggct acatatctga cttccgcaac
      841 gccagggagt ggatggtcgg cgtcgaaagc gtcgagcggc tgggggaaga ttcctaccgc
      901 ctcgtgctgg agagcccgat cggcaagata gaacccgagg tgcggataat cgagcgaggt
      961 ccggagagca tcagatgggt ctacaccaca tccatcgaag gcggcggccg gatggaggtc
     1021 tcacccgacg acggaggcgg atgcgtcgtc tcctacaccg gcgagttcca cctcaagcgc
     1081 aagctattcg accgcgccgc acgcttggtg ggcgctgaaa agttcgcccg tgccaacggg
     1141 gagcgctccc tctcgcgcct caaatacctg atggaagcca ggcgctacta gtgcccgccg
     1201 gacttctcga gatcctcctg atcttcatag tgttcctggt gctcggcccg cgccgcatcg
     1261 cagacctctt ccgctcgcta ggcacaggcg tccacgactt catagagacc ctgggcacgg
     1321 gcaaagacaa ggaagaactc cccgagaaag acgacgataa gtaaagcatt tcagcttttc
     1381 agccctttgg tgtcggtccg ccgcccttgc ttggccgcga atcgttcgct ttgtatatcg
     1441 cctcccgaga gaatcttccc tccgcaatcc atgagtccat gctgacttgc tgacaagcga
     1501 agcccgaagg gcgtagcgtg ccgacaaggc gcgaagcgcc gtgctggcta gctgaaatgc
     1561 tgaggcctac aggccgagta ttaccctatc gccgtgaccg acgcgggcgt ttggtggttt
     1621 gcggtccacg ccggagaggt cttcaccggt gctcttgagg tatacgcggg gctcttcgac
     1681 gccgggaccg gggtctgaga cgtatgtctg ccaggtaaca ccctgccagg tgaaggtgag
     1741 gagaacgggc ggctcatccc ctgcatccaa ctccacgtgt acttcgggct ggcccatggt
     1801 cctggagagg gagttcatcg cgtttcgctg cccggattcg ttgaacaggt ctatcccggc
     1861 ccgcagccgg ttcgcgggat ctgcgaggcg gtcgcgcagc tcctgctcgg cctccagatc
     1921 tccctttaac gtctcgatgg tcgagtgcag gtcggagatc tcatcctccc tctcctccag
     1981 gtccctctcc agcccatcga cccggcggag ggcgtcctgg agcgcgaggt ccttgcgcga
     2041 ggccagttcc tcggcccgct gcttgtcggc ctccatttgc tctgcctggc gctccagaag
     2101 gtagtcgcgg tgtgagatct ccccttcctt gcgttcgagt tcgcgctcca gtcgcgctat
     2161 ctggcggtct gagccctccc tgcggcgcct gcggtcacgc gtctcctcct cgagatccct
     2221 gacgcgctcc ttcaacctgg cgcactcgcg ctccagggcc tcgatgtgcc cctcccccgc
     2281 cgctttcgag gcgcgggcct catccgcggc gaggcggaag gctgtgagct cctcgtagag
     2341 ctctctgatg tggttgtcct ttatctccag ccgggccatc aactccttcg ggtctgggat
     2401 ctccccgcga cctatctccg tggtatcgga ggcctcttcc ccgtccggcc gaagctcgcc
     2461 gccggtctcg cgcccatcca ggccttccac gctccctacc cctcctcctt cggagtcttc
     2521 ggctgctcgc tcttgacaga tttgcccgct tcgcgggcgg cgcgacgctc cgcctttatc
     2581 ttctcccgtt cctcggccga catcttcttc ggcttctcgt cgccttcctc cgattcgccc
     2641 tctttcggtt cctcctgagg tttggggagg cctgagatct cgctgacctt cgccaggatc
     2701 tcctctggtt ctggcctctc tccgatccag gcgtgggcga ttttatggtc gggaccgacg
     2761 atgaagacgg cccgacgagc gtagtcgccc tgtctccgat ccttctccca gagcccccac
     2821 tggcggctcg taaccctggc gtaatcgtag agcacgtagg atttcagccc cgacgccctg
     2881 gcgaagttga gggcctctgt aggctcggcg attccgatgg cgaccaccga gccggcggcg
     2941 aggttgatct cctcctcttt cgcggccagc gccttgaagt actcgacgtc ctcttcgtcg
     3001 cccggcctgt agaaggcgac cacgaccggt ccccgcggtg tcctcatgct caacctgagc
     3061 tggcctccct gggcgcttgg caggttgaac tcgggagcct cggctcccac tacgggcacc
     3121 ggctcggtct tcgcgcgtgt aaacatgcgg tcctcctcgc gagtatgaag aatcataccc
     3181 gcgcatacta catcctagag aggctcttgg cgcgtatcgt atgcgggtct ggaacgcgac
     3241 tctgtgatac cttgcgctat gcgagcatgg agatgcgaac tcgacagggg ctatgaggag
     3301 cgccatgagg atcatcatga cccacgggta catgctctcc ggcacgggga gtaacatcta
     3361 cgtccagagc ctgtgtcgcg ccctggtcgg ccagggttac accgtgcatc tgctctgcca
     3421 ggagcaggac ccgctcgcct atgacttcgt cggcaagagc gcgaaggtgg acgcaagtgg
     3481 ggtcgaggat ctgggcgagc aggagacacc ctaccccggg ctctgcgtcg tctaccggcc
     3541 tgagatcggg gacctcctac ccgtctacgt ctacgacgac tacccgggat ggcgcgtgaa
     3601 gaccttcctg gacctgacgg aggaggaact ggataattac ctgaggcgca acgtcgaggc
     3661 cctgaagatc gtgctcgggg tgtcagaagc tgaggctgtg atcaccaacc actccgtgcc
     3721 agggccgctt atcgcacgcc gcgctttgga aggcaatgac cttccttacg tgagcatcgt
     3781 gcacggaagt tgcctccagt acgtctcccg caagagtgag aagtacatgg gtctcacccg
     3841 cgagggtctg gaagatgcgc gggagatcct ggccctctcc tcgcacagcg cgggcacgat
     3901 agcggagaac ttccccgacc tcgcgggcaa gacccactcc ctcccaggcg gggttgacac
     3961 cgaactcttc caccccgacg cgttcgaccg cggggtcctc gaccatctcc aaggcggacc
     4021 gggccgcggc caggcgcagc ggacgtcgtt gcgagaggcc ctggagagtt cgggccacgc
     4081 aggggagctg gccatcgcgc tgcgagcggt cgcggcctcc tacgacgccc gctcgcacga
     4141 caaagacgcc ggtgagcggc tccggggttt cctcgaccgc gacgggccgc tccttgtcta
     4201 cgtcggaaag ctcatccact ccaagggggt ccacagcctg atctctgcct tctcgagggt
     4261 gcgcaaggag acaggcgccc gcctgctggt gatcggcttc ggcacgttcc gcgagggcct
     4321 cgaggccctg acctattccc tcagcgcggg cgatgagacg accgtggagc ggctggccga
     4381 gatgggcaga ttactcgaag gaggaccggc tggtccgttg gagcacttcg agctcgccga
     4441 cgagatgctc gtcgacgccg aaggcatgca ggacgacgtg ctgttcgtcg gggcactcta
     4501 ccatggcgac ctgacgaagg tccttcccac cgcggacgct gcggcggtcc cctccatctt
     4561 ccccgagacc ttcgggctcg tagccgccga gttcgccgcg agcggcgtcg tccctttcgt
     4621 gtcggaccac tccggcctca gggaggccgg cgagatcgtg ggccgcagcc tacctttcga
     4681 cctccgcgtt ggcatggatg gcttggagga gaacttcgca atagctctca acgattacct
     4741 gaggctgcct gaggaagaaa ggcggcggtg cgaggaggtc gtgcgccgga attgcgtgga
     4801 gcatttgagc tgggggacac tggcggagaa gctcgtagcc ctggtcgcct agagcgtgga
     4861 catagagcac atcctccgcg acctcggcct catactgggt gctgggctgg tctcgcagct
     4921 catagcgacg atcataaaga tcccccagat ggtcgtgctg gtcgccgccg gcgccctgat
     4981 cgggccgtct gtgctcggga tcgtctcgaa ccccctggac ggggttggcg cccaactcct
     5041 gttcaacata ggcgtggccc tcatcctgtt ccacggcggc accggcatct cgctgcaggt
     5101 tatctccagg accgccgtgg gcctcggcct gctcgtctgc ccggcgtact cctgacagcc
     5161 ctcatcgtcg cgctggtggt ctcccccgtg ttcggcgtcg gcttccccgt ggccctgctc
     5221 gtaggagcgg tactgtcctc gacagatccc gccatcctca tccccctgtt cgaccggttg
     5281 aaagtccgcc ccaaagtgtc ccagacggta gtcgccgagt ccgccttcaa cgacgttacg
     5341 ggcacggtac ttacactgac cctggtcggc gccgtggagg ccggcaggtt caccttctcg
     5401 ggtcccgcgc tggagttcac caaagaactc gtgctggggg ccctgatcgg cgccgttgcc
     5461 ggagcggttc tctgctacgc tgtctcctcg acggcgcggg ccggcatttg ggacgagtcg
     5521 ccgggggtgg ctatcctcgc cgtcgtcgcg ctcgcttact tctcgaccga atctctcggc
     5581 gggtcggcct acctcgcggc cttcgtcatg ggactgatgg tcgggaacat ggagttgctc
     5641 ggcctcggtc accacgacga gcacgctaca ctcctcgaag gattcctcgg acaggttgcg
     5701 gagatagcca ccctgctcgt cttcgtcacg ctcgggctaa atctgccctt cgaggcgctc
     5761 ggcgaatact tcttcggcgg cctcctggtg atgttcgttt tcatcttcgt ggcccgcccc
     5821 ataaccgttc tggtgtgcct cctgcccgac cggcgcggcg gatggacggg cagggagacg
     5881 gcgtttatca gctggtgtag ggagatgggt gtgatccccg cagccgtcgc gagcttgctc
     5941 ctctcgcgcg gggtcgaggg cgcggagatc gccgtgtcca tggtcgccct cgctgtgtgc
     6001 accacactcc tcctgcaagc aacgacagca ggctacgtcg cccgccgtct cggcctgctt
     6061 gattcttagc acgccgccag gcttggccag gcggcttgtc agcatttcgg cacgtcgcct
     6121 gttccaccgt ccacgatcag ccggggcgtc gaaagcctcc tgggcaacac cttctcccgg
     6181 atcgtggcca cgcaaggttg ccaacgggcg ccgcggatcc tgggcagtat acccgcgggc
     6241 gattcggccg ctactctcga ctctgaaaga agatacaatc caataagagc tagtgatagg
     6301 cacaggcccg aaaggatgct ttgacacagc tcaccgacag ctacgaccgc aagatggatt
     6361 atctcaggat ctcggtcacg gaccgctgca acttccgctg ccagtactgc atgcctgagg
     6421 acatcaagtt ccaggacaag agccacatcc tgaccttgga ggagatgctc accttcgccg
     6481 aagcgtgcct cgagctaggc gtc
//
LOCUS       DASTKM010000293         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1175731, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000293 DASTKM010000000
VERSION     DASTKM010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175731"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFQ10_11805"
     CDS             <1..314
                     /locus_tag="VFQ10_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966072.1"
                     /translation="EFMDVLEVRGVEVGDQQMLRSISSVVPNAIAAFLLPLRDTTGKT
                     SHCSFAWTEGNPIVYLFRYIHFGEGDTPPVTHEVLREDEPRVATRDTRRRTMSSPRRA
                     G"
     gene            314..1639
                     /locus_tag="VFQ10_11810"
     CDS             314..1639
                     /locus_tag="VFQ10_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HET9966073.1"
                     /translation="MFPRLTAPRVIVLAFAGLIVAGTLLLKIPAANNGITWLDAFFES
                     VSAVTVTGLQAVVPADDFTPLGQGILAGLIQLGGLGIMTAATVGALLMGRQMGFRELL
                     IVREELASPDTPRNVARLIGQVALITFLVELVGVVALTVRFALEGHGIGSLGLGIFHS
                     VAAFCNAGFDIFEGGTATYAGDPTVNLILIYLVIAGGLGFPVLVNLYRYRKIRRLTLQ
                     SKLVLITTAVLLVLGILSVALLEWTNPRTLGGEPLGTKVLMSMFQGVTPRTAGFTTVT
                     YSDMQEPTLAVQIALMFIGTAPVSTGGGIKVTTLALIFLIFVSQIRGQDEVIAFERRI
                     PRSLIAKSLTVLSLSVLLVSGASLALMIFDEVSFLTALFEVTSAFGTVGLSLGPGYGL
                     ATELSPFGKLLLAAVMFAGRVGPITVVVALAQRSKPRRYTLPEEEIAIG"
     gene            1636..2304
                     /locus_tag="VFQ10_11815"
     CDS             1636..2304
                     /locus_tag="VFQ10_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET9966074.1"
                     /translation="MSRHFVIIGLGRLGVAMLDTLISLDHEVLGIDSDEDVIQELSGE
                     YPRAHLFAADATEESVLRDLDVGNFDGAAVVIGENMEAGILATANLKEIGVPFVVARA
                     ISKLHARVLEKIGADRIIEPERDMGAQVARTISSPAVIDYVDLGEDEALIEAEVPKRW
                     VGKSLADLHLSRESGLTILAVKPKGKSGTIPSGNTVLNEGDVIVVGGTKNALDRSPLV
                     RPDG"
     gene            2321..3655
                     /gene="trkA"
                     /locus_tag="VFQ10_11820"
     CDS             2321..3655
                     /gene="trkA"
                     /locus_tag="VFQ10_11820"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET9966075.1"
                     /translation="MHTVIIGAGEVGLNTARMLSHEGHDVVLVEMDEKLVEQASEELD
                     ALVIAGNGANPKLLNEAGIRNSDLLVAASNSDEVNIIACLAAKSQGVSRTVARIHNPD
                     YYEVGEPFAREMLGIDFIIHTEQMAAQEIKEALLVPGAINVDSFAEDTIEVAEVILNE
                     GSEAVGRALRDVRLPEGSLIVGVVRRGEALVPRGGTVLEMRDHVLLISGRRQISEAVG
                     ALATDTAPVRDVTIYGGGRIGLRLALSLEQAGMSVRVIERDEGRARYVASQLRKGFVL
                     HDEGISRDFLLQEGVDRTDAFVAVTGDDRANLLAAMYARQLGARMTIAGISRGEFAPL
                     ADALGVDLTISPRMLAAEAILRFVRKGEVIDVALLASGAEMIELRVPERCKVAGRPLS
                     EVGFPEGAIVGALLRNGKVIIPTGKEVLRPGDDAVVFTVEDAVEEVENLFTT"
     gene            3659..>4688
                     /locus_tag="VFQ10_11825"
     CDS             3659..>4688
                     /locus_tag="VFQ10_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HET9966076.1"
                     /translation="MNPRVIIRVNAGVILAIGLALLVPLALSLLYRDGSWPSFLLPAT
                     LMVAAGIVGFRLSRPRGRAPQYLSNRDVYLSVTLAWTLAALLEGTPFLVEGTFHSLLD
                     SSFEAMGGFTTTGATLLSNIEAQTPSILFWRSMTHWLGGIGIVVLFVAVAPLLGIGAA
                     RLIGAEMSGLTGPRLTPRIADTAKALVVIYLAISVAETAALLLARMPLYDAVVHTFGT
                     VATGGFSNKTASVGFYDSLAIEAIIGFFMVASGVSFSLYYLLYTRRRLDVLLDRELLA
                     YLGIIAAAAIFVWGILVLEGDYGDSWGRALRDSAFAVSSIITTTGFITADFDRWDAAA
                     QITLILLMF"
BASE COUNT          833 a         1459 c         1500 g          896 t
ORIGIN      
        1 aggagttcat ggacgtactc gaggtaaggg gagtcgaggt cggagatcag cagatgctgc
       61 gttccataag ctccgttgtc cccaacgcga tcgcggcctt cctgctaccc ctgcgcgaca
      121 caacggggaa gacgtcgcac tgctccttcg cgtggacgga gggcaaccct atcgtgtacc
      181 tctttcgcta catccacttt ggagaaggcg ataccccgcc cgtcacccac gaggtactgc
      241 gggaggacga accccgagtt gcgacccgcg atacacgtcg gcggacgatg agcagcccta
      301 ggcgtgccgg ctgatgttcc cccgactcac ggccccccgg gtcattgtgc tagccttcgc
      361 cgggctcatt gtggcgggaa ccttgttgct gaagataccc gcggccaaca acggcatcac
      421 ctggttggat gccttcttcg agtcggtgag cgcggtgacg gtcacgggcc tccaggccgt
      481 cgtccccgcc gacgatttca cgccgctcgg gcaggggatc ctagcaggcc tcatacagct
      541 cggcgggcta gggataatga ccgcggcaac agtaggcgcg ctcctcatgg gccgccagat
      601 ggggttccgc gagctcctca tcgtgcggga agagctcgca agccccgaca cgccgagaaa
      661 cgtcgcacgc ctcatcggcc aggtcgcgct cataaccttc ctcgtcgagc tcgttggcgt
      721 ggtggcactc actgttcgtt ttgcgctcga aggacacgga atcgggtccc tcgggctcgg
      781 gatcttccac tcagtagccg ccttctgcaa cgcgggcttc gacatcttcg agggtggaac
      841 cgcgacctac gccggagatc ctaccgtcaa tttgattctc atctacctgg tcatcgcagg
      901 cggcctcggg ttcccggtgc tcgtgaacct ctatcgctac cggaagatcc ggcgcctcac
      961 tctccagtcc aagctcgttc taatcactac cgccgtcctc ctggttctcg gcatcctgag
     1021 cgtcgcgctg ctagagtgga ccaacccccg tacactgggc ggcgagccct tagggaccaa
     1081 ggttctcatg tctatgttcc agggggtgac accgcggacc gccggattca ctacggtcac
     1141 gtactccgac atgcaggagc cgacgctcgc ggtgcagatc gcgctcatgt tcataggaac
     1201 cgcccctgtc tctacgggcg gggggatcaa ggtaacgacg cttgccctaa tcttcctgat
     1261 cttcgtctcc cagattcggg gccaggacga ggtgatagcc ttcgagcgaa ggataccgcg
     1321 atccctgatc gccaagagcc tcaccgtgct ctcgctctcg gtgctgctcg tctccggagc
     1381 gtccctggcg ttgatgatct tcgacgaggt ctccttcctt acggccctct tcgaggtcac
     1441 ctcggccttc gggactgttg ggctctcttt gggaccaggc tacgggctag ccacggagct
     1501 cagtccgttc ggtaaacttt tgctggcggc ggtaatgttc gctggacggg tgggaccgat
     1561 aaccgtcgtc gtggccctgg cccagcgaag taagccccga cggtacacgc tccctgaaga
     1621 ggagatagcg ataggatgag ccggcatttc gttatcatcg gcctggggcg gctcggcgtc
     1681 gccatgctgg ataccctgat ctccctcgac cacgaggtgc tcggaataga ctccgacgaa
     1741 gacgtgatac aggaactctc gggcgagtac ccgcgggccc acctgttcgc ggcggacgcc
     1801 accgaggagt cagtcttgag agacctggac gtaggcaact tcgacggcgc agcagtcgtg
     1861 atcggggaga acatggaggc cggcatacta gcaaccgcca acctcaagga gatcggggta
     1921 cccttcgtcg tcgcgcgcgc gatcagcaag ttgcacgcgc gggtgctgga gaagatcggg
     1981 gccgaccgca taatagagcc cgaacgggac atgggggcgc aggtagcccg gacgatttcc
     2041 tctcctgccg ttatcgacta cgtggacctc ggggaggacg aggccttgat agaagccgaa
     2101 gtgcccaaaa ggtgggtggg taagagcctc gccgacctgc acctctcccg cgagagcggc
     2161 ctgacgattc tggctgtcaa gccgaagggc aagtccggca ccataccgag cggcaataca
     2221 gtcctcaacg agggcgacgt gatcgtggtc gggggaacta agaatgccct ggaccggtcc
     2281 ccgctcgtcc ggcccgacgg ttagcaggag gtttcccgct ttgcacacgg tcatcatagg
     2341 agccggggag gtcgggctca acacggcgcg gatgctctcg cacgaaggcc acgacgtcgt
     2401 gctagtcgaa atggacgaga agcttgtaga acaggcgtct gaggagctag atgcgcttgt
     2461 aatcgccggc aacggagcca accccaagct gcttaacgag gctggtatta ggaactcaga
     2521 cctgctcgtt gcggcgagta actccgacga ggtgaacatc atcgcgtgtt tggcggccaa
     2581 gtcccaaggc gtctcccgca ccgtggcgcg tatacataat cccgactact acgaggtggg
     2641 agagccgttc gccagggaga tgcttgggat cgacttcata atccatacgg agcagatggc
     2701 tgctcaggag atcaaggaag cgctgctggt gcctggcgcg atcaacgtgg acagcttcgc
     2761 cgaggacacc atcgaggtgg ccgaggtgat cctgaacgaa ggctcggagg ccgttggccg
     2821 tgcactccgt gacgtgaggt tgccagaagg cagcctcatc gtcggcgtcg ttaggcgtgg
     2881 ggaggcgctc gtgccgagag gcggaacggt gctggagatg agggaccacg ttctcctgat
     2941 cagcggccgc aggcagatct ccgaggccgt gggcgctctg gccacggaca cggcgccggt
     3001 aagagatgtg accatctacg gcggaggacg catcgggttg cggctggccc tctcgctgga
     3061 gcaggcaggc atgtcggtga gggtcataga gagggatgag ggcagggcgc gctacgtagc
     3121 ttcacagctc aggaagggat tcgtgcttca cgacgagggg atctcccgtg acttcttgct
     3181 ccaggagggc gttgaccgta ccgacgcctt cgttgccgtc actggggacg acagggcaaa
     3241 cctcctcgcc gccatgtacg cccgccagtt gggggcccgt atgaccatcg ctggtataag
     3301 ccgcggcgag ttcgcgccgc tcgcggacgc cctcggggtc gacctgacga tctcaccgag
     3361 gatgctcgca gccgaggcca tactccgctt cgtgcgcaag ggcgaggtca ttgacgtggc
     3421 cctgctggca agtggagccg agatgatcga gctcagggtc cccgagcggt gcaaggtcgc
     3481 gggccgcccg ctctcggaag taggattccc cgagggcgcc atcgtggggg ctctactgcg
     3541 caacggaaaa gttatcatcc ccacgggcaa ggaggttctc aggccaggcg acgacgccgt
     3601 ggtgttcacc gtcgaagatg ccgtggaaga ggtcgagaac ctcttcacca cctagccttt
     3661 gaacccgagg gttatcatcc gggtgaacgc cggtgtgatc ctcgcgatag ggctggcgct
     3721 cctggtcccc ctcgcactct cgttgctcta ccgcgacggc tcgtggccga gcttcctgct
     3781 tcctgcaacc ctgatggtcg cagccggaat cgttggcttc cgtctctcca ggcctcgggg
     3841 ccgcgcgccc cagtacttgt cgaacaggga tgtctacctc tccgtaacgc tggcctggac
     3901 gctcgccgcc ctactcgagg gtacaccgtt tctcgtcgag ggtaccttcc acagcctcct
     3961 ggactcgagc tttgaggcga tgggcgggtt cacgaccaca ggggccacgc tactctcgaa
     4021 catcgaggcc cagacgccct ccatactgtt ctggcggagc atgacccact ggctcggggg
     4081 aatagggatc gtggtcctct tcgtcgcggt cgcgcctctg ctcgggatcg gcgcggcgcg
     4141 cctgatcggc gctgagatgt cgggtctcac ggggccccgc ctgacacccc ggatcgcgga
     4201 cacggccaag gccctcgtgg tcatctactt ggctatctca gtcgccgaga ccgctgcact
     4261 gctactggcg aggatgccgc tctacgacgc cgtggttcat acgttcggta ccgttgcgac
     4321 ggggggtttc agcaacaaga ccgcgtcggt cggcttctat gactcgctcg ccatagaggc
     4381 gataatcggc ttcttcatgg tggcatcggg cgtctccttc tcgctctact acctgcttta
     4441 cacccggcgc cggctcgacg tcttgctcga cagggagctg ctggcttacc tcgggatcat
     4501 cgcggcggct gcgatcttcg tgtgggggat cctcgtcctg gaaggcgact acggggactc
     4561 ctggggcagg gcgctcaggg actcggcgtt cgcggtatcc agcataatta ccacgacggg
     4621 attcataacg gctgacttcg accggtggga cgcggcagcc cagatcaccc tcatcttgct
     4681 catgttca
//
LOCUS       DASTKM010000294         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_296526, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000294 DASTKM010000000
VERSION     DASTKM010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296526"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..834)
                     /locus_tag="VFQ10_11830"
     CDS             complement(52..834)
                     /locus_tag="VFQ10_11830"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HET9966077.1"
                     /translation="MNEVSITSGPSVNVDLTGHSALVTGAASGIGRACAERLSRAGAA
                     VTVLDLEGDAARDVAEGIGGEPMQADLSDYEVLSGLEVDADIVVNNAGLQYVAAVEEF
                     PPERFSMILRIMLEAPFRIVQKALPGMYERGWGRVINISSIHGMVASPYKSAYVSAKH
                     GLEGFSKTVALEGGAKGVTSNCICPAYVRTPLVENQISDQASTHGISEDEVVEKIMLT
                     EPAIKRLIEPEEVAEFAAFLCTPEASFINGASLVMDGGATVH"
     gene            complement(1045..>2170)
                     /locus_tag="VFQ10_11835"
     CDS             complement(1045..>2170)
                     /locus_tag="VFQ10_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9966078.1"
                     /translation="AARPLGGIVFGHYGDRIGRKAMLILSLLIMGIATFLIGLLPTYQ
                     TIGVLAPILLVVLRFCQGIGVGGEWGGAVLMAVEHAPEGRRGFFGSWPQMGVPAGLLL
                     ANLVYFGSSTMLPGSWGWRVPFLLSIVLVGVGLFIRLRIMETPAFRQVKESGTEAPMP
                     ILDVLRTHPKNVLLAMGMRVAENGTFYVLTVFVLAYIEGELGMEQSTGLIGVIIAAAI
                     GLATIPLFGALSDRVGRRPVYLFGAAFSLLFAFPFFWLLNTGVAPLIWIAIVLGVNLG
                     HDSMYGPQAAYFSELFGTRVRYSGASLGYQLASVFAGGLAPLIATALLASFGWGAVAA
                     YTAAMALITVISVILASETYRGDLTATHTQERQLISEGGG"
BASE COUNT          431 a          709 c          661 g          369 t
ORIGIN      
        1 gggtcccttc cgcggtttcg gtcacgatag ttcctttctg ataagagctc tctagtggac
       61 cgtcgccccg ccgtccatga cgagggacgc gccgtttatg aacgacgcct caggcgtgca
      121 gaggaaggcg gcgaactctg ccacctcctc cggttcgatc agtcgcttta tcgcaggctc
      181 ggtgagcatt atcttctcga ccacctcgtc ttccgagatc ccgtgcgtac tcgcctggtc
      241 ggagatctgg ttctccacca gcggcgtcct gacgtaggcg gggcagatac agttggacgt
      301 cacgcccttg gcccctccct ccagggcaac ggtcttggag aagccctcca ggccgtgctt
      361 ggcggatacg taggcggact tgtaaggcga ggcgaccata ccgtggatgg aggagatgtt
      421 gatgacgcgc ccccagcccc gctcgtacat ccctggcaac gccttctgca cgatcctgaa
      481 aggcgcctcg agcatgatcc tgagtatcat cgagaagcgc tcgggcggga actcctcgac
      541 ggccgctacg tactgcagac ccgcgttgtt gaccactatg tcagcgtcga cctcgaggcc
      601 actgagcacc tcgtaatccg agaggtcggc ctgcatcggc tctccaccga tgccttcggc
      661 gacgtctctg gctgcatcgc cctcgaggtc gagcacggtg accgcagccc cggcccgcga
      721 gagcctctcg gcgcaagcac gccctatccc actcgccgct cccgtcacga gggcgctgtg
      781 tcctgtgagg tcaacgttga ccgacggtcc cgacgttata gagacctcat tcatgtccct
      841 ccttcacctc gaagaaacag cctgtcccag gcaggatctt tccccgatgc gctaccggga
      901 aaccagtata cagcgtcgtg cagagggctt tcgccttcta cacgacgagc tatcagcatt
      961 tcagcacttc agcaaacaac aagcccctgg aggcatgagg gacttccacc ttgcggccgc
     1021 tctggaactc ggggacgccc tttactagcc tcctccctcc gagatgagct ggcgttcctg
     1081 ggtgtgggtt gcggtgaggt cgcccctgta agtctccgag gcaaggatta ccgagatcac
     1141 ggtgatgagc gccatcgccg ccgtgtacgc cgcgaccgcc ccccagccga agctggcgag
     1201 caaggcggta gcaatgagcg gcgccaggcc gccggcgaag accgaggcca gctggtagcc
     1261 caaagaagct ccggagtagc gcaccctggt gccgaagagc tcggagaagt aggccgcctg
     1321 aggtccgtac attgagtcgt gaccgaggtt cacgccgagg acgatggcga tccagatcag
     1381 aggcgcaacg cccgtgttca acagccagaa gaatgggaaa gcgaagagca gcgagaaggc
     1441 ggccccgaac aggtacaccg gacgacgtcc tacgcggtcg gagagcgccc cgaaaagggg
     1501 gatggtagcg agcccgatgg cggcggcgat tatgacgccg atcaggcccg tgctctgctc
     1561 cattcccagc tcgccttcta tgtaggcgag cacgaagacg gtcagtacgt agaaggtccc
     1621 gttctcggcg acgcgcatcc ccatcgctag caagacattc ttcgggtggg tgcgcaacac
     1681 gtccagtatt ggcatgggag cctcggtgcc ggactctttg acttgcctga aagccggggt
     1741 ctccatgatc ctcagccgga taaagaggcc gacaccgacg agcacgatgc tgagcaggaa
     1801 cggcacgcgc cagccccacg acccgggcag catggtggag gagccgaagt agaccaggtt
     1861 ggcgagcagc agaccagccg ggacccccat ctggggccaa cttccgaaga agcctcgccg
     1921 gccctccggg gcgtgctcga ccgccatcag cacggccccg ccccactccc cgccgactcc
     1981 gatgccctga cagaaacgca ggacgacgag caagatcgga gccaggacac cgatggtttg
     2041 gtaggtcggc aacaacccta taaggaacgt cgcgataccc atgatgagga gcgacaggat
     2101 cagcattgcc ttgcgtccga tcctgtcccc gtagtgaccg aagactatgc cgcccagagg
     2161 cctcgccgcg
//
LOCUS       DASTKM010000295         5922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1001304, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000295 DASTKM010000000
VERSION     DASTKM010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001304"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFQ10_11840"
     CDS             <1..220
                     /locus_tag="VFQ10_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966079.1"
                     /translation="RTAPSTTPTRTTRRSWGRSMAIKVQSPGLLTTVQDTGRLGEYAI
                     GMPPSAIAKFLVENEEAVDTGQDLVEME"
     gene            321..509
                     /locus_tag="VFQ10_11845"
     CDS             321..509
                     /locus_tag="VFQ10_11845"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET9966080.1"
                     /translation="MGRRSTSGRQDKDEGSMDRAKGQMKEAAGALTGDKDTKAEGRAD
                     QRRGKVKEKKGNLKDLLK"
     gene            complement(662..1162)
                     /locus_tag="VFQ10_11850"
     CDS             complement(662..1162)
                     /locus_tag="VFQ10_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966081.1"
                     /translation="MDTTKRINRTAERLAKTSADSYKMVIDHMVAQQERNVRFAQEMF
                     GGTVREIRHQAESNRALTQELVERAEMQRDAFRTLVGESVDAYTDLLYAPFAYYKQGL
                     RLVESEGAGFPIAGYDALNVKEIGDRLDGMTAAEIRTVREYEKRNKNRETLIEQFDRK
                     LKAASA"
     gene            1479..2186
                     /locus_tag="VFQ10_11855"
     CDS             1479..2186
                     /locus_tag="VFQ10_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HET9966082.1"
                     /translation="MRLRNLVLLLAALTVMALAACSSSGQSSDEGGKDQAGKDASGEQ
                     AQKPPGKATEGKEEPPKMSKEAKARQVAPKKGEAQGQRKVSNALEPPKDKTLKLTIPE
                     MKQIEDDTIPTGMGTNEALFRDHAAVHLRDTGFPWQRTANVYIAGHRIGFPGTDSNLA
                     FYDLEDLKKGDKVYLEDAKGREYTYQVFGKVVVEPANLSVLRPIKGKNIVSLQTCTLP
                     DYTNRVIYRAVLKDVKS"
     gene            2322..3194
                     /locus_tag="VFQ10_11860"
     CDS             2322..3194
                     /locus_tag="VFQ10_11860"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET9966083.1"
                     /translation="MTALSKDDGPAFGAHAFIWAGEWTRECAERVISGAAEAGLDFVE
                     IPLLDPSSIDVERTRSLLDRYGLGYSCSLGLPAEIHLPSNPEGARRFLDGAVEATAAL
                     GSPVLTGCLYTHVGTLTGKPPEPSELQAVAHVLKRTARRAAEYGVELGIEAINRYETY
                     LVNLASQAGELLDRIDETNVFVHLDTYHMNIEEKGFYEPIVSLGSRLGYIHLSESDRG
                     TPGTANVHWDEVFRGLEDIDYGGALAMESFAAVNEALIGATALWRDVVGDPDALVRDG
                     LAFLRARGKEHGVL"
     gene            3239..4690
                     /gene="xylB"
                     /locus_tag="VFQ10_11865"
     CDS             3239..4690
                     /gene="xylB"
                     /locus_tag="VFQ10_11865"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HET9966084.1"
                     /translation="MLLGLDLGTSSLKALLLDEGGAPLGEGSAFYAVRAPRAGWAESS
                     PEDWWAAVLEATTAAVGPRGAEVKALGLSGQMHGVVLADELGLPLRSAVLWADARSGA
                     ELAAYRRLDEDLGRRLANPPAVGMAGPSLLWLRDQEPDAYTSARWALQPKDWLRMRLT
                     GEAATEPSDASATLLYDLLADDWAYAVAEDLGLRAELLAPLVPSACVAGTLEGEAAGE
                     LGLREGLPVAAGAADTAAAMLGTGLLRPGPVQLTVGTGGQVVTPRREPETDPHARTHL
                     YRAALPGLWYSMAAVQNAGLALEWVRKVLGVSWKDVYEEALAVPPGSGGVTFLPYLSG
                     ERTPRFDPGARGAWTGLGLDHTRGHLLRAALEGVAFALREALEALEDLGTIAPELRLA
                     GGGTGGDSGEPWRQLLADVLGRPLWRLPDEISSVASARGAAFLAGLASGIYPAAEDTL
                     PLTPEPESRILPGETTYEAAYERYTELYPRLYG"
     gene            4783..>5922
                     /locus_tag="VFQ10_11870"
     CDS             4783..>5922
                     /locus_tag="VFQ10_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HET9966085.1"
                     /translation="MSKEYSGPLWENGAAPGARWLLADRVSRTLSDARLESLADPVER
                     IARLAVPAGRVDPFRWLSEQRMFPKVYWSGREDRAGVAAVGFADLREAGVAGSLSKLL
                     ASLPDSGVSGARYYGGGRFDPLGQPDEEWAAFSAYRFVLPRFELHAGETETMLVCNLV
                     LPRDMDDASKIVQEIEDLSLPEGASRALLPEPIFREDSPDFQGWKENVERALEAFTEG
                     RLGKVVLARRAEFVFCRDLDPTLLLESLKAATPGCFHFYAEPEDGIAFLGASPERLFR
                     REGRSVESEAVAGTRPRGVSSAEDEGLRDDLLHSAKDRSEHTYVSKGIREALEPHCEE
                     LEIEDCVSEMMLARGRHLRSKVSGTLRDGVTDATLLDTMHPTPAVG"
BASE COUNT         1108 a         1789 c         1952 g         1073 t
ORIGIN      
        1 ccggaccgcg ccctcgacga cccccacgcg tacaacgagg agatcctggg ggcgctctat
       61 ggcgattaag gtgcagagcc ctggtctact caccaccgtc caggacacgg gccgcctcgg
      121 cgagtacgct atcgggatgc cccctagcgc catcgccaag tttctagtcg agaacgagga
      181 ggccgtggac acgggccagg atctcgtaga gatggaatag gatgctccct ggtttccggc
      241 gccaaaggta ccccaaggcg tttgattcca gcatctccgg gtaagtaaag tagcgaacat
      301 tcccgggcga ggaggaacag gtgggtagac gaagcacgag cggccggcag gacaaagacg
      361 aaggctccat ggacagggcc aagggtcaga tgaaggaagc ggccggcgcc ctgaccggtg
      421 acaaagacac gaaagccgag ggccgcgcgg accaacgccg cggaaaggtc aaggagaaga
      481 agggcaatct gaaagacctg ttgaagtagg gcttcaggcc gcagctagaa gaagtctata
      541 gacaggcgaa aggccgagga ttctttcccc ccggccttct cacttatcag ccgcgtttgt
      601 gtcagtatag tgttggcccg ttttagaacc cgacggctga aacctgttgc ctgagatctg
      661 tctaagcaga agcggccttg agcttgcggt cgaactgctc gatcagggtc tcacggttct
      721 tgttccgctt ctcgtactcg cggactgtgc ggatctccgc cgcggtcatg ccgtcgaggc
      781 ggtcaccgat ctccttgacg ttcaacgcgt cgtagccagc gatcggaaac cctgctcctt
      841 cgctctcgac gaggcgcaga ccctgcttgt agtaggcaaa gggcgcgtat agcaaatccg
      901 tgtaagcgtc aactgactcg ccaaccagcg tccggaaagc gtcgcgctgc atctcggccc
      961 gctcgacgag ctcctgggtg agcgccctgt tgctctctgc ctggtgcctg atctcacgca
     1021 ccgtgccgcc gaacatctcc tgcgcgaacc tcacgttgcg ctcctgctgc gctaccatat
     1081 ggtctatgac catcttgtag gagtcggcgc tcgtcttcgc cagccgctcc gcagtcctgt
     1141 tgatccgctt cgtagtatcc actctcttcc tcctctaatg gacatcgagt atgtgcagta
     1201 tacacatatg ttcaggcatg tcaaggttga tatccctcgc agcaagaatc ggttgtgact
     1261 gtggctccag ttcctggcct ttacacccgg ggttacaaac cttaatatga tcgtccggcg
     1321 aagtgatcca ggcgtggcgg aaaatgtggc catggtgtga gagatctctt gcggtgaggc
     1381 ttgtaacgga tgtttggtcc aaatgttgcg gcccggttat cctggatgta tatgctttct
     1441 gagcacggcg tctggtggag acagcgagag gtgatgagat gcgtttgaga aatctggtgc
     1501 tgttattagc ggccctgacg gtgatggcgc tggctgcttg ctcctcctcg ggccaatcct
     1561 ccgatgaggg ggggaaggac caggctggga aagatgcctc cggtgagcag gcgcagaaac
     1621 cgccagggaa ggcgacggag ggcaaggagg agcctcccaa gatgtcgaag gaggcaaagg
     1681 ccaggcaggt cgcgccgaag aagggtgagg cacaagggca gcgtaaggta agcaacgcgc
     1741 tcgagccccc caaggacaag accctcaagc tcacgattcc cgagatgaag cagatcgagg
     1801 acgacacgat accgacaggt atgggcacca acgaggcgct gttccgcgac cacgccgctg
     1861 tccatctgag ggatacgggt ttcccgtggc agaggactgc caacgtctac atcgcgggac
     1921 accgcatcgg gttccctggg acggacagca acctggcctt ctacgatctc gaggacctga
     1981 aaaagggcga caaggtatac ctcgaggacg cgaaaggcag agagtacact taccaggtct
     2041 tcggcaaggt cgtcgtcgag ccagccaatc tctccgtact gaggccgatc aagggcaaga
     2101 acatcgtcag cctgcagacc tgcacgttgc ctgactacac aaaccgtgtc atctacaggg
     2161 ccgtgctcaa ggacgtcaag tcgtaagcga cggacaacgg tgaatacgga gcgctaaaag
     2221 agccgtagct cccgggctgt gatccgcagg gtgacggcaa cattcagtgg ggacgcaggc
     2281 ccgctgcctg tgtgctaccg gcatcagaga acggagtaga gatgacggcg ctttcaaagg
     2341 atgacggccc ggccttcggg gcccacgctt ttatctgggc tggtgagtgg acgcgcgagt
     2401 gtgccgaacg tgtgatctcg ggcgccgccg aggctggcct ggatttcgtg gagataccac
     2461 tcctcgatcc cagttcgata gacgtcgagc gtacccgctc cctgttggat cgctacggcc
     2521 tcggttactc gtgctcgctc ggccttcccg cggagatcca tctgccatcc aaccccgaag
     2581 gcgcacggcg cttcctcgac ggggctgtgg aggcgactgc agccctgggc tcgccggtgc
     2641 tcacgggttg tctgtacacg cacgtgggca cgctgacagg caagcctccc gagccttcgg
     2701 agctccaggc cgtcgcccat gtcctcaaga ggactgcccg tcgcgctgcg gagtatggcg
     2761 tcgagcttgg tatcgaggcc ataaatcgct acgagacata tctcgtcaac cttgcttcgc
     2821 aggccggcga gctgctggat cggatagacg agaccaacgt cttcgtccac ctcgacacgt
     2881 accacatgaa catagaagag aagggcttct acgagccgat cgtctctttg ggatcacggc
     2941 ttggctacat ccacctctca gagtccgacc gtggcacgcc agggacggcc aacgttcact
     3001 gggacgaggt gttccgcggc ctcgaggaca tcgactacgg cggcgcgctc gcgatggaat
     3061 ccttcgccgc cgtgaacgag gcgctcatcg gcgcgacggc gctgtggcgc gacgtggtcg
     3121 gggatcccga cgccctcgtt cgggacgggc tcgccttcct gcgcgccagg ggaaaagagc
     3181 atggtgtgct ctgatgaagg agaaccgtct gtcccaggga cagggataag actccccgat
     3241 gctcctcgga ctcgacctag ggacgagttc cctcaaagcg ctgctactgg acgaaggcgg
     3301 cgctcctctc ggggagggct ccgctttcta tgcggtgcgt gcgccgcgtg cggggtgggc
     3361 tgagtcctct ccggaagact ggtgggcggc cgttctggaa gcaacgaccg ctgccgtggg
     3421 cccgcgcgga gcggaggtga aggccctcgg gctctccggg cagatgcacg gcgttgtcct
     3481 cgctgacgag cttggccttc ccctgcggtc cgccgtactc tgggcggacg ccagatccgg
     3541 cgcagagctg gcagcctacc ggaggctcga tgaggatctg ggccgccggc tcgcaaaccc
     3601 accggccgtg ggcatggcag ggccgagcct cctgtggttg cgagatcaag agcctgacgc
     3661 gtatacatcc gcaaggtggg cgttgcaacc gaaggattgg ctgcgcatgc gtctgacggg
     3721 ggaggccgct acagaaccat cggatgcctc ggccactctg ctctacgatc tcctcgccga
     3781 cgattgggcc tacgcggtgg ccgaggacct cggcctccgt gcagagcttc tggccccgct
     3841 ggtcccctcc gcctgcgtcg ccgggacgct agagggagag gccgccggcg aactcgggtt
     3901 gcgggagggg ctgcctgtgg cggcaggagc ggcagacacc gcggccgcca tgctcggcac
     3961 agggctgttg cggccaggtc ccgtgcaact cacggtcggc acgggaggcc aggtcgtgac
     4021 gccgaggagg gagcctgaaa cggacccgca tgctcgcacc cacctgtatc gggccgcctt
     4081 accaggcctc tggtactcga tggcggcggt ccagaacgca gggctagctc tcgagtgggt
     4141 ccgcaaagtg ctaggcgtct cctggaagga tgtctacgaa gaggccctcg cggtaccccc
     4201 aggctcagga ggcgtcacgt tcctgcctta cctgagcgga gagaggacac cgcgcttcga
     4261 ccctggcgcc cgtggcgcct ggaccggcct cggcctcgac cacacgcgcg gccatctcct
     4321 gcgcgcggcc ctcgaaggcg tcgcattcgc cctgcgtgag gcgctcgagg cgttggagga
     4381 tctagggacc atcgcccccg aactgcgcct cgccggaggt gggaccggag gagactcggg
     4441 cgagccctgg cgtcaactac tggccgacgt gcttggtcgc ccgttgtggc ggttgcccga
     4501 tgagatatcc tccgtggcgt cagcccgtgg cgccgctttc ctggccggcc tcgcctcagg
     4561 tatctacccc gcggccgaag atacgctccc tctcaccccc gagcccgaga gccgcattct
     4621 gcccggcgag acgacctacg aggccgccta cgaacggtac accgaactct accccaggct
     4681 ctacggttga gtgcctggcc tgcgactgtg agagaagccg ctaaaccact agatactcct
     4741 tgctacacta caccctgaga tgacgaggtg gcatagatct tgttgagcaa ggaatactcc
     4801 ggcccccttt gggagaacgg tgcggcaccc ggcgcccgct ggttgctggc cgaccgggtg
     4861 agccgtacgc tctcagatgc aagactggaa agcctcgccg acccggtcga gcgaatcgcg
     4921 cgtctggccg tacccgcggg ccgggtagat cctttcaggt ggctctcaga gcagcggatg
     4981 ttcccgaagg tctactggtc gggacgtgaa gaccgagccg gggtcgcggc cgtggggttc
     5041 gcggacctgc gggaggcggg cgtcgctggc tctctgtcca agctgctcgc gtcgctgccc
     5101 gactcagggg tttcgggcgc gcgttactac ggcggagggc gcttcgatcc gcttgggcag
     5161 cccgatgagg aatgggctgc gttcagcgct taccggttcg tcctgccgcg tttcgagctg
     5221 cacgccgggg agacggagac gatgctggtc tgcaatctgg tgctccccag ggatatggat
     5281 gacgcctcga agatagtaca ggagatcgaa gacctctcat tgcctgaggg tgcgtccaga
     5341 gctctgttgc cggaaccgat attccgtgag gacagccccg actttcaggg ttggaaggag
     5401 aacgtcgagc gggctctcga ggcatttacg gaaggtcgtc tggggaaggt cgtgctggcc
     5461 cgcagggctg agttcgtctt ctgtagggat ctggatccca cgctgctact ggagagcctg
     5521 aaggcggcta cgcctggctg cttccatttc tacgccgagc ccgaggatgg gatagcattt
     5581 ctcggggcct cgcccgagcg tctcttccgc cgtgaaggac gctcggtgga gagcgaggct
     5641 gtggcgggga ccaggccccg cggagtatct tcggcggagg acgaagggct gcgcgacgac
     5701 ctgttgcaca gcgctaaaga ccgctccgag catacttacg tgagcaaagg tataagggag
     5761 gcgcttgagc cgcactgtga agagctggag atagaggatt gtgtctctga gatgatgctg
     5821 gcgcggggga ggcacctcag gtcgaaggta agcggaacgc tcagggatgg cgtgacggac
     5881 gccacactgc tcgacacaat gcatcctacg ccagccgtcg gc
//
LOCUS       DASTKM010000296         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1002619, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000296 DASTKM010000000
VERSION     DASTKM010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002619"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFQ10_11875"
     CDS             complement(<1..245)
                     /locus_tag="VFQ10_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966086.1"
                     /translation="MSVVLNRGEKGRHSPRRGWTLLAFVSHTVRMQLRGVIIWGAALG
                     LYSAAMVASFTAIEGSADQLNQLMEAYPKGMLEAFGVT"
     gene            complement(242..1147)
                     /locus_tag="VFQ10_11880"
     CDS             complement(242..1147)
                     /locus_tag="VFQ10_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9966087.1"
                     /translation="MSLAIETEKLTKAYGRKARGIEDVDLAVEEGEVFGFLGPNGAGK
                     TTTVRTLLGFLRPTSGRGQVFGLDIRKDSVEIRARVGNLPGEFALEDKMTGEGLLKFF
                     ARLRGVEDLGYAHEISERLGAELHRPMRRLSRGNKQKIGLVQAMFHRPPLLMLDEPTG
                     GLDPLVQEEFLDIIEEVRDEGRTVFFSSHNLAEVERVCDRVGIIRGGRLAAVETTETL
                     IDKSFRHVVLTFAEDVDKEPFEALSGVRDLKGDGKRISFTLYDDLDKMVKLAARHRLV
                     NMEYERPSLEEVFLTYYERENGGVR"
     gene            complement(1220..1918)
                     /locus_tag="VFQ10_11885"
     CDS             complement(1220..1918)
                     /locus_tag="VFQ10_11885"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET9966088.1"
                     /translation="MTFTGLFRAWATVLVAMLAAILLTTAAFATGAGAQEQPPCSGKV
                     VLDPGHGGTDSGAVNTKYRLTEKEQTLDVANKLKALLEEDGCTVYMTRTDDRYLTNTQ
                     RANKANSFKAKNPGDYVLVSIHMNGSTSPSTDYTTTLFGKWRKDKELANTVFGSLSTL
                     PAANGTGTIATRKPYSFASGVLLKSDMPATIAETVFITSDREGQLLSNDTGARQQQIA
                     QAFRVGIEDYLTTH"
     gene            complement(1948..>2251)
                     /locus_tag="VFQ10_11890"
     CDS             complement(1948..>2251)
                     /locus_tag="VFQ10_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966089.1"
                     /translation="RTRQIEEIRQERDAARMEIDRLWSEIERLNLVEFQRELQEKTIS
                     TLEEEKATLQSERERLLKEQMDLTEEKSALSEDRVRLEAELKAMKNRGFWSRLFGG"
BASE COUNT          370 a          719 c          658 g          504 t
ORIGIN      
        1 gttacgccga aagcctcgag catccccttc gggtaggcct ccatcagttg gttcagctga
       61 tccgcgctcc cttcgatagc cgtgaacgag gctaccatcg cggcgctgta gagcccgagc
      121 gccgctcccc agatgatcac accccgcaac tgcatcctga ccgtgtgcga gacgaatgcc
      181 aacagcgtcc acccgcgcct tgggctatgc cttcccttct cgcctcgatt cagaaccacg
      241 ctcatcgcac ccctccgttc tcccgctcgt agtaggtgag gaagacctcc tcgaggctgg
      301 gacgctcgta ctccatgttc acgagcctgt gcctggctgc cagcttgacc atcttgtcga
      361 ggtcgtcgta gagggtgaag gagatcctct tcccatcacc cttgagatcc ctgacgccgg
      421 agagcgcctc gaagggttct ttgtcgacgt cttctgcaaa cgtaaggact acgtgcctga
      481 aagacttgtc tatgagcgtc tccgtcgtct cgaccgcagc gagcctgccg ccgcggatga
      541 tccccacccg gtcgcacacc cgctcgacct cggcaaggtt gtgcgaggag aagaagacgg
      601 ttcgtccctc gtccctgacc tcctcgatga tgtcgagaaa ctcctcctgc acgagcgggt
      661 cgagcccgcc ggtaggctcg tcgagcatca acaaaggcgg cctgtggaac atggcctgca
      721 cgagccctat cttctgcttg ttcccccgcg aaagcctgcg catgggacgg tgcaactcgg
      781 cccccagcct ctctgagatc tcgtgcgcgt agccaaggtc ctcgacgccg cgcagcctgg
      841 cgaagaattt gagaagcccc tctcccgtca tcttgtcctc cagggcgaac tccccgggca
      901 gattgccaac cctggccctt atctccacgc tgtctttcct gatgtcgagg ccgaagacct
      961 ggccacgccc actcgtcggc cgcaagaacc cgagcagggt ccttaccgtc gtggtcttgc
     1021 cggctccgtt gggcccgagg aaaccgaaga cctccccttc ctccacggcg agatccacat
     1081 cctctatgcc ccgcgctttg cggccgtagg ccttggtaag cttctcggtc tctatcgcca
     1141 ggctcatcag ttccttctat cctaccgctc caacacggca ggcaagtgat ggagcaagga
     1201 atatcgcctt ctccgtgtgc tagtgggtag tgaggtagtc ctcgatgccc accctgaagg
     1261 cttgcgctat ctgctgctgg cgcgctcccg tgtcgttgga gaggagctgt ccttctctat
     1321 cgctagtgat aaagacggtc tcggctatgg tcgctggcat gtcgctcttg aggaggacgc
     1381 ccgaggcgaa cgagtatggc ttcctggtgg ctattgttcc ggtgccgttg gccgcaggca
     1441 gggtcgagag cgagccgaag acggtgttgg ccagttcctt gtccttgcgc cacttcccga
     1501 agagggttgt cgtgtaatcg gtggagggac tagtggagcc gttcatgtgg atggagacca
     1561 gcacgtagtc cccgggattc ttagccttga aggagttggc cttgttggcg cgctgggtat
     1621 tggttaggta ccgatcatcg gtgcgcgtca tgtacaccgt gcagccgtct tcttcgagga
     1681 gggctttgag cttgtttgcg acgtccagag tctgttcctt ctcggtgaga cggtacttcg
     1741 tgtttaccgc tcccgagtcc gttccgccgt ggcccggatc caggaccacc tttcccgagc
     1801 atgggggctg ctcctgcgct cctgctcctg tggcgaaggc ggcagtcgtg agtaagatcg
     1861 cggcgagcat tgctaccagc accgtggccc acgccctgaa gagaccggta aacgtcatag
     1921 aagctccgtt ctttccgagt ggttcaatca gccgccgaag aggcgggacc agaagccgcg
     1981 gttcttcata gccttgagct cggcttcgag gcggacccgg tcttcagaga gggccgattt
     2041 ctcctccgtg aggtccatct gttccttgag gaggcgttcg cgctcggact gtagtgttgc
     2101 tttctcctcc tcgagggtcg agatcgtctt ctcctgcagt tcgcgctgga actcgacgag
     2161 gttgaggcgt tcgatctcgg accagaggcg gtcgatctcc atcctcgccg cgtcgcgctc
     2221 ctggcgaatc tcttctatct gacgggtccg c
//
LOCUS       DASTKM010000297         7950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_827904, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000297 DASTKM010000000
VERSION     DASTKM010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827904"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFQ10_11895"
     CDS             complement(<1..180)
                     /locus_tag="VFQ10_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9966090.1"
                     /translation="MDVARMSEAHVPEPGTIEGPSRQDLLRIYEQTGTIAVVGASADT
                     RKAAHVIPSYLQSQGY"
     gene            complement(663..884)
                     /locus_tag="VFQ10_11900"
                     /pseudo
     CDS             complement(663..884)
                     /locus_tag="VFQ10_11900"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
     gene            complement(1049..1357)
                     /locus_tag="VFQ10_11905"
     CDS             complement(1049..1357)
                     /locus_tag="VFQ10_11905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9966091.1"
                     /translation="MNAEVSNNPTEYRYELWADAELAGYTQYSLGRGRIAFVHTEIYE
                     SYEGMGLGTRLARAALDDARARGLVVVPYCPFIAHFIERHVDEYRDLVAPEMLTGDRQ
                     "
     gene            complement(1387..1794)
                     /locus_tag="VFQ10_11910"
     CDS             complement(1387..1794)
                     /locus_tag="VFQ10_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9966092.1"
                     /translation="MIDVGLLVLRLVLGVIFIGHGAQKLIGSFGGPRISGFAKMLEQL
                     GVKPAKPMAILAGLAEFVGGILAILGFLTPLAALALIGVMIVAVLTVHLKNGFFNTNG
                     GYEFNLALAGIAFTLLIVGAGAYSLDSVLGIFW"
     gene            complement(1791..2012)
                     /locus_tag="VFQ10_11915"
     CDS             complement(1791..2012)
                     /locus_tag="VFQ10_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9966093.1"
                     /translation="MADLEVGGLRHASSITKDAARNVGFYTGVLGMRLVYRSLTFDAP
                     SIHHLALANGASTGAARKGRPKIREGGSK"
     gene            2265..2657
                     /locus_tag="VFQ10_11920"
     CDS             2265..2657
                     /locus_tag="VFQ10_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9966094.1"
                     /translation="MAWLRMWRVVQKVKRAAAEHLRAYGLNYAQFDVLAHVGAAEGSR
                     QKELGESLHVTKGNVSHLVGQMERRGLVSRRQEGRTNRLYLTEEGRRLFEEVVPAHED
                     LVHQLMSALPEEEQARLHELLRSLDRSL"
     gene            complement(2919..3536)
                     /locus_tag="VFQ10_11925"
     CDS             complement(2919..3536)
                     /locus_tag="VFQ10_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9966095.1"
                     /translation="MRKLIVSNILSLDGYYEGPGKNVMALFDYRRDLYPIDESFDAYN
                     AERLRAADTLLLGRVTYDGFKSFWPSVADYPDATPIQREISRLNNAIDKVVISDTLTS
                     EETDPWHKSTRIISRAEAHEQIAELKGQVGRDVLVFGSRTLWNDLLANDLVDELHLMI
                     GPVVLGAGTPVFDEQPAVALRLIDTRMWDGSGNVLLRYEVRRQNT"
     gene            complement(3580..4335)
                     /locus_tag="VFQ10_11930"
     CDS             complement(3580..4335)
                     /locus_tag="VFQ10_11930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9966096.1"
                     /translation="MLLENKNAVIYGAGGAIGSAVAHAFAREGARVFLAGRTLESFEE
                     VAEGIRSAGGVAETAQVDAIDETAVDQHADTVAASAGGIDVSFNLISYGDIQGTPLAE
                     MPLEDFERPVMSALRTQFLTSRAAARHMVSQGSGVILFFGGYGDPLPDYYLGGFQVAL
                     NAIESLRRQLASELGAHGIRAVTLQTGGVPETIPESFDGREEITEGIVGQTMLGRAAT
                     LEDVGNVAAFAASDHARTMTATALNISCGAIVD"
     gene            complement(4936..5193)
                     /locus_tag="VFQ10_11935"
     CDS             complement(4936..5193)
                     /locus_tag="VFQ10_11935"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9966097.1"
                     /translation="MVAPDGAEYPNRGEYVEVDKPGRIVFTEPDTGITATITFAEVGD
                     KTEVTIHQVGVPERFLGPEAEAGFAAMFDKLEALLDQRTGG"
     gene            complement(5196..5387)
                     /locus_tag="VFQ10_11940"
     CDS             complement(5196..5387)
                     /locus_tag="VFQ10_11940"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9966098.1"
                     /translation="MADHIVDASQHYPEPGKVVDAPRDLVWQAFTEPEHLAAFWGGPG
                     ITVPVESVAMDVRLGAASS"
     gene            5423..5974
                     /locus_tag="VFQ10_11945"
     CDS             5423..5974
                     /locus_tag="VFQ10_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966099.1"
                     /translation="MKIAVVGRGNIGGGLAGLWEKAGHEVRRIGREGGDVSEAEAVLV
                     AVPGGAIAQSLDNVQGIDGKTVIDATNLVGVEPPSGFSSNAEYVKSRTNGPTAKAFNT
                     NFAALYDRIGEARSTPSNLWCGDEEARQVVEQLNRDAGYEPVYAGSLENAALQESFLK
                     LEFEINRGGLGRFFYRMAPPDQL"
     gene            complement(6032..7228)
                     /locus_tag="VFQ10_11950"
     CDS             complement(6032..7228)
                     /locus_tag="VFQ10_11950"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HET9966100.1"
                     /translation="MTTKAGVGMSRHHNPNLAGREAAEQALQKAGIVKPDFVVMFGSI
                     GYDQHSLVRAVREATGGAPLTGCSVEGTIDGDEADESNFSVLVTAISSDELRWHNGLA
                     TGLSADSCALGKRVAKDLLPHLSADTIGLFVFPDGLTYDIDNFFAGLEGDLPSERFLP
                     IWGGGAGNNLNLGKPTYQYCDDEVVSDAISYALLSGEARPAWAIGHSLIPIGGERIVT
                     RSQGNVLYEIDGKPATEVLKEYLPEDALTEDRDWLRYAISLALSFRAPSYMKDEEYVV
                     RGVPQLSLTDGSITVQTEVQEGTSIWFSSRDKEKITTGFDRMAAQIKEQLDGAQPELV
                     FQFECVTRGKLMFREQEKLQLLRRFRQSVGPEVPWVGFYTIGEIAPVEEHNGINRFAS
                     IILALS"
     gene            7704..>7950
                     /locus_tag="VFQ10_11955"
     CDS             7704..>7950
                     /locus_tag="VFQ10_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966101.1"
                     /translation="MGGLLRPEVARDPENVDRSVPPAHHKQSGHETERRSKAMDGWTL
                     SREYGSSQGAVRYEAFGTGPPVVLVHGTPFSSYVWRRV"
BASE COUNT         1535 a         2357 c         2410 g         1648 t
ORIGIN      
        1 gtatccctgc gactggagat agctcggtat gacgtgagcg gcctttcgcg tgtccgcaga
       61 ggctcccacg accgcgatcg tccccgtttg ctcgtagatg cgcaggaggt cctggcgtga
      121 tggaccttct atggttcccg gctcgggaac atgcgcttcg gacattctcg cgacatccat
      181 ccgtttcctc ctttgattgt gggtgacact cagagagtgg cccggaggcc ggctgcggac
      241 accgtgcgga agcactgttt ctggcctgta ccttcgtacg gtttacgcga agccatgctt
      301 cgaacctccg cgagaaaccg tcggaaaagc ctcggttcta tcgcgacaga gccccgcgga
      361 tctcaggcga cggcgaagac ggcggccagc accgcgacaa tggggatcat agtctccggt
      421 agggcggtgc cgacgcccgc gagcaccggg aaggctcatc gccgggcggt gatcgcgggt
      481 gagattagtt ctcccccgtg ccgagcgtgc cgccatgcca tttcacttac cgtgcgagga
      541 aatcggaagc gtgctcggtg tggtgttcgt tgaagactat ttgggagtag gtgcgcacga
      601 gttctttgtt agcttcgatg tcggtgttgt catgatgacg attcccccta gagtgtaggg
      661 ggctaatcca cgaacggtcc attgtagacg ggcgtctctc cgtacggctt cccggccatg
      721 agcatgaagc gcgtcccggg ctgcgcatcg acgatggtca gcgcgcgacc cggtccgaga
      781 actgcaatct gcgagcggct cgccaaccgc atgttcgcac cgaagctggc ctcgccttcc
      841 agcatgtaca cgaagccgtt gaagtcggac gggaccgggc tgctgaagac tccacccccg
      901 gcaactccac gtccaggatg agcccagggg tcccaagttc gaccggagag ccctccccta
      961 ccagcacccg cacgatcgcc tcaccttctt gcctgacggg cacgtcctgc ggatcgagga
     1021 gctgcgcgct agactctact cccttggctc attgtctgtc cccagtgagc atctcgggtg
     1081 ccacaaggtc acggtactca tcgacgtggc gctcgatgaa atgcgcgatg aacgggcagt
     1141 aaggcactac caccagtccc ctggcgcgcg cgtcgtcgag cgccgcccgg gccagccggg
     1201 tgcccagtcc catgccctcg taggactcgt agatctcggt gtggacgaaa gcgatccgcc
     1261 ctcggccaag gctgtactga gtgtagcctg ccaactcagc gtcggcccag agctcgtaac
     1321 gatactcggt tggattgttg ctgacctcgg cgttcatctc tcctccattc gagcgcaagg
     1381 tgtcgcctac cagaagatgc ccagaacact gtcgaggcta taggctcccg cacctactat
     1441 caggagcgta aaggctatcc ccgcaagcgc caggttgaac tcgtagcccc cgttggtgtt
     1501 gaagaagccg ttcttcaggt gcaccgtgag taccgcgacg atcatcaccc cgatgagggc
     1561 cagcgctgcc agaggcgtca ggaagcccaa tatcgccagt atcccgccga caaattccgc
     1621 gagtccggcc aggatcgcca tgggcttggc aggctttaca cccaactgct cgagcatctt
     1681 cgcgaagccc gagatccgcg gcccgccgaa gctaccgatg agcttctggg caccatggcc
     1741 tatgaagatc acacccagaa ccaacctcaa caccaacaac cctacgtcga tcatttcgaa
     1801 cctccctctc taatcttggg tcgtcccttc ctcgcggccc ctgtactcgc accgttggcc
     1861 agggccaagt ggtgaatcga aggggcgtcg aaggtcaggc tcctgtagac caggcgcatc
     1921 cccaacaccc cggtgtagaa ccccacgttc ctcgcggcat ccttggtgat cgaggacgcg
     1981 tggcgcaggc caccaacttc cagatccgcc atgtgcgacc tccttagttc aatgttgaac
     2041 tcacagatag tttaatattg aactttgaga tgtcaaccgc tgttgcgaaa gagtccactc
     2101 cggcacgctg gcggatatct cttgtggctc tggccggctt cgtaccgttc cggcggttcg
     2161 aactcgggtt gatggctggc tggtttaaca ttaaactccc tattacaatg ggagtggagc
     2221 gacaatccgt acgtcggggc ggtggcttga ggaggcactc ggtcttggcg tggctcagga
     2281 tgtggcgggt cgtccagaag gtgaaacggg cggcggccga gcacctaagg gcttacggct
     2341 tgaactacgc tcagttcgac gtgctggcgc atgtgggggc tgcggagggg tccaggcaga
     2401 aggagttggg tgaatcgctg cacgtcacca agggcaacgt cagccacctg gtgggccaga
     2461 tggagcggcg ggggctcgtc tcgcggcgcc aggaggggcg gaccaaccgt ctctacctca
     2521 cagaggaggg gcggcggctt ttcgaggagg tggtgccggc ccacgaggac ctcgtgcacc
     2581 agctgatgtc tgctcttccc gaagaggagc aagcgcggct ccacgagctg ctgcgaagtc
     2641 tggaccgctc cctgtagcag ccgcgcagaa gatcgctcgc ctgcagcacc gactgccgtg
     2701 gcaaaagcac ctcacccgcg cccgaagttg caggcggacg ccctgccagg tgaggtcagc
     2761 gcccgtgcgc tcgtagttgc tatgcactct tgaggtgaac ccctggccac ccttcgacta
     2821 attacgccaa taggtatgtt gtctatgttg ggggctgatc tggcggtggt ggaactggca
     2881 gcgtgaggga tagggcctag gatcaaagcg ccggcgatct acgtgttttg gcggcggact
     2941 tcgtaccgta ggagtacgtt ccctgagcca tcccacatgc gggtgtcgat aagccgaagg
     3001 gcaaccgctg gctgttcgtc gaataccggc gtgcccgcac ccaaaacaac cgggccgatc
     3061 atcaggtgta gttcgtcgac cagatcgttt gccagcaaat cgttccagag tgttcggcta
     3121 ccaaatacca agacgtctct gccgacttgg cctttgagtt cggcaatctg ctcgtgagcc
     3181 tctgcgcggc tgatgatgcg ggtgctctta tgccacgggt ccgtctcttc tgaggtcagg
     3241 gtatctgata tgaccacctt gtctatcgca ttattgagcc gtgatatctc cctttggatt
     3301 ggcgtggcgt cgggatagtc tgccacggac ggccagaaac tcttgaaacc atcgtatgta
     3361 acacgtccca gcagcagcgt atcggctgcc cgcagccgtt cggcgttata ggcatcgaag
     3421 ctttcgtcaa tcgggtagag atcccgacga tagtcgaaca gggccatgac attcttaccc
     3481 ggtccctcgt aatagccatc cagtgacagg atgttgctga cgattagttt acgcattaca
     3541 tacctcctca tgcctcgatc gagatgagag tatctgcgtc tagtctacga tcgccccaca
     3601 gctgatgttg agagctgtcg cagtcatggt gcgggcatga tctgaggcag cgaaggcggc
     3661 cacattgccg acatcctcaa gcgtcgctgc ccgccccagc atcgtctgcc caacgattcc
     3721 ttctgttatt tcctcgcgcc catcgaagct ctctgggatc gtctctggca ccccgcccgt
     3781 ctgcagcgtc acggcacgga taccatgcgc tccgagctcg gaggccaact ggcggcgcag
     3841 ggattcgatg gcgttgagtg cgacctggaa tcctcccagg tagtagtcag gcaacgggtc
     3901 cccgtagccg ccgaagaaga gaatcacccc agatccttga gagaccatgt gccgtgcagc
     3961 cgccctggag gtcagaaact gggttctcag ggcgctcatg accggccgct cgaagtcttc
     4021 gaggggcatc tcggcgagcg gtgtcccctg gatgtccccg taggagatga ggttgaagga
     4081 gacatcgatc cctccagcgc tcgcggccac agtgtcagcg tgttggtcga ccgccgtctc
     4141 gtcgatggca tcgacttgcg ccgtctcggc cactcctcca gcggagcgga tcccctcggc
     4201 tacctcctcg aagctctcaa gggtacgacc ggcgaggaac actctagccc cctcgcgggc
     4261 gaaggcgtgg gccaccgcgc taccgatcgc ccctcccgcc ccgtagatca cggcgttctt
     4321 gttctcgagt agcatcggcc gacctcctgt gattctgtgt cccaatcaac actcagcaga
     4381 ctgagtgtat cgtcgtgacg cgctccttct tatctcatct tcggagaata ctaccttgct
     4441 ggggatagct ccttttggtg gcggcgcgta tcgcattgct gctcagcctt tctagcgacg
     4501 aagctatccc cgaagtcgca gctacttcca agcgcataat tgggcttgca gcggatcggt
     4561 aggctggtag agacttggca atggagatct acgaatcttg ccgcgagcgc cctccaaggt
     4621 tggcccgccg aaggtcaggc aggaagagaa gtgaatcagc gctcttagga agagccgctg
     4681 ttcgccacat ctttcgccct gcccactgag tctttgagtc agtgtcccta ctcctgcccg
     4741 ttggctatgg tggtgccttt ggtaagcagg ttcatgggtc tagctcggcc ctcattgggg
     4801 tcacggtacc gaaatgggag ttttgtacgc taacgatcta cctttaacga gtctcagaag
     4861 tcaaagatcg ttcgtgaccc gttgagtaaa gtcttggagg tggaagggag caaaccaagc
     4921 agcgtggaga gtcgctcagc caccggtcct ctggtcgagg agggcttcga gcttgtcgaa
     4981 catggcggcg aatccggctt cggcttcggg tccgaggaac cgttcgggga cgccgacctg
     5041 gtggatcgtc acctcggtct tgtccccgac ctcggcgaag gttatcgtcg cggtgatccc
     5101 cgtgtcgggt tctgtaaaca cgatccggcc gggtttgtcg acctcgacgt actcgccgcg
     5161 gtttgggtat tcagcgccgt cgggggctac cattgtcagc tcgaagctgc ccccagacgg
     5221 acgtccatgg cgacggattc gaccggcacg gtgatccctg gtccgcccca gaacgccgcg
     5281 agatgctcgg gctcggtgaa ggcttgccac acgagatccc tgggagcgtc gaccacttta
     5341 cctggctcag gatagtgttg gctagcgtct acgatatgat cagccatgtg agctagccac
     5401 tgaagcgagg gaaaggatcg aattgaagat cgctgtggtc ggtagaggca acatcggtgg
     5461 ggggcttgcg ggtctctggg agaaggcggg ccacgaggta cggaggatcg gacgagaggg
     5521 tggcgacgtc tcggaggctg aggccgtact cgtggcggtc cctggcggcg cgatcgccca
     5581 gtccctcgac aatgtgcagg gcattgacgg gaaaacggtg atcgatgcga cgaacctcgt
     5641 gggcgtggag cccccctcgg gcttctcctc gaacgccgaa tacgtgaagt ccaggacgaa
     5701 cgggccgacg gccaaggcgt ttaacaccaa cttcgcagcg ctctacgata ggattggcga
     5761 agcgcgctcg acaccgagca atttgtggtg cggtgatgag gaggcgcggc aggttgtcga
     5821 gcagctcaac cgagatgcag gctacgagcc ggtgtatgca ggatctctcg agaacgccgc
     5881 gttgcaggag agcttcctca agctcgagtt tgagatcaac cggggagggc ttggccgatt
     5941 cttttatcgg atggcgcctc ctgatcaact ctgagacgat actcccgcat cctccgtcaa
     6001 acgcgttgcg ctagtatggt ccctttcggc gtcaactgag ggcaaggata atcgaggcga
     6061 atcgattaat gccattgtgc tcctctaccg gagcaatctc tcctatggtg taaaaaccca
     6121 cccatggcac ctccgggcca acggattgtc gaaaccgcct gagtagttgc aacttctcct
     6181 gctcacggaa catcaatttg cctctggtca cacactcaaa ctggaaaacg agttccggtt
     6241 gggcaccgtc tagttgctcc tttatctggg ctgccatccg atcgaagccg gtggtgatct
     6301 tctccttatc tcggctcgag aaccagatgc ttgtgccctc ctggacctcc gtttgtacgg
     6361 tgatggagcc gtcggtcagg ctcagttggg gtacgcctcg aacaacgtac tcctcatcct
     6421 tcatgtagct aggggctctg aaactgagcg cgagcgatat agcgtaacgc agccagtcgc
     6481 gatcctcagt gagggcatcc tctggtaaat actccttcag tacttcagtg gcgggtttac
     6541 cgtctatctc gtagaggacg ttgccctggg agcgtgtcac tatgcgctcg ccacctattg
     6601 ggatcaggct atggccgatg gcccaggcag ggcgcgcctc gcccgagagc agagcgtagg
     6661 agattgcgtc ggatacgacc tcgtcgtcgc aatattggta ggttggcttc cccaaattga
     6721 gattgttgcc ggctcccccg ccccagattg gcaggaaccg ctcgcttggg agatccccct
     6781 ccagccctgc aaagaagttg tcgatgtcat acgtgagccc atcaggaaag acaaaaagtc
     6841 caatggtgtc agcagaaagg tggggcagca ggtccttggc tacccgcttc ccaagggcgc
     6901 atgagtcggc actcagtccc gtcgccaatc cgttgtgcca gcgcagctcg tccgatgaga
     6961 tggccgtaac caacaccgag aaattagatt cgtccgcttc gtctccatct atcgtgccct
     7021 ctaccgaaca acctgtgagg ggtgcacctc cggtagcctc gcggacagca cgcaccaagg
     7081 agtgctgatc gtatccaata gagccgaaca tgacgacgaa gtctggcttg acgatgcccg
     7141 ccttctgtag tgcctgctca gcggcctcgc gaccagcgag gttgggattg tgatgtcgac
     7201 tcatgccgac gccagctttg gttgtcatga gcgtacctcc tgcttgtgag gaacatctta
     7261 gctcaggaga ggttgagcag catctcttga gacgccttat ctcttctcac cacagggcgg
     7321 attggtactc gtatggctat cctggttcga agccgtgtat gagggcacag tcgcagcgag
     7381 gcacgtgcct gtggcacgca tgtgggtcaa tgtcatcgca atccgctgtc agaatgcaaa
     7441 catccagacg ttcgctggca aaccttgcgg gcagaatggg cttcctccgg tgtagcgggt
     7501 actctggagt tgatgtggaa ggagcaagaa gtccatggag cataagaagc tgccccaacg
     7561 ccttgtccgc aacgtcggtg tgttctatgc ctgttaccgc ctacacccga tggtctggaa
     7621 tgcgacgcgc tacgccaggg ggcggaatgt cgttatcgat agtcaaggcg aggagagcag
     7681 tcatgccgct ggcagaaacc tgagtgggcg gcctcctccg ccccgaagtg gcccgtgacc
     7741 ccgagaacgt ggaccgaagt gtcccaccgg cccatcataa gcagagcggg cacgaaacgg
     7801 agaggagatc caaggcgatg gacggatgga cgttgagcag ggagtacggt tcgtctcaag
     7861 gcgcggtgcg ctacgaggca ttcgggacgg gacccccggt ggtgctggtc cacggcactc
     7921 ccttttcctc ctacgtgtgg cgcagggtcg
//
LOCUS       DASTKM010000298         6321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1180936, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000298 DASTKM010000000
VERSION     DASTKM010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180936"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="VFQ10_11960"
     CDS             <1..131
                     /locus_tag="VFQ10_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET9966102.1"
                     /translation="ASRFRRLAKDYERLPATLAGLHFVAFACLFLQQASGILGVGS"
     gene            complement(214..1656)
                     /locus_tag="VFQ10_11965"
     CDS             complement(214..1656)
                     /locus_tag="VFQ10_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET9966103.1"
                     /translation="MSGAGVAGASLLAGCERVGVLPDLPGNTPSADRDTNVVLIIMDS
                     LRKDHIGVYGNDKIKTPNLDALAEESLRFSRAYPESAPTICARRAIHTGLRTWPFEDW
                     RRYRGIDVGLQGWQPIPNDQITLAETMRSADFETLFVTDNLQQYDASMNFQRGFDAFD
                     FIRGQTTDSYRPLYTCPPGKVEQALVNKAAVGPGSRTYFQRYFANTAYRRSEEDWFAP
                     QVFTRASEFLETTRSERPFFLMVDCYDPHAPWDPPEEYVDMYDEGYDGPEPFAPVDGG
                     SEYLSGRQIRRMSALYSGEVTMTDRWLGRFLDKMEELDLSDNSLLIFLSDHGIAHGEH
                     GVVGKLPSTLWPEVTDIPFLIRHPEGKGAGATSDYFASTHDVAPTILGFLGIEPLQAL
                     DGQDLSVMLEGGEPEGRPHFTLGYHDHVFTRDEEYAMMSRNDGTDARLYDLREDPGMY
                     EDVAGDLPDIARRMFDDYVLEDAGGPLPRY"
     gene            1801..2082
                     /locus_tag="VFQ10_11970"
     CDS             1801..2082
                     /locus_tag="VFQ10_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966104.1"
                     /translation="MIQVKPVYNPGMTVFRYGREHFEEGEYVRRVEATPGDDLDPVVR
                     IVEDLGTVEIPCGKVDCRSNCLEYLVSATGRRNDYTLAECKISALVSII"
     gene            complement(2138..2710)
                     /locus_tag="VFQ10_11975"
     CDS             complement(2138..2710)
                     /locus_tag="VFQ10_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966105.1"
                     /translation="MPHRRRGGWIVILLRLLGIGAVYGVGFMFVKSLFPEPFVLLILQ
                     GGQSSEGDLTTLALVYMGVGLLSGLIAGPLFGGALLLRRGRSEGSGPRFVLSLALALL
                     TGFISGVLTLIVYATGILPGGGVLDPLRLIEGSNHPPGVPLLVAWTIARDLLPAGLAG
                     LFLSPLAGGVLQRLYAAERPPAQKGYSWEE"
     gene            2853..3986
                     /gene="queG"
                     /locus_tag="VFQ10_11980"
     CDS             2853..3986
                     /gene="queG"
                     /locus_tag="VFQ10_11980"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET9966106.1"
                     /translation="MRRVLEERALEAGFDLVGVARAEPLREGGERLREWQEAGMAADM
                     GYMHRPVELLSDPRKLQKSARSVVSLGVSYYPGDHPENEVGGRVARYAWGRDYHEVIK
                     GRLFRLREDLEEELGVRIRARGFTDAVPLLERSAAQHAGLGFFGRNSCLINNETGSYF
                     FIADLIVDLDLDPDPPGKGTCGRCTRCMDRCPTGAIKAPGVVDARLCISYLTIENRGE
                     IPRQLRPKVGDWAFGCDVCQEVCPYNKTKASRSRWPEFSEEAGSGPYLEIEKVLEIRT
                     DEAFEDRFGGTPLTRPSRAGLLRNCCVAAGNLKLEKAVPALVRALRGDPSPLVRGHAA
                     WALGEIGGAAESLMEASETEKDTWCLQEIRLALTNGTTEATLG"
     gene            3999..4823
                     /locus_tag="VFQ10_11985"
     CDS             3999..4823
                     /locus_tag="VFQ10_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET9966107.1"
                     /translation="MADQINYYEVLGVPREASQAEIRNAYRNLAKERHPDYPGGSAEE
                     FSRLQEANAVLSDPNRRRQHDEALDLAHAADQLAGLNLDFGSVEDELSAKRRERESGG
                     PSLGERLRGRFVRREEDPYESLRREARDSGRGRRTRGRYDVSEARWYEPHRFDPEPVT
                     LKSGAITFVAAFLAFIAIGQIGMWATGAVDPGPFGFFKVLAPFMPILYSLVGLVVAYF
                     AFKAAGYAALALSFVAALVVGGQGGPEGLLQFATVGIIVLLVVVYLGNRRESAARR"
     gene            4848..>6321
                     /locus_tag="VFQ10_11990"
     CDS             4848..>6321
                     /locus_tag="VFQ10_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HET9966108.1"
                     /translation="MAITTSDLERFISEFERRMAPLERAADEAWWNLATSGTNEAREA
                     FVHAGKEYNGLFSDRNEYMTLRGWFENLEVLESPLLQRQVEVLYRAFAERQGDEEILG
                     RIEELEAEANAIYGNHRSVVRDREMGENEVRELLRTSADQDLRRETWEASKSVGRAVE
                     GTVRELARLRNRLAREQGYENHYARSLELQEIEAPELARIMGLLESATDAPFRELKRD
                     IDTKLGLKFGVDAVMPWHLSGLFFQRATDIAGIDLDRYFEEEDLEDLTRRTFDAIGLD
                     VRGVISSSDLYEREGKNQHAFCARLGREYPYDVRVLANVRPDAYWMDAMLHEFGHAVY
                     DRHVNPRLPYLLRTYAHANTTEAIALMMGALVDDPGWLRSVAGANRERLDRDSDKLAA
                     HRRADRMILTRLVLVMYHFEQALYADPDSAELNSVWWDLVERLLFVHRPPGRNEPDWA
                     AVIHVAVAPVYYHNYMLGELISAQLRNYLESHITRGPFYQN"
BASE COUNT         1212 a         1919 c         2044 g         1146 t
ORIGIN      
        1 gggcatcgcg atttcggcgg ctggcgaagg attacgagag gctgcccgcc actttggcgg
       61 ggcttcactt tgtcgccttc gcttgcctct tcctccagca agcgagcggc atcctcggcg
      121 tgggttcata acacgctcta accgtagtca gggtcgctgc gggacagccg catgacgcag
      181 atgaacattt tgaaagccat ttcgtagtcc gaatcagtac ctgggcagcg gccctccggc
      241 gtcttccagg acgtagtcgt cgaacatacg ccgcgcgatg tcgggaagat ctcccgcgac
      301 gtcctcgtac atgccagggt cttctcgcag atcgtagagc ctggcgtccg tcccgtcgtt
      361 gcggctcatc atggcgtact cctcatccct ggtaaagacg tggtcgtggt atccgagcgt
      421 gaagtggggt ctgccctcgg gctctccacc ttccagcatc acggagaggt cctggccatc
      481 cagcgcttgc agaggttcga tcccgagaaa cccgagtatc gtcggcgcga cgtcgtgggt
      541 ggaggcgaag tagtcgctcg tcgcccccgc tcccttaccc tccggatggc ggatcaggaa
      601 cggaatgtcc gtaacctcag gccagagggt ggaggggagc ttaccgacga caccgtgttc
      661 cccgtgggcg ataccgtggt cggagaggaa tatgaggagg gagttgtcgg agaggtctag
      721 ctcttccatc ttgtccagaa agcggccgag ccagcggtcc gtcatcgtca cctctcccga
      781 gtagagggca ctcatccgcc ggatctgccg cccgctaagg tactcgctgc ctccgtccac
      841 gggcgcgaaa ggctcggggc cgtcgtaacc ctcgtcgtac atatctacgt actcttccgg
      901 agggtcccac ggtgcgtgag ggtcgtagca gtccaccata aggaagaacg gtctctcgga
      961 tctggtggtc tccaggaatt cggaggcccg ggtgaagact tgaggggcga accagtcttc
     1021 ttccgatcta cggtacgccg tattggcgaa gtatcgctgg aagtacgttc tgctccccgg
     1081 ccccacagcg gctttattca ccagcgcctg ctcgaccttc ccgggagggc aggtatagag
     1141 tggtctgtag ctgtctgtgg tctggccccg gataaaatcg aaggcgtcga agccacgctg
     1201 gaagttcatg gaggcgtcgt actgctgcag gttgtcggtg acgaacagcg tctcgaagtc
     1261 cgcgcttcgc atagtctccg cgagggtgat ctggtcgttt gggatcggct gccagccctg
     1321 aagaccgacg tctatgcccc tgtaacgtcg ccagtcctcg aaaggccagg tcctgaggcc
     1381 cgtgtgtatg gccctcctcg cgcagatcgt cggcgcggac tcggggtaag ccctactaaa
     1441 gcgcaggctt tcttctgcca gcgcatccag gttgggagtc tttatcttat cgttgccgta
     1501 gaccccgatg tgatccttcc tcaggctgtc catgatgata agcaccacgt tggtgtccct
     1561 gtcagcagac ggggtattgc ctgggaggtc agggagcaca cccacgcgtt cgcacccggc
     1621 gagcaacgat gccccggcta cccccgcgcc agacatcctc aagaactctc ttcgcgttaa
     1681 cgtccgcctc agcctctcct cctgcctacc gctcgcttgc acatccaatc atagagcaag
     1741 tcaagcggga agccccatcg tacccggaat cgccgcgggc gcagaatccg gccgagcgat
     1801 gtgatccagg tcaagccggt gtacaatccg ggcatgacgg tctttcgtta cgggcgggag
     1861 cacttcgagg aaggggagta cgtacgccgc gtggaagcga cgccgggtga cgacctcgac
     1921 cccgtagttc gcatcgtgga ggacctcggg accgtcgaga tcccctgcgg caaagtagat
     1981 tgccgcagta attgcctgga atacctggtc agcgccaccg gcaggcgaaa cgattacacc
     2041 ctagccgagt gcaagatctc cgcgctggta agcataatct gagcttgcca gccagtcagc
     2101 cttggcgtgc gcgagtcctt gctgacgtgc tgaaaagcta ctcctcccag gagtagcctt
     2161 tctgtgccgg tggtctttcg gcggcgtaga gccgctggag cacgcctcca gccagggggg
     2221 agaggaagag tcctgcgagg cctgccggca gcaggtcgcg ggcgatggtc caggctacga
     2281 ggagcggcac tcccggcgga tggttggaac cttctatgag gcgtagaggg tcgaggacgc
     2341 cgccgccagg gaggatgccc gtcgcgtaga cgatgagggt gagtacgccg gagataaacc
     2401 ccgtcagcag cgcaagcgcg aggctgagga cgaagcgggg acccgaacct tccgaacgac
     2461 cgcgccgcag gagcaaggcc ccgccgaaca aggggcctgc gataagccct gagagcaatc
     2521 cgacgcccat gtagacgagg gccagcgtgg tcagatcgcc ctcgctactc tgtccgccct
     2581 gcaggatcag gagcacgaaa ggctcgggaa acagcgattt gacgaacatg aagccaacgc
     2641 catagacggc gcctatcccc aaaagacgca gcaggatcac aatccaccca ccgcgccgcc
     2701 tgtgaggcat tgattgctcg tattcgtcga catgttctag aatttaacct ccaacggccg
     2761 aaacgtcagc aagagctttc acgaggcgcc gtctttgcgc gacgccgggt acaatcggcg
     2821 gcagcatgcg accagtacag gaccaggcag agatgcggcg cgttctcgag gagcgggcgc
     2881 tggaggccgg cttcgacctc gttggcgtgg cgcgcgccga gcctctgcgt gagggaggcg
     2941 agcgtctgcg ggagtggcag gaggcgggaa tggccgccga catgggctac atgcacagac
     3001 ccgtggagtt actctctgat ccgaggaagc tacagaagag cgcaaggagc gtggtctctc
     3061 tcggggtctc ttactatccc ggggaccatc ccgagaacga agtcgggggc agggtggcgc
     3121 gctacgcgtg gggccgagac taccacgagg ttataaaggg aaggctgttc cggctgaggg
     3181 aggatctcga ggaagagctt ggggtcagga taagagcccg cggtttcacg gacgccgtgc
     3241 cgctcctgga gcgctccgcg gcccagcatg cggggctagg ttttttcgga cggaactcct
     3301 gcctgataaa caacgagacc gggtcttact tctttatagc ggatctgatc gtggacctcg
     3361 acctagatcc cgacccgccc ggtaaaggga cctgcggacg ctgtacgcgc tgcatggaca
     3421 ggtgtcccac cggcgccata aaggctcctg gcgtcgtgga cgccaggctc tgtatctcgt
     3481 acctcacgat cgagaaccgg ggtgagatcc ctcgccagct ccgtccgaaa gtgggtgact
     3541 gggccttcgg ctgcgacgta tgccaggagg tctgtcccta caacaagacc aaggcttcga
     3601 ggagccgctg gcccgagttc tccgaagagg ccgggtcggg accttacctg gagatagaga
     3661 aggtcctcga gataagaacc gacgaggcgt tcgaggatcg ctttggagga acccctctca
     3721 cgcgccctag ccgggcaggc ctgttgcgca actgctgcgt ggccgccggg aacctgaaat
     3781 tggagaaggc tgtccctgcg ctggtacggg ccttgcgtgg ggacccttcg ccgctggtgc
     3841 gtgggcacgc ggcgtgggcg ctcggtgaga tcgggggtgc tgcagaatcg ctaatggagg
     3901 catccgaaac ggagaaggat acgtggtgtc tgcaggagat ccgactcgcg ctgacgaacg
     3961 ggaccacgga agctaccttg ggttagaata aggaaacgat ggcggaccag atcaactact
     4021 acgaggtgct gggcgtccca cgcgaggcct cccaggccga gatcaggaac gcttaccgca
     4081 acctggcgaa ggagcgtcac cccgattatc ccggcgggag cgcggaggaa ttctcccggc
     4141 tgcaggaggc caacgcggtc ctctccgacc cgaaccgccg ccggcagcac gacgaagcgc
     4201 tcgacctggc ccatgcggcc gaccagcttg ccggcctcaa cctggacttc ggctccgtgg
     4261 aagacgagct ctctgccaag cgcagggagc gcgagtcagg cggtccctcc ctcggcgagc
     4321 gcctgcgggg ccgcttcgtc agaagggaag aggaccctta cgagtccctg cggagagagg
     4381 cccgcgactc cggacgcggg cgcagaacca ggggccgcta cgacgttagt gaggcccgct
     4441 ggtacgagcc ccaccgcttc gacccggagc ctgtaaccct caagagtggc gccataactt
     4501 tcgtcgcggc cttcctcgcg tttatcgcca tcggccagat cgggatgtgg gcaacgggag
     4561 cagttgatcc cgggccattc ggtttcttta aggttctggc cccgttcatg cctattctgt
     4621 actcgctggt cgggctggtc gttgcttact tcgccttcaa ggccgcgggt tacgcggcgc
     4681 tggccctgtc cttcgtagcc gccctcgtcg tcgggggcca gggaggacct gaggggctct
     4741 tgcagttcgc gaccgtgggt atcatagtac tgctcgttgt agtttatctc ggcaaccgcc
     4801 gggagagcgc cgcccggcgc tgatcccgac cccttagaag gagcactttg gctattacca
     4861 cctcggatct ggagcgcttt atctccgagt tcgagcgacg catggctccc cttgagcggg
     4921 cggcggacga ggcctggtgg aacctggcga catctgggac gaacgaggct cgggaagcgt
     4981 tcgtgcacgc cggcaaagag tacaacgggc tcttctcgga tcggaacgag tacatgactc
     5041 tccgtggctg gttcgagaac ctggaagttc tggagagccc gctcctgcaa cgccaggtcg
     5101 aggtgctcta cagggcgttc gcggagcggc agggggacga agagatcctg ggccgcatcg
     5161 aggaactgga ggccgaggcc aacgccatct acggcaacca tcggagcgtt gtcagggaca
     5221 gggagatggg agagaacgag gtcagggagt tgctgcgaac cagcgcagac caggacctgc
     5281 ggcgggagac gtgggaggct tccaagtccg taggccgcgc ggtcgagggg accgtcaggg
     5341 agttggcccg cctaaggaac aggctagccc gcgagcaagg gtatgagaac cactacgcgc
     5401 gctcgctgga gcttcaggag atcgaagccc cagagctcgc caggataatg ggtttgctcg
     5461 agtcagcgac agacgcgccg ttcagggaac taaagaggga catcgataca aagctcgggc
     5521 tgaagttcgg ggtggacgcc gtcatgcctt ggcacctctc aggcctattc ttccaaaggg
     5581 ccaccgatat agcgggcatc gacctcgacc gctacttcga ggaggaggat ctggaggacc
     5641 tcacccgcag gaccttcgac gccattggat tggatgtgcg gggtgtgatc tcatctagcg
     5701 acctctacga gagggagggc aagaaccagc atgcgttctg cgcccgcttg ggacgtgaat
     5761 atccctacga cgtcagggtg ctggccaacg tcagacctga cgcctactgg atggacgcga
     5821 tgctccacga attcggacac gctgtctatg accgtcacgt caacccgagg ctcccctacc
     5881 tcctgcgcac ctacgcccac gccaacacga ccgaggctat cgccctcatg atgggcgccc
     5941 ttgtcgacga ccccggttgg ctccgttccg tcgccggcgc gaaccgagag aggctggacc
     6001 gagactcaga taaactcgcc gcccaccgcc gggcagacag gatgatcctc acccgtctcg
     6061 tgcttgtcat gtaccacttc gagcaggcac tctacgccga tcctgatagt gcggagttga
     6121 actctgtgtg gtgggacctc gtcgagcggc tgcttttcgt gcaccgtccg cctggcagga
     6181 acgagcccga ctgggctgcc gtcatccacg ttgccgtagc ccccgtctac tatcacaact
     6241 acatgctcgg ggagttgatc tcggcccagc taaggaacta cctggagtct cacataaccc
     6301 gaggcccctt ctaccagaac g
//
LOCUS       DASTKM010000299         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_121523, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000299 DASTKM010000000
VERSION     DASTKM010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121523"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1676
                     /locus_tag="VFQ10_11995"
     CDS             132..1676
                     /locus_tag="VFQ10_11995"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HET9966109.1"
                     /translation="MISTPSTPVDVPEPGSPEELGRFAALQAKLDPLARRVISDPRAS
                     QTVVVVPSLTLDVEELGKIAGAHHYEERMLCMLMLLRLPRTHVVYVTSQHIATAIVDY
                     YLHLLPGIPLRHARSRLTLLSCHDSSDVPLTQKILDRPRLLERIRSTIADTEAAHMTC
                     FNSTALERSLAVRLGIPLYGNDPQLNYLGTKSGSREAFREAGVHLPDGFEHLRDGDDV
                     VEAVVELKRRKPGLRRAAIKLDEGFSGEGNAVFPYEGAPEGRDLAGWVRNELPRRIKF
                     EAVGETWEHYGRKFEEMGGIVECWVEGREVRSPSVQCRINPLGEANVVSTHDQVLGGP
                     SGQIFLGSTFPADAEYSRDIKEAGDRVGEVLRERGALGRFAIDFISVRRGEGWEHAAI
                     EINLRKGGTTHPYLILRFLTDGTYDANDGLYCTPTAKPCYYYASDNLQSPAYKGLTPD
                     DLVDIAVDNGLHFDSASQQGVVFHLIGALSQYGKVGTVCIGDSHENALKYYRDTVAVL
                     DREAQP"
     gene            complement(1696..2112)
                     /locus_tag="VFQ10_12000"
     CDS             complement(1696..2112)
                     /locus_tag="VFQ10_12000"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HET9966110.1"
                     /translation="MLEQMQEHWREFKESKPGQRFKDRYRRRRQDEQGHILWRIFLIT
                     LGAVIALGSLVLAPLPGPGWGTVFIGLMILAGELLPAARFLDWLEVRLRTLGRYVYKI
                     WRASVWGKIAVILVAGLCVAAVAYVAYLLLSGVWLF"
     gene            2301..>2618
                     /gene="msrA"
                     /locus_tag="VFQ10_12005"
     CDS             2301..>2618
                     /gene="msrA"
                     /locus_tag="VFQ10_12005"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET9966111.1"
                     /translation="MTTDDRVNGPAREVATLGGGCFWCLEAVYQELRGVEKVESGYSG
                     GHVPNPTYRAVCSETTGHAEVVQVTFDPSVVSYKDVLEVYFTIHDPTTLNRQGADVGT
                     QYRS"
BASE COUNT          520 a          781 c          852 g          465 t
ORIGIN      
        1 tcgtgaaacg aactggggtt cattaccgtt tcctggtgag cagatagagg accggggatg
       61 ttgacgaggg ctcttctgcg ggacgtggga gcctcgcgta taattttcgc ccagggagca
      121 ggagtgacag catgatctcc acaccatcga cgccggttga cgtgcctgag ccgggctctc
      181 cggaggagct cgggcgcttc gcggctttgc aggcgaagct cgacccgctg gcgcggcggg
      241 tgatctctga tccgcgcgcc tcccagacgg tcgtcgtggt gccgagcctc acgctcgacg
      301 tcgaggagct ggggaagatt gccggtgcgc accactacga ggagcggatg ctttgcatgc
      361 tgatgctctt gcgcttgccc cgcacccacg tcgtctacgt cacgagccag cacatcgcga
      421 cagccattgt ggactattat ctgcatcttc tgcctggcat cccattgcgc cacgcccgca
      481 gccggctgac gcttctctcc tgccacgact cctccgatgt gccgctgacg cagaagatac
      541 tcgaccgtcc gcgtctgttg gagcggatac gctctacgat agcagacacg gaggcggcgc
      601 acatgacctg cttcaacagc acggcgctgg aacggagtct cgccgtccgg cttggcatcc
      661 cgctctacgg caatgacccg caactcaact acctcgggac caagagtggc agccgcgagg
      721 ctttcaggga ggccggggtc catctgccag atgggttcga gcacctgcgt gacggggacg
      781 acgtagtcga ggccgtcgtc gaactcaagc gccgcaaacc cgggctgcga agggcggcca
      841 tcaagctgga tgaaggtttc tcgggtgagg gaaatgcggt gttcccttat gaaggggcgc
      901 ctgagggtag ggatcttgcg ggttgggtca ggaacgagtt gccgaggcgc atcaagttcg
      961 aggcagtggg agagacctgg gagcattacg ggcggaagtt cgaggagatg ggcggcatag
     1021 tagagtgctg ggtggagggg agggaggtgc gctcaccttc cgtgcagtgc cgaatcaacc
     1081 ctctaggcga ggcgaatgta gtctccaccc acgaccaggt cctgggaggg ccttcaggcc
     1141 agatcttcct cggcagcacg ttccccgctg acgccgagta cagccgcgac atcaaggagg
     1201 ccggagacag ggtgggggag gtgctcaggg aacgaggcgc cctgggaagg ttcgccatcg
     1261 actttatctc cgtcagacgc ggcgagggct gggagcacgc ggctatcgag atcaaccttc
     1321 gcaagggcgg caccacacat ccgtacctga tcctgcgttt cctgacggac gggacgtacg
     1381 acgcaaatga cggactgtac tgcacgccga cggcgaagcc ctgctactac tacgcttctg
     1441 ataacctcca gagccccgca tacaagggcc tgacccctga cgatctggtg gatatagctg
     1501 ttgacaacgg cctccacttc gacagcgcct cccagcaggg ggtggttttc cacctcatcg
     1561 gggcgctctc ccagtacggc aaggtcggca ccgtctgcat cggcgacagc cacgaaaacg
     1621 ccctgaagta ctatcgggat accgtcgcgg ttctagaccg tgaggcgcag ccctaacggg
     1681 atcggccgta cctgctcaga acaaccagac gccggaaagc aacaggtagg cgacgtaagc
     1741 gaccgctgcg acacagagcc cagccaccag gatcaccgcg atcttacccc aaacagaagc
     1801 ccgccagatc ttgtagacgt atcgtccgag cgtcctcagc cgtacctcga gccagtcgag
     1861 gaagcgggcc gcggggagga gctcgccggc caggatcatg agcccgatga agactgtgcc
     1921 ccagccgggg ccaggcagcg gggcgagtac caggctgccg agagcgataa cggcgccgag
     1981 ggtgatcagg aagatcctcc ataagatgtg cccctgttcg tcctgccgcc ggcgacggta
     2041 acggtccttg aagcgttgcc ctggcttgct ctctttaaac tcccgccagt gctcctgcat
     2101 ctgctcgagc atacatagat aattcctcat cgagacccat ttgacaacct cgcgacgagg
     2161 tttccgcctt cgtcgaggag gggtgaccat gcccgacaag aatggtaggt tcaacggaga
     2221 accgcccaga gccctgcacc cgagtaacgg atgcggtata ccgagggtgt cgaaaagata
     2281 cgaacggaga gatgtgagcc atgacgactg acgatagggt gaacggccct gcgagggagg
     2341 tcgccacgct cgggggcggc tgtttctggt gtctcgaggc ggtctaccag gagctgcggg
     2401 gcgtggagaa ggtcgagtcc gggtactctg gcggccacgt gcctaacccg acttacaggg
     2461 cggtgtgctc ggagacgacg gggcacgccg aggtcgtgca ggttacgttc gatccctcgg
     2521 ttgtctctta caaagatgtt ctcgaggtct acttcaccat ccatgatccg acgactttga
     2581 accgtcaggg cgcggacgtg gggacgcagt accggtct
//
LOCUS       DASTKM010000300         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_473460, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000300 DASTKM010000000
VERSION     DASTKM010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473460"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFQ10_12010"
     CDS             complement(<1..566)
                     /locus_tag="VFQ10_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HET9966112.1"
                     /translation="MTLASQYVLGMRVDATSYEDASQRVARWAHDGRSAYVCVATVHM
                     TMEAFDSSAFQRVVNGADLITPDGRPLVWALRSLGVRDATQVRGTDLTTHVVERAARE
                     GIPIGLYGGTPDLLKTFVRILERRYPGVRVVCQIAPPFRPLTPEEDETVTKEIRSSGA
                     RILFVGIGCPKQERWMETHKDRIPAVMLG"
     gene            complement(563..1741)
                     /locus_tag="VFQ10_12015"
     CDS             complement(563..1741)
                     /locus_tag="VFQ10_12015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9966113.1"
                     /translation="MKILAVHNRYQRPGGEDQVFVDETALLETRNHRVLRYEVHNDQV
                     KQVNRLTLAKDTIWNSSAYRELGAMIRRERPDVVHFHNTLPLVSPAGYYVARAEGVPV
                     IQTLHNYRLLCPVALFFRDGRVCEDCMGKAVPWPGVVHRCYRGSRAASGVIATMLTVH
                     RALRTWTEMVDVYVALTEFARDKFIEGGLPAGKIVVKPNFVAPDPGQGPGGGGYALFV
                     GRLAPEKGTGTMLAAWNRLGTRIPLKIVGDGPLRDRVMGAAARQSNVEWLGHRPVADV
                     HELMGKADMLVFPSQWYETFGRVAAEAFAAGTPVIAANIGAVAELVEHGRTGLKFRPG
                     DSGDLVTQVEWALSHPAELRSMREEVRAEFEAKYTAERNYRTLMEIYEAALERKKVPA
                     "
     gene            complement(1769..2932)
                     /locus_tag="VFQ10_12020"
     CDS             complement(1769..2932)
                     /locus_tag="VFQ10_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9966114.1"
                     /translation="MRPYVQGTDASALLGVRLETDKTAGRMYAAYPNLANVRWLLPTK
                     QPALRRAGIEGLYQPSSLRGRALRTIIGAGALQGQKVWLEEDNLQRLETMLASVMGVE
                     AVRLAFYLGVPAPHRKVTAQVLTPEGKTLAYAKIATSQLAQAALKTERRALSRLSQSA
                     GLQGTVPEVLGSLEWHGGTILLITMGPPRPGPRGLSSAHLRFCTQLFQSFRGHQTFVE
                     SPMWSSMSEIWLHLQRSSPETLPANLGPALERLHDELGPVNMPLSLAHGDFAPWNTRL
                     GERSLFVLDWERASEGMTPLYDAFNFQALQSALLGRRKGIRDRLFLLRLLDAIWPEGH
                     KHLPILYLAYLTHVTLLYSEAQSLVPGVGDKKVWHWFAQQIESALHEDASSMP"
     gene            complement(3265..>4207)
                     /locus_tag="VFQ10_12025"
     CDS             complement(3265..>4207)
                     /locus_tag="VFQ10_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9966115.1"
                     /translation="QAHNIALRERAVKMGINISEYGLAKAGTGDYKPVATEEDLYSRL
                     GLAYIPPELREDTGEIEAADRGRLPDLVSVEDVRGDLHVHTNYSDGKGTIVSMAEAAI
                     ELGYEYLVFCDHSQSLRVANGLSPERLKKKMEAVRKADEGYGEIHLLCGSEVDILRDG
                     SLDYDDDVLAGLDFVVASVHTSFGVGEKAMTERIVRAMNNPYVRTIGHPTGRILNRRE
                     PYEVDVSRVIREAAATNTALELNAYVDRLDLAVPYVREAVGAGVSITIDTDAHDERAL
                     AFMKYGVSQARRAWVEKGSVINCLPLDEFEKYLKNGK"
BASE COUNT          795 a         1333 c         1215 g          864 t
ORIGIN      
        1 cccagcatca ccgctggaat gcgatcctta tgtgtctcca tccatctctc ctgcttggga
       61 catccgatac ctacgaacag gatacgtgca cccgaggacc gtatctcctt cgttactgtt
      121 tcgtcttcct ctggtgtcag cggacgaaag ggtggtgcga tctggcacac cacccgcacc
      181 ccggggtagc gtctttccag gatacgaacg aacgtcttta gcaggtctgg cgtgccaccg
      241 tacagcccga tcggtatgcc ttcccgcgcg gctcgctcca ccacgtgcgt cgtcaggtcc
      301 gtcccccgga cctgggtcgc gtctctaacc cccaagctcc tcaaggccca aaccagaggt
      361 cttccgtcag gcgtaatcag gtctgccccg tttaccaccc gctgaaacgc cgaggagtca
      421 aaggcctcca tggtcatgtg cacggtcgcc acgcacacgt aagctgagcg cccatcgtgt
      481 gcccatcgtg ctacgcgctg ggaagcgtcc tcgtaggagg tagcgtctac ccgcataccg
      541 agcacgtact gcgaagctag ggtcaagccg gcaccttctt gcgctccagg gcagcctcgt
      601 agatctccat cagcgtccgg taattgcgct ccgcggtgta ctttgcctcg aactcggccc
      661 tgacctcctc acgcatactt cgtagttccg ccgggtgcga cagggcccac tcgacttgtg
      721 tgacgagatc tcctgagtcg ccgggacgga atttgagtcc cgtgcggccg tgttctacga
      781 gttcagcgac cgctccgatg ttagctgcga taaccggagt gcccgctgcg aaagcttctg
      841 ccgctacccg gccgaacgtc tcgtaccact gtgaggggaa gacgagcatg tcggctttcc
      901 ccataagctc gtggacatct gccaccggcc tgtgtcccag ccactccacg ttggactgtc
      961 tagcggcagc tcccattacc cgatccctca atggtccgtc tcccacgatc ttgagcggga
     1021 tcctcgttcc gagccggttc caggcggcta gcatcgtccc tgtccctttc tcgggcgcga
     1081 gccgccccac gaacaaggcg tacccgcctc ctcctggccc ctgaccagga tcgggggcaa
     1141 cgaagttggg tttcaccacg atcttccccg cagggaggcc accctcgatg aatttgtccc
     1201 gggcaaactc tgtaagggcg acgtagacat caaccatctc tgtccatgta cgcaaagcgc
     1261 ggtggacggt gagcatcgtc gctataaccc cgctcgccgc ccggctccct cggtagcacc
     1321 tgtgaacgac gccaggccag gggaccgcct tgcccatgca atcctcgcac acccgcccat
     1381 ctcggaagaa gagagctacg ggacagagca gacggtagtt gtgcagggtc tggatcactg
     1441 gaaccccttc ggccctggcg acatagtaac cggccgggga gactaggggt agagtgttat
     1501 gaaagtgaac cacgtcgggc cgctcccggc gaatcatggc cccgagttca cgatacgctg
     1561 aagaattcca tattgtgtct ttggccaggg tcaaacggtt cacttgcttg acctgatcgt
     1621 tatgcacctc gtaacgcaac acgcggtgat ttcgggtctc caagagagcg gtttcgtcca
     1681 cgaaaacctg gtcctcgccg ccggggcgct ggtacctgtt gtggaccgcc aagatcttca
     1741 tctggcacaa gttaccatac ccgcattttc agggcatcga tgacgcatcc tcgtgcaggg
     1801 cgctttctat ctgctgcgcg aaccaatgcc agaccttctt gtctccgacg ccggggacta
     1861 ggctctgggc ctcgctatag agcagggtga cgtgtgtcag gtaagcaaga tacaaaatgg
     1921 gcagatgttt gtgtccttcc ggccagatgg cgtccaacaa cctcagaaga aacaggcgat
     1981 cccgaatacc cttacgccga cccagcaggg cggactgcag ggcttggaag ttgaacgcgt
     2041 cgtacagggg agtcattcct tcggaggcgc gctcccaatc gaggacgaac agggatcgct
     2101 ctcccaatcg ggtattccag ggtgcgaagt cgccgtgagc cagagacaga ggcatattca
     2161 ccggccccaa ctcgtcgtgc agcctctcaa gggctgggcc gagattagcg ggcagggttt
     2221 cgggtgagct gcgctgaaga tggagccata tttcggacat gctactccac atcggactct
     2281 ccacaaacgt ttggtgcccc ctaaaggact gaaaaagctg cgtgcagaaa cgcaggtgcg
     2341 ccgatgacag tcctctagga cctggacgcg ggggccccat cgtaatgagg agtattgtgc
     2401 ccccgtgcca ctccagagaa cccaatacct ctggtactgt gccttgcagg ccagcgctct
     2461 gcgataaccg cgacagggca cgcctttcgg ttttgagtgc cgcctgggcc aactgagaag
     2521 tggcgatctt ggcgtaagcc aacgtcttcc cctccggggt gagtacctgc gcggtgacct
     2581 tccggtgcgg ggctggtacg cccagataga aagccaagcg taccgcctcc actcccatga
     2641 cggatgccag catcgtctcc aagcgttgaa ggttgtcctc ttccagccat accttttgac
     2701 cctgaagagc tccggcgccg atgatggttc gcagtgcccg gccccgcaga ctactcggtt
     2761 gatagagccc ttctataccg gcccggcgca aagcgggctg tttggttggg agcagccagc
     2821 ggacattggc aaggttggga taggctgcgt acatgcgccc cgccgtcttg tcggtttcaa
     2881 ggcgaacccc caggagcgcg gaagcatcgg ttccctgcac gtaagggcgc atacctaacc
     2941 ttcttcttgt cggatcagat ccgccacgca accgctacac gagaaaggag gccaaccatc
     3001 ctatcctact tccggctgca acaatctgca atccttgagg gagcttctca ttacatgtat
     3061 ttaacctaac cttagcaggt tacgcccgag aattgtcgtt tgttgcattc ccttcttgga
     3121 aacccgtcct ttgaggtcac ccggtcggat ggacgagcgg aagatgctcg gcgagcccac
     3181 ggtcaacgag ctagtggcgc actatctcgc ccggtgaatt gtagcgttcg gcaaggtcag
     3241 agaaggggtc ttgcaccggc atgactactt cccgttcttc aggtacttct cgaactcgtc
     3301 caaggggagg cagttgatca cactcccctt ctcgacccag gcccttctcg cctgcgagac
     3361 gccgtacttc ataaaggcaa gtgcccgctc gtcgtgggca tctgtgtcga tggtgatgct
     3421 gacgcctgct cccacggcct cgcggacgta gggaaccgcg agatcgagac ggtccacgta
     3481 ggcgttgagc tcgagggccg tgttcgtggc ggcagcctcc ctgatcacac gcgacacgtc
     3541 cacctcataa ggttccctgc ggttcaggat acggcctgta gggtggccga tagtccgcac
     3601 gtacgggttg ttcatcgccc gcacgatgcg ctcggtcatg gccttctcgc cgaccccgaa
     3661 agaggtgtgc acggaagcaa cgacgaagtc cagcccggcg agcacatcgt cgtcgtagtc
     3721 gagggagccg tccctgagga tgtcgacctc ggagccgcag agaaggtgga tctcaccgta
     3781 gccctcgtcg gcctttctca ccgcctccat cttcttcttc aggcgctcgg gcgagaggcc
     3841 gttcgccacc ctgaggctct gggagtggtc gcaaaagacg aggtactcgt agccgagctc
     3901 gatggccgcc tcggccatgc tcacgatggt gcctttgccg tcagagtagt tggtgtgaac
     3961 atgcaggtcc ccgcggacgt cttcgacgga gacgaggtcg ggcaatctgc ctctatctgc
     4021 ggcttcgatc tcaccagtgt cctccctcag ctccggcggt atatatgcga ggccgagccg
     4081 cgagtagaga tcctcctccg tggccacggg tttgtagtcg ccggtcccgg ccttggccag
     4141 gccgtactcg gagatgttga tacccatctt gacggcccgc tcgcgcagcg cgatgttgtg
     4201 ggcctgg
//
LOCUS       DASTKM010000301         6402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_301959, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000301 DASTKM010000000
VERSION     DASTKM010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301959"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..447)
                     /locus_tag="VFQ10_12030"
     tRNA            complement(360..447)
                     /locus_tag="VFQ10_12030"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            964..1200
                     /locus_tag="VFQ10_12035"
     CDS             964..1200
                     /locus_tag="VFQ10_12035"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HET9966116.1"
                     /translation="MREGHYGDVRLDGLSLIGMGGFEGNIWAGAEGPFGLFIDERADE
                     RQHEALQMIFGGQAGGWPADFAEGVGEMHGIKVR"
     gene            1353..2828
                     /locus_tag="VFQ10_12040"
     CDS             1353..2828
                     /locus_tag="VFQ10_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9966117.1"
                     /translation="MGKKGERKAMRPSNGLTRREFLRITGAGLAGASFLGISGCGEEG
                     AGGGAAGGDVWKQFKGTTLQFISENTPPTAAIAANLKPFKELTGIDVKITQIELGALV
                     EKVALDFGSGEGQYQIIYADPYQVLAPYYQGLAELNAFNEDDSLPSIPKGTGDFIPTQ
                     LDAAGKFESEEELYALAYDAPTMIWMYRKDLFEKYGSQMEQDLGFDPKPSDSSTWDQY
                     YQIAEWFNDNANEVKYGTGHQAKQYDSLMCDFSNILWAYGGDYFENGVEVGRLGSEDP
                     GPAQLDQPKALEAAEFYNKLLSIAHPGSTTWDWSGVDEAFRAGELAMTPNWHEFAAGI
                     ETSDLGGKVGYTRLPTGPARAADMYGGTGIGINSGAPEDEQRAAWLFLVWATSPQTQR
                     MGLFSEEGGGTPTRQSVYDMPEVQKAKTRPSDAPNMLTADAVFKAWESQNIGLRPKIQ
                     QWNECDTIIFTELSKMLAADKSPEQAMQDAKESFDRATGAA"
     gene            2834..3748
                     /locus_tag="VFQ10_12045"
     CDS             2834..3748
                     /locus_tag="VFQ10_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9966118.1"
                     /translation="MSVARTDKDRGLNRLLRMPSFGVRMMLPALLLLAALSIFPFLFI
                     VWMSFNEVSPIGGLSFEWAGLENWVNLFADPAVRSSWVLSAIYFVATVGLEMFLGIAV
                     ALLIHELVWGKNLALSLFLMPIFIAPVMVGLLGRFLVDPTYGLYAWVLNATGIFTKNI
                     LGSGESALVAVILMDVWEWTPLVTIIVLAGLSSMPGEVIEAARVDGASYWQRLRYIVM
                     PLISGVAIVALLVRSMDAIRFFDIILVTTNGGPADATKIIPIRLYETAFRFFDLGYAA
                     AIGLGMLAFSIVIANLFLRILKAQGLAR"
     gene            3745..4584
                     /locus_tag="VFQ10_12050"
     CDS             3745..4584
                     /locus_tag="VFQ10_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9966119.1"
                     /translation="MTESSRRWSSQIVLYALLALILLWTLVPIVWMVLSSFKSPDDIL
                     STTPKVIFEPTLQHYQGLFTGSNSLGGYVKNSLLAAGISTIISVALACLAGYGLARSH
                     FRGKQHLSFWIISTRMAPIAAVIVPLYLIFRNLDLLDSVIGLIIAYLTFNLPFAIWLM
                     NAFFADLPVSLEEAAMVDGATRFQAFWRVALPLVTPGIVTTAVLCLIFAWNDYAFART
                     FSGPESQTIPIAASQLITQTGIDWGKLTAIGTVVVLPMVIVGLAVRRWLVRGLTLGAV
                     KGE"
     gene            4613..5641
                     /locus_tag="VFQ10_12055"
     CDS             4613..5641
                     /locus_tag="VFQ10_12055"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9966120.1"
                     /translation="MQNLAAIMYGTHDIRLEDKPVPEPGSKEVLVEIRAVGVCGSDVH
                     YYEEGRIGSFVVREPLILGHESMGTVVALGEEATKHEVGERVAVEPGVPDMTCRECRA
                     GHYNLCPNVRFFATPPIDGAFTNYVTIHEDFAFALPDNLSDNAGALMEPLSVGIWACR
                     KANVRAGNHVLVTGAGPIGLLAMQVALAEGATEVTITDVSQERLEIAEKTGATRAINV
                     SEEQLAEADVEVDALIECSGNEVALGDGIRCIRPAGTAVVVGMGPGEESSIPLALIQN
                     KEIWLTGIFRYANTYPAAIELAATGRVDLDAIVTSYYGLEDTEEALQASRKDPANVKP
                     MVIANSSE"
     gene            6207..>6402
                     /locus_tag="VFQ10_12060"
     CDS             6207..>6402
                     /locus_tag="VFQ10_12060"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9966121.1"
                     /translation="MSISVVWPGREGIEVEPRDVPPPGLGELKIRIEASGLCGTDLHI
                     ASGEYPFAQPGVTIGHEFAGT"
BASE COUNT         1275 a         1806 c         2049 g         1272 t
ORIGIN      
        1 gagggtcctt caaaaggagt cggtactgcc aatcgcgttc cttggcgttg gcagccccag
       61 agttggcggg ggccgcagcg gaagcgaagc tggcggcctt tagggcgtag tgggcagcgt
      121 gcgggcgtgc gccccaacgt gggcggcgga tgcggcgacc tcgtaccccc accgttcata
      181 ctcaaatgtt ctcgcagcct tcctgtccta gatcggccac gtctatgttt ctataaagga
      241 cgactcttca gagctgtctt cagcgcgttg ctgcacggtt gctgcacaaa agctgggccg
      301 tggttggggt cactatcttt ggggctacga aagtcctgca aagtagtgca tttaacaagt
      361 gggcgatgct gggatcgaac cagcgacctc tgccgtgtga aggcagatcc aggatgtcgg
      421 agttgttcgc cgttgttcac aatcgcctgc aaaacagcca attcgcctct acgatgtttc
      481 tcggttgttc gccgccgttc gtgtgagttg gtgtactact ggtgtaaaca agactctgcg
      541 caacaccagc actttcgcat ttctccatga tctccggcct atccagtgcg cctgccagga
      601 ttcggatctg ttactggcgg attacgggcg tttcgaagat attgacccat gcaaagtatg
      661 aattcatgaa ctcttgcgca gctcttcaga tcagacgtcc tgtacggctc cgagttcggt
      721 ctgcgcaatc tctgaagggt gcccttaccc aaaaggatgc attgggcgtc acaattcggc
      781 ataatgctag tgcgggatgc ggggaaagga gaagggaaca tggaacccgg tgctgcaagt
      841 gacgtccctc agtggcatgt ggtggggact ggttcgaggt ctgcaggtgc gacatcccct
      901 tcccctctga gttcgcgcgg cttcctagcc gaaaattact gtgagggcgt gctcgcttag
      961 cacatccgcg agggacacta cggcgacgtt cggctggacg gcctgagcct tatcgggatg
     1021 ggcggcttcg agggcaacat ctgggccgga gcagagggcc cctttgggtt gttcatcgac
     1081 gaacgagccg atgagcggca gcacgaggcc ttgcagatga tcttcggtgg gcaagcaggc
     1141 ggctggccgg cagacttcgc cgagggtgtc ggcgaaatgc acggcatcaa ggtcaggtag
     1201 tagactagtg aaaatcggta cgaggtgagc tgtgtttggg gtgagttggt ttcagggtaa
     1261 aggccaatgg acggtggtcg acacggggaa tgggccgggc cgttgggagg gccagcttga
     1321 cgggaaattc ggcgcggaga agaattgcgt ctatggggaa gaaaggtgag agaaaggcga
     1381 tgagaccttc gaacgggctg accaggcggg agtttctcag gatcacagga gctgggttag
     1441 ccggagcgag cttcctaggg atatccggct gcggtgagga gggggcgggt ggtggagccg
     1501 ccggcggaga cgtgtggaag cagttcaagg gcacgacgct ccagttcata tccgagaaca
     1561 ccccgccgac ggcagcgatc gcggcgaacc tcaagccgtt caaggagctt acgggcatcg
     1621 acgtgaagat cacgcagatc gagctgggtg cactggtgga gaaagtggcg ctcgacttcg
     1681 gttccggaga aggtcagtac cagataatct acgccgatcc ctaccaggtt ctggctccgt
     1741 actaccaggg gcttgcggag ctgaatgcgt tcaacgaaga cgacagtctc ccctcgatac
     1801 ccaaaggtac gggggatttc atacccactc agctggacgc ggcgggcaag tttgagagcg
     1861 aggaggaact gtacgccctg gcctacgacg ctccgacgat gatctggatg tatcgcaagg
     1921 acctgttcga gaagtacggc tcgcagatgg aacaggacct cggcttcgat ccgaagccct
     1981 cagattcatc tacgtgggac cagtactacc agatcgccga gtggttcaac gataatgcaa
     2041 acgaagtgaa atacggcacg ggccaccaag ccaagcagta cgactcgctg atgtgcgact
     2101 tctcgaatat cctctgggca tacggcggcg actattttga gaacggcgtc gaggtgggga
     2161 ggctaggcag cgaggatccc ggccctgccc aactcgatca accgaaggcg ctcgaggcgg
     2221 cggagttcta caacaagctc ctaagcatcg cgcacccggg gagcacgacc tgggactgga
     2281 gcggcgtgga cgaggccttc agggccggcg agctggcaat gacgcccaac tggcacgagt
     2341 tcgcggccgg gatcgagacg tcagacctgg gcggcaaggt cgggtacacg aggctaccca
     2401 ccggacccgc aagggctgcc gacatgtacg gcgggaccgg catcggcatc aatagcggtg
     2461 ctcctgagga cgagcagcgg gcggcctggc tcttcctggt gtgggcaacc tcgccccaga
     2521 cccagcgcat gggcttgttc agcgaggagg gaggcggcac acccacgcgg cagtccgtct
     2581 acgacatgcc ggaggttcag aaggccaaga cgcgaccgtc ggatgcgccg aacatgctca
     2641 ccgcagatgc ggtgttcaag gcgtgggaat cccagaacat cggccttcgc cccaagatcc
     2701 aacagtggaa cgagtgtgac accatcatct tcaccgaact ctccaagatg ctggccgccg
     2761 acaaatcgcc cgaacaggct atgcaggacg ccaaagagag cttcgaccgg gctaccggag
     2821 cggcttaagc gccatgagcg tcgctcggac cgacaaggac cgggggctaa atcggctctt
     2881 acggatgccg tcgtttggcg tcaggatgat gctgcctgct ctgctgctcc ttgccgctct
     2941 ctcgatcttt ccgttcctgt tcatcgtctg gatgagcttc aacgaggtgt cgccgatcgg
     3001 gggcctctcg tttgagtggg cggggctcga gaattgggta aacctctttg ccgacccggc
     3061 cgtacgctcg agttgggtgc tcagtgcgat ctatttcgtc gccaccgtcg ggctcgagat
     3121 gtttctcggc atcgccgtcg ctctgctcat tcacgagctc gtctggggca aaaacctagc
     3181 gctttcactg ttcctgatgc cgatattcat agccccagtg atggtgggtc tgctgggccg
     3241 gtttctggtt gatccgactt acggactata cgcttgggta ctcaacgcaa caggtatttt
     3301 cacgaagaac atcctcggaa gcggagaatc ggcactcgta gccgtcatct tgatggatgt
     3361 ctgggagtgg acgccgctgg tcacgatcat cgttctcgcc ggcctctctt cgatgccggg
     3421 tgaggtgatc gaggcggcgc gggtggatgg tgccagctat tggcagaggc tgcgttacat
     3481 cgtgatgccg ttaatctccg gtgtcgccat cgtggcgctt ctggtccgtt cgatggatgc
     3541 tattcgcttc tttgacataa tcttagtcac taccaacggt ggcccggccg acgcgaccaa
     3601 gatcatcccg atccgactct atgaaacggc tttccgcttc tttgacctcg gctacgcggc
     3661 ggcgatcggc ctcgggatgc tcgcgttctc gatcgtgatc gcgaacctct tcttgcgaat
     3721 actcaaggct caggggcttg caagatgacc gagagtagcc gcaggtggag tagccaaata
     3781 gtgctctacg cgctcttggc gctgatccta ctctggacgc tcgtgccgat cgtctggatg
     3841 gtgctctcgt cgttcaaatc tcccgacgat atcctctcga ctacgccgaa ggttatcttc
     3901 gagcccaccc tacaacacta ccagggtctc tttacgggga gcaacagcct gggcggttac
     3961 gtcaagaaca gcctgttggc agccgggatc tcgacgataa tctccgtcgc tctcgcctgt
     4021 cttgcgggat acgggctcgc gcgctcccac ttccgcggca agcagcacct ctccttctgg
     4081 atcatcagca cccggatggc accgatcgcg gccgtgatag tgcccctgta cctgatcttt
     4141 cgtaaccttg acctgctcga ttccgtgata gggctcatca tcgcctacct tactttcaac
     4201 cttcccttcg cgatctggct catgaacgcc tttttcgcgg atctgcccgt gtcgcttgag
     4261 gaggcggcga tggttgacgg tgcaacgagg tttcaagcct tctggcgcgt cgcactgccg
     4321 ctagtaacgc cgggcatcgt gaccaccgcc gttctctgcc tcatattcgc ttggaacgac
     4381 tacgcctttg cgcggacctt cagtggtccc gagagccaga caatccccat agctgcctcg
     4441 cagctgatca cccagaccgg gatcgattgg ggcaagttga cggcgatcgg cacggtggtg
     4501 gtactcccta tggtgatcgt cggccttgcc gtccgtcgct ggctcgtgag aggcctaacg
     4561 cttggagcgg tcaagggaga ataagaagca cgaaacaaag aaaggagccg caatgcagaa
     4621 cctggctgcc atcatgtacg gcacgcatga catccggctc gaagataaac cggtccccga
     4681 gccgggctca aaggaggtgc tcgtagagat aagggccgtc ggcgtttgcg gctccgacgt
     4741 gcactactac gaggaaggac gcatcggctc gttcgtcgtg cgcgagcccc tgatcctggg
     4801 ccacgagtcg atgggaaccg tcgtggcttt gggagaagag gccaccaaac acgaggtcgg
     4861 tgagcgggtg gcagtggaac cgggcgttcc cgatatgacc tgccgcgagt gccgggcagg
     4921 ccactataac ctctgcccaa acgtccgctt cttcgcgacg ccccccatcg acggcgcctt
     4981 cacaaactac gtgacgatcc atgaggactt cgcgtttgcg ctgccggaca acctctcgga
     5041 caacgctggt gcgctgatgg agccgctctc ggtaggcatc tgggcctgcc ggaaggcgaa
     5101 cgtccgggcc ggcaaccacg tgctggtgac gggcgcggga ccgatcgggc tcctcgcgat
     5161 gcaggtggcg ctcgccgagg gcgcgacgga ggtcaccatc accgatgtct cccaagagag
     5221 gctggagata gccgagaaga ccggcgccac tagggccata aacgtctccg aggaacagct
     5281 tgcggaggcc gacgtggagg tggatgccct tatcgaatgc tcgggcaacg aagtcgcgct
     5341 gggcgacggc atccgctgca tccggcccgc cggcaccgcc gtcgtggtcg ggatgggtcc
     5401 cggcgaggag tcgagcattc cgctggcgtt gatccagaac aaggagatct ggctgaccgg
     5461 tatcttccgc tacgcgaaca catacccggc cgcgatagag ctggccgcca cagggcgcgt
     5521 ggacttggac gcgatcgtga cgagctacta cggcctggag gacacggaag aagcgttaca
     5581 agcgagcaga aaagaccccg ccaacgtcaa gccgatggtc atcgcgaact cgtcggagtg
     5641 aggccgtccc ggctcaggag caaggaggag cactacgagg tctaacgctc gttgtacggg
     5701 gcggcacggg cgatgcaccg cctaacggag cccataaccc gcgatggtat ggagctctta
     5761 tcttccttaa tgcctgctgg tctaaagagc agaatttggc cggtacacgg aggaagaagt
     5821 gggccgaaaa gtaccctatc gacggttcgt agggctacgc cgcagggacc tctgagactt
     5881 ttcggacgat ttctggacgt aatccgattc tcggaagtgc tcgcgttcta aggacgtgta
     5941 gctttgagag atgtagctat cgtgttgcgt cttggaaacg ttcgcgggcc caggtagtca
     6001 gacctcgctg gcggtcgcct cccagctgat aacgcagacc gggataaacc ggggccagct
     6061 gacggcgata gggacagtgg tggcgctgcc gatagcactc gcgggcctcg ctatcaggcg
     6121 ctacttggta cgcgctgact ctaggcgcgg ttgccggggg tgaaggagag agtgtgaaat
     6181 tcgaagacac agggggtggt taaatcatga gcataagtgt ggtctggccc gggcgggaag
     6241 ggatagaagt tgagccccgt gacgtaccgc caccgggtct tggcgagtta aagatccgga
     6301 tcgaggcttc gggattgtgc gggaccgacc ttcacatcgc ctccggtgag tacccgtttg
     6361 cccagccggg cgtaaccatt gggcacgagt tcgcggggac ca
//
LOCUS       DASTKM010000302         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_653447, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000302 DASTKM010000000
VERSION     DASTKM010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653447"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1243
                     /gene="ruvB"
                     /locus_tag="VFQ10_12065"
     CDS             158..1243
                     /gene="ruvB"
                     /locus_tag="VFQ10_12065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET9966122.1"
                     /translation="MNTEEMDDFTVRGEGRTEELPEDITGAAGFGEDPLDPEAYAEDD
                     EPSLRPSSFDEFVGQEALKQNLKIFVQAARQRDEALDHILLDGPPGLGKTTLCRILAE
                     EMGVGLRPTSGPTLERAGDMAAILTSLEERDFLFIDEIHRLNRQIEEVLYPAMEDFAM
                     DIVLGQGPSARTIRMDLPRFTLVGATTRKGLLTKPLLDRFGVAERLEYYGPRDLEKIV
                     VRNAKILGVPITEGGARQLARRSRGTPRIVNRLLKRVRDYAQVVGDGKIDEETANAAL
                     DMQGVDHLGLDRADRVYLGLVIDKFEGGPVGIGTISVALGEARDTVEDVYEPYLLQSG
                     LIQRTPRGRVATQRAYGHLGVPVPNNG"
     gene            1276..1482
                     /locus_tag="VFQ10_12070"
     CDS             1276..1482
                     /locus_tag="VFQ10_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966123.1"
                     /translation="MADTDSMLRYDVEPIHPVEAEIPGEGELSFAVFSLEHAACGDDV
                     VEVRIGERMLLEWCMACAVMEVFA"
     gene            1739..2845
                     /locus_tag="VFQ10_12075"
     CDS             1739..2845
                     /locus_tag="VFQ10_12075"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HET9966124.1"
                     /translation="MAELKRRALEVAQGVDERFDAFRFGLKRRLGLLDPFEILPYRGY
                     GTSRELFVRGRVLEEAGITRAGRDDAVWKNILNMARRFASDEVAGARVMASFGGLQVE
                     TTADAEGFFEVRFQLEEPLDGPGGWYQVELELLSPPSPGGGRVRSIGHVLVPRGARFG
                     VISDLDDTVVRSSATNVLKMAWIVVRNNAHTRLPFEGVAAFYRALQVGPDSRSSNPVF
                     YVSSSPWNIYDMLVDFLNVHGVPHGPLFLKDWSLSVLGKHRDYKMGVIRRLLRIYEDL
                     PFVLIGDSGEEDPEIYLQSVREHQGRIKAVYIRDVTSVERDAEVRAMANEARKLGTEM
                     VAVPDTTAAAEHAASIGLIAPNAIAAVQSESAGF"
BASE COUNT          558 a          835 c         1009 g          542 t
ORIGIN      
        1 gcggcggtgg gggtggcccg tacatggagg caagggacgc gctaacggcg ctcgggtacg
       61 gactcgagga ggccgagaag gccctcaacg acgtgccacc gcaggattcc gtggagaagt
      121 atataaagga ggcattacgg cagataggga gcaggcgttg aacactgagg agatggacga
      181 ctttaccgtg cggggcgaag gaagaaccga ggagctgcct gaagacatta ctggggctgc
      241 gggtttcggt gaggatccgc tcgaccccga ggcctacgca gaggacgatg agccctcgct
      301 caggccgagc tcgttcgacg agttcgtcgg gcaggaggcg ctcaaacaga acctcaagat
      361 cttcgtgcag gctgccaggc agcgcgatga ggccctggac cacatactgc tggacggccc
      421 tcccggacta ggtaagacca ccctctgccg tatcctcgcc gaggagatgg gggtcgggct
      481 caggccgacg agcgggccca ctttggagcg ggccggagat atggcggcga tcctgacttc
      541 actggaggaa cgcgacttcc tgtttatcga tgagatccat cgcctgaaca ggcagatcga
      601 ggaggtcctc taccctgcga tggaggactt cgcgatggac atcgtcctcg gccaggggcc
      661 ttcggcgcgc accatcagga tggacctgcc gcgctttacg ctggttggcg cgaccaccag
      721 gaagggcctg ctaacaaaac ctctgctcga ccgattcggt gtcgcggaga gactcgaata
      781 ctacgggccc agggacctgg agaagatcgt ggtccgcaac gcgaagatac tcggcgtacc
      841 catcaccgaa ggcggcgcca ggcaactcgc gcgccgcagc cgcgggacgc cccgcatcgt
      901 caatcggctc ctcaagcggg tgcgcgacta cgcccaggtc gtcggcgacg ggaagataga
      961 tgaagagacg gcgaacgccg cgctcgacat gcagggcgtc gaccacctcg gcctggacag
     1021 ggccgaccgt gtctatctcg ggcttgtcat agacaagttc gagggcgggc ctgtcggcat
     1081 cggtacgatc tccgtggccc tcggcgaggc gcgcgatacc gtcgaggatg tctacgagcc
     1141 ctacctgctg cagagcggct tgatccaacg caccccccgg ggacgggtcg ccacccagag
     1201 ggcctacggg cacctcggcg tcccggtccc gaacaacggc tagtcaccgg ccggtcgcta
     1261 aggtaaggcc gtgtggtggc cgatactgat agcatgctgc gctacgacgt agaaccgatc
     1321 caccctgtgg aggccgagat tccaggtgaa ggagagctct cttttgccgt tttcagcctg
     1381 gaacatgctg cctgcgggga tgacgtggtg gaggtgcgta tcggtgagcg aatgctactg
     1441 gagtggtgca tggcgtgcgc cgtcatggag gttttcgcct aaagcctctc gcccgttgcc
     1501 gaaggcgcga ttcgcactac gtcaatacct gtgcaaatcg ccttggtaac cgaggccttg
     1561 tctaatgttc gcccgccgtg ttagtttccg tactacagga taggttgatc caggtttagt
     1621 gggggcaaga tggccgaccg gggtggtgcg aacgacgccg gcacggactt gcaattgttc
     1681 gggatcgtaa cgccgaggtg ggaagaggtt ctcccgggct agtcggaagg gaaactcgtt
     1741 ggccgagttg aagcggaggg cgctggaggt cgcgcagggg gtcgatgaac gcttcgacgc
     1801 cttcaggttc gggctgaagc ggcgtctcgg gctgctagat ccgttcgaga tcctacctta
     1861 ccggggctac ggcacgagca gggagctgtt cgtgcggggc cgcgtcctgg aggaggctgg
     1921 catcacccgc gccggtcggg acgatgcggt atggaagaac atactcaaca tggcgcggcg
     1981 cttcgcgagc gatgaagtgg ccggcgcgcg ggtgatggct tctttcgggg gtttgcaagt
     2041 cgagaccacc gccgacgcgg agggcttctt cgaggtgcgc ttccagcttg aggagccgct
     2101 cgacggtccg ggcgggtggt accaggtcga gttggagctc ctgtctccac cgtcgccggg
     2161 tggaggaagg gtgagatcga taggacacgt gctcgtgccg cgcggagccc gtttcggtgt
     2221 gatcagcgac ctcgacgata cagtggtgcg ttccagcgcg acgaatgtgc tgaagatggc
     2281 ctggatagtg gtacgcaaca acgcccatac gcggctacct ttcgagggcg tcgccgcttt
     2341 ttacagggct ttgcaagtgg gcccggacag cagatcttcc aatcctgtct tctacgtgtc
     2401 tagcagtccc tggaacatct acgacatgct tgtggacttc ttgaacgtgc atggcgttcc
     2461 ccatggaccg ctctttctca aggactggag cctgagcgtt ctcgggaagc acagagacta
     2521 caagatgggg gtgatccggc gcctcctccg catctatgag gacctgcctt tcgtcctgat
     2581 aggggactcg ggggaggagg acccggagat ctatttgcag tccgtgcggg agcaccaggg
     2641 acgcatcaag gccgtttaca tcagggacgt cacttcggtc gagcgtgacg ccgaggtcag
     2701 agcgatggcc aacgaggcgc gcaagctcgg gacggagatg gtggcggttc ccgataccac
     2761 cgcagcggct gagcacgcgg cctccatagg tctcatagcg cctaacgcga tagccgcggt
     2821 ccagtctgag agcgccggat tctagccctt gacccgctgt aatcggagga tgacatttct
     2881 tggagtgcgg gctgttctgt caagaaccga tgccgaatcg ggcctctact tcgggtatga
     2941 tctc
//
LOCUS       DASTKM010000303         6111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_654027, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000303 DASTKM010000000
VERSION     DASTKM010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654027"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /gene="xylB"
                     /locus_tag="VFQ10_12080"
     CDS             complement(<1..1481)
                     /gene="xylB"
                     /locus_tag="VFQ10_12080"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HET9966125.1"
                     /translation="MSVQIGLDVGTGGARAVAVDESGEIVAEASSEYPLHSPRPGWTE
                     QDPDDWWEGAKEVIKKVATETREQVAGIGLTGQMHGSVFLDSSDEVIRPALLWNDQRT
                     HRQCEEITRKVGEERLISISGNPALTGFQAPKILWLREEEPDNFGRVSRVLLPKDYVR
                     LRLTGEYATDASDAAGTLLLEMRARDWSGEILDHLEIPLEWMPEVYEGPESTGALRKS
                     VAEELGLPQGIHVAAGGGDNAAAAVGTGIVGPGLVNSSVGTSGVLFAHAGEFEPDPSG
                     RLHAFCHAVPGAYHLMGVTLSAGGSLSWWRETLGGDYDELVEAASGVRPGSEGLLFLP
                     YLSGERTPHLDPKARGAFFGLTARHGVEHMTRAVMEGVIFSLRESLEIMRGLDVPVED
                     VRATGGGARSDLWRQLQADIYGTPIRRTVADEGPAYGAALLAGAASGTYADVHQAVSV
                     VKLREEITDPDTERANIYEEHYEIYRSLYPATSTAMSRLTDLAA"
     gene            complement(1478..2557)
                     /locus_tag="VFQ10_12085"
     CDS             complement(1478..2557)
                     /locus_tag="VFQ10_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET9966126.1"
                     /translation="MIKNSVGIWAFGPAVTRFVPPGYHNEVADEPMVEKTERVAGGLH
                     DLLEGLEYHYPGEINEENVEEIMPVLAAYGMDLPVIAAGLHPDPTYALGALINPDEGL
                     RRKGIDTLSRGIDLCAQVGANFIIWPGAEGYNYTFQRPYAEIWRRFVEGVAELTDHAN
                     EKGVKVFLEHKNSEPAMKILMQNIGMTLFTIQKVNTLGVDTTNLQVNMDWQHLIMNGE
                     NLAEYADLLASEHKLGHQHGNDGWGTFDDDNVVGTNFFMQTLELAQTLQDTGYGEEGE
                     IMGFDLYPYTEDQVAAVRRAILQWEFVWDLARKIDRTGLREAKTRADALAGQKAVYAA
                     LGMDEGYEAEISSRRKEARDGRGET"
     gene            2733..3503
                     /locus_tag="VFQ10_12090"
     CDS             2733..3503
                     /locus_tag="VFQ10_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9966127.1"
                     /translation="MELGLDNKTFVVGGASSGLGRAVAEQLVAEGARVLLVARNEDAI
                     QEAARELGEQAEQCIADVSQPSGVDKVAAVAVDRFGTLDGVLVNAGGPPFGPALELTD
                     EQWLHAFRLLLGAPIRLLRALVPQMNDDASVLFITSSSVREPIQNLDASNVLRPGVAA
                     LVKCLAKELGPRVRVNSMAPGRIDTARSRSLDEARAQDSGIAPEEQRRNVSEGIPLGR
                     YGEPAELGRAAAFLLSPAASYITGVSLQVDGGLVSAVP"
     gene            3742..4965
                     /locus_tag="VFQ10_12095"
     CDS             3742..4965
                     /locus_tag="VFQ10_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET9966128.1"
                     /translation="MIFEVSEKTREFREQVEAFMDELVYPNEAKYHEQLEASGSRWSV
                     PQVMEELKAEAKARGLWNLFLPGSDLGAGLTNLEYAPLCEIMGRSPIAPEAFNCNAPD
                     TGNMEVLVRYGTEEQKERWLRPLLDGEIRSCFAMTEPDVASSDATNIRARIERDGDEY
                     VVNGRKWWISGAGDPRCRISIFMGKTDPDAPRHEQQSMILVPLDTPGVEVERTLPVFG
                     YDDAPHGHAEISFEDVRVPLENIIWDEGKGFAIAQGRLGPGRIHHCMRLIGVAERALE
                     MMCERVKGRETFGKPLAGQGVVQAWISYSRMEIEQARLLTLKAAHMMDTVGNKEARAE
                     IAMIKVVAPNVALNVLDRAIQAHGGAGVSDDFPLAYFWASARTLRLADGPDEVHRAAV
                     ARLELKKERRHRTES"
     gene            5000..5245
                     /locus_tag="VFQ10_12100"
     CDS             5000..5245
                     /locus_tag="VFQ10_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966129.1"
                     /translation="MLLGIKYEAARIGDVGEDALAGIEEQIKREVGDGWKSDDRVLRG
                     RNAVRREHGLPGLGEPGDRPSPATDPIRPLWRRLFGR"
     gene            complement(5442..5828)
                     /locus_tag="VFQ10_12105"
     CDS             complement(5442..5828)
                     /locus_tag="VFQ10_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966130.1"
                     /translation="MWLKQIAEIDLKQECLLDLRLDGDITPEQFRARSKELKEARVAA
                     QDQLEASRSRLSRLKDLERSKDTLVSHYASLVPVGLNELRPADRNKVCEMMHLRVFAH
                     PDDTLIVEWGCNVSPLPLDSSRTRGR"
BASE COUNT         1120 a         1875 c         1960 g         1156 t
ORIGIN      
        1 gcggcgaggt ccgtgaggcg ggacatggca gtactcgtcg ccgggtagag cgagcggtag
       61 atttcgtagt gctcctcgta gatattcgcc cgctctgtgt cgggatcggt gatctcctct
      121 cgcagtttca ccacggaaac agcctggtgc acgtcggcgt aggtgcctga ggccgcccct
      181 gccaggagtg cggcgccgta ggccggtccc tcgtcggcga cggtcctgcg tatgggggtg
      241 ccgtagatgt ctgcctggag ctgacgccac agatcgctcc tcgcccctcc gccggtagcc
      301 ctcacatctt ctaccggcac atcgagtccg cgcatgatct ccagagattc gcgcagggag
      361 aagatcacac cctccatcac ggcgcgggtc atgtgctcga cgccgtgacg ggccgtgagc
      421 ccgaagaaag cccccctggc tttggggtcg aggtgcggcg tccgctcgcc agagaggtaa
      481 ggcagaaata ggagcccttc tgagcccggc ctgacgccag aagcagcctc taccagctcg
      541 tcgtagtcgc ccccgagcgt ctcacgccac caggacaggg accctcccgc agagagggtg
      601 acgcccatca aatgatatgc gccgggcacg gcgtggcaga aggcatgcag gcgccccgat
      661 gggtcgggct cgaactcgcc ggcgtgggcg aagaggacgc cgctggtacc gacggaggag
      721 ttgacgagcc cggggccaac gatgcccgtg ccgacggcag ccgccgcgtt gtcgccgccg
      781 cccgcggcca cgtggatgcc ctgtggcagg ccgagctctt cggccacgct cttgcgaagg
      841 gcacccgtgc tctcggggcc ctcgtagact tcgggcatcc attcaagtgg gatctccaga
      901 tggtccagga tctcacccga ccagtcgcgg gccctcatct cgaggagcag ggtgccagct
      961 gcatcagagg cgtctgtggc gtactcgccc gtgagcctta gcctcacgta gtccttgggc
     1021 aggagcacgc gggagacccg cccgaagtta tccggctctt cttctcttag ccagaggatc
     1081 ttcggtgcct ggaagcccgt gagggccggg ttgccggaga tggagatcaa gcgctcctct
     1141 cccactttcc tggttatctc ttcgcactgc ctgtgggtac gctggtcgtt ccacaacagc
     1201 gcaggcctta ttacttcatc agatgaatcg aggaacacgg agccgtgcat ctgcccggtg
     1261 agcccgattc ctgccacctg ctctcttgtc tctgtggcga ccttctttat gacctctttg
     1321 gcaccctccc accagtcgtc tgggtcctgc tcggtccagc cagggcgtgg gctgtgaaga
     1381 gggtactccg aggaggcttc ggcgactatt tctcccgact cgtccacggc gacggccctt
     1441 gcgccgccgg tcccgacgtc gaggccgatt tgaacactca cgtctctccc ctgccgtcac
     1501 gcgcctcttt gcggcggctg gagatctccg cctcataacc ttcgtccatg ccgagcgctg
     1561 cgtagaccgc cttctggccg gccagggcgt cagcacgggt cttggcctcc cgaagcccgg
     1621 ttctgtctat cttccgcgct aggtcccaga cgaactccca ctgcaggatg gcccgcctga
     1681 cggcggccac ctggtcttcc gtatagggat agaggtcgaa gcccatgatc tccccttctt
     1741 ccccgtagcc cgtgtcctgg agcgtctggg ccagctcgag ggtctgcatg aagaagttgg
     1801 tgccgaccac gttgtcgtcg tcgaaggtgc cccagccgtc gttgccgtgc tggtggccga
     1861 gcttgtgctc cgaggccaag aggtcggcgt actcggccag gttctcgccg ttcattatga
     1921 ggtgctgcca gtccatgttg acctgcagat tggtcgtgtc caccccgagg gtgttgacct
     1981 tctggatcgt gaagagcgtc ataccgatgt tctgcatcag gatcttcata gcgggctctg
     2041 agttcttgtg ctccagaaag accttgacgc ccttctcgtt ggcgtggtcg gtcaactcgg
     2101 cgacaccctc gacaaagcga cgccagatct ccgcgtaagg acgctggaaa gtgtaattgt
     2161 agccctcagc gccaggccag atgatgaagt tcgctcctac ttgcgcgcag aggtctatgc
     2221 cgcgactcag ggtatctatg cccttgcgac ggagcccctc gtcggggttt attaacgcgc
     2281 ccagtgcgta ggtcgggtcg gggtggaggc cagcggcgat cacgggcagg tccatcccgt
     2341 aggcggcgag gacaggcatg atctcctcga cgttctcttc attgatctca ccaggatagt
     2401 ggtactcgag cccttccagg aggtcgtgga gccctccggc gacgcgctct gtcttctcga
     2461 ccataggctc gtccgcgacc tcgttgtggt agcccggcgg cacgaagcgg gtcacagcag
     2521 ggccgaaagc ccatatccca acagagttct tgatcatctg gctcctcctt gcctctaatc
     2581 tacaagagac gcgctgttac ccccgtatca tcatagcccg aaccttgcaa gcatttcagg
     2641 gtccgatctt tactttcagc ccatcgccct catggcatca cgagagggtg taggattgtc
     2701 ctgggcggcg ttcttagggt aaggaggcga ccgtggaact aggactggac aacaagacct
     2761 ttgtggtagg cggcgctagc agcgggctcg gcagggcggt tgccgagcag ctagttgcag
     2821 aaggtgccag ggttctcctg gtcgcacgca atgaggacgc cattcaggag gcggcgcgag
     2881 agctcggcga gcaggccgag cagtgtatag cagacgtctc ccaaccgtcg ggcgtagaca
     2941 aggttgcagc ggtcgcggtc gaccgcttcg gcacgctcga cggcgtgctc gtcaacgccg
     3001 gtggcccgcc cttcgggcca gcgctcgagc tgaccgatga gcagtggctc cacgccttcc
     3061 gtctgctctt gggcgcgccc atacggctac tgcgcgccct cgtcccgcag atgaacgacg
     3121 acgcctccgt gctcttcatc acctcatcat cggtgcgcga gccgatacag aacctcgacg
     3181 cctcgaacgt gctgcgcccc ggggtggcgg ccctcgtcaa gtgcctggcg aaagaacttg
     3241 ggcccagggt gagggtcaac agcatggccc cagggcgcat cgacaccgcc cgctcccgct
     3301 cgctcgacga ggcgcgggcc caggactccg gtatcgcgcc cgaggagcaa cgccgcaacg
     3361 tgagcgaggg aatccccctg ggccgctacg gtgagcctgc ggagctaggg agggcggcag
     3421 ccttcctact ctccccggcc gcctcataca taaccggcgt ctccctacaa gtcgacggcg
     3481 ggctcgtctc cgccgtacct tgatgcagca ggttacagag ttcaggccaa cctgaacggc
     3541 ggcgaaaaga acctgggtga catccttgat ggcctggaca tccccgccta cgacactatt
     3601 tgcggctaga gacagacttt tccgaagagc gttccgcccc atcacgacga ccgattctac
     3661 ggggctgaga acccggggcg attgcgacgc ctatgatgct gtgccagaat cggctctccg
     3721 gctgttgtga gaggagaggg cgtgatcttc gaggtttccg agaagacaag ggagtttcgg
     3781 gagcaggtcg aggcgttcat ggacgagctc gtctacccga acgaggcgaa gtaccatgag
     3841 cagttggagg cgtcagggag ccggtggtcc gtaccccagg tcatggagga actcaaggcg
     3901 gaggcgaagg cgagggggct gtggaacctc tttctgcccg gctcggatct cggtgccggc
     3961 cttaccaacc tggagtatgc acctttgtgc gagatcatgg gccgcagccc gatagccccc
     4021 gaggctttca actgcaacgc ccccgacacg ggcaacatgg aggtgctcgt acgctacggt
     4081 acagaggagc agaaagagcg ctggctaagg ccgttgctcg acggtgagat aaggtcctgc
     4141 ttcgcgatga ccgagccgga tgtggcctcc tccgatgcga cgaacatcag ggcacgcata
     4201 gagagggacg gagacgaata cgtggtgaac ggcaggaagt ggtggatctc tggtgccggt
     4261 gatccgcggt gcaggatctc catcttcatg ggcaagacgg accccgatgc gccgcgccac
     4321 gaacaacagt ccatgatcct ggtcccacta gatacgcctg gcgtcgaggt agagcgaacc
     4381 ctccccgtct tcggctacga cgatgctcca cacgggcacg cggagatctc cttcgaggac
     4441 gtgcgcgtgc cgctggagaa catcatctgg gacgaaggca aaggcttcgc catcgcccag
     4501 ggacgcctcg gtccggggcg catccaccac tgcatgaggc tcataggcgt tgcagagcgg
     4561 gcgctcgaga tgatgtgcga gcgggtcaaa gggcgggaga cattcgggaa gccgcttgcg
     4621 gggcagggtg tcgtgcaggc ctggatctcg tactccagga tggagataga gcaggcgcgc
     4681 cttttgacct tgaaagcggc ccacatgatg gatacggtag gcaacaaaga ggccagggcg
     4741 gagatcgcca tgatcaaagt cgtcgcaccc aacgtggccc tgaacgtgct cgaccgggct
     4801 atacaggccc acgggggcgc aggggtctcg gatgacttcc cgctcgccta tttctgggcg
     4861 agcgcccgca ccctgcgcct cgccgacggc cccgacgagg tccaccgcgc cgcggttgcc
     4921 aggctcgaac tcaaaaaaga gcgtcggcac aggacagagt cataaagtag aggaggagaa
     4981 catcgatagc aagaactccg tgctcctcgg catcaagtac gaggccgcga gaataggcga
     5041 cgtgggtgaa gacgcgctcg ccggtatcga ggagcagatt aaaagagagg tcggcgacgg
     5101 ctggaagtcg gacgaccggg tactcagggg acgcaacgcc gtaaggcggg agcacggact
     5161 accagggctc ggcgagccag gcgacaggcc ttccccggcc acagacccaa tacgcccgct
     5221 ctggcgcagg ctatttggga ggtagcccca cggtggctat aagtgggccg gcacgagcga
     5281 ggagttcggc gcgatggcga tagagatgac tggtggcaag atcgtcaacg aacgcggcac
     5341 ggtcgttacc ttcaggcagc ggtgcgaggc gtgcggctat gtcttcgact ggaacaagac
     5401 taccatcgtt cctgcatacg gctctcgaaa ggtacgacct ttcacctgcc ccgagtgcgg
     5461 gaactatcaa gaggtagagg cgagacatta caaccccact cgacaatgag ggtatcgtcg
     5521 gggtgcgcga agactcgcaa gtgcatcatc tcgcagacct tgtttctatc tgccgggcgg
     5581 agttcgttca gtcccacggg aacgagggag gcgtagtgtg agacgagggt atctttgctg
     5641 cgttcgagat ctttgaggcg agacaggcgt gagcgggagg cttctagctg atcctgcgcc
     5701 gcaacgcggg cctccttgag ctctttgctc ctggcgcgaa actgctcggg cgttatgtct
     5761 ccgtcaaggc gcaggtcgag caggcactcc tgcttgaggt ctatctcggc tatctgcttt
     5821 agccacaaag cctcatcctc tgccgtagat ggggcacgct cgttccctct tcgggccaga
     5881 gtcccaattg agggctccgg ttccttcgtg ttacggggtg ggtcgcggag ataggcggtt
     5941 gcattgctgg gcgtgcatac cccctcgaaa aatccgcgcc ggtttcagga atgtaagcat
     6001 acatcgctta tcttcgcgat aggtaaattc ttagaagttc gcatggtgcg cgtaccccga
     6061 agctctgcat agccccggtc ctatgtggca gggtttctta tgtgctatcg g
//
LOCUS       DASTKM010000304         3924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1535422, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000304 DASTKM010000000
VERSION     DASTKM010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535422"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFQ10_12110"
     CDS             complement(<1..254)
                     /locus_tag="VFQ10_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966131.1"
                     /translation="MKVSTFLTEVLAIARRSTVRTLRQPAVLVPALLFPMLFFSINAN
                     GLDAASKIPGFPTDSYLDFAFAFPLIQASLFGAITAGADLA"
     gene            complement(251..1240)
                     /locus_tag="VFQ10_12115"
     CDS             complement(251..1240)
                     /locus_tag="VFQ10_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9966132.1"
                     /translation="MGRERGIEAENLVREFRKGPRAVDGIGLRVEPGEVYGFLGPNGA
                     GKSTTVLMLTTLLPPTSGTATVAGYDIVREGPQVRASIGAALQEAALDPYLTGREHLR
                     LQAALHGLSRKERKRRGQELLERVGLEEAADRKVRGYSGGMKRRLDLALALVHGPQIL
                     FLDEPTTGLDPQSRSALWAEVSRLARDEGVTVFLTTQYLEEADVLADRVGIIDRGKIV
                     AEDTPEALKAEIGRPSVEATPAERSERGAVVRVLREFGEEIPAQPGAVAVRLSSGAAG
                     LAEVVRALDRENVRVANLRLDEPSLDDVFLAKTGRSLEGAGEDEDGGMEEAQA"
     gene            1582..2709
                     /locus_tag="VFQ10_12120"
     CDS             1582..2709
                     /locus_tag="VFQ10_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966133.1"
                     /translation="MSAVRKITDLRALISDLSPEDRILAKRIFDVSTTTGRLDPPEAM
                     HAWIEKSFGSVDHVTAQRIVKVTNRITLEGTLFNGLRASRPLDTGVSLDLDREIAATK
                     GDPFCHPKEGTPADVFGRVEGEHATTASNVAKYDGFHGLIVFDEHNPLHLTPEKVAGY
                     VSVGLEWGRKALEVDSEAHYPFLMWNCLWRAGGSIIHGHAQITATRGSHYPKVERLRR
                     TASAYKREHASDYFEDLTRVHASLGLGIPLEGARAFASLSPIKEKELVVIGEDPGDAS
                     LGRAVGALLQGYVEDLGVRAFNVAFYMPPLGSTQEDWSDFPTVVHIVDRGDPSNRTSD
                     IGAMELYAASVVASDPFRVAVALRERGSGSTEGAARPAGTE"
     gene            complement(2857..3378)
                     /locus_tag="VFQ10_12125"
     CDS             complement(2857..3378)
                     /locus_tag="VFQ10_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET9966134.1"
                     /translation="MKFWKIVVLALLLAGCGGPQADPDTQSQRTPHSSATTAAGKEET
                     TSGPSGLRTLVIDASGGKKVEVRVEVADDLAEQAKGLMYRTTLGENRGMFFVYPDERE
                     RSFWMKDTLIPLSIAFIDSERLIIDIQDMKPLDDEPPHYVSAEPAQYALEVNQGFFEK
                     GGVKVGDRVDVSQ"
     gene            complement(3375..>3924)
                     /locus_tag="VFQ10_12130"
     CDS             complement(3375..>3924)
                     /locus_tag="VFQ10_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HET9966135.1"
                     /translation="AMIVDVRREGGEVVHVMDRPIPDTVGDLKGALEWERRFAHMRYH
                     TALHVLSGVIWRNFDARVTGGQMRADRARMDFSFPGEWTAGVVGEIERLTNDALAEER
                     PVRVYELEREEALANPDLIRTQVSLVPERVRRIRIVEIQGLDTQADGGTHVANTREVG
                     RIEITGHKSKGRQNKRIEFVLR"
BASE COUNT          689 a         1332 c         1186 g          717 t
ORIGIN      
        1 gccaggtcgg cgccggctgt gatagcgccg aatagggacg cctgtatgag agggaaagcg
       61 aacgcgaagt cgaggtaaga gtcggtgggg aaaccgggga tcttcgaggc ggcgtcgagg
      121 ccgtttgcgt tgatcgagaa gaataacatc ggaaagagta gcgcagggac gaggacagcg
      181 ggctgcctca gggtacgaac cgtggagcgg cgcgcaatcg ccagtacttc ggtaaggaaa
      241 gtcgagacct tcacgcctgc gcctcctcca taccaccatc ttcgtcctcc ccggcgcctt
      301 ccagcgagcg acccgtcttg gcgaggaaga cgtcgtcgag gctgggctcg tcgagacgga
      361 gattggcgac gcggacgttc tctctatcca gggcgcgcac cacttcggcg aggccggcgg
      421 caccgctgga gagcctcacg gccacggcgc ctggttgggc cgggatctcc tctccgaact
      481 cgcgaagtac gcgtactacg gcgccccgct ccgagcgctc ggcgggcgtc gcctcgacgc
      541 tggggcgtcc gatctcggcc ttcagggcct ccggcgtgtc ctcggcgacg atcttgcccc
      601 ggtcgatgat cccgacccgg tcagcaagta cgtcggcctc ctcgaggtac tgggtcgtca
      661 ggaacacggt gacgccctca tcgcgcgcga gcctgctgac ctcggcccag agcgcgctgc
      721 ggctctgcgg atcgaggccg gtcgtcggct cgtcgaggaa gagtatctgc ggaccgtgca
      781 ccagcgcgag ggcgaggtcg aggcggcgct tcatcccgcc cgagtagccg cgtaccttcc
      841 tgtctgcagc ctcttcgagc ccgacgcgct ccagtagctc ctgcccacgc cgcttccgct
      901 ctttacgcga gagcccatgc agtgccgcct ggaggcgaag gtgctcgcgg ccagtcaggt
      961 aaggatctag cgccgcctcc tggagcgccg cgccgataga ggcccgcacc tgcggtccct
     1021 cgcgtacaat gtcgtagccg gccacggtag ccgtgccgct cgtgggcgga aggagcgtcg
     1081 tcagcatcag aacggtcgtt gacttccccg ccccgttcgg accgaggaag ccgtagacct
     1141 cacccggctc cacacgcagg ccaatcccat ccacggcccg cggtcccttc ctgaactccc
     1201 tcacgaggtt ctcggcctct atcccgcgct cccggcccac taggcggcct tcctgatacc
     1261 ggaggcgccg cccgtgatga ggactgtatt acctcccata ccaccgttcg aattatccaa
     1321 gacctctcct gcactagaaa actcgcatca ccctgagtct ataccgcttt cgcgtccgag
     1381 caccgtgagc ggagccacgc actacccgcc cgtctctgtg gtcgtgtagt gttcccgatc
     1441 ccaccttcgt gggtggacgt aaggccggaa ggttgactag actgcatggc tatgagggtc
     1501 gagggacacg tggagcgtat gatcgaccgc gagagcctct acgcaggcct gaaatccgcc
     1561 ggacagcagg acgtttagtc gatgtcggcc gtacgcaaga taactgacct tcgggccctg
     1621 atctccgacc tctcccccga ggatcgcatc ctcgcaaagc gcatcttcga cgtctccacg
     1681 acgacgggac gcctcgaccc gcctgaggcg atgcacgcct ggatcgagaa gtccttcggc
     1741 tcggtggacc acgtcaccgc ccagcgcatc gtgaaggtca cgaaccgcat caccctggaa
     1801 ggcacactct tcaacggtct gcgcgcctcc cgcccactcg atacgggcgt cagcctggac
     1861 ctcgaccgcg agatagcggc gaccaaggga gacccattct gccacccaaa ggaaggcacg
     1921 ccggccgacg tcttcggccg tgtggagggt gagcacgcta ccacggcttc gaacgtcgcc
     1981 aaatacgacg gcttccacgg cctcatcgtc ttcgacgagc acaaccccct ccacctgacg
     2041 cccgagaagg tcgctggcta cgtctccgtc ggcctggaat ggggccgcaa ggccctcgaa
     2101 gtcgactccg aggcgcacta ccccttcctt atgtggaact gtctctggcg ggcaggcggc
     2161 tccatcatcc acggccacgc acagatcact gcaaccaggg ggtcccacta cccgaaggtc
     2221 gaacgcctcc gccgcaccgc ttcggcctac aaacgggaac acgcctccga ctacttcgag
     2281 gatcttaccc gtgtccacgc ttctctcggc ctgggcatcc cgttggaagg cgcccgcgcg
     2341 ttcgcgagcc tctcccccat caaggagaag gagttggtcg ttatcgggga ggatcctgga
     2401 gacgcttccc tgggccgcgc ggtcggcgcc ctgttgcaag gctacgtcga agacctcggc
     2461 gtccgcgctt tcaacgtcgc cttctacatg ccccctctcg gttccacaca ggaggactgg
     2521 tcagactttc caacggtcgt ccacatcgtg gatcggggcg acccgtccaa ccgtacctcg
     2581 gacatagggg cgatggaact ctacgcagct tccgtcgtcg cctctgaccc tttccgggtc
     2641 gccgtagcgc tgcgtgagcg aggatctggc tccaccgaag gagccgcacg gccagccggt
     2701 acggaatgac cttccagcgc gatcggttgg aggagatcat gcggcaacac gacctcaggg
     2761 tgcaatagca gagcatgcag caacgcaagg gttaggctac gaggccacat ccgcgtctgc
     2821 agcgagtcga gcgatccgcc cctgatccat ggggccctat tgggacacgt cgacgcggtc
     2881 ccctactttc acgccccctt tctcgaagaa gccctggttt acttcgagag cgtactgggc
     2941 gggctcagcg gagacgtagt ggggcggttc atcgtcaagg ggtttcatat cttggatgtc
     3001 tatgataagc ctctccgagt cgatgaaggc aatggagagc ggtatcaggg tgtccttcat
     3061 ccagaaagag cgctcccgct catcggggta gacgaagaac atgcccctgt tctcccctag
     3121 cgtcgtccta tacatgagac ccttagcctg ttcggccagg tcatcggcca cctcgacccg
     3181 cacctcgacc ttcttccctc ccgaagcgtc gataaccagt gtgcggagcc ccgatggtcc
     3241 cgaggtcgtc tcttctttcc ccgcggcggt agtggcggaa ctatgagggg ttctctggga
     3301 ttgtgtgtca gggtcggcct gaggaccacc gcagccggcc aggagcaggg cgaggacgac
     3361 gatcttccag aatttcaccg gagcacgaac tctatccgtt tgttctggcg gcccttgctc
     3421 ttgtggcctg tgatctctat cctcccgacc tcgcgcgtgt tggcgacgtg ggtcccaccg
     3481 tcggcctgcg tgtcgaggcc ctgtatctcc acgatccgaa tacgccgcac ccgctcaggc
     3541 accaggctga cctgggtacg gatgaggtcg gggttcgcga gcgcttcttc gcgctccagc
     3601 tcatagaccc ttacaggacg ttcttcggcg agagcgtcgt tggtcaggcg ttcgatctca
     3661 ccgactacgc cggcggtcca ctcgccgggg aaagagaagt ccatcctggc gcggtctgcg
     3721 cgcatctggc cacccgttac cctggcgtcg aagttgcgcc agatcacacc cgagaggacg
     3781 tgcagcgccg tgtggtatcg catgtgggcg aatcgccgct cccattccag cgcgcctttc
     3841 agatccccca cggtatcagg gatgggtctg tccataacgt gcacgacctc tccaccctct
     3901 cgcctcacgt ccacgatcat agcc
//
LOCUS       DASTKM010000305         5116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1535578, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000305 DASTKM010000000
VERSION     DASTKM010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535578"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..964
                     /locus_tag="VFQ10_12135"
     CDS             212..964
                     /locus_tag="VFQ10_12135"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HET9966136.1"
                     /translation="MMGLRNLYKVFRETRNAAREQATLAVTGDSPRAAEVAALLGAQR
                     GVRGAEVILTVSETGATISGKAVEDPGEITLPSKDGKAMLEELALRIVGALDEDYLVA
                     LAKGYPPLRRAVCEEIVRNNARQNAVIGALPIPGADMPVMTANQARMVLNIAAAYGEE
                     LSMDRARELLGVLAAGFGLRALSRQAVKLVPVAGWAASAVIAYAGTVAMGRTAILYFE
                     RGKQEIGAEEMARIRSRAIEEARAFVSRMRRK"
     gene            1038..2318
                     /locus_tag="VFQ10_12140"
     CDS             1038..2318
                     /locus_tag="VFQ10_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HET9966137.1"
                     /translation="MFSLLRRALSETRSWLATLRRLGLRVFLALTALQAVIVGVLVAT
                     AEFRKRRQGPREGFPWEDQPEIRMESGEDRLKIYAYGVNLYEAMLDEIEAAQDEIFIG
                     TFIWKADEIGRRFVDALARKAGQGVEVYVVFDGLANVLVPPSFKRFPAKIHRLHFRPL
                     SGPAKILNPRNVFRFHRHVMSVDGKVAFLGGYNIGTLYAAGWRDTHVRVRGDVAREVD
                     NAFVDFWNAHRTEELPRITPNKERDWNPAVRVRRNDPYMRIFPIREMYIEAIDRANSH
                     VYLTHAYFVPDRALKAHLIDATKRGVDVQLLVPEQSNHVTADWLARRHFYDLLKNGVR
                     IFLYRHIMIHSKTATADGLWSTIGSANIDRLSMLGNYELNIEVYSSGLAAQMERMFEM
                     DKTNAEEVTLEEWERRPLHAKVVERTLTSLSPLV"
     gene            2435..4099
                     /locus_tag="VFQ10_12145"
     CDS             2435..4099
                     /locus_tag="VFQ10_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET9966138.1"
                     /translation="MARWRLQEPDAEMVSELAREADVDILCARVLANRGVTPGEAADF
                     LSPSLAAIGMPDEDQWNVAAERVAKAIAGGERIGIFGDYDTDGITSAAVMFLALSHYT
                     ENVTVRLPTRQTGYGLLEPYVRDLFAEGVDLLITADCGISNRSEVALANELGMEVVVT
                     DHHIPPEDPPDTAVAVLDPKLWNPEDPLAGVGVAWKFAWAVARKLGDAGGRKRLGRLL
                     DLVSVGTVVDIAPLVGDNRALASMGLKHMNRGLAAGGARPGIEALVKVAGVRGEIDED
                     DLGWKLGPRINSIGRIKNPRPALDLLLTDDRREAFRIASLLNQLNAERQRRTRSAVER
                     AMAEVDPEQDFKVVVTEETGGIAGLIAGRVAGATGRPAAILTRRADDSYGGSARAGET
                     DVDLYGALYAVRHLMGEWGGHRRAAGLSVNPGNLDAFVAGVNEAVRAQSEQKPEIFVP
                     AIEVDAEIPLGSVSNGLLDWHEMLAPFGSGNYRPIFVTEDLRIAGSRKIWEGMNLLHL
                     EGGIKAKMAGEPDGLPSGPFDAAYTVTRSRYSGQAELEIVDWKAGR"
     gene            4096..5049
                     /locus_tag="VFQ10_12150"
     CDS             4096..5049
                     /locus_tag="VFQ10_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9966139.1"
                     /translation="MNAVGVDVGGTKIAAGVVTPEGKILDEVRYPTPHSPETLVETIA
                     RAISEVGDGPQVGGVCLAVPGLILAQANKVVFSPNLRAVEGIPLKDELEPKIGMPLTI
                     ENDGNAAAWGEFRFGVGSEADHLVFVALGTGIGGGIITHGFLMRGAQGSGGELGHVTI
                     HATGPRCACGNHGCLEALASGTAIGRRARELAVENPDSALGRLATRRQILGEDVTELA
                     REGDEVALSVLDEAGRWLGVGLAGFVNIFNPEVVAVGGGVMEAGELILRAARREVILR
                     ARPPSRDLADVKVATLGPRSGVLGAAALARDASSGEYLLGG"
BASE COUNT          950 a         1559 c         1725 g          882 t
ORIGIN      
        1 tccagcgtgc cggtctcctg accggcctcg aagagctcct gtatctcctc tatgtcccgc
       61 aggtcacgtt gaggctgcaa aatctactcc gggaacttca ggttgaaacc ctcctgtagc
      121 cttcacgtaa tatatcttaa acgagacaat aatcaatttg cttccgcgca caggcggagt
      181 cgtgcgggca agaccacaag gaggacaagc catgatgggc ctgagaaatc tatacaaggt
      241 gttccgcgag acccgcaacg cggctaggga gcaggctacc ctcgccgtca ccggcgactc
      301 gccaagggcc gccgaggtcg ccgcccttct aggggcgcag agaggcgtgc gcggagccga
      361 agtgattctc actgtctcgg agacgggggc tacgatctcg gggaaggccg tcgaggaccc
      421 tggtgagata acccttccct cgaaggacgg gaaggccatg ctcgaagagc tggcgctgcg
      481 catagtgggg gcgctcgacg aagactatct ggtcgcgctc gcgaaggggt atcctccgct
      541 caggcgggcc gtttgtgagg agatagtacg caacaatgcc cgccagaacg ccgtgatcgg
      601 tgcgctaccc atcccagggg ccgacatgcc ggtgatgacg gcaaaccagg cgcggatggt
      661 gctcaacata gcggctgcct acggcgagga actgtcgatg gatagagcgc gggagctcct
      721 gggggtactc gcggccgggt tcggactgag ggccctgagc cgccaggccg tgaagctcgt
      781 gcccgtggcg ggatgggccg cctcggcggt tatcgcctac gcaggtaccg tggcgatggg
      841 ccgtacagcc atcctctact tcgaacgcgg caagcaggag ataggcgcag aggagatggc
      901 caggatcagg agccgagcca tcgaggaggc cagggccttc gtctcgcgca tgcgtcgaaa
      961 gtaggtatca ggttgatctt cgagggccct tgcgggaagg tcggcccttt cgccgagcgc
     1021 tctatataat cgaatccatg ttctctctct tgcggcgggc actttcggag acgcgttcct
     1081 ggctcgcgac gttgcgcagg ctgggattga gggtctttct cgctttgaca gcgttgcagg
     1141 cggtgatcgt tggtgtgctc gtcgcgacgg cagagttccg caagcgccgc cagggtccgc
     1201 gggagggatt cccatgggag gaccagcccg agataagaat ggagtcgggc gaggatcggc
     1261 tgaagatcta cgcgtacggg gtcaacctct acgaggcgat gctcgacgag atcgaggctg
     1321 cgcaggacga gatcttcatc gggacgttca tctggaaagc tgatgagatc ggacgccgtt
     1381 ttgtggatgc cctggcccgt aaggcgggcc agggggtcga ggtttacgtc gtcttcgacg
     1441 ggttggccaa tgtgttggtg ccgccttcgt tcaagcggtt tcccgcgaag atacaccgcc
     1501 tgcacttccg tcccctgtcg gggccggcga agatactgaa cccgaggaac gtcttcaggt
     1561 tccaccgcca cgtgatgagc gtcgacggca aggtcgcgtt ccttggtggc tacaacatcg
     1621 ggaccctgta cgcggcggga tggcgcgaca cgcacgtcag ggtgcgcggc gatgttgcca
     1681 gggaggtgga caacgctttc gtcgacttct ggaacgccca ccgcacagag gagttgccca
     1741 ggataacccc caacaaggag cgtgactgga accccgccgt aagggtgcgc cgcaacgacc
     1801 catacatgag gatcttcccc ataagggaga tgtacatcga ggcgatagac cgcgccaact
     1861 cccacgtcta tctgacccac gcatacttcg tccccgaccg cgcgctgaaa gcgcacctga
     1921 tagacgcgac gaagcgtggc gtcgacgtac agctgctcgt ccccgagcag tcgaaccacg
     1981 tcaccgcgga ctggctcgcg cggcgtcact tctacgatct tctgaagaac ggtgtcagga
     2041 tcttcctcta caggcacatc atgatccact ccaagacggc caccgctgac ggtctctggt
     2101 cgacgatagg cagcgcgaac atcgaccgcc tcagcatgct cggcaactac gaactgaaca
     2161 tcgaggtcta cagcagcggg ctcgcagccc agatggagag gatgttcgag atggacaaga
     2221 caaacgccga agaggtgacc ctggaagaat gggaacgccg tccactccat gccaaggtcg
     2281 tcgagcgtac cctcaccagc ctgagcccgc tggtctagaa ggtatcaggt tttggtctcg
     2341 ggtctttgtc cgcgccgggt tggcagaggg ctaacgtccg ctttgtataa tgacgatact
     2401 cttgttttgg ggttattcgt gtaaggagga ctctttggcg cggtggcggc tccaggagcc
     2461 ggatgcggag atggtgtctg agcttgcgcg ggaggctgat gtggacatcc tgtgcgcccg
     2521 tgtgctggcc aaccgcggcg tcacgcctgg tgaggcggcg gacttcctct caccttcgct
     2581 cgccgccatc gggatgcccg atgaggacca gtggaacgtc gctgcagaaa gagttgcgaa
     2641 ggccatagcg gggggggaga ggatcgggat cttcggcgac tacgacaccg acggcatcac
     2701 ctcggccgcc gtcatgttcc tggctctctc acactacacg gaaaacgtca ccgtcaggct
     2761 gccgacccgc cagactggct acggtctcct ggaaccttac gtgcgcgacc tcttcgctga
     2821 gggcgtggat ctcctgatca ccgccgactg cggcatttcc aacaggagtg aggtggcgct
     2881 ggcgaacgag ctcgggatgg aggtggtcgt gacggaccat cacatcccgc cggaagatcc
     2941 gcccgatacg gcggtcgctg tgctcgaccc caagctgtgg aatcctgagg accccctggc
     3001 cggggtgggg gttgcctgga agttcgcctg ggccgtcgcc cgcaagctcg gcgacgccgg
     3061 gggcaggaaa cgtctggggc ggcttctaga tctcgtctcg gtcggcaccg tcgtcgacat
     3121 tgcgccgctc gtcggggaca acagggcgct cgctagtatg gggctcaagc acatgaacag
     3181 gggactcgct gccggagggg cgcgtcctgg catcgaggcc cttgtcaagg tggccggggt
     3241 gaggggggag atcgacgagg acgacctcgg gtggaagctg gggccgcgca tcaactccat
     3301 cgggagaata aagaaccctc ggcctgctct cgacctactt ctgacggatg atcgtcggga
     3361 agctttcagg atcgcttccc tcctgaacca gctcaacgcc gagcgtcagc ggcggactag
     3421 gtctgccgtc gagcgggcga tggcggaggt ggatcccgag caggacttca aggtcgtcgt
     3481 gacggaagag acgggaggga tcgcgggttt gatcgccggc cgcgtcgccg gcgctacggg
     3541 aaggccagcg gcgatactga cccgcagggc cgacgactcc tacggcggct cggcccgcgc
     3601 tggcgagacc gacgtcgacc tctacggcgc cctctacgcg gttcgtcacc tgatggggga
     3661 gtggggcggc caccgcaggg ccgccggcct ctccgtgaac cctggcaacc tcgacgcttt
     3721 cgtcgccggc gtcaacgagg ccgtcagggc ccagagcgaa cagaaaccgg agatcttcgt
     3781 gcccgccata gaggtcgacg cagagatacc gctagggagc gtctccaacg ggttactcga
     3841 ctggcacgag atgcttgccc ctttcggcag cggtaactac cgcccgatct tcgttacgga
     3901 ggacttgcgg atcgccggct cgcgcaagat ctgggagggc atgaacctcc tccacctcga
     3961 aggcggcatc aaggccaaga tggccggcga acccgacggg ttgccctcgg ggccattcga
     4021 cgctgcctac acggtcaccc gcagccgata ctccgggcag gccgagctcg agatagtgga
     4081 ctggaaggca ggccggtgaa cgccgtaggc gtcgacgtcg ggggcacgaa gatcgccgcc
     4141 ggggtcgtca cccccgaggg caagatcctg gacgaggtcc gctatcctac gccacactcg
     4201 cccgagacgc tggtcgagac catcgccagg gcgatctctg aggtcgggga cggtccccag
     4261 gtcggtggag tgtgcctcgc cgtgcctggc ttgatcctcg cccaggcgaa taaggtcgtc
     4321 ttctccccga acctgagggc ggtggaggga attccgctca aggacgagct ggagccgaag
     4381 atcgggatgc ccctcaccat tgagaacgac ggcaatgccg cagcgtgggg cgaattcagg
     4441 ttcggggtcg gcagcgaggc tgaccacctg gtcttcgtcg cgctcggaac agggatcggg
     4501 ggcggcatca tcacgcacgg cttcctgatg agaggtgcgc agggctcggg cggcgagctc
     4561 ggtcacgtaa ccatccatgc cacggggccc cgttgcgcct gcggcaacca cggctgcctg
     4621 gaagccctcg cctcgggcac cgcgatagga cgtcgcgccc gcgagcttgc ggtcgaaaat
     4681 ccagactcgg cgctcggacg cctggctacg aggcgccaaa tactcggcga ggatgtcaca
     4741 gagctcgccc gcgaagggga cgaggtggcg ctctcggtac ttgacgaggc agggaggtgg
     4801 ctcggcgttg gccttgctgg attcgtaaac atcttcaacc ccgaggtggt cgcggtcggc
     4861 ggaggcgtca tggaggccgg ggagctcata ctgagggcgg cccgcaggga agtcatcctc
     4921 cgcgccaggc ccccttcgcg cgacctggcg gacgtcaagg tcgctaccct cgggcccaga
     4981 tccggcgtcc tcggcgccgc agcgctcgcc agggacgcat cttccggaga gtacctgctt
     5041 ggcggctgag agatgcccct cacagtagtc ccaaccccca taggcaacct ggaggacatc
     5101 acccttcggg ccctca
//
LOCUS       DASTKM010000306         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_655331, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000306 DASTKM010000000
VERSION     DASTKM010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655331"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="VFQ10_12155"
     CDS             <1..1514
                     /locus_tag="VFQ10_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET9966140.1"
                     /translation="REAYPDLSVSISADIGQEYGEYERTLTTVVNTAVQPRTMLYMRN
                     FERSIDEKRFGGALSIVRSDGGAMSTEAVAERPIQIALSGPSGGVTGSAYLAREIGVP
                     DILTFDMGGTSTDVSLCLNGETPVRRDIQLGYFQFKSPAVDVHSVGAGGGSVAFVSAS
                     GALQVGPDSAGADPGPAAYGRGGEAPTVTDANVVLHRIPPGVALGGTLEMDEAAAREA
                     VGAITEQLGMGVEEAAEAIIDIVNENMHAALRVVSVERGHDPRNFGLVAFGGAGPMHA
                     NALGRLIHSRSVIVPPTPGVMSAFGFLSSDIQNEFPETYLRVAEETQAQDLRETIEGL
                     IAQADEWLEGEGVKEDKKQFDLYADCRYYLQNIQIPCRFELEELEDGSSAFLRGRFEE
                     AHRQRYNFDLPDSPLEIATVRVVGRGTIKGVSLLESEDGAGEDASGAITRKEQVYFDG
                     GWKETPIYDRTELRPGNAVAGPAVVVQDDTTTVIEPGFKGAVDSFGNILIEED"
     gene            1518..>2032
                     /locus_tag="VFQ10_12160"
     CDS             1518..>2032
                     /locus_tag="VFQ10_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET9966141.1"
                     /translation="MVLHEQAEPDFVREHDIDHVTLDIIENALKNIRHEMDRVLVTTA
                     VSPVIREQADEFPLIADRKGRMVVGQFGSPVDTVLENSPYTVEDLKDGDVIALNDPYM
                     MEGSTSHLPDILLVRPIFYEDDHIGYALQWGNLMDVGGSTAGSIPIDARSIYEEGVRL
                     PPVKLYDGGTL"
BASE COUNT          393 a          629 c          674 g          336 t
ORIGIN      
        1 tgcgcgaggc gtacccggat ctctccgtgt ctatctcggc ggacatcgga caggagtacg
       61 gcgagtacga gcggacgctg accacggtcg tcaacacggc cgtgcagccg cgcaccatgc
      121 tgtacatgag gaacttcgag aggtccatcg acgagaaacg cttcgggggt gcgctctcca
      181 tcgtccgctc cgacggaggg gcgatgagca ccgaggcggt ggcggagaga ccgatccaga
      241 tagccttgtc tgggccctcg ggcggcgtca ccggctccgc ttacctggca cgtgagatcg
      301 gagtccccga catcctgact ttcgacatgg gcggcacctc gaccgacgtc tcactatgcc
      361 tgaacggtga gacgcccgtg cggcgcgaca tccagctggg gtacttccag ttcaagtccc
      421 cggctgtgga tgtgcacagc gtgggggctg gcggtggttc cgtcgcgttc gtctcggcga
      481 gcggcgccct gcaggtcggg ccggacagcg ccggcgccga cccggggccg gcggcctacg
      541 ggcgtggcgg tgaggcaccg accgtaacag acgccaacgt tgttctgcac cgcataccgc
      601 ctggcgtcgc ccttgggggc acgctggaga tggacgaggc ggcggcgaga gaggctgtgg
      661 gggccatcac agagcagctc gggatgggcg tcgaggaggc ggccgaggcg atcatcgata
      721 tcgtcaacga gaatatgcac gccgccctgc gcgtggtcag cgtcgagcgc ggacacgacc
      781 cgaggaactt cgggctggtc gccttcggcg gggctgggcc gatgcacgcc aacgcgcttg
      841 ggcgcctgat ccactcccgc tccgttatag ttccccccac gccaggcgtg atgagcgcct
      901 tcggattcct ctcatcagat atccagaacg agttcccgga aacgtacctg cgcgtcgcag
      961 aggagaccca ggcgcaggac ctgagggaga cgatcgaagg gctcatcgca caggccgacg
     1021 agtggctgga aggcgaggga gtgaaggagg acaagaagca gttcgacctg tacgcggact
     1081 gtcgctacta cctacagaat atccagatcc cctgccgctt cgagctcgag gaactcgagg
     1141 atgggagcag cgcctttctg cgcgggcggt tcgaggaggc ccatcgccag cgctacaact
     1201 tcgacctgcc tgactctccg cttgagatag ccacggtccg cgtcgtcgga cgcggcacca
     1261 taaagggcgt cagcctcctg gagagtgagg acggggctgg ggaggatgcc tctggtgcta
     1321 tcacgcgcaa agagcaggtg tacttcgacg gcgggtggaa ggagaccccg atctacgacc
     1381 gtaccgagtt gcgccccgga aatgctgtag ctggtcccgc cgtcgttgtg caggacgata
     1441 cgactacggt catagagccc ggtttcaaag gagctgttga cagcttcggg aacatcctga
     1501 tcgaggagga ttaggtaatg gtgttgcacg agcaggccga gccggatttc gtccgggagc
     1561 atgacataga ccacgtgacc ctcgacataa tcgagaacgc cctcaagaac atccgccacg
     1621 agatggacag ggtgctcgtc accaccgccg taagccccgt aatccgggag caggccgacg
     1681 aattccccct catagcggac cgtaagggcc gcatggtcgt gggccagttc gggagccccg
     1741 tagacaccgt gctggagaac tccccctaca ccgtcgagga cctgaaggac ggcgacgtca
     1801 ttgccctcaa cgacccctac atgatggagg ggagcacctc ccacctccca gacattctat
     1861 tggtgcgtcc catcttctac gaggatgatc acataggtta cgccttacag tggggcaacc
     1921 tcatggacgt tggcggctcg accgcggggt ccatccccat cgacgcgcgc tccatctacg
     1981 aggagggcgt caggctaccg ccggtcaaac tctacgacgg cggcactctg aa
//
LOCUS       DASTKM010000307         5510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_477069, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000307 DASTKM010000000
VERSION     DASTKM010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477069"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFQ10_12165"
     CDS             complement(<1..724)
                     /locus_tag="VFQ10_12165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9966142.1"
                     /translation="MVGALAVVAIALPSIGEELGFSRSGLQWVISAYVLAFGGFLLPA
                     GRAADLWGRRRVFVFGLALFSVASLVCGLSGSAALLIAARAAQGFGAALVTPAALSIL
                     TGVYPEGEGRSLAVGFWTAAQAGGGASGWIVGGFLAQGPGWEWVFFATVPVGALGVLL
                     APFLLHERSDPSASPKLDAAGAAIVTAALALLVYSCTQAEEAGLASPGTLGSLGLSVA
                     LVAAFVGVESRVRHPLVPLGVFR"
     gene            862..1485
                     /locus_tag="VFQ10_12170"
     CDS             862..1485
                     /locus_tag="VFQ10_12170"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HET9966143.1"
                     /translation="METPDHILALRLVGGNLALDFANTAEGTPEGEIEREHLFGYQDL
                     AFWGHRVGLLSGEDVVRLLQKCHERPVEADAVFARALEFRGHLYELFRAVAEGHDPPE
                     EDVEALRRFECEALSRAKLVPSGDGFAWQWAAGDDLSGVLWPVAHAATELLTSGPLGR
                     VKNCAGCNWLFVDESKNRSRRWCSMEECGTQAKMRRYVARRAAKRKS"
     gene            complement(1602..2288)
                     /locus_tag="VFQ10_12175"
     CDS             complement(1602..2288)
                     /locus_tag="VFQ10_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9966144.1"
                     /translation="MGSDEGTRPGTNQPPAVRPSAPRINWQSALYVFVLAFACVLLVP
                     LTAFWWIVPVLGAAVPISLAFLARPDLGFLPADNQERELVDALVDRGELTPATAAMRT
                     SLTVDEASRMLEDLARKGRLRPRAGDGLVSYALRDRDPSETSPIGLSGAPDAVAGGTP
                     RALSEPLSERELEVLALLASGRTNAEIARDLYVALGTVKSHSGNIYRKLEASNRTEAV
                     SRAREMNLLR"
     regulatory      2627..2713
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            2735..3742
                     /locus_tag="VFQ10_12180"
     CDS             2735..3742
                     /locus_tag="VFQ10_12180"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9966145.1"
                     /translation="MRRIVLMVTMAVLVALSLLVSAGFASAKPNGHVVRPGESIQKAI
                     KAADPGDTIVVLGGVHHGTVVIKKDGISLRGVDAVLKPPAKPSSPCGASGFCVTADNV
                     SISGFTVRNFPATGIIAMGARNPKFMTNRAFNNGEYGIATFSSPGTKLIANLTRGSDD
                     AGLYVGDSPHANATIAANETYDNFLGILVRNARHGKIAGNQVHNNCVGMFILADAPGP
                     AGVFDVRGNKVVNNTRACPAFEGAPPLSGVGIALLGARGMEINSNHIIHNVPSGPTGF
                     SGGVVVVSGFEGTPPTNNTVTGNTILRNKPDIVWDKSGSGNRLKPNDCKTSKPARLCK
                     G"
     gene            complement(4233..4442)
                     /locus_tag="VFQ10_12185"
     CDS             complement(4233..4442)
                     /locus_tag="VFQ10_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966146.1"
                     /translation="MMVEVWENGLVVLLVFATFVLLPLVVAFFAGDAVAGALPHATWR
                     AASPISPGRAGRREESGAACGYRPR"
     gene            complement(4439..4630)
                     /locus_tag="VFQ10_12190"
     CDS             complement(4439..4630)
                     /locus_tag="VFQ10_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966147.1"
                     /translation="MSTETLVLVGGASPAWTIDIGRQVADALPNGQHRVLEGQEHVVP
                     PEVLVPVLAEFFTGRNHPD"
     gene            complement(4627..5154)
                     /locus_tag="VFQ10_12195"
     CDS             complement(4627..5154)
                     /locus_tag="VFQ10_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9966148.1"
                     /translation="MCDRALTGSTAEELAKHFTVLNYDRRGRGDSGDTGPYAVEREIE
                     DIGALIVEAGGTASVYGHSSGAGLVLHATAYGVPTDKLVLHEPPYNTDGEEEQRISRE
                     YALDLKALLSEGRRGDAVELFFTTVGMPREMVGGMRQTPGGRSWRRWRPRSPTIRRSW
                     ATAPAGGCRPSLPAV"
BASE COUNT         1050 a         1805 c         1741 g          914 t
ORIGIN      
        1 agcggaaaac gcccaaaggc acgagcgggt gacggaccct ggactcgact cccacgaacg
       61 ccgccacgag cgccaccgac agccccaaag aacccagggt gcccggggag gcaaggcccg
      121 cctcctccgc ctgcgtgcaa ctgtagacca ggagcgccag ggccgccgtg acgatcgccg
      181 cgccggccgc gtcgagcttc ggggaggctg acgggtcgct cctctcatgt agcaaaaacg
      241 gggcgagcag cacgcctaaa gcccccacgg gcaccgtggc gaagaaaacc cactcccacc
      301 ctggtccctg cgccagaaag ccccccacga tccaacccga ggcgcccccg cccgcctggg
      361 ccgccgtcca gaagcctacg gccaggctgc gtccctcgcc ctcggggtat acgccggtca
      421 ggatggaaag cgcggccggc gtgacgagcg ccgcaccgaa tccctgcgcc gcgcgggccg
      481 cgatgagcag cgccgccgag cccgagaggc cgcacacgag cgaggccacc gagaacaggg
      541 cgagcccgaa tacgaacacc cggcgcctgc cccagagatc tgccgccctc cctgcaggca
      601 gcagaaagcc gccgaaggcc agcacgtagg cggagatcac ccactgaagc ccggaccgcg
      661 aaaacccgag ctcctccccg atggagggca aggcaatggc caccaccgcg agggcgccaa
      721 ccaccacaaa ctccgccgca cacaggagcg cgagagccgg ccaccgccgc ccgctcttcc
      781 ctttgtttcc accgtaacct tgcacgaggg taacacaata cccgattgcg gcgtctgcgt
      841 aaaccggtaa aatgaatcta gatggaaaca ccggaccaca tcttggcgct tcgcctcgtc
      901 ggggggaatc tcgccctgga cttcgcgaac acggctgagg gaacgccgga gggtgagatc
      961 gagcgggagc acctcttcgg gtaccaggat ctggcgttct gggggcaccg cgtcgggctc
     1021 ctttccggag aagacgtcgt acggctgctg cagaagtgtc atgagagacc tgtggaggcc
     1081 gacgcggtct tcgcccgcgc cctcgaattc cgggggcacc tgtacgaact atttcgggct
     1141 gtggccgaag ggcacgaccc gccggaggag gatgtcgagg cgctgcgccg gttcgagtgc
     1201 gaggccctct cccgcgcgaa gctcgttcct agcggcgacg ggttcgcctg gcagtgggcg
     1261 gccggggacg acctgtccgg ggtgctctgg ccggttgcgc acgcggcgac cgagctgttg
     1321 acctcagggc ctttggggcg ggtcaagaac tgcgccgggt gcaactggct cttcgtcgac
     1381 gagagcaaga acagaagccg ccgatggtgc tcgatggaag agtgcggcac ccaagcgaag
     1441 atgcggcgct acgtcgcgcg ccgggcggcc aaacgcaaga gctgagattc ggaagcgtca
     1501 aacagggttt tcgcgaggag ccttcgtatc ccaaaacgaa ggttggcagg atctcgtaca
     1561 ggcgcacggg ctcgcgtccg aagcgggttc ctagcgggcg ctcagcgcag caggttcatc
     1621 tcgcgggccc ggctgacggc ctcggtgcgg ttggacgcct cgagcttgcg gtagatgttg
     1681 ccagagtggc tcttgacggt gcccaaggcg acgtagagat ccctggcgat ctctgcgttg
     1741 gtcctgccgg acgccaagag ggccaatacc tccagctctc gctcgctcaa tggctcgctg
     1801 agggcccggg gtgtgccgcc cgccaccgcg tccggggctc cggagaggcc tatcggcgat
     1861 gtctcggatg ggtccctgtc gcgaagagca taggatacga gaccgtcgcc cgctcgcggt
     1921 ctcaggcggc ccttgcgagc aagatcctcg agcatcctcg aggcttcgtc gactgtgagc
     1981 gaggtgcgca tcgcagccgt ggccggggtg agttctcccc ggtccacgag cgcgtcgacc
     2041 agctcacgtt cctggttgtc tgcgggcagg aatccgaggt cgggcctggc gaggaaggcc
     2101 agtgagatcg gcactgcggc gccgaggacc ggaactatcc accaaaaggc cgtgaggggg
     2161 accaagagca cgcaagcgaa ggcaaggacg aagacgtaca gggccgactg ccaattgatc
     2221 ctcggcgccg agggtcgcac tgcgggaggt tggttcgtcc ccggtctcgt gccttcgtcg
     2281 cttcccatgg ccaagggatc atcggatcct gacgcgcggc ggccaggacc ttccttccct
     2341 acgccacttc gatcttcgtt accccagggc tccatgcgcg tcacctcgtt gtacttcgcc
     2401 ggaccgcccg cgtccgaggc ttggccttcg ccgatgctcg ggcgaggcag tgtccctaca
     2461 atcgagtgta cgcgctcccg cggtcgattg tttcacgaac cacacgaagg tgtgcctaca
     2521 gagagaggcg ggtgtaactc gactccagtg ctcgcaacaa caagcaagaa taggccgtgt
     2581 aaggcatata ctggaacaca caagtttggt actgtgttgg cttgtatacg cgtgcagagg
     2641 cttggaacca cgtttcgtga aggtgggcac ttaggagacg tggggagcca gtagtgcaac
     2701 cggctgccgc actacatata aggaggttgc gcttatgagg cgcatcgtct tgatggtcac
     2761 gatggcggta ttggtggcct tgtcgctgct tgtgtctgca ggcttcgcct ctgcgaagcc
     2821 caacggacac gtcgtccgtc cgggcgaatc gatccagaag gcgatcaaag ccgccgatcc
     2881 gggcgacacc atcgtggttc tcgggggcgt ccaccatggg accgtagtga tcaagaagga
     2941 cgggatctcg ctgcgcggcg tcgacgctgt gctcaagccg ccggcaaagc cctcctcccc
     3001 ctgcggggca tcaggctttt gcgtcacggc agacaacgtg tccataagcg gcttcaccgt
     3061 gcggaacttc ccggcaaccg ggatcatcgc gatgggagca cgcaacccca agtttatgac
     3121 taatcgtgcc ttcaacaacg gggaatacgg gatcgccacc ttctcctcgc ccggaacgaa
     3181 gttgatcgcc aaccttacac gcggatccga tgacgccgga ctctacgtgg gagattcgcc
     3241 gcacgccaac gcaaccatag cggccaacga aacctacgat aatttcttgg gcatcttggt
     3301 gcgcaatgcc cgccatggca agatcgccgg taaccaggtc cacaacaact gcgtggggat
     3361 gttcatcctc gccgatgcac cgggtcctgc gggtgtgttt gacgtgcgtg gcaacaaggt
     3421 tgtgaacaac acgcgcgcgt gcccggcctt tgaaggtgcc ccgccgctct ccggagtcgg
     3481 cattgcgctg ctcggggcca ggggcatgga gatcaatagt aaccacatca tccataacgt
     3541 tccatcagga cccaccggct tctcgggcgg tgtggtggta gtcagcggct tcgaagggac
     3601 gccgcccacc aataacaccg tcaccgggaa cacgatcctc cgaaacaagc ccgacatcgt
     3661 ctgggacaag agcggttctg gcaatcgtct caagcccaat gactgcaaga cgagcaagcc
     3721 ggcacgcttg tgcaaagggt gaagtaggtc tatcaagggc gaaggttacg ccactttggg
     3781 ggccgaggct gtttgcctcg gccccacttc ttgccctaat tcccccgaat gtgtggagaa
     3841 acagtccgaa aagcctcata tgcgagacga tctcctcgca atcgatggct cgacccggca
     3901 tcctatcgtc ctgttctccc ccgaccggct cgcggccagg gagtagctat ggagatcctc
     3961 gatgcgcgag agaccgaagc ccgcctgccc tacccggagc tggcggactc catcagggaa
     4021 gttatggatg gaatgcggga ggggaagctg gggacctgat ccaggccgag cacgcaggag
     4081 cattcggttg ggaggatgcc actcagctgg aggatgccct cctctggtcc aacaagccag
     4141 gaggcaccat cgtcttcaag agtgtcggcc acgccctgtg ggatcttgcc gccgcccgta
     4201 cggccttcgg gttttgaaca agcggatcac actcagcgcg gtcgataacc acaagccgcg
     4261 cctgactctt ctcggcgtcc cgcccggccc ggcgagatgg gcgaagccgc ccgccaggta
     4321 gcgtgcggca aggcgccggc caccgcgtcc cctgcgaaga acgctacgac gagcggcaac
     4381 aggacgaagg tggcgaagac gagcaggacc acgagcccgt tctcccaaac ctccaccatc
     4441 aatcggggtg gtttcgtccg gtgaagaatt ctgccagcac cggcacgagc acctcgggag
     4501 gcacgacgtg ctcctggcct tccaggacac ggtgctgccc gttcggcagg gcgtccgcaa
     4561 cctgcctgcc gatgtcaatc gtccacgcgg ggctcgcccc gccaacgagg acaagggtct
     4621 cggtactcac acggccggta agctcggccg gcaccctccc gccggcgctg ttgcccatga
     4681 tctccgaatc gtaggcgagc gtgggcgcca tcgcctccag ctccgcccac ctggggtttg
     4741 gcgcatccct ccgaccatct cccgcggcat ccccaccgtt gtaaagaaca gctcgaccgc
     4801 gtcgccacgg cgaccctcag acaacaatgc cttgaggtcc aatgcgtact cccgcgagat
     4861 ccgctgctcc tcctcgccgt cggtgttgta gggcggctcg tgcaacacga gcttgtcggt
     4921 gggtacgcca taggccgttg cgtggaggac gaggcccgcg ccggaggaat ggccgtacac
     4981 cgacgccgtc ccaccagcct cgactatgag agcgccaatg tcttcgatct cgcgctcgac
     5041 cgcgtacggc ccggtgtcgc cgctgtcgcc ccggccacga cggtcgtaat tcaacacggt
     5101 gaaatgcttc gccagctcct ctgcagtcga accggtcaat gcacggtcgc acatcgcgcc
     5161 ccccaccacg atgacaggcg gaccatcgcc cagccggtcg aaggcgatct aggtgccgtc
     5221 gctcgaagta accttgtcca tgaactcgct tctttcctcg tccgtcgggt caccatagtc
     5281 cggttccagc atacggatat aggcctaagg cacaaccagt acgctcataa attagactga
     5341 ggccttcctg ccgggttagc gtcccctgtg ggtcgctggg tcttgtggct gtgagattac
     5401 gtccacggcg gttgctagcc caggacttca tgggagtccg gtagtactct tacgttcggc
     5461 cgggggaacg agactgatga cacgcagggc cgtcatcctc tccaggccct
//
LOCUS       DASTKM010000308         8165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1009575, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000308 DASTKM010000000
VERSION     DASTKM010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009575"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFQ10_12200"
     CDS             <1..211
                     /locus_tag="VFQ10_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9966149.1"
                     /translation="NIAPNSPAERAGVKPGDVIIGLKGEPVHSNDDLLNLLDASSIGQ
                     DATLRVLRGNREVSLTVRPREQPAE"
     gene            482..718
                     /locus_tag="VFQ10_12205"
     CDS             482..718
                     /locus_tag="VFQ10_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HET9966150.1"
                     /translation="MPDVTFVVNDVTDAEESEKLERALTRLEFVDLVNVAFEKGLVAV
                     SYEGGEEELGKIESAIEEAGHNAEPSPGADPDVD"
     gene            722..1147
                     /locus_tag="VFQ10_12210"
     CDS             722..1147
                     /locus_tag="VFQ10_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966151.1"
                     /translation="MPEPSVSVSEAALFLDVPQEEARKLADAEGNVPLETLRSRAGAA
                     TIARELASAREEVGRLSSQLREVRASNVRLQKELRGSVEERRRLTEEVLELRAAAEER
                     LMLMERIEHIARVEQELEESSSELERLRNRRLLGRLLNR"
     gene            complement(1157..1861)
                     /locus_tag="VFQ10_12215"
     CDS             complement(1157..1861)
                     /locus_tag="VFQ10_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565941.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HET9966152.1"
                     /translation="MEFMTGEWFGRLVGVLVVNLILSGDNAVVIAMAARTLEGANRRK
                     AIIWGAAGAVVLRLIFAAVITLLLEVPLLQVVGGLLLVWIAWKLIHEGDGEGDDEEKV
                     KAGSSTWQAVRIIIVADAVMSLDNVIALVQAARIGGEVDISLLVIGLATTIPLVIFGA
                     ALLTSLLDRFPFLVYLGAGLLIYLAVEMLLADKVTHPYLKHYHSIEWIIGITAAGIFI
                     LVAWLWARRSSSEEAG"
     gene            complement(1946..2965)
                     /locus_tag="VFQ10_12220"
     CDS             complement(1946..2965)
                     /locus_tag="VFQ10_12220"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966153.1"
                     /translation="MTIIELVLSLAAILGAAILFTNAVEIMGDRLNLGAGAVGSVLAA
                     VGTALPETMIPLVAIFGALLFGSGGAAAGEIGIGAILGAPFLLATLALFIVGAAALGF
                     KGRRETGSEITADRQTAIPDLVFFLLCFVPAAAAGILPLPLPLKIGLAVALLVAYVLY
                     VVRTVKKGGETEGDVPARLTLWPFRSQGPTWAAAGQLIGTIAVMAFGAHLFVGAVEHL
                     SKSVGIPAGLIALVLAPLATELPEKFNSVIWLRDNKDTLAIGNITGAMVFQSTIPVSV
                     GVLFTPWRLDFISAVAAIFAILSGIVFLGFLLRKAPLRAFHMLGAGSLYVVFVVAAII
                     DLAGG"
     gene            3048..3827
                     /gene="larB"
                     /locus_tag="VFQ10_12225"
     CDS             3048..3827
                     /gene="larB"
                     /locus_tag="VFQ10_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET9966154.1"
                     /translation="MDELEKTLTAVASGELSVESAAGRLKRGKVRYLDEFAALDLGRS
                     VRKGVPEVVYARSKSPAQVASICATLLESCERVIVSTPTPEHEAKIRATLPETQLRWA
                     GRSLIIGGGEPAPSGGRVGALSAGTSDLPVLEEALAVAGEMGASVMGFHDVGVAGVHR
                     LAGPLEELRAFDPDCLVVAAGMEGALPSVVSGLVAVPVIGLPTSTGYGLGGDGTAAIL
                     GMLQTCSPGLSVVNVDNGVGAGVTAALIANRAAAGRKSSPD"
     gene            3835..4371
                     /gene="thpR"
                     /locus_tag="VFQ10_12230"
     CDS             3835..4371
                     /gene="thpR"
                     /locus_tag="VFQ10_12230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET9966155.1"
                     /translation="MRTFVAVFPPQRVREALFRAARDVPASKAFRLISPEKLHITLKF
                     LGNVAEDDLSRVEQALEQLRGRHEPFEVETSGFGAFPSERKARMLWAGVGEGSGPLRA
                     VAQSVDDLLEPAGFGREQRSYVPHLTLGRARGRQAKLEGGSVSLPALRFKVSGVDLVE
                     SVSGEGGVSYSVLETYSF"
     gene            complement(4543..5082)
                     /locus_tag="VFQ10_12235"
     CDS             complement(4543..5082)
                     /locus_tag="VFQ10_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966156.1"
                     /translation="MADQIEDCLHSEGLRATSGSEYCCPLCEECLDLDGVSRWVHEAE
                     RSRDEAYSMVEGDGFGEIVRQELQREVYRRRRVMYELENVPRVVTARPEMILVIHDAV
                     DASYECRIFYKEPRPAGVVEQVSVGAVLDRILELHSNPDPVVRLISEKIEEFHVFRSK
                     LSEDGEPAPERRVFYANEL"
     gene            5418..6302
                     /locus_tag="VFQ10_12240"
     CDS             5418..6302
                     /locus_tag="VFQ10_12240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9966157.1"
                     /translation="MFSFGGEAHLVLNPSAGKGRAGGLRASVSRFFEEQGVLPVWHVT
                     QGPGHAGSIVRGLPEEVSVVAVGGDGTIHEVAAACAGTGRIMGVLPAGSGNDYVKALG
                     VGTGLRRALEVLVGGNVRVVDTAEVDGIPFNNGLGIGFDAEVAAGAARAPAYLGGTGR
                     YLWSVGRLLKDFRCHEARLVIDSEVVEAKTILVAVALGTTYGSLFRLAPGAVLDDGLF
                     DVIWSEEVSRAEVLRLIPAALRGTLSKRRKVHTARAREVEIELSEEVPAHVDGEMLAR
                     TRHFKAQVLPGTLRILTP"
     gene            6491..7330
                     /locus_tag="VFQ10_12245"
     CDS             6491..7330
                     /locus_tag="VFQ10_12245"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET9966158.1"
                     /translation="MFPGQGGGVAEPEPEMVPEIQRVVGERIPEQVKIFVRAARDADE
                     SRTMNVRPDVVAGHSLGEYGAAYAAGCFDFDTGLWLVAQRDHFLSEAARECPGGMVAI
                     LKTDPAEVEKALGAPGGPVVANYNSPRQTVVSGLRDALGDAVSKIRGRKIPLNVSFAA
                     HSPYVAAAGEKMRGVLDSVEFHAPQVPIVSAMDGAVLTKADAVKGALKDQMVAPVRWV
                     RVVETLAKMRVEEWIELGGSGVLTRMLKDFELPSLNGITFEDLRSRLYGQAQNLLPRK
                     EGE"
     gene            complement(7409..>8165)
                     /locus_tag="VFQ10_12250"
     CDS             complement(7409..>8165)
                     /locus_tag="VFQ10_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HET9966159.1"
                     /translation="DKYSMGEEKTLIKNIFNEDKGKWVPRRVELPDGNKRPGLPLDAG
                     HIVNNTETLLNIYNAFFLGKPVTTKYLQVFGPELELELFEAPLGSSATELLKLSGVDA
                     EAEAGNLSVIDGGPYLNEMGIESLGESDAYVRRTTNGFLVIPKGVASKEYANIKTRRP
                     EGVTSLVGKVSAVSIPLGGRFLKPATALVSEGDEVSFGQKIGEPVDEGFSIGVWSSMD
                     GPISSIEEDIVQISGGAVTKEETRAETEAEATR"
BASE COUNT         1607 a         2536 c         2585 g         1437 t
ORIGIN      
        1 gaacatagcc ccgaacagcc cggccgagag ggccggcgtt aaaccgggag acgtcatcat
       61 cggcctgaaa ggggagccag tccactcgaa cgacgatctc ctcaacctgc tcgacgcatc
      121 gtccatcggc caggacgcca cactacgcgt gctgcgaggc aacagggagg tctcactcac
      181 cgtgcggccc cgagaacagc cggcggaata gctggtcagc acttcagcac gctgcccggc
      241 ttcgccaggc ggcttgtcag catttcagct agtcagctag aacatggtcg gcgatcggcg
      301 gagatgacaa ctagaccaag atggaccaaa cttacactcc ccacagagcg gcgtagccgt
      361 gtggtggtct cgccgcttgg acagaaagct gaaatgctga caagcgacag ccggaggctg
      421 gagcgtgctg acaagccgcg aagcggcgtg atgaaatgct aagcgaccga agggagcgac
      481 aatgcccgac gtgactttcg tggttaacga cgttacggac gctgaggagt ccgagaagct
      541 ggagcgggca cttacgcgcc tggagttcgt ggacctggtg aacgtggcct ttgagaaggg
      601 gctcgtcgcc gtctcctacg agggtgggga ggaggaactc gggaagatcg agagcgccat
      661 cgaggaggca ggccacaacg ccgaaccttc ccccggcgcc gaccccgacg tggactaatc
      721 catgcccgaa ccctccgtct ctgtatcaga agcggccctt tttctggatg ttccgcaaga
      781 ggaagcgaga aagctagccg acgccgaggg caacgtccca ctggagactt tgaggagccg
      841 ggccggagca gcgaccatcg cgagggagct ggccagcgcc cgcgaggagg tcggaaggtt
      901 gtcaagccag ctaagggagg ttcgcgcttc caatgtacgt ctccagaaag agctgagagg
      961 ctcggtcgag gagcgcaggc gcctgacaga agaggttctg gagctgaggg cggcggccga
     1021 ggagaggctt atgctcatgg agcgcatcga gcacatagcc cgtgtcgagc aggaactgga
     1081 ggagagttcc tccgagctgg agcgcctcag gaacaggaga ctcctgggta ggctcctgaa
     1141 ccgctagagc ggttccctac cctgcctcct cgctgctcga tcttcgtgcc caaagccagg
     1201 ccacgagtat aaagatgccg gccgcggtga tgccgatgat ccactcgatg ctatggtaat
     1261 gcttcagata agggtgggtt actttgtcgg cgaggagcat ctccacggcc aggtagatga
     1321 gcagcccggc gcccaggtag acgagaaagg ggaaacggtc aagtagagac gtcagcaacg
     1381 cggcaccgaa gatcaccagg ggtatcgtcg tcgccagccc gatgacgagc aacgagatgt
     1441 cgacctcgcc cccgatcctg gcggcctgga ccagtgcgat cacgttgtcc agcgacatca
     1501 ccgcgtcagc cacgattatg atcctgaccg cctgccaggt gctcgaaccg gcctttacct
     1561 tctcctcgtc gtcgccttca ccgtcaccct cgtggatcag cttccaggcg atccagacga
     1621 gcagcaatcc tcccaccacc tgcagtaaag gcacctcaag cagcaaggtg ataacggcgg
     1681 cgaagattag cctgagaacg acggcccccg cggcgcccca gatgattgcc ttcctcctgt
     1741 tggccccttc gagggtgcgc gccgccatcg cgatgaccac agcgttgtcg ccggagagga
     1801 tcaggttcac gaccagtaca ccaacgagcc ggccaaacca ctctcccgtc atgaattcca
     1861 tctgccccgg cttcctcctc gaccgtacta caggttgcct atcttatcag gaaaggggga
     1921 aaataagtgc gaggcccggt ccaactcagc cgccggcgag gtcgatgatc gctgccacga
     1981 cgaagactac gtagagtgat ccggcgccga gcatatggaa cgcccggagc ggggccttgc
     2041 gcagcaggaa accgaggaag acgatgcccg agaggatcgc gaagatggcc gcgaccgcgc
     2101 taataaagtc gagtcgccac ggcgtaaaga gcaccccgac cgagaccggg atggtgctct
     2161 ggaagaccat cgcccctgta atattgccga tggccagggt gtctttatta tccctgagcc
     2221 agatgacaga gttgaacttc tcgggcagct ccgtcgccag aggcgcgagg accagcgcga
     2281 tcaggccggc gggaattcct acggacttgg agaggtgctc cacagcaccg acgaagaggt
     2341 gggctccgaa ggccatcacg gcgatcgtcc cgatcagctg cccggctgcg gcccaggtcg
     2401 gcccctgaga gcgaaagggc cagagcgtga gcctggcggg aacatccccc tcggtctctc
     2461 cgcccttctt taccgtacgt accacgtaga ggacgtaagc gaccagcagg gccacagcaa
     2521 ggccgatctt cagcggcaga ggcaggggca ggatgcccgc agcggcggcc ggaacaaagc
     2581 acaacaggaa gaacacgagg tcggggatgg cggtctgcct atcggctgtg atctcactcc
     2641 ccgtctccct tcggcccttg aagcccaggg cagccgcccc gacgatgaac agggctagcg
     2701 tcgcgagcag gaaaggcgcg ccaaggatcg cgccgatacc tatctcgcct gctgcagcac
     2761 cgcccgagcc aaagagtaag gcaccgaaga tagcgaccaa tgggatcatg gtctcaggca
     2821 atgccgtgcc gacggccgcg agcacgctcc ctacggcccc tgctccgagg ttcagccggt
     2881 cgcccatgat ctctacagcg ttggtaaaaa gaatcgcggc gccgagtatc gccgccagag
     2941 ataggaccaa ctcgattatc gtcaaggact ctccttatga ccactagagg taggaacgtg
     3001 ctagattctt acccgctggc aagcttacta cacccggaag cgggggcatg gacgaactcg
     3061 agaaaaccct gacagcggtc gccagcggcg aactctctgt agagtcggcc gccggacgcc
     3121 tgaagagggg caaggtccgc tacctcgacg agttcgccgc cctcgacctc ggacgctccg
     3181 tgaggaaggg cgtgcctgag gtcgtctacg cccgcagcaa gagccccgcc caggtggcaa
     3241 gcatctgcgc gactctgcta gagtcgtgcg agcgtgtgat cgtaagcacc cccacccccg
     3301 agcacgaagc caaaatccgc gctacgctcc ctgagaccca gctccggtgg gccggacgct
     3361 ccctgattat cggtggcggc gagccagcac cgagcggggg tcgcgtgggt gccctgagcg
     3421 cgggcacctc ggacctgccc gtcctcgagg aggcgctcgc ggttgccggc gagatgggtg
     3481 catccgtgat gggcttccac gacgtcgggg tagccggcgt ccatcgcctc gccggcccgt
     3541 tggaggagct gagagcgttc gatcccgatt gcctggtggt cgccgccggg atggaaggcg
     3601 ccctgccgag cgtggtctcc ggcctcgtcg ccgtgcccgt gataggactc ccgacctcca
     3661 ccgggtacgg cctcggcggg gacgggaccg cagccatcct cggcatgctc cagacctgct
     3721 cccccggtct ctctgtcgtc aacgtggata acggcgtcgg cgcaggggtc accgccgccc
     3781 tgatcgccaa ccgcgccgcc gcaggccgca agtccagccc cgactaggac cgcggtgcga
     3841 accttcgtcg ccgtcttccc accgcaaaga gtcagggaag ctctgtttcg cgcggcacgc
     3901 gacgtgccgg cgagcaaggc tttccgtctc ataagccctg agaagctcca cattaccttg
     3961 aagttcctgg gcaatgtggc cgaagacgac ctgagccggg tggagcaagc actcgagcaa
     4021 ctacgcggga ggcacgaacc gttcgaggtc gagacctcgg gcttcggggc cttcccctcc
     4081 gagaggaagg cccgtatgct ctgggccggc gtcggagaag ggtcagggcc gctgcgcgcg
     4141 gtggcccaga gcgttgacga tctcctcgag cccgccggat tcgggcgaga gcaacggtcc
     4201 tacgtgcccc acctgaccct cggcagggca cggggccgcc aggccaagct cgaaggcgga
     4261 agcgtatccc tgccggcgct ccgctttaaa gtatccggcg tggatctggt ggagagcgtg
     4321 tcaggagagg gcggtgtcag ctactccgtc ctcgagacct actccttcta gaaggcggta
     4381 accagaggcc cgatggacat tatgatgaag agcaggatca ggatcattac gaccagggca
     4441 ataaaggtac tcccgctcat gcgaccgagc ctcttagata ggtatccatc tggcggtcaa
     4501 ttctagccgt tgcttgacgc ctacgccagc gcctcagggg tttcagagct cgttcgcgta
     4561 gaataccctg cgctctggcg caggctcccc atcttcggag agcttcgagc ggaagacgtg
     4621 aaactcctcg atcttctcgg agatcagcct tacgacagga tcggggttgg agtgaagctc
     4681 caggattctg tcgagtaccg ccccaacgct tacctgctcc acaactcccg ccggtcgcgg
     4741 ctccttgtag aagatgcggc actcataaga agcatcgaca gcatcgtgaa ttacaaggat
     4801 catctccggc ctcgccgtca ctacccgggg cacgttctct aactcataca ttaccctccg
     4861 tcgtcggtag acttcccgct gtaattcctg gcgtactatc tcgccgaacc cgtcaccttc
     4921 gaccatagag taagcctcat cccggctcct ctcggcctca tgaacccacc ggctaacgcc
     4981 gtcgagatcc aggcactcct cacataaagg acagcagtat tcggatccag aagtagccct
     5041 caagccctcc gagtgcaggc aatcttcgat ctggtcagcc atatggaata gtattattac
     5101 tcctttccca accttgcgcg actctcacat agagcacgaa gtccctctgt acctggactt
     5161 taactctatg ttaaatggaa gtctatagtg gcagggacct tcgaatggcc ctcggtaagt
     5221 cttcggaaga gaccaggtcc ctattctacc tgcatagaag tctctgtacg gtcgtcccgt
     5281 ctctgcgaac tccgcccctg gaccgttact tcgacctggc tagtataggc cagctttcgt
     5341 cgcaacctac acctcgagtc gagcttgaac gaccttccgg ccgggccgct ggactgcggt
     5401 tctggtattt tccggctatg ttctccttcg gaggagaggc acatctggtc ctcaatccgt
     5461 cggccggcaa gggacgtgcg ggagggctac gggcgtccgt ctcgcggttc ttcgaggagc
     5521 aaggggtgct gcctgtctgg cacgttacgc aggggcctgg ccacgcgggc agcatcgtgc
     5581 gagggctgcc cgaggaggta tctgtagtgg cggtcggtgg agacgggacg attcacgagg
     5641 tggcggcagc gtgcgccgga acgggccgga tcatgggcgt gctgcccgct ggtagcggca
     5701 acgactacgt caaggctctg ggggtaggca cggggctcag gcgggcgctg gaggtactcg
     5761 tcggagggaa cgttcgagta gtggatacgg cagaggtcga cgggatccca ttcaacaacg
     5821 gcctcggtat cggcttcgat gccgaggtgg ctgccggagc cgcccgggcg cctgcgtatc
     5881 tcggggggac aggacggtac ctgtggtcgg tcgggcgatt actcaaggat tttcggtgcc
     5941 atgaggccag gctcgtcatc gacagcgagg tcgtcgaggc caagaccata ctcgtcgctg
     6001 tggcccttgg caccacgtac ggctcgctgt tccggctcgc gcctggggcg gtactggacg
     6061 acggcctatt cgatgtgatc tggtccgagg aggtaagccg ggccgaggtt ctgcggctca
     6121 taccggcggc gttacggggc actctctcaa agcgtcggaa ggtgcatact gcccgcgcac
     6181 gggaggtcga gatagaactc tccgaagaag taccggcgca cgtagacggt gagatgcttg
     6241 cgcgcacccg ccacttcaag gcgcaggtac tgcccggaac cctacgaatc ctgacaccat
     6301 agcccatgga accaggtgac gagcgcccga gatcgacagg gaagagctac gaacaggcgt
     6361 gccgggatct cgaagtcgca gagcactatc aaaacgtaca ttgttgtggt acaacgtttc
     6421 tctactcctt cagggtagga ttgtatctcc gagtgatatc gaggactggc gagcagagat
     6481 gagggcgttc gtgtttccgg ggcagggggg cggagtagca gaacccgagc ccgagatggt
     6541 gccggagatc cagcgcgtcg tcggggagcg catacccgaa caggtcaaga tcttcgtccg
     6601 cgcggcgcgc gacgccgacg agagccgcac tatgaatgtg cgtcccgacg tggtggccgg
     6661 gcactcgctg ggcgaatacg gcgcggcgta cgcggctggt tgcttcgact tcgacactgg
     6721 cctctggctt gtggcgcagc gcgaccactt cctctccgaa gcggccagag agtgtcctgg
     6781 cgggatggtc gcgatactaa agactgaccc cgccgaggtc gagaaggcgc tcggcgcgcc
     6841 tggtggccct gtggtcgcca actacaactc acctcgccag acggtagtct ccggactgcg
     6901 cgacgccctc ggggacgccg tctccaagat cagaggacgc aagatcccgc tgaacgtctc
     6961 cttcgccgcc cactctccgt acgtggcggc ggccggtgag aagatgcggg gggtcctcga
     7021 ttccgtcgag ttccacgcgc cacaggtccc gatcgtcagc gccatggatg gtgctgtgct
     7081 caccaaggcc gacgccgtca agggggcgct caaggatcag atggtcgccc cggtgcgttg
     7141 ggtcagggtc gtcgagacgc tggcaaagat gagggttgag gagtggatcg agctcggtgg
     7201 cagcggcgtc ctgacgcgaa tgctcaaaga cttcgagttg ccgtctctga acggcatcac
     7261 cttcgaggac ctgcgctccc gcctctacgg acaggcccag aacctcctgc cgcgcaagga
     7321 aggcgaatag gttacagtct tcaggcggta cgctgtcgcg cacgcatcac gtgtagaaaa
     7381 gccctacctg aaggctgaca cctctaacct acctcgtcgc ctcggcctct gtctcggccc
     7441 tggtttcctc cttcgtgact gcgccgccgg agatctggac tatgtcctcc tcgatggatg
     7501 agatggggcc gtccatactg gaccatacgc cgatagagaa gccctcatcg acaggctcgc
     7561 ctatcttctg cccgaaggag acctcgtccc cctcggagac gagcgcagtt gcaggcttga
     7621 ggaacctgcc gccgagcggg atgctcaccg cgctgacctt gcccaccagc gaggtgacgc
     7681 cctcggggcg cctggtcttg atgttcgcgt actccttgga ggcgacgccc ttggggatga
     7741 cgaggaagcc gttcgtggtg cgcctgacgt aggcgtcact ctcgcctagc gactctatcc
     7801 ccatctcgtt caggtatggc ccgccgtcga taacagagag gttgccggct tcggcctcgg
     7861 cgtccacgcc agagagcttc aagagctccg tggcggacga cccgagcggc gcctcgaaga
     7921 gctcgagttc cagctcggga ccgaagacct ggaggtattt ggtcgtcacc ggcttgccaa
     7981 ggaagaacgc gttgtagatg ttcaggagcg tctcggtgtt gtttacgatg tgcccggcgt
     8041 cgagcggaag accgggtcgc ttgttgccgt cgggcaactc tacacggcgc ggcacccact
     8101 tccccttgtc ctcgttgaag atgttcttga tgagcgtctt ctcctcgccc atcgagtact
     8161 tgtca
//
LOCUS       DASTKM010000309         4054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_479329, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000309 DASTKM010000000
VERSION     DASTKM010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479329"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..851)
                     /locus_tag="VFQ10_12255"
     CDS             complement(252..851)
                     /locus_tag="VFQ10_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9966160.1"
                     /translation="MDVDMGRTSRVGRSKTPAADRILRAASEMFYREGIRAVGVDAIA
                     AGADVTKKTLYEKFGSKDRLVAAYLRARDERWRSWLVGVVERRGGTAGERLLSTFDAL
                     EEWMERENPRGCGFVNAAAELPHADHPARVVVMEQKMWMREYLAELAVEGGADNPEDL
                     AERLVILHEGATVAYSLGMAEDAAQKARQIAAHLVADLQ"
     gene            985..1869
                     /locus_tag="VFQ10_12260"
     CDS             985..1869
                     /locus_tag="VFQ10_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9966161.1"
                     /translation="MSAGIGKRRHLTEAGFVVMWSSGFIGASLGTPEAGTPTLMVWRF
                     LLAAGLLLVGILLLRRRWPGLREIAVQGVVGLLAQGVYLTGVVGAVEFGVTAGTTALI
                     AALQPLLAAALAGPVLGEHVGRQQWAGLAVGLVGVALVVGGDVRVGAEAPLWAYVMPF
                     VGMAGLVAATLVERRENLRRSALEAPMDVVLGIQCAASALLFAGLALFWGGLEPAGGL
                     LFWGAVAWFVVFSTFGGYGFYWLNLKLSSVARVSSLIYLTPPTTMVWAYLMFGERVGL
                     LAVVGLVICFGGVLLARD"
     gene            complement(1877..3133)
                     /gene="solA"
                     /locus_tag="VFQ10_12265"
     CDS             complement(1877..3133)
                     /gene="solA"
                     /locus_tag="VFQ10_12265"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HET9966162.1"
                     /translation="MNVRYSGRYDVIVVGVGGMGSATAYHLARRGRRVLGLERFGIPH
                     AMGSSHGHTRIIRLAYYEDPSYVLLLRRAYELWREIQGKAGEKLLHITGSIDAGPEES
                     WVFKGSWESCKLHDLPHEVLTGAELGRRYPGYHLPPEHLALIQPEGGFLTPERCIVSY
                     VMAAQSLGAEIHGHERVLEWVPLEGGVRVRTDRGTYEADRLVMTAGAWNGDLLDVLDG
                     LAVPERQVLAWLQPVRPDLFRPDTFPVFNLLVDEGRFYGFPVFGVPGFKFGKYFHLGE
                     SGGAEEIDRQPHDYDEQLLRSFAERYFPDGCGPTMDLQTCMFTNTPDHHFVIDLHPDY
                     PQVSIASPCSGHGFKFASVVGEIMADLAEKGFTRHDIGLFRLDRLRRQATGYQSSQPT
                     SAGQPAEQPDGRPRSFTRMGDIRTFW"
     gene            complement(3133..>4054)
                     /locus_tag="VFQ10_12270"
     CDS             complement(3133..>4054)
                     /locus_tag="VFQ10_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HET9966163.1"
                     /translation="EHAVGSSAYVTDMTAAVALPVVQGPRSRAFLKTLVEGVDLDELR
                     FFRFAPCRIGDVEVLISRSGYTGELGYELYTPADQAPPLWEHILQASQGFNLKPYGLE
                     AMQSLRIEKSLPLYGPDISEEYTPFHVGLGRWVRFEKRDFVGREALLRVQERGLQRRW
                     VGLTLESEAPAAAGAGVYGIADVATFREVVETGAEAGEHEDEFMPGERQVGRVTSSAM
                     GHTVGRMLAMAYVDTAHSWPGNNLIVELGGRPIPARVAQTPFFDPENARVRARSDEDD
                     KLSELVPEPARLTNARPRTPVRQSPNGNTR"
BASE COUNT          673 a         1283 c         1318 g          780 t
ORIGIN      
        1 gcggactacg gcgcacataa gcgcagatcc tacactacct tatgtatccc ggctcggtcc
       61 acccggcgct cggagactcc gcgctactcg cccggctcgt gcctccgccg gtagagcctg
      121 accctggaga tcgtgccgca caccctgcct tccttaccat cgttgtgtgt aagatggcta
      181 gcaaagacac cgaagagcgc cgcgtaggcg tggtctcggg tttgtgagga ggaacctggt
      241 ccgcttcctt actactgaag atcggcgacg agatgtgcgg cgatctgcct ggccttttgc
      301 gcagcgtctt cggccatccc caacgagtag gcgacggtgg ctccctcgtg gagaatcaca
      361 agccgctctg ccaaatcctc cggattatcg gcgccaccct cgacggcgag ctcagccagg
      421 tattcacgca tccacatctt ctgctccatc accaccaccc gcgcgggatg gtcagcgtgt
      481 ggtagctccg ccgcggcgtt cacgaaaccg caacccctgg ggttctcgcg ttccatccac
      541 tcctcgaggg catcgaacgt agagagcagt cgctcccccg cagtgcctcc ccgacgctcc
      601 acaacgccca ccaaccacga ccgccaccgc tcgtcccgcg cgcgcagata ggctgccacc
      661 agcctgtcct tggagccgaa cttctcgtag agggtcttct tggtgacgtc ggcccccgca
      721 gcaatggcgt cgactccgac agcccttatg ccttcccggt agaacatctc agaggcggcc
      781 cgcagtatgc ggtccgccgc cggtgttttc gaacgtccca ctctactcgt gcgtcccatg
      841 tctacgtcca ccttcgtctt cggtgttcct ctcacaaagt ataccaacca gtatgtttac
      901 aaaccaggtc taatcagtta ctatacatac cggtctgttt actaattgga agagactacg
      961 acgcgaatgg agcgtggtac cttcatgagc gctgggattg gcaagcgccg ccatcttacc
     1021 gaggccggct tcgtcgtgat gtggagctcg ggcttcatcg gcgcgagtct tggcacgccc
     1081 gaggccggta ccccgacgct catggtctgg aggttcctgc tcgccgcggg gctgcttctg
     1141 gtaggcatcc tcctgctccg gcggcgatgg cctgggttga gggagatagc cgtgcagggt
     1201 gttgtggggt tactcgcaca gggcgtatac ctgacgggtg tggtcggggc ggtggagttc
     1261 ggagtcaccg cgggcaccac ggccctcatc gccgccctcc aacccctctt ggccgcggcc
     1321 ctcgctggtc ccgtattggg tgagcacgta ggcaggcagc agtgggcagg actggccgtt
     1381 ggcctcgtcg gggtcgcctt ggtggttggc ggcgacgtac gggtgggtgc cgaggcgccg
     1441 ttgtgggcct acgtcatgcc ctttgtgggg atggctggcc tggtggcggc cacgctggtg
     1501 gagcgaaggg agaatctgcg aagaagcgct ctcgaggcgc ctatggacgt ggtgttgggg
     1561 atccagtgcg ccgccagcgc cctgttgttc gctgggctgg cactgttctg gggtgggctg
     1621 gagccggcgg gtgggttgct attctggggt gctgtcgcct ggttcgtcgt tttctccacg
     1681 tttgggggtt acggcttcta ttggctgaac ctgaagttga gcagcgtggc cagggtgagc
     1741 agcctcatct acctaacacc cccaaccacc atggtctggg cctacctgat gttcggagag
     1801 cgcgtgggtc ttctcgctgt tgtggggctc gtgatctgct ttggcggcgt cctgttggcg
     1861 agagactagc ccgcggctac cagaacgttc ttatgtctcc catcctggta aaagagcgag
     1921 gacgaccgtc gggctgctcc gccggttgcc cggcgctggt tggctggctc gactgatatc
     1981 cggttgcctg tcgcctcagg cggtcgaggc ggaagaggcc gatatcgtga cgggtaaacc
     2041 ctttctcggc caggtcggcc atgatctcac ctacgacgct ggcgaacttg aagccgtgcc
     2101 ctgagcaggg cgaggcgata gagacctgag ggtagtcagg gtgcaggtct ataacgaagt
     2161 ggtgatccgg tgtgttggtg aacatgcagg tctgcaaatc catcgtcggg ccgcagccgt
     2221 cggggaagta tcgttcggcg aagctcctga ggagctgctc gtcgtagtcg tggggctggc
     2281 ggtcgatctc ctcagcaccg ccggactcgc ccaggtggaa gtacttgccg aacttgaagc
     2341 cgggcacgcc gaaaacaggg aagccgtaga aacggccttc atccacgagc aggttgaaga
     2401 cagggaaggt atcaggccgg aaaagatccg gcctgaccgg ctgcagccag gcgaggacct
     2461 gcctctctgg cacggcgagc ccgtcgagga cgtcgaggag atccccattc caggcaccgg
     2521 cggtcatcac gagcctgtcg gcctcgtaag tgccgcggtc ggtgcggacg cgcactcctc
     2581 cctcaagcgg gacccactcg aggactcgct cgtggccgtg gatctcggcc cccagggatt
     2641 gcgccgccat cacgtaggag acgatgcacc gctccggcgt taggaaacct ccctcgggct
     2701 ggatgagcgc gaggtgctca gggggaagat gatagccagg atagcgccgc ccgagctccg
     2761 cgcctgtcag tacctcgtga gggaggtcgt ggagcttgca ggactcccac gagcccttga
     2821 agacccagga ttcctccggt ccggcatcaa tggagcccgt gatgtgcagg agcttctcgc
     2881 cggccttgcc ctggatctca cgccacagct cgtaggcgcg cctgaggagc agcacgtagg
     2941 aggggtcctc gtagtaggcc aggcggatga tgcgcgtatg accgtgtgag gaacccatcg
     3001 cgtgcgggat gccgaaccgc tccagaccaa gcacccgcct gcccctccta gcgaggtggt
     3061 aggcggtggc gctgcccatc ccccccacgc ctacgacgat aacgtcgtag cgaccggagt
     3121 accgaacgtt cactatctgg tgttcccgtt cggagactgt ctcacaggcg tcctgggtct
     3181 ggcgttcgtc agccgcgccg gctcggggac cagttcggag agcttgtcgt cttcatcaga
     3241 tctggccctg acacgggcat tctccgggtc gaagaagggc gtctgggcga ccctggcggg
     3301 gatgggcctg ccgccaagct cgacgatcag attgttgccg ggccaggagt gggcggtgtc
     3361 cacgtaggcc atggcaagca tcctaccgac ggtgtgcccc atggcgctcg aggtgacgcg
     3421 ccccacctgc cgttcgccgg gcatgaactc gtcctcgtgc tcgcccgcct cggccccggt
     3481 ctccacgact tccctgaagg tggcaacatc agcgatgccg tagaccccgg cgcccgctgc
     3541 ggcgggggcc tcgctctcga gagtcaggcc gacccagcgt ctctgcaggc cacgttcctg
     3601 gacccgcagc agggcctctc ggccgacgaa gtcgcgcttc tcgaatcgga cccaccgacc
     3661 cagacccacg tggaaggggg tgtattcctc gcttatgtct gggccgtaga gaggaaggct
     3721 cttctcgatg cgcagagact gcatggcctc gagtccgtag ggcttcaggt tgaacccttg
     3781 agatgcctgc aggatatgct cccacagcgg gggggcctga tccgcgggcg tgtatagctc
     3841 gtagccaagc tctccggtgt aacccgaacg tgagatgagc acctccacgt ccccgatccg
     3901 gcacggcgcg aaccggaaga accttagctc gtcgaggtcc acgccttcga ccaaggtctt
     3961 gaggaaagca cgcgaacgcg gtccctggac cacgggcagg gcgacggccg ccgtcatgtc
     4021 cgtcacgtag gcgctggagc caacggcgtg ctcg
//
LOCUS       DASTKM010000310         5213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_128666, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000310 DASTKM010000000
VERSION     DASTKM010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128666"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VFQ10_12275"
     CDS             <1..926
                     /locus_tag="VFQ10_12275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET9966164.1"
                     /translation="NTIDPLSKFTGIELPQGPPGGRQLRIANRAEWIDFNIEGFGRLM
                     EPVVQRAAAGAGDLTWAVGGVTLTAQVGLLLGFLSSRVLGQYDTGPLLTPGRAGEPGK
                     VFFLDGNIVSAAGRLGVPVDGLRLWIVLHEMTHALQFEGYPWLRRHLGELMESLISPL
                     AERLGLREMLRRLSGNMRTGGRSVELLMSRSQRESFDRMQAAMSVIEGYSDYVMHHVG
                     KGLVPHYEDLKARMAKSRAHRPPLETAVFRLTGLDVKLEQYRLGEAFADAVVKRQGME
                     GLNRAWERPENLPSLEEVRDPGLWMSRLGPS"
     gene            913..1257
                     /locus_tag="VFQ10_12280"
     CDS             913..1257
                     /locus_tag="VFQ10_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966165.1"
                     /translation="MDRPEMEEWSAPQSSTLGYEFAREEEARAAASDLDRRFTQDELA
                     GLRIYRVRWNGNYLVEAAFSDGTPEARLKDAVALLGESGIPVHPDDLADYKRATDEHT
                     SLPDWLRRFFGA"
     gene            complement(1482..1775)
                     /locus_tag="VFQ10_12285"
     CDS             complement(1482..1775)
                     /locus_tag="VFQ10_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966166.1"
                     /translation="MGQGTTKFVVGATVGAVGGFAVGAFASTTTARSARQSVLGGLDV
                     SARFLGRTVVQAVDGLGSVLESGYTRIRGREKYLEHEIDELREQIARLQQRID"
     gene            1863..3227
                     /gene="selA"
                     /locus_tag="VFQ10_12290"
     CDS             1863..3227
                     /gene="selA"
                     /locus_tag="VFQ10_12290"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HET9966167.1"
                     /translation="METGKRDLLRSLPAVDAVMRDPSGRTLAARHGRASATAAVREAL
                     GGLRRQIVAGERPEVSERVVAEAASRILTGRGLRRVVNATGVILHTNLGRSVLSERAA
                     LAAYDAATGYSNLEYDLRSGSRGSRYDHAVSLLRELTGAEDALVVNNCAGATLLALAA
                     VVASKATEVPEVIVSRGQLIEIGGGFRIPEVLTLSGARLREVGTTNRTRLSDYENAVT
                     ENTGAVLWVHPSNFETVGFTESVGVQELSSLGLPIIADVGSGALLPVGEEPRVQHTLR
                     DGADLAIFSGDKLLGGPQAGISVGASRWTSAMRHHPLARALRADKLCLAALEATLNSY
                     LDGTAEHDLPTLSMIRTPVPEIEQRATHLAENLRHNAPGLEVDVTPSVARSGGGTLPT
                     HEIPSFAVRLGGTDPEALAGNLRAGEPPVIGLVREGRLWLDVRTLLPGDEEAVIGALR
                     DARV"
     gene            3220..5076
                     /gene="selB"
                     /locus_tag="VFQ10_12295"
     CDS             3220..5076
                     /gene="selB"
                     /locus_tag="VFQ10_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HET9966168.1"
                     /translation="MFEAGEHRISLTIGTAGHVDHGKTTLVRYMTGTDTDRLEEEHRR
                     GISIVPGYAELDLPSGRRASLVDVPGHERFVKNMVSGATGVDAFLLVVAADDGVMPQT
                     IEHLDILRILGVEQGVVALSKVDAVDQETVELAALEVEELLESRDISAPVIHVSGATG
                     EGVEDLLLALDALAGETADRRKDDLARLPVDRSFVLKGIGVVVTGTLWSGEIRPGDVL
                     YTPTGLRPRVRGVQNHGHPAEVAYAGARTALDLTGVEASEIEAGDVLLSYPVPTSRDL
                     DVRVRLLEGAKPRAHGIRVRLHHGTRSTNARVRLTDREELEPGARALARLRLDEPLVT
                     LRGDRFVLRSMDGITIGGGTVLDPSATGRRLDPSWLEALESGDATKAVPLALAHRPGE
                     GMTTDELSLVLGFSADEVSRAVETSAGVETIGEIHAAASDISAARERLLEALERRAAE
                     RPESPELTIAEARTAMDLSTPLSDALLAEMEIEAVTVTDTGVSLTNAHRVPPELEREA
                     EQLLDALQAAGPQPPTAEPTPAMRLLLKRGDAVELGTSLFAARLVAESVLERIKTICR
                     EEGEISLAGLRDSLGTSRKYAQAWLEYSDTRGVTSRTGDVRVLTRRHRQAML"
     gene            5095..5184
                     /locus_tag="VFQ10_12300"
     tRNA            5095..5184
                     /locus_tag="VFQ10_12300"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5127..5129,aa:Sec,seq:tca)
BASE COUNT          958 a         1716 c         1692 g          847 t
ORIGIN      
        1 agaataccat cgacccgctc tcgaagttta cgggtatcga gctgccacag ggacctccag
       61 gcggccgcca gctaaggatc gcgaacaggg cagagtggat cgacttcaac atcgagggct
      121 tcgggaggct gatggagcct gtcgtgcagc gcgccgcagc cggagccggc gaccttactt
      181 gggccgtcgg aggcgtgacg ttgacggccc aggtagggct cctgctcggc ttcctctcct
      241 cgcgggttct gggacagtac gatacagggc cgctgctcac acctgggcgg gccggagagc
      301 ctggcaaagt cttcttcctc gacgggaaca tagtctcggc cgcaggaagg ctcggggtgc
      361 cagtcgacgg gctgcggctc tggatcgtgc tgcacgagat gacccatgcg ctccagttcg
      421 agggatatcc ctggctacgc aggcacctcg gggagcttat ggagagcttg atctcaccgc
      481 tcgccgagcg gctcgggctg cgtgagatgc tacgacgcct ctcgggaaac atgaggacag
      541 gcggacggtc ggtagagctg ctgatgagcc gctcccagcg cgagtccttc gacaggatgc
      601 aggctgccat gtcggtgatc gagggctact cggactacgt gatgcaccac gtgggcaagg
      661 gcctggtacc ccactacgag gacctgaagg cgcgcatggc caagagcagg gcccaccgcc
      721 caccgctcga gaccgccgtc tttcgcctga cgggcctcga cgtcaagctg gagcagtaca
      781 ggctcggcga agcgttcgcc gacgccgtgg tcaagcgcca ggggatggag ggcctgaacc
      841 gtgcctggga gaggcccgag aaccttccct cactcgaaga ggttcgcgat ccaggcctct
      901 ggatgtcgag gcttggaccg tcctgagatg gaggaatggt cagcgccgca atcctctact
      961 ctgggctacg agttcgcccg cgaggaggag gccagggccg cggcctcaga cctcgaccgg
     1021 cgctttacac aagacgagct ggcaggcctt cgcatctacc gcgtccgctg gaacggcaac
     1081 tacctggtcg aggccgcatt ctccgacggt acacccgagg cgcggctgaa agacgccgtc
     1141 gccctcctcg gcgagtctgg catcccggta caccctgacg atctcgctga ctacaagcgg
     1201 gccacggacg aacacacaag cctgcccgac tggctgcgcc ggttcttcgg cgcttgaaag
     1261 cacgctccgg cccttagggc ctacgctcgt cagcacgcta cgggcctttg aggccctccc
     1321 ttgtcagcat ctcagcttct cccgttccga accttgccct gccgcgtacg acgaaccgct
     1381 accagcggca atcgggttct acgctcttgg cgaagctaca gaacgacttg ctaaaatgct
     1441 gacaagcgcg ggctttggaa gggcaaaccc ttcaaacccc gctaatctat tcgttgctgt
     1501 aggcgtgcga tctgctccct gagctcgtct atttcgtgtt cgagatactt ctcacgacca
     1561 cggatgcggg tataaccgga ttccagaacg gacccgagac catccacggc ctggaccacg
     1621 gtccttccca agaaacgtgc ggagacgtcc agcccgccga gcaccgactg gcgggccgac
     1681 cgcgcggtcg ttgtgctcgc gaaggctccg acggcgaaac cgccgacggc cccgacggtc
     1741 gccccgacaa cgaatttggt ggttccctgg cccaaaagca gcttccttcc ggctcttccg
     1801 agcttcgacg gcgcaagttt agcacagcgc ccatgctgca aggttatact tggcggcgca
     1861 tgatggagac ggggaagagg gatctactaa ggagcctccc cgcggtagac gccgtgatgc
     1921 gcgacccgtc gggacggacg ctcgccgccc gacacggcag ggcttcggcg acggcggccg
     1981 ttcgcgaggc cctcggaggc ctgcggcgcc agatcgtcgc gggagaaagg ccggaggtct
     2041 cggagagggt cgtcgcagaa gcagcctcta ggatcctaac aggtagaggt ctgaggcgcg
     2101 tcgtcaacgc aacaggcgtg atccttcaca ccaacctggg gcgctcggta ctctccgagc
     2161 gggcggctct ggcggcctac gatgcggcca ccggctactc caacctggag tacgatctcc
     2221 gctccggctc tagaggctcg cgctacgacc acgcggtgtc gctcctcagg gaactgaccg
     2281 gcgccgagga cgcgctcgtc gtcaacaatt gtgcgggggc gacgttgctt gcgctagccg
     2341 cggtcgtcgc ttcgaaggcc actgaggtcc ccgaggtgat cgtctctcgc ggccagctga
     2401 tcgagatcgg gggcggattc cgaatcccag aggtgctcac cctctccggc gcgcggctca
     2461 gggaggtcgg aaccacgaac cgtaccaggc tctcggatta tgagaacgcc gtaaccgaga
     2521 acaccggcgc ggtcctctgg gtccatccca gcaacttcga gacggtcggc ttcacagagt
     2581 cggtaggggt ccaggagctc tcgtctcttg gactcccgat catcgctgat gtcggcagcg
     2641 gtgcgctctt acccgtgggt gaagaaccac gggtgcagca taccctgaga gatggcgctg
     2701 acctcgcaat cttctcggga gacaaactcc tcggcgggcc gcaggcaggg atatcggtag
     2761 gagcttcgcg ttggacctcc gccatgcgcc accatcccct cgcccgcgct ctccgcgccg
     2821 acaagctgtg cctcgccgcc ctggaagcga cgctgaactc ttatctcgat ggcaccgccg
     2881 aacacgatct cccgaccctc agtatgatac gaaccccggt ccccgagata gaacaaaggg
     2941 ccacccacct cgccgagaac ctcaggcaca acgcgcctgg cctcgaggtg gacgtgacgc
     3001 cttctgtagc ccgcagcggc gggggtacgc tgccgacaca cgagattccc tccttcgccg
     3061 taaggctggg cgggaccgac cctgaagccc tggccgggaa cctgcgcgcc ggagagccac
     3121 ccgtaattgg ccttgtgcgt gaggggaggt tatggctgga cgtacggacc ctcctgcccg
     3181 gtgacgagga ggccgtgatc ggggcactca gggacgcgcg tgtttgaggc aggcgagcac
     3241 aggatctccc taaccatagg gacggcgggg catgtggacc acggcaagac cacgctcgtc
     3301 cgttacatga cgggcacgga taccgaccgt ctggaagagg aacaccgcag gggaatctcc
     3361 atcgtgccgg ggtacgcgga gcttgacctt ccgagcggaa ggcgggccag cctggtcgac
     3421 gtgcccggtc acgagcgctt cgtcaagaac atggtctcgg gggccactgg ggtcgacgcc
     3481 ttcctgctcg tcgtcgccgc cgacgacggc gtcatgcccc agacgatcga gcacctggac
     3541 atcctgcgga tcctcggtgt cgagcagggg gtcgtcgcgc tctcgaaagt cgatgccgtg
     3601 gaccaggaga ccgtggagct ggccgccctg gaggtcgagg agctgctgga gtcgagggac
     3661 atcagtgctc ctgtaatcca tgtgagcggc gcgaccggtg agggcgtcga ggatctgctg
     3721 ctggcgctgg acgcgttggc cggggaaacg gcggacaggc gcaaggacga tctggcacgc
     3781 ctgcccgtgg accgctcttt cgtgctcaag ggtatagggg tcgttgtgac ggggacgttg
     3841 tggagcgggg agattcgtcc aggcgacgtt ctgtacactc ctacgggcct gcgcccgcgg
     3901 gtgcgtggcg tacagaacca cggtcacccc gccgaggtcg cctacgccgg ggcacgtacg
     3961 gccctcgacc tcacgggcgt cgaagcctcg gagatcgaag cgggtgacgt cctgctctcg
     4021 taccccgtgc ccacgagccg cgatctcgac gtccgcgtcc gcctccttga gggcgcgaaa
     4081 ccccgcgccc acggcatccg cgtaaggctg caccacggga cacgttccac gaacgcccgc
     4141 gttcgcctga cggaccgcga ggaacttgag cctggagcca gagccctcgc ccgcctgaga
     4201 ctcgacgagc cgttagtcac gctcaggggc gaccgcttcg tgctgcgctc catggacggg
     4261 atcactatcg gcggcggcac cgttctcgac ccatcagcga cggggcgccg cctcgatcca
     4321 tcatggctcg aggccctcga aagcggcgac gctacaaagg ctgtcccact cgccctggcc
     4381 cacagacccg gtgagggcat gaccacagat gaactctcgc tggtcctagg attctcggcg
     4441 gacgaagtgt ctcgagcggt cgaaaccagc gccggggtag agaccatagg ggagatccac
     4501 gccgcagcca gcgacatctc agcggcgcga gagcggcttc tcgaagcctt agaacgtagg
     4561 gccgccgaac gtcccgagag ccccgaactc accatcgccg aggcccgcac agcaatggat
     4621 ctttcgactc cgctctctga cgccctgctc gcagaaatgg agatcgaagc ggtaacggtg
     4681 accgatacag gcgtcagtct gacgaacgcg cacagggtgc cccccgagct ggagcgcgag
     4741 gccgagcagt tactcgatgc cttgcaagcc gccgggccac aacctcccac ggcagagccg
     4801 acaccggcga tgcgtctgct cctcaagcgc ggggacgccg tcgagctagg gacctcgctc
     4861 ttcgccgcgc gtctggtagc cgagtcggta ttagagcgga taaagaccat ctgcagggaa
     4921 gaaggtgaga tctcacttgc cggactccgc gacagcctcg gcaccagccg caagtacgcc
     4981 caggcctggc tcgaatactc cgacacccgc ggcgtcacca gccgtacggg cgacgtaaga
     5041 gtcctcaccc gccgccacag gcaggcgatg ttataaactc tacccgccga accaggaatg
     5101 cgtgggcctg gtggcccggc cggtcttcaa aaccggtttg cgaccgtaag tgcggccgtg
     5161 gcggttcgat tcccccgcat tccgcgaaaa aagcaagtca gcaagccgct tgc
//
LOCUS       DASTKM010000311         3376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_659392, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000311 DASTKM010000000
VERSION     DASTKM010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659392"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..631)
                     /gene="aat"
                     /locus_tag="VFQ10_12305"
     CDS             complement(59..631)
                     /gene="aat"
                     /locus_tag="VFQ10_12305"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET9966169.1"
                     /translation="MKLTTEILEACYRAGAFPMADAFGRIGFYRSNPRSVLEFEDLHV
                     PRSLGRVIRKETFEVRVDEDFDGVIRACADRKETWINSEIIDAYIRLHHADKAHSVEA
                     YAGGELAGGLYGVTLGGAFMGESMFTRIRDASKVCLVYLVNRLQERGYELLDCQIQNP
                     HLARLGAREIPEHEYLRRLQHALSLECSFV"
     gene            complement(849..1865)
                     /locus_tag="VFQ10_12310"
     CDS             complement(849..1865)
                     /locus_tag="VFQ10_12310"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET9966170.1"
                     /translation="MSSQVPGDAGLPEDAPLATVTRGPLVESVHRGRLAICDPDGKVL
                     QAVGDPEASIYARSSAKPFQALPLVLSGAADAFGLTDRELAVVCASHNAEEPHIAAVK
                     SILEKAGLMEDDLQSGAHLPLHEPEAERLIRRGEEPRPIHGNCSGKHAGMLAVCVYEG
                     YETATYRDPAHPLQRRILGLIAQVCGLREDEVLVAGDNCGVPAFALPLRSFATGLARL
                     ATGNDLPDELALAALRIGGAMRAHPFMVAGTGRFDTDLMVSTDLIVKGGAEAVLAAGS
                     QDGWGMALKISDGSVRAVRPAALAALGGMGVEVPEAVSDVRGLHDEKVGEIRPVTQVH
                     TDSA"
     gene            complement(1858..2181)
                     /locus_tag="VFQ10_12315"
     CDS             complement(1858..2181)
                     /locus_tag="VFQ10_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966171.1"
                     /translation="MVPVKAGERSGAALRTAHLLRIDSYMDTAIIAMWTASPRTDTML
                     GMVEASLRGGSPCGEDDELIEKLRALVREGREYLAGDEFSVAMGRMRVAHDLLSLHII
                     RLSDE"
     gene            complement(2169..2984)
                     /locus_tag="VFQ10_12320"
     CDS             complement(2169..2984)
                     /locus_tag="VFQ10_12320"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9966172.1"
                     /translation="MEYDIEALTGGALASIWSWVQGKIPAGVEIFDAHAHIGTDIDGR
                     TMTGDGMRDRMEAAGVKKSIVFPLNDPNARDDYSGPNDVVWNAYEAFPSYFVPFFRLN
                     PHLDYDREFGRCVERGFMGLKLHPVSQKFELDDLRVVRLFGMAAAIDLPVLIHAGFAM
                     QRIVEPLLPTVDTHPGLRLILGHSAMVEVLCAARAFEGHPNVLFETSVVQAKDLYELC
                     SSIDSSRIVYGSDIPYGDLPSTLHAILAAAEAAGVPEEALPGILSENIRRWFP"
     gene            complement(3208..3375)
                     /locus_tag="VFQ10_12325"
     CDS             complement(3208..3375)
                     /locus_tag="VFQ10_12325"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HET9966173.1"
                     /translation="MPHEDFDTIGGYILGSLGRPPESGDTVEADGVTMQVKSVDGPRV
                     SMLTLQRNEDS"
BASE COUNT          595 a         1065 c         1101 g          615 t
ORIGIN      
        1 tcgtatatcc atggggctag cttaccacgc ggctagcgcc gacccgaaag tgacccctct
       61 atacaaagga gcactccagg ctcagggcgt gctgcaagcg ccgcaggtac tcgtgctcgg
      121 ggatctcacg cgcccccagc cgggcgaggt gggggttctg gatctggcag tcgagaagct
      181 cgtaaccgcg ctcctgcagc cggttcacca ggtagaccaa gcagactttg gaggcgtccc
      241 ttatgcgggt gaacatcgac tcgcccatga aggcaccgcc aagagtgacc ccgtagagtc
      301 cgcctgcaag ctcacctccg gcgtacgcct cgacgctgtg agctttgtct gcgtggtgca
      361 gcctgatgta agcgtcgatg atctccgagt tgatccaggt ctccttcctg tcggcgcacg
      421 cccttatgac accatcgaag tcctcgtcca cacgcacttc gaaggtctct ttgcggatca
      481 cacgccccag tgagcggggg acgtgcaggt cttcgaactc cagcacgctt cgagggttcg
      541 agcggtagaa cccgatgcgc ccaaaggcat ccgccatcgg gaaagcgccg gctctgtagc
      601 acgcctcgag gatctccgtc gtcagcttca tgggcctatg gtacagtgac tcgggcctac
      661 ccggacggca gtcgcccgtc cgacaccatc tggtctacgt catccatccc tgatgcgtaa
      721 accggcagat cgcttgatgt tggaagtaca aagatcttgc cgcagtggat tcccgcttgc
      781 gcgggtatga cgctttcatg ctgggcgtaa ggggatatga gttgcgagac cgtacaccgc
      841 ggaagtgatc acgcggagtc tgtatgaacc tgggtcacag gcctgatctc accgaccttc
      901 tcgtcgtgca ggccacgcac gtcggagacc gcctcgggca cttcgacgcc catgccgccc
      961 agagcggcga gcgccgctgg ccgcacggcc ctgaccgagc cgtctgagat cttcagtgcc
     1021 atgccccagc cgtcctggct cccggcggcg agcacggcct cggccccgcc cttgactatg
     1081 aggtcagtcg agaccatgag gtccgtgtcg aaccttcctg tgcccgcgac cataaaggga
     1141 tgcgcccgca tcgcgccccc tatgcgcagg gccgcaagcg cgagttcgtc ggggaggtcg
     1201 ttccctgtgg cgaggcgggc gaggcctgtt gcgaagctac gcagcggcag ggcgaaggcg
     1261 gggacgccgc agttgtcgcc ggcgacgagc acctcgtctt cacggaggcc gcaaacttgg
     1321 gcgatgagac cgagtatgcg gcgttggagc gggtgggcgg ggtcgcggta tgtggcggtc
     1381 tcgtagccct cgtagacgca gacggcgagc atccccgcgt gcttgccaga gcagtttccg
     1441 tggatcggac gcggctcttc gcccctgcgg atcagccttt cggcctcggg ttcgtgcagc
     1501 ggcagatggg cgccgctctg caggtcgtcc tccatcaggc cggccttctc caggatagat
     1561 ttgacggcgg cgatgtgagg ctcctcggcg ttgtgcgagg cgcagactac ggcgagctct
     1621 ctgtccgtca acccgaaggc gtcggcggcc cccgagagga cgagcgggag cgcctggaac
     1681 ggcttggccg aggagcgggc gtagatggag gcttcgggat cgccaacggc ctggagtacc
     1741 ttgccatcgg ggtcgcagat ggcgagacgc ccccggtgga cgctctcgac gagcgggcca
     1801 cgcgtgacgg tggcgagggg tgcgtcttcg ggcagaccgg cgtcgcctgg gacctggcta
     1861 ctcatccgat aggcggatga tgtggagaga cagcagatca tgggccaccc gcatacggcc
     1921 catcgcgacg gagaactcgt cgcctgccag gtactcgcgt ccctccctga cgagggcgcg
     1981 gagcttctcg atcagttcgt cgtcttcgcc acagggactc ccaccccgca ggctcgcttc
     2041 gaccatgccg agcatggtgt cggtcctcgg gctcgctgtc cacatcgcga tgatcgcggt
     2101 gtccatgtag ctgtcgatcc tgagcaggtg cgccgtcctc agcgccgcgc cggacctctc
     2161 ccccgccttc acgggaacca ccgcctgatg ttctccgaga gtatcccagg cagggcctcc
     2221 tcaggcaccc cagcggcctc ggccgccgcg aggatggcgt gcagcgtcga tggcaggtcg
     2281 ccgtagggta tgtccgaccc gtaaacgatg cgggaagagt cgatagaaga gcagagctcg
     2341 tacaggtctt tggcctgcac gacggaggtc tcgaacagta cgttcgggtg cccctcgaag
     2401 gcgcgcgcgg cgcagagcac ctcgaccatc gccgagtggc cgaggatcag gcgcagtccg
     2461 ggatgcgtgt cgactgttgg aagcagcggc tcgacgatcc tctgcatggc aaagccggca
     2521 tggatgagca ccggtaggtc tatagccgcc gccatcccga acaggcgcac gactcttagg
     2581 tcgtcgagtt cgaacttctg cgagacggga tggagcttga gacccataaa gccgcgctcc
     2641 acgcagcgcc cgaactccct gtcgtaatcc aggtgcgggt tgagccgaaa gaagggtacg
     2701 aaataggatg ggaacgcctc gtaagcgttc cagacgacat cgttcgggcc cgagtagtcg
     2761 tcgcgggcgt tagggtcgtt cagggggaag acgatgctct tcttcacccc tgcagcctcc
     2821 atgcggtcgc gcattccgtc tccggtcatc gtcctgccgt cgatgtcggt cccgatgtga
     2881 gcgtgggcgt cgaagatctc gacgccagcg gggatcttcc cctgcaccca ggaccagatg
     2941 gaggccaaag ccccgcccgt aagcgcctcg atgtcgtact ccatctgcaa agcgaccgcc
     3001 gatctccgac caacagccgt gaaatgttac cagggggggt tcgacgttgc taaagagagc
     3061 cgcacagggt tttcatgaga ggacttcgaa cgccggtctc gagaggcaac ccctctcgaa
     3121 cgtctctagc ctcattagcg gaacatcacc tcgtcgatca tccaattgcg agattcagat
     3181 tggtttcggg gtactctact gcagagccta ggagtcttcg ttccgttgga gggtgagcat
     3241 ggagacgcgt gggccgtcga cgctctttac ctgcatggta acgccgtcgg cctcgacggt
     3301 gtcaccgctc tcggggggac gtccaaggga gccgaggata tagccgccta tagtgtcgaa
     3361 gtcctcgtgg ggcagg
//
LOCUS       DASTKM010000312         6721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1013193, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000312 DASTKM010000000
VERSION     DASTKM010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013193"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..825)
                     /locus_tag="VFQ10_12330"
     CDS             complement(64..825)
                     /locus_tag="VFQ10_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET9966174.1"
                     /translation="MRQIKLTIHHLYADMMNLYGDRGNVISIKKRCEWRGIRVEVDDV
                     GLGERIKPTGCDLFLFGGGQDREQALLAEDLSGSKGTDLRSIVEDGGVVLGVCGGYQL
                     MGHSYETPEGEKLPGVGIFDLHTEPRKADEERMIGNVLVRVRAPETGETREVVGFENH
                     GGRTYLGEDVKPLGVVVVGYGNNGKDGTEGARRLNAYGTYLHGSLLPKNPWLTDQLIL
                     SALRQVDENFELEPLDDTVEGRAFSSVAGRVAGGR"
     gene            1146..1412
                     /locus_tag="VFQ10_12335"
     CDS             1146..1412
                     /locus_tag="VFQ10_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966175.1"
                     /translation="MGSVRGVREGISQEEKTEAMIRVGDRVRVIPHEVSPPTQKYASQ
                     KGYVTMTSPGTYGPQLFVQLDNNPDGIDTAFEEGDLEEISPLED"
     gene            complement(1480..1878)
                     /locus_tag="VFQ10_12340"
     CDS             complement(1480..1878)
                     /locus_tag="VFQ10_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966176.1"
                     /translation="MSEDFDIALRSILGQLVEAREDQNDADKRLHDYRATKTPPEIPN
                     EFEDVNAFLDYHHRRQSYENHLKQHENALNMAKREYTAAAEQLLVFLPDGVPLRYTYE
                     GERSELFGAEYVMVRKQGEIVIQSPSRATS"
     gene            complement(1976..3391)
                     /locus_tag="VFQ10_12345"
     CDS             complement(1976..3391)
                     /locus_tag="VFQ10_12345"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HET9966177.1"
                     /translation="MSPLPDPRLSAAILAARSARFASRSLKTGGGSTVPGVVARRVDP
                     RVLTKLSRRLRLGSAAITGTNGKTTTTRMVSKILQTAGVRAVNNTTGANLVTGVSAAL
                     VSDSGLAGTPLSQMGLFEVDEASVPKVAAEAEIEILAVLNLFRDQLDRYGELAYTGKV
                     IASAFGDLPQSGAVVLNSDDPLVASLGRSARRPVFYGVDEPALDTGRLQHVADSKDCP
                     VCGTALVYETVYMGHVGLYDCRHCNFSRPEPAYRASRVRLEGAKGSEFQLSTPKGEAD
                     VRIGLPGLYNVYNALAAAAVAGEAGVGLAEISRGLREFGGAFGRVERVQAGDREAFLL
                     LIKNPVGFNEILRTFVTGAEARNVLIAINDNDADGRDVSWLWDVDFEMLADAREEGKT
                     DAAPFTVSGIRAEDMAVRLKYADLPVGPVIPDRAEAIKSALAATPPGETLYVLPTYTA
                     MLEIRHTLTEMGYTHPFWEDR"
     gene            complement(3474..5627)
                     /locus_tag="VFQ10_12350"
     CDS             complement(3474..5627)
                     /locus_tag="VFQ10_12350"
                     /inference="COORDINATES: protein motif:HMM:NF039646.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HET9966178.1"
                     /translation="MTRLRSTDALVLLFSAAAVILLLPLRGLSVAFSPVAFAATLVLF
                     TAPGLLLSHWLLSDDMAGPTLVPVGFAASTGIFGLLGVPALILHLSTEDYILAAGTLL
                     AAFLAVAAWRTLRPKSPVAECSPEQEAMYGPPAGWLWAPFALLCGVLAFVGARRVPSS
                     YDDIWVYLSWVKYFADSERLALVDPYFGERIAEFSRAKVNGWLLEQAALSRVSGLDTI
                     ELVLRYLTPTLVVVALLMVYALAFAISKSERAAVLIASVYALFHIIFIQPSVHNVGVE
                     LAARISEDKYAARFLILPVTLLFALLFVERRRWRHLGLFTFFCWAAIAVHPSVLPAIG
                     LCMLGFGVAHVAANLRLRAAWTGMVALALGMWSVVLGPVILLFTGRSPEAVLFSADIN
                     ATPPKVLEYTVFITESWRHIYELGGGYYIMHPWLLLNPVILGTYVVGVPFLLWRVRTN
                     VAAQLLLGGLAVVTVAVYIPPVATFIGDRLIVPGLLWRLAWPIPLLALITTGWMVWDA
                     LTYAEARLRESGIRHSVIKVLPLALVALLTAAAAPPSVEKAVGLYRKFDVARTTNYDP
                     DPIYPWIRNSIEDPSVLLARDSANNVVPAYSASLNVVSQRGEGMIRDRDELEKLAGSR
                     INIPQRYLDVHSFFFGPTLDREAYGILRRYRADYLMVYADSPLDERLKTLPGFSPVEE
                     APREKYSLYNVDLEKLGKPARDSNRPRETAPLAPS"
     gene            5814..>6721
                     /locus_tag="VFQ10_12355"
     CDS             5814..>6721
                     /locus_tag="VFQ10_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9966179.1"
                     /translation="MEIFENLSASSRLEDAELDAYSRAVRDLTERLEPAVISVGLPDG
                     RGGGSGVILGLEGDSATAITNSHVVRGLRSRRAGNPGGDIQVTLSDGSSVSAEMLGDD
                     PPSDLAVVRFDTEEEPTVATLGDAGDLVVGQLVVAIGNPLGFQRSVTAGVVSALGRSI
                     RGESGRLIENVIQTDAAINPGNSGGPLADASARVVGINTAIIGGAQGIGFAVPVSGAF
                     RRVVFTLVTEGRVRRAYLGVVVESRQARGEFEGGAGVRNIAPNSPAERAGVKPGDVII
                     GLKGEPVHSNDDLLNLLDASSIGQDAT"
BASE COUNT         1296 a         2136 c         2091 g         1198 t
ORIGIN      
        1 gcaagataga accgcctgga ctctggagtt ggacctgcgg ccctcggtgg agagcttctg
       61 accctaccgc ccgccggcca cgcggccagc caccgatgag aaagcacgcc cctccaccgt
      121 atcgtccagg ggttcgagct cgaagttctc gtccacctgg cgcaaggcgc tcaggatgag
      181 ctgatccgtg agccaggggt tcttggggag taaggatccg tgcagatagg tgccgtaggc
      241 gttcagcctg cgcgcaccct cggtgccgtc tttaccattg ttgccgtaac ccacaacgac
      301 cacgccgaga ggtttcacgt cctcgccgag gtaggtgcga ccgccgtggt tctcgaagcc
      361 gacgacctcg cgcgtctcgc cggtctcggg tgcccgtacc cgaaccagca cgtttccgat
      421 catgcgctcc tcgtcagcct tcctgggctc cgtgtgcaag tcgaagatgc cgacgccagg
      481 aagcttctcg ccctcgggcg tctcgtagga gtggcccatg agctggtagc caccgcacac
      541 cccgagtacc acgccgccgt cctccacaat ggatctcagg tccgtgccct tggagccaga
      601 gaggtcttcg gccagcagcg cctgttccct gtcctggccg ccgccgaaga ggaagaggtc
      661 gcagccggtc ggctttatcc gctctccgag gccaacgtcg tcgacctcca cccgaatgcc
      721 gcgccactcg caacgcttct tgatggagat gacgtttccc ctgtcgccgt aaaggttcat
      781 catgtccgcg tacaggtggt ggatggtcag cttgatctgt cgcatgacac ctccctatcg
      841 ctgcgtaccg tctttggaca ggaggcgctc aacccgcgca cgacactcct ggcgccattt
      901 taccctgtcg gggtcgtaga tcatacgctc gaccaactga cgggtctcct ccgctggcga
      961 aaagaaacca ccggcggggt gcccggcgat ctcgacgcga ccatcgctgc agataccggc
     1021 cttcagcccc aatgcagtgc agatgcggcg gcgctcttca tctagctgac tcagccgcac
     1081 ccgcagttcc tcaagcgtcc tcagctatac agactccgcc gcagtctgtt aggagtagcc
     1141 gagcaatggg tagtgttaga ggagttagag aaggtatttc acaggaggag aagactgaag
     1201 cgatgatccg agtaggagac cgcgttaggg ttatccccca cgaggtatcg ccgcctactc
     1261 agaagtacgc cagccagaaa ggttacgtaa cgatgacctc tcctggcacc tacggtccgc
     1321 aattgtttgt ccagctcgat aacaatcccg acggtatcga tacggccttc gaggaaggcg
     1381 accttgaaga gatctcaccg ttggaagact aaactacaca gctgaaggcg gctccgtcgc
     1441 accacggatg acttgccgag ggcggatggt ggggcggagc tagctagtcg ccctgctggg
     1501 actctggatg acgatttcac cctgcttgcg caccatgacg tactctgcgc caaacaactc
     1561 gctccgctcg ccttcgtagg tgtagcgtaa aggcacacca tcgggcagga aaaccaggag
     1621 ttgctcggca gcggccgtgt actccctttt tgccatattc agggcgttct cgtgttgctt
     1681 gagatgattc tcgtagctct gcctgcggtg gtggtagtcg aggaaggcat tcacgtcctc
     1741 gaactcattg gggatttcag gaggcgtctt agtggccctg tagtcgtgga gccgcttatc
     1801 agcgtcgttc tgatcttccc gtgcttctac cagttggccg agaatgctcc gtagagctat
     1861 gtcaaagtct tcgctcaatt cctttactcc tcatctccac cgggcgtaga gaggccctct
     1921 gggatcatac tccccagcga ctcggcggcc tctgtgggcg aagcccgcaa acctttcacc
     1981 gatcctccca gaaaggatga gtgtagccca tctcggtcag agtatgccgg atctccagca
     2041 tggccgtgta cgtgggcagt acgtagagcg tctcgcctgg gggggtggcc gccagcgcag
     2101 actttatggc ctctgcgcgg tcggggatga cggggccaac aggaagatcc gcgtacttga
     2161 gccgtacggc catgtcttcg gccctgatcc cgctgacagt gaaaggggcg gcatccgttt
     2221 tgccctcctc cctcgcgtcg gcgagcatct cgaagtccac gtcccagagc cacgagacat
     2281 cgcggccgtc ggcgtcgttg tcgtttatgg cgatcaggac attgcgggct tctgcgccgg
     2341 tgacgaaggt gcgcaggatc tcgttgaacc caacagggtt ctttatgagc agcaggaagg
     2401 cctctctgtc cccagcctgt actcgctcga ccctcccgaa agccccaccg aactccctga
     2461 ggccgcggga gatctccgcc agcccgaccc ctgcctcccc ggccaccgcg gcagcggcga
     2521 gggcgttgta gacgttgtag agaccgggga gccctattct cacgtcagcc tcgcccttcg
     2581 gggtcgagag ctggaactct gagcctttag ctccttcgag ccgcaccctg ctcgcgcgat
     2641 aggctggctc cggccgcgag aagttgcagt gccgacaatc gtagaggcca acatgcccca
     2701 tgtatacggt ctcgtagacg agggccgttc cgcacacggg gcagtctttg gagtcagcga
     2761 cgtgctgcag acgcccggtg tccagagcag gttcgtccac gccgtagaag acgggtctcc
     2821 tggcagaacg cccgagactc gcgacgagag ggtcgtccga gttcaggacc accgcgccgc
     2881 tctgcggcag gtccccgaag gccgaggcta tgaccttgcc cgtataggca agctccccgt
     2941 agcggtcgag ctggtccctg aacagattca gtaccgcgag gatctcgatc tccgcctccg
     3001 cggccacttt cggcacgctc gcctcgtcca cctcgaagag ccccatctga gagagaggcg
     3061 tccctgccag ccccgagtcc gagacgaggg ccgcactcac accggtaacc aggttagccc
     3121 ccgtcgtatt gtttacggca cggacaccgg cggtctggag tattttggag accatcctgg
     3181 tcgttgtcgt cttgccgttg gtgcccgtaa tcgccgccga cccgaggcgc agccgtcgcg
     3241 agagcttggt caatactctt gggtccaccc tgcgcgccac tacccctggc accgtcgagc
     3301 ctccgccggt cttcagcgac ctgctcgcga accgggccga ccttgcggcc agtatcgcgg
     3361 cggagagacg cgggtccggt aaggggctca tggcgtggat tatatgattc tgggtggaat
     3421 cgtgcggaag gatctctcag tcagagatcc actcctacct gcgtcctgac tcctcaggat
     3481 ggcgcgagcg gagcggtctc gcgaggccgg ttggaatccc gggccggttt gcccagcttc
     3541 tcgaggtcca cattgtaaag gctgtatttc tctctgggtg cctcctctac tggggagaag
     3601 ccaggcaggg tcttcagccg ctcgtcgagc gggctgtccg cgtagaccat caggtaatct
     3661 gcgcggtagc ggcgcaatat gccgtaggcc tctctgtcca gggtggggcc gaagaagaag
     3721 ctgtgtacgt ccaggtagcg ttgggggatg ttgatgcggg atcctgccag cttctccagc
     3781 tcatcgcggt ctcgtatcat accctcgccc ctctggctca ccacgttcaa cgaggccgag
     3841 taagcgggga cgacgttgtt cgcgctgtcg cgcgcgagca gcacgctggg gtcctctatg
     3901 ctgttcctga tccagggata gatagggtca gggtcgtaat tggtcgttcg cgcgacatcg
     3961 aacttgcggt acagtcctac ggccttctct acggaaggcg gcgcggcggc cgccgtcagg
     4021 agggccacca gcgccagcgg caggaccttt atcacgctgt gtctgatccc ggactccctc
     4081 aagcgcgcct cggcgtaagt gagggcatcc cacaccatcc agccggtagt gatcagggct
     4141 agcaacggta ttggccacgc gagacgccag aggaggccag ggacgatgag acgatcccct
     4201 atgaacgtgg cgacaggggg aatgtacact gcgacagtca cgacagccag accacccagg
     4261 aggagttggg cggccacgtt cgttctcacc cgccacagca agaaaggtac accgaccacg
     4321 taagtcccaa gtatcacggg gttgagcagc aaccaggggt gcatgatgta gtagccgccg
     4381 cccagctcgt agatatgcct ccagctctcc gttatgaata ccgtgtattc gagcactttg
     4441 ggaggcgtcg cgttgatgtc agcggagaac agcacggcct ccggagacct gcccgtaaac
     4501 agtaagatca caggcccgag cactacgctc cacatcccca gcgcgagggc taccatcccg
     4561 gtccatgccg ccctcaggcg caggttggcc gccacgtggg ccaccccgaa gccgagcatg
     4621 cacagcccga tggccggtag tacagaagga tgcacggcta tcgcagccca gcagaagaac
     4681 gtgaagaggc cgagatgccg ccacctcctg cgctccacga agagcaaggc gaagagcagc
     4741 gtcacaggta ggatcaggaa ccttgctgcg tacttgtcct ccgagatacg ggctgccagc
     4801 tccaccccga cgttgtgcac ggaaggctgg ataaagatga tgtggaacag ggcgtagaca
     4861 gaggcaatga gtaccgcggc ccgctcgctc ttggagatgg caaaggccag ggcatagacc
     4921 atcaacaggg ccacaacaac gagcgtaggt gtgaggtacc tgagcaccag ttcgatcgtg
     4981 tccagaccag agacgcggga gagggctgcc tgctcgagca accatccgtt caccttcgcg
     5041 cgggagaact cggcgatcct ctcgccgaag taagggtcca ccaacgcgag cctctcggaa
     5101 tccgcgaagt acttcaccca cgacagatag acccatatat cgtcgtagct gctaggcacc
     5161 ctcctggctc ccacgaaggc cagaaccccg cagagcaaag cgaacggcgc ccacaaccag
     5221 ccggccggag gaccgtacat agcctcctgt tcgggagagc actccgccac gggcgacttc
     5281 gggcgcagcg tcctccacgc cgccactgcg agaaaggcgg cgagcaaagt tcccgcggcc
     5341 aggatgtaat cttcggtact gaggtgcagg atcagggccg gcacaccaag gagcccgaag
     5401 atgccggtgc tggcagcgaa ccccacgggt accagggtgg gccccgccat gtcgtcactg
     5461 aggagccagt gtgacaggag caaccctggc gccgtgaaga gtacgagggt ggcagcgaat
     5521 gcaacgggag agaaggctac tgagaggcct ctcaggggaa ggagcaggat cacagccgcc
     5581 gcgctaaaga gcaagacgag cgcatccgtc gatctcagac gagtcaagcc gctactccgg
     5641 ggcgacgagc taccgggaga ccgcgatttc acgaatcgcg tatgagcatg acgcgggcct
     5701 cagacgtctc catcggcgaa agctaacaca ctctcacccc tctcacccga gtattgcgac
     5761 acctggccgt tcttccgggt ggcgccgccc cggaggtgcg gtacaaatag cggatggaga
     5821 tcttcgagaa cctctcggca agttcgcggc tcgaggacgc ggagctcgac gcctactcgc
     5881 gggcggtgcg ggacttaaca gagagactgg agccggctgt gatctcggtc ggcctacccg
     5941 acggacgagg tggcggcagc ggggtgatac tcggcctcga aggcgactcg gccaccgcca
     6001 taaccaacag ccacgtcgta cgcggcctgc gtagccgcag ggctggcaac cctggcggag
     6061 acatacaggt cacgctctcg gacgggtcat cggtttcggc agagatgctc ggcgacgatc
     6121 cgccgagcga tctcgccgtc gtccgcttcg acaccgaaga agaaccgacg gtcgccacgc
     6181 tgggcgacgc gggggatcta gtagtcggcc agctcgtcgt cgcgatcggg aatcccctcg
     6241 gcttccagcg cagcgtcact gcgggggtgg tcagcgccct cgggcgttct atcaggggtg
     6301 agagcggccg gctcatagag aacgttatcc agaccgacgc ggcgatcaac ccaggcaact
     6361 ccggcgggcc gctggccgac gcctcagcaa gggtcgtcgg aatcaacacg gccatcatcg
     6421 gcggcgccca gggcataggc ttcgccgttc ccgtctcggg cgcgttccgc cgcgttgtct
     6481 ttacgctcgt aacggagggg cgggtgcgcc gcgcctacct cggggtggtg gtggagagcc
     6541 gccaggcgcg cggcgagttc gagggcggcg ccggagtgag gaacatagcc ccgaacagcc
     6601 cggccgagag ggccggcgtt aaaccgggag acgtcatcat cggcctgaaa ggggagccag
     6661 tccactcgaa cgacgatctc ctcaacctgc tcgacgcatc gtccatcggc caggacgcca
     6721 c
//
LOCUS       DASTKM010000313         5205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_129445, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000313 DASTKM010000000
VERSION     DASTKM010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129445"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VFQ10_12360"
     CDS             <1..721
                     /locus_tag="VFQ10_12360"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563035.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET9966180.1"
                     /translation="GETVTFPERDCSIQRRYQKLIEESPAPGLPGDVREGLMAASADL
                     IGSLGYEGAGTVEFVYADGEFYFIEMNTRLQVEHPVTEMISGVDIVAEQLKVVSGERL
                     EIPEDALSPDGHAIEFRINAEDPSRNFLPQAGLVEVYNPPGGPGVRVDSHLYAGYVVP
                     SHYDSLLAKLIVHARDREASIRRGIRALDEFAVSGMETTLPLHLAVLEDEEFRRGGVT
                     TQFLADRELESEGGLLRLTRT"
     gene            897..1718
                     /locus_tag="VFQ10_12365"
     CDS             897..1718
                     /locus_tag="VFQ10_12365"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET9966181.1"
                     /translation="MAKGVAMEKNEGRLVFGGMARWDGLDLFGPNYTSVAYRRKGEIH
                     IKIEPSVLRRPTNPAVQRVSRWPVIRSFFLWGRVFAQVFGSIWSLLFFAATLAILWLF
                     VSLMQLGSGAGTLGGVFALFAAFPILPILLIFFAAMKFTPIGRYHGAEHKAVAAYETY
                     GEVTLQNARNSSRIHPRCGTNILLYIILAALLDPLIAWWGYAILQFILISEAWFIFGQ
                     TRPSIAVGNFLQRYLTTTEPRRYQLEVAVESLNRLLQAEEEREIDEAAVRVKARY"
     gene            complement(1750..2706)
                     /locus_tag="VFQ10_12370"
     CDS             complement(1750..2706)
                     /locus_tag="VFQ10_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966182.1"
                     /translation="MNYGDLIRDAYRITLHNRYLWFFGFFAGGTGTNFVGNVPSGGGN
                     FNFDDFQRSGSDISGLTAQVGQGLSETVVIFGIVLVVLLIALFFIILAIISQGALADS
                     VAAIDRGEGRRFGSAFTSGMGNFWRVLGYYVVFFLIALGLLMVIAIPVALLIGGTFAA
                     TQSTGVRVSVAVVVGILAVLLLIVVFIPLSIIGQYALRDIVVRRERVLGSVGSGYNLF
                     RHNIGRSLLLWLIQLGISIGIGIAFILMLLIVGLILALPAIALAVAGYTTAAIIAGVI
                     AGLILLPFLLVATGAIGTFSHAYWTLAYLRLTAPPTPSPTPG"
     gene            complement(2703..4103)
                     /locus_tag="VFQ10_12375"
     CDS             complement(2703..4103)
                     /locus_tag="VFQ10_12375"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET9966183.1"
                     /translation="MRFERALPGPNEVFLPEGTLRRIYEPVFDELGRTGAERWEGKLR
                     EAHRLLLDEQHAFGIKEGDKTHPTDWIPRIVPASVWERLEAGLTQRLRAINLFLSRLE
                     AGKEEVVPREVVESSILYDTGWKDRFGPVPVRQIAFDVVAVEGRQGWEYLVIEENAKM
                     PVGLAAMTRRRRMSGEVFFPGSMSKLPVLPLDGWLGRLGDALRAASPRGPDATLAVVS
                     SGPDDQFYLDHHIYAKEMDAILAETKDLEVDGDGYLIYKPTGRRVDVIYERVDEDTLY
                     AEVPGLLESHIEGKVHVLFAPNSEIIDDKGVGAFVPEMVRRYLGEEPLIQNARTWSLA
                     VEEDRRYVMDHFDELVVKSRGGYGGKEVMIGPEESRESLQKFRRQVEATPVEYVAQET
                     IDFSTHVLCETEGADFLLRDSYADYRVLVLSPDPADPYVVEAVPGSLSRVAAPGKHVV
                     NISSGGKMKDTWVLES"
     gene            4252..4482
                     /locus_tag="VFQ10_12380"
     CDS             4252..4482
                     /locus_tag="VFQ10_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966184.1"
                     /translation="MLLGGWISCAIGVLLAVGTVFAAFLGGGPNISASALGVGFGVLG
                     YFLGARKPATATVFLCAAAILFGLAAGQGYIG"
     gene            4603..>5205
                     /locus_tag="VFQ10_12385"
     CDS             4603..>5205
                     /locus_tag="VFQ10_12385"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET9966185.1"
                     /translation="MERVRLTPPGEKRARGGRARLYVRWMPHLGAEAVALYELLRILP
                     DLGNESPEIEELAELLRSTPEGVESSLEALIEARFVVSREGWLEVAEHPPSEETNERP
                     REDDLPDRDVEVIRAEPDGVSADDYFRFMGSLPAPHILEFMNGYCGRDGLSPDAVREA
                     LKIASERDARRVGYVRSILERWVERGVKTVEDVERFEQDRK"
BASE COUNT          977 a         1616 c         1653 g          959 t
ORIGIN      
        1 cggtgagacg gtaaccttcc ccgagaggga ctgctccatc cagcgccgct accagaagct
       61 tatcgaggaa tcgcctgcgc cgggcctgcc aggggacgtg agagagggtc tcatggcggc
      121 ctcggccgat ctgatcggct ccctgggcta cgagggtgct gggaccgtgg agttcgtcta
      181 cgcggacgga gaattctact ttatcgagat gaacacccgc ctccaggtgg agcatcctgt
      241 gacggagatg atctccggcg tcgacatcgt tgccgaacag ctgaaggtgg tctcgggcga
      301 gcgtctggag atccctgagg atgccctctc tcccgacggg cacgcgatag agttcaggat
      361 caacgccgag gaccccagcc gcaacttcct gccccaggcc ggtcttgtcg aggtctataa
      421 tccgcccggc gggccgggcg tcagggtcga ctcccacctc tacgccggct acgtggtgcc
      481 ctcgcactac gactccctcc ttgcgaagct catcgtgcac gctcgtgacc gcgaggcctc
      541 gattcgccgg ggaattcggg ccctcgacga gttcgccgtc tctggcatgg agacgaccct
      601 cccgctgcac ctcgccgtgc tcgaggacga ggagttcagg aggggcggcg tcaccacgca
      661 gttcctggcg gatagggaac tcgaaagcga gggcggcctc ctgcggctaa ccaggacatg
      721 agcggactgc aaataggaat acaagcactt cagccagtca gctctttgct gaagtgctca
      781 caagcggaag ccaatggctg gagcgtgccg acacgggcgg gtcggagaag ggcaccttcc
      841 ccgacccgcc cgtgttaatc tttattgcct gatccttgcc tagaagaggg agaactgtgg
      901 ccaaaggcgt cgcgatggag aaaaacgagg ggcgtctagt cttcggtggg atggcccggt
      961 gggacgggct cgacctcttc ggcccgaact ataccagcgt cgcgtatcgc aggaaggggg
     1021 agatccacat caagatcgaa ccctccgtgt tgcgaaggcc cacaaacccc gccgtacaac
     1081 gcgtctcccg ctggcctgta atacgttcgt tctttctgtg gggcagggtc ttcgcccagg
     1141 tcttcggatc tatctggagt ctgctcttct tcgcggccac gctggccatc ctctggctct
     1201 ttgtgagcct gatgcaactc ggtagcgggg cgggaaccct tggcggcgtc ttcgccttgt
     1261 tcgcggcgtt ccccatccta cctatcctgc tgatcttctt cgcggctatg aagttcaccc
     1321 ccatcggccg ctaccacggg gccgaacaca aagccgtcgc cgcctacgag acgtatggtg
     1381 aggtgacgct ccagaacgcc agaaacagca gccgtatcca cccccgctgc gggacgaaca
     1441 tcctgctcta cataatcctc gcagccctcc tcgacccgct gatagcgtgg tggggctacg
     1501 ctatcctcca gttcatcctc atctccgaag cctggttcat cttcggccag acccgaccct
     1561 cgatagcagt aggcaacttc ctgcagagat acctcaccac caccgagccc cgccgctacc
     1621 agctggaggt cgcggtcgag tccctgaaca ggctgctcca ggccgaagaa gagcgtgaga
     1681 tcgacgaggc ggccgtacgg gtgaaggcga gatattgagg cagcatgtca gcttgtctgc
     1741 cggcctgact tatccggggg tgggggatgg ggttggcggg gccgtcaatc gcaggtaggc
     1801 gagcgtccag taggcgtggg agaaggtgcc tatggcgccc gtcgccacga gcaggaacgg
     1861 gagcaggatc aggcccgcta tcacgccggc gatgatggct gctgtggtgt acccggcgac
     1921 ggccagcgcg atcgccggta gggccaggat gaggccgacg atcagtaaca tcaggataaa
     1981 cgcgatcccg atccctatag agatcccaag ctgtatgagc cagagcaaca ggctccttcc
     2041 gatgttgtgg cggaagagat tgtagccgct cccgacggag ccgaggacgc gctccctgcg
     2101 caccacgatg tcccgcaggg cgtactggcc gattatggag agggggataa agacgacgat
     2161 gagcaacagg acggccagaa tccccactac caccgcgacg gagacccgca cgcccgtgga
     2221 ctgggtggcc gcgaacgtac ccccgatcaa aagcgcaacg gggatagcaa tcaccatgag
     2281 gagcccaagc gcgatcagga agaacacgac gtagtagccg agcacgcgcc agaaattacc
     2341 catcccggac gtgaaggcgg acccgaaccg gcgtccctcg cccctgtcta tggcggcgac
     2401 gctatcggcc agcgccccct gggagattat cgccaggatg atgaagaaca gcgcgatcag
     2461 tagcaccacc aagacgatac cgaagatcac gaccgtctcg ctcagtcctt gcccgacctg
     2521 ggctgtgagc ccggagatgt ctgagcccga gcgctggaag tcatcgaagt tgaagttacc
     2581 acccccggag gggacattgc ccacgaagtt ggtgcccgtg ccgccggcga agaagccgaa
     2641 gaaccacagg tagcggttgt ggagcgtgat cctgtacgcg tcccgaatga ggtctccgta
     2701 gttcatgatt cgagcaccca ggtatctttc atcttgcctc cactcgagat gttgacgacg
     2761 tgcttgcccg gagcggcgac ccggctcagc gaacctggga cagcctcgac cacgtacggg
     2821 tctgcagggt cgggagagag gacgagcacg cggtagtccg cataggagtc gcgaaggagg
     2881 aaatctgccc cctctgtctc gcagaggacg tgggtggaga agtctatcgt ctcctgggcc
     2941 acgtactcga caggggttgc ttcgacctgc ctgcggaact tctgaagcga ttctcgcgac
     3001 tcttctggtc cgatcatgac ctccttgcct ccgtagcccc ccctgctctt tacgaccagt
     3061 tcatcgaagt ggtccatgac gtagcgcctg tcctcctcta cggccagcga ccaggtcctg
     3121 gcgttctgta tgaggggttc ctctccgagg tagcggcgca ccatctcggg gacgaaggcg
     3181 ccgacgccct tgtcgtctat tatctctgaa ttgggggcga agagcacgtg tactttgccc
     3241 tcgatgtgag actcgagcag accaggcacc tcagcataga gcgtatcctc gtcgacgcgc
     3301 tcgtagatca cgtctaccct gcgtcctgta ggcttgtaga tgaggtatcc atccccgtct
     3361 acttccagat cctttgtttc ggcgaggatg gcgtccatct ccttggcgta gatgtggtgg
     3421 tcgaggtaga actggtcgtc tggaccactg cttacgacgg ccagcgttgc atctgggcct
     3481 ctgggggagg cggcccgtaa agcgtcgccg aggcgtccga gccagccgtc gagaggcagg
     3541 acgggtagct tggacataga accggggaag aagacctccc cgctcatcct gcgtctgcgg
     3601 gtcatcgcgg cgaggccgac tggcatcttg gcgttctcct cgatgaccag gtactcccag
     3661 ccttgcctgc cctctacggc gacgacatcg aatgctatct ggcgcacggg cacgggaccg
     3721 aaacggtcct tccaacccgt gtcgtatagg atgctgctct cgacgacctc ccttggaacg
     3781 acttcctcct tgccggcttc gaggcggctc aggaacaagt tgatggcgcg caggcgctgg
     3841 gtcaggccgg cctcgagacg ttcccagacc gaggccggta cgatgcgggg gatccagtcc
     3901 gtaggatggg tcttgtcgcc ctcctttatg ccgaacgcgt gctgctcgtc gagcagtagc
     3961 ctgtgcgcct cgcgcagctt cccttcccac ctctcggcgc ctgtgcgtcc cagctcgtcg
     4021 aagaccggct cgtagatccg gcgcagagtt ccttcgggga ggaagacctc gtttgggcca
     4081 gggagtgcgc gctcgaacct cacaggttgg ggattatact agtcccccaa ccgggtacgc
     4141 tctgtcgtgc caatagacca cggccaaggg gagcggattc tgggcgaaaa cacggacaca
     4201 cggatacctg aagacgaagc gggcaatgat ccagtacaag agggacgcgc gatgctgctc
     4261 ggcggctgga tctcctgcgc catcggcgta cttctcgcag taggcacggt cttcgccgcg
     4321 ttccttggcg ggggcccaaa catctccgcc agcgcgcttg gtgtgggctt cggtgtcctc
     4381 ggctacttcc tcggcgccag gaaacccgcg accgcgacgg tcttcctctg cgccgccgct
     4441 atcctcttcg ggctcgccgc gggccagggc tatattggct agtcagcacg gcggctgcgc
     4501 cgccttttca gcatgtcagc aagggctcgc acacgcttca agctgaaatg ctgacaatca
     4561 gatgtagaga gcatagcccc tggccgctat actggcttca ggatggagag agttcggctc
     4621 acacctccgg gggagaagag ggcgcgggga ggccgcgccc gtctctacgt ccgctggatg
     4681 ccgcacctgg gggccgaggc agtcgcgctc tacgagctgc tccgcatcct ccccgacctc
     4741 gggaacgaat ctcctgagat cgaagagctc gcagagctgt tgcggtccac gcctgagggc
     4801 gtcgagtcat ctctggaggc cctgatcgaa gcccgcttcg tcgtcagtag ggagggctgg
     4861 ctcgaggtcg ccgagcaccc gccttcggaa gaaacgaacg agcggccccg cgaagacgac
     4921 cttcccgacc gcgacgtgga ggtgataagg gccgagccag acggggtctc ggccgacgat
     4981 tacttccgtt ttatggggtc attgccagcg cctcacatcc tggagttcat gaacggttac
     5041 tgtgggcgtg atgggctatc gcctgacgcg gtgagggagg ctttgaagat agccagcgag
     5101 cgcgacgcgc gcagggtcgg ctacgtgcgg agcatcctgg agcggtgggt cgagcggggc
     5161 gtgaagaccg tcgaggacgt ggagcgcttc gagcaggacc gcaag
//
LOCUS       DASTKM010000314         6117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_311029, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000314 DASTKM010000000
VERSION     DASTKM010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311029"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VFQ10_12390"
     CDS             <1..1063
                     /locus_tag="VFQ10_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET9966186.1"
                     /translation="RMICDVRTPDGDPYVGDPRHVLRRALERASEMGFDKFYCGPELE
                     YFYFRDSKGTEPLDYGGYFDLTTLDAATALRRETVQSLQQLGIKVEYSHHEVGISQHE
                     IDLRFDDAMAMADKVMTYKTVVKEVATRHGVYATFMPKPLRDQNGSGMHTHQSLFSGG
                     KNAFFDADNEYYLSKEAQAFIAGQLRHAREISIIFAQHVNSYKRLVPGYEAPVYIAWS
                     RRNRSALVRVPLYHPGKERATRMELRCPDPAANIYLCFAVLLHAGLEGIEKGYELPEP
                     MERNLYHLTAEEREKMGIESLPADLGEAIKEAENSELLHKALGEHTYTRLLQLKREEF
                     EDYRVQVTPYELEKYLPVL"
     gene            complement(1119..1889)
                     /locus_tag="VFQ10_12395"
     CDS             complement(1119..1889)
                     /locus_tag="VFQ10_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9966187.1"
                     /translation="MNLRPYLEFGVQIAYEAGRLTLGYFGTEAARPEFKADDTPVTLA
                     DREAERLIRERIGARYPKHAVLGEEYDEVEGADTDHRWVVDPIDGTKSFVRGVPLYGV
                     LIGLEIEGVCEVGAAYFPAIDEMVCAATGEGCYRNGRRARVSTTQSLAQGIVSFTDAD
                     SFEEHGRWEAWLRVLSAAGGSRGWSDAYGHALVATGRVELMLDPIMNTWDCGPFPPIL
                     REAGGYFGDWCGNETIYSGEAMSTPRSLLPEVLSLIEG"
     gene            2035..3489
                     /locus_tag="VFQ10_12400"
     CDS             2035..3489
                     /locus_tag="VFQ10_12400"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET9966188.1"
                     /translation="MVTIGATSRASEEFAPELLAVALYKGESPRDLGEEAAWVVSDGD
                     FGGKTGETALLYGQDGPAPRLLLVGLGERELFTLEKLRRAAATVARRARTLKLHSAAF
                     SLPTLPDTAIREIARTVAEGARLGLYRFDRHKSAADDHDLQTFWLVADEGVLDEATEG
                     AELGTKVAEGTVLARDLANEPSNVATPEYLAERARAIAEKYGMVLDVLDRAGIEEEGL
                     TGLATVGRSSSNEPRFIVLEHRGGGDSAPVVLVGKAVTFDSGGISIKPSAGMEDMKFD
                     MSGGSAVLGAMEAAGALDLPLNVVVLVPATENLPGGDAFKPGDVLQMHSGKTVEIVTT
                     DAEGRLILADALSYATRYEPSAVVDCATLTGACAVALGDHASGLMGNDENLVAELQTA
                     GETTGERTWPLPLFEEYTEQIKGDAADIKNSGGRRGGALTAGAFLKEFADYPWAHLDI
                     AGTAYGKKGNAYTTKGATGVPARLLVTFLLGRSQ"
     gene            3486..4124
                     /locus_tag="VFQ10_12405"
     CDS             3486..4124
                     /locus_tag="VFQ10_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9966189.1"
                     /translation="MILEMLTVGPFQENCYIIGDEDSGVGAVVDPGDEAARIALAVEQ
                     TNLEVGSIIVTHAHIDHVGAVAALVDEYTCPVLMHAESEPMLKQLPTQAIMMGLKFGK
                     VPAVDRHIGDGDVLEVGELRLRSLYTPGHAPGHLAFYVEDQGLVLSGDALFAGSVGRV
                     DLPGGSMEVLMRSIEDRLLTLPDETTVYPGHGPQTTIGNERTSNPFLQGGLF"
     gene            4121..4645
                     /locus_tag="VFQ10_12410"
     CDS             4121..4645
                     /locus_tag="VFQ10_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET9966190.1"
                     /translation="MSESVHRHRESAPETVRVAILTISDTRTRESDTGGDTAEHLLRG
                     AGQEIVARKIVRDEVTGIRANLIDLLANPDVDAVITTGGTGISGRDTTYEVVDRMIEK
                     KLDGFGEIFRMLSYEEIGAAAIMSRAVAGAVGTKFIASLPGSRNAVKLAAEKLLVPEL
                     AHIVFELRKHEESG"
     gene            4650..4907
                     /locus_tag="VFQ10_12415"
     CDS             4650..4907
                     /locus_tag="VFQ10_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966191.1"
                     /translation="MEREPLSSEADALWRRLWEIWQDNDEEDVVLDSTELAELEEEIP
                     GLEKRMKTALAYLQRARYIQYRSGVGEDGIEPILYDVYEPR"
     gene            4937..5383
                     /locus_tag="VFQ10_12420"
     CDS             4937..5383
                     /locus_tag="VFQ10_12420"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9966192.1"
                     /translation="MTWLLLLPVALAAGMVAPTQFAINSQLRQVVGGPVLAAALSFLV
                     GTVILFATTAVVRRSVPELGPIMNAPWWMWLGGLLGAFFVCASIVLTPRLGAATTVGL
                     FLAGQVIASIAIDHFGLLGVTAQPASIPRLLGALLIIVGVAMVQRF"
     gene            complement(5563..>6117)
                     /locus_tag="VFQ10_12425"
     CDS             complement(5563..>6117)
                     /locus_tag="VFQ10_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9966193.1"
                     /translation="IEHLELGAADVMGYSLGGGVALRTAIQHPEVVRKLVLVSTPFRR
                     DGWYPEVLAGMDQMGPAAAEAMKQTPMYQLYSSVAPRPEDWPVLLTKLGGLLGQDYDW
                     SQEAAAMEVPALIVVGDADSVRTSHAVAFFELLGGGKQDAGWDGSGMSDARLAILPDT
                     THYGIFSSPALVAAVTPFLDTPEP"
BASE COUNT         1164 a         1940 c         1930 g         1083 t
ORIGIN      
        1 gcgtatgatc tgcgacgtca ggacgcccga tggtgatccc tacgtcggtg acccgcgcca
       61 cgtcctgagg cgtgccctgg agcgggcgag cgagatgggc ttcgacaaat tctactgcgg
      121 acccgagcta gagtacttct acttcaggga ctccaaaggg acagagcctc tcgactacgg
      181 cggctacttc gacctgacga cgctcgacgc ggcgacggcc ttgaggcgcg agacggtaca
      241 gtctctgcag cagctcggca taaaggtcga gtactcccac cacgaggtcg gcatctccca
      301 gcacgagatc gacctccgct tcgacgacgc tatggcgatg gcagacaagg tgatgaccta
      361 caagaccgtg gtcaaagagg tcgccacccg ccacggcgtc tacgccacgt tcatgcccaa
      421 gcccttgagg gaccagaacg gctcggggat gcacacccac cagagcctgt tctcgggcgg
      481 caagaacgcc ttcttcgacg cggacaacga gtactacctc tccaaggagg cgcaggcttt
      541 catcgccggc cagctccgtc acgcgcgtga gatcagcata atcttcgccc agcacgtgaa
      601 ctcttacaag cgtctggtcc ccggttatga agcccctgtc tacatcgcct ggagccgccg
      661 caaccgctcc gcgctggtca gggtcccgct ctaccaccca ggcaaggaga gggccacgcg
      721 gatggagctc cgctgccccg acccagcagc caacatctac ctctgctttg cggtgctctt
      781 gcacgccggc ctggaaggca tcgagaaggg ctacgagctg cctgaaccga tggagcgcaa
      841 cctttaccat ttgacggccg aggagcgcga gaagatggga attgagagcc ttccagcgga
      901 cctcggagag gcgatcaagg aggcggagaa ctcggagctg ctgcacaagg cgctcggaga
      961 gcacacctac acccgcctcc tgcaactcaa gcgcgaggag ttcgaagact acagggtgca
     1021 ggtaactccc tacgagctcg aaaagtatct gcctgtcttg taggtgtcag gttgggtccg
     1081 gctcacgccg gacccctcgt gctccagccc ggctttatct aaccttcgat gaggctcaga
     1141 acctcaggaa ggagtgacct tggggtgctc atcgcttcgc ccgagtagat cgtctcgttg
     1201 ccgcaccagt cgccgaagta ccccccggcc tcgcgaagga tgggtgggaa agggccgcag
     1261 tcccaggtgt tcatgatggg atcgagcatc agctcgactc tccccgtggc gacgagggcg
     1321 tggccgtaag cgtcggacca gccgcggctg cctcctgcgg ccgagagaac acgcagccag
     1381 gcctcccagc ggccgtgctc ctcgaagctg tccgcgtcgg tgaaagaaac tatgccttgc
     1441 gccaggctct gtgtggttga cactcgcgcc ctgcggccgt ttctgtagca tccttcgccg
     1501 gtggcggcgc acaccatctc gtcgatggca ggaaaatagg cggcgccgac ctcgcagacg
     1561 ccttctatct ccaggccgat caggacgccg tacagaggga cgccgcggac gaaagacttt
     1621 gtgccgtcta tggggtccac gacccagcgg tggtcagtgt cggccccctc cacctcatcg
     1681 tactcttctc ccagtaccgc gtgcttcgga tagcgagccc ctatcctctc acggataagc
     1741 ctctcggcct cgcggtcggc cagtgttaca ggcgtatcgt cggccttgaa ctcgggacgc
     1801 gccgcctccg ttccgaagta gccgagcgtg agccttccgg cctcgtaagc gatctggacg
     1861 ccaaactcga ggtaagggcg caggttcatg accccaagat agcatccttc ctgtgcccga
     1921 gatgccggaa cagccccgtt cgcgtgggtc cactgggccc gcagattaga ctgcctctgc
     1981 ctttccccta taatctatgc ggtcctgtgt cttgcgtaga agggtggtct ggacttggtc
     2041 acgatcggcg ctacgagccg agcaagcgag gaattcgcgc cagagctact cgcggttgcg
     2101 ctatataaag gggagagccc gcgggatctc ggcgaagaag ccgcctgggt cgtgtcagac
     2161 ggggatttcg gcggcaagac gggcgagacg gccctgctct acgggcagga tgggcctgcg
     2221 ccccgcctct tgctcgtcgg cctcggcgag agggaactct tcaccctgga gaagctgcgc
     2281 cgcgcagcgg ccaccgttgc cagacgcgcc aggaccctca agctgcacag cgcggcattc
     2341 tctctgccta ccctccctga cacggcgata agggagatcg cgcgaacagt cgccgaaggg
     2401 gccaggctgg gcctgtaccg cttcgacagg cacaagagtg cagcggacga ccacgatctc
     2461 caaacgttct ggctggtcgc cgacgaaggg gtgcttgacg aggccacaga gggtgccgaa
     2521 ctgggtacca aggtcgctga ggggaccgtg ctggcccgcg acctcgccaa cgagccgtcc
     2581 aacgtggcta ctccggagta tctcgcagag agggcccgcg cgatcgctga aaagtacggt
     2641 atggtgctcg acgttctcga ccgggccggt atcgaagaag agggactcac gggtctcgcg
     2701 acggtgggcc gctcatcctc caacgagccg cggttcatcg tgctcgagca ccgcggggga
     2761 ggcgacagtg cacccgtcgt cctcgtgggg aaggctgtaa ccttcgactc gggtggtatc
     2821 tccataaagc catcggccgg aatggaggac atgaagttcg acatgagcgg cggcagcgcc
     2881 gtcctcgggg cgatggaggc cgcgggggct ctggacctcc cgctcaacgt cgtcgtcctc
     2941 gtgccggcga ccgagaacct gcctggcggc gacgctttca agcccggcga cgtactccag
     3001 atgcactccg ggaagactgt agagatagtt acgaccgacg cggaggggcg tttgatcctc
     3061 gctgacgccc tctcttacgc cacgcgctac gagccatccg ccgtggttga ctgcgccacc
     3121 ctgacgggag cctgcgccgt cgcgctcgga gaccacgcct caggcctcat ggggaacgac
     3181 gagaacctcg ttgcggagct acagaccgcg ggtgagacga ccggggagag gacctggccg
     3241 ctgccgctct tcgaggagta cacagagcag atcaaaggcg acgccgcaga cataaagaac
     3301 tccggcggcc gcagaggagg ggccttgacc gccggtgctt tcctaaagga gtttgccgac
     3361 tacccgtggg cgcacctgga tatagctgga accgcctacg gcaagaaggg caacgcctac
     3421 acgacaaagg gcgcgacagg cgtacctgcc cgcctgcttg tcacgtttct tctcggaagg
     3481 tcgcaatgat cctggagatg ctcaccgtcg gcccattcca ggagaactgc tacatcatcg
     3541 gcgatgagga ctctggggtt ggcgcggtcg tcgatcccgg cgatgaggcg gcccggatcg
     3601 cgctcgcggt cgagcagacg aacctggagg ttggatccat aatcgttacg cacgcccaca
     3661 tagaccacgt cggggccgtc gccgccctcg tcgacgagta cacctgcccg gtcctgatgc
     3721 acgccgagtc cgaacccatg ctgaagcagc ttcctacaca ggcgattatg atgggcctga
     3781 agttcggcaa ggtccctgct gtggaccgcc acatcggcga tggagatgtg ctcgaggtcg
     3841 gagaactcag gctgcgctcg ctctacacac caggccacgc gcctggacac ctggctttct
     3901 acgtcgagga ccagggtctc gttctgtcag gcgacgccct cttcgcggga agcgtgggaa
     3961 gggtagatct ccctggcggg agcatggagg tactgatgcg cagcatcgag gatcggctgt
     4021 tgacgctgcc tgacgagacg acggtctatc ccggtcacgg gccgcagacc actatcggga
     4081 acgagcgcac gtccaaccca tttctgcagg gagggctgtt ttgagcgaga gtgttcacag
     4141 gcacagggaa tcggccccgg agacggtcag ggtcgccata ctcaccatca gcgacacgag
     4201 gacccgcgag agcgacacgg gaggcgacac cgcagaacat ctgttgcgcg gagcagggca
     4261 ggagatcgtg gcccgcaaga tcgtcaggga cgaggtcacc ggcatcaggg cgaacctgat
     4321 agacctcctt gcgaaccccg acgtggacgc tgttattacg acgggaggga cggggatctc
     4381 agggcgcgac accacctacg aggtcgtcga ccgcatgatc gagaagaagc tcgacggttt
     4441 cggtgagata ttcaggatgc tctcgtacga ggagatcggg gccgcggcga tcatgagccg
     4501 cgcggtcgct ggagccgtgg gcaccaagtt tatagcgagc ctgccaggat ctcgcaacgc
     4561 ggtgaagctc gctgcggaga aactcctcgt cccggagctg gcccacatcg tgttcgagct
     4621 tcgcaagcac gaggaaagcg ggtagagaga tggaaagaga acccctatca tccgaagccg
     4681 atgcgctctg gcgcaggctg tgggagatct ggcaggacaa cgatgaggag gacgtggtcc
     4741 tcgattccac cgagctggcc gagctcgaag aggagattcc cggcctcgaa aaacggatga
     4801 agacggccct cgcctacctc cagcgcgccc gttacatcca gtaccgctcc ggggtcggtg
     4861 aggatggcat agagccgatt ctctacgacg tctatgagcc gcgctgagcc catgaaaacc
     4921 tagtggagtg aggagtatga cctggctact cttgctaccc gtggcgctcg ccgctgggat
     4981 ggtcgcccca acgcagttcg ccatcaacag ccagctgcgc caggtcgtcg gcggtcctgt
     5041 tctggcagcc gcgctctcgt ttctcgttgg gaccgtgatc ctcttcgcga ccaccgctgt
     5101 cgtgcgtcgc tccgtgcccg agcttgggcc catcatgaac gcgccctggt ggatgtggct
     5161 cggcggtctg ctcggggctt tcttcgtgtg cgcctcgatc gtcctgaccc cccgcctggg
     5221 ggccgcgacg acggtaggcc tgttcctcgc ggggcaagtg atcgcgtcca tagcgataga
     5281 ccatttcggc ctgctcggcg tcaccgcgca accagcgagc ataccccgcc ttctgggggc
     5341 cctgctcatc atagttgggg tcgctatggt ccagaggttc tgagtttgca aagaggttca
     5401 aaacctcatt gcaagcttgt cagcttctag gtttcgctca ttgccgaatg tgcgattcgc
     5461 ggtagatcga tacttctacg gctcgcttta cactttgtag atcactggtt tcggccggta
     5521 ccgaccccgg tcatcgttcc tgcgattctc gccccgttat ccttatggct ctggcgtgtc
     5581 caggaacggc gtgacggcgg caaccagagc tggagaagag aagatgccgt aatgcgtcgt
     5641 gtctggcaag atggcgagtc tggcatcgga cattccggaa ccatcccacc cggcatcttg
     5701 cttaccgccg ccgagcaact cgaagaacgc caccgcgtgc gatgtgcgga cactatcggc
     5761 atccccgaca acgatcaacg caggcacctc catcgccgcc gcttcctgag accagtcgta
     5821 gtcctgacct agaagtccgc ccagcttggt gagcaagacg ggccagtctt ctggcctcgg
     5881 cgcgacgctg gagtagagtt ggtacatcgg cgtctgcttc atcgcctccg cggcagcggg
     5941 gcccatctgg tccatccccg cgaggacctc ggggtaccat ccatcgcgcc tgaacggggt
     6001 cgagacgagc acgagcttcc tcaccacctc tggatgctgg atcgccgttc gcaaggcgac
     6061 cccgccgccg agagagtagc ccatgacgtc ggccgccccc aactccaggt gttcgat
//
LOCUS       DASTKM010000315        13558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_839779, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000315 DASTKM010000000
VERSION     DASTKM010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 13558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13558
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839779"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..421
                     /locus_tag="VFQ10_12430"
     CDS             128..421
                     /locus_tag="VFQ10_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966194.1"
                     /translation="MFDAAIGAADASADYDKARTCAVQKLEHLKSIPYEAVEGGPKAG
                     VCNPSGFAYTIATQSIGTDLGDSSGETGLMMVTVTVDWDGENSYVVSGVVSRW"
     gene            415..1050
                     /locus_tag="VFQ10_12435"
     CDS             415..1050
                     /locus_tag="VFQ10_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966195.1"
                     /translation="MVNLARSRLWKEETGFTLVEVLVSAMMMFGVLFALYAIFDVSVR
                     AFGYGGDRLEAVGNARLALGRMEREIRAAYPYDLTSDPPRRYLFLDPTDPTGPAVPAA
                     TRVTFGNETGGDRRIEGSEVIGYYLSGHDLKRSKGGSAQTLVDSVPADGLRFTPCRSA
                     DDCPPALAITDEAEIRLLRIELAVEVRRRGQDPARQTLATDVYLRNRADQR"
     gene            1047..1466
                     /locus_tag="VFQ10_12440"
     CDS             1047..1466
                     /locus_tag="VFQ10_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966196.1"
                     /translation="MIRRLLREERGMALGLAVIVVVIVGVMGAGLLTFVATDLEAVVA
                     VNRGEQAFELAEAGIEVAKAHLANDASQADWSSGELHMASMVEGSVTVTVEYDADSNS
                     FEAVSIGQYRETNRKLEATFSVEDGAPKLLSWRELYE"
     gene            1529..1786
                     /locus_tag="VFQ10_12445"
     CDS             1529..1786
                     /locus_tag="VFQ10_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966197.1"
                     /translation="MDEKTRTRVGGPDDPREGLEAVVALRRTLEALEAAQVENAIVAG
                     WSWARIAEVLGVSKQAVHKKHARRIRQKYPGEPPRRKGRSL"
     gene            1783..2292
                     /locus_tag="VFQ10_12450"
     CDS             1783..2292
                     /locus_tag="VFQ10_12450"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET9966198.1"
                     /translation="MIFTRFSDRSRMCVEAALDEARMLGHDSLGDEDLLLGILRADDG
                     IGAEALSSLGVTLEGAREESEEMLSGALASIGISLEEVRREAGDAFELRIPDKRRITI
                     SPWAKKALVEARKEMRRLGDNHLGTEHVLLGILRNADGTAVRMLSGMGVSPEVVEERL
                     FELRGRAAS"
     gene            2463..2897
                     /locus_tag="VFQ10_12455"
     CDS             2463..2897
                     /locus_tag="VFQ10_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966199.1"
                     /translation="MEEREERGGAVRVGMIWGGIGGVVGLLVSLLGSLVGILVAGFIG
                     FSCGRRAAVAGSRAGALSGLVGGAVAAPVYVLGSTLGALLAARQIGAAGIATTLSDVL
                     GTRVSADEAWIYFLFSLILSAILEAVVLILVSSAAGAWAKRE"
     gene            complement(2923..3399)
                     /locus_tag="VFQ10_12460"
     CDS             complement(2923..3399)
                     /locus_tag="VFQ10_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET9966200.1"
                     /translation="MDRQLQIQILLDHYEKPRHRGAMEDADVAMPGGNPGCGDVVTIY
                     LKAADDQEHIERATYEGEGCTISMAASSMLLEDVQRSDLTMDQVLELDYHEMIEQLGR
                     QVVASRPKCATLGLGTLKAAIRKYQKDRRLEKAGVTRPDVGEFVFGEHAAEAARER"
     gene            3503..4585
                     /gene="ychF"
                     /locus_tag="VFQ10_12465"
     CDS             3503..4585
                     /gene="ychF"
                     /locus_tag="VFQ10_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET9966201.1"
                     /translation="MKVGIVGLPNVGKSTVFNSLTRAGAEAQNYPFTTIDPNLGVATI
                     QDERLVAVAGVIGSKRVVPATVDFVDIAGLVGGASAGEGLGNQFLGHIRECDAVAHVV
                     RCFEDENVVHVHAGVDPRGDIETIDTELLLADLASVERRLERVGRAAKGGDARLEDEA
                     AELERLRDHLSDGSPARAYPATETLAEAMSTLITAKPTLYVANVDEESVAEGNRYSSQ
                     VEKHSSSEGAEAVRICAKLEAEIAELPEDEAAEYMAMLGVETTGFEEFVRAAYRLLGL
                     VTFFTAAEKEGRAWTIRQGSTVRQAAGRIHTDMERGFIGAEVGDWQDIVAAGSWARAK
                     EEAKVRLEGRDYVVRDGDTVLVRFNV"
     gene            4597..4992
                     /locus_tag="VFQ10_12470"
     CDS             4597..4992
                     /locus_tag="VFQ10_12470"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HET9966202.1"
                     /translation="MQPFVKPKKSLDRSRLALVTTGGVHVPDQPRFDIDDPAGDCSYR
                     EIPATANDLTWTHAYYRPDEGSDLDAVFPLETLCRLVRDGEVGELAPRHFSFMGAIHD
                     PAPLVESTAPEVAGKLVEDQVDVVLLTPS"
     gene            5428..6126
                     /locus_tag="VFQ10_12475"
     CDS             5428..6126
                     /locus_tag="VFQ10_12475"
                     /inference="COORDINATES: protein motif:HMM:NF039426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HET9966203.1"
                     /translation="MALLAVLFIAFFVIAFLFLLPILFSLQAVGNLFVYPRQLRAIFG
                     NKILRRNHALEHATVAVMMEREPGRKLNGFSTDDGFFVQGVRSISEVESAAREALRRL
                     QNGEKRLAIHRNCGTTIVAANLLAAILFLVALVLGVLYLGVGGDLYLMILGSVVLAFA
                     LRVPLSLLLQRFVTTDADLENAEVGWVEPAQPGDLKSGIFGLLLAASTARVRVFHTDP
                     DAVEILRDDGAIVR"
     gene            complement(6201..7028)
                     /locus_tag="VFQ10_12480"
     CDS             complement(6201..7028)
                     /locus_tag="VFQ10_12480"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HET9966204.1"
                     /translation="MRIYGLDFTSAPGHRKPLIVLGCTLEGGSLRVEDSETLTDFGGF
                     EDFLQRRGPWVCGMDFPFGQPRSLVAALGWPESWEGYVGEVGSLPKEEFEDKIRADMA
                     KRPAGSKWRYRLADRRSGSSSAMMLFRVPVGKMFYQGAPRLLASGVRVEPCRRNGDER
                     VAVEAYPAVVARRFLGHTAYKRDAAPDTPERRSARETLLRGLESTALSEVYGFAVEMD
                     ARWRDEFVRDPAGDALDSLLCAVQAAWAYEKRDEGYGVPPECDPDEGWILDPALLDE"
     gene            complement(7050..8045)
                     /locus_tag="VFQ10_12485"
     CDS             complement(7050..8045)
                     /locus_tag="VFQ10_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9966205.1"
                     /translation="MKIPGMRLIKKAKRKGNEVEVPVAENLGLVDFSKLTYKEMNEDH
                     VMAFAGNLTYKGLFAIFPFFTFLLSLLGVFNATDLVNVMVDKLSGIAPQSATRFIEGQ
                     LLSITQSQAESAFTFGAIISILLALWGISGAFRSVMEAMNVMYEVEEDRPFWKVYGLS
                     IFMSLAVVVLLVGALVLVIFGESIGVRVADLVGLGSLFATLWSIVQWPVVALVVLFAF
                     ALIYYFAPAVEQRFRWISPGSILAFVFWLIFSLLFSLYTSIAGSYNETYGSLAGVIVL
                     MLYVYYSALIMLIGAEVNQVIEWHIPGGKNEGEKIPEADRRPDVPRMNREDGETR"
     gene            complement(8173..8358)
                     /locus_tag="VFQ10_12490"
     CDS             complement(8173..8358)
                     /locus_tag="VFQ10_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966206.1"
                     /translation="MNIRQANGIGRVHVAKPQFRETYCGRTINDEDWVTTTKEANCTG
                     CARAGAPGLERTPGASR"
     gene            complement(8386..9033)
                     /locus_tag="VFQ10_12495"
     CDS             complement(8386..9033)
                     /locus_tag="VFQ10_12495"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9966207.1"
                     /translation="MPQIPEEAFRRADETPDEEFYRTPRLVTHIDDGAIAAVTQLYRE
                     LFPAGGELLDLMSSWVSHLPPEVAYRRVIGLGMNEVELRRNEKLDSYIVQNLNTNPEL
                     PFGEAEFDGVGICVSIDYLTRPVEALREVGRVLKVEAPLIVTFSNRCFPTKAVEIWHR
                     LDDRGHMRLVESYLEEAGNFRDIRSLDRSPRRLVGDPLYAVVAESTGAYIGGLTG"
     gene            complement(9059..9667)
                     /locus_tag="VFQ10_12500"
     CDS             complement(9059..9667)
                     /locus_tag="VFQ10_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9966208.1"
                     /translation="MAEKRKYEMKKRAERHRETRRRIVEATVELHRTRGPANTTISEI
                     AQRAGVNRLTVYNHFPDTTDLLKACSRSWTERHPAPDPTPWAQISDPQERLRTALADL
                     YGFYARTEPMRANVLRDAQTMPELAALLEGTVVPYLEEARSLLAEGWEVRQQGRRRLL
                     ATLALALDFHTWRSLERGSGLSRKEAVETMLEAVRSSVSRPG"
     gene            9776..10315
                     /locus_tag="VFQ10_12505"
     CDS             9776..10315
                     /locus_tag="VFQ10_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9966209.1"
                     /translation="MAEAMNSLARRVLSPEEGEPVWLRKLGIRLMIGGEDTRGNFALV
                     ECLIGARALAAPMHTHEREEEYTYVLEGEVGVQIGEEVLVAEPGDLVFKPRGVPHAFW
                     NAADEPARALEMISPAGFERYFAELAPLFPPANQGPLDEQAVGAVREKYGLEMDMGSI
                     PVLAERHGLAIDAPPPSGK"
     gene            complement(10362..10514)
                     /locus_tag="VFQ10_12510"
     CDS             complement(10362..10514)
                     /locus_tag="VFQ10_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966210.1"
                     /translation="MDEEGAQQAQATEVLKPSGEDCEICGTEMYGLHCKLICPNCGYR
                     RDCSDP"
     gene            complement(10553..10948)
                     /locus_tag="VFQ10_12515"
     CDS             complement(10553..10948)
                     /locus_tag="VFQ10_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966211.1"
                     /translation="MYMVLLILILLGALISVVTAFRALGAYLRFRRTRAAFQGEVMDE
                     VTRLSARTDELEISLSALDARAAQLPVQISELQQSLTTLQVLTRALGASLRQVQKVLS
                     YNALKTLSAARIGKILQNASAPKDRQPSS"
     gene            11021..11094
                     /locus_tag="VFQ10_12520"
     tRNA            11021..11094
                     /locus_tag="VFQ10_12520"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11055..11057,aa:Met,seq:cat)
     gene            11334..11813
                     /locus_tag="VFQ10_12525"
     CDS             11334..11813
                     /locus_tag="VFQ10_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET9966212.1"
                     /translation="MSEESGSSQGSPLKSEMGNTTISSAVVSQIAGIAAQEVEKVQMG
                     GGASAAVGGFLQSVAGNVTGGSTSGGGNFSKGVSVEVGEEEAAVDLTMAIEYGQSIPQ
                     ITEAARRNVINRVESLSGLRVTEVNISVTDVQIPEERPMLQEQEQTEEMARRQEQRA"
     gene            11959..13245
                     /gene="tilS"
                     /locus_tag="VFQ10_12530"
     CDS             11959..13245
                     /gene="tilS"
                     /locus_tag="VFQ10_12530"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET9966213.1"
                     /translation="MDLRRPLALVSGGPDSVALLRVVVALGGEPVVLHVDHGLRGEES
                     REDAEFVRELCRSLNVRCEVRRLGLDGGSNLQERARDERYRLAGEVAAGMGLGVVATG
                     HTADDVAETVLMNLARGTGLRGLAGIPPVRGNLQRPLIGCTRREILDYLEEIDQPYRT
                     DPTNLTGKYARNRVRLEVLPVLEELYPAAASNIARGASLVREDLEVLEELATGTLERK
                     GEEVVLPLDGLMKLRPSLRRHAVRLAYTTLVPDTAPLPSKLIEAVLGLLEGGEGTRTL
                     DLPGGVVASGRSGEELAFYRGPRSMVSGSGEIRAGEPVVFGGWRISAREVSGYDPVDA
                     ARPEVAYLDAGNGPYQVRMAREGDIIRPLGLGGTKKVLRAMMDRKVPSDLRRSTPVVV
                     DGRDEVAWIVLGELDERYKVDERTEQILRLEVARIS"
     gene            13257..>13558
                     /locus_tag="VFQ10_12535"
     CDS             13257..>13558
                     /locus_tag="VFQ10_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET9966214.1"
                     /translation="MMPDVKEVLITSQEIEEKVREIGERITEDYRGENPLLIGVLRGA
                     VVVMSDLMRNIDLQCELDFMDISSYGSGTSSSGVVRILKDLEEDITDRHVLIVEDI"
BASE COUNT         2517 a         4068 c         4418 g         2555 t
ORIGIN      
        1 acggaaaccg tggtggcgca tggggtttga cccgtgagcg ccgtcctcgt cgcagaccca
       61 tttaagtctt gtgaacctat tcttcaccgc tcataagtac agtgttacgc ccgtacccat
      121 ggtaggcatg ttcgacgcgg cgatcggggc cgcagacgcg agcgccgact acgacaaggc
      181 gcggacgtgc gcagtacaga agctggaaca cttgaagagc attccgtacg aggccgtcga
      241 gggaggcccg aaggccggcg tctgcaaccc ttccggtttc gcatacacga tagccaccca
      301 gtccatcggt accgacctcg gcgacagttc cggggagacg gggctgatga tggtcaccgt
      361 tacggtcgac tgggatggcg agaactccta cgtcgtttca ggggtcgtgt ccagatggtg
      421 aacctggcaa ggagccgcct gtggaaggag gaaacgggtt tcacgctcgt ggaggtactc
      481 gtctccgcga tgatgatgtt cggcgtgctc ttcgccctgt atgcgatctt cgatgtgagc
      541 gtaagggcct tcggttacgg cggcgacagg cttgaggccg tcgggaatgc caggctcgcc
      601 cttggcagga tggaacgtga gatcagggcc gcctatccgt acgacctgac atcggacccc
      661 cccaggagat acctgtttct ggaccccacg gatcccacag ggcccgcagt gcctgcggca
      721 acccgcgtca ccttcggcaa cgagaccggg ggagaccgca ggatcgaggg ctcggaagtc
      781 ataggctatt acctcagcgg ccacgatttg aaacgttcca agggcggcag tgctcagacg
      841 ctcgttgatt cggttcccgc ggacggactg cggtttaccc cgtgcaggag tgctgacgac
      901 tgtcctccgg ctctcgccat cacggacgag gcggagatca ggctcctgcg catagagctg
      961 gccgtcgaag tcaggcgccg cggtcaagac cccgccaggc agacgctcgc caccgacgtc
     1021 tatctcagaa accgcgcaga ccaacgatga tccggcgcct cctgcgcgaa gaaaggggta
     1081 tggctctcgg gctcgcggtc atcgtcgtgg tgatcgtcgg cgtgatggga gccggccttc
     1141 tgaccttcgt cgccacggac ctggaggccg tcgtggcagt caacagggga gaacaggcct
     1201 tcgagttggc cgaagctggg atcgaggtcg ccaaagcaca cctcgcgaac gacgcgagcc
     1261 aggccgactg gtcctcgggg gaactacaca tggccagcat ggttgagggc tccgttaccg
     1321 ttaccgtcga atacgacgcc gatagcaact ccttcgaggc ggtctccata ggccagtaca
     1381 gggagacgaa ccgcaagctc gaagccacct tctccgtcga agatggcgca ccaaagctcc
     1441 tgagctggcg ggaactctac gaatgaagcc agagaaaata tcgtcaataa aggttgacaa
     1501 gtaatggatc gtcaactatg attgacgtat ggacgagaag acgaggacac gggttggtgg
     1561 gccggatgac cctcgggagg gtctcgaagc ggtcgtcgcc ttgcggcgga cgctggaggc
     1621 tttagaggcg gcgcaggtgg agaatgccat cgtcgcggga tggtcatggg cgaggatcgc
     1681 cgaggtcttg ggggtcagca agcaggctgt gcacaagaag cacgccagga ggatccggca
     1741 gaagtaccct ggagaaccac cgaggagaaa gggaaggagc ctgtgatatt cacccgcttt
     1801 tccgacagat cccgcatgtg tgtcgaggcc gccttggatg aggccaggat gcttggtcac
     1861 gattcgcttg gtgatgagga ccttttgctt ggcatcctgc gtgccgatga cgggataggc
     1921 gccgaagcac tttcctcgct cggagtgacg ctggaaggtg cgcgagagga gtcagaggag
     1981 atgctttctg gcgccctcgc ttcgataggg atctccctag aagaggtgcg cagggaggcg
     2041 ggtgatgcct tcgagttacg cattccggac aaaaggcgca tcacgatctc cccctgggcc
     2101 aagaaggcat tggtcgaagc gcgtaaagag atgcgaaggt tgggagacaa ccacctcggc
     2161 accgagcacg tcctgcttgg gatactgcgc aacgcagacg gcacggccgt aaggatgctc
     2221 tccgggatgg gcgtctcgcc tgaggtggtg gaggagcggc tcttcgagtt gcgcggccgg
     2281 gcggccagct gagaccgctc gctgtggcca aagtatctca gcacggcggc tgtgccgccg
     2341 gttgtccggc tcacgcagag ctgaaatgct gaagtgaaga caagccgcct ggcgaagcct
     2401 ggcggcttgc tgacatgctg acatgggcgg cctctggccg cccatgtcgc tagaatgctg
     2461 gcatggagga gagagaagaa cgcggtgggg ccgtcagggt cgggatgatc tggggcggca
     2521 tcggcggggt ggttggtctg ctggtctcgt tgcttggttc actggtcggg atcctggtcg
     2581 caggctttat cggcttctcc tgcgggagaa gggctgccgt tgcgggaagt cgggccggcg
     2641 cgctctccgg gctggtcggc ggggccgtcg ctgcgcccgt gtacgtgctt gggtccacgc
     2701 tcggggcgct ccttgcggcg cgccagatcg gggccgccgg gatcgccacg accctctcgg
     2761 atgtgctcgg tacgcgggtc tcggccgatg aggcctggat ctacttcctg ttcagcctca
     2821 ttctctctgc catcctcgag gccgttgtcc tgatcctggt ctcatcggcc gccggagcct
     2881 gggcgaaaag ggagtagaaa aggcctctct gcgccctgcg gctcatcgct ctctggctgc
     2941 ctccgccgcg tgctccccga aaacgaactc cccgacgtct ggccgcgtca cgccggcctt
     3001 ctccaggcgg cgatccttct ggtacttcct gatggcggcc ttcagggtcc ccagacctag
     3061 tgtcgcgcac ttggggcgag aggcgacgac ctggcgtcct agctgttcta tcatctcgtg
     3121 gtagtccaat tccagtacct gatccatggt caggtcgctc ctttgaacgt cctccaggag
     3181 catgctgctc gccgccatgc tgatcgtaca gccctcgccc tcgtaggtcg ctcgttctat
     3241 gtgctcctgg tcgtcggccg ccttgaggta gatcgtaacc acgtctccac aaccaggatt
     3301 gccgccaggc atagccacgt cggcgtcctc catggcgccc ctatgccggg gcttctcgta
     3361 gtgatccagc agtatctgta tctgtaactg cctgtccaac acgcaacctt ccacttgtca
     3421 tcccataaat tctctaaaga cgattctatc gcaagctcgt ccgccggcac gtaaacttgc
     3481 tacagtttga tacacttact tcatgaaagt aggtatagtc ggtctgccga acgtcggcaa
     3541 gtccacggta ttcaatagcc ttacgcgggc aggggccgaa gcccagaatt atccttttac
     3601 gaccatcgac ccgaacctcg gtgtcgccac aatacaggac gaacggctgg tcgccgtggc
     3661 cggggtgatc ggcagcaaac gcgtcgtccc cgcgactgtc gacttcgtgg acatcgcggg
     3721 gctcgtgggg ggcgcgagcg ccggggaggg gctgggcaat cagttcctcg gccacatcag
     3781 ggagtgcgac gccgttgcgc acgtcgtccg ctgcttcgag gacgagaacg tcgtgcacgt
     3841 ccacgccggt gtggatccca ggggcgacat cgagactatc gacaccgagc tgttgctcgc
     3901 agatctcgcg agtgtcgagc ggcggctgga gcgggtcggg cgggccgcca agggcgggga
     3961 cgccagactc gaggacgagg cggctgagtt ggaacggcta cgcgatcatc tctctgacgg
     4021 aagtccggcg cgtgcctacc cggcgacgga gacacttgcc gaggccatga gcaccctcat
     4081 aacggccaag ccgacgctct acgtggcgaa tgtggatgag gagtctgtgg cggaagggaa
     4141 ccgctacagc tcgcaggtcg agaagcacag cagcagcgag ggtgctgagg ccgtaaggat
     4201 ctgcgcgaag ctcgaagccg agatcgccga gctcccagaa gacgaggctg cagagtacat
     4261 ggcgatgctc ggggtagaga cgacaggttt cgaggagttc gtgcgggcgg cgtatcggct
     4321 gctcgggctc gtcacgttct tcaccgcggc ggagaaggag ggtcgggcct ggaccatacg
     4381 acagggctcg acggtgcggc aggccgccgg gcgcatccac accgacatgg agcgtggctt
     4441 tatcggggca gaagtcggag actggcagga tatagtcgcc gccggttcct gggccagggc
     4501 caaggaggag gcgaaggtcc gtctcgaagg ccgcgactac gtcgtaagag acggcgatac
     4561 ggtactcgtc cgcttcaacg tttgagggcg tacccggtgc agcctttcgt caagccgaag
     4621 aagtccctcg atagatcacg cctcgcgctc gtcaccacag gcggcgttca cgtgcccgac
     4681 caaccgcgct tcgatatcga cgaccctgcc ggtgattgct cctaccgtga gataccggcg
     4741 accgccaacg acctcacctg gacccacgcc tactataggc cggacgaggg cagcgacctg
     4801 gacgccgtct ttcccctgga gaccctctgt agactggtca gagatggtga ggttggcgag
     4861 ttggcaccgc gccacttctc ctttatggga gccatccacg acccggcccc gctggtagaa
     4921 agcaccgctc ccgaagtcgc cggcaaactc gtagaagacc aggtagacgt cgtgctgctc
     4981 acgccttcgt gacccctgtg ccaccggtcc gtcgggctgg tctgcaacac gatagaagaa
     5041 cgtggcatcc ccaccgtcac cctggccatg gagcatggtg tcatagcgcc acgtgtcggc
     5101 ttcgtgccct tcccgtacaa cctcccgatg ggcgaacctc atgaccgcga aactcacaga
     5161 caggtggccc tcgccgcact cgccctactc tacgatcttg agcagccggg cgaagtcagc
     5221 atttcagtca tctagctttc tgtgtgcgag tcctggccaa ccggcggcgc atccgccgtg
     5281 ctgaagtgct gacgagcgaa ggccgcaagg cctgagcgtg ctgagaggcg cgaagcgccg
     5341 tgctgacaag cggaggcgaa agccggagcg tgctcacttc ctgagatata atcgacgtac
     5401 atattgacct agtgcgaagg tgagaccgtg gccctgttgg cagtgttgtt catagcgttt
     5461 ttcgtgattg cgttcctctt cctgctgccg atactgttct cgctgcaggc cgtggggaac
     5521 cttttcgtct atccgcggca gctacgggcg atcttcggga acaagatcct gcgccgcaac
     5581 cacgccctcg agcatgcgac cgtagcggta atgatggagc gggagccagg ccgcaagctg
     5641 aatgggttct ccaccgacga cgggttcttc gtgcagggtg tacgctccat ttccgaggtc
     5701 gagagcgccg ccagagaggc cttgcgcagg ctgcagaatg gggagaagag gctcgccatc
     5761 caccgcaact gcggcacgac catcgtggcc gcgaacctgc tagcggctat cctgttcctg
     5821 gtcgccctgg tgttgggggt tctctatctt ggtgtgggtg gtgatctcta cctcatgatc
     5881 cttggcagcg tagtcctcgc tttcgccctg cgggtccctt tgagcctcct tctgcagagg
     5941 ttcgtcacca ccgatgctga cctggagaac gcggaagtcg gttgggtcga gccggcgcag
     6001 cctggcgacc tcaagagcgg tatcttcggg ctcctgctcg ccgcgtcgac cgcgcgggtg
     6061 cgcgtcttcc acaccgaccc tgacgcggtc gagatcttac gcgacgacgg cgccatagtt
     6121 cgctagaagg tatcagggca cgggataggc cctgtctgag gacacccgct ctgcggtggt
     6181 cgcccagccc tgtagccgct ctattcgtcc agcaacgccg ggtcgaggat ccaaccctcg
     6241 tcgggatcgc actccggagg tacgccgtac ccttcgtctc tcttttcgta ggcccacgcc
     6301 gcctggaccg cgcacaggag cgagtcgagg gcgtcgccgg cagggtccct tacgaactca
     6361 tccctccagc gagcatccat ctccacagcg aatccataaa cttcgctcag ggcagtcgac
     6421 tccagaccgc gcagcagcgt ctccctggcg gatctcctct ccggcgtgtc gggtgcggcg
     6481 tccctcttgt aagccgtgtg gcccaaaaag cggcgggcga cgacggcagg gtaggcctcc
     6541 acggccacgc gttcgtcccc attccgtctg cacggctcga cacggacgcc cgaggctagg
     6601 aggcgtggcg ccccctggta gaacattttg cctacgggga ccctgaagag catcatcgcg
     6661 ctgctggatc cactcctgcg gtcggcgagc cggtaacgcc acttgctgcc tgctggcctt
     6721 ttggccatgt cggcgcggat cttgtcctcg aactcctcct tcgggagcga gccgacctcg
     6781 cccacatagc cttcccaact ctccggccac ccgagggcgg ctaccaggga tcttggttga
     6841 ccgaacggga aatccattcc gcacacccat gggccacgtc tttgcaagaa gtcttcgaag
     6901 ccgccgaagt cggtaagcgt ctccgagtcc tcgacgcgca aggagcctcc ttcgagcgta
     6961 cagccgagga cgatgagggg tttccggtgg cctggggcgc tagtgaagtc gaggccgtag
     7021 atcctcacgc ggcttcgttc gaggtggacc taccgggtct cgccgtcttc cctgttcatc
     7081 cgggggacgt ccggtctgcg gtcggcttcg gggatcttct ctccctcgtt cttgccgccc
     7141 gggatgtgcc attcgatcac ctggtttact tcggccccta tgagcatgat cagcgccgag
     7201 tagtagacgt agagcatcag gacgatcacg cccgcgagcg acccgtaggt ttcgttgtag
     7261 gagccggcta tgctcgtgta cagggagaac agcagcgaga agataagcca gaacacgaaa
     7321 gcgaggatcg agccggggct gatccaacgg aacctctgtt cgacggcggg ggcgaaatag
     7381 tagatcaagg cgaaggcgaa gaggacgacc agggccacga caggccactg cacgatgctc
     7441 cagagcgtcg cgaagagtga ccctagaccg acgaggtcgg ccaccctcac gccgatagac
     7501 tcgccgaaga ttacaagcac gagcgcgccg accaggagaa cgactaccgc gagcgacatg
     7561 aagatcgaga gcccgtacac cttccagaaa ggtctatctt cctcgacctc gtacatgacg
     7621 ttcatagcct ccattaccga gcgaaacgcc ccagaaatcc cccagagcgc gagcaggatc
     7681 gagatgatcg ccccgaaagt gaaggcgctc tcagcctgac tctgggtgat gctgagaagc
     7741 tgcccctcga taaacctcgt ggccgattgc ggcgctatcc cggagagctt gtcgaccatg
     7801 acgttgacaa ggtcggtcgc gttgaagacc cccagaagcg aaagcaagaa ggtgaaaaac
     7861 gggaagatcg cgaaaaggcc cttgtaggtg aggttgccag caaatgccat gacgtggtct
     7921 tcgttcatct ccttgtaagt cagcttggag aagtcgacga gacccaggtt ctccgcgacg
     7981 gggacctcga cctcgttacc ttttctcttg gcctttttga tcagccgcat accaggtatc
     8041 ttcatgcgcc ggtcctcccg taggcgaaaa cctcagattg cttcgtcacc aagttgtacc
     8101 ctcgggaggc catccatcta acatgttgtt ccggtccaag acttgcggtc gagactctcc
     8161 cttgcccgcg gatcagcgcg acgcgccagg agttcgttct agtccaggtg ccccggccct
     8221 ggcgcaaccg gtgcagttgg cctccttggt ggtggtgacc cagtcctcgt cgttgatggt
     8281 cctgccgcag taggtctccc tgaattgcgg cttggccacg tgcaccctgc cgatcccgtt
     8341 cgcctggcga atgttcatct ctctcccttt ctctgcctgc ccagtctaac ctgtcagacc
     8401 accaatgtag gccccagtgc tctccgcgac gacggcgtag agcgggtcac cgaccaaccg
     8461 gcgggggcta cggtcgagac tgcggatatc acggaagttg ccggcttcct cgaggtagct
     8521 ttcaacgagt cgcatatggc cccggtcatc cagccggtgc cagatctcga ccgccttcgt
     8581 ggggaagcag cgattggaga aagtaacgat cagtggggcc tcgaccttga gcacccgacc
     8641 gacttcgcgc agtgcctcca cgggcctggt caggtaatct atcgagacgc agattccaac
     8701 cccgtcgaac tctgcctctc cgaaaggcag ttcggggttc gtgttcaggt tctggacgat
     8761 gtaggagtcg agtttctcgt tgcggcgcag ctccacctcg ttcataccga gcccgatcac
     8821 acgccggtat gcgacctccg gcggcagatg gcttacccaa ctgctcatga ggtccagaag
     8881 ctctcctccc gccgggaaga gctccctgta cagttgcgtg acggcggcga tcgcgccgtc
     8941 gtctatatgg gtgacaagcc gaggtgtcct gtaaaattcc tcgtccggcg tctcgtcggc
     9001 acgcctgaaa gcctcctctg gaatctgcgg caacgccttg cctccctccg gtctctcact
     9061 agcctgggcg cgacacagaa gagcgcaccg cctcgagcat tgtctccacg gcttcctttc
     9121 tgctcaagcc gcttccccgt tcgagcgagc gccaggtgtg aaagtcgagc gccaacgcga
     9181 gcgtcgcgag taggcgcctt ctcccttgtt ggcgaacctc ccagccctca gcgagcaggc
     9241 tgcgcgcctc ttcgaggtag ggcacaaccg taccctcgag cagagcggcg agctcgggca
     9301 tcgtctgggc atcgcgtagg acgttcgccc tcatcggctc ggtgcgcgcg tagaagccgt
     9361 acagatctgc cagtgccgtc ctaagcctct cttgcgggtc gctgatctgt gcccagggtg
     9421 ttggatccgg cgccgggtgc cgctcggtcc agcttctcga acatgccttc agaagatcgg
     9481 ttgtgtcggg gaagtggttg tagacggtca gcctgttcac ccccgcgcgc tgggctatct
     9541 cgcttatggt ggtgttggcg ggtccccgcg tgcggtgtag ctcgaccgtg gcctctacga
     9601 tccggcgccg cgtctcacgg tggcgctcgg cccgcttctt catctcgtac ttgcgtttct
     9661 cggccatttc aataaacact ataccaaacg atattgacac ggagatcgag taggtgtatg
     9721 gtgccgactg ttcgataaac gtagtgttta tcgaaactag cagaggaggt cgttgatggc
     9781 tgaggcgatg aactcgctgg cgcgcagagt gctctctccg gaggagggcg agccggtgtg
     9841 gctgaggaag ctcggcataa ggctcatgat tggaggagag gatacccggg gtaacttcgc
     9901 gctcgtcgag tgtctgatcg gagcgcgggc gctcgccgcg ccgatgcaca cccacgagcg
     9961 cgaggaggag tacacctacg tgctggaggg agaggttggc gtccagatag gcgaagaggt
    10021 tctcgttgcc gaaccggggg atctggtctt caaacccagg ggtgtgccgc acgccttctg
    10081 gaacgcggct gatgagccgg cacgggcgct ggagatgatc tcccctgcgg gcttcgagcg
    10141 ctacttcgcg gagctggctc cgcttttccc tcctgccaac cagggtcctc ttgacgagca
    10201 agcggtaggc gccgtgaggg agaagtacgg gctggagatg gacatgggct ctatccccgt
    10261 gctcgccgag cgccacggcc tcgcgatcga cgcgcccccg ccttccggga agtagcaccg
    10321 gcgaacaaag ggtggggccg tgggtacgct cgtgtcctga cctagggatc agagcagtcg
    10381 cggcggtagc cgcagttggg acagatgagc ttgcaatgga gaccgtacat ctccgtgccg
    10441 cagatctcgc agtcctcacc cgaaggtttc agtacctcgg tggcctgcgc ctgctgtgcg
    10501 ccctcttcgt ccatctctcg aagtatacgg cgggcctctg aaatcggcga ctctaactgg
    10561 agggctgacg gtctttcggg gccgaagcgt tctggaggat cttgccgatt cgggcggcgc
    10621 tgagcgtctt tagcgcattg taggagagaa ccttctgcac ctggcggagc gaggcgccca
    10681 gcgctctcgt cagaacctgc agagtcgtca ggctctgctg cagctcagag atctggacgg
    10741 gaagctgcgc ggcgcgggcg tcgagggcgg agaggctgat ctccagctca tcggtccgtg
    10801 cggagaggcg ggtgacctcg tccataacct cgccctggaa ggccgcacgg gtgcggcgga
    10861 acctcaggta agccccgagt gcgcggaagg ccgtgaccac cgagatcagg gctcctaaga
    10921 ggatcaggat cagtagaacc atgtacatat ggacgcctac cgtagcacgg cgctccccgc
    10981 ctttacaaac tccgggccag tgttagaatt tcgcctacga cgcggggtgg agcagtctgg
    11041 tagctcgtcg ggctcataac ccgaaggtcg caggttcaaa tcctgccccc gctacttctc
    11101 cgaggccccg caactgcggg gttattgtta ttctgggatg tcttcccgaa agtctctacc
    11161 tgacactcgt gtgggaacgc gcgggtttag gattgtgaaa ggcggggtag gcagcctgat
    11221 accgtctata gagtcgtggg gaagctatgg acccggcctc ggcgggagac gtacagggtg
    11281 cctgcgtggc gctcaggtgc cactcatctg acaaatgaac ggaaaggacg ggcatgagcg
    11341 aagaaagcgg tagcagccag ggaagcccac tcaagagcga gatgggcaac actacgatca
    11401 gcagcgcggt ggtctcccag atagcgggca tcgcggcgca ggaggtcgag aaggtgcaga
    11461 tgggcggcgg cgcctccgcg gccgtcggcg ggttcctgca gagcgtggcc ggcaatgtca
    11521 caggcggcag cacgagcggt ggtgggaact tctccaaggg cgtgagcgtc gaggtgggtg
    11581 aggaagaggc cgccgtggac ctgacgatgg ccattgagta cggccagtct ataccccaga
    11641 tcaccgaggc agcgcggcgc aatgtgatca accgcgttga gagcctctct ggcctgcgtg
    11701 tgaccgaggt gaacatatcg gtcaccgacg tccagatccc tgaagagcgg ccgatgctac
    11761 aagagcaaga gcagacggaa gagatggcta ggcgccagga gcagcgagcc tgagctaggg
    11821 cactacgccc ttaggatgga agaccggggc accacgcccc ggtcctttct tttgctagcc
    11881 cctgtacttc tctaaactat ctgcatggga tccgcgagtt tcccggataa ggtgcatcgg
    11941 actgcggagc gttacggcat ggaccttcgt cgcccgcttg cgctggtctc cggtggccct
    12001 gactcggtgg cgctgctacg ggtagtcgtc gcactcggcg gcgaacccgt ggttctgcac
    12061 gtggaccacg gtctgcgcgg ggaggaatca cgcgaagatg cggagttcgt ccgcgaacta
    12121 tgtcgcagtt tgaacgtgcg ctgcgaagtg cggcggctag ggctcgacgg tggttccaac
    12181 ctccaggaac gggcgcgtga tgagaggtac aggctcgccg gagaggtagc cgccgggatg
    12241 ggccttgggg tcgtcgcgac gggccacact gccgacgacg tggccgagac cgtgctcatg
    12301 aacctggcac ggggcacagg cttgcgcggg ctcgcaggca tcccccctgt acggggcaac
    12361 cttcagcggc ccctgatcgg ctgcacgcgc agggagatcc tggattacct ggaagagata
    12421 gaccaaccct atcgtacgga ccctacaaac ctgaccggca agtacgcccg caaccgcgtc
    12481 cgtctcgaag tcctgcccgt cctggaggag ctgtatccag cggcggccag caacatcgct
    12541 cgcggcgctt ccctcgtccg ggaggacctc gaagtcctgg aagagcttgc gaccggaacc
    12601 ttggagagga agggagagga ggtggtcctg ccgctcgacg gactcatgaa actgcggcct
    12661 tcgctccggc gccacgccgt gcgcctggca tacacgacct tggtcccgga taccgcgccg
    12721 ctcccttcga agctgatcga agcggtgctg ggactcctgg aaggcggtga gggtacgcgg
    12781 acgctggacc tgcctggtgg tgtagtcgcc tcgggccgct ccggggagga gctcgcattt
    12841 tacagagggc cgcggtcgat ggtctcgggg agcggggaga tcagggccgg ggagccggtg
    12901 gtgttcgggg ggtggaggat ctcggcgcgg gaagtctctg gctacgatcc cgtagatgcg
    12961 gcacgccctg aggtggccta cctcgatgcc ggaaatggcc cttaccaggt gcggatggcg
    13021 cgggaggggg atattatccg gccgttgggc ctcggtggga ccaagaaggt cttgcgggcg
    13081 atgatggaca ggaaggtacc gagcgatctc aggcgcagca cgcccgtcgt ggtggacgga
    13141 cgagacgagg tcgcctggat agtgctcggt gagctcgacg agcggtacaa ggtggatgag
    13201 aggacggagc agattctcag gttggaggtg gcaagaatct cgtgagaatg tccagtatga
    13261 tgcccgacgt gaaagaggtc ctcataacct cacaggagat cgaggagaag gtgcgtgaga
    13321 tcggggagcg catcaccgag gactacaggg gcgagaatcc gcttttgatc ggtgtcctgc
    13381 gcggggctgt cgtggttatg agcgacctca tgcgcaacat agacctgcag tgcgagctgg
    13441 atttcatgga cataagctcc tatggcagtg gcacctcatc gagcggcgtg gtccgcatcc
    13501 tcaaggacct cgaagaggac atcacagacc gtcacgtcct gatcgtcgag gacatcat
//
LOCUS       DASTKM010000316         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1368591, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000316 DASTKM010000000
VERSION     DASTKM010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368591"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="VFQ10_12540"
     CDS             complement(<1..1533)
                     /locus_tag="VFQ10_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9966215.1"
                     /translation="MASNSSASSDTGDLFQRKATGLLKGWSAWDGFIYATFSINLITL
                     GFLALSYAPFIPNGSLILAIILAGLYLIFQTVTYAALIAVMPRAGGDYVWTSRVLGGS
                     IGFVLAVTGWWFILWHWVPIYADLLNKEVFVPMAAILNLDGVASYFGGATGLFVSCMI
                     VALLASLFIALGMRAYARIQKFCFYGALVGLAVMIVVLLVSSRSDFVSAYNAGAQELF
                     GAGSDAYQKTIEAGSVEISSAFYPTLLLIPFLAFFNLYSNWGATLYGEVRGASDFRKN
                     IYAMAGALVFTSTAAVILLLLFAKTFGWDFYYAANAAFWAGEGPVLAWPYPGLFAALL
                     LGSPVLQVILVALLSLWFFGWVGTVFLSSTRVVFATAFDRVLPEWVAKTSRNGVPYAA
                     LLLMLIPSIPISYWYAFGEDFATWTLAATLVIAVTFLGSAVSAAVLPWRKPDIYNASP
                     IARYNVLGIPLITIAATAFGVFLVFCLYQWFFRDIYGLNNTASFIYISAMYVLALVMY
                     VGF"
     gene            complement(1553..>2042)
                     /locus_tag="VFQ10_12545"
     CDS             complement(1553..>2042)
                     /locus_tag="VFQ10_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966216.1"
                     /translation="KRVQLAEGKVVEFGDFQMVSTGWSNRTPWDTYREEDEEDLAIRL
                     KKMTGQVTASPEKTIYNFHCPPYGSGLDDAPELTEDLRPKHGGRSIVPVGSTAVRDAI
                     KEGQPALALHGHIHEAKGNTRIGRTLCINPGSSYEQGQLLGAVVNLDGKGKVKRFVLT
                     SG"
BASE COUNT          448 a          623 c          625 g          346 t
ORIGIN      
        1 gaagccgacg tacatcacga gcgcgagcac gtacattgca ctgatgtaaa tgaacgaagc
       61 agtattgttg agcccgtaga tgtcgcggaa gaaccactgg tagaggcaga agaccaggaa
      121 cacgccaaag gcggtcgcag caatcgtgat caggggtatg cccagcacgt tatagcgggc
      181 tatgggtgag gcgttgtaaa tatccggctt gcgccacggc agcaccgccg ccgagaccgc
      241 cgagccaagg aacgtcaccg cgatgacgag cgtggcagcc agcgtccagg tggcgaagtc
      301 ttctccgaaa gcgtaccagt agctgatcgg gatggagggt atgagcatga ggagcagggc
      361 ggcgtagggt acgccgttgc gcgaggtctt tgcgacccac tcggggagca cccggtcgaa
      421 ggcggtggcg aagaccacgc gcgtcgagga gaggaacacc gtgccgaccc acccgaagaa
      481 ccacagcgac agcaaggcta ccaggatgac ctgcaaaacg gggctcccca agaggagtgc
      541 agcgaacaat ccaggatagg gccacgctaa gaccggacct tccccggccc agaaggcagc
      601 gttcgccgcg tagtagaagt cccacccgaa ggtcttggcg aacagcagga gcaggatcac
      661 agccgccgtc gaggtgaaca cgagagcccc agccatcgcg tagatgttct tgcggaagtc
      721 tgaggctccc ctcacttcgc cgtacaaagt ggcaccccag ttcgagtaga ggttgaagaa
      781 ggcgagaaac gggatcagaa gcagcgtcgg atagaaggcg gagctgatct cgacgctgcc
      841 ggcctcgatg gtcttctggt aggcgtcgct ccccgcgccg aagagctctt gggctccggc
      901 gttgtaggcc gacacgaagt ccgatcgcga gctgacgagc aggacgacga tcatgaccgc
      961 cagcccgacg agagcgccgt agaagcagaa cttctgtatg cgagcgtagg cccgcatccc
     1021 gagagcgatg aacagcgagg ccaaaagcgc gacgatcatg cacgagacga aaagccccgt
     1081 ggccccgccg aagtagctgg caaccccgtc caggttcagg atcgcggcca tcggcacgaa
     1141 gacctccttg ttcaggagat ccgcgtagat gggtacccag tgccacagga tgaaccacca
     1201 tcctgttacc gccagcacga agccgatgct accgcccaaa acgcggctcg tccaaacgta
     1261 atcaccgccc gccctgggca ttaccgcgat gagagcggca tacgtgacgg tctggaagat
     1321 cagatagagt cccgccagaa taatcgccag gatgagcgat ccatttggta taaacggagc
     1381 gtaagacaag gctaagaacc cgagggtgat caggttgatc gagaaggtcg cgtagatgaa
     1441 gccgtcccac gctgaccatc ctttaagaag acctgtcgcc ttcctttgga atagatcccc
     1501 agtgtcggag cttgctgaac tgttgctcgc cattcttcct ctcctccccc tcttatccgc
     1561 tggtcagtac gaaccgcttg accttccctt tgccgtccaa attaaccacc gcgccaagta
     1621 gctgcccttg ctcgtaggag cttccggggt tgatgcacag cgtgcgcccg atgcgggtgt
     1681 tgcccttcgc ctcgtggatg tgcccgtgca acgccagggc cggctgtccc tcctttatcg
     1741 cgtcccttac cgccgtcgat cccaccggca cgatcgaacg cccgccgtgc ttggggcgca
     1801 ggtcctcggt caactccggg gcgtcgtcga gacccgagcc gtagggcgga cagtggaagt
     1861 tgtagatggt cttctctggc gaggcggtca cctggcccgt catcttcttc aaacgtattg
     1921 ccaggtcctc ctcgtcctcc tcgcggtaag tgtcccaggg cgtgcggttc gaccaccctg
     1981 tcgagaccat ctggaagtcg ccgaactcga cgaccttgcc ctcggcgagc tgcacgcgct
     2041 tg
//
LOCUS       DASTKM010000317         6895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_663791, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000317 DASTKM010000000
VERSION     DASTKM010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663791"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFQ10_12550"
     CDS             <1..376
                     /locus_tag="VFQ10_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HET9966217.1"
                     /translation="SLGVGLLYSQTAPEIWQTMVFTTLTLSQMSHIMAIRSGNESLFK
                     VGLLSNKTLLGAVALTFVLQLLAIYAPFFQGFLDTKALPVADLAIAIALSTIIFWAVE
                     IEKWLARRKYSRQGFDAEGLVR"
     gene            667..2019
                     /locus_tag="VFQ10_12555"
     CDS             667..2019
                     /locus_tag="VFQ10_12555"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET9966218.1"
                     /translation="MEDPTLISRWQFAFTIVYHYLFPQLTMGLALLLVVLKTLYLRRG
                     DEAYNVAARFWGKIFAITFVMGVVTGIPMEFQFGTNWAAFSAFAGDIIAQTLAMEGAF
                     AFFLESAFLGIFLFGERRFGQRVHWFSALMVFVGTWASGYFILATNSWMQNPVGYEEV
                     AGGGVRISDYWAVLLNPYVFVQFAHNQGGAVVTGAFAMAGLGAYYLLARQHEHFGRMF
                     VRVGLIAAVIGSVWMLFPTGHLASEQVAEKKPVALAAMEGQFETEQPAGLVIVGQPDM
                     ERERIDNPIVIPRALSFLVYQNWGAEVKGLEAYPQVNWPDNIPLLYYSYHVMVGLGTI
                     FIAITVVAVFLLWRRKLFESRWMLWILMLATPFPFIANTAGWLTAELGRQPWLVYGLL
                     RTQEGVSPLVSSGNVLFTLIGFAGMYLIMGLLYFVLMVREVAHGPEAEEEPSGESLTT
                     "
     gene            2032..3072
                     /gene="cydB"
                     /locus_tag="VFQ10_12560"
     CDS             2032..3072
                     /gene="cydB"
                     /locus_tag="VFQ10_12560"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET9966219.1"
                     /translation="METLWFVLVAFMLTMYVLLDGFDLGAGAIHTFIAKSDEERRLVL
                     KSIGPVWDGNEVWIIAAGGTLFFSFPLLYASGFSGFYLPLIIVLWLLMIRGLSIELRG
                     HIESPVWATFWDGMFFLGSSLLSIFYGVALGNVVRGVPLGEDGYFFQPLWTNFSPTNP
                     NPGILDWYTILIGLLALVALVVHGASWVAVKTEGELNARARRAARIGWVALVALTAIT
                     TAATFSLRPGMLSSFSERPWGFVFPSLAVAGLVLVGYFGFRRRDRPAFLSSGAYIAGM
                     MASTAFAIYPKVLPAVEPENSLTVHNASGSTYGLTVGLIWWTIGMVLAAIYFILIYRL
                     FRGKVSLEDDGY"
     gene            3062..4822
                     /gene="cydD"
                     /locus_tag="VFQ10_12565"
     CDS             3062..4822
                     /gene="cydD"
                     /locus_tag="VFQ10_12565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HET9966220.1"
                     /translation="MGISPSGRSSLDGELFRQVDSARLFVGASVALGVLSAAATVAQM
                     IFLSQIIDRVFLKGASPADVRGPLLLLLGAILARAALLWTREVLAGRGALRVKSALRE
                     RMYAHLLRLGPAFLAGERAGELATTATEGVERIEPYFARYLPQVALSGAVPLLVAAYV
                     FPRDLSSGVLLLITAPVIPVMMVLVGSYAEEHINRQWTALSRMGAHFLDALQGLTTLK
                     AFGREAAEGERVAAVSEEFRERTMRVLGFAFLSGLVLEFTTAVSIALIAVVLGVRLVS
                     GNVPFEDALVVLLLAPEFYRPLRELGTHRHAAMEGKAAAKRILGVLDTPVPTNDSHVA
                     PTARISGAPRIELSGVGFAYPRSEIPALSGVNLVLEPRTLTAIVGRSGAGKSTLINLL
                     MRFADPSEGIILADGAPISALSTARWRENVALVPQSPHLFFGSLLENIRLARPEASRE
                     EVEEAARLAGAHEFVERLPGGYETQIGERGKRLSGGEAQRVAIARALLKDAPVLIMDE
                     PTSNLDPESERLIRLALERLVEDRTALIVAHRLNTVHTADRIVVLEEGWVAEAGTHEE
                     LLRRNSHYARLVGAQEGART"
     gene            4819..6564
                     /gene="cydC"
                     /locus_tag="VFQ10_12570"
     CDS             4819..6564
                     /gene="cydC"
                     /locus_tag="VFQ10_12570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HET9966221.1"
                     /translation="MRTIFRLLGLLGPYRFRVALAVLLGVATVAGNVGLLSTAAYVIS
                     AAAVVSYISLLTIPIYLVRFFSVSRSFSRYFERLVSHDLTFRLLGNLRSWFYVRLTPL
                     APALLEGYRSGDLLSRLVEDVEELENLYLRAVSPVLVAAVVWGLAFAVLYPFDPALAI
                     TVLVFLAAAGVGAPLLVWALSRGLGRRQLELRSELYSRIVEGTQGVQDLLAFGREEEQ
                     RRQIEVLNRKLGRIERRQALISGLQDSLGDLLTNLAMLVALVLAIPLVAGGEVRGVYL
                     AFLALVALGVFEAATPLGGAFQTIGRTTAAGERLFEVSDSEPTIRNPKSPLPVPEDFT
                     LRFDNVSFRYGEGDPFSLEDVSFALEPGRKVAVVGPSGSGKSTLANLILRFRDPQGGE
                     IRLGGRSLSEYAQEDVRRLVSVVPQSTHAFNDTLRNNLLLADPEANDEALELALERAR
                     ISSFIERLPDGLDTYVGEHGSRLSGGERRRLAVARALLKDAPLLVLDEPTANLDTVTE
                     LEVLASVWEAARDRAALLLTHRLVGMEEMHEILVMDAGRIVERGAHEQLLAAGGLYCR
                     MLETQRELLVVPHAS"
     gene            complement(6605..>6895)
                     /locus_tag="VFQ10_12575"
     CDS             complement(6605..>6895)
                     /locus_tag="VFQ10_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966222.1"
                     /translation="EIRFVHRDLLQGPLAVPPGCRLGRLEPQAAGRDDDAGVHADTLV
                     EYVSLADHAAGARGSINRLGVYPSRSVLLRARIFVGPNGRSRGARYAVLVGD"
BASE COUNT         1059 a         2261 c         2246 g         1329 t
ORIGIN      
        1 gtcgctgggc gtcggtctct tgtactcgca gaccgcgccc gagatctggc agaccatggt
       61 cttcaccacc cttactctct cccagatgag ccacatcatg gccatccgct ccggcaacga
      121 gtcgctcttc aaggtcggcc tgctctcgaa caagacgctg ctcggggcgg tcgccctcac
      181 cttcgtgctg cagctcctgg cgatctacgc ccccttcttc cagggcttcc tggacaccaa
      241 agcgttgccc gtagctgatc tcgccatcgc catcgcactg agcaccatca tcttctgggc
      301 cgtggagatc gagaagtggc tggcccggag gaagtattct cgccaggggt ttgatgcgga
      361 gggtttggtg cgatgagtag tccggtgaat tgcttggaca aaccgggcga gaaagcgaaa
      421 tagatagagg tgaaaatgac acatgctgaa cgcctggcgg agttcgtggt gcgctccccg
      481 tctccgccag ggacacggag caacaacgtc cgggcgctgg cggccttcac ggaggaaatt
      541 tgatcgagaa cccgtgggga tctacttaac agcgccgaac ccagagtagg gttaaggttt
      601 gtaggacagc gcgccgcacc gggcgttcga ccggcgcggc tagaaagcgg ccacggagga
      661 aacgcaatgg aagatccgac gctcatctcc cgctggcagt tcgccttcac catcgtctac
      721 cattacctct tcccgcagct gaccatgggg ctagcgcttc tgctggtcgt cctcaagacg
      781 ctgtacctgc gtaggggaga cgaggcgtac aacgtcgccg cgcggttctg gggcaagatc
      841 ttcgccataa ccttcgtcat gggcgtcgtc accggcatcc ccatggagtt ccagttcggc
      901 accaactggg cagccttctc cgccttcgcc ggcgacatca tcgcccagac tctggcgatg
      961 gagggcgcct tcgccttctt cctcgagtcc gccttcctgg ggatcttcct cttcggggag
     1021 cgaagattcg gtcagagagt ccactggttt tccgccctga tggtcttcgt gggcacctgg
     1081 gcgtcaggct acttcatcct ggcgaccaat tcctggatgc agaacccggt cggttacgag
     1141 gaagttgccg gcggcggggt gcggatctcc gactactggg cggtgctctt gaacccctac
     1201 gtgttcgtcc agtttgccca caatcagggc ggggccgtgg tgaccggtgc cttcgcgatg
     1261 gcgggcctcg gagcctatta tctgttggcc cgccaacacg agcacttcgg gcgcatgttc
     1321 gtccgggtag ggctcatagc cgccgtgatc ggtagcgtct ggatgctctt cccgaccggg
     1381 cacctcgcca gcgagcaggt cgcggagaag aagccggtgg ccctggccgc gatggaggga
     1441 cagttcgaga ccgagcagcc agccgggctc gtcatcgtcg gccagccgga catggagaga
     1501 gaacgcatag acaatcccat cgtcatcccg cgcgccctga gcttcctcgt ctaccagaac
     1561 tggggcgccg aggtgaaggg cctcgaagcg tacccgcagg taaactggcc ggacaacatc
     1621 ccgcttctct actactccta tcacgtcatg gtggggttgg ggactatctt catcgcgatc
     1681 accgtggtcg cggtgttcct actgtggcgc cgcaagctct tcgagtcgag gtggatgctc
     1741 tggatcctga tgctcgctac gcccttcccg ttcatcgcca acaccgctgg ctggctcacc
     1801 gccgagctgg gccggcagcc ctggctcgtc tacggccttc ttcgtaccca ggaaggcgtc
     1861 tcaccattgg tctcctcggg caacgtcctt ttcaccctca tagggtttgc ggggatgtac
     1921 ctgatcatgg gcctcctcta cttcgtgctc atggtgcgcg aggtggccca cggaccggag
     1981 gccgaggaag agccgtcggg cgagagtctg accacgtgag aggaggatga agtggagacc
     2041 ctgtggttcg tactggtcgc gttcatgcta accatgtacg tcctgctgga cggtttcgac
     2101 ctcggggccg gcgccataca tacgtttata gcgaagagcg atgaggagcg tcgcctcgtc
     2161 ctgaagtcca taggcccggt gtgggacgga aacgaggtct ggatcatcgc cgcaggaggg
     2221 acgctgtttt tctcgttccc gctgctctac gcctcaggct tctccggctt ctatctgccg
     2281 ctcatcatcg tgctgtggct cctgatgatc cggggcctct ccatcgagct tcgcggccac
     2341 atagagagcc ccgtgtgggc caccttctgg gacgggatgt tcttcctcgg cagctcgctt
     2401 ctgtcgatct tttacggcgt ggctttgggc aacgtggtgc gcggcgtccc gctcggggag
     2461 gacggctact tcttccagcc gttgtggacc aacttcagcc ccacgaaccc gaaccccggc
     2521 atcctcgact ggtacacgat cctgatcggc ctgctggcgc tcgttgccct cgtcgtccac
     2581 ggcgcaagct gggttgccgt gaagaccgag ggcgaactca acgcccgcgc ccggcgcgcc
     2641 gctcggatcg gctgggtggc gctggtagcc ttgacggcga tcaccaccgc cgcaacgttc
     2701 tccttgaggc ccgggatgct ctccagcttc tccgagcgtc cgtggggctt cgtcttcccc
     2761 tccctcgccg tcgcgggcct cgtcctcgtg gggtacttcg gcttcaggcg gcgcgatagg
     2821 cccgccttcc tctcctccgg cgcctacatc gcgggcatga tggcttccac cgccttcgcc
     2881 atctacccga aggtcctgcc cgccgtggag ccggagaaca gcttgaccgt ccacaacgct
     2941 tccggctcga cctacggcct cacggttggc ctcatctggt ggaccatcgg catggtgctc
     3001 gccgccatct acttcatcct catctaccgt ctcttccgcg gcaaggtgag cctggaggac
     3061 gatgggtatt agcccttcgg gccgttcgtc tttggacggg gagctcttcc ggcaagtcga
     3121 ttcggcccgg ctcttcgtgg gcgcgtcggt ggcgctcggg gtcctctcgg cggccgccac
     3181 ggtggcgcag atgatcttcc tgagccagat catagaccgc gtgttcttga aaggggcgag
     3241 ccctgctgac gtgcgaggtc cgctgctcct gctgctcggc gccatcctcg cgcgggctgc
     3301 gctcctgtgg acgcgggagg tgctcgccgg gcgcggggcg ttgcgggtga agtccgcgct
     3361 cagggagcgc atgtacgctc acctcctgcg gctgggaccg gccttcttgg cgggcgagag
     3421 ggccggggaa ctggcgacca cggcgacgga aggggtggag cgcatcgagc cgtatttcgc
     3481 ccggtatctg ccgcaggttg ccctgagtgg ggcggtgccg ctgctggtgg ccgcctacgt
     3541 cttcccgcgc gacctctcca gcggcgtttt gctcctgatc acggcgccgg tgatcccggt
     3601 gatgatggtc ctggtcggaa gctacgccga ggagcacata aatcgccagt ggaccgccct
     3661 ctcccgcatg ggtgcccact tcctcgatgc cctgcagggg ctgaccacgc tgaaggcttt
     3721 tgggagagag gctgcggagg gggagcgggt cgcggcggtg agcgaggagt tccgggagcg
     3781 cacgatgcgg gtactggggt tcgcgttcct ctccgggctc gtgctggagt tcacaacggc
     3841 ggtctccatc gcgctcatcg ccgtcgtgct cggagtgcgc ctcgtttcag gtaacgtgcc
     3901 gttcgaggac gcgctggtgg tgctactcct ggcgccggag ttctaccggc ccttgaggga
     3961 gctcggcacg caccggcacg ccgccatgga gggcaaggcc gccgcgaaaa ggatactcgg
     4021 tgtcctggac acccccgttc cgaccaacga cagtcacgta gctccgaccg cccggatctc
     4081 cggcgctccc cggatcgagc tctccggcgt cggtttcgcc tacccgagga gcgagattcc
     4141 ggcgctctcg ggagtcaatc tggtcctgga accccggacg ctcacggcca tcgtcgggag
     4201 gagcggggcc ggaaagagca cgctaatcaa cctgttgatg cgcttcgccg atccttcgga
     4261 ggggattata ctcgccgacg gagctccgat ctctgccctc tctaccgcac ggtggcgcga
     4321 gaacgtggcg ctcgtgccgc aaagtccgca cctgttcttc ggcagcctcc tggagaacat
     4381 ccggctcgcg cggcccgagg cgagccgcga ggaggtcgag gaggcggcga ggctcgccgg
     4441 cgcccacgag ttcgtcgagc ggctgcccgg cggatacgag acgcagatcg gggagcgcgg
     4501 caagcgccta tcgggcgggg aggcgcaaag ggtggcgata gcccgcgccc tgttgaagga
     4561 cgcgccggtc ctgatcatgg acgagcccac ctccaacctc gacccggaga gcgagcgcct
     4621 cataaggctc gccctggagc ggctcgtcga agaccggacg gcgctgatcg tggcccaccg
     4681 cctgaacacc gtgcataccg ccgaccgcat agtcgtgcta gaagagggct gggtagcgga
     4741 ggccgggacc cacgaggagc tcctccggcg caattcccat tacgcccggc tcgttggcgc
     4801 ccaggaaggg gcgcgaacgt gaggaccatc ttccgcctgc tcggcttgct gggaccctac
     4861 cgcttccgcg tggccctcgc ggtgctgctg ggggtcgcga ccgtggctgg caacgtgggc
     4921 ctgctctcca cggcggccta cgtgatctcc gccgccgcgg tggtctctta tatctcgctg
     4981 ctcacgatcc ccatctacct ggtccgcttc ttcagcgtct cccgctcgtt ctcgcgctac
     5041 ttcgagcggc tcgtctccca cgacttgacc ttccggctgc tcgggaatct gcggtcgtgg
     5101 ttctacgttc gcctcacgcc gctggctccc gccctccttg agggctaccg tagcggggat
     5161 ctcctctctc gcctcgtcga ggatgtcgag gagctggaga acctctacct gcgagccgta
     5221 tctccggtcc tggtcgctgc ggtcgtctgg gggctggctt tcgcggtcct gtatccgttc
     5281 gatccggcgc tcgccatcac ggtccttgtg tttctcgccg ccgcaggcgt gggagcgccg
     5341 ctcctggtat gggcgctctc gcgggggctc ggccggcggc agctggagct gaggtcggag
     5401 ctctactccc ggatcgttga agggacacag ggcgtgcagg atctgctcgc cttcgggcgc
     5461 gaggaggagc aacgccgcca gatcgaggtc ctcaaccgca agctcgggag gatcgagcgt
     5521 aggcaggccc tcatctcggg gctccaggac tctttgggcg acctgctgac caacctggcg
     5581 atgctcgtgg cgcttgtcct tgcgataccg ctggtggccg ggggcgaggt ccgcggcgtc
     5641 tacctcgcct ttctcgcgct cgtcgcgctg ggcgtcttcg aggccgccac accgctcggc
     5701 ggcgccttcc agaccatcgg gcgcaccacc gccgccggag aacgtctctt cgaggtctcc
     5761 gactcggaac cgacgatacg caatccaaag agtcccctcc ctgtgccaga ggacttcacc
     5821 ctacggttcg ataacgtgag cttccgctac ggggagggcg accccttctc gctcgaagat
     5881 gtgtccttcg ccctggagcc tggacgtaag gtggcggtag tcgggccttc gggatccggc
     5941 aagagcacgc tggcaaacct gatcctgcgc ttccgggatc cgcagggagg cgagatccgg
     6001 ctcggcggtc ggagcctgag cgagtacgcg caggaggacg tgcgccgcct cgtctccgtc
     6061 gtgccgcaga gcacgcatgc cttcaacgac accctgagga acaacctgct gctcgcggac
     6121 ccggaggcga acgacgaagc gctggagctg gcgttagagc gagcgcggat ctccagtttc
     6181 atcgaacggt tgccggatgg tctggacact tacgtcggag aacatgggtc caggctctcc
     6241 ggcggcgagc ggaggcggct cgctgtggcg cgcgccctgc tcaaggacgc cccgctgctg
     6301 gtgctcgacg agcccacggc gaatctggac accgtgaccg agctggaggt gctggcctcc
     6361 gtgtgggagg cggcacggga tcgcgcggca ctcctgctca cgcatcgtct ggtgggtatg
     6421 gaggagatgc acgagatcct ggtcatggac gccgggcgca tcgtcgaacg gggtgcccac
     6481 gagcaactgc ttgcggccgg tggcctatac tgccgcatgc tggaaacgca gcgggagttg
     6541 ctcgtagtgc cacatgcttc gtgaaccctc agaaggcgac acttcctggc tctgctccta
     6601 ctcatcagtc ccccaccaga acagcgtatc gagctcctcg ggatctcccg ttcggcccca
     6661 caaagatgcg ggcccgtagc agcaccgatc tggaaggata caccccaagg cggtttatcg
     6721 agccgcgggc gcccgctgcg tggtcggcga gggaaacgta ctcgaccagg gtatccgcgt
     6781 gaaccccggc gtcgtcgtct cgccccgcag cttgaggttc taacctcccc aaccgacaac
     6841 caggagggac ggccaaaggc ccttggagga gatcccggtg tacgaacctt atctc
//
LOCUS       DASTKM010000318         5722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_133355, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000318 DASTKM010000000
VERSION     DASTKM010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133355"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VFQ10_12580"
     CDS             complement(<1..882)
                     /locus_tag="VFQ10_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9966223.1"
                     /translation="MSAKMRDPAHLCLHELFEEQARRTPDALALEDASISLTYSELDR
                     LAGRLAAYLRSLGIGPDEPVGVYMERRAEYVVACLAAMKAGGAYLILELAYPPPMLAD
                     VVADASPRVVLTQERYAERLPEGTETFFLDEGWEEGLEDVPAADGGPEIDQDNLAFVS
                     YSSGTTGKPKGIANPHRAPVRSYLWRFGISDYAPGDRVGCNVFFIWEMMRPLLRGATT
                     VVIPDDVIYDPKALIRFLEEYGITETLVTPSLLEAVLNSSGPDVGERLAKLRTLWLNG
                     EVVTRTLARRAMDLLPDA"
     gene            complement(1423..1896)
                     /locus_tag="VFQ10_12585"
     CDS             complement(1423..1896)
                     /locus_tag="VFQ10_12585"
                     /inference="COORDINATES: protein motif:HMM:NF017941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET9966224.1"
                     /translation="MPQRQSPARRVAIAGVVGALYVVLSLAVAPIAFGPIQFRLGEAL
                     KPLVIKYPVVIPAFGIGTVIINLFSPFAGALELVFMPLVDVTGGVLCYLVARKIGGAL
                     GTYLASLLYALYTAAGVGVVLTVAAGLPYLAAFASVAASEIVLLLLGNALLVRRI"
     gene            complement(1905..3509)
                     /gene="ggt"
                     /locus_tag="VFQ10_12590"
     CDS             complement(1905..3509)
                     /gene="ggt"
                     /locus_tag="VFQ10_12590"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9966225.1"
                     /translation="MSQRLEGATRRPDLGGRSTVFGTKGAVACEHPGAAIIGLRVLDG
                     GGTAADACVAMAAAMAVLSPMQTGMGGDAFLLFYEAETGRVTGVNGSGRASRDLTVAK
                     LSHLGELPERGGLTVTVPGAVRLWEDAVYRFGNQPLARLLEPAWELAENGYPVGEVIA
                     RYWDEHEEVLRTNDAATRTFLPEGRAPVAGEIFSQVDLAATISAVAERGADAFYEGEI
                     AASIARSTQEAGGYLSEEDLAAHESTFVEPISTDYRGVRVYEMPPPGQGVAALEMLNI
                     LEGFDLASMEPSGAERIHLEVEAKKLAFEDLHGRIGDPEFSEIQTEELLSKEYAAKLR
                     GSISSDAAAVISPPDPGGDTTYICAVDAEGNGCSFINSLYKGFGSGIVAEGTGVCLHN
                     RGNSFRLEPGHPNAIAPGKRPMHTLIPGLVSSDGALWAVFGVMGAAMQPQGHAQVLVD
                     LLDYDMDPQAAVEHPRHRHENGVLLIEGRVPEEEIASLRGRGHRIEVREDFMIPAGGA
                     QLIRILDNGVRACGSDPRKDGCALAQ"
     gene            3609..3767
                     /locus_tag="VFQ10_12595"
     CDS             3609..3767
                     /locus_tag="VFQ10_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966226.1"
                     /translation="MIGGKGIYVGGTLISILISLALYFSGRRESAIFVGLWAPTIMNL
                     GQSLQEED"
     gene            complement(3837..5447)
                     /locus_tag="VFQ10_12600"
     CDS             complement(3837..5447)
                     /locus_tag="VFQ10_12600"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET9966227.1"
                     /translation="MGRRRACNKIRASRRWPSGLLRPKPPRSELRSVAGGGVSVVLAQ
                     DRDRIAREPAYHYLLRREFEEHGTKIRSLNDRGDDSPEGELTDGILDQLAKFERAKTA
                     ERTRRGRLRKAREGKVVATRSPRYGFKLNAARDAYEVDEEKMAVVRRIFREVADGTTL
                     RAIALKLEREGVPTPRGAKFWDRAFSRHCVFDDVYRPHSFDEVEAVVSPQVAARLDPS
                     KEYGLWWFNRRRMTSTLVSEASGDGRRYGKRYSSYTKPKEEWIAVPVPDAGVPREIVD
                     VAREQIKDNRRPSSAGHRFWELSGGIGRCAACNRVLISRKRSKKKGGRRYQYFYYICS
                     GYNKHGFKACEKARFASATKLEGQVWEFVRGILLDPAELRSDLDQAIELEREGRYGDP
                     EAEAKHWLEKLSEIDEERRGFLRLAAKGRIRDEELDEELAALEETRQTAEWELKDLRR
                     HKERVEQMEQDRDAVLEHYASLAPEVLDSLISEKRYHLYKMLKLKVWLAKSGDLEIEM
                     AGVPVDGLEGSSSITQGTSRSAPMGGRS"
     gene            complement(5502..>5722)
                     /locus_tag="VFQ10_12605"
     CDS             complement(5502..>5722)
                     /locus_tag="VFQ10_12605"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9966228.1"
                     /translation="ERDMVVAHWRAKATHQGEYMGIPPTGNRVNFRGISIYRIEGGQI
                     AESWSVSDLLGMMQQLGAVREPGQPQGA"
BASE COUNT         1041 a         1830 c         1709 g         1142 t
ORIGIN      
        1 cgcatctggc aacaggtcca ttgcgcgccg cgcgagcgtg cgagttacga cctcgccgtt
       61 cagccaaagc gtcctgagct tcgcgagcct ctcgcccacg tcgggcccgc tcgagttgag
      121 gacggcttcg aggagtgatg gggtaaccag agtctccgtg atcccgtact cttcgagaaa
      181 gcggatcagg gccttcgggt cgtagataac gtcgtcgggg atgacgaccg tggtagcccc
      241 gcggagcagc ggccgcatca tctcccagat aaagaacaca ttgcacccga ccctgtcgcc
      301 cggggcatag tcgctgatac cgaagcgcca caggtaagag cggaccggcg ccctgtgcgg
      361 gttggctata cccttcggct tccccgtcgt ccctgaggag tacgagacga aggccaggtt
      421 gtcctggtcg atctcgggcc cgccatctgc tgccggaacg tcctccagtc cctcctccca
      481 gccctcgtcc aggaagaacg tctctgtccc ttctggcaga cgctccgcgt accgctcctg
      541 cgtgagcacg acgcgcgggc tcgcgtcagc tactacatcg gcgagcatgg gcggcgggta
      601 ggctagctcg aggatcaggt aagctccgcc agccttcatg gcggcgaggc aagcgacaac
      661 gtactcagcc cgccgctcca tgtaaacacc gacgggttcg tccgggccga ttcccaggct
      721 cctgagatac gccgcgagcc tgccggcgag cctgtccagc tcgctgtagg tcagggagat
      781 gctggcgtcc tcgagcgcga gtgcgtcagg cgtgcggcgc gcctgctcct cgaagagctc
      841 gtgcaggcag aggtgagcgg ggtccctcat cttagcgctc acaccggtca cactcggata
      901 cgaaaagtac cgagatgcct gcacagatac cagatgtgtc cacgtctacc gtcgagccca
      961 acgaccttcg cataaagatc aaaccctcct tgcgtttcct acaccgacaa cgcgttgcca
     1021 tagaccccac agggtgcgac agaacgccgc agtagagatg atgcggacca tgctccggtc
     1081 gaacacccgg agcgaagcgg tccggaaacg ttgcatcaca tactaacccg cccgcgtgtt
     1141 agttccagca gctattctga agatgggttt aaagttgtat taagttttgt taatgtaagc
     1201 aattcagccg gtcagcactt cagtttgtca gcaatgttgg ggagttgctc gtgtttgtcg
     1261 tgctctctgt gcggtggaaa aggcaccgca tcgtttgtca tggagcctgg ctgcgtatgg
     1321 gagcatcgct ggcaggtcct tgctgagggg ctgacgaggc gctggcgaag tctggcagcg
     1381 tgctgacttg cgcgcgaggg gaaggggtga tccttcccgc tatcagatcc tgcgcaccag
     1441 aagggcgttt cccaggagga gtagaactat ttccgaggcg gcgaccgagg cgaatgcggc
     1501 caggtaaggc aacccggcgg cgacggttag caccaccccg acgccggcgg cggtgtagag
     1561 ggcgtagagt agtgaggcga ggtaagttcc gagcgccccg ccgatcttcc ttgccacgag
     1621 atagcagagc acaccgccgg tgacgtctac gagcggcatg aagaccagct ccagcgcccc
     1681 tgcaaacggg ctgaagaggt tgatgatcac ggtccctatt ccgaatgcgg ggatgaccac
     1741 ggggtatttg atcacgagcg gtttgagggc ctctccgagc ctgaactgta tgggcccgaa
     1801 ggcgattggc gcgacggcga ggctcaagac cacgtatagc gccccgacga cacctgcgat
     1861 cgcgaccctg cgggctggac tctgcctctg gggcatcact ccccctactg tgcgagcgcg
     1921 cagccgtctt tacgcgggtc gctgccgcac gctctgacgc cgttgtccag gatgcggatt
     1981 aactgggcgc ctcctgcggg gatcataaag tcttccctaa cctcgatgcg gtgacctcga
     2041 ccgcgcaggc tggctatctc ctcctcgggg acgcgaccct cgattaggag gacgccgttc
     2101 tcgtggcggt gacgaggatg ctcgaccgcc gcctgagggt ccatatcgta gtccaggagg
     2161 tctaccagta cctgtgcgtg gccctggggc tgcatcgctg cgcccatcac gccgaagacg
     2221 gcccacaggg ctccgtcgct ggaaacgagg ccaggaatga gggtgtgcat cgggcgcttc
     2281 cctggagcga tagcgttcgg atggccaggt tccaggcgga acgagttgcc gcggttgtgg
     2341 agacagacgc ctgttccttc ggcgacgata ccactaccga accctttgta taggctgttt
     2401 atgaacgaac agccgttgcc ctccgcgtcg acggcgcaga tgtaggtagt atcacccccg
     2461 gggtcgggag gagagatcac cgctgcggcg tcgctagaga tcgacccgcg cagttttgcc
     2521 gcgtattctt tggagagcaa ctcctccgtc tgtatctcgg agaattcagg gtctccgatc
     2581 ctcccgtgca gatcctcgaa ggcgagcttc ttcgcctcga cctcgaggtg tatgcgctcc
     2641 gcgcccgagg gttccataga ggccaggtcg aacccctcga gaatgtttag catttcgagt
     2701 gcagcgacgc cctggccagg aggcggcatc tcatagaccc ggacacccct gtagtcggtc
     2761 gagatcggct cgacgaaggt cgattcgtgg gctgcgaggt cctcctcaga gaggtaacca
     2821 ccggcctcct gcgtggatcg cgcgatgctc gccgctatct cgccctcgta gaaagcgtcg
     2881 gcgccacgtt cagcgacagc cgagatggtg gccgcaaggt ctacctggga gaagatctcc
     2941 cccgcaacag gagccctgcc ttccggcaga aaggtcctgg tggcggcatc attcgtgcgg
     3001 agtacctcct cgtgctcatc ccaatagcgg gcgatgacct ccccgacggg gtagccattc
     3061 tccgcgagct cccaggctgg ctcgagcagc cgcgccagcg gctggttccc aaagcggtac
     3121 acggcgtctt cccatagcct gacagcgcct gggaccgtaa ccgtgaggcc gccacgctcc
     3181 ggcaactcgc ccagatgaga gagtttcgcg acggtgaggt ccctcgaggc ccggcctgag
     3241 ccattcactc cggttacccg tcctgtctcg gcctcgtaga acagcaggaa ggcatcgcca
     3301 cccatgccag tctgcatcgg cgagagaacc gccatcgcag cggccatggc gacgcacgcg
     3361 tcggcggctg ttcctccacc atctaggacg cggagaccga taatggcggc gcccgggtgc
     3421 tcgcaagcga ccgccccctt cgtcccgaag accgtcgagc ggcctcccag atcaggcctc
     3481 ctcgtggcgc cttccagtcg ttgggacacg tgctgcggac ctcctgtagg gatctattga
     3541 tcgctcatac agtctataga aaggagcggc gagtatattg gcgcttgtaa accaagagga
     3601 ggtaactcat gatcggcggc aaaggaatct acgtgggtgg gaccctgatc tccatcctca
     3661 tctcactggc cctgtacttc tcaggcaggc gcgagtcggc gatcttcgtt ggtctatggg
     3721 cccccacgat aatgaacctg ggccagagcc tgcaagagga ggactagggt gattcgctga
     3781 aatgctgatc tagagtctgc cgaactcgcg gagcagaccg acgagagaca ggcgtttcac
     3841 gatcggcctc ccatgggggc agatctcgat gtgccctgcg ttatggaaga actcccctcc
     3901 aacccatcta ctggcacgcc ggccatttct atctctaaat ccccgctctt ggctaaccag
     3961 accttgagct tgagcatctt gtagaggtgg tagcgctttt ctgagatgag ggagtccagg
     4021 acctccggcg ctagggatgc gtagtgttcg agtactgcgt ctctatcttg ctccatctgt
     4081 tccacgcgtt ccttgtgccg tcgcagatcc ttgagctccc actctgccgt ctgccgcgtt
     4141 tcctccagag ccgctaactc ctcatccagt tcctcgtccc tgatacggcc cttggccgca
     4201 agtcgtagga acccacgtcg ctcctcatcg atctccgaaa gcttctccaa ccagtgtttt
     4261 gcctccgctt cgggatcgcc gtatcgtcct tccctctcca gctcaatggc ctggtccagg
     4321 tcagagcgga gctctgcggg gtccaagagg ataccgcgca cgaattccca cacctggccc
     4381 tctagcttcg tcgcactggc aaagcgggcc ttctcacaag ccttgaaccc gtgcttgttg
     4441 taccctgagc agatgtagta gaagtattgg tagcgccgtc cgcccttctt cttactgcgc
     4501 tttcggctga tcaggacacg attgcaggca gcgcagcggc caatcccacc cgataactcc
     4561 caaaagcgat gcccggctga ggaaggtctc cgattgtcct taatctgctc tcgcgccacg
     4621 tccacgatct cgcgtggcac cccagcatca ggtacgggca cggctatcca ctcttccttc
     4681 ggcttggtgt aggaggaata gcgcttaccg tagcgcctgc cgtcgccaga ggcttcggat
     4741 accagcgtgc tggtcatgcg ccggcggttg aaccaccaca gtccgtactc cttgctaggg
     4801 tcgaggcggg cggcgacctg cggggagacc accgcctcga cctcatcgaa ggagtgcgga
     4861 cggtaaacgt catcgaagac gcagtggcga gagaacgctc tatcccagaa tttcgctccc
     4921 cgtggggttg gcacgccttc ccgttctaat ttgagggcga tagctcgcaa tgtggtgccg
     4981 tccgcgacct cgcggaagat tcgacgcacc actgccatct tctcttcgtc tacctcataa
     5041 gcatcgcgcg cagcattcaa cttgaaaccg tagcgcggcg agcgggtggc tacgaccttc
     5101 ccctctctcg ctttcctgag cctgccccgc cgtgttcgct ctgcggtctt ggcgcgctcg
     5161 aacttcgcaa gctgatcgag aatgccatct gtgagctccc cctccgggct gtcgtcgccc
     5221 ctatcgttga gagatcggat cttcgtaccg tgctcctcga actcacgccg gaggaggtaa
     5281 tggtaagcag gctcgcgggc tatgcggtcc cgatcctggg ccagcaccac ggaaacgccc
     5341 ccgcctgcga cgcttcttag ctcgctacgg ggaggctttg gccgtaggag acctgaaggc
     5401 catctccgag acgctctgat cttgttgcac gctcttctcc tccccaaggg gcccccaagg
     5461 ggctgatccc accgtgcctt tcgaggggcg tcgcttctgg cctaagctcc ttggggctgc
     5521 cccggctcac gtacggcgcc aagctgttgc atcatgccca acaggtcgga gacgctccac
     5581 gactcggcga tctgccctcc ctctatgcga tatatagaga tgcccctaaa gttcacccta
     5641 ttgccggtag gagggatgcc catgtactcc ccttggtggg ttgccttagc cctccagtgg
     5701 gctaccacca tgtccctctc gg
//
LOCUS       DASTKM010000319         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_664525, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000319 DASTKM010000000
VERSION     DASTKM010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664525"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFQ10_12610"
     CDS             <1..600
                     /locus_tag="VFQ10_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966229.1"
                     /translation="RRSGPQTDEDEGTVNALNRLILLIVALLLVAVPVLLLLMNWGII
                     QADLVDQYTGYRGAVQALGDFQASTLTTGARVVIAVVGALVALIALLLLLRELTLGRR
                     VSRSTVMDDTPGRETIITANAVKTLAESAAREVGAESPSASLASDGNSYVVFTGIQVP
                     ASDNYTDLAARARENIQRVLGDQGVPLKDVEVTVRETAL"
     gene            618..818
                     /locus_tag="VFQ10_12615"
     CDS             618..818
                     /locus_tag="VFQ10_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET9966230.1"
                     /translation="MATWTNKQWGAVIGALVLFSMFLIGFEETLVVVLFGIIGYLIGK
                     YLDGEIDTDDIRARAQGRQRGT"
     gene            861..1655
                     /gene="otsB"
                     /locus_tag="VFQ10_12620"
     CDS             861..1655
                     /gene="otsB"
                     /locus_tag="VFQ10_12620"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET9966231.1"
                     /translation="MDGREACERLRTWGEEPGSVALLTDIDGTLAPIVPTPDMSEISD
                     EIRDSLERLSRECLLVAAISGRKTEDALGLLGLEDVVYFGNHGFEILRDGEVEMIPEA
                     RPYLERVEELEQLAREELAAEGAFVEEKGITASVHYRNVPQEVGERSVAFVKREGERL
                     GLRITVGRGVVEARPPIRADKGTAVRTLVEEYRPEKAMFMGDDTTDLDAFRELERLLE
                     EGRLREIMRVGVASEEGPQEIRIEADIVVDGVEGVGRVLRALLGQN"
     gene            complement(1652..>2055)
                     /locus_tag="VFQ10_12625"
     CDS             complement(1652..>2055)
                     /locus_tag="VFQ10_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966232.1"
                     /translation="LYSLIGNNTFSNLFDFHLDPWYLSAMYVLCAVFVGLLVAGGLIA
                     FAEKVLRETFFARYGLMVLVICIGGAALAILLTTVTFLFDNAAYVPRGAAETSYTLVL
                     ATIAGATLGAIEGVILAFPLAWLLGQIGERN"
BASE COUNT          430 a          597 c          691 g          337 t
ORIGIN      
        1 cgtcgaagcg gaccccagac agacgaagac gagggtacag tgaacgccct aaaccgcctc
       61 atccttctga tagtggccct gctgctcgtg gccgtccccg tattgctact gctcatgaac
      121 tggggaatta tccaggccga cttagtggac cagtacacag ggtacagggg cgcggtccag
      181 gcccttggcg acttccaagc ctccaccctc actacggggg cgcgggtcgt gatcgccgtc
      241 gtcggcgccc tcgtggccct gatagcgctg ctgctgctgc tcagggaact cacgctcggg
      301 cgcagggtct cgaggagcac cgttatggac gacacgccgg gcagggagac catcataacg
      361 gccaacgctg tcaagaccct cgccgagagc gcagccaggg aggttggcgc cgaatccccc
      421 tccgcctcgc tcgcctccga cggcaactct tacgtcgtgt tcacagggat acaggtccct
      481 gcttctgaca actacaccga tctcgcggcg cgcgccaggg agaacatcca gagggtgctg
      541 ggagaccagg gtgtgcccct aaaggacgtc gaagtcacgg taagggaaac tgctctttag
      601 agaaaggaag gtaggagatg gccacctgga ccaacaaaca gtggggcgct gtgatcgggg
      661 cactggtgct gttctcgatg ttcttgatcg gtttcgagga gaccctcgtc gtcgtactct
      721 tcggcataat cggctatcta atcggcaagt atctcgacgg agagatcgac accgatgaca
      781 tccgcgctcg cgcccagggc cgccagagag gtacgtaaat ggaatgagcc gctaccgaac
      841 gtatcctgtt agcggtcatg gtggacggta gagaagcctg cgagcgtctg aggacctggg
      901 gcgaggagcc tggctcggta gccttgctca cggacataga cgggacgctc gccccaatcg
      961 tgcctacacc ggacatgtcc gagatctctg atgagatcag ggactcgctc gagcgactga
     1021 gcagggagtg tctcctggtc gcggccatca gcgggaggaa gacggaggac gcgctcgggc
     1081 tcctagggct cgaagacgtc gtctacttcg gcaaccacgg cttcgagatc ctgcgcgacg
     1141 gcgaggtaga gatgatcccc gaggcccgcc cctacctgga gagggtcgag gagctggaac
     1201 agcttgcgag ggaggaactc gccgcagagg gcgccttcgt cgaggagaag ggcataacgg
     1261 cctcggtgca ctaccgcaac gtgccgcagg aggtcggtga gcgctcagta gcgttcgtga
     1321 agcgcgaagg ggaacgcctg ggactcagga tcacggtagg ccgcggtgtc gtggaggcgc
     1381 gtcctcccat ccgggcggac aaaggcaccg ccgtccgcac cctggtcgag gagtatcgcc
     1441 cggagaaggc catgttcatg ggcgatgaca ccacagatct cgacgcattt cgcgagctgg
     1501 agcggctgct ggaggaggga aggctgaggg agatcatgcg agtcggcgtg gcgagcgaag
     1561 agggaccgca ggagatcaga atcgaggccg acatcgtcgt cgacggcgtc gagggcgtgg
     1621 gcagggtcct tagggcgctt ctcggccaga actagttccg ttcgccgatc tgaccgagga
     1681 gccaggccag cgggaaagcg aggatcaccc cttctatggc ccccaacgtc gcccctgcta
     1741 tggtggccag aacgagcgtg taagaggttt cggcggcccc tctcggcacg taggccgcgt
     1801 tgtcgaagag gaacgtgacg gtggtcagga ggatggccag cgctgctccc ccgatgcaaa
     1861 tcaccagcac catcaaaccg taacgcgcga agaaggtctc cctgagtacc ttctccgcga
     1921 acgcgatgag gccgcccgcg accaggagcc ccacgaagac ggcacagagc acgtacatcg
     1981 ctgagaggta ccaagggtcg aggtggaaat cgaagaggtt ggaaaaggtg ttgttgccga
     2041 tcaaactgta gagga
//
LOCUS       DASTKM010000320         9748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_314072, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000320 DASTKM010000000
VERSION     DASTKM010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9748
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314072"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFQ10_12630"
     CDS             <1..529
                     /locus_tag="VFQ10_12630"
                     /inference="COORDINATES: protein motif:HMM:NF013555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HET9966233.1"
                     /translation="FASEIASYEMTAELERSMDEISEGKVSKDSVVDSSRDVLRRVYD
                     HLQNSETEFADIVWEGIRTDETIGKCPESGHDLVIRRSRKSRKRFVGCTGYPDCRVTY
                     PLPQRGEIIPLGTRCDACGSPEIKVLGGKRPWVTCINMECPKKQEQREAAEKAKEGGE
                     GSSTQSAQELQKSTT"
     gene            691..1455
                     /locus_tag="VFQ10_12635"
     CDS             691..1455
                     /locus_tag="VFQ10_12635"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET9966234.1"
                     /translation="MPPIIGITATLKEDVEATAERPLGRFVRTDLDYVEGVAGAGGAP
                     VVLPPTGDEGAAEAVIHSLDGLLLSGGSDLDPGYYGEEPVPELGVTLPERDAFEMALV
                     RLALRRGMPVFGICRGMQVLNVALGGTLYQDLPSQWEWDVLKHRQDTPKWQPTHEVRV
                     REGSYIAEVMGREVVKVNSYHHQGVRVLAEGLLATGFSSDGVIEAVEARDLSERWLLG
                     VQWHAEAMRGAGPQQESLFEAHVSAAERHALREAAA"
     gene            1350..2120
                     /gene="tmk"
                     /locus_tag="VFQ10_12640"
     CDS             1350..2120
                     /gene="tmk"
                     /locus_tag="VFQ10_12640"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET9966235.1"
                     /translation="MARRGDARRRSSAGEPLRGARLGGGAARSARGCGLAGRPAAQST
                     RRGIFVTVEGPDFSGKSTLVRAIRPLLEGSSPSTHFTREPGGTPAAEIIREVVLDPGT
                     KMDAWTEAYLYAAGRADHVRGTILPRLEAGENVLCERYLDSSLAYQGFGRGLGVEPVR
                     ALNAYAVGTVVPDRTFYLRLSQEERERRARELGMPPDRIEAVGASFMRRVEQGFEELA
                     RLEPERIEVLDASLSAEDLARRVVGRMRDLQYTREDDG"
     gene            2113..3225
                     /locus_tag="VFQ10_12645"
     CDS             2113..3225
                     /locus_tag="VFQ10_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HET9966236.1"
                     /translation="MADRRDRFEGVLGNETALRILGRALETGEVSQAYLFHGPPGVGK
                     RMVALRFGAALVAGGVVAAEDRALRGLHPDLTEIVPQGAFTTIGQVREIVRLAASRPF
                     EGARRAFILRADTLNVQAANALLKTLEEPEGETVFMLLASSREEVLPTILSRAQILRF
                     NPVPTGVVAGFLQERGAAEPGLAAQLGRGSVGLSLRYAEDPEFAKLREAVFLAGFALS
                     EDFEGRHEAVRKIVGRAEALGEARERGFMAEFEEPDRRAKEEAKRAGKAARDGALREA
                     LELLAILYRDAAVIESGAEELAANTDRVGEIRGYVEEYPGADWAGTARALGEARAALA
                     YNVSPEAILEVTLSRIRRKILAPSRGSWMSGFPAGA"
     gene            3195..4055
                     /gene="ricT"
                     /locus_tag="VFQ10_12650"
     CDS             3195..4055
                     /gene="ricT"
                     /locus_tag="VFQ10_12650"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HET9966237.1"
                     /translation="MDVRVPGWSITRLCYARDLDLRVGYSVVVATEVGELVGRVALTP
                     RRREPYTQPYDIVRIVTEEDARTEDRNRELGRAVREEARKFARDHTIEGVQFIGCDVS
                     LDGSYIEVKYQTDDKEVDLGPVKSGLERAYDAQIAFREFRFIERSGDASGCDTCGLPL
                     CCATWSGARSMGPVNVRLAKQQGVTPNEKILGCCGEVKCCMRYEHDTYKEFKQRAPFR
                     NSTVKLGDREGKVVDYSMVRDSVFVQFGPRRTDQELISLGSLARDNPGIIPADTDEWE
                     VPEPPQSTDS"
     gene            4063..5025
                     /locus_tag="VFQ10_12655"
     CDS             4063..5025
                     /locus_tag="VFQ10_12655"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET9966238.1"
                     /translation="MLDNLCDVPGVLVGHATHSEGVTGCTAVLFEGQEGAVVGVDVRG
                     SSPGTRETDRLGPVGAVRPTHALLLTGGSAFGLAAVDGVVRFLEESGIGLDMGVARIP
                     LVSAAVLFDVVVGDPSVRPDAAMGYEAASSATSGDFAQGSVGAGTGATVGKVLGMDRA
                     MKGGLGSASAHLAGGLVVGALAAVNAFGEVRDPRTREVLAGPRLEDGTLGDTVELLPE
                     AAARLQWGESTTLGIVATNARLSKPQVSKVAQMGHDGLARAVYPVHTSVDGDVVFAAS
                     VGAVEEAPDVVGAWGARVMEDAIVRAVLAAVGVAGLPAASDLTR"
     gene            complement(5079..6146)
                     /locus_tag="VFQ10_12660"
     CDS             complement(5079..6146)
                     /locus_tag="VFQ10_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9966239.1"
                     /translation="MIAARLHRYDDSIPKDSLKVEEIDEPRIENPLDVIVRIGAAGLC
                     RTDIHVIEGQWQPIQDPDNRLLPYILGHENSGWIEEVGSAVTNVEVGDTVICHPLMTC
                     GLCRACRAGDDMHCEKGEFPGISRDGGFADLLKTNARAVVKLDPILEPKDIAALADAG
                     LTAYHAVRKSVPLLYAGTKALVIGAGGLGHIGIQCLKALTPTEIIVTDPSEEALELAK
                     EWGADQIVRVEDGYVEKVLEITDGNGAEVVFDYVGERGAQDDAWAMTRQGGSHYVIGY
                     GGMVEVPAIDIISTERNITGNLVGTYNDLVELMVLTAQGKVNLHTSTYPLDAVNDAIQ
                     ALNNGELRGRGILIPEGANAA"
     gene            complement(6143..6841)
                     /locus_tag="VFQ10_12665"
     CDS             complement(6143..6841)
                     /locus_tag="VFQ10_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HET9966240.1"
                     /translation="MMLNRATVLDALSGVRDPELDEPITDLQFVSSLDIEDGAVSVNL
                     RLPTYYCAPNFAYLMVADAREALLSVEGVEEARVVLDDHYASREINGGVNGEFGFARS
                     FPGQTEGDTLDELRNIFRRKSFVSRQEQLCRSLIREGRSPEELAGMRLREAREDGTFE
                     KYLERRSELGIDTSPEAPLVVDPDGKQIPREAVVEHLRFASTVRRSIEGNAELCTGLL
                     AVRYGNPSREEATA"
     gene            complement(6838..7845)
                     /locus_tag="VFQ10_12670"
     CDS             complement(6838..7845)
                     /locus_tag="VFQ10_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9966241.1"
                     /translation="MYEKDGEKYFVVDGHVHYWNASPENWIEGQEEYAKGWIECFYAY
                     HQLGPEEYYWPIEKFQKYSEETMMKDLFEDGYVDKAIFLPTNLREWYKDGFNTTEKNA
                     TLAEKYPDKFILNTTFDPREGSEGLKEFEEKVNRYGCKGVKLYTAEWTGDSKGWTLAD
                     PEAEPYFEKCMDLGVTNIHVHKGPTIWPLTKDAFDVKDVDVAATHFPKLNFIVEHVGL
                     PRIEDFCFMATQEPNVYAGLSVALGALMHSRPRFFATVMGELLFWVGEDKMLWASDYA
                     IWHPKWQAEMFVDWDYPEGHEYDDFPRLSTEGKKKILGLNAARLYDIEVPAELQVAAT
                     A"
     gene            8281..8436
                     /locus_tag="VFQ10_12675"
     CDS             8281..8436
                     /locus_tag="VFQ10_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966242.1"
                     /translation="MASFKEQGATLEPKTLTADRHDYKISFIEDPHGYKLELVQRETM
                     KVEEIIP"
     gene            complement(8466..8681)
                     /locus_tag="VFQ10_12680"
     CDS             complement(8466..8681)
                     /locus_tag="VFQ10_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966243.1"
                     /translation="MEKVRSRAPETSPSQEAVVLVLDNCYVSCPAGPRRGLPAAERAE
                     RKDFSENLYLLSGMDVGETRNRLNGAA"
     gene            8814..8886
                     /locus_tag="VFQ10_12685"
     tRNA            8814..8886
                     /locus_tag="VFQ10_12685"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8847..8849,aa:Thr,seq:cgt)
     gene            9460..>9748
                     /locus_tag="VFQ10_12690"
     CDS             9460..>9748
                     /locus_tag="VFQ10_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9966244.1"
                     /translation="MTDLKKAAIFTEVSLMLAAVFWGTNYAATKYAAEFTPPLLIVAL
                     RFTAGGILMFCLLRILEPADRLKPKDLLSVAGLGCLGVAIGQTAFTFGVSMT"
BASE COUNT         1754 a         2965 c         3169 g         1860 t
ORIGIN      
        1 cttcgcctcc gagatcgcct cgtacgagat gaccgccgaa ctggagcgga gcatggatga
       61 gatctcagag ggcaaggtca gcaaggattc cgtcgtcgac tcctcgcgcg acgtgcttcg
      121 cagggtctac gaccatctcc agaactccga gaccgagttc gcggacatcg tgtgggaggg
      181 cataaggacc gacgagacca taggcaagtg ccccgagagt gggcacgatc tggtcatccg
      241 ccgcagccgt aagagccgca agcgctttgt gggctgcacg ggctaccccg actgccgggt
      301 cacctacccg ctcccgcagc ggggtgagat catacccctc ggtacccggt gcgacgcctg
      361 cggctctcca gagataaagg tgcttggggg gaagcgtccc tgggtaacat gtattaatat
      421 ggagtgccct aagaaacagg agcagcgtga ggccgccgag aaggcgaaag aggggggtga
      481 gggctcatcg acacagagcg ctcaggaact tcagaagtcc accacataga gagcaggctc
      541 gacctggacc tggtcgatga gccccctttc tataaaccta ctacgtatac ccgcgctacc
      601 ggctacctca ttctcctcgc catcatcgtt gtggtctggt attctgccgt atatagttga
      661 gttaagatat tgttaaaagg tgagtagaga ttgcccccga ttattggcat aacggcgacg
      721 ttgaaagagg atgtggaggc gactgcggag cgtccgttgg gcaggttcgt gaggaccgac
      781 ctcgactatg tggagggcgt cgctggggct gggggcgcgc ccgtagtctt accgcctacg
      841 ggggatgagg gggctgctga ggcggtgatc cacagcctcg acgggttgct cttgagcggc
      901 gggagcgacc tggatcccgg ctattacggc gaggagccgg tacccgaact cggggtaacg
      961 ctccccgagc gggatgcatt cgagatggcg ttggtgaggt tagccttgcg gcgtgggatg
     1021 ccggtcttcg gtatctgccg ggggatgcag gtcctgaacg tagcccttgg cggtacgttg
     1081 taccaggacc ttccttcaca gtgggagtgg gacgtgctca agcacaggca ggacaccccc
     1141 aagtggcagc ccacgcacga ggtaagggtc agggaagggt cctacattgc cgaggtcatg
     1201 ggccgcgagg tggtaaaggt caactcttac caccatcaag gggtaagagt cctcgccgaa
     1261 gggctgctcg cgacgggctt ctcttcggac ggtgtgatcg aggctgtcga ggccagggat
     1321 ctctccgagc ggtggctgct cggcgtccag tggcacgccg aggcgatgcg cggcgccggt
     1381 cctcagcagg agagcctctt cgaggcgcac gtctcggcgg cggagcggca cgctctgcgc
     1441 gaggctgcgg cttagctgga aggccagctg cgcagagtac acggaggggc atcttcgtaa
     1501 cggtcgaggg ccctgacttc tcgggcaagt ccaccttggt acgcgccatc agaccactgc
     1561 tcgaaggttc gagtccttcg acacacttca cccgcgagcc cggcggcacc ccggcggccg
     1621 agatcatcag ggaggtcgtc ctcgaccctg ggacaaagat ggacgcctgg acggaggcgt
     1681 acctgtacgc tgcaggccgc gctgaccacg tgcgcggtac gatactcccg cgtctcgaag
     1741 ccggtgagaa cgtgctctgc gagcggtacc tggactccag cctcgcctac cagggcttcg
     1801 ggagagggct cggcgtcgag ccggtacgcg ccctgaacgc ctacgccgtc gggaccgtgg
     1861 tgcctgacag gactttctat ctgcgactct ctcaggagga gagggaacgg cgggccaggg
     1921 aactcgggat gcccccagac aggatcgagg cggtaggcgc cagcttcatg cggcgtgtgg
     1981 agcagggttt cgaggagctg gcgcgtctgg agcccgagag gatcgaagtc ctcgacgcct
     2041 cgctctcggc ggaggacctc gccagacgtg tggtgggtag gatgcgcgac ctgcaatata
     2101 ctcgtgagga tgatggctga ccggcgggac aggttcgagg gcgtactcgg gaacgagacg
     2161 gcgctgcgga tcctcgggcg ggctctagag accggcgaag tctcgcaggc gtatctattc
     2221 cacggtcctc ccggcgtggg caagcgaatg gtggcgctga ggtttggggc cgcgctggtc
     2281 gccggaggcg tcgtggcggc ggaagacagg gcgctcaggg gactgcatcc tgacctgacg
     2341 gagatagtac cccagggggc gttcacgacc atcgggcagg tgagggagat tgtgcgtctg
     2401 gcggcgagcc ggcctttcga gggtgcacgc cgggcattca tactgagggc tgatacgcta
     2461 aacgtgcagg ccgcgaacgc cctactcaag acactcgaag aacccgaagg tgagacggtt
     2521 ttcatgctgc tcgcgagttc gcgtgaggag gtactgccca cgatcctgtc ccgcgcccag
     2581 atcctacggt tcaaccccgt gccgacgggc gtcgtcgcgg ggttcctgca agagaggggc
     2641 gctgctgagc ccgggctcgc tgcccagctc ggcaggggaa gcgtcgggct ctcactgcgc
     2701 tacgcggagg accccgagtt cgcgaaactg cgcgaggcag tctttttggc gggattcgct
     2761 ctctcggagg atttcgaggg aaggcacgag gccgtcagga agatcgtcgg gcgtgccgaa
     2821 gcgttggggg aggcgcggga gcgcgggttt atggcggagt tcgaggagcc ggacaggcgc
     2881 gcgaaagaag aggccaaacg tgcgggaaag gccgcgaggg acggtgccct gagagaggct
     2941 ctggagttgt tggccatcct gtatagggat gctgcggtga tcgaatcagg tgcggaggag
     3001 ttggccgcca acacggacag agtgggggag ataagggggt acgtggagga gtatcctggg
     3061 gcggattggg ccgggaccgc acgtgctctt ggggaagcac gcgcggcact tgcgtataat
     3121 gtctccccgg aggcgatcct ggaggtgact ttatcgcgca tacggcggaa gatactcgca
     3181 ccctcccgag gctcgtggat gtcagggttc ccggctggag cataaccagg ctctgctacg
     3241 cgcgggacct ggacctcagg gttggctact cggtagtcgt cgcgaccgaa gtgggggaat
     3301 tggtcggccg cgtcgccctg acccctcgtc gccgcgagcc gtacacacaa ccttacgaca
     3361 tagtcaggat cgtcactgag gaggacgcca ggacggagga tcgcaacagg gaactgggac
     3421 gcgccgtcag ggaagaggcc aggaagttcg ccagggacca cacgatcgaa ggcgtgcagt
     3481 ttatcggttg cgacgtctcc ctggacggct cctacatcga ggtcaaatac cagaccgacg
     3541 acaaggaagt ggatctgggg cccgtcaaga gcggcctcga acgggcctac gacgcgcaga
     3601 tcgccttcag ggagttccgc ttcatagagc gctcggggga cgcaagcgga tgcgacacgt
     3661 gcggcctgcc cctgtgttgc gcgacgtgga gcggtgccag gagcatgggt cctgtcaacg
     3721 tcaggctggc caagcagcag ggcgtgacgc ctaatgagaa gatcctgggc tgttgcgggg
     3781 aggtgaagtg ctgcatgcgc tacgagcacg acacctacaa ggagttcaag cagcgcgccc
     3841 ccttccgcaa ctccaccgta aagctcggcg acagggaagg taaagtcgtc gactactcca
     3901 tggtcagaga ctccgtcttc gtccagttcg gcccgaggcg cacggatcaa gaactcatct
     3961 ccctcggctc cctggccagg gacaacccgg gcatcatacc tgccgacaca gacgagtggg
     4021 aggtgccgga acctccccaa agtacggact cctagcgccg gcgtgctgga taatctctgc
     4081 gacgtgcctg gtgtgctcgt cggccacgcg acacacagcg aaggggtcac aggctgcacc
     4141 gctgttctct tcgaaggcca ggaaggagcc gtcgtcggcg tggacgtacg gggctcctct
     4201 cctggcacgc gagagaccga ccgcctcgga ccggtaggcg ccgtccgtcc cacccacgcc
     4261 ctgctcctga ccgggggtag cgccttcggg ctcgccgccg tagacggcgt cgtccgattc
     4321 ctcgaagaga gtggcatcgg tctcgacatg ggcgtggccc gcatacccct ggtctcggcg
     4381 gccgttctct tcgacgtcgt cgtaggagat ccctcggtac gtcccgacgc ggcgatgggt
     4441 tacgaggcgg cctcgtcggc cacgagcggc gacttcgccc agggaagcgt aggagcaggc
     4501 acaggagcga cggtagggaa ggtcctgggt atggatcgcg cgatgaaagg tgggctgggg
     4561 agcgcctcgg cccacctcgc cggcggcctt gtcgtgggcg ccctcgccgc cgtgaatgcc
     4621 tttggtgagg tcagggaccc gcggaccagg gaagtgctag caggtcctcg actggaagac
     4681 ggtacgctgg gcgatactgt ggagctactc cctgaagccg ctgcccgcct gcagtggggg
     4741 gagagcacaa ccctcgggat cgtggccacc aacgcgcgcc tctccaagcc gcaggtaagt
     4801 aaagtcgccc agatgggcca cgacggcctc gcccgcgccg tatacccggt ccatacgtcc
     4861 gtagacggtg acgtcgtctt cgccgcttca gtgggtgcgg tggaggaggc gcctgacgtc
     4921 gttggtgcgt ggggggcgcg ggtcatggag gatgccattg tgcgagccgt cctcgccgcg
     4981 gtgggcgtcg caggcctgcc cgctgcgagt gaccttacac gctaacgctg ccccttcagc
     5041 gggtagggga tgggccacct acccgcgctg gtctacactc aggcggcgtt cgcgccttcg
     5101 gggatcagga ttcccctccc cctgagctcg ccgttgttga gggcctgtat cgcgtcgttt
     5161 acggcgtcga gcggataggt cgaggtgtgg aggttgacct tgccctgggc ggtgagcacc
     5221 atcagttcga cgaggtcgtt gtaggtgccg accaggttgc cggtgatatt gcgctcggtc
     5281 gagatgatat ctatggccgg gacctccacc atgccgccgt atccgatgac gtagtgcgat
     5341 ccgccttgtc tggtcatcgc ccaggcatcg tcctgcgcgc cgcgctcgcc gacgtagtcg
     5401 aagaccacct cggccccatt gccatcggtt atctccagta ccttctctac gtagccatct
     5461 tcgactctca ctatctggtc tgcgccccac tctttggcga gctccaacgc ctcctccgag
     5521 ggatcggtga cgatgatctc cgttggtgtc aacgctttga ggcactggat gcctatgtga
     5581 ccgagccccc cggccccgat gaccagggcc ttcgtacccg cgtagaggag cgggacggac
     5641 ttcctaacag cgtggtaagc ggttaggccc gcgtcagcaa gggcagctat gtctttcggc
     5701 tccaggatgg gatccagctt gacgaccgcg cgggcgttgg tcttcaagag atcggcgaag
     5761 ccgccgtcgc gggaaatgcc ggggaactcg cccttctcgc agtgcatatc gtccccggcg
     5821 cggcacgccc ggcacaggcc gcaggtcata agcgggtggc agatcaccgt gtccccgacc
     5881 tcgacgttgg tcaccgccga gccaacctcc tcgatccagc ctgagttctc gtgtccgagg
     5941 atgtagggca gcaacctgtt gtccggatcc tgtatcggct gccactgacc ctcgatcacg
     6001 tgtatatcgg tgcggcacag tccggccgca ccgatcctga cgatcacgtc gaggggattc
     6061 tctatcctgg gctcgtctat ctcctcgacc ttgaggctgt ctttggggat gctgtcgtcg
     6121 tagcggtgca acctcgctgc tatcatgccg tcgcctcctc gcggctgggg tttccgtaac
     6181 gaaccgccag gagccccgtg cagagctccg cgtttccctc gatgctcctc cgcaccgtgc
     6241 tggcgaaccg caagtgctcc acgaccgcct cgcggggtat ctgcttgcca tcggggtcca
     6301 cgacgagcgg ggcttccggg gaggtgtcta tgcccagctc cgagcggcgc tccaggtact
     6361 tctcgaacgt accatcctct cgcgcttcgc ggagacgcat accggccagt tcctcaggcg
     6421 agcgcccctc gcggatcagg gacctgcaga gttgctcctg ccggctaacg aaggacttgc
     6481 ggcggaagat gttgcgcagt tcatcgaggg tgtctccttc agtctggccg gggaaggagc
     6541 gtgcgaagcc gaactccccg ttgacgccgc cgttgatctc acgcgaagcg tagtggtcgt
     6601 ccaggacgac cctggcctcc tcgacgccct ccaccgagag cagagcctcc ctggcgtcgg
     6661 cgaccattag gtaggcgaag tttggggcgc agtagtaggt cgggagcctc aggttgacgc
     6721 tcaccgcgcc gtcctctata tccagagatg agacgaactg caagtccgtt atgggctcgt
     6781 cgagttcggg gtcccggacg cccgagaggg cgtccaggac cgttgctctg ttcagcatca
     6841 tgccgtcgcg gcgacctgca actccgcggg cacctctatg tcgtagagcc tggcggcgtt
     6901 gaggccgagg atcttcttct tgccctcggt cgagagacgg gggaagtcgt cgtactcgtg
     6961 cccttcgggg tagtcccaat caacgaacat ctcggcctgc cacttcgggt gccagatcgc
     7021 gtaatcgctg gcccacagca tcttgtcctc gccaacccag aacagcaact cgcccatgac
     7081 cgtcgcgaag aagcgcgggc ggctgtgcat cagcgcgcca agtgcgacgg agaggcctgc
     7141 gtacacattc ggctcctgcg tcgccataaa gcagaagtct tctatgcggg gcaacccgac
     7201 atgctcgacg atgaagttga gtttggggaa gtgcgtcgcg gcgacatcca cgtccttgac
     7261 gtcgaaagcg tccttcgtga gaggccagat cgtcggtccc ttgtggacgt ggatgttggt
     7321 gacgccgaga tccatgcact tctcgaagta cggttctgcc tctggatcgg cgagcgtcca
     7381 gcccttcgag tcacccgtcc attccgccgt atagagctta acgcccttgc agccgtagcg
     7441 gttgaccttc tcctcgaatt ccttgaggcc ctcgctaccc tcgcggggat cgaaggtggt
     7501 gttcaggatg aacttgtctg ggtacttctc cgccagcgtc gcgttcttct cggtggtgtt
     7561 gaaaccgtcc ttgtaccatt ctctgaggtt ggttggcagg aagattgcct tgtcgacgta
     7621 cccatcctcg aagaggtcct tcatcatcgt ctcttcagag tacttctgga acttctctat
     7681 cggccagtag tactcttccg gaccgagctg gtggtaggcg tagaagcact cgatccaacc
     7741 cttcgcgtac tcctcctgac cctcgatcca gttctccgga gatgcattcc agtagtggac
     7801 gtgcccatcc accacgaaat acttctcgcc atccttctcg tacaaagcgc tctcctcctt
     7861 ttcccacacg tggtacgccc gcgtcaagct cccggacgta tcgccctatc ttgcgttccc
     7921 aagcctcaag caggacctgt cttgaggata agtggaacgc cccagcggaa tccgatcaaa
     7981 ggctttgtgg ctccttggtg ttacctcctt ccccggttag aagccggtcg cgcaagtcgc
     8041 cggccgcccc ttcgtggcgc tgatgataag cgacccggga ttgcggggca accgcggccc
     8101 tcgttttcga ggccctctag taaagtctcg attcaattgg gctcgggggg atctcttcct
     8161 gcggcttcgt gccaggatcg ttggcgtcgg tgccgctcac ggtaataagc gaccgcacct
     8221 tcatgtcatg gtccgcatgg atctggcacg ataggcaagc ttcctccagt agagagcaag
     8281 ttggcgagct tcaaggagca gggcgcgacg ctagaaccca agacgctcac cgccgacagg
     8341 cacgattaca agatctcctt catcgaggac ccgcacggct acaagctaga gctcgtccag
     8401 cgcgagacga tgaaggtgga ggaaatcatc ccgtagcgca ctcccggccg ggagcccgtc
     8461 ctcggctacg cggccccgtt cagcctgttg cgggtctccc ctacgtccat gccggacaga
     8521 aggtacaggt tctcgctaaa gtccttgcgc tccgctcgct cagctgcggg tagccctcga
     8581 cgaggtcctg cggggcagga gacgtagcag ttgtccaaga ctaggactac cgcctcctgg
     8641 ctcggggatg tttccggggc acgactcctc accttctcca cccctctact ctcccggagg
     8701 ccccacgctt cgatccagga acgatggctg aaggctactc ctcggggctc cagcctcaac
     8761 cacacccgct ttaaagcgtg cacccgctac gatatacttt gcgccctgaa caggccgacg
     8821 tagctcagca ggtagagcag ctcactcgta atgagcaggt cagcggttcg agtccgctcg
     8881 tcggctctct attttacctg caaaacctat aaaaagagta gagcccacga tcatgctact
     8941 ggggtcttgg cagcagtagg gcagcagtag actattatcc cggggctccg tccataccct
     9001 cggcggcgtg tcttcccatg ctgggtagcc agtgcgtgta gcgggacgct gctcggatgc
     9061 ctccggggag cgtggtgggt ggtccgcact tcccaacgga ctgtacgaac cggcaacgac
     9121 ctgatgaacc gcttcttggc cacaccggga gaggagctgg aggtcaaggt ggcgctgggc
     9181 aagcggttac gcgttaatgt gctgctcaca gaactgtata caaggtatgt tcttcagagt
     9241 gcacggagaa atctgcgacg aagacctaac cgcgggacac gcactaccat gaatgcctcc
     9301 actacaatgt tgcgcattgg aacggtgctc ccgtcgcatg aagcatagat agcttcttgt
     9361 gatcgaccag cggtagtcga ataattatcc tagttccctt cactcgcgca cttccggcct
     9421 cggtgcgctc gatgcggtcc ggccttataa tctcgtctcg tgacagacct aaagaaggcg
     9481 gccatcttta cggaggtctc tttgatgcta gccgccgtct tctggggcac caactacgcg
     9541 gcgacgaagt acgcggccga gttcacgcct ccactgctca tcgtggcctt acgcttcaca
     9601 gctggcggta tcttgatgtt ctgcctcctc cgcatcctgg agccagcgga caggctcaaa
     9661 ccgaaagacc ttctctccgt ggcgggcctg gggtgcctcg gggtggcgat cggccagaca
     9721 gctttcacct tcggcgtgag catgacca
//
LOCUS       DASTKM010000321        14409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_314302, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000321 DASTKM010000000
VERSION     DASTKM010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14409
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314302"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2099)
                     /locus_tag="VFQ10_12695"
     CDS             complement(<1..2099)
                     /locus_tag="VFQ10_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9966245.1"
                     /translation="MREAGEKSVPLTGTLRVLLVEDSENDAMLLLRELRRGGYEPLSQ
                     RVCTPEDMEKALRSAEARDEPFQVVISDYYLPRFRAPHALRLLRELGYDVPFIVVSGK
                     IGEDAAVEIMKAGANDYLTKENMSRLCPAIERELREVEVRRERETVQKALSLSEDRFR
                     HLVEQIPAVTYVQEPIDSDTPKAITYMSPQYEAMLGYPPEKEILDEEHWLRVLHPDDR
                     ERVLAEELRTDETGEPYRIEYRQIARGGRVVWVHDEATLIRDEEGNALYWLGVQYDIT
                     EQKRTEEELRQSEERFKVLAQEVVEGIILSENGKIIDANRSVTEMFGYGLEELIGRNA
                     IELTSPEYREMVRQRISDEDTRPYESMGLKRDGTTFPIEIRPRHLPYSGRRIRVTSVI
                     DLTERKQAEEALIMSEERYRTLVEQLPAVTYMQETENATLAYVSPQIESVLGYSPEEY
                     LANPDLRWQTIHPEDRGWVLREDIRTDETGEPYSVVYRRISRDGRVVWVREEAMLVRD
                     SEGRPLFWQGILMDVTERKHQEEALRQSEALYRTVVEQAEENIFLVDAKSRRVLDAND
                     ALLRSLGYTHDELKEMTLYDIVAHDQESVDLNIGRIMVEGHRSLGERQYRRKDGSLAD
                     VEVNVSAVPYNGEKVMCVVAHDVTERKRTERMLEEIREAERNRLARELHDSTLQDIVY
                     ALQEVQVMQVVSGDDLNP"
     gene            complement(2092..3978)
                     /locus_tag="VFQ10_12700"
     CDS             complement(2092..3978)
                     /locus_tag="VFQ10_12700"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HET9966246.1"
                     /translation="MEDLIGGFRAHLPSVRLALGRLLRHLRLAVAAYGVLLISLLLAG
                     LAWYYVRQTVEAQDQVRFDETIQATQAAVDRRTDAYLDALFGARGVFLASKAVEREEW
                     ESYVEGIETKSRLEGLQALGFARYVRPAERAAFVSEARREGMPAMRPDLDPGGERSAY
                     FPLALVAPTDEANLSMINQDAYTEQAHKVAMDAARDTGSPQATRMVYVLTGPTANSSA
                     DLALSPGFAVYLPVYAEGEPVENVAARRRALKGFVVGYFRRDGLLDDVFGSGFDPAID
                     FEVYDGADVESSSLLYDEDGVERAGEEGYDPLFSEKSRFGVAGREWSLYFATLPGFKN
                     GAESNLPAFVLASGIGASVLLFGISWMLVRSRILAERASKDLEDANRELEGTNRELEA
                     FSYSVSHDLRAPLRAIDGFSQILQEDYEQALDDEGMDYLGRVRAASGHMATLIDDLLD
                     LSRVGRRPLRREHVDLSSLAAGILEELRASQPGREVEFSAGENIMAWGDASLLKVALE
                     NLLGNAWKFTARKEAARIEFGADRKPGQGLQSPVYYVRDNGAGFDQTYADKLFGAFQR
                     LHGQDEFEGTGIGLATVARIVHRHGGRVWAEGTVGEGATFYFTLGGRDVGDRAVPAKK
                     AEVA"
     gene            complement(4211..4441)
                     /locus_tag="VFQ10_12705"
     CDS             complement(4211..4441)
                     /locus_tag="VFQ10_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966247.1"
                     /translation="MAEGYFELRPVGPYSFGASVRYEGRRSGRLRMAYGLDAPPSAEE
                     LERLGRSWRPHRTWVSLHLRAMLEDEIGEIGR"
     gene            complement(4434..4985)
                     /locus_tag="VFQ10_12710"
     CDS             complement(4434..4985)
                     /locus_tag="VFQ10_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966248.1"
                     /translation="MSQLPPMTYGLLSYLVLACASAVVGYYFARKRTEYEVGYRHRVE
                     VAESVQGMVIPLVEEFDAALEYVRAPGPSGELPIEDIERSVDGLEKYVEQQEMWLDRG
                     SSAALGDLIASLRARQRMLDLLPRRYDDPGFERAYERAEADLDEWLRAELPAAREDLA
                     DAFRVMLGVGRWRRGVLERSTDG"
     gene            complement(4982..5620)
                     /gene="lexA"
                     /locus_tag="VFQ10_12715"
     CDS             complement(4982..5620)
                     /gene="lexA"
                     /locus_tag="VFQ10_12715"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET9966249.1"
                     /translation="MEESLHPRRERILRYLARRAGAGEPPPSVREVGIEVGLRSSQTV
                     HHHLRKLEEEGYVVRDRGARMVRLTEKGWGVAGRIGLLGRIAAGRGLEAVATDEAYSL
                     AAELLASGSGKRRYLLRVVGQSMVGARIEDGDLLVVEEDEIPPDGSVVVVLLREGEEV
                     TVKRFYREGETLRLRPEAPPGEYEEMVVPAEEARVQGRVVYVLHPPGRKGGA"
     gene            5784..6050
                     /locus_tag="VFQ10_12720"
     CDS             5784..6050
                     /locus_tag="VFQ10_12720"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9966250.1"
                     /translation="MSVPLYAASGIPEVWLIDLNSDTIEVYSEPGPGAYEKSARFGRG
                     DQVLSATLPGLAIDANVALPPKKCMSEINPPLTSCRGRRLGEAL"
     gene            complement(5998..6909)
                     /locus_tag="VFQ10_12725"
     CDS             complement(5998..6909)
                     /locus_tag="VFQ10_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9966251.1"
                     /translation="MAILISACQIADTGETDMSVEEMHDDWHSLDLAEEAVIVTSPDG
                     RTAAYADVFNRSFVIVSIYGYVHPDYRGMGLGSYLVAWGERWTRDHMPQAQENARVVA
                     QHYINSANEAARRLLENSGYTPVRGIYVMETKLEEAPPLPHWPADISVRTFVPGWDER
                     AVYEAVEDAFRDMWGRPRGTFERFVGMTKAESFDPSLWFLALEGDEIAGVTLCKTLAG
                     EGWVDVVGVRRPWRNRGLGLALLRHAFTEYHRRGTPRVSLSVDAESVTGAPRLYGRAG
                     MRVRESYVIHLKELRPGVDLGKRSEAD"
     gene            7017..7259
                     /locus_tag="VFQ10_12730"
     CDS             7017..7259
                     /locus_tag="VFQ10_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966252.1"
                     /translation="MNEDAAPITMDFASDKPLIPVANLVTEGSGVSRTLNAMPKQSGT
                     ATLTLSDGEDTGTLLVKVQLAIYGFPPGDEIRPFRT"
     gene            complement(7356..9962)
                     /locus_tag="VFQ10_12735"
     CDS             complement(7356..9962)
                     /locus_tag="VFQ10_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9966253.1"
                     /translation="MTSYLCTQCARQFGMIGGAQGGSDPFAMLQNLIQQQQGGQGSLF
                     DALSGEAQQAVMRAREASAGTTRTAAIGTDHLLAGIVTGDNVATEALQRAGADVGGMR
                     ARFQEFRGDPGEEIYTFTPSAKRALQLSFTAARQMGAGHVGSEHLLLGLLGEGDGNAY
                     RILQQHGANDAEALRREILRLMQQRGAQMGQGMPGAAAGGPGMGGGQQGPPSRTPTLD
                     QVSRDLTQLASDGELDPVIGRAEEISRVVRILSRRTKNNPVLIGEAGVGKTAVAEGLA
                     QRIVSDDIPETLRGKRVLALDVGGLVAGTRFRGDFEERMQQMLKELQQEEKNIILFID
                     ELHTIVGAGGAEGAVNASNMLKPALARGELQVVGATTLDEYRKNIEKDAALERRFQPV
                     LVDEPTVDETVAILFGLRDRYEAHHRVKITEEAIIAAAQLGDRYITDRFLPDKAIDLL
                     DEAAAEVRLRSTVPPVDVKRIEEEIAGLEREKEDAVREEDYEKAAGFKQRIEQLKAEL
                     EEQHEGWAGRRETNAPEVTREDIARILEEWTGVPATNIVKEEAERLLSLEAVLHERVV
                     GQEPAVKAVAEAIRRARAGIKDPKRPVGSFIFLGPTGVGKTELARTLAEYLFGEEDAM
                     IRIDMSEFQESHTVSRLVGAPPGYVGYEEAGQLTEQVRRRPYSVVLFDEIEKAHPDVF
                     NTLLQVLDDGRLTDAKGRTVNFENTVVIMTSNVGSQHLVSTRQFGFTSGDGVDFRETE
                     RRAREALERAFRPEFLNRVDEIIVFQPLTKEDVLKIVDIMLSHLNKHLESQRVQIEVT
                     DAAKEFLAEEGYDPKFGARPLARAIRRYVENPLSSRIIGGEFDPGDTVVVDHKGEGRE
                     ELTFQVKERVSS"
     gene            10225..11196
                     /gene="pfkA"
                     /locus_tag="VFQ10_12740"
     CDS             10225..11196
                     /gene="pfkA"
                     /locus_tag="VFQ10_12740"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET9966254.1"
                     /translation="MRIGILTSGGDAPGMNAAVRAVARTALFRGWEVMGVWAGYRGLL
                     EGRIYPMDRRRLGGIIHRGGTVLGTERSAEFSTPEGQRSAAGKLAEAEVEGLVVIGGN
                     GSLTGGLRLAELGVKVAGIPATIDNDVWGTDTAIGVDTALNTALEAIDRIKDTASSHQ
                     RAHAVEVMGRDSGYLALMSSIAGGAEAAVVPEFEPRPEALLSFLKKAYEQGKSHFIVV
                     VAEGAPLSAEELHEYINESEGTYDSRLTVVGHIQRGGIPTASDRILASRLGTAAVEAL
                     ADGETGVMVGIRGPRIERGPLEEVVSKEQPLDSDLYRMAEVLAELPE"
     gene            11254..11931
                     /locus_tag="VFQ10_12745"
     CDS             11254..11931
                     /locus_tag="VFQ10_12745"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9966255.1"
                     /translation="MQERSLIGLEARAADGSEVGRIADLVEDEESGEVTHVVVETERG
                     EWLEVPITDLSLDPDADFATFEADASDEEPGDHTGDEAEPEDYYAPDDIDDENEEDFE
                     HEGQLVTEPEDGHDPSSEEDLEAREDWEDHSYTPDSGYPRNDVYIDPDTGDEEIDPLL
                     EENDDVKDDVEDLLDSTSLEVRSVREGVVELTGTAATQDDLEETVREIMGLDEVLEVD
                     TTDVEVR"
     gene            complement(12049..>14409)
                     /locus_tag="VFQ10_12750"
     CDS             complement(12049..>14409)
                     /locus_tag="VFQ10_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester reductase domain-containing protein"
                     /protein_id="HET9966256.1"
                     /translation="PSLLEAVLNSSGPDVGERLAKLRTLWLNGEVVTRTLARRAMDLL
                     PDARLLNVYSCSETHEVAAGDLRELVENPEATYCAIGVPMDQEHLYLLDSDGERVPEG
                     EAGELFVGGDCLARGYVDLPEKTEESFPEDPFSSRSGARMYRTGDRARSLSDGSLEIL
                     GRVDFMVKIRGYSVELGAVEAAIEKSLAVRSCVVVSEGEEGEDKRLVAYIVPDPDDEG
                     RYSGWSLDRRTGRSKEIRSVLQDSLPHYAVPSVFVELESLPIQTTSGKVDRAELPPPP
                     PRPARSPRQDTGSVDRLPESAPRREKEALLTRSWEDVLRLDQGEVRPEDDFFDLGGHS
                     LAAAQLSGLVEQGFGVHVSMPLFMEDPTLGGLLERIEELQRDGTSGGVTDAEDLTAEA
                     VLDPGIVPQPAANGTPTLRDTSDVFLTGVTGFLGAFLLDGLLSGTNARVHCLVRPRGD
                     AGGMESIEANLRRYGLWKPERAERILAVRGDLGKPLFGLDEGDFDALAREVGLIFHAG
                     AVVNLVYPYSELKPANVGGTREVLRLACRHGAKLVHHVSTNGIFPPGMGRCEEGADLD
                     ALAGAREDGYGRSKWVAEKLVREAAGRGVPVCVYRPGNVSGHSETGASNPRDLLGAVV
                     VESLRLECAPEIDGWNTEMTPVDFVAAAILHIASDPAAEGETYHVANPEPPPAGEVFD
                     WLEEQGHRLERVPHDQWLQRLDTAPPEEGSPGEVVRAAAPAAEDLRDDNTYDDHNTRR
                     ALSDGGPTRPAIDGTLMQTYARYFAQQGWIVTSGIGKQVSAGSRRG"
BASE COUNT         2467 a         4872 c         4374 g         2696 t
ORIGIN      
        1 ggattcagat cgtcccccga gacgacctgc atgacctgta cctcctgcag cgcgtagacg
       61 atgtcctgaa gggtgctgtc gtgaagctcc ctggccagcc tgttgcgttc ggcttcccgg
      121 atctcctcga gcatgcgctc cgtccgcttg cgctctgtca catcgtgggc gacgacgcac
      181 atgacctttt cgccgttgta gggtacggcg ctcacgttga cctcgacatc agccagcgag
      241 ccgtccttgc gacggtactg cctctcaccg agggaacggt gaccctcaac cataatacgt
      301 ccgatgttga ggtccacact ctcctgatcg tgggcgacta tgtcgtagag cgtcatctct
      361 ttcagctcat cgtgcgtgta gcccaacgaa cggaggaggg cgtcgttagc gtcgagtaca
      421 cgcctactct tcgcatccac gaggaagatg ttctcctccg cctgctcgac caccgtgcgg
      481 tagagtgcct cgctctgcct cagggcctcc tcctggtgct tgcgctccgt tacgtccatg
      541 aggataccct gccagaagag aggcctaccc tcagagtccc tgaccagcat ggcctcctcg
      601 cgcacccaca ccacccgccc gtcgcgtgag atcctgcggt acaccacgct gtaaggctcg
      661 cccgtctcgt ccgtgcggat gtcctccctg agcacccacc cgcggtcttc gggatggatg
      721 gtctgccagc ggaggtccgg gtttgcgagg tattcttcag gcgagtagcc gagcacactc
      781 tcgatctgcg ggctgacgta ggcgagggtg gcattctcgg tctcctgcat gtaggtgacg
      841 gctggaagct gctcgaccag cgtccggtag cgttcctcgc tcatgatcag ggcctcttca
      901 gcctgctttc gctccgtaag gtctatcacg gaggtcaccc ggatccgacg ccctgaatag
      961 ggcaggtgcc tgggccttat ctcaatgggg aaagtggtgc cgtctctctt gaggcccatc
     1021 gactcgtatg gcctggtgtc ctcgtcggag atacgctgcc tcaccatctc cctgtactct
     1081 ggcgacgtga gctcgatggc gttccttccg atcagctcct cgagcccgta cccgaacatc
     1141 tcggtaacgc tacggttggc gtcgatgatc ttgccgttct cgctcaggat tattccctcg
     1201 acgacctcct gggcgagaac cttgaagcgc tcttcgctct gcctgagctc ctcctcggtc
     1261 cgcttctgct cggtgatgtc gtactggacg ccgagccagt acaaagcgtt gccttcttcg
     1321 tccctgatga gcgtcgcctc gtcgtgtacc cacaccaccc gcccgccgcg ggcgatctga
     1381 cggtactcga tcctgtaagg ctcgccggtc tcgtcggtcc ttagctcttc ggcgagcacc
     1441 ctctctctgt catcgggatg cagcaccctg agccagtgct cttcgtcgag tatctccttc
     1501 tcgggaggat aaccgagcat agcctcgtac tggggactca tgtacgttat ggccttcggg
     1561 gtgtcggagt cgatgggttc ctgcacgtaa gtgacggccg gaatctgctc cacgaggtgc
     1621 ctgaagcggt cctcgctcag gctcaacgcc ttctggaccg tctcgcgctc ccgcctcacc
     1681 tccacctccc tgagctcgcg ctcgatggcg gggcacagcc tgctcatgtt ctccttggtc
     1741 aggtagtcgt tggcccccgc cttcatgatc tccaccgccg cgtcctcccc tatcttgccg
     1801 gagacgacga tgaaagggac gtcgtaacca agctcccgta gtagcctcag ggcgtggggc
     1861 gccctgaagc gtggcaaata gtagtcggag atcacgacct ggaaaggctc gtctctggct
     1921 tccgccgaac gtaatgcttt ctccatgtct tctggggtac aaaccctctg cgagaggggc
     1981 tcgtatccgc cgcgtctgag ctcccgcagg agaagcatcg cgtcgttctc ggagtcctcg
     2041 acgagcagca cgcgcagggt ccctgtcagc ggcacactct tctcgccggc ctcacgcaac
     2101 ttcggccttc ttcgcaggga ccgcgcggtc cccgacatcc cggcctccta gcgtgaagta
     2161 gaacgtggcc ccctcaccca cggttccctc cgcccagacc ctgccgccgt gcctgtgaac
     2221 gattcgcgct accgtggcca ggcctatccc cgtcccctcg aactcatctt gcccgtgcag
     2281 cctctggaac gcgccgaaca gtttgtctgc gtacgtctgg tcgaagccgg cgccgttatc
     2341 ccgcacgtag taaacgggtg actgtagacc ctgaccgggt ttcctgtcgg cgccgaactc
     2401 tatgcgcgcc gcttctttcc ttgcggtgaa cttccacgcg ttgccgagca ggttctcgag
     2461 cgccaccttg agcaggctcg cgtcccccca ggccataatg ttctcaccgg ccgagaactc
     2521 gacctctcgt cctggctgcg acgccctaag ttcctcgagt atccccgcgg cgaggcttga
     2581 caggtccacg tgttccctgc gcaggggcct gcgtccaacc ctcgagaggt cgagcaggtc
     2641 gtctattagg gtggccatgt gcccactcgc cgcccttact cgaccgaggt agtccattcc
     2701 ctcgtcgtcg agcgcttgct cgtagtcctc ctgcaaaatc tgggagaagc cgtcaatggc
     2761 cctcaagggg gccctcaggt cgtgggagac agagtaagag aacgcctcga gctccctgtt
     2821 ggtcccttcc agttccctgt tggcgtcctc gaggtccttg ctcgcgcgct cggccaggat
     2881 gcggctgcgc accagcatcc aggagatgcc gaagagcaag acgctggctc ctatgccact
     2941 ggcaagcacg aaggccggca ggttgctctc ggcgccattc ttgaatcccg gcaacgtggc
     3001 gaagtacagg ctccactcgc gcccggccac cccgaagcgg ctcttctcgg agaagagagg
     3061 atcgtacccc tcttctcccg cgcgctccac cccgtcttcg tcgtagagga gcgagctcga
     3121 ctcgacgtcg gcaccgtcat atacctcgaa gtctatggca gggtcgaagc cactcccgaa
     3181 cacgtcgtcg agcagcccat cacgcctgaa atatcctacg acgaaccctt tgagcgcgcg
     3241 ccttcgtgcg gcgacattct ccaccggctc tccctcggcg tagaccggaa ggtacacggc
     3301 gaagccaggg ctgagggcga ggtcggcgct cgagttcgcg gttggtcccg tgagcacgta
     3361 gaccatcctg gtagcctgcg gagagccggt gtcgcgcgcc gcgtccatgg caaccttgtg
     3421 ggcctgctcc gtgtaggcat cctggtttat catgctcagg ttggcctcgt ccgtgggggc
     3481 aaccagcgcg aggggaaagt aggcggaacg ctccccgccg ggatccaggt caggcctcat
     3541 cgcaggcatc ccctctctcc tggcctcact gacgaatgcc gccctctcgg cgggccttac
     3601 atatctggcg aagccaagcg cctgcaggcc ttcgagccgg ctcttggtct ctatgccctc
     3661 gacgtagctc tcccactctt cgcgctcgac cgccttgctg gccaggaaca cgccgcgggc
     3721 gccgaacagc gcgtcgaggt aagcatccgt ccgccggtcc acggccgcct gcgtagcctg
     3781 aatggtctcg tcgaaacgta cctggtcctg cgcctcgacc gtctggcgca cgtagtacca
     3841 ggcgagccca gcgagcagga gcgagatcag gagcaccccg taagccgcca cagcgaggcg
     3901 caggtggcgc aagagccggc ccagcgctag gcgcacagag ggcaggtgcg ctctgaaccc
     3961 acctatgaga tcctccaacc tacgagcaac gtgcactgca ccgccacgaa cgcatccgtc
     4021 accgataacg ttgcgctcac cgctccacga acctttccat tcgacgaatg atgcccgggg
     4081 acaatccgca cgggacgcgc cgcatagagc catgttaccc ggcgtacaga tggcccgcaa
     4141 gatttgttcg ttctcgaggg tcgcgagcga atctctcgcc ccgggcgacg ttctgagaag
     4201 cgtcttgggt ctatcgccct atctcaccaa tctcatcctc gagcatcgcc cgcaggtgca
     4261 ggctcaccca cgtcctgtgc ggccgccagc tcctgcctaa tcgttcgagt tcttcggcag
     4321 acggaggagc atcaagaccg taagccatgc gcaaacgccc cgacctacgc ccctcgtacc
     4381 ttacgctcgc cccaaaagag taaggcccca caggacgcaa ctcgaaatac ccctcagcca
     4441 tctgtgctcc tctcgagtac tccgcgccgc cagcgcccga ccccgagcat tactcggaat
     4501 gcgtcggcca gatcttcgcg ggcggcaggg agttccgcgc gcagccattc gtcgaggtca
     4561 gcctccgccc gttcgtacgc cctttcgaag ccaggatcgt cgtagcgcct ggggaggaga
     4621 tccagcatcc tctgacgggc gcggaggctc gcgatgagat caccgagagc ggcggaggaa
     4681 ccgcggtcca accacatctc ctgctgctcg acgtacttct cgaggccgtc cacggagcgc
     4741 tctatatcct cgatcggcaa ctctcccgag gggccggggg cacggacgta ctccagagca
     4801 gcgtcgaact cctcgacgag cgggatcacc atcccctgga cgctctcggc gacctccacg
     4861 cggtgcctgt aaccgacctc gtactcggtc cgcttccgcg cgaaatagta tccgacgacc
     4921 gcgctcgcgc atgcgagcac cagatacgaa agcaacccgt aagtcattgg cggaagctgg
     4981 ctcacgctcc acccttcctc cccggaggat gcagcacgta gaccacgcgt ccttgcaccc
     5041 gcgcctcctc cgccggcacc accatctcct cgtactctcc cggcggagcc tctggcctca
     5101 gcctcaacgt ctccccctcc ctgtagaagc gttttaccgt cacctcctcg ccttctctca
     5161 acaggaccac gaccaccgag ccatccggtg gaatctcgtc ctcctccacc accaggagat
     5221 ccccatcctc tatcctcgcc cccaccatgc tctggcccac aacccgcaac agatacctcc
     5281 gcttgccaga acccgaagcg agtagctccg ccgccagcga gtaagcctca tcggtggcca
     5341 cggcttccag tccgcgaccg gcggcgatcc tcccgagcaa ccctatccgt ccagcaaccc
     5401 cccacccctt ctccgtgagc cggaccatcc tggcccctct gtcccgcacc acataaccct
     5461 cttcttctag cttcctcaag tgatggtgga ccgtctgcga gctcctcaac cccacctcga
     5521 ttccaacctc gcgcacgctc ggcggtggct ctccagcacc cgcccgccgc gccagatacc
     5581 gcaagatcct ctcgcgcctc ggatgtagag actcttccat ccaccgacac gttagcgcaa
     5641 cattcaatct atgtcaaaca tttgtttgat ccttaacgag atctgtggac gtttgactct
     5701 gccgatcatg cgaacggaag tttgttcgca gcaaggcatc ccatagaatc agcggagagt
     5761 tgcgggtcgc atggaggtct cggatgagtg tccctctcta tgctgcgtcc ggcatccccg
     5821 aagtgtggct catagacctg aactcggaca ccatcgaagt ctactcggag cccggtcccg
     5881 gagcctacga gaaatccgct cgcttcggac gtggcgatca ggtactctcc gccaccctgc
     5941 ctggtctcgc tatcgacgcc aacgtagccc tgccgccgaa aaagtgcatg tcagagatca
     6001 atccgcctct gacctcttgc cgaggtcgac gcctgggcga agctctttga ggtgtatgac
     6061 gtagctctcc ctgacgcgca tcccggccct tccgtagagc ctgggagcgc ctgtgacgct
     6121 ctcggcatca acgctgaggc tgaccctggg ggtgccgcgc cgatgatact cggtgaaggc
     6181 gtgacgaagg agggctagcc ccaacccgcg gttccgccac ggacgcctga ctcccacgac
     6241 gtccacccat ccctcgccgg cgagcgtctt acacagggtg acgcctgcga tctcgtcgcc
     6301 ttccagggcc aggaaccaga gggatggatc gaagctctct gctttcgtca tcccgacgaa
     6361 ccgttcgaac gtgccgcgcg gccttcccca catgtccctg aaggcatcct cgacggcctc
     6421 atagaccgca cgctcgtccc agcctgggac gaaggtgcgg acggagatat cggcgggcca
     6481 gtgtggtagt ggcggggcct cttcgagttt ggtctccatc acgtatatcc cgcgcaccgg
     6541 ggtgtagcct gagttctcca gcaggcgtcg cgctgcttcg ttggctgagt tgatgtaatg
     6601 ctgcgcgacg acgcgcgcgt tctcctgagc ttgtggcatg tggtcgcgag tccagcgttc
     6661 tccccacgcc accagatagc tgcccagacc catcccccga tagtccgggt ggacgtaacc
     6721 gtagatggag acgatcacga aggagcggtt gaacacgtcg gcgtaggcgg ctgtgcggcc
     6781 gtcgggggag gtcacgatca cggcctcctc agcgagatca agagagtgcc agtcgtcgtg
     6841 catctcctcc acgctcatgt ccgtctcgcc cgtatccgcg atctggcaag ccgagatgag
     6901 gattgccacc tcctccgcgt cttcgcgcgt gggtgctctc gtcgagaaac cttcaggcaa
     6961 tccgcgcata gcgggaagca taacactgcg gcctggagct acgaaaagta gagaccatga
     7021 acgaggacgc ggccccgatc acgatggact tcgcctccga caagccgctc atcccagtcg
     7081 ccaacctcgt aacagaaggc agcggggtca gtcgtaccct caacgccatg cccaagcaga
     7141 gcggcacggc gaccctcacg ctctccgacg gcgaggatac gggtacgctc ctcgtcaagg
     7201 ttcagctggc tatttatggt ttcccgccgg gagacgagat ccggcctttc agaacgtgat
     7261 cccaaacccg cgtagcgagc ccaggttggt cggacacacg gacaaaagag aggctcccca
     7321 caccgggagc ctcactcgct gctattgtgg agtccttaag atgagactct ctctttgacc
     7381 tggaaggtaa gctcctcgcg cccttcccct ttgtggtcca cgacgacggt gtcgccgggg
     7441 tcgaactcgc cgcctatgat gcggctagag agggggttct cgacgtagcg ccttatggcc
     7501 cgcgccagcg gtcgtgcgcc gaacttcggg tcgtagccct cctcggcgag gaactccttc
     7561 gcggcgtcgg tgacctcgat ctggacgcgc tggctttcca ggtgcttgtt caagtgagag
     7621 agcatgatgt ccacgatctt cagcacgtcc tccttggtga ggggctggaa gacgatgatc
     7681 tcatctaccc tgttgaggaa ctcaggcctg aaagctcttt ccagcgcttc cctggcccgc
     7741 cgctcggtct cgcggaagtc cacgccgtcg cccgaggtga agccgaactg cctcgtcgag
     7801 acgaggtgct ggctgcccac gttcgaggtc attatgacga cagtgttctc gaagttgacg
     7861 gtcctgccct tggcgtccgt gaggcgcccg tcgtcgagga cctgcaggag agtgttgaag
     7921 acgtcagggt gggccttctc gatctcgtcg aagaggacta ccgagtaggg tcgtctgcgg
     7981 acctgctccg tgagctgtcc cgcctcctcg tagccgacgt agccaggagg cgcgccgacc
     8041 aggcgcgaca ccgtgtgtga ctcctggaac tcggacatgt ctatgcggat catggcgtcc
     8101 tcctcgccga agaggtactc ggcgagcgtc ctcgcgagct cggtcttgcc cacgcccgtg
     8161 ggaccgagga agatgaagct gccgacgggg cgcttcggat ccttgatgcc cgccctggcg
     8221 cgcctgatgg cctcggccac agccttgacc gcgggctcct gccccacgac ccgctcgtgc
     8281 agtacagcct ccaggctcag gagacgctcg gcctcctcct tgacgatgtt ggtcgcagga
     8341 acgccggtcc actcctcgag gatgcgggcg atgtcctcgc gcgtcacctc gggggcattg
     8401 gtctcgcggc gtccggccca gccctcgtgc tgctcctcga gctcagcctt gagctgttcg
     8461 atacgctgct tgaagccagc ggctttctcg tagtcctcct ccctgacggc gtcctccttc
     8521 tcgcgctcga ggcctgctat ctcctcctcg atgcgcttga cgtcaacggg tggcaccgtc
     8581 gagcggagcc tcacctcggc agcggcctcg tcgaggagat ctatagcctt gtcgggcagg
     8641 aagcgatccg ttatgtagcg gtccccgagc tgggccgccg cgattatggc ctcctccgtt
     8701 atcttcaccc tgtggtgcgc ctcgtaacgg tccctgaggc cgaagaggat agccaccgtc
     8761 tcgtccaccg taggctcgtc gacgagcacc ggctggaagc gccgctctag ggctgcatcc
     8821 ttctctatgt tcttgcggta ctcgtcgagg gtcgtcgcac cgacgacctg gagctcgcca
     8881 cgcgccagcg caggcttcag catgttcgat gcgttgacgg cgccctccgc gccgcccgct
     8941 ccgacgatgg tgtggagctc gtcgataaac aggatgatat tcttctcctc ctgctggagc
     9001 tcctttagca tctgctgcat ccgctcctcg aagtccccgc ggaaacgggt gccggcgacg
     9061 agcccaccca cgtcgagcgc gaggacgcgc ttgcccctca gcgtctcggg tatgtcgtcc
     9121 gagacgatcc gctgggcgag cccctcggcc accgccgtct tgccgacgcc ggcctcaccg
     9181 atcaggacgg ggttgttctt cgtgcggcgg gagaggatgc ggacgacccg tgagatctcc
     9241 tcggctctcc cgataacggg gtcgagctcg ccgtcacttg ctagttgcgt gaggtctctg
     9301 ctcacctgat cgagggtcgg cgtccggctc ggcggaccct gctgaccgcc acccatccca
     9361 gggcctcctg cggcggcgcc aggcatcccc tgtcccatct gagccccccg ctgctgcatg
     9421 aggcgtagga tctcacgcct caacgcctcg gcgtcgttcg ccccgtgctg ctgcagtatc
     9481 ctgtaggcgt ttccgtcacc ctcaccgagc agcccgagca acaggtgctc actcccgacg
     9541 tgcccagcgc ccatctgcct ggccgcggtg aatgagagct gcaaagcgcg cttggcgctc
     9601 ggggtgaacg tgtagatctc ctctcccgga tcgccgcgga actcctggaa gcgcgcccgc
     9661 atcccgccga cgtcagcgcc agcgcgctgc agagcctcgg tcgccacgtt gtcaccggtc
     9721 acgatgccgg cgagcagatg gtccgtcccg attgccgccg tccgagtggt cccggcagac
     9781 gcctcgcggg cccgcatcac ggcctgctgc gcctcccccg agagcgcgtc gaacaagcta
     9841 ccctgccccc cttgctgctg ctgtattagg ttctggagca tcgcgaacgg gtccgacccg
     9901 ccctgcgcac ccccgatcat gccgaactgc cgcgcgcact gcgtgcacag atagctggtc
     9961 accctctggt caccctgctg ccgtaaaacc tgcacgctcg ccggccgaat accacagttc
    10021 tcacagatca tccgcccacc gcctcctgta ataaaatcct agaactctcg ttcccccctc
    10081 aggaggaacc gtaaggttga aatctaagcc tgtcacgctc atattttatt accccttagc
    10141 gtaacatcaa ggtagagtgg gctggcgtgt tctgcactgg gttcctgcat aatgggatgt
    10201 gaagaaagcg agaggaggct ggttgtgagg atagggattt tgaccagcgg gggcgacgcg
    10261 cctgggatga acgcggcggt aagggcggtg gcgaggacgg ccttgttccg gggttgggag
    10321 gtcatgggag tatgggccgg atacaggggg cttttggagg gtaggatcta cccgatggac
    10381 cggcgccgtc tcggggggat catccaccgt gggggcaccg tgctcggcac ggaacgctcg
    10441 gcggagttct cgacgcccga ggggcagagg agcgcggcgg gcaaactcgc cgaagccgag
    10501 gtcgaggggc tagttgtcat aggcgggaac gggagcctga ccgggggttt gaggctggcc
    10561 gagctggggg tcaaagtagc cggaattccg gcgaccatag acaacgacgt gtggggcacc
    10621 gacacggcca taggggtgga cacggcgctc aacacggcgc tcgaggcaat cgacaggatc
    10681 aaagacacgg ccagctccca ccagcgggcg cacgcggtcg aggtgatggg ccgcgactca
    10741 ggctatctgg ctctcatgag ctctatcgcc ggcggggccg aggccgccgt cgttcccgag
    10801 ttcgaaccaa ggcccgaagc actgttgtcg ttcctcaaga aagcctacga gcaggggaaa
    10861 tcccacttta tagtggtggt cgccgaaggg gcgccgctct ccgccgagga attgcacgag
    10921 tacataaacg agtccgaggg cacctacgac tcgaggctca cggtcgtagg tcacatccag
    10981 cgcggtggga tccccacggc ctcggaccgg atactcgcaa gccggctcgg gacggcagcg
    11041 gtcgaggcgc tcgccgacgg agagaccggg gtaatggtcg ggatcagggg cccgcgcatc
    11101 gagcgcgggc cgctcgaaga ggtggtaagc aaagagcaac ccctggactc cgatctctac
    11161 aggatggccg aggtcctggc cgagctgcct gagtaagccc tcgggtacgg ggtatatctt
    11221 tccaaacgga aggaagctac ttcggaggac gacttgcagg aacgaagtct catcgggctc
    11281 gaggcgcggg cggcggacgg tagcgaggtc ggccggatcg ccgatttggt agaagacgag
    11341 gagagcggcg aggtcaccca cgtcgtcgtc gagacggaga ggggggagtg gctggaggtt
    11401 cccatcacgg acctctccct cgaccccgac gccgacttcg cgaccttcga ggccgacgct
    11461 tctgacgaag agcccggcga ccacacgggt gacgaagcgg aaccagaaga ctactacgcc
    11521 cccgacgaca tcgacgacga gaacgaagaa gatttcgagc acgaaggcca gctcgtcacc
    11581 gagccagagg acggtcacga tcccagctcg gaggaggatc tggaggcgcg cgaggattgg
    11641 gaggaccaca gctacacccc agactcgggg tacccgcgca acgacgtcta catcgaccct
    11701 gatacaggcg acgaggagat agacccactc ctcgaagaga acgatgacgt caaggacgac
    11761 gtggaggacc tgctcgacag cacgagcctg gaggtccgct ccgtcaggga aggcgtcgtg
    11821 gagctgacag gtacggcagc cacccaggat gacctcgaag agacggtgcg tgagatcatg
    11881 ggcctcgacg aagtcctcga ggtcgatacc acggacgtag aggtgagata gcctttattc
    11941 cacgtttgtt gcttcagggg agttcatgcg gaggatatcc cccatgagga gtcgatcgag
    12001 ccgggctgaa gtacaaaggc gctccgcctt ggccagtctt tcggcccatc aacctcgcct
    12061 cgagcctgcc gatacctgct tcccgatgcc cgacgtcacg atccacccct gctgcgcgaa
    12121 gtacctggcg taagtctgca tcagcgttcc atcgatggcc ggccgtgttg gtccaccgtc
    12181 gctcagggcg cggcgcgtgt tatggtcgtc gtaggtgttg tcgtcacgta gatcttctgc
    12241 cgcaggcgcg gcggcgcgca cgacctcgcc gggtgaacct tcctcagggg gagctgtgtc
    12301 taacctctgc agccattggt cgtggggaac acgctccagc ctgtggccct gttcctccaa
    12361 ccagtcgaaa acctcccccg cgggaggagg ttctggattc gccacgtggt acgtttctcc
    12421 ctctgctgca gggtccgatg cgatgtgaag tatcgctgcg gccacgaagt cgacgggcgt
    12481 catctccgta ttccaaccat ctatctcggg ggcgcactcc aagcgcagag actccacaac
    12541 aacggcgcca aggaggtcgc gcgggttgga ggctccggtc tcgctgtgcc ctgaaacgtt
    12601 accgggacgg tagacgcaaa caggaacacc gcgtccggcg gcctcccgca ccagtttctc
    12661 tgcgacccac ttggaccggc cgtagccgtc ctcgcgggcg ccggcgaggg cgtcgagatc
    12721 ggcgccttcc tcgcagcgcc ccatgcccgg cgggaagatg ccgttcgtgg agacgtggtg
    12781 cacgagcttc gccccgtggc ggcacgccag gcgcaggact tctcgggtgc cgccaacgtt
    12841 ggccggcttc agctccgaat aagggtagac gaggttcact accgcgccgg cgtggaagat
    12901 cagacctacc tcgcgggcca gggcgtcgaa gtccccctcg tccaggccga acaggggttt
    12961 tccgaggtct ccccggacgg ctaggatacg ctccgcccgt tcgggtttcc acagcccgta
    13021 gcgccgcagg ttggcctcga tagactccat cccgccggcg tccccgcgag gcctgacgag
    13081 gcagtggacg cgggcgttag tgcccgagag cagcccgtcg agcaggaagg caccgagaaa
    13141 gcctgttacc cccgtaagga acacgtcgct agtgtcacgc agggtcggcg taccgttagc
    13201 ggcgggctga gggactatcc ccgggtcgag gacggcctcg gcggtgagat cctccgcgtc
    13261 cgtcacccct ccagatgtcc cgtcgcgttg tagctcctct atcctctcga gcagcccgcc
    13321 gagcgtcggg tcttccatga atagtggcat cgagacgtgg acgccgaagc cctgctcgac
    13381 gaggccggag agctgcgcgg cggcgagcga gtgcccgccg aggtcgaaga agtcgtcctc
    13441 gggccgcacc tcgccctggt ccaggcgcag cacgtcctcc caggaccgcg tgagcagcgc
    13501 ctccttctcc ctgcgggggg cgctttcggg gagcctgtcg acgctgcccg tgtcttgcct
    13561 gggggagcgg gcggggcggg gcggcggggg cggtagctcc gcacggtcga ccttgcccga
    13621 ggtggtctgg atggggagcg actcaagctc gacgaagacg gaaggtaccg cgtagtgcgg
    13681 caggctgtcc tgcagtacgg accttatctc cttgctccgt cccgtcctgc ggtcgaggct
    13741 ccagccggag tagcgcccct cgtcgtcggg gtccggcacg atgtaggcga cgaggcgttt
    13801 gtcctccccc tcctcgccct cggagaccac gacgcaggac cttacggcga gactcttctc
    13861 gatggcggcc tcgacggctc cgagctcgac gctgtagccc cggatcttga ccatgaagtc
    13921 cacgcggccg aggatctcca ggctcccatc ggagaggctg cgggccctgt cgcccgtgcg
    13981 gtacatcctt gcaccgctcc tggaggagaa ggggtcttct gggaaactct cctcggtctt
    14041 ctcgggcagg tcgacgtagc cgcgggcgag acagtcgccg ccgacgaata gctccccagc
    14101 ctcgccctcg ggaacgcgtt cgccgtcaga gtcgagtaga tagaggtgct cctggtccat
    14161 gggaacaccg atggcgcagt acgtagcctc tgggttctcg accagctcgc gcaggtcccc
    14221 ggcggccacc tcgtgggtct cgctgcagct gtagacgttg agcaggcgcg catctggcaa
    14281 caggtccatt gcgcgccgcg cgagcgtgcg agttacgacc tcgccgttca gccaaagcgt
    14341 cctgagcttc gcgagcctct cgcccacgtc gggcccgctc gagttgagga cggcttcgag
    14401 gagtgatgg
//
LOCUS       DASTKM010000322         4813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1370990, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000322 DASTKM010000000
VERSION     DASTKM010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370990"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFQ10_12755"
     CDS             <1..810
                     /locus_tag="VFQ10_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966257.1"
                     /translation="PSLFLEFARLAEQRDPPEASKDWISRRGLLGLHKRERPKSDERA
                     LEAGETSFRHERWWFENDEYTEYMHEGGSSERAFDLQREVIGANHVLTQWEAFLSGEP
                     ERAEHELLDHFPQHHSELKQRYISFFRSRARFLGLTYKESLLYHVGFVACLRVQEVLE
                     AFAYPYLAFDYGNPYAPLHGTPGPGLAAGSWRPRNLLGAMYLQMYQVMISSDKLSQCR
                     YCGRIISYAPPIPGSATSRKPRKDKEFCDSRCRQNYHYHNRIKPRRQSEGG"
     gene            complement(1748..2710)
                     /locus_tag="VFQ10_12760"
     CDS             complement(1748..2710)
                     /locus_tag="VFQ10_12760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HET9966258.1"
                     /translation="MGGNDKLRVVAFGGGTGLPVLLRGLRDRVGDLAAVVTVADDGGS
                     SGRLRQELGVAPPGDVRNCLVALAGRKNLAEVFNYRFEGGGDLSDHSVGNIIIAALAD
                     MAGGFCEGVEQAARFLRIKGRVYPAAMESLTLVVRYADGTLTRGESVVHEIGKAVSEV
                     SVEPAGAAAPAGVISAIESADVVVLSPGSLFTSTIPALLGAGVREALAAFSGPVVYVA
                     NVMTQPGETAGFSVSDHLRALADHAGPVVTDVLVHAGGMPEELVARYAAEGAALVEVD
                     REEIAAVGVRLHEAGLLPYEIGASVRHDPDRLAREVCEAVLVRF"
     gene            complement(2932..3939)
                     /gene="gap"
                     /locus_tag="VFQ10_12765"
     CDS             complement(2932..3939)
                     /gene="gap"
                     /locus_tag="VFQ10_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET9966259.1"
                     /translation="MAVRVGINGFGRIGMLTAKAAIERSDDDIQIVAINDLMPMESLA
                     LLFKRDSVHGIWHEDVQLDGNILRVGDREIKTFAERDPAQIPWGDVGADLVVESTGVF
                     TDGEKAAAHIEGGAKKVIISAPAKNVDATFVFKVNHEDYDPESHNVVSNASCTTNCII
                     PMVKVLQENFGIESGYMTTVHAYTNDQSLLDAAHKDPRRARSAPNNIVPTSTGAARTA
                     GVIYPDLQGKVDGMAMRVPVPDGSITDFVARLNTEASVDDVNGAFKQAGDGELSGILE
                     YSDAPVVSSDIVGNPASCIFDPALTMANGNNVKVLGWYDNEWGYSNRTVDLAQYMGEK
                     L"
     gene            complement(4023..4250)
                     /locus_tag="VFQ10_12770"
     CDS             complement(4023..4250)
                     /locus_tag="VFQ10_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966260.1"
                     /translation="MDSGTIPSGWSRRCARLSSFASEVRRQLATGTASGRRRTDRAEL
                     AELVDAAGDWDARGVTFRTSSGAVARLVVRL"
     gene            complement(4315..>4813)
                     /locus_tag="VFQ10_12775"
     CDS             complement(4315..>4813)
                     /locus_tag="VFQ10_12775"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET9966261.1"
                     /translation="LAVAIGTSHGAYKFTKEPDREVLAMHIIEEINQRLPTTHLVMHG
                     SSSVPKELVDLINRYGGEIRPTWGVPVYEIQEGIKHGVRKINVDTDLRLAATGAIRKA
                     FAEDPSEFDPRYYLKPAREAMKGVVRDRMEAFGLAGHAGDYEPITLEEMAERYKEEGH
                     LLTTT"
BASE COUNT          865 a         1570 c         1459 g          919 t
ORIGIN      
        1 cctagcctgt ttctcgagtt tgcacggctt gcggagcaaa gagatccgcc ggaagcctca
       61 aaagattgga tctcccggcg cgggctacta ggactgcaca agcgagagcg ccccaagtcc
      121 gacgagaggg cactggaagc cggagaaacg agctttcgcc acgaacgctg gtggtttgaa
      181 aacgacgagt acaccgagta catgcacgaa ggcggatctt ctgaacgtgc ttttgatctc
      241 caacgtgagg taatcggtgc caatcatgta ttgacccaat gggaagcgtt tctctccggg
      301 gaacccgaga gagcagaaca tgagttgctc gaccactttc ctcaacacca ttctgagcta
      361 aagcagcgct acatatcgtt cttcagaagc cgagctaggt ttcttggact aacctacaag
      421 gaaagcctcc tgtatcacgt gggattcgtt gcctgtctga gggtacagga ggtcttggag
      481 gcttttgcgt acccttacct tgccttcgac tacggtaacc cgtacgcacc tctacacggc
      541 acacctggac cagggctagc agcaggctca tggcgtcccc gtaacctctt aggggccatg
      601 tatctacaga tgtatcaggt gatgatctct tctgacaagc tctctcaatg caggtattgc
      661 ggacgtatta tctcctacgc gcccccaatc cccgggagcg ctacatctcg caagcctcgc
      721 aaggacaaag agttctgcga cagccgatgc cgccagaact accactacca caaccgcatc
      781 aagccccgcc gccaaagcga gggcggttga cagagatttg acagttaccg gggccaacgt
      841 ctgcgaacat cgaaaaacgt tctatttgaa ggtttggctt gaataagcga atttctgaac
      901 cattgcgaac aactggaaac acgctgcttc tgactacgaa tcaggaggtt gcaggttcga
      961 gtcctgccaa gcgcgccaca aaaagcctcg ttttgcggga cttttggctc tatccgatgg
     1021 gccgaccgtc tgacgtacac cactcgttta ccacttatgg tttccgcggc gactccgcag
     1081 gacactgatc ggtgcagccg atcccttggt ccatggtcac ctagagcgat ctacgagtat
     1141 gggtctaaag accgttatga gcgtccggga cggttcttgg gccaatagcc gaagcggccg
     1201 caatcggggt tattaggcgc cttttctccg tctcttggca ggcagcaatc gcctggcgag
     1261 gcaaggcttg aggctcgact gtattaccga aagcaacggg gcgccctccc gtgagcctcg
     1321 cacccgccgg attgatcttg agatgtgatt cattgcctct ttcgggaaca gatcatggtg
     1381 tggtccggac aaggtaccta ttggccgcag ggttcgtatt cggggccttg ccagagtacc
     1441 agcgccgtgc gcactgggcc tgaatacagg ctgcgaagag cccacgcatg ggtcgaggcg
     1501 gcaaaggaag gatcgtcaga tgtgtaaggc cccgactggg caagtcgggg ccttacaaag
     1561 ggtcgcatac cgtgccagat aattgtgaag gctctcttct tcaagccaaa gtggtgcatg
     1621 caagccgatg acgcccgatt cgtcgtgcca gaaagtgacg cttatctggc caactggctg
     1681 cgataccgtg gtggtgatct cggatcgagg cgctcaccac tcgaagcctt gatccctggc
     1741 ccagggttca gaagcgaacg aggacagcct cacacacctc cctcgcaagc cggtcggggt
     1801 cgtgacggac gctcgcaccg atctcgtacg gaagaagccc ggcctcgtgc agccggacgc
     1861 ccacggccgc gatctcctcg cggtccacct ccaccagcgc ggccccctcc gccgcgtagc
     1921 gggcgaccag ctcctcgggc atcccgccgg cgtgcacgag cacgtcggtc acgaccggcc
     1981 cggcgtggtc ggcgagtgcc cgcaggtgat ccgagacgga gaaaccggcg gtctcccccg
     2041 gctgggtcat cacgttcgcc acgtacacga ccggcccgga gaaggcggcg agcgcctcgc
     2101 ggacccccgc gccgagcagg gcggggatcg tgctcgtaaa gagactcccc gggctcagga
     2161 ccaccacgtc ggccgactct atggcggaga tcacaccggc gggcgccgcg gccccggcgg
     2221 gctccaccga gacctccgag actgccttgc cgatctcgtg caccaccgac tccccccggg
     2281 ttagcgtgcc gtcggcgtag cggaccacga gcgtgaggct ctccatcgcc gccgggtaga
     2341 cgcgcccttt gatcctcaga aaacgcgccg cctgctccac gccctcgcag aaccccccgg
     2401 ccatgtcggc gagcgccgcg atgatgatgt tgccgacgct gtggtcggag aggtcgcccc
     2461 cgccctcgaa gcggtagttg aagacctcgg ccaggttctt gcgcccggcg agggcgacca
     2521 ggcagttgcg cacgtcgccc ggcggcgcca ccccgagctc ctgcctcagt cggccgctcg
     2581 agccgccgtc gtccgccacg gtgacgaccg cggcgaggtc gccgacgcga tcgcgcaacc
     2641 ctcgtagcag caccggaagc cccgtaccgc cgccgaacgc caccacccgc aacttgtcgt
     2701 tcccacccat ggactttcag tatatcctcg tccggcccag gctggcagtc cgaagtcgcc
     2761 cgcttggact cgccgacgga tcactgagca gatgcttggt cctgctcgag attaggaacg
     2821 gcttgcgcca ctcaggcttc gtagcatctc acctccctgc ttctaatcct caagccgcgg
     2881 tccggggacg caacccgccc tcggacctgc aaaacttgtt caggttgcgg actacaactt
     2941 ctcacccatg tactgcgcca ggtccacggt gcggttcgag tagccccact cgttgtcgta
     3001 ccagccgagc accttgacgt tgttaccgtt ggccatggtc agcgcagggt cgaagatgca
     3061 gctcgccggg ttgccgacga tgtcgctgga gacgaccggt gcgtcggagt actccaggat
     3121 gcccgagagc tcgccgtcgc cggcctgctt gaatgcgccg ttgacgtcgt ccacgctcgc
     3181 ctcggtgttg aggcgggcga cgaagtccgt aatggacccg tcgggcaccg ggacacgcat
     3241 cgccatgccg tccaccttgc cctggaggtc cgggtagatc acacccgccg tccgcgcggc
     3301 gcccgtggag gtcgggacga tgttgttcgg agcggaccgg gcgcgcctgg ggtccttgtg
     3361 cgccgcatcg aggaggctct ggtcgttggt gtaggcgtgc acggtagtca tatacccgga
     3421 ctctatgccg aagttctcct gcaggacctt caccatcggg atgatgcagt tggtggtgca
     3481 gctcgcgttg gagacgacgt tgtgcgattc ggggtcgtag tcttcgtggt tgaccttgaa
     3541 gacgaaggtg gcgtcgacgt tcttggcggg ggcggagatg atcaccttct tcgcgccgcc
     3601 ctctatgtgc gcggcggcct tctcgccgtc ggtgaagacg cccgtggact ccacgaccag
     3661 gtccgcgccc acgtcgcccc agggaatctg ggccgggtcg cgctcggcga aggtctttat
     3721 ctcgcggtcg ccgaccctga ggatgttacc gtcgagctgt acgtcctcgt gccagatccc
     3781 gtgtacggag tcacgcttga agagaagcgc caggctctcc atcggcatca ggtcattgat
     3841 cgcaacgatc tggatgtcgt cgtcgctgcg ctctatcgcg gccttggcgg tcagcatccc
     3901 gatccgtccg aacccgttta tgcctactct tacggccact cttttctcct gtctgtcggt
     3961 tgcaaccatc atttttattg tgcccagacc tccgggcgac agcacaggcc gaccttcgag
     4021 cgctacaggc gcaccaccaa gcgcgcgacg gcgccgctcg aggtgcggaa ggtcacgccc
     4081 cgggcgtccc agtcaccggc ggcatccacc agctcggcca gctccgcgcg gtcggtccgc
     4141 cggcggccgg aggcggtgcc ggtggcgagc tggcgccgca cttcagaggc gaacgaggac
     4201 agcctcgcac acctccttga ccagccgctc gggatcgtgc cggagtccaa cttcgatctc
     4261 ctaccaggct gcacgtggcc agccgaggat cgacccgtca gaagccagca gccactaggt
     4321 ggtcgtgagc agatggccct cctccttgta gcgctcggcc atctcctcca gcgttatcgg
     4381 ctcgtagtca ccggcgtggc cggccagccc aaaagcctcc atacggtccc tgacgacccc
     4441 cttcatcgcc tcgcgggcgg gcttgaggta gtagcgcggg tcgaactccg aggggtcctc
     4501 ggcaaacgcc ttgcggatcg cgccagtggc ggccagcctc aggtcggtgt cgacgttgat
     4561 cttcctgacg ccgtgcttta tgccctcctg gatctcatag accggcacgc cccacgtcgg
     4621 ccttatctct cccccgtagc ggttgattaa gtccacgagc tccttgggca cgctggagga
     4681 gccgtgcatg accaggtgcg tggttggcag ccgctggttg atctcctcga tgatgtgcat
     4741 agccagcacc tcgcggtcgg gctccttggt gaacttgtag gccccgtggc tcgtccctat
     4801 ggcgaccgcc agg
//
LOCUS       DASTKM010000323         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_846989, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000323 DASTKM010000000
VERSION     DASTKM010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846989"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2276
                     /locus_tag="VFQ10_12780"
     CDS             <1..>2276
                     /locus_tag="VFQ10_12780"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HET9966262.1"
                     /translation="QSAFRILVAASERDLAAEEDLLWDSGRVESDRTIGVEYEGEALR
                     SGSQCVWKVRVWDGANNPSPYSRPAVFEMGLLEKSDWEGAWISAGKDPSGDLEPPSGE
                     EYDDLANGLAPSPNLRREFPLDKPVNRARLYATARGVYELYINGNRVGNDVLAPGWTD
                     YDRRIQYQTYDVTPLLADGPNALGAILGDGWFAGFVGFDPKHRGAHYGTRPQLLAQLN
                     VEYEDGTTESLATEGSWRCSTGPILYSDLLMGESYDARREMPGWAEPGFDDSGWYGVE
                     VEEIGDTNLVAQPDEGIRVTEELEAKTVTEPESGRYVLDLGRNMVGWVRLKVEGEAGT
                     EVILRHAEALNPDGTIYTTNLRSARATDRYVLGGDGEEIYEPRFTFHGFRYVEITGYP
                     GEPPPGAVTGRVVHSATPPSGSFECSSRMVNKLQENIVWGQRGNFLSVPTDCPQRDER
                     LGWMGDAQVFVRTASFNMDVAAFFEKWMTDVEDAQSPEGAFPDVAPLPGGSGLIDLRW
                     GAPAWGDAGVIVPWTIYRTYDDTRIVERHYDAMTRWMEYLHEANPDLIRKNRMGNNYG
                     DWLSPKGDHTPKHLLATAYWAYGAKLIAEMAEATGRNEDAREYRDLNERIKAAFQAAY
                     VFPDGRIEGDTQTCYLLALHMGLLPEDLRSTAAEHLVKSIEREDWHLSTGFAGVGFLC
                     PVLTEAGYTEVAYRLLGNETYPSWGYTIKNGATTIWERWDGWTEENGFQSPNMNSFNH
                     YSLGSVGEWLYRYVAGID"
BASE COUNT          465 a          712 c          724 g          375 t
ORIGIN      
        1 tgcagtcggc cttccgcatc ctggtcgccg ctagcgaaag ggacctcgca gcggaggagg
       61 acctgctgtg ggacagcggc agggtcgagt cagaccgcac catcggcgtc gagtacgagg
      121 gcgaggcgct gcgatcgggc tcgcaatgcg tgtggaaggt gcgtgtctgg gacggggcca
      181 acaacccttc gccctacagc cgcccggccg tcttcgagat gggcctgctt gagaagtcgg
      241 actgggaggg agcctggatc tccgcaggca aagacccgtc aggggatttg gaaccaccct
      301 cgggggaaga gtacgatgac ctggccaacg gactcgcccc gagccctaac ctgcgcaggg
      361 aatttccact ggacaaaccg gtcaacagag cccgcctcta cgcgacagcc cgcggcgtct
      421 atgaactgta cataaacggg aacagggtag gaaacgacgt gctcgcccca ggctggactg
      481 actacgacag gcgcatccag taccagacct acgacgtcac gccgctattg gctgatggcc
      541 ccaacgccct cggcgccatc ctcggcgacg gttggttcgc aggattcgtc ggtttcgacc
      601 ccaaacacag gggtgcccac tacggcactc gcccgcagct tctcgcgcag ctcaacgtcg
      661 agtacgagga cggcacgaca gagagcctcg cgacagaagg ctcgtggaga tgctccacag
      721 gcccgatcct ctactccgac ctcctcatgg gcgagtcata cgacgcccgt agggagatgc
      781 ctggttgggc agagcccgga ttcgacgact ctggatggta cggggtggaa gtagaggaga
      841 ttggggacac caacctcgtc gcccagccgg acgagggcat cagggtcacc gaagagctgg
      901 aagcgaagac cgtcaccgag ccagagagcg gccgttacgt cctcgacctg ggacggaaca
      961 tggtagggtg ggtgcgtctg aaggtcgaag gcgaggcagg aacggaggtg atcctgcgcc
     1021 atgccgaggc tctcaacccc gacggtacga tctacaccac gaacctgcgc tctgccaggg
     1081 cgacggaccg ttacgtgctt gggggcgatg gtgaggagat ctacgagccg cgtttcacgt
     1141 ttcacggctt ccgctacgtc gagatcacag gctatcctgg cgagccgccg cccggggcgg
     1201 tcacaggccg cgtagtacac tcggcaacgc ccccttcagg gtccttcgag tgctcgagcc
     1261 gtatggtcaa caaactacag gagaacatcg tctggggcca gcgcggcaac ttcctcagcg
     1321 tgccgaccga ctgcccgcag cgcgacgagc gcctcggctg gatgggggac gcccaggtct
     1381 tcgtccgcac cgcctccttc aacatggacg tcgccgcctt cttcgagaag tggatgaccg
     1441 atgtcgagga cgcccagtct ccggagggcg ccttccccga tgtggccccc ctgccaggag
     1501 gttcaggact catcgacctg aggtggggcg caccggcctg gggtgacgct ggcgttatcg
     1561 tgccgtggac gatctatcgt acttacgacg acacgcgcat cgtcgagcgc cactacgacg
     1621 ctatgacccg ctggatggag tatctgcacg aggccaatcc cgacctcatt cgcaagaaca
     1681 ggatgggaaa caactatggc gactggctct ccccgaaagg ggatcatacc cccaaacatc
     1741 tcctcgccac agcttactgg gcctacggcg cgaagctgat tgccgagatg gcagaggcca
     1801 cgggccgcaa tgaggacgcc agggaataca gggacctcaa cgagcggatc aaggccgcct
     1861 tccaagcggc ttacgtcttt cctgatggcc ggatagaagg ggatacccag acctgctacc
     1921 tcctcgccct tcacatgggc cttctaccag aagacctgag atccaccgcc gcggaacacc
     1981 tggtgaagtc catagagagg gaagactggc acctctcgac ggggttcgca ggcgtcggct
     2041 tcctgtgtcc tgtcctcact gaggccggat atacagaagt cgcctatcgt ctgctgggca
     2101 atgagaccta cccttcgtgg gggtacacca taaagaacgg ggccaccacc atctgggagc
     2161 gctgggacgg ctggacggag gagaacggct tccagtcgcc gaacatgaac tccttcaacc
     2221 actactctct gggctcggtg ggcgagtggc tgtaccgtta cgtggccggt atagac
//
LOCUS       DASTKM010000324         3545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_490942, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000324 DASTKM010000000
VERSION     DASTKM010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490942"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFQ10_12785"
     CDS             complement(<1..603)
                     /locus_tag="VFQ10_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9966263.1"
                     /translation="MTPPAEMSGQHETARQQSRAAWGAVARGWYAQREELWKASRPVS
                     EWMVRRLDPKPGDTVLELAAGLADTGLMAARLVGESGRVIVTDFTSEMVAAARRRAEE
                     LGVQNAEFRVLDAERMDLKTESVDGVLCRWAYMLMIDPSAAFAETRRVLRPGGRLAFS
                     VWAAREHNPALSLVGRVLESQGHIPPPDPEAPSAFAMADPG"
     gene            complement(1089..1481)
                     /locus_tag="VFQ10_12790"
     CDS             complement(1089..1481)
                     /locus_tag="VFQ10_12790"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9966264.1"
                     /translation="MSAQENKTLVRREQQELWNHTGDLDAADELFAADQAEAAKHQAA
                     HFRRGFPDVVSTIEDIIAEGDKVVARWRSRATHQGQYMGTPASGKEVELTGISIYRIE
                     EGKIAEEWSVEDEYGLMAQIGAVAETEQ"
     gene            complement(1612..2904)
                     /locus_tag="VFQ10_12795"
     CDS             complement(1612..2904)
                     /locus_tag="VFQ10_12795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9966265.1"
                     /translation="MQGLMNRKLYYGWVVVWVVFAALLVSAGVRAAPTVLINPLESEL
                     GWGRAAISAAISIGLLLYGLSGPAAGWLMDRFGPKPLTLFGLGLIGLSTLMGAAITQL
                     WQLNLFWGILSGLGTGVVAPVLGATVANRWFVERRGLVLGIFGAAASAGQLVTVPALM
                     WLVVQIGWRAGTVVLGAIVLLVLVPVLLLMRDDPSSVGLRPYGEPDESTAEESPAPEP
                     STAVAQQPGPGGSPLLGALRSPVFWLLSGSFFICGASSNGIIGVHFVPHSIDHGIPEV
                     TAASVLAIMGAMNFVGTLASGWLTDRYDPRKLLAIYYSLRGLSLLLLPFVTEFAGLTV
                     FAVFFGLDYIATVPPTVALVADRFGRANVGAIFGWVFFGHQIGAALASYLGGVVRISL
                     GDYTAAFLGAGVLAILASLMVLVIRREPTPFGPETARA"
BASE COUNT          686 a         1170 c         1075 g          614 t
ORIGIN      
        1 ccccggatcg gccatagcga aggcgctggg cgcctcgggg tccggcggcg gaatatgtcc
       61 ttgcgattcc agtaccctgc ccacgagcga gagagccggg ttgtgttcgc gggcagccca
      121 gacggagaaa gctagccgac ctccaggacg cagcacgcgc cgggtttccg caaaagccgc
      181 agagggatcg atcatcagca tgtaggccca gcgacagagg acgccgtcga cgctttccgt
      241 cttgaggtcc attcgctcgg catcgagtac cctgaactcc gcgttctgca cgcccagctc
      301 ctccgcccgc cgacgggccg cagcgaccat ctcggacgtg aaatccgtga ctatgacacg
      361 ccctgactca cccaccaagc gtgccgccat gagcccggtg tcggccagac cggcggccag
      421 ttcgagcacg gtgtcccccg gcttaggatc gagtcttcgg accatccact cgctgactgg
      481 acgagaggcc ttccagagtt cttcgcgctg agcgtaccag ccgcgagcca cagcgcccca
      541 tgcagcccta ctttgctggc gtgccgtttc atgctgcccc gacatctcgg caggcggtgt
      601 catgtcactc tcccttatgc gtctctctcg tgtaccaaga tagagagccc acaaattcgg
      661 cgcaaactgt ttgcattcaa accgggtttt gttacccctc caatccttac cacgcccgtc
      721 gagacgttcc gcctcacctc actgctcaga tacgcggcgg cgtcaacggg cgccgttgtc
      781 gcgaccggct ccgcctcggt caacgccgat tgcgacaacg gcgttgccct tcaccataca
      841 gctcagcctc gaaggggtca tgagcatgcg gctaagcgcg tagaggcgag cgtagttctc
      901 gcccagttgc tagcccaggg aaccagcgtg gaggacgaag tgcctgaggc catggccaac
      961 gctggcatat acctcgtcgt ggcgccagcc tatgaggcac gggagcttaa caccagcaca
     1021 gtgccatagg ctgggcgggg gaggcgctct ggctgtagcg gaatgcagta gtgtggctag
     1081 agatcctcct attgctcggt ctcagcaacc gcgccgatct gagccatcaa gccatactca
     1141 tcctccacac tccactcctc ggcgatcttg ccttcctcta tgcggtagat ggagatgccc
     1201 gtgagttcca cctccttgcc gctggcagga gtgcccatat actgcccctg gtgggtggct
     1261 cggctcctcc agcgagcaac caccttgtcc ccctcggcta ttatgtcctc aatggtgctt
     1321 accacatccg ggaagcctct gcggaaatgt gctgcctggt gcttggcagc ctcggcctga
     1381 tcggcagcga agagttcgtc tgctgcgtcg aggtcaccgg tatggttcca gagctcttgt
     1441 tgttccctac gcaccagggt cttgttctct tgtgcagaca ttttcgtctc ctcccgatcc
     1501 gctttgcaca acgaaccgca acacaccgtg catgatgcca tcaggcgaga gtcagcgcac
     1561 cccgacatga cgccagtatc tcggctgcgg attaccgaca ccacttcttc tctacgctct
     1621 ggcggtctcg ggtccaaaag gagtgggctc gcgcctgatg acgaggacca tgagcgaggc
     1681 gaggatggcc agtactccgg ctcccaggaa cgccgccgtg tagtcgccca gggagatgcg
     1741 gaccactcct cccaggtagg aggcgagcgc cgctccgatc tggtgtccga agaaaaccca
     1801 gccgaagatc gcccccacgt tcgctcgtcc gaagcggtcc gctaccagcg ccactgtcgg
     1861 aggcacggtc gctatgtagt ccagcccgaa gaagaccgcg aatacggtca accccgcgaa
     1921 ctccgtgaca aacggcagga gcagcaagga gagccccctg agcgagtagt agatggccag
     1981 gagcttcctc ggatcgtagc ggtcggtgag ccagcccgaa gccagcgtgc cgacgaagtt
     2041 catcgcgccc atgatggcca ggacgctcgc ggccgtgacc tcgggaatgc cgtgatctat
     2101 ggagtggggg acgaagtgga cccctattat gccgttggag gaggcgccac agatgaagaa
     2161 gctccccgat agcagccaga acaccgggct gcggagggcg cccaggagag ggctgccgcc
     2221 cggccccggc tgctgtgcca cggcggtgga aggctcgggg gcggggctct cttctgcggt
     2281 gctctcgtcc ggctcgccgt agggccgcag gcccaccgag gacgggtcat cgcgcatcaa
     2341 gaggagtacg gggacgagca cgagcaggac gatcgcgccg aggactaccg taccggctct
     2401 ccacccgatc tgcacgacga gccacatcag ggcgggcacc gtgaccaact gaccggcaga
     2461 ggccgcagcc ccgaagatgc cgaggacgag gccccgccgc tccacgaacc agcggttggc
     2521 taccgtcgct cccaggacgg gagcgacgac accggtgccc aggccgctaa gtattcccca
     2581 gaacagattg agctgccaca gctgggttat ggccgcgccc atcaaggtgc tcaagccgat
     2641 aaggcctaga ccgaagagcg taaggggctt ggggccaaag cggtccatca gccagccggc
     2701 ggcaggcccg gagaggccgt agagcaggag cccgatcgag atcgcggcgg agatcgcagc
     2761 ccggccccag ccgagctcgg actcgagtgg gtttatgagc accgtcggtg ccgctcgcac
     2821 tccagccgag acgagcagcg cggcaaacac cacccacacg accacccagc cgtagtagag
     2881 cttccggttc atcaaccctt gcaacaagct cctttccgcc atttgggctt acgagggagc
     2941 ccacttcgtt tcgcgcccgc gtgtgacgag gagcatacca attgagtgct gtgcggcgca
     3001 tcctccaaag gagtgatcct tgcggcgtgt agccaccatg accctattcc gacacgcttt
     3061 tggctgcgca caggaggcgt cggcgggtgc cgtcgtcgcg accgaccgcg tgtcgaccga
     3121 cgccgatcgc gacaacggga ccgtccttca cagtgcagct caattccgaa ggagttatga
     3181 ccttgcggtt aagcgcttag gtgcgcaaga agggaacgag cggcgaccgc cccgcttcct
     3241 ttcgctatga ctcagtagga atacaccggg tctggatcac aacaccgagt accgcctcaa
     3301 cgacgcaact atcgaagtcg acctgtcgga ggtcgaggcc gtagaccaga gtatgccctt
     3361 gagggtaacg tcaggatgcg ccatgcgtgg tgcctccttg tcgtgatccg aagcaaatac
     3421 ctacctcctt cgcctcgctc ccaaacgttt tccccaacgc tgtggtcccc ggacggctac
     3481 aacgctcgac ctatcagtga agcctaatgg gccgcctcga ctgcgaggcc cgtgaggaag
     3541 cggag
//
LOCUS       DASTKM010000325         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_670387, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000325 DASTKM010000000
VERSION     DASTKM010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670387"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFQ10_12800"
     CDS             <1..614
                     /locus_tag="VFQ10_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966266.1"
                     /translation="GGIERQQLKWFALAAALGASFLVGQQLIWLCAILIAYALGGEML
                     SLSRSLGNLLEVAINVCLYAAVGIAMLRYRLYDIDIIINRTLVYGALTVSLALVYFGG
                     VLSLQGLLRALTGQESQLAIVASTLAIAALFNPLHRRIQASIDRLFYRRKYDAAKTLD
                     EFSSKLRDETDLDALNSDLVKVVRDTMQPQHVTLWLREPKEPR"
     gene            611..1801
                     /locus_tag="VFQ10_12805"
     CDS             611..1801
                     /locus_tag="VFQ10_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966267.1"
                     /translation="MRARILARGLWVVTISLVAGSLVLGLANRCEAPLYEVTSAIIAP
                     TFATLGALISSRRPGNVIGWIFLAASVLVGVAMFSGQYATVALAPDGPTLSGGAVAAW
                     FAAQAQNAYLVFILFLVLLFPDGKLLSRRWRPMVWAIGAFIGGSLVIGALSPGPFVDF
                     PSASNPFGVQAIAPALEVLGVVGGLGGLICVAATIFSLILRFYRSRGEQRLQLKWFTY
                     ATTVGLLTPLILSEIAPAVFQVLGRFLWTLGFLSIPVSAAVAILKYRLYDIDRIINRT
                     LVYGALTVTLVAVYVGSVLSIQYAFRGLTGSESQLAIVASTLLIAALFNPLRRRVQNF
                     IDHSFYRRKYDAARTLEVFSAKLRDERDIDTLNSELLSTVRETMQPEHVSLWLREPER
                     KVER"
     gene            1971..2105
                     /locus_tag="VFQ10_12810"
     CDS             1971..2105
                     /locus_tag="VFQ10_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966268.1"
                     /translation="MSALRVFGIAFVVFTALAVHAARGFEDVLRVEAAEGSRLQNLVH
                     "
     gene            complement(2447..2956)
                     /locus_tag="VFQ10_12815"
     CDS             complement(2447..2956)
                     /locus_tag="VFQ10_12815"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9966269.1"
                     /translation="MLVTHDTEIEVLLGRLTDEILHNSNDSLVGLYVYGSLVTGDFDK
                     DRSDIDLLAVVDSDVDGDTFDRLDRMHARFVEDHPAWEDRIEVAYVPAPALWNFRTRT
                     DQIAVVSPGEPFHLKAAGKDWLINWYMVREVGVTLCGPPPSAMIPEISQSEFVEAVRE
                     QAYSRALLG"
     gene            complement(3062..3475)
                     /locus_tag="VFQ10_12820"
     CDS             complement(3062..3475)
                     /locus_tag="VFQ10_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9966270.1"
                     /translation="MDRLFGEMLGPLGRGRGRQGGGQLAQWAPAVDVLTKDGDLLIRC
                     ELPGVKQEDVDITLQNGVLTISGEHKVDEEEDRGGYHVRERRYGSFRRSMTLPEGVDE
                     SKIHARFEDGVLELSVEGAAVEQAPKRIEIEGSGG"
     gene            3706..>3986
                     /locus_tag="VFQ10_12825"
     CDS             3706..>3986
                     /locus_tag="VFQ10_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9966271.1"
                     /translation="MADVGERLENIRRLRMWTQVRLAREAGVSPTTVSAIETGKIGRP
                     HFGTLDKLARALRVAPQELQGSRAATIEGPRPLSMGWARDSEEGEFERGL"
BASE COUNT          713 a         1262 c         1172 g          839 t
ORIGIN      
        1 cgggcgggat agaacgacaa caactgaagt ggttcgcatt ggccgccgcc ctgggagcct
       61 cctttctagt cgggcagcag ctaatttggt tgtgcgccat cctgatagcc tacgctcttg
      121 gcggggaaat gctgagcctg agccgatcgc tggggaacct gctggaggtc gctatcaatg
      181 tgtgcctcta cgcggcggtc ggtatagcca tgctgaggta ccgtctctac gacattgaca
      241 tcatcatcaa ccgcactcta gtctacggcg ccctcacggt gtctttggcg ctggtctact
      301 ttggcggtgt gctctcgctc cagggtctgc tacgcgccct cacaggccag gaatcgcaac
      361 tggctatcgt ggcctccacg ctggctatag ccgcgctgtt caatccactg caccggcgca
      421 ttcaggcgtc tatagatcgg ctgttctacc gcaggaagta cgatgcggcg aagacgctcg
      481 acgagttctc ctcgaagtta cgcgacgaga cggacctcga cgcgttgaac agcgaccttg
      541 taaaggtggt gcgggataca atgcagccgc agcacgtcac cttgtggctc cgggaaccga
      601 aggagccacg atgagggccc gtattctcgc ccggggatta tgggtggtaa cgatctcgct
      661 cgtggccggc tctctcgtac ttggtctggc caaccgttgc gaggcccctc tctacgaggt
      721 cacttcggcg atcatcgccc ccaccttcgc cactctgggc gccctgatct cctcccgccg
      781 tccaggcaac gttattggtt ggatcttcct cgccgccagc gtcctggtcg gcgtggctat
      841 gttctcaggg cagtacgcga ccgtggcgct cgcccctgac ggcccgacgc tgtccggggg
      901 cgctgtcgca gcgtggttcg ccgcccaggc ccagaacgcg tacttggtct ttatcttgtt
      961 cctggttttg ctgttccccg acggaaagct gctctcgcgc cgctggcgac cgatggtctg
     1021 ggccatcggg gcgttcatcg ggggctctct ggtcatcggc gcgttgagcc ctggtccctt
     1081 cgtggacttc ccatccgcca gcaacccgtt cggcgtccag gccatcgctc cggccctcga
     1141 ggtcctaggg gtcgtcggcg gcctcggcgg gctgatctgc gtcgccgcga cgatcttctc
     1201 cctgatcttg cgtttctatc gctcccgtgg cgagcaaagg ctgcagctca agtggttcac
     1261 ctacgctacc acggtggggt tactgacgcc cctgatcctc agcgagatcg cccccgcggt
     1321 cttccaggtg ctcggtcgat tcctctggac gctcggcttt ctgagcatcc ccgtctccgc
     1381 cgccgtcgcg atcctcaagt accgcctcta cgacatagac cgcatcatca accgcaccct
     1441 ggtctacggg gctctgacgg tgacgctggt agcggtctac gtgggcagcg tattgtccat
     1501 ccagtacgcg ttccgcgggc tcacgggaag cgagtcgcaa ctagccatcg tggcatccac
     1561 tctcttgata gcggcgcttt tcaatcccct gaggcggcgc gtccagaact tcatcgacca
     1621 cagcttctat cgccgcaagt atgacgccgc cagaacgttg gaagtgttct ccgcgaagct
     1681 gcgcgacgag agggacatag acactctgaa ctcagaactg ctctcgaccg tgcgggagac
     1741 gatgcagcct gagcacgtct ctttgtggct gagggagccg gagagaaagg tggagcgatg
     1801 ataaccggga ttcttcgccg agcacctctc tttgccgggc ttgcggagga gcagctggag
     1861 taccatgccg acaatggggt gagagcgacc ctccagggca caccgattct aaagccacct
     1921 caagacttcg cagatctcag gccgtcgtgg ttgtgttaca cggaatccgc atgagcgctc
     1981 tacgagtctt tggcatagcg ttcgtcgtgt tcacggcgct ggcggtccat gcggcgcgcg
     2041 gcttcgagga cgtcctgagg gtagaggctg cggagggctc ccgtctccag aacctggtcc
     2101 actgagtgct cggggatcat catccactca gccgcatgcg ggagaggcaa tggtctattg
     2161 atggtgcgat acaagatcca gactacccac cgtcccggac gcgaacctga catgaatctc
     2221 aagtgtgcac ctcccgcaag atgttggatg aagggattat tctagcgtga aaggagggta
     2281 gactcggaca ccggcctgct tgcggcggcc ttcgcacaac cgtatcaccc cgaggcgtcg
     2341 gaggcgcttt ccacggtacc ggtgatccgg ctggcaacgt cgtgcacgaa acgcaccgtt
     2401 tcttggaggc cagcctcatc gcccgcttcg tggtcctggc cctcgctcag cctaagagag
     2461 ctctgctgta tgcctgttct cgcaccgcct cgacgaactc ggactgcgag atctccggga
     2521 tcatggcact gggcggcggg ccgcagaggg tgacgcctac ttcgcggacc atgtaccagt
     2581 tgatgagcca atcttttccg gctgccttga ggtggaacgg ttcgcccggg ctgacgaccg
     2641 cgatctggtc tgtccgggtc ctgaagttcc acagcgcagg ggcaggcacg tacgctactt
     2701 cgatgcggtc ttcccacgca ggatggtctt ccacaaagcg tgcgtgcatc ctgtccaaac
     2761 ggtcgaaagt gtcaccgtcg acatcggagt cgacgaccgc gagcaggtcg atgtcgctcc
     2821 tgtctttatc gaagtccccg gtaacaagag agccgtacac atacaagccg accagcgaat
     2881 cgttggagtt gtgcagaatc tcgtcggtca agcgacccag cagcacttcg atctcggtat
     2941 catgtgtgac cagcacggtc caagtgtagt cgactctctc cctctactcg cttccggaga
     3001 tggtcgctcg ccgggggggc cggctattgg ccggccctcc tccccctact caggcgaggc
     3061 cttagccgcc cgagccctct atctcgatgc gctttggggc ctgttcgaca gcggctccct
     3121 ccacgctgag ctccagcaca ccgtcctcaa agcgggcgtg gatcttgctc tcgtccactc
     3181 cctctggcaa ggtcatgctg cgacggaagg agccatagcg gcgctccctg acatggtagc
     3241 ctccacgatc ctcttcctca tcgaccttgt gctcacctga gatggtcaac accccattct
     3301 gaagagtgat gtccacatcc tcctgcttga ccccaggaag ctcgcaacgg atcagcagat
     3361 ccccatcctt ggtgagcacg tccacagccg gcgcccactg ggcgagctgc cctcctccct
     3421 gacgcccccg tccgcgacca agagggccca gcatctcgcc aaagaggcga tccacctcac
     3481 tctgagcatc gtaaaaccca cggaatggac tcaacatgtt ccttgcacct ccttctctgt
     3541 ctcgccccgt caacccggga ccaaactccc ttctcgtgga cactatacac catacaacac
     3601 agttgtcaac cattttttca ccagattttg gtgaaaaaaa cagctaacaa tggtctttta
     3661 cacagtttat ggtatgctgt ggcaaactaa gagtgaggag cgaacatggc tgatgtaggc
     3721 gagcgtttgg agaacatccg tcgcttgcgg atgtggaccc aggtgaggtt ggccagggag
     3781 gctggcgtga gtcccactac cgtctctgcg atcgagactg gcaagatcgg gcgtccgcac
     3841 tttggcacgc ttgacaagct ggctcgagct cttcgcgtgg cgccgcaaga gctccagggg
     3901 tcccgggcgg ctactattga gggaccgagg cccctatcga tggggtgggc tagagacagc
     3961 gaggagggcg agttcgagcg tgggct
//
LOCUS       DASTKM010000326         7414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1551218, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000326 DASTKM010000000
VERSION     DASTKM010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551218"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /gene="nusB"
                     /locus_tag="VFQ10_12830"
     CDS             complement(<1..315)
                     /gene="nusB"
                     /locus_tag="VFQ10_12830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET9966272.1"
                     /translation="MSRRTARKQAFLNLYQSDVNDCPLGPTMERWREYRGNLQEYAVT
                     LARGVEEEQEQLDALLSEVSVGWPVHRMSAVDRSILRLALYEMLFVDDVPPEVAVNEA
                     VEL"
     gene            complement(339..896)
                     /gene="efp"
                     /locus_tag="VFQ10_12835"
     CDS             complement(339..896)
                     /gene="efp"
                     /locus_tag="VFQ10_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET9966273.1"
                     /translation="MISTNQFRNGTAIRVDGKRFTILFFQHVKPGKGGAFVRTRLRNI
                     DSGAVVEKTFRAGEKLENVRTESRPMTYLYRDGDLLYFMDSETYDQVPVPTGVVGEAA
                     WYIVEGGTVSVLSADGEVVSVEPPAHVDLGVSETDPGLKGDTATGGTKPATLETGVVV
                     QVPLFINVGDQVRVDTRSNEYLTRV"
     gene            complement(1066..1491)
                     /locus_tag="VFQ10_12840"
     CDS             complement(1066..1491)
                     /locus_tag="VFQ10_12840"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET9966274.1"
                     /translation="MSKVFVLNGVNLSTLGTRRPEIYGTMTLDDIERLLRDEFPALDL
                     EFRHTDYEGEMVAFIKEAAGSDGLVINAGAWTHYSYALHDALESLDAPKVEVHLSNVY
                     TREEWRRESVISPAVDAVIAGMGPHGYAAAVRYVLGWGT"
     gene            complement(1484..2569)
                     /gene="aroB"
                     /locus_tag="VFQ10_12845"
     CDS             complement(1484..2569)
                     /gene="aroB"
                     /locus_tag="VFQ10_12845"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET9966275.1"
                     /translation="MPVETVAVPVTHPYRVFIGAGIDLAEIFSRSLEPGLCAIFTDST
                     VGPLHAARVQGALQRAGWRVAGKIEVPSGESSKSMEVYAAALGRLARMGLTRDGTIFA
                     LGGGVVGDLGGFVAGTYMRGIDLVMLPTSLLAMVDSAVGGKVGVDLPEGKNLVGAFVR
                     PRLVAADLEWVATLPEREISNGLAETIKMGLLSGGAFFGDLGLIGAARAGDLDALQTL
                     VLDSVSFKARVVAEDELEGGRRAILNYGHTIGHALEAAADYKLQHGQAVAAGMIAAAS
                     LSRERFGRDLTGVHEDLIGTAGLPHRVPDIGVERTLLAIARDKKRSAADGPAWHRFVL
                     LEDIGRPVRRVPVSEEEVREAIGAIVE"
     gene            complement(2551..3054)
                     /locus_tag="VFQ10_12850"
     CDS             complement(2551..3054)
                     /locus_tag="VFQ10_12850"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET9966276.1"
                     /translation="MDGSVAIVGYMGSGKTTLGRLLARAMSLEFVDLDRAIVKRAGKG
                     IPEIFAEHGEKHFRDLEHRALLQALDGRTPSVVACGGGTVVRPDNRILLREVTTVFLR
                     EDTDVLYRRTRGAGRPLRAASREEFGLRYAQRLPLYKEVADLEVEADGRPAAKVAEEV
                     LRCLSRR"
     gene            complement(3058..4221)
                     /gene="aroC"
                     /locus_tag="VFQ10_12855"
     CDS             complement(3058..4221)
                     /gene="aroC"
                     /locus_tag="VFQ10_12855"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET9966277.1"
                     /translation="MRFGFSTAGESHGPAEVALVHGVPAGLRLTAADVNRDLSRRQKG
                     YGRGGRQKIEKDEVEFLGGVRHGYTLGSPVAMLIRNRDYANWEHRMTPEPVEDPPQKI
                     TLPRPGHADLAGMQKYDFDDLRNVLERSSARETTARVAAGGVAKKLLAEFGVAIESAV
                     YRIGAVAYPKEQAVLDAATSDESEVRCPDPDVSERMKAEIDAARYARDALGGEFVVVA
                     RGCPPGLGSYVDWRDKLDARLAAAMMSINAIKGVEFGMGFGLAARRSSEVQDEILSEN
                     GRLARNSNRLGGFEGGMTNGEPVVVAAAMKPISTIAKALRTVDLATGEPTRAFKERAD
                     SCAVPAAAVIGESMAAIVLAEAFLEKFGMDNMTDIRAAYDAYTERVASTGNRR"
     gene            complement(4386..5069)
                     /locus_tag="VFQ10_12860"
     CDS             complement(4386..5069)
                     /locus_tag="VFQ10_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966278.1"
                     /translation="MASLRRVGHRLANLARGLVDTFLRDNRLLPPVLALLALFVFAWV
                     LAGVFLGRTDEQKPVAHRSEIAQADGAGGSDPAAPEIDNPNVDSYAAYRSKDPFREII
                     AKDTTIPEGTASVPAEQPTGSGGANGGRRGNDGNRRAGDSDRDGLPDRKEAILALDPN
                     NPDTDGDGIPDGLDDADGDGVPDGVSGGTVAGGGNNRSGGSGGGRGAGGRPGRGGDLL
                     DSGGTLSPP"
     gene            complement(5062..5772)
                     /gene="pilO"
                     /locus_tag="VFQ10_12865"
     CDS             complement(5062..5772)
                     /gene="pilO"
                     /locus_tag="VFQ10_12865"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET9966279.1"
                     /translation="MSRNDRNILILGILGIVIILVGYYFLLLSPLLQRLDEQAQARED
                     KQAQLEQVQQEVNELEEVRRNSPEIQSQLLELSKRVPTQPQIPTLVVQVEEIADASGV
                     TQLSVDPEPPTAPAGGGDYQAVPVTMTFDGTYDQMQDFLLRTRNLTRLVTVTDLSYCR
                     NPTIGEEASCEIEGEASGATDETTTLNPAVEARLSVEVQADVYFQPEGVPSGAETTAP
                     TPQETTTPSPPAEGTGGG"
     gene            complement(5769..6527)
                     /locus_tag="VFQ10_12870"
     CDS             complement(5769..6527)
                     /locus_tag="VFQ10_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966280.1"
                     /translation="MRRINLLPADERGRPADRLRRGVIGILLVVGAFVLILMAGVYLV
                     LLLRLNDLEDQVAKLDAEIARQNAHLAELSPYRDLQARLEAKKPVADGIYRSRFTWDQ
                     FLQGLAFVMPGTTALDTFTAEAAPVDIDAPVEQPLEPPGTISFTGIALPRYKNVADFV
                     VRMDNLQYLANADLEIATGQSNSRVSPVSFEVASELVTIVGESGTEVRLEGASQDELA
                     ARPGSMRTREIAKMKRPYGAAGEQYARHAGRGHR"
     gene            complement(6524..>7414)
                     /gene="pilM"
                     /locus_tag="VFQ10_12875"
     CDS             complement(6524..>7414)
                     /gene="pilM"
                     /locus_tag="VFQ10_12875"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET9966281.1"
                     /translation="KAVTLGVSNQKVVVRLLDFPHMAQADLQGAISFEAQDHIPMPLD
                     EAVLDYVVLGPREERSDLDRVLVVAAQREMISRYTSAVRTGGLRPAGVDVKALSLTRS
                     TLPDPFFGDEGAVLLLDVGAEITNLVVADRGNPVLTRFVPVGLVDFVAAVVELADLPE
                     DEAERLTLDPRVRLGSEQDEAQEQEGDEGNFDPALLYDVRRGLEGAAQTLADEVQRSI
                     EHHRSQEAAREVSRVLISGEAALVANLDGYLGELLDVPTSRGNPVEKLSANRSNISDD
                     QLRAMEPVLAVAFGLAMEEE"
BASE COUNT         1271 a         2464 c         2262 g         1417 t
ORIGIN      
        1 cagctcaacg gcttcgttta ccgcgacttc gggggggacg tcgtccacga agagcatctc
       61 gtagagcgcg aggcgaagga tcgaacggtc caccgcgctc atgcggtgca ccggccaccc
      121 taccgagact tcggagagca aggcgtcgag ctgttcctgc tcctcctcca cgccgcgggc
      181 aagggtgacc gcatactcct ggagattgcc cctatactcc cgccaacgct ccatggtcgg
      241 gccgagagga cagtcgttta cgtcgctctg atacaggttc aagaacgcct gcttgcgggc
      301 cgtccgccgg ctcaagggcc tctccagtcc accacgcttc agaccctcgt caggtactcg
      361 ttggagcggg tgtccaccct tacctgatcg cccacgttga taaagagcgg cacctgaact
      421 acgacccctg tctccagggt ggccggcttg gtcccccctg tcgccgtgtc gcccttgagg
      481 ccgggatccg tctccgagac cccgaggtcc acgtgggcag gcggctctac gcttacgacc
      541 tcaccgtcgg ccgaaagcac agagacggtg ccgccttcaa cgatgtacca tgccgcctcg
      601 ccgacgacgc ctgtgggcac cggcacctga tcgtaggtct ccgagtccat aaagtagagc
      661 aggtccccat ccctgtagag gtatgtcatc ggccgcgact cggtcctcac gttctcgagc
      721 ttctcccccg cacgaaaggt cttctcgacc acggccccac tgtctatgtt cctgagccgc
      781 gtccttacga acgctccgcc cttgcccggc ttgacgtgct ggaaaaacag gatcgtgaac
      841 cgcttgccat cgacccggat ggccgtaccg ttcctgaact ggttggtgga tatcatggtc
      901 ggaagctccc gttcacggcg cctgatcttc tcagcggacc tgcgcggcgt aaacccggcg
      961 cagacgcggc gatattatcg cacagtttct ttgcctgtca gcctggagcc gcgctgggat
     1021 aatagcaccg aaggagagcc tctcatagcg gagggcggac tctgttcaag tcccccaccc
     1081 gagaacgtat ctgaccgcgg cggcataacc gtgggggccc atgcccgcga tcacggcgtc
     1141 caccgccggt gagatgaccg actcccggcg ccactcctcc ctggtgtaca cgttggacag
     1201 atgaacctcg actttcggtg catccaggga ttccagggcg tcatgcagag cgtaagagta
     1261 atgggtccag gcaccggcgt ttatgacgag cccgtcggag cccgccgctt cctttatgaa
     1321 agcgaccatt tcgccctcgt agtccgtatg cctgaactcc aggtctagag ccggaaactc
     1381 atcccttagg agcctctcta tgtcgtcgag ggtcattgtt ccgtagattt cggggcggcg
     1441 ggtgccgagg gtgctgaggt tcacgccgtt gagaacgaag accttactca acgatcgctc
     1501 cgattgcctc gcgaacttct tcctcgctca caggcaccct ccgtacgggt ctaccgatgt
     1561 cttcgaggag cacgaatctg tgccaggcgg gtccgtctgc ggcggagcgt ttcttgtcgc
     1621 gggcaatcgc caggagcgta cgctcgacac caatgtcggg cacgcgatgg ggcaaaccgg
     1681 cggttcctat gaggtcttcg tgcacaccgg tcagatctcg gccgaaccgc tcgcgggaga
     1741 ggctggcggc tgcgatcatt ccggccgcaa cggcctggcc gtgctgcagt ttgtagtccg
     1801 ccgcggcctc gagggcgtgg ccgatggtgt gaccgtagtt caggatcgcg cgccgcccgc
     1861 cttccagctc gtcttcggcg accacgcgcg ccttgaagct cacggaatcg aggaccagtg
     1921 tctgcagcgc gtcgaggtca cctgcgcggg cggccccgat caagccgagg tcgccgaaga
     1981 acgctcctcc tgagagcagg cccatcttga tcgtctcggc gaggccgttg gagatctcac
     2041 gctccggcag cgtggcgacc cactccaggt ctgccgccac cagacggggc ctgacgaagg
     2101 cgccgaccag gttcttgcct tcagggaggt ctactccgac cttgccaccg acggcgctgt
     2161 ccaccatggc gagaagagat gttggcagca tgacgaggtc tatgccccgc atgtaggtgc
     2221 ccgcgacgaa gccgcccagg tccccgacca ccccgccacc gagggcgaag atggtaccgt
     2281 cgcgggtcag tcccatgcgg gccaggcgcc cgagagcagc ggcgtagacc tccatgctct
     2341 tggaggattc gccggaggga acctcgatct tccctgcgac acgccagcct gctctctgta
     2401 gggctccttg cacccgcgcc gcgtgcagcg gtcctaccgt cgagtcggtg aagatagcgc
     2461 agagaccagg ttcgagcgac ctggagaaga tctccgcgag gtcgatgccg gcgccgatga
     2521 agaccctgta cggatgggtg acagggaccg ctaccgtctc gacaggcacc tcagcacctc
     2581 ctccgccacc ttcgccgcag ggcgcccatc ggcctcgacc tccagatcgg cgacctcctt
     2641 gtagagaggg agacgttgtg cgtagcgcag cccgaactct tcgcggctcg ccgcccgtag
     2701 ggggcggccc gcaccacgag tcctgcggta gaggacgtcc gtatcttccc tcaagaagac
     2761 cgtcgtcacc tcgcgcagga ggattctgtt gtcgggacga actaccgtac caccgccgca
     2821 ggcgaccacg ctcggcgttc taccgtcgag ggcttggagt agggccctgt gctccaggtc
     2881 cctgaaatgc ttctcgccgt gttccgcaaa gatctccggt atccccttgc ccgcccgctt
     2941 tacgatggca cgatcgagat ccacgaactc gaggctcatg gctctggcca gcaggcggcc
     3001 cagggtggtc ttgccgctac ccatgtaccc gacgatggcc acggacccgt ccatgcttca
     3061 gcgtctgttc cctgtgctcg cgacacgttc ggtgtaagcg tcgtaggcgg cgcgaatatc
     3121 cgtcatgttg tccataccga acttttccag gaacgcctct gcgagcacga tcgcggccat
     3181 tgactccccg atcaccgccg ccgccggaac ggcgcaggag tcggcccttt ccttgaaagc
     3241 cctggtgggt tctccagtgg ccaggtctac tgtgcgaagg gctttcgcta tcgtcgagat
     3301 cggcttcatc gccgccgcga caacgaccgg ctccccgttc gtcatcccgc cttcgaatcc
     3361 cccgaggcgg ttgctgttgc gggcgagacg tccattctct gagagaatct cgtcctgcac
     3421 ctcgctggat ctcctggccg cgagcccgaa tcccatcccg aactcgacgc ctttgatcgc
     3481 attgatcgac atcatggctg cggcgagcct ggcatccagt ttgtcgcgcc agtcgacgta
     3541 ggagccgagg cccggcgggc aacccctggc cacgacgacg aactctcctc cgagggcgtc
     3601 gcgggcgtag cgggcggcgt cgatctccgc cttcatcctc tccgagacat ccgggtcggg
     3661 acagcgcacc tcggactcgt ctgaggtggc ggcatcgagt acggcctgtt ccttgggata
     3721 ggcgacggca cctattcgat agaccgcgct ttctatcgcg accccgaact cggccaggag
     3781 cttcttcgcc acgccgcctg ctgccacgcg ggccgtagtc tcgcgggcgc tggagcgttc
     3841 gaggacgttt cgcaggtcgt cgaagtcgta cttctgcatc ccggcaaggt cggcgtgacc
     3901 aggccttggg agtgtgatct tctgcggtgg atcctcgacc ggttccgggg tcatgcgatg
     3961 ttcccagttg gcgtagtccc tgttgcggat cagcatggca acgggcgagc ccagagtgta
     4021 cccgtggcgc acgccgccca ggaactcgac ctcgtctttc tctatcttct ggcgtccacc
     4081 cctcccgtaa cctttctgtc tgcgggaaag gtccctgttc acgtccgcgg cggtgagccg
     4141 gagacccgca gggaccccgt gcaccagggc gacctcggcg ggcccgtggg actctcccgc
     4201 cgtagaaaaa ccgaacctca caagcgcctc cgagccttta cgaccgacaa actcctgccg
     4261 ctaatataaa ccacgcgcat accacccaca cttaccgaag ctggaggttc gatcctgacg
     4321 cccatccggc ctcacgccgg atgctccgac aacgtaacga tcgcagaggt catgcccccg
     4381 ggccgctaag gtggagagag tgtcccaccg ctgtcgagca gatcaccgcc ccgtcctggc
     4441 cggccacccg cgccacggcc tcccccactt ccgcctgacc tgttattccc gccgcccgct
     4501 acggtgccac ccgagacacc gtccggcact ccgtcgccgt cggcgtcgtc gaggccgtcg
     4561 ggtataccgt cgccgtcggt atcagggttg ttcggatcga gggccaggat cgcctccttg
     4621 cggtcgggca aaccgtctct gtccgaatcc ccggcccgtc tattaccgtc attgccgcgg
     4681 cgacccccgt ttgctccccc tgaacccgtg ggctgttctg cgggaaccga ggccgtcccc
     4741 tccggaatcg tcgtgtcttt ggcgatgatc tccctgaacg ggtccttact gcggtaggcc
     4801 gcgtaggaat cgacgttggg gttgtctatc tcgggagcgg caggatcaga accccctgcg
     4861 ccgtccgcct gggcgatctc ggacctgtgc gcgaccggct tctgctcgtc ggttctaccg
     4921 aggaatacgc ctgccaggac ccaggcgaag acgaacaacg cgagcagggc cagaaccggc
     4981 ggcagcaacc tgttgtcacg caggaacgta tccacgagac cccgtgcgag gttggccagg
     5041 cggtggccga cccgccgcag gctagccacc gccggtcccc tcggcgggag gtgacggagt
     5101 ggtagtctcc tgtggggtcg gcgccgtggt ctcagcaccg gatggcacgc cctcaggctg
     5161 gaagtagacg tctgcctgta cctccaccga cagacgcgcc tccacggccg gattcaacgt
     5221 cgtggtctcg tctgtagccc ccgacgcctc gccctcgatt tcgcacgagg cctcttcacc
     5281 aatggtaggg ttgcgacagt agcttaaatc tgtcaccgtc actaacctgg ttaggttacg
     5341 agttctcaac aggaaatcct gcatctgatc ataggtccca tcgaatgtca ttgtaacggg
     5401 gacggcctgg taatcgccac ctccagcggg cgcggttgga ggttccgggt caaccgagag
     5461 ctgggtcaca ccggaggcgt ccgctatctc ctcgacctgg acgacgagcg taggaatctg
     5521 cggctgcgtg ggtactctct tgctgagttc gagaagctgg ctttggatct ccggggagtt
     5581 gcgcctgact tcctcaagct cgtttacctc ttgctgcacc tgctcaagct gtgcctgttt
     5641 gtcctcccgc gcctgggcct gttcgtcgag cctttgcagg agcggactca aaaggaggaa
     5701 gtagtagccg acgaggataa tcactattcc aagtatcccg aggatgagta tgttacgatc
     5761 gttgcgactc atcgatgccc acgtcccgca tgcctggcgt actgttcgcc tgccgccccg
     5821 tagggacgct tcattttggc gatctcgcgg gtacgcatcg agcccggtct ggccgcgagc
     5881 tcatcctgcg atgcaccttc caaccgtacc tcagtgccgc tctctccgac gatcgtgacc
     5941 agttccgagg cgacttcgaa gctcacaggg gagactcgtg agttcgattg cccggtggct
     6001 atttcgagat ccgcgttagc gagatactgg aggttgtcca tgcgcacgac gaagtctgcc
     6061 acattcttgt agcggggcaa cgcgatgcct gtaaacgaga tcgtgcccgg tggctccagg
     6121 ggctgctcga caggcgcgtc gatatcgacc ggtgcggcct cggcggtaaa cgtgtcgagg
     6181 gcggtggtcc caggcatgac gaaggccagg ccctgcaaga actgatccca ggtgaagcgg
     6241 ctgcggtaga tgccgtcggc gaccggcttc ttggcttcga gacgcgcctg gagatccctg
     6301 tacgggctca actcggccag gtgcgcgttc tgccgagcga tctcagcatc cagctttgcg
     6361 acctggtctt cgagatcgtt cagcctcaga agcagtacca ggtacacgcc ggccatcagg
     6421 atcagcacga aggcgccaac gacgagtagg ataccgataa caccgcggcg taacctgtct
     6481 gcggggcggc cccgctcgtc ggcaggaagg aggtttattc gcctcattct tcctccatgg
     6541 cgagaccaaa cgcgactgcg agtacgggct ccatcgctct caactggtcg tcggagatgt
     6601 tggagcggtt ggctgagagc ttctctacgg ggtttccgcg gcttgtcggg acgtccaaca
     6661 gttccccgag atacccgtca aggttggcta ccaaagccgc ttctccggag ataaggaccc
     6721 tggagacctc gcgggctgct tcctgagagc ggtggtgttc gatggatcgc tgcacctcgt
     6781 cggcgagggt ctgcgcggcg ccttcgagcc cgcgtctcac gtcgtagagg agcgccggat
     6841 cgaaattacc ttcatcacct tcttgttcct gggcctcgtc ctgctccgaa cccaacctga
     6901 cccgcggatc gagcgtcaac ctctcggctt catcctcagg aaggtcagcg agctcgacta
     6961 cagcggccac gaaatcgacc aggccgacgg gcacaaagcg ggtaaggacg ggattgccgc
     7021 gatcggccac cacgagattg gtgatctccg ccccgacgtc gaggaggagt acggctcctt
     7081 catcgccgaa aaagggatcg gggagggtgg agcgcgtcag cgagagcgcc ttgacgtcca
     7141 cgccggcagg ccgcaggcca ccggtccgga ccgccgaggt gtaacgtgag atcatctccc
     7201 tctgggcggc gaccaccaga acgcggtcga gatccgagcg ctcctccctg ggaccgagca
     7261 cgacgtagtc caaaacggcc tcatcgagcg gcatcggtat gtggtcctgg gcctcgaagc
     7321 ttatggcgcc ctgcaggtcg gcttgcgcca tgtgcggaaa gtcgagcagc ctgaccacta
     7381 ctttctggtt ggagacgccg agggtgacgg cctt
//
LOCUS       DASTKM010000327        35731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1026161, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000327 DASTKM010000000
VERSION     DASTKM010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 35731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35731
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026161"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFQ10_12880"
     CDS             <1..312
                     /locus_tag="VFQ10_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET9966282.1"
                     /translation="NKMMQQVQQMQQQMQQAQEELANETVTASAGGGAVKATMTGSME
                     LVSIEIDPEVVDPEDVEMLQDMVLAAVNEAMTSSQELANKKLGGITGGLGDLGLNLPG
                     L"
     gene            320..919
                     /gene="recR"
                     /locus_tag="VFQ10_12885"
     CDS             320..919
                     /gene="recR"
                     /locus_tag="VFQ10_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET9966283.1"
                     /translation="MATYARPVERLITELSKLPSIGPRSAQRIAFHVIRGKKEDAIGL
                     AQALQEVKEKIKPCRRCFNLTEADECEICRDARRDGAVICVVEDPYDIGPIERTNEYR
                     GLYHVLGGALSPLDGIEPEDLRIAELLERVRNEGTSELVIATNPNTTGEATALFIAQE
                     VKDLPVRVTALASGLPVGGDLEYADEVTLGRAFAARREL"
     gene            1011..3293
                     /locus_tag="VFQ10_12890"
     CDS             1011..3293
                     /locus_tag="VFQ10_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9966284.1"
                     /translation="MTETQAKSSLAGMSRRAVEALGDTVASLPQREATLVNFPDLRPE
                     LSEALRKMGASRLYSHQLEAYERVRAGENVVVATATASGKSLCYKIPAFQNALGRAKS
                     RAVFLYPTKALAQDQLGKIKAFDLRGVHPATYDGDTPHALRADIRRRANILLTNPDML
                     NIGILPNHEPWADYLRNLEIVAVDEAHVLRGVFGSHVAAVLRRLRRVAMLHGGDPRFV
                     LTSATIANPQELAESLTGLPFTLVDSDGASTGERRVIFRNPPLLDKEKGERRSLLTEG
                     ALVFANLVSQGVRTIAFARTRKAAELIYRYAADRLGVEGARRISPYRAGYTPRERREI
                     EGRLFRGDLLGVVSTNALELGVDVGALDAVVCCGYPGSVASIWQQWGRAGRGKDPALA
                     VYIPGRDSLDQFLFENPPRVLGRRVEAARVTMENPYILGPHLLAAAHEAPLDADDEEY
                     FGPAYRSVAKAMMEDRALASSGGRLVYVGGDNPARNISLRSASSQTVLIADGEGELIG
                     TAEATRAPSELHPGATYLHRGNAYEVESLDLRSHRAVARRVPNRYYTRPRVETAVEVL
                     EEIEKRDLANGSVLHWGRVRTTDSVTYFKKVRVADDKEVGVYPLDLPDVILETQALWV
                     TLPPLPREARPSFESFGGALHAGEHGMIGLLPLFAMCDRADIGGLSTPVHRQSRLPTV
                     FVYDGYPGGVGISRRGFDAFESLARDTLGVITRCPCERGCPACIQSPKCGNWNEPLSK
                     DGAVSLLRYVLGQTARYPTL"
     gene            3308..3751
                     /locus_tag="VFQ10_12895"
     CDS             3308..3751
                     /locus_tag="VFQ10_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HET9966285.1"
                     /translation="MAVVGSGVAKDELYEKAHEVGRLIAERGATVVCGGLSGVMEAAA
                     RGATEAGGTAIGILPDEDRRRQNEYLTYSVATGAGQARNLAVVCSGDVVIAIGGEYGT
                     LSEIGLARKVGRPVVVLEGWDLGEHVSVASSPQGAVEEAFGLIGR"
     gene            3802..4497
                     /locus_tag="VFQ10_12900"
     CDS             3802..4497
                     /locus_tag="VFQ10_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9966286.1"
                     /translation="MDSGQVALVSGGNRGIGLEVCRQLAERSYTVVMGSRDDKQGRAA
                     AAGLPYGVIVRQLDVADPESVERLSRSIEEEFGRLDILVNNAGISNDEGQRGVDADLD
                     RVKESLEANLFGAWRLCEMAIPLMRRNGYGRIVNVSTGLAALEDMGGGSPGYRISKTA
                     LNALTRILASELRGSGILVNAVNPGWVQTDMGGSGATRSVEEGAEALVWAATLPNSGP
                     TGGFFQDRRPVPW"
     gene            complement(4521..6242)
                     /gene="araD"
                     /locus_tag="VFQ10_12905"
     CDS             complement(4521..6242)
                     /gene="araD"
                     /locus_tag="VFQ10_12905"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HET9966287.1"
                     /translation="MNRKRRAEELRSHRWYGVRDLRSFGHRSRTKQMGYAAEDYSGKP
                     VVGIINTWSDINPCHAHFRQRAEEVKRGVWQAGGFPVELPAMSLSEPFQKPSTMLYRN
                     FLAMETEELLRSYPIDGTVLMGGCDKTTPALLMGAITMNLPAIFVPAGPMLRGNWRGS
                     TLGSGSDVWKYWDEKRAGTIGECVWEEIEDGIARSPGHCMTMGTASTMTAVAEVLGMT
                     LPGASSIPAADSGHPRMASSSGRRIVEMVWDDLKPSDILDGHSYEDAVTAVMALGGST
                     NAVIHLIALARRSGVPMDLARFDEISRRVPVLANIRPGGEYLMEDFYYAGGLRGLLSR
                     LAEMLHLDRATVGGHAMGETLTGVYKDEVIRPLDDPLQYEGGLAVLRGNLAPDGCVIK
                     HSAADPGMLRHKGPAVVFDGYDDLARRIDDPNLEVDADSVIVLRGVGPIGAPGMPEWG
                     LLPIPKKLLERGVRDMVRISDARMSGTAYGTCVLHVAPESHVGGPLALVREGDEIELD
                     VPNRRLELKVGAGELSRRRTAWRPPQPPYGRGYGVIFARHATQANEGCDFDFLAEGPP
                     VAEPEIH"
     gene            6515..7417
                     /locus_tag="VFQ10_12910"
     CDS             6515..7417
                     /locus_tag="VFQ10_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9966288.1"
                     /translation="MEHTDTLAALSDGMADAVERVATSVVRVNGRRRRSGSGVVFAQN
                     KVLTASHVLEREEDLSVETVGGRTLTARFAGRDHSTDLAVLDVEGLDVDPAAPAEGEA
                     RVGQISLAVGSHSRGEGPRATLGVVSAVGGPVRTRRGPRLERYIQTDATPYPGFSGGP
                     LIDVRGNVLGILVSGWGRGAAFAIPADLAWRTAGTLSERGSVKRGYLGILSQPVRLPD
                     GQRLGLSQRGGLLVVGVEDGSPAGRGGLIVGDILATLDGQPVEDTDDLLVLLAGDRVG
                     RPVPVGVVRGGELAEIEITVGERG"
     gene            7429..8313
                     /locus_tag="VFQ10_12915"
     CDS             7429..8313
                     /locus_tag="VFQ10_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9966289.1"
                     /translation="MAELSSDSSAVRMIEDVQRSVVQVRSSGRGIGAGVIWPGDGLVL
                     TNHHVISGRRRRGNIRVALHDGRTLDADVVKSGRRLDLALLRLEGDHGDLPPAPVGDS
                     DALRVGELVYAIGHPWGRVGAVSAGIVGGVGEVRGQGRSSSTRYVRSDVALAPGNSGG
                     PLLNARGEVVAINAMIFGLMALSIPSNAAQAWVAGERRPRLGIGVLPVEVPVSLRREA
                     GPAGLVIAGVETGGAADRAGLLVGDVLLAIAGKPLGEAGTLLETVAHAGDVVSLRVMR
                     GGKIMVMEVRLEESGRAA"
     gene            8310..8936
                     /locus_tag="VFQ10_12920"
     CDS             8310..8936
                     /locus_tag="VFQ10_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9966290.1"
                     /translation="MTHTRVFVVAPTPTMRAGLRSMLAAVEAADLLVVGEAGLAAGPV
                     PPLSGADVVLVADEELLEETALSVSEDGTQSLLLLSEDEGTASRLRTLSLRGWGVVPP
                     DAPSEELGAAVAAVAQGLTVLPKTLAERLFDEPAAAVEELSEPLTARESEVLGLLGRG
                     LSNKMIARELHISEHTVKFHISSIYAKLGVSNRAEAVSQGARHGLISL"
     gene            9280..9891
                     /locus_tag="VFQ10_12925"
     CDS             9280..9891
                     /locus_tag="VFQ10_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966291.1"
                     /translation="MYQEPTRVIVTSDDASLPDGCHPRQVAGLVISFVNAFNSGDQAS
                     LSRIFFLSEGPSPPDFADRGYDPWSWYTVSEVDTEGRIESGFVTYDQGELLRYFAKRH
                     RKGEQLRLLKISLTRTGLLGKEENVGFVYVLNRTARDLEPNLGGPARIASGQGAINCT
                     NRRIFAWRMDMRAEDRRTAREASNWLCTDPPGWKPGKAVVACT"
     gene            complement(9927..11102)
                     /locus_tag="VFQ10_12930"
     CDS             complement(9927..11102)
                     /locus_tag="VFQ10_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET9966292.1"
                     /translation="MSNAITEALETIDSFPDYPEARSPLHFELPPELEAGEPPEARGL
                     RRDEVRLMVSYLEDNSVVHSTFGDLPEFLSAGDTLVVNTSGTMKAALPAERRGGTPLT
                     VHLSTRLPADLWVVELRSSSGDEPVLDGESGEIVSLPAGASMVLHTPYLSEKRQRDEV
                     PNRLWISTLNLPVNFNNYLERNGSPIKYRYVRESWPAVYYQTVYATEVGSAEMPSAGR
                     AFTPELITRLVANGVRVVPLVLHTGVASLEEDEPPYEEFYRVPPETAAAINAARTAGH
                     RVVAVGTTVVRALETVTDSDGSTHPGEGWTGVFITPERGIRSVDAMLTGLHEPRSTHL
                     SMLEALVGSVSECPLATSGAEHLEVAYSEALKRGYLWHEFGDLHLILPGRQGTGALA"
     gene            complement(11118..11846)
                     /locus_tag="VFQ10_12935"
     CDS             complement(11118..11846)
                     /locus_tag="VFQ10_12935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9966293.1"
                     /translation="MSGESSDRTAMITGASRGLGLALARTLAEDGWTLIIDARTEEAL
                     ESARAELSRYTHVIAISGDVTDPEHREDLAEAAREVGGVDALVNNASFLGPSPQPDLL
                     DYPLDVIEQVYRNNVISPLALIQALNNELKPQARIINVTSDAAVEPYEGWGGYGSSKA
                     ALEQLSNILAAENPGLRVYRVDPGDMRTQMQQEAFPDEDISDRPLPEESVPGFIELLT
                     GDLPSGRYEARELAARVGTTAADS"
     gene            complement(12033..12677)
                     /locus_tag="VFQ10_12940"
     CDS             complement(12033..12677)
                     /locus_tag="VFQ10_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9966294.1"
                     /translation="MDKVRLLVADDHPMLREGLVAVLGTQPDFEVVGEAADGEEVVRL
                     AAILDPDVILMDLEMPGTDGVEALERLRDAGSRARTVVFTAYDTDARILGSLRAGARG
                     YLLKGASRKEIFGAIRTVQAGGSLLEPAVADRLLERIEGEENVPSNRRLTSRELEVLA
                     LLARGLKNAEIAERLFISERTVKFHVGSILAKLEAKNRTEAARIAVRRGLVEAP"
     gene            complement(12670..13860)
                     /locus_tag="VFQ10_12945"
     CDS             complement(12670..13860)
                     /locus_tag="VFQ10_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET9966295.1"
                     /translation="MPDASEVRETRDLERRNHELSVLNEIARELNRSVELGEALGFTL
                     SQVAELLGLRTAWIWLIDDSSPEPYLAAARNLPPALADDPSRMDGSGYCYCLDTYKRG
                     DLEGAANVNVLTCSRLDGLVDGTGGLRYHASIPLYAGEKKLGVMNVASPQWRGLSPED
                     LQLLSTIGDLLAIAVERASLFDRSARLGAVEERNRLAREIHDTLAQGLTATGLQLESA
                     EALLDDESGAERAREALRRALSLTRSNLDEARRSVLDLRAAPLEGRSLPEALKTLVDR
                     WEAETDVATRFRAVNGSRPLPPRVEAALYRVCQEALTNIASHAGASRVTVRLVATLET
                     VQLIVSDDGHGFDPSSVGDDRHGIVGMRERVEMLGGSLETESGPGEGTRIDVTVPLEG
                     LQSG"
     gene            complement(13966..14631)
                     /locus_tag="VFQ10_12950"
     CDS             complement(13966..14631)
                     /locus_tag="VFQ10_12950"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET9966296.1"
                     /translation="MESLLDFIRSIPSSPSGVLGFVTNLLVAHGYLVILFGAALDNFG
                     LPASGDIVLLAGGLFANGGRLALPLVMLSGFAGAFISDNSVYWIGRIGGRPMIYRILK
                     MRFLHFLVNEKSLARVERYFEAHGGKTVFVGRFGPGLRSMTPLFAGVTRMKYYRFLPY
                     NLAAGVVWAVTYSLVGYVFGQYWEELLAIVRSLGYGAIGLVALVLLAYVLRRRRFRNK
                     RGH"
     regulatory      14708..14875
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            14866..15117
                     /locus_tag="VFQ10_12955"
     CDS             14866..15117
                     /locus_tag="VFQ10_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HET9966297.1"
                     /translation="MTPNRGEDPEKETSMEKILGRRYPSTLPLWSLLTLALLLVALFV
                     LLSASGALLAPLLGQAAGPFDYLHEFAHDGRHLLGVPCH"
     gene            15126..15830
                     /locus_tag="VFQ10_12960"
     CDS             15126..15830
                     /locus_tag="VFQ10_12960"
                     /inference="COORDINATES: protein motif:HMM:NF021039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HET9966298.1"
                     /translation="MVSAYLRRGMAAGLLAGLLAGLFAFLVGEPILDRAIALEEASAG
                     AHHEEVFSRPTQKIGLFFATGLFGVTVGGVFGIGYAFFRERLAAGSDFKRSIFLAGAI
                     FVGAFLIPYLKYPANPPSVGDPSTIGERTLAYFTLVALSLLAILLAWLAARTLRSRGV
                     GASRRRLTVGAGLVVVIGVLFLLLPAAPSADEFPSGTLWAFRLSSFGTQLAFWAGLGL
                     LFGGLCERARRKGVTA"
     gene            15827..17440
                     /locus_tag="VFQ10_12965"
     CDS             15827..17440
                     /locus_tag="VFQ10_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HET9966299.1"
                     /translation="MNGALLVAGTHSDAGKSVVVAGICRWLAREGVSVAPFKAQNMAL
                     NSFVTREGAEMGRAQASQAAAAMIEPEVAMNPILLKPSAERTTQVVVRGKVRANASAT
                     GYGGMKKTLMPVVLESLEELRRRFDVVVCEGAGSPAEINLRENDLANMGLARAADLPV
                     LLVGDIDRGGLFASLYGTLALLSPEDQSLVASFLVNKFRGDPAVLAPGLERMTSLTGR
                     PFLGTLPWIPGLGLDGEDSLALDAPRPVKPPLGRDVLSVAVARLGRISNFTDFDALVQ
                     EPGVSVRFTGLAEEILDADLAVLPGTKATVEDLKLLRSRGIDHAFAERVKRALPTLGI
                     CGGYQMLGRRIEDGVESGEAEVLGLGLLPVETSFEVEKMLGRPQGWALGFGGAEVAGY
                     EIHHGRVRREGGDPLFDTVEGTEGCRSGATLGTSWHGILESDGFRRALLLWVTEERGL
                     DWKPGEMPFAAAREAQLEKLGNLVADHVDREALLRLIDVGQPSGLPVIGLQARGYKPR
                     EGNGDGLLTGTAEPVGLPPNAPVNLKAET"
     gene            17440..21195
                     /gene="cobN"
                     /locus_tag="VFQ10_12970"
     CDS             17440..21195
                     /gene="cobN"
                     /locus_tag="VFQ10_12970"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HET9966300.1"
                     /translation="MPETSRSKLLFLTTADTEILAAARASERLPEGFPMLLCANPVVL
                     EDPAAFFERELPGAGAVLVRLLGGRKAWPEGLEELRRRCARLRVPLLAFGGEAEPDAQ
                     LTALSTVPSGTVLEAFEYLRYGGVRNTENLLRFVADTVLMEGYGFEPASPLPEVGVYH
                     PGLPEGSTGEELLARHDPARPTVGVIFYRAHWMSGNTAFVDALVEALEAAGADALPVY
                     CYSLRAGPDGQVQGLELLKERVDCLVTTVLAGGGSNAADARRSGSPQEWLEWDVPALE
                     ELGVPVIQGICTTSTREAWLASDAGLSPLDTAWQVAIPEFDGRNIGVPFSFKERLGED
                     SPVGAPLTLYKADPERTARLAGLAARFARLGRIPNSEKKVAVLLSNYPTKHSRVGNAV
                     GLDTPVSAIKLLDAMREAGYKVEGAPERGDELIHSLIGAGGHDLEFLTGDQLGNAAGR
                     LDAKRYAEWFGRLPEELRSDVEEHWGRPPGDLYVDGDEFVVAGLSFGNVFVGIQPPRG
                     FGENPIAIYHDPDLPPTHHYLAAYWWMIEEFGADAIVHLGKHGTLEWLPGKSLGLSPS
                     CAPDAALRDVPLFYPFVVNDPGEGTQAKRRAHATVVDHLIPPMTRAETYDDLAKLEQL
                     LDEYYQVETLDPSKLPAIRVMIWETLRNAELHRDLGVEEQPEEFSDFLTHVDGYLCEI
                     KDLPIRGGLHILGQTPDGESLRHLLASILRLGAGEVPGLRRAAGAAFGLDEKVLAENG
                     GTKVEAPPGLVERFPGTVVTASDLLDRLEEAQQALLSGMEERGWDAGSARAVCEKTLG
                     VADAGVERSLRFAAEEVVPRLMRTPEEMGNLLSGLGGGYVSAGPSGSPTRGLINVLPT
                     GRNFYSVDPKALPSALSWEVGQGLADDLLRRYLEEEGRYPETVGIVVWGTAAMRTQGD
                     DVSEILALLGVRPVWNEESRRVVGLEVIPPRELRRPRVDATVRISGFFRDAFPNLISL
                     LDEAFTTVAELEEPDHMNFVKKHAGEERSRGADERRSTTRIFGSKPGAYGAGLLPLMD
                     ARNWRTDEDLAEVYAVWGGYAYGRGLDGVEAREAMESNLRRTEVAVKNVDNREHDLFD
                     SDDYFQYHGGMIAAVRALTGSDPKAYIGDSADPSRVKTRDLSEEARRVFRSRVANPKW
                     IRAMQRHGYKGAFELSATVDYLFGYDATAGVVEDWMYSDVTRKYVLDQEARDFMQQSN
                     PWALRAISERLLEAAQRGLWSEPDTEDLEALKQVYLENEGMLEEGIS"
     gene            21192..23144
                     /locus_tag="VFQ10_12975"
     CDS             21192..23144
                     /locus_tag="VFQ10_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative cobaltochelatase"
                     /protein_id="HET9966301.1"
                     /translation="MNAYLHPYPFSAIVGQDDLKLALLVNAVSPEVGGVLVRGEKGTA
                     KSTAVRALASLLPSIRVISGCPFSCDPEAPNPECPAGPHPLDASAETRPVRLVELPVG
                     ASTDRLAGTLDIEKALSEGRKAFEPGLLAAAHRGILYVDEVNLLSDHLVDLLLDVAAM
                     GVNHVEREGVSVRHPSRFMLVGTMNPEEGELRPQLLDRFGITVEVAGSPDPADRVEVV
                     RRRLRYETAPQEFSGEWSSVDAKLAASVESARLRLPGVSLDEDILFRISTLCAELGVD
                     GLRGDLVTAKTARTLAAWDARNEVVLDDVKRAALLALSHRRRRGPFEQPGMDPEDLEN
                     VLSEDSPDPPDDPGGGSIRPATSENDREVSSSVRDPEPSEAHKGAGERSHAASEPFDP
                     VRLEVTEKGKGGPLGRRSRVSGETGHPVADRENGGRPRDVALAATVRAAAPHQRARGG
                     EGSGIEVRTCDLRQNVREGREGNLILFLVDASGSMAARKRMSAVKGAVLSLLNDAYQR
                     RDKVTLISFRGKGARILLPPTSSVGLATSRLEDLPTGGRTPLAAGIEKAAEVLKREEL
                     RDRERRPLLVLLTDGRSTAGPDPQVAAARMRSLGATSFVVDTEEGYVRLGMAGELAGA
                     MGARCLRLEELRADSLVELIERRRVA"
     gene            23141..23713
                     /gene="cobO"
                     /locus_tag="VFQ10_12980"
     CDS             23141..23713
                     /gene="cobO"
                     /locus_tag="VFQ10_12980"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET9966302.1"
                     /translation="MSETREPREQRLRRRSNRERPLLIVNTGHGKGKSTASFGVMLRG
                     WARGYRIGVYQFVKSGKWNVGEQKAAEALGNIDWFKQGDGWTWTVKDLEHSADLAREG
                     WEEVKRRLADETYDMLLLDEFTYPMKFGWVDTSEVVEVLKNRPGFQHVIITGRDAPPE
                     LVEISDLVSEVNKVKHPFDEGYRGQKGIEW"
     gene            23707..25068
                     /locus_tag="VFQ10_12985"
     CDS             23707..25068
                     /locus_tag="VFQ10_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HET9966303.1"
                     /translation="MVDTPRIVVAGTHSGVGKTTIASGLMAALSLEGFRVAPFKVGPD
                     FIDLSYHTLAAGRQGRNLDAFLSGPDLIGPLFAHGAQSADIAVIEGVMGLFDGKGGGD
                     LASTAHVARLLQAPIVLVVDARAMARSAAAMVHGYTTFDPKARVAGVVLNRVGSRVHE
                     SMLREALRPLGIPVLGVLRRDDDIQTPDRHLGLVPVAERREEATRSLHALGKVTSRSL
                     NLDGIVRLARSAGPLVVEPWSPESPDLDRSCGVRVAVAAGPSFSFLYRENLELLEGAG
                     AEVVTFDPTTEEDLPAGTDALYLGGGFPETYAKVLSTNGRMRESVRAFAAGGRPVVAE
                     CGGLLYLVRELDGHRMCGVLDAEATMTDRLALGYREARALADSPLAERGANVRGHEFH
                     YAAVETRGESRPAWDLIGRGKEGFVAGGVHASFLHTHWAAVPELPRRLVLEASLSATE
                     VGV"
     gene            25065..25778
                     /gene="cobI"
                     /locus_tag="VFQ10_12990"
     CDS             25065..25778
                     /gene="cobI"
                     /locus_tag="VFQ10_12990"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HET9966304.1"
                     /translation="MSGRLVGVGVGPGDPELLTVKGLGLLREADEVFVPVADTGEVGR
                     AEATVREYLDERRIRRLPFALSDDAEARERNWTNAAHRVSEHLRSGKTCAFATVGDPN
                     VYSTFTYLARAVQEIEPDVEVETVPGITALQDLASRSGTVLLEGDERLALLPFTAGEE
                     PLREALAGFETVVCYKGGSRLGEVLRVAEEGGRLEGAVYGARLGIEGEEVVPALEMVG
                     REGTYLSTVIFSGRSSEFG"
     gene            25780..26559
                     /gene="cobM"
                     /locus_tag="VFQ10_12995"
     CDS             25780..26559
                     /gene="cobM"
                     /locus_tag="VFQ10_12995"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HET9966305.1"
                     /translation="MWFIGAGPGAPDLLTVRGARLIGEADVVVWARSLVQEGVLQYTR
                     PGAQIVESTTIPLEDVRGLYERAVDEDLRVARVHSGDPSLYGAVMEQIELCNEIGLDW
                     EIVPGVSSLGAAAAAIGRELTVPEVSQSVILTRRASRTPMPDGEDIRSFAEHGTTMAI
                     FLSAAKPRVLQQDLLEGGYAPETPCAVVYRASWPDEEVIECRLEDLADRVREAGFTRQ
                     ALILVGPGLGAQGTRSHLYSPAFSHMFRRAKPAEKGADAAT"
     gene            26546..27712
                     /locus_tag="VFQ10_13000"
     CDS             26546..27712
                     /locus_tag="VFQ10_13000"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HET9966306.1"
                     /translation="MPPRKLREPSMPPSMEKVKGQKLRTGWTTGTCAAAAAKAAASAL
                     ASGRPQTRVDVKLPGKGRERRVEFDVERCDLGGSWAEAVVIKDAGDDPDVTDGAHLTA
                     RVSWLERQGLELDRGDGVGSVTKPGLGLPVGGPAINAVPRRMISYSVEEVLDPDERGV
                     RVVISVPGGEKMAEKTTNARLGIVGGISILGTTGIVRPFSTAAWAASVVQAIDVMGAQ
                     GHKTFVLSTGGLTEKAAMRLLPDLEEVNFVEVGDFTGQAIKKAVENGLERCFFVGMAG
                     KISKLAAGVMMTHWTRSKVDNDLLAKITLEAGGSTNLVEEVKGANTARHAYELWRSVH
                     LDRGADLLCQQAAENLAEYAEGKLEVHVILVDFDTLEPAGASLGALTLTTWGPT"
     gene            27709..28917
                     /gene="cbiE"
                     /locus_tag="VFQ10_13005"
     CDS             27709..28917
                     /gene="cbiE"
                     /locus_tag="VFQ10_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HET9966307.1"
                     /translation="MSRGRITVVGLDGRPLDEEIARLLADAALVAGGRRHLEMLGVES
                     DRALVLEGDLSEALARIEATGGPVVVLASGDPGFFGIVRLLAGRFGRENLRIWPGLSS
                     VALAFARAGLSWDDAVTVSAHGRDPRRAVNVCRAHPKAAVLTSPDFGPAELAAALDGL
                     GRSFVVAEKLGGPHERIFNGDAGTVAGMVWEDPNVVLVLDEERIPGARGWISSSLQSP
                     LRWALPEGEFEHRSGMITKSATRAFVLSRLGPGPGDLIWDIGAGSGSVAIECARLGAA
                     AIAVESDPESCARIRRNAEHHGVRLQIMEATAPQVLWDLSEPDAVFVGGSGGAFEEIV
                     KLSAVRARRAVVLALVTLERVVPAAQLLEDCGLEAETTFLQASGMKGVGNLHRLAAES
                     AIFVVSGSRT"
     gene            28914..30599
                     /gene="cobJ"
                     /locus_tag="VFQ10_13010"
     CDS             28914..30599
                     /gene="cobJ"
                     /locus_tag="VFQ10_13010"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HET9966308.1"
                     /translation="MIGLIAATANGRRNAAHLAGTWPDTRLYEGKAKVALKRAWYECD
                     GIVLFLATGAALRLLAPLLASKQQDPGVVTVDDAGNFVVALCGGHEGGANALAARVAD
                     TLGGTPVITTASDSVGFPALDSLGDRLGLRLEGDSDLASVGAALVSGEKVNFVTDRRW
                     PLGPLPENVGRKDEWEAPLILISDRLLETPRPAVVFRPPSLVAGVGCSRGVDAEEILE
                     LLLRSLAQAELTEKSVAALASIDVKRDEAGMLQAAEKLDVPIHFHPAEALSVVEAPNP
                     SELVRDAVGTPSVAEAAVVSSGAELVLEKQKSANATVAIGRLPVRGRLALVSLGPGED
                     TLIPPLAREALVSSEFVVGLDQYVDRIRHLLRPGTRVLTPPLGNEVERAELALAEARA
                     GGSVALVSSGDVGIYAMASPGLELADEDVDVVVVPGVTAAQAAASLLGSPLGHDHCSV
                     SLSDLLTPWGVIQSRVRAAAEGDFVVSLYNPRSKGRDWQLGKVKEILLEHRPPDTPVG
                     IVKDAYRQTQRVILTDLASLRQEDVDMLTIVLVGSSQTRVVAGRMVTPRGYLS"
     gene            30596..30799
                     /locus_tag="VFQ10_13015"
     CDS             30596..30799
                     /locus_tag="VFQ10_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9966309.1"
                     /translation="MKAVFAVTEACAGCGACIKTCPEKAILPAPRDYGAPLLVLEDRC
                     SGCAECAEICPVSACIEVLLEAE"
     gene            30796..31380
                     /locus_tag="VFQ10_13020"
     CDS             30796..31380
                     /locus_tag="VFQ10_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HET9966310.1"
                     /translation="MRHVHPIETESYRILRGMVDLSYLGPLSRAVAERVIHASADLDY
                     AENLVLDETALERGISALRRDGPVVTDVGMVAAGITGRQTLCLISDPRAKDLSEQLGI
                     TRSAAGFRVAAEEVGKGAVWIVGNAPTALFELLGLDVDPDLVIGLPVGFVGAAESKEE
                     LVASGLPAVANRGPKGGSAVAAAAVNALIYYEEK"
     gene            31382..32302
                     /locus_tag="VFQ10_13025"
     CDS             31382..32302
                     /locus_tag="VFQ10_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HET9966311.1"
                     /translation="MRRPVGIEIRETYGDAPALLIVGHGSRDSRGAAEFHDLVDLVRR
                     RNPSLTVEGGFIELSRPPISECVDRLADRGARNAAAVPLMLLAAGHAKDDIPATLVRE
                     KMGHPEMSFNYGRALGIRPELLELMDERISTVVLEEEKGETAVLVVGRGSSDPDANSD
                     LSKIARLFYEGRPYPVVESAYVSMTQPDVAEGLDRCLKLGARHVVVFSYFLFTGVLEQ
                     RIRGQGEAFALANPGVELRYAGYFGPDELVADLVVERYTEAVEGDIRMNCDVCVHRVA
                     LPGFEEKVGAPATPHYHPDEPGRHQHGHHH"
     gene            32305..33366
                     /gene="cobT"
                     /locus_tag="VFQ10_13030"
     CDS             32305..33366
                     /gene="cobT"
                     /locus_tag="VFQ10_13030"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HET9966312.1"
                     /translation="MALEDRLLELAGEILPPDETAAEEARNRHLGLTKPPSSLGVLEE
                     LGARLAGMAGDCPPPVPESPAVVVCAGDHGVLDRGVSPWPREVTAAMVSNFCAGGAAV
                     NAIAKTVGARVSVLDVGVAGELERHPLLRAAKVRRGTGDLSRGPAMSREEAARAVMAG
                     AGVAEELVESGGVDLLVTGDMGIGNTTPAACLISAFAGSPPEETTGRGTGIDDQTLKL
                     KVNVVEEALELHSPDPEDPLGVLAQIGGLEHAAIVGVILTGAIYGIPVILDGVVSDSA
                     ALVACAIAPHSAGYVIAGHRSAEPGARIVLESLKLEPLLDLDMRLGEGTGGLLAVPLV
                     QAAARMLAEMATLEELGIG"
     gene            33359..34192
                     /locus_tag="VFQ10_13035"
     CDS             33359..34192
                     /locus_tag="VFQ10_13035"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET9966313.1"
                     /translation="MVEPLERGYIEALRSPLARVVEVHGALGSTQERARELAHAGTPH
                     GTLIVAEIQTGGRGRLGRPWGSPMGGLWISLVLRPEFAASLASRITQTASVGVAKALW
                     EVGVEARIKWPNDLLAHGKKICGILAESSAGNVTGPASERYLDYVILGVGMNANFDPA
                     ELAVPDREVTTIRYELGRDVNLLDLLRALLSNLETELGRIEDFGAVLEDWRNVNCTLG
                     ERVRILRLGETIEGRAVDLTAEGALLLVTRRGTVEVFEGEIEHLSQGVEVRSQLDPGP
                     T"
     gene            complement(34515..34769)
                     /locus_tag="VFQ10_13040"
     CDS             complement(34515..34769)
                     /locus_tag="VFQ10_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966314.1"
                     /translation="MAYFEGQLKRASTEAGEEHTGYVLETEIDMSAVQDADELIGDQI
                     AVTGDVEIVDFPERGKILIFRATSVAVLEEDEEDEEMPTA"
     gene            35079..>35731
                     /locus_tag="VFQ10_13045"
     CDS             35079..>35731
                     /locus_tag="VFQ10_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966315.1"
                     /translation="MNATLMYVGIGAVLATGVAILTIAVLFLQTARRYVDLTERRLEL
                     LREGEAFLLNLVQRQGQALEREAERREKAPEMVGNPRGRQQGEESTGSRRSEGLNQRV
                     QETPYTGSSSEVEDEEQRRERTPKPDPAPARPKDNAPRLGVQVPHPDDDVPGRGWNNS
                     PAKFFQKCYDRYLEHYEGYVRLAERLHQMRDEADAAPGSLGEWEDKLRRAYDAIERTT
                     "
BASE COUNT         6296 a        11109 c        12102 g         6224 t
ORIGIN      
        1 aacaagatga tgcagcaggt tcagcagatg cagcagcaga tgcagcaggc tcaggaggag
       61 ctcgcgaacg agacagttac cgcgagcgcc ggcggtgggg ctgtgaaggc gacgatgacc
      121 ggcagcatgg agctcgtctc catcgagata gaccctgagg tggtagatcc cgaagacgtc
      181 gagatgttgc aggatatggt gctggccgcc gtcaacgaag cgatgacgag ctcccaggag
      241 ctggccaaca agaagcttgg tgggatcacc ggcggcctcg gggacctggg cctgaacctt
      301 ccgggtctgt aagggctctt tggcgaccta cgcaaggccg gtagagaggc tcattacaga
      361 gctctccaaa ctgccctcca tcgggccgag gagcgcgcag cgcatcgcct tccacgtaat
      421 caggggtaag aaagaggacg ctatagggct cgcccaggcg ctgcaggagg tcaaggagaa
      481 gatcaaaccg tgcaggcgct gcttcaacct gaccgaggcc gatgagtgtg agatctgccg
      541 cgatgcccgt cgggacgggg ccgtgatctg cgtcgtggaa gacccctacg atatcggtcc
      601 aatagaacgg accaacgagt accgaggcct ctaccacgtc ctcggcgggg ccctgtctcc
      661 gttggacggg atcgaacccg aggacctgcg aatagccgag cttctggagc gggtccgaaa
      721 tgaaggcacc tcagagctgg tcatcgcgac caacccgaac accacgggcg aggcgaccgc
      781 actgtttatc gcgcaggagg tgaaggacct gcctgtcagg gttacggcgc tcgcgagcgg
      841 cctgcccgtc ggtggcgacc tcgagtacgc cgacgaagtg accctcggaa gggccttcgc
      901 cgcaaggcgt gagctgtagg cgatttttgg aagtccactt cccgaaacag cgccgtcagg
      961 cttcagctat cgacttttct ggttcgtgcc gatagctttg cagaaggttc atgactgaga
     1021 cacaggcgaa aagctctctg gccgggatga gccgcagggc ggtcgaggct cttggggata
     1081 ctgtggcgag cctcccgcag cgggaagcga cgcttgtgaa tttccccgac ctccggccgg
     1141 agttgtccga ggctttgcgt aagatgggcg cctccaggtt gtactcgcat cagttggagg
     1201 cctacgagcg ggtacgggcc ggtgagaacg tcgtcgtcgc gacggcgacg gcgagcggga
     1261 aatcgctatg ctacaagatc ccggccttcc agaacgcgct gggccgggcg aagagccgcg
     1321 ctgtcttcct ctatccgacg aaggctctgg cccaggacca gttaggcaag atcaaggcct
     1381 tcgacctgcg cggcgtccat cctgcgacct acgacggcga cacgccgcac gccctccgcg
     1441 cggacatcag gcgcagggcg aacatcctgc tgaccaaccc tgacatgctg aacatcggca
     1501 tcctgcccaa ccacgagcct tgggccgact acctgcgcaa cctggagata gtggccgtcg
     1561 acgaggcgca cgtcctgcgc ggcgtattcg ggtcgcacgt ggctgccgta ctgaggcggc
     1621 tgaggcgggt ggcgatgttg cacggtggag atccccgttt cgtactcacg agcgccacga
     1681 tagccaaccc gcaggagctc gccgagagtc tcactggcct gcctttcacc ctcgttgaca
     1741 gcgacggcgc ttccacgggc gagcggcggg tcatcttccg caaccctccc ttgctcgaca
     1801 aggagaaggg ggagaggcgg agtcttctga ccgaaggcgc tctggttttc gcgaacctgg
     1861 tctcgcaggg ggtcaggacc atagctttcg ccaggacgag gaaggctgcc gagctcatct
     1921 accgctacgc cgccgacagg ctcggcgtcg agggtgcgcg caggatctcc ccataccgcg
     1981 cgggctacac cccgagagag agaagagaga tcgagggccg actcttccgc ggcgaccttc
     2041 tcggcgtcgt ctccacgaac gcgctggagc tcggggtgga cgtgggggcg ctggacgccg
     2101 tagtctgctg cggctacccg ggcagcgtgg cctccatctg gcagcagtgg ggccgcgccg
     2161 gacgcggcaa ggaccccgcc ctcgccgtgt acatccccgg ccgcgactct ctcgatcagt
     2221 tcctcttcga gaaccctccg agagtcctcg gcaggagggt ggaggcggcc agggtcacga
     2281 tggagaaccc ctacatactc ggcccgcacc tgctcgccgc cgcccacgag gcaccgctcg
     2341 acgcggacga cgaggagtac ttcgggccgg cttacaggag cgtggcgaag gcgatgatgg
     2401 aggatagggc gctagcttcc agcggaggtc ggctggtcta cgtcggcggg gacaaccctg
     2461 cgcgcaacat ctctctgcgc tcggcatcat cccagacagt gctcatagcc gacggtgaag
     2521 gagagttgat cgggaccgcg gaagccaccc gcgccccgag cgagctgcat ccgggcgcga
     2581 cctacctcca ccgcgggaac gcatacgagg tggagagcct ggatctccgt tcccaccgcg
     2641 ccgtcgctag gcgcgtcccc aatcgttact acaccaggcc gcgcgtcgag accgccgtag
     2701 aggtcctgga ggagatcgag aagcgggacc tcgcgaatgg atctgtgctc cactggggca
     2761 gggttaggac gactgactcc gtcacctact tcaagaaggt aagggtggcc gacgacaagg
     2821 aggtcggggt ctaccctttg gatctccctg acgtgattct ggagacgcag gcactctggg
     2881 tcacgctgcc tccgcttccc agagaagcta gaccgagctt cgagagcttc gggggcgccc
     2941 tgcacgccgg ggagcacggt atgatcgggc tcctcccgct gttcgccatg tgcgaccgtg
     3001 cagatatcgg cggcctctcc acgcctgtcc atcgccagag ccgtctcccc acggtcttcg
     3061 tctacgacgg ctatcctggc ggcgtcggca tctctaggcg cggcttcgat gccttcgagt
     3121 cgctcgcccg tgatactctg ggcgtcatca cccgatgccc ttgcgaaaga ggatgccccg
     3181 cgtgcataca gtcccccaag tgcggcaact ggaacgagcc cctgagcaag gatggggcag
     3241 ttagtctcct caggtacgtg ctcggacaga cggcccgcta cccgaccctg tgagaggcac
     3301 gccttacgtg gccgttgttg gatcaggcgt agccaaggat gaactctacg agaaggcgca
     3361 cgaggtcggt aggctaatcg ccgagagggg cgcaaccgtc gtctgtggcg gactctcggg
     3421 cgtgatggag gccgcagccc ggggggccac cgaggccggt ggcacagcta taggcatcct
     3481 gcccgacgag gaccggcgga ggcagaacga gtatcttacg tactctgtgg ccacgggcgc
     3541 ggggcaggcg cggaacctcg ccgtggtgtg ctccggggac gtggtcatcg ccataggtgg
     3601 cgagtacggg acgctctctg agatagggct cgccagaaag gtcgggcgac cagtggtggt
     3661 cctggaaggt tgggatctcg gtgagcacgt gtcggtggcg tcgtcaccgc agggcgccgt
     3721 cgaggaggcg ttcgggctta tcggtagata ggaacgcgag cgaaatgagg tcggagatac
     3781 ataagatcgg gaggtcgaac aatggatagt gggcaggtcg cgcttgtaag cgggggaaac
     3841 cggggaatcg ggctggaggt ctgccgccag ctcgccgagc gtagctacac ggtcgtcatg
     3901 ggctcccgcg acgataaaca gggcagggcc gcagcagcgg gacttcctta cggcgtgatc
     3961 gtccggcaac ttgacgtagc agaccccgag agtgtcgaac ggctctcgcg ctccatcgag
     4021 gaggagtttg gacggctcga catcctggtg aacaatgccg gtatctccaa cgacgagggc
     4081 cagcgaggcg tggatgcgga cctcgaccgg gtgaaggaga gtctggaggc gaacctgttc
     4141 ggggcctggc gcctgtgcga gatggccatc ccactcatgc ggcgcaacgg ctacggacgc
     4201 atcgtcaatg tctcgacggg actcgctgcc ctggaagaca tgggaggcgg ctccccaggg
     4261 taccgcatct cgaagaccgc cctcaacgcc ctgacgcgca tcctcgcctc agagctgcgc
     4321 gggagcggca tcctggtcaa cgctgtcaac cctggatggg tgcagaccga tatgggtggc
     4381 tcgggcgcca cgcgctccgt cgaggaaggc gcagaagccc tcgtctgggc ggcgacgcta
     4441 ccaaacagcg gccctaccgg cggcttcttc caggaccgcc gcccggtacc ctggtgacca
     4501 cgctcgccac gccccagtgg tcagtggatc tcgggttctg ctacaggcgg tccctctgcg
     4561 aggaaatcga agtcgcatcc ttcgttggcc tgggtcgcgt gcctggcgaa gatgaccccg
     4621 tagccccgac cgtatggcgg ttggggtggt ctccacgcgg tgcggcgacg cgatagttct
     4681 cccgctccca ctttgagttc caggcgccga ttcgggacgt cgagctcgat ttcgtctccc
     4741 tctctcacga gggcgagcgg gcctccgacg tgtgactcgg gggcgacgtg gagcacgcag
     4801 gtgccgtagg cggtgccgct catgcgggcg tcggagatgc gcaccatgtc ccttactccc
     4861 ctttcgagca gctttttggg gatgggtagc aatccccact ccggcatgcc gggggccccg
     4921 atggggccga ccccccttag aacgatcacg gagtctgcgt ctacctcgag gttcgggtcg
     4981 tctatgcgcc tggcgaggtc gtcgtagccg tcgaagacca cggccggtcc cttatgcctg
     5041 agcatccctg ggtctgcggc agagtgcttt atgacgcagc catcgggggc cagattgccg
     5101 cgcaacacgg cgagtccgcc ctcatactgc agggggtcgt cgagcggtct gatgacctcg
     5161 tctttgtaga cgccggtgag tgtctcgccc atggcgtgac cgccaacggt ggcacggtcg
     5221 aggtggagca tctccgcgag gcgggagagt aagccgcgta ggccgccggc gtaatagaag
     5281 tcctccatca ggtactcgcc gcccgggcgt atgttggcga ggacgggtac gcggcgtgag
     5341 atctcgtcga agcgtgcgag gtccatgggt acgccgctgc ggcgggccag ggcgatgaga
     5401 tggatcacgg cgttggtaga gccgccgagg gccatcacgg ccgtcacggc gtcctcgtag
     5461 gagtggccgt ccaggatgtc cgagggcttg aggtcatccc agaccatctc gacgatcctg
     5521 cgcccgctcg acgaggccat cctggggtga ccagagtcgg cggcgggtat ggaggaggct
     5581 cccgggaggg tcatgccgag gacctcggcg acggcggtca tggtcgaggc tgttcccatg
     5641 gtcatgcagt ggcccggcga gcgggcgata ccgtcttcta tctcttccca cacgcattct
     5701 ccgatggtgc ccgcgcgctt ctcgtcccaa tacttccaca cgtcactgcc gcttcctaga
     5761 gtgctgccgc gccagttgcc gcgcagcatc ggtcctgcgg ggacgaagat ggccggcagg
     5821 ttcatcgtga tcgcacccat aagcagggcc ggtgtggtct tgtcgcaacc gcccatcaac
     5881 accgtgccgt ctatgggata agagcgcaag agctcctctg tctccatggc gaggaagttc
     5941 ctgtagagca tcgtcgaggg tttctggaac ggctcggaga gtgacatggc gggcagctcg
     6001 accgggaacc caccggcctg ccagacaccc cgtttgacct cctcggccct ctggcggaag
     6061 tgggcgtggc aggggtttat gtcactccag gtgttgatga tcccgacgac cggcttcccg
     6121 ctgtagtcct ccgcggcgta gcccatctgt ttggtccgcg agcggtggcc gaaggagcgc
     6181 aggtcgcgca ccccgtacca gcggtggctg cgcagctctt cggcccttct cttccggttc
     6241 atgttctccc ttccaccgat cgtccatgcg cgcaggcggc gacatgattc ccgagacgct
     6301 ccattttcat tctaccctcg gcatcaggta ccgtccttca ggaagcacgt agacacgtag
     6361 tctgaatcga tgctttcaag ggcattcatc tgccgcgaat cgcacattcg atgactagcc
     6421 gttcttccgg gcgaaaacta gccgtacggg tgatgcccgt cttccggagc agggatagag
     6481 tatccgtgta caccaacagg atcaggagag aaccatggag cacacggata ctttggccgc
     6541 cctgtcggac ggcatggcag atgcggtaga gagagtggcg acgtcggttg tgcgggtgaa
     6601 cggacggagg cgtcgttctg ggagcggcgt ggtctttgcg cagaacaagg tgctgacggc
     6661 gagccacgtg ctcgagcgcg aggaagacct ctcggtagag accgtcggcg gaagaaccct
     6721 gacggcgcgg ttcgcgggga gggaccactc cacggacctg gcggtgctgg acgtggaggg
     6781 ccttgacgtc gaccccgcgg cgccagcgga aggagaagcc agggtcggcc agatctcgct
     6841 ggccgtgggc agccactccc gcggcgaagg gccgagggcc accctggggg tggttagcgc
     6901 cgtcggaggc cctgtgcgca ccaggcgggg accgaggcta gaacgttaca tacagaccga
     6961 cgcgacacct taccccggtt tctcgggagg gccactcatc gacgtgcggg ggaacgtact
     7021 gggcatcctg gtctcgggct ggggacgtgg cgcggcgttc gccatacctg ccgacctggc
     7081 ctggcgtacc gcggggacgc tctcggagcg gggctccgtc aagcgcggat acctcggcat
     7141 cctgagccag ccggtgcgcc tccccgacgg acagcggctc ggcctctcgc aaaggggcgg
     7201 attgctggtc gtaggcgtgg aggacggcag tcccgcgggg cgtggcggcc tcatcgtagg
     7261 cgacatcctg gcgacgctgg acggacagcc cgtcgaggac accgacgacc tgctcgtgct
     7321 gctggccgga gacagggtcg gccgtcctgt acccgtcggg gtcgtgcgcg gcggcgagct
     7381 cgcggagatc gagatcaccg tgggcgagcg cggttaggag ttcgggagat ggccgaattg
     7441 tcctcagact cctccgccgt ccggatgata gaggacgtac agaggagcgt cgtacaggta
     7501 cgaagtagcg gccggggtat cggcgccggt gtgatctggc caggcgacgg cctcgtcctg
     7561 acgaaccatc atgtgatctc aggacgacgc aggagaggca acatcagggt ggctctccac
     7621 gacggccgga cgctcgacgc cgatgtagta aagagcggcc ggaggttgga cctcgcgctg
     7681 ctcaggttag agggcgacca cggagacctg ccgcctgccc ccgtcgggga ctctgacgcc
     7741 ctgcgcgtcg gcgaactggt ctacgctatc gggcatccct ggggccgggt gggggccgtg
     7801 agcgccggca tcgttggcgg agtcggcgaa gttcgcgggc aaggccggag ttcctccacg
     7861 cgctacgtac ggtcggacgt tgccctcgcg ccgggaaatt cgggcggacc gctcctgaac
     7921 gcaaggggtg aggtcgtcgc catcaacgct atgatattcg gccttatggc gctctctatc
     7981 ccaagcaacg cggcgcaggc ctgggtcgcc ggagaacgcc gtccgaggct cggtatcggg
     8041 gtcttgcccg tcgaggtgcc cgtatctctg cgccgggaag ccggacctgc cgggctcgtg
     8101 atcgctggcg tcgagacagg tggcgctgcc gacagggccg gcctgctggt cggcgatgtc
     8161 ttgctcgcca tcgccgggaa gccgctcggg gaggcaggga ccttgttgga gaccgtggcg
     8221 cacgcaggtg atgttgtctc cttgcgggtc atgcgcggcg gtaaaatcat ggtcatggaa
     8281 gtgcgtctgg aagagtcggg gcgcgccgca tgacgcatac gcgggtgttc gtcgtggccc
     8341 ccacgccaac catgcgggcg gggcttcgtt cgatgctcgc ggccgtcgag gccgccgact
     8401 tgctggtggt cggcgaggcc ggtctggccg ccgggcccgt tcccccgctc tctggtgccg
     8461 acgtggtgct cgtggctgac gaggagttgc tcgaagagac cgcgctctcc gtatcggaag
     8521 acggtactca gtcgctactg ctgctctcgg aggacgaggg cacggcgtcc aggctgcgca
     8581 ccctgtctct aaggggttgg ggcgtcgtgc cgcctgacgc cccgtccgaa gagctgggtg
     8641 ccgctgtcgc cgcggtggcc cagggactga ccgtgctccc gaaaaccctg gccgagcggt
     8701 tgttcgacga gcctgctgca gcagtcgaag aactctcaga gccactgacc gcgcgtgaga
     8761 gcgaggtgct cggcctgctc gggcgcgggc tgtccaacaa gatgatcgca cgcgagctac
     8821 acatcagcga acataccgtc aagttccaca tctcctcgat ctacgccaag ctgggggtga
     8881 gcaaccgcgc cgaggcggtc agtcagggcg cgcgacacgg tctgatctct ctttgagaga
     8941 gcatttcagc acgctcaggc ttcgccttcg cttgtcagca cttcagcttg tcagcctttg
     9001 ctttcggtga tcatcgggcg ctcggaggcg aacgcgtgga ttgctgagga tcggtagctg
     9061 tgcaagttgg attgagcgaa ccatcgctaa cgcgtatctt ctactcccgg tatagaatgg
     9121 agtcggccat gcggggtggt ggaaggcagg gcaatctcgg gctctcctat gaaagcggcg
     9181 attctggcta tcctggcact cctctttgtt gggtgcgaga gagatcggga taccgggacg
     9241 tcgcattctg cgggcggtaa tagacagcct gaacctgtaa tgtaccagga gcctacacgg
     9301 gttatcgtta ccagcgacga cgcctcgctc cctgacggtt gccacccccg gcaggtggca
     9361 gggctcgtga tctccttcgt caatgctttc aacagcgggg atcaggccag cctgtcccgc
     9421 atcttcttcc tgtccgaggg tcccagtccc cctgatttcg cggatagggg ctatgatccg
     9481 tggtcatggt acaccgtaag cgaagtcgat acggaaggca gaatagagag cggcttcgtt
     9541 acctacgacc agggcgaact gctccggtac ttcgccaaga gacacagaaa gggagagcaa
     9601 ttgaggctgc tcaagatcag cctgacgcgt acgggcctgc tcggcaaaga agaaaacgtc
     9661 ggattcgtct acgtcctcaa ccggaccgcc agggacctcg aaccgaacct cggtggaccg
     9721 gcgcgtatag cctctggcca gggtgctatc aactgtacga accgccgcat cttcgcctgg
     9781 cgcatggata tgagggccga agataggcgt acggcccgcg aagccagcaa ctggctgtgt
     9841 accgacccgc ccggttggaa gcctggcaaa gccgttgtcg cgtgtacttg aggggtcagc
     9901 acgtagagtg ccaccctaga aacctcttat gctagtgctc ctgttccctg gcggccgggt
     9961 aggatcaaat gcaggtctcc gaactcgtgc cagaggtagc ctcttttcag ggcttcggag
    10021 taggcgactt cgagatgctc cgcgccggat gtggcaagtg ggcattccga cacgcttccg
    10081 acgagcgcct ccagcatcga cagatgggtg gagcgcggtt cgtgcagccc ggtcagcatc
    10141 gcgtcaacgg accgtatgcc gcgctccggg gttatgaaga ctccggtcca gccctcgcca
    10201 gggtgtgtcg agccgtcgct atcggtgacc gtctcgaggg cgcgcacgac cgtggtcccg
    10261 actgcgacca ccctgtgtcc cgccgtgcgg gccgcgttga tcgcggccgc ggtctcaggt
    10321 gggacacggt agaactcctc atatggcggc tcgtcttctt ccaggcttgc tacgcccgtg
    10381 tgcaatacga gcggcacgac cctgacccca ttcgctacga gacgagtgat cagctccggg
    10441 gtaaaagccc ttcccgccga aggcatctcg gcgctaccta cctcagtggc gtagaccgtc
    10501 tggtagtaga ccgccggcca gctctccctg acgtagcggt acttgatcgg tgacccgtta
    10561 cgctccagat aattattgaa gttcacaggc aggttcaggg tggagatcca gagccggttg
    10621 ggcacttcgt cgcgctgccg cttctccgat agataagggg tgtgcaggac catgctggca
    10681 cctgcaggga gtgacacgat ctcaccagac tcaccgtcga gcacaggttc atcgccggag
    10741 gatgatctca actcgacgac ccaaaggtcc gccggcaggc gggtcgagag gtgcacggtg
    10801 aggggcgtgc cgcccctgcg ctcggctggg agcgcggctt tcattgtgcc gctggtgttg
    10861 acgacgaggg tatctccggc actgagaaac tcggggaggt cgccgaacgt ggagtggacg
    10921 acgctattat cctccaggta ggagaccatc aggcggactt cgtcgcggcg cagtcctcgt
    10981 gcctcgggtg gctcgccggc ctccagttct ggaggcaact cgaagtggag cggggatctc
    11041 gcctcaggat agtcgggaaa agagtctatg gtttccaaag cttctgtaat agcgttggac
    11101 aatttggccc tctccttcta tgaatcagcg gcggttgtgc ccaccctcgc ggccagctcg
    11161 cgggcctcgt agcgtccgct cggcaggtcg cccgtcagga gctcaatgaa cccgggcacg
    11221 ctctcttccg ggagcggtcg gtcgctgatg tcctcgtcgg ggaacgcttc ctgttgcatc
    11281 tgggtcctca tatcgccggg atcgacccgg tagacgcgca gcccggggtt ctcggccgcg
    11341 agtatattgg agagctgttc cagtgcggcc ttgctcgacc cgtatccgcc ccagccttcg
    11401 tacggctcga cagcggcgtc gctcgtgacg ttgataatgc gcgcctgtgg tttcagttcg
    11461 ttgttgagcg cctggatgag cgcgagtggt gagatcacat tgttacggta gacctgttcg
    11521 atcacgtcga gcgggtagtc gagcaagtcc ggctgggggc tcgggccgag gaagctggcg
    11581 ttgttgacga gcgcgtctac gccgccgacc tcgcgtgccg cctcagcgag atcctctcga
    11641 tgttccgggt cggtaacgtc tccagagatg gcaattacgt gcgtgtatct ggacagttcg
    11701 gcgcgggcgc tctcgagggc ttcctcggtg cgggcgtcga tgatcagggt ccagccgtcc
    11761 tcggccagag tgcgagcgag cgccaggcca aggccgcggg aggcgcccgt gatcatcgcc
    11821 gtgcgatccg atgattctcc gctcatttcg tatgcctcct cattttgttt gaatacaatg
    11881 tatcgctctg tcccacgctg ggaaacgtca ggagggacgg ttttcggcct gtactttggt
    11941 acagtacggc ttctggcaac gggacagtag gacacctacg gtctcatcgc tccgctgttg
    12001 tgaaatgcgc tgaggtgaga tggtttcgcg ggttaggggg cctccacgag cccgcggcgg
    12061 acggcgatcc tggccgcctc ggtacggttc ttggcttcga gcttggcgag gatggagccg
    12121 acgtggaact tgaccgtccg ttccgagata aagagtcgct cggcgatctc ggcgttcttc
    12181 aggccgcggg cgaggagcgc gagaacctcg agttccctgg atgtgaggcg gcggttcgaa
    12241 gggacgttct cctcaccctc tatacgctcc agcagcctgt ctgcgacggc aggttcgagg
    12301 agggaacctc cggcctggac ggtgcggatg gcgccgaaga tctcctttcg ggaagcgccc
    12361 ttgagcaggt aaccgcgggc acccgcacgc aaagagccga gtattcgagc gtccgtgtcg
    12421 taggccgtga agacgacggt acgtgcgcga gagccagcgt cgcggagcct ctccagggcc
    12481 tccacgccgt cggtcccagg catctcaaga tccataagta tcacatcagg gtccaggatc
    12541 gctgccagcc ttacgacttc ttccccgtct gcggcctcgc ccaccacctc gaagtcaggt
    12601 tgggtgccga gcacggcgac gaggccctcg cgcagcatag gatggtcgtc ggcgacgagg
    12661 agcctcacct tatccactct gcagcccttc gagcggaacg gtaacatcga tgcgcgtacc
    12721 ctcgccgggg ccgctctccg tctccagaga gccgccgagc atctcgaccc tctccctcat
    12781 gccgacgatg ccatgccgat catccccgac gctggaaggg tcgaaaccgt ggccgtcgtc
    12841 acttacgatg agttgcactg tctccagtgt ggccactagc ctgaccgtta cgcggcttgc
    12901 gccggcgtgg ctggcgatgt tggtcagagc ttcctggcag acgcggtaga gggcggcctc
    12961 gacccgcggc gggagcgggc gactgccgtt cacagccctg aatctcgtgg ccacgtccgt
    13021 ctctgcttcc cagcggtcga cgagagtctt tagcgcctcg gggagggagc ggccttcgag
    13081 tggggcggcg cggaggtcga gtaccgagcg ccgggcctca tcaaggttgg aacgggtgag
    13141 tgagagggcg cggcgcagcg cttcgcgggc gcgctccgcg ccggactcgt cgtcgagcaa
    13201 ggcttcggcc gactcgagct ggagtcccgt ggcagtaagc ccctgagcga gggtgtcgtg
    13261 gatctcacgc gccaggcggt tgcgctcctc gacggcgccc agcctggcac tgcggtcgaa
    13321 gaggctggcc cgttcgacgg cgatagccaa caggtcgcct atggtggaga ggagctgcag
    13381 gtcttctgga gagaggcctc gccactgggg gctcgcgacg ttcattacgc caagcttctt
    13441 ctccccggcg tagaggggga tactggcgtg gtaacgtaga ccgccggtgc cgtcgaccag
    13501 gccatcaaga cggctgcaag tgaggacgtt gacgttggca gcgccttcca ggtcgcccct
    13561 cttgtaagtg tccaggcaat agcagtagcc tgagccgtcc atgcggctcg ggtcgtccgc
    13621 gagggcagga gggaggttgc gggccgcggc caggtagggc tcgggagaag agtcgtctat
    13681 gagccagatc caggctgtac gcaatcccag tagctcggcc acctgcgaca gcgtgaaccc
    13741 gagggcctcg ccaagctcga cagagcggtt gagctcgcgg gcgatctcgt tgagcaccga
    13801 gagctcgtgg ttgcgccgct ccaggtctct ggtctctcgc acctcagaag catcgggcat
    13861 cgcgtccgta cctcctctcg cgagagttta tcagcgggat acgacaccag ggccggaaac
    13921 tagccgtttg ggtgatggga cgctcggtgc ggagccgccg gaaggttagt ggccccgttt
    13981 gttcctgaac cggcgtcgcc tgaggacgta ggcgagtaac accagcgcga cgagtccgat
    14041 ggcgccgtag ccaagggacc tcacgatggc gagcagctcc tcccaatact ggccgaagac
    14101 gtacccgacc agcgagtagg tcacggccca tacgacgcca gcggccaggt tgtaggggag
    14161 aaacctgtag tacttcatcc tggttacccc ggcgaagagt ggggtcatgc tgcgcagacc
    14221 cggtccgaag cgtccgacga agacggtctt cccaccgtgc gcctcgaagt agcgttccac
    14281 cctggcgagg ctcttctcgt tgaccaggaa atggaggaaa cgcatcttga ggatgcggta
    14341 gatcatgggt ctaccgccga tgcgcccgat ccaatacacg gagttgtcgg agatgaaggc
    14401 ccccgcgaag ccgcttagca tcactagcgg gagcgcgagt cgtccgccgt tggcgaacaa
    14461 cccgcctgcc agcagaacga tatctcccga agcagggaga ccgaagttgt ccagggccgc
    14521 cccgaagagt attaccagat agccgtgggc tacgagcagg ttcgttacga aacccagaac
    14581 accgctggga gagcttggga tggatctgat gaagtccaga aggctttcca tgcgccggag
    14641 gggttatacc acgccgcacg ctcctgcata cgtaacggtt gacgcgtgct ggcagcccgg
    14701 ctatgctgct caatgacaaa cagggggtga aggggtccag gaagccggtg cgagtccggc
    14761 acggtcccgc cactgtaacc ggggagcgaa ccccaagagg ccaccgccgc agcgggaagg
    14821 cagggttagc agagagccgg gagtcaggag actggccttt cgcccgtgac cccaaaccgg
    14881 ggcgaggacc ccgagaagga gacatctatg gagaagatcc tcgggcggag atacccttcg
    14941 accctgccgc tgtggagcct gcttaccctg gctctgttgc tcgttgcgtt gttcgtccta
    15001 ctctcggcca gcggggccct gctcgcacca ctcctggggc aggccgcggg ccccttcgac
    15061 tacctgcacg agttcgccca cgacgggcga cacctgctcg gcgttccctg ccactagggc
    15121 cacccgtggt ctcggcctac ctgcgccgcg gcatggccgc cgggttgctt gccggcctcc
    15181 tcgccggcct cttcgccttc ctcgtcgggg agcccatcct cgatcgggcg attgcactcg
    15241 aggaggcctc ggctggcgct caccatgagg aggtcttctc ccgcccgacc cagaagatcg
    15301 gcctgttttt cgccacggga ctcttcgggg tgactgtagg tggggtgttc ggtatcggct
    15361 acgccttttt tcgcgaacgt ctggccgctg gcagtgattt caagcgcagc atcttcctgg
    15421 ccggtgccat cttcgtgggg gcgttcttga tcccctacct caagtatccg gccaacccgc
    15481 catccgtcgg cgacccctcc accatagggg agcgcacctt ggcctacttc acgctcgtcg
    15541 ccctctcgct cctcgccatc ctccttgcct ggctggccgc aaggacgctc aggtcacgcg
    15601 gtgtcggtgc ctcgcgacgt cgtctgacgg taggcgctgg cctggtcgtc gtcatcggcg
    15661 tgctgttctt gctcttgcct gctgcgccat ccgccgatga gtttccgtca gggacgctct
    15721 gggctttcag gctctcttct ttcggtaccc agctggcctt ttgggccggg ctcggactac
    15781 tcttcggggg gctgtgcgag cgtgccagga ggaagggcgt gaccgcatga acggagccct
    15841 actcgtcgca gggacccact ccgatgccgg aaagagtgtc gtcgtggccg gcatctgccg
    15901 ctggctggcg cgcgagggtg taagcgtcgc gccgttcaag gcccagaaca tggccctcaa
    15961 ctccttcgtg acgcgggagg gcgctgagat gggacgcgcc caggcctccc aggccgccgc
    16021 cgcgatgata gaacccgagg tggccatgaa cccgatcctg ctcaagccct ccgccgaaag
    16081 gaccacgcag gtcgtcgtgc gcggcaaggt acgggcgaac gcgagcgcca caggctacgg
    16141 ggggatgaag aagacgctga tgcccgtggt gctggagtcg cttgaagagt tgcggcggcg
    16201 tttcgacgtt gtcgtctgcg aaggggcggg gagtcctgcg gagatcaacc tgcgggagaa
    16261 cgacctcgcc aacatgggtc tggcgcgtgc cgccgacctg cctgtcctcc tcgttgggga
    16321 catcgaccgc ggcggcctct ttgcgagtct ctacgggacg ctcgcactcc tctcgccgga
    16381 ggaccagtcc ctcgtcgcaa gcttcctcgt caacaagttc aggggagatc cggcggtcct
    16441 ggccccaggc ctggaacgga tgacctcttt gacaggccgc ccgttcctcg ggacgctgcc
    16501 ctggatcccc ggtctcggcc tcgacgggga agactccctg gccctcgacg ccccacgacc
    16561 cgtgaagcct ccgctgggcc gcgacgtgct aagcgtcgcc gtcgcccgcc tcggacggat
    16621 cagcaacttc acggacttcg acgccctggt ccaagagccc ggcgtctccg tgcgctttac
    16681 cgggttggcg gaagagatac tggatgctga cctcgccgtc ctcccaggaa cgaaagcgac
    16741 ggtcgaggac ctgaaactgc tccgctcacg cggaatcgac catgccttcg ccgaaagggt
    16801 gaagcgtgcc ctgcctacgc tgggaatctg cggcggctac cagatgctag gcaggcgcat
    16861 cgaggacggc gtggagagcg gcgaggctga agtccttggg ctcggactcc ttcccgtcga
    16921 gacctccttc gaagtggaga agatgctagg acgtccacaa ggatgggccc ttggtttcgg
    16981 gggcgcggaa gtagcagggt atgagatcca ccacggaaga gtcaggcgag aaggaggcga
    17041 ccctctcttc gataccgtcg aaggcaccga gggctgccgc tcaggagcga cgctcggcac
    17101 gtcctggcac ggcatcctag agtcagacgg cttccgtcgc gccctgttgc tatgggtgac
    17161 cgaagaacgc ggcctcgact ggaagccggg cgaaatgccg ttcgccgcag cccgcgaagc
    17221 gcaactggag aagctcggga atctggtggc ggatcacgta gaccgtgaag ccttgctgag
    17281 attgatagac gtggggcagc cctcgggact gcccgtcatc gggctacagg ctaggggcta
    17341 caagcctcga gaggggaatg gcgacggatt gttgacgggg acggcagagc ctgtgggtct
    17401 gcccccgaac gcgcccgtaa acctgaaagc tgaaacctga tgcctgaaac ctcgagatct
    17461 aagctgcttt tccttaccac cgccgacacc gagatcctgg ccgccgctag agcctcggaa
    17521 cgtctgcccg aaggtttccc gatgctcttg tgcgccaacc ctgttgtcct ggaagacccc
    17581 gcggcgttct tcgagaggga gttgcctggg gccggggcgg tcctcgtgcg gctgctcggc
    17641 ggacggaagg cttggccaga gggactcgag gagttgcggc ggcggtgcgc gcggcttagg
    17701 gttccgctgc tcgccttcgg cggggaggcc gagccggacg cacagctgac cgcactctcg
    17761 accgttcctt cgggcacagt gctggaggct ttcgagtacc tgcgttacgg cggcgtccgt
    17821 aataccgaga acctgctccg cttcgtcgcc gacaccgttc tcatggaagg ctacggcttc
    17881 gagcctgcgt cgccgctgcc agaggtgggt gtctaccatc cggggttgcc cgagggctct
    17941 acgggcgaag aactgctcgc gcggcacgat ccggcgcggc ctacagtagg agttatcttc
    18001 taccgcgcgc actggatgag cgggaacaca gccttcgtgg atgcgctcgt cgaggcgctg
    18061 gaggccgccg gggccgacgc cctgcctgtc tactgctact cgttgcgggc aggtcctgac
    18121 ggacaggtac aaggactgga gctcctgaaa gaaagagtag attgcctcgt caccaccgtc
    18181 ctcgccggtg gtgggtccaa cgcggcggac gccaggagat ccggctctcc ccaggagtgg
    18241 ttggagtggg acgtcccggc actcgaagag ctcggagtcc ccgtcataca gggcatctgc
    18301 acgacctcga cgcgggaggc ctggctcgcc tcggacgccg gcctctcccc gctcgacaca
    18361 gcctggcagg tggccatacc ggagttcgac gggcgcaata tcggggtccc cttctcgttc
    18421 aaggaacgcc tgggcgaaga ctcgcccgtc ggcgccccgc tcaccctcta caaagccgac
    18481 cccgaacgca ccgcccgcct ggcaggactc gcggcgcgct tcgccagact cgggcgtatc
    18541 ccgaactcgg agaagaaagt cgccgtgctg ctctccaact acccgaccaa gcactcgcgg
    18601 gtagggaacg ccgtgggact ggataccccg gtaagcgcca tcaagttgct ggacgcaatg
    18661 cgggaggcgg gctataaggt ggagggtgcg ccagagagag gggacgaact catccactcg
    18721 ctcatcgggg ctgggggcca tgatctggag ttcctgaccg gggaccagct cgggaacgct
    18781 gccggacgcc tggatgcgaa acgctacgcg gagtggtttg gccggctgcc agaggaactg
    18841 cggagcgacg tcgaggagca ctgggggagg ccgccggggg atctgtacgt tgatggcgac
    18901 gagttcgtcg tggctgggct ctctttcgga aacgtcttcg tgggcatcca gccgccgagg
    18961 ggcttcgggg agaacccgat cgccatctac cacgatcccg acctgccgcc gacccaccac
    19021 tacctcgctg cctactggtg gatgatcgag gagttcggcg ctgacgcgat agtccacctc
    19081 ggcaagcatg gtacgctgga gtggctacct ggcaagtctc ttggcctctc gccctcatgc
    19141 gcccccgatg ccgccctgcg cgacgtgccg ctcttctatc cctttgtcgt caacgaccct
    19201 ggcgagggga cgcaggccaa aaggagggcg cacgcgaccg tcgtcgacca tctgatccca
    19261 cccatgaccc gcgctgagac atacgacgat ctggcgaagc tcgagcaact cctggacgag
    19321 tattaccagg tagagacgct cgacccctcg aagctgccag cgataagggt catgatctgg
    19381 gagaccctcc gcaatgcgga gctccaccgc gacctcggtg tcgaggagca gcccgaggag
    19441 ttctccgatt tcctcaccca cgtcgacgga tacctttgtg agatcaaaga cctccccatc
    19501 aggggaggcc tccacatcct cggccagaca cctgatggcg agagcctgcg ccacctcctc
    19561 gcctcgatcc tacgcctcgg cgccggtgaa gtacctggat tgcgtcgcgc cgcaggcgcc
    19621 gccttcggcc tcgacgaaaa ggttcttgcg gagaacggcg gcacgaaggt cgaggctccg
    19681 cccgggctcg tcgagcgctt ccccggtaca gtggtaacgg cctccgatct cctcgacagg
    19741 ctggaagagg cgcagcaggc tctgctcagc gggatggagg agcgtggctg ggacgctggc
    19801 tctgcacgcg cggtgtgcga gaagactctg ggcgtcgccg acgctggcgt agagcggtct
    19861 ttgcgctttg cggccgagga ggtagttccg cgcctgatgc gcacacccga agagatgggg
    19921 aacctgctct ccggtctcgg gggtggttac gtgtctgcgg gaccttccgg ttccccgacg
    19981 cgcggcctga tcaacgtgct cccgacaggg cgcaacttct actcggtgga cccgaaggcg
    20041 ctgccgagcg cgctctcgtg ggaagtcgga caggggctcg cggacgacct gctgcggcgc
    20101 tatctcgaag aagagggccg ttatccggag acggtcggca tcgtggtgtg ggggacggcg
    20161 gccatgagaa cccagggcga cgacgtctcg gagatcctgg ctctgctcgg cgtgcgaccc
    20221 gtctggaacg aggagtcgcg gcgggtcgtc gggctggagg taattccacc aagagagctc
    20281 agacgaccaa gagtagacgc aacggtcaga atcagcggct tcttcaggga cgcttttccg
    20341 aatttgatct ccctcctcga cgaagctttc accaccgtcg ccgaactcga ggagcccgat
    20401 cacatgaact tcgtcaaaaa gcacgcgggc gaggagagga gccggggcgc cgacgaacgc
    20461 cgctcgacca cgcgcatttt cggatccaag ccaggcgcct acggcgctgg acttcttccg
    20521 cttatggacg cccgcaactg gcgtaccgat gaggacctcg ccgaggtcta cgccgtctgg
    20581 ggagggtatg cctatggtcg tgggttagac ggcgtcgagg cgcgggaggc catggagtcc
    20641 aacctgcgca ggacggaggt cgctgtaaag aacgtagaca accgcgagca cgacctcttc
    20701 gactcggacg actactttca ataccacgga ggtatgatcg ccgccgtccg cgcccttacg
    20761 ggcagcgacc cgaaagcgta catcggagac tccgcagacc catcgagagt gaagacgagg
    20821 gatctctccg aggaggcccg ccgcgtcttc cggagccgcg tcgccaaccc gaagtggata
    20881 agggccatgc agcgccacgg ttacaagggg gccttcgagc tctcggccac cgtggactac
    20941 ctcttcggct acgacgccac agcaggcgtc gtcgaagact ggatgtacag cgacgtaacc
    21001 aggaaatacg tcctcgacca ggaggcaagg gacttcatgc agcagtccaa cccctgggcg
    21061 ctgcgcgcca tctcggagcg cctgctcgag gcggcccaac gcggcctgtg gtccgaaccg
    21121 gacaccgaag accttgaagc tctgaaacag gtttatctgg agaacgaggg gatgctagag
    21181 gaaggtatct catgaacgcg tacctgcatc cctatccatt ctcggccatc gtgggccagg
    21241 acgatctgaa actggccctg ctcgtgaacg ccgtctcccc tgaggtaggg ggcgtgctcg
    21301 tacgaggcga gaaggggacg gcgaagtcca cggcagtacg ggcgctggcc agcctactgc
    21361 ccagcatacg ggtcatctcc ggctgcccat tttcctgcga tccggaagcc cccaacccgg
    21421 aatgcccggc gggaccacac ccgcttgatg cctccgccga gacgaggccg gtgcggctcg
    21481 tggagctacc tgtgggggcg agcacggacc gtctcgccgg gactctggac atcgagaagg
    21541 cgctctcgga gggcagaaag gcgttcgagc ctgggttgct ggcggcggcg cacaggggca
    21601 tcttgtacgt ggacgaggtg aacctactct ccgaccacct ggtggatttg ctcctcgacg
    21661 tagccgctat gggcgtaaac cacgtcgagc gcgagggcgt gagcgtgcgg catccctcgc
    21721 gctttatgct ggtgggcact atgaaccccg aggagggcga gctccgcccg caactcctcg
    21781 accgcttcgg catcaccgtc gaggtcgcag ggagcccaga cccagctgac cgggtcgagg
    21841 tcgtcaggcg cagactacgc tacgagactg cccctcagga gttctccgga gaatggtcga
    21901 gcgtggacgc caagcttgcg gcttccgtgg aatcggcccg tctgcgcctt cccggcgtca
    21961 gcctggacga ggacatcttg ttcaggatct caaccctctg cgcggaactc ggcgtcgacg
    22021 gcctgcgtgg cgacctcgta acggcgaaga ccgcgcgtac gctggcggcc tgggacgcca
    22081 ggaacgaagt ggtcctcgac gacgtgaagc gggcagccct gctggccctc tcgcaccgcc
    22141 gcagacgcgg ccccttcgag cagcccggca tggacccgga ggacctggag aacgtcctct
    22201 ccgaagacag ccccgacccg ccggacgacc ctggcggcgg ttcgataagg cctgccacga
    22261 gtgagaatga ccgcgaggtt tctagtagcg tgcgggatcc cgaaccctcg gaggcacaca
    22321 agggagccgg agagcggagc cacgcagctt cggagccttt cgaccctgtg cggctcgagg
    22381 ttaccgagaa gggcaaggga gggcctctcg ggcgcaggag tcgggtctcg ggtgagaccg
    22441 ggcatcctgt cgcggatagg gagaacggag ggagaccgag ggacgtggcg ttggcggcga
    22501 cggtaagggc tgcggcgccg caccagaggg cgcggggagg agagggatct gggatcgagg
    22561 ttcgtacctg cgatctgcgt cagaacgtcc gagaggggag ggaggggaac ctgatcctct
    22621 tcctcgtcga cgctagcggc tcgatggcgg cccgcaaacg catgtctgcc gtcaagggcg
    22681 ccgtcctctc tctcttgaac gacgcctacc agcgccgcga taaggtgacc ttaatctctt
    22741 tccgtggcaa aggagccagg attcttctgc ccccgacgtc gagcgtcggg ctggctacct
    22801 cgcgcctgga agaccttccg acaggaggcc gcacgcccct ggcggccggt atcgagaagg
    22861 ccgcggaggt gctgaagagg gaggaactgc gcgacaggga gcgccggcct ctgctggtcc
    22921 tgctgaccga cggaagatcg acggcgggac cggatccgca ggtggccgct gcgcgcatgc
    22981 gctccctcgg ggcaacttcg ttcgttgtgg acaccgagga aggctacgtc aggctcggga
    23041 tggcgggcga gctggcaggg gcgatggggg cgcggtgcct gcgcctcgag gaattacgag
    23101 ccgattcgct cgtggagctc atcgagagga gaagggtggc gtgagcgaga cgagggaacc
    23161 gagagagcag cggctgagaa ggaggtcgaa cagggagagg cctctactca tcgtcaacac
    23221 aggccacggc aaaggcaagt ccacggcctc cttcggcgtc atgctcaggg gctgggcccg
    23281 cggctaccgc ataggggtct accagttcgt caagagcggc aaatggaacg tcggcgaaca
    23341 gaaggccgcc gaggctctgg gcaacatcga ctggttcaaa cagggcgacg gatggacgtg
    23401 gaccgtaaag gacctggagc actccgccga cctggcccgc gaggggtggg aggaggtcaa
    23461 acgccgtctc gcagacgaga cctacgacat gctgctcctc gacgagttca cctaccccat
    23521 gaagttcggc tgggtggata ccagcgaggt cgttgaggtt ctaaagaacc gtcccggctt
    23581 ccagcacgtc atcatcacag gccgcgacgc cccgcctgaa ctggtggaga tctccgacct
    23641 ggtgagcgag gtaaataagg tcaaacaccc cttcgacgaa ggctatcggg gccagaaagg
    23701 tatcgagtgg tagacacccc ccgcatagtg gtcgccggta cccactcagg ggtcggcaag
    23761 acgactattg cttcgggact catggcggcg ctctcgttgg agggctttcg ggtcgccccc
    23821 ttcaaggtcg gccccgactt tatagacctc tcgtaccaca ccctcgccgc aggcaggcag
    23881 ggacgcaacc tcgacgcttt cctctccggc cccgacctca tcggcccgct cttcgcccac
    23941 ggcgcacaga gtgccgacat agccgtgatc gaaggcgtta tgggcctctt cgacggcaaa
    24001 ggaggcgggg atctcgcctc cacagcccac gtcgccagac tgctccaggc gcccatcgtg
    24061 ctcgtcgtcg acgcgagagc aatggcccgc tccgcggccg ccatggtcca tggatatacg
    24121 actttcgacc caaaggcgag ggttgcaggc gtcgtcctca accgcgtcgg ctccagggtc
    24181 cacgagtcga tgctccgcga agccctgaga cctctcggaa tccccgtcct cggcgtcctg
    24241 cgccgcgacg acgatataca gacgccagac aggcacctcg gcctcgtccc cgtcgccgaa
    24301 cgccgtgagg aggccactcg ttcgctccat gccctgggaa aagtgacctc gcgttctcta
    24361 aacctcgatg gcattgttag acttgcccgt tccgcaggac ctctagtagt cgagccctgg
    24421 agtcctgagt ctccggacct ggataggtcc tgtggcgttc gcgtggcggt tgcggcggga
    24481 ccttcgttca gcttcctata ccgcgagaac ctggagctgc tggagggcgc gggcgctgag
    24541 gtcgtcacct tcgacccgac caccgaggag gacctgcccg ctggaacaga cgctctctac
    24601 ctcggtggtg ggttccccga gacctacgca aaggtcctct ccacgaacgg gcggatgcgg
    24661 gagagtgttc gggcctttgc agcaggcggg cgcccagtgg ttgccgagtg cggcgggttg
    24721 ttgtacctgg tgcgcgagct tgacgggcat cgcatgtgcg gcgtgctcga cgcagaggcc
    24781 acgatgacgg accggctcgc gctcggctac agggaagccc gcgcgctggc ggactcgccg
    24841 ttggccgagc ggggcgccaa cgttcgcgga cacgagttcc actacgctgc ggtcgagacg
    24901 cgcggcgaat cgaggccggc ctgggacctg atcggccgcg gtaaggaggg ctttgtggcc
    24961 ggtggcgtgc acgcgagctt cctgcacacc cactgggcag cggtgcccga gttgccgcgc
    25021 aggctggtgc tggaggcgtc gctgagcgcg acggaggtcg gagtatgagc gggcggctcg
    25081 tcggggtggg ggtagggccc ggcgaccccg aactgttgac cgtcaaaggt ctgggcttgc
    25141 tgagggaggc ggacgaggtc ttcgtgcccg tggctgacac aggcgaggta gggcgggccg
    25201 aggccacggt gcgcgagtat ctggacgaaa ggcgcataag acgcctcccc ttcgcactct
    25261 cggacgacgc ggaggccagg gaacgtaact ggaccaacgc ggcgcacaga gtctccgagc
    25321 acctgcggag cggcaagacg tgcgcgttcg ctactgtcgg agaccccaac gtctattcga
    25381 ccttcaccta cctggcacgt gccgttcagg agatcgaacc tgacgttgag gtagagactg
    25441 tgccagggat cacggcgttg caggacctgg cttcgcggag tgggacggta ctcctcgaag
    25501 gcgacgagag gcttgcgctg ttgcctttca cggcagggga ggagcctctg agggaggcgc
    25561 tagccgggtt cgagacggtg gtctgctaca agggtgggag ccggctcggg gaggtgctcc
    25621 gggtcgctga agagggggga cggctggagg gcgcggtgta cggggcacgg ctcgggatcg
    25681 agggcgaaga ggtggtgcct gcgctcgaga tggtggggcg agagggaact tacctgtcca
    25741 ccgtgatctt tagcgggagg agttcggagt ttgggtaagg tgtggtttat cggggctggg
    25801 cctggggcgc ctgacctatt gacggtcagg ggggccaggc tcatcggtga ggctgacgtg
    25861 gtggtctggg ccaggagcct cgttcaggag ggcgtcctcc aatatacccg acctggggcc
    25921 cagatagtcg agtcgacgac cataccgctc gaagacgtgc gcgggctcta cgagcgggcg
    25981 gtggatgagg atttgagagt ggccagggtg cattcgggcg accccagtct gtacggtgct
    26041 gtgatggagc agatcgagct ctgcaacgag attggcctcg attgggagat agtgccaggc
    26101 gtctcgtctc taggcgccgc cgcggcggcc atcgggcgcg agctgaccgt gcccgaggtc
    26161 tcacagtctg tgatcctgac tcgccgtgcg agccgcaccc cgatgccaga cggcgaggac
    26221 ataaggagct tcgcggagca cgggacgact atggcgatct tcctctccgc cgcgaagccg
    26281 cgcgtcctgc agcaggatct tctggaaggc ggttatgccc ccgagacccc ttgcgccgtt
    26341 gtctacaggg cgagctggcc cgacgaagaa gtcatcgagt gccgcctcga agacctcgcc
    26401 gacagggtgc gggaggccgg cttcaccagg caggcgctca tactggtcgg cccagggctc
    26461 ggtgcccaag ggacgcgctc tcatctctac agccccgcgt tctcgcacat gttccgccgg
    26521 gcgaagcctg cggagaaggg agcagatgcc gccacgtaag ctccgcgagc cttccatgcc
    26581 cccgagcatg gagaaggtca agggccagaa actcaggaca ggctggacca caggcacctg
    26641 cgccgctgcc gcagccaagg ccgcggccag cgcgctggcg agcggccggc cacaaactcg
    26701 cgtcgacgtg aagctgccag ggaaaggcag ggagcgacgc gtggagttcg atgtagagcg
    26761 ttgtgatctc ggtggttcct gggccgaggc cgtcgtcatc aaagatgccg gcgatgaccc
    26821 cgacgtcaca gacggcgccc atctcacagc ccgcgtctcg tggctggaac ggcaggggct
    26881 cgagctggac cggggtgatg gcgtcggcag cgtcaccaaa cctgggctcg ggctccctgt
    26941 cgggggacct gccataaacg ccgtgccgcg gcgaatgatc tcctactccg tagaggaagt
    27001 cctcgacccg gacgagcgcg gcgtccgcgt cgtcataagc gtccccggcg gcgagaagat
    27061 ggccgagaag acgacgaacg cccgcctcgg catcgtcggc ggcatctcca tcctcggtac
    27121 cacgggcatc gtgcgcccct tctctacggc ggcctgggcc gcgagcgtgg ttcaggccat
    27181 agacgtcatg ggcgcgcagg gtcacaagac cttcgtcctc tcgaccggag gcctcaccga
    27241 gaaggcggcg atgcggctcc tgcccgacct cgaagaggtg aacttcgtag aagtcgggga
    27301 ctttaccggg caggccataa agaaggccgt ggagaacgga ctggagcgct gcttcttcgt
    27361 cgggatggcg ggtaagatct ccaaactcgc cgccggggtc atgatgaccc actggacccg
    27421 ctcgaaagtg gataacgatc ttctggcaaa gataaccctc gaggccggcg gctcgaccaa
    27481 cctggtagaa gaggtcaaag gcgccaatac ggcccgtcac gcatacgagc tctggcggtc
    27541 ggtgcacctt gatagaggag ctgacctcct ctgccaacag gccgcggaga atctcgccga
    27601 gtacgctgaa ggtaagctag aggttcacgt catcctcgtc gacttcgaca ccctcgaacc
    27661 tgcgggagcc agtctcggcg ccctgacgct gacgacgtgg ggccccacgt gagccggggc
    27721 cgcataaccg tcgttggtct cgatggaaga cctctcgacg aggagatcgc acgtctgctt
    27781 gcggacgcgg cgctcgtcgc cggcggcagg cggcacctgg agatgctcgg tgttgagagc
    27841 gaccgtgcct tggtcttgga gggagacctc tctgaggcgc tcgcacggat cgaagctacc
    27901 ggtggtcccg tggtcgtgct cgcatccggg gaccccggtt tcttcggcat cgtgcgcctg
    27961 ctcgccgggc gctttgggag ggaaaacctg cgtatctggc caggactctc ctctgtcgcg
    28021 ctggcgttcg cccgcgccgg gctctcctgg gacgatgcgg tcacggtcag cgcccacggc
    28081 cgggatcccc gtcgcgccgt caacgtctgc cgcgcccacc ccaaggccgc cgtcctcacc
    28141 tccccggact tcgggccagc cgagctcgcg gcagcgctcg acggtctggg ccgcagcttc
    28201 gtggtcgcgg agaagctcgg tgggccccac gagcggatct tcaacggcga cgcaggcacg
    28261 gtcgccggca tggtgtggga agacccaaac gtggtactcg tgctcgacga agagaggata
    28321 ccgggcgcca gaggttggat ctcctccagt ctccagagtc cgctgcggtg ggcgcttccc
    28381 gaaggagagt tcgagcaccg gtctggcatg atcacgaagt cagcgacccg cgccttcgta
    28441 ctctcacgcc tcggtccggg gccgggagac ctgatctggg acataggcgc cggcagcggc
    28501 tccgtagcca tcgagtgcgc ccgtctgggc gccgccgcca tcgccgtcga aagcgacccc
    28561 gaaagctgcg cccgtatccg ccgcaacgcc gaacatcacg gtgtccggtt gcagatcatg
    28621 gaagccacgg caccgcaagt cctgtgggat ctgtccgaac ccgacgccgt cttcgttggc
    28681 gggagtggcg gagccttcga ggagatcgtc aagctctccg ccgttagggc gcgccgggcg
    28741 gtcgtgttgg ccctcgtcac cctcgaacgg gtcgtccctg ccgcacagct cctcgaagat
    28801 tgcggcctcg aggcagagac caccttcctg caagcgtccg gaatgaaggg cgtcggtaat
    28861 ctgcaccgcc tcgctgccga gagcgccatt ttcgtcgtct cggggagccg cacttgatcg
    28921 ggctcatagc agccaccgct aacggccgcc gcaacgccgc gcatctcgcc ggcacctggc
    28981 ctgatacgcg cctctacgag ggcaaggcga aagtagctct caagcgggcc tggtacgagt
    29041 gcgacggcat cgtgctcttc ctcgccaccg gggccgctct ccgcctcctc gcgccgctgc
    29101 tcgcaagcaa acaacaggat cccggcgtgg tcaccgttga cgacgccggc aacttcgttg
    29161 tcgccctctg cggcgggcac gaaggcgggg ccaacgccct cgcggcacgg gtcgcggata
    29221 ccctgggcgg gacgccggtg atcaccaccg caagcgactc tgttggtttc cccgccctcg
    29281 actctctagg cgataggcta ggtctccggt tggagggtga ctcggatctg gcttccgtcg
    29341 gggccgcgct cgtctccggt gagaaggtca acttcgtcac agacaggcgt tggccgctcg
    29401 gacccctacc ggagaacgtc ggaaggaaag acgaatggga ggcgccgctg atcctcatct
    29461 ccgataggct cctggagacg ccccgtcctg ctgtggtctt ccggccgcct tcgctcgtcg
    29521 ctggcgtggg ttgcagcagg ggagtggacg cggaagagat actcgaactg ctccttcgct
    29581 ccctggcgca agctgaattg accgagaaga gcgtcgcggc cctcgccagc atcgacgtga
    29641 agcgcgacga ggctggaatg ctgcaagccg cggagaagct ggacgtgccg atccacttcc
    29701 atccggccga agcgctctct gtggtcgagg cgcccaaccc gtcggagctg gtgcgagacg
    29761 cggtcgggac gccgagcgtc gcggaggccg cggtggtgtc ctccggcgcg gagttggtac
    29821 tcgagaagca gaaatcggcg aacgccacgg tcgctatcgg gcggctgccc gtgcggggga
    29881 ggctcgcgct ggtcagcctg ggacctggag aggatacgct tattccaccg ctagcccgcg
    29941 aggcactcgt ctcttctgag tttgtcgtgg gactggacca gtacgttgac cgcatcaggc
    30001 atctgctgcg acctgggacg cgggtgctca cgcctccgct cggcaatgag gtggagcggg
    30061 cggagctcgc gctagccgag gcccgcgcgg gcggcagcgt ggcgctggtg tctagtggtg
    30121 acgtgggtat ctatgcgatg gcgtctccgg ggctcgagct cgccgacgag gacgttgacg
    30181 tcgtggtagt acctggggta acagcggcgc aggcggcggc ttcgctgctc ggatcgccgc
    30241 tcgggcacga tcactgttcg gtgagcctct ccgacctgct gacgccctgg ggggtgatcc
    30301 aatccagggt cagggctgcg gccgagggag acttcgtggt ctcgctctac aacccacgct
    30361 cgaagggccg cgactggcag ctcggaaagg taaaggagat cctgctcgag caccgtccac
    30421 ctgatacgcc ggtcggcata gtaaaggacg cctaccgcca gacacagcgg gtcatcctca
    30481 ccgacctcgc ttctctgcgt caggaagatg tggacatgct gaccatcgtc ctcgtcggaa
    30541 gctcccagac cagggtagtc gccggccgca tggttacccc acggggttac ctatcatgaa
    30601 ggcagtcttc gccgtgacag aagcctgcgc cggttgcgga gcgtgcataa agacctgccc
    30661 ggagaaggcg atccttcctg ctcccaggga ctatggtgcg cccctcctcg tcctcgaaga
    30721 ccgctgttcg ggatgcgccg aatgcgccga gatctgtccg gtctcggcgt gcatcgaggt
    30781 attgctggag gccgaatgag gcacgtccat ccaatagaga cggagagcta ccgcatcctg
    30841 cgcgggatgg tcgatctctc gtacctggga cccctctctc gcgccgtcgc ggaacgggtc
    30901 atccatgcct cggcggatct ggactacgca gagaacctcg tcctcgacga gacagccctg
    30961 gaaaggggaa tctcagcgct gcggcgagac ggacccgtcg ttacagacgt ggggatggtg
    31021 gcggccggga tcacaggccg ccagacgctg tgcttgatct ccgacccccg cgccaaagac
    31081 ctatccgaac agcttggcat aacgcgttcg gcggcgggct tcagggtcgc tgcggaggag
    31141 gtcggcaaag gagccgtctg gatcgtcggg aatgcgccta cggcgctctt cgagctgctc
    31201 gggctcgacg ttgatcccga tctcgtgatc ggtcttcccg tcggcttcgt cggggccgcc
    31261 gagtcgaagg aggagctcgt agccagcggt ctgcctgccg tcgcgaacag gggaccgaaa
    31321 ggcggttctg cggtcgcagc agcggctgtg aacgctctca tctattacga ggagaaataa
    31381 tatgaggcga cctgtaggta tcgaaataag ggaaacctac ggagacgccc cggcgctgct
    31441 gatcgtaggc cacggcagcc gcgactcacg cggggcagcc gagttccacg acctggtcga
    31501 cctggtgcgc aggcgaaacc catccctgac ggtcgaggga ggctttatcg agctctcgcg
    31561 cccaccaatc tcggagtgcg tggaccggct cgccgacagg ggggcaagga acgccgccgc
    31621 cgtcccgctg atgctcctgg ccgcaggtca cgccaaggac gacatcccag cgacactggt
    31681 gcgcgagaag atgggccacc ccgaaatgag cttcaactac ggtcgcgccc tcggtataag
    31741 gcccgagctt ctcgaactga tggacgagag gatctccacc gtcgtcctcg aggaggagaa
    31801 gggtgagaca gcggttctcg tcgtggggcg cggctcctcc gatcccgacg ccaactcgga
    31861 cctctccaag atagcccgcc tgttctacga gggacgccct taccccgtag tcgagagtgc
    31921 ttacgtcagc atgacgcagc ccgacgttgc ggaagggctg gatcgctgcc tcaaactcgg
    31981 cgccaggcac gtcgtggtct tctcctactt cctctttacc ggcgtcctcg agcaacgtat
    32041 ccgcgggcag ggcgaagcat tcgccctggc gaaccccggc gtcgagttgc gctacgcagg
    32101 gtacttcggg cctgacgagc tggtggctga ccttgtggtc gagcgttata cagaggccgt
    32161 ggaaggggac atccgcatga actgcgacgt gtgcgtccac cgcgtcgccc tgcctggctt
    32221 cgaggagaag gtcggcgccc cggcaacgcc ccactaccac cctgatgaac ccgggcgcca
    32281 ccagcatggc caccatcact agccgtggcc ctcgaagacc gcctactgga gctcgctggg
    32341 gagatactac cgcccgacga gaccgcagcc gaagaagcgc gaaaccgaca cctcgggctt
    32401 accaaaccgc cctctagcct gggagtgctg gaggaactcg gcgcgcggct cgctgggatg
    32461 gcaggtgatt gcccgccgcc cgtacctgag agccctgccg tagttgtctg tgccggcgac
    32521 cacggcgtac tcgaccgcgg tgtctcgccc tggccgcggg aggttacggc ggcgatggtg
    32581 agtaacttct gcgcaggagg ggcggcggta aacgccatcg cgaagacggt gggggcgaga
    32641 gtgagcgttc tcgacgtcgg ggtcgccggg gagttggaga gacaccctct gctgcgagcc
    32701 gcgaaggtgc gacgcgggac aggcgacctg agtcgcgggc ctgcgatgag ccgcgaggag
    32761 gcagcgcggg ctgtgatggc gggggcggga gtcgcggagg aactcgtcga gagcggtggg
    32821 gtggatctcc tggttacggg cgacatgggc atcgggaaca ccacgccggc tgcttgcctc
    32881 atctccgctt tcgcgggaag cccgcccgag gagaccacgg gccggggcac gggcatcgac
    32941 gatcagactc tgaagctcaa ggtcaacgtg gtagaagagg cactggagtt gcactcgcca
    33001 gaccccgaag acccacttgg ggttcttgcg cagatcggtg gtctggagca cgctgccatc
    33061 gtcggagtga tcctgaccgg cgccatctac ggcatccccg tcatactcga cggcgtagtc
    33121 tcggactccg ccgcgctcgt ggcctgcgcc atcgctccgc actcggcggg atacgtaatc
    33181 gcgggccacc gctctgccga gccaggggct cgaatcgtcc tggagtctct gaaactggaa
    33241 ccactcctcg acctggacat gcgtctcggc gaagggacag gaggcctgct cgccgtgccg
    33301 ctcgtgcaag cagcggcccg gatgctcgcg gagatggcga cgctcgagga gttgggcatt
    33361 ggttgagcct ctagagcggg gatacatcga ggccttgaga agtccgcttg cccgggtcgt
    33421 cgaggttcat ggggcgttgg gctcgaccca ggagcgcgcc cgcgagcttg cccatgctgg
    33481 aacacctcac ggaacgctca tcgtcgcgga gatccagacc gggggacgag gccggctcgg
    33541 acgcccctgg ggatctccga tgggtgggtt atggatctca cttgtgctgc gtcccgaatt
    33601 cgctgcgagt ctcgcttctc gcatcaccca gaccgcgtct gtaggagttg cgaaggcatt
    33661 gtgggaggtt ggggtagaag cacgcatcaa gtggcccaac gacctgctcg ctcacggaaa
    33721 gaagatctgc ggtatcctcg ccgagtcgag cgcggggaac gtcacaggac cggcaagtga
    33781 gaggtacctc gactacgtca tcctcggggt ggggatgaat gccaatttcg acccggccga
    33841 gctcgcggtc cctgaccgcg aggtcaccac gatccgttac gagttgggcc gtgacgtgaa
    33901 cctactagac ctgctccgcg ctctactttc gaacctcgag accgagctcg gcaggatcga
    33961 ggacttcggc gccgtactcg aggattggcg taacgtgaac tgtacccttg gggagagggt
    34021 gcgtattctc cgtctgggcg agaccatcga aggcagggct gtggacctga ctgcagaggg
    34081 cgctctattg ctcgttaccc gacggggcac cgtggaggtg ttcgaaggtg agatcgaaca
    34141 cctcagtcaa ggagtcgagg taaggagcca gctcgaccca ggtcctactt gagtgggagg
    34201 catcaggcca caggtatctt gagacgtgcc aatcaggcgc gcttttgatc cggctgccca
    34261 aagaggagga gaagctgatc gagaagttcg gagacgagta ctgaagctac atgaagcgca
    34321 ccgggcggct cttaccacgc atagagcgag attagagcgg gagccgtggt tcccggtccg
    34381 gctgattatg tatcggttga aacatcaagg ggttacacat cgctcgctct taccaaatct
    34441 gcttctgtga gaaatcctgt ggcccgagac ctgtgccttt tctgtgcgga gggtcagttc
    34501 tttcgcacag cgcttcaggc agtaggcatc tcctcgtctt cttcatcctc ttccaggacc
    34561 gcaaccgagg tcgctctgaa gatcaggatc ttacccctct cggggaagtc cacgatctcc
    34621 acgtccccgg ttacggctat ctggtcccct atgagctcat cagcatcctg tacggccgac
    34681 atgtctatct ccgtctccag aacatacccc gtatgctcct ctccggcttc cgtactcgcg
    34741 cgcttgagct gtccttcgaa gtaggccacg gccgatcccc tttccgattg tcagtgggga
    34801 catagtaccc ttattcgcga acgaggaccg gggcaccgtt ctctgtgccg gcacggccag
    34861 agcctatgac cacagatcgc gcgtctggaa gggttcgggc ctccgacacg taaaccggga
    34921 atgcgcccag acgcgattct agctgcggga cgcgaaaccc tacaagaagg gcgcttcagg
    34981 cagggccggt tgcgatttca tggctcgtag gatttaatac tctcctgggc aggagcctcg
    35041 accctggatc gggcatcgtt gggaggcaga taagagggat gaacgcaacg ttgatgtatg
    35101 tcggcatcgg ggccgtactt gctacaggcg tggcgattct cactatagct gtcctattct
    35161 tgcagaccgc acgccgctac gtcgacctga ccgagagacg cctggagctc ctgcgagaag
    35221 gggaagcgtt cctgctcaac ctcgtgcagc ggcagggcca ggctttggag cgggaagcgg
    35281 agcgacgcga gaaggccccc gagatggtcg gaaatcctcg tggacggcag cagggcgaag
    35341 aatccactgg cagccggcgg tctgaagggt tgaaccagcg cgtccaggaa acgccgtata
    35401 cgggcagctc ctctgaagta gaagatgaag agcagaggcg ggagcggaca ccgaagccgg
    35461 acccagcgcc ggcgcggccc aaagacaatg cgccgcggct tggagttcag gtgcctcacc
    35521 ctgacgacga tgtgcccgga cgcggctgga acaactcacc ggccaagttc ttccagaagt
    35581 gctacgacag ataccttgag cattacgagg gctacgtaag gctcgccgag cggcttcacc
    35641 agatgcgcga cgaggccgat gcggctcctg gcagccttgg ggagtgggag gacaagctcc
    35701 gccgggcata cgacgccata gagcggacta c
//
LOCUS       DASTKM010000328        10171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1027438, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000328 DASTKM010000000
VERSION     DASTKM010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027438"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1473)
                     /gene="purF"
                     /locus_tag="VFQ10_13050"
     CDS             complement(13..1473)
                     /gene="purF"
                     /locus_tag="VFQ10_13050"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET9966316.1"
                     /translation="MGSLDVDYRDTDGKPKEACGVFGLYSRELSGELAQRTYFGLYAL
                     QHRGQESAGIAVSDGERTTAMRDMGLVSQVFDGPRLAALEAGHLALGHVRYSTTGSAS
                     WENAQPEFGGRGDVNVAVAHNGNLVNAGKLRDDLAEDGFRFYSTSDTTYIAAAAVREL
                     ERGLSVGEAVRGAMRRFEGAYSVAMIFRDKLVAFRDPHGFRPLCIGEVEGGYAVSSET
                     CGLDIIGARFLRDVEPGEVVVIDDEGLTSYRVETPRRALCVFEYVYFARPDSRFDGRE
                     VAGSRHRMGELLAEEAPAEADVVIPVPDSGMMAAIGYSQRSGIPYAEGLIKNRYVGRT
                     FIQPTDGMRQLGIRLKLNPLPSVIEGNRVVVVDDSIVRGNTSRKLVELLFEAGAAEVH
                     FRVSSPPVKGPCYYGIDMDSPDQLVGANLTVEEIRAQIGATTLAYLSTDAMVAATQQR
                     KDRLCRACFDGDYPTTGTASKFALENRTQYAASKGG"
     gene            complement(1497..3767)
                     /gene="purL"
                     /locus_tag="VFQ10_13055"
     CDS             complement(1497..3767)
                     /gene="purL"
                     /locus_tag="VFQ10_13055"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET9966317.1"
                     /translation="MEDTAFRAGGGRGLNIQETYTSLGLSDFEYDRILELMGRAPNFL
                     ELSLFSVMWSEHCGYKNSRPLLGRFPNEGTHVLQGPGENAGVVEVGDGWALAFKMESH
                     NHPSAVEPYEGAATGVGGIIRDILAMGARPVALLDSLRFGPLEEERNRYLFREVVRGI
                     GGYGNAVGVPTVGGEVEFGRAYSGNPLVNAMCIGLIRVEELVRSSATGIGNLVVLLGS
                     KTGRDGIHGATFASDELTDGSESKRSSVQVGDPFCEKMLIECCLKLLDEMLLVSLQDL
                     GAAGITSSASEMAAKGGVGIEIETDRVPLRESGMEPTEVMISESQERMLAVVEPEKVD
                     EVLRIAERYELVGAVVGRVAEHGDLRVIHDGEVVGTVPAEHLADAPVYERDVVRPRYL
                     DEVPELDLDEIPEPEDYNAVLLQMLGHPNLRSRRPIFTQYDHQVGTDTVVLPGADAAV
                     MRIKGTDLGFAAATDGRGRHCYLDPRGGGAATVAEAYRNLSCVGAEPVAVTDCLNFGS
                     PEKPDGYFQLAECIEGMSEACEVFGTPVVSGNVSLYNETDRGAIYPTPTVGMVGVLED
                     VDRHASPGFQREGDLVVVIGDGSPVSLEGSEYLEIVHARVSGRPPQPDLGAGKAIADL
                     VREMVRSGLVDTAHDISGGGEIVAVAEMALAGGLGVVYEEGELQRMTAGRGGGRADVA
                     LFGEGSGAFVVAVPEERRDELQDALVGFAYDVVGTTRGDRLEIGDLIDIELSDLEEAY
                     ERDLFEVHAPEGGLIG"
     gene            complement(3725..4387)
                     /gene="purQ"
                     /locus_tag="VFQ10_13060"
     CDS             complement(3725..4387)
                     /gene="purQ"
                     /locus_tag="VFQ10_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET9966318.1"
                     /translation="MRIGVTVFPGSNCDRDALYGVERMGAEAVELWHADADLKGVDAV
                     IVPGGFSYGDYLRPGAIARFANVMGPLEEFAKSGGPVLGVCNGFQVLTEAHLLPGALL
                     RNTHMRFVCDRVRVRVESVDTPWTAGCDPGEELTLPVAHNEGNYFADPATLAELEEEG
                     RVVLRYLENPNGSANDIAGVCNEDRNVVGIMPHPERVSDPALGSDEGLRILLSVLVGA
                     GV"
     gene            complement(4384..4608)
                     /gene="purS"
                     /locus_tag="VFQ10_13065"
     CDS             complement(4384..4608)
                     /gene="purS"
                     /locus_tag="VFQ10_13065"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HET9966319.1"
                     /translation="MKIRVLVRPKAGILDPQGEAVKNALPALGFAGVRTVHVGRLVEM
                     EVEGSDEVDAMCRRLLSNPTIEEYEWEVVG"
     gene            complement(4605..5330)
                     /gene="purC"
                     /locus_tag="VFQ10_13070"
     CDS             complement(4605..5330)
                     /gene="purC"
                     /locus_tag="VFQ10_13070"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET9966320.1"
                     /translation="MTEELLYEGKAKKVFTTDDKDVLLHRYKDEATAFNAQKRGSWKD
                     KGKTNATMSAAIFAYLETHGVPTHFVEQVDDTTIKTKKLDMLPVEIVVRNVAAGSLSR
                     LLGFEEGRKLKAPLVELCYKDDDLGDPLLNWYHFRELGVSDEDLEFCEELGLKVNEVL
                     APFFDERGIILVDFKIEAGRDAEGNLMLADEISPDTCRFWDKETGEKLDKDRFRNDLG
                     GVEEAYAEMLRRVTEITQRGAVD"
     gene            complement(5323..6636)
                     /gene="purB"
                     /locus_tag="VFQ10_13075"
     CDS             complement(5323..6636)
                     /gene="purB"
                     /locus_tag="VFQ10_13075"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET9966321.1"
                     /translation="MIERYTLPEIGAVWTEEAKYRAWLKVELAVSRARAQLGEIPAEE
                     VEELAEKADFTVGRIQEIEQETNHDVIAFVTNVAEYAGDVARHFHFGLTSSDILDTAG
                     ALQLREALDLILDEARTLTLLLAKMALEHRDTVMVGRTHGVHAEPTVLGHKLAVWAFE
                     MERNLDRLSHAREVAAVGKISGAVGTYANVDPKVEAITCQELGLSSSPASTQVVQRDR
                     HAEVLSTLAILGSTVEKIALEIRGAQRTEVRELAEPFGRGQKGSSAMPHKRNPILAER
                     LCGMARLLRANATVGFENNALWQERDISHSSAERVVLPDSTILAFYMLRTTIRILRGL
                     EVHKDRMLENLNSGGGIVFSGRVLLALVESGMNRDDAYRVVQDAAMRAWQGGDGFREL
                     LSMDDDVGERLGDELDTLFDPAYALRNLGAVFGRVDELNRRLEND"
     gene            complement(6677..7108)
                     /gene="purE"
                     /locus_tag="VFQ10_13080"
     CDS             complement(6677..7108)
                     /gene="purE"
                     /locus_tag="VFQ10_13080"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET9966322.1"
                     /translation="MSPRVGILVGSESDLPILEKCTARLDGYGIEYELEVRSAHRNPE
                     GVSEYAGTASERGLKVIICAAGMAAHLAGAVAARTNLPVIGIPVASGTLNGFDALLST
                     VQMPSGVPVATVAVNGAANAGVLAAQILALSDPELAEKLER"
     gene            complement(7105..8379)
                     /gene="purD"
                     /locus_tag="VFQ10_13085"
     CDS             complement(7105..8379)
                     /gene="purD"
                     /locus_tag="VFQ10_13085"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET9966323.1"
                     /translation="MKVLVVGSGGREHALVETLAASPLEPEMYAAPGNVGISRIAETV
                     DIPVDDLVSLRDYAANNEIDLTVVGPETPLIGGISEAFWEAGLKVFGPSRAAARIEGS
                     KVFAKELMRHAGVPTARFEAFDREAAALAYLRTHHDYPIVVKADGAAAGKGVTVAHTQ
                     DDAEEAVRASFAGKFGAAGERIVIEECLEGREASVFVVTDGHEILPFLPAQDYKRVND
                     GDEGPNTGGMGSYSPIMWMEPTTYATILEDIIRPTIHQLALIGAPYTGLLYAGVMVTE
                     TGPKALEFNCRFGDPETQVILPRLGSDLLELMLAAGNEDLAGREIVWSADKAVCVVLA
                     SEGYPESSSLGDEISGLENIPAGVYVYHAATEEREGRFYTAGGRVLNVVGTGPTIIEA
                     RARAYAAVEQIHFEGMHYRTDIALEAIELEDL"
     gene            complement(8392..9678)
                     /locus_tag="VFQ10_13090"
     CDS             complement(8392..9678)
                     /locus_tag="VFQ10_13090"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET9966324.1"
                     /translation="MTATVVLGLAWGDEGKGRVCDALASDVDYVSRYSGGNNAGHTVR
                     VGEEEFKLHLVPSGIVREGVVCTIGNGVVVDPEVLVEEVEALEERGLGVRDRLKVDGR
                     AHLIMPYHIALDSHREIALGDARIGTTNRGIGPAYEDKVARYGIRVQDIFDEGILKTK
                     LKAALREKNAIFENVYGEEHYEVADVASWLLSFREFIGPMVADTGTLLREALDNRERV
                     LLEGGQATLLDNDHGTYPFVTSSNPTVGGAIVGSGISARHLEYVVGVTKAYTTRVGDG
                     PMPTELFDETGETIRNVGREYGTTTGRSRRVGWLDLPAVKWAASLNGITHVAITLLDV
                     LSAVDEINICVGYEIDGQRVEGYPMHQTDLHHARPVYKSLPCWGEDITWCRMRGDLPR
                     AAQDFVGFVEAEVGAPVCMISVGPERDQAIVERIGI"
     gene            complement(9968..>10171)
                     /locus_tag="VFQ10_13095"
     CDS             complement(9968..>10171)
                     /locus_tag="VFQ10_13095"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9966325.1"
                     /translation="IVNEDLPDDEPVAYAFINNLRLNQDELISLENEITAAGDNPAKG
                     VQSWLEENRDVVQPAIDAAKQAQ"
BASE COUNT         1763 a         3430 c         3055 g         1923 t
ORIGIN      
        1 cggatgtgca attcatccgc ccttgctggc agcgtattgg gtccggttct ccagggcgaa
       61 cttgctcgcc gtgccagtcg tcgggtagtc gccgtcgaag caggcgcggc agaggcggtc
      121 cttcctctgt tgcgtggctg ccaccatcgc atcagtagag aggtaggcga gggtggtggc
      181 cccgatctgg gcccggatct cctccaccgt caggttcgct cctaccagtt ggtcagggct
      241 atccatgtcg ataccgtagt agcaggggcc cttgaccggg ggcgaagaga ccctgaagtg
      301 gacctcggct gcacctgctt cgaagagcag ctcgaccagc ttgcggctgg tgttgccacg
      361 tacgatggag tcgtctacga cgaccaccct gttgccttcg atcacgctcg gcagcgggtt
      421 gagcttgagc cttatgccga gctggcgcat accgtcggtc ggctggatga aagtcctccc
      481 gacgtagcgg ttctttatga ggccttccgc gtaagggatg ccgctccgct gcgagtagcc
      541 gatcgccgcc atcatccccg aatcggggac ggggatgacc acgtcggcct cagccggcgc
      601 ctcctcggcg agtaactcac ccatcctgtg cctggagccg gcgacctcgc gtccgtcgaa
      661 gcgcgagtcc ggacgggcga agtacacgta ctcgaagacg cacagtgcac ggcgcggggt
      721 ctcgaccctg taactcgtca ggccctcgtc gtcgatgacc acgacctcgc cgggctccac
      781 gtcgcgcagg aacctggcgc cgatgatgtc caggccgcag gtctcgctgg agaccgcgta
      841 gccaccctcg acctcgccga tgcagagggg tcggaagccg tgagggtcgc ggaaagcgac
      901 gagcttatca cggaagatca tggccacaga gtaagctcct tcgaatcgac gcatcgcccc
      961 cctgaccgcc tccccgaccg agagcccccg ctccagctcc ctcacggccg cagccgcgat
     1021 gtaggtcgtg tccgaggtag agtagaacct gaagccgtcc tcggccaggt catcccgcaa
     1081 cttccctgcg tttacgaggt tgccgttgtg ggccacggcc acgtttacgt cgccgcgccc
     1141 gccgaactcc ggctgggcat tctcccagga agcggagccg gtcgtcgagt agcggacgtg
     1201 gccgagggcc aggtgtccgg cctcgagagc tgcgaggcgt ggtccgtcga agacttgcga
     1261 gacgaggccc atgtcgcgca tcgcggtcgt gcgctcaccg tcggagacgg ctatgccggc
     1321 cgactcctgg cccctgtgct ggagggcgta gagcccgaag tacgtgcgct gggccagctc
     1381 ccccgaaagc tctcgggagt agagcccgaa gacgccgcaa gcctccttcg gcttgccgtc
     1441 cgtatccctg tagtctacgt caagcgagcc caccgtctgg gcctcccttc tctatgctat
     1501 ccaatgagtc cgccctccgg cgcatgcacc tcgaagaggt cgcgctcgta agcctcttcg
     1561 agatccgaca gctctatgtc tataagatcg cctatctcca acctgtctcc gcgggtcgtc
     1621 ccgacgacat cgtaagcgaa cccgaccaga gcatcctgca gttcgtcccg gcgttcttct
     1681 ggaaccgcga cgacgaacgc gccgctccct tctccgaaca aggcaacatc cgctcttcct
     1741 ccaccccggc cggctgtcat acgttggagc tcgccctcct cgtacaccac gccaaggccg
     1801 ccggccagcg ccatttcagc gaccgcaacg atctccccgc cgccggatat gtcgtgcgcc
     1861 gtgtcgacga gcccggaccg gaccatttcg cgcaccaggt ctgcgatcgc ctttccggcg
     1921 ccgagatcag gctgcggtgg cctacccgag acacgagcgt ggactatctc caggtactca
     1981 gagccctcga gagagaccgg agatccgtcc ccgatcacca ccaccagatc cccctcgcgc
     2041 tggaagcctg gcgaagcgtg tcgatctacg tcctccaaga caccgaccat cccgacggtg
     2101 ggggtcgggt agatggcgcc ccgatcggtt tcgttgtaga ggctgacgtt gccgcttacg
     2161 accggcgtcc cgaagacctc gcatgcctcg gacatcccct ctatacactc ggcgagctgg
     2221 aagtagccgt caggcttctc ggggctgccg aagttgaggc agtcggtaac ggcgacaggc
     2281 tccgccccta cacacgagag gttccggtag gcttcggcga ccgttgcggc cccgccgccc
     2341 ctcgggtcga gatagcagtg gcggcctctg ccgtccgtag cagcggcgaa gccgagatcc
     2401 gtgcccttta tccgcatcac cgccgcatca gcgccgggca agaccacggt atccgtgcct
     2461 acctggtggt cgtactgggt gaagatggga cggcgcgagc ggaggttcgg atgccccagc
     2521 atctgcaaga gcacggcgtt gtaatcttcc ggctcgggaa tctcgtccag gtcgagttca
     2581 ggtacttcgt ccaggtatcg cggtcgcacc acgtcgcgct cgtagacggg agcgtcggcc
     2641 aggtgttcgg cggggacggt gccgactacc tccccgtcgt ggatcacgcg caggtcgccg
     2701 tgctcggcga cgcggcctac caccgccccg acgagctcgt agcgttcggc gatcctcaga
     2761 acctcatcga ccttttcggg ctccacaacg gcgagcatcc gctcctggga ctcggagatc
     2821 atgacctccg taggctccat cccgctctcg cgcaggggca ccctgtcagt ctcgatctcg
     2881 atgccgaccc cgcccttcgc ggccatctcg gaggcggaag atgtgatgcc ggcggcgccg
     2941 aggtcctgca aagagaccag cagcatctca tcaagcagct tcaggcaaca ttcgataagc
     3001 atcttctcgc agaaggggtc gccgacctgc acgctggatc tcttcgactc ggaaccgtcg
     3061 gtgagctcgt ccgaggcgaa ggtggccccg tgtataccgt cccgcccggt cttcgaccct
     3121 agcaatacga cgaggttgcc tataccggta gcgctcgaac ggacgagctc ctcgacccgg
     3181 atcaggccta tgcacatcgc gttgaccagc gggttacctg agtatgccct cccgaactcg
     3241 acctccccgc cgaccgtcgg caccccgacg gcgttcccgt accctccgat accacgcacg
     3301 acctcccgaa agaggtaacg gttgcgctcc tcctccaggg gcccgaaccg cagcgagtcg
     3361 agcagagcaa ctggccgcgc gcccatggcc aggatgtcgc ggatgatccc acctacaccc
     3421 gtcgcggccc cctcgtaagg ctccacggct gaaggatggt tgtggctctc catcttgaac
     3481 gcgagcgccc acccatctcc cacctcgacc acgccggcgt tctcccctgg cccctgcaac
     3541 acatgcgtcc cctcgttcgg gaagcggccg aggagcgggc gcgagttctt gtagccgcag
     3601 tgttcgctcc acattacgga gaacagcgag agctccagga agttgggtgc ccgtcccatc
     3661 agctccagga tacggtcgta ctcgaagtcc gagagcccca aggaggtgta tgtctcctgg
     3721 atattcagac ccctgccccc accagcacgg aaagcagtat cctcaatccc tcgtcggagc
     3781 cgagggcggg gtcggagacc ctctcgggat gcggcatgat gccgacgacg ttgcggtcct
     3841 cgttgcagac gcccgcgatg tcgttcgccg acccgttcgg gttctcgagg tatcgcagca
     3901 cgacacgccc ttcttcctcc agctccgcga gcgtcgccgg atcggcgaag tagtttccct
     3961 cgttgtgggc aaccggcagg gtaagctcct cgccgggatc acagcccgca gtccagggcg
     4021 tgtctacgga ttcgacgcgg acacggacac ggtcgcagac aaagcgcatg tgcgtattgc
     4081 ggagcaaagc acctggcaga aggtgggcct cggtcaggac ctggaaaccg ttacagacac
     4141 cgagtacagg gcctccgctc ttcgcgaact cttccagtgg ccccatgacg ttcgcaaagc
     4201 gggcgattgc gccgggtctg aggtagtcgc cgtaggagaa gccgccggga acgatcacgg
     4261 cgtcgacacc cttgaggtcc gcgtccgcgt gccacaactc gaccgcctcg gcgcccatcc
     4321 gctccacacc gtagagcgcg tccctgtcgc agttggaccc ggggaagacc gtaacgccaa
     4381 tcctcacccc acgacctccc actcgtactc ttctatagtc gggttagaga ggagcctgcg
     4441 gcacatagcg tcgacctcgt ccgaaccctc gacctccatc tcgacgagcc tcccgacgtg
     4501 aacggttctg acccctgcga aaccaagagc gggaagagcg ttcttcaccg cctcgccctg
     4561 cgggtcgagg ataccggcct tcgggcggac gaggacccgg attttcaatc tacggcacct
     4621 cgctgtgtga tctccgtgac ccttcgaagc atctcggcgt aggcctcctc gacccctccc
     4681 aggtcgttcc ggaaacggtc cttgtccagc ttctcgcccg tctctttgtc ccagaaacgg
     4741 caggtgtcgg gggagatctc atccgcaagc atgaggttgc cctcggcatc ccggccggcc
     4801 tcaatcttga agtcgacgag gattatgccg cgctcgtcga agaagggcgc gaggacctcg
     4861 ttgaccttga ggcccagctc ctcacagaac tccaggtcct cgtcggagac gccgagttcc
     4921 ctgaagtggt accagttcag caaggggtct ccgagatcgt cgtccttgta gcacagctcc
     4981 actaacgggg ccttgagctt cctcccctcc tcgaagccga gcagccgcga gagtgaaccc
     5041 gctgcgacgt tgcggaccac tatctctacc ggcagcatgt cgagcttctt ggtcttgata
     5101 gtcgtgtcgt cgacctgttc tacgaagtgg gttgggacgc catgtgtctc gaggtaggcg
     5161 aaaatagcag cgctcatggt tgcgttggtc ttgcccttgt ccttccagga gccgcgcttc
     5221 tgggcgttga acgccgtcgc ttcgtccttg tagcggtgca gcagcacatc cttatcatcc
     5281 gtcgtaaaga ccttcttggc ctttccttcg tagagtaatt cttcagtcat tctccagcct
     5341 cctattcagc tcatccaccc gtccaaagac cgcgccaagg ttgcgcagcg cgtatgcggg
     5401 gtcgaagagc gtatccagtt catcgcccaa ccgttcgcca acgtcgtcgt ccatgctcag
     5461 caactccctg aagccgtccc caccctgcca ggcccgcatc gcggcatcct ggaccacacg
     5521 gtatgcgtca tcacggttca tgccgctctc cacgagcgcg agcaggacgc ggcccgagaa
     5581 gacgatacct cctccgctgt tcaggttctc gagcatccgg tctttgtgca cttcgaggcc
     5641 acgcaatatg cgaatcgtgg tgcgtagcat gtagaaggcc aggatcgtgg agtcggggag
     5701 tacgacgcgt tcagccgagg agtgggagat gtcccgctct tgccagaggg cgttgttctc
     5761 gaagccgacc gtagcgttcg cgcgcaacag cctggccatg ccgcacaggc gctcggccag
     5821 gatcgggttc ctcttgtggg gcatcgccga ggaacccttc tgcccgcgtc cgaacggctc
     5881 agccagctcg cggacctcag tacgctgggc gcccctgatc tccaacgcta tcttctccac
     5941 cgtggaaccg aggatggcga gtgtggagag aacttcagcg tgcctgtcac gctgcacgac
     6001 ctgggtcgag gccggtgacg aggaaagacc gagctcctgg caggtgatcg cctcgacctt
     6061 ggggtcgacg ttcgcgtagg tacctacggc gccggagatc ttacccacag cagcgacctc
     6121 ccgcgcatgg gagagtcgat ccaggttcct ctccatttcg aaagcccaca cggcgagctt
     6181 gtgtcccagc accgtcggct cggcgtgaac cccgtgcgtg cgcccgacca tcaccgtgtc
     6241 gcgatgttcc agcgccatct tggccagaag caacgtcaag gttctcgctt cgtcgaggat
     6301 gagatccagg gcctccctta gctgcagggc tcccgccgtg tcgaggatat cagaactcgt
     6361 caggccgaag tggaagtgac gggcgacgtc cccggcgtat tcggcgacgt tggtcacgaa
     6421 cgctattacg tcgtgattgg tctcttgctc tatctcctgg atgcgcccga ccgtgaagtc
     6481 cgccttctct gccagttcct ccacttcttc cgccggaatc tccccaagct gcgccctcgc
     6541 ccggctgacc gcaagctcca ccttcagcca ggcgcggtac ttcgcctctt cggtccagac
     6601 cgcaccgatc tccggcagtg tgtagcgttc gatcactcga tactgacccg ctctctttgc
     6661 ggcgtctcga tcaagcctac ctctctaact tttcggcgag ctcgggatcg gagagcgcga
     6721 ggatctgggc tgcgagcacg ccggcgttgg cggcgccatt gacggccacc gtggcaaccg
     6781 gaacgcccga gggcatctgg acggtggaga ggagggcgtc gaagccattc agggtgcctg
     6841 aggcaacggg tatcccgata acgggcaggt tggtgcgtgc tgccacggcc ccggccaggt
     6901 gagcggccat gccggcggcg cagattatca ccttcaggcc gcgctcgctg gccgtgcccg
     6961 cgtactcgga gacgccttcc gggttacggt gggccgagcg aacctcgagt tcgtactcga
     7021 taccgtagcc atcgagccgg gccgtgcact tttcgaggat ggggagatcc gattcgcttc
     7081 cgaccaggat tcctactctc gggctcaaag gtcctccagc tctatcgcct ccagggctat
     7141 atccgtacgg tagtgcatgc cttcgaagtg gatctgctcg accgcggcgt aggcgcgggc
     7201 ccgcgcttcg ataatggtcg gcccggttcc gacgacgttc aagaccctgc cgccggcggt
     7261 gtagaagcga ccttcgcgct cttcagtggc cgcgtggtag acgtagaccc cggcggggat
     7321 gttctccaga ccggagatct catcccccaa cgacgatgac tcggggtagc cttccgaggc
     7381 cagaaccaca cagaccgcct tgtccgcgga ccagacgatc tcgcgcccgg cgaggtcctc
     7441 gttgcccgcc gccagcatca gttcgaggag gtcggaaccc aacctcggca gtattacctg
     7501 cgtctccgga tctccgaagc ggcagttgaa ctcgagcgcc ttcgggcctg tctccgtgac
     7561 catcacgccc gcgtagagga ggcccgtgta aggagccccg atcagggcaa gctggtggat
     7621 agtggggcgt atgatgtctt ccagtattgt cgcgtacgtt gtcggctcca tccacatgat
     7681 tggagagtag gatcccatcc cccctgtgtt aggcccttcg tcaccatcgt tgacgcgctt
     7741 gtagtcctgg gccggtagga aaggcaggat ctcgtgccca tcagtaacta cgaagaccga
     7801 ggcctctctc ccttcgaggc actcctcgat aacgatcctc tcccctgcgg caccgaactt
     7861 gccggcgaag gaggctctca cggcctcctc ggcatcgtcc tgtgtatggg ccacggttac
     7921 gcccttccca gccgcggccc cgtcggcctt cacgacgatg gggtaatcgt ggtgcgtgcg
     7981 caggtaagct agtgccgccg cttcgcggtc gaacgcctcg aagcgggccg ttggtacgcc
     8041 agcgtggcgc atgagctcct tggcgaagac cttggagccc tctatgcggg ccgcggcccg
     8101 cgaaggaccg aagaccttca ggccggcttc ccagaacgcc tcggagatgc cccctatcaa
     8161 gggggtttcg ggaccgacga cggtcaggtc gatctcattg ttggcagcgt agtctctaag
     8221 ggagaccagg tcgtctacag gtatgtctac cgtctcggcg atgcgtgaga tgccgacgtt
     8281 gccgggggcc gcgtacatct cgggctcaag ggggctggca gccagggtct cgaccagcgc
     8341 gtgctcgcgc ccgccgctcc ctaccacaag caccttcatg gcctcacgac cctagatccc
     8401 gatcctctcg acgatcgcct gatcccgctc cggtccgacg ctgatcatgc acacaggagc
     8461 cccgacttcg gcctccacaa aaccgacgaa gtcctgcgcc gccctgggaa ggtcccccct
     8521 catcctgcac caggtgatgt cctcgcccca gcaggggagc gacttgtaga cgggcctggc
     8581 gtggtgcagg tcggtctggt gcatcgggta gccttccaca cgttggccgt cgatctcgta
     8641 gccgacgcag atgtttatct catcgacagc ggagagcacg tcgagtagcg tgatggcgac
     8701 gtgggtgatg ccgttcagcg acgccgccca cttgacggct ggcagatcga gccaccccac
     8761 cctcctgctc cggcccgtgg tcgtaccgta ctcgcgcccc acgttcctta tggtctcgcc
     8821 cgtctcgtcg aagagctccg tcggcatcgg cccgtcaccg acccgcgtcg tgtatgcctt
     8881 ggtgacaccg acgacgtact ctagatggcg cgcggagatg ccggaaccga cgatcgcacc
     8941 tccgaccgtg gggttggatg atgtgacgaa gggataggtc ccgtgatcgt tgtccaggag
     9001 cgtcgcctgt cccccttcca gcaggacacg ctccctgttg tccagcgcct cacgcaatag
     9061 cgtgccggtg tcagccacca tcggcccgat aaactccctg aacgaaagca gccaggaggc
     9121 gacgtcggcg acctcgtagt gctcctcgcc gtagacgttc tcgaagatcg cgttcttctc
     9181 cctgagggcc gccttgagct tggtcttcaa gatgccttcg tcgaagatat cctgaacacg
     9241 aataccgtag cgagcgacct tgtcctcgta ggcagggcct atgccgcggt tcgtcgtccc
     9301 tatcctggcg tcgccgagcg cgatctcgcg gtgcgagtcg agcgcgatgt ggtagggcat
     9361 gatcagatgc gcccgcccat caactttgag acggtccctt accccaaggc cccgctcctc
     9421 gagggcctcg acctcctcga caagcacctc ggggtcgacc acgaccccgt tgcctatggt
     9481 gcagaccacg ccctcgcgca cgatgccaga aggcacgagg tggagcttga actcctcctc
     9541 gccgacacgg acggtgtgac cagcattgtt gccgcccgag taacgcgaga cgtagtcaac
     9601 atcagacgcg agggcgtcgc acaccctgcc cttgccctca tctccccagg ccagcccgag
     9661 gacgaccgta gccgtcaatg ctctacctcc gcagtagtgt gaaaaggtac gaaacgggcg
     9721 cgacgttcag gaaaaaggag taacctcgat cctggaccga aactcatcgt ccgccgatcg
     9781 gggccgccgc gctctgccac taccgcagcg tagcacatag atttaatgtt ttgtatcgcc
     9841 gggaagaaat tcgtccgtta tgtgcgcagg ataggcgaat gctctggtca aacagcgatt
     9901 cgcggccgga gcgcacgttc gcgctccggc cgcgaagatc ccgcggcttt aggccgggga
     9961 ttcgaggtta ctgcgcttgc ttggccgcgt cgatcgcggg ttgcaccacg tcgcgattct
    10021 cctcgagcca cgactggacg cccttggccg ggttatcgcc agccgcggtg atttcgttct
    10081 ccagtgagat gagctcgtcc tggttgagcc tgagattgtt gataaacgcg taggccaccg
    10141 gctcatcgtc gggaagatct tcattgacta t
//
LOCUS       DASTKM010000329         7147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1203478, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000329 DASTKM010000000
VERSION     DASTKM010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203478"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFQ10_13100"
     CDS             <1..696
                     /locus_tag="VFQ10_13100"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HET9966326.1"
                     /translation="HSVGNIIIAALADMAGGFCEGVEQAARFLRIKGRVLPAATESLT
                     LVVRYADGTVTRGESVVHEVGKQVSEVSVEPAGIPAPPGVIESIEAADMLVLAPGSLF
                     TSTIPALLGGGVREALADFAGPILYVANVMTQQGETGDFTVSDHVRAITEHVGPVVTD
                     VLVHSGDLPPDTLARYEAEEAAPVAVDTEVLREMGLRVRETYLLSKESRAGVRHDPSL
                     LAREVGEAALVRL"
     gene            632..1537
                     /gene="whiA"
                     /locus_tag="VFQ10_13105"
     CDS             632..1537
                     /gene="whiA"
                     /locus_tag="VFQ10_13105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET9966327.1"
                     /translation="MSGTTPHCWRGRWARLPSFASEVRRQLAASAASGPRGVGRSELA
                     GLVDASGVWDEEGVTLRTPSAAVARSVVRLWRSEFGMESRLSSINPDRFGRTRYAVRT
                     AGNGAIQAKEELRFARTARTAAERAGYLRGAFQGAGSVNRPGGRGGHHLEFVHRERDF
                     IRRCVDFSRTPLKIVRRRGRWVAYTKSADGVTTILAQMRLNDAVLEYEARAVLGEAKA
                     NANRVTNFDAANAGRTATAAARQQKAFRAIDPEHLSPALREMLELRLEYPDASLADLA
                     HLGNLSKSATNHRLRRLVSMSARSG"
     gene            1644..2645
                     /gene="gap"
                     /locus_tag="VFQ10_13110"
     CDS             1644..2645
                     /gene="gap"
                     /locus_tag="VFQ10_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET9966328.1"
                     /translation="MAVRVGVNGFGRIGMLFTKAAMDRGDIEVVAANDLMPFDSLANL
                     FKHDSTHGIWHEDVSYDGNVLHVGDHEIKTFAERDPAQIPWGDVGADIVVESTGVFTS
                     RDGAAAHIEGGAKKVIISAPGKGVDATFVYKVNHEDYDPQAHEIVSNASCTTNCIIPM
                     VKVIQDNFGIESGYMTTVHAYTNDQSLLDAAHKDPRRARSAPNNIVPTSTGAARTAGV
                     IYPELQGKVDGMSMRVPVPDGSITDFVARISSEASADDVNAAFRSAADGELSDILEYA
                     EAPLVSSDIIGNKASCVFDSQLTMSNGGTVKVLGWYDNEWGYSNRTCDLAAYMGDKL"
     gene            2803..3975
                     /locus_tag="VFQ10_13115"
     CDS             2803..3975
                     /locus_tag="VFQ10_13115"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET9966329.1"
                     /translation="MNKRSVRDLDVRDKRVLVRVDFNVPVKDGEVTDDTRIRRALPTI
                     RYLLEEGARPILISHLGRPKGEPDPKYAMDPVAARLQELLGEPVKKLDAAVGPEVEEA
                     LEDWDGRGIVLLENSRFYPGETSNDPEFADQLAALADLYVNDAFGAAHRAHATTVGVA
                     ERLPAAAGLLMEEEIDYLDKVLKDPERPFGAILGGAKVSDKLGVIESLLGTADSLLIG
                     GAMCFTFFKAQGYEIGNSLVEDDYLEEAKRLMEEAGDRLVLPVDVVVAEAMEEDAESE
                     TVPVDGIPAGKMGLDIGPKTVDLFSEHISGASTIFWNGPMGVFEFDAFAKGTEGVARA
                     VAESGATSVVGGGDSVAAVNKLGLEDRMSHISTGGGASLEYVEGKELPGIAVLPDK"
     gene            3991..4776
                     /gene="tpiA"
                     /locus_tag="VFQ10_13120"
     CDS             3991..4776
                     /gene="tpiA"
                     /locus_tag="VFQ10_13120"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET9966330.1"
                     /translation="MARRPMMAANWKMNKTVREAQEYTAALLPRAADAEEEVDVAVCA
                     PFTCLREVDRMAGDSAVIAGAQNFYYEDWGAFTGEVSAPMLLDVGAGAVIVGHSERRE
                     VFGETDEMVARKTRRAVEADILPIVCVGETKEERDAGGMWEKVSGQVGRVVEELEDAG
                     GESVVFAYEPIWAIGTGDTATPEDAQDAIGRIRDLLRESKGDDFSGSVRILYGGSVKP
                     ENAAEIMAQEDIDGALVGGASLEVESFVELIGAAQNAVARTEQ"
     gene            4798..5025
                     /gene="secG"
                     /locus_tag="VFQ10_13125"
     CDS             4798..5025
                     /gene="secG"
                     /locus_tag="VFQ10_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET9966331.1"
                     /translation="MLYYIVAFFQVVFCIALVGLILLHSGKGGGLSSSLGGGMGGTFS
                     GTTIMEKNLTRLTLIVVGLFLLTNITLFFMG"
     gene            complement(5412..5499)
                     /locus_tag="VFQ10_13130"
     tRNA            complement(5412..5499)
                     /locus_tag="VFQ10_13130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5463..5465),aa:Leu,seq:gag)
     gene            5580..6470
                     /locus_tag="VFQ10_13135"
     CDS             5580..6470
                     /locus_tag="VFQ10_13135"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET9966332.1"
                     /translation="MRRRHLILISLAGLASFVALLLRKGEKRTVRGGWPVNLAHRGAS
                     ALAPENTIEAFRLAVQDGAGGLELDVHMTRDGHIVVIHDATVDRTTNGSGAVSEMTFD
                     ELRRFDAGHDFSTDGGPTRPYRGRGVQVPTLGEVLEEFPGVAVNIDIKAGTPRIEETL
                     FRVLREADALRRVLVVSIPHARVKRFRKVSGGHVSTGASRREIGIFFISSRLSLERLV
                     RPAYDAVQVPLRHRGIRVVTPRFVRAAHARGVRVDAWTINQADEMRRLLDFGVDVIMT
                     DRPGTLAEVLKNRPTTLTPP"
     gene            complement(6457..6756)
                     /locus_tag="VFQ10_13140"
     CDS             complement(6457..6756)
                     /locus_tag="VFQ10_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966333.1"
                     /translation="MSYVTIQVIWLLCIFVTPPALKLYPSKLWALPFCLVTVANGLHM
                     ILFRYEYNAVLRRAMRILPYAQYLTPLREEPRYFLPLGTAYTLFGVASIVLLMAA"
     gene            6915..>7147
                     /locus_tag="VFQ10_13145"
     CDS             6915..>7147
                     /locus_tag="VFQ10_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET9966334.1"
                     /translation="MISESLVKDPSLAPEGHRKIDWVAQHAPVLNKIAGEQLADGSLR
                     GRKVAMTIHLEAKTAYLALLLSEAGADVTVSGS"
BASE COUNT         1410 a         2136 c         2351 g         1250 t
ORIGIN      
        1 cacagcgtgg gcaacatcat tatcgcagcg ctcgctgaca tggccggcgg gttctgtgag
       61 ggtgttgagc aggcggcccg attcttgagg atcaaaggcc gagtcctccc agcggcgacg
      121 gagagcctga cgctcgtcgt ccgctacgcc gatgggaccg taacgcgcgg cgagtctgtg
      181 gtccacgagg tcggaaagca ggtctctgag gtctctgtcg agccggcagg gattcctgcg
      241 cctcccgggg tcatcgagtc gatagaagcc gccgacatgc tcgtgcttgc tcctgggagc
      301 ctgttcacca gcaccatccc ggctctgctc ggcggcgggg tgagggaggc cctggcggat
      361 ttcgccggtc ccattctcta cgtcgcgaac gtgatgacac agcagggaga gacgggcgac
      421 tttacggtct cggaccacgt gcgggcgata acggagcacg tcgggcccgt ggttaccgac
      481 gtgctcgtac attctggaga cctgcctcct gataccctcg cccgctatga ggccgaggag
      541 gccgcgccgg tcgctgtcga cacggaagtc ctgcgcgaga tgggtttgag ggtgagggag
      601 acgtacctgc tctccaagga gagccgcgcg ggtgtcaggc acgacccctc actgttggcg
      661 agggaggtgg gcgaggctgc cctcgttcgc ctctgaggtg cgtcgccagc tcgccgccag
      721 cgcggcttcg ggcccccgag gggttggtcg ctccgagctc gccggtcttg tcgacgcctc
      781 cggcgtctgg gacgaggaag gcgttacgct acgcacccca agcgctgccg tcgcccgctc
      841 cgtggtgcgc ctgtggcgct cggagttcgg gatggagtcc aggctctcgt cgatcaaccc
      901 tgaccgcttc ggccgtaccc gttacgcggt gcgcacggcg ggcaacggag ctatacaggc
      961 caaggaagag ctgaggttcg cccgcaccgc tcgtaccgcg gcggagcggg ccggttacct
     1021 gcggggcgcg ttccagggcg ccggctctgt gaaccggcct ggcggtcgtg gaggccacca
     1081 cctggagttc gtgcatcgcg agagagattt cattcgccgg tgcgtagact tctcccgcac
     1141 accgttgaag atcgtgcgcc ggcgcggacg gtgggtcgcc tacaccaaga gcgccgacgg
     1201 cgtaacgacc atccttgccc aaatgcgcct caacgacgcg gtcctcgagt acgaggcaag
     1261 ggccgttctc ggcgaggcca aggccaacgc gaaccgcgtc accaacttcg acgcggccaa
     1321 cgccgggcgc acggcgacgg ccgcagcccg ccagcagaag gcctttcgag ccatcgaccc
     1381 cgagcatcta tcacccgcgc tccgtgagat gctggagctg cggctcgagt accccgacgc
     1441 ctcccttgcc gatctagccc acctcggaaa tctctccaag agcgcgacca atcacaggtt
     1501 gaggagactc gttagcatgt cagcacgctc cggctgacgc ttccgcgtgt aagcacgctg
     1561 cgcccttcgg cggcgtgctg cataatcttc aggcattccg ggcagaataa gaacctgtgg
     1621 caagctagag acaggagaga agagtggccg taagagtagg cgtaaatggt ttcggccgga
     1681 tcgggatgct gttcaccaaa gccgcgatgg accgcggcga catcgaggtc gtggcggcta
     1741 acgacctgat gcctttcgat agtctggcga accttttcaa gcacgactcg acccacggaa
     1801 tctggcacga ggacgtctcc tacgacggca acgtgctgca cgtgggcgac catgagatca
     1861 agaccttcgc ggagcgggac ccggcccaga taccctgggg cgacgtcggc gccgatatag
     1921 tggtcgaatc aacgggtgtc tttacaagcc gcgacggggc agcggcccac atcgaaggcg
     1981 gggcgaagaa ggtcatcatc tcggccccgg gcaagggtgt cgacgccacc ttcgtctata
     2041 aggttaatca cgaggattat gacccccagg cccacgagat cgtctccaat gcctcgtgca
     2101 cgaccaactg cattatcccg atggtcaagg tgatccagga caacttcggc atcgagtcgg
     2161 gttacatgac caccgtgcac gcctacacca acgatcagag cctcctcgac gcggcccaca
     2221 aggacccgag gcgcgctcgc tcggcaccca acaacatcgt cccgacctcc accggcgcgg
     2281 ctcggactgc gggtgtgatc taccccgagc tacagggcaa ggtcgacggg atgtctatgc
     2341 gcgtccccgt tcccgacggc tccataacgg acttcgtggc ccgcatctcc agcgaagcgt
     2401 ccgccgatga cgtgaacgcc gccttccggt cggccgccga cggagagctc tcagacatcc
     2461 tcgagtatgc ggaagccccc ctggtctcca gcgacatcat aggcaacaaa gcctcgtgcg
     2521 tcttcgattc gcagctcacc atgtctaacg gcggtaccgt caaggtgctc ggttggtacg
     2581 acaacgagtg gggctactcg aacaggacgt gcgacctggc ggcatacatg ggagacaagc
     2641 tctaagagca agtcagcccg ccgcctgcgg cggctggtca gcacgctcag accttaaggc
     2701 cttcgcttgt cagcacggcg gcttcgctgc ctttcagcca gtcagcaagg gcttggcact
     2761 cgaaagctga cgagctgaaa tgctgccgac gaaggaggca ccatgaacaa acggagcgtc
     2821 agagacctag acgtgcggga caaacgggta ctggtgcgcg tagacttcaa cgtccctgtc
     2881 aaggatggtg aggtcacaga cgacactcgt atccggcgcg cgctgccgac gatcaggtac
     2941 ctcctcgagg aaggggctcg tccgatattg atctcccacc tcggccgtcc caagggtgag
     3001 cctgatccga agtacgccat ggacccggtc gccgcgaggc tccaggagtt gctgggtgaa
     3061 cctgtaaaga agctcgacgc cgccgtcggc cccgaggttg aagaggccct cgaggattgg
     3121 gacggaaggg gcatagtgct gctcgagaac tccaggttct atccaggcga gaccagtaac
     3181 gatcctgagt tcgcagatca gctcgccgcc ctggccgacc tttacgtaaa tgacgcgttc
     3241 ggcgctgccc acagggcgca cgccacgacc gtcggagtgg ctgagaggct gccggcggcc
     3301 gctgggctct tgatggaaga ggagatcgac tacctcgata aggttctcaa agaccccgag
     3361 cgccccttcg gcgccatcct cgggggggca aaggtctcgg acaagctcgg cgttatagag
     3421 agcctgctag ggaccgcgga ctcattgctc atcgggggcg ccatgtgctt cacgttcttc
     3481 aaggcccagg gatatgagat cgggaactcc ctcgtcgagg acgactacct ggaggaggcg
     3541 aagcgcctaa tggaggaggc cggcgacagg ctggttctgc ccgtggatgt tgtggtcgcc
     3601 gaagctatgg aggaggacgc cgagtcagag acggttcccg tggacggcat cccggcgggc
     3661 aagatgggcc tcgacatagg cccaaagacc gtggacctct tctcggaaca catctcgggt
     3721 gctagcacta ttttctggaa cggtcctatg ggcgtcttcg agttcgacgc cttcgcgaag
     3781 ggcactgagg gcgtggccag ggccgtcgcc gagagtgggg cgacgagcgt ggttggcggc
     3841 ggtgactctg ttgccgccgt caacaagctt gggctcgaag accggatgag ccacatctcg
     3901 accggcgggg gggcttcgct cgagtatgta gaaggcaagg agctgcctgg catagcagtc
     3961 ctgcccgaca aatagaacag ggaggagatc atggcccgac gcccgatgat ggccgcgaac
     4021 tggaagatga acaagaccgt cagggaggcc caggagtata cggccgccct cttgccgcgc
     4081 gccgccgatg cagaggagga ggtagacgtg gccgtctgcg ccccctttac ttgtttgcgc
     4141 gaggtggaca ggatggccgg ggactctgcg gtcatcgcag gcgcgcagaa cttctactac
     4201 gaggactggg gggcctttac aggagaggta tctgcgccta tgctcctcga cgtaggggcc
     4261 ggtgcggtca tcgtgggcca ctctgagcgg cgcgaagttt tcggcgagac cgacgagatg
     4321 gtcgccagga agaccaggcg cgccgtggag gccgatatcc tgcccatcgt ctgcgtcgga
     4381 gagaccaagg aggaacgcga cgcaggaggt atgtgggaga aggtctcggg tcaggtcggg
     4441 cgggtcgtcg aggagctaga agacgcaggc ggtgagagtg tcgtcttcgc ctacgagccg
     4501 atatgggcca tagggacagg cgataccgcc acccccgagg atgcgcagga tgccatcggt
     4561 aggatcagag atctgctgag ggagtcgaaa ggagacgact tctcagggag tgtccgtatc
     4621 ctctacggtg gctccgtcaa gccggagaac gcggctgaga tcatggccca ggaggacatc
     4681 gacggcgcac ttgtcggcgg ggcgagcctg gaggtagaat cgttcgtcga gctgataggg
     4741 gcagcgcaga acgccgtcgc gcgaacagag cagtagggcg tgtggtaagg tagacgtatg
     4801 ctgtactaca tcgtcgcttt ctttcaagtc gtgttctgca tagccttggt gggcctgatc
     4861 ctcctccact cgggaaaggg aggcgggctc tcctcctcgc tcggcggcgg aatgggcggt
     4921 accttctcgg gtaccaccat catggagaaa aacctgacca ggttgaccct gatcgtcgta
     4981 ggactcttcc tgctgaccaa catcaccctg ttcttcatgg gatagagcat ttcagcacgc
     5041 cgcttcgcgg ctctaagcaa tttagccagg gcattcgcgt gcgtaagttg actaactgaa
     5101 gtgctgatcg accgaaggga gcgaacatgc ccgaagcgtg ctgagagcgc atgccgaagg
     5161 ctagagcggg ctgacaagcc aagtagaagg actcgcccgg ggaggatggg gagaacaggc
     5221 gagtccacac taattataca ccccaaggta tctctgggcg tatactaggt tacacggtca
     5281 cagcgacgtg catgaggctt ctgagcctta tgtactacga gacatcaaca agtcagcacg
     5341 gcggctcaac cgccggtcgg ccagtcagcc aggtctcgca cactgaaatg ctgacaagcg
     5401 tggaatgtgc gtggtgtcgg aggggggact cgaaccccca cccccttatc ggggactagg
     5461 ccctcaacct agcgcgtcta ccaattccgc cactccgaca gggtagcccg ctgagtatac
     5521 gagagacata tagtggggtc aacgccgcac gcccctctcc actatgctaa gttcacctga
     5581 tgcgcagaag gcacttaatc ctgatatccc tcgcagggtt ggccagcttt gtcgccctac
     5641 tcttgcggaa gggggagaag aggaccgttc gaggcggatg gcccgtcaac ctggcacaca
     5701 gaggcgcctc ggcccttgcc ccggagaaca ccatcgaggc attccggctt gcggtgcagg
     5761 acggagctgg cggcctcgag ctcgacgtcc acatgacccg cgacgggcac atcgtcgtga
     5821 tccacgacgc gaccgtggac cggaccacga acgggtccgg cgccgtctcg gagatgacct
     5881 tcgacgaact tcgcaggttc gatgccggac acgacttcag taccgacggc ggacccaccc
     5941 gtccttaccg tggcaggggc gttcaggtgc cgacgctcgg ggaggtcctg gaggagttcc
     6001 ccggcgtcgc tgtcaacatc gacataaagg ccggtactcc tcgtatcgag gagactctct
     6061 tcagagtctt gcgcgaagcg gatgccttga gacgtgtcct cgtggtctcg atcccccacg
     6121 ctagagtgaa gcggttccgt aaggtctcag gcggccacgt ctccacgggg gcttcgaggc
     6181 gggagatagg gatcttcttc atttcgagca ggctcagcct ggagcgtctc gtgcgccctg
     6241 cctacgacgc ggtgcaggtt cccctcaggc acagaggaat acgggttgtg accccgcgct
     6301 tcgtccgagc ggcgcatgca aggggcgtga gggtggacgc gtggaccata aaccaggcgg
     6361 acgagatgcg ccgtctgctc gacttcgggg tcgacgtgat catgaccgac cggccaggca
     6421 cgctggctga agtgctgaaa aatcgcccca cgaccctcac gccgccatga gcagcacgat
     6481 ggaagccacg ccgaagagcg tgtaagcggt gccgaggggc aggaagtacc tgggctcttc
     6541 acgcaggggt gtgaggtact gggcgtaggg gagtatgcgc atcgcgcgcc tgagtacagc
     6601 gttgtactcg tagcggaaca ggatcatgtg cagcccgttg gcgaccgtga ccaggcaaaa
     6661 cggcagggcc cagagcttcg aaggatagag cttcaaggca gggggcgtga cgaagatgca
     6721 gagcagccag atcacctgga tggtcacgta gctcacccag gcggccgtga tcctgtatct
     6781 gtgcttctcg tcgatggtga gattctaatg tagggacagg cgcgagagca ttcgtttcgc
     6841 cctgattgta aacttctcac cacgtggatg aggttgaatt cggcctgtag aacggtatat
     6901 agagtaggag aaacatgatc agtgaatcgc tcgtcaaaga tccttccctc gcccctgagg
     6961 gacaccgcaa gatcgattgg gtcgcccagc acgcgccggt tttgaacaag atcgcgggcg
     7021 agcaactcgc cgatggttcg ctgcgggggc gcaaggtcgc gatgaccatc cacctggagg
     7081 cgaagacggc ttatctggcc ttgctcctgt ccgaagccgg ggccgacgtt accgtctccg
     7141 ggagcaa
//
LOCUS       DASTKM010000330         5010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_495942, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000330 DASTKM010000000
VERSION     DASTKM010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495942"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..486
                     /locus_tag="VFQ10_13150"
     CDS             49..486
                     /locus_tag="VFQ10_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966335.1"
                     /translation="MAAADDVHELIEQYYRVQREFLRGNPQPVKNLFSHREDVTLANP
                     YGPPVRGWQKVDKAIEHAASLRRDGTFLGWQIVGKYVSAELAYVVQIEWAEAKIGARE
                     EISPLAVRATMIFRPEEDGKWKLVHRHADPITTPQPAESVIQE"
     gene            complement(1103..1480)
                     /locus_tag="VFQ10_13155"
     CDS             complement(1103..1480)
                     /locus_tag="VFQ10_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9966336.1"
                     /translation="MHGGGTLMERLLLVEHNPLFREALALLLEWQTGLSSTHAESLAE
                     AKGVLEEANQKKPACIIVDLDLPDGEGNELLKQLDGIPVLALIKGRSLQRRAEAMGLG
                     ADELLRTGSVEKIAAAVERLIGH"
     gene            2008..2547
                     /locus_tag="VFQ10_13160"
     CDS             2008..2547
                     /locus_tag="VFQ10_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966337.1"
                     /translation="MQKWKGMPGYPEAADGLMRGLLGVPEPSSRLPRAGVLESPHLWP
                     ELRGFYGPEGPLNTNLRLWGTYAGELEVRVEGKHLVLRALAGPLRKGGRLYPIDSLDP
                     LAFEVLVEGQAHPIVFGREREGGRVNRLCVGFDRLTRRPRAGSVRFKAQADAGMIAAT
                     GLAVATWIGVRVLRVPKER"
     gene            2785..3120
                     /locus_tag="VFQ10_13165"
     CDS             2785..3120
                     /locus_tag="VFQ10_13165"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9966338.1"
                     /translation="MDLAGTAAAAGERTGVIWTLLESNDLNANLVRFEAGGGVGEHIN
                     DEVDVVFVGVSGTGSVLTNGEEHRLSAGTLVLVRRGARRSILSHSEGFSYLTIHRRRG
                     PLQIESEAR"
     gene            complement(3176..3376)
                     /locus_tag="VFQ10_13170"
     CDS             complement(3176..3376)
                     /locus_tag="VFQ10_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966339.1"
                     /translation="MWWADLLWGFWNGLTAWIVLVVHIFGGWEQFPFYNEARSGNWYD
                     FGFLLGAGSPLLGAVGGRGRRG"
     gene            3583..4680
                     /locus_tag="VFQ10_13175"
     CDS             3583..4680
                     /locus_tag="VFQ10_13175"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HET9966340.1"
                     /translation="MAVRDYEAVFVGGGLAAMLLVRELGSALSGRVAVVDPCPPQEKP
                     TVHWSYWSHGPTLYDRFAVGSWQKARVVQAPAESIAPYTLRLVRSTDVFDHIAEELRT
                     VPIEWLRTSARSIERREDGTYEVITDAGAVRAGWVFDSVPDIEPAFPSLHRPRAVLSG
                     TGVSVTADRPVFDAAAATLFDPLDERSFAYLLPLSPDEALLESAMFDSAIQKEDETPL
                     LRYLQSRYPGATFTVTHAEHGHIPLGFAPSQTAGPRHVLLGTKRGLVKPSAGYGVVRM
                     AEESKRLAKLWKQGRPLPPSQRSSLLWRLLDVGFLQLAAHDPRRPLALLRNVMHTVPL
                     VQSLRFIDEDLPPRQLAAVFRSALPAVLGKP"
BASE COUNT         1025 a         1511 c         1517 g          957 t
ORIGIN      
        1 gtccttaggg aaaggagcat ctgccaacga tggaggtgat ctctacaaat ggccgctgca
       61 gatgatgttc acgaactaat cgagcaatat taccgagtgc agagagagtt cttgagaggc
      121 aacccccagc ctgtgaagaa cctcttctcc cacagagaag atgtgaccct cgctaacccc
      181 tatggccctc ccgtgcgtgg gtggcagaag gtcgataagg ccatagagca tgccgcatcg
      241 ttacgcagag atgggacgtt cttaggttgg cagatcgtag ggaagtacgt gagtgctgag
      301 ctagcctacg ttgtacagat agagtgggca gaggccaaga ttggcgcaag ggaggagatc
      361 agcccgttag ctgtacgggc tacgatgatc tttagacctg aagaagacgg taagtggaag
      421 cttgtgcatc gccacgcgga ccccataacc acccctcagc cagcggaatc ggtgatccag
      481 gagtagacct actcttagag agacttcgga gaacttcgtc atggcgaagt tcggcatagc
      541 cccgatccag acggaccaag aacgccagcc agcaagcaag aagagcgggc ggcccggtaa
      601 aaggggaaag gaggaaagag tggaccgccc gaacgctgtg acccctataa ccctacgact
      661 gccaagagag ctccgcctct gccagttggc cataatcagc tataccaggt ggccatttct
      721 caatcgccgc tcagccacgg tccgataggg ccaggtgctc catcttgagt ctgtccataa
      781 tcgggtatta tgcgctgccc taggttggtg atctcgaagc gctggctagg cgttagggct
      841 tcttagagca ccggtaaact tagcagcagg cggaggggga gaatcctgcc actaggccta
      901 aggactccgg aagctctgag agaaagaccg cagtactccc aggtccaccg ataggctaca
      961 ccctcccctg tgcctcggtg ttaaacgggc gcaaaccctc ggtaaaagat aggtaaaacg
     1021 ggtaggtccg tggcgacgcg agcttcgtag aattcacctt ccacgcactc ggctgaatag
     1081 agcctagaag acggtaatag atctagtggc cgatgagccg ttccaccgct gcggctatct
     1141 tctcgaccga ccctgtcctt agaagctcgt ccgcccccag tcccatcgcc tcggcccggc
     1201 gttgcaggct gcggcccttg atgagcgcta gcaccggtat cccatcaagt tgcttgagta
     1261 gctcgtttcc ctctccatct ggtaaatcga ggtcgactat gatgcaagcg ggcttcttct
     1321 ggttcgcttc ctccaagaca ccctttgcct cggcgagaga ctccgcatga gtgctactca
     1381 agcccgtttg ccattcgagc aggagtgcca gggcctctcg aaagaggggg ttgtgctcga
     1441 cgagcaatag ccgctccatc agcgtacccc caccgtgcac atcgtgttgc tctgtggaag
     1501 gtagctggcc ccggtctaga aggagagcag gtagccagtc ctgcgccgac gcgcgaagct
     1561 cgcgcactag acacagttgt aacatatctt tacgtggtac ggagtcatag gggcaagggg
     1621 caaatgcacc actttcggag ctctcgctcg acgtacttct gagaactccg ttaaggcgga
     1681 agttcggaga attccacttc taggcacttc ggcgaataaa cttgggcatt catccggtat
     1741 tcattaccct tagctatcct gcccctcgag taaataaccc gctaagggtc gcagcgtcga
     1801 tgaaaggtgg tcagtacatt ttgtgtagta acaacattgc caagttgggc gctgggtgta
     1861 gagtggtagg ggcttcggct ggccttcaaa ccgataaggg gaggaaacca tgttgcgacg
     1921 tgtaatcttg atactcagct tatctgctat cgcagcagca ttgcttgctc taggagcagg
     1981 tggggcattt gcgaaggacc ccatacagtg caaaagtgga aaggtatgcc agggtacccc
     2041 gaggccgcgg acggactgat gagagggctg ctgggcgtcc cggagccctc ctcgcggtta
     2101 cctcgcgcgg gcgtgctgga gtcgccccac ctctggcccg agcttcgtgg cttctacggt
     2161 cccgaaggtc ccctgaacac caacctgcgg ctctggggga cgtacgccgg ggagctcgag
     2221 gtccgcgtcg agggcaagca cctcgtcctg cgtgctctcg ccgggccact aagaaagggc
     2281 ggaaggctct acccgatcga ctccttggac ccactggcgt tcgaggttct cgtcgagggg
     2341 caggcgcacc ccatcgtctt cggacgcgag cgggaggggg gccgcgtgaa cagattgtgc
     2401 gtcgggttcg accggctgac caggcgcccg agggccggga gcgtgcgctt caaggcgcag
     2461 gccgacgcgg ggatgattgc ggcgacgggg ttggccgtag cgacgtggat aggcgtaagg
     2521 gtactgcgag tgccgaagga acgttgaggt ccgtggacgg tccaagacga tacctcagga
     2581 ttcctctacg gctcgccacc atagccaatg cgcaccataa gctccgagag gtgggctttc
     2641 gcctccctca cccttacctt ctcggcccca tttacctcca acgcatcgaa cagatcatct
     2701 cgacgagatt agcacccgca ctgcacgcct gcgcaaacag aagagattat ctatgacgcg
     2761 cagcccgtat agaggggata gctgatggat ctggccggga cggcagcggc tgccggcgag
     2821 cggaccggag tgatctggac cttgttggag agcaacgacc tgaacgccaa ccttgtgcgc
     2881 ttcgaggccg gagggggcgt gggtgagcac atcaacgacg aggtggacgt agtctttgtc
     2941 ggcgtctcgg gcaccgggtc agttctcacg aacggtgagg aacatcgcct gtcagccggg
     3001 acgctggtgt tggtacgcag gggtgcgagg aggtcgatcc tctctcactc tgagggcttt
     3061 tcctacctca cgatccaccg gcggcggggt cctctacaga tagaatcgga ggcacgatga
     3121 agtaggagat cagctcgaag cccacattcc acttcgaaga caggtacacg atctgctaac
     3181 ctcgccggcc tctcccgccc accgcgccca gaagggggga tccggcgccg agcaagaacc
     3241 cgaagtcgta ccagttcccg ctcctcgcct cattgtagaa agggaactgc tcccacccac
     3301 cgaagatgtg cacgacgagg acgatccacg ccgtaaggcc gttccagaag ccccacaaca
     3361 gatctgccca ccacacgatg tttacctcct ctatgaccgg cgcagtttca cttcttccga
     3421 gacgactcgt cttgcgtccg aacgcgaagt atgacatggg accgtccaca tcttccttgc
     3481 catgcgcgca cctactcgac gccggatccg ccttcgatta tatgactgat ccagattcct
     3541 cagagttccc cctccgcggt gggcaacgta tcatggtggc ctgtggctgt tcgagactac
     3601 gaggcggtct tcgtcggcgg tggcctggct gccatgctgc ttgtgcgcga gcttggatcg
     3661 gcattgtccg gacgggtcgc cgttgtcgac ccgtgcccgc cgcaggaaaa gccgacggtc
     3721 cactggagct actggagcca cggtccgacc ctctacgacc ggttcgccgt tggaagctgg
     3781 cagaaggcca gggtagtgca ggcgccggcc gagtctattg ccccgtacac cctgcgtctg
     3841 gtgcgctcga ccgacgtgtt cgaccatatc gccgaggagt tgagaaccgt gcctatcgag
     3901 tggctgcgta cctcggcacg ttcgatcgaa cggcgggagg acggaactta cgaggtcatc
     3961 acggacgccg gtgcggtgcg tgccggatgg gtattcgaca gcgtccctga catcgagccc
     4021 gcttttccgt ccttacaccg acccagggcg gtgcttagcg gcaccggcgt cagcgtcaca
     4081 gccgaccgac cggtgtttga cgcggcggcc gccaccctgt tcgacccgct cgacgaacgg
     4141 tccttcgcct acttgctgcc cttgagcccc gacgaagccc tgctcgagtc ggccatgttc
     4201 gactcggcca tccagaaaga ggacgagacg ccgctactgc ggtacctgca gtcccgatat
     4261 ccgggggcca ccttcaccgt tacccacgcg gagcacggcc acataccgct cgggttcgcg
     4321 ccttcgcaaa ccgctggccc ccggcacgtg ttattgggca ccaagcgcgg cctcgtcaag
     4381 cccagcgccg gctacggcgt ggtccgcatg gccgaggaga gcaaacgact ggcgaagctc
     4441 tggaagcagg gcaggccgct gccgccgagc caacggtcct ccctgttgtg gcggctcctc
     4501 gacgtggggt tcttgcaact cgccgcgcac gaccccaggc ggcctctggc cctgctgcgt
     4561 aacgtcatgc acaccgttcc cctcgtccaa tcgctgcgct tcatagatga ggatcttcca
     4621 ccgcgacagc tggcagcagt cttcagatct gcgctcccgg ccgtgctcgg aaaaccgtag
     4681 cgccagcgag ttccgggagc cacgcgtcag cggaccacgg agagggcgat atctccagca
     4741 gacccaatgg cggtatacgc gtacggttcg agatgcctac tgccagggac ggagggtctt
     4801 caggagaatc gcgggtagac cagggcgacg aacagacccg gggcgaccat gactagcatc
     4861 acgttgaccc cgagggccct ttgctactcc tttgctcctt atcgttggtc cacctttgcc
     4921 cttcaacaac catgtaagat tgcaacgaaa tagtcgcgac tcgtgggatg ggtgctcact
     4981 aaacccgaga aggtttggcg agaaccaggg
//
LOCUS       DASTKM010000331        22576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_143994, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000331 DASTKM010000000
VERSION     DASTKM010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 22576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22576
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143994"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..294
                     /locus_tag="VFQ10_13180"
     CDS             49..294
                     /locus_tag="VFQ10_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966341.1"
                     /translation="MEVKMLPKFDYYIDKSDPDIVVLRRQDGSFVAAFSARGATRESI
                     AEAAKEDYFKLIEVNAGSLNLRESNDQQRKGVRRSTA"
     gene            394..1554
                     /locus_tag="VFQ10_13185"
     CDS             394..1554
                     /locus_tag="VFQ10_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966342.1"
                     /translation="MVLTVGLAVVLLWLLAGCRVADQRHNAEDPDSGLRASSAGNGSS
                     GGDQHTEISNGKIVFIRHSDIHVMNSDGTGKTNLTRTKAGVGVWSPNGKKIAFFARSA
                     GERPLDIYVVNADGTEETNLTRTQTSSEGAPSWSPDSKKITYLRGSDPSGEIYNDIYV
                     MNSDGTSQTRLIRATDTKDFEVGFESPVWSPDGENIAFLRTTRVDLVNSSPSSAVPAT
                     GPSGLYVMKPDGTGLKKLSKEVPYAQTPLWSPDGEEIAFSGAGEKKYIVNADGTELRE
                     LMPNVGDHISSYSWSPNGEKIAFAAVHYRGELDIYEIDADGTGQTNLTSTKTIIEDEP
                     SWSPDGKQIAFTRADAGNFFVDQDVYVMDADGTGRTRLANNASSPSFAPRDR"
     gene            complement(3109..3288)
                     /locus_tag="VFQ10_13190"
     CDS             complement(3109..3288)
                     /locus_tag="VFQ10_13190"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HET9966343.1"
                     /translation="MIDLRAFRDRLRANPEMMEAYVQRKRDIIEGGTTDALDYSIVKG
                     SFVREALAKWRSADG"
     gene            complement(3536..3781)
                     /locus_tag="VFQ10_13195"
     CDS             complement(3536..3781)
                     /locus_tag="VFQ10_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966344.1"
                     /translation="MERDDAEFRAANERITMMAEELRKAELVRDRLEGLARLMGSYPE
                     GHDMRARLETLHVDRALEGVDEDIRLLTDALQHPRGT"
     gene            4346..4654
                     /locus_tag="VFQ10_13200"
     CDS             4346..4654
                     /locus_tag="VFQ10_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966345.1"
                     /translation="MSTERTTIRARARSDGLRLLRTINQTQAHGEEGARADPTRAAHQ
                     AGLDVGSERYQDAMAYLIEQAALLGDAHMAFGDDVGDQHPHGYASYFFTRRALTLLER
                     "
     gene            4997..6424
                     /locus_tag="VFQ10_13205"
     CDS             4997..6424
                     /locus_tag="VFQ10_13205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9966346.1"
                     /translation="MQPSGFGESSGPTHEILRESEERFRLLVEGVKDYAIFMLDADGY
                     ITTWNLGAQRIKGYESEEIIGEHFSIFYTDEDVERGHPEEELRIAAADGSYEEEGIRV
                     RKDGSHFWASVLITALRNEEGHLRGFAKVTRDITARKVAEERERLLTREPAALERATD
                     ILESISDAFFAVDEECRFTYVNGKAEQFWGRSRDELLGKNIWEEFPQAVGSEYYRQIQ
                     RAMEEDVTTEFEATFPVLGVWVAGRAYPSREGLSVYFQDVTERKLAEESLRRVTEAER
                     KRIARDLHDSVLQDFSYTTAALGMMVLQAKDATSRKQLQAAVDAVRRGAQGLREVVNN
                     LRLEDEEDRPFPEIVGALVRRSRTMAPALEISLEVGEHVPPAPLGETGTQVSRIIQEA
                     LTNARKHSEAMRVSVSVKMDRGDLHVEVSDDGRGFGSESSPGVGLSSMRERAAIIGRE
                     LEIESEAEQGTRVRLRAPLPRGVLE"
     gene            6421..6804
                     /locus_tag="VFQ10_13210"
     CDS             6421..6804
                     /locus_tag="VFQ10_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9966347.1"
                     /translation="MSGEDGTKQIRVMLVEDHPDFRDLMEVLLGSQSDINLVAQAGSL
                     AEARNHAVRFDFDVAVLDLGLPDGDGVDLIADLRQANPDVGVLILSASLDAASFERAT
                     EADEIMDKLAPLDEVLGTLRRLGNA"
     gene            7122..7652
                     /locus_tag="VFQ10_13215"
     CDS             7122..7652
                     /locus_tag="VFQ10_13215"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9966348.1"
                     /translation="MRRVRYVVAMSLDGYIAGPNGEADWIIMDPDIDFGALFEQFDTF
                     LLGRRTFEGMAGTGGGSQSGIQTIVFSRTLRQEDYPNVSIVSENPEHALTDLRSKPGK
                     DIWLFGGGSLFRSLLEAQLVDAIEVSVIPVLLGEGIPLLPPNPSSERFELKLTSSRTF
                     KTGIVSLEYDVAYGLA"
     gene            7837..8511
                     /locus_tag="VFQ10_13220"
     CDS             7837..8511
                     /locus_tag="VFQ10_13220"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HET9966349.1"
                     /translation="MDNDTVLVRLGGLCGILFVLLLVPGVFAARPDVPDASLSAPQML
                     DYFDGKQGALLVANGLSFIFATFFFVWFLGVLHAVLRSAEGEQSGLSSAALAGGLMFV
                     ALESAGASVEIVHPASTSRFENFQPDAQLVFLSQALSGWLYSFAWVGLAVLLSATSLV
                     ALRTGFLPRWLAWAGFLAALVAVLKFLLPYATLALLWTLAVSVLMIIGSVSSSGVGTR
                     RLGRAS"
     gene            complement(8601..8861)
                     /locus_tag="VFQ10_13225"
     CDS             complement(8601..8861)
                     /locus_tag="VFQ10_13225"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9966350.1"
                     /translation="MAQLRDGEITSFEVIVKYVTAELAYTVWIERQQARLGGRQDITP
                     FTLRVTMIFRPEDGEWKIVHRHADSITTPQPVESVIQEQLNP"
     gene            complement(9093..10268)
                     /locus_tag="VFQ10_13230"
     CDS             complement(9093..10268)
                     /locus_tag="VFQ10_13230"
                     /inference="COORDINATES: protein motif:HMM:NF020069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9966351.1"
                     /translation="MTTVKKVLQRGLVATAIMLPLILVFYSPADAQARVVNIPCGQDI
                     DATINADPNGTATGFVLGADCTFVASATIVPSDGDEVACAVAPTFEQRGPAFDPTTRC
                     TVAGNDSVENVFKPTGQGGSMVTVHFEGIKITGGNFTGSSGSGAAIAEGKMTDTSSHY
                     GIEVMDNDAAGILSAKGTFERVELTNNTRDPKALGFIAAGMKARNEVEVKNSYVHDTQ
                     GNGLWCDNFCHDSDSHPNGFWVHDNLVVNNGRAGIRFEEVGGVPTAGEALIENNEVHD
                     NSLHANRGGISVRDAQDATIRNNHFGAATIGGVAYLPNSHDVAITASDSGRSDRPNLS
                     NIRVVNNALDGETVKGCELPNTVVACAGDTRWCANIVDRVYELLRTYLPLTRVNNVG"
     gene            10689..11132
                     /locus_tag="VFQ10_13235"
     CDS             10689..11132
                     /locus_tag="VFQ10_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966352.1"
                     /translation="MIGFMSVEIQAEADFSRARRRAFFGRIAARLRRECSWLLAFDKI
                     HLTHNRRGLGLRDVEVSKIVGSVGRHEAFDRDFMPTKASLAERWMMVDRAFHRGLDLP
                     AVRLYKVGNSYFVEDGNHRVSVARYQGVETIEADVIEFFPLYGAH"
     gene            complement(11333..11638)
                     /locus_tag="VFQ10_13240"
     CDS             complement(11333..11638)
                     /locus_tag="VFQ10_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966353.1"
                     /translation="MEEDHKLVEDLMRLYGFEEKEARAAYHLGIAEELFEEISEEAYQ
                     RGGFPRVRGIQAWMHDEMDFGEHFHALRNQLARRVLQRDYPEGWGRRPGQSSDSRLD"
     gene            11989..12369
                     /locus_tag="VFQ10_13245"
     CDS             11989..12369
                     /locus_tag="VFQ10_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9966354.1"
                     /translation="MEERKMKIGSIVVRCYEFDKVLAFWREALHYVPREPAQDGWVVL
                     HDPEGRGPNVSLEKVPEPLEHIGKTNRVHLDLYTTDQEGEVERLVGMGARRYPQEYRP
                     DDDFIVLEDPDGNRFCVVQVPDGG"
     gene            12448..12768
                     /locus_tag="VFQ10_13250"
     CDS             12448..12768
                     /locus_tag="VFQ10_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9966355.1"
                     /translation="MAEYLPEVYQHFERRYPTVKEAFDALGSAEHDAGPLGERERRLI
                     KLAIAVGAQSEGGVRSHVRKLLGVGVSEAEILHTIVLAITTIGFPATNAALSWAEEVL
                     SEEG"
     gene            12963..13904
                     /locus_tag="VFQ10_13255"
     CDS             12963..13904
                     /locus_tag="VFQ10_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9966356.1"
                     /translation="MNIFPTKVLVATDGSREAHLATRTAAELAAKTGSELHVVHVFGI
                     TPWYPAYPEATAFNGAELEDPVLEEDLQRITEQRARELLDAEVEKLRSVGGTPAQAHL
                     VEGGVPQEIVELAEELGAGLIVMGSRGHGGIRRALMGSVSDSVVRHAHCPVLVVRNGE
                     RERDYLPGRILLAVDGSEEASAAARTAVELADRMDSELHVVYVGEVRPVYHPERRGYL
                     ARYEEIQEEARQLLEEQVDEVKSAGGTVTRAHLRLGHPDEEIVGLGEEIGADLIVTGS
                     RGLGGMRRVLMGSASDSVVRHAHCPVMVVRPEKEQTV"
     gene            complement(13985..14413)
                     /locus_tag="VFQ10_13260"
     CDS             complement(13985..14413)
                     /locus_tag="VFQ10_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9966357.1"
                     /translation="MSIFPTKILVATDGSREAQLAASTAADLANTTNSELHVVHVGQF
                     EPTFLAQTEVEPAELEREAQRLLDEQVRTVEEAGGTVKEAHLRLGRADEEILDLAHSL
                     GIGMIVMGSRGRGKIRRVLMGSVSDSVVRHAHSPVTIVRE"
     gene            complement(14818..15066)
                     /locus_tag="VFQ10_13265"
     CDS             complement(14818..15066)
                     /locus_tag="VFQ10_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966358.1"
                     /translation="MKRPGVWCRGGELRPEYGSGEVDIRLVVSVLGLVFVVLQCAVMV
                     VVPVGDGRLMKGGLLQGGVHPTEPGQRCNGLRDDNDQR"
     gene            15308..15946
                     /locus_tag="VFQ10_13270"
     CDS             15308..15946
                     /locus_tag="VFQ10_13270"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HET9966359.1"
                     /translation="MKRPSFVVGGILGLSAMLAVSAPAWAHVVVSPEVVTAGDYETLT
                     VSVPTEKEIPTTKIRVVVPEGFLLSGVQPVPGWEHTFEEDGGVVRAVTFTGGEIRPRE
                     FQQFLLQAQVPEEPGEYPWKAIQTYEDGSVVEWTGPPDSEEPASVIEVVSGGSDHPQA
                     SPQPSEASASQKAAGGETKVLADTGGTNPALYAGLGFVGLVVSALLARRLLG"
     gene            15949..17367
                     /locus_tag="VFQ10_13275"
     CDS             15949..17367
                     /locus_tag="VFQ10_13275"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HET9966360.1"
                     /translation="MAPRSSVGRLAIVSWLAATWLLLACAPALAHANLVGASPRPGGE
                     VSKPPERVELRFSEPVDAEFDPVVVRTVSGARVDAHNAHVDPEDARVVLADLERVPEG
                     SYTVKWRVTSIDGHVVDGRYAFAVAAAGEDRPSEDAGDRATTAEAAGAHSGHHGGHHG
                     GGAGRVASTDRRDPVPEPAAGRAARIEHGLALAATAFLAGLAPFAVLVWLPASRQSGV
                     GRAAIRSFGVLAGGLLLALALAGVGELSSYAVRASGDPLSTELFWQTLFDSRVGALWL
                     TRLSLALLAAAVIATAARSGRTWPWWAAIGASGMLMITLTGLSHAAATGRLLPLLADW
                     THAVAATVWMGGLLGFAVALCSRAFRDLAPDSRMKLRDRAVRRFSAVAKCAVVVPACT
                     SLYAAVLHIPNPQALLATPYGRALIAKLALLSLLLAAGASNLLLRGRGPFGRLVVLEL
                     LLALALFVATGFLTSLPPASGA"
     gene            complement(17452..17574)
                     /locus_tag="VFQ10_13280"
     CDS             complement(17452..17574)
                     /locus_tag="VFQ10_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966361.1"
                     /translation="MRKQKVLFLCTQNSVRSQMAEGFLRHLYSPSEGALSGGGA"
     gene            complement(17577..18011)
                     /locus_tag="VFQ10_13285"
     CDS             complement(17577..18011)
                     /locus_tag="VFQ10_13285"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET9966362.1"
                     /translation="MAAELGERARVLFLCTHNSARSQMAEGMLRHLGGERFEVHSAGT
                     EATHVRPLAIRAMDEIGIDISGQESKTLERYLEESFDYVITVCDDVNEACPFFPGAAN
                     RLHWSFEDPSRARGSEEGQLAVFRSVRDRIKDRVQAELVNGG"
     gene            complement(18001..18417)
                     /locus_tag="VFQ10_13290"
     CDS             complement(18001..18417)
                     /locus_tag="VFQ10_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9966363.1"
                     /translation="MNTRVLGVVEKYGVRRDGPEAGLDRLVALGSALSDPIRVRMLSM
                     MAEGRSCCDLPDLGVPVEAEDEGICVCEFEDQFGMGQSKVSYHMKKLKDAGLVREERR
                     GKWSFYTLDREAAGRLLGEAADRLLPGPEKRSVGGC"
     gene            complement(18414..19283)
                     /gene="arsM"
                     /locus_tag="VFQ10_13295"
     CDS             complement(18414..19283)
                     /gene="arsM"
                     /locus_tag="VFQ10_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HET9966364.1"
                     /translation="MEEQLRDRVKERYAGAARSVLGGAGSSSCCGSAGERGLDSEAFG
                     VDWTGGGYSAEELEGLPQAAGAASLGCGNPTSLATLSAGEVVLDLGSGGGIDVLLSAR
                     RVSPGGKAYGLDMTDEMLELAEKNRAEAGVENVQFLKGHIEDIPLPEDHVDVVISNCV
                     INLSADKPRVISEAFRVLKPGGRFAVSDMVFLGDKSKLPNELVRSMEAWSGCVSGALE
                     KDEYEKMLGEAGFEDVSVEVTHTYEEDFAATSECCGGSGCCDDTTVATAAATEVPLAS
                     AFIRGRKPAGRTL"
     gene            19493..19633
                     /locus_tag="VFQ10_13300"
     CDS             19493..19633
                     /locus_tag="VFQ10_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966365.1"
                     /translation="MEEIEAIEQNLEAIKQEHGLDQASTIERAGVSFGGVQDPVFVER
                     GG"
     gene            19638..19817
                     /locus_tag="VFQ10_13305"
     CDS             19638..19817
                     /locus_tag="VFQ10_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9966366.1"
                     /translation="MEDKNRMKVTITEAAEYLGLTRTTVQDMVERGVLKADKFAGAVH
                     IPREEVERIERETAP"
     gene            complement(20465..21322)
                     /locus_tag="VFQ10_13310"
     CDS             complement(20465..21322)
                     /locus_tag="VFQ10_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET9966367.1"
                     /translation="MGRGISATSGDVIRLRGGALLVIGVGLVFVALSFTGAAAVYAMR
                     HDGQGQTTGSHAPVSVRFSEPHTSVGVRLAEPLDVAPEKEPVVKSPSAAQPSGVEAPA
                     PRATKGASQRNESEIRGARPPADTSTLVNMLTCDGGTFALNVAEKRMFDLHNSTRRAH
                     GRRALCLSPVLTQAARSRSQDMLNREYFSHYTPDGVTVIDQLERRGYYSYDPGDYHAL
                     GENIAMGGDGRDEDTPEHLFTEFMHSEGHRANILRAAFVEVGVGARSGTYLGYDDPST
                     VYTVVFGGR"
     gene            complement(21286..21411)
                     /locus_tag="VFQ10_13315"
     CDS             complement(21286..21411)
                     /locus_tag="VFQ10_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966368.1"
                     /translation="MESALISTQAERERVYSAEYDEQGRYDIRRWVEEFPLLQGM"
     gene            complement(21517..21918)
                     /locus_tag="VFQ10_13320"
     CDS             complement(21517..21918)
                     /locus_tag="VFQ10_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9966369.1"
                     /translation="MNEDEGVRRARLLIVDDHDLIRESTQLMLEGEPDLEVVGEAANG
                     HHALELCRQLRPDLVLMDVRMPEMDGLTATREIKKEMPTISVLLVSAYESEDYRREAA
                     SAGATDYILKDAERYQLLEAVHAALGERGEG"
     gene            complement(21905..>22576)
                     /locus_tag="VFQ10_13325"
     CDS             complement(21905..>22576)
                     /locus_tag="VFQ10_13325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9966370.1"
                     /translation="KLMTAHEEERRWLAYEMHDSLIQMVIAAQRYIQTFADTHPPGSK
                     VRHGELDRPLELVRRTVQEGRRLVKGLRPTTLDDFGLEIAVRQRVEELQNEGWEICYE
                     ETLGGERLPPEVEITLFRVAQEALNNVQKHARTTAVCVALTQRGRKVRLEIRDEGRGF
                     DPSAALAEQGPVERLGLSTMRERVVLLGGELEIRSKPGAGTSIVAEVSLPAAFEGRGG
                     DHERG"
BASE COUNT         4376 a         6963 c         6664 g         4573 t
ORIGIN      
        1 gcagcagtct gtccccctta ggaaagggga tgtccttagg aggtgagcat ggaggtaaag
       61 atgctcccca agttcgacta ctacatagac aagtcggatc cagacatagt cgtactgcgc
      121 cgccaagacg gctcgtttgt ggcagccttc agcgctcggg gggctacgag ggaaagcatc
      181 gcggaggccg ccaaggagga ctacttcaag ttgatcgagg tgaacgcagg ctccttgaac
      241 cttcgagaaa gcaacgacca acagagaaag ggcgtgaggc gaagtacagc gtgatccggt
      301 cggggcaacc ccctccgtcc cgaccggatc cagattggcc cggcgcacct gccaaatgat
      361 ctagctgaag cgactatgac acggcgaact accttggtgc tcacagtagg gttagccgtt
      421 gtactgcttt ggttgctggc agggtgcaga gttgccgacc agcggcacaa tgctgaagac
      481 cctgatagtg ggctgcgggc gtcgtccgcc ggtaacggta gcagcggggg cgaccagcac
      541 accgagattt cgaacggcaa gatagtcttc ataaggcact ctgacatcca cgtaatgaac
      601 tccgacggca caggcaagac caacctcacc aggacgaagg cgggtgtagg ggtgtggtct
      661 ccgaatggca agaagatagc tttcttcgcc aggtccgccg gggagcgccc cctcgatatc
      721 tacgtggtca atgccgacgg cactgaagag acgaacctga ccaggacgca gacgagctca
      781 gaaggcgccc cctcgtggtc gcccgacagc aagaagatca cctacctgag aggcagcgac
      841 cccagcggcg agatttacaa cgacatctac gtgatgaact ccgacggcac cagtcagaca
      901 aggctcatca gagccacgga cacaaaggac ttcgaggttg gcttcgaatc gccggtatgg
      961 tcgcccgatg gcgagaatat agccttcttg aggacaacca gggtagatct tgtcaatagc
     1021 tctccttcct ccgcggtacc tgccaccgga ccgagtggcc tctacgtgat gaaacctgac
     1081 ggcacgggtc taaagaagct atccaaggag gtgccttacg cacaaactcc cctctggtct
     1141 cctgatggtg aggagatagc cttcagtggg gctggagaga agaaatacat cgtgaacgcc
     1201 gacggcacgg agctaaggga gctaatgccc aatgtagggg accacatctc ttcatactcc
     1261 tggtcaccca atggcgagaa aatagctttc gcagcagtac attaccgcgg ggagctcgat
     1321 atctatgaga tcgatgctga cggcaccggc cagaccaacc tcaccagtac caagaccatc
     1381 atagaagacg aaccctcgtg gtcgcctgat ggcaagcaaa tagcgttcac gcgagcggat
     1441 gcagggaatt ttttcgtcga tcaggatgtc tatgtgatgg acgccgacgg gaccggacga
     1501 acacgtctcg ccaacaacgc gagtagccct tctttcgcgc ctcgggatag gtagtagaaa
     1561 cgatgaggcg tcaagcgcac acgaaccagg cgctttctgg agacagccat ctttctttac
     1621 gcccctctcc ccacaaccgc ggggcaagaa catcacgtgg caggcttctc tacggccagg
     1681 ctgcggatcc tcgagagcag gacctctccc gggcagcggg tttcttggga gcctgcctct
     1741 cccgagcgca gcaccgcgcg cgccgctcac cgagagccgc gaatccgggg ccagaggcgt
     1801 ccgcgaacag ggccaggatc acaccctctg tgaggacatc atccatttgg ggtagaaaga
     1861 aatcactcat ttgcagtatg tgctcggggc ctcctgggta ctacaatctg aacaatcgcc
     1921 aagccgggcg cactctgaac cttccccctc ccagagcagc ccggcttttc tgttgcccta
     1981 tggaccgggg gcttattagt tcgacccatc ctacgcacga cccttgctct tgattacgga
     2041 atttggggac gtcatccgac agtccctcat gccactccag gctcttggct gagcacatgg
     2101 ctgctcgcat cggggtgagc cttaaagact ctaccatgga aattaacaac gtttaaaggc
     2161 cggggtgtta gattcggtag tccatcgtcc ggtcagggct tccccccctc cgtcccgacc
     2221 ggaccttcgc accaacaggc cggagctttc gggctccggc ctctctaggt ctattccaga
     2281 cgcaaccatg ccgaaaccac actggtctga ggtgttcaag atatgccgtt cggcagacgc
     2341 gccggcctgg ggtgccacgt acactagggt cgagggcggg cgtgtcccct ccccttggag
     2401 tccgcgcccg ctctgccctg gccgcgctac tactctcatt cgcagcagtg ccttcacttt
     2461 gctccggtcc tctgcgttgg cgttttttac gtacgccaag atcacaagcc agccaatatg
     2521 tacataggta ggctaattcc cagcggggtg tgactacccc gctggcgatc ctggtcccga
     2581 gtgcgtagat cgccggtggt acctggtccg tctggaccag cccgacgcag gttgtctcct
     2641 acaggtccag taagttttcg ctaacgtctt cattacggca acgcactatt atcaccttgg
     2701 cgggcttctc tctaccatca gtctatgagg tagtttctac ttcgggaatt actccggtat
     2761 tgtgatgcgt gttacgataa gatcctggca cctgagacgt acctacgaag ggagagctgg
     2821 tggccacgct atattgtccg tgcgggcagg cacacgaact gtggggagag ttctggtgga
     2881 ccgaagggcg ctaccaatgg gtcttcttcg attacgagga aacgagcgaa acccatgctg
     2941 agcaggtcga gaactgtccc gcgtgtggta ggcggcttga gcgcaagcat ttgctctcct
     3001 gactctctgt aacaccgccc cggttccaat gaccatccat catgctacgg gattggagaa
     3061 ttatgcgcgt ttccgagtaa gaagcctctt gatatctgcg ctcttggcct atccgtccgc
     3121 gctgcgccat ttggccaacg cttcccggac gaacgaacct ttgactatcg agtagtccag
     3181 cgcatcagtc gttccacctt ctatgatatc tctctttcgc tggacgtatg cctccatcat
     3241 ctcagggttg gcgcggaggc ggtcgcggaa ggcgcgaagg tcgatcacac cctttcccac
     3301 gcacccagga atggccgtgc ttccgacatg gtccacagta aagtctggga gttgatgatt
     3361 gatcatcccc gcgaccttgc gggctacttc ctgagcgcgt ggaacatagg gcaggcacgc
     3421 tgctggagcc tgctcttgct ctcctatagg acgtccccag acatgacacc tcctcgcatc
     3481 tactcccgtc cctcgcctca gcctacacct ggcctccccc cgctccgagc ggcgctcagg
     3541 tgccgcgcgg gtgctgtagg gcgtcggtca gcagacggat gtcttcgtcc accccttcaa
     3601 gggcccgatc cacatggagg gtctccaggc gcgcccgcat gtcgtggccc tctgggtagg
     3661 agcccatgag ccgggccaga ccttcgagtc tgtcgcgaac caactccgcc ttacgcagct
     3721 cctcggccat catcgttatc cgctcgttcg ccgccctgaa ctcggcatca tctcgttcca
     3781 ccttcacctc ccgttcggct caggaacatt ctacgccacc accggcgcta ggttattggg
     3841 acgcccacaa gcccttgccc ggccctcatg cgatcttctt ctcccagccg atctagttcc
     3901 cctaccgact cgtccggcat cccccaatgc cctgcgaagc tttccttcgc ggcgctccct
     3961 gagttgcctg cgctccatcc gcatgaaatc ttcagtcacg cggaagaacc ctcctgaaag
     4021 gcgctaagtc ctaactgtgc gtatgtctct ttctcaggtg cccctgtact gtccgacggc
     4081 gaaccatacg gctgccccta actgcgttgc actaacgagt ggcgtgtagc cagggttctt
     4141 ggctagcttc tgcgaccttt accttgccgg ggttccacta cgttcgccag gaaagaaccc
     4201 aaagaatagc cacctggcag aggaaagata cgccatatag cagatgcata cccccctcgt
     4261 atccgctcat acttgccctt gttaggtaca aggtgggtct aagacaaggc cctcccctct
     4321 acagaagaag gggtaggagg agatagtgag cacagagagg accaccatca gggctcgagc
     4381 aaggagcgat ggactgcgac tactgaggac catcaaccaa acccaggctc acggggaaga
     4441 aggggcacgg gcggacccca ccagagcggc gcaccaagcg ggcctggatg tcggctccga
     4501 gcgttaccaa gatgccatgg cgtacctcat cgagcaggcc gccttgctgg gagacgcgca
     4561 catggccttc ggcgacgatg ttggagacca acacccccac ggttacgcct cctacttctt
     4621 caccaggcgg gcgctgacgc tgctggagag atgatagccc ggcctgattc acccgagtgc
     4681 ttcatcttgc ggccctggct ggtcaggggg cccgaatagg ccacgtcccg cggaccgtgt
     4741 ccccaatacc ttgctcccat tctcccaagc agcaaacgga cagaggtcta gccgccccgg
     4801 ttctttgcct tggcctaact cacggaagtg cctagaagcg agcttttcag aagataaaca
     4861 tccttcggtt tgtggggtca agaaaccgcc atgaccattc tcggctcagc ggggagtcta
     4921 ctcttgatgc tgcgtgccgc acattccatg tgcgacaata gttccccctg ctcttggagg
     4981 tagtgcaata gatccaatgc aaccatccgg attcggcgag tcatccgggc cgacccacga
     5041 aattctccgc gagagcgagg agcgcttccg gctactggtg gagggtgtca aggactacgc
     5101 catcttcatg ctcgacgcag atgggtacat cacaacgtgg aaccttgggg ctcaacgcat
     5161 caagggctac gagtccgagg agatcatcgg tgaacacttc tcgatcttct acaccgatga
     5221 ggacgtcgag cgcggacatc cggaagagga gttgcgcatc gcggccgccg atggcagcta
     5281 cgaagaagag ggaataaggg ttcgcaagga cggttcgcat ttctgggcta gcgtgttgat
     5341 cacagcgttg aggaacgagg agggtcacct ccgcggcttc gccaaggtga ccagggatat
     5401 aaccgcgcgc aaggtggccg aggaacgcga acgtttgctg acccgcgagc cagcggccct
     5461 tgagagggcc acggatatcc tggaaagcat cagcgacgca ttcttcgccg tcgatgagga
     5521 gtgtcgcttc acctacgtca acggcaaggc ggagcagttc tgggggcggt ctcgggatga
     5581 gttgctgggc aagaacatct gggaggagtt cccccaggcg gtgggctccg agtactaccg
     5641 gcagatacag cgggcaatgg aggaggacgt caccacggaa ttcgaggcta catttccagt
     5701 tctcggagta tgggtagcgg ggcgggccta cccttctcgg gaagggctat cggtgtactt
     5761 ccaagacgtt accgaacgca agctggcgga ggagtccctc agacgggtga ccgaagccga
     5821 acgcaagcgg atagcgcggg acctgcacga cagcgtccta caagatttct cctacaccac
     5881 agctgctcta ggtatgatgg tgctccaggc caaggacgcg acgtcgagga agcagctgca
     5941 ggcagcggtc gacgcagtcc gacgtggcgc tcagggcctg cgcgaggtgg tcaacaacct
     6001 gcgcctcgaa gatgaagaag atcggccctt ccccgagata gtcggggctc tggtgaggcg
     6061 gagccggacg atggccccgg cgctcgagat aagcctggag gtgggagaac acgttccccc
     6121 ggcgccgttg ggagagaccg gaacgcaagt ttcgcgcata atccaggagg cgctcaccaa
     6181 cgcccgtaag cactcggagg ccatgagggt ttctgtgagc gtcaagatgg ataggggcga
     6241 tcttcacgtc gaggtctcag acgacgggag ggggtttggt tcggagagct cgcccggcgt
     6301 gggcctgagc agcatgcgcg aacgagctgc gatcatcggc agagaactgg agatcgagag
     6361 cgaagcggag cagggcacaa gggttcggtt gcgggcccct ttgccacggg gggtcttgga
     6421 atgagtgggg aagatgggac aaagcaaata cgtgtcatgc ttgtggagga tcatcccgac
     6481 ttcagagatc tcatggaggt cctgctgggc agccagtcgg acatcaacct ggtagcgcag
     6541 gctggatcgc tcgccgaggc ccgcaatcat gccgtgaggt tcgatttcga cgtggcggtc
     6601 ctggatctgg ggctgcccga cggggatggg gtagatctga tcgccgatct gcgacaggcc
     6661 aaccccgacg tgggggtgct gatcttgagc gcgagcctcg acgctgcgag cttcgagaga
     6721 gccaccgagg ccgacgagat aatggacaaa ctagcccctc tggatgaggt actaggtacg
     6781 ctaaggcgcc tggggaacgc gtgagctcct acgaaacgtt cctgcgtggt aagtaggatc
     6841 gccaccctcc aggcgaacgg agaaggctat gccccctcac taggccctcg tcaattcaga
     6901 tcgtggcatg cgcccctcgg aaagccgagc gcggaaacgc agttcggggc tgtatgtact
     6961 cgttaggata cataactgtc tggcagcagg tatctggtga aggtccagct gctaaccgcg
     7021 cgaaaggtga tttctctgat cttcgcctgt gacgcgctct cggaagttcg gggtttcatg
     7081 tagactgcct ttgacttggt tgagagctag gggaggctcc gatgcgacgg gttcgatacg
     7141 tggttgcgat gagcttggac ggctacatcg cgggccccaa tggcgaagcg gactggatca
     7201 tcatggaccc ggacatcgac ttcggagcac tcttcgagca gttcgacaca tttctcctgg
     7261 ggcgccgaac gtttgaaggc atggcaggca cagggggtgg ctcgcagtcg gggatacaga
     7321 cgatcgtctt ctcacggacg ctgcgccaag aggactatcc gaacgtcagc atagtttctg
     7381 agaacccaga gcacgcgctg accgatctcc ggtcgaagcc gggcaaggat atatggctat
     7441 ttggtggcgg ctcactcttt cgcagcctcc tggaagctca gctagtagat gctatcgaag
     7501 tctctgtgat ccccgtgcta ctgggcgagg gcataccact gttgccgcct aatccttcat
     7561 ctgagcggtt cgagctgaag ttgactagca gtcggacgtt caagacgggg atcgtgtcgc
     7621 tggagtacga tgtggcgtac ggactagcct aacccctagc ctgttcaccc gagtgcgtgg
     7681 gaggaggatt cccagaaggt cacacaaggc aggcgcttgg tagctcgcga tcttccacgg
     7741 cataggggct cattcccggc ccacttgatc gcccgccggg cattgctcta tgatccatgc
     7801 agggtaggtg atctcagacc attcggggga agcccgatgg ataacgacac agtgttggta
     7861 cggttgggcg gactgtgcgg aatactgttc gtgctcctac tggtccccgg cgtcttcgcc
     7921 gcgcgcccag acgtgcccga tgctagcttg agtgcccctc aaatgctcga ctacttcgac
     7981 ggcaagcagg gtgcgctgct ggtagccaac gggctgtcgt ttatcttcgc cactttcttc
     8041 ttcgtatggt tcttaggcgt gctgcacgcc gtgttgcgga gtgctgaggg tgagcaaagc
     8101 ggtctgtcgt ccgccgcgct ggccggggga ctgatgttcg tggcgctcga gtcggcgggt
     8161 gcgtcggtcg agatcgtgca ccctgcgagc acatcccgct tcgagaactt tcagccggac
     8221 gctcagctcg tattcctctc acaggcgctc tccgggtggc tctatagctt cgcctgggtc
     8281 ggcttggcgg ttctgctcag cgcgacctct ctagttgcgc tcaggacggg atttctaccg
     8341 aggtggctgg cttgggctgg tttcctggcc gcattggtgg cggtgctcaa attcctccta
     8401 ccgtatgcga cattggcgct gctctggaca ttggctgtct ccgtgctcat gatcatcggt
     8461 tccgtcagct cttcaggcgt gggcacccgc cgattgggac gcgcttcgtg acgctgggag
     8521 ggatcacagc tttacacgct caagagcgta aaggatacgt ttcgcggact tcgccttgac
     8581 ggagtcttag gaggttcgct ctaagggttg agctgctcct ggatcaccga ttcgactggc
     8641 tgaggggtgg ttatggagtc tgcatgccga tgcactatct tccactcacc gtcctcgggt
     8701 ctaaagatca tcgtgaccct aagagtgaag ggggtaatgt cctgccttcc gccgagcctg
     8761 gcctgctgcc gctctatcca cacagtgtag gcaagctcag cagtcacgta cttcactatc
     8821 acctcgaagc tagtgatctc accgtctctg agctgcgcca tgctccatgg tctcagaaac
     8881 ctgctcccac ccccgcgcgg gagtgcctac caagggttag cgagggtaac gtcttctcta
     8941 tgggaccata ccttattgac aggctcgggg tttcatttca tgaactcgtc cagtgctagt
     9001 tgataccgct tgatcagttg atcgacatca tctaaggcat ccatctagag atcacctcca
     9061 cggtttacac atcttcaatc gccaccctgc gatcatccta cgttattcac ccgagtgagt
     9121 ggaaggtacg ttctcagaag ttcgtagacg cggtctacga tgttcgcgca ccaacgagtg
     9181 tccccggcgc aggcgacaac cgtgttcggc agctcgcacc ccttgacagt ctcgccgtcc
     9241 agggcgttgt tcacgacgcg gatgttagag aggtttggtc tgtccgaacg tccggagtcg
     9301 gaggcggtga tcgcaacgtc gtgggaattt ggcaggtacg cgacgccccc tatggtcgca
     9361 gcgccaaagt ggttgttccg tatggtcgca tcctgggcat cacgcacgga gatgccaccc
     9421 cggttggcat ggagggaatt gtcgtgtacc tcgttgttct cgatgagcgc ctcgcccgcg
     9481 gtgggcacgc cgcctacctc ctcgaagcgt atgccggccc gaccgttgtt gaccacgagg
     9541 ttgtcgtgta cccagaaacc gttagggtgg ctgtccgagt cgtggcagaa gttgtcgcac
     9601 cacaggccgt tgccttgcgt atcgtgaacg taggagttct tgacctcgac ctcgttacgc
     9661 gccttcatac ccgcagcgat aaaccctagg gctttcgggt cacgggtatt gttggtcaac
     9721 tcgacgcgtt cgaaggtccc cttggcactg agaataccag ccgcatcgtt gtccataacc
     9781 tcgatgccgt aatgggagct agtatccgtc atcttgccct ctgctatggc agccccgctg
     9841 ccggaagagc ctgtaaagtt accgcccgtg atcttgatgc cttcgaagtg gacggtgacc
     9901 atactgccgc cttggcctgt gggcttgaac acgttctcca cgctgtcatt gcccgcaacg
     9961 gtgcacctgg tcgtgggatc aaacgccggg ccacgctgct caaacgtcgg tgccaccgcg
    10021 cacgctacct cgtcgccatc gcttggcact atcgtcgctg acgcaacaaa ggtacagtct
    10081 gcgccgagca caaagccagt ggctgtacca ttcggatcgg cgttgatcgt agcgtcgatg
    10141 tcttggccac acggtatgtt cacaacacgc gcttgcgcat ccgcaggaga gtagaagacg
    10201 aggatgagag gcagcatgat cgccgtcgcc accaacccac gttgcagtac tttctttacg
    10261 gtggtcaagt gagcccttcc tccttgggac aggtcactac caacaacaca tgagcttcac
    10321 gacgagctac ctcgcgtgca cccttctccg gtcgaggacg ggccctcggc ccccacccct
    10381 cccattgcga gcagaagcgt atactcgaac agagcttctg gcaaatcccc ttagcatgca
    10441 tcgcccgagt gatagaaggt ggactctcgg atgtttgcgc agcctcggtc cgatgggctt
    10501 ggtttacggt agcggaccgg ggcgttgcgg gttttgagat actggccgcc ttctctctcc
    10561 cgatcaccgc tcccacttct cgaactttcc attttctcgt caatcaggaa cgatggtgtc
    10621 cggttttgcc ggtactatcg aagccattag ccaactactt ttggtggcaa gaagggggtg
    10681 gccgagcggt gatcggtttc atgagcgttg agatacaggc tgaggcggac ttcagccgtg
    10741 cccggcgtcg ggccttcttc gggcgcatag cggcccgttt gcggcgtgaa tgcagttggc
    10801 tgctggcctt cgacaagata cacctgacgc acaaccggcg tggtttgggc ctgagggacg
    10861 tcgaggtatc gaagatagtc ggcagcgtgg gccgccacga ggccttcgat cgcgacttca
    10921 tgcccacgaa ggcgagcctt gcggagaggt ggatgatggt ggaccgagcg ttccaccgcg
    10981 ggctagacct cccagcggtc aggctgtaca aggtcgggaa ctcgtacttc gtcgaggacg
    11041 gcaaccaccg cgtcagcgtg gcccgctatc aaggggtgga gacgatcgag gcggatgtga
    11101 tcgagttctt cccgctatat ggtgcacact agaggcggac agcgtaggca agcagcaaac
    11161 gaagtctatg gattcaatca tcctgttgtc cactgcttct acgttcagac cccttccacc
    11221 cgactaaaag gtcatctagt gagcctctat gcgtctgagt gaaggctact caccagatct
    11281 cgtcgtagag gtgttctgcg aaggccgcta cgagtaatag tattatcctt cctcagtcca
    11341 acctcgaatc tgagctttgc cccggtctcc taccccatcc ctcgggataa tcacgttgta
    11401 gcacccttcg cgcaagctgg tttctcagcg cgtggaagtg ctcaccgaag tccatctcat
    11461 cgtgcatcca cgcctggatt cctcgaactc ttgggaaccc acccctctgg tacgcttcct
    11521 cggagatctc ttcaaagagc tcctcagcga tgccgaggtg gtaagccgcc cgagcctcct
    11581 tctcctcaaa cccgtagagg cgcatcaagt cttctaccag cttgtggtct tcctccacga
    11641 catcttcctc ctttggcctc cgcttcctac gtatgatcta gctgccttat ccttggttgc
    11701 gcccatgatg ccaccctaag aagctcggcg cctccgacga aggatcattc tcccgagtgc
    11761 gtgtaatgtg tttgctccga tcagacccgc agcccatagc gcgaagggca caagccgttg
    11821 cgctccgcca ccaataaggc cggcacgtca gcttgacaaa cagtgccttc tcataggagt
    11881 ccacatcaag catcgtctcg gtccgacacc tatgggcatc cccagctcgt tgaggtcagc
    11941 ggcaggcttc cgtcgacggt attattcctt tctgtgggag caggacggat ggaggaaagg
    12001 aagatgaaga tcggttcgat cgtcgtccgg tgctacgagt tcgacaaggt gctagcgttc
    12061 tggcgggaag cgctccatta cgtaccaagg gagcccgcgc aggacggttg ggtggtcctt
    12121 catgatcccg agggaagagg tcccaacgtg tctctggaga aggttcctga acctttggaa
    12181 cacatcggga agaccaacag ggtgcacctg gacctctaca cgaccgacca ggagggcgag
    12241 gtggagaggc tggttgggat gggggcgagg cggtacccgc aagaatatcg accggatgac
    12301 gactttatcg tgctggaaga tcccgacggc aaccgcttct gcgtcgttca ggtccccgac
    12361 gggggttaga cgagccatga ggccccttgg ggctttaccc ttggtagcat gcgagacact
    12421 cactgtactc tgatgaagga ggagagcatg gcggagtacc tgccggaggt ctaccagcac
    12481 ttcgagcgtc gctaccctac ggtgaaggaa gcgttcgacg ccttgggctc tgccgagcac
    12541 gacgcagggc ctttaggcga gagggaacgc cgcctgatca aactcgctat cgccgttggc
    12601 gcacagtcag aaggcggcgt gcgttcgcat gtgcgcaagc tgctcggtgt cggggtctct
    12661 gaggcggaga tccttcatac gatcgtcctc gccatcacca ccatcggttt ccctgctacc
    12721 aacgcagccc tgagctgggc cgaggaagtg ttgtccgaag aaggttgacg aaggcctaca
    12781 ggtattcgcc tgtagagcgg cacactccgg tgaacggagg cgaactcgaa atagcgtgga
    12841 taagcggatc tctagcccca actgactgcg gccctttcgc aattgtcgga aggtcgcacc
    12901 aagtacgtgc gactggcatc atgaagacgc cggacaagcg tcgagcgagg aggacacctt
    12961 ttatgaacat cttccccacc aaggtccttg tggccaccga cggttctagg gaggcacacc
    13021 tggccacccg aaccgcggct gaactggccg ctaagaccgg ctcggagttg cacgtggttc
    13081 acgtcttcgg catcacccca tggtaccctg cctaccccga agccacagcc ttcaatggag
    13141 cagagttgga ggacccggtg ctggaggaag acctccagcg gatcaccgag caacgagcca
    13201 gggagctgct cgacgcagaa gtggagaagc tacgctcggt aggcgggaca ccagcgcagg
    13261 cgcacctggt ggagggaggg gtgccgcagg agatcgttga gctggccgag gagttagggg
    13321 ccgggctaat cgtgatgggc agcaggggac acggcggtat ccgaagagcc ttgatgggga
    13381 gcgtctccga ttcagtggtg cgccacgccc actgtcctgt gctggtagtg cgaaacggtg
    13441 aacgcgagag agactacctc ccgggcagga tcctgctggc cgttgatggc tctgaagagg
    13501 catcggccgc cgcccggacg gcagtggaac ttgccgaccg tatggactcg gagcttcacg
    13561 tggtgtacgt aggggaggtt aggcctgtct accaccctga aaggcgtggg tacctcgccc
    13621 gatacgaaga gatccaagaa gaagcccggc agctgctcga agagcaggtg gacgaggtca
    13681 agtcggctgg gggaaccgtc acgagagccc atctgagatt ggggcatccc gacgaggaga
    13741 tcgtcggatt gggtgaggag ataggagcgg atcttatcgt taccggcagc cggggcttgg
    13801 gtggcatgag gagggttttg atgggcagcg cctctgactc ggtggtcagg cacgctcact
    13861 gcccggtcat ggtggtacga ccagagaagg aacagactgt atagagacgt tgttcgccga
    13921 gctgcatgga aggtagatga tgcgaacttc gccttgacgg agttctagga agttgcgctg
    13981 tctcttactc tctgactatt gtgaccgggc tgtgagcgtg cctgaccacc gagtcagaga
    14041 cactgcccat caaaaccctc cttatctttc ctcgccccct gctgcccatc actatcatcc
    14101 cgatgcccag gctgtgggcc aagtcgagga tctcttcgtc tgcccgcccc agcctgaggt
    14161 gcgcctcctt gacggtgcct cctgcctcct cgactgttct cacctgttca tcaagcagcc
    14221 gctgggcctc gcgttcgagt tcagcgggtt ccacctcggt ttgagctaga aaggtcggct
    14281 caaactgtcc tacgtgaaca acatgcaact cagagtttgt ggtgttggcc agatcggcgg
    14341 cggtacttgc ggccaactga gcttccctgg agccgtcggt ggccacaagg atctttgtgg
    14401 ggaagatact cactgggcta acctcctcgc tctacgccct ttacttggca tcatgatgcc
    14461 acgatcagag gttcggcgct tgcgtccaga gtttattcgc catagaacct agaatgtaga
    14521 ttctcggacc ttcgcctgta cgttctcgga acttcgcctt gatcgctgct caggccgcat
    14581 tagaaaagag cgaagtagaa ttcgctgcgg tcatggtcgc tgccttattg tgcgcttcct
    14641 tcggagtctg gcggctaacg aaagaagtct aaccataccc tattcaccga agtgcaagaa
    14701 ggttcatttt ccgaggttcg gcgaaaggcg taaaatggtc gcaggttgct agctcgacca
    14761 ttcaataagc gcctcttgtc cgatgcccga ccattggggg ccatggccgc gcacgtgcta
    14821 gcgctggtcg ttgtcgtctc tcagcccgtt gcatctttgg cctggctcgg tgggatgcac
    14881 gccaccctgg agcaggccgc ccttcatcaa gcggccgtcg cccacgggca caaccaccat
    14941 cacggcgcat tggagcacca cgaacaccag cccgagcaca gagacgacaa gacggatgtc
    15001 cacttctccc gatccatact cgggccggag ttcacctccg cggcaccata cgccgggcct
    15061 tttcaaggcc ttctccagac tctcctccag gctatgctgg ccatgttacc agccacgccc
    15121 ttacccggcg aacagtcgcg ccatacttct ctggcggaga gtatttcgtc tcagcactcg
    15181 ccctctgtcc cgcacaggcc cccaatcctc cccctgatcc tcacaatcgc gtaacattag
    15241 aagccccact tcagccaggc ggcgatcctg tccgctaggt cgcgcgaggg cgcgtcagga
    15301 ggagtagatg aaacgaccgt cgtttgtcgt gggcggcata ctcggcctca gcgcgatgct
    15361 cgccgtatcc gccccggcct gggctcacgt ggtcgtctcc ccggaggtgg tgacagccgg
    15421 cgactacgaa acccttactg tctccgttcc gacagagaag gagataccga ccaccaagat
    15481 ccgggtagtg gtgcccgagg gcttcttgct ctccggggtc cagcccgtgc ccggatggga
    15541 gcacaccttc gaggaggatg gcggtgttgt gagggccgtg accttcactg gcggagagat
    15601 acgcccgcgg gagttccagc agttcctctt gcaggctcaa gtccccgagg aaccaggcga
    15661 atacccgtgg aaggcgatcc agacctatga ggacggcagc gtcgtagagt ggaccggacc
    15721 ccccgattcc gaggagcccg cctctgtgat cgaggtggta tccggcggat ctgatcatcc
    15781 ccaggccagc ccgcagccct cggaagcgag cgcctcccag aaagcggcgg gtggcgaaac
    15841 gaaggtcctt gcagacacgg gcgggacgaa tccggccctc tatgcaggac ttggcttcgt
    15901 tggactggtg gtttcggccc tgctagcgcg gcgcctacta gggtagagat ggcacccagg
    15961 tcgtctgtgg gacggctcgc catcgtctcg tggctggccg ccacctggct cctgctggct
    16021 tgcgcaccag cgctcgccca cgcgaacctc gtgggggcct ctcctcggcc ggggggcgaa
    16081 gtgtccaagc cccccgagcg ggtcgaattg cggttcagcg agcccgtgga cgcagagttc
    16141 gacccggtgg tcgtgcgcac cgtcagcggt gcccgagttg acgctcacaa tgcccacgtg
    16201 gatccggagg acgcgagggt cgtgctggcg gacctcgaga gggtgcccga gggatcctac
    16261 accgtcaagt ggcgtgtcac ctccatagac gggcacgtcg tggatggacg atacgccttc
    16321 gccgtggcgg ccgcgggaga ggaccgaccg tctgaagatg ctggggacag agctaccacc
    16381 gccgaagcag caggggcgca cagtggacac catggcgggc accacggagg aggtgctggg
    16441 agagtagcgt ccaccgaccg gcgagatccc gtccctgagc cagccgcggg acgcgctgca
    16501 aggatcgaac acgggctcgc cctcgccgcg accgcgttcc tcgccggcct ggcgccgttc
    16561 gcggtcctcg tgtggcttcc ggccagcagg cagtcgggcg tcggccgcgc cgcgatccgc
    16621 tctttcgggg tgctggcagg gggcctgctc ctagcgctcg ccctggcggg cgtgggcgaa
    16681 ctgtcctcct acgcggtgcg cgcctccggg gaccccctaa gcaccgagct attctggcaa
    16741 acgctcttcg acagcagagt cggcgcactc tggctgacca gactgagttt ggcgctgctt
    16801 gcggccgcag tgatcgccac ggccgcgagg tcggggcgca cctggccgtg gtgggcggcg
    16861 atcggagcga gtggcatgct tatgataacg ctgaccgggc tcagccacgc cgccgccacg
    16921 ggccgccttt tgcccctcct ggcggactgg acccacgccg tggccgcgac ggtatggatg
    16981 ggcggcttgc tgggcttcgc cgtcgccctg tgttccagag cgtttcggga cctagctcct
    17041 gacagccgga tgaagctgcg cgaccgtgcg gtgcgccgct tctcggcggt ggccaaatgc
    17101 gccgtggtag tgcctgcctg cacgagcctt tacgcggccg tcctccacat tccaaacccc
    17161 caagctttgc tcgccacgcc ctacggtcgt gccctcatag cgaagctagc cctgctgagc
    17221 ttgctgctgg ccgcaggcgc gtcgaacctc ctgctgcgcg gccgcgggcc gttcggtcgc
    17281 ctagtcgtcc tcgagctgct cctggcgctc gcgttgttcg tggccacggg cttcctgacc
    17341 agcctaccgc ccgcaagcgg cgcttagtgt gcgcaggact ggcctttctg gaggcgctat
    17401 tcatcctgcc gaggttctcg gacgtcgctt gttaagaagc ggtggtttct cctatgcgcc
    17461 tcctcccgag agtgcaccct ccgaaggcga gtagaggtgg cgcagaaacc cttcggccat
    17521 ctggcttctg accgagttct gggtgcacag aaacaacacc ttctgcttcc gcatctctat
    17581 cctccgttca ccagctccgc ttgcactcga tccttgatcc tgtctcttac cgaacggaag
    17641 accgccaact gcccctcctc cgaccccctc gcccgtgatg ggtcctcgaa ggaccagtgc
    17701 agtctgttgg cggctccagg gaagaatggg cacgcctcgt taacgtcgtc gcagaccgtg
    17761 atcacgtagt cgaaggactc ttccagatac cgctccagcg tcttcgactc ctgcccggag
    17821 atatctatcc cgatctcatc catcgcccgg attgcaagcg gccttacgtg ggtagcctcc
    17881 gtgcctgcgc tgtgcacctc gaagcgctct ccgcccagat ggcgtagcat accttcggcc
    17941 atctggctcc tcgcagagtt gtgggtgcac aggaacagga ccctggcccg ttcccctaac
    18001 tcagcagcca ccaacgctcc gcttctcggg accgggaagc aagcggtccg cggcctcgcc
    18061 gagcagccgc ccggcggcct cccggtcgag ggtgtagaag ctccacttcc cgcgccgctc
    18121 ctcgcgcacc agccctgcgt ccttgagctt cttcatgtgg taggagacct tcgactggcc
    18181 catgccaaac tggtcctcga actcgcagac gcagatgccc tcgtcctcgg cttcgaccgg
    18241 gacgcccaga tcgggcaggt cgcagcaact cctaccctcg gccatcatgc tgagcatcct
    18301 cacccttatg ggatcggaga gagcactccc cagggcgacg agccggtcta accctgcctc
    18361 ggggccgtcc ctacggacac cgtacttttc tacgaccccg agcactctag tgttcacagc
    18421 gtcctcccgg ccggcttccg acccctgatg aaggcgctcg ccagcgggac ctccgtcgca
    18481 gctgcggtcg ccactgtggt atcgtcgcag cagcccgagc cgccacagca ctcggacgtg
    18541 gccgcgaagt cctcttcgta ggtatgggtg acttccaccg agacgtcctc gaagcccgct
    18601 tctccaagca tcttctcgta ctcgtccttc tccagagcgc cggagacgca gccggaccac
    18661 gcctccatgc tcctcaccaa ctcattcgga agcttgctct tgtcgccaag gaacaccatg
    18721 tccgagacgg cgaagcgacc ccccggcttc agtacgcgga acgcctcgga gatcaccctg
    18781 ggcttgtcag cggagaggtt gatgacgcag ttggagataa ctacgtccac gtggtcctcg
    18841 ggcagcggga tatcctcgat gtggcccttg aggaactgga cgttctcgac cccagcctcg
    18901 gccctgttct tctcggccag ctcgagcatc tcgtcggtca tgtccaggcc gtaggccttg
    18961 ccgcccgggg agaccctcct ggccgagagc agcacatcta tgccgccacc gctgccgagg
    19021 tccaagacca cctcccccgc agagagcgtg gcaagggatg tggggttgcc gcaccccaag
    19081 gacgccgcac ccgccgcctg gggcagcccc tcaagctcct cggccgagta gccgccccca
    19141 gtccagtcca ccccgaaggc ctccgagtcg agacctcgct cgcccgccga tccgcaacac
    19201 gaagacgacc cagctccccc caggacagac ctcgcggccc ccgcgtacct ctccttgacg
    19261 cggtcgcgca actgttcctc catgatgctc cttccgatca gctttacttg atcggagtat
    19321 gaaccaatat atgttgatct gtcaaggccg ggagagcact atgcaagaag ttcgtcgcag
    19381 agggggtctc taaaggccgg ttcagcagtg gaagatgcgc atcgctgaga cgatcaagac
    19441 taacgacaac cttcacctca cagatcaagg gatagccgca cgggaggcgt tgatggagga
    19501 gattgaagcg atcgagcaga acctagaggc gatcaagcaa gaacacgggc tagaccaggc
    19561 aagcaccata gaacgggccg gggtatcctt tgggggtgtc caggacccag tctttgtcga
    19621 gcggggaggt taacagggtg gaagacaaaa accgcatgaa agtcaccata acggaagcgg
    19681 ccgagtatct gggcctcact cgtacgacgg ttcaggatat ggtggaacgc ggggtgctca
    19741 aggccgacaa gttcgccggc gcggtccaca tccctcggga ggaggtggag cgcatcgagc
    19801 gcgagactgc cccgtaaaag acggaccttg gctttgcgtg agatcgtcgg cggcgcgacc
    19861 atacctaccc cggcggggcg agcatactcc ggaacgcatg cctgcaactc cccccttgcg
    19921 ccctaccggc ttctatataa ggtcggggct tccacactac ggggccccga ccctttgctt
    19981 tgccccagac ctatgccgcg aggccagcaa accgccgttt cactgtacct ggctggctct
    20041 gccccgtccg aaggggttta cgtgggtgca tagtctagag ccgaatttct aatcctcttc
    20101 caacagagca aggtcgtgtg tcaatccggt tgtctctgcg tcgaagaaaa tggagtaccc
    20161 gcaattggtg catgcggcgt agttgaattg aggcgctggg gatacggcgg gaaacgcacg
    20221 agttggtgct acgctgcctc ttggctcgaa gctgtttgcc acaccacata ttgggcagga
    20281 aagaggtccg gcttgcttgc gttctaggta gcctgagaac cgttcgtcca tagttacccc
    20341 tctttgtcga gatccctgag gatcattcta actgatctag cgaggtcggc tgaaggcgct
    20401 ctgagcgtcg cgcgacccaa gcgctgcctc ctcaagacac cgtctccctt gatcttcacg
    20461 ccgactaccg ccccccgaaa accacggtat agacggtcga aggatcatca tagcccaggt
    20521 aggttcccga ccgcgcccca acaccgacct ctacaaaggc cgccctcagg atgtttgccc
    20581 tgtggccctc gctgtgcatg aactctgtga aaaggtgctc aggggtatcc tcgtccctgc
    20641 catcgcctcc catagctatg ttctcaccaa gcgcgtggta gtcgccagga tcgtaactgt
    20701 aataacctcg acgctccagc tggtctatca ccgtgacacc atccggcgtg tagtgtgaga
    20761 agtattccct gttcaacata tcttgggagc gagaacgggc agcctgagta agaacaggtg
    20821 acaggcatag ggctctacgt ccgtgcgccc gcctcgtact gttgtgcaga tcgaacatcc
    20881 tcttctctgc cacgttcagg gcgaaggtgc cgccatcaca agtcagcata ttcaccaacg
    20941 tagaagtatc ggccggcggt ctggctccgc gtatctcgga ctcgttgcgt tgtgaagctc
    21001 ccttcgtggc gcgtggggct ggtgcctcca cacctgacgg ctgggctgcg ctgggtgatt
    21061 tgactaccgg ctctttctcg ggtgccacat cgagcggctc cgcgaggcga acgcctacag
    21121 aagtatgagg ctccgagaaa cgaacgctca caggagcatg agagcctgtt gtttgccctt
    21181 gcccatcatg acgcatcgca tataccgccg cggcccccgt gaaggacagt gcaacgaaaa
    21241 caagccccac gccgatcact aacaacgcgc ccccacgcaa cctaatcaca tcccctgaag
    21301 tagcggaaat tcctcgaccc atcggcggat gtcgtagcgc ccctgctcgt cgtactcggc
    21361 cgagtagacc cgctcgcgtt ccgcttgggt gctgatcagt gcggactcca tcctctcccc
    21421 aattcgacag acctcgggac atactagcgc acgagaatgt caggcgctag cgaccgcctc
    21481 agtcgctatc agtcgcatct cagggaccaa aagggcctat ccttcacctc tttcgccaag
    21541 cgctgcgtgc acagcctcaa gcagctgata tctttctgca tctttcaaga tatagtcagt
    21601 ggctcccgca cttgcagcct cccgacggta atcctcactc tcgtatgcgc tcaccaatag
    21661 gacgctgatg gtgggcatct ctttcttgat ctcccgagtc gcagttaggc catccatctc
    21721 tggcatcctt acgtccatca gcactagatc cggacgcaac tgccggcaga gctccaatgc
    21781 atgatggccg ttagccgcct cacctaccac ctccaagtcc ggctctcctt ccagcatgag
    21841 ctgcgttgac tctcttatca aatcgtgatc gtcgacgatg agtagtcggg cccgccgcac
    21901 gccttcatcc tcgttcatgg tcgccacctc tcccttcaaa cgccgctggc aatgacacct
    21961 ctgccactat ggaagtgcca gcgcctggtt tgctcctgat ctcgagctcg ccacccagca
    22021 gtacgactct ctccctcatg gtggagaggc ccaacctctc caccggaccc tgctcagcca
    22081 atgccgctga cgggtcgaac cctcgcccct catccctgat ctcaagccgc acctttctcc
    22141 cccgctgcgt cagcgcgacg cacaccgcgg tggttcgggc gtgcttttgc acattattga
    22201 gcgcttcttg ggcgaccctg aagagcgtga tctctacctc gggcggcaac cgttctcccc
    22261 cgagggtctc ctcgtagcag atctcccaac cctcattctg tagttcctcg acacgctgtc
    22321 gtacagcgat ctccaaccca aagtcgtcga gcgttgtggg cctgagaccc ttgaccaagc
    22381 gcctgccttc ttgcaccgtc cgccgaacca gctccagtgg acggtctagt tccccatgtc
    22441 ttaccttgga gcccggggga tgagtatctg cgaaagtctg tatgtaccgt tgggcagcga
    22501 tcaccatctg gatcagactg tcgtgcatct cgtaggctag ccaacgcctt tcctcttcgt
    22561 gggctgtcat gagctt
//
LOCUS       DASTKM010000332         8723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_496452, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000332 DASTKM010000000
VERSION     DASTKM010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496452"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFQ10_13330"
     CDS             complement(<1..205)
                     /locus_tag="VFQ10_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966371.1"
                     /translation="MIARILVGVAGAIAAPVVGGLAYRKVRQRRVAKSLMIDTPNGIV
                     EERYVRISGIEQWIQIRGENRTNP"
     gene            complement(417..596)
                     /locus_tag="VFQ10_13335"
     CDS             complement(417..596)
                     /locus_tag="VFQ10_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966372.1"
                     /translation="MFYDVRLTAYYCGTTATASISTKKSGCARPETNTTVMAGGLGVS
                     GQAFWKVAKPACSGG"
     gene            complement(808..981)
                     /locus_tag="VFQ10_13340"
     CDS             complement(808..981)
                     /locus_tag="VFQ10_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966373.1"
                     /translation="MASEDERLDILVNAAGGATVGGLMDLDDERWQADIDKLLGYLRM
                     MRAASQEYPEVKE"
     gene            1116..1787
                     /locus_tag="VFQ10_13345"
     CDS             1116..1787
                     /locus_tag="VFQ10_13345"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9966374.1"
                     /translation="MWSRIRSDTSTNPRKLPTQDRSKETVEAILQATALVLKREGYDR
                     ASTRRVAEVAGVSVGSLYQYFPNKESLVVALYNRHLRELISMFESRFEESVRAPLPEA
                     IRGLVGASLRLHTVDPELHRVLVEQIPRSGRPDPEDALAGRVQEMFRAFLEEREAEIR
                     PENLDLAAFMVIEAVEAITHAAVLDRPEYLEVEDFVEEISTLVLSYLSPDGASDPERT
                     QDPRT"
     gene            1970..3556
                     /locus_tag="VFQ10_13350"
     CDS             1970..3556
                     /locus_tag="VFQ10_13350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9966375.1"
                     /translation="MEGEEVYRRRWWILGVLCLSVVIVQLGNLILIVALPTIQQDLGA
                     SPSELQWIVDAYTLIFAALLFTSGSLGDRFGRKLALTTGLGFFGLASLVTAFSVNPEM
                     LIAFRAVMGVGAALILPATLAIINNVFPPQERGKAIGIWAGMLGISIPLGPVISGILL
                     EIFWWGSVFIINVPITVVALGLGRVLVPESSDSDAPHPDIPGALLSIAGLAVVVYGVI
                     SASDEGWTDPSVLLAIGGGLLILALFVGWELWTEHPMIPMRFFRIPAFSGAAATMLLL
                     AFSMFGVAFMLTLYLQNVLGYGPLAAGLRLVPMFTVAIGAPLGASLTRRIGRKVTVPL
                     GLFIAAFGLFALSTLTISSEARILWALAIMGLGLGTAMSPTIDALLGSIPREKSGVSS
                     AAQQTSIQLGGTLGVAVLGSVLNSTYADSVEGSVSRLPIPEAARGAITDSLAAAIQVA
                     QQVGGPAGGQLAEAAREAFVEGMGVATLTAVGLMLAAAMVAAITLPNKDPKPELPSEA
                     EQQPLAREVGASKTKPTERS"
     gene            3707..4396
                     /locus_tag="VFQ10_13355"
     CDS             3707..4396
                     /locus_tag="VFQ10_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9966376.1"
                     /translation="MHVIEARELTKVYGRGENRVEALTGVNMNVEPGEWIAIMGPSGS
                     GKSTLMNLIGLLDRPTSGSYTLDGRDVFRLQDSELARARRDLIGFVFQSYNLLPRESA
                     LKNVELPMVYAGVGRKERRKRALGVLETVGLAERAHHRPPELSGGQMQRVAIARALIN
                     RPALLLADEPTGNLDTVSGAEILDLFEELNASGVTVVVVTHDAEVAARTRRVVELRDG
                     RIVAGEPSEVV"
     gene            4396..5577
                     /locus_tag="VFQ10_13360"
     CDS             4396..5577
                     /locus_tag="VFQ10_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9966377.1"
                     /translation="MGKMLRIAGMSLSGLMVNRGRSALSMLGIVIGVFAVILLTSLGN
                     GVQEAITGKLTGVGSNLLLISPGNSEAQGDGPLAARSASTLTREDVEKVRSLPTVAAA
                     SASVPTAALVGTKTIPMTGVDPSYAQIRSVDLAAGRFVEHSGEVVLETAVAKDLLKSG
                     PKDAVGKTLKIRGKSYEVVGVLEVVEPAFGPPVPETSYMITNDALALSGAGNVGQIVA
                     EARSAGSVDRAAKEIGARLKSAHGGTEDFNVETQKDLLSVFTLATDLLTILLGGIASI
                     SLLVGGIGIMNIMLVSVAEHTREIGVRKAIGATDTDVLFQFLFEALLLTLIGGLAGIG
                     LGVGVSALLPEINPIIPTSVTGTSIVLAFGVSALVGLVFGVLPAYRSARLEPVKALRR
                     E"
     gene            5591..6226
                     /locus_tag="VFQ10_13365"
     CDS             5591..6226
                     /locus_tag="VFQ10_13365"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9966378.1"
                     /translation="MELRQRFTAAVRAQFGRPTGFWGRAAGLLMAHRSSNRRRNAWAV
                     SLLDVRPEDRVLEIGFGPGIAIRELGRLAWKGYVCGIDHSELMLRRAKRLNAEGLRRG
                     VVDLRLGSVPEDLPAFDEPFDKILAVNATMFWREPVARLEELRRLLRSGGLIAVAHQP
                     RGPGASDETSAAWGQEMEAALAQAGFSEVRLETMALKPAVVCALGVNRRDA"
     gene            7049..>8723
                     /locus_tag="VFQ10_13370"
     CDS             7049..>8723
                     /locus_tag="VFQ10_13370"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9966379.1"
                     /translation="MQTTNEKERLARITIPVTGMTCASCVWRVERTLSEKEGVAEASV
                     NFAAEKASVTYDPTTTNPDDLIEAIRDAGYGADVRETTFSVTGITCASCVGRVERALE
                     KVPGVLEASVNLANERATVEYLAGEVEPRDLEMAIEGAGYGVVREEDSSVEDSHEREY
                     RKLKSDFLLAAALTALILLGSLPHMLGLMLPIPTGWLNLALLLLATPVQFWAGWRFYR
                     GAWGALKHWQANMNTLVVMGTSAAYLYSAVATLAPGLFAAGRADVYFDTSALIITLIL
                     LGRLLEARAKGRTNEAIKKLAGLQAKTARVVRGGEELDVPVESVEIGDVVVVRPGEKI
                     PVDGRVVSGESAVDESMITGESIPVMKREGDEVIGATVNGSGSFCLEATKVGQDTVLY
                     QIMRMVEEAQGSKAPIQRLADRISAVFVPAVIGVAAVTFVIWLLLGPEPALTFALLNT
                     VAVLIIACPCAMGLATPTSIMVGTGKGAESGILIRGGEALEGTHKLDTLVLDKTGTLT
                     RGTPELTDIRVEDGIREEELLRLVASAERASEHPLGEAIVRGAKDRGLPL"
BASE COUNT         1592 a         2667 c         2863 g         1601 t
ORIGIN      
        1 cggggttggt gcggttctcg ccccggatct ggatccactg ctcgattccg ctgatccgga
       61 cgtagcgttc ctcgacgatc ccgttggggg tgtcgatcat cagcgacttg gcgacccggc
      121 gctggcgcac cttccggtag gccaacccgc cgaccaccgg cgctgctatg gcacccgcca
      181 cgccgaccag aatgcgcgcg atcacgtgtc ctccttgagt ctctagaatt ccatctcttc
      241 gacccacacg tactgctagc gtatcagaaa ccggggcgag ggcgcatggg aatattcatg
      301 tcctggggaa cttcggagaa cccgaattct tttacccagg ttggtgaatg gccggttctc
      361 cgaagttcgt cgtgacctga tactcagggg gcgacagtat cgcgatctag tgcttgctag
      421 ccaccgctgc aggctggctt tgccaccttc cagaacgctt ggcccgagac gcccaacccg
      481 ccggccatca cggtggtgtt cgtctcgggt cttgcgcatc ctgacttctt ggtggagatg
      541 gacgccgtcg ctgtcgtacc gcagtagtag gctgtgagcc gaacgtcgta gaacactcct
      601 aacttgccgt tgcacgtgag ccgggcacga gcgtcttccc gctagaccag cgccacctcg
      661 ccgccgaggc tccttcggtc agcagggcgc ggaaccttag ctcagggtgt gtgtagtttc
      721 ggaactttga tgattatcat cacgccccac caatgaggca caccgaagca ctactcgata
      781 cccatcgagc gaatagaatc actacgctca ctctttcact tcagggtact cttgggaggc
      841 ggcgcgcatc atgcgcagat acccgaggag cttgtcgatg tcggcctgcc acctctcgtc
      901 atcgaggtcc atgaggccac cgacggtcgc acctcctgca gcgttgacca ggatgtccaa
      961 ccgttcatcc tcactggcca ctgactggaa aaccctccta caaagcagct cagtgaaaaa
     1021 gtctattgga ctttggcgtt gttgttttcc cctacttccg taagaggctc taaactacat
     1081 aagccgatct atcgttcgcg tcattatcgg aaagggtgtg gagccgaata cgaagcgata
     1141 cttccacaaa cccacgtaag ctgccaacac aggatcgctc gaaggagacg gtcgaggcaa
     1201 tcctgcaagc gacggccctc gtcctcaaac gagagggcta cgaccgggcg agtacaaggc
     1261 gggtcgcgga ggtcgcggga gtctctgtgg gctcgctcta ccaatacttt cccaacaagg
     1321 agtcgctagt cgtagccctc tacaatcgcc atcttcgtga gctgatctcc atgttcgagt
     1381 cgaggttcga ggaatctgtg cgggcgccct tgccagaggc tatccgagga ctagtagggg
     1441 cttcgctgcg gctgcacact gtcgacccgg aactgcaccg ggtgctggta gagcagatcc
     1501 ccaggagcgg gcggcccgat ccggaggatg ctctggcagg aagggttcag gagatgtttc
     1561 gagccttcct cgaagagcgc gaggcggaga tccgtccaga aaacctcgac ctcgctgcgt
     1621 tcatggtcat cgaggcggtc gaagccataa ctcacgccgc agtcctcgac cgcccagagt
     1681 atctcgaagt cgaggatttc gttgaggaga tctccaccct agtcctctcc tacctctcgc
     1741 ctgatggcgc cagcgatccc gaacgaacgc aggacccccg aacgtagatc acagaccccc
     1801 gcccgagcgc cccaccaaaa tacgagcgcg ccatccacaa aacttccgtt ccaccatgaa
     1861 aacggctttc cttccgcccc gaagtacccc gcagaaacgc gaatttaaaa cccgaacaat
     1921 tgttcatata atgtgtcagc ggccactgag ctgaccaagg aggtaaggcg tggaaggtga
     1981 agaggtctat cgcaggcggt ggtggatcct gggggttctg tgcctgagtg tggtgatagt
     2041 gcaactgggt aacctgatcc tcattgtggc gctgcccacc atccaacagg atctcggtgc
     2101 ctcccctagt gagttgcagt ggatagtgga cgcctacact ctgatctttg ccgccttgct
     2161 gtttaccagt gggagcctgg gggaccgctt cggacgcaag ctggcactta ccaccgggtt
     2221 ggggtttttc gggctggctt cgctggtgac agctttctcg gtcaacccgg agatgcttat
     2281 agccttccgg gcggtaatgg gggtgggcgc ggcgctcata ctgcccgcta ctctggcgat
     2341 catcaacaac gtcttccccc cgcaagagcg cggaaaggcc atcgggatat gggcagggat
     2401 gttggggatc tccatcccac tggggccggt gatcagcggg attttgctgg agatcttctg
     2461 gtggggatcg gtgttcatca tcaacgtacc tataacggtg gtggccctcg gtctcggtag
     2521 agtgctggtg cccgagtcca gcgactcgga cgctccccat cccgacatac ccggggcgtt
     2581 gctctcgata gccggtcttg ccgtggtggt ttacggggtc atctctgcct ccgatgaggg
     2641 ctggaccgac ccgagcgtcc tcctggcgat cgggggtggc ttgctgatac tcgccctctt
     2701 tgtagggtgg gagctatgga ccgaacatcc gatgatcccc atgcgatttt tccgcattcc
     2761 tgcctttagc ggagccgccg cgacgatgct gctgctggcc ttcagcatgt ttggcgtggc
     2821 cttcatgctt accctgtatc tgcaaaacgt cttgggctac ggcccgcttg cggcaggcct
     2881 caggctggtg ccgatgttta cggtcgccat cggcgctcct ctaggagcct cccttacgcg
     2941 gcggatcggg aggaaggtca ccgttccttt ggggctcttt atcgccgcct ttgggctctt
     3001 tgcgctctcc accctcacca tctccagcga ggcgaggatc ctgtgggccc tggcgatcat
     3061 ggggctgggt ttggggacgg cgatgtctcc gacgatagac gcgctcctgg ggtcgatacc
     3121 tcgggagaag tccggagtca gctcggccgc tcaacagacc tccatacaac tggggggcac
     3181 attgggggta gccgtgctgg gcagcgtcct gaactccact tacgcggact cggtggaagg
     3241 ctccgtctct cgtctcccaa taccggaggc tgccagagga gcgatcaccg actcgctggc
     3301 tgcggcgatc caggtcgcgc agcaggtcgg cggcccggcg ggagggcagc tggcggaggc
     3361 ggccagggaa gcctttgtag agggcatggg cgttgcgacc ctcaccgctg tcggattgat
     3421 gctggcggcg gcgatggtag ctgctattac cctgcccaac aaagatccca agccagagct
     3481 accctcggag gccgaacagc agcccttagc aagagaagtc ggtgccagca agacaaagcc
     3541 aacagagcgc tcgtagatga ctttggtagt aagcggtgat cggaagggca cctatggcag
     3601 cgactagacg tggcgccccg gacggcgagc aggtcgaaaa ggaccgccgc tactgggacg
     3661 aaatcatgaa ctcgaaccag ctcaagtctt ccgagaggag ttgagaatgc acgtgatcga
     3721 agccagagaa ctcacgaagg tgtacggtcg gggagagaat cgggtggagg cactgaccgg
     3781 ggtgaacatg aacgtcgagc ccggcgagtg gatcgccata atggggccgt cagggagcgg
     3841 aaagagcacc ttgatgaacc tcatcggcct tctggaccgg ccgacgtccg ggtcctacac
     3901 gctcgacggg cgcgacgtgt ttcgtctgca ggacagtgag ctggcgcgcg cccgcaggga
     3961 tctcatcggg ttcgtcttcc agagctacaa cctcctgccc agagagagcg cgctcaagaa
     4021 cgtggagctt ccgatggtct acgcaggcgt gggcaggaag gagcgacgca agcgggctct
     4081 cggggttctg gagacggtcg ggctcgccga gagggcgcac caccgcccgc ccgagctctc
     4141 cggcgggcag atgcagcggg tagctatcgc cagggcgctg ataaacaggc ccgccctgct
     4201 gctggctgac gagccaaccg gcaacctgga cacggtctcc ggcgccgaga tcctcgacct
     4261 gtttgaggaa ctgaacgcct cgggggtcac ggtcgtggtc gtcactcacg acgcggaggt
     4321 cgccgcgcgg acgcgacgag tagtggagct ccgcgacggg cggatcgtcg ccggcgagcc
     4381 atcggaggtg gtgtgatggg aaagatgtta cggatcgccg ggatgagtct ctcggggctt
     4441 atggtcaacc gggggcgtag cgccctgagc atgctcggga tcgtcatcgg ggtcttcgcc
     4501 gtcatcctgc tcacgtctct gggcaacggg gtgcaggagg cgatcaccgg caagcttacc
     4561 ggcgtcgggt ccaacctgct cctcatcagc ccgggaaact ccgaggcgca gggcgacggc
     4621 ccgttggccg cccgctccgc gagcaccctc acccgggagg acgtagagaa ggtccggagc
     4681 ctgcccaccg tcgccgcggc ctcggccagc gtccccaccg cagcgctggt tggcacaaag
     4741 accatcccga tgaccggggt ggacccctcc tacgcacaga tacgctctgt ggacctcgcg
     4801 gccgggcgct tcgtggagca ctccggtgag gtcgtcctgg agaccgcggt ggcgaaagac
     4861 ctcctgaagt ccggcccgaa agatgccgtc ggcaagaccc tgaagataag gggcaagagc
     4921 tatgaggtcg tcggcgtgct cgaggtagtg gagccggcgt tcggcccgcc agtgcccgag
     4981 acctcgtaca tgatcacgaa cgacgccctc gcgctctcgg gagccgggaa cgtggggcag
     5041 atagtcgccg aagccaggag cgcaggctcc gtggaccggg cggcgaagga gatcggggcc
     5101 aggctaaaga gcgcccacgg cggtacggag gacttcaacg tcgagaccca gaaggaccta
     5161 ctctcggtct tcaccctggc cacggacctc ctcaccatcc tcctcggtgg cattgctagc
     5221 atctccttgc tggtcggcgg catcgggatc atgaacatca tgctcgtctc tgtcgccgaa
     5281 cacaccaggg agatcggggt ccgcaaggcg atcggagcaa cggacaccga cgtgctgttt
     5341 cagtttctct tcgaggcact gctcctgacc ctgatcggtg ggctcgcggg catcggcctc
     5401 ggcgtcgggg tctcggcact gctgcccgag atcaacccga tcatccccac ctcggtgacc
     5461 ggtacgtcca tcgtgctggc cttcggggtg tcggcgctgg tggggctcgt cttcggagtc
     5521 ctgccggcct accgttcggc acggctagaa cccgtcaaag ccctgagaag agagtaaagg
     5581 agtcaagccc gtggagctga gacagaggtt caccgccgcc gtccgcgccc agttcggacg
     5641 cccaaccggc ttctgggggc gcgcagcggg cctgctcatg gcccaccgct cgtcgaaccg
     5701 caggcggaac gcgtgggcgg tgtccctgct cgacgtgcgg ccggaggatc gcgtgctgga
     5761 gatcggcttt ggaccgggca tcgcgattag ggagcttggc cgcctcgcgt ggaaggggta
     5821 cgtgtgcggg atcgaccact ccgagctgat gctgcggcgg gcgaagaggc tcaatgccga
     5881 gggcctcagg cgcggagtgg tcgacctgcg gctcggctcc gtccccgagg acctgcccgc
     5941 cttcgacgag ccgttcgaca agatcctcgc cgtcaacgcg acgatgttct ggcgtgagcc
     6001 ggtcgcgcgt ctggaggagc tccgccgtct gctgcggagc ggtggcctga tagccgtggc
     6061 ccatcagccg cggggcccgg gggccagcga cgagacctcg gcggcctggg gtcaggagat
     6121 ggaggcggcc ctggcccagg ccggcttctc cgaggtgcgg ctcgagacca tggccctgaa
     6181 gcccgcggtg gtctgcgcgc taggcgtgaa ccggagggac gcttgacggc ggagcccggt
     6241 ccccgtcgcg ggcccgtccc actggtgcgg gcgctcggcc gagcggtcaa ctcggcggtc
     6301 gcccggttcc cctggagcta gcggctcttc agcggtcccg tgcggaggtt cttcgactcg
     6361 gtcgcggtcg gctgggacga gcgcatccga tccgactcgc ccgaatactt gtattacgat
     6421 cgcgacacca ggctagtagg tataggccct gcggcaaaac gtagccctgc cgcacttaac
     6481 ctcaagaccg gccgcggggg gcgctactac cgggtgggtg taaccgggtt tatgcgtccg
     6541 cgtgtcctac catctggctg ggccgtaagg ttgcgtcgac tgctatgctc ggcgtatttc
     6601 aagaagtctg gtgcggccat accggttacg tataggactc tcgaacgtac tgcgcctctt
     6661 atgcccagct gcaaccgacc ggtccgcggt aagcgaagtg tgtaagatgg catcacgccc
     6721 gtcccttggg ctctgtatct ctccctgttt tcgtaacccg acgaacccga cgacgagcat
     6781 aaggatccgt gcgggaggac ggtctagacg catcggggct cggggtcggc agaggaatta
     6841 gggacacttc gagggtattg tcaggggtgt gtcgagatcc tgggaggccc caaagatcat
     6901 tgccttcctc tatcagaccc ctacgatagc tagctcgcgt gaatcgacta cgctgcctag
     6961 tcgctcaagg caataccatc ttgacatatg gggtacgggg gtatagcata acgttgcccc
     7021 gaactagcgt gaaggcggga agcccagcat gcagacgacc aacgagaagg aacggctcgc
     7081 gcggatcacc atccccgtca cggggatgac ctgcgcctcc tgcgtgtggc gggtagagcg
     7141 gactctctcc gaaaaggaag gtgttgccga ggcgagcgtc aacttcgccg ccgagaaggc
     7201 gagcgtgacc tacgatccca cgaccacgaa cccggatgat ttgatcgagg ccataaggga
     7261 tgcgggctac ggcgcggacg tgcgcgagac gaccttcagc gtgacgggca taacgtgcgc
     7321 gagctgcgtg gggcgggttg agagggctct cgagaaggtg cctggcgtcc tcgaggcgag
     7381 cgtgaacctc gccaacgaga gggcgacggt cgagtacctc gcgggcgaag tggagccaag
     7441 agacttagag atggccatcg agggtgcagg ctatggagtg gtgcgggagg aggactcctc
     7501 tgtcgaggac tcccacgagc gcgagtacag aaagctcaag agcgacttcc tgctcgcagc
     7561 cgcactcacg gcgctcatcc tcctcggcag cctgccgcac atgctcgggc ttatgctccc
     7621 gatccctacg gggtggctca acctcgcgct gcttctcctc gccacgccgg tgcagttctg
     7681 ggctgggtgg cgcttctacc gaggcgcctg gggggcactc aagcactggc aggccaacat
     7741 gaacacgctg gtggtcatgg gcaccagcgc cgcctacctc tacagcgcgg tggcgacgct
     7801 cgctccgggg ctcttcgcag ccggcagggc agacgtatac ttcgacacct cggcgctcat
     7861 catcaccctg atcctgttag ggcgtcttct ggaggccagg gccaagggtc gcaccaacga
     7921 ggccataaag aagctcgctg ggctgcaagc caagaccgcc cgagtcgtac gcggcggcga
     7981 ggagctcgat gtgcccgttg agagcgtgga gatcggcgac gtcgtggtcg tcaggcctgg
     8041 cgagaagatc cctgtcgacg ggcgcgtcgt ctctggcgag tcggcggtgg acgagtcaat
     8101 gatcaccggc gaatccatcc ccgtcatgaa gagggagggt gacgaagtga ttggggcgac
     8161 cgtgaacggt agcgggtcgt tctgcttgga ggcgacgaag gtcggccagg acaccgttct
     8221 ctaccagatc atgaggatgg ttgaggaggc tcaaggctcc aaggccccca tccagcgcct
     8281 agccgacagg atcagcgcgg tgttcgtgcc cgcggtgatc ggcgtggcag ccgtgacgtt
     8341 cgtgatctgg ctgctactcg ggcccgagcc ggccctcacc ttcgcgctcc tgaacactgt
     8401 agcggtcctg atcattgcct gcccgtgcgc gatgggcctg gcgacgccaa cctcgataat
     8461 ggtcggcacc ggcaagggag cagagtcggg gatcctgata aggggcggag aggccctcga
     8521 gggcacccac aagctcgaca cgctggtgct cgacaagacc ggtaccctca cgaggggcac
     8581 gcccgagctg accgacatca gggtcgagga cggtatccgc gaggaagagc tcttaaggct
     8641 cgtggcgtcg gctgagaggg ccagcgagca tcctctgggt gaggccatcg tgcgaggcgc
     8701 gaaggatcgc ggtctcccgc tcg
//
LOCUS       DASTKM010000333         7457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_144846, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000333 DASTKM010000000
VERSION     DASTKM010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144846"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="VFQ10_13375"
     CDS             complement(<1..1425)
                     /locus_tag="VFQ10_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9966380.1"
                     /translation="MKVIVARDLVKYHGGDLKVLSGATLSVEAGEKIGLVGRNGAGKT
                     TLLDLLAGHSEPDSGSVEHPGGAKVGMTTQKLYLGERGAHSLEEEIISAFEPLMAREK
                     ELEELGTRLSEDPSNALMERYGRLQSEFERYGGYEYRARAASTITSLGFAPEDWKRPV
                     GSFSGGEQSRIALARLLMEEPDLILLDEPTNHLDLRAIEWLENFVKGARSAVLVVSHD
                     RYFLDAVAGSILELEGGRLARYPGNYSKYVAEKRAREEQLARKAKANAERRAQLERFV
                     EKNRAKSSKASQAKSKQKLLFRMEKIEDPRSASKNVRVNLGGETTRAGRVVLEMEDVR
                     YAYDDADGPLLEGLDLVIERGERVALLGPNGTGKSTIMRLATGELSPQRGMVRPGNNV
                     TLVYQDQQLARLDDSKTVLREAMDATGFDAPEARDLLGAFLFSGEDVFKKVRALSGGE
                     RNRLSLAEIVVSGANLLLLDEPTNN"
     gene            1424..2221
                     /locus_tag="VFQ10_13380"
     CDS             1424..2221
                     /locus_tag="VFQ10_13380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9966381.1"
                     /translation="MAQELYRGDPLTPGEREEFEDDLIACCDRELDLLGDITRLNVLY
                     AGGSSLLWIEGLSQRIGDGGSVTAIDMDAERVDDTQASLGEAELAAPVHLLAGDVLGM
                     PFEPHAFDLVYSSGLFHELDVKERAAEDALAALHLVAQSGGRVATSDFVDSEPAVQLE
                     DERLEAQLRREAYAQEPFGIGPPQRLVALHEKLLTDVRWLVSPPRRIRHLGKVVLAEN
                     EPAELSSLPLATAKRLRERRDAMRSRILGEGYTRPATLYVEGLVQNA"
     gene            2327..3961
                     /locus_tag="VFQ10_13385"
     CDS             2327..3961
                     /locus_tag="VFQ10_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9966382.1"
                     /translation="MTLIGLSSVEKSYGGRAVLRGLGVKVNAGARIGLVGGNGSGKST
                     VLRILAGTEDVDAGEVIRRRGLSVASLPQYIQGDERTPMDVVRASRPEISDLQEELEA
                     CEEQLGLPRVAADLRRMQRVLERQDRLLRRFTELGGPGFVGEARRYLRSLGLGDGDIY
                     RPMSELSGGQRKLAVLASCLARRPDVLLLDEPEAHLDAGRRELLEAIVRTFDGAVVIV
                     SHDRYLLDETVVEIAELEDGKITSWPGNYSAYTVARELKLKRQQQLYVAQQKEIARLE
                     EAIRRFKLWASMVVNERHIKQARNKQRQIDRMDKVEHPVLERRKIGLEFRGRVRGGRK
                     VIELKDVSMAFGEDPVLIGVDLTVSRGERLGIVGPNGAGKSVLAKVLSGLLPPTEGER
                     WAGPSIDIGFLAQNDEPPPGASPLGLVRDTKPLYEDEAVKLLGRFLFCYEQVREPVTS
                     LSGGERTRLQLLLLMLREPNFLVLDEPTNHLDIDSLEILEAELERFAGTVVFVSHDRY
                     FLDRIADGIVEVSEGEVHSHEGGYSDWRRSAFQHLS"
     gene            complement(4078..4518)
                     /gene="dtd"
                     /locus_tag="VFQ10_13390"
     CDS             complement(4078..4518)
                     /gene="dtd"
                     /locus_tag="VFQ10_13390"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET9966383.1"
                     /translation="MRVVLQRVKEASVTVEGERISGIGPGLLLLVGVAQGDGEPEADW
                     LAEKIAGLRIMSDDEGKMNRSVREVGGAILAVSQFTLLADTRKGKRPSFVGAAPPEDA
                     TRLFDHFCERLRAAGVHPVETGRFGAMMDVSLVNDGPVTILLER"
     gene            complement(4524..4910)
                     /locus_tag="VFQ10_13395"
     CDS             complement(4524..4910)
                     /locus_tag="VFQ10_13395"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET9966384.1"
                     /translation="MEPTFQKLFTVEEANALLPSLREILDEAALHRDALREKAPHMEP
                     ILRAAPWNGGGKAGSEYGVEAYNLYLAIGRITELGVVLKDLDMGLLDFPHERRGRIVF
                     LCWHPPEERVEYWHDIDAGYAGRQPL"
     gene            complement(4923..5684)
                     /locus_tag="VFQ10_13400"
     CDS             complement(4923..5684)
                     /locus_tag="VFQ10_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9966385.1"
                     /translation="MKSSTQRINYAAPYMYAVISDIHGNLEALEALLGDVPEEVETIY
                     CLGDVIGYGANPDECCDVVRSLRMPTISGNHDLAVTDLETDLNWFNPTAAAAVLWTRN
                     HLSEENAKFLRTRPRMMQSEEALFVHGSVRDPDEYIINTISAYDNLAMLKSEYPDVPI
                     CYFGHTHVKAVAPSPNGAMLGGHTLDLHSGGPYLVNPGSVGQPRDGDTFASYVIADGE
                     RVTYRFVEYDIRKAQAKIRAAGLPDMLADRLAVGR"
     gene            5754..5826
                     /locus_tag="VFQ10_13405"
     tRNA            5754..5826
                     /locus_tag="VFQ10_13405"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5787..5789,aa:Arg,seq:ccg)
     gene            5987..7063
                     /locus_tag="VFQ10_13410"
     CDS             5987..7063
                     /locus_tag="VFQ10_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9966386.1"
                     /translation="MSKGKRGRGEGSIYRRKDGRWVGQYDVDGKRRYIYGKTRKEVAS
                     KLTKAMAERDAGMVFDALSLSVGNYLDRWLDSRRDTLRRRTWTRYEEIVRLHLKPSLG
                     NTKLEKVNALQIQSLYRAKLEEGLSPRTVQIIHATLYKALKQAVKWTLLPRNVVDSVD
                     PPRVPKGEIRPLSEEQVKKLLETAQGDKLEALYILAVHTGMRSGEILGLRWQDVDLQG
                     GTVQVRRSVFGGRTEAPKSANGNRSIRLTETSIRALQGHERASEWVFSTSVGTPISVH
                     NLHNRSWKPLLASAGLPHNTRFHDLRHTCATLLLTKGVHPKIVQEMLGHSSITITLDT
                     YSHVLPNMQEKAVEAMEDIFEDHK"
BASE COUNT         1458 a         2225 c         2347 g         1427 t
ORIGIN      
        1 gttgtttgta ggctcgtcga gaagcagcag gttagcaccg ctaaccacga tctcggccag
       61 gctcaaacgg ttcctctcgc cgccggagag ggctctgacc ttcttgaaga catcctcgcc
      121 ggagaacagg aaagcgccga gaaggtcgcg ggcctcggga gcgtcgaagc ccgtggcgtc
      181 catggcctcc ctcaggacgg tcttggagtc gtcgaggcgg gccaactgct ggtcctggta
      241 gacgagcgtt acgttgttgc ctggcctgac catgcccctc tggggggaga gttcacccgt
      301 ggcgaggcgc atgatcgtgc tcttcccagt cccattggga ccgagcaggg ccaccctctc
      361 cccgcgctcg ataacgaggt cgaggccttc cagcagcggc ccgtcagcgt catcgtaggc
      421 gtagcgcacg tcctccatct ccagcacgac gcgtccggcc cgtgtggttt ccccgcctag
      481 gttgaccctg acgttcttcg aagcgctcct cgggtcttct atcttctcca tcctgaagag
      541 gagcttctgc ttgctcttgg cctggctggc cttgctcgac ttggcccggt tcttctctac
      601 aaagcgctcc agttgcgccc tgcgttcggc gttggccttg gctttgcggg cgagttgctc
      661 ttcgcgagcc cgtttctcgg ccacgtactt gctgtagttg ccagggtagc gggcgagacg
      721 gccaccttcg agttccagga tcgaaccggc aacggcgtcg aggaagtagc ggtcgtggga
      781 gacgacgagg acggcgctcc tcgccccctt gacgaagttc tccagccact ctatggcgcg
      841 caggtcgagg tggttggtcg gctcgtcgag gaggatgaga tccggctcct ccatcagcag
      901 gcgggccagg gctatgcggc tctgctcgcc gcccgagaac gagccgacgg gccgcttcca
      961 atcctcggga gcgaagccca aagaagtgat ggtcgaggct gcccgcgcgc ggtactcgta
     1021 gccgccgtac cgctcgaact cgctctgaag cctgccataa cgctccatta gcgcgttcga
     1081 cgggtcctcg gagaggcgcg tgccaagctc ctcgagctcc ttctcgcggg ccatcaaggg
     1141 ctcgaaggcg gagatgatct cctcctccag ggagtgcgcg cccctctcgc ccagataaag
     1201 cttctgggtc gtcatcccga ccttcgcgcc gcctggatgc tcgaccgagc cagagtcggg
     1261 ttcggagtgt cctgccagaa gatcgagcag ggtcgtcttc ccggcgccgt tgcgcccgac
     1321 cagcccgatc ttctccccgg cctccaccga gagcgtcgcg ccagagagta ccttcaggtc
     1381 tccaccgtgg tactttacaa gatctcttgc gacaatcacc ttcatggcac aagaattata
     1441 cagaggggac cccctgacgc cgggagagcg agaggagttc gaagacgacc tcatcgcctg
     1501 ctgcgaccgt gaactcgacc tcctcgggga cattacccgt ctgaacgttc tctacgcagg
     1561 aggatcctcg ctgctgtgga tcgaaggcct cagccagcgg ataggagacg gcggtagcgt
     1621 caccgccatc gacatggatg cggaacgcgt cgatgatacg caagcttctc ttggcgaggc
     1681 ggagcttgca gccccggtcc atcttctcgc gggcgacgtc ttagggatgc ccttcgagcc
     1741 ccacgccttc gacctcgtct actcctcggg cctgttccac gagctggacg taaaggagag
     1801 ggcggcggaa gatgcgctcg cggcgctcca cctcgtggca cagtccgggg gcagggtcgc
     1861 gacgagcgac ttcgtcgact ctgaaccggc ggtgcagctc gaagacgaga ggcttgaggc
     1921 gcagttgagg cgtgaggcgt acgcacagga gccattcggg atcgggccgc cacaacggct
     1981 cgtcgccctg cacgagaagc ttctcacgga cgtgcggtgg ctggtatcgc cgccacggag
     2041 aatccgtcac ctaggaaagg tggtcctggc cgagaacgag cccgcggaac tgtcctcgtt
     2101 gccgctggcg accgcgaaac ggttacgcga acgccgcgac gccatgcgca gtcggatcct
     2161 gggcgaaggc tacacgcgtc cggccaccct ttatgtcgaa ggtctggtac agaatgctta
     2221 gatctcgatc tcgaagttca tccaaatggg tgacgcgccg gttatgtatg ggtgccatca
     2281 ttcgtccggg gaggaagaca catctcgtca acgtcaggag gtccgtatga ccctcatcgg
     2341 actgagttca gtcgagaagt cttacggagg cagggccgtg ctacggggtc tgggggtgaa
     2401 ggtgaacgcg ggggcgagga tcgggctcgt cggcggcaac ggctctggca aatccacagt
     2461 gcttcgcatc ctcgcgggaa cggaggatgt cgacgcgggg gaggtgatcc ggcgccgggg
     2521 cttgagtgta gcctctttgc ctcagtacat ccagggcgac gaacgcacgc cgatggacgt
     2581 cgtacgcgcg tcccgcccag agatctcaga tcttcaggag gaactcgaag cgtgcgagga
     2641 gcagctcggc ttgcccaggg tggcggccga cctgcgcagg atgcagcggg tactggagcg
     2701 ccaggaccgg ctcctgcgcc gcttcacgga gctcggtggt cccggctttg tgggtgaagc
     2761 acgccgttac ctcaggtcgc tgggtcttgg tgatggcgac atctatcgtc cgatgagcga
     2821 actgagcggc gggcagcgca aactggcagt cctggcctcc tgcctcgccc gccgtcccga
     2881 cgtcttgctc ctcgacgagc cagaagctca cctcgatgcc gggcgcaggg agctgctcga
     2941 ggcgatcgtc cgcacgttcg atggcgccgt cgtcatcgtc tcccacgacc gctacctgct
     3001 tgacgagacg gttgtggaga tcgccgagct ggaagacggg aagatcacat cgtggcccgg
     3061 caactactcg gcgtacaccg tagcgcgtga gctcaagctc aaacggcagc agcaactcta
     3121 cgtcgctcag cagaaggaga tagcgcggct cgaagaggcg atccggcgct tcaagctctg
     3181 ggccagcatg gtggtaaacg aacgtcacat caagcaggcc cgcaacaagc agcgccagat
     3241 cgaccgcatg gacaaagtgg agcaccccgt gctggagcgg cgcaaaatag gactggagtt
     3301 ccgcggcagg gtaaggggcg gcaggaaggt gatcgaactc aaggacgtga gtatggcctt
     3361 cggcgaggac cctgtactta tcggggtaga ccttaccgtc tcacgcggcg agcgcctcgg
     3421 gatagtaggg cctaacggcg ctggcaagag cgtgctcgca aaagtcctct ccggcttgct
     3481 tcccccgacg gagggcgagc gatgggcagg cccatccata gatatcgggt tcctggcgca
     3541 gaatgacgaa ccgccaccag gcgcttcacc actcgggctc gtgcgagaca ccaagccgct
     3601 ctacgaggac gaggccgtga agctgctcgg acgtttcctg ttctgctacg agcaggtcag
     3661 ggaaccggtt acatccctga gtggtggaga gcggacccgg ttgcaactgc tcctgttgat
     3721 gctgagagaa ccgaatttcc tcgtcctcga cgagccgacc aaccacctcg acatagactc
     3781 gctcgagatc ctcgaagccg agctggaacg attcgcaggc acggtagtct tcgtctcgca
     3841 cgaccgctac ttcctcgacc gtatcgccga tggcatagtc gaggtcagcg aaggcgaagt
     3901 gcatagccac gaaggaggct acagcgactg gcgccgatca gcatttcagc atctcagcta
     3961 gtcagcacgc gccctgtcgg gcgctctcag cacttcagca tttcgacttg ttagcttgta
     4021 gcgtacgcga gtcctgactg aagtgctgat aggcggcagg gccgccatgc tgaccagcta
     4081 tctctccagg agtatcgtga cggggccgtc gttgacgaga gatacatcca tcatggcgcc
     4141 gaagcggcct gtctcgaccg ggtgaacgcc ggccgcgcgg agccgctcgc agaagtggtc
     4201 gaagaggcgt gtggcatcct cgggcggggc cgccccgacg aagctgggac gcttgccttt
     4261 gcgagtgtct gcaaggaggg tgaattgaga gactgcgagg atggcccctc cgacttctct
     4321 gacactgcgg ttcatcttcc cctcatcgtc ggacatgatc ctcagtcctg ctatcttctc
     4381 ggccagccag tcagcttcgg gttcgccgtc tccttgcgcg acgccgacca gcaagaggag
     4441 gcccgggcct atgccggaga tcctctctcc ttcgaccgta acggatgctt ctttgacgcg
     4501 ttggaggact acgcgcaaag tcttcagagt ggttggcggc ctgcgtagcc ggcatcgatg
     4561 tcgtgccagt actccaccct ttcttcgggt gggtgccagc agaggaagac gatgcgtccc
     4621 cttcgctcgt gggggaagtc gagcaggccc atatcaaggt ccttgaggac gacgccaagc
     4681 tccgtgatgc ggcctatcgc caggtagagg ttgtacgcct ctacgccgta ctctgaacca
     4741 gccttcccgc cgccgttcca gggcgcggcc cgcagaattg gctccatgtg cggggccttc
     4801 tcgcgcaggg cgtcgcggtg gagcgccgct tcgtccagga tctcacgcaa gctgggtagg
     4861 agggcgttcg cttcttcgac ggtgaagagc ttctggaagg tgggttccac gcctgggaag
     4921 ggctagcggc ccacggccag gcggtctgcg agcatgtcgg ggaggcctgc ggcccgtatc
     4981 ttggcctggg cctttcttat gtcgtactcg acaaagcggt atgtcacgcg ttcgccgtcg
     5041 gcgatcacgt acgaggcgaa ggtatcccca tcgcgcggct ggcctaccga gccggggtta
     5101 accaggtagg ggccgccaga gtggagatcc agggtgtgtc cccccaacat cgcgccgttc
     5161 ggcgaagggg ctacggcttt cacgtgcgtg tggccgaaat agcagatcgg gacatcaggg
     5221 tactcgctct tcaacatagc gaggttgtcg tacgcggaga tcgtgttgat gatgtactcg
     5281 tcgggatcac gtacggagcc gtggacgaag agcgcctctt cggactgcat catgcgcggc
     5341 ctcgtgcgca agaatttggc gttctcctcg ctcaagtgat tccgcgtcca cagcacggcc
     5401 gcggcggccg tcgggttgaa ccagttcagg tccgtctcca ggtcggttac ggccaggtcg
     5461 tggttgcccg agatcgtcgg catcctcaag gagcgcacca cgtcgcagca ctcgtccggg
     5521 ttcgccccgt agccgattac atcgccaagg cagtagatag tctccacttc ctcgggcacg
     5581 tcgccaagga gggcttctag tgcctcgagg ttgccgtgga tgtccgagat caccgcatac
     5641 atatagggtg cggcgtagtt tatacgctgg gtggacgatt tcaatcgccc taccgtctgt
     5701 tgaagcgccg caggcgttac ggtaacatcc ctgggcggtc cccaatcttg cacgggcctg
     5761 tagctcaggg gatagagcaa cggtttccgg ggccgtgtgt cgcaggttcg aatcctgcca
     5821 ggcccgctct tggggggatt gtacttcctc ctgacaaatt tccctgcaag cagttgatct
     5881 atcccctaat ctacaggccc gcgcaagagt ggacctgcag gggaccacca ccgcatctcc
     5941 gaagcctttg acagctctat tgacagcttg agaggagacg catctcgtga gcaagggaaa
     6001 acgaggtagg ggagagggct cgatctaccg ccgcaaggat ggccggtggg tcgggcaata
     6061 cgacgtcgat gggaagcgca ggtacatcta cggcaagacc cgcaaggagg tagccagcaa
     6121 gctaaccaag gccatggcag agagggacgc tggtatggtg ttcgatgcgc tctccctgag
     6181 tgtgggtaac tacctggaca gatggctcga ctccagaagg gatacgctaa ggaggcgtac
     6241 ctggactcgc tacgaggaga tagtaaggct gcacctcaag ccctccctgg ggaacaccaa
     6301 gcttgagaag gtaaatgccc tgcagatcca atccctctat cgtgccaagc tggaggaggg
     6361 tctctctccc aggacggtgc agatcatcca tgccaccctg tataaggccc tgaagcaggc
     6421 agtgaagtgg accctgctcc ccaggaacgt ggtcgactcc gtagaccctc ccagggtccc
     6481 caagggggag ataagacccc tgagcgagga gcaggtcaag aagctccttg agactgcaca
     6541 gggagataag ctcgaagccc tctacatact cgccgtccac acgggcatgc gctcgggtga
     6601 gattctgggg ttgaggtggc aagatgtaga tctacaggga gggacagttc aggtaaggcg
     6661 cagtgtcttt ggtggacgta cagaggctcc caagagtgca aatggcaaca ggagcatcag
     6721 gctgaccgag acgtctataa gggcactaca agggcacgag agggccagtg agtgggtctt
     6781 ctcaaccagc gtagggacac ccatcagtgt ccataacctg cacaaccgtt cctggaagcc
     6841 cctgctcgct agtgcaggtc ttccccacaa cacgaggttc cacgatctcc gtcacacttg
     6901 tgcaaccctg cttctaacca agggagttca tcccaagatc gtacaggaga tgctcgggca
     6961 ctcatccatc accataaccc tcgacaccta ctctcacgtc ctccccaaca tgcaggagaa
     7021 ggcggtagag gctatggagg atatcttcga ggatcacaag tagccaagat ctgattgccc
     7081 gggagggggc atactcctta ccactcaatc aatctattag aagataggga gagtggtatg
     7141 gagtattacc cccttccttc agtcactgaa agcaccttat tgagagaagg gagccctttg
     7201 gagcaaacgt ctgaatcttc ttacatcaga gaatagatcg aggtctatcc tgagtcagag
     7261 tatataaata gtctgaggat attgagtaga ttcgtccaga gaggaagcta ccgaagagga
     7321 ctagcgtgag taacagcgtc cgttctgttc taccggaaac tcaactggac agtctccatc
     7381 tggcataagc ggtactagtc cgttgatagg accaccagag gtaggcgcta cactgacgct
     7441 atagctgttc atggaag
//
LOCUS       DASTKM010000334        16165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_676767, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000334 DASTKM010000000
VERSION     DASTKM010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 16165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16165
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676767"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFQ10_13415"
     CDS             <1..505
                     /locus_tag="VFQ10_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9966387.1"
                     /translation="QSVMIVMITAKDAEEDKVRGFEAGADDYVTKPFGMKELVARINA
                     NLKRSETGGRGKIIEAGDLQLDTKNFTARAGGESLELRLKEFELLAALASTPGELRSR
                     EELAKEVWGHAGVGSSRTIDVHIRRIRAALEQKSSYEYIHTARGLGYRFEPKPRRPVE
                     SLSERQE"
     gene            509..1258
                     /locus_tag="VFQ10_13420"
     CDS             509..1258
                     /locus_tag="VFQ10_13420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9966388.1"
                     /translation="MTLERQNSARHVSPDELQRVNKIRRDFVANVSHELKTPATSLKL
                     LAESLEETLDEDPVQARLFASQLKKETERLAHLITDLLDLTRLESQESIDYPTLVDVR
                     SVLMTVLARMRRVARKKDITLQWKRFGKAAEYTVYGDETQLTSMFTNLVDNAVKYTPP
                     GGKVEVVGGSEESEITIRISDTGIGIPEGKIPRIFERFYRVDKARSKATGGTGLGLSI
                     VRHVAENHGGRVTVHSTPGEGSTFTVYLPRA"
     gene            complement(1379..2311)
                     /gene="thiL"
                     /locus_tag="VFQ10_13425"
     CDS             complement(1379..2311)
                     /gene="thiL"
                     /locus_tag="VFQ10_13425"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET9966389.1"
                     /translation="MNEFEVIRRISALLPPAPPEVLVPLGDDCAVVKIRDSTWVAASD
                     MLVSGHHFKDWATPEDVGYKAVAVNASDVAAMGGTPRFVLASGGAPEPETALRCFEGV
                     LEACERFGVYPLGGDTTRADALTVDVAILGELATQPVLRSGARPGDFLAVTGELGASA
                     AGILALEHSMTGPQRLIRRCLRPEPRLEAGRIAARLGATAMIDLSDGLASDVRHVCDR
                     SGVGCIVDLDLLPFKDDTRELAESLERDPDLLAATGGEEYELLICAPWSVLDALANSV
                     EIPLTVIGEITEGDVVFERGDERVEGLSGWNHFT"
     gene            complement(2308..2532)
                     /locus_tag="VFQ10_13430"
     CDS             complement(2308..2532)
                     /locus_tag="VFQ10_13430"
                     /inference="COORDINATES: protein motif:HMM:NF033684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HET9966390.1"
                     /translation="MGIVLLVAAVLFWVAAPAVLLTPLSTAQKAWTSSAFLVLGEVAF
                     WVAAVVLGREVFRRYRRFLDPRGWLGKKRS"
     gene            complement(2604..3476)
                     /locus_tag="VFQ10_13435"
     CDS             complement(2604..3476)
                     /locus_tag="VFQ10_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9966391.1"
                     /translation="MKADLVSGLLPIREDGRMLLLQRPTGTWEPPAGRLQPGESFGEG
                     AVRELYEETGVLVSPQRIIATWVGEAASGRRLASVTYAGRTDRVEVRLSEEHLDHRWV
                     TLGEWMSLPSWWSHENIKRIAGPVEAVRTVGAVREPPLPSLREDTGVVTANLGAGAVL
                     VDLGGVEPRALLLRRRKPPVGLWENPGGMLEDGEDFEACASRELYEETGVRARIVDAW
                     WARVEPWRNPEDPEFYAGVGFLARHQGGEIDPEAAAHDAARWVTETGWLSLRTWYTRR
                     ESDVLWKAIREMEP"
     gene            complement(3561..4328)
                     /locus_tag="VFQ10_13440"
     CDS             complement(3561..4328)
                     /locus_tag="VFQ10_13440"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HET9966392.1"
                     /translation="MHAAFRPETVLGRIEHRPYPVPSGPWVLGMSWRDLLFMHWPVGV
                     EDLRPLVSPALSIDTFEGSAWLGVVPFDMTGVRPRFLPAIPGLSHFPEMNLRTYVTAD
                     GRPGVWFFSLDAQSRVAVRLARATFHLPYFDAQMYCQASGDEVDFRSLRTHRGAPRAE
                     FAARYSPVGEPFESERGSIENFLTERYCLYSADGRGHVRRGEVHHHLWPLQPAEAEVS
                     TLAMTQQIGLKLPETDPILHFSKRLDVLAWLPRRIGS"
     gene            complement(4343..5023)
                     /locus_tag="VFQ10_13445"
     CDS             complement(4343..5023)
                     /locus_tag="VFQ10_13445"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET9966393.1"
                     /translation="MRAAIFLNGSPDSSYLLRSVAGRADLIVAADGGARFALEAGVVP
                     DLVVGDMDSLGEDLALEVERRGAFLERHPVRKDKMDGHLAVLAARGRGATAADLLCAV
                     GGRPGALFAVPHILLAAERIGLRSTVVADQGRMILVEAGSRMVEGEQQDSVSIFPLSG
                     PAAGVTLEGMEYPLENATLDPGDTLGFHNELIGTQARVSVREGALLIVHETEGPVRGH
                     TDSCGGTS"
     gene            complement(5025..5582)
                     /gene="thiT"
                     /locus_tag="VFQ10_13450"
     CDS             complement(5025..5582)
                     /gene="thiT"
                     /locus_tag="VFQ10_13450"
                     /inference="COORDINATES: protein motif:HMM:NF021062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HET9966394.1"
                     /translation="MNAFRDTRVLTEAALAIALAFVLGFVVLFKMPYGGSVSLEMIPL
                     ILLALRQGWKVGVVAGAAYGLLYLAIDFFVVHPVQLILDYPLAYGALGLAGLFKPTVR
                     GAVLGTTVAVLARFACHFLSGVVFFASVAPQGWNPYLYSAAYNAAYLAPSLGIALVVT
                     VVLLKALERAQPSPRQLYYRDSKTS"
     regulatory      complement(5620..5721)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5727..6245)
                     /locus_tag="VFQ10_13455"
     CDS             complement(5727..6245)
                     /locus_tag="VFQ10_13455"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9966395.1"
                     /translation="MYQSPWCAFRVDEVELPGGAQIEYGVLESGGFASMVPVTGGGDV
                     VLVRQWRQPLGAFTLELPSGVVDAGEKPERTARRELFEETGYRAERLEHLVSVHTSTG
                     RSTEVCHLFGCAAVRDERGPRPEPTEFIRVVELPFGEALQRVSEGGITDAATVLGLLW
                     SAGGAGAGGRLH"
     gene            complement(6443..6676)
                     /locus_tag="VFQ10_13460"
     CDS             complement(6443..6676)
                     /locus_tag="VFQ10_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET9966396.1"
                     /translation="MTYVITEPCIGTKDQSCVEVCPVDCIYDAGDHFMINPEECIDCG
                     ACEPECPVEAIYPEDEVPEDMESYTTKAADYFE"
     gene            complement(6827..7579)
                     /locus_tag="VFQ10_13465"
     CDS             complement(6827..7579)
                     /locus_tag="VFQ10_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9966397.1"
                     /translation="MYGENAPPSWARLPGARCRAVEHLTKLDGLKEFELFEGLEEGFF
                     QDLAIVAEVLELGADTALYREGEPSGAVYFVGEGRVKLYRSSGGPKARDQILGVVGDG
                     SVLGLGAALEGRTQTHGATALTECVLYAVFRDDLFEIMGRFPDGAVRLSRSLAARTRE
                     LEDLVGDLVFRSAPQRVARMLLNLATEEGRVTKRGVVFAPSLSRQEMAEATGISREAL
                     SRALSRLAKEGILDLGSKSITILMPAELRSRT"
     gene            7792..8592
                     /locus_tag="VFQ10_13470"
     CDS             7792..8592
                     /locus_tag="VFQ10_13470"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9966398.1"
                     /translation="MRFKDRVTIVTGGGSGLGRVLAHRFAAEGAAVVVADVARERAVA
                     VAEEIFDAGGDSLAQTTDVTDASEVKTMVEATREAFGPVDVLINNAAKATDADFLSLG
                     EGIWDEDVAITLKGPFLCAQAVLPDMVKHGSGVIINISSVNALGYYGNEAYSAAKAGI
                     LSLTRSLAVLYGPSGIRVNAIAPGTLRTPAWEQRREKNPYVFERLTRWYPLGRVGEPD
                     DVAGAALFLASDDATWITGAVLPVDGGLTAGNMQMMEEMIKGQGDQTS"
     gene            8654..9856
                     /locus_tag="VFQ10_13475"
     CDS             8654..9856
                     /locus_tag="VFQ10_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966399.1"
                     /translation="MLAFASDLHDEGVGTVLDNVQNRAGVNGLTMAVAYHDARDVFPH
                     NPVRKVRYLEGGAVFFPPDVARYGDLQPRVSELARSGDPLGELCEAAGERGMHVNAWA
                     VFLHSDRLGFSHPECATQNAFGDRYLTDLCPSNPGVRTYARTLASDIARYDVSTILSE
                     SVHFHGLRHGYHHERYFEEFGAVGLYLLGLCFCEHCLEAARRRGVDAGMVHRLVRDEL
                     ERRFSGGSSEDPEELRREDLEPFGGEEMLGYLDTRTETVTSLVGEVATAASGGGAGFA
                     VLDLCGAEKGFATGYPEGEAAPTLGWQMGIDQAELAGVCDTVAATGYAADPGRLNLDL
                     DAYQTLVPDASLLGLVLRPMPPDCRSADNLAAKVALARERGLGRLDFYHYGFCRLQAL
                     DWIQQALA"
     gene            complement(9905..10276)
                     /locus_tag="VFQ10_13480"
     CDS             complement(9905..10276)
                     /locus_tag="VFQ10_13480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HET9966400.1"
                     /translation="MRKTVMAEKGPAPAGPYSHAVVAGDFVFISGQGPVNPETGTMPD
                     AFTDQVRQTFENVKTILEASGSGLDNVVKVNAYVTDLTRFAEFNEVYKEYFDHDPPAR
                     TTIAAVLLGMLVEVDCIAVVE"
     gene            complement(10273..10410)
                     /locus_tag="VFQ10_13485"
     CDS             complement(10273..10410)
                     /locus_tag="VFQ10_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966401.1"
                     /translation="MWDPVLENGWLVDGISHVLGNGTKAVESGALMGTEKGKVIGKEH
                     R"
     gene            complement(10420..11532)
                     /locus_tag="VFQ10_13490"
     CDS             complement(10420..11532)
                     /locus_tag="VFQ10_13490"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9966402.1"
                     /translation="MVSRSVGEVSGGTPYVLIDGTKMERNVLKMAEVARVTGVALRPH
                     VKTHKIPGIAREQLEAGAAGITVAKLSEAEVMADGGIEDIFVAYPLVTDAKIRQAIRL
                     GERVRLVVGVDSLEGARRLSAVAEGHTLEVRLEVDTGLRRTGVLYDEAVGLAGEIEGM
                     DNLDLTGIYTYRGAVLGGSKTLELEKAGLEEGQLMVSLADMLRERGIRVDDVSVGSTP
                     TAEYVAKVEGVTEIRPGTYVFYDRMQARLGACSLDECAAVVVCTVVSRPARDLAIIDG
                     GSKTFATDVAPGAEPLNLEGYGHVVGYPDAVLERLTEEHGMLSVDEDCDLQVGDTVQV
                     IPNHICSTVNLHDAVYLVGEDGVVEETLVAARGKVR"
     gene            complement(12509..12847)
                     /locus_tag="VFQ10_13495"
     CDS             complement(12509..12847)
                     /locus_tag="VFQ10_13495"
                     /inference="COORDINATES: protein motif:HMM:NF022279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrdB family protein"
                     /protein_id="HET9966403.1"
                     /translation="MSILKGANLALRFLLELCALAALCYWGFNTGTGPISKVVLGIGA
                     PLAAAVLWGTFVAPAAPVSVPGALRLLLELAVFGGAAAALYSAGRPGLAWALGLAYVI
                     NRVLMFVWDQ"
     gene            complement(12857..13459)
                     /locus_tag="VFQ10_13500"
     CDS             complement(12857..13459)
                     /locus_tag="VFQ10_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966404.1"
                     /translation="MASWNLIRAGGGLASAAAGILLLVGHVLNLGGDPEYGTVPGASS
                     VLTAHVLLVFALVALYAAQAERSGVPGILGMVLGVAGTTLVSGVVLVEMAGASGANVE
                     AALGGGLAGTLALLGGLAFFLGLILFGAATMRAGVFPRWAGLLLIVGDVVFGAGDHFG
                     PAAPIVYVLGAAITGAGFVWLGLALLSGRGTSAQRPARVS"
     gene            14204..15247
                     /locus_tag="VFQ10_13505"
     CDS             14204..15247
                     /locus_tag="VFQ10_13505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9966405.1"
                     /translation="MNRNPALSVILAARDEERSVKESVVSMLDQDYSGLLEVIAVNDR
                     STDRTGEILEELATKHPDSLRVLNVESLPEGWLGKTHALYIGAAEATGEWLLFTDADV
                     IFSYDCSDKAVRYAIDNGLDHLTLPPEILCNGILLRSFVAAFTLVFEMTQRPWRVSDP
                     QAREHVGIGAFNLMKKVAYEKIGTHCAIRMRPDDDMKLAKLLKRHGFRQGVAYGTGLI
                     SVEWHQTLPGAVRGLSKSMFSGLDYRIGATVAGVLMLFLTNLLPAFGLFSRNTTGILC
                     RLNSFSTVLVYAYRAKHVGDKTPWWYAVLHPFGIGIFIYAMVRSASTTLVNGGIEWRE
                     TRYSLKDLKDNAI"
BASE COUNT         3117 a         5254 c         4892 g         2902 t
ORIGIN      
        1 ccagagcgtg atgatcgtga tgatcacggc caaggacgcc gaagaagaca aggtccgcgg
       61 gttcgaggcc ggcgccgacg actacgtgac gaaacctttc ggcatgaagg agctggtcgc
      121 ccgcataaac gcgaacctga aacgcagcga gacgggcggc cggggcaaga taatcgaggc
      181 cggggacctg caactggaca cgaagaactt tacggcccgc gccggtggag agtcgctgga
      241 gttgcgcctg aaagagttcg agctcctcgc cgccctcgcc tccaccccag gcgagctgag
      301 gagccgcgaa gagctcgcca aggaggtctg gggccacgcc ggcgtcggtt cctcgcgcac
      361 gatagatgtg cacatacgca ggatccgggc cgcgctggag cagaagtcgt cctacgagta
      421 catccacaca gcgcggggac tcggctaccg tttcgaaccc aagccaaggc gccccgtcga
      481 atctctttcg gaacgccagg agtagagggt gacgctggag agacaaaaca gcgcccgtca
      541 cgtcagcccc gatgagctcc agagggtcaa caagatccgg cgcgatttcg tcgccaacgt
      601 ctcccacgag ctcaagaccc cggccacgag cctgaagcta ctggccgaga gcctcgaaga
      661 gaccctcgac gaagacccag tccaggcgcg cctcttcgcc tcgcaactca agaaggagac
      721 cgagcgcctc gcccacctca ttacggacct cctcgacctg acacgcctcg agagccagga
      781 atccatcgat taccccaccc tcgtcgacgt gcgcagcgtc ctgatgaccg tgctcgcccg
      841 catgagacgc gtcgcccgca aaaaagatat caccctccag tggaaacgct tcggcaaggc
      901 agccgaatac actgtctacg gcgacgagac acaactcact tctatgttca ccaatcttgt
      961 agataatgca gtgaaatata cgccgcctgg gggcaaggtc gaagtggtcg ggggctccga
     1021 ggagtcggag ataacgatcc gcatctctga cacaggcatc ggcatccccg aggggaagat
     1081 cccacgcatc ttcgagcgct tctaccgggt ggacaaggcc cgctccaagg ctaccggcgg
     1141 aacggggctg ggcctctcca tagtgaggca tgtggccgag aaccacggcg gccgggtcac
     1201 cgtccacagc acccctggag agggatccac cttcaccgtg tatctgcctc gagcctagag
     1261 cgccttgctt cgcagagcac ggcatcagga gatctctcgc aaggctccga actactcgga
     1321 cgaatcaaga aggagttggg gtgcgtccag cgggcgctcg aggcagagct aacaggtttc
     1381 aggtgaagtg gttccagccg gatagtcctt cgacccgctc gtccccacgc tcgaagacga
     1441 catcgccctc ggtaatctcc ccgatcactg tgagggggat ttctacgcta tttgccaggg
     1501 cgtccaggac agaccagggg gcacagatca ggagctcgta ttcttcgcct cccgttgcgg
     1561 cgaggaggtc agggtcgcgt tcgagggatt ctgcgagctc tctagtatcg tctttgaagg
     1621 gcagtagatc caggtccact atgcatccca ccccgctccg gtcacacacg tggcgtacgt
     1681 ccgaagcgag gccatcggag aggtctatca tggcggtggc gcccagtctg gcggcgatgc
     1741 gcccggcctc gagcctcggt tcgggccgca ggcacctgcg gatgagacgc tgcgggccgg
     1801 tcatgctgtg ctcgagtgcg agaatcccgg ccgccgaggc gccgagctcg cctgtgaccg
     1861 ccaggaagtc gccggggcgc gcccccgagc gcagtaccgg ctgcgtggcc agctccccga
     1921 ggatggccac gtccactgtg agggcatccg ctcgggtcgt atcacccccg agcgggtaga
     1981 ccccgaacct ctcacaggcc tccaagacac cctcgaagca gcgcagcgcc gtctcaggtt
     2041 cgggcgctcc tccagaggcc agaacgaacc tcggggtccc ccccatcgcg gccacgtccg
     2101 acgcgttgac cgcgacggcc ttgtagccca cgtcctcggg cgtggcccag tctttgaaat
     2161 ggtggcccga gacgagcatg tcggaggcgg ccacccacgt actgtcccgg atcttcacaa
     2221 cggcacagtc gtccccgagg ggcacgagca cctcgggtgg cgcgggaggc aggagggctg
     2281 agatcctgcg tataacctcg aactcgttca agaacgcttc ttgcccaacc agccacgggg
     2341 atcgaggaat ctcctgtagc gccggaatac ctcccgtccg agcaccacgg cagcgaccca
     2401 gaacgctacc tctcccagga ccaggaaggc cgaactcgtc caggccttct gcgcggtgga
     2461 gagcggggtc agcagtacgg ccggggccgc cacccagaac agcacagcgg cgacgagcag
     2521 gactattccc acgagccgcg tcaggccagc ccttttcgaa acacgaggct cctggcccgc
     2581 tctttgcctg tacccttgct cgtctaaggt tccatctccc tgatggcttt ccagagcacg
     2641 tctgactctc gtctcgtata ccaggtacgc agcgatagcc acccggtttc cgtgacccac
     2701 cgagcggcgt cgtgggcagc ggcctcaggg tcaatctctc ctccctgatg ccttgccaga
     2761 aagcccacgc cggcgtagaa ctcgggatcc tctgggttgc gccagggctc gaccctggcc
     2821 caccaggcgt ctactatacg ggcccgcaca ccggtctctt cgtagagctc gcggctggcg
     2881 caggcttcga agtcctcgcc atcttcgagc atgccgccag ggttctccca gaggccaact
     2941 ggcggtttac ggcgcctgag cagtagcgcc cgcggctcga cacctccaag atcaacgagc
     3001 acggccccgg ccccgaggtt ggcagtgacc acgccggtat cctcgcgtaa ggatggtagg
     3061 gggggttcgc gaaccgcccc taccgtgcga accgcctcca cgggcccggc tatgcgcttg
     3121 atgttctcgt ggctccacca gctcggcagc gacatccact ctccgagggt tacccaccgg
     3181 tggtcgagat gctcctcgga gagacggacc tctactctat cagtccgtcc cgcgtaggtt
     3241 acggaggcca gacgtctacc gctcgccgcc tcgccgaccc aggtggcgat gatgcgctgg
     3301 ggagagacca ggacgccggt ctcttcatag agctcgcgga ctgccccctc cccgaagctc
     3361 tcgcccggct gaagacgtcc ggccggcggc tcccaggtac ccgtgggtcg ctggagcagg
     3421 agcatgcggc cgtcctccct gatgggcagc aggccggaga cgagatccgc cttcaaagcg
     3481 cgcccgtagt aattgcttcg ggtctactct ccactcggca agtatatggt ccacgggggc
     3541 ggttcgcgaa ccgctccggc ttacgagcct atccgtcgcg gtagccacgc gagaacgtcc
     3601 aggcgtttgg agaagtgaag gatcgggtct gtctcgggca gcttcaggcc tatttgctgc
     3661 gtcatcgcga gcgtgctgac ctcagcctcg gcgggctgga ggggccagag atggtggtgg
     3721 acttctccgc ggcggacgtg tcccctcccg tccgcgctgt agaggcagta gcgctcggtc
     3781 agaaagttct cgatggaccc cctctcgctc tcgaagggct cccccacagg gctgtagcgg
     3841 gccgcgaact ccgcccgcgg cgcgcccctg tgcgtcctca ggctcctgaa gtcaacctcg
     3901 tcgcccgaag cttggcagta catctgggcg tcgaagtaag gaaggtggaa tgtggcgcgg
     3961 gcgaggcgga cggctacgcg gctttgggcg tcaagggaga agaaccagac acccggcctg
     4021 ccatccgccg tcacgtaggt gcggaggttc atctcaggga aatgcgagag cccggggatc
     4081 gcgggcagga agcggggcct tacgccagtc atatcgaagg gtacgacgcc gagccaggcg
     4141 ctgccttcga aggtgtcgat gctcagggcc ggcgacacga ggggcctgag atcttccact
     4201 ccgacgggcc agtgcatgaa gagcaggtcc cgccagctca tcccgagcac ccacggaccg
     4261 gaagggacgg ggtagggacg gtgctctatc ctgccaagta ccgtctcggg tcgaaaagcg
     4321 gcgtgcatgg gcgcccgtaa tcttaggacg ttcccccaca ggagtccgta tgccctctca
     4381 cgggcccttc cgtctcatgt acgatcagaa gcgccccttc tctcacgctc accctggcct
     4441 gcgtgccgat caactcgttg tggaacccaa gggtgtctcc ggggtccagg gtggcgttct
     4501 ccaacggata ctccatgcct tcgagggtga cgccggcggc aggaccggac agggggaaga
     4561 tcgagacgct atcctgctgc tctccttcca ccatcctgga gccggcctcg accaggatca
     4621 tccgtccctg gtcggccacg accgtggaac gcagaccgat acgctcggcg gcgagcagga
     4681 tgtgtgggac cgcgaagagg gcccctggcc tcccgcccac ggcgcagagg aggtcagccg
     4741 cggtcgcccc ccgccccctg gcggccagta ccgcaagatg tccgtccatc ttgtctttcc
     4801 tcaccggatg gcgctccagg aaggccccgc gcctctcgac ttccagcgcg aggtcttctc
     4861 ccaacgagtc catatccccg acgacgagat caggcacaac cccggcctcc agcgcgaacc
     4921 tggctccacc gtcagcggcg actatgaggt ccgcacgtcc ggcgacgctc ctgagcagat
     4981 aggacgagtc aggagatccg ttcaggaaga tggcggcccg cacgctacga ggttttcgag
     5041 tcccggtagt atagttgcct cggcgaaggc tgcgcccgtt ccagagcctt cagcagcacc
     5101 acagtaacca ctagcgcgat gcccaggctc ggggccaggt acgcggcgtt gtacgccgcc
     5161 gagtagaggt aggggttcca accctggggc gcgacggagg cgaagaagac tacgcccgag
     5221 aggaagtggc atgcgaagcg cgccagaacg gccactgtgg tgccgaggac ggcaccacgc
     5281 accgttggct tgaagagtcc tgcgagtcca agggcgccgt aggccagagg gtagtccagg
     5341 atgagctgca caggatgtac cacgaaaaaa tcgatggcaa gatatagcag accgtaagcc
     5401 gctcccgcga cgacgcccac cttccagccc tgcctgaggg cgaggagtat caggggaatc
     5461 atctccaggg agacggagcc gccatacggc atcttgaaga gcacgacgaa tccgagaacg
     5521 aaggccagag ctatagcgag cgccgcctcg gtcagtaccc tggtgtctct gaaagcgttc
     5581 aactgcgcac cctccttccc gtcttcgggg atgcgccgat aagagactcc gagatctccc
     5641 tgcgccggca ttacccggat caggttcagc gggtcggcgc ctcggcgccc tctcagcctc
     5701 acggctcccc gggcttactg atgctctcag tgtaacctac cgccggcacc ggcaccaccc
     5761 gcactccaga gcaacccgag cacggtggcg gcgtcggtga tcccaccttc agagaccctc
     5821 tgcaaagcct ccccgaacgg caactccacc acccttatga actccgtagg ctcgggccgc
     5881 gggccacgct cgtccctcac cgccgcgcac ccgaaaaggt ggcagacctc cgtgctccgt
     5941 cccgtgctgg tatgtacgga gacaagatgc tccaatctct cggccctata gcccgtctcc
     6001 tcgaagagct ccctacgggc cgtccgctcg ggcttctcgc cggcatcgac cacgccgctc
     6061 ggcagctcca gcgtgaaggc gccaaggggc tgcctccact ggcgcacgag caccacgtct
     6121 cccccgcccg tgaccggtac catggaggcg aacccgccgc tctccagaac gccgtactcg
     6181 atctgcgccc cgcccggtag ctccacctcg tccacgcgga aggcgcacca tggactctgg
     6241 tacacgtact cccttccgag cgtccgccag ctctcttctc ccatgcggga aagtataaat
     6301 gcgatgtgca cagatattga cctgctgcga atcgcccatt cagctggagg cggaaacaag
     6361 aagatgacgt gctgaagtgc tgacaaacat gcaaaaagga ggggcctgag cccctcccca
     6421 aagaaaccct atgctctgtc ttctactcga agtagtcggc ggccttggtg gtgtagctct
     6481 ccatgtcttc ggggacttcg tcctccgggt agatggcctc gaccgggcac tcgggttcgc
     6541 aggcgccgca atcgatacac tcttcagggt tgatcatgaa gtggtcgccg gcgtcgtaga
     6601 tgcagtccac cgggcagact tcgacgcaac tctggtcctt ggtgccgata cacggctccg
     6661 tgatcacata ggtcaaaggg gcctcctctc tgggtgtctc gtaatggctt tataccagcc
     6721 cgccgccacc atcaacaccc gccttgatga gtgtcatggc agccgatgat catggtcata
     6781 cggtcgtgtg atcctggtca aaaaccgccc ttgctgcaaa ccgttctcag gtacgggagc
     6841 ggagttcggc gggcatgagt atggttatag acttgctgcc gaggtcgagg atgccctctt
     6901 tggcgaggcg agagagggcg cgtgagaggg cctcgcgcga gatgcccgta gcctcggcca
     6961 tctcctgccg gctcagagaa ggcgcgaaga cgacgcccct cttggtgacg cggccctcct
     7021 ctgtggcgag gttcaagagc atccgcgcca ccctctgtgg ggcgctgcgg aagacaaggt
     7081 cgccgacgag atcttccagc tcgcgggtcc tcgctgccag cgacctggag agcctgacgg
     7141 cgccgtcggg gaagcggccc atgatctcga acaggtcgtc gcggaagacg gcgtagagaa
     7201 cgcattcggt aagggccgtg gcaccatggg tctgggtccg gccttcgagg gccgccccga
     7261 ggccgagcac ggagccgtcg ccgaccacac cgaggatctg gtcccgcgcc ttgggaccgc
     7321 cggaagaacg gtagagcttc acgcgcccct cgccgacgaa gtacacggcc cccgatggct
     7381 cgccctccct gtacagggcg gtgtctgcgc ccagctcgag cacctcggcc actatcgcga
     7441 gatcctggaa aaatccctct tcgagccctt cgaagagttc gaactccttg agcccgtcca
     7501 gcttggtcag gtgctcgacc gccctgcaac gcgcgcctgg caaccgtgcc caggaaggtg
     7561 gcgcgttctc accgtacatc gttctgtgtt ccttccggcg catgtagtct cacgtaccct
     7621 ttatacgcta agtgaacaaa gtttgcactg agagcgcttg aggtatcagg attagcacgg
     7681 gatagtccta cttgctgagg agggctgctc tgtcggggag tgtactccac gggggtttag
     7741 ggacgatcgt ttcatctata ttgatgccgg cagaggggat cggaggtaga gatgcgcttc
     7801 aaggataggg ttaccatcgt gacggggggc ggttcgggac tgggtcgggt actggcgcac
     7861 cgattcgcag cggagggcgc tgccgtcgta gtcgctgacg tagcgaggga acgcgcggtc
     7921 gcggttgcgg aggaaatttt cgatgcgggt ggcgactccc tggcgcaaac gacggacgta
     7981 accgacgcgt ccgaggtcaa aaccatggtc gaagcgaccc gcgaagcgtt cgggccggtg
     8041 gatgtcctga tcaacaacgc ggcgaaggct accgacgccg actttctcag tctcggcgag
     8101 ggcatctggg acgaggacgt ggcaattacc ctgaagggtc cgtttctgtg tgcgcaggcg
     8161 gtgctccctg acatggtcaa gcacgggtct ggggtcatta tcaacatcag ctcggtgaac
     8221 gcccttggtt actacggtaa cgaagcctac agcgcggcca aggccggcat cctcagcctg
     8281 acgaggagcc tcgctgtcct ctacggacca tccggtattc gcgttaacgc catcgcaccc
     8341 ggcaccttga gaacccctgc ctgggaacag cgaagggaga agaaccctta tgtcttcgag
     8401 cgtctcacca ggtggtatcc gctgggccgc gtcggtgagc ccgatgacgt ggccggggcc
     8461 gccctcttcc tggcatcgga tgatgcgacc tggatcaccg gcgccgtact ccccgtcgac
     8521 ggaggactca cagccggcaa catgcagatg atggaagaga tgatcaaagg ccagggcgac
     8581 cagacgtcgt aacgcggagc gccttgatcg caagcccccg gatagatagt ctgttgccct
     8641 gatggagacg tcgatgctcg ccttcgcttc cgaccttcac gacgaaggcg tcggtacggt
     8701 cctcgataac gtccaaaaca gggcaggggt aaacgggctg acgatggccg tggcctacca
     8761 cgacgccagg gacgtctttc ctcacaaccc cgtccgtaaa gtgcgctatc tggagggtgg
     8821 cgccgtcttc ttcccccctg acgttgctcg ctacggggat ctacagcccc gcgtcagcga
     8881 gctcgcccgc tcgggggacc ctctcggcga gctgtgcgag gccgccggcg agcggggaat
     8941 gcatgtaaac gcctgggccg tattcctgca cagcgaccga ttgggcttct cgcacccaga
     9001 gtgcgccacg cagaacgctt tcggagatcg ctacctaacc gacctgtgcc catctaaccc
     9061 cggcgtgcgg acgtacgcgc gcacgctcgc ctcggatatc gcccgctacg atgtctcgac
     9121 aatcctctcg gagtcggtcc acttccacgg actcaggcac ggctatcatc acgaacgcta
     9181 tttcgaggaa ttcggggctg tcggcctcta tctgctcggg ctgtgcttct gcgaacattg
     9241 cctcgaggcg gcccgccgta gaggagtcga tgccgggatg gttcacaggt tggtacgcga
     9301 cgaactggag cgtcgctttt caggcgggag cagtgaggac cccgaggagc tgaggcgcga
     9361 ggatctcgaa cccttcggcg gcgaggagat gcttgggtat ctcgacacga ggacggagac
     9421 tgtgacgtcg ctcgtaggcg aggtggcgac ggcagcctcc ggcgggggcg ccggtttcgc
     9481 ggtcctggat ctctgcggcg cggagaaggg tttcgcgacc gggtatcccg aaggcgaggc
     9541 cgcacccacc ctcgggtggc agatgggcat cgaccaagcc gagctggctg gtgtgtgcga
     9601 caccgtcgcg gcgaccggat acgcggccga ccccggcagg ctgaacttgg atctcgacgc
     9661 ttaccagacc ctggtccccg acgcctcgct gctcggtctt gttctccgtc cgatgccgcc
     9721 ggactgccgg tcggccgaca acctggccgc gaaggtcgcc ctcgcgcgcg aacgtgggct
     9781 aggacgtctc gacttctacc actacggttt ctgccggttg caggctctgg attggatcca
     9841 gcaggctctt gcctgatggc aggtatcagg cttcaggtct tgattgtggt gctagcggtc
     9901 cggactattc gaccactgct atgcagtcca cctctacgag catgccgagc aggacagcgg
     9961 cgatggtcgt ccgtgccggg gggtcgtggt cgaagtactc tttgtacacc tcgttgaact
    10021 cggcgaaccg tgtgaggtcg gtcacgtaag cgttgacctt cacgacgttg tcgaggcccg
    10081 aaccggaggc ctcgaggatc gtctttacgt tctcgaaagt ctggcgtacc tgatccgtga
    10141 aggcatctgg catggtgccc gtctccgggt ttacaggacc ttgccccgag atgaacacga
    10201 agtcgcccgc gaccacggcg tgggagtacg gtccggcggg cgccgggccc ttctctgcca
    10261 taacagtctt tctcatctat gctccttccc tatcactttg cctttttcag tccccataag
    10321 tgcgccggat tctacagctt tcgtcccgtt gccgaggacg tgagagatcc cgtccacaag
    10381 ccacccattc tcgagcacgg ggtcccacat cgcagcctcc taccgaacct tcccgcgggc
    10441 cgccaccagc gtctcctcca ccacgccatc ctctccaacc aggtacaccg catcgtgcag
    10501 attcaccgtg ctgcagatgt ggttggggat tacctgcacc gtgtcgccca cctggaggtc
    10561 acagtcctca tccacagaga gcatcccgtg ctcctccgtg agcctctcca gcactgcgtc
    10621 ggggtatccg acaacgtggc cgtacccctc caggttcagg ggctccgcgc cgggggctac
    10681 atcggtggcg aaggtcttgc tcccgccgtc gatgatggcc aggtcccgag caggcctgct
    10741 taccaccgtg cacaccacga cggcggcgca ttcgtcgagg gaacaggcgc cgagtcgggc
    10801 ctgcatccta tcgtagaaca cgtaggttcc tggccggatc tccgtcactc cctccacctt
    10861 cgccacatac tccgccgtcg gggtagagcc aacgctgacg tcgtccaccc ttataccccg
    10921 ttccctcagc atatcggcca gcgagaccat aagttggccc tcttcgagac ctgccttctc
    10981 gagctccagc gtctttgagc cgccaagaac ggcaccgcgg taagtgtaga tacccgtgag
    11041 atccaggttg tccatgccct cgatctcacc cgccagcccc acggcttcgt cgtaaaggac
    11101 cccggtgcgc ctcaggcctg tatccacctc gagcctgacc tcgagcgtat gaccctccgc
    11161 cacagccgag agcctcctgg caccttcgag gctgtctacc ccgacgacga gcctcaccct
    11221 ctcgcccagc cggatcgcct gacgaatctt cgcatccgtc accagcggat aggcgacaaa
    11281 aatgtcctcg ataccaccgt ccgccatcac ctcagcctcg gagagcttgg ccaccgtaat
    11341 gcccgccgcc ccggcctcca actgttctct ggctatgccc gggatcttat gcgtcttcac
    11401 gtggggtctc agcgcaaccc cggtcaccct ggcaacctcg gccatcttca gtacgtttct
    11461 ctccatcttc gtgccgtcga tcaaaacgta aggcgtccca ccagagacct ctccgacact
    11521 cctgctcacc atccgaatcc cctaccagaa cgaagagaac cagtcaagac gccccgctga
    11581 acgacgaata ccgcaccggc ccgaaaattc cgaagccagt cggtcgtcat tgatcccatc
    11641 gacccacttc acgacgctcc ccgtttcgcc agccatcggc atcccctgtc ccctcatccg
    11701 tgtacccttc tcccgcacgg acgagtacca atgctagcat gtttcgtcta tttccttttg
    11761 gcgtcatcca gggcaataca ggggtcttca gcgatccact ggttggcacc cgcttaatcg
    11821 ggttagatca cgcacggtac gtaatggacg gcgagttttg ttctcaatct gccgaaggaa
    11881 tctgctagct tcgtcggttc acagaaggtt gagacacccc tagaggtctg tgtacttcct
    11941 gttcgatcca tacgacttct ccagtggata cttccgttga gcttgaacat gaatggggac
    12001 cctggttcta gcccgacaaa gggagctcta ctgctaggct accagaagtt cacctcgtcc
    12061 accccagcac ggctcagaaa atcgaaccaa tcattatccg tcactcctac ccacagcttc
    12121 atcgcagtac ttgagaggaa aacatcaaca cacggaggcg cgtgcaggac gccgccgggg
    12181 gaccggccgc aaccgtgtct cgctgcctcc tagtcaggat agtagctggg ctctctcgcg
    12241 ttcgctcagg gtgcacagct cctcgtagat caaagttgtc cgccctgttc acgcccgtaa
    12301 ttcgattgcc ttcttcgagc aactcgtgat cgaggttgat ggtggttcgc atggtgctcc
    12361 tctccctcgg cacacaagct tgagcttcgt atcatgggac attctgggtt cgagcaccac
    12421 acctagttct caattcttga agggattttc gatcgtcagt ccttcgatcc gctgcccgtc
    12481 cggggtgctt gcgctccagg ctcgggcatc actgatccca cacgaacatc aggacacggt
    12541 tgatcacgta agccaacccc aacgcccagg caagcccagg acgcccggca gagtacagcg
    12601 cggccgccgc gcctccgaac accgccagct ccaggagcaa acgcagcgct ccgggcaccg
    12661 ataccggagc ggccggagcc acgaaggtgc cccagaggac cgcggctgcg agcggggcgc
    12721 cgattcccag cacgaccttc gagatcggtc cggttccggt gttgaagccc cagtaacaga
    12781 gggcggccag ggcacacagc tccaggagaa agcgcagggc cagattggca cctttcagta
    12841 tcgacacgcc ggcctcctaa ctcacacggg cgggtcgttg tgccgacgta cctctccccg
    12901 agagcagcgc caatcccaac cacacgaatc ctgcgccggt gatcgccgcg cccaggacgt
    12961 agacgatcgg cgcggccgga ccgaagtgat cccccgctcc gaacacgacg tcaccgacga
    13021 tcaggaggag tccggcccag cgtgggaaca cgcccgcccg catagtagcc gcgccgaaca
    13081 ggatgagccc gaggaagaac gcaaggccac ccagaagggc aagcgtgccc gcgaggcccc
    13141 ctccgagcgc ggcttccacg ttcgcgccgg aagccccggc catctcgacc aaaacgacgc
    13201 ccgagacgag cgtcgtgccc gccacgccga gcaccatccc caggatccca gggacgccgc
    13261 tccgctcggc ctgggctgcg tagagcgcga cgagggcgaa aaccaggagc acgtgggcgg
    13321 tgagcaccga gctcgctcct ggcaccgtgc cgtattcagg atcgccgccg aggttcagca
    13381 cgtggccgac caacagcagg atgccggcgg cggcgctcgc cagcccgcct cccgctcgta
    13441 taaggttcca tgaagccaag gtgatctccc tacttgcccg gcggtgaggc ctatggggca
    13501 agaccccgtc gaaccgccag cccgtctctc gagcagccta ccaccagacg gaagaggtta
    13561 tctagcgcac gtcactcggc tcgggtccgg catcgaggtc ggcgctgttg ctcactgcct
    13621 gctttatctg cgtgttatgg aggcgaaagg aaggggattt ctgtcacccg ggggtatacg
    13681 tgcgaggatg tgggtatcat gctcctgtcc acccgcccat agccaagcgc aagctcggcg
    13741 cgagacggag agcaacgcca cgccggcggc acagagctgg aggcgagagc caagccgaac
    13801 ccattcagga taccggcccg gtctcttcca gagtggagaa gccacggcta ggaaggtgca
    13861 acatgaccac gcagcaattc gaccaggcaa aggctgaagc cttcgcccag cgaccgatga
    13921 ggagcgttga atgctgtttg gtaaagctcc cactacctga ggcctcgggt ggaaggaaag
    13981 tcgtgcttga cccacccagc aggcactcta gctctggtag tctcgcagca ggtatgcaag
    14041 agcgtgcctc gtgagcgcct ttggagatca caaacacaga atttcgagaa ctctcagttt
    14101 cctcgcagcc ttcggctcac tggtatggtt ggttttgggc ctccagtggt tccgtggcac
    14161 gaggaacgta cccttgctca aagactttcg caagccgggt cacatgaacc gtaatccggc
    14221 actttcggtg atcctggccg cccgcgacga ggagcggtcg gtgaaagaga gcgttgtatc
    14281 catgttggat caagattatt cgggcttgtt ggaagtcata gccgtcaacg accgctctac
    14341 cgatcgtacc ggcgagatcc tggaagagct ggccacgaaa caccccgaca gccttcgggt
    14401 actgaacgtt gagtcattgc cggagggatg gcttgggaag acccacgccc tgtatatcgg
    14461 tgccgcggag gccacaggag aatggctcct gttcaccgac gccgatgtga tcttctctta
    14521 cgactgctca gataaagcgg tacgctacgc gatcgacaac ggattggatc acttgaccct
    14581 gcctcccgag atcttgtgca atggcattct gctccgaagc ttcgtcgcgg ccttcacact
    14641 agtcttcgag atgacccaga ggccctggcg ggtgagcgat ccccaagcca gggagcacgt
    14701 gggtatcggg gccttcaatc tcatgaagaa agtcgcctac gagaagatcg gaacccactg
    14761 cgccatccgc atgcgccccg acgacgacat gaagctggcc aagcttctca agaggcacgg
    14821 cttcaggcag ggagtggctt acgggacggg actcatcagc gtcgagtggc accagaccct
    14881 gccgggtgcc gtgcggggac tgagcaagag catgttctcc ggccttgact acaggatcgg
    14941 tgcgacggta gccggcgtgc tgatgctctt tctgaccaat ttgctcccag ccttcggtct
    15001 cttctcgcgc aatacgacgg gaattctctg caggttgaac agcttctcga ccgtcctcgt
    15061 atacgcttac cgagccaagc acgttgggga caaaacgccg tggtggtacg ccgtgcttca
    15121 cccctttggt atcggtatct tcatttacgc tatggtccgg tccgcatcaa ccactctcgt
    15181 caacggggga atcgagtggc gggagacaag atattcactg aaggacttga aagacaacgc
    15241 gatctgatca gagagtgtac aggaccgcct cagcccccaa caatccccaa ctcgatgaga
    15301 gggtctgcga ccacgcacac cgccgacgta attcccgccc acactccgat ggacctgacc
    15361 tcgtttgaac acaatcgagt aagtgtccaa aagggttcgg ggtgttctgg ccaaaggatg
    15421 ttgtcttggg ttctgttctt gttcagtatc tcggtttagg gcgctcacta gatctacgtc
    15481 gaactgcatg ttcagctctt cgccttcgaa gtcatgcctt aggctcgcga ttggggtatt
    15541 gggtttgatg tcgtagaaat cgaccttagc catgaatgtc cattctacag cgggttgcgg
    15601 tgacattgaa cgatgcacgc cgtgcatatg agagcggttg agcacaccat ccgacgaggg
    15661 ttatgcacgc atcaagctgc gtgggtcaac cacactgaaa accgctgcag gtatgaagaa
    15721 tcgttaagat cgtcgcgtgg tgcccgccat ggtgaatgtt cgggggctcc catacgtaat
    15781 acgggatagc atgccaccaa ctggcgaaga gaatgatgag cagacccgcg cttaccggaa
    15841 gaaagatgaa cgctgaggaa gcggatccgg cccgtggagc cgccggccga ccgcgacagg
    15901 acgtcggagc gagcgccaac ttccgtgtcc gggtcagcgg cggtaacttc tgctttgaga
    15961 gcggcggctt tgactaccgc ggtggctgtg gtggcggtag ccagggcggt ggtagacggt
    16021 aacgcggcgt aaaatgaagc ggctcttagc agggggggcg taccgaccaa ctgggcaagt
    16081 caggaggcat aagaaggcgg acggacgcac cttcgctcga cagcgaagcg ggactgcgtg
    16141 ccgcctacga cgcgctcgga agcga
//
LOCUS       DASTKM010000335         3849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1030567, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000335 DASTKM010000000
VERSION     DASTKM010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030567"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1251)
                     /locus_tag="VFQ10_13510"
     CDS             complement(136..1251)
                     /locus_tag="VFQ10_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9966406.1"
                     /translation="MPIFEYRRAFEIARHIKAFLEVGLTRNGSRDSRKPESRDAGSGG
                     SQRPVAGEELNGLRAGSTPDEDIPLFFLIGRSKSGTSWLMRLFNSHPEILCRGEGKFF
                     GKGKSNALHGALARSKEFNRWLGHNPWTLRDQDPNLDDIVANTINYLMQEKLRKTNKK
                     IVGDKSPFTTPGVVEEISSICPGAKVVHIVRDGRDVAVSSVYHQWNNATDQGGRRKLS
                     REKVEKREAYRKDPETFGASGESIFAGGHVAEIARSWSKSVSTAMKDAALLGDNYCQV
                     RYEDLLAEPVGEVMRLLEFLGADSDEEVARRCVEAASFEQLSGGRTQGEEASSSFYRK
                     GIAGDWKNHFTEEDRRVFKEEAGELLIRLGYEDDLDW"
     gene            1429..2595
                     /locus_tag="VFQ10_13515"
     CDS             1429..2595
                     /locus_tag="VFQ10_13515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9966407.1"
                     /translation="MKLSLCIATKNSAEYIEKLLRLGRSFADEVVVAVDASSNDLTQR
                     ICYENADRVFSVESPGYVERILPWLNEQCKGDWILRLDDDELPSVGLIEILPALMRDR
                     EMTHYHLRRRTMIGEDRTQWIAQRPWWPDWKLRLFRNIPSIVRIPGRLHTGYEVKGAS
                     RFLYRGSIYHYDFVYHGEQHRKEKVRQYEIISPEKSGSHHYSLPDEDALVTRPIPADD
                     EPADDPVESRPLAARAAIRHVDLAEIDLAAVQDCEYGPTLFRARLECTDCPRLMEAGR
                     WYKLDVDLRNDSPVAWPLPETGSPDVRITYHWLRADGDLYDYKGRRTGLPHMLRPGET
                     TRLPAPVRSPKEPGDYILQWDMVIPQVAWFSTQGWRGPEIPVSVEGASRPFQPG"
     gene            complement(2583..3656)
                     /locus_tag="VFQ10_13520"
     CDS             complement(2583..3656)
                     /locus_tag="VFQ10_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9966408.1"
                     /translation="MPVFDSRTVIELARGIRAFLKLEAGLTRGEIPARDQDVSEQPDS
                     HAGELARVRMRLEEKEREIAGLKQRLAAPAPMADGVSPENVVWIFCSNRSGSTWLSSM
                     MGEVEGHEVWNEPLVGKLFGDLYYVGAAGHEKVKHYILGDYHKGSWLDSIQSFVLSEA
                     TARFPEVAGRGYLIIKEPNGSIGAPLLSEAMPASRVIFLVRDPRDVAASGLDAARKGS
                     WQYENAADRGWKREALADNQPDVWVRRRAQNYVRHAGKARDAYEAHKGPKVLIRYEEL
                     REDALGTMERLYSTLGIQVREEELARAVEKHAWENIPEKEKGQGKFYRRAKPGGWRED
                     LTPAQVEIVEQVSAPLLKEFYPG"
BASE COUNT          691 a         1234 c         1113 g          811 t
ORIGIN      
        1 gccggcgaac acccggcgcc acgctctgcg taccctggcg taagcacgcc ccgacgctcc
       61 ctgctggggc acgagttccc tagtctggtg tgcgctcacg tgtttcccca aacctgcacg
      121 cgtccggtga gagtgctacc agtcgaggtc atcctcgtaa ccgagcctga tcaagagctc
      181 cccggcctcc tctttgaaga cgcgcctgtc ttcctcggtg aagtgattct tccagtcacc
      241 cgctatcccc ttgcggtaga aagaagacga ggcttcctca ccctgggtcc tgccgccaga
      301 gagctgctcg aagctcgcgg cctccacgca ccggcgggcg acctcctcat ccgagtccgc
      361 ccccaggaac tccagcagcc gcatgacctc accgaccggc tctgcaagga ggtcctcgta
      421 gcgtacctgg cagtagttgt cgccaaggag agcagcatct ttcatggccg tactcacgga
      481 cttactccag ctcctggcta tctccgccac gtgtccgccg gcgaagatgc tctcccctga
      541 ggctccgaag gtctcagggt ccttacggta ggcttcacgc ttttcaacct tctcgcgaga
      601 gagcttgcgg cgaccgccct ggtcggtcgc attattccac tggtggtaga ccgaggacac
      661 agccacgtcc ctgccgtccc tgactatgtg cacgaccttc gcaccagggc agatagagga
      721 gatctcctcg accacgccag gggtggtgaa cgggctcttg tccccgacga tcttcttgtt
      781 cgtcttcctg agcttctctt gcatgaggta gtttatggta ttggccacga tgtcgtcgag
      841 gttggggtcc tgatcccgga gggtccaggg gttgtggccc agccacctat tgaactcctt
      901 cgaacgggcg agggcgccgt gcagggcgtt ggacttgccc ttcccgaaga acttgccctc
      961 ccccctgcag agtatctctg gatgggagtt gaagagcctc atcaaccagg aggtccctga
     1021 tttcgaacgt cctataagga agaacagggg tatgtcttcg tccggagtag accccgcccg
     1081 cagcccgttg agttcttcgc cagctacagg ccgctgagaa ccccctgatc ctgcatcgcg
     1141 gctctcaggc ttccgagagt cccgactccc gttgcgggtg aggcctacct cgaggaacgc
     1201 ctttatgtgg cgcgctatct cgaaggccct gcggtactcg aagatcggca attgatattt
     1261 ctcctatcga gctacgttgc tactacaaag tacgatgctc tcacaaaaac ccgattccta
     1321 ctagtggcgt cggagcctcc tcccacagga cggtatctcc ggcacatctt gatagactgt
     1381 cacgaatcgt cgttgttcgc cggggtgaga tggcgttggg aggtgtgggt gaagctctcg
     1441 ttgtgtatag cgacgaagaa ttcggccgag tatatcgaga agctcttgcg actcgggaga
     1501 agcttcgccg acgaagtggt cgtggccgtc gacgcctcga gcaacgacct cacgcagaga
     1561 atctgctacg agaacgccga cagggtcttt agcgtagagt cgcccggcta cgtggagcgg
     1621 atcctcccct ggctcaacga gcagtgcaag ggggattgga tcctccgcct cgacgacgat
     1681 gagctcccca gcgtgggttt gatcgagatc ctgcctgccc ttatgaggga tcgtgagatg
     1741 acccattacc acctgagacg gcgcacgatg atcggagaag atagaacgca atggatagcc
     1801 cagcgcccgt ggtggcccga ttggaagcta aggttgttcc gcaacatccc ttcgatcgta
     1861 cggatccccg gtcgcctgca cacaggatac gaggtcaagg gggcttcgcg ctttctttac
     1921 aggggttcga tctaccacta cgatttcgtg tatcacggtg agcagcatcg caaggagaag
     1981 gtacggcagt acgagatcat atcgcccgag aagagcggca gtcatcacta ttcccttccc
     2041 gatgaggatg cgctggtcac gcgcccgatc ccggccgatg atgagcccgc ggacgatcct
     2101 gtcgagagca ggccgctggc cgcgcgggcc gcgatcaggc acgtagacct ggccgagatc
     2161 gacctggcgg ccgttcaaga ttgcgagtac ggccccaccc ttttcagggc gcgtcttgag
     2221 tgcacagatt gtccacggct catggaagcg ggacggtggt acaagctaga cgtcgatctg
     2281 cgcaacgata gccccgtggc gtggcctttg cccgagacgg gctcccctga cgtgcgcata
     2341 acctaccact ggctcagggc cgatggagac ctctacgact acaagggccg ccggacgggg
     2401 ctaccgcaca tgctgaggcc gggtgagacg accaggctgc ccgcgcctgt gcgctccccc
     2461 aaggagccgg gagattacat cctccagtgg gatatggtca tcccgcaggt cgcctggttc
     2521 tccacgcaag gatggcgagg acccgagatc cctgtgagcg tcgaaggtgc atcacgacct
     2581 tttcagcccg ggtagaactc ctttagcagg ggggccgata cctgctccac tatctctacc
     2641 tgagcgggtg tgagatcttc cctccagccg cccggcttcg ccctgcggta gaatttcccc
     2701 tggcccttct ctttctccgg tatgttctcc caggcgtgct tttcgaccgc ccgcgcgagt
     2761 tcctcttctc tgacctggat ccctagcgtc gagtacaggc gctccatggt gccgagtgca
     2821 tcttcgcgca gttcctcgta gcggatcagg accttggggc ccttgtgggc ctcgtacgcg
     2881 tctctggcct tcccagcgtg gcgcacataa ttctgggccc gacgcctcac ccagacatca
     2941 ggctggttat ctgccagcgc ctccctcttc cagccgcgat ccgccgcgtt ctcgtactgc
     3001 cagctaccct tcctggcggc gtccaggcca gacgcggcga cgtccctggg gtcgcgtacg
     3061 aggaagatca cacggctcgc cggcatcgct tcggagagca gcggcgcgcc tatggagccg
     3121 tttggctctt ttatgatcaa gtagcccctc ccggccacct cgggaaagcg cgcagtcgct
     3181 tcgctgagaa cgaaggattg gatggagtcg agccagctcc ccttgtgata gtccccgaga
     3241 atgtagtgct tgaccttctc gtggcccgcg gctccgacat agtacaggtc gccgaagagc
     3301 ttcccgacca gcggttcgtt ccacacctcg tggccctcga cctcgcccat catcgagctg
     3361 agccaggtgc ttccgctacg gttggagcag aagatccaga cgacgttctc ggggctgaca
     3421 ccgtcggcca taggagcggg cgctgccagc ctctgtttta gcccggctat ctctcgctct
     3481 ttctcttcga gtcgcatgcg gacccgcgcg agctccccgg catgggaatc gggctgctct
     3541 gatacatcct gatctcttgc cggtatctcg ccgcgggtaa ggccagcctc caacttcaag
     3601 aacgctctta tgccccgcgc taactcgatg accgtgcggc tgtcgaaaac cggcatcagg
     3661 ggtccttcct gttacggagt gtcgccgttt cagtgagcat tatgctagat cttgcctacc
     3721 aatccaggtc tctctcgtaa ccgagccgga tcaagagttc ccccgcttcc tctttgtaga
     3781 tctccttgtc gcgctcggtg aagtagttgt tccaatcacc cgcaacgcct ttacgataga
     3841 aggaggacg
//
LOCUS       DASTKM010000336         5614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1558251, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000336 DASTKM010000000
VERSION     DASTKM010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558251"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFQ10_13525"
     CDS             complement(<1..270)
                     /locus_tag="VFQ10_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966409.1"
                     /translation="MATTQKTNRAVERLAESTRDSYQTVVDHAVGLQERNVRFAQGIV
                     DGTIKELRHQAESNRSVVGELFERAEDQRDAYQTLVEQSIDAYMDL"
     gene            complement(301..711)
                     /locus_tag="VFQ10_13530"
     CDS             complement(301..711)
                     /locus_tag="VFQ10_13530"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9966410.1"
                     /translation="MNNEEQERETRGREREFRGVEARPRNWLVPVILLSLREWNSYGY
                     ELMERASAFGFEAMNPGTLYRTLRQMEKEGIVESSWETSRGGPARRMYTITDAGRSYL
                     DFWAKSLEQYQHTMDNFFRLYTGRPLKDKEESGE"
     gene            1208..1957
                     /locus_tag="VFQ10_13535"
     CDS             1208..1957
                     /locus_tag="VFQ10_13535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET9966411.1"
                     /translation="MGTLKEAVVVVTGASRGLGKAIAEELSGGGAKVVVNYSRSKEPA
                     EELVNQISESGGEAIAVQADVSDAEQAQKLIDQALERFNRVDVLINNAGINIDRTLRK
                     LSVEDWDKVIQVDLNSAFYTVHAVLPHMTDQGGGKIINMSSFVGEAGNIGQANYSAAK
                     AGLLGFTKTAAQELARFGITVNAIAPGFIETDMVANIPEEAQQKLRKQVPLGRFGKPE
                     EVARAVRYLIEDGDYITGQSIDINGGIYIRT"
     gene            2203..2970
                     /locus_tag="VFQ10_13540"
     CDS             2203..2970
                     /locus_tag="VFQ10_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HET9966412.1"
                     /translation="MRLDGRTAVVTGAARGIGAAEAIRLAQEGANVAVLDLSAEACQE
                     TVEAVEAAGSEAIAIACDVSSAQQVEKAFEEIAHRFGRIDILVNNAGLLRDNLSFKMS
                     EDDWDKVLDVHLKGSFLCSKAAQKYMVEQEYGKIVMTSSIVALGNKGQANYSAAKAGL
                     QALARTLALELGRFNINVNAVAPGWIETEMTKEAAERVGMTMEEMKDRFAKNIPLKRF
                     GRVDDIANVVAFLVSDDASYISGETLYVAGGPSSSVI"
     gene            complement(3226..4431)
                     /locus_tag="VFQ10_13545"
     CDS             complement(3226..4431)
                     /locus_tag="VFQ10_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9966413.1"
                     /translation="MATTAKQAEAPVHPGAGDFLNIDAHLSEEEIGVRERVRSFVEEK
                     IKPNIKGWYDEAVFPKEIVPRFADLGLLGMHLSGYGCAGKSAVEYGLACMELEAGDSG
                     LRTFVSVQGSLAMSAIHKFGSEQQKQEWLPPMARGEAIGCFGLTEPTAGSDPASMKTF
                     ASKEGSDWVLNGEKRWIGLATIADVAVVWARTDEEKNPVRGFLVPTNTEGFSATDITP
                     KLSMRASIQCDIRLQDVRLPEGAMLPEARGLSGPFACLGEARYGIIWGAVGAARDCYE
                     AALAYAIEREQFGRPIASFQLVQQKLANMLIEVGKGTLLALHIGRMKDAGTLRPEHIS
                     FGKLNNVREAIEIAREARTVFGGNGVTLDYPPMRHMSNLESVRTYEGTDEVHTLILGS
                     AITGIPAFR"
     gene            4538..>5614
                     /locus_tag="VFQ10_13550"
     CDS             4538..>5614
                     /locus_tag="VFQ10_13550"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET9966414.1"
                     /translation="MDEARKVPFEERERRAMLPLEGVKVLDLSRVLAGPYATMVLGDL
                     GADVLKVEHPERGDDTRHWGPPFAGGESAYFLSVNRNKRSIGVDLKDKEGLERVKTLA
                     AGADVLIENWRRGALEKLGLGYKALKEENPDLIYCSITGFGPGPDEDRPGYDFLVQAR
                     GGVMGITGQPGGEPTKVGVAISDIVCGLFASNAILAALHRRSATGEGTRIEVPLFEST
                     LGWLANRGQEYLLSGEDTGLIGNAHPSIVPYQTFHASDKPLVVAVGNNTQFANLCRAI
                     GRPELAEDVRYATNPGRVSNREALISELQQEFGKRSADEWAEEIRAAGVPSGPVNTLA
                     DVFADEHVLGSGMLQDLDHPSAGLL"
BASE COUNT         1068 a         1720 c         1714 g         1112 t
ORIGIN      
        1 gaggtccatg taggcgtcga tggactgctc gacgagagtc tggtaggcgt cgcgctggtc
       61 ctcggcgcgc tcgaagagct cgcccacaac actgcggttg ctctcagcct ggtggcggag
      121 ctccttgatg gtcccgtcca ctatgccctg cgcgaacctg acgtttcgct cctgtaggcc
      181 caccgcgtga tccacaaccg tctggtagga atcccgcgta ctctcagcga gcctctctac
      241 agccctgttg gtcttctgcg tagtggccat ctttatctcc tttctcgcgt aagacgcgaa
      301 ctactcgcca ctctcttctt tgtccttcaa cggcctgccc gtgtacagcc ggaagaagtt
      361 gtccatggtg tgctggtact gctcaagcga cttggcccag aagtcgaggt atgaccttcc
      421 agcgtccgtg atggtgtaca tcctgcgcgc aggcccgccc ctcgaggtct cccaactgga
      481 ctcgacgatg ccctctttct ccatctgccg gagcgttctg tacagcgttc cagggttcat
      541 cgcctcgaac ccgaaggccg aagcccgctc catgagctcg tacccgtatg agttccactc
      601 ccgtagagag agcaggatca caggcaccaa ccaattcctc ggccgcgcct caaccccacg
      661 gaactctcgc tccctgcctc tcgtctcgcg ctcctgctct tcgttgttca taactctctc
      721 ttccataatc tatgtgcatt ttacatatat atattgcact gtcaaggctc tgacgggcca
      781 ctcactctga atataccttt catcatcgag aacgccgacc cgacgttgga ccgcttctga
      841 agggctcatg gtggtgctcc gcgaatagct gtatgtcgaa cataagtaca cagtacatct
      901 tagcccaaat cggatcttct ttcggaacgg tcgtgtcgga ctggagcggg acgtggggag
      961 cctctcgacg tttttcctgg accggtttgc cggtcgggtc tcgtgtggct ctggggcgag
     1021 agtcaacacg ggcgccgatt ctatctctac cggcatcgtc ctccagctac gagcatcagg
     1081 cgcttttcgg aacggctttg gggaccagcg gtcgatgtgt gccatcttgg tgattcaacc
     1141 aatcttgcgc cggatttctc cagtcaataa actccgtctc acatgcgaac gggaaaagga
     1201 ggaaagcgtg ggcaccttga aagaagccgt tgtcgtcgtg acgggcgcga gcaggggtct
     1261 gggcaaagcc atagccgagg agttgtcggg cggtggagcc aaagtcgtcg tcaactactc
     1321 caggagcaag gaacctgccg aggagctagt gaaccagatc tcggagagcg gtggagaggc
     1381 tatcgcggtt caggctgatg tctccgacgc ggagcaggcc cagaagctca tagatcaggc
     1441 actggagagg ttcaaccggg tagacgtcct gatcaacaac gctgggatca acatagaccg
     1501 cactctcagg aagctctccg tggaggactg ggacaaggtc atacaggtag acctcaacag
     1561 cgccttctac acggtccacg cggtgttgcc gcacatgacg gaccagggcg ggggcaagat
     1621 catcaacatg agctccttcg tcggagaggc cggcaatata ggacaggcca actactcggc
     1681 ggccaaggcc gggctactcg gcttcaccaa gacggcagcc caggaactgg cccgcttcgg
     1741 gatcaccgtc aacgccatag ccccaggatt catagagacc gatatggtcg ccaacatccc
     1801 cgaggaagcc caacagaagc tccgcaaaca agtacccctg ggccgcttcg gaaaaccgga
     1861 agaggtagcc agggccgtgc gctaccttat agaagacggt gactacatca caggccagtc
     1921 aatcgatata aacggcggta tctacatcag gacgtaacga gagagagccc cgcccgcagg
     1981 cggggcttcc tcccacaacc ggccgtgtga gcctgcatac aggcagcccg caccggaacg
     2041 tatacgaacc cgccagagag ccagtggata ctgtgtccag agttgtacat atctgttctg
     2101 gacacataag ttcgtctcgt atacaattaa ctgccatgta acaaagacga caccctcctt
     2161 gggtgtagac agtagtgtca cctaacagag agaggtggac ctttgaggct tgatggacgt
     2221 acggcggttg taacgggggc tgcgcgcggc attggggcgg ctgaggccat ccggctggcg
     2281 caggaggggg cgaacgtagc ggtcctggat ctatcggctg aggcctgcca ggagacggtg
     2341 gaggccgtcg aggctgcggg gtccgaggcg attgcgatcg cctgcgacgt atcgagtgcg
     2401 cagcaggtcg agaaggcgtt cgaggagatc gcccaccgct ttgggcggat cgacattctc
     2461 gtcaacaacg ccgggctgtt gcgtgacaat ctctcgttca agatgagcga ggacgactgg
     2521 gacaaggtgc tcgacgtgca cctcaagggc agcttcctct gttccaaagc tgcgcagaag
     2581 tacatggtcg agcaggagta cggcaagatc gtgatgacca gctccatcgt ggctctgggg
     2641 aacaaggggc aggccaatta ctctgcggcc aaggccggcc ttcaggcact cgcgcggacg
     2701 ctggcgctcg agctcgggcg tttcaacatc aacgtgaacg ctgtggctcc gggttggatc
     2761 gagaccgaga tgaccaagga agcggccgag cgggtgggga tgaccatgga ggagatgaag
     2821 gaccgcttcg ccaagaacat ccccctcaag cgcttcggta gggtggacga cattgccaac
     2881 gtcgtcgcct tcctcgtctc cgacgacgcc tcctatatca gtggggagac gctttacgtt
     2941 gctggtggtc cttccagttc ggtgatttag agcatttcag caagtcggca tttcagcacg
     3001 gcgctacgcg ccttgtcagc acgcttcggc ctgcggcttc gctcgtcagc atttcagctt
     3061 tttccgttcc tgcctcgcct tgccgcgcat gaggaatcgc tgcatgtggg agtgggttcg
     3121 gcgcgcgtgg cgggctgaca agcgaaggcg aagcccgagc gtgctgaagt gctgacttgc
     3181 tgactagcgt ttacaaagag gtcgatgacc tctttgtaaa ctgcttcagc ggaacgctgg
     3241 gatgcctgtg atagcgcttc cgaggatcag ggtgtgcacc tcgtccgtac cctcgtaggt
     3301 ccttacgctc tcgaggttgc tcatgtggcg cattggagga tagtcgagcg tgacaccgtt
     3361 gccgccgaat acggtgcggg cttcgcgcgc tatctctatg gcctccctga cgttgtttag
     3421 tttgccgaag cttatgtgct cgggcctgag cgtgcccgcg tccttcatcc tgcctatatg
     3481 gagcgcgagg agcgtcccct tgccgacctc gatcagcatg ttcgcgagct tctgctggac
     3541 gagctggaag gaggctatgg gccgtccgaa ctgctcccgc tcgatagcgt aagcaagcgc
     3601 ggcctcgtag cagtctcgcg ccgcccccac cgctccccag atgatgccgt acctggcctc
     3661 gcccaggcag gcgaatggtc cgctgaggcc cctggcttcg ggcagcatgg cgccctcagg
     3721 gaggcggacg tcttggagtc ggatgtcgca ctggatcgaa gcccgcatgg agagcttggg
     3781 cgttatatct gtggccgaga agccttccgt attagtaggg acgaggaagc ctctgacggg
     3841 gttcttctct tcgtcagtgc gcgcccagac tacggcgacg tcggcgatgg tggccagccc
     3901 gatccaacgc ttctccccgt tcaagaccca gtcggagcct tccttgctgg cgaaggtctt
     3961 catgctcgct gggtctgagc ccgctgtcgg ctcggtgagg ccgaagcagc cgatggcctc
     4021 tcccctcgcc atcggcggca gccactcctg tttctgctgc tcggagccga atttgtggat
     4081 cgcgctcatg gcgagcgagc cctgcacgct cacgaaggtc ctcaggcccg agtcgcctgc
     4141 ctcgagctcc atgcaagcca ggccgtactc gacggcgctc ttgcccgcac acccgtagcc
     4201 ggagaggtgc atccctagca ggcctagatc ggcgaacctg ggtacgatct ccttcgggaa
     4261 gacggcctcg tcgtaccacc cctttatgtt cggtttgatc ttctcttcta caaaggaacg
     4321 gaccctctcc ctgacgccta tctcctcttc ggagaggtgc gcgtcgatgt tcagaaagtc
     4381 gcccgccccg ggatggaccg gagcctccgc ctgcttcgcg gtcgtagcca tcgtttctcc
     4441 tcgcgtagac agttcgtctc ctggtccgtc gattatccca catcgcccga ctgccccgtg
     4501 attggcctgg ggatgccggc acgggtagac tgacagcatg gacgaagcac gcaaggtccc
     4561 cttcgaggag cgagagcgta gggctatgct accgctagaa ggagtgaagg tcctggatct
     4621 ctcgcgggtt ctggccgggc cttacgcgac gatggtgctc ggtgacctcg gcgccgacgt
     4681 cctgaaggtc gagcatccgg agcgtgggga cgacacccgc cactggggtc cgccattcgc
     4741 cggcggcgag tcggcctact tcctctccgt caaccggaac aagcgttcga tcggggtcga
     4801 cctgaaggac aaagaagggc tggagcgggt caagacgctc gcggcagggg cagacgtctt
     4861 gatcgaaaac tggaggcgcg gcgcactcga aaagctcggc ctcggctaca aagctttgaa
     4921 ggaggagaac cccgatctca tctactgctc catcacgggc ttcgggcctg gcccggacga
     4981 ggaccgtccc ggctacgact tcctggtgca ggcgaggggc ggcgtcatgg gtatcacggg
     5041 ccaacccggc ggcgagccga cgaaggtggg cgtcgcgatc tcggacatag tgtgcggcct
     5101 gttcgcgtcc aacgccatac tggcagccct ccaccggcgc agcgcgaccg gagagggcac
     5161 gcgcatcgag gtgccgctct tcgagtccac cctcggctgg ctcgccaacc gcgggcagga
     5221 gtatctcctc agcggcgaag acaccggtct cataggcaac gcccatccct ccatcgtccc
     5281 ctaccagacc ttccacgcgt cggacaaacc cctcgtcgtg gccgtcggta acaacaccca
     5341 atttgccaac ctgtgcagag ctataggccg tcccgagctg gccgaggatg tgcgctacgc
     5401 gaccaacccc ggccgtgtga gtaacaggga ggctctgatc tcggagctgc aacaagagtt
     5461 cggcaagcgg agtgcggacg agtgggccga ggagatccgg gccgccggtg ttccgagcgg
     5521 acccgtcaac acgctcgccg acgtctttgc tgacgagcac gtcctcggct caggcatgct
     5581 acaggacctg gatcatccct cggccggact cctc
//
LOCUS       DASTKM010000337         7702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_328348, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000337 DASTKM010000000
VERSION     DASTKM010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328348"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VFQ10_13555"
     CDS             complement(<1..1262)
                     /locus_tag="VFQ10_13555"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9966415.1"
                     /translation="MAANEALRAMLEARSVAVVGASPRFETPGNYMMKQLIIGGFSGE
                     VAAVNPKYDEVEGVACFAGLDELPFVPDLVLLGVGNRRLEEQMEEAARCGARGVVVFA
                     SALENPPGDPPLTERLRSIAAEAGMVVCGANCMGFADVERGLRALAFEERADLEPGNV
                     AWISHSGSAFSALLHAERGIRFNLAVSTGQELTTTMADYMLYALERESTRVISLFLET
                     VRDPERFKLALATAAQRDVPVVALKVGRTHTSSRLVTAHSGALAGDDAAYEALFDAYG
                     VARVGTLNEMADLVELLGAGRRAAPGGLAAILDSGGERAHLVDAAADLEVPLARPSEE
                     TLSDVAARLEPGLPAVNPLDAWGTDKDPYNIFVDCSRHLADDPDTGAFAYVVDLHSDR
                     AERGHAWAAQRVWASTDKPFAVVCGVTSA"
     gene            complement(1267..2901)
                     /locus_tag="VFQ10_13560"
     CDS             complement(1267..2901)
                     /locus_tag="VFQ10_13560"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9966416.1"
                     /translation="MSGRYEAIVIGGGHNGLAAGAYLAHDGLRTVVLEARHKVGGAAT
                     TDAPWEEAPEFNVTTYSYVMSLMPRRVIDELRLEHHGYKVYPMGPSYYAFPDGRSLTM
                     TGVDAKKDYEALARFSRKDAEAYGEWRKWLGELAAIMEPLLLTTPPRIGSRHPLDILD
                     QLKLAWRMRGLDVARVGELTRLMTMSAWDLLDDWFESPQIKGAMAVDGIIGTWAGPAT
                     PGTAYVLMHHEIGDAGLGLSSWGYPRGGMGAVSDALRSSAEEFGAEVRTNAPVERILQ
                     RDGRVRGVVLEGGEELEADVVVAATHPRITFLEQLRREDLPDDFVGNIERWNSRSGVV
                     KINLALSELPDFSADPGPGDHLGGAIELAHSIDYIEEAFQHARRGEPARRPFSDGVIP
                     TVFDRTLCPEGYHIMSLFTQWVPHEWSEEPHREELEAYADRVIDGYSELAPNLKSAIL
                     HRQVLGPYDMEQDIGLIGGNIFHGELSAEQLFHMRPAPGYADYRTPIRGLYQASSATH
                     AGGGVCAIPAYNCVREIRRDRRRERMKERLTLRNRG"
     gene            3091..3759
                     /locus_tag="VFQ10_13565"
     CDS             3091..3759
                     /locus_tag="VFQ10_13565"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9966417.1"
                     /translation="MGARPKLTQKRRTQMLEAAMEVIADRGLCDTRIADVADRAGLSP
                     ALVVYYFGSKEKLLTEALAYAEDLFYIEAFRELTGIEGASERMVRLIELACPPVVRRE
                     ADEYWTLWVELWSRALRNDEAARKREALERRWRNTIAEVVREGQRSGEFGPCDPERFA
                     TYLSALMDGLALQVLFGDPAVNGELVYEMCLDAAAGELGFEPDASKRGGEEREAEEEL
                     AAEG"
     gene            3806..5071
                     /locus_tag="VFQ10_13570"
     CDS             3806..5071
                     /locus_tag="VFQ10_13570"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9966418.1"
                     /translation="MAFHPRERSPWRRRMSRREALRLMAGTAAAGGLLVGCGPGGGSS
                     SSASRVVIGTREDPVKQPLFDDNPMIESGLEPEKGPLRLYNWADYIYTRVLKDFEKEF
                     GVQVELTTFYNLEEATRKLRTGKVSFDVFVPTAEIIPKFVAGKLLQPLNHDYLPNLKK
                     NVWPMLASPYYDKGSRYTVPYTVYLTGIGWRTDKVAADVAAMENPWEAFWDPEYKEIA
                     GLYDDYRETIGVGLYRDGITDINGANPDDLKAAQNNLIELTDLVDIRYTIDGAYSKLP
                     EGVFGIHHAWSGDMVYAPYYMPKGDDPSVLRYVWPPKAQGGAGGYVSNDNFAVLKDAE
                     NPVLAHMFLNYMLDDKVAIKNFGWNGYQPPINTLEPNKLVEDGTIVKNLETTVIVQSD
                     FETGQTPQQLTPEEDERWLQAWSRVQQGG"
     gene            5078..5998
                     /locus_tag="VFQ10_13575"
     CDS             5078..5998
                     /locus_tag="VFQ10_13575"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9966419.1"
                     /translation="MSKADDERGMVGERRLLWPSFALPGVVWLLVLFVTPLYAVLAMA
                     MGVTDPIFGDSLPVWNPLRWDPSTFREVLGELFTGQLGVVFLRTLLYVAVATALSLLI
                     GYPVAYYLARHAGRYRVLLLMLVIAPFWINYLMRMLAWVNLLTTDGYVNQILMFVGTL
                     DAPREWLVGKHETVILGLVYGYVPFLILPLFAALDRIDQRVIEAARDLGASPAQAFFR
                     VTLPLSSQGVLAGCVIIMLPMFGDYYTATLLSGSPKTNMIAKLIEQNINASAGGSTGA
                     ALTIVLMVFVGLLMTYYLYSVARATREART"
     gene            5995..6915
                     /locus_tag="VFQ10_13580"
     CDS             5995..6915
                     /locus_tag="VFQ10_13580"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9966420.1"
                     /translation="MSAQANTTSGKALLARLQTWWSNPWGKPRFLVLFTWTYVLWSLV
                     PVAIAVLFGFNAGRSRSTWQGFSLRWYYQDPDLSVLHDPSLRDALLQSLKLAGLTMLI
                     ATPLGVALAIGLARWSGWGSRPANFLMLFPLVTPEIVMGVALFLVFVYLFGFIQLGTQ
                     AQLLGHVTFTVSYVVIIVRGRLFAVGREYEEAAMDLGASQWQAMRMVLLPMLTPAIWA
                     SLMIAFAISIDDFVITAFLMGDQSSATIPVKLYSAARAAPSPALNALASILLFASMLA
                     ITIAILVMRRSSKREGAEGSAVEDFARLDL"
     gene            complement(6967..>7702)
                     /locus_tag="VFQ10_13585"
     CDS             complement(6967..>7702)
                     /locus_tag="VFQ10_13585"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9966421.1"
                     /translation="GQQQRVALARALILNPSVLLLDEPLGALDAKLRKALQIELKALQ
                     EQVGVTFVYVTHDQEEALTMADRLAVMNEGRIEQVGSPREVYEEPASAYVADFLGVSN
                     MLDAQAIGTDSDGRCRVRVGDSELFASRGHTSARGPVKVVVRPERVRVEAPGETGENR
                     LPGKIERVVYAGAISQLVVTLNSGTPIRCMLANDGAGSSFDRGTLVSLYLPCEALRVL
                     RTETTAPNEEKQAISSQLSAVSSKKS"
BASE COUNT         1340 a         2464 c         2480 g         1418 t
ORIGIN      
        1 gcgctcgtta cgccacagac caccgcgaac ggtttgtcgg tcgaggccca gacccgctgg
       61 gcggcccagg cgtgaccccg ctcggcgcgg tcgctgtgca ggtccacaac ataggcgaac
      121 gcccccgtgt cggggtcatc ggccagatgc ctggagcagt ccacaaagat attgtaggga
      181 tctttgtccg taccccaggc gtcaagtggg ttgacggccg gcaggcccgg ctccagccgc
      241 gcggcgacgt cgctgagggt ctcttcggaa ggcctggcga gggggacttc gaggtcagcc
      301 gcggcgtcga ccaggtgcgc ccgctctcca ccagaatcga ggatggcggc caggcctcct
      361 ggcgcggcgc ggcgacctgc cccgaggagc tctaccaggt cggccatctc gttgagtgtc
      421 ccgactcggg ccacaccgta cgcgtcgaac agggcttcgt aggcggcatc gtccccggcc
      481 agcgcgccgg agtgggccgt gaccagccgg ctggaggtgt gcgtcctacc gaccttcagc
      541 gccacgacag gcacgtcgcg ctgggcggcg gtcgccagcg caagcttgaa tctctccggg
      601 tccctgactg tctccaggaa caacgagatc acccgtgtcg actcacgttc gagagcgtag
      661 agcatgtagt cggccatggt ggtcgtcaac tcctggcccg tggagacggc gaggttgaag
      721 cgtatgcccc tttccgcatg cagcagcgcc gagaaggcgg agccggagtg ggagatccag
      781 gccacgttcc ctggttccag atccgcgcgc tcctcgaagg ccagggcgcg cagcccgcgc
      841 tcgacgtccg caaaccccat gcagttggcg ccgcagacga ccataccagc ctctgccgcg
      901 atgctcctga gacgctcggt gagcggaggg tcgccgggcg ggttctcgag cgcgctggcg
      961 aacacgacga cgccccgcgc gccgcagcgg gcagcctcct ccatctgctc ctcgagccgc
     1021 ctgttgccga caccaagtag caccagatcg ggaacgaaag gcagctcgtc gagccccgcg
     1081 aaacaggcga cgccctccac ctcgtcgtac ttggggttga ccgcagccac ctcaccggag
     1141 aagccgccga tgatgagctg tttcatcatg tagttgcccg gcgtttcgaa ccttgggctc
     1201 gcgccgacca cagcgaccga acgcgcctcc agcatggcgc gcagggcctc gttcgccgcc
     1261 accgtgttat ccgcggttgc gcagggtcag gcgttccttc atgcgctccc tgcgccggtc
     1321 gcgccggatc tcccgcacgc agttgtaagc cgggatggcg cagacgccac caccggcgtg
     1381 cgtggctgag gaggcctggt agaggcccct gatgggggtc cggtagtctg cgtagcctgg
     1441 ggccggccgc atgtggaaga gctgctcggc cgagagctcg ccgtggaaga tgttgccccc
     1501 gatgagcccg atatcctgct ccatgtcata gggcccgagc acctggcgat gtaggatggc
     1561 gctcttcagg ttcggcgcca gttcgctgta gccgtcgatc acacggtccg cgtacgcctc
     1621 gagctcttcc ctgtggggct cctccgacca ttcgtgcggg acccactgcg taaagagcga
     1681 cattatgtgg tacccctcgg ggcagagcgt gcggtcgaac accgtgggta tgacgccgtc
     1741 ggagaaaggt ctgcgcgccg gttccccgcg ccgcgcgtgc tggaaagcct cctcgatata
     1801 gtcgatggag tgggcaagct ctatggcccc gccaaggtgg tctcccgggc cagggtcggc
     1861 gctgaagtct ggtagctccg agagggcgag gttgatcttt acgaccccgc tgcgggagtt
     1921 ccaccgctcg atgttgccga cgaagtcatc gggcaagtct tcccttctga gctgctcgag
     1981 gaacgtgatc ctcgggtgcg tggccgccac aacgacgtcg gcctccagct cctcaccacc
     2041 ctcgagcact acgcccctta cacgcccgtc cctttgcaaa atacgctcga ctggcgcgtt
     2101 ggtccttacc tccgcgccga actcctccgc cgaactcctg agggcgtcgg agacggcccc
     2161 catacctccc ctcggatatc cccaggagct gaggccgagg cctgcgtccc cgatctcgtg
     2221 gtgcatcaac acgtacgccg tcccaggggt cgcaggaccg gcccacgtcc cgatgatgcc
     2281 gtccaccgcc atcgcaccct ttatctgcgg cgactcgaac cagtcgtcga gcagatccca
     2341 ggcgctcatc gtcatgagcc tggtgagctc ccccacccgc gccacgtcaa gacccctcat
     2401 ccgccacgcc agcttcagct gatccaggat gtcaagggga tgcctggacc ctatcctggg
     2461 cggcgtcgtc agcagcaagg gctccatgat cgccgccagc tctccaagcc acttccgcca
     2521 ctccccgtac gcctccgcgt ccttgcgcga gaaccgcgcg agcgcctcgt agtctttctt
     2581 ggcgtctacc cctgtcatgg tcagggaccg cccatcaggg aacgcgtagt aagaaggtcc
     2641 catcggatag accttgtacc cgtgatgctc caggcgcaac tcgtcgatga cgcgccgggg
     2701 catcaggctc atcacgtacg agtacgtggt gacgttgaac tcaggggctt cctcccaggg
     2761 cgcgtcggtg gtggccgccc caccgacctt gtgacgggcc tcgagcacca ccgtcctcaa
     2821 cccgtcgtgg gccagataag cccccgcggc caacccgttg tgccccccgc cgatgactat
     2881 ggcctcgtaa cggccgctca tcaccctctc cttccgcaag cctgcgatca atactaaacc
     2941 atcattcaat gtagcatccc gcgttcgcgg tggcaagatg attcttttcg atcacggatc
     3001 acggactatt gtggactgga gtgggggctt gtgctatcgt tgtaaattaa atagtcgttc
     3061 acaagttcag atgtagagtg tgggagggtg atgggagcga ggcccaagtt gacgcagaag
     3121 cgccgcacgc agatgctgga ggcggccatg gaggtgatcg cggatcgggg tctgtgtgac
     3181 acgcgcatag ccgacgtggc ggacagggcg ggcttgagtc cggcgctggt ggtgtactac
     3241 ttcgggtcca aggagaagct tctgaccgag gcactcgctt acgcggagga ccttttctac
     3301 atagaggctt tcagggagct gacgggcatc gagggtgcat cggagcgtat ggtgcggttg
     3361 atagagctgg cgtgtccgcc tgtagttagg cgcgaggccg acgagtactg gacgctgtgg
     3421 gtcgagttgt ggtcgcgggc cttacgcaac gacgaggcgg cccgcaagcg cgaggcgctg
     3481 gagcgccgct ggcgcaacac catagctgag gtggtaaggg agggccagcg gtccggcgag
     3541 ttcgggccgt gcgaccccga gcgcttcgcg acttacctct ccgcgctaat ggacggcctc
     3601 gcgctccagg tattgttcgg cgaccctgcg gtgaacggag aactggtcta cgagatgtgt
     3661 ctggacgcgg cggccgggga gttgggattc gagcctgacg cgtcaaagcg gggtggtgag
     3721 gaaagagagg ccgaggagga gctcgcggcc gaggggtagg gacggggctg tctgggccgg
     3781 acgccccaaa aggaggttga tagaggtggc attccacccg agggaaagat ctccctggcg
     3841 gcggcgcatg tcgcgtcgcg aggcgctgag gctgatggcg gggacggctg cggcgggcgg
     3901 actccttgtt ggttgcggtc cggggggcgg ctcctcgtcg tcggcttcgc gcgtggtcat
     3961 cggcaccagg gaagaccctg tcaagcagcc ccttttcgac gacaacccga tgatcgagtc
     4021 tgggctggag cccgaaaagg ggccgttgcg cctgtacaac tgggcggact acatctatac
     4081 gcgcgtgctc aaggacttcg agaaggagtt cggtgtccag gtcgagctca cgactttcta
     4141 caacttagag gaggccaccc ggaaactgag aactggcaag gtcagcttcg acgtcttcgt
     4201 ccccacggcc gagatcatcc cgaagttcgt ggcggggaag ttgctgcaac ccttgaacca
     4261 cgactacctg ccgaacctga agaagaacgt ctggccgatg ctggcgagcc catactacga
     4321 caaagggtcg cgttacacgg tcccctacac cgtctacctg accgggatcg gctggcgaac
     4381 agataaggtc gccgccgacg tggcggccat ggaaaacccc tgggaggcgt tctgggatcc
     4441 ggagtacaag gaaatagcgg ggctgtacga cgactatagg gagacaatag gggtcgggtt
     4501 gtacagggac ggcatcaccg acatcaatgg tgccaacccg gacgatctga aggctgccca
     4561 gaacaacctt atcgagctga cggacctggt agacatccgg tacaccatcg acggtgcata
     4621 ctccaagctg ccggaaggcg tctttggcat ccaccatgca tggtcaggcg acatggtcta
     4681 cgcgccctac tacatgccaa agggtgacga cccttcggtt ctgcgctacg tgtggccccc
     4741 gaaggcgcaa ggcggggcgg gaggctacgt gtccaacgat aatttcgccg tactcaaaga
     4801 cgcggagaac ccggtcctgg cccacatgtt cctcaactac atgctcgacg acaaggtggc
     4861 gatcaagaac ttcggctgga acggctacca gccgcccata aacacgctag agccgaacaa
     4921 gctcgtcgag gacgggacta tcgtcaagaa cctcgaaacg acggtcatcg tgcaaagcga
     4981 cttcgagact ggccagacac cccagcagct gacgcccgag gaggatgagc gatggctcca
     5041 ggcatggtcg cgggtccagc agggcggcta gaggaccgtg agcaaggccg acgacgaaag
     5101 agggatggtc ggcgagagac gccttttgtg gccctccttc gccctgcccg gcgtagtctg
     5161 gctgctggtc ctcttcgtta cgcccctcta cgcggtcctc gcgatggcca tgggcgtcac
     5221 ggatcccatc ttcggggatt cgctgcccgt ctggaacccg ctcaggtggg acccgtcgac
     5281 gtttcgggaa gtcctcggtg agctgttcac cggccagctc ggtgtggttt tcttgcgcac
     5341 gctcctctac gtcgcagtgg ccaccgccct cagtctgctg atcggctacc ctgtagccta
     5401 ctatctcgcc cgccacgccg gccgctaccg ggttctgctc ttgatgctcg tcatcgcccc
     5461 gttctggatc aactacctga tgcggatgct ggcctgggtg aacttgctca ctacggacgg
     5521 ctacgtaaac cagatactaa tgttcgtcgg caccctcgac gcgccgcggg agtggcttgt
     5581 aggcaaacac gaaaccgtca tactaggcct cgtctacggc tacgtgcctt ttctgatcct
     5641 gccgctattc gccgcactcg ataggatcga ccagcgcgtc atagaagcgg cgagggacct
     5701 cggggcaagc ccggcacagg ctttctttcg cgtcacgttg ccgctctctt cgcagggggt
     5761 cctcgcgggg tgcgtgatca tcatgctgcc gatgttcggc gactactaca cggcgacgtt
     5821 gctgtcagga tccccgaaga cgaacatgat cgccaagctg atcgagcaga acataaacgc
     5881 cagcgccggg ggtagcacgg gcgccgccct gaccatcgtg ctgatggtgt tcgtcggcct
     5941 acttatgacc tactacctct acagcgtggc acgggcgacg agagaggccc ggacgtgagc
     6001 gcacaggcaa acactaccag cggcaaggcg ctccttgcgc gcctgcagac gtggtggtcg
     6061 aatccctggg gcaaaccacg cttcctggtg ctctttacct ggacctacgt gctctggtcg
     6121 ctcgtccccg tggcaatcgc cgtgctgttc gggttcaacg cgggacgctc tcgcagtacc
     6181 tggcagggct tctcgctccg ctggtactat caggacccag acctctccgt cttgcacgac
     6241 ccgtcgttgc gcgacgcgct gcttcagagc ctcaagctcg ctgggctgac catgctgatc
     6301 gccacgcctc ttggggtcgc gctcgccata ggactggcgc ggtggagcgg gtggggttcg
     6361 cgcccggcca acttcctgat gctcttcccg ctcgtcaccc cggagatcgt gatgggcgtg
     6421 gcgctgttcc tggttttcgt ctacctcttc ggcttcatac agctagggac ccaggcccaa
     6481 ctactcgggc acgtgacctt cacggtctcc tacgtagtga taatcgtgcg cggccgcctg
     6541 ttcgcggtcg gaagagagta cgaagaggcg gccatggatc tgggggcgtc gcagtggcag
     6601 gcgatgagga tggtgctgct gccgatgctc accccggcca tctgggccag cctcatgatc
     6661 gccttcgcca tctccataga cgacttcgtg ataaccgcgt tcttgatggg agaccagtcg
     6721 tcggctacga tcccggtcaa gctctacagc gccgcgcggg cggccccaag cccggcgctg
     6781 aacgccctgg cgagcatact cctcttcgcc tccatgctgg ctatcacgat cgccatcctg
     6841 gtgatgcgcc gttccagcaa aagggaaggc gcagaaggct ccgcggtgga agactttgcc
     6901 aggttggatc tgtaggggcg gttcgcgaac cgcccacata cgaaccgcgg cttcaggcaa
     6961 caggcttcag gatttcttcg agctgaccgc tgaaagctga ctgctgatag cttgtttctc
     7021 ctcgttcggc gcggtggttt cggtgcgtag gacgcgcagg gcttcgcatg gtaggtacag
     7081 cgacactagt gtcccccggt cgaaggacga tcctgccccg tcgttggcca gcatgcatcg
     7141 gatcggggtt ccgctgttga gggtgaccac gagttgggag atggcgccgg cgtagacgac
     7201 gcgttcgatc ttccccggca atcggttctc gccggtctct cctggggcct ccacgcgcac
     7261 gcgctcggga cgcacgacca ccttgacggg tcccctggcc gaggtgtggc cgcgggaggc
     7321 gaacagctcg gagtccccga ccctgacccg gcacctgccg tcagaatccg taccgatggc
     7381 ctgcgcgtcc agcatgttcg agacgccgag gaagtcggcg acgtaggcgg acgccggttc
     7441 ttcgtagact tcgcgtggtg agccgacctg ttcgatccgg ccctcgttca tcacagccag
     7501 gcggtcggcc atggtgaggg cctcttcctg gtcgtgcgta acgtagacga aggtgacccc
     7561 tacttgctcc tgcagggcct tcagctcgat ctggagtgct ttcctgagct tggcgtcgag
     7621 ggcgccgagc ggctcgtcca gaagcagaac ggatgggttg aggatcaaag cccgcgcgag
     7681 cgcgacccgc tgctgctgac cg
//
LOCUS       DASTKM010000338         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_148149, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000338 DASTKM010000000
VERSION     DASTKM010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148149"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..588
                     /locus_tag="VFQ10_13590"
     CDS             313..588
                     /locus_tag="VFQ10_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966422.1"
                     /translation="MDGEKVSICEMRMKGHLGGRPEWIDGLEIYNMENGEAVLSGQIV
                     DQAALRCVPAKAREPGIAAGRCDRVRSRQLRPRMDETEGERKCENRL"
     gene            726..1355
                     /locus_tag="VFQ10_13595"
     CDS             726..1355
                     /locus_tag="VFQ10_13595"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HET9966423.1"
                     /translation="MRHESRTIQPENAQSVRANLKMGAGELQVVGAADALMEADFSYN
                     VADWKPRVNYEVAGEAGELSVKQGSGGGVRLGGDARNEWNLRFNDEVPIDLRVQMGAG
                     ESDLDLDSLALTGLNLEIGAGRTTVDLTGDYEQDFDASIQGGVGQATVLLPSEVGVRA
                     RAEGGLGKINAEGLQREGDSYVNDSYGSSDVTVDVDVQGGVGEINLKLV"
     gene            1407..2177
                     /locus_tag="VFQ10_13600"
     CDS             1407..2177
                     /locus_tag="VFQ10_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966424.1"
                     /translation="MGVTPIQVAYPSVGDPHLRIALGACRFTARPGEGEVWVAGTYTD
                     PTDRRPLRIVEEEARVTITEAEPSFERIPAVFGGVPRYEVELGKMRPFSLTIETGASD
                     FDLDLGGIPLSRLMVRQGAGKLELGFPVANPEPMELLEISSGAAGIELENLANANFSE
                     MRLSGGAASYELDFGGVLSRNAEVSIETGLSAVELSVPSSTAASVVIETTLGSVDVGD
                     GFTKRDGAFSTEGALSEDGPVLRIRAGVRLGSLQLRTT"
     gene            2299..2496
                     /locus_tag="VFQ10_13605"
     CDS             2299..2496
                     /locus_tag="VFQ10_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966425.1"
                     /translation="MVEPPEPDENNLPNGDPDGEDEPEHTYGPSQEDARCLRWVVENS
                     SEIMKVVALDGALLSGAWRSR"
     gene            2493..>3157
                     /locus_tag="VFQ10_13610"
     CDS             2493..>3157
                     /locus_tag="VFQ10_13610"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9966426.1"
                     /translation="MSDVTKRREAEEKLRSQARELALFHRVRKALARELEVPGVLSRA
                     VEAVAETYGNTRLSAYLLEGGELVLQHQSGYHEALGRIPLTKGVCARAIRAGRPLLVE
                     DVSGDPDFLGFIEDVTSEICIPFFDEGEAVGFLFVESVDGVKLTENDLRVMVAVSEHV
                     DLAISRARLISRVRYSEERYRALTQNSSDLVTVMEITGIVRYQSPAIVRMLGYSQEEL
                     LGK"
BASE COUNT          654 a          886 c         1071 g          546 t
ORIGIN      
        1 gttgttgctg ggcattcccg gcgcgttgct ggccattcct gtggtcgaga tcatgcggat
       61 agtcgttacg gaagtactgg cctaccggcg taagagacag gaggcgaaca agcccgcagt
      121 ctcatccccg tcacagccct ctttatgaac acctgcccat tatcatgaat tacatacggg
      181 agaccctcgg aaacaagaga gccactcgtt cagggtccaa accgatgcgc agggccatgg
      241 atcggcgacc gatcctgatc cagaagcagc gaacgtcggg gcgcatcggt taaggaaacg
      301 gcatacgagc cgatggacgg agagaaagtg agcatctgcg aaatgcggat gaaaggacac
      361 ctcgggggcc ggccagagtg gattgacggg ttggagatct ataacatgga gaacggcgaa
      421 gccgtcctat cgggacagat cgtagaccag gctgcgctac gctgcgtgcc cgccaaggcg
      481 cgcgaacctg ggattgccgc aggccgctgc gaccgggttc ggtccagaca attgagaccc
      541 cggatggacg agacggaagg agagagaaag tgcgagaacc gtctttgagg attgccctgt
      601 cggttaagcc ggacggtgtc cttgtgagca aagcagatcg gataaagcgc ttcgtactcc
      661 tgttggtcgc cgcggtggct acggtgctat tcggagaggc ttgccaatcg caagaggtcg
      721 gagagatgcg gcacgagtcg cgcacgatcc agccggagaa cgcacagtcc gtacgcgcca
      781 acctcaagat gggagccggt gagctacagg ttgtcggcgc agccgatgcg ctgatggagg
      841 ccgacttctc ctacaacgta gccgactgga agcccagggt gaactacgag gtagccggcg
      901 aggcgggcga gctgagcgtc aagcaaggga gcggcggagg cgttcgcctc ggcggcgatg
      961 ctcgcaacga gtggaatctg cgcttcaacg acgaggtgcc gatcgatctc cgtgtacaga
     1021 tgggcgctgg ggagagcgat ctcgacctgg atagcctcgc gttgacgggg ctaaacctcg
     1081 agataggggc agggaggacc acggtggacc tcactgggga ttatgaacag gatttcgacg
     1141 ccagcataca gggcggggtt ggccaggcaa cggtgctgtt gcccagcgag gtgggcgtga
     1201 gggcgagggc cgagggcggc ctgggcaaga tcaacgccga gggactccag agggagggag
     1261 actcgtacgt gaacgattcc tacggcagct ccgacgtcac cgtggatgtc gatgtccagg
     1321 gtggcgtcgg agagatcaac ctgaagttag tctgaggtcg cctctgttcc cctacgttca
     1381 tgaagccgaa gagaaggagg attcgcatgg gagtgacgcc gatacaggtt gcatacccgt
     1441 ctgtgggcga cccgcacttg cgcatagccc tcggcgcctg ccgatttact gccaggccag
     1501 gcgagggtga ggtatgggtc gcaggaacct acaccgaccc gaccgataga agacccctca
     1561 ggatcgtcga agaagaggct cgcgttacga tcaccgaagc ggagccctcc ttcgaacgga
     1621 tcccggccgt cttcggaggc gtaccccgat acgaagtcga gttaggcaag atgcggcctt
     1681 tctccctgac gatcgaaacc ggcgccagcg acttcgatct ggacctgggg ggcataccct
     1741 tgagccggtt gatggttagg cagggcgctg gcaagctaga gctcggcttt ccagtagcca
     1801 accccgagcc catggagttg ctggagatct ccagcggggc cgccggaata gagctggaga
     1861 acctcgccaa cgccaacttc tccgagatgc gcctctccgg aggggcggcc agctacgagc
     1921 tcgatttcgg tggtgttctc tcgcggaacg ccgaggtgag tatagaaacg ggactctcag
     1981 cggtggagtt gtccgttccc tcctccactg cggcgagtgt ggtcatcgag actaccctgg
     2041 gcagcgtgga tgtgggcgac ggcttcacca aaagggatgg cgccttctcg actgaaggcg
     2101 ccctgagcga ggatggtccc gtcttgagaa ttcgagccgg agtgaggcta gggtcgctgc
     2161 agctcagaac cacgtagccc ctcgctaccg ggatgtacgc ctcggcaccg gggacctcgt
     2221 tggcggtacc catggccgac taacatccgc ctccggtata ctcttggtgc agtcatcgtc
     2281 agtgccggaa acactgtagt ggtggagcct ccagagccag atgagaacaa cctaccgaac
     2341 ggtgaccctg acggcgaaga cgagcccgag catacgtacg gcccgtccca ggaagacgcc
     2401 cggtgtttgc gctgggtcgt ggagaactct tcggagatca tgaaggtcgt cgctctcgac
     2461 ggggccctgc tgtcaggggc gtggcgatca cggtgagcga cgtcacgaag cgcagagagg
     2521 ccgaggagaa gcttcgcagc caggcgaggg aactggctct cttccatagg gttcgcaagg
     2581 ccctagcgcg cgagctggag gtgcctggcg tactatctag agcggttgaa gccgtagccg
     2641 agacctacgg caacactagg cttagcgcct acctgctgga aggcggggaa ctggtgctcc
     2701 agcaccagtc cgggtaccac gaggcgctcg ggcggatccc cctgacgaaa ggcgtctgcg
     2761 cgcgagccat acgcgccggg cggcccttgc tggtggaaga cgtgagcggc gaccctgact
     2821 tcctgggctt catagaggat gtgacctccg agatctgcat cccgttcttc gatgagggcg
     2881 aggctgtcgg cttcctcttc gtggagagcg tggacggggt gaagctgacg gagaacgacc
     2941 tgagggtgat ggtcgccgtg tccgagcatg tggacctggc catcagccgg gctcgcctca
     3001 tctccagggt gcgctacagc gaggaacgct acagggccct tacccagaac tcttcggacc
     3061 tcgtcacggt gatggagatc accggcatag tccgctacca gagcccggcc atagtgcgga
     3121 tgctcggcta ctctcaggaa gaactcctcg ggaagaa
//
LOCUS       DASTKM010000339         4221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1033832, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000339 DASTKM010000000
VERSION     DASTKM010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033832"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1040
                     /locus_tag="VFQ10_13615"
     CDS             204..1040
                     /locus_tag="VFQ10_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9966427.1"
                     /translation="MTDAAEATILVTGATDGLGRRVAQELAAMGATVLLHGRSPERLE
                     ATLEALRSQTGSEKANSYLADLSSLDAVRNLAKRILSGEDRLDVLVNNAGMIARERKE
                     SEDGYELTFQVNYLSHFLLTRLLLPLLKDSAPSRIVNVASAGQSPLDFSNLMLERGYD
                     AMKAYSQSKLAQVMFTFGLAEQLRGTGVTVNALHPASLMDTKMVQGTFGYIMSTVGEG
                     TEAVVRLAVSPEVDRITGSYFDGKREARANRQAYDPQARDRLWTLSEQLCGRLLEPDV
                     HR"
     gene            1432..2490
                     /locus_tag="VFQ10_13620"
     CDS             1432..2490
                     /locus_tag="VFQ10_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9966428.1"
                     /translation="MEYRRLGRSGLFVSTLTLGTMTFGGQGGFSKVGATDVAGARRQV
                     DMCLDAGVNLFDTANIYSSGESEEILGEAISGRRDDLILATKVRMSVGDGPNDTGLSR
                     YHIIRECEESLRRLATDYIDLYQVHEWDGLTPLEETLEALDTLVKSGKVRYVGSSNYS
                     GWQLMKALGTSERLGLQRYVSQQIHYTLQAREAEYELVPLAIDQECPILVWSPLAGGL
                     LSGKYRRDRDASEGRHVEGWDEPPVYDTDRLYDIVDVLVEIADGRGVSAAQVALAWLL
                     GRAGVASVIVGARTDEQLADNLRGAELELSEEERLRLDEVSALPLIYPFWHQAKTASD
                     RLGPADLSLLGPHVNEGS"
     gene            2725..>4221
                     /locus_tag="VFQ10_13625"
     CDS             2725..>4221
                     /locus_tag="VFQ10_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET9966429.1"
                     /translation="MGYLPIEDHGIIGDLHTAALVGTDGTIDWLCLPAFDSPSVFASI
                     LDDERGGHFSLRPAQYTRSQQLYLPGTNVLLTRFSAPEGVAEVLDFMPIGDDREPRHD
                     LIRDVRVIRGKVPIDIDCRPAFDYARQDHTIEFLRSGVIFRGAGMALNLASVVPLEEG
                     PRHSTVARFELKEGESASFALQQLDEGEEPREAPNRVECQELLQSTLNYWRRWISRST
                     YSGRWRDIVNRSALALKLMIYDPTGALVAAPTMALPQIMESAYNWDYRYTWLRDAAFA
                     MFVLIRIGFRDEAHKFMDWLQERSHGSDGLLQPIYGIDGRTVIDEVELHHLSGYRGSS
                     PVRLGNGNYDQLQLDLYGAVLDAAYLYNKYGAPLDYEDWQSLRSLLGWLSKNWHQPDA
                     GMWDLQSPREHFVSSKVLSWVALDRGIRLAHQRGLPADEEVWIGQRDAIYEEVMSRGW
                     NTKKESFVRHYGSEALDASLLLMPVVEFVGPTDPRWLATLDRLQKELTY"
BASE COUNT          858 a         1229 c         1311 g          823 t
ORIGIN      
        1 caaccacgcc cggacgtgcc gctggggcgt tcggttgctg gggaggtctg ccgttcgctg
       61 gctcgagcgt tagatgctgt ggttcagaag gagcactacg cagggcggac ttcctgaact
      121 ggcgtggcat ctcttggtct gtgccctagg attgactctg tgcaccaggc aaactgtgaa
      181 gccatggagt gaaaggaatg accatgactg acgcggcaga agcgacgata ctagtaacgg
      241 gtgccaccga cggcctgggc aggcgcgtcg cacaagagct cgcggcgatg ggcgctacgg
      301 tgctgctgca cggccgcagc ccggaacgcc tcgaagctac gcttgaggcg ttgaggagtc
      361 aaacgggcag cgagaaggca aactcctacc tggcggacct ctcctcgctc gacgcagtgc
      421 gcaacctggc gaaacgtatc ctctcgggcg aggatcgtct ggacgtcctc gtcaacaacg
      481 ctggcatgat cgcccgagag cgtaaggaga gcgaggacgg ctacgagctc accttccaag
      541 tcaactacct ctctcacttc ttgctcacga gattgctcct gccactactg aaggattcgg
      601 ctccgtcccg gatagtcaat gtcgcttctg cagggcaaag cccacttgac ttctcgaatc
      661 tcatgctgga gcggggctac gatgccatga aagcctactc acagagcaaa ctagcccagg
      721 tcatgttcac cttcggactg gccgagcagc tgcggggcac cggggtgacc gttaacgccc
      781 tgcacccggc ttccctgatg gataccaaga tggtccaggg caccttcggc tacatcatga
      841 gcacggtcgg agaggggacg gaggccgtcg ttcgcttggc cgtctcaccg gaagtcgata
      901 ggatcacggg cagctacttc gacgggaagc gggaggcacg cgcaaaccgg caggcctacg
      961 atccacaggc cagggatagg ctttggacct tgagcgagca actgtgcggg cgcttgctcg
     1021 aacccgatgt acaccggtga ttagcgcgtg ttctatccct gcttgggtgg gccactgcaa
     1081 tctaggatcg agtagagata tccttgtagt tggtgcttac cagcgcggcc agtcctgagc
     1141 aggccaatca ggttcagcag caacccctcc tgtaggagcc agtcggggac tatcagtata
     1201 cgcgcgagga gttgtttcga ggacccctgg agcatgcagc ataccttctt cctccctgaa
     1261 tgcttccgcc acttgaaggg cagttacggt ggctttgtat gatgtcgaga ttcggatcgc
     1321 cgcggagaag gcggtggagt ctgtgaaagg cggtccacga caccacaatg gagtgctcct
     1381 tatgctaggc tggcatgtat ctcgattgtt ccaccaggta gggaggattc tatggagtat
     1441 cgccgcctcg gacgctcggg tttgttcgtc tccaccttga ccctcggcac gatgacgttc
     1501 ggcggacagg ggggattcag caaggtcggc gcaacggacg tcgccggggc aaggaggcag
     1561 gtggacatgt gcctggacgc cggggtcaac ctcttcgata cggcaaacat ctactcctca
     1621 ggcgagtccg aggagatact gggggaggcg atctccggcc ggcgtgacga cctaatcctc
     1681 gcgacgaagg tccgcatgtc cgtgggagat ggcccgaacg acactggtct ctcgcgctac
     1741 cacataatcc gtgagtgtga ggaaagcctc aggagactcg ctaccgacta catcgacctc
     1801 taccaggtcc acgagtggga cggcctcacc ccactcgagg aaaccctcga ggcgctggac
     1861 accctcgtga agagcggcaa ggtacgctac gtgggctcct ccaactactc gggctggcag
     1921 ctgatgaagg cgctcggcac ctccgagagg ctcgggctgc agcgctacgt cagccagcag
     1981 atccactaca cgctgcaggc cagggaggcc gagtacgagt tggtgccgct cgccatagac
     2041 caggagtgtc ccatcctcgt gtggagcccg ctggccggtg gcctgctgtc gggcaagtac
     2101 cggcgcgaca gggacgcctc ggaggggaga cacgtggagg gctgggacga gcctcccgtc
     2161 tacgacaccg acaggctcta cgacatcgta gacgtgctcg tcgagatagc ggacggtcga
     2221 ggtgtatccg cggctcaggt agccctcgca tggctgctcg gacgagcggg tgtggcctct
     2281 gtgatagtgg gcgctcgtac cgacgaacag ctggccgata acctccgtgg cgccgagctg
     2341 gagctctccg aagaggagcg cctacgcctg gacgaggtga gcgcgctgcc gttgatctac
     2401 ccgttctggc accaggccaa gaccgcctcc gaccgtcttg gaccggctga cctgtcgctc
     2461 ctgggtccgc atgtaaacga gggatcgtag acttacaagc cgatacaccc gccgttgtag
     2521 gttgtgagga gcccgatagt cgtccgaagc tattttactg ctccgcaaca ccctccctta
     2581 ggagaggcgg aaatgataag ggacgatcgg gacgcgtacg agaagcgtcg aaacggtctt
     2641 gaacctgacg atggccttta ccgttcacgg tcttgtaggg caaaatgcac gccaagcagc
     2701 cggtccacta ctacgggaat gtacatgggc tacctcccta ttgaagatca cgggatcata
     2761 ggtgatcttc acactgctgc tctggtcggc acggacggga ctatagattg gctttgcctg
     2821 cccgctttcg actctcccag tgtcttcgct tctatcctgg atgacgagag gggtggccac
     2881 ttcagtctcc gtcctgcaca gtacactcgc agtcagcagc tctacctgcc tggtaccaat
     2941 gttctcctta cccgcttcag cgctcccgag ggcgtcgccg aggtactgga cttcatgccc
     3001 attggggatg atcgtgaacc acgccacgac ctgatccgcg acgtccgggt cataaggggt
     3061 aaggtaccaa tcgatatcga ttgtcgcccg gccttcgact acgctcgcca agatcacacc
     3121 atcgaattcc tcaggagcgg ggtgatcttc aggggggcgg gcatggcctt gaatcttgct
     3181 agtgtggtgc cactcgaaga gggcccgcgc catagtactg tcgcgcgatt tgagttgaag
     3241 gagggcgaga gtgctagttt cgccctccag cagctcgacg aaggcgagga gcccagggag
     3301 gcacccaatc gagttgagtg tcaggagttg ctgcaaagta cgctgaacta ctggcggcgg
     3361 tggatctcca ggagcaccta cagcggccgt tggcgcgaca tagtgaacag atctgccctg
     3421 gccctcaaac tcatgatcta cgaccccacc ggagcacttg ttgcggcccc cacaatggcg
     3481 cttccgcaga ttatggagag tgcgtacaac tgggactacc gctacacctg gctcagggac
     3541 gctgccttcg caatgttcgt actcatccgt atcggcttta gggacgaggc gcacaaattt
     3601 atggactggc tccaagagcg ctctcatggc tctgacggtc tgctccagcc gatctacggc
     3661 atagacggga ggactgtaat agatgaggta gagttgcacc acctctccgg ctatcgtggc
     3721 tctagcccgg tacggttagg taatgggaac tacgaccaat tacagctcga tctctacggc
     3781 gccgtcctgg acgcggccta cttgtacaac aaatacggtg cgcccctgga ttacgaagac
     3841 tggcagagcc tgcgaagcct ccttggttgg ctcagcaaga actggcacca gccggacgcg
     3901 gggatgtggg accttcaaag ccctcgcgaa cacttcgtat cgtcgaaggt gttgagttgg
     3961 gtagcgctcg atcgagggat acgattggcg catcagaggg gactacccgc tgacgaggaa
     4021 gtctggatcg gccaacggga tgctatctac gaagaggtca tgagcagggg ctggaatact
     4081 aagaaggaaa gcttcgtcag gcactacggc tccgaggctc tggacgcctc gctgctgctc
     4141 atgcccgtgg tcgagttcgt cggtcccaca gatccacgct ggctcgccac cctcgatcgt
     4201 ctgcagaagg agttgaccta t
//
LOCUS       DASTKM010000340         5994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_858006, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000340 DASTKM010000000
VERSION     DASTKM010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858006"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VFQ10_13630"
     CDS             <1..1285
                     /locus_tag="VFQ10_13630"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9966430.1"
                     /translation="DLEKRVAERTEQLKSAMAKQQQEAQERERIEQELRVARLIQHAL
                     LPKSAPELGSYQIAAYYRPAREVSGDFYDFLEIEDGRLGLVVGDASGKGIPAAMVMAN
                     TRSVLRTVAQGGDIAPGQVLEEANEILYPDIPPNMFVTCFYGVLDPSSDTFTYANAGH
                     DLPYLHRNGDAEELRARGMPLGLMPGMSYEEREVVVDAGESVLFYSDGLVEAHDPQGE
                     MFGFPRLRALVAEHDEEEHSLVDFLLEELYSFVAEGWEQEDDITLVTLGKIGGTLTAP
                     TSGARRMISELSMPSEPGNERQATEEVARAVSGLGLPQRTLERLKTAVAEATMNAMEH
                     GNRYDPDVPVKIQVWLLEESLLVRIIDRGGSPPPSPNATEVPDLEAKLEGMQTPRGWG
                     LFLIKNMVDEVRVSGNPDHHTVELVMLLKGEDDAS"
     gene            1275..1631
                     /locus_tag="VFQ10_13635"
     CDS             1275..1631
                     /locus_tag="VFQ10_13635"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9966431.1"
                     /translation="MPAKHLEASVRQERGGTVLDLRGEINDFAQEALNAAYAEAEAKD
                     PGAIILNFGGVDYIDSAGIVLIGNLLAKARASKRRLLAYGLSDHYVEIFDITRLSDFV
                     SIFPDEVSALSGASAS"
     gene            1631..1954
                     /locus_tag="VFQ10_13640"
     CDS             1631..1954
                     /locus_tag="VFQ10_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HET9966432.1"
                     /translation="MDGDGILVHATIPVDADPGEDHDFVIVDLPDIEQAATILHRGAR
                     DDVMPTIQALTRWIDTNGYRSAGCNRQLYIELGENRDNRVVTPPVPAPTTSSDMSSPI
                     RGVGD"
     gene            1976..2806
                     /locus_tag="VFQ10_13645"
     CDS             1976..2806
                     /locus_tag="VFQ10_13645"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9966433.1"
                     /translation="MKDHMVCRTHIPRPPLSDFVEVFWQYDGLDPSHARERCLPTGTM
                     EIVVNLRDDETRAYDRRSAYRPRKLRGPLVCGAHSGFFVIDTTGQASTIGVHFKPGGA
                     FPFLGPAGELRDALVPLESLWGAKAAELRDRLLDAATAEARFRVLEQTLVARATSLPG
                     RHPAVAFALERFESVPQRLKVSEVARRSGLSQRRFIRVFDEEVGLTPKLFCRIQRFQR
                     VLHLVENGRGAEWADVALGCGYFDQAHLINDFRAFSGVTPTAYLAGRSDHFNHLPLPD
                     "
     gene            2928..3611
                     /locus_tag="VFQ10_13650"
     CDS             2928..3611
                     /locus_tag="VFQ10_13650"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9966434.1"
                     /translation="MTGELTFFEIGVVDAAPARAFYGGLFGWRIEPGPSAGGYRISTP
                     TISGGMHGGDETLESRTDEPREESFGRFALCRDDQGSPFGLHQPPAAEGKGAREPAAR
                     RPTIDHVTLRVRDLPASRRFYEVALAPLSLGLEFEHEGLIAFGSGESGRLIIYASKRP
                     VAGFHVAFSAPSREAVNRFHAAALEAGGRDNGAPGLRPEYHEGYYGAYVFDPDGNNVE
                     AVHHTVPSG"
     gene            3747..4904
                     /locus_tag="VFQ10_13655"
     CDS             3747..4904
                     /locus_tag="VFQ10_13655"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HET9966435.1"
                     /translation="MNIQPFEVNIPQADLDDLHERLTRTRWPDELPGVGWSRGVPLGY
                     LKELTEYWQNGYDWRLWEANLNEFPQFTTSIDGQNIHFLHVRSPEPGALPLIISHGYP
                     SSVVEFLSIVGPLTDPVSHGGVAADAFHVVAPSIPGFGFSTPVRETGWEMGRTARAFA
                     ELMDRLGYKRYGAHGGDVGAGIVGMLAGLAGEHVAAVHTNSDPLAVMGALDYLPEGAV
                     RLAGLSEADREAVEETKAISEEGSGYLKLQSNRPQTIAYSLTDSPVGQLAWIFENFKE
                     WTDQANDLPEDAVDLDQLLTNVSIYWFTRTGASAARFLYETAHSTEWGAPSTAPQGWA
                     LFAAQPFVRAMMDPDHDNEHWSEFDRGGHFPAMEVPDLLVGDVRKFFRPFR"
     gene            5088..5734
                     /locus_tag="VFQ10_13660"
                     /pseudo
     CDS             5088..5734
                     /locus_tag="VFQ10_13660"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
     gene            complement(5807..5877)
                     /locus_tag="VFQ10_13665"
     tRNA            complement(5807..5877)
                     /locus_tag="VFQ10_13665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5822..5824),aa:Ser,seq:cga)
BASE COUNT         1101 a         1900 c         1951 g         1042 t
ORIGIN      
        1 ggacctcgaa aagagggttg cagagcgaac cgagcaactc aagagcgcca tggccaagca
       61 gcagcaggag gcccaggaac gagagcgcat cgaacaggag ctgcgggttg ctcgcctgat
      121 ccagcacgcc cttctgccga aatcggcacc cgagctcggg agttatcaga ttgcggccta
      181 ctaccggccc gcccgagagg tgagcggcga cttctacgac ttcctagaga tagaggacgg
      241 ccgactgggg ctcgtagtcg gggacgcctc cggcaagggg atccctgcgg ccatggtgat
      301 ggccaacacg cgcagcgtgc ttcggaccgt cgcccaagga ggggatatcg caccgggaca
      361 ggtgttggag gaggccaatg agatccttta tcctgacatt cctcccaaca tgttcgtcac
      421 ctgcttctat ggcgtcctcg acccctctag cgacaccttc acctacgcca acgccggcca
      481 cgacttgcct tacctacacc gtaacggtga cgccgaggaa ctgagagcca gagggatgcc
      541 cttggggcta atgccgggga tgagctacga ggagagggag gtagtggtcg atgctggcga
      601 gagcgtcctc ttctacagcg acgggctggt cgaggcccac gacccccaag gagagatgtt
      661 cggcttccct aggctgcggg cgctcgtggc ggagcacgac gaagaggagc actcgctggt
      721 ggacttcctc ttagaagagc tctactcctt cgttgcggag ggctgggagc aggaggatga
      781 cataaccctg gtgaccttgg gaaagatcgg aggaaccttg acggcaccca caagcggcgc
      841 acggcggatg atctccgagc tgagcatgcc cagcgagccc ggcaacgagc ggcaggcgac
      901 ggaagaggtg gccagggcgg tgagcgggct ggggttgcct cagaggaccc tggagagact
      961 caagactgct gtggccgagg cgacgatgaa cgccatggag cacggcaaca ggtatgatcc
     1021 ggacgtcccg gtcaagatcc aggtgtggct ccttgaggag agcctcttgg tgcgcatcat
     1081 cgaccggggt ggtagccctc caccgagccc gaacgccacg gaggtgccag acctggaggc
     1141 aaagctggag ggcatgcaga cgccgcgggg gtgggggctg ttcctgatca agaacatggt
     1201 cgacgaggtg cgcgtctcgg gaaacccgga ccatcacacc gtcgagctgg tcatgctctt
     1261 gaaaggtgag gacgatgcca gctaaacacc tcgaggcaag tgtgcgccag gagcggggtg
     1321 gcaccgtcct tgaccttcgc ggcgagatca acgatttcgc ccaggaagcc ctcaacgccg
     1381 cctacgctga ggcagaggcc aaggacccgg gggccatcat actcaacttc gggggggtag
     1441 actacatcga ctcggctggg atcgtcctga ttgggaacct gcttgcgaag gcgcgcgcct
     1501 cgaagcgtcg ccttctggcc tacggcctaa gcgaccatta cgtcgagatc tttgacatca
     1561 ctcgcctctc ggacttcgtg agcatcttcc cggacgaagt gagcgccctg agcggggcgt
     1621 ccgcgtcctg atggatggcg acgggatcct cgtccacgcc accattccgg tcgatgccga
     1681 tcccggtgag gaccacgact tcgtgattgt ggacctgccc gatatcgagc aggcggccac
     1741 gatcttgcac cgcggcgcga gggacgacgt catgccgacc atccaggcgc tgacgcgctg
     1801 gatcgacacg aacgggtacc gctcggccgg ctgcaaccgc caactgtaca tcgagctcgg
     1861 cgagaacaga gataataggg tggtgacgcc tccggttcct gctcctacga cgtcttcgga
     1921 tatgtcctct ccaatacgcg gggtgggaga ttgatcgttc tcacgtagaa tggctgtgaa
     1981 ggatcatatg gtctgccgca cccacatacc ccggccgccg ctctcggatt tcgtggaggt
     2041 gttctggcag tacgacggcc tcgatccctc gcacgcgcgg gaacgctgtc ttccgaccgg
     2101 tacgatggag atcgttgtca accttcgcga tgacgagacg cgagcgtacg accgacgaag
     2161 cgcctaccgg ccgcggaaac tccgcggccc gctcgtctgt ggcgcgcatt cggggttctt
     2221 cgtcatagac actaccggcc aagcttcgac gataggcgtg catttcaagc cgggcggcgc
     2281 cttcccattc ctcggacctg ccggggagtt gcgggacgcc ctcgtcccgc tcgagtcgtt
     2341 gtggggagcc aaggctgcgg agctccgcga tcgtttgctc gacgccgcca cggcggaggc
     2401 gaggttccgt gttctcgagc aaaccctcgt cgcgcgggca acaagcctcc cggggcgcca
     2461 tcctgccgta gcttttgcgc tggagcgttt cgagagcgtt ccccaacggc tgaaggtctc
     2521 ggaggtcgcg cggcggtccg gcctcagcca gaggcgcttc atccgggtct tcgacgaaga
     2581 ggtcgggctg acgcccaagc tcttctgccg catccaacgc ttccagcggg tgctacacct
     2641 cgtcgagaac gggcgggggg cggagtgggc agacgtcgcc ctggggtgcg gttacttcga
     2701 ccaggcccat ctcataaacg acttccgtgc cttctctggc gttacaccga cagcctacct
     2761 cgcgggcagg agcgatcact tcaatcacct tccgctcccg gattagggtc agaaacgggt
     2821 ggcgatccgg gtcaattttc tccaatagga tcgctctcct cccgagtagg atgagtttag
     2881 cgcttaggac aggcactcag acggaagcat ctacgaaagg agagcccatg accggtgagc
     2941 tgacgttttt tgagataggt gtcgtcgatg cggcgcccgc ccgcgccttc tacggcggat
     3001 tgttcggctg gcggattgag cccgggccgt ccgctggcgg ctaccggatc tctaccccga
     3061 cgatctcggg tgggatgcac gggggcgacg agacgctcga gagccgcacc gacgagccac
     3121 gggaggagtc cttcggacgc ttcgcgctct gccgcgacga tcagggctcc cccttcgggc
     3181 tccaccaacc gcccgcggcg gagggcaaag gggctcgaga gcccgccgcc cgcaggccga
     3241 cgatagacca cgtcaccctg cgggtgaggg atctgccggc cagcaggcgg ttctacgagg
     3301 tggccctggc tcccctgagc ctcggcctgg agttcgagca cgagggcctg atagccttcg
     3361 gaagcgggga gagcgggagg ctcatcatct acgcaagtaa gcggcccgtc gccgggttcc
     3421 atgtcgcttt ctccgctcct agccgggagg ccgtgaaccg tttccacgcc gcggcgctgg
     3481 aggcaggagg gcgcgataac ggcgcgccgg ggctgagacc cgagtaccac gaaggctact
     3541 acggcgccta cgtattcgac ccggacggca acaacgttga ggcggtccat cacaccgttc
     3601 cctccgggta gaacccatga tgaacagcgc acacgctaga tccctgtttc ttgacagtcg
     3661 cagccaatca ggaatgattc tgacttcctt tggcgatacg atcaagccat gatggagacc
     3721 aggagagaag gaggcaccag ttcgagatga acatacaacc tttcgaagtg aacatcccgc
     3781 aggccgatct cgacgatctg cacgaacgct tgacccgcac acgctggcct gatgagcttc
     3841 ccggtgtcgg atggagccgg ggcgtgccac tggggtacct gaaggagctg accgagtact
     3901 ggcagaacgg atacgattgg cgcctgtggg aggcgaacct caacgagttt ccccaattca
     3961 ccacctccat cgacggccag aacatacact tcctgcacgt ccgctcgccc gagcctggtg
     4021 cgctaccgct gatcataagc cacggatacc ccagttcggt ggtggagttc ctgagcatcg
     4081 tcggaccgct caccgatccg gtttcccatg ggggcgtcgc cgccgacgcc ttccacgtgg
     4141 tagcgccatc gatcccgggc ttcgggttct ccaccccggt ccgagagaca ggctgggaga
     4201 tgggccgcac cgcccgggcg tttgccgagc tgatggaccg cttgggatac aaacgctacg
     4261 gtgcccacgg aggtgacgtc ggcgcgggga tcgtcggaat gctcgccggc ctcgccggcg
     4321 agcacgtcgc ggcggtgcac accaactccg atccactcgc tgttatgggt gcgctcgact
     4381 atctgcccga aggtgcggtg cggctcgccg gcctctcgga agccgaccgg gaggccgtgg
     4441 aggaaacgaa ggcgatcagc gaggaggggt cgggatacct caagctccag tcgaaccgtc
     4501 cccagaccat agcctactcc ctcaccgact cgcccgttgg ccaactggca tggatcttcg
     4561 agaacttcaa ggagtggacc gatcaagcga atgaccttcc cgaagacgcg gtcgatctcg
     4621 atcagctgct caccaacgtc tcgatctact ggttcaccag aaccggagcc tcggctgccc
     4681 gcttcctgta cgagaccgca cactcgacgg agtggggtgc gccgagcacg gcgccgcagg
     4741 ggtgggcgct cttcgccgcc cagccgttcg tgcgggcgat gatggacccc gaccacgaca
     4801 acgagcactg gtcggagttc gataggggcg gccacttccc ggccatggag gtccccgatc
     4861 tgctcgtcgg cgacgtgcgc aagttcttcc gcccgttccg atgaagggcg aggtcctggg
     4921 tcggcgcgcg ctcaaccgcg ccctgctgag gcggcaactt ctgctgcgcc gccacgagat
     4981 gtcggccgcc gaagctatag agcacctggt cggtatgcag gcccaggagc cccgccgacc
     5041 cgtacatcgg cctgtggact cgcgtagaag gctttcgcca cgaggagttg tctgccctta
     5101 tctccgaccg gcgcgcggtg cgtgcttcca tgatgcgcgc gaccattcac ctgctcaccg
     5161 cccgcgacta cctggcgctg cgctccgtgg tgcagccggt cctcgaacgg gacgtcctcc
     5221 ccaaccccac ctacggtcgc gagcgtcttg aggggctgga cgtggacgcg ctcctggcgg
     5281 ccggtcgcgg gctgttgggc gagaggccgc gcatggcagc cgagctccgc cggctgctcg
     5341 gaccccggtg gccggaacgc gacccggcgg cgctggccta cgccgtccgc ggcctgtgcc
     5401 ggtggtctac gtcccgccgc gaggcatctg gggcaagagc gggcccgtag cgatgaccac
     5461 ggtcgaggcg tggctcggcc ggggcgtcga gtccgaccgc gaaccggacg ggatggtcct
     5521 gcgctacctc gccgcgttcg gcccggccgc catttcggat gtccggacct ggtcggggct
     5581 gaccggcctg cgggcggtcg tcgatcggct acggtcgcgg ttgcgcacct tccgtgacga
     5641 gagcggccgg gagcttttcg acgtgacagg cgcgccgctg cccgactacg acccaggtaa
     5701 catcaatctt tgggttgcag tacggttgca gtaaatcgtc ccggaatgcc ttcaggcctg
     5761 tctacaacct atcacgtttt acctgcaaat ccgtagaaaa agagagtgaa cccgagggga
     5821 ctcgaacccc tgacctccgc catgcgaggg cggctcgata gttttccaaa cgtttccaga
     5881 acttgcaaaa tacctgcaaa tcgccgaatt acagtgttga aacttttctc agcatttcag
     5941 gagatttacc agggttgctg cacggttgct gcacaactat ctgggctaac aagc
//
LOCUS       DASTKM010000341         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_682070, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000341 DASTKM010000000
VERSION     DASTKM010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682070"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..84
                     /gene="rpmJ"
                     /locus_tag="VFQ10_13670"
     CDS             <1..84
                     /gene="rpmJ"
                     /locus_tag="VFQ10_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET9966436.1"
                     /translation="CERCKVIRRRGVVRVICTNPRHKQRQG"
     gene            86..457
                     /gene="rpsM"
                     /locus_tag="VFQ10_13675"
     CDS             86..457
                     /gene="rpsM"
                     /locus_tag="VFQ10_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET9966437.1"
                     /translation="MARIAGVDLPREKRVEVGLTYIYGVGRSTARKICRDTEVDPDTK
                     VRDLAEGEVARVRTYIDQNVEVEGDLRREVNQNIRRLIDTGTYRGIRHRRGLPVRGQR
                     TKTNARQRKGPKPSIGGRKKR"
     gene            458..874
                     /gene="rpsK"
                     /locus_tag="VFQ10_13680"
     CDS             458..874
                     /gene="rpsK"
                     /locus_tag="VFQ10_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET9966438.1"
                     /translation="MGRQRQQRQRTRGASRARRRVRRNVTTGVVHIKSSFNNTIVSVS
                     DQEGNVIAWESAGSLGFKGSRKSTPFAAQMTAESVANKAMDQGVRRVDIEVKGHGSGR
                     DMAARTFQSMGIEVLSIKDVTGQPHNGCRPPKRRRG"
     gene            880..1482
                     /gene="rpsD"
                     /locus_tag="VFQ10_13685"
     CDS             880..1482
                     /gene="rpsD"
                     /locus_tag="VFQ10_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET9966439.1"
                     /translation="MARYTGPRGRRDRRAGVMLSSMRKNPLEKKPYPPGEHGRGRHRQ
                     TEFGLRLMEKQKARWYYGVSEKQFRRAYDRATRMQGVSGENLLRLMELRMDNVVYRMG
                     FSTSRPQARQLVVHGHFQLNGRKHNVPSAILKPGDVITVKEKSRDLEPIQNAVENVVA
                     VPPWLEADHDNFTGRLLHTPGRDEIDTPVEEQLIIEFYSR"
     gene            1517..>2419
                     /locus_tag="VFQ10_13690"
     CDS             1517..>2419
                     /locus_tag="VFQ10_13690"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET9966440.1"
                     /translation="MLDIAPPRFRVEEEEARRGTFVAEPLPRGLGHTLGNSLRRVMLS
                     GLTGAAVTKIRIEGVSHEFSTIEGVKEDVVDIILNIKSLKFKLEREEPIELTIAKDGP
                     GDVTAADIELKADVEVVDPEAYIASVSDGGHLDMRLTVERGQGYARAEQNKSEADPIG
                     VIAVDSLFSPVQRVNYTVAETRAGARADLDSLRMEVFTDGRIEPQEALQDASRQLMDF
                     LGLFTDGYQGTGVPDARQRGGRPVITDERPVEDLELTVRSYNCLKREGVDTIGQLATM
                     TEEELMNIRNLGMKSVDEIRSKLLE"
BASE COUNT          539 a          714 c          823 g          343 t
ORIGIN      
        1 tgcgagcggt gcaaggtcat aaggcgtcgc ggggtggtgc gtgtcatctg cacgaaccct
       61 cgccacaagc agaggcaggg ttagcatggc gcggattgca ggagtagacc tccccaggga
      121 gaagagggtc gaggtaggcc ttacctacat ctacggtgtt ggccgttcga cggcgaggaa
      181 gatctgccgc gataccgagg tcgatcctga cacgaaggtg cgggacctgg ccgagggaga
      241 ggtcgctagg gtcaggacct acatagacca gaacgtcgag gttgagggcg acctgaggcg
      301 cgaggtcaac cagaatatca ggcggctcat agacacagga acctaccgcg ggatcaggca
      361 ccgcaggggc ctgcccgtgc gcgggcagcg taccaagacg aacgcccgcc agcgcaaggg
      421 acccaagccc tcgataggcg ggaggaagaa gaggtagatg ggtaggcaga ggcaacagcg
      481 gcaacgcacg cgtggggcga gcagggcccg tcgcagggta cgcaggaacg taacgaccgg
      541 cgtggtccac ataaagagct ccttcaacaa caccatcgtc tccgtcagcg accaggaggg
      601 caatgtcatc gcctgggaat cggcgggatc cttgggtttc aaagggagcc gcaagagcac
      661 gcctttcgct gcccagatga cggccgagag cgtcgccaac aaggccatgg accagggcgt
      721 gaggcgtgtg gacatcgagg tcaagggcca cggctcgggt agggacatgg ccgcccgcac
      781 cttccagtcc atgggcatag aggtgctctc cataaaggac gtaacgggcc agccgcacaa
      841 cgggtgcagg ccgcccaaga ggcgcagggg gtagcaagta tggcgcgtta cacaggaccg
      901 aggggccgga gagaccggcg ggcaggggta atgctctcat ccatgcggaa gaacccgctg
      961 gagaagaagc cctacccgcc aggagagcac ggaaggggca ggcatcgcca gaccgagttc
     1021 gggctcaggc tcatggagaa gcagaaggcc cggtggtact acggggtctc cgagaagcag
     1081 ttcaggcggg cctacgacag ggccaccagg atgcagggcg tctcgggcga gaacctcctc
     1141 aggctcatgg agttgaggat ggacaacgtg gtttacagga tgggcttctc caccagccgc
     1201 ccgcaggcgc ggcagctcgt cgtgcacggg cacttccagc tcaacggccg caagcacaac
     1261 gtcccttcgg ccatactcaa gcctggcgac gtcatcacgg tcaaggagaa gagcagggac
     1321 ctggagccca tccagaacgc cgtcgagaac gtggtcgccg tcccgccctg gctggaggcc
     1381 gaccacgaca acttcacggg caggctcctg catacgccgg ggcgtgacga gatcgacacg
     1441 cccgtcgaag agcagctcat tatcgagttc tacagccggt agcgacaaga catttatagc
     1501 aggtgatact ttgacgatgt tggacatagc accaccccgt ttcagggtgg aagaagaaga
     1561 ggccaggcgc ggaaccttcg tcgccgagcc gttgccgcga ggactgggcc acaccctggg
     1621 caactcgctc aggcgcgtca tgctctcagg gctcacgggg gccgcggtca ccaagatccg
     1681 catagagggt gtctcgcacg agttctcgac gatcgaaggc gtcaaagagg acgtcgtcga
     1741 catcatcctc aacatcaaga gcttgaagtt caagctggag cgggaggagc ccatagagct
     1801 cactatcgcg aaggacggcc caggcgacgt cactgcggcc gacatagagc tcaaggccga
     1861 cgtagaggtc gtagaccccg aggcttacat agcgagcgtc tccgatggcg ggcacctcga
     1921 catgcgcctg acggtcgaga ggggccaggg ctacgcgagg gctgagcaga acaagagcga
     1981 agccgaccct ataggcgtga tcgccgtcga ctcgctgttc tcgccggtac agagggtcaa
     2041 ctacacggtc gcggagacgc gggccggcgc gagggccgac ctcgattcgc tgcgcatgga
     2101 ggtcttcacc gacggacgca tcgagccgca agaggcgttg caggatgcct ccaggcagct
     2161 catggacttt ctcgggttgt tcaccgacgg ctaccagggg accggcgtcc ccgacgcccg
     2221 ccagcgcggc gggcgtcctg tgatcacgga cgagcgcccg gtcgaggacc tcgagctaac
     2281 ggtccgctcc tataactgcc tcaagcgtga gggggtcgac accataggcc agctcgccac
     2341 catgaccgag gaagagctca tgaacatccg taacctcggc atgaagagcg tcgatgagat
     2401 ccgctccaag ctcctggag
//
LOCUS       DASTKM010000342         3849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1213126, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000342 DASTKM010000000
VERSION     DASTKM010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213126"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFQ10_13695"
     CDS             <1..405
                     /locus_tag="VFQ10_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET9966441.1"
                     /translation="TPDLFRCAVDIVGPSSLITLINSIPPYWKPLLATFHERVGNPDT
                     EEDFLESRSPLFFVDRIRIPMLIAQGANDPRVKQAESEQIVAAMKEKGLDYEYLLFED
                     EGHGFARPENRLKFYAAAERFLAKHLGGRAQE"
     gene            complement(431..595)
                     /locus_tag="VFQ10_13700"
     CDS             complement(431..595)
                     /locus_tag="VFQ10_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966442.1"
                     /translation="MEDRGEVCLAEEVERFRRLGLSDGEIASKLGVDVSWVATVVSSG
                     EEETALESAD"
     gene            complement(648..929)
                     /locus_tag="VFQ10_13705"
     CDS             complement(648..929)
                     /locus_tag="VFQ10_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9966443.1"
                     /translation="MRRDRAKYRRRRLGALFVAAALAVALYVGGVAGAEQQTVHYTVA
                     PGDTLWEIAIDHTAVWEDPRPKVEAIREANDLSGYEIYPGMSLKVPSTV"
     gene            1016..1801
                     /gene="dapF"
                     /locus_tag="VFQ10_13710"
     CDS             1016..1801
                     /gene="dapF"
                     /locus_tag="VFQ10_13710"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET9966444.1"
                     /translation="MRFTKMQGCGNDFLIFGPGEVEGMDLHDLATKACDRHFGVGADG
                     ILIPAPSGTADLEMVYLNSDGSTSEMCGNGLRCLARYAKDRDMVEGEAMTVATGAGVK
                     KILLSDDGSSRVDMGAPSFDADVGVGGFRFLRVSMGNPHAVAFLESEDAVETLDLSTV
                     GPPIEEDPVFPERTNVEFGFARGWHDVRMRIWERGAGETLASGSGSCAVAVAAVRLDL
                     AQSPVNVRMDGGMVQIDWSGEDDPVFMNGPAEYVCEGELVLRS"
     gene            complement(1948..2601)
                     /locus_tag="VFQ10_13715"
     CDS             complement(1948..2601)
                     /locus_tag="VFQ10_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9966445.1"
                     /translation="MQRILLVEDHASFRQTLAFVFDQQPEFEVVAQAGTLTEARRTME
                     GLEADLGVIDLTLPDGEGTELIEELCEANPDFAALVLTASLDRTEHARAVEAGAAGVL
                     HKSADVDAILDATRRLGDGETLLSEEELIALLRLAGENREEEVEARASIEQITPREKE
                     VLQKLAEGLSNKEIAASLHMSVDTERTHMMNILNKLGVHSRLQALVFAARHGLVEIR"
     gene            complement(2683..>3849)
                     /locus_tag="VFQ10_13720"
     CDS             complement(2683..>3849)
                     /locus_tag="VFQ10_13720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9966446.1"
                     /translation="GRIGEDAAVSAMKAGADDYLMKDNLARLNSAIERELRDAEVRRE
                     RRQVEEKYRSIFENAVEGIFQTTVDGRFLTANPAMARMLGYESPEDLLGAISNIRDQL
                     YVEPGRRDEFYQLALRDGFVSGFEVQMHGKDGGAVWVSANARAIYDAGGGISGYEGTV
                     ENITERKRAEEALRQIREAERRRIARELHDVVLQDLTYALQSMQVLRRMPEGVDRAGE
                     KENQVEALKRAVGGLRDAIYDLRIEGVRERPLVRAVESIVELNRQISEGCQFELIVDE
                     GFPAVRAGPAGVEVVRVVQEALANVRRHSDARHAVVTLGMVNGEVLVEIEDDGRGFGP
                     ETSYGMGLTGMRERVLVLGGELEVEGREGVGTRVLLRVRLGVLTGGESGERSNS"
BASE COUNT          697 a         1305 c         1113 g          734 t
ORIGIN      
        1 acgcccgacc tcttccggtg cgccgtcgat atcgtgggtc cttcgagcct gatcaccctg
       61 ataaacagca tcccgcctta ctggaagcct ctcctcgcga ccttccacga gcgggtcggc
      121 aatcccgaca ccgaagagga cttcctcgag tcacgctcac ccctgttctt cgtcgaccgc
      181 atcaggatac cgatgctcat cgcccagggc gcgaacgacc cacgcgtaaa acaggccgag
      241 tcggagcaaa tagtcgccgc catgaaggag aaaggcttag actacgagta cctgctcttc
      301 gaggacgaag gtcacggttt cgcccgtccc gagaaccgtc tgaagttcta cgcagccgca
      361 gaacgctttc tggccaaaca cctcggagga cgcgcacaag agtagaggga tacttcgtgt
      421 ggggagcagc tcagtctgct gactccagag cagtctcctc ctcccccgag gagaccacgg
      481 tcgcgaccca gctcacatcg acacccaact tgctcgctat ctccccatca gagaggccga
      541 gccgtcgaaa acgttcgacc tcctcagcca gacagacttc gccgcggtct tccaagctct
      601 ctcctcgcaa caccatcgca gggattcgag gataccacgg acgcagatca gacggtcgag
      661 ggcaccttca ggctcatgcc ggggtagatc tcataccccg agagatcgtt ggcctcgcgg
      721 atggcctcca ccttcggcct cggatcctcc cagaccgcgg tgtgatctat tgctatttcc
      781 cagagcgtat ctccaggcgc aacggtgtag tggacggtct gctgctcagc ccctgccacg
      841 ccacccacgt acagggccac ggcgagggct gcggcaacga acaaggctcc gagcctgcga
      901 cggcgatatt tcgcgcggtc tcttctcacc ggggaaatat acgggacaac tgttccgttc
      961 ccaacggcat tttctcgccg ccaccgcgaa caggccatcc ggtagaatcc gctgtatgcg
     1021 tttcaccaag atgcaaggct gcggcaacga tttcctcatc ttcggtcctg gcgaagtaga
     1081 ggggatggat ctccatgacc tcgccacgaa agcctgcgac aggcacttcg gcgtcggggc
     1141 cgacgggatc ctcatccccg ctccctcggg gacagccgac ctcgagatgg tctacctgaa
     1201 ctctgacggc tcgacctctg agatgtgcgg caacggcctg cgctgcctgg cgcgctacgc
     1261 gaaagaccgc gatatggtgg agggcgaagc catgaccgtc gccaccggcg ctggggtcaa
     1321 gaagatcctc ctctccgacg atggttcctc gcgggtggac atgggggcgc caagcttcga
     1381 cgccgacgtc ggggtaggag gcttccgctt cctgcgcgtc tccatgggca acccgcacgc
     1441 agtagctttc ctggagagcg aggacgcggt cgagacgctg gatctctcta ccgtcggacc
     1501 gcccatcgaa gaagatcccg tcttccccga gaggacgaac gtggagttcg ggttcgcgcg
     1561 gggctggcac gatgtcagga tgaggatctg ggagcgcggc gcgggtgaga ccctagcctc
     1621 gggttcaggc tcgtgcgccg tagcggtcgc agcggtaagg ctggacctcg cccaaagccc
     1681 cgtcaacgtg cgaatggacg gtggcatggt gcagatagac tggtccggcg aggacgaccc
     1741 cgtattcatg aatggccccg ccgagtacgt ctgcgaagga gagctggtac tgcggagctg
     1801 acgagacccg aaagtggccg gccccttccg caagagtctc tcgcacgaat caaaaagccg
     1861 aaatgctgac aagcggaggc cgcaaggccg aagcctgctg aaatgctgaa gtgagcggtt
     1921 ttcaggaagt tccttcccga aaaccgctca gcggatctcg accaggccgt ggcgggcggc
     1981 gaataccagg gcctggagcc gagagtgcac gcccagcttg ttcaatatgt tcatcatgtg
     2041 ggtgcgctcc gtatccacgc tcatgtgcag gctggccgct atctccttgt tcgagaggcc
     2101 ctcggcgagc ttctgcagga cctccttttc gcggggtgtg atctgctcga tgctcgcgcg
     2161 cgcttctacc tcttcttcgc ggttctctcc ggccaggcgt agcagcgcga tcagctcttc
     2221 ctcggagagc agggtctcgc cgtcgccgag acgtcgcgtg gcatcgagta tcgcgtcgac
     2281 atcggctgac ttgtgcagga cgccggccgc cccggcctcg accgccctgg catgctccgt
     2341 cctatccagg ctcgcggtta ggacgagcgc cgcgaagtcc gggttggctt cgcacagttc
     2401 ttcgatcagc tcggttccct ccccgtccgg tagggtgagg tctataaccc cgaggtcggc
     2461 ctccaggccc tccatggtcc ggcgtgcctc ggttagtgtc cctgcctggg cgacaacctc
     2521 gaactcgggc tgctggtcga agacgaacgc gagtgtttga cggaacgaag cgtggtcctc
     2581 tacaagcaag attcgctgca tctctcccct acgcagaccc ctcttgtgat cccccacgca
     2641 cgaaaacaat ataccagctt tggcggttta gacgctgcct gctcaggagt tcgagcgttc
     2701 tccggactcc ccgcccgtca gtacacccag cctcaccctg agaagcacac gggttcccac
     2761 gccttcccta ccctccacct cgagctctcc accgagtacg aggacgcgct cgcgcatccc
     2821 cgtgagcccc atcccgtaag acgtctcggg cccgaacccc ctgccgtcgt cctcgatctc
     2881 caccaggacc tcgccgttca ccataccgag ggtgacaacc gcatgcctgg cgtctgaatg
     2941 gcgcctgacg ttggccagcg cttcctggac aacacgaact acctcgacac cggcaggtcc
     3001 ggctctcacg gccggaaacc cctcatccac gatcagttcg aattggcacc cctcggagat
     3061 ctggcggttc agttcgacga ttgactccac ggcgcggacc aggggccgtt ccctgacgcc
     3121 ctcgatccgc aggtcgtaga tggcgtcgcg caggcctccg accgcccttt tgagcgcctc
     3181 tacctggttt tccttctctc cggcgcggtc aacaccctcg ggcatcctcc tgaggacctg
     3241 cattgactga agggcgtacg tgaggtcctg caagaccacg tcgtgcaact cgcgggctat
     3301 ccgcctgcgt tcggcctccc tgatctggcg cagggcctct tcggcacgct tgcgctcggt
     3361 aatgttctct acagtaccct cgtaacccga gatacctcca ccggcgtcat agatggcgcg
     3421 cgcgttggcc gagacccaca cagcgcctcc gtctttaccg tgcatctgta cctcgaagcc
     3481 gctcacgaag ccgtcccgaa gcgcgagctg gtagaactca tccctgcgtc ccggttctac
     3541 gtaaagctgg tccctgatgt tggagatggc cccaagcaga tcctcgggag attcgtagcc
     3601 caacatcctg gccatcgcgg ggttcgccgt cagaaaccgt ccgtcgaccg ttgtctggaa
     3661 gatcccttcg acggcgtttt cgaagatcga acggtacttc tcctcaacct gccggcgctc
     3721 gcggcgcacc tcagcgtccc tgagctcccg ctcgatggcg ctgttgagcc tggccaggtt
     3781 atccttcatg aggtagtcgt cggcaccggc tttcatggcc gagacggctg cgtcctcccc
     3841 gatccggcc
//
LOCUS       DASTKM010000343         6655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_504976, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000343 DASTKM010000000
VERSION     DASTKM010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504976"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(500..790)
                     /locus_tag="VFQ10_13725"
     CDS             complement(500..790)
                     /locus_tag="VFQ10_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966447.1"
                     /translation="MTTKKPKLPIIPFASPEAREAWLEEHHTTSDGLCLKWWVMNKCI
                     YRRNLNDGSDLGCNAHHHGQGALARFGEVMRRNSWFTSHVAQALVGWATQST"
     gene            complement(983..1513)
                     /locus_tag="VFQ10_13730"
     CDS             complement(983..1513)
                     /locus_tag="VFQ10_13730"
                     /inference="COORDINATES: protein motif:HMM:NF014689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HET9966448.1"
                     /translation="MISLLILGFVLSLDNFRLAIALGAFQLSWRRALRIAVVFGLWDG
                     FSPLIGLLIGRYFGQEIGPAADILGPIVLLVYGLYLVVRSLQTEAPEDLDERMALFGI
                     PLSLSLDNLLAGTGLGMLGFPPVVTAAVFGTITALMTFVGLQLGGVAARFIPIRSDLL
                     SGMGLSIMAVVLVLGY"
     gene            complement(1510..2646)
                     /gene="pqqE"
                     /locus_tag="VFQ10_13735"
     CDS             complement(1510..2646)
                     /gene="pqqE"
                     /locus_tag="VFQ10_13735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HET9966449.1"
                     /translation="MNRPFGMLAELTYACPLHCPYCSNPLNLTDYREELTTAEWQRVL
                     TEARGLGVLQLHLSGGEPLQRRDLVELVASARDLGLYTNLITSALGLSPRRAKQLKTA
                     GLDHVQISIQADEPALSDRIAGTPSYGRKIAAARLVKELAWPLTLNVVLHRHNIDRIG
                     RILDLAEELGADRIELANTQYYGWAWRNRTALLPSRAQLDQAEAVVRAARQRLEGKMQ
                     IIYVIPDYYSQYPKPCMGGWGQKQLTVTPNGGVLPCPAAHTLNLPRATVREHSLAWIW
                     EESPLFQRFRGTEWMPEPCRSCDRREVDFGGCRCQAFQLTGDAARTDPVCHLSPDHRV
                     VAEAVEAANEGAEPIAQGRLVPRAHTKNSNYSPTGGLESPQSVP"
     gene            complement(2639..2920)
                     /gene="pqqD"
                     /locus_tag="VFQ10_13740"
     CDS             complement(2639..2920)
                     /gene="pqqD"
                     /locus_tag="VFQ10_13740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HET9966450.1"
                     /translation="MSIAVLSTNRPHLPPQVRLEWDPVRKRQVLLAPEGVLVLNQTGS
                     SILGLCDGERTVVEIVEELRAQYNRVADDEVRDFLARLVAKRWVELGDE"
     gene            complement(3048..3752)
                     /gene="pqqC"
                     /locus_tag="VFQ10_13745"
     CDS             complement(3048..3752)
                     /gene="pqqC"
                     /locus_tag="VFQ10_13745"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HET9966451.1"
                     /translation="MVSDTEGFIGALRAQSQRYHSWHPFHAMMNEGRLSPRQIQGWVA
                     NRFYYQENIPRKDAAILANCPDREVRRRWIRRIHDHDGTADGEGGIEAWLRLGEAVGL
                     TREEMWDERYVVPGVRFAVDAYITFARTRPWVEAVAASLTELFAPDLMAERLAAFERL
                     YTWIDPAGLAYFRARLTQAPRDSEHALEVVMEHCRTPDEQARAVAALSFKNDVLWSML
                     DAIDSAYSGSTRQEDH"
     gene            complement(3758..4651)
                     /gene="pqqB"
                     /locus_tag="VFQ10_13750"
     CDS             complement(3758..4651)
                     /gene="pqqB"
                     /locus_tag="VFQ10_13750"
                     /inference="COORDINATES: protein motif:HMM:NF003523.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HET9966452.1"
                     /translation="MWGRVLGAAAGGGFPQWNCGCPQCQAVRDGSRPCRSRTQSSVAV
                     SADYRRWFLLNASPDVRAQIESFPALHPHGVRDSPLQAVLLTDAELDHTLGLLLLREA
                     YDLEVHATAAVHETLCRGTSLLQTLDAYTHVEWRPVATGTEVSLADGLSYRAFHVPTN
                     KRARFGTGEGEDGVVGYRITDGETGRALVYLPSAQKLTASVLAHLEGCTCLLFDGTCW
                     QDDELIRLGIAGKTSRDMGHLPIGGADGSLKQLAPLPIERKIYIHINNTNPILLEDAP
                     ERRIVEEHGLEVAADGLELEF"
     gene            complement(4768..4863)
                     /gene="pqqA"
                     /locus_tag="VFQ10_13755"
     CDS             complement(4768..4863)
                     /gene="pqqA"
                     /locus_tag="VFQ10_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HET9966453.1"
                     /translation="MELQKWETPEFEEIGTSAEVTAYAGIWPESE"
     gene            complement(4972..>6655)
                     /locus_tag="VFQ10_13760"
     CDS             complement(4972..>6655)
                     /locus_tag="VFQ10_13760"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HET9966454.1"
                     /translation="QEYVDAGHAASQALMSGVVGSAPPVANDVTYERILDARSEPQNW
                     LTYYGAYDGQRYSPLDQINTENVNRLAPAWVFQAGSVGMHAGPSTYSFEAAPIVVDGV
                     MYLSGWDGWVWALDARSGQELWRYKHAIPYDVSLCCGNVNRGVAVAKGKVFVVTLNAH
                     VLALDAQTGERVWDKTYGDVRAGESATVAPLVVKNLVIVGSSGGEFGTRGHLDAYDID
                     TGERVWRCYMVPKPGEPGSETWPDDGEAWQRGGGNCWVTGTYDPEQNLLYWGTGNPAP
                     DFDGGVREGDNLYTCCVVAVDADNGEIRWHYQYNPHDLWDYDSNMENILFELDGRKLL
                     AHFDKNGYFFVLDRTNGELVQVAPFVDRITWGEIDADGKVTPKVYPENEGETVTFWPG
                     PAGGKEWTHAAYSPRTGLFYAPVQDVGVEVTRRRREFKESIPYWGAAVVVHSEDMAGS
                     VSAFDPSTGEEVWRWRNELPMCASVLATGGDLVFAGQPTGEFNAFDARTGEHLWQFQC
                     GSGHHSSPTTYSVDGRQYVAVPVGWGAWTEGFIPAMLGAPAGDALFVFALPE"
BASE COUNT         1229 a         2153 c         2031 g         1242 t
ORIGIN      
        1 agctggcgca cgcgtgcgcg aggctctggt agaggcgata agacgagctc ttagggccgt
       61 cactacagaa gatactgcag gttggttcgc ccacgccggc tattggccgc aggatcaatc
      121 cttatgagta ctgctgtgag atgatagatg ctgtgatggg ccgagagtat ggggagcgca
      181 aggccgacaa agggcaacgc cgatgcgaag tccgcaccct ctgctcttct cggtgtaggt
      241 cccagagtac aaaaacgaga gaggtgtgct tccgggcact gctaaacgat tctgacgcag
      301 agttgtcact gtcaatagtt tgagacgagc aagcgatgcc cgctacgctt aggacaccac
      361 cgagtcgctg gacggcggga tcgaagacaa tcgagctacg caaggcactg gaacggcgca
      421 cgcctaggcc cgagctaacc catgcccagc cgcaggtgac gggccttcta accactccga
      481 cattcttcct gccaactatc taggtgctct gggtagccca tccgacaaga gcctgcgcca
      541 cgtggctagt gaaccaactg tttcgccgca ttacttctcc gaatcttgct agtgcaccct
      601 gtccatggtg atgcgcgttg cagccaagat cactcccatc gttcaggttc cgccggtaga
      661 tacatttgtt cattacccac cacttgaggc agagcccgtc cgaggtcgtg tggtgttctt
      721 cgagccacgc ctcccgagcc tcaggggagg cgaacgggat tatgggcagt ttgggcttct
      781 tggtagtcat agcgtctcct tttcgactac cggtggcgct gtgcgccgag gagaaccgtg
      841 gaggtctgcc tcttgctcgc cgggcttgag ccgttggtgc cccgcccgaa gaagatctgg
      901 gctgacggcg cctacggtgg caaggaactc gccaagtgaa caggctctga gatcacgctt
      961 tcctcccgat ctcggctggc tcttagtatc ccagtaccag cacgactgcc atgatcgaga
     1021 gtcccatacc actgaggaga tcggagcgga tgggtatgaa gcgggctgcg actcccccga
     1081 gttgcaggcc aacgaatgtc atcagggccg tgatagtgcc aaagaccgct gccgtgacga
     1141 cggggggaaa gccaagcatc cccaagccgg tacccgcgag cagattgtcc aggcttagag
     1201 atagaggaat accgaagagt gccatacgct catccagatc ctctggtgct tcggtctgca
     1261 gactccgaac gacgaggtat agaccataca caagcagcac gatcggtccc aggatgtccg
     1321 ccgctggacc gatctcctgc ccgaagtagc gcccgatcag cagacctatc aggggcgaga
     1381 agccatccca taacccgaac accactgcga tccggagtgc acggcgccag ctgagctgaa
     1441 aagccccgag cgcaatcgcc aaccggaaat tatccaggct gaggacaaag ccgaggatga
     1501 gaagcgatat cacggcacac tctgtggaga ctctaagccg ccggtcggtg agtagttgct
     1561 gttcttggta tgggcacggg ggacaagcct tccttgcgcg atcggctctg cgccctcgtt
     1621 agcagcctct accgcctcgg ccacaacgcg atggtcgggt gagaggtggc agaccggatc
     1681 ggtgcgcgcg gcatcgccgg tgagctgaaa cgcctggcag cggcaaccgc cgaaatcgac
     1741 ctcgcggcga tcacaactcc ggcaaggctc cggcatccac tccgttcccc ggaagcgttg
     1801 gaagaggggc gactcctccc agatccatgc aagcgaatgc tcgcgcacgg tggctcgggg
     1861 aagattgagg gtgtgcgccg cagggcaggg taggacaccc ccgttgggtg tcaccgttag
     1921 ctgcttttgg ccccagccgc ccatacaggg tttggggtac tggctgtagt aatcagggat
     1981 gacgtagata atctgcatct tcccctccag gcgctggcgc gcagcgcgca ctaccgcctc
     2041 ggcctgatcg agctgagccc tgctaggcag tagggctgtg cggttgcgcc aagcccagcc
     2101 gtagtattgg gtattggcca gctcgatccg gtccgctcct aactcctcgg ctagatcgag
     2161 aatgcggccg atcctatcga tgttgtggcg gtggaggacc acattcaatg tgagcggcca
     2221 agccagctcc ttgaccagac gagctgccgc gatcttgcgt ccataggaag gagtgccggc
     2281 tatacggtca gagagggctg gctcgtcggc ctggatgctg atctgtacgt ggtcgaggcc
     2341 ggcggtcttt agctgttttg cccgccgggg cgagaggcct agggcgctcg taatgaggtt
     2401 ggtatacaga cccagatctc gggcgctggc cacaagctct acaaggtcgc ggcgttgcag
     2461 cggctcaccg cctgagaggt gaagctgcag gacgccgagg ccccgcgcct cggtcagcac
     2521 tcgttgccac tctgccgtgg tcagctcctc gcggtaatcc gtgaggttga gcgggttgga
     2581 gcagtacggg cagtggagcg ggcaggcgta ggtgagctcg gccagcatgc cgaacggcct
     2641 attcatcgcc aagctccacc catcgcttag caacgagtcg agcgaggaag tcccgcacct
     2701 cgtcgtcggc cacgcggtta tactgtgcgc gcagttcctc cacgatctcg acgaccgtcc
     2761 gttccccatc gcacaggccc aggatagacg agcctgtctg attcaggacc agtactccct
     2821 ccggtgccag tagcacttgc cttttgcgca ccgggtccca ctccagtcgg acctgtggcg
     2881 gcagatgagg tcgattcgta gatagtaccg caatgctcat gtcacctccc gtgccattgt
     2941 gcgcccgacg tccgccaggt gtggcgcacg cttgataact ggctcaatac aatctgaagt
     3001 tgactggcaa atctgatgcc cggtatacga tagtccactg gatgcaatca atgatcctcc
     3061 tgtcgagtac tgcccgaata ggcactgtcg atagcgtcca acatgctcca cagcacgtca
     3121 ttcttgaagg atagcgccgc cacagctcgc gcctgttcat ccggtgtccg gcagtgctcc
     3181 atcaccacct ccagagcgtg ctccgagtcg cgtggggcct gcgtaaggcg ggcgcggaaa
     3241 taggccagcc cagcggggtc aatccaggtg taaaggcgct cgaacgcggc caggcgctct
     3301 gccatcagat ccggcgcaaa tagctcggtc agcgaggcgg ccaccgcctc gacccacggg
     3361 cgggtgcggg cgaaggtgat gtaggcatcg acggcgaacc gcaccccggg cacgacgtac
     3421 cgctcgtccc acatctcctc ccgcgtcagc cctacagcct cgcccagacg cagccaggcc
     3481 tcgatgcccc cctccccgtc cgctgttcca tcatggtcgt ggatgcgccg gatccagcgt
     3541 cggcgtacct cacggtcagg gcagttggcg aggatagcgg cgtccttacg tgggatgttc
     3601 tcctggtagt agaagcggtt ggccacccag ccctggatct gccgggggct caggcgtccc
     3661 tcgttcatca tcgcgtggaa cggatgccaa ctgtggtagc gctgcgactg agcgcgcagg
     3721 gctccgatga agccctcagt gtcggatacc accaaattca gaactccagt tctagcccat
     3781 ccgcggccac ttccaagccg tgctcctcga cgatgcgccg ctcgggcgcg tcctcgagca
     3841 agatggggtt ggtattgttg atatggatat agatcttgcg ctcgatgggg agcggggcca
     3901 actgcttcag gctaccgtca gcaccgccga tcggtaggtg tcccatatcg cgcgacgtct
     3961 tgccggcgat gccgagccgg atcagctcgt cgtcctgcca gcaggtcccg tcaaagagca
     4021 ggcacgtaca gccttccagg tgagcaagca ccgatgccgt caacttctgt gcgcttggca
     4081 ggtacaccag ggcccggccg gtctctccgt cggtgattcg gtaccctacc acgccatctt
     4141 ccccctcccc ggtcccgaaa cgcgcccgct tgttcgtggg aacgtggaag gcccggtagg
     4201 acaaaccatc cgccagcgag acctctgtcc ctgtagcgac cggccgccac tcgacgtggg
     4261 tgtaggcgtc gagcgtctgg agcagtgagg tgcctctgca gagggtctcg tgaactgccg
     4321 cggtggcatg tacctcgagg tcataagcct ctcgcagcaa cagtagacct agggtgtgat
     4381 ccagctcggc gtcggtgagc agcacggcct gcagcggaga atcccttacc ccgtgggggt
     4441 gcagggccgg gaaagactcg atctgagcac gaacgtcggg tgaagcgttg agcaggaacc
     4501 atcgccggta gtcggcgctc accgcgaccg atgactgggt gcgcgaacgg cagggccgcg
     4561 agccatcgcg cacggcctga cactgagggc aaccgcagtt ccactgcgga aaccctcctc
     4621 ccgctgccgc tccgagtaca cgcccccaca cacccgtcct ccatctacct cgatcattct
     4681 ccttggccgc aatcgcccca cggcaagacg ggcataggcg gacatgaaga aggcgcctga
     4741 ccattcagcc tggcgccgta tattcgccta ttccgattcc ggccagatac cagcgtacgc
     4801 agttacctca gcgctggtcc cgatttcctc gaactcgggg gtctcccact tttggagttc
     4861 catggctatt acctcctttc atatggactt cattgtgcag gctcatcgcc ggagacgttc
     4921 cttccggtac gctgggtggc gtacgccacc cagcgtaccg gacatctgtg cttattccgg
     4981 cagcgcgaag acgaagagag catctccggc aggcgccccg agcatagcgg gtatgaaccc
     5041 ttcggtccaa gcgccccacc cgaccggcac ggctacgtac tgccttccgt cgacgctgta
     5101 ggtcgttggg ctactgtggt ggccgcttcc acactggaac tgccacaaat gctccccggt
     5161 gcgcgcatcg aatgcgttga actcccccgt aggttggccg gcgaacacga ggtcgccgcc
     5221 cgtggccagc acggacgcgc acatcggcag ctcattgcgc cagcgccaca cctcctcgcc
     5281 cgtggacgga tcgaaggcgc tcacggaccc tgccatgtcc tcggagtgca cgacgacggc
     5341 cgctccccag taaggaatgc tctccttgaa ctccctgcgc cgacgcgtga cctccacgcc
     5401 gacatcctgt accggggcgt agaagagccc cgtcctcggg ctgtaggccg cgtgcgtcca
     5461 ttccttgccg ccggccggcc ccggccaaaa ggtgaccgtc tccccctcgt tctcggggta
     5521 caccttgggc gttaccttgc cgtcggcgtc gatctcgccc caggtgatgc ggtcgacgaa
     5581 gggtgctacc tggaccagct caccgttcgt acgatccagg acgaagaagt acccgttctt
     5641 gtcgaagtgc gccagcagct tgcgcccgtc caactcgaac aggatattct ccatattgct
     5701 gtcgtagtcc cacaggtcgt gcgggttgta ctggtagtgc cagcgtatct cgccgttgtc
     5761 cgcgtccacg gcgacaacac agcaggtgta gaggttgtcg ccctcgcgta cgcctccgtc
     5821 gaagtcgggc gccgggttgc cggtccccca gtacaggaga ttctgctccg ggtcgtaggt
     5881 gccggtgacc cagcagtttc caccaccccg ctgccaagcc tcgccgtcat ccggccaggt
     5941 ctcggacccc ggctctccgg gcttcggcac catatagcag cgccagacgc gctctccggt
     6001 gtcgatgtcg taggcgtcga ggtggccgcg cgtcccaaac tccccgccgg agctccccac
     6061 gatgaccaag ttcttcacga ccaggggagc gaccgtagcg ctctcaccgg cccgcacatc
     6121 tccgtacgtt ttgtcccaga cacgctcgcc ggtttgggcg tcgagcgcga gcacgtgggc
     6181 gttcagggtg acgacgaaga ccttcccctt cgctacggcg accccgcggt tcacattgcc
     6241 gcagcagagc gacacatcgt acgggatcgc atgcttgtat cgccacagtt cctgccccga
     6301 ccgggcgtcg agggcccaga cccagccatc ccagcccgaa aggtacatga ctccgtccac
     6361 gacgatcggg gcggcctcga acgagtacgt cgacgggccc gcgtgcatgc cgaccgagcc
     6421 ggcctggaac acccacgcgg gagcgaggcg gttgacgttc tcggtgttga tctggtccag
     6481 cgggctgtag cgctgcccgt cgtaggcgcc gtagtaggtg agccagttct gcggttccga
     6541 gcgggcatcg aggatgcgct cgtacgtgac gtcgttcgcc accggcggcg cgctcccgac
     6601 cacgccgctc attagtgcct ggctggcggc atgccctgca tcaacgtatt cctgc
//
LOCUS       DASTKM010000344         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_861601, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000344 DASTKM010000000
VERSION     DASTKM010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861601"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1311)
                     /locus_tag="VFQ10_13765"
     CDS             complement(25..1311)
                     /locus_tag="VFQ10_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET9966455.1"
                     /translation="MDLFDESGLENLAGRAPLAERLRPATLDDVVGQPHLTDEGGPLR
                     SVVERGRIGSVVLWGPPGTGKTTLARVLANTVEEEFVPLSAVTSGVKDLRAALGGARE
                     RLKYEGRGTLVFVDEVHRFNKNQQDALLPALEEGLVDFIGATTENPSFEVTAPLLSRS
                     RVLRLRSLSQEDLGLLLDRGVEELGVDVSDEAREYLLRLAGGDGRRMLNALEVASTGT
                     KRVEVTDVERAVGQRALRYGREEHYDVISAFIKSVRGGDPDAALHYLARMLEAGEDPV
                     FVARRLVILASEDIGNADPHGLPLAVSAAEAVRLIGMPEGRIPLAQATTYLATAPKSN
                     AAYTGINAALADVRHDNTPEVPMHLRNAVTGLMKEEGYGSGYRYAHDDDPEGMNDTYL
                     PEEISDRVYYEPMESGDEAEIKERLQRWRGERERRK"
     gene            1438..2013
                     /locus_tag="VFQ10_13770"
     CDS             1438..2013
                     /locus_tag="VFQ10_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966456.1"
                     /translation="MEAARERSRGDGGARAAFLLAWSLAGLSVAMFVASVPLLVLARS
                     AQIPSSWEANLTVGDLLGGVIFLIFPLVGALIASRRPRNAIGWILLAEGLLWMFLGMT
                     DYYGLYGVARPGSVPFPVGVAAVNNFMWVPAVGLLGTYVFLLFPDGRLPSRRWRPLAW
                     LSGTVIVLVSILVGLTPGGLQNLGGYATRTR"
     gene            2073..>2361
                     /locus_tag="VFQ10_13775"
     CDS             2073..>2361
                     /locus_tag="VFQ10_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966457.1"
                     /translation="MLASVISLVMRYRRSRGEERQQIKWIAFAASFVGLLYLIAMACA
                     FIFPSGAWFQAGSPLWLDLLGYAALSSFTLVPIAIGFAVLRYRLYEIDIIIN"
BASE COUNT          370 a          757 c          732 g          502 t
ORIGIN      
        1 gcccgatgac gaataaatga gaccctactt ccggcgttcc ctctccccgc gccagcgttg
       61 cagcctttcc ttgatttcgg cctcatcgcc actttccatc ggctcgtagt agacgcggtc
      121 cgagatctcc tcgggtagat acgtgtcgtt cataccttcc ggatcgtcgt cgtgggcgta
      181 gcggtagccc gagccgtagc cctcctcttt catgagccct gttacagcgt tgcggaggtg
      241 catgggaacc tctggcgtgt tgtcgtggcg cacatccgcc agggccgcat ttatgccggt
      301 ataggcggcg ttggatttcg gggccgtggc caggtaggtc gtcgcctgag cgaggggaat
      361 tcgaccctcc ggcatcccga tgagcctgac cgcttctgcg gctgagacgg cgagcggcag
      421 gccgtgcgga tctgcgttgc ctatatcttc tgaggccagg atcacgagcc gcctggcaac
      481 gaacacaggg tcctctcccg cctcgagcat cctggctaga taatggagcg cggcgtcagg
      541 gtccccgcca cgcacgctct tgatgaacgc gcttatgacg tcgtagtgct cctcacgccc
      601 gtacctgagt gccctctgcc ccaccgcacg ctccacgtca gtgacctcga cccgcttcgt
      661 gcccgtggat gcgacttcga gtgcgttgag catccgccgt ccgtcaccgc cggcgagccg
      721 tagcaggtac tcgcgggcct catcggagac gtcgaccccg agctcctcga cgcccctgtc
      781 gagcaggagg ccgagatcct cttgcgagag agaccgcagc ctgagcacgc gcgagcggga
      841 gagtagcggg gcagtcacct cgaaggacgg gttctccgta gtcgccccta taaagtccac
      901 gaggccctct tcgagggcgg ggagcagggc gtcctgctgg ttcttgttga agcggtggac
      961 ctcgtccacg aagacgagcg tccctcgccc ctcgtacttg agccgctcac gggcgccgcc
     1021 cagagctgcc cgaagatcct taaccccact ggtcaccgcg ctcagaggca cgaactcttc
     1081 ttcgaccgtg ttcgccagca cgcgggccag agtcgtcttc cctgtgccgg gcggtcccca
     1141 gaggacgaca gagccgatcc tgccccgctc cacgaccgac cgtagagggc ctccctcatc
     1201 ggtcaggtga ggttggccga ccacatcgtc gagcgtcgcg gggcgcagcc gttcggcgag
     1261 cggggcgcgg cctgccaggt tctccaggcc tgattcgtcg aagaggtcca cctgattagt
     1321 atatcctccg tccggccgaa tccctcgacg acttggtcca ctgtggggtg caaagatgga
     1381 cttcgtaacg ctttccgtaa cgactgggag acggttgtac tgtagagtag cgttgtgatg
     1441 gaagcggcga gggaacgaag ccggggggac ggcggtgcgc gcgcggcttt cttgctggcc
     1501 tggtcgctcg ccggtctctc tgtagccatg ttcgtggcca gcgtcccgct gcttgtactc
     1561 gcccgctcag cgcaaatccc aagcagttgg gaagcaaacc tcaccgtggg tgacctcttg
     1621 ggaggggtga tcttcctgat cttcccgctg gttggggccc tgatcgcctc aaggcgtccc
     1681 cgcaacgcga tcggctggat cctcctggcg gagggtctct tgtggatgtt cttgggtatg
     1741 acggactatt acggcctcta cggtgtagcc agacccggct cggtcccatt tccagtggga
     1801 gtcgccgctg tgaacaattt catgtgggtg cccgccgtgg gactactggg gacctacgtg
     1861 ttcctactct tccccgacgg caggctgccc tccaggaggt ggcgtcctct ggcctggctc
     1921 tccgggacgg taatcgtgct ggttagcatc ttggtcggac tcaccccagg agggttgcag
     1981 aaccttgggg gatacgcaac ccgtacgcgt tagagggcta cccctggctg gaaaccgtag
     2041 cgtacatcgt tctgccactg ttgcccctgt gcatgctcgc ctctgtcatc agcctggtaa
     2101 tgcgttaccg gcgttcgaga ggagaggagc gccagcagat caagtggata gccttcgccg
     2161 cctcgttcgt tggcctcttg tacctgatcg ccatggcttg cgcattcatc ttcccatcgg
     2221 gggcctggtt ccaggcaggc tcaccattgt ggttggacct ccttgggtat gcggcgctga
     2281 gtagcttcac gctggtccct atagccatcg gtttcgccgt tttgcgctac cgcctctatg
     2341 agatagacat catcatcaac c
//
LOCUS       DASTKM010000345         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1038254, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000345 DASTKM010000000
VERSION     DASTKM010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038254"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFQ10_13780"
     CDS             complement(<1..456)
                     /locus_tag="VFQ10_13780"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HET9966458.1"
                     /translation="MHWGSDEELYGTIRRDLFTAVVGDVMDTMGLLRQFLPPQIKPLH
                     DDMVVTGRALTVLEADVFTESPGPGATDSTTESFGLMFKALDDLKAGEVYICAGASPR
                     YALWGELMSTRAIKLGAAGAVLEGYHRDTRGILRLGFPAFSYGGYAQDQA"
     gene            complement(822..1124)
                     /locus_tag="VFQ10_13785"
                     /pseudo
     CDS             complement(822..1124)
                     /locus_tag="VFQ10_13785"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1234..2412)
                     /locus_tag="VFQ10_13790"
     CDS             complement(1234..2412)
                     /locus_tag="VFQ10_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9966459.1"
                     /translation="MSDARSTLYQDLFGLDGLLTEEEREVRERVRAFSDAEVIPIMAD
                     CWERGEFPFQLVPKLAALSICGDTIKGYGCAGLSAVGAGLAAMELARGDGSIYTFFGA
                     TSGFAINTIFNLGSEDQKEKWIPGMARLEKLGAFALTEPEHGSDAVMIETKARREGDE
                     YVIDGKKRWIGNGTVADVIIIWARDENDELGGFLVEKGSPGLQASPITGKASKRPGIQ
                     ADLSLEGVRVPVENRLPGARTFKDTTSALAVVRCGVAWEALGHAIAVYETALAYTRER
                     KQFGRPIASFQLVQDKIAKMLADITTMFLLCIQLGRRAEAGKMTDVAASLAKMNNAQK
                     ARRICLDARDIMGGNGILYEYHVARHWADMEAVYTYEGTDSINSLIVGREITGYSAFS
                     "
     gene            complement(2423..3190)
                     /locus_tag="VFQ10_13795"
     CDS             complement(2423..3190)
                     /locus_tag="VFQ10_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HET9966460.1"
                     /translation="MQVGESTFFITGGGSGLGAATARLLVENGAHVALADVDEEAGEQ
                     MASQIGSGAEFVRTDVTDEESVQDALDSVVEIFGSLNGAVNCAGIGPAARVVGKKGVH
                     DLGLFTKTVEINLVGTFNVIRFAAVRLAENELGEDGERGVIINTASVAAYDGQIGQAA
                     YAASKGGVVALTLPVARELASSGIRVVTIAPGIFETPMLAALPEDAKDSLGKQVPFPP
                     RLGRPEEYAALAKHIVENQMLNGEVIRLDGGIRMAPR"
     gene            complement(3213..>3515)
                     /locus_tag="VFQ10_13800"
     CDS             complement(3213..>3515)
                     /locus_tag="VFQ10_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET9966461.1"
                     /translation="PAAEMALAEAGIPISGVDVIKTHNPFAVNDAYFARQMGVQVKDM
                     NNYGSSLIFGHPQGPTGARLIAEMIEELRLRGGGIGLFTGCAAGDSGAALVVLVED"
BASE COUNT          641 a         1109 c         1047 g          718 t
ORIGIN      
        1 tgcctggtct tgggcgtatc ctccgtagga gaaggcggga aaacccagtc ggaggatccc
       61 gcgcgtgtcg cggtggtagc cctccaagac cgctccggcg gccccgagct ttatggcccg
      121 cgtgctcatc agctcacccc aaagggcata tcggggcgag gcaccagcac agatgtagac
      181 ctctccggcc ttcaagtcgt cgagcgcctt gaacatcaag ccgaaggatt cggtcgtaga
      241 atcagtggcg cctgggccgg gcgactcggt gaagacgtcg gcctccagca ccgtcaaagc
      301 ccgcccggtg accaccatat cgtcgtggag cggtttgatt tgcggtggca gaaattggcg
      361 tagcagcccc atggtgtcca tgacgtcgcc aaccaccgcc gtgaaaagat cgcgacggat
      421 cgtgccgtat agctcttcgt ccgagcccca gtgcatcacg ttctactacc tttccctcgc
      481 ccgtcatggc gctaataaaa gtttaaggtg agcagatggc acgcgccatg aggatcatcg
      541 gtcccccgca ggcgtgcata ttactgtcgc tcctggcaag gctgcccgta tgttcagcga
      601 ggatattcca ccaactcaac gagttgaggt acgaagaaag agccgcagct ttcccgacgg
      661 agcacgtgac aacgttgacc acttatttga ccacttatcc gtgagtgtct acggggttcc
      721 gtgcgggtcg accaacctac ccaagagcgc aaaacggctt tctacggggg gtcatgaagt
      781 tccccggggg tctacgggta cctacgtaag ttgactacga atcagaaggt cgcaggttcg
      841 agtcctgccg agcgcgccac agaaataccg ctatttgcag caaaaaccca cagcacacga
      901 aagcccccga cggagtcatc gggggctttg gcagcagtag gccaagtaag agcttcctca
      961 agggctgtgg cggctacgtc tcccattccc ggcatgacgt gagagtatgt gtccatcgtc
     1021 tgcgaaatcg tggcgtgtcc cagcatttcg gagacaatct tggagttgcc attcttcgac
     1081 agcagattgg tcgcgctcgt gtgccgcagg gagtggaagg tgaacccgga gagtcctggc
     1141 ctttcgagaa gcggctagaa accacgatgg tagatgttgt cggcgttcat tggcttacca
     1201 catcggttgg ggaatgccag gttccgatct tccttatgag aacgcactgt agccggtgat
     1261 ctcgcgcccc actatcaggg agttgataga gtcggtgccc tcataggtgt agacggcctc
     1321 catatcagcc cagtggcggg cgacgtggta ttcgtagagg atgccgttgc cgcccatgat
     1381 gtcgcgggcg tcgaggcaga tccttcgggc cttttgggca ttgttcatct tggcaagcga
     1441 cgcggcaacg tccgtcatct ttcctgcttc ggcgcgcctg ccgagctgta tgcagagaag
     1501 gaacatcgtg gtgatgtcgg caagcatctt cgcgatcttg tcttgcacga gttggaagga
     1561 ggcaatgggc ctgccaaact gcttgcgttc tctggtgtag gcaagcgccg tctcatacac
     1621 cgcgatggcg tgtccgaggg cctcccacgc gacgccgcac ctgacgacag caagggcgct
     1681 ggtagtgtcc ttgaaggtcc tggcaccggg cagcctgttc tctacaggca ccctgacgcc
     1741 ctccagcgag aggtccgcct gaatgcctgg tctcttggag gccttgcccg tgatcgggct
     1801 cgcctggaga ccaggcgagc ccttctcgac gaggaatcct ccgagttcgt cattctcgtc
     1861 gcgggcccag atgatgatca catcggccac cgtgccgttt ccgatccagc gtttcttgcc
     1921 atctataacg tactcgtcgc cctccctacg ggccttcgtc tcgatcatca ctgcatctga
     1981 tccgtgctcc ggttcggtaa gggcgaaggc cccgagcttc tccagccggg ccatgcctgg
     2041 aatccacttc tctttctgat cctcgctgcc gaggttgaag atagtgttta ttgcgaagcc
     2101 ggaagtcgcc ccgaagaacg tgtagatgct cccgtcgccc cgcgccagtt ccatggccgc
     2161 gagccccgct ccgaccgcgc tgagacccgc gcacccgtat ccctttatgg tgtcaccaca
     2221 gatactgagc gcggctagct tcgggaccag ctggaacggg aactcaccgc gctcccagca
     2281 gtcggccatg atcgggatga cctcagcgtc cgagaacgcc cgtacgcgct cgcggacctc
     2341 gcgctcctct tcggttagca gaccgtcgag gccgaagagg tcctggtaca aggtggagcg
     2401 cgcatcgctc acggattcac ctttacctgg gcgccatcct gatgcctccg tcgagacgta
     2461 ttacctcgcc gttgagcatc tggttctcga cgatgtgctt cgctagggcc gcgtactctt
     2521 ccggcctgcc aagcctcgga gggaaaggta cctgcttgcc aagggagtcc ttcgcgtcct
     2581 cgggtagcgc tgcgagcatc ggcgtctcga agatgcctgg ggctatcgtt accacccgta
     2641 taccgctaga ggcgagttcg cgggccacag ggagcgtgag cgcaacgacg ccacccttcg
     2701 aagcggcgta ggcggcctgc ccgatctggc cgtcgtaggc agcgacgctc gcagtgttga
     2761 ttatcacccc tcgctcgccg tcctcgccca gctcgttctc ggccagcctg acggcggcga
     2821 aacggatgac gttgaaggtg cctacgaggt ttatctcgac tgttttcgtg aaaagcccca
     2881 ggtcgtggac gccctttttc ccgacgaccc tggcagccgg gccgatcccg gcgcagttca
     2941 cggcgccgtt caaggaaccg aagatctcca ccaccgaatc aagcgcgtct tgcacgctct
     3001 cttcgtccgt gacgtcggta cgtacaaact cagcgccgct accgatctgc gaggccatct
     3061 gctcaccggc ctcttcgtcg acgtcggcga gggcgacgtg cgccccgttc tccacgagga
     3121 gacgggccgt cgccgccccg agacccgagc ctccacctgt tataaagaaa gtgctctccc
     3181 ctacctgcaa agcttctcct ccctgtggtc cactagtctt ccaccagcac gacgagggcg
     3241 gcgccggagt ctcctgcggc gcagccggtg aagaggccga tcccgccgcc ccgtagcctg
     3301 agctcctcga tcatctccgc gatcaggcgc gctccggtcg gtccctgagg gtggccgaag
     3361 atgaggctgg acccgtagtt gttcatatcc ttgacctgca cccccatctg tctggcgaag
     3421 taggcgtcgt tgacggcgaa cgggttgtgc gtctttatga cgtcaacgcc tgagatgggg
     3481 atgccggctt cggcgagggc catctcggcc gcagg
//
LOCUS       DASTKM010000346         6672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1390759, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000346 DASTKM010000000
VERSION     DASTKM010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390759"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFQ10_13805"
     CDS             complement(<1..323)
                     /locus_tag="VFQ10_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET9966462.1"
                     /translation="MRARVFPDYAAISTAAAYVVAGRLEEEPSSVLMLPTGITPLGMY
                     RRLVEMHRSERLSFANATFFNLDEYLGLAPDHRASYHVYMKEHFYGLIDADPARVFVP
                     DGAAP"
     gene            complement(320..1939)
                     /gene="ptsP"
                     /locus_tag="VFQ10_13810"
     CDS             complement(320..1939)
                     /gene="ptsP"
                     /locus_tag="VFQ10_13810"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET9966463.1"
                     /translation="MKLTGIGASEGVAVGPAFVPATGRPEPERDRITEDEVERELQRF
                     REAVETVAGNLSGTADRLRTSGSEEEAGIFEFHVEMAEDPELASAVEERVGNLESPEA
                     AVLAVGEEFAAEYAAMDDEYMAARADDVRDVTGQISAQLMGRGISAFEALRTPSVVLS
                     QSLAPSDTARIQKGMALGFVTAEGSKTSHVSIMARSMGIPAVVGVGPELERVLGAQTV
                     AVDGGEGYVIADPNADTVAEFERMSGALAEERVALDEYRHVEASTREGRRIEVAANLG
                     SASEADDALKWGAEGVGLFRTEFLFMERDDLPSEDEQYEAYSAVARAFGKKPVIIRTL
                     DVGGDKDLPGVEQGTEENPFLGWRGIRMSLDTPELFEPQLRAILRAAVHGNLKVMFPM
                     VADIGELEAAKAILQECRRELESEGMAVGEVETGVMIETPAAAVCAGELAEQSAFFSI
                     GTNDLVQYTLAADRGNERLRRLQSADHPAVLALIRRTCEAASAAGIQVGVCGEAAGEP
                     EMIPRLVELGVTELSMPAPQIPRAKMVISEL"
     gene            complement(1955..2212)
                     /locus_tag="VFQ10_13815"
     CDS             complement(1955..2212)
                     /locus_tag="VFQ10_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET9966464.1"
                     /translation="MAERETTVGPEEGIHGRPAARFVKAAKGFSSQVVIVKGDREVNA
                     KSVLKITGLARKGDKVTIRAEGEDAEQAVETLAELIAADEH"
     gene            complement(2276..3037)
                     /locus_tag="VFQ10_13820"
     CDS             complement(2276..3037)
                     /locus_tag="VFQ10_13820"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET9966465.1"
                     /translation="MRENRLRKIWQEGGTAVNGWLHVPSSFSAEVMAHAGWDSLTIDM
                     QHGPVDYGRLVPMLQAISTTDTVPVVRVPWNDPGLIMRVLDAGCYAVICPMINTREEA
                     EAFVGACRYPPEGYRSYGPYRATIYGGQDYTDHANETVVTMAMIETQQALENLDEILG
                     VQGLDAVFVGPSDLGQSLGHGPGTDRGEPAVVEAIERVLAAARDHSLAAGIFTGSPEY
                     ASRMAGNGFQFVTISSDARLMASAAASAIAAFESE"
     gene            3363..4127
                     /locus_tag="VFQ10_13825"
     CDS             3363..4127
                     /locus_tag="VFQ10_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9966466.1"
                     /translation="MAERLDGTVAFVTGASSGIGEATAKALARQGAAVAVVARRKDRL
                     ERLAQEIQEDGGKTFAIEADITDQEQAVSAVEHTISEFGRLDTVFNNAGVMLLGPIED
                     APTEEWERMVDLNIKGLLYVAHAALPHLLKAAENSPRRVADLINTSSVAGRRIRSGAG
                     VYNLTKFGVGAFSQALRQEITGRHVRVSLVEPGAVATELQSHLRDEIREQAAARFEDV
                     QILQSEDIADAVVYIVTRPRHVAINEILIRPTEQEG"
     gene            complement(4147..5031)
                     /locus_tag="VFQ10_13830"
     CDS             complement(4147..5031)
                     /locus_tag="VFQ10_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9966467.1"
                     /translation="MAERKDRAAALVEASLLLAVLFLGTNPVAVKVAVAEFPPIPFVA
                     MRFTLAGLLLFVLVALLEPREGRPGRRDLLSLAAVGLVGVGANNVALTLGVSMTTASE
                     TALIYAAVPIWGILLGLALGLERPTRWGILGVCLAFLGVAVVVYGGLTGNTSFLGNLL
                     VVVATVCWGSYAILSLPLLRRYSPLVVASYTMLFGGLGALPLALPGFLDAGWVEASAR
                     AWEALAYSTLLVAAFGFWAWQRGVSQVGANRVLVYQYLITLVGVAAGVILLGESLTAN
                     KVLGGAVILLGVYLARRR"
     gene            complement(5092..>6672)
                     /locus_tag="VFQ10_13835"
     CDS             complement(5092..>6672)
                     /locus_tag="VFQ10_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9966468.1"
                     /translation="EEWREGKLWPKRLHPDDRERILAADERFEAGGEESFSEEYRLLA
                     RDGSVVWVREEAVLLRDEAGEPLYWQGIFYDLTERKALEERLEHRAFHDYLTDLPNRQ
                     LFVDRLRKALDRTRRKRDRKVAVLFTDLDEFKIINDSLGHEAGDVLLTLVAQRLKRCL
                     RPEDSLARFGGDEFVVLIEDIEGPEVAVRVAERITEELKRPFFLEGRELFAPSSIGIG
                     LGDARTKTPESLLMEADTAMYRAKEEGGTFRVFDPDMYARAKGRLELEADLRRTLEAP
                     HKRLPVFYQPMVSIPNGTIVGMEALLRWAHPEQGLLVPPGFIAMAEETGLIVPMGRWV
                     LNEACRRAREWQERYPKDAQLTMAVNISARQLRYPELVGEVEVALRKAALDPGSLTLE
                     ITESVLVEDEGSSAGTLQRLKELGVRLAIDDFGVGYSSLSYLRYLPVDQLKLDRVLVG
                     NLDTDDKNLAIVRAAVDLGHALGIEVVAEGVETREEFVELRKLGCDVGQGDYWWGPRL
                     PRETEELLASNQAPLPDL"
BASE COUNT         1232 a         2260 c         1985 g         1195 t
ORIGIN      
        1 tctggagcgg ccccgtcggg gacgaagacc cgcgcagggt cggcgtcgat gagcccgtag
       61 aaatgctcct tcatgtatac gtggtagctc gccctgtgat ccggcgccag cccaagatac
      121 tcgtcgaggt tgaagaaggt cgcgttcgcg aacgagagac gctctgagcg atgcatctcg
      181 acgagccgcc tgtacatccc gagaggagta atgcctgttg gcagcatgag gaccgaggaa
      241 ggctcttctt ccaacctccc ggcgacgacg tacgccgccg cggtgctaat ggccgcgtag
      301 tccgggaaga cgcgcgccct catagctctg agatgaccat cttggcccgt gggatctgag
      361 gggcgggcat gctcaactct gtgacgccga gctcgacgag tctcggtatc atctcgggct
      421 cgccggccgc ctcgccgcac acgcccacct gtattcccgc cgcgctcgcg gcctcgcagg
      481 tgcgcctgat cagggccaga accgcgggat ggtccgcgct ctggagccgt cgcagccgct
      541 cgttgcccct gtccgcagcc agggtgtatt ggaccagatc gttagtccct atagagaaga
      601 aggctgactg ctcggcgagt tctccggcgc agaccgcggc cgctggagtc tcgatcatca
      661 ctcccgtctc gacctcgcct acggccattc cttccgattc aagctcccta cggcactcct
      721 gcagaatcgc cttcgcggcc tccaactccc cgatgtcagc gaccatcggg aacatgacct
      781 tgaggttacc gtggaccgcg gcgcgcagga tggcacgcag ctggggctcg aagagctcag
      841 gtgtatccag gctcatcctt attccacgcc agccgaggaa agggttctct tcggtgccct
      901 gttccacgcc gggcaggtcc ttgtcacccc ccacgtccag agtacgaatg atgacgggtt
      961 tcttgccgaa agcgcgggcg acggcgctgt aggcttcgta ctgctcatcc tcggagggca
     1021 ggtcgtctcg ctccatgaac aggaactcgg tgcggaacaa tcctacgcct tcggcgcccc
     1081 acttcagggc gtcatcagcc tcagacgccg agccaaggtt cgcggcgacc tcgatgcgcc
     1141 gcccttccct ggtgctcgcc tcgacgtgac ggtactcgtc gagggccacc cgctcctcgg
     1201 ccagcgcccc agacatcctc tcgaactcag cgaccgtatc cgcgttggga tccgcgatca
     1261 cgtagccctc gcccccgtcg accgcgaccg tctgggcgcc caagactcgc tccaactcgg
     1321 gtccgacgcc gacgacggct gggataccca tggagcgggc catgatcgag acgtgcgaag
     1381 tcttcgaccc ctcggcggtg acgaacccga gcgccatccc cttctggatg cgcgctgtgt
     1441 ccgatggtgc gaggctttgg gagagcacga cggagggcgt ccttagtgcc tcgaaggccg
     1501 agatgccacg gcccatcagc tgggcggaga tctggcccgt aacgtcgcgc acgtcatcgg
     1561 cccttgcggc catgtactcg tcatccatcg ccgcgtactc agcggcgaac tcctcaccga
     1621 cggccagcac tgccgcctcc gggctctcca ggttccccac gcgctcctcg accgccgagg
     1681 caagctcggg gtcctcagcc atctcgacgt ggaactcgaa gataccggcc tcctcttcgc
     1741 tccccgaagt acggagcctg tcagccgtcc ccgagaggtt gccagcgaca gtctcgacag
     1801 cctccctgaa cctttgcagc tcgcgttcga cctcatcctc tgttatccgg tcccgctcgg
     1861 gctcgggcct gccggtagcg gggacgaaag ccggccccac ggccacccct tctgacgctc
     1921 ctatgccggt cagcttcacg agcttcgcca ggatctaatg ttcgtccgct gctatgagct
     1981 cggccagggt ctccacggcc tgttcggcgt cctcgccctc cgcgcgaatg gtgaccttat
     2041 cgccctttct ggcaagcccg gttatcttca gaacgctctt ggcgttgact tcccgatcgc
     2101 ccttcacgat cacgacctgc gaggagaatc ccttggccgc ctttacgaac cgcgccgccg
     2161 ggcggccgtg gatcccttcc tcaggcccta ccgtcgtctc tctctctgcc atgaaaaact
     2221 cctctcacgc ccgcgcactc tctacaagac tcggcatacc ggtccaatga taccctcact
     2281 cgctctcgaa agcggctatg gcgctggcgg ctgctgaagc catgagccgt gcgtccgaag
     2341 agatggtgac gaactggaaa ccattccccg ccatgcgcga ggcgtactcg ggagaacctg
     2401 tgaagatgcc ggcggcaaga ctgtgatcac gtgcggctgc cagcaccctt tcgatagcct
     2461 cgactaccgc gggctcgccg cggtccgtgc ctgggccatg cccgaggctc tggccgagat
     2521 ccgagggccc cacgaagact gcgtccaggc cctgtacacc gagtatctca tccaggttct
     2581 ccagcgcttg ctgcgtctcg atcatggcca ttgtcacgac ggtctcgttc gcgtggtcgg
     2641 tgtagtcctg gccgccatag atggttgctc tgtacgggcc gtaactgcgg tatccctcag
     2701 gagggtagcg gcaggccccg acgaaggctt cagcttcttc gcgggtattg atcatcgggc
     2761 agatcacagc gtaacaccct gcgtcgagca ccctcattat gaggccggga tcattccacg
     2821 gcacccgaac gacgggcaca gtgtccgtag tggagatggc ctgcaacatt gggacgagcc
     2881 ttccgtaatc cacgggcccg tgctgcatat ctatggtcag actatcccat cctgcgtgcg
     2941 ccatcacttc ggctgagaag gagctaggta cgtgcaacca cccgtttacg gcggttccgc
     3001 cttcctgcca gatctttctc agacggttct ccctcacctt tgatccctcc cgccgtgcct
     3061 cggttgcccc ggaaacgcgc cgcacgcacc gatccgtcct ggatccaggc cgaggtcccc
     3121 gccggccagt ctcagtagtg ggcgaattcg gtcatttctc tgacatccct gccacgttcc
     3181 acaggatacc agcgtaccag gcggaaggct ccgcgtccca ggagagagta agggaacacg
     3241 cggcggactc cttgcgcccg aaatctcagt gaccggcgcc tgactggtgt ccggttagat
     3301 ccgacgttca tcgggaaaga cattgcctga caaccttttc caatgtgcgg ggaggattgc
     3361 ggatggctga gagactcgac gggacggtgg cgttcgtaac gggcgcgagc agcggtatcg
     3421 gtgaggcgac agcgaaggcg ctcgcgcggc agggtgctgc ggtggccgtc gtcgcccgac
     3481 gcaaggacag gctggagagg ctggctcaag agatacagga ggatggcggc aagacgtttg
     3541 ccatcgaggc ggacataacg gatcaggagc aggcggtatc cgccgtcgag cacacgatct
     3601 ccgagtttgg acggctggat acggtcttca acaacgctgg cgtaatgttg ctcgggccga
     3661 tcgaggacgc gcctaccgag gagtgggaga ggatggtgga cctgaacatc aagggtctgc
     3721 tgtacgtcgc ccacgccgca cttccccacc tcctgaaggc cgccgagaac tctccgagga
     3781 gggttgccga tctcatcaac acgtcctccg tagccggacg ccgtatcagg agcggcgccg
     3841 gagtctacaa cctgaccaag ttcggggtcg gggccttcag tcaggccttg cgccaggaga
     3901 tcacaggcag gcacgtgcgc gtctcgctcg tcgaaccagg cgcggtcgcc acggagcttc
     3961 agagtcactt gcgggatgag atcagggagc aggccgcagc ccgcttcgaa gacgtgcaga
     4021 tactccagtc cgaagacatc gccgacgctg tcgtctacat cgtcacccgc ccccgccacg
     4081 tggcgatcaa cgaaatcctc atccggccga cggagcagga aggataggtt tgatccgcca
     4141 ggatgcctac cgcctccggg cgaggtagac gccaagaagg atgaccgcgc cgcccagaac
     4201 cttgttcgcc gtgagactct ctccgagcag tatcacgccc gccgccacgc caacgagcgt
     4261 gatcaggtac tggtagacga gcacccggtt cgccccgacc tgcgagacac ccctctgcca
     4321 ggcccagaac ccgaaggccg ccaccaagag cgtggagtaa gccagggcct cccaggccct
     4381 tgcgctggcc tcgacccacc cggcgtccag gaagcccggc agggcgagag gcagggctcc
     4441 caggccaccg aacagcatgg tgtaagaggc caccaccagc ggcgagtacc gccgcaagag
     4501 tggcaaggac aggatggcgt aggaccccca gcaaaccgtc gccaccacga ccaggaggtt
     4561 cccgaggaag ctggtgttgc ctgttagacc accgtagacc acgactgcaa cacccaagaa
     4621 ggcgagacag acacccagga taccccagcg tgtcggcctc tccagcccga gcgcaaggcc
     4681 gaggaggatg ccccagatcg gtaccgccgc gtagatcaag gcggtctcgg aagcggtggt
     4741 catgctcact ccaagcgtca aggcgacgtt attcgccccc actccgacga ggcctactgc
     4801 cgccaaggag agaagatccc gccgaccagg ccttccttcc cgcggctcga ggagcgctac
     4861 gagcacgaag agtaggagcc cggccagggt gaacctcatc gccacgaaag gaataggagg
     4921 gaactcggcg acggcgactt tgacggccac ggggttggtg ccgaggaaga gaactgcaag
     4981 gagcagcgag gcttccacaa gagccgccgc cctgtcctta cgctcggcca tgagcccaag
     5041 tctaccgcct ggagcaacat gtccctcccg aggagggaag ggtaatcggg cctataggtc
     5101 gggcaagggg gcctggttgg atgcaagcag ctcttctgtc tccctgggaa ggcgggggcc
     5161 ccaccagtag tcgccctgtc cgacgtcgca tcccaacttc ctaagctcca cgaactcctc
     5221 gcgggtctct acaccctcgg cgaccacctc gatgcccaac gcgtgcccga gatcgaccgc
     5281 cgcccgcacg atagccaggt tcttgtcgtc agtgtccaga tttcccacga gcacccggtc
     5341 taacttcagc tggtccacag gcaagtacct caggtacgac agcgaggagt atcccacccc
     5401 gaagtcgtct atggccagcc gcaccccaag ctccttgagg cgctgaagcg tgcctgcgct
     5461 agatccctca tcctcgacaa ggacgctctc cgtgatctcc agggtaaggc tcccggggtc
     5521 cagggcagcc ttcctcaggg cgacctccac ttcgccaacg agttcggggt aacgcagctg
     5581 acgagccgag atgttcacag ccatggtcag ctgggcgtcc ttgggatagc gttcctgcca
     5641 ctccctggcg cgccggcagg cctcgttgag cacccaccga cccataggga ctatcagccc
     5701 cgtctcctca gccatagcaa tgaatcccgg agggacgagc aaaccttgct ccggatgggc
     5761 ccacctcagt agcgcctcca tccctactat ggtgccgttg ggtatagaga ccattggctg
     5821 gtagaaaacg ggtaatcgct tatgaggagc ctcgagagtt cgtctgaggt cagcttccag
     5881 ctccaggcga cccttggccc gcgcatacat atcagggtcg aacaccctaa aagtcccgcc
     5941 ctcctccttg gccctgtaca tagccgtgtc ggcttccatg agaaggctct caggcgtctt
     6001 cgtcctggcg tcgcccaggc cgatgcctat actggacggg gcgaacagct cacggccctc
     6061 caaaaagaag ggtctcttca gttcctcagt gatcctctct gctacacgca ctgctacctc
     6121 agggccctcg atgtcctcga tgagtaccac gaactcgtcg cctccgaagc gagccaggct
     6181 atcctccgga cgcaggcagc gcttgagccg ctgggcgacg agtgtgagga gcacatcccc
     6241 agcctcgtgg cccagtgagt cgtttattat cttgaattcg tccaggtccg tgaagagaac
     6301 cgccacctta cggtccctct tgcgcctcgt gcgatcgagg gcctttcgga ggcggtccac
     6361 gaagagctgg cggttcggga ggtcggtcag gtagtcatgg aaggcccgat gctcgagccg
     6421 ctcctctagg gccttgcgtt cggtaaggtc gtagaagatc ccttgccagt agagaggttc
     6481 gcccgcttcg tcccttagga ggaccgcctc ctcacgaacc cacaccacag acccgtccct
     6541 cgccagtaga cggtactctt cgctgaagga ctcctcgccc ccagcttcga agcgctcgtc
     6601 ggcggccagt atcctctccc gatcgtcggg gtggagacgc ttgggccaca gcttgccctc
     6661 tctccactct tc
//
LOCUS       DASTKM010000347         5293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_335245, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000347 DASTKM010000000
VERSION     DASTKM010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335245"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFQ10_13840"
     CDS             <1..267
                     /locus_tag="VFQ10_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9966469.1"
                     /translation="TTIEGLWSGTDLTPVQEGFHVEHGLQCGFCTPGMIMTATEFLER
                     NPDPTEAEIREAISGNLCRCTGYENIVKAVQWAARNGSAQEVGA"
     gene            267..2618
                     /locus_tag="VFQ10_13845"
     CDS             267..2618
                     /locus_tag="VFQ10_13845"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HET9966470.1"
                     /translation="MATVQGHTLGDRMPRKEDGRFIRGKGNYVDDVRLPQMLQSTILR
                     SPYAHARINSVDTSAALELSGVHAVITGEDLAEQGLAWMPTLAGDTQAVLATDKVRFQ
                     GQEVAFVVADDTYIAQDALELIDVDYEPLDPVIDSRKALDEDALVIRDDKDGKDDNSI
                     FFWDAGDKEATGKAFEEAEVKVRQEMYYPRIHPAPMETCGAVADYDVSTGEMTVHLTS
                     QAPHAHRTLFAIVSGLPEHQIRIISPDIGGGFGNKVPIYPGYVCAAVASLVLGRPVKW
                     METRSENLMSTSFARDYHMVGELAATSEGKILGARSTILANHGAFDAHAQPGEQYPTG
                     FFPIFTSCYDVPAAYAEATGVYTNRAPGGIAYRCSFRVTEAIYLMERTMDVLADELGM
                     DPVELRRKNFVKEEQMPYISVMGWEYDGGDYEGALQEALRIAGYEELLEEQKRAREEG
                     RIMGIGLSTFTEIVGAGPGKKCHIAGIEMFDAAELRIHPTGKAILKLGVKSQGQGHET
                     TFAQIVAGELGIPAENVEVREGDTDNTPFGLGTYGSRSTPVCGAATAVVTRKVREKAK
                     KIAAHLLEAAEGDVEYEHGRFFVKGSPDQAKTMDEVAFAAYTNVPEGEEAGLEAVTYY
                     DPPEMTYPFGAYMCVVEIDRGTGQVEVKRFIAVDDCGVRINPTVVDGQVHGGLAQGIG
                     TALMEVIDFDEEGNHLNSSFMDYLIPTSMEVPNYELGEFVTPSTHHPLGARGVAESPT
                     VGSPPAVVNAVVDALSPFGVRNVDMPLTPNKIWQLMREHGVEA"
     gene            2624..3595
                     /locus_tag="VFQ10_13850"
     CDS             2624..3595
                     /locus_tag="VFQ10_13850"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET9966471.1"
                     /translation="MLRQDPNSLARRAESLRRAGELAGAERPFALVTVVRTEPATSAR
                     IGDRAIVFADGSLDGWVGGGCIGPTARKEALAALEEGVPRLVRVTPDTAAAQPGVRMA
                     RMTCASEGTADLYVEPFLPRPTLIVAGDSPVAATLAAIAPPLSFRLLTMEATTRLTPG
                     EVPYPQDSWMVVATFGEFDEDATEAGIKLGLPYVGLVASARRAEQVLSGLRQRGLEEG
                     ELAVVRSPAGLPLGTRGQEGISLSVMAEISSLRAESRPSFSGAQERESTGKPHEAVDP
                     VCGMAVEVASARHVVEHGVRRYYFCSSGCKRTFEQEPEKYAARVSLR"
     gene            3592..4188
                     /locus_tag="VFQ10_13855"
     CDS             3592..4188
                     /locus_tag="VFQ10_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9966472.1"
                     /translation="MSVVGVVLGAGRSSRFGPPKQLLPFGDTTLLGQAVRNANSSSLD
                     RTVVVLGRASGELHDAIDFGRAEVVENTAYGTGCASSLLAGLDAAGDDCEALFLLLGD
                     QPGVLPGSIDRALAEWRDERPWAAVCSYRGRLGHPFVFSLEAFGDLKGLHGDKAVWKL
                     IEAYPDRVRQIELDAELPPDVDTPEDYERALIHHQEAS"
     gene            4194..4820
                     /locus_tag="VFQ10_13860"
     CDS             4194..4820
                     /locus_tag="VFQ10_13860"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9966473.1"
                     /translation="MRGPSGVSAIVLAAGGGSRFGGGKLLAKLGGQPIIEAVLDNLRE
                     APVDEIVAVVGADAERLREVCERHGVRTVANEGWERGQSTSVVAGLRASGGRAAVVLL
                     GDQPFVGAEAVERLVAAFAEGAKVAVATYGGKRRNPVLFSRKVWPLLEAELAGDEGAR
                     SFLRRHPELVVEVPCDGVGDPVDVDTREDLWRLEEMRGPLSDYKMAGG"
     gene            4871..>5293
                     /locus_tag="VFQ10_13865"
     CDS             4871..>5293
                     /locus_tag="VFQ10_13865"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9966474.1"
                     /translation="MKINNEFTVGAPIQQAWDTMLNLERIAPCLPGAAIQDEKDEGEY
                     DGTMKVKIGPITANYKGTVKFEEVDEENHRAVLQATGRDARGQGTASATIVSTLQEEA
                     DGTKVSVETDMKLTGRAAQFGRGIAQDVATKMLDQFASC"
BASE COUNT          996 a         1643 c         1810 g          844 t
ORIGIN      
        1 acgaccatcg aggggctgtg gagcgggaca gatctcaccc ccgttcagga gggttttcac
       61 gtcgagcacg ggctgcaatg cggcttctgt acaccgggca tgataatgac cgccacagaa
      121 ttcctggagc gcaatcccga cccgacggag gcggagatca gggaggccat ctcgggcaac
      181 ctatgccgct gcacgggtta cgagaacatt gtcaaggcgg tccagtgggc cgcgagaaac
      241 ggcagtgcac aggaggttgg tgcgtaatgg ccacggtgca gggacatacc ctcggcgacc
      301 ggatgcctcg caaggaggac gggcgcttta tcaggggcaa gggaaactac gtcgatgacg
      361 tcagactgcc gcagatgctg cagtcaacca tcctgcgcag cccttacgcc cacgcgagga
      421 tcaatagtgt agacacgagc gccgcgctgg agctctccgg cgtccacgcg gtcatcacgg
      481 gcgaggacct cgccgagcag ggcctcgcct ggatgcccac cctcgccggg gacacccagg
      541 ccgtgctcgc caccgacaag gtgcgcttcc aggggcagga ggttgccttc gtggtggccg
      601 atgataccta catcgcgcag gacgccctcg agctcatcga cgttgactac gagccactcg
      661 accctgttat agattctagg aaggcgctcg atgaggatgc tctcgtcatc cgcgacgata
      721 aggatggcaa ggacgacaac tccatcttct tctgggacgc aggggacaag gaggccacag
      781 gcaaagcgtt cgaggaggcc gaggtaaagg tccgccagga gatgtactac ccgcgcatcc
      841 accctgcccc gatggagacc tgcggggccg tcgccgatta cgacgtctcg accggcgaga
      901 tgaccgtcca cttgacctcg caggccccac acgcccacag gacgctcttc gccatcgtct
      961 ccgggctgcc ggaacatcag atccggataa tctcccccga catcggcggc ggtttcggga
     1021 acaaggtccc catctaccct ggctacgtgt gcgccgccgt cgcctccctc gtgctcggcc
     1081 gacccgtcaa gtggatggag acgcgctcgg agaacctgat gagcacgagc ttcgcgcgcg
     1141 actaccacat ggtaggagag ctcgcggcca ccagcgaagg aaagatcctc ggcgcccgct
     1201 cgaccatcct cgcgaaccac ggggctttcg atgcccacgc ccagccgggg gagcagtatc
     1261 ctacgggctt cttcccgatc tttacgagct gctacgacgt gccagccgcc tacgccgagg
     1321 cgaccggtgt atacaccaac agggccccgg gcggcatcgc ctaccgctgc tcgttcaggg
     1381 tgacggaggc catctacctc atggagcgca cgatggacgt gctcgccgac gagctcggga
     1441 tggaccctgt ggagctcagg cgcaagaact tcgtcaaaga ggagcagatg ccctacatct
     1501 cagtcatggg ctgggagtac gacggcggcg actacgaggg cgcattgcag gaggctttgc
     1561 ggatcgccgg ctacgaggag ctcctcgaag agcagaagcg tgcccgtgaa gagggcagga
     1621 tcatgggcat aggtctctcc acgttcacgg agatcgtggg ggcaggccct ggcaagaagt
     1681 gccacatagc gggtatcgag atgttcgacg ccgcggagct cagaattcac cctacgggca
     1741 aggcaatcct caagctcggg gtcaagtcgc aggggcaggg ccacgaaacg accttcgccc
     1801 agatcgtggc aggagagctc ggcatcccgg cggagaacgt cgaggtgcga gagggcgaca
     1861 cggacaatac gcccttcggg ctcggtactt acggtagccg ctccacgcct gtgtgcggcg
     1921 cggctacggc ggtggtcacc cgcaaggtgc gcgagaaggc gaagaagata gcggcccatc
     1981 tcctcgaagc cgccgagggg gacgtcgagt acgagcacgg gcgcttcttc gtcaagggtt
     2041 cgccggatca ggcgaagacc atggacgagg tagccttcgc cgcttacacg aacgttcccg
     2101 aaggagaaga ggccggcctc gaggccgtca cctactacga cccgccggag atgacctacc
     2161 cgttcggcgc ctacatgtgc gtggtggaga ttgacagggg tacggggcag gtcgaggtga
     2221 agcgctttat cgcggtggac gactgcgggg tcaggatcaa ccctacggta gttgacggcc
     2281 aggtgcacgg cggtctcgcg cagggtatcg gcacggccct gatggaggtc atcgacttcg
     2341 acgaagaggg taaccacctg aacagcagct ttatggacta cctgatcccc acgtctatgg
     2401 aggtgcccaa ctacgagctc ggcgagttcg taaccccgtc gacgcaccac ccgctcgggg
     2461 cccgcggcgt tgccgagtcc ccgacggtgg gttcgccccc agccgtggtc aacgccgtgg
     2521 tcgatgcgct ctcgcccttc ggggtgcgca acgtagatat gcccttgacg ccgaacaaga
     2581 tctggcagct catgcgcgag cacggggtgg aggcctgagg ccgatgctcc gccaggaccc
     2641 gaactccctc gcccgtcgcg ccgagtcact gaggcgggct ggagagcttg cgggcgcgga
     2701 acgtcccttc gccctggtca cggtcgtccg caccgagccg gccacctcgg cgcgcatagg
     2761 cgaccgcgcg atcgtcttcg cggacgggag cctcgacggg tgggtcggcg gcgggtgcat
     2821 cgggccgaca gcccgcaagg aagccctcgc cgccctcgag gaaggcgtgc cgcggctcgt
     2881 acgcgtcacc cccgacacag cggccgccca gcccggggtt cgcatggccc gcatgacgtg
     2941 cgccagcgaa ggcacagctg acctctacgt ggagcctttc ctcccccgtc cgaccctcat
     3001 cgtcgccgga gactccccgg tcgcagccac gctcgcagcg atagcgccgc ctctgagctt
     3061 caggctgctc acgatggagg caaccacgag gttgaccccg ggagaagtcc catatccaca
     3121 ggactcctgg atggtcgtcg ccacgttcgg cgagttcgac gaggacgcca ccgaggcggg
     3181 tataaagctg ggcttgccgt acgtcgggct cgtcgcgagc gcccggcggg ccgagcaggt
     3241 actctcaggg ctgcggcagc gcgggctcga ggagggagag ctcgccgtgg tgcgctcccc
     3301 tgccgggctg ccgctgggta cgagaggtca ggaaggcatc tccctctccg tcatggcgga
     3361 gatctcctcg ctgcgcgcgg agtcgcgccc cagcttctca ggagcacagg aacgagaatc
     3421 gactggaaaa ccccacgaag ctgtggaccc ggtctgcggg atggcggtcg aggtggcttc
     3481 tgccaggcac gtcgtcgagc acggagttag gcgttactac ttctgctcct ccggatgcaa
     3541 gcggaccttc gagcaggagc cggaaaagta cgcggcccgg gtctccttac gatgagcgtg
     3601 gtcggcgtgg tgctcggcgc agggagatcc agccgtttcg gccctccgaa gcagctcctg
     3661 cctttcgggg ataccaccct tctcggccag gccgtcagga acgccaattc ctcatcactt
     3721 gacagaacgg tcgtcgtgct tggccgtgcg tcaggcgagt tgcacgatgc catcgacttc
     3781 ggccgtgctg aggttgtgga gaatacagcc tacggcacgg gatgcgcctc ctcgttgctg
     3841 gcgggtctag acgccgccgg agacgactgc gaagccctct ttctgctcct cggcgaccag
     3901 cctggcgtgc tccccgggtc catagaccgg gcgctcgcgg aatggagaga cgaacggcct
     3961 tgggcggcgg tctgctcgta caggggtcga ctcggccatc ctttcgtctt ctcgctggaa
     4021 gctttcgggg atctcaaagg tctgcacggg gacaaggcgg tctggaagct catcgaggcg
     4081 tacccggaca gggttcgaca gatcgaactc gatgctgagt tgcctcctga cgtggatacc
     4141 ccggaggact acgagcgggc gctcatccac caccaggaag cctcctagtc gccgtgcggg
     4201 gaccgtctgg agtatcggcg atcgtgcttg ccgcgggcgg cgggagccgg ttcgggggag
     4261 ggaagttgct agccaaactc gggggacagc cgatcataga agcggttcta gacaacctgc
     4321 gcgaagcgcc cgtcgacgag atagtcgccg tcgtcggcgc ggacgcggag agactgcgcg
     4381 aggtctgcga aaggcacggc gtcaggaccg tcgctaacga agggtgggag cggggccagt
     4441 ccacgtcggt cgttgccgga cttcgggcgt ctggtgggcg ggccgcggtg gtcttgctcg
     4501 gggaccagcc gttcgtcggg gctgaggccg tggagcggct cgttgctgct ttcgcggagg
     4561 gagcaaaggt tgcggtcgcc acctacggtg ggaagcggcg caatcccgtc ctgttctcgc
     4621 gaaaggtgtg gccgctgctc gaggcggagt tggcaggaga tgagggggcg cgttctttcc
     4681 tgcggcggca tcctgagctg gtggtcgagg tgccgtgcga cggagtgggg gaccccgtgg
     4741 atgtcgacac gagggaggat ctgtggcgtc tggaggagat gcgcgggcct ctgagcgact
     4801 ataagatggc gggaggatag taggtagtaa ccgaaagacg taagatagct gttccttagg
     4861 aggaggtacg atgaagatca acaacgagtt cacggtggga gccccgatcc agcaggcctg
     4921 ggacaccatg ctcaacctgg agaggatcgc gccctgcctg ccaggggcgg ccatccagga
     4981 tgagaaggac gaaggcgaat acgacggcac gatgaaggtg aagatcggcc cgatcacggc
     5041 caactacaaa ggcaccgtaa aattcgagga agtcgacgag gagaaccacc gcgccgtcct
     5101 ccaggccaca ggccgcgacg cacgcggcca gggcacggcc tcggcgacca tcgtatcgac
     5161 cttgcaggaa gaagccgacg gcaccaaggt cagcgtcgag acggacatga agctcaccgg
     5221 acgcgcggcc caattcgggc gaggcatcgc ccaggacgtg gctacgaaga tgctcgacca
     5281 gttcgcctcc tgc
//
LOCUS       DASTKM010000348         5550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_507952, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000348 DASTKM010000000
VERSION     DASTKM010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507952"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VFQ10_13870"
     CDS             complement(<1..1340)
                     /locus_tag="VFQ10_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HET9966475.1"
                     /translation="MNEGKTGNRGSDVPPEMAPFDVWSEWMRANMGPMMATPGASVPW
                     LMMPGVRTGEEFAELPEGAVRNDPLLSTIEKLWDANPMQNVLPIDWVEITRALQTLWA
                     REMSDPARAMERATDFNARLFGATMRSWQEATNRLWGLQTSQEEDEGRPDKRFSAPDW
                     EQNPFYETLKESYLLASEYLLDEAEKTDGEDTPEQRRLKFHLKQFVDAMAPVNYLLTN
                     PAALKRAFETGGASLAEGARNLLADLQEGRMSMTDANAFQPGENLAITPGKVVYRNEL
                     IELIQYEPTTDQVHETPVMFIPPWINKYYILDLQPKNSFVKYLVDEGYTVFMISWKNP
                     DASMEDTEFDDYMTKGPLAAAEVVRDITGSEKVNPVGYCVGGTLLAITLSWIAAGEDE
                     NPFGPPTFMVALHDFTEVGDTEVFIDEPQIEFMEQQMLERGYLDNRKMANMFNLL"
     gene            complement(1452..2354)
                     /locus_tag="VFQ10_13875"
     CDS             complement(1452..2354)
                     /locus_tag="VFQ10_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9966476.1"
                     /translation="MTITESTSIDYDINGDGPPLLLISGLGFGRWGWFKQVPAFSRHF
                     RTITFDVRGERTLENGVHDLVSDVVSLLDHLGVKKAHVLGASLGGFVGQELALLRPDL
                     VDRLVLVCTSYGGRGPESMSLRALSDMLGWGSLSPEGVVRRGLETATSGTYRAEHGDE
                     FEQIVSWRLADSPSGATYYDQARAGAGFDLSRGVEHITSPTLVIHGAEDRYVPVANAA
                     ALAGSIPGATLRVLDHAGHLVFIERFADVNREVVSFLGPRGSRKQVGARLQRARRRAR
                     VLGWIRKVPRSLGRWSGRLWDSIF"
     gene            2641..3840
                     /locus_tag="VFQ10_13880"
     CDS             2641..3840
                     /locus_tag="VFQ10_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9966477.1"
                     /translation="MAVTSERTHREKRPVDLIDVGSLLSDEEKLVRERVREFVDREVI
                     PVAADYWDRAQFPFELLSGLGELGLMGGVLPEEYGCAGWNNVAYGLAVAELARGSGSL
                     ATFLHVQSGLAMTAIHELGSEEQKRRWLPPMARCEKIGCFGLTEPGAGSDPGSLSTTA
                     VQKDGGYVLSGEKKWIGNASFSHVAVIWARTDEGKISGFLVETENPGFRADVLPRKAS
                     QRAAWQTHITLEDCHVPEDARLPKAEGLGSTLSILTHSRYGVGWDGLGQATDCYEVAL
                     AYAKEREQFGLPIASFQLVQQKLVEMVNEISLSQLLSIHVGRLKDAGGLDAATVSMFK
                     MNNVAKARQIAALARDVLGGNGILLDYRVMEHMADIEGVYTYEGTNDVNTLIVGQAIT
                     GHRAFSP"
     gene            complement(3993..5204)
                     /locus_tag="VFQ10_13885"
     CDS             complement(3993..5204)
                     /locus_tag="VFQ10_13885"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET9966478.1"
                     /translation="MSFGNGNGIGEIIISTPLRTGIGTFGGSLKDTPATELGATVGKE
                     VLSRAGIEGDQVDQVIVGNILSAGQGMNPGRQVGIKSGLPVEVPGMTLNRMCGSGLQA
                     IVSAAQEIALGDADVVMAGGIENMDRAPFLLDKGRYGYRMGMPKADIYDHMVYDGLWD
                     IFNDYHMGVTAENVAEEYGITREDSDAYAVRSHQRAAKAHEEELFDGQIVPVEVKQKK
                     ETVQFTTDEHIRANASVEGLQKLKPVFKKDGGTVTAANASGINDGAAMMLVSNERKAE
                     ELGMPVAGRLVSAGVAGVDPSVMGTGMIPASRLALKKAGLSVDDLDAVEANEAFASIA
                     IAVERTLEVPEEKMNPVGGAVALGHPIGATGAVLTVKILHHLARTGGQYGLVTLCIGG
                     GMGIAAIFERA"
     gene            complement(5310..>5550)
                     /locus_tag="VFQ10_13890"
     CDS             complement(5310..>5550)
                     /locus_tag="VFQ10_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966479.1"
                     /translation="QELNAQLTQDFFNGVINNLRTQAEGNRALSEDLIEQQAKQREAS
                     RALAKESVNAYTDFLNSMFAYYKGNLDEIQRRANQ"
BASE COUNT         1002 a         1774 c         1698 g         1076 t
ORIGIN      
        1 agcaaattga acatgttggc catcttgcgg ttgtcgagat agccgcgctc gagcatctgc
       61 tgctccatga actctatctg cggctcgtct atgaagactt ctgtgtcccc cacctccgtg
      121 aagtcgtgca acgcgaccat gaaggtcggc gggccgaagg ggttctcgtc ctcgccagcg
      181 gcgatccagg agagcgtgat cgcgagcagc gtccccccta cacagtagcc cacggggttc
      241 accttctcag aacccgtgat gtccctaacg acctcggccg ctgcgagcgg tcctttggtc
      301 atgtagtcat cgaattcggt gtcctccatc gaggcgtccg ggttcttcca ggagatcatg
      361 aacaccgtgt agccctcgtc caccagatac ttgacgaagc tgttcttggg ctgcagatcc
      421 aggatgtagt acttgttgat ccagggcggt atgaacatga cgggggtctc gtgtacctga
      481 tcggtcgtcg gttcgtattg gatcaactct atgagctcgt tgcggtagac gaccttgcct
      541 ggggtaatag ccaggttctc tccgggctgg aaggcgttgg cgtcggtcat gctcatgcgg
      601 ccttcttgca ggtccgcgag caggttgcgg gcgccctcgg cgaggctggc tcctccggtc
      661 tcgaaggccc gcttgagggc agcggggttg gtcaacagat agttgacagg agccatggcg
      721 tctacgaact gcttgaggtg gaacttcaga cgccgctgct cgggtgtgtc ctcaccatcg
      781 gtcttctccg cctcatcgag cagatactcc gaggcgagca ggtatgactc cttgagggtc
      841 tcgtagaatg ggttctgctc ccagtcgggc gccgagaacc gcttgtcggg acggccctcg
      901 tcttcctcct gcgatgtctg gaggccccag agacggtttg tggcctcctg ccagctcctc
      961 atcgtggcgc cgaacagcct ggcgttgaag tccgtagccc gctccatggc tcgcgcgggg
     1021 tcggacatct cccgcgccca gagggtctgc agcgcgcgtg tgatctcgac ccagtcaatg
     1081 ggcaaaacgt tctgcatagg gttggcgtcc cacagcttct cgatggtaga gagcaagggg
     1141 tcgttccgca cggcaccctc gggcagctcc gcgaactcct ctcctgtcct gacacctggc
     1201 atcatgagcc acggtacgct cgcgcccggc gtcgccatca tgggccccat gttcgcccgc
     1261 atccactcag accacacgtc gaacggcgcc atctcgggcg gaacatcgga accgcggttg
     1321 ccggtcttgc cttcgttcat ctagtcttcc tcctctgaga gattaacccg gcggccagcg
     1381 ccgccatcta gtattcgacc gtactattct acccagtgtg tctgtcgccc aaaaacggcc
     1441 tgcaagggag ctcagaagat actgtcccac agcctccccg accagcgccc gagcgaccgc
     1501 ggcaccttcc tgatccaacc cagtaccctg gcccgccgcc tcgcccgctg taagcgggcg
     1561 ccaacctgct tgcgggaccc gcggggcccc aggaaggaga ccacctctct gttcacgtcg
     1621 gcgaaccgct cgatgaagac gaggtggccc gcgtggtcga ggacccgtag cgtggcgccc
     1681 gggatggacc cggcgagggc cgcagcgttg gcgacgggca cgtagcggtc ctcggcgccg
     1741 tggatcacaa gggtgggtga ggttatgtgc tcaacaccac gggagaggtc gaagccggcc
     1801 cctgccctgg cctggtcgta gtaggtagcg ccggagggcg agtcagcgag acgccagctt
     1861 acgatctgct cgaactcatc accgtgctcg gcgcgatagg tacccgaggt cgccgtctcc
     1921 agaccacgtc tgacgactcc ctccggagaa agggaccccc atccaagcat gtccgaaagg
     1981 gcccgcaaag acatcgactc cggacccctg ccaccgtagc tggtgcagac cagaaccaga
     2041 cggtcgacca ggtctggtct gagcaacgcc agctcctgcc ccacaaagcc gccgagcgaa
     2101 gctcccaaaa catgggcctt ctttacaccc aggtggtcca gcagcgacac cacgtcactg
     2161 accaggtcat ggacgccgtt ctcgagcgtg cgctcacccc tgacatcgaa ggtgatggtc
     2221 cggaagtggc gcgagaatgc gggcacctgc ttgaaccaac cccagcgacc gaagccaagg
     2281 ccgcttatga ggagcagggg cggtccatcc ccgttgatgt cgtagtcgat gctcgtgctc
     2341 tcagtgatcg tcaccggcat tcgctactca cctcgattct agcgtcacag agagagcccg
     2401 ccagctggaa acggaaccgg tcgccccaga tcccgtatca aaccaactca tccctccatg
     2461 aggttaaatg cactttgcac ttaagtgcga cacgaatgta ttctagaggg tctggcatta
     2521 ggagcaagtt gatggcgtga aagccatcac tcctggattg accggatcgc cgccagaacc
     2581 tatgctcttc ccgagagggg tgctgggtag tgcccggaat cggagcatgg aggaaaccga
     2641 atggcggtta catcggagag aacgcaccgg gagaagcggc ctgttgacct catagacgtg
     2701 ggtagtctgc tctcggatga ggagaaactg gtcagggaac gggtccgcga attcgttgac
     2761 cgcgaggtca tcccggttgc ggcagactat tgggacaggg cgcaattccc gttcgagctc
     2821 ctctcaggtc tcggggagct cggcctcatg ggtggcgtcc tccctgagga gtatggttgc
     2881 gccggatgga acaacgtggc ctacggcctt gcggtcgccg agctggcgcg ggggtcgggg
     2941 agcctggcta cctttttgca cgtccagagc gggctcgcga tgacggccat ccacgagctg
     3001 ggatcggagg agcagaagcg ccgttggctg ccgcctatgg ccaggtgcga gaagatcggc
     3061 tgtttcgggc tgacagaacc aggtgcagga agcgaccctg gctcgctgag tacgacggcc
     3121 gtccagaagg acggcggcta tgtactcagc ggcgagaaga agtggatcgg caacgcatcc
     3181 ttctctcacg tggccgtgat ctgggccaga acagacgaag gtaagatctc cggtttcctc
     3241 gtcgagaccg agaatccagg gttccgggcc gacgtactcc cccgcaaggc gtcccagcgc
     3301 gccgcctggc agacgcatat cacgctagag gactgccacg tgcccgagga tgccaggctg
     3361 ccgaaagcag aaggccttgg ctccaccctc tctatcctca cgcactcccg ctacggcgtg
     3421 ggatgggacg ggcttgggca ggcgacggac tgctatgagg ttgctctggc gtatgcgaaa
     3481 gagcgggagc agttcgggct gcccatagcc tccttccagc tcgtacagca gaagctcgtc
     3541 gagatggtca atgagatctc actcagccag ctactctcca tccacgtcgg acgcctcaag
     3601 gacgcgggcg gcctcgacgc ggcgaccgtc tcgatgttca agatgaacaa cgtcgccaag
     3661 gcgcgccaaa tcgccgcctt ggcgcgagac gttctcgggg gaaacggcat cctgctggac
     3721 taccgagtga tggagcacat ggccgatatc gagggcgttt acacctacga gggcaccaac
     3781 gacgtcaaca ccctcatagt gggccaggcc ataacgggcc accgcgcctt ctccccttga
     3841 cccccgcaac gcgccgaaga gtaacgacga accaggccga tctcgctccc agtgcatcca
     3901 cctgatggga ttcttcccga acggggcgtt tcgacaagcg aaagggccaa ggcgtagagc
     3961 ctcggccctc gctgactagc taaagttgac tgctacgcgc gctcgaagat cgcggcgatg
     4021 cccatgccgc cgccgatgca gagcgtcacc aggccgtact ggccgcccgt gcgggcgagg
     4081 tggtgcagta tcttgacggt caggacggcg cctgtggccc cgatggggtg tccgagggcc
     4141 actgccccgc ctaccgggtt catcttctcc tcgggcacct cgagcgtacg ctccacggcg
     4201 atagcgatgg aggcgaaggc ctcgttcgcc tcgacggcgt cgaggtcgtc gaccgagagc
     4261 cctgccttct tcagggccag cctggaggct gggatcatac ccgtacccat aacggaaggg
     4321 tcgaccccgg ccacgcccgc tgagaccagc ctgcccgcca cgggcatccc gagttcctcg
     4381 gccttgcgct cgttgctcac gagcatcatg gcagccccgt cgttgatgcc cgaagcgttg
     4441 gcggcggtta ccgtgccgcc atccttcttg aagaccggct tcagcttctg aaggccctcc
     4501 acgctcgcgt tcgcgcggat gtgctcgtcc gtggtgaact ggacggtctc cttcttctgc
     4561 ttgacctcga cgggtacgat ctggccgtcg aagagttcct cttcgtgagc cttggcggcc
     4621 cgctgatggc tgcgcacggc gtaggcgtct gagtcctcgc gcgttatgcc gtactcctcg
     4681 gcgacgttct cggccgtcac gcccatgtgg tagtcgttga agatgtccca caggccgtcg
     4741 tagaccatgt ggtcgtagat atcggctttg ggcatcccca tcctgtagcc gtagcggcct
     4801 ttgtccagca ggaaaggcgc cctgtccatg ttctcgatac cgccggccat gaccacgtcg
     4861 gcgtcgccca gggctatctc ctgcgccgca gacactatgg cctgaagccc cgagccacac
     4921 atcctgttca gggtcatgcc tgggacctcc acggggaggc cgctctttat gccgacctga
     4981 cgaccggggt tcatgccctg gcccgcggag aggatgttac cgactataac ctggtcgacc
     5041 tggtcgccct cgatcccggc gcgagagagg acctctttgc cgaccgtggc cccgagttcc
     5101 gtggccggcg tgtccttgag cgaaccgccg aaagtcccta tacccgtcct gagaggcgtc
     5161 gagatgatta tctcgccgat accgtttccg ttgccgaagc tcaccttttc tccttccgtc
     5221 tactctcgat tagaggatac atcaaaagac aaggcccaga ccacaaggcc cgggcctctc
     5281 tatacgctct tcagcgcgtc gccctccggt tactgattgg ccctgcgctg tatctcgtcc
     5341 aggttgcctt tgtagtaggc gaacatggag ttgaggaagt ccgtgtaggc gttcaccgac
     5401 tccttggcca gagccctgga ggcctcccgc tgctttgcct gctgctcgat caggtcctca
     5461 gacaaagccc tgttgccctc ggcctgggtc ctcaggttgt tgataacgcc gttgaagaag
     5521 tcctgggtca actgggcgtt gagttcctgt
//
LOCUS       DASTKM010000349         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_336633, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000349 DASTKM010000000
VERSION     DASTKM010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336633"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            474..1319
                     /locus_tag="VFQ10_13895"
     CDS             474..1319
                     /locus_tag="VFQ10_13895"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET9966480.1"
                     /translation="MTELQTLMTGIAFGESPRWHDDRLWFSDWGTQEIIAVDLEGESE
                     VIVRLPSFPFCIDFLPDGRLLIVSASDRLLLRREPHGPLVTHADLSTLSEHPWNDIVV
                     DGQGNAYIGNIGFDFPEAEFAPGTLALVTPHGSARQVADGVAFPNGIVVTPDNSTLIV
                     AESYANRLTAFDITADGSLSNRRMWAELGGGVPDGICLDAEGAVWYGDVPNKRCVRVR
                     EGGEVLQTIDLDRGCFACMLGGPDGKTLFMVAREWSGMESTAEEERTGQLLTSPAPAA
                     GAGWP"
     gene            1549..2184
                     /locus_tag="VFQ10_13900"
     CDS             1549..2184
                     /locus_tag="VFQ10_13900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9966481.1"
                     /translation="MDHEEVGRFWNANADSWTKLARAGYDVYRDYLNTPAFFDMLPNV
                     AGLAGLDIGCGEGHNTRLLAKCGAQVTAIDIAENFIANARQLEAHEPLDIAYRVASAV
                     DLPFAEGTFDFATAFMSFMDIPETDRVLAEARRVLKPCGFLQFSISHPCFDTPHRRNL
                     RDAHGLTYAIEVGDYFRNLDGEVDEWLFARGEQGAASCLSFRSRAAAELRR"
     gene            2282..2761
                     /locus_tag="VFQ10_13905"
     CDS             2282..2761
                     /locus_tag="VFQ10_13905"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9966482.1"
                     /translation="MLLRRGRPEDAEGLFQIRNEPEVVRWWGSDDIEEISDQFIEADE
                     GFVIEAGGEVVGAIQYHEEDDPMYRHAGIDIFLTASRHGQGLGTEAIRLLARHLFEER
                     DHHRLTIDPAADNAAAIRAYEKVGFRRVGIMRHYERGPDGAWHDGLLMDMLREEFRP"
     gene            2775..3308
                     /locus_tag="VFQ10_13910"
     CDS             2775..3308
                     /locus_tag="VFQ10_13910"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HET9966483.1"
                     /translation="MVSADPIVVVPYDDAWPSLYEEERARIERAIGPWAEGIEHVGST
                     AVPGLAAKPVIDIMVGVKSLDDSSILVERLVGIGYEYVPEFERVLPFRRYFRKMREGR
                     RTHQIHLVERSNTEWWDRHLLFRDYLRADPEIAGEYAHLKYELSDRFGEDRVAYTDAK
                     THFISEVVRRAQENERS"
     gene            3504..>3759
                     /locus_tag="VFQ10_13915"
     CDS             3504..>3759
                     /locus_tag="VFQ10_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966484.1"
                     /translation="MKTNEHQELAASITAPMRATGGRVFLEIIPSTLDLLARSKPASP
                     EEIAAISAKSPEEVRAALDRFPSAEWDEQGRVVGLGLTLQL"
BASE COUNT          739 a         1123 c         1181 g          716 t
ORIGIN      
        1 tgagcgtccg gaccgtcgaa cgccacgtcg ccaacatcta cgcgaagatc ggcgcccggg
       61 gcagggccaa cgctacggcc tacgcgctca cccataacct gatctagctc ttcattccca
      121 agccctacgc gtttaccgac acggaagggc ccttcggagc ttacttcttg cctgagaaat
      181 gggtgtttac cccgatggcg ggcgccgacc atgacgatag gctcttagca agatatccaa
      241 gatcctgatg aaggttatgc gatgggcaac gcacgctagc gggcatccat gccggtgact
      301 ggctcgtgaa gctactcctc acggctgtca taatcggcgt gtggcgtatc gatggagggt
      361 tgaaagtaag caggtgacgt atctctgaat ccgagagatc gtcgcggaca tctccagcgt
      421 cagtaccgta ccgtctagta ccaccctggt tcccgagaga taggagaagg tgagtgactg
      481 aactgcaaac cctgatgacc ggcatcgcgt tcggcgagtc gccacgctgg cacgatgacc
      541 gcctgtggtt ctccgactgg ggcacccaag agatcatcgc cgtcgatctc gagggtgaga
      601 gcgaagtcat cgtccgcttg ccgtcattcc cgttctgcat cgactttctg cccgatgggc
      661 gactgcttat cgtctcggcg agcgatcggc tcctgctgcg ccgggagccc cacgggccgc
      721 tggtgaccca tgccgacctg agcactctct ctgagcaccc ctggaacgac atagtcgtgg
      781 acggtcaggg taacgcctac atcggcaaca taggcttcga cttcccagag gccgagttcg
      841 cccccggaac cctggcgctg gtcacccccc acggctcggc tcggcaggta gcggatggcg
      901 tcgccttccc caatggcatc gtcgtgaccc ccgacaactc gacgctgata gtggctgaat
      961 cctacgccaa caggctcaca gcgttcgaca tcacggccga cggaagcctg tccaaccggc
     1021 gtatgtgggc cgagctcggc ggcggcgtcc ccgacggcat atgcctcgat gcggaaggtg
     1081 ccgtctggta cggggacgtt ccgaacaaac ggtgtgtgcg cgttcgcgaa ggtggcgagg
     1141 tgctgcagac tattgacctt gaccgcggtt gcttcgcttg catgctcggg ggtccagatg
     1201 gcaagacgtt gttcatggtg gcgcgggagt ggagtggcat ggagagcacg gctgaggagg
     1261 aacgaacggg ccagttgctg acgtcccctg cgcctgccgc gggcgccggc tggccgtagg
     1321 agaggagcag cgtgtacctt ggggcttcct gccctagcgc gacgtgccac cttacctggc
     1381 ccaggctgcc cgtgttgttt gcgactgcga gtcgatgctc agtatgactt cgggttggag
     1441 cacatcctca ccaaagagct gctataaact cgcatctctg aagcctggtc tgtagacaca
     1501 ccatcgtcca gcgcgagcgt ttgccgcagc aagtgagagt gaattgagat ggatcacgag
     1561 gaagtaggac ggttctggaa tgccaacgcc gactcctgga cgaaactggc gcgagccgga
     1621 tatgacgtct accgagacta cctcaacacc ccagccttct tcgacatgct gccgaatgta
     1681 gcagggcttg cagggttgga tatcgggtgt ggagaaggcc acaacaccag gctattggcg
     1741 aagtgtggag cccaggtaac ggccattgac atagcggaga acttcattgc gaacgccaga
     1801 caattggagg cacacgagcc attggacatc gcttatcgcg tcgccagcgc tgtggacctc
     1861 ccttttgccg aaggtacctt tgacttcgcc acggctttca tgagctttat ggacattccg
     1921 gaaacggatc gggtcctcgc tgaagcccgt cgcgtcctga agccctgtgg tttcttacag
     1981 ttttcaatct cccatccgtg cttcgatacc ccgcaccgcc gaaatctgcg tgacgcacac
     2041 ggtctgacct atgcgataga agtcggcgac tactttcgga atctggatgg tgaggttgac
     2101 gagtggctct tcgcgcgtgg cgaacaaggc gctgccagct gtctgtcctt ccgctccagg
     2161 gcagcggctg agcttcgtcg ttaggcggtc gaggcgttcg agaacggcat gagcatgttc
     2221 aacccataga gcgtgacgaa aggggtattg gatctatatg gtcattctgc agggagggcg
     2281 cgtgctgctg cgtcgcggtc gaccggaaga cgccgagggg ctgtttcaga tccgaaacga
     2341 acccgaagtc gtccggtggt ggggcagcga tgacatcgaa gagatcagcg atcagttcat
     2401 agaggcagac gaaggcttcg tcatcgaggc cggcggggag gtcgtcggcg caatacagta
     2461 ccacgaggaa gacgacccga tgtatcgcca tgcgggcata gacatctttc tcaccgcgtc
     2521 ccgccacggc caggggttgg gcaccgaagc gatccggctg ctcgcccgcc acctcttcga
     2581 ggagcgtgac caccatcgcc tcacgatcga tccggcggcc gacaacgcgg ctgcgatccg
     2641 tgcttacgag aaggtgggct tccgcagggt cgggatcatg cgccactacg agcgtggccc
     2701 cgacggagcc tggcacgacg ggctgctgat ggatatgctt agggaagagt tccgcccctg
     2761 accgactctg ccgcatggtg agcgctgacc ccattgtcgt ggtcccttac gatgacgctt
     2821 ggccctcgct ctacgaggaa gagcgggcgc gtatcgaacg tgctatcggt ccttgggcgg
     2881 aggggatcga gcacgtgggc agcacggcgg tgccggggct tgcggcgaag ccggtcatag
     2941 acatcatggt cggcgtgaaa agcctcgacg actcgtcgat cctcgtcgaa cgcctcgtgg
     3001 gcatcggcta cgagtacgtt ccagagttcg agcgggtcct gcccttcagg cgctacttcc
     3061 gcaagatgcg agaggggcgg cgcacccacc agatccacct cgtggagaga tctaacaccg
     3121 agtggtggga tcgccaccta ctctttcgcg actacctgcg tgcggaccct gaaattgcag
     3181 gagaatacgc tcatctgaag tacgagctct cggaccgctt cggcgaagat cgggtggcgt
     3241 acacggatgc caagacccac ttcatctcgg aggtcgtccg tcgcgcgcag gagaacgagc
     3301 gaagctaact gccgaggcgg gcaccctccc gcgccactgt accactccgg cttcgaagtc
     3361 ctactcctac gcgtttaccg acattagtgg tgctgcggag cctaatttct tgcccgaaga
     3421 tgggtggtta ccccgatggc acgcaccggc cctcgcgata ggctggtccg taaagtatcc
     3481 aagtgcccga gaggagacac cagatgaaaa ccaatgagca ccaggaactg gcggccagta
     3541 tcacggcgcc gatgagggcc acgggtggga gagtcttcct cgagatcatt ccatcgacgc
     3601 tcgatttgtt ggccaggagc aaaccggcgt ccccggagga gatcgccgcc atctccgcca
     3661 agtccccgga ggaggtgcga gccgcactcg accggtttcc aagcgccgag tgggacgagc
     3721 aaggcagggt cgtcggcctg ggcttgaccc tccagctga
//
LOCUS       DASTKM010000350         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_157769, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000350 DASTKM010000000
VERSION     DASTKM010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157769"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..467)
                     /locus_tag="VFQ10_13920"
     CDS             complement(33..467)
                     /locus_tag="VFQ10_13920"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET9966485.1"
                     /translation="MSREDPSPVGGGGCLCGAVRYEVRGPLRPVVDCHCTMCRRTSGH
                     FAAFTATRPEDLVLIESEGLRWYRSSMSARRGFCGTCGSSLFWEPASGDRVSIAAGTL
                     DSPTGLETVVHVFVEDAGDYYEIGDGLPQRADGDHGIMIPAD"
     gene            complement(562..1908)
                     /locus_tag="VFQ10_13925"
     CDS             complement(562..1908)
                     /locus_tag="VFQ10_13925"
                     /inference="COORDINATES: protein motif:HMM:NF005812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9966486.1"
                     /translation="MIEKTQRWVEKDRRYVWHALSGYDPDASTMVVAEADGAWITDTD
                     GNRYLDGMSGLWCVNVGYGREELARAAYEQLKEMPYYPLLKSHLPAIRLGEKLNEWLE
                     DDYVFLFSNSGSEANEVAFKVARQYHEQNGEPGRYKFISRYRAFHGQTMGALAATGQA
                     QRKYKYEPLVPGFLHVQPPDRYRCHYCSDRESCTLECAREIERAMSWELPETVAGVIM
                     EPIITGGGIIVAHDDYMRTVEDICERNGALLIVDEVICGFGRTGRRFGYQHYGVKPDI
                     VTMAKGLTSAYFPLAATAVRQEVYETFAGNEEYGRLRHINTFGGHPGGCAVALRNLKI
                     MEEEDLPGRSEEMGRRLREDLTELESHPNVGEIRSKGLLFGIELVEDRESKQTASKEM
                     VEAAVSGCKERGLVISKNADTVAGFNNVLTLAPPLSITEDDLEFIARALKETLTNL"
     gene            complement(1970..3424)
                     /locus_tag="VFQ10_13930"
     CDS             complement(1970..3424)
                     /locus_tag="VFQ10_13930"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET9966487.1"
                     /translation="METAKNFVGGSLVDARVREEIDVPDPATGELLGRAPVSGPEDVD
                     RAVRAALKAYEEWSLEPVTQRARRMFRLQVLLEERIDELAEIATRENGKHVEESTAEI
                     RRGIEVVELAASMTTLMKGETLDQVSAGVDVALYREPLGVVTAITPFNFPVMIPLWFA
                     PLAVAAGNCFILKPSPRTPLSANRLAELFAEAGFPDGVFNVVHGARGTVEALIDHPLV
                     RAVSSVGSAATAKNIYTRAAERGKRVQALGGAKNHMVVMPDANLDVAAKGVFSSAFSN
                     GGQRCLAGSVGVAIGGVGEELVERLTAIARESQVGPGLDPEMDSATGVTPVTTAEAKE
                     RIEGWISRGEEEGASLVFDGRREYDAGEGFFLGPTIFDYVEPQMDLAREEVFGPVLAV
                     ERMEDLDDAIRTINESRYGNAAAIFTSDGKAARRFRREVQCGMIGVNISVPAPVAYFP
                     FTGWKDSFFGDLHATGRDGVEFYTERKVVIERWQ"
     gene            complement(3799..>4187)
                     /locus_tag="VFQ10_13935"
     CDS             complement(3799..>4187)
                     /locus_tag="VFQ10_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966488.1"
                     /translation="DFFRDAQAMQAQLAERTKELLGIPESLPEFGAAPGAAPGGVPPG
                     AEVEPDVSTGDLPPRAGPEVSPESDVLAGTAPDAAFPPGGEGAPREEPPPGEERPEKR
                     GEAPTTPPSPREEPPAGEERPGRRTP"
BASE COUNT          665 a         1481 c         1237 g          804 t
ORIGIN      
        1 cgacggcatc tttgcggtcg cagcgtggag gatcaatcgg cggggatcat gatgccatga
       61 tcgccatccg ccctctgagg caaaccgtcg ccgatctcgt agtagtcgcc tgcatcctcg
      121 acgaacacgt gaaccaccgt ctcgagcccc gtcggtgaat ccaacgtgcc agcagcgatc
      181 gaaacccgat cgccggaagc aggctcccag aaaaggctcg atccgcaggt tccacagaac
      241 cctcgtcgag ccgacataga cgatcggtac cagcgtaacc cttccgactc gatcaggacc
      301 aagtcttccg ggcgcgtcgc cgtgaacgcc gcgaaatggc cgctggtacg gcggcacatc
      361 gtgcagtggc aatctaccac gggacgtagc ggtccacgca cttcgtaccg gacggcgccg
      421 caaaggcagc caccgccccc gaccggcgat ggatcttctc gtgacatact acctccgcaa
      481 ctccggtact cgccgacgga gcgttcctcc acttcccaac tttcgcggga tcctcgaggc
      541 agacctcgtc atttagccta cctacaggtt cgtgagcgtc tccttcaacg cccttgcgat
      601 gaactccagg tcgtcctcgg tgatgctaag gggcggggcg agggtcagca cgttgttgaa
      661 gccggcgacc gtgtcggcgt tcttgctgat gacgaggccg cgctccttgc atccggagac
      721 cgccgcctcg accatctcct tactcgccgt ttgcttgctt tccctgtcct cgaccagctc
      781 gatgccgaaa aggagcccct tgctccggat ttctcccacg ttggggtggc tctccagctc
      841 cgtgaggtcc tcgcgcaggc gcctacccat ctcctcggag cgtccgggta ggtcttcctc
      901 ctccatgatc tttaggtttc tcagcgccac ggcacagccg ccggggtggc cgccgaaggt
      961 gtttatgtgc ctgaggcgcc cgtactcctc gttccccgcg aacgtctcgt agacctcctg
     1021 cctgaccgcc gtggcggcga gcgggaagta ggcgctggtt aggcctttcg ccatcgtgac
     1081 aatgtcgggc tttacgccgt agtgctgata gccgaagcgc ctgcccgtcc taccgaagcc
     1141 gcagatgacc tcgtccacga tcaggagggc gccgttcctt tcgcaaatgt cctccacggt
     1201 ccgcatgtag tcgtcgtggg caacgatgat ccccccgccg gtgatgatgg gctccatgat
     1261 gacgcccgct accgtctctg gcagctccca ggacatcgcg cgctctatct cccgggcaca
     1321 ctctagggtg caggactccc tatccgagca gtagtgacag cggtagcggt ccggcggctg
     1381 gacgtgcagg aagccgggga ccagaggctc gtacttgtac ttcctctggg cctggcccgt
     1441 ggccgccaga gcccccatgg tctgcccgtg gaaggcccgg taacgggaga tgaacttgta
     1501 ccgccccggc tcgccgttct gctcgtggta ctggcgagcg accttgaacg ccacctcgtt
     1561 tgcttcggag ccggagttgg agaagaggaa gacgtagtcg tcctcgagcc actcgttcag
     1621 cttctcaccc agccggatcg cgggcaggtg gctcttgagc agcgggtagt agggcatctc
     1681 cttgagctgc tcgtaggccg cccgcgccag ttcttcgcgg ccgtatccga cgttgacgca
     1741 ccacaggccg ctcatgccgt ccaggtaacg gttgccgtcg gtgtcggtga tccaggcccc
     1801 gtcggcctcc gcgaccacca tcgtggaagc gtcggggtcg tagcccgaga gagcgtgcca
     1861 gacgtacctg cggtccttct cgacccaccg ttgggttttt tcaatcacgc taatattctc
     1921 ctcccattgt tcgcgctgac gacttgctcc accgatcggt tctcgcgatt cactgccacc
     1981 gctcgatgac caccttgcgt tcggtgtaga actcgacgcc gtcgcgaccc gtggcgtgca
     2041 ggtccccgaa gaaggagtcc ttccaacccg tgaacgggaa gtaggccacc ggggcgggca
     2101 cgcttatgtt cacccctatc attccgcact gcacctcgcg ccgaaacctc ctggccgcct
     2161 tgccgtcgct cgtgaagatc gccgccgcgt tgccgtagcg ggactcgttg atggttctta
     2221 tggcatcatc gaggtcttcc atgcgctcaa cggcgagaac agggccgaat acttcctccc
     2281 tggccagatc catctgcggc tctacgtagt cgaagatggt gggcccgagg aagaaaccct
     2341 cgccggcatc gtactcccgc cggccgtcga agacgaggct agcgccctcc tcctcaccgc
     2401 gggagatcca gccttctata cgctccttgg cctcggcggt cgtcacgggg gtgacgcccg
     2461 tggcggagtc catctccggg tcgaggcccg gacccacctg ggactcccgc gctatggccg
     2521 tgagcctctc cacgagctcc tcgcccacgc cgcctatcgc cacccccacg ctgcccgcca
     2581 ggcaccgctg cccaccgttc gagaacgccg aggagaacac acctttggcc gcgacgtcga
     2641 ggttcgcgtc aggcatcacg accatgtggt ttttggcacc gcccagggcc tgcaccctct
     2701 tgccccgctc ggctgcccgt gtgtagatgt tcttggccgt ggcagcgctg cccacgctcg
     2761 agacggccct caccagcggg tggtcgatga gggcctcgac cgtccctctg gccccgtgca
     2821 ccacgttgaa caccccgtcc gggaagccgg cctcggcgaa gagctcggca agacggttgg
     2881 ccgatagcgg ggtgcgcgga gacggcttga ggataaagca attaccggcc gccaccgcta
     2941 ggggcgcgaa ccacagcggg atcatgaccg gaaagttgaa cggcgttatg gccgttacga
     3001 ctcccaaagg ctccctgtac agggcgacgt caacccccgc actaacctga tctagggtct
     3061 cgcccttcat cagcgtggtc atcgaggccg cgagctcgac gacctctatg ccccgcctta
     3121 tctccgccgt ggactcctct acatgtttgc cgttctcgcg cgtggcgatc tccgcgagct
     3181 cgtcgatacg ctcctctagt agcacctgta ggcgaaacat ccgcctcgct ctctgggtta
     3241 cgggctccag gctccactcc tcgtaagcct tcagggccgc ccgcacggcc cggtccacgt
     3301 cctccggccc cgaaacgggc gccctgccca gcagttctcc ggtcgccgga tccgggacgt
     3361 cgatctcttc cctcacccgt gcgtcgacta gactgccccc gacaaagttc ttcgcggtct
     3421 ccatggcaac agctcctccg agcgatggtc gttttcccgc ttcccctttg gccgcgtgtg
     3481 cagccgtgct tggccccata tgtccccaac cttaccaaac agtatgccgg acaagacaga
     3541 atgcgctcgc gctcggcgcc tgcgtaggtc ctcggaggac caccgccatg gcgaggctcg
     3601 aaggcggcaa gaagccgctc ccgtccgaag cgtgagacca gatgagagac tgcctcccta
     3661 gagcggccag tcagtagagg ccaaaatcct cgccaccttg cccataacgg cgttattagc
     3721 cattatcgtg ggccaatgag cgccaaaatc ccttagcggg tcttttcgtt ccggcaatga
     3781 tacgcggagg cccctaggct acggcgtacg cctccctggc ctctcctcgc ccgccggtgg
     3841 ttcttcgcgc gggctcggcg gtgtcgtggg cgcctctccc cgcttctcgg gcctctcctc
     3901 ccccggcggc ggctcctccc tcggcgctcc ctccccgccg ggcgggaaag cggcgtcggg
     3961 cgcggtgccc gctagcacgt cgctttcggg cgatacctcg ggccccgctc gtggcgggag
     4021 atctcccgtc gagacgtccg gttcgacctc ggctccgggc gggacacctc ctggcgccgc
     4081 gcccggtgcg gcgccgaact ccggtaggga ttctgggatg ccgagtagct ccttcgtccg
     4141 ctccgctagc tgcgcctgca ttgcctgcgc atcccggaag aagtcgg
//
LOCUS       DASTKM010000351         4999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_689688, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000351 DASTKM010000000
VERSION     DASTKM010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689688"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..489)
                     /locus_tag="VFQ10_13940"
     CDS             complement(58..489)
                     /locus_tag="VFQ10_13940"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9966489.1"
                     /translation="MDYARKPLPRTEGGYDLVVSALSVHHLTHGDKRELFEKVRHSLA
                     GGGHFVNADQVSGETSEEEALYREWWQRRVREAGISEEDLAAALTRMRADRNATLVAQ
                     LAWLGEAGFEGIGCRYKDHRFAVYNGRKGHEDSENGKDADG"
     gene            complement(586..792)
                     /locus_tag="VFQ10_13945"
     CDS             complement(586..792)
                     /locus_tag="VFQ10_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966490.1"
                     /translation="MTVGTMFGHAAWDYDRARRQLVPGFDRFYGAVLESIPFGEQQEI
                     RVLDLGAGRVSSARWWQGSLRGRG"
     gene            complement(789..1571)
                     /locus_tag="VFQ10_13950"
     CDS             complement(789..1571)
                     /locus_tag="VFQ10_13950"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9966491.1"
                     /translation="MILPREWDAGEYEKLSAPQTRWGVEVLERMEVRGDEAAIDAGCG
                     TGRVTEPLLAKLPEGSVLAIDGSLSMVEAARRRFAGEPRVRVERQDLLSLEVEERVDL
                     IFSTATFHWIWDHDRLFENLARAIKPGGRLVAQCGGEGNISRATRATKETMEEERFLD
                     YFVGWEDDKYYADARTTARRLEAAGFEEIETWLHGEVAAFDSVDELARFLGTVVLGGH
                     LEKLPEEERGPFAAGVAEKVAAVDGKPALDYVRLNMMARRSA"
     gene            1616..2332
                     /locus_tag="VFQ10_13955"
     CDS             1616..2332
                     /locus_tag="VFQ10_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET9966492.1"
                     /translation="MDSIEVVSSRSKSGVGVLDKAVAILDFLSGDGPASLSEVVEGTR
                     LPRPTAHRLLSALETHHLVSRRDGRYVLGLRLLGWGNRAAGVGLVERARPVLEGLRDE
                     SGESTQLYVREGEQRVCVAAVERVTGLRDTVPVGAVMPLSRGSAGKVLLAWAPGGVDG
                     RPDAAQLLGIRVRGWAESVAEREAGVASVSAPVFGEDGRLRAAVSVSGPISRLGESPG
                     QRLSHLVLGAAREIERATGS"
     gene            2638..3480
                     /locus_tag="VFQ10_13960"
     CDS             2638..3480
                     /locus_tag="VFQ10_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9966493.1"
                     /translation="MPEESRVAGPARRPGMAGTVRRLLRLILVVVLLVGASQGTTLAA
                     PASWDYVALGDSLATGFGAFTGYVPRYEAHIETDTGVAVTRTNLGRNGWTSSQLLSAL
                     RSDPTFRRATREAEIVTWNIGGNDLRAARNSYKKGTCGGRDNQDCLRASVATLKTNWT
                     AITAEVLELRSTENTMVRTMDIYNPYVRTDIASDTWKNDGGMNDFQVFNSYVYKANLH
                     IATTSYAAGVSYAGVHLAFNGPYGYEDPKSKGYLSFDGLHPNNTGHRVIAGELQKLGY
                     APLR"
     gene            3739..4083
                     /locus_tag="VFQ10_13965"
     CDS             3739..4083
                     /locus_tag="VFQ10_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9966494.1"
                     /translation="MTEGVKTIMYPVTDLANAKTLYGELLGVEPYRDEAYYVGFNIEG
                     QNIGLDPHGHSEGMTGPVAYRHVNDIEERLQALLDAGAAAHQGGRDVGGGKLLSSVKD
                     TDGNVIGLIQAL"
     gene            4223..4630
                     /gene="tsaA"
                     /locus_tag="VFQ10_13970"
     CDS             4223..4630
                     /gene="tsaA"
                     /locus_tag="VFQ10_13970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HET9966495.1"
                     /translation="MKTPYEIRPIGHVVSPLVDRESAPKQGSEGAPDAWLVFDPDVAE
                     GIRDLAVGTDIFVLTWLHQARRDVLAVHPRDDPRNPEKGVFSTRSEDRPNPIGLHRVH
                     IAAIDGPRVLVRDLEAFDETPIVDVKPVLADGR"
     gene            complement(4732..4914)
                     /locus_tag="VFQ10_13975"
     CDS             complement(4732..4914)
                     /locus_tag="VFQ10_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966496.1"
                     /translation="MSELEGLVEGLRWAFYAGVGLTGSVRVGIAEELSGTELDRDVGA
                     FSRRSQAHGAGGHGRT"
BASE COUNT          962 a         1624 c         1493 g          920 t
ORIGIN      
        1 ccctcggcgc tccgtaccac atgcttctcc cacaccttct ctaccaacgt cttcggccta
       61 tccatcagcg tccttcccat tctccgagtc ctcgtgtcct ttcctaccat tgtagaccgc
      121 gaacctgtga tctttgtatc tacaaccgat cccctcgaaa ccggcctccc cgagccaggc
      181 cagctgcgcc acaagtgtcg cgttcctgtc ggccctcatg cgcgtgagcg cggcggccag
      241 atcctcttcc gagatccctg cctcgcgcac ccgccgctgc caccattccc tgtagagcgc
      301 ctcctcctct gacgtctcgc cggagacctg atcggcgttg acgaaatgtc cgcctccggc
      361 gagtgaatgg cggaccttct cgaacagctc cctcttgtcg ccatgagtga gatggtggac
      421 cgagagcgcc gacacaacga ggtcgtaacc tccttctgtt cggggcaaag gcttgcgcgc
      481 gtaatccatg gtccgaaact cgagtccgaa actcgaaacg gccctgttcc cccgcgaagc
      541 gtcggcgcgc caccctgagc atctccaccg agaggtccac gagcgtcacc cgcgcccgcg
      601 taaacttccc tgccaccacc gcgctgagga gacccgcccg gccccaaggt cgagcacccg
      661 gatctcctgt tgttccccga aagggatgct ctccagcacg gccccgtaaa agcggtcgaa
      721 gccgggcacg agctgccgcc tggccctatc gtagtcccac gcagcgtgcc cgaacattgt
      781 cccgaccgtc acgccgacct cctcgccatc atgttcaagc gaacgtaatc cagggcaggc
      841 ttgccgtcca ccgcagccac cttctctgcc acccccgctg cgaaggggcc ccgctcctcc
      901 tcaggcagct tctccaggtg cccgcccagc acgaccgtcc cgaggaagcg ggcgagctcg
      961 tccaccgaat cgaacgccgc gacctcgccg tgcagccagg tctcgatctc ctcgaagccc
     1021 gcggcctcca gacgcctcgc cgtcgtcctg gcgtcggcgt agtacttgtc gtcctcccag
     1081 cccacgaaat agtccaaaaa gcgctcctct tccatcgtct ccttcgtcgc cctcgtggcg
     1141 cgggagatgt taccctcgcc cccgcactgg gcgacgaggc gtccacccgg ctttatggcc
     1201 cgcgccagat tctcgaagag gcggtcatgg tcccatatcc agtggaacgt tgctgtggag
     1261 aagatcagat ccacacgctc ctcgacctcc agcgacaaca gatcctggcg ctccacccgc
     1321 acccgcggct ctcctgcaaa ccgcctcctc gccgcctcga ccatcgacag agaaccgtct
     1381 atagccagca cagatccctc gggcaacttc gcgagcaatg gctccgtcac ccggccagtc
     1441 ccgcaacccg cgtcgattgc agcctcgtcc ccacgcacct ccatacgctc gagaacctcg
     1501 accccccaac gggtctgcgg cgcggagagc ttctcatact cacctgcgtc ccactcgcgg
     1561 ggcaggatca caccttcttg ctttgtctca aaatatgaga cataatgacc gacatatgga
     1621 cagtatagaa gtagtttctt ccagaagcaa gagcggggtc ggggtgttgg acaaggctgt
     1681 ggctatcctg gacttccttt cgggggatgg cccagcgtcc ctgtcagagg tggtcgaggg
     1741 gaccaggtta ccccgtccga cggcgcacag gctcctatcg gcgctggaga cgcaccacct
     1801 tgtgtcgcgg cgggacggac gatacgtact cggcttgagg ctactagggt gggggaacag
     1861 ggctgctggg gtaggtctcg tggagcgggc gcgtcctgtg cttgagggat tgcgggatga
     1921 gagtggggag agcacgcagc tctacgtacg ggagggtgag cagagagtgt gcgtggccgc
     1981 tgtggagcgg gtgaccggtc ttagggacac cgtacccgtt ggggctgtca tgccgctctc
     2041 gcgaggatcg gcggggaagg tcttgctcgc ctgggctccg ggaggagtcg atggcaggcc
     2101 tgacgcggcc cagttgcttg ggatccgggt cagaggctgg gctgagagcg tggccgagcg
     2161 ggaggctggg gtggccagcg tcagcgcgcc ggtattcggg gaggatggga gactcagggc
     2221 ggccgtaagc gtcagcggtc cgatctcacg cctcggggag agtccgggcc agcggctttc
     2281 tcatctggtc ctgggagcag caagggagat agagagggcc acgggctcat agctcggcct
     2341 cagacgtgag gctcgcactc cacgcaagtc ggctttcgta gagcctatcc agggtcggct
     2401 ctttcgaggc gtggatggct gccttctcga aggtgatagt ggccgtacgg cggagctaga
     2461 gaactcgaaa aatcggtcaa catgacaccg cgcaatgggc gttttggtag tcgggtcggg
     2521 aaccagatgt cgcagaggtc tccaagaaga ttattcatct agggatgcac cgaatctact
     2581 atatagtgta gagtgccact gcaacatagc gaacataggc tgacatagga ggggattatg
     2641 ccggaggaga gtagagtcgc gggccccgcc cggcgaccag gcatggcagg caccgtacgg
     2701 cgcttgctga ggctcatcct tgtcgtggtc ctcctcgtcg gtgcgtcgca gggaaccact
     2761 ctcgccgcac ccgcctcgtg ggactatgtg gcactcggcg actctctggc cacaggcttc
     2821 ggtgccttca cgggctacgt gccccgctac gaagcgcaca tcgagaccga cactggggta
     2881 gcggtcacac gcacaaacct cggccggaac ggatggacca gttcccaact gctctctgcc
     2941 ctccgcagcg atccgacgtt ccggcgcgcc acccgtgagg cggagatcgt cacctggaat
     3001 atcggcggga acgatctcag ggcagcccgt aactcttaca aaaaaggaac ctgcggtggg
     3061 cgcgacaacc aggactgcct ccgggctagc gtggcgacac tcaagaccaa ctggaccgcc
     3121 atcacggcgg aggttctcga gttgcgttcg accgaaaata ccatggtgcg aacaatggat
     3181 atctacaacc cctatgtcag gaccgacata gcttccgaca cctggaagaa cgacggcggg
     3241 atgaacgact tccaggtttt caacagctat gtctacaagg ccaaccttca catcgccacc
     3301 acctcttacg cagcgggcgt atcctacgcc ggggtgcacc tggccttcaa cgggccgtac
     3361 ggctatgagg acccgaaatc caagggttat ctgagtttcg acggacttca tcctaacaac
     3421 accggacaca gagtcatagc cggcgagttg cagaagctgg gctacgctcc cttgagatag
     3481 gtcttgttgc tctggatctc cggtagatgg aggtcgggtc ggtactgagg aagcgcggga
     3541 cccagcacca tagcttcctg tccagatcgg atgacgcctt ctttcgggag taggatattg
     3601 gcgcggatcc accagcgctg tgattacttc agtagtcagc gactgcccga agaactcgga
     3661 tttgctgaag ggctgtccct gtagctcttg gtcgcccgat aggacgcgcg ttcgagtgac
     3721 gccatcatca agtacccaat gactgaaggc gtcaagacga tcatgtaccc cgtcacggac
     3781 ctcgccaacg ccaagacgct gtacggcgag cttctgggtg ttgagcccta cagggacgag
     3841 gcctactacg tcggcttcaa cattgaaggc cagaacattg gtctggatcc ccacggccac
     3901 agcgagggga tgacgggtcc cgtcgcctac cggcacgtta acgacatcga ggagcgtctt
     3961 caggcgcttc tcgacgctgg cgctgcggcg caccaagggg gcagggacgt cggcgggggc
     4021 aagttgctct cctccgtcaa agacacggac ggcaacgtca tcggcctcat ccaggcacta
     4081 tagttgcacg agagacgttc ttattgagca gttagagcca gccagtgtag caaggaggtg
     4141 tgttcaacac tgcgatgaca cggtacggca gggcggacac tgtcacaatg ggattccctc
     4201 gatcaatcaa ggagccatcg tgatgaaaac tccctacgag atcaggccta tcgggcacgt
     4261 cgtatcaccc ctcgtcgatc gcgagtctgc accgaagcag ggatctgaag gagcgccgga
     4321 cgcttggctg gtgttcgacc ccgacgtggc ggaaggcatc cgcgacctcg ccgtcggcac
     4381 cgacatcttc gtgctgacct ggcttcacca ggcacgccgc gacgtgcttg ccgttcaccc
     4441 gcgcgacgac ccccgaaacc ccgagaaggg tgtgttcagc accaggtctg aggacaggcc
     4501 gaatccgatc gggctgcacc gcgtacacat cgctgcgatc gacggacccc gggtgctcgt
     4561 gcgggatctc gaagccttcg acgagacgcc catcgtggac gttaagcccg tacttgccga
     4621 tgggaggtaa acgaccccgc gccctacact ctcaccagca tccgccgatc cttatggccg
     4681 agcacagagt gcccagatgc ggtcgcgcgg tacaggcggt ggccggcggt gttacgtcct
     4741 cccgtgtcca cccgctccat gcgcctgaga tctgcgagag aaggcgccga cgtcccggtc
     4801 gagctcggtg cccgaaagct cctcggcgat gccgacacgt acactccctg tcagtcccac
     4861 gccagcgtag aacgcccacc tgagcccctc gacgagaccc tcaagctcag acacgtttgt
     4921 tagccaggag agtcgtgttt ccttttcccg ggttgttcgc tcgtcatcgg gatcggattc
     4981 gctagaatga gatcatgga
//
LOCUS       DASTKM010000352         9524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_689695, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000352 DASTKM010000000
VERSION     DASTKM010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689695"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFQ10_13980"
     CDS             complement(<1..411)
                     /locus_tag="VFQ10_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966497.1"
                     /translation="MDFTVGDVRPTFPAELEVMPLERPPDATIRVPGSKSVTNRALII
                     AALANGNSRIVNPLFSDDSFWLMDALVRLGIDVHADGERGEVYVSGQSGEIYASGVDL
                     FVGNAGTVARFLPPFLALGRGPFTVDGVPRMRERP"
     gene            complement(435..1130)
                     /locus_tag="VFQ10_13985"
     CDS             complement(435..1130)
                     /locus_tag="VFQ10_13985"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9966498.1"
                     /translation="MRLQAFLARAGAAPSRRKAETPILAGRVTVNGETATLGVTVTPE
                     DEVKLDGRHVRLPEANIYLALNKPAGYLTTMNDDRGRKTVAHLMPEVPGLVPAGRLDA
                     ATTGLLILTNDGRVAHNITHPSSEIEKQYRLTLENPVPNAALTALAAGPTLEDGRMHA
                     PQITNLSRASETTSLDLTIHEGRNRIIRRACMALGLSLLSLHRTRVGPVTLGDLPEGE
                     YRALTASELEALR"
     gene            complement(1130..1750)
                     /locus_tag="VFQ10_13990"
     CDS             complement(1130..1750)
                     /locus_tag="VFQ10_13990"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET9966499.1"
                     /translation="MSEQERKLPSPRPAHLVEAVLLVSASPLGSSDLCSATGLSESEL
                     KRALACLRERYAPEASGIVLRDVAGGYLLSTNPACSGAVERFRREARAAPLSSAAHEV
                     LACALYLGPLTRAAVSRVRGVNSDAVVRSLIERGLLVEVGTDREAPGSPALLGVTDEF
                     LAATGVSSPDDFPPVDALVTQEELSRVRERLASSTTAPPESGGEPS"
     gene            complement(1747..2472)
                     /locus_tag="VFQ10_13995"
     CDS             complement(1747..2472)
                     /locus_tag="VFQ10_13995"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET9966500.1"
                     /translation="MTLEPPGRSLSDFTVELDVYSGPYEWLLALILKDELEIFEIPLR
                     ELVESYLFARRPDAANGLERDTDFAGSAAALVLLKSRTLSPAIDADTEGIEDEPLSPE
                     ELAERLTEYLKIRRAAEHLRNRFASNAGHYASGHTIPPRPGSLSIEPDRVSLAARQVF
                     SRVLEPPVRHLGPITVTLQELAGVIRTALAAGPISFERITSDMDRMNRAVAFAAALSL
                     AQEGSLTLAQIEPLGPLILEPSG"
     gene            complement(2469..3602)
                     /gene="ald"
                     /locus_tag="VFQ10_14000"
     CDS             complement(2469..3602)
                     /gene="ald"
                     /locus_tag="VFQ10_14000"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET9966501.1"
                     /translation="MRIGVPTERTQGESRVALAPHAVYELTRAGHRVFVEAGAGGASS
                     IADERYEEAGARLVEEAERVYADSEIVVKVYGPEVEEYDYLRENLVLISFLSLPVNLD
                     LMQALIDSRCIAFAMETIRDARGRLPVLTPMSEIAGRLTPQIGAHYLQRPFGGRGILL
                     GGSTGVRPGRVVILGAGIVGTGAARVASGLGAEVVIIDRDLDRLRQIEDARLPNTTTL
                     AASSLGIRDAVLRSDLLIGAVQVVGARTPALVDRETVSQMKDGSVIVDVDVDYGGCVA
                     TSRPTTLADPTFVEYGVTHYCVKNVPASVPVTATRALSNALLPYIRRLASVGLSDALA
                     ADEGLARGVVIAEGRVVDPVLARDTGFEYNQLSSVLPLHSEAR"
     gene            complement(3723..4226)
                     /locus_tag="VFQ10_14005"
     CDS             complement(3723..4226)
                     /locus_tag="VFQ10_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966502.1"
                     /translation="MCPTGDPAGTVATCTAGLLEFAATLSWNRVEDLPQVPTLPALPE
                     TARCSVVETDHPEAGRREILGEILALSERDLGSALQTVLRSLTTWAALSDAVLETRAN
                     RVTAPASAPATTLQALALDLRDAGFEVRFGPSWTPVPEGAVGLGVLGAEQELESFLDA
                     HPSWRTL"
     gene            complement(4280..5935)
                     /locus_tag="VFQ10_14010"
     CDS             complement(4280..5935)
                     /locus_tag="VFQ10_14010"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET9966503.1"
                     /translation="MSETKYIFVTGGVVSSIGKGTSAAALGMLLKSRGYRVVLQKFDP
                     YINVDPGTMNPYQHGEVFVTEDGAETDLDLGHYERFLDENLGRLSNVTTGSVYWEVIT
                     RERRGDYLGATVQVIPHITNEIKNRIGRLGQDKDIVITEIGGTVGDIESLPFLEAIRQ
                     FRNDVGRKNVLYVHVSYVPYIEAAGELKTKPTQHSAQRLREIGISPDVLICRADRPIS
                     DDIRKKIALFADTELDSVIAAEDAPNLYAIPLNLHEEGLDDLVLDKLGLSAPHADLGE
                     WRHLVSRMLEASQTVRVAIIGKYTKLQDAYLSVVEALAHAGGAHGLKVELDWVDSEQL
                     VDRASTEERLSGVDGVLVLPGFGHRGAEGKMEAARYARESGTPYLGLCLGMQIEVIEF
                     ARNVAGLEMANSTEFEQDTPHPVIDIMPDQVGVDMGGTMRLGRWPCQIVPGTLAEEVY
                     GSDLVHERHRHRYEVNNAYRDALADAGMVFSGTSPDGRLVEIAEIGDHPFYIGSQFHP
                     EFKSRPLRPHPLFYGFVGACGETLKQTADLKTEALRPEQGAAR"
     gene            complement(6446..7075)
                     /locus_tag="VFQ10_14015"
     CDS             complement(6446..7075)
                     /locus_tag="VFQ10_14015"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HET9966504.1"
                     /translation="MSLSVIPLAITMNAGPQIMSAIIFITASKPLKLSAYFLAGVVIA
                     VTAGVTITYILATTLGNSISLGDPSNSGSLGNIIQYLLVGLLVFLSIKSYVGRETIEP
                     PGWLGALQNANPRTAFMTGLLLLSVFPSDLVILVTVGVNLAQNNASLLGAVPFIVATI
                     LIAALPVLSYLLFRRRAQQIMPKVRDWMNTNSWLVNIIVYVVFIVLILA"
     gene            complement(7072..7410)
                     /locus_tag="VFQ10_14020"
     CDS             complement(7072..7410)
                     /locus_tag="VFQ10_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966505.1"
                     /translation="MDEDKPSCCTLRSENVMESGGKQMMHKRTGTVYRIVVRSELSNR
                     YAVAFEGMEMETKHGETILTGEVIDQPHLYGILSRINGLGLKLVSVQALPENAQWSTE
                     GKQESEEPEL"
     gene            complement(7452..>9524)
                     /locus_tag="VFQ10_14025"
     CDS             complement(7452..>9524)
                     /locus_tag="VFQ10_14025"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9966506.1"
                     /translation="WRQVEEAEVPFAWVSLDEQDNDPIRLWRHIVEALRQVVPQVEDF
                     GADVLVGLSAVGQRFVGITLPTLINELAELPHRVVLVLDDYQFLTEERSHETVAFLVE
                     HIPENIHLVISSRTDPPLHLGRLRARAQMNEIRTEQLAFTEEEADCLLNEKMALEIGP
                     DELSVLLERTEGWPAGIYLASLSLQNKEDKYAFIESFRGSNRYIVGLLGEEVLANLSE
                     EVRQFLLETSVLRTMTGPLCDAVTGRKDSAVLLRELARSNLFVVSLDEQGEWYRYHHL
                     FCELLLYELKSSQLDLVPTLRERASVWLEGAGFFEGAIRQSSEAGDYERVGLLIARHW
                     YGYVSAGQTATVERWLESLPEEEVTHDAAFALVKAWISALGGRREESARFRTLAESIP
                     YEGPLPDRTASVEAGVAILRASFGYDGVSSSLEAARRAAELEPGPTSPRAAMVRFALG
                     CSLYLSGDPAGARKSLEEALAMTEDDQRLVRVVALSFLSFIATDEGHLEEAESRARAA
                     RVLVEGLRPYEIPQTSLAPIALGRALAERGKLEEAQKELESGLFARRRLPGLSPWPTL
                     IGLLALAPLHAVRGDRAGARTVLAEAQTILERNPDAGIFPQLLERQERKLRARKAREG
                     QLDGELTERELDVLHLLVGELSTRQMAQNLYVAPSTVRTQVKSIYRKLGVSSRKKAVE
                     EAHVRGLI"
BASE COUNT         1700 a         2986 c         3014 g         1824 t
ORIGIN      
        1 cgggcgctcg cgcatcctgg gaactccgtc cacggtgaac gggcccctgc caagagcgag
       61 aaatggcggc aggaaacggg ccaccgtgcc cgcgttgccg acaaagaggt ctacgccaga
      121 tgcgtagatc tcaccgctct ggccgcttac gtagacttct cccctctcgc cgtcggcgtg
      181 tacgtcgatg ccgaggcgga cgagggcatc catgagccag aaggagtcgt ccgagaagag
      241 tgggttgacg atcctggaat ttccgttcgc gagggcggcg atgatgagtg cgcggttggt
      301 gacactcttg gaaccaggga ctcgtatcgt cgcatccggc ggcctttcca acggcattac
      361 ttcgagctcg gcggggaacg ttgggcgcac gtcccccacc gtgaaatcca cgccaggcac
      421 tcccatggcg tggttcaccg cagggcctcg agctcagaag ctgtgagggc gcggtactcg
      481 ccctcaggga ggtcgccgag ggttacggga cccacgcgcg tcctgtgcag ggagagcaaa
      541 gacagcccca aagccataca ggctctacgt attatgcggt tacggccctc gtggatagtc
      601 aagtcgaggc tggtcgtttc gctggcgcgg ctgaggttgg tgatctgggg ggcgtgcatc
      661 ctgccgtctt ctagggtggg acccgccgcc agtgctgtga gggcggcatt ggggaccggg
      721 ttttccaagg tgaggcgata ctgtttttcg atctccgacg aagggtgggt gatattgtgg
      781 gctactctgc cgtcgtttgt gaggatgagc agacccgtag tcgccgcgtc gaggcggcca
      841 gcgggtacta gtccaggaac ttcaggcatc aggtgggcga cggttttgcg gccacgatcg
      901 tcgttcatgg tggtcaagta tcccgctggt ttgttcaggg cgaggtagat gttggcttct
      961 gggagccgga cgtggcggcc gtcgagcttg acctcatctt cgggcgttac ggtcacgccc
     1021 agggtcgccg tctcgccatt taccgtcacg cggcccgcca gtataggcgt ctcggccttg
     1081 cggcgcgagg gtgccgcgcc ggcgcgggcg aggaacgcct gcaggcgcac tacgaaggct
     1141 cgccgccgga ctctggcgga gccgtagtag acgaagccag ccgttcgcgc acgcgggaga
     1201 gctcttcctg cgtcacgagg gcatcgacgg gcgggaagtc atctggggag gagacgccgg
     1261 tggcagcgag aaactcgtca gtcacgccaa ggagcgcggg tgagcctggg gcttcgcgat
     1321 ccgttccgac ttcgacaagg aggccgcgtt cgatcaggct gcgtactacc gcgtctgagt
     1381 ttaccccgcg cacgcgggag acggcggcgc gggtcagcgg tccgaggtaa agcgcgcacg
     1441 caaggacctc gtgggcagcg ctagagagcg gcgcggcacg ggcctccctg cgaaagcgct
     1501 cgacggcccc tgagcacgct ggattcgtcg agagcaggta gcctccggcg acatctcgca
     1561 gtacgatgcc tgaagcctcg ggggcgtagc gctcccgcaa gcaggcaagg gctcttttca
     1621 gctcgctttc agagaggccc gtcgccgagc agaggtctga ggagccgagc gggcttgcgc
     1681 tcaccaggag gacggcttcg accaggtgcg cgggccgtgg cgaggggagc ttccgttcct
     1741 gctcgctcaa cccgacggct ccaggatcag aggccccagg ggctctatct gggcgagcgt
     1801 gaggctaccc tcctgggcga gcgagagcgc ggcggcgaac gccacggccc tgttcatgcg
     1861 gtccatatcg ctagtgatcc tctcgaagga gattggtcct gcggccaggg ccgtgcggat
     1921 tactcctgcc agttcctgta gggtcaccgt gatcgggccg aggtgcctaa ccggcggctc
     1981 gaggacgcgg gagaacacct gtcgcgccgc cagcgacacg cggtctggct cgatgctgag
     2041 gcttcctggt ctgggaggga tggtgtgtcc tgacgcgtaa tggcccgcgt tcgaggcgaa
     2101 gcggttcctg aggtgttcgg ctgcgcgccg gatcttgagg tattcagtga gcctttcggc
     2161 gagttcctct ggcgagagag gctcgtcctc gatgccttcg gtatctgcgt cgatcgccgg
     2221 tgacagcgtg cgactcttca ggaggacgag ggctgcggcc gagccagcga agtccgtatc
     2281 gcgctccagg ccgttcgctg cgtcgggccg gcgggcgaac aggtaggatt cgaccagctc
     2341 gcgcagtggg atctcgaaga tctccagttc gtccttcagg atgagggcca gtagccactc
     2401 gtaaggcccc gagtacacgt cgagttcgac ggtaaagtcc gagagcgagc gccccggggg
     2461 ctccagggtc atcttgcctc cgagtgaagc ggcaagacgg aggagagctg attgtactca
     2521 aagcccgtgt cgcgggcgag gacggggtcg acgactcgcc cctcggcgat aacgacgccc
     2581 cttgccagcc cctcgtcggc cgccagggca tctgagagac cgacactagc gagcctgcga
     2641 atgtaaggca ggagggcgtt gctgagggcc ctcgtggccg tgacgggtac agatgccggc
     2701 acgttcttca cgcaataatg ggtaaccccg tactcgacga aggtagggtc ggcgagggtg
     2761 gtaggacggg aggtcgcgac acagcctccg taatccacgt ccacgtcgac gatcacgctc
     2821 ccatccttca tctgggagac agtctcgcgg tccacgagag ccggtgtacg ggcgcccacg
     2881 acttggaccg cccctataag aagatccgag cgcagcacgg catccctgat cccgaggcta
     2941 ctcgccgcca gggtcgtggt gttgggtagg cgggcatctt cgatctgcct gaggcggtcc
     3001 aggtccctat cgatgatgac cacctcggcc ccgaggccac tggccaccct cgcggccccg
     3061 gttccgacga tccccgcgcc gaggatgacc acgcgtccag gcctcacgcc cgtcgagccg
     3121 ccgaggagta ttccgcgacc cccgaagggt cgctgcagat agtgggcgcc tatctgcggg
     3181 gtgaggcggc ccgcgatctc gctcatcggc gtgagcacgg gcaggcggcc acgtgcgtcc
     3241 ctgatggtct ccatggcgaa agcgatgcaa cgggagtcga tgagggcctg catcaggtcc
     3301 aggttgaccg gcaacgagag gaaggagatg aggaccagat tctccctcag gtagtcgtat
     3361 tcttccactt ccggcccgta gaccttgacc acgatctcgg agtctgcgta gacccgctct
     3421 gcctcctcga cgaggcgtgc gcctgcctcc tcgtacctct cgtcggcgat gctcgacgcc
     3481 cccccggccc cggcctcgac gaagaccctg tgtcccgctc gggtcaactc gtatacggcg
     3541 tgtggggcca gcgcgaccct gctctccccc tgggtcctct cggttggcac cccgatcctc
     3601 atcgttcgct cccgaggagc tcggacaccg ggaccgaggg gaagactgcg taggaacact
     3661 gcgtactcat gttctcgaag ggtaccctgg atcttgcctg cgatccagcc gcgcaggggc
     3721 cctcagagag tcctccacga ggggtgcgca tccaggaaac tctctagttc ctgttcggcc
     3781 ccgaggactc cgaggcctac cgccccctcc ggcaccgggg tccacgatgg gccgaacctg
     3841 acttcgaagc cggcgtccct caggtcgagt gccagggcct gcaaggtggt ggccggggcc
     3901 gaggcagggg ctgtgacgcg gtttgccctg gtttcgagca cggcgtcgga gagcgctgcc
     3961 caggtcgtca gggagcgcaa gaccgtctgc agggccgagc cgaggtcccg ttcggagagg
     4021 gccagtatct cgccaaggat ctcacgtctg cccgcctctg ggtgatccgt ctcgacgacg
     4081 gaacatctcg cggtctcagg cagggcggga agcgtcggca cctgcggcag gtcttcgacc
     4141 ctgttccagg agagtgtcgc cgcgaactct aataggcccg ctgtgcaagt agcgacggtg
     4201 ccagcggggt cgcctgtggg gcacacggtg gtaagctgcc tttctgcact gacgaggccc
     4261 cgactttggc cgacctccct cagcgggccg ccccttgttc cggtctgaga gcttccgttt
     4321 tcaggtcggc cgtttgtttc agagtctctc cgcaggcgcc cacaaagccg tagaacaggg
     4381 ggtggggcct caaggggcgg ctcttgaact ccggatggaa ctggctcccg atatagaatg
     4441 ggtggtctcc tatctctgct atctcgacca acctcccgtc gggagaggtg ccagagaaga
     4501 ccatccctgc atcggcgagc gcgtccctat atgcgttgtt cacctcgtac cggtgccggt
     4561 gacgttcgtg gacgaggtcc gagccgtaga cctcctcggc gagcgttccg ggaacgatct
     4621 ggcagggcca ccgtccgaga cgcatcgtgc cgcccatatc gacgccgacc tggtcaggca
     4681 tgatgtctat aacggggtgc ggcgtatcct gctcgaactc ggtggagttc gccatctcga
     4741 ggccggcgac gttacgggcg aactcgatga cctctatctg catcccgagg cagagcccga
     4801 ggtaaggtgt cccgctctct cgcgcgtagc gcgccgcctc catcttaccc tcggcgcccc
     4861 tgtggccgaa gccaggcaac acgaggacgc catcgacgcc tgagagccgc tcctccgtag
     4921 aagccctgtc aacgagctgc tcggagtcga cccagtcgag ctccaccttc aggccgtggg
     4981 cgcctccggc gtgggccagg gcctcaacaa cggagaggta ggcgtcctgg agctttgtgt
     5041 acttacctat tatcgcgact cttaccgtct gactcgcctc cagcatgcgg gagacgaggt
     5101 ggcgccactc cccgaggtcg gcgtgcggcg cgctcagccc cagcttgtct agcaccaggt
     5161 cgtcgaggcc ttcctcgtgc aggttcaagg ggatagcgta caggttgggg gcgtcctcgg
     5221 ccgcgataac ggagtcgagc tccgtgtctg cgaagagcgc gatcttcttc cttatgtcat
     5281 cgcttatggg cctgtccgcc cggcagatga gtacgtcagg gcttatgccg atctcacgca
     5341 ggcgctgcgc agagtgctgg gtcggcttgg tcttcaactc gccggcagcc tcgatgtagg
     5401 gtacgtagga gacgtgaacg tagagcacgt tcttgcgccc cacgtcgttt cggaactgcc
     5461 ttatagcttc gaggaagggc aggctctcga tgtcgcctac ggtgcccccg atctcggtta
     5521 tgacgatgtc cttgtcctgg ccgagacgcc ctatcctgtt cttgatctca ttcgttatgt
     5581 gcgggattac ctgcacggtc gcccccaggt agtcgcccct tcgctcgcgg gttatgactt
     5641 cccagtagac gcttcccgtg gtcacgttgg agagcctgcc taggttctcg tcgagaaaac
     5701 gctcgtagtg accgagatcc aggtccgtct ccgccccgtc ctcggtgacg aagacctcgc
     5761 cgtgctggta agggttcatg gtccccgggt ccacattgat gtaagggtcg aacttctgga
     5821 gcacgaccct gtacccccgg ctcttgagca gcatccccag cgccgccgcg ctcgtcccct
     5881 tgcctatgga cgacaccacg ccacccgtca cgaagatata cttggtctca ctcatctaca
     5941 cccccaaaaa agagaacaaa cgtaacgccc ctaccttcac tattaatggg aaaaggggaa
     6001 gagtatccgg cggcggtgag gcgcgggatc atcgcttccc gcaaacctct actccggcac
     6061 acctgcatgg acctttatca tacgacttct ggcgccagaa tgaaaccgta gatcgcccag
     6121 cactaatcca cacagcgaca tctggcccaa agtgactact ctgccttctc cgccttcagg
     6181 ggcgtgtctg gacgcagcca cacagagacg tgctcaggct gcatcgtttc cctcacgacc
     6241 gctacaagct ccgcattcaa accgtccagg tccatctcgt tgcgtagcct ggcggagaac
     6301 ccttcgagag tctttctcgc gtcatacttc ttgcggtaga agcgtctgtc tatgaacgac
     6361 tgaacacgcc gtctcgaagg gacgacttcc gagcctccgt gctgggtgac gagtatcccg
     6421 ctgtccgaga tctctacgaa cgctctcagg cgaggatcag cacgataaac acgacgtaga
     6481 ctatgatgtt taccagccag ctgttggtgt tcatccagtc gcgcactttc ggcataattt
     6541 gctgggcgcg cctgcgaaac aggaggtaag agagcaccgg aagcgccgct ataaggatgg
     6601 tggccacgat gaaaggcacg gcaccaagaa gggaggcgtt gttctgtgcg aggttcaccc
     6661 cgaccgtcac cagaatgacg aggtcggaag ggaacaccga gagcagcagc agtccggtca
     6721 tgaacgccgt cctggggttg gcgttttgta gagcgcccag ccacccgggg ggttcgatag
     6781 tctcgcggcc cacgtagctc ttgatggaca ggaatacgag cagcccgacg agcaggtact
     6841 ggattatgtt gcctagcgat ccgctgttcg aggggtcgcc aagcgagatg ctgttgccta
     6901 gcgtggtggc gagaatgtaa gtgatcgtga cgcccgcggt gacggcgatc accacgcccg
     6961 ccaggaagta ggccgagagc ttcaggggct tcgaggcggt gataaagatg atcgccgaca
     7021 tgatctgcgg tcctgcattc atcgtaatgg caagggggat gacggaaagg ctcatagctc
     7081 tggttcctcc gactcttgct ttccttcggt actccattgg gcgttttctg gcaaggcctg
     7141 cacactcacc agcttgagcc ccaaaccgtt gatgcggctg aggatgccgt agaggtgcgg
     7201 ctggtcgatg acctccccag taaggatcgt ctctccatgt ttcgtctcca tctccatccc
     7261 ctcgaaggca acagcgtacc tgttgctgag ctcgcttcgc acaactatcc ggtagaccgt
     7321 acctgtacgc ttgtgcatca tctgcttacc gccgctctcc ataacgttct cagagcgtag
     7381 ggtacagcat gatggtttgt cttcatccac cagcacattt cccctagggg atttgtgggc
     7441 ttgtggggag gctagatgag ccccctgacg tgagcctcct ccacggcttt cttgcgagag
     7501 gagaccccga gcttgcggta gatggatttg acctgggttc ttaccgtgct cggggcgacg
     7561 tataggttct gcgccatctg ccgggtggag agctcaccga cgaggaggtg aagcacatcg
     7621 agctcacgct ccgtgagctc cccgtcgagc tgcccttccc gggctttgcg cgcgcgaagt
     7681 ttcctttcct ggcgctcgag cagctggggg aagatgccgg cgtcggggtt cctctccaga
     7741 atggtctgcg cctcggcgag caccgtccga gctcctgctc gatcgccacg cacggcgtgt
     7801 agcggcgcga gtgccagcaa cccgatgagg gtaggccagg ggctcaagcc aggcaacctt
     7861 cgtcgcgcaa aaagaccgct ctccaactcc ttctgggctt cctccagttt cccgcgttct
     7921 gcgagagcac gtcctagtgc gatgggtgcc aaactagtct ggggaatctc ataggggcgt
     7981 aacccctcaa ccagcacgcg cgccgcacgc gcgcgtgact cggcttcctc caggtgccct
     8041 tcgtcggtag ctatgaatga caagaaggat agggcaacca cccgcaccaa gcgctggtca
     8101 tcttctgtca tggcaagcgc ttcttcgagc gactttcgag cccccgctgg gtccccagaa
     8161 aggtataggc tgcatcccag cgcgaagcgc accatcgcgg cccgtggcga ggttgggcca
     8221 ggctccagct ctgcggcgcg ccgggcagcc tcaagagagc tgctgacgcc atcgtaaccg
     8281 aagctggccc ggaggatagc cacgcctgct tcgaccgatg cagttctgtc gggaagcggg
     8341 ccttcgtacg gtatgctctc ggcgagcgtc cggaatctcg cgctctcttc gcgccggcca
     8401 ccgagggcag agatccacgc cttcaccagg gcgaatgctg catcatgggt gacctcctct
     8461 tcaggtaacg actcgagcca cctttccaca gtcgccgtct ggccggcgga cacgtatcca
     8521 taccaatggc gcgcgatcaa tagccccaca cgctcatagt caccggcttc gctggactgc
     8581 ctaatcgccc cctcgaagaa tcccgcgccc tctaaccaca cgctcgcccg ttcgcgcagg
     8641 gtaggcacca gatcgagttg gctactcttg agctcgtaga gcagcaactc acagaagaga
     8701 tggtggtatc ggtaccactc gccctgctca tcaagagaaa ccacgaacag gttggagcgg
     8761 gcgagctcgc gcaggagcac agcagaatcc ttcctgcccg tcaccgcgtc gcagagcgga
     8821 cccgtcatcg tcctcaggac cgaggtctcg agtagaaact gcctcacttc ctcgctgagg
     8881 ttcgccagaa cctcctctcc caacagaccg acgatgtagc ggttggagcc cctgaaggac
     8941 tcgatgaagg catacttgtc ctccttgttc tgcaaggaca gggaggccag gtagatcccc
     9001 gcaggccacc cttctgtacg ctcgagcaat acggagagct catcagggcc gatctccaga
     9061 gccatcttct cgttcaacag gcagtcagct tcctcctctg tgaaggcgag ttgctcggtg
     9121 cgtatctcgt tcatctgtgc cctggccctc agccgcccca ggtgcaatgg cgggtcggtc
     9181 cggctcgaga tgacgaggtg gatgttctca gggatgtgtt ccaccagaaa ggccaccgtt
     9241 tcatgagagc gctcctcggt taggaactgg tagtcgtcga gtaccagcac aacccgatgt
     9301 ggcaactcgg cgagctcgtt tatgagcgtg ggaagcgtaa ttccgacgaa cctctgcccc
     9361 acggcgctca gtccaacaag gacatcggct ccgaagtcct ccacttgtgg cacaacctgg
     9421 cgcaaagcct caacaatgtg tctccacagc cggatcggat cgttgtcctg ctcgtcgagg
     9481 gagacccagg cgaagggcac ctcggcttct tctacttgac gcca
//
LOCUS       DASTKM010000353         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1219738, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000353 DASTKM010000000
VERSION     DASTKM010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219738"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFQ10_14030"
     CDS             complement(<1..473)
                     /locus_tag="VFQ10_14030"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET9966507.1"
                     /translation="MADAKRQPLSRRRILEAAVRFVDREGLEALSMRKLGSELGVEAM
                     SLYNHVPNKGALLDGMVEVLLGELEVPPEDEGWERRIREAYRAFRRLAHEHPNVFPLL
                     VVRPPDTMDGIWLVEEFLKTLREAGFDPQTALYAFRALSSYASGYAMAEIRGFAM"
     gene            complement(705..986)
                     /locus_tag="VFQ10_14035"
     CDS             complement(705..986)
                     /locus_tag="VFQ10_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966508.1"
                     /translation="MEGPVTQATGHNNTIVSVYEDRVELRTGWQSQNVERVGLRDVAV
                     IGIKGLVNVTLTIETNTGRVYQLNRMALPDARRIKNAFEQQKRRAGLYD"
     gene            complement(1112..>2414)
                     /locus_tag="VFQ10_14040"
     CDS             complement(1112..>2414)
                     /locus_tag="VFQ10_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET9966509.1"
                     /translation="TMWVIVAACLVLFMQAGFAMLEVGFSRMKNVGSVVAKILVNLAI
                     AALLFWAVGFALAFGSGNALVGTEGWFLAVPAGQVNDVYAGLSWTQVPLSAKFLFQVA
                     FCAVSLAIVWGTMLERTKFAVYVVFAIVFAGLIYPMVGHWIWGGGWLTELGMQDFAGS
                     TVVHLSGAMAALAGTLLLGPRLGKYDDDGRPVNIPGHNMPLAVLGVLILWIGWWGFNP
                     GSTMAAVTDIADIALTTNLAAAAGVLGAMGMSYLYRHNVDVGMGGNGAIAALVAITAP
                     CAFVAPWASIVIGFVAGIIMYATLVYVDKIGVDDPLGAIAAHGMGGIWGTLSCGLFTT
                     PALAAVGQPGLFYGGGLYQLGIQALGIVAAGGFVFLASLAVFATLKATIGIRVKPEQE
                     LDGLDIHEHGVFGYPDLVATDYQNGNGKISDPMEGVPEGTA"
BASE COUNT          493 a          758 c          740 g          423 t
ORIGIN      
        1 tccatcgcaa aaccgcggat ctcggccatc gcgtagccgg aagcgtagct ggagagagcg
       61 cggaaggcgt acaacgccgt ctgggggtcg aagcccgcct cccttagcgt cttgaggaac
      121 tcctcgacca gccagatccc atccatcgta tccggcggcc ggacgacgag caagggaaag
      181 acgttgggat gctcgtgcgc tagccgccgg aaagcccggt aagcctccct gatccgtctc
      241 tcccagccct cgtcctcagg cggcacctcc aactcaccaa gcaagacctc gaccatccca
      301 tcaagcaaag cgcccttgtt cgggacgtgg ttgtagagcg acatcgcctc gaccccaagc
      361 tcactcccta gcttccgcat actgagagcc tccaacccct cccgatccac aaaccgtacc
      421 gccgcctcta gtatccgtcg ccggctcaac ggctgccgct ttgcatcggc cattgaaaac
      481 ctccttctgc ttacaaccct tgacagctta cagtataagc gtcgcattta cgacgtaagt
      541 attattgaat tgtaaagttc gaattgtgaa ttgagaaagg gctacatgat ggaccactcc
      601 aaattccaga ttcaagaggc tacgagcgac gggcgagagc ggaaacagca agctgaaatg
      661 ctgacgagcg cccgacgggg cgcgtgctga cttgctgaaa tgctctaatc gtacaggccg
      721 gccctgcgct tctgctgttc gaaggcgttc ttgatcctgc gcgcgtctgg gagggccatg
      781 cggttcagtt ggtagacgcg acccgtattg gtctctatcg tcagggtgac gttgacgagg
      841 ccctttatgc cgatcacggc gacgtcgcgc aggcctacgc gctcgacgtt ctgggactgc
      901 cagccggtcc tcagttcgac ccggtcctcg tagacgctga cgatggtgtt attgtggcca
      961 gtggcttgtg ttacaggtcc ttccaagctg tcctccgtgc cgcttgcggt ccgcctctag
     1021 caccagcata caagacggcg ttaccgattt tggacggaca tgaaaaaaca ccggccggag
     1081 gagatccggc cggtgccaac gcgtttctgc tctaggctgt accttctggc acgccttcca
     1141 taggatcgga gatcttcccg ttcccgtttt ggtaatcggt tgcgacgagg tcggggtagc
     1201 cgaagactcc gtgctcgtgg atatcgagcc cgtcgagctc ctgctcgggc ttgaccctga
     1261 tgccgatggt ggccttgagc gtcgcgaaga cggcgagcga ggcgaggaag acgaagcccc
     1321 ctgctgcgac gataccgaga gcctgaatac cgagttggta gaggccgccg ccgtagaaca
     1381 ggccgggctg gccgacagct gcgagggccg gggtggtgaa caggccgcag gagagcgtgc
     1441 cccagatacc gcccatgccg tgcgccgcga tagcgccgag cgggtcgtcg accccgatct
     1501 tatccacgta gacgagggtg gcgtacatta ttatgccggc gacgaagcct atcacgatcg
     1561 aggcccaagg cgcgacgaaa gcgcagggcg cggtgattgc gacgagggcc gctattgcgc
     1621 cgtttcctcc catcccgacg tccacgttgt gcctgtacag gtagctcatg cccatcgcgc
     1681 cgaggacccc ggcggctgcg gcaaggttcg tcgtcagggc gatgtcggcg atgtcggtaa
     1741 ctgcggccat cgtcgatcct gggttgaagc cccaccaccc gatccagagt atgaggacgc
     1801 cgaggacggc gagcggcatg ttgtgaccag ggatattcac ggggcggccg tcgtcgtcgt
     1861 acttgccgag gcgcggaccg aggagcagag tgccggcgag cgccgccatc gcgccagaga
     1921 ggtggacgac cgtcgagcct gcgaagtcct gcatgccgag ttcggtcagc cagccgccgc
     1981 cccagatcca gtgacccacc atagggtaga tcagaccagc gaagacgatg gcgaagacga
     2041 cgtagaccgc gaacttggtg cgctcgagca tcgtgcccca cacgatggcc agtgagacgg
     2101 cgcagaacgc gacctggaag aggaacttgg ccgagagcgg gacctgcgtc caggaaagac
     2161 cggcgtagac atcgttgacc tgtccggccg gaaccgcgag gaaccagcct tcggtgccaa
     2221 cgagcgcgtt acccgaaccg aaggccagcg cgaacccgac cgcccagaac aaaagggcgg
     2281 cgatggcgag gtttaccagg atcttcgcca ccacgctccc cacgttcttc atccgggaga
     2341 agccgacctc gagcatagcg aatcccgcct gcatgaaaag gaccagacac gccgctacga
     2401 tcacccacat cgtg
//
LOCUS       DASTKM010000354        12116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1569803, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000354 DASTKM010000000
VERSION     DASTKM010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12116
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569803"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..822)
                     /locus_tag="VFQ10_14045"
     CDS             complement(169..822)
                     /locus_tag="VFQ10_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9966510.1"
                     /translation="MRVLVAGAHGKTARRLVRMLAEDGHEVRGLVRKEEQTGDVGADG
                     AEPVVVDLEAEEVGGGIGRAVEGCDAIVFAAGAGPGSGAERKETMDYGGAAKLVEAAE
                     ERGVRRYLMLSSIGADDPEGGSEPMRPYLRAKSRADERLKSSSLDYTIIRPGSLTEDE
                     GTGTIEAAEKLGRRDEIPREDVASTFAHALENENTYHKTFVTPVDASVLTLTVTTVK"
     gene            complement(1291..1443)
                     /locus_tag="VFQ10_14050"
     CDS             complement(1291..1443)
                     /locus_tag="VFQ10_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966511.1"
                     /translation="MRIPPTGYLTLPCGELVRVIVWARTGIFLVLVVGIATGIGSVVA
                     AMRWEV"
     gene            complement(1810..2022)
                     /locus_tag="VFQ10_14055"
     CDS             complement(1810..2022)
                     /locus_tag="VFQ10_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966512.1"
                     /translation="MGDRRKRLAREDLEAMRQEFVVGEQAGSLDDDLHQIRRSARLGV
                     SPEDWARARKLSPAYARALRRYLDQG"
     gene            2244..3461
                     /locus_tag="VFQ10_14060"
     CDS             2244..3461
                     /locus_tag="VFQ10_14060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET9966513.1"
                     /translation="MRVRMTPVKGPATSIAFLILLLLSPVFSLDRAVAQEKTPEAPPG
                     APKLNAGSWTLIDADTGLYLAGKNPDEQVPIASTTKIMVALVALDEGVDFDEQVTVSE
                     DAASYAGSIYSNIGLYPYDRVSVRDLLTAALVPSGTDAVYALAEYLGDGSVDEFVAKM
                     NDKAKELGLENTHFENPAGIDARGNYSSAADLATITREAVKYPEFREIVDMPEATIST
                     QDREIDVVNTNLLVLPNSGYDYGPATGAKTGTSPQAGPCLVASAKSDDESYIAVVLDA
                     AEDLQRYEAATTALEYGFGEYEREPLVEHQEPYADLELPYRRGETVKLVAAKNVSALA
                     GPGLKVEQRTKNEEAPPSAEAGRKLGTVTVSVDGRNAGSSPLVVQKGYEEASLWQMVT
                     YWAGGLKRWILSR"
     gene            3617..4345
                     /locus_tag="VFQ10_14065"
     CDS             3617..4345
                     /locus_tag="VFQ10_14065"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HET9966514.1"
                     /translation="MAGLGVGQKITYFELPDQQDYPWSLSGQLEMGPVMLIFYRGDWS
                     PYCNGQLARYAREYEEFERRGAQIAGISVDPPSNNAKMVGKLLLPFPLLSDPEGELAR
                     LCGLWNAEEGVAVPSIVVIDQSGEVRYLYSGTDFADRPRDEEVLAALDGLDSGIERLT
                     GGPEFRVGATQASESSVRPGRRAMEFEQLIPYYRGVYYTTEVLKARFGGWGRAGKQAL
                     EELDAFQQMVRSYIEALEETIRLH"
     gene            4370..5392
                     /locus_tag="VFQ10_14070"
     CDS             4370..5392
                     /locus_tag="VFQ10_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9966515.1"
                     /translation="MELFLDKSTGRLRALWRLAIQYWAYWELVQILLSLLAVTWLLEG
                     SDGRTASSGPDASVIPYSPVLPLVSAVTSLLAAILTVWLAGRFLDRRPFSAFGFRLGA
                     GWWLDLLFGMALGALLMTAVFLVELGLGWVTVTGAFETYGTGAPFAVAMLIPTAAFLC
                     IGFYEETIFRGYQLKNAAEGLNYPALGPGGAILLAWLLSSVFFGVLHAGNPNATPIST
                     LNIVLAGLMLGFGYVLSGELAIPIGLHITWNLFQGAIYGFPVSGLEPSGATLLATEQS
                     GPELWTGGSFGPEGGLLIPAVMLLGMSLVALWTRLRTGKISLHTPIAESPSRDRQHIP
                     PQRHRA"
     gene            5608..6507
                     /locus_tag="VFQ10_14075"
     CDS             5608..6507
                     /locus_tag="VFQ10_14075"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002827666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9966516.1"
                     /translation="MSEDQHRQQDPTQQYPQPEYPEQDQRDQHPGLEQEMQPEPDYGV
                     DTYRGSGKLEGKKAVITGGDSGIGRAVALAFAREGADVVISYLVEEEADAQEAAQAVE
                     DSGRRAVRVPKDVADEANCQDLIQKAVDEFGRIDILVNNAAHQMTVEGIADVSTDLLD
                     RTFKTNIYAMFWLVKAALPHMPEGGSIINVSSIQAYQPSPTLLPYSSTKGAIITFTKG
                     LAQEVVQYGLRANTVAPGPVWTPIIPASMPGDTVSSFGGTSPMGRPAQPVELAPAFVF
                     LASQEASYVNGETLGVTGGQPLP"
     gene            6597..7817
                     /locus_tag="VFQ10_14080"
     CDS             6597..7817
                     /locus_tag="VFQ10_14080"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET9966517.1"
                     /translation="MVQKYGGSSVATAEHIRAVAERVKRVHDSGHGLVVTVSAMGKQT
                     DRLLRLAGEVSRDPSPREIDQLLSTGELQSVALLAMALHDRGVAATSLTGPQAGMKTT
                     GRYGSGLISGIQPDRLRGLLDEGQVAIVAGFQGVNALGDIMTLGRGGSDTTAVALAAA
                     LGAERCEVFTDVDGVYTADPRIVAGARRIPVILSSEMAEMSWRGAKVMHPRAVELGAL
                     HGVEIHVKSSFEEGPGTIITGGNGLEHLETRETVAGIVHDLDVSRITLTGIKTGPGTM
                     SRVFSPLAEAGISVDVIVESAPKEGTSDVAFTVRRGDFSQALRIAGAVADSLNGRIEG
                     EEQLGKVSVVGTGMLNRPGYAARMFATLGEAGIPIRLVSTSEIQVTCVLPAGRVEEAV
                     RVLHRDFELEERDV"
     gene            7810..8703
                     /gene="dapA"
                     /locus_tag="VFQ10_14085"
     CDS             7810..8703
                     /gene="dapA"
                     /locus_tag="VFQ10_14085"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET9966518.1"
                     /translation="MFSGAFTALVTPFRNGEVDVEALEGMVEFQIHNGIHGLVPCGTT
                     GETPAMTEEEDRLVISTVVRVAQGRVPVIAGSGSNSTDMAIKYTKMAEEEGADGSLQV
                     APYYNKPTQEGLYRHFATIAESTDLPIILYNIPGRTAVTISAETISSLSEIPNIVGVK
                     DSTLSMNMVSDIKSLCGEEFAILSGDDPMTLPLMALGGHGVISVASNVAPAVVSDMVR
                     TLLEGDWERGRELHYELLPLFRALFVETNPIPVKTAASILGLCSDEMRLPMIPLQGEN
                     LDTLRRVMEESNHLLPTPEEV"
     gene            8700..9479
                     /gene="dapB"
                     /locus_tag="VFQ10_14090"
     CDS             8700..9479
                     /gene="dapB"
                     /locus_tag="VFQ10_14090"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET9966519.1"
                     /translation="MTKIAVIGAAGRMGREICRASLETEGIELEGGTLRAGAAELGSD
                     LGELCGLGKTSVTATEDPPGSAEVLIEFTTPEATVEHLSYQRPHVIGTTGLSAEQLAR
                     VEEASDSVPIVRAPNMSVGVNLLREVVREISAKLGEGYDIEVVEAHHRNKKDAPSGTA
                     LFLARAAAEGRKRNLDEVVVYGREGLAPRQEGEIGIHALRGGAVVGEHRLVFYSGGEE
                     LEVVHRVLSRRTFADGALRAARFAANAKPGLYSMADVLGDF"
     gene            9489..11354
                     /locus_tag="VFQ10_14095"
     CDS             9489..11354
                     /locus_tag="VFQ10_14095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9966520.1"
                     /translation="MTDASNVPLIPLKVLFGNPEKVAPRLSPDGERLAYLAPKDGVLN
                     VWVGPVGRPAGGVDFEPVTDDRKRGIRVFFWAENNTHIVYLQDEGGDENWRVHAVDAS
                     TKQDRDLTPFDDVQAQIVDSSRRFPDALLVALNRENQELHDVYRLALSTGDLELVARN
                     PGNAVRWVVDAHLRVRGAMAATPDGGFDLLVREGADEDADWRKLLGWGKEDALSSGPV
                     GFTESGVKMYLIDSRGANASRLVLLDLENGSTKTLVEDPRYDVGRVVKHPETHEAQAA
                     AVERARTEWVVLDDDIREDFEVIGGLGRGDFGVTSRDRADENWLVAFTADQGGASYYA
                     YDRKNKRGEHLFDARPDLAEYTLARMEPVSFASRDGLTIEGYLTLPPGTSGGPMPMIL
                     NVHGGPWVRDGWGYDPEAQWFANRGYACLQVNYRGSTGYGKEFLNAGNKEWGGTMHDD
                     LVDAVGWAVQRGVADPERVAIYGGSYGGYAALAGATFTPDLFRCAVDIVGPSSLITLI
                     NSIPPYWKPLLATFHERVGNPDTEGDFLESRSPLFFVDRIRIPMLIAQGANDPRVKQA
                     ESEQIVAAMKEKDIDYEYLLFEDEGHGFARPENRLKFYAAAERFLAKHLGGRAQE"
     gene            complement(11380..11544)
                     /locus_tag="VFQ10_14100"
     CDS             complement(11380..11544)
                     /locus_tag="VFQ10_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966521.1"
                     /translation="MEDRGEVCLAEEVERFRRLGLSDGEIASKLGVDASWVATVVSSG
                     EEETALESAD"
     gene            complement(11597..11878)
                     /locus_tag="VFQ10_14105"
     CDS             complement(11597..11878)
                     /locus_tag="VFQ10_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9966522.1"
                     /translation="MRRDRAKYRRRRLGALFVAAALAVALYVGGVAGAEPQPVHYTVA
                     PGDTLWGIAIEHTAVWEDPRPKVEAIREANDLSGYEIYPGMSLKVPSTV"
BASE COUNT         2355 a         3927 c         3766 g         2068 t
ORIGIN      
        1 cgctcttagc ggccttccac agccgaaaca cgctgttcat ctctgcgcga cgatcttctg
       61 acatcgtcac taggttacat ggagcgtgct ctatctctgg tcgggcagac ccactccgcg
      121 cctcctgtct tctgagccta gtgacacttt acctggctat gcacctgctc atttaacggt
      181 cgtcaccgtg agagtcagta ccgatgcgtc aaccggtgta acgaacgtct tgtggtatgt
      241 gttctcgttt tcgagcgcat gggcgaaggt actggcaacg tcttcgcggg ggatctcatc
      301 ccgcctccct agcttctcgg ccgcctctat ggtgcccgtg ccctcatcct cggtcaggct
      361 gccagggcgg atgatggtat agtcgagact actgctcttc agacgttcgt cagcgcggga
      421 cttggcgcgg agataaggtc gcatcggctc agagcccccc tcagggtcgt ccgcgccaat
      481 agagctgagc atcaagtagc ggcgcacccc acgttcctcc gcggcctcga ccagcttggc
      541 ggccccgccg tagtccatcg tctccttgcg ctcggccccg ctccccggtc cggcgccggc
      601 ggcgaacacg atggcgtcgc acccctcgac cgccctcccg atcccccctc cgacctcctc
      661 ggcttcgagg tcgaccacca caggctcggc tccgtccgcc ccgacatcgc ctgtttgctc
      721 ctccttgcgg acgagtcccc tgacctcgtg gccatcttcg gccagcatcc gtaccagacg
      781 cctcgccgtc tttccgtgcg cgccagcgac caaaaccctc acgaaactac ctccttcgaa
      841 ccaggacacc ctgcagttta cagcggatcg aggaagggtc gacccttcct cgatccgcgc
      901 tggtcaccat ttcagggaga cgctgtcgcg gacgcagagg gtgaagctgt tggggatgca
      961 gttggggacg ccgtcgggga cgatgtcgga gatgccgttg ggccggtcgt ctccagaagc
     1021 gtggtacctg gggtcgtcgc cggtgatgta ctgggttgcg gccccgcagg atcggtcggt
     1081 gtttcgtaga atggggacag cagcgcggac aacacaacgg caacagccaa agcggcaacg
     1141 gtgcacacgg gcgcgagaac gatggccaga ggcgcgcgca gccactccga cacccttccg
     1201 cgcagcatgt agtagaggag cccggcgaaa acgacgagca cggatatcaa gagccacaaa
     1261 gggtatccaa gatcgagcac acccattctt ctaaacctcc caacgcatcg cagcgactac
     1321 agaaccaatt ccggtggcga tgccgacgac gagtacgagg aagatccctg tacgggccca
     1381 gactatgacg cggacaagct ccccacaggg cagcgtcagg tatcccgttg gcgggatcct
     1441 catcgggtct cctctccggt gctttacagg gcgtagccta gaggacaaac aaaacgtccc
     1501 tgcaacgatc gtattctagt acggaagtcg ttctcgcgag tctagagagc gaggaagagc
     1561 ctggatccat cagggaccct ggcccgatac ggcgtgctgg cgggggcaac ggagtaggac
     1621 cgggccggta aggaggggga aggtaccgaa ggcccgatcc tatgggtaat gtcgccgggc
     1681 gtcggggtca tgagagaggg atcctcgccc ggtcttgcac tactctacga tatgaggagc
     1741 aggcggcgca tcccccaaat gagtgatatt tgccagatcc gaaaagatcg aggcgttcac
     1801 gaccggactc tatccttgat ccaggtacct ccgaagcgcc ctggcgtagg ccggcgacag
     1861 cttccgcgcc ctggcccagt cttcggggct cacgccaagc ctggcactgc ggcggatctg
     1921 atggaggtcg tcgtccaggc tgccggcctg ctcgccgact acgaactcct ggcgcatggc
     1981 ctccaagtcc tctctggcca acctcttccg acggtctccc atgcatcctc ctctcgccga
     2041 aagagcacgg accaccctaa gcgtaacccc accccatcgc agcggcaatg gttgatgctg
     2101 tgaatcttct ccataatccc acaagatcga cggtcttcgg ggtggtaagg gaaggtcctc
     2161 tcgtgcaaaa tactctcgtc cgccgccggc ggcatcgttg tagggggagc acagccggtt
     2221 atcgaggtag aggagagcaa cgggtgcgag taagaatgac cccagtcaaa ggacctgcga
     2281 cctctatagc gttcctgatc ttgctgctcc tttcaccagt gttctcactc gatcgggccg
     2341 tggcccagga gaagacgccc gaggcgcctc ccggggcccc taagctgaac gccggttcct
     2401 ggacgctcat cgacgccgac acaggcctct acctggcggg caagaacccg gacgagcagg
     2461 tccctatagc ctcgacgacc aagatcatgg tcgccctggt ggcactggat gagggtgttg
     2521 acttcgacga acaagtaact gtctcggagg acgcagcctc ctacgccggc tccatctaca
     2581 gcaacatcgg cctctacccc tacgaccgcg tgagcgtcag ggacctcctc acggcggctc
     2641 tggtaccttc cggcaccgac gcggtctacg ccctggccga gtatctgggg gacggcagcg
     2701 tggacgagtt tgtcgccaag atgaacgata aggcgaagga gttgggcctg gagaacaccc
     2761 atttcgagaa ccctgccggc atcgacgccc gcggtaacta ctcgagcgcc gcagacctcg
     2821 cgacgataac cagggaggcg gtgaagtatc ccgagttccg cgagatcgtc gacatgcctg
     2881 aagccaccat ttcgacgcag gacagggaga tagacgtcgt caacaccaac ctgctcgtct
     2941 tacccaattc aggctacgac tacgggccag cgacgggcgc caagacgggc acctctcccc
     3001 aggccgggcc ttgcctcgtg gcctccgcaa agagcgacga cgagtcctac atcgcagtgg
     3061 tgctcgacgc cgcagaggac ctgcaacgtt atgaggcagc cactacggcg ctcgagtacg
     3121 gcttcggcga gtacgaacgc gagcctctcg tcgaacacca agaaccttac gcggacctgg
     3181 aactgcccta ccgcaggggc gagacggtga agctcgtcgc cgcaaagaac gtatccgcgc
     3241 tcgccggccc aggcttgaag gtcgagcagc gcacgaagaa cgaggaggcg ccgccctccg
     3301 ccgaagccgg gcgcaagcta ggcaccgtca cggtttcggt ggacggccgc aacgcaggta
     3361 gcagccccct ggtcgtccag aaaggctacg aagaagcctc cctgtggcag atggtaacgt
     3421 actgggccgg gggactgaag cgctggatcc tttcccgctg agcaccagcc ggggccggcg
     3481 tgtgcagacc agccgacttt gccttcgcta ctgcgggtgg aggcggtaac caggagggcc
     3541 ggtttcactt ccgcgagaag tcgcgtcctc catgctatat tcgcaccctg gaggattaac
     3601 gagggaggag catcacgtgg ccgggttggg tgtcgggcaa aagatcacgt acttcgagtt
     3661 gccggatcag caagactatc cctggagcct ctcgggccag ttggagatgg gacctgtgat
     3721 gctgatcttc tacaggggcg actggtctcc ctactgcaac gggcaactgg cccgctacgc
     3781 cagggagtat gaagagttcg agaggcgggg ggcgcagata gcaggcatca gcgtcgaccc
     3841 cccatccaac aacgcgaaga tggtcgggaa actcttgctt cctttccccc tccttagtga
     3901 cccagaggga gagctcgccc gtctgtgcgg cctgtggaat gcagaggagg gcgtggccgt
     3961 gccttccatc gtcgtcatag atcagtcggg ggaggtacgc tacctctact caggcacaga
     4021 ctttgcagac aggccgcgag acgaggaggt cctcgctgcc ctcgacgggc tggacagcgg
     4081 gatagaacgc ctcacaggcg gaccagagtt ccgggtagga gcgacgcagg cgagcgagag
     4141 cagcgtccgc ccagggcggc gcgccatgga attcgagcag ctcatcccct actatcgggg
     4201 cgtctactac accactgagg tcctcaaagc gaggttcgga gggtggggtc gcgccggaaa
     4261 gcaggcgctc gaggaactgg acgccttcca gcagatggta cgctcttaca tcgaggccct
     4321 ggaagagacc atcaggctgc attagcacgc tcaggcaagc ggaggctgct tggaactgtt
     4381 tctcgacaag agtactggac ggctcagggc gctgtggcgg ttggcgatcc aatactgggc
     4441 ctactgggag cttgtccaga tcctcctgag cttgctggcg gtcacgtggc tacttgaggg
     4501 ttctgacggg cggaccgcat ccagcggacc cgacgcctcg gtcatccctt actcccccgt
     4561 tctaccgctt gtaagtgctg taaccagcct attggcagcc atcctgacgg tgtggcttgc
     4621 gggacgcttc ctcgacagaa gaccgttctc ggcgttcggg ttccgtctcg gcgccggatg
     4681 gtggctcgac ctgctcttcg ggatggccct cggcgcgctg ctcatgacgg cggtcttcct
     4741 cgtcgagctt gggcttggtt gggtcacggt aacaggcgct ttcgagacct acgggaccgg
     4801 cgctcctttc gccgtcgcca tgctcatccc caccgccgcg ttcctgtgca tcggcttcta
     4861 cgaggagacg atcttccgag gttaccagct caagaacgcc gccgagggcc tgaactaccc
     4921 ggccctcggt cccggcggcg ccatcctgct cgcctggctc ctctcctcgg tcttcttcgg
     4981 cgtgctacac gccggcaacc cgaacgccac gcccataagc acgctcaaca tcgtcctcgc
     5041 cggcttgatg ctcggctttg gctacgtgct ctccggggaa ctggcgatcc ccataggtct
     5101 gcatatcacc tggaacctct tccagggcgc aatctacggc ttccctgtca gcggcttaga
     5161 accttccggc gctactctcc tggccacaga acagagcggc ccggaactct ggaccggtgg
     5221 ctcgttcggt cccgaaggtg gcctgctcat ccccgccgtc atgctcctgg gaatgtctct
     5281 cgtcgcgctg tggacccgtc tgcgcaccgg caagatctca ctccacaccc ccatcgctga
     5341 aagcccgtcc cgcgaccgtc aacacatccc gccgcaacgg catcgcgcgt aaggctcgag
     5401 cctcacgcac gactcaccaa cagcaagtca gcacttcggg ctcacgcgtc ggtgatgatc
     5461 ctgcagttca ccctaccgaa cagaggatct tcccccgccc gagacaggat gccgctcgga
     5521 ggcgcgcctt cacggtttga tgcggcaagt aaaagggcaa atgaccgcaa gaaacgagta
     5581 gatcagccgc gcggaaggaa gatcaggatg tcagaagacc agcacaggca gcaggacccg
     5641 acgcagcagt atccgcaacc cgagtatccc gaacaggacc agcgggacca gcaccccggc
     5701 ctggaacagg agatgcaacc cgagccagac tacggtgtcg atacctaccg cggaagtggc
     5761 aagctcgaag gcaagaaggc ggtcataacg ggcggtgact cggggatagg gcgggcggtt
     5821 gcgctggcct tcgcccgcga gggtgccgac gtcgtgatct cctacctagt agaggaggag
     5881 gccgacgccc aggaggcagc gcaggccgtc gaagactcgg gccgcagggc cgtcagggtg
     5941 cctaaagacg tggccgatga ggccaactgc caggatctca tccagaaggc ggtagacgag
     6001 ttcggccgga tagacatact cgtcaacaac gcagcccacc agatgacggt cgaagggata
     6061 gcagacgtat cgaccgacct gctcgaccgc accttcaaga ccaacatcta cgcgatgttc
     6121 tggctcgtca aggcagcgct tccacacatg cccgagggtg gttcgatcat caacgtatct
     6181 tccatccagg cataccagcc ttcaccaacg ctgcttccct actcgagcac gaaaggcgcc
     6241 atcatcacct tcacaaaggg gctcgcccag gaggtcgtac agtatgggct gcgggcgaat
     6301 acagtggcgc ctggtccggt gtggaccccg atcatcccgg cctccatgcc aggcgacaca
     6361 gtctccagct tcggcggcac ctcccccatg ggccgcccgg cccagccggt agagctcgcc
     6421 ccggccttcg tcttcctcgc ctcccaggaa gcgagctacg tgaacggcga gaccctcggt
     6481 gtcacaggag gccagccatt gccttgagag ccctcagcct ttcactcctc ctgtagccat
     6541 cggacaaaat gctattctgg tgcagtagcg tcggaaggta gcttgccctg ggaatcatcg
     6601 tccagaaata cggcgggagc tcggtggcga cggcggagca cataagggcc gtcgcagagc
     6661 gggtgaagag ggtccacgat tcgggtcacg gcctcgtcgt gaccgtctcg gcgatgggga
     6721 aacagacgga cagactgctg cgcctggcag gcgaggtaag ccgcgaccct tcgccgcggg
     6781 aaatcgacca gctactctct acgggcgagt tgcagtccgt cgcgctcctg gcgatggccc
     6841 tgcacgacag gggcgtcgcc gccacctctc tgacaggacc gcaggcaggc atgaagacca
     6901 cggggcgcta cggctcgggg cttatctcgg ggatacagcc ggaccgcctg cgaggcctgc
     6961 tcgacgaggg acaggtagcc atcgtcgcag gcttccaggg ggtcaatgcc ctcggtgata
     7021 tcatgacgct cgggcgcggt gggtcggaca ccacggccgt ggcgctcgcg gcggcgctcg
     7081 gggcggaacg ttgcgaggtc tttacggatg ttgacggcgt ctacaccgcc gacccgcgca
     7141 tcgtggcggg ggcgcggcgc atacccgtca tactctcctc ggagatggcc gagatgtcgt
     7201 ggcgcggcgc gaaagtgatg cacccgcggg ccgtggagct aggggcattg cacggggtcg
     7261 agatccacgt aaagagctcc ttcgaagagg gtcctggtac gatcatcact ggaggtaacg
     7321 gtttggaaca cctggagacc cgggagacgg tcgccggcat agtccacgac ctcgacgtct
     7381 cgcgcatcac cctgacaggc ataaagaccg gccctggtac gatgagccgc gtcttctccc
     7441 ctctggccga agcgggcatc tccgtcgacg tcatcgtgga gagcgccccg aaggaaggca
     7501 cctcggatgt ggccttcacc gtccgtcgcg gcgacttcag ccaggccctg cggatcgcgg
     7561 gcgcggtcgc ggactctttg aacgggcgta tcgagggcga ggagcagctt ggcaaggtct
     7621 ccgtcgtcgg gacggggatg ctcaaccgcc caggctacgc ggcgcggatg ttcgcgacgc
     7681 tcggggaggc cggtatcccc ataaggctgg tctccacctc agagatacag gtgacttgcg
     7741 tattgccggc aggaagggta gaagaagcgg taagggtttt gcacagggac ttcgagttgg
     7801 aggagagaga tgtttagcgg cgcgttcacg gccctcgtca cacctttcag gaacggggag
     7861 gtggacgtcg aagccttgga aggtatggtc gagttccaga tccacaatgg catccacggc
     7921 ctcgtcccct gcgggaccac gggcgagacc cctgccatga ccgaagagga ggaccgcctc
     7981 gtcataagca ccgtggtgag ggtcgcccaa gggcgggtgc ccgttatcgc gggctctggg
     8041 tccaacagta cggacatggc gatcaagtac acgaagatgg ctgaagagga gggtgctgac
     8101 ggctctctcc aggtcgcccc ctactacaac aagccgaccc aggaggggct gtacaggcac
     8161 ttcgccacga tagcggagag cacggatcta ccaatcatcc tctacaacat cccaggccgg
     8221 acggccgtaa ccatctcagc cgagaccata tccagccttt ccgagatccc gaacatcgtc
     8281 ggggtcaagg attcgaccct ctccatgaac atggttagcg acataaagag cctctgcggc
     8341 gaagagttcg ccatcctctc cggggatgat ccgatgacgc tccctcttat ggcccttgga
     8401 gggcacggtg tgatctccgt agcctccaat gtcgcccctg ctgtcgtctc cgatatggtg
     8461 aggaccctgc tcgaagggga ctgggagagg ggccgcgagc tccactacga gctgctgccg
     8521 ctcttccgcg cactcttcgt cgagaccaac cccattcccg tcaagacggc agcttcgatc
     8581 cttgggctgt gctcggacga gatgcggctg ccgatgatcc cactccaggg cgagaacctg
     8641 gatacccttc gtcgcgtgat ggaggagtcg aaccacctcc tcccgacccc ggaagaagtt
     8701 tgaccaaaat agcggtaata ggggcggcgg gccgcatggg acgcgagatc tgccgcgcct
     8761 ccctcgaaac ggaagggatc gaactggaag gcggcaccct cagagcaggg gccgcagagc
     8821 tcggctccga cctgggcgag ctctgcggcc tcggcaagac cagcgtaacc gccacagaag
     8881 acccgcccgg atcagccgag gtcctgatcg agttcaccac cccggaggcg accgtagagc
     8941 acctctctta ccagagaccg cacgtcatag ggacaacggg actctctgcc gagcaactgg
     9001 cgagggtcga ggaggcttcg gacagcgtgc ccatcgtccg cgcccccaac atgagcgtcg
     9061 gggttaacct gttgcgcgag gtggttcgcg aaatctcggc caagctcggc gagggctacg
     9121 acatcgaggt cgtagaagcc caccacagga ataagaagga cgcgccctcc ggcacagccc
     9181 tgtttctcgc ccgggccgcc gccgagggca gaaagaggaa cctggacgag gtcgtcgtct
     9241 acgggcggga gggtctggca cccaggcagg aaggtgagat cggcatccac gccctgcgcg
     9301 gcggcgcggt tgtcggggag cacaggctcg tcttctactc gggtggagag gagctcgagg
     9361 tcgtccaccg cgtcctctcc cgccgcacat tcgccgacgg cgctctcagg gccgcccgct
     9421 tcgccgcgaa cgcgaagccc ggcctgtact ctatggccga cgtgctcggg gacttctgag
     9481 gggaacacat gaccgacgct tccaacgtac ccctgatccc actcaaagtc ctcttcggta
     9541 acccggagaa ggtagcgccg cgtctctccc cagatggaga acgcctggcc tacctggccc
     9601 cgaaagacgg cgtcctcaac gtctgggtcg gtccggtggg gcgccccgcg ggcggcgttg
     9661 acttcgagcc ggtgaccgac gacagaaagc gcggcatcag ggtgttcttc tgggccgaga
     9721 acaataccca catcgtctac ctccaggacg agggcggcga cgagaactgg cgcgtgcacg
     9781 ccgtagatgc ctccacgaaa caggaccgtg acctgacccc cttcgacgac gtgcaggccc
     9841 agatcgtaga cagtagccgc cgtttcccgg atgcgctgct cgtcgcgctg aacagggaga
     9901 accaggagct ccacgacgtc taccgcctcg ccctctcgac gggcgacctg gaactcgtcg
     9961 ccaggaaccc cggcaacgcc gtccgctggg tggtagacgc acacctcagg gtgcgcggcg
    10021 cgatggcggc cacaccggac ggcggcttcg acttgctcgt gcgagaggga gccgacgaag
    10081 acgcggactg gcgcaagctc ctcggttggg gcaaggagga cgcgctctcc agcgggcccg
    10141 tcggctttac cgagagcggc gtgaagatgt acctcataga ctcccgcggg gccaacgcct
    10201 cccgactcgt cctgctcgac ctcgaaaatg gctcgacaaa gacgctagtc gaggacccgc
    10261 gctacgatgt cggcagggtc gtgaagcacc cagagaccca cgaggcccag gccgccgccg
    10321 tggagcgcgc gaggacggag tgggtcgtgc tcgacgacga catcagggaa gacttcgagg
    10381 tcataggcgg ccttggccgc ggagacttcg gcgtgactag ccgcgacagg gcggatgaga
    10441 actggctcgt cgccttcacc gccgaccaag gcggagcctc ctactacgcc tacgaccgca
    10501 agaacaaacg cggtgagcat ctcttcgacg cacgccccga cctcgcggag tacaccctcg
    10561 ccagaatgga gcccgtctcc ttcgcctccc gcgacggcct caccatagag ggctacctga
    10621 cgctcccgcc aggaacgtcc ggcggaccca tgccgatgat cttgaacgta cacggtggtc
    10681 cctgggtgcg cgacgggtgg ggctatgatc ccgaggcgca gtggttcgcc aacagggggt
    10741 acgcgtgtct gcaggtgaat tatcgtgggt cgacggggta cggcaaggag ttcttgaacg
    10801 cggggaacaa agagtggggc ggtactatgc acgacgacct cgttgacgcg gtcgggtggg
    10861 ccgtacagag gggcgtggcc gaccccgagc gggtcgcgat ctacggcggc tcctacggtg
    10921 ggtacgccgc actcgccggg gccacgttca cgcccgacct cttccggtgc gccgtcgata
    10981 tcgtgggtcc ttcgagcctg atcaccctga taaacagcat cccgccttac tggaagcctc
    11041 tcctcgcgac cttccacgag cgggtcggca atcccgacac cgaaggggac ttcctcgagt
    11101 cacgctcacc cctgttcttc gtcgaccgca tcaggatacc gatgctcatc gcccagggcg
    11161 cgaacgaccc acgcgtaaaa caggccgaat cggagcaaat agtcgccgcc atgaaggaga
    11221 aagacataga ttacgagtac ctgctcttcg aggacgaagg tcacggtttc gcccgtcccg
    11281 agaaccgtct gaagttctac gcagccgcag aacgctttct ggccaaacac ctcggaggac
    11341 gcgcacaaga gtagagggat acttcgggtg gggagcagtt cagtctgctg actccagagc
    11401 agtctcctcc tcccccgagg agaccacggt cgcgacccag ctcgcatcga cgcccaactt
    11461 gctcgctatc tccccatcag agaggccgag ccgccgaaaa cgttcgacct cctcagccag
    11521 acagacttcg ccgcggtctt ccaagctctc tcctcgcaac accattgcag gaattcgagg
    11581 ataccacgga cgtggatcag acggtcgagg gcaccttcag gctcatgccg gggtagatct
    11641 cataccccga gagatcgttg gcctcgcgga tggcctccac cttcggcctc ggatcctccc
    11701 agaccgcggt gtgctctatt gctattcccc agagcgtatc tccaggcgca acggtgtagt
    11761 ggacgggctg cggctcagcc cctgccacgc cacccacgta cagggccacg gcgagggccg
    11821 cggcaacgaa caaggctccg agcctgcgac ggcgatattt cgcgcggtct cttctcaccg
    11881 gggaaatata cgggaccact gttccgttcc caacggcatt ttctcgccgc caccgcgaac
    11941 aggccatccg gtagaatccg ctctatgcgt ttcaccaaga tgcaaggctg cggcaacgat
    12001 ttcctcatct tcgatcctgg cgaagtagag gggatggatc tccatgacct cgccacgaaa
    12061 gcctgcgaca ggcacttcgg cgtcggggcc gacgggatcc tcatccccgc tccctc
//
LOCUS       DASTKM010000355         4421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_339147, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000355 DASTKM010000000
VERSION     DASTKM010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339147"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VFQ10_14110"
     CDS             <1..1186
                     /locus_tag="VFQ10_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET9966523.1"
                     /translation="KPYPLPPLRPFRTSEIFSKATKEMGLHPYPTPVAVNSEPYNGYP
                     ATSYCAWSGGFGPFNDERWHPGLTSVPEALATGNLDLRTHCRVVSVLTDSSGHTSGVE
                     YVDANGTPQVQEARTVILCGYTFENVRLLLLSGDQRHPGGLGNNAGQVGKHFMTKMWA
                     DVYGYFPDVVFNKHTGPAAQMWSLDDFIAADFDSAAHGFVGGATPNIENQQLPIQISR
                     EGLPPDVPSWGKGYKDHLRHWQHVCAIRLQPESLSYETDFLDLDPRYRDRSGLGLPLV
                     RITCDLRENERRLADWMEREAENILYAMGAVRTWRGPRFRGVCSSHDLGGCRMGEDPA
                     SSVVDPDLRVHDTPGLYVFGGAVFPTCPGVNPTLTMWALCYRAAERLVERVRRGDEQW
                     LE"
     gene            complement(1384..2646)
                     /locus_tag="VFQ10_14115"
     CDS             complement(1384..2646)
                     /locus_tag="VFQ10_14115"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HET9966524.1"
                     /translation="MIQDRSPGRNWAGNYTYRAQKLHRPSTIEQLQEIFANAPCVRVL
                     GSRHSFNDIADSSELITLEAMPMDVVVDHEAETVSFNAALKYGELVEALNEEGVALHN
                     LASLPHISVAGAVATATHGSGVTNGNLATAVVGLELVTSDGQIIEISRGEPDFDGLVV
                     GLGALGAVTRITLDVEPAYEVRQRVFEGLSWEALFEHFDEIISCGYSVSIFTRWGETI
                     DQIWVKSRVTDEPEQVESDLFGAVAAAVDRHPILGLDATSCTPQLGRRGPWAERLPHF
                     RMGFTPSSGEELQSEYLLPRRYAVEAIEAVRSLADEIRPILQVSEIRTVAADRLWMSM
                     SYGQDTVGIHFTWKPEREAVEDMLVQLETALVPFKARPHWGKLFNADAAAIAPHYERM
                     SDFIRLVERLDPRGAFRNSWLKAHVLGE"
     gene            3315..4127
                     /locus_tag="VFQ10_14120"
     CDS             3315..4127
                     /locus_tag="VFQ10_14120"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET9966525.1"
                     /translation="MRPICFISDYGLTDVLEGTCHGVMKGIAPGATIIDVTHTVPGFD
                     VIQGAEALRHATQYMPEDSVYLTVVDPGDKTKRRALAAEVRSGAYLVGPDNGILLPAA
                     EALGGIVRAVQLTNPRYHVHPVSSAFHGRDVFSPVAAHLAAGANLDDLGEGVTPASMV
                     SLDFPGFRREPGGSVAAEIINIDRFGNARLSVMQEDLDLPYDTPLEIGIRDEVIEARY
                     VETFGTAEEGDLVAVPDSHWRLSLAVNRGNAARALLLSVGDEVRLKPSAETT"
     gene            complement(4176..>4421)
                     /locus_tag="VFQ10_14125"
     CDS             complement(4176..>4421)
                     /locus_tag="VFQ10_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966526.1"
                     /translation="PYSWRLEDMLIVNAGTASTLRLRGNTRPCYNVIEAEEGRVEVYR
                     KYPFKERELAVRFDRKTREYSRSEEKIGAEYSGHPQH"
BASE COUNT          856 a         1358 c         1310 g          897 t
ORIGIN      
        1 caaaccttac ccgctgccac cactgagacc cttccgcacg agcgagatct tctccaaagc
       61 caccaaggag atgggtttac atccctaccc gacgccggtc gccgtgaaca gcgagcccta
      121 caacgggtac ccggcgacct cctactgcgc ttggagtggg ggttttggtc ctttcaacga
      181 cgagcggtgg catcccggtc tgacctcggt ccccgaggcg cttgcgaccg gcaacctcga
      241 cctgcgaacc cactgcaggg tcgtcagcgt cctcaccgac tcgagcgggc acaccagcgg
      301 cgtcgagtac gtggacgcca atggaacccc tcaagtgcag gaggcgcgta cagtaatcct
      361 atgtggctac accttcgaga acgtaaggct cttattgctc tcgggggacc agcggcatcc
      421 aggtggcttg ggcaacaacg cgggccaggt cggcaagcac ttcatgacca agatgtgggc
      481 cgatgtctac ggctacttcc cggatgtggt cttcaacaag cacaccggtc cggcggcgca
      541 gatgtggagc ttggacgact tcattgccgc cgacttcgac tcggcggctc acgggttcgt
      601 cggcggggcc acccccaaca tcgaaaacca gcagttgccg atacagataa gccgcgaggg
      661 tttaccgccc gacgttccgt cctggggcaa ggggtacaag gatcacctta gacactggca
      721 gcacgtctgc gcgattcggc tccagcccga gtcgttgtcc tatgagaccg acttcctcga
      781 cctggaccct cgataccgtg accgtagcgg gctcgggctc ccccttgtac gcatcacttg
      841 cgatctgcga gagaacgaga ggcgccttgc cgactggatg gaaagggagg ccgaaaacat
      901 cctttacgcg atgggtgcgg tgagaacgtg gcgtggtccg cgctttcgtg gcgtttgcag
      961 tagccatgac ctcggcggat gccgcatggg cgaagatcct gcctcctcgg tggtggaccc
     1021 cgatctgcgt gtccacgaca caccaggact gtacgtcttc ggcggagctg tctttccaac
     1081 ctgtcccggg gtcaacccaa cactgacgat gtgggctctc tgttaccggg ccgccgagcg
     1141 tctcgtggag cgcgtccgac gtggtgacga acagtggtta gagtgagtgt gcagactgcc
     1201 gggcttgcgt cgctcggccg atcgcggacg aaatagattc agcgtgctgc cgcaactcat
     1261 gatgccttgc tagtgcaagg actgtgagcg tagcgaggac caagcgcatg gcggtcatca
     1321 tggaagctgt ccggctcgga gaggcagctt gtcacgctgg tccccctcct gaggggtgag
     1381 tcattactca ccaagcacat gagctttcag ccacgaattg cggaatgctc cgcgcgggtc
     1441 gagccgctcg actagacgga tgaagtctga catgcgctca tagtgcggag cgatcgccgc
     1501 cgcatccgca ttgaagagct tgccccagtg cgggcgtgcc ttgaatggga cgagcgcggt
     1561 ttcgagttgg actagcatat cctcaaccgc ctctcgctca ggcttccagg tgaagtggat
     1621 accgacggtg tcttgtccgt agctcatgct catccatagc cgatctgcgg ccactgtgcg
     1681 aatctcggac acctgcaaga tcggtcggat ctcgtctgcg aggcttcgca cggcttctat
     1741 ggcctccaca gcgtaccggc gcggcaatag atactccgac tgtaactcct cgccactgct
     1801 cggcgtaaaa cccatgcgga aatggggtaa gcgttcggcc caggggccgc gcctgcccaa
     1861 ctgcggcgta caggaggtcg cgtcgagtcc gaggatcggg tgccggtcaa ctgctgcagc
     1921 gactgctccg aagagatcac tctctacctg ctcgggctca tcagtgaccc ggctcttgac
     1981 ccagatctga tcgattgtct caccccagcg ggtaaagatg ctgacgctgt agccgcacga
     2041 gatgatctcg tcgaaatgct cgaagagagc ctcccagctt agaccttcga agactcgctg
     2101 ccgcacttcg taggctggct ccacgtcaag ggtgatccgg gtgaccgcgc ccagcgcgcc
     2161 aagcccgacc acgaggccgt cgaaatcagg ctcgcctctg gagatttcga tgatctgacc
     2221 gtcggacgtc acgagctcca ggcccacgac cgcggtcgcg agattgccgt tcgtcacgcc
     2281 cgagccgtga gtcgccgtcg ccacggcgcc tgcaacggag atgtggggaa gcgaagcaag
     2341 gttatgcaat gccacacctt cttcgttcaa ggcctctacc agctcgccgt actttagagc
     2401 cgcgttgaag gagaccgtct ctgcttcatg gtcgacaact acgtccatgg gcatggcctc
     2461 aagcgttatg agctctgagg agtcggcgat gtcgttgaac gagtgccgag aacccaagac
     2521 gcgcacacag ggtgcgttgg cgaaaatctc ttgcagctgc tcgatcgtcg atggccggtg
     2581 cagcttctgc gcccggtagg tgtaattgcc cgcccagttc cttcctggag aacgatcctg
     2641 aatcatagcc ttgctcttcc tcctgccctc ggtgtcacag tttgttcgct gataagaagc
     2701 cagctatgga taccaatctg cttcctctaa ggccacacag tagtcgagta atgtcactta
     2761 gcaaggatca ttaaagccgc taagaactac ctgcgcaggc tgctctccgc gttgctgcac
     2821 ggttgtagca caaaggctcc cgagcgtgtc aaaaaccttc tctggtcttc cacgtttcac
     2881 ctgcaaaacg aggtatcttg agagtgggcg atgccgggat cgaaccagcg tcctctgccg
     2941 tgtgaaggca gcgcgatagt ttgctagagg tttctcaaac gtgcagaatc cctgcaaata
     3001 gccgtatttc agcactaacg catttctcag cctttcagga gattcactcg ggttggtgcg
     3061 cggttgcgca cacgttgtgc tctcgatatc tcggccggta ggtatctacg atcctttgcc
     3121 taccgcctct attgaggact cgcacaaggc tgtggctgac cccgctcgac tctctgtata
     3181 gctgaacaaa gtcatgaaca ttggcatcat gacgttcatc accgacgaga ccctcgtcat
     3241 gggcaagaag caagccagtt gggacgaccg aatgctaggt gcccgtgtct gtgagggtga
     3301 cttatactgt tcttatgagg cccatatgct ttatatcgga ctacggcctc accgacgtcc
     3361 tcgagggaac ctgtcatggc gtgatgaagg gcatcgcgcc gggagctacc ataatagacg
     3421 tgacacacac cgtccccggg tttgacgtca tccagggcgc ggaggcgctg cggcacgcca
     3481 cccagtacat gcccgaagac agcgtctacc tgacggtcgt agacccaggg gacaaaacca
     3541 agaggcgggc gctcgctgcc gaggttcgca gcggcgccta cctggtcggc cctgacaatg
     3601 ggatcctctt gcccgcggcc gaagctctag gtggcatcgt gcgagcggtg caactaacga
     3661 acccccgata ccatgtgcat cccgtctcca gcgcctttca cggccgtgac gtgttctccc
     3721 cggtggcggc ccacctagcc gctggggcga atctcgacga tctgggagaa ggggtgaccc
     3781 cagcttcgat ggtttccctt gacttccccg ggttcaggcg ggaaccaggc gggagtgtcg
     3841 cggcggagat aatcaatatc gatcgtttcg gtaacgcccg gctctccgtc atgcaggaag
     3901 acttggacct cccctacgac acgcccttgg agatcgggat tcgggatgag gtcatcgagg
     3961 cgaggtacgt ggagaccttc ggaaccgcgg aggagggtga cctggtcgcg gtgcccgact
     4021 cccattggcg cttgagcctc gccgtgaaca ggggcaacgc cgcccgagct cttttgctat
     4081 ccgtcggcga tgaggttcgc ctcaagcctt cggcggagac aacctaacta tagatcttca
     4141 gcatccccgt tcgtaagagc ccactgaaat ctggctcaat gctgcgggtg tccgctatat
     4201 tcagcgccga tcttctcctc ggagcgggag tactccctgg tctttcggtc gaagcgcacc
     4261 gcgagctcgc gctccttgaa cgggtacttg cggtaaacct ccacccgccc ttcctctgcc
     4321 tcgatcacgt tgtagcaggg ccgggtgttg cccctgaggc ggagggtcga ggcggtcccc
     4381 gcgttcacga tcagcatgtc ctccaacctc caggagtacg g
//
LOCUS       DASTKM010000356         8602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1046521, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000356 DASTKM010000000
VERSION     DASTKM010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046521"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1103
                     /locus_tag="VFQ10_14130"
     CDS             144..1103
                     /locus_tag="VFQ10_14130"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9966527.1"
                     /translation="MARLRRNLVLALALGAAVYLILAVLSDLGELAAALESFDYALIP
                     AILGLVALSYVVRFFRWTYYLRVLEVSVPTLINAAIFTSGLSMTISPGKLGEVLKSVF
                     VRRATGAPIARTAPAVFAERATDGTGMVLWGFLGAFALDLPPWTMLIFLALAAFGIAV
                     LRSKRLSLAAESVLSKLPLVNRLAPHLSDFHGASNELLGTRPLLVGTAISFCSWGLEC
                     AGVYLCAVGLGVEGPFLLVVFVFALSSLLGVLSMLPGGIGAVEAGLYGQFVAVANLST
                     GLAGALTLVIRLATLWFATLLGICGLLLVRLMLGDAPLEPHEK"
     gene            complement(1117..1812)
                     /locus_tag="VFQ10_14135"
     CDS             complement(1117..1812)
                     /locus_tag="VFQ10_14135"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HET9966528.1"
                     /translation="MKRGFTGLGRVGLFTFGVIALLAIGALALAGRGMLTGADAQPAK
                     RAIQPTGTEEATPEPVAKADLKARFVEEPSPVGDEASASASVPAGAMMSLSVPAMGIS
                     NIPVVEGTSETSLSQGAGHLPGTGYPWVPGSNTYIAGHRIGYPGTPSDHVFWNLPNLG
                     LGDEILLTDSNGTTYTYAVSEIFEVSPTDLSVTTPTGGDVVSLQTCIEDYGDYWTPGP
                     NWFVRYVVRAERV"
     gene            complement(1986..3197)
                     /locus_tag="VFQ10_14140"
     CDS             complement(1986..3197)
                     /locus_tag="VFQ10_14140"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9966529.1"
                     /translation="MKASAFRRLWAGAGLSMLADQAFLVALTWLVLRVTGSGAELGLV
                     LAVASIPGTILTPAGGVLSDRYSPALIMIWTSAGRVLLLALLTFLIMADATRIWHVYV
                     IAAGLSALDALYYPASMSIVPTLVDRARLNAANALSQAAEQVSSIFGPALAGTLLALL
                     GLGASFGATAALFLIATALFGAVARAAKSSGPQASDVPEEDKGQSGARIEFSEGISYV
                     WRDGVIRSLLLILLCTNLAMMGPLYVSGAVLAESRLGGAGAFASLVGAAGAGALIGVV
                     GAGSVSRFRRRGLIELGLTGLLGIIVGAIAFVPNLVAAVALATSIGAISSFLGVITIS
                     WLQERAEPRLIGRVMSFAMFSAVALDPISFALAGVLVELDLTAMFLAAGTLLLLTAVL
                     GALSHTMREVD"
     gene            3373..4029
                     /locus_tag="VFQ10_14145"
     CDS             3373..4029
                     /locus_tag="VFQ10_14145"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9966530.1"
                     /translation="MHIAQKFEGLLEMYRRPDGRKWSGQEIDEATGGIVTRSYITNLR
                     KGRIENPGFEKLKAIAKVMDFPPELWFEESPALRDGAPIDPVPGHLSVAEKTNRLFDI
                     IKNETTGRPYTNAEVARLSLGDLTEEQVQQIRAGKLTDPSMKQVVTLAEVFGVHPTYF
                     LDSTRKLPLLDQEAMSIFRDETVSAIAHKSLHLPGREKQMILGIIRQFEGAHQVDDER
                     "
     gene            4139..4669
                     /locus_tag="VFQ10_14150"
     CDS             4139..4669
                     /locus_tag="VFQ10_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966531.1"
                     /translation="MLTAAEFQEELAVLGLSGRRFEPEDYSDALGEHLNISITVHVIP
                     DSWYPELSRLLAISGRLGELRYSEQLNLAAILVPGSLPPLVLTLTILHELGHLAAGDL
                     LIEPDEQFEEDDQHAEQTMFETLPLKKGKKLARGYPFAKESLREREANLRASYALFAG
                     CLGDDSPYAHGMYDVL"
     gene            4666..6018
                     /locus_tag="VFQ10_14155"
     CDS             4666..6018
                     /locus_tag="VFQ10_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966532.1"
                     /translation="MSAAQHQALVLGSVSFVAFIFFLCFLLPSKRTKFSVFAACNLFF
                     VALMFGAVALVDLSGGRAEIMVAVMTELPVVLIVAWVCLALAGGLPAQLPAEPVARRP
                     WLRAILGSAPLVLLLSVGLATVVGLVWPSPALRVYAPAPPQFFVLKGLIMTPEVIYSG
                     FAAMVFVMAGRATGLKRRLYFKNLAFSVGMVCIALIALESTVFAGLRVWLSREGRRAI
                     LETLNTLEACLAICCILAFTAGLSLRYTPAVAMTLLHRLQTGWLRTQEQFESLEWQAV
                     SSGAAGRLTHASDAVVEACRIEGLSESDTQRALATIRLVAVMRDPSSETQQITPDAAR
                     KLYELQEEILCDDVLASKICWAAGWRSRALEPQTVKSAPLHDALKAALELVDNHGESE
                     DVRTPPLWYYIVAVSATDAKMIDSTHLSMQLRDGTERRAAVEAYDAAKRVLRMLEDHD
                     "
     gene            6115..6339
                     /locus_tag="VFQ10_14160"
     CDS             6115..6339
                     /locus_tag="VFQ10_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966533.1"
                     /translation="MQHENHVDDRLAGGSFGISGLLARARHRSEQEQGYPASESTSPY
                     VSAALGQTPYVAVGNGAIVVQRRGCGNWNC"
     gene            6416..7354
                     /locus_tag="VFQ10_14165"
     CDS             6416..7354
                     /locus_tag="VFQ10_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966534.1"
                     /translation="MDPAASEMLSARRVGEEAERAALSLPLKQEKVFESYYTYLTVDE
                     PAVHLMPLHFLPHASRAQVGEGSLRELAVAGKLEYARNRWLDEIVDHPGSAPALSPAH
                     RLNDALIALINARYASVLDGAAGASFFPVLSVLHARHGLSLILDGARFEGVIPRITLE
                     EYTEHATTRHGPVRAPVDAVLLLTGAADDLLQRARSCWHNWALGVQFYDDALDVEEDF
                     RNRNLTWVVGRALEHFHRAADNPTTRRMPDPDTFYERALAEGVVSEALSHAESFFAES
                     ARLAEPAFPSWVPFQKACVSQVRRLREDYEKLVTGA"
     gene            7385..7957
                     /locus_tag="VFQ10_14170"
     CDS             7385..7957
                     /locus_tag="VFQ10_14170"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HET9966535.1"
                     /translation="MDRLLVGVSGSVAVLNLPSYLATLRAELAREVRVIMTAAAASML
                     PPSTVALVCDGVFLDQEPTVEKRPGHIELARWCEMFVVLPATANVIGQTANGLGSNLL
                     TTTILASPKPVVFYPNVNDVMWSKAAVQRNVDTLRGDGHIVIEPEVALAYEVDSGETR
                     ESLVVPEPTQLVEQLREIHHQPEFLPVSGS"
     gene            8245..>8602
                     /locus_tag="VFQ10_14175"
     CDS             8245..>8602
                     /locus_tag="VFQ10_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966536.1"
                     /translation="MEGRAKLGRLTAPLALALMVAALWAGGPATGQAGGDPVLVGAGD
                     IASCRSTGDEATAGLLAGIDSTVATLGDNAYPKGTDANFAECYEPSWGQFKARTMPSP
                     GNHEYETPGASGYFDYF"
BASE COUNT         1690 a         2661 c         2576 g         1675 t
ORIGIN      
        1 tacggggtct tccgctacat gttgctcgtc caccgcgacg gcggcggcaa ccccgacacg
       61 ttgctcctct ccgacaggcc gctccagatc tcacttctcc tctggctggt cgtggtcggg
      121 acggtcatct acgtaatacc cttgtggcac ggctgaggcg aaatctcgtc ctcgccctcg
      181 ccctgggcgc cgctgtctac cttatcctcg ccgtcctctc cgacctcggc gagctcgccg
      241 cagccctcga gagcttcgac tacgccctca taccagcgat ccttggcctc gtcgccctct
      301 cctacgtcgt acgcttcttc cgctggacgt actacctcag ggtacttgaa gtctccgttc
      361 caacgctgat aaacgccgcg atcttcacgt caggtctctc gatgacgatc tcaccgggca
      421 agctcggtga ggtgctgaag agtgtcttcg tccgcagggc cacgggagcc ccgatagcgc
      481 gcacggcacc cgcggtcttt gccgaacgcg ccaccgacgg tacaggtatg gtcttatggg
      541 gtttccttgg cgcctttgcc cttgatctac ccccctggac aatgctgata ttcctcgccc
      601 tagcggcctt cggaatcgcc gtcctgcgct ctaaacgcct ctctctggcg gccgaaagcg
      661 tcctctcgaa gctgccgctc gtcaaccgcc tcgcccccca cctgagtgac ttccacgggg
      721 catcgaacga gctcctgggg acgagaccgc tcctggtggg gaccgccatc tccttctgct
      781 cgtggggcct ggagtgtgcc ggcgtgtatc tttgcgccgt ggggcttggc gtggaggggc
      841 ctttcctcct cgtcgtcttc gtgttcgcgc tgagttcgct cctcggggtc ctgagcatgc
      901 ttcccggtgg gataggggcc gtcgaggccg gtctctatgg gcagttcgtc gccgttgcga
      961 acctctcgac aggcctcgcc ggcgcgctta cgctcgtgat ccgcctggca accctctggt
     1021 tcgccacact actcgggatc tgcggactac tgctcgttcg gcttatgctg ggtgacgccc
     1081 cgctggagcc acacgagaag tagcccccgg atccccctac accctttcgg cgcggacgac
     1141 gtaccggacg aaccagttgg gaccaggggt ccagtagtcc ccatagtctt cgatgcacgt
     1201 ctgcaaagag accacgtcgc cgcctgtggg cgtggtcacg gatagatctg tgggcgacac
     1261 ctcgaagatc tcgcttacgg cgtaggtgta ggtcgtaccg ttcgagtcgg tgagtaagat
     1321 ctcgtccccc aggcccagat tgggcaggtt ccagaacacg tgatcgctcg gggtgccagg
     1381 gtatcctatc ctgtgaccag ctatgtaagt gttggagcca ggaacccaag ggtagccagt
     1441 gccaggaagg tgcccggccc cctgcgagag gctcgtctcc gatgtgccct cgaccacggg
     1501 aatgttggag atacccatag cgggaacact caaagacatc atcgcgcctg cagggacgct
     1561 agccgaagca gacgcttcgt cccccacggg agagggttcc tcaacaaacc gcgctttcag
     1621 atccgccttg gcgacaggct ccggtgtggc ctcctccgtc ccagtaggct ggatggccct
     1681 cttggcaggc tgggcgtcag cccccgtgag catcccccta ccggccagag cgagcgcgcc
     1741 gatagcaagc agcgcaatga cgccaaacgt aaagagaccg accctcccta ggccggtgaa
     1801 tcccctcttc acacgtcccc tacttctccc caagacaagg tctttctatg gtccctcccc
     1861 aacaagcccc agtacatcag ctatgactcg ccggacttta acatcacaac acacgaaccg
     1921 caagaaactt aacaagtttg tagcatattg ttccaagagc gatcggtaac gatcgacaag
     1981 ctgcgctagt ctacttctcg catcgtgtgg ctcagggctc ccaatacggc cgttagcagc
     2041 agaagtgtgc ctgcggccag gaacatagct gttagatcga gttcgacgag taccccggcg
     2101 agggcgaagg agatggggtc aagggcgacg gcggagaaca tcgcgaagct catcacccgt
     2161 ccgatcaaac gcggctccgc gcgttcttga agccaggaga tcgttattac gccgaggaag
     2221 ctcgatattg cgccgatcga ggttgccagg gcgactgcgg ccaccaggtt gggtacgaag
     2281 gcgatcgctc cgacgatgat accgagcagg cctgtcaatc ccagctctat cagaccccgg
     2341 cgtctgaaac gactcacaga tcccgcgccg acgaccccga tcagagcccc ggctcccgcg
     2401 gcccccacca gtgaggcgaa ggcgcctgcg cctcctaggc gtgactcggc gaggacggct
     2461 ccactgacgt acagaggtcc catcatggcc aggttggtac agagcagtat cagtaacagc
     2521 gaacgaatga ccccatcgcg ccacacgtag cttatccctt cgctgaactc gatccgtgcg
     2581 ccgctttgcc ctttgtcttc ctctggtaca tcactggcct ggggccccga cgactttgca
     2641 gcccgtgcga cagcgccgaa caatgctgtg gcgatcaaga acagagcagc agtagcgcca
     2701 aagctggctc caagaccgag gagcgcgagc agagtacccg ccaacgctgg cccgaagatg
     2761 ctgctgacct gctcggcagc ctggctcagg gcgttcgccg cattcaaacg ggcgcgatcg
     2821 accagggtcg gtacgataga catcgacgca gggtagtaga gtgcgtcgag cgcgctcaag
     2881 cctgccgcaa tgacgtacac atgccagatt cgggtcgcat ctgccatgat caagaaggtc
     2941 aaaagggcta ggagcaaaac gcgtcccgca ctggtccaga tcatgatcaa cgcgggcgaa
     3001 taccgatcgc tcaagacccc gcccgccggt gtcaggatgg tgcctggtat cgatgcaaca
     3061 gccagcacca gaccaagctc cgcaccagag ccagtaacgc gtagcaccag ccacgtcaga
     3121 gccacgagga aggcctggtc cgcgagcatc gagagccccg ccccggccca cagccgtcgg
     3181 aaagcgcttg ccttcaaggg catccagaag cgaagtgcta gtaatcgccc cagcacacct
     3241 acccctcggg cccggcggac gctattctct cgttcaacga accttctccc gtagttgtat
     3301 tgcttaaacg atatagagta tggtatcgta tattgaactg caagactaat ggggtggagg
     3361 cggagggctc agatgcatat cgcgcagaag ttcgagggcc tcttggagat gtaccgtcgt
     3421 ccggacgggc gcaagtggag tggccaggag atagatgagg ccacgggtgg tatcgtgacg
     3481 cgctcttaca tcaccaactt gcggaagggc aggatcgaga accctggttt cgagaagttg
     3541 aaagccatag ccaaagttat ggacttcccc ccggagttgt ggttcgagga gagtccggct
     3601 ctcagggacg gggcgcctat cgaccccgtg cctggacacc tgagcgtcgc cgagaagacc
     3661 aaccggcttt tcgatatcat aaagaacgag acgacgggta gaccttacac caacgccgaa
     3721 gtggcgcgcc tgagcctggg agacctgacc gaggaacaag tccagcagat cagggcggga
     3781 aaactgaccg atccgtccat gaaacaggtg gttaccctgg cggaagtatt cggcgtgcac
     3841 cccacgtatt tcctcgattc gacgagaaag ctgccacttc tggatcaaga agcgatgagc
     3901 atctttcggg acgagactgt aagcgccata gcccacaaga gcctgcactt gccagggcga
     3961 gagaaacaga tgatcctcgg tatcatcagg cagttcgagg gcgcacacca ggtcgacgat
     4021 gaacgttgac tcgccgaaac gtgacctcgc aggccctttg ctggacaggt tgaccggctt
     4081 ctggcgccct cttcgaaacc tcggcgccgc acgctcacgt gtcaacggat cgagatcctt
     4141 gctcacggcc gcagagtttc aggaagagtt ggctgtcctg ggcctgtcag gccgtaggtt
     4201 cgaaccagaa gattattcgg atgctctcgg agagcacctg aacatctcca taaccgtaca
     4261 tgtgataccg gactcctggt accccgagct ctcgcgcctc ctggcgatct cgggacggct
     4321 tggggaactt cgatactcgg agcagttgaa cctggcagcc atcctcgtac caggcagcct
     4381 gcccccgttg gttctgacct tgactatcct gcacgagctt gggcacctgg ccgctggcga
     4441 cctattgatc gagcccgacg agcagttcga ggaggacgat cagcatgccg agcagaccat
     4501 gtttgagacc cttcccctga agaaagggaa gaagctagcg cgaggatacc catttgccaa
     4561 agagtcactc cgggagcgcg aggccaattt gagggcttcg tatgctctct tcgccggctg
     4621 cctgggtgac gacagtccct acgcccacgg tatgtacgac gtgctatgag tgcggcccaa
     4681 caccaggctc tcgtgcttgg cagtgtaagc ttcgtggcgt tcatcttctt cttgtgtttt
     4741 ctccttccat cgaaacgtac gaagttcagc gtcttcgccg cgtgcaacct gttctttgtg
     4801 gcgctgatgt tcggcgccgt agccctggtc gacctctccg gcgggcgtgc ggagatcatg
     4861 gtggcagtta tgacggagtt gccggtggtg ctcatagtag cgtgggtgtg cctcgccctg
     4921 gctggtggcc tgcccgccca actcccggcg gagccggtgg cgcgaaggcc ctggttgcgg
     4981 gcgatactgg ggtcggcacc actcgtccta ctactttcgg tgggtcttgc tactgtcgta
     5041 ggactcgtct ggccatctcc ggccttacgt gtttatgctc ctgcgccgcc ccagtttttc
     5101 gtattgaagg gtttgatcat gacccccgag gtaatctact cgggctttgc cgcaatggtc
     5161 ttcgtgatgg ccgggcgggc aacagggctc aaacgacgcc tctacttcaa gaacctcgcc
     5221 ttctccgtcg gtatggtgtg catagccttg atagcgttgg aatcgacggt ctttgccggc
     5281 ttgagggtct ggctctccag agaaggccgg cgagctatcc tggagacgct gaatacccta
     5341 gaggcatgcc tggcgatctg ttgcatcctc gcttttacag ccggactatc cctacggtat
     5401 acgcctgccg ttgccatgac gctcctgcac aggcttcaga caggatggct gcgtacccag
     5461 gaacagttcg agtccctcga atggcaggcc gtcagttctg gtgcagcagg ccggttgacg
     5521 cacgcatccg acgccgtcgt agaggcgtgc cgcatagagg gtctctcaga atcggacacg
     5581 cagagagccc tggctacgat ccgacttgta gccgtcatga gagatccttc gagcgagaca
     5641 cagcaaatta cgcccgatgc ggcgcgcaag ctctacgaac tccaggaaga gatcctgtgc
     5701 gacgatgtcc tggcatcgaa gatatgctgg gctgcaggct ggagatcacg cgccctcgaa
     5761 cctcaaacgg tcaagtctgc gccactgcac gacgcgctaa aggcagcact cgaacttgtc
     5821 gataatcatg gtgagagcga ggacgtacgt acgcccccac tctggtacta catagtcgcc
     5881 gttagcgcga ccgatgcgaa gatgatcgat tcgacccatt tatcgatgca gcttcgcgac
     5941 gggaccgagc gccgagcggc cgtcgaggcg tacgatgccg cgaagcgcgt gttgcggatg
     6001 ttggaagacc acgactgaga atggagggtg cgagaaggat agattgcacg caatggagta
     6061 ggtaatctcg taagagagga cagggaagac cgagtaagtg agggagggat caggttgcaa
     6121 cacgaaaacc acgtcgacga ccggctggct ggcgggtcgt tcggcatatc cggattgttg
     6181 gcaagggcca ggcaccgcag cgaacaggaa caaggctacc ccgcctcaga gagcacttca
     6241 ccctacgtta gtgccgctct cgggcaaacg ccttacgtgg cggtgggcaa cggcgccatc
     6301 gtcgtgcaac ggcgaggttg cggcaactgg aactgctgaa gagacgttcc aggactgagt
     6361 gccgcaggtt tttgggggcg acgtgtgcgc atcacgggca gaacgctcgg agggtgtgga
     6421 tcccgccgca tccgagatgc tgagcgctcg ccgggtcggt gaagaggctg agagggcggc
     6481 tctctcgctg cctctgaaac aggagaaggt cttcgagtct tactacacgt acctgaccgt
     6541 ggatgaaccc gctgtacatc tgatgcctct tcactttcta ccccacgcat cgcgggcaca
     6601 ggtcggcgag ggctcgctgc gcgagctggc cgtggcgggg aagttggagt acgcgcgcaa
     6661 ccgctggctc gacgagatag tcgaccatcc tggttctgcg cccgccttat ccccggcaca
     6721 ccggctcaac gacgccctga tcgcgcttat caacgcgcgc tacgccagcg tcctcgatgg
     6781 tgctgccggt gcgtccttct tcccagtgct ctccgtgcta cacgcccgcc atgggttgtc
     6841 gctgatcctc gacggagccc ggttcgaggg ggtcatcccc cggataaccc tggaagaata
     6901 caccgagcat gccacaacga gacacggtcc cgttcgcgct ccagtggacg ccgtgctcct
     6961 actcacgggg gcagcggacg accttcttca aagggcgagg tcctgctggc acaactgggc
     7021 gcttggcgtc cagttctacg acgacgccct cgatgtagaa gaggatttcc ggaaccgcaa
     7081 cctgacttgg gtcgtggggc gcgctctcga acactttcat cgggccgccg ataaccccac
     7141 cacacggagg atgccggatc cggacacgtt ctacgagcgg gctcttgccg agggtgtggt
     7201 ctccgaggct ctaagccacg cggagtcatt cttcgccgaa tccgccaggc ttgccgagcc
     7261 ggccttccca agctgggtac ctttccagaa ggcatgcgtg agccaggtca ggcgccttcg
     7321 agaggactat gagaagctgg tcacaggggc ctgagatact ggaatagagc agggagggga
     7381 accgatggat cgtttgttgg taggggtgtc agggtctgtg gctgttctca accttccttc
     7441 ctaccttgcc accctgcgcg ccgagctcgc aagagaggta agggtgatca tgaccgccgc
     7501 cgcggcctcc atgctgccac cctctaccgt cgccctggtc tgcgatggag tctttttgga
     7561 tcaagaaccc actgtagaga agagaccagg gcacatagag ttggcccggt ggtgcgagat
     7621 gttcgtagtc ctacccgcta cggcgaacgt aatcgggcag actgccaacg gcctcggttc
     7681 gaacctactg accaccacca tcctcgcttc accgaaacca gtagtcttct atcccaacgt
     7741 caacgacgtt atgtggagca aggccgccgt acaacggaac gtcgacacct tgaggggaga
     7801 tggccacata gtgatcgaac cggaagtcgc tctcgcttac gaggtcgact cgggggagac
     7861 gcgcgagagc ctggtagtcc cagagccaac gcaactggtc gaacagcttc gagagatcca
     7921 ccatcagccc gagtttctac ccgtctccgg ttcttagctt ccggtttcgt agagtgatca
     7981 ctctccttcg gcctttctcc tccaactttt attcacccaa cctcgtatgg ggcaggagct
     8041 ttctcggtgg ttgcacaaac cttcacaagc cttcatactt atcctcgttg tacacacgtg
     8101 gcggggcagc atgccccggc ctctccaaaa gggtggggtc gatgaatcgc ttcctgatgt
     8161 ttgtgagggt actgaccccg tccgcaatgg ccgtggcgat cgccgaacag catgtctcgg
     8221 tcttgcgagg tgcaccttag agcgatggag ggtagggcga agctcgggag gctaaccgca
     8281 ccgctagcgc tggcgctgat ggtagcggcg ctttgggctg gcgggccggc cacggggcag
     8341 gcgggcggcg atccggtcct ggtcggggca ggggacatag cctcctgcag aagcaccgga
     8401 gacgaggcga ccgccggcct cctagcgggc atcgatagca cggtcgctac cttgggcgac
     8461 aacgcctacc ccaagggtac agacgccaac ttcgccgagt gttacgagcc ctcctggggc
     8521 cagttcaagg cgcgcaccat gccaagccca ggcaaccacg agtacgagac gcctggcgcc
     8581 tcaggctact tcgactactt cg
//
LOCUS       DASTKM010000357         5348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_514054, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000357 DASTKM010000000
VERSION     DASTKM010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514054"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFQ10_14180"
     CDS             complement(<1..547)
                     /locus_tag="VFQ10_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9966537.1"
                     /translation="MRQTLRPDLVVDAHGASSDVGISVEHGRLAAVGPATDGERLPGR
                     ALAPGFVNDHSHAFQRGLRGAVEQIEPSQPWDDFWTWRERMYSLAGGLDPDSIREVSS
                     RCYGEMLSAGYTSVTEFHYVHHRPEGTPYEDPNTLAKAVALAAEDTGIRLLLLPVAYA
                     RDGLPRFRDQSVRAFLQRVDDL"
     gene            complement(544..2211)
                     /gene="hutU"
                     /locus_tag="VFQ10_14185"
     CDS             complement(544..2211)
                     /gene="hutU"
                     /locus_tag="VFQ10_14185"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET9966538.1"
                     /translation="MPDATEVPTIRAPRGRDLTCKGWHQEAALRMLMNNLDPQVAERP
                     EDLVVYGGTGKAARNWESFWAIVSTLQGLDDDETLLVQSGKPVAVFRTHPWAPRVLIA
                     NSLLVPEWADWKTFRELEKAGLTMYGQMTAGSWIYIGTQGILQGTYETFAAVAQKRFG
                     GTLRGRVCLTAGLGGMGGAQPLAITMNEGVALCVEVEGHRIDRRISHGYLDERIDDLD
                     GAVERAAEAKRAGEPRSIGILGNAAEVFPGLLERGYVPDVVTDQTSAHDPLAGYIPAG
                     YDLEAASELREADPERYISEARASMASHCAAMVGFQERGADVFDYGNSLRGEAQLGGF
                     EGAYGYPGFVPAYIRPLFCEGKGPFRWAALSGEESDIAATDDAILELFPDDERLARWI
                     SEARQKVHFQGLPARICWLGAGERHRAGLRFNELVANGTISAPIVIGRDHLDTGSVAS
                     PYRETEAMKDGSDAIADWPILNAMLSVAGGASWVAVHHGGGVGIGRSIHAGAQVLADG
                     TEDGAARLQRVLFNDPGIGIVRHADAGYESAQAAATRLGIQMPALES"
     gene            2271..2444
                     /locus_tag="VFQ10_14190"
     CDS             2271..2444
                     /locus_tag="VFQ10_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966539.1"
                     /translation="MGSSMVATMPIGAVLGSLFSNPSSLGFVFVPMMGLIAISGILST
                     TPFPRIGSGERSA"
     gene            complement(2549..3514)
                     /locus_tag="VFQ10_14195"
     CDS             complement(2549..3514)
                     /locus_tag="VFQ10_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9966540.1"
                     /translation="MKNRTKLIRAIPALLAALALLAFAGCGNVGSSSGSGPSAGDDSG
                     GGGPTITVGSKNFTEQYVLGELYAQALAANGFSVETKLDLGSSAIADKALQNGQIDMY
                     PEYTGTALVEIFKQMEQPDTPDATYQEAKKAYADRDPANTMLEPAPFNNTYGIFVRRE
                     VAEEYNLQTLADLAEASGDITFVCFSEFLKRSDGVPNMKKNYEDLEFGDVKVVNSIGG
                     PIYQGVLQGEGDAGVGFTTDSQLASDELVVMEDQKNIWPFYYPAPVVRSDVLEENPEM
                     EEVLNSVSESLDADIMRQLNAQVDIEQEDPEDVAKEYLEEQGIVK"
     gene            complement(3537..4187)
                     /locus_tag="VFQ10_14200"
     CDS             complement(3537..4187)
                     /locus_tag="VFQ10_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9966541.1"
                     /translation="MSGILDILRRPDFFELLRQHILLSSAALLIGIAIALPVALAIRN
                     TSLGAALAVNIGNIGRAVPSLAILALCQPFLGFGFDTALVALTALAIPPILINASTGL
                     REVSKDVIDAARGMGLSGSQILSSIQLPIAAPLIFAGIRTSAVQVVASATLATFIGGG
                     GLGELIVEGTQLNDTTILLAGSFSVAALAIITEVGFGVLERAMTPKGLKIAQKRGR"
     gene            complement(4189..4893)
                     /locus_tag="VFQ10_14205"
     CDS             complement(4189..4893)
                     /locus_tag="VFQ10_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9966542.1"
                     /translation="MANPALALLQDLSSIEDPKLIEWDWISDNFWEDIVPAIQGHIFL
                     SFVSVAIALMISLPTGVLVSRYRKAYPPVTFVTGLLYSIPSLALFGILISIPGITIGP
                     TPVIIALVAYSLLILIRNTVAGLDAVPAETIDAARGMGLTDGQILFRVELPLALPVIV
                     AGIRIATVTIIGIATIGAYIAGGGLGKMIFDGINRSFPTMIITGAILATVLAIIADLA
                     LLALERYLRPWARRRT"
     gene            complement(4886..>5348)
                     /locus_tag="VFQ10_14210"
     CDS             complement(4886..>5348)
                     /locus_tag="VFQ10_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966543.1"
                     /translation="VDPITRERLQDEFLRIQEDIKKTIVFVTHDIDEAIKMGNKIAIL
                     KQGGVLAQYDIPETILANPNSEFVASFVGTDRILKRLSLVRVGDMRLEPANGEAEDLP
                     RIDGSLTVRDALSEIIGSGSTKGVVERDGEKGLLTIDAIEKLSSARDDSRG"
BASE COUNT          895 a         1630 c         1767 g         1056 t
ORIGIN      
        1 tgagatcatc caccctctga aggaacgctc tcacggactg gtcccgaaag cgcgggaggc
       61 cgtcccgcgc gtaggcgaca ggtagcagga ggagccggat acccgtatct tcagctgcca
      121 gggctacggc tttcgccagg gtgttggggt cttcgtaagg cgttccttcg ggacggtggt
      181 ggacatagtg gaactcggtg acgctcgtgt atccggcgga gagcatctcc ccgtagcaac
      241 ggctgctgac ctcgcgtatg gagtcggggt ccagcccccc ggccagggag tacatccgct
      301 ctcgccaggt ccagaagtca tcccagggtt gggaaggttc gatctgttcg acagcacccc
      361 gcaaccctcg ttgaaaggcg tgactgtgat cgttgacaaa accgggggcg agcgcccttc
      421 ctggtagccg ctccccgtct gtagcgggac cgaccgcggc tagccttcca tgttctacag
      481 agatgccgac gtcactactg gccccatgag catcgacaac gaggtcgggg cgtagagttt
      541 gcctcaactt tctagtgctg gcatctggat gccaaggcgc gtggccgccg cctgcgccga
      601 ttcgtaaccc gcgtcggcgt ggcggacgat gcctatgccc gggtcgttga agagcacgcg
      661 ctgcaggcgg gcggcgccat cttccgtgcc gtcagcgagg acctgggcgc cagcgtggat
      721 ggagcgccct atccccacgc ctccgccgtg gtggaccgcg acccagctcg ccccgccggc
      781 gacgctcagc atggcgttca ggatgggcca gtctgcgatg gcgtcggagc cgtccttcat
      841 cgcctcggtc tcgcggtagg gtgaggcgac gctgcccgtg tccaggtggt cccgccctat
      901 gactatgggg gcggagatgg tgccattggc aaccaactcg ttgaagcgga ggcctgcccg
      961 gtgccgttcg cctgccccga gccagcagat gcgcgcgggg aggccctgga aatggacctt
     1021 ctgtctggcc tcggagatcc aacgcgccag acgctcgtcg tcgggaaaga gctccaggat
     1081 cgcatcgtcc gtcgccgcta tatcggactc ctcgccggag agcgcggccc accggaatgg
     1141 tcccttgccc tcgcagaaga ggggccttat gtacgctggc acgaacccgg ggtagccgta
     1201 agcaccttcg aagccgccga gctgagcctc cccgcgcagg gagttgccat agtcgaagac
     1261 gtcggcgccc cgctcctgga aacccaccat cgccgcgcag tggctggcca tcgaggcgcg
     1321 ggcttccgaa atgtagcgct ctgggtcggc ctcgcgcagt tcggatgcgg cttcgagatc
     1381 gtagcctgcg gggatgtagc ccgcgagcgg gtcgtgggct gaggtctggt ccgtaacgac
     1441 atcggggacg tagccgcgct ccaagagccc aggaaacacc tcggctgcgt tgccgaggat
     1501 tcctatggac cgtggttctc cggcgcgctt cgcttctgct gcgcgctcta ctgctccgtc
     1561 taggtcgtct atccgctcgt ccaagtagcc gtgggagatg cgccggtcta tcctgtgccc
     1621 ctctacctcg acgcagaggg ccacgccctc gttcatggtt attgcgaggg gttgggcgcc
     1681 gcccataccg ccgaggcctg cggttaggca gacacggccg cgcagcgttc ccccaaagcg
     1741 tttttgggcg acggcggcga acgtctcgta ggtaccctgg agtatgccct gggtgccgat
     1801 gtagatccat gaccccgcgg tcatctggcc gtacatggtg agtccggcct tctcgagttc
     1861 cctgaacgtc ttccagtcgg cccattccgg gacaaggagg gagttggcga tcagaacccg
     1921 cggagcccag gggtgcgtcc tgaagacggc taccggtttt cctgactgga ccagcaacgt
     1981 ctcgtcgtcg tccagtcctt gtagggtact gactatggcc cagaagctct cccagttcct
     2041 ggcggccttg cccgtcccgc cgtagacgac gaggtcttcg ggacgttctg ctacttgggg
     2101 atcgaggttg ttcatgagca tgcgaagggc tgcctcctgg tgccagccct tgcaggtgag
     2161 atccctccct cgcggcgcgc ggatggtagg tacttctgtg gcgtccggca tgggatctcc
     2221 ttccgtgaaa tcaggattca gtcttgcacc ggctactcga aaagtccagc atggggagta
     2281 gtatggtggc gacgatgccc ataggcgctg tcctcggctc tctgttctcc aacccgagca
     2341 gcctgggctt cgtctttgtg ccgatgatgg ggctcatcgc catttcgggg atcctctcca
     2401 ccaccccgtt cccgcgcatc ggaagtggcg agcgttcggc atgatccctg ccgatggcaa
     2461 gtctagtagc cgagtacggg aaggctagat cgaggagccg ccggtaagat tgcggccgct
     2521 ccggatcgtg gtgaggtttc tccgctacct actttacgat gccttgctct tcgaggtact
     2581 cctttgcaac gtcctcgggg tcctcctgct cgatgtccac ctgggcgttg agctgccgca
     2641 tgatgtcggc gtccagggac tcggaaaccg agttgagaac ctcttccatc tccggattct
     2701 cctcgaggac gtcgcttctg acaacgggtg cgggataata gaagggccag atattcttct
     2761 gatcctccat taccacgagc tcgtcggagg cgagctggct gtcggtggtg aaacccacac
     2821 cggcgtcgcc ctcgccttgc agtacgccct ggtagatcgg gccgccgatg ctgttcacga
     2881 ccttgacgtc cccgaactct aggtcctcgt agtttttctt catgttcggg acgccgtccg
     2941 aacgcttcag gaactcggag aaacagacaa aggtgatgtc acccgaggcc tcagcgaggt
     3001 ccgcgagggt ctgcaggttg tactcctcgg cgacctccct gcgcacgaag atgccgtagg
     3061 tgttgttgaa cggcgctggc tcgagcatcg tgttcgccgg gtcgcgatca gcgtaggctt
     3121 tctttgcttc ctggtaggtc gcatcgggag tatccggttg ttccatttgc ttgaagatct
     3181 ccacgagggc cgtccccgtg tactcggggt acatgtcgat ctgaccgttc tggagggcct
     3241 tgtcggctat cgccgaggag ccgaggtcta gcttcgtctc cacgctgaag ccgttcgccg
     3301 cgagcgcctg ggcgtatagc tcgcctagaa cgtactgctc ggtgaagttc ttggagccga
     3361 cggtaatcgt tgggccgcct ccaccgctgt cgtccccggc gcttggtccc gagccgctgc
     3421 tgctgccgac gttgccgcag ccggcgaagg cgagcagcgc gagtgccgct agcagcgctg
     3481 gtatggcgcg gatcagcttc gttctgttct tcacccttcc tcctttgcct ttgtgcctaa
     3541 cgcccgcgct tctgcgcgat cttcaacccc ttcggggtca tagccctctc cagcacgccg
     3601 aagccgacct ccgtgatgat ggccaaggcc gcaaccgaga acgagccggc gagcaatatg
     3661 gtcgtgtcgt tgagctgggt gccttccacg atcaattccc caagaccgcc gcccccgatg
     3721 aacgtcgcca aagtcgcgct cgcgaccacc tgcaccgcgg aggtgcggat gccggcgaag
     3781 atcagcgggg cggctatcgg gagctgtatg ctggatagga tctggctccc agagagcccc
     3841 atgccgcggg cggcgtcgat gacgtccttg ctgacctccc tgagtcccgt tgaggcgttg
     3901 atcaggatag gggggatggc aagagccgtg agcgcgacga gcgccgtgtc gaagccgaaa
     3961 ccgaggaagg gttggcagag cgccaggatg gcgagcgatg ggacggcgcg tccgatgttg
     4021 ccgatgttga cggccagcgc cgcgcccagg gaggtgttcc ttatcgcgag tgccacaggc
     4081 agcgcgatag cgatgccgat gagcagggcg gccgaggaga ggaggatgtg ctgcctgagc
     4141 agctcgaaga agtcgggacg tctaaggata tcgagtatcc cggacatcct acgtcctcct
     4201 gcgcgcccat ggcctcaggt agcgctcgag ggcgaggagc gcgagatcgg cgatgatggc
     4261 gaggacggtc gcgaggatgg ccccggtgat gatcatggtc gggaagctgc ggttgatgcc
     4321 gtcgaagatc attttcccca ggccgccacc ggcgatgtag gcgccgatcg tcgcgatccc
     4381 gattatcgtg acggtcgcta tgcggatgcc agcaacgatc acaggcagcg ccagcgggag
     4441 ctcgacccgg aagaggatct ggccgtccgt cagtcccatc ccgcgcgcag cgtctatggt
     4501 ctccgccggg acggcgtcga gccccgcgac ggtgttgcgg atcaggatga ggagcgagta
     4561 ggcgacgagc gcgatgatga cgggcgtcgg cccgatggtt atgccgggga tggagatgag
     4621 gataccgaag agggccaaac ttggtatgga gtagagtagc ccagtaacga aggtgacggg
     4681 cgggtaggcc ttgcggtagc gggagacgag cacgccagtt ggcagcgaga tcatgagcgc
     4741 tatcgcgacc gagacgaacg agaggaagat gtggccctgt atcgcaggca cgatgtcttc
     4801 ccagaagtta tccgagatcc aatcccactc tatgagcttt ggatcctcga tggacgagag
     4861 gtcctgtagc aacgccaggg cggggttagc cacgcgagtc gtcccgggcg ctggagagct
     4921 tctcgatggc gtctatagtg agcagaccct tttcgccgtc tctctcaacc acgcccttgg
     4981 tgctgccgga gccgatgatc tccgagagcg cgtcccgcac ggtcaggctg ccgtctatgc
     5041 ggggcagatc ctcggcttcg ccgttggccg gctcgagccg catgtcgccg actctgacga
     5101 gggagagccg cttgaggatg cggtcggtgc cgacgaacga ggcgacgaac tcggagttcg
     5161 ggttagcgag gatggtctcg gggatgtcgt actgggcaag gacgcctccc tgcttgagga
     5221 tggcgatctt gttccccatc tttatggcct cgtctatgtc gtgggtgacg aagacgatgg
     5281 tcttctttat atcttcctga atccgcaaga actcgtcctg gagccgctcg cgcgtgatcg
     5341 ggtcgaca
//
LOCUS       DASTKM010000358         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_693743, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000358 DASTKM010000000
VERSION     DASTKM010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693743"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFQ10_14215"
     CDS             <1..942
                     /locus_tag="VFQ10_14215"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9966544.1"
                     /translation="ATVDPDPEARRYVETTPGYGVLEEALASVDCDAVLVASPPGTHY
                     VVAKAALEAGKHVLCEKPLATNLQDAFDLVETADRAERVLMVSQNYRYNAPFRAVQRL
                     LMEDELGELASIEISCRRDTRMLFAPDDFRYSMRHPYVLDMSIHHFDLIRAATGRDVS
                     SVYAHSWRVPDSPFVHHPAVAAILDLEAGVPVIYQGDWATHEPETSWNGEWEIVGEAG
                     RILWSGSEEDRGIGEVVLERWGKEPRLIEQRDLEFVERGATLQALRAALEDGEPPETV
                     AADNVKSLAAMLGCVSSIESGEPVDLAALLAARGAKL"
     gene            995..1996
                     /locus_tag="VFQ10_14220"
     CDS             995..1996
                     /locus_tag="VFQ10_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET9966545.1"
                     /translation="MARPVTLFTGQWADLALEELARKCGEWGFDGLELACWGDHFDVD
                     KALSESGYIEGRWEILRSNGLNCWAISTHLVGQAVCDPIDARHGATLPPDVWGDGDPE
                     GVRRRAAEKVADTARAAARFGVTQVNGFTGSSIWHLLYSFPPNDFEEIERGYQDFAER
                     WNPIIDAFDEEGVRWGLEVHPTEIAYDFVTTRKALDAIGNREGFGINFDPSHFAHQFL
                     DSAAFIEEFADRIYHFHIKDSRKRLNGRRSILGGHLDFGSPERGWDFVSPGHGDVNLE
                     DCIRALNRIGYEGPLSIEWEDSGMDREYGARDALAFARRTDFEPSTQAFDAAFAREE"
     gene            1999..>2772
                     /locus_tag="VFQ10_14225"
     CDS             1999..>2772
                     /locus_tag="VFQ10_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9966546.1"
                     /translation="MERGEIGVGLVGYKFMGRAHSNAYRQVASFFDVDPAPRMAAICG
                     RDEVSVREAADTLGWDSYETDYRKLIARDDIDLVDVVTPGNTHREIVLTALEAGKHVL
                     CEKPLANTLAEAREMLTAGRNAGTVNMVCFNYRRAPAVQLARKLVEEGRLGEIRHWRA
                     TYLQDFIMDPSFPLIWRLQKEVSGSGALGDIGAHIIDLAHFVVGPISEVTGMTETFVE
                     ERPLEEPGAGGGLSASGGTQMGEVTVDDAAAFLARFENGA"
BASE COUNT          537 a          853 c          939 g          443 t
ORIGIN      
        1 gccaccgtag accccgaccc tgaagcccgt cgttatgtag aaaccacccc tggctacgga
       61 gtcctggagg aggcgttggc aagcgtcgat tgcgacgcgg tgctggtggc ttctccgcca
      121 ggcacgcact acgtggtggc gaaggccgcg ctcgaggcag gcaagcacgt cctctgcgag
      181 aagcctctgg ccaccaacct ccaggacgcc ttcgacctgg tcgagaccgc agacagagcg
      241 gagcgtgtcc ttatggtcag ccagaactac cgttacaacg ctccgttccg cgccgtgcaa
      301 cggctcctga tggaagacga gctcggggag ctcgcttcga tcgagatatc ctgcaggcgc
      361 gacacgcgga tgctcttcgc ccctgacgac ttcaggtact ccatgcgcca cccttacgtc
      421 ctcgacatgt ctatccacca cttcgatctc ataagagcgg cgaccggacg tgacgtgagc
      481 agtgtgtacg cccatagctg gcgcgtccca gatagcccgt tcgtccacca ccctgcggta
      541 gccgcgatcc tggatctcga ggccggtgtg cccgtcatct accagggaga ctgggcgacc
      601 cacgagcccg agacctcatg gaacggagag tgggagatcg tcggtgaggc cggacggata
      661 ctctggagtg gcagcgaaga ggaccgtggg ataggagagg tggtcctcga gcggtggggc
      721 aaggagccac gtctcatcga acagcgcgac ctcgagttcg tggagagggg ggcgacgctg
      781 caggcgctgc gcgcggcgct cgaggacggc gagccgcccg agactgttgc cgcagacaac
      841 gtgaagagcc tggccgccat gcttggttgc gtaagttcca tcgagagcgg cgagcctgtc
      901 gacctggccg cgcttctggc cgcgaggggc gctaagctgt agtggcgggt cggagtcggt
      961 ccgcgaggtt gagagaataa gaggaggttg aaacgtggcc agaccggtaa cgctttttac
     1021 gggacagtgg gctgaccttg cgctggaaga gctcgccaga aagtgcgggg agtggggttt
     1081 cgacgggctc gagctcgcgt gctgggggga tcacttcgac gtagacaaag ctctttcgga
     1141 gagcggttac atagagggac gctgggagat actgcgaagc aacggcctga actgctgggc
     1201 cataagcacc cacctggtcg gccaggcggt ctgcgacccc atagacgccc gccacggggc
     1261 gacgctacct cccgacgtgt ggggcgacgg cgacccggaa ggcgtgcgcc ggcgtgccgc
     1321 tgagaaggtc gcggacactg ccagggccgc ggcccgcttt ggggtaacgc aggtcaacgg
     1381 ctttacggga tcgagcatct ggcacctgct ctactcgttc ccgcccaacg acttcgagga
     1441 gatagagcgc ggctaccagg acttcgcgga gcggtggaac ccgatcatcg acgccttcga
     1501 cgaggagggc gtcaggtggg gactcgaggt gcaccccacg gagatcgcct acgacttcgt
     1561 gacaacccgc aaggccctcg acgccatcgg caaccgcgag ggcttcggta tcaacttcga
     1621 cccgagccac ttcgcccacc agttcctcga ctccgctgcg ttcatcgagg agttcgcgga
     1681 caggatctac cacttccaca tcaaggacag ccgcaaaagg ctgaacggcc gcaggagcat
     1741 actcggaggc cacctggact tcggcagccc ggagcgcggc tgggacttcg tctccccggg
     1801 ccacggcgac gtgaacctgg aggactgcat aagggcgctg aaccgtatcg gctacgaggg
     1861 ccctctcagc atcgagtggg aggactcagg gatggaccgc gagtacgggg ccagggacgc
     1921 gctagctttc gcgagacgta cggatttcga gccgtcgaca caagccttcg acgcagcctt
     1981 cgcgagggag gaataatcat ggagcgggga gagatcggag tcgggctcgt cggctacaag
     2041 ttcatgggcc gcgcccactc caacgcctac cgccaggtcg caagcttctt cgacgtcgac
     2101 ccggccccaa ggatggccgc gatctgcggc cgcgacgagg tcagcgtgag ggaggcggcg
     2161 gacactttgg gctgggactc ctacgagacg gactaccgca agctcatagc gcgcgatgac
     2221 atagatctcg ttgacgtcgt cacgccgggc aacacgcacc gggagatcgt gctcactgcc
     2281 ctcgaagcgg gcaagcacgt cctttgcgag aagccgctgg ccaatactct cgctgaagcc
     2341 cgcgagatgc tcaccgcggg acgcaacgcg gggacggtca acatggtctg cttcaactac
     2401 aggcgcgccc ccgcggtgca attagcgcgg aagctcgtcg aggaagggcg actcggggag
     2461 ataagacact ggcgggccac gtatttgcag gacttcatca tggacccttc tttccccctg
     2521 atctggcgcc tccagaaaga ggtctccggt tccggggccc tcggcgacat aggggcgcac
     2581 atcatcgacc tggcccactt cgtagtcgga cccatctctg aggtcacggg catgacggag
     2641 acgttcgtag aggagcggcc cctggaggaa cctggcgcgg gcgggggact atcggcgagc
     2701 ggcggcacac agatgggaga ggtaaccgta gacgacgcgg cggcctttct ggcccgcttc
     2761 gagaacggcg cc
//
LOCUS       DASTKM010000359         9737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1401944, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000359 DASTKM010000000
VERSION     DASTKM010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401944"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1796
                     /locus_tag="VFQ10_14230"
     CDS             <1..1796
                     /locus_tag="VFQ10_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966547.1"
                     /translation="QTLNNSAFEVATNANLVVNTSGCFDWLTGGAGTQMRPGVTVKND
                     TASGSGDESFGNGTKEDTAVPSIVTGGIPLNKSDLKAFGIFTEQGEVTPANPTGKYLA
                     LFWTRVQDPSGTTNMDFELNQSSTASANGVTPVRTTGDKLITYDLSNGGTVATISIRT
                     WSGSAWGSPQVISGSNSGARGSVNTTQIPSGSSGGLGTLDPRTFGEATIAFNQLFQGG
                     TCGKFGSAYLKSHSSDSFTAALKDFVPPTAVSISNCPATLTTDATDSVTLGNGISDTA
                     TLDVPANTNGSITFRLYGPFATAGEITASSCTAGKLISGATSTKQVTNFNPANPTYTS
                     DNFTPTVAEFYNWTAQFTSTTAGVNSTGEIGCGVAAEKSQVNKAGPAIVTEAQTPVTV
                     GATINDPATLSGGINPTGTITFKLYASQADCENDANALFEDPVDVDNGNADYTSGNYT
                     TTTVGNFFWKASYSGDNNNSAVSGVCGEDGETSVVGKAPSTLNTEQSFYPNDTVTVTA
                     TDGGPPTGDVTFSLFDNSDCSGTALYGPVSDTLDANGEASTNNTSFAVSTSGTLYWKV
                     EYEGDNSHEPVDSCSENTELTIDNGGPATSQ"
     gene            2331..5033
                     /locus_tag="VFQ10_14235"
     CDS             2331..5033
                     /locus_tag="VFQ10_14235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9966548.1"
                     /translation="MLSIRHTSPLLRYGVAVLAVGVAFAIKLLLDPLIVQETPFLLIF
                     GAIMISAWYGGLGPGLLATVAAGLATDYFFLQPTSSFSGFSLETVPLLAFFLEGTLVC
                     LLVEALRAARSRAEGSTLEAERQREHLRRNEEHFRSLVEGMRNYAIFTLDPQGCVASW
                     NAGAERLEGYNSEEIVGERFSVFFTEEDTRLGKPEEQLEAAASEGRSVEENWLVRKDG
                     SRFWADTILTALHDDEGELRGFSVVTQDITEKKEAERNLEEAEGRLRTLVEHVPAITY
                     TGEVGGDHALAYVSPQIEEVLGYSPGEVMADPDHWTKMLHPHDRKWVLAEERRTGETG
                     DPLALEYRMFARNGRVVWLRDEAMLVRDEEGHPLHWQGFMLDVTKRKKAEERLRESEE
                     LYRNVVEQAAENIFLMDPYNKHILQANASFHHSLGYEAEELRRLTLYDIVAHDHESID
                     RNVQRVLDRGRLSIGERRYRRKDGSLINVEVSAGAIAYGGRPALCVVAHDVTQRKMAE
                     EALRRSLDALLALYETGQILSSSLEREEIGSRLLQVVRRVSNLTAAVISIPDENGELS
                     IWRSSGLDGLWRQARFAPEALSARREVLESREHRLLRLRRPEDPRGRHLVSLYLPLLV
                     RDRIVGVLEAYGPEALGEEGSETLISLANQAASALENARLYAELTERENQLRHLIGKL
                     ITAQEEERRRVAHDVHDGLAQTAAAAHQHLQAFARHNPPGSARGQEELDEALQLVREV
                     VGEARGVIHDLRPTVLDDFGLAAAVRLQVQTLRSEGLEVGLEEALGDERLPPEVETTL
                     FRVAQEALANVRKHARSSTVRVVLDRSGRAVRLMVRDEGRGFIPDEAARSNGPGERVG
                     LSGMRERLSLLGGRFELQSEPGSGTTVTAEVEVPTNREDSDDAG"
     gene            5023..5727
                     /locus_tag="VFQ10_14240"
     CDS             5023..5727
                     /locus_tag="VFQ10_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9966549.1"
                     /translation="MQDEIKRLVLADDHHLLRRGFRSLLSNEPGLEVVGEASTGLEAI
                     EMCRRLAPDLVLMDVRMPEMDGITATRRIKREQPGVSVLMVTMHENPDYLLEALDAGA
                     AGYVLKDAPADRLINAVRRTLDGESPLNQELATRLLRQLAEEKQHNLYRAPSKRHEPL
                     DDALTPRETEVLGLLTTGQTNQQIAQTLTISKGTAKVHVERIIRKLGVSDRTQAAVRA
                     IELGFTSLETRRQPYS"
     gene            5852..6214
                     /locus_tag="VFQ10_14245"
     CDS             5852..6214
                     /locus_tag="VFQ10_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966550.1"
                     /translation="MLKPAEGPATIAILGGDPVIGRALESLLASADYVSRFFSEFPEG
                     AAEPLGGAQIAIVLPASSSKRRDALMHLIKTTKSTKNLPVVELTTTPNESGHDNKLPW
                     PCSIEVLRRSIDEALQNS"
     gene            6535..7467
                     /locus_tag="VFQ10_14250"
     CDS             6535..7467
                     /locus_tag="VFQ10_14250"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HET9966551.1"
                     /translation="MTEERGAVSGMDPAELWRQWMESGSRMWSGMSGGAQGSQVDPYG
                     MYRRWLEGIREMQERTIGAVPEPVAGNPEVHGLWQRWFELSADSWKKSAEMGQNAMEL
                     LPRWMQMLDEARTNLLAGGAPPSDPLQLATQWYNATSGPFSDFVGDVIEREEFLEPSS
                     RFLQHYASFYKVFKRNSEDYLKNLQLPVRSDITRIAGLVVNLEEKVDRIEEVLEDFEY
                     GYAEPATTESVKELETRLDRVEGKLDRLLAVLEGGTQDGGPQAETNGSGGEIRATDAA
                     RRKAREMGVDLAEVVGSGAGGQVTVEDVRKKGES"
     gene            7468..8568
                     /gene="phaC"
                     /locus_tag="VFQ10_14255"
     CDS             7468..8568
                     /gene="phaC"
                     /locus_tag="VFQ10_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HET9966552.1"
                     /translation="MTETANYGAVPTSGDAAIPADVENFFDKYSRGMKIVVEGAQADT
                     GQTPKETVWTKNKANLYHYAPNAQKKHPVPILMVYALINRPYILDLMPGNSFIEYLVG
                     EGFDVYMLDWGIPGDEDKDLTFENYVLDYLPRAVKKVLRHSHSDEFTMLGYCMGGTMS
                     AMYASLFPEHLKNLILMTAPVEFPEGEMGLYSLFTSEKYMNPGLLADAFGNIPGDLID
                     TGNRMLKPVTNYVGTYVNMWERIFEEKPMETWLAMNKWVNDGPPFPGAAFRQWITEFY
                     QQNKLARGEMVLRGRRIDLSNIKVPLLNIAGKKDHICTLPQAEATMNLVGSEDKEFFV
                     LEAGHVGLMTGRGAKKGLWPKVSDWLSTRSGG"
     gene            8923..>9737
                     /locus_tag="VFQ10_14260"
     CDS             8923..>9737
                     /locus_tag="VFQ10_14260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9966553.1"
                     /translation="MKLGVQLNSFDWTGGPGRFGRNLADIGKTAEDAGFDRIGVADHV
                     WQHPIMGGPEADEPECYTTLAFLAANTQRVKLTAMVSGVHFRHPAVLVKAVTTLDVLS
                     GGRACFGVGSGHYEEETLGLGIPFPPQRERFEMLEETIQIALRMWSGERGDERPFEGN
                     HYRLGRPLNLPQSISRPHPPIMIAGDGEKKTLRLVARYADACSLRPGPQVPRKLDVLR
                     RHCEEEDTDYDSIEKTCAFAFDVGERGEKVDELIGQLRWLSGMGIETVIGFVPR"
BASE COUNT         2081 a         2935 c         3109 g         1612 t
ORIGIN      
        1 cgcaaacctt gaacaacagc gcgttcgagg tggccactaa cgccaacctg gtcgtcaaca
       61 cctcagggtg cttcgactgg ctcacgggcg gagccgggac ccagatgcgt ccgggtgtaa
      121 cggtgaaaaa cgacaccgcg agcggatccg gggacgagtc cttcgggaac ggcaccaagg
      181 aagacaccgc ggttccgagc atcgttaccg gtgggatccc gctgaacaag agcgacctga
      241 aggcgttcgg catcttcacc gaacagggcg aggtgacgcc agcgaacccg accggcaagt
      301 accttgcgct gttctggacg cgcgtgcagg acccatcggg caccaccaac atggacttcg
      361 agctcaacca gagctctaca gcctcggcga acggcgtgac ccccgtgagg actactggcg
      421 acaagctcat cacctacgac ctctccaacg gcggaaccgt ggcgacgatc tccatccgca
      481 cgtggagcgg ctcggcgtgg ggctcaccgc aggtgataag cggctcgaac tcgggcgcgc
      541 gtggttcggt caacacgacg cagattccgt ctggttcgag cggcggcctc ggtacgctcg
      601 accctcgtac tttcggcgaa gccacgatcg cgttcaacca gttgttccag gggggtacat
      661 gcggtaaatt cggctcggcc tacctcaaga gccattcgtc cgactcgttc accgccgccc
      721 tcaaagactt cgttccgccc acggcagtta gcatctccaa ctgtccggca acgctgacca
      781 ccgatgcgac cgacagcgtg acgctcggta acgggatcag cgacaccgca accctcgacg
      841 tgccggctaa cacgaacggc tcgatcacgt tccgtctgta cggtccgttc gcgaccgcag
      901 gagagataac tgcgtcgagt tgcacggcgg gcaagctcat cagcggtgcc acgtcgacca
      961 agcaggtcac caacttcaac ccggccaacc caacctatac ttcggataac ttcacgccca
     1021 ccgttgcgga attctacaac tggaccgcgc agttcacctc taccacggcg ggcgtcaact
     1081 cgacagggga gatcggatgc ggtgttgcgg ctgagaaaag ccaggtgaac aaggctgggc
     1141 cagctatcgt aaccgaggct cagacgccag ttaccgttgg cgcaacgatc aacgaccccg
     1201 ccacactcag cggtggcatc aacccgactg gcacgatcac cttcaagctg tacgctagcc
     1261 aagccgactg cgagaacgac gccaacgcgc tcttcgagga cccggtcgat gtcgacaacg
     1321 gcaacgccga ctacacctca ggcaactaca ccacgacgac tgtgggcaac ttcttctgga
     1381 aggccagcta cagcggcgat aacaacaaca gcgccgtctc cggtgtgtgc ggtgaagatg
     1441 gcgagacgag cgtagtcggt aaggcaccat caacgttgaa cactgagcag tcgttctacc
     1501 cgaacgacac cgtcactgtg acagcaaccg atggcggacc accgactggt gacgtcacgt
     1561 tctcactgtt cgacaactcc gattgctccg ggaccgcgct gtacggtccg gtgagcgaca
     1621 ccctggacgc caatggtgag gccagcacga acaacacctc gttcgcggtc tcgaccagcg
     1681 gcacgctcta ctggaaggtc gagtacgagg gtgacaacag tcacgagccc gtcgattcct
     1741 gcagcgagaa cacagagttg actatcgaca acggaggacc agccaccagc caatagtcaa
     1801 ttaatcactc gtgaggttcc ggcccgctgg agccggggcc agcgaacacc taatacgagg
     1861 aggtatgaag acgctgaggc ccgctcgatg agcgggcttc agcctttggc ggaaatctta
     1921 ccgaccgggc ttactcatac gtaatctggt agatccctct ggttggaggg ctgagatccc
     1981 cgtgcagtgg attcccgctt gcgcgggaat gactggagaa gaggcgggga tgatgagagg
     2041 agaggggaat gaggtactct ctcatgctgg gaggaaacgg gtaggtgtag aaacccaagt
     2101 agcaacgaag tggtaaacgc caaggagggc tggtctggcg aggtgtgatc cgacgcccag
     2161 gaccaccgta gtgagtaata aacattccaa aatactgcat ttggggtatg acctaggcct
     2221 cgcgaaggcg caaaatggta tcgtgagtgt aaggagaggt gagaatggga accgcaatat
     2281 gcacgctgag gggtaatcgt ccctctgctt tcgacgggta cacggtctag atgctctcta
     2341 taagacatac gtcgccgttg ttgcggtacg gcgttgccgt gctggcagtc ggcgtggcgt
     2401 ttgcaatcaa gctgcttctc gacccgctga tcgtgcaaga gacccctttc ctgctgatct
     2461 tcggcgccat aatgataagc gcctggtatg gcggccttgg acccggactg ctcgccacgg
     2521 tcgctgcggg cctggccact gactactttt tccttcaacc gaccagttcc ttctccgggt
     2581 tcagcctgga gaccgtgccg cttctcgcgt tcttcctgga agggacgctc gtctgcctgc
     2641 tagtcgaggc cctgcgcgcc gcgaggtcgc gggcggaggg tagtacgctg gaggccgagc
     2701 gccaaaggga gcacctgcgc aggaacgagg aacacttccg ctcgctggtg gaaggcatgc
     2761 gaaactacgc gatcttcacg ctcgacccgc aggggtgcgt ggcaagctgg aacgcgggtg
     2821 ctgaacgtct cgaaggctat aactctgaag agatcgtcgg agagcgcttc tccgtgttct
     2881 tcaccgaaga agacaccagg ctaggcaagc ctgaggagca actcgaggca gcggcgagcg
     2941 aagggcgctc cgtagaggag aactggctcg tgcgcaagga tggatctcgg ttctgggcag
     3001 acacgatcct gacagcgttg cacgatgatg agggcgaact gcggggtttc tcggtggtca
     3061 cgcaggacat cacagagaag aaagaggcgg agcggaacct cgaggaggcc gagggccgtc
     3121 tgcgcacgct cgtcgaacac gtgccggcga tcacctacac cggggaggtt ggcggagacc
     3181 atgctctagc ttacgtcagt ccgcagatcg aggaggtgct cggctactca ccaggggagg
     3241 tgatggcaga tcccgaccac tggacgaaga tgttgcatcc ccacgacaga aagtgggtgc
     3301 tggccgagga gaggcgtacg ggtgagacgg gtgatccgtt ggcgctggag tacaggatgt
     3361 tcgccaggaa cgggcgcgtt gtctggctgc gcgacgaggc aatgctcgtg cgcgacgaag
     3421 agggtcatcc acttcactgg caggggttca tgctcgatgt caccaagcga aagaaggcgg
     3481 aggagagact cagagagagc gaggaactgt accgcaacgt cgtcgagcag gccgccgaga
     3541 acatctttct gatggacccg tacaacaaac acatccttca ggcgaacgcc tcgttccacc
     3601 actcgctcgg ttacgaggcc gaggagcttc gccgcctgac gctctacgac atcgtcgccc
     3661 acgatcacga gagcatagac cgcaatgtcc agcgcgttct ggatagagga cgcctctcta
     3721 tcggtgagag gcggtacagg cgcaaggacg gctctctcat caacgtggag gtcagcgccg
     3781 gcgctatcgc ctacggcggc cgcccagcgc tctgcgtcgt cgcccacgac gtgacgcaac
     3841 gcaaaatggc cgaagaagcg ctgagacgta gcctggacgc cttgctcgcc ctctacgaga
     3901 caggccagat cttgagttcc agcctggagc gcgaagagat cggctcccgc ctgctgcagg
     3961 tggttcgccg ggtctcgaat ctcaccgccg cggtcatcag tatcccagac gagaacgggg
     4021 aactgagcat ctggcgttcc tcgggactgg acgggctgtg gcgccaggct cgcttcgccc
     4081 ccgaggccct atccgcccgt agggaggtct tggagagtag ggagcacagg ctcctcaggc
     4141 tgcggcggcc ggaagacccg cgagggcggc acctggtgag cctgtacctg cctttgctgg
     4201 tccgtgaccg gatcgtcggt gttctcgagg cctatggtcc cgaagccctg ggcgaagagg
     4261 ggtcggagac cctgatcagc ctcgcaaacc aggctgcaag cgccctggag aacgcgcggc
     4321 tctacgcgga gctgaccgag cgtgagaacc agctcaggca cctgatcggg aagctcataa
     4381 cggcccagga agaagagcgt cgccgcgtcg cccacgacgt ccacgacggc ctggcccaga
     4441 ctgcggcggc cgcccaccag caccttcagg cgttcgcccg tcataatcct cccggttctg
     4501 ccaggggcca ggaggagctc gatgaagcgc tccagctcgt cagggaggtg gtcggagagg
     4561 cgcggggggt gatccacgac ctgcgtccca cggtgctcga cgactttggc ctggccgccg
     4621 ccgtccgtct tcaggtccag acgctccggt ctgaaggcct ggaggtcggc ctggaagaag
     4681 cgcttggcga tgagcgtctg ccacccgagg tggagacgac gctcttccgc gtcgcccagg
     4741 aggcgctggc gaacgtacgc aaacatgcgc ggtcctccac ggtgcgcgtg gtgctagacc
     4801 gctcgggaag agcggttcgc ttgatggtca gggacgaagg acgcggcttc ataccggatg
     4861 aggcggcgag gagtaatggt cccggcgaga gggtcggcct ctccggcatg cgcgagaggt
     4921 tatccttact cggcgggagg ttcgagctcc agagcgaacc aggatccggg acaaccgtaa
     4981 cggcggaggt cgaagtgccg acgaatagag aggactctga cgatgcagga tgagataaag
     5041 cggctcgtcc tcgccgacga tcaccacctt ctcaggagag gattccggag cttgctcagc
     5101 aatgagcccg gactggaggt ggtcggcgaa gcgtcgaccg gcctcgaggc catcgaaatg
     5161 tgccggcgtc tggcgccgga tctcgtactt atggacgtcc gcatgcccga gatggacggc
     5221 atcacggcca ctcgccggat caaacgcgag cagcccggcg taagcgtact catggtgact
     5281 atgcacgaga atcccgacta cctgctcgaa gcactggatg ccggcgccgc cggctacgtg
     5341 ctcaaagacg cccccgcaga ccgcctgatc aacgcggtcc gcagaactct ggacggcgag
     5401 tctccactca accaggaact ggccacgcga ctcctcaggc aattggccga agagaaacag
     5461 cacaacctgt atcgtgctcc gtcaaagcgc cacgagcctc tggacgacgc gctgacgccc
     5521 cgcgagacgg aagtcctcgg gctcctgacc accgggcaaa caaaccagca gatcgcgcag
     5581 acactgacca taagcaaggg gacggccaag gtgcacgtgg agcggataat ccgcaaacta
     5641 ggggtctcgg atcgcaccca ggcggccgtg cgcgctatcg aattgggatt cacttctctc
     5701 gagacaaggc ggcaacctta ttcctgaaga ttaggtttca ggttcgtatg ccgagtggca
     5761 tactgaagat aggccatcca gcgtacgcgc ggcccttctc tgccacatat aattagcgaa
     5821 tgaacttagt catgggctcc gggaactgag catgctgaag cctgccgaag ggccggctac
     5881 catcgccatc ctgggcggcg accccgtgat cggcagagct ctggaatcgt tgctcgcctc
     5941 cgccgactac gtgtcacgat tcttttccga gtttccagaa ggagccgcag aaccgctcgg
     6001 aggggcgcag attgcgatcg ttttgccagc ctcgagtagc aagcggcggg atgccctcat
     6061 gcacctgatc aagaccacca aatcgacgaa gaacctgccc gtggtcgagt tgaccacgac
     6121 cccgaacgag agcggccacg ataacaagct gccctggccc tgcagcatag aagtcctgcg
     6181 gcgctctatc gacgaggcgc tgcaaaacag ctaatcagca agtcgcccgg cttcgcgcca
     6241 ggcgacttgt cagcacgcgc cctgtcgggc gcttgtcagc acttcagcca gtaagcactt
     6301 cagcattcgc aagcccttgc tgacttgctg aaagccgccg actgaaggga ggcggcgtgc
     6361 tgacaagcga aggcgaagcc tgagcgtgct gaaatgctga catgctctag tatatgccgt
     6421 taacacataa gtttcggttg cttgtataat tcgcttgatc ggcactcgct ccggtgagag
     6481 cgaggtgggt gccgaggact ggcattgttt ggtggcatga cgaggaggtt ttcggtgacg
     6541 gaggagagag gagcggtgtc cggcatggac cctgccgagc tctggcgcca gtggatggag
     6601 tcggggagca ggatgtggtc ggggatgtcc ggcggcgccc aggggagcca ggtcgacccc
     6661 tatggcatgt accgccggtg gttggagggc atacgagaga tgcaggagcg gacgatcggg
     6721 gcagtacccg agcctgtcgc tggcaacccg gaggttcatg gcctctggca gcggtggttc
     6781 gagttgagcg cagactcctg gaaaaagagc gccgagatgg gccagaacgc gatggagtta
     6841 ctgccgcgct ggatgcagat gctcgacgaa gcgcgtacca acctgctggc tggaggtgcg
     6901 ccgccgagcg accctttgca gctcgccacc cagtggtaca acgccacgag cggccccttc
     6961 tcggactttg tgggagacgt catcgagcga gaggagttcc tcgagccttc gagccgcttc
     7021 ctccaacact acgccagctt ttacaaagtg ttcaagcgca actccgagga ctacctcaag
     7081 aacctccaac tccccgtccg ctccgacatc accaggatcg ccgggctcgt cgtgaacctg
     7141 gaggagaagg tggaccgcat cgaagaggtc ctcgaggact tcgagtacgg ttacgccgag
     7201 cctgcaacga cagagtccgt caaggagctc gagacacgac tcgacagggt cgagggcaag
     7261 ctcgatagac tgctcgcggt gctagagggc ggtacccagg acggtggtcc gcaggcggag
     7321 accaacgggt caggcggcga gatcagggcc acagacgccg cgaggcgcaa ggcgcgtgag
     7381 atgggcgtcg acctggctga ggtagtcgga agcggcgccg gcggacaggt caccgtcgag
     7441 gatgtgcgca agaagggaga gagctagatg accgagacgg ccaactacgg cgcggttcct
     7501 acctcgggcg atgccgccat accggcggac gtggagaact tcttcgacaa gtacagccgc
     7561 gggatgaaga tcgtcgtcga gggcgcccag gccgacacgg gacagactcc caaggagacg
     7621 gtctggacca agaacaaggc gaacctctac cactacgccc ccaacgccca gaagaagcat
     7681 cccgtaccca tactcatggt ctacgccctc atcaacaggc cttacatact cgacctcatg
     7741 ccaggcaaca gctttatcga gtacctcgtc ggcgagggct tcgacgtcta catgctcgac
     7801 tggggcatac ccggcgacga agacaaggac ctcaccttcg agaactacgt cctcgactac
     7861 ctgccgaggg ctgtaaagaa ggtcctcagg cactcgcact ccgatgagtt caccatgcta
     7921 ggctattgca tgggcggcac gatgagcgcc atgtacgcct ccctgttccc cgagcacctg
     7981 aagaacctca ttctcatgac cgcccccgta gagttccccg aaggcgagat ggggctctac
     8041 agcctgttta cgagcgagaa gtacatgaac ccggggctgc tcgccgacgc cttcggaaac
     8101 atcccaggcg acctgatcga cacgggcaac aggatgctca agcccgtgac caactacgtc
     8161 ggcacgtacg tcaacatgtg ggagcgtatc ttcgaggaga agccgatgga gacctggctc
     8221 gcgatgaaca agtgggtcaa cgacgggcct cccttccctg gcgctgcgtt caggcagtgg
     8281 atcacggagt tctaccagca aaacaagctg gccaggggcg agatggtcct ccggggccgc
     8341 cgcatcgacc tctcgaacat aaaggtcccg ctactcaaca tcgccggcaa gaaggaccac
     8401 atctgcaccc tcccgcaggc cgaggccacg atgaacctcg tgggcagcga ggacaaggag
     8461 ttcttcgtcc tcgaagccgg tcacgtagga ctcatgacgg gccgaggcgc caagaagggc
     8521 ctctggccca aagtaagcga ctggctaagc acccgctccg gcgggtagcg gaaggcgagg
     8581 ctacccgcgc ttgtcagcat ttcagcaagt cgtctgaggc cgccagaccc gaacgccgcg
     8641 gatggcggtg cctcgtgcgc ggccgggcga ggcctaaacg ggagaagctg acaagctgag
     8701 agcggggacc tttaggcccg tagcgtgctg acaaggcgcg aagcgccgtg ctgacaagcg
     8761 cggggtttgg aagggttgcc cttccaaact ccgctatgtc atccgtcacc cggaaaccac
     8821 gacgatcttc actaccggcc gccacgccgc gcccatacac tcctccgtag agcttcgaag
     8881 aagcgacaga ggaggcacga tgcaggacca gggcgggcag gcatgaagct tggagtacag
     8941 ttaaacagct tcgactggac cggaggaccg gggcggttcg gtaggaacct cgcggatatc
     9001 ggcaagactg cagaggatgc gggcttcgac cgcatcgggg tggccgatca cgtctggcag
     9061 cacccgatca tgggcggacc cgaggcagac gaaccggagt gctacacgac gcttgcgttc
     9121 ctggctgcca acacgcaaag agtcaagctc accgcgatgg tgtctggggt gcacttccgc
     9181 caccctgccg tcctggtaaa ggccgtcacg acgctggatg tgctctcagg cggtcgtgcc
     9241 tgcttcggcg tcggctcggg ccactacgaa gaggagacgc tggggctcgg tataccgttc
     9301 cctccacaga gggagcgttt cgagatgctc gaggagacga tccagatagc cctgcgaatg
     9361 tggagcggcg agcgtggaga cgagcgcccc ttcgagggga atcactaccg gctcgggcga
     9421 ccactgaacc taccgcaaag cattagcagg ccccaccctc cgatcatgat tgccggcgac
     9481 ggggagaaga agacgttgcg cctggtcgcg cgctacgccg acgcctgcag tttgcgcccg
     9541 ggcccccagg tacccaggaa gctcgacgta ttgcgcaggc actgtgagga agaggacacg
     9601 gactacgact ccatagagaa gacctgtgct ttcgccttcg acgtcggcga gagaggcgag
     9661 aaggtagacg agttgatcgg gcagttgcgc tggctctcgg gcatgggcat agagacggtg
     9721 atcggcttcg tgccacg
//
LOCUS       DASTKM010000360         5248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_697332, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000360 DASTKM010000000
VERSION     DASTKM010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697332"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFQ10_14265"
     CDS             complement(<1..596)
                     /locus_tag="VFQ10_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9966554.1"
                     /translation="MTPERSRIALVTGAGSGIGRAIAEKLAQNGERVVVNDLNGETAD
                     EVAAGIKESGGEAAAAPGDVSDAESVQRLVAATHEAYGFPEILVNNAGFLQQKRFVDL
                     TVEDFDRMISVHLRGTFLCTRAVLPEMLSRGSGIIVNVASQLGQIGGIELCHYSSAKA
                     GIIGLTKSLAREVSAQGVRVNAVAPGPINTELVLGLSE"
     gene            complement(692..2068)
                     /locus_tag="VFQ10_14270"
     CDS             complement(692..2068)
                     /locus_tag="VFQ10_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9966555.1"
                     /translation="MSERPSQPGEGSVGGTVEGIQTSFTTRELVYPSIIAFLAWVVCV
                     YDYIMFGTLLPKMADDFGWATATSTAIATLVSIGTFFVAILVGPMIDYVGRKRSLIIT
                     TAGTGLSSLLTGLTPGVFSAPYLTIVRSMSGLGYSEQAVNTTYLNELYGNRKSRGFIY
                     AFIQGGWPIGVLLASAFTALLLGTVGWRGVFMLATIPALIVAALGLRLKESPRFLAMQ
                     RVRQLEKEGRHEEAVEFGRHFDIDVHHVQETTLQQIFEPDIRRHTIFLSLAFLTNWIG
                     IQVFAVLGTTVLTEGKGVSFGSALTVLIISNAAAYIGYLCHGFVGDRIGRRLTIAGGW
                     VISGLAYSLMLFGPSSDIFVLTMYTIGLFFIIGPYAALLFFMGESFPTRVRGTGASFV
                     NAMGPIGAIAGGALLTAFLSVSGENMVLSAFLSGGVATILSGLLMLGTRNVTDPREAE
                     LVEGSTAT"
     gene            2477..3232
                     /locus_tag="VFQ10_14275"
     CDS             2477..3232
                     /locus_tag="VFQ10_14275"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET9966556.1"
                     /translation="MTREESLNGTRKARKSYEQIIAHVRGEISSGELRPGDRLPPETD
                     LARSLGVSRPTVREALKVLEALNVLESSTGPTGGTFVKSLDDVGVAEYLTDSITLLLD
                     TDELTLEELWSAREAIEILSVDMAAARRTEQDLAMMRRIIESDEDKDFEAYFPDITFH
                     RAIASASKNRLLSLFMLSICMTISTLSERYVLPEAKQVSQDQHRLIYEAILDQDEALA
                     RMRMKEHLQMAYEVYRQAVPRIMGDRITSQTRT"
     gene            complement(3761..4642)
                     /locus_tag="VFQ10_14280"
     CDS             complement(3761..4642)
                     /locus_tag="VFQ10_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9966557.1"
                     /translation="MTDLKKAAIFTEVSLMLAAVFWGANYAATKYAAEFMPPLLIVAL
                     RFTAGGSLMFCLLRILEPADRLKPKDLLSVAGLGCLGVAIGQTAFTFGVSMTSAANTG
                     LIFATAPIWGLFLSLMLGLERPTRRGMVGVGLSILGVGIVFYEGLGTEGTSLAGDLFV
                     LVAAMAFGSYTVLSMPVLERHSPLAVATYSLLFGGLVVLLLSSPYLTGLEWGSVGIGA
                     WAAVGFSAVFATAFSFSAWQTGVSRIGANRVLVYQYLITITGVASGIVFFSEVLGIEK
                     LVGGAVILVGIYLARRQ"
BASE COUNT         1096 a         1587 c         1609 g          956 t
ORIGIN      
        1 tcctcggaga ggccgaggac gagctcggtg ttgatgggtc cgggcgcgac cgcgtttacc
       61 cggacgccct gcgccgagac ctcgcgggcc agcgacttcg tcaggccgat gatccccgcc
      121 ttggcagagc tgtagtggca cagctcgatg ccgccgatct ggccgagctg agaggctacg
      181 ttgactatga tgccgctgcc ccgcgagagc atctcgggca gcacggcgcg ggtgcacagg
      241 aacgtaccgc gcaggtgcac cgagatcatc cggtcgaagt cctcgaccgt caggtcgacg
      301 aaccgcttct gctgcaggaa tccggcgttg ttcaccagga tctcgggaaa accgtaagcc
      361 tcgtgtgtcg ccgccacgag ccgctgtacg gactccgcgt ccgatacgtc gccgggagcg
      421 gctgccgctt ctccgcccga ctcctttatc ccggcggcga cctcgtcggc ggtctcaccg
      481 ttcaggtcgt tgacgacgac tcgctcgccg ttctgcgcga gcttctcggc gatggcgcgg
      541 ccgatcccgc ttcccgcccc ggtaaccagg gcgatccggg agcgttcagg cgtcatcgac
      601 gccctccttt cgctgacaca ttactacgaa ggggcgcgcc ggatccggcg cccctttgct
      661 actttcctca acgttcgaaa cgcgggtcac cttacgtagc ggtagaaccc tccaccaact
      721 ccgcctcgcg cgggtcggtc acgttccggg tgcccagcat taagagcccg gagagaatgg
      781 tggccacacc gccgctgagg aaggcggaga gcaccatgtt ctccccgctc acagagagga
      841 acgcggtgag caacgcccct ccggcgatcg ccccgatcgg gcccatcgcg ttcacgaacg
      901 aggcgcccgt gccgcgcacc ctggttggga acgactcgcc catgaagaac agcagggccg
      961 cgtagggtcc gattatgaag aagagcccga tcgtgtacat cgtcagcacg aagatatcgg
     1021 aactcggtcc gaacagcatg agcgagtagg cgagaccgga tatcacccag ccgccggcga
     1081 tggtgaggcg ccggccgatg cgatcgccca cgaacccgtg gcagaggtag ccgatgtaag
     1141 cggccgcgtt ggagataatg agaactgtca gcgcgctccc gaacgagacc cctttgccct
     1201 cggtgagcac ggtggtcccg aggacggcga agacctgaat gccgatccag ttggtaagga
     1261 aggcgaggga gaggaagatc gtgtgccgcc ggatgtccgg ctcgaagatc tgctgcaggg
     1321 tcgtctcttg cacgtgatgc acgtcgatgt cgaagtgccg gccgaactcg acggcctcct
     1381 cgtgccggcc ctccttctcg agttgccgga cccgctgcat ggccaggaac ctgggcgact
     1441 ccttgagcct gaggccgaga gccgcgacta tgagcgccgg tatggtggcg agcatgaaaa
     1501 cgccgcgcca ccctacggtc cccagcagca gcgccgtaaa ggccgacgcg agcagtacgc
     1561 cgatcggcca cccgccctgt atgaaggcgt atatgaaccc ccggctcttc cggttaccgt
     1621 agagctcgtt gaggtaggtg gtattcaccg cctgctcgga gtagccgaga ccggacatgg
     1681 atcggacgat cgtcaggtaa ggcgccgaaa acaccccagg ggtcaatccc gtcaggaggg
     1741 agctaagacc tgtgccggcc gtggtaatga tcagcgacct ctttcgaccg acgtagtcga
     1801 tcatcggccc cacgaggatg gctacgaaga aggtcccgat cgacacaagg gtcgcgatcg
     1861 cggtgctcgt cgcggtcgcc cagccgaagt catccgccat cttcggcaga agcgtcccga
     1921 acattatgta gtcgtagacg cagacaaccc aggcgaggaa agctatgatc gaggggtaga
     1981 ccagttcacg cgtagtgaag gaagtctgta tcccctctac cgtccctccg acagagccct
     2041 cacccggctg acttggtcgt tcactcatct caaccgtcct ccttgatcca acagcccatg
     2101 gtccagtggc ctagcgccga acgccgccct acgtcgctaa accccaaaca cccaagaggc
     2161 acgatagcca aacgccgata atttgtcaaa caaatactcc tgcaaactgc tcgtcccgtg
     2221 ccccgaagtg gccaggtgct tcatcgccgc aggaaaggcc gggtggaagc cgagaatctc
     2281 gctgcacctg gtgttgagcg cgccgccgtg ctggagatcc atcgcccatt cattcgtgct
     2341 tcatcggtgt ctggtctcat ggagcgactc gtgggacgga gggagaagcg gcaaagacaa
     2401 aaatgcggtg ggcttgtaca gtaacgaagc acttacctta caattagtgt tgcaccactg
     2461 cgaagggatc tgtggcatga cgagagaaga gagcctgaac ggaacgcgga aggcacgcaa
     2521 gtcctatgag caaataatcg cccatgtacg cggcgagatc tcttccgggg agctgcgtcc
     2581 gggcgacagg ctgcctccgg agacggatct ggcgcgctcc ctcggggtca gccggccgac
     2641 ggtgcgggag gctctcaagg tacttgaggc gctcaacgtg ctggagagct cgacgggacc
     2701 cacaggggga acattcgtca agtcgctaga cgacgtcggg gtagcggagt acctgacgga
     2761 ctctataacc ttgctgctcg acacggacga gttgacgctg gaggagctgt ggtcggccag
     2821 ggaggccata gagatcctgt cggtggacat ggccgccgcc cggcgaacgg agcaggatct
     2881 cgcgatgatg cggaggatca tcgagagcga cgaggacaag gactttgaag cttacttccc
     2941 ggacataacg ttccacaggg ctatcgcgag cgcttccaag aacaggctgc tgagtttgtt
     3001 catgctatct atttgtatga cgatcagtac gctgtccgag cgatacgtct tgcctgaggc
     3061 gaagcaggtc tctcaagatc aacatcgctt gatctacgag gccatcctcg accaggacga
     3121 ggcgctggcc cggatgcgga tgaaggaaca cctacagatg gcttacgagg tctaccgtca
     3181 ggccgtcccc agaataatgg gggaccggat cacctcgcaa acgaggacct gatgtagcat
     3241 ggatgctccg gcgacttctg ttctcgagta tgccttgtgg cctttgttga ccttcacgat
     3301 agtagccctc tggcttgcag cagtggtgga tatctcacgc cacaggcgta gaggaacgtt
     3361 cacgggcgca gggttgctgg gcatggctat gctcgtctcg atcgtcgcgg ttatcgcgtg
     3421 cgctggtacc agcgcggttc ttgctacccg gtgggtcgaa gccgtgtcaa gagcaggttt
     3481 cgctgggatc gaagccggca agctggagga tgacggttcg gcacgtgcct acattttcag
     3541 cttcggtctg gagaatactt cttatggatc atcggtcaat tcgacaggca cgaaaaagag
     3601 ccagaaccac gcaccccgtt aaattcttgc agttctggag cgagttggac aatgttgtgc
     3661 ttgcctcatc tgagttctct ctgctgagct ctctgctaag aacggccgcg ctcggtaccg
     3721 atctttcgcg gcacttggat ccgtgaacgt cccccgccca tcactggcgc cgggccaggt
     3781 atatacccac gagtatgacc gccccgccta cgagcttctc gatcccgagc acctcgctaa
     3841 agaagacgat gcctgaggca acccccgtga tggtgatcaa atattggtag accagaactc
     3901 tgttggcccc gatgcgggat acgcccgttt gccaggccga gaaggagaaa gcggtggcga
     3961 agaccgccga gaagcccaca gccgcccatg ctccgatgcc gacgctcccc cactccaaac
     4021 cggtaaggta cggtgaagag agcagcagga cgaccagccc tccgaagagc aaggagtagg
     4081 tcgccaccgc cagcggcgag tgacgctcca gcaccggcat cgagagtacg gtgtagctcc
     4141 cgaacgccat cgccgccacg agcacgaaga gatctccggc caggctcgta ccctcggtcc
     4201 cgaggccctc gtagaagacg atgccgacac ccaggatcga aagccccacg cccaccatgc
     4261 ccctccgcgt gggcctctcc aacccgagca tcaggctcag gaacagtccc cagatggggg
     4321 cagtggcgaa gatgagaccg gtgttggcag cactggtcat gctcacgccg aaggtgaaag
     4381 ctgtctggcc gatcgccacc ccgaggcacc ccaggcccgc cacggagaga aggtctttcg
     4441 gtttgagcct gtccgctggc tccaggatgc ggaggaggca gaacatcaag ctaccgcctg
     4501 ctgtgaagcg taaggccacg atgagcagtg gaggcatgaa ctcggccgcg tacttcgtcg
     4561 ccgcgtagtt ggcgccccag aagacggcgg ctagcatcaa agagacctcc gtaaagatgg
     4621 ccgccttctt taggtctgtc acgagacgag attataagcc cggaccgcat cgagcgcacc
     4681 gaggccggaa gtgcgcgagt gaagggaact aggataatta tttgactacc gctggtcgat
     4741 cacaagaagc tatctatgct tcatgcgacg ggagcaccgt tccaatgcgc aacattgtag
     4801 acggttcggt tgcagcgcag tggcatggcc ggggagtagg atccagatcc ttcatccgct
     4861 gcttgcgggc attttcttag ctgccggaga aggattcgaa ccttcactaa ctgatccaga
     4921 gggagatgtt gaaccgcaaa aggtgcaggc tgtgcctcct cgctctaaat cctcaacaac
     4981 actactctgc gactgctacc ctcgtccagc gaggcgtgga accgtaaccg aaggcggtta
     5041 gtagatgtgg accaagatag agacatcgtt ttcccctaag ctctgacaaa ttccacttgc
     5101 tgggtcccgt cttctgtgag catcgcacga aacctgagct cccttgtgtt tgtggtttgg
     5161 caacaatacg gtgatcgtcc tcacaacggc ggtcgcctcg gggcgtcgaa atggggtgat
     5221 gtctgatagt gtttgagagg atcccgga
//
LOCUS       DASTKM010000361         4183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_167593, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000361 DASTKM010000000
VERSION     DASTKM010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167593"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFQ10_14285"
     CDS             complement(<1..266)
                     /locus_tag="VFQ10_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966558.1"
                     /translation="MGTEPGATAARSAKVNSGKRRGEEPPITNDALIEEINLAYRRLK
                     AATEDLVSADRELAKHIKRVRLDNAEAILEAKNERTATLYLDGLL"
     gene            complement(342..593)
                     /locus_tag="VFQ10_14290"
     CDS             complement(342..593)
                     /locus_tag="VFQ10_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966559.1"
                     /translation="MESTAGMLVLARDMRAHNHYVREATSLEPHMNRAWAYGLQRQAE
                     FGYEGFFRLYRRYAQTARMVQLSEESIVAGCNEVLEGLH"
     gene            complement(847..1506)
                     /locus_tag="VFQ10_14295"
     CDS             complement(847..1506)
                     /locus_tag="VFQ10_14295"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9966560.1"
                     /translation="MDAWVDEWEIYPGDSLVQKIFEEGLMDAEAVIIVLSEHSVDKPW
                     VREELDASIIKKVEDLITLIPVVIDECEVPESLRSTVWQKIENLEEYGTEFDRIVAAI
                     YGHRGDKLELGPPPEYSQTAVDTLPGLTSVDTLVLKLSCQKAIEGFDYFIQAHDVWEQ
                     VDAVGVPLVEFNDSLEVLHGRGYIRTDDETLGPADESPLSYTIQIRGFEEYARAFVEN
                     Y"
     gene            complement(1787..3052)
                     /locus_tag="VFQ10_14300"
     CDS             complement(1787..3052)
                     /locus_tag="VFQ10_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like protein"
                     /protein_id="HET9966561.1"
                     /translation="MTEAPIVVENREELIFLLSEASELEHMVMLAYLFAAFSLKTDAG
                     EGLTEEQLEAVRRWERVISEVAAQEMLHLALASNLLTAIGGAPHFSRPNFPQPAKYYP
                     PGFELALMPFSEQALGSFVFYERPEGMEQDAPELELASEVIAPLIPGGVVVPHEQQFA
                     TVGHLYRGIEQGFRYLVEKYGEHRVFVGPPEAQATWSYFWWPELVPVTDLASAQMAIE
                     SIVEQGEGARSDRDDAHFGMFLRVLVEYLEYKKRDPDFQPARPVTPACVRPPGDAKLS
                     NPTLITDPTTAGVADLFNATYEVMLQMLVRFFGHTEETEEELQTLSSTAVDAMSLLIK
                     PLGQLLTTLPVGAEYPGKSAGPSFEFYRTSYLLPHHHAAWVLMNERLLELAVYCARLG
                     ARPSAPPELTTIEGTFRKLARALERHIGR"
     gene            complement(3118..3618)
                     /locus_tag="VFQ10_14305"
     CDS             complement(3118..3618)
                     /locus_tag="VFQ10_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9966562.1"
                     /translation="MVKDEVKATGAVVTGRRTFELADDWGGDHHDGHPIFALTRREPG
                     SETEQWPLITYATDVRTAMTEAKQAAGDKNVLVHGAGTAQLALAAGVLDELEIHLMPV
                     LLGQGRSLLLFALRTNRQLPPFRPFFALRVSFRRLFILKSGWVTPTHARVSTTGLRSI
                     EVDGYR"
     gene            complement(3942..>4183)
                     /locus_tag="VFQ10_14310"
     CDS             complement(3942..>4183)
                     /locus_tag="VFQ10_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966563.1"
                     /translation="NDLSMQYFDSRGVHRVYAIGFDGRELTLGRDAPGFAQRGSAKLS
                     DDGSTLAGVWQLNEDDQGYRDDLAFTYRRASGGDR"
BASE COUNT          741 a         1344 c         1215 g          883 t
ORIGIN      
        1 agcaggccgt cgagatagag ggtcgctgtc ctctcgttct tagcctctag gatagcctcg
       61 gcgttatcca ggcggaccct cttgatgtgc ttggccagtt cgcggtcagc gcttaccaga
      121 tcctccgtgg ctgccttcag ccgacggtac gccaagttaa tctcctcgat gagcgcgtcg
      181 ttggttatcg gcggctcctc tcctcgcctc ttcccgctgt tcaccttcgc cgaccttgcg
      241 gcggtcgccc ccggctctgt ccccatgctc tcctgagctc tgcccgcgcc tctcgtctag
      301 tctaacgtca gccggaatat cgtcccggct cgcgcgactc ctcagtggag gccttctagc
      361 acctcgttgc agcccgcgac gatgctctcc tccgaaagct gtaccatcct tgccgtctgt
      421 gcgtagcgcc gatacagcct gaagaacccc tcgtatccaa actcggcctg gcgctgaagc
      481 ccgtaagccc acgccctgtt catgtggggt tccagagacg tggcctccct cacgtagtgg
      541 ttgtgtgccc tcatatctcg cgccaggacg agcatcccag ccgtcgactc cacgctgaac
      601 aggccactgc gggcggcctc cgcgatcacg agcgggtgtg ggtggtggat gctgaggcgt
      661 atctcgtggg agacagaagg acgcagggat ctcgataaat cccttgcgct ccagcagctc
      721 gaggatgtga ttaacgatca tcttggggtg tctaagttcc tcagttatgc cctcgctgtt
      781 cagcctaccc gcttgcgctt cctgcgcatg attgacgacg tgaaaggcca ccgcatcgat
      841 gatgctctaa tagttctcaa cgaaggctcg agcatactct tcaaacccgc gaatctggat
      901 ggtgtagctc agtggactct catcggccgg gccgagcgtc tcgtcatccg tcctgatgta
      961 gccccgtccg tgaagcacct ccaagctgtc gttgaactcc actaagggca cgccgacagc
     1021 gtccacttgc tcccacacat cgtgggcctg tatgaagtaa tcgaagcctt cgatagcctt
     1081 ttggcaggag agtttcagca ccagggtgtc cactgaggta agaccgggga gcgtatcgac
     1141 agcggtctgg ctgtactcgg gcggcggccc aagttccagc ttgtctcctc tatgaccgta
     1201 aatggccgct actatacgat cgaactctgt gccgtactct tcgaggttct cgatcttctg
     1261 ccatacggtc gatcgcagac tttccggaac ctcacattcg tctatcacga ctgggatcag
     1321 cgtaatcagg tcctccacct tctttatgat gctggcgtcc agttcctccc gaacccaagg
     1381 cttatcgaca ctatgctcag agagtacgat gattacagct tctgcgtcca tgagaccctc
     1441 ttcgaatatt ttctgtacga ggctgtcgcc aggataaatc tcccattcat ctacccaggc
     1501 gtccaccccc gcttcgtggc gaaagccctg agaaagcgct ccttatcctc gctcgcatgg
     1561 ctgatgaaga ccttcggatt gatgttactc ctcctcataa tcgccgcaat ccgacgtctc
     1621 gagcggtgca ccagtctcta accctagcag gttgtaacgg ctgcggggac ttactacatt
     1681 cgtacggacc atccgccacc gccatagcgt cgcctcgcag ctcgctttct ttcagaacag
     1741 aggtaaaaag gcgctggtgg atacacttgc accagcgccc gccgttttat ctgccgatgt
     1801 gtcgctccag cgcacgggcg agcttccgaa aggtcccttc gatcgtcgtc agctctggtg
     1861 gagcggacgg ccgggccccc agcctggcgc agtaaaccgc caactcgagg agccgctcgt
     1921 tcatgagcac ccacgcggcg tgatggtgcg gcagcaggta gctcgtgcgg tagaactcga
     1981 aactcggccc cgcactcttg cccggatact ccgctcccac cggcagggta gtgagaagtt
     2041 gtcccaaagg cttgatgagg agggacatcg cgtctacggc ggtactgctc aacgtctgta
     2101 gttcctcttc ggtctcctcg gtgtggccga agaagcggac cagcatctgc agcatcacct
     2161 cgtaggtggc gttgaacagg tcagctaccc cggccgtggt ggggtcggtt atcaaggtgg
     2221 ggttggacag cttcgcgtcg cccggcggcc tcacacatgc cggcgtaact ggcctcgccg
     2281 gctggaagtc cggatcccgc ttcttatatt cgaggtactc caccaggacc cggagaaaca
     2341 tgccgaagtg ggcatcgtct ctgtcgctcc gggccccctc gccctgctcg acgatcgact
     2401 cgatcgccat ctgtgccgaa gcgaggtcgg tgaccgggac caactcgggc caccagaagt
     2461 aactccaggt ggcctgggct tcgggcggtc cgacgaagac tctgtgctct ccgtacttct
     2521 cgaccaggta cctgaagcct tgctcgattc cccggtagag gtggcccacg gtggcgaact
     2581 gctgctcgtg aggcacaacg acgccgcctg ggatgagggg cgctatcact tcgctcgcga
     2641 gctccagctc cggcgcgtct tgctccatgc cctcgggtcg ctcgtagaag acgaagctcc
     2701 ccagggcctg ctcgctgaaa ggcatgagag ccagctcgaa tcccggtgga tagtattttg
     2761 ccggctgcgg gaagttcggc cgactgaagt gtggggcacc gcctatcgcc gtgagcaggt
     2821 tgctcgccaa ggccagatgc agcatctcct gggcggcgac ctccgatatc acccgctccc
     2881 accgtctgac cgcctcgagc tgctcctcgg tcagcccttc gccggcgtcg gttttcagac
     2941 tgaaggcggc gaaaaggtac gccagcatta ccatgtgctc tagctctgag gcctcggaga
     3001 gcagaaagat gagctcttcc cggttctcga cgacgatcgg ggcttccgtc aacggtctcc
     3061 tccttccctt acgtggagtg tagatcgtac tttatgcgaa gaatcttgtg catgccctta
     3121 ccgataaccg tctacctcga tcgagcgcag gcccgtcgtc gacacccgtg cgtgtgtggg
     3181 agttacccaa ccactcttca aaatgaagag ccgacgaaag ctcacgcgca gagcgaaaaa
     3241 aggccgaaag gggggcagct gcctgttcgt ccttaaggcg aacaacaaga gactgcgccc
     3301 ttggccgagg agcaccggca tgagatgtat ctcgagctcg tcgaggacgc cggcagcgag
     3361 cgcgagctgc gcggttccgg cgccgtggac cagaacgttc ttgtcgcccg cagcctgctt
     3421 ggcctccgtc atcgcggtcc tgacgtcggt cgcgtaggta atcagcggcc actgctccgt
     3481 ctcgctgccg ggctcacgcc gggtgagggc gaagatcggg tggccgtcgt ggtggtcgcc
     3541 gccccagtcg tccgcgagct cgaaggtccg ccggccggtg acaaccgccc cggtggcctt
     3601 cacttcgtct ttgaccatgc cgttgacgcc cgccaaacga ccggagagtt ggttggcgtc
     3661 ggcacctctc cagcgcggat ctcccgaccg gcatcacgtc gagtagatca cgaccaacga
     3721 ctatggtgtc tgcaacggac atgagcatgc ccgtcagttc gtcctcgagc cggggggcat
     3781 cgaccataaa gtcgaggtcg ttgtccggtc cggccatgaa tccatcgagg gacatggaca
     3841 tgtagagaac agttgcggac acggcgtctc ctttcctggt ctgtggtcta gctcggatct
     3901 ccctgcctcg acggggagac tcattcctcg gtcataccgc gctagcggtc gcccccggaa
     3961 gcccgccggt aggtgaacgc gaggtcgtca cggtatcctt ggtcgtcctc gttcagttgc
     4021 cagacgccgg cgagggtcga gccgtcgtcg ctgagctttg cgctgccgcg ctgagcgaac
     4081 cccggggcgt cgcgtcccag tgtcagctct ctgccgtcga acccgatcgc gtagacccga
     4141 tggacgccgc gcgagtcgaa gtactgcatc gagaggtcgt tct
//
LOCUS       DASTKM010000362         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_1581064, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000362 DASTKM010000000
VERSION     DASTKM010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581064"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VFQ10_14315"
     CDS             complement(<1..959)
                     /locus_tag="VFQ10_14315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9966564.1"
                     /translation="MTPRLTVVIPNWNGKRFLKLCLGSLRDQSFKDFETVLVDNGSTD
                     GSRAFAEKHFPEVNVVPLGENLGTAAAFNAGIEASRTEYVVLLNNDTEQDPGWLEALV
                     RAAADHPESGLFASKLVDFYDRRVLDGAGDAMRLSGLPYRLGHGERDSGRFDKPGYVF
                     GACAAAALYRRDMLDEVGLFDEDFVSYCEDGDLSFRAQLAGYRCFYVPGAVVYHMGSA
                     STGGKRSPTATRLGSRNSFSLLVKNLPISAVPHVLPFFVLGQFVRLITAAATGSLRAH
                     LEGLAGAWRHLPLMLGKRAEIQKRKKLSDAEVRRLLRESSLAAT"
     gene            complement(964..2322)
                     /locus_tag="VFQ10_14320"
     CDS             complement(964..2322)
                     /locus_tag="VFQ10_14320"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9966565.1"
                     /translation="MRPAISLRGVSKKYIISPSRSSRLGELLSFGKAKRSREFWALQD
                     IDLDVEPGTTLGVVGRNGAGKSTLLSIISGVLRPTTGEVQVEGRLSAIFGLGAGFNPL
                     FTGRENAMLSGLILGIEYEEMIERFAAIEAFADIGEFMDQPIKTYSSGMRSRLGFAVA
                     INVEPDVLVIDEALSAGDSAFKKKAIQRMYDLRDSGSTVLFVSHSMGMVKRFCTQAIL
                     MHKGNLVSTGSPEEVVDYYEEMLAKSQEMKDAAETSELDEQLDEQLDYEIEHEEEEDT
                     PTKGEGFGGRRPQQTRRVVGGAEILGLELLDENGQPAGTLPSGATLSVRVRVRYEEAM
                     EESTLGISLQSHRAGVEVFWTDTAREEAPIGRKEAGEQVKVEFTFEVPLGAGSYNVEA
                     SLTDGRPEGSDVARARKARTFEITQQGSLGPVRGLVRVPTKVEISSALEAEEQEGPVR
                     PA"
     gene            complement(2319..>2600)
                     /locus_tag="VFQ10_14325"
     CDS             complement(2319..>2600)
                     /locus_tag="VFQ10_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966566.1"
                     /translation="KRWLLRYFVRRQVTRSYKRSYLGLAWAILGPLIWVFFLTIIFSE
                     VVGAYRGMILESTLPGGLATLYFSVFSIALFFVGFALFVRFKAGFADHI"
BASE COUNT          482 a          848 c          768 g          502 t
ORIGIN      
        1 gtcgcggcga gtgatgactc tctgaggagc ctgcggactt cggcgtcgga gagcttcttc
       61 ctcttctgta tctcggcccg cttcccgagc atgagcggca ggtggcgcca cgcgcccgca
      121 agtccctcca ggtgcgcccg caaagagccc gtcgccgcgg cggtgatcag gcgcacgaac
      181 tgtccgagga cgaagaaagg caaaacgtgc ggtaccgcgg agatgggaag gttcttgacc
      241 agcagactga aagagttgcg gctacctagc ctggtcgccg tcgggctacg cttgccaccg
      301 gtcgaggcgc ttcccatgtg gtagaccacg gcgccgggga cgtagaagca acgatagccg
      361 gcgagctggg cgcgaaagct caggtcccca tcctcacaat aggagacgaa gtcctcgtcg
      421 aagagcccga cctcatcgag catgtccctg cgatagagcg ccgccgctgc acacgccccg
      481 aagacgtagc ccggtttatc gaacctgcca ctgtcgcgct cgccgtgtcc gaggcggtaa
      541 ggtagaccgc taaggcgcat ggcgtccccg gcgccgtcga gcaccctgcg gtcgtagaag
      601 tcgacaagct tactcgcgaa gaggcccgac tcggggtgat cggccgccgc gcgcaccagc
      661 gcctcgagcc agcctgggtc ctgctcggta tcgttgttga ggagtaccac gtactccgtc
      721 ctagacgcct cgatcccggc gttgaacgcc gcggcggtgc ccaggttttc gccgagcggg
      781 accacgttta cctcggggaa gtgtttctct gcaaaggctc tcgagccgtc tgtggagccg
      841 ttgtccacca gaacggtctc gaagtctttg aaagactggt ctcgcaaaga gcccaggcaa
      901 agcttcagga accgcttacc gttccagtta gggataacga ccgtgaggcg gggagtcacg
      961 acttcaggct ggccggacag gcccctcctg ctcctcggcc tccagagcgc tgctgatctc
     1021 gactttcgtg ggcactcgca ccagacctct gactggtccg agtgaccctt gctgggttat
     1081 ctcgaaggtc ctcgccttcc gtgcgcgggc cacatccgag ccctcaggtc gtccatccgt
     1141 aagggacgcc tccacgttgt aggagccggc tccgagcggc acctcaaacg tgaactctac
     1201 cttcacctgc tcgcccgcct ccttccgacc gatgggagcc tcctcccgcg ccgtatccgt
     1261 ccagaagacc tcaacaccgg ctctgtggct ctggaggctg atgccgaggg tgctctcctc
     1321 catggcctcc tcgtagcgca cgcgcacccg cacgctcagg gtcgcgcccg agggcagcgt
     1381 gccggccggt tgcccgttct cgtcgagcag ttcgaggcca aggatctcgg cccctcccac
     1441 gaccctgcgg gtctgttgtg gcctccggcc accaaaacct tcgcccttgg tcggagtatc
     1501 ctcctcctct tcgtgctcta tctcgtagtc gagttgctcg tcgagttgct cgtcgagctc
     1561 gctggtctct gcagcatcct tcatctcctg ggacttggcg agcatctcct cgtagtagtc
     1621 tacaacctcc tcggggctgc ctgtgctcac aaggtttccc ttgtgcatca gtatggcctg
     1681 agtgcagaag cgcttgacca tccccatgga gtgggagacg aaaaggaccg ttgatcccga
     1741 gtctctgagg tcgtacatcc tctggatcgc cttcttcttg aatgccgagt cgcccgccga
     1801 gagggcttcg tctatgacaa ggacatcggg ctcgacgttt atggcgacgg cgaagcccag
     1861 gcgactgcgc atcccgcttg agtaggtctt gatcggctgg tccatgaact ccccgatgtc
     1921 cgcaaaagcc tcgatggcgg cgaagcgctc gatcatctcc tcgtattcta tgccaaggat
     1981 cagtccgcta agcatggcgt tctcgcgtcc cgtaaagagc gggttgaagc cagcccccaa
     2041 cccgaagatc gccgagagcc tcccctcgac ctggacctcc cctgttgtgg gcctgagaac
     2101 gccagagatt atgctaagaa gcgtgctctt gccagccccg ttgcggccca ccaccccaag
     2161 ggtggtgcca ggctctacgt ccaggtctat gtcctgcagc gcccagaact cgcggcttcg
     2221 cttggccttg ccgaagctca gtagttcgcc aagcctggag gagcggctag gggagatgat
     2281 gtacttcttg gatactcccc tcagggagat ggccggcctc atatgtggtc agcgaaccct
     2341 gccttgaacc tgacgaacag cgcgaagccg acgaagaaca aggctatgga gaacacggag
     2401 aagtagaggg tggcaagccc gcctggaagc gtactctcaa ggatcatccc cctgtatgca
     2461 ccgaccacct cagagaagat gatcgtcagg aagaagaccc agatcagagg tcccagtatc
     2521 gcccacgcaa gacccagata ggatctcttg tagctgcggg ttacctgacg gcgtacaaag
     2581 tacctcagca accaccgttt
//
LOCUS       DASTKM010000363        14226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacter sp. isolate SMAG_U2078 k141_525247, whole
            genome shotgun sequence.
ACCESSION   DASTKM010000363 DASTKM010000000
VERSION     DASTKM010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970008
            Sequence Read Archive: SRR25158498
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacter sp. (soil metagenome)
  ORGANISM  Rubrobacter sp.
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:04:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,878
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,833
            CDSs (with protein)               :: 2,833
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 4 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /organism="Rubrobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525247"
                     /isolate="SMAG_U2078"
                     /isolation_source="soil"
                     /db_xref="taxon:2036010"
                     /environmental_sample
                     /country="China"
                     /lat_lon="47.35222222 N 87.843463 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFQ10_14330"
     CDS             complement(<1..249)
                     /locus_tag="VFQ10_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966567.1"
                     /translation="MSSTEDRARMGAMPNAEAAASRTFAHEPGEGEAWWLGMLATIKA
                     SSEQTGGKYSVVEILAPDGYGSVVHVHHREDEGFYILEG"
     gene            371..946
                     /locus_tag="VFQ10_14335"
     CDS             371..946
                     /locus_tag="VFQ10_14335"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9966568.1"
                     /translation="MKRRAERMRETRRRITEATVELHQAVGPARTTVSAIAQKAGVQR
                     HTYYAHFPETKDLYQACTAHYLERNPLPEPSRWEEIPGSEERLRVALSEVYRYYSGNE
                     AMLTNVLRDMPFDPVLQENNVLLFRHWEAMRETIADAFEASGERRETLLGAIALALEL
                     QTWRTLVRQQGLEDDRAVELMVGMVRCLMRS"
     gene            complement(1566..3575)
                     /locus_tag="VFQ10_14340"
     CDS             complement(1566..3575)
                     /locus_tag="VFQ10_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966569.1"
                     /translation="MGTNLYYNRGDLSDEGLNISAPLDDTFIHLQYGRQIGEGQWFSY
                     NDGDPVSSGASSFLYVLLLGAAHFVGFSGANLLGFAIIFGTVLFVLSALLGYGLGQRL
                     AGERAGLCSGILIAANGAFGWGATSGMEVALFSVLILGTLLAFLRELSSGRFLLTPIL
                     AALAALTRPEGLLFAWVITGAVVFVLLRDLRKSRPYSLRSSLAVLYAFLPIAAGVAQY
                     MFYTIATGSSIQNGVLAKSLLYEPVFYPTEVLDAVFQNLTKLSLIVLTGLEPGNYLFP
                     GTILFCVLGTMYLALEDARYRMFAVASGTALVLAMSSTAILGLPGAWGWHHYRYLLPF
                     FPPVLVFAVVGFYSLRSLTRKTWLPEALASFAILCSLLGLPVWAATTGGNSLQIKEQQ
                     VSVGYWIRENLPPGTSVGVNDAGAMRYYGGHRTVDLIGLTTNDLALPTRNGVGSLYET
                     LEKMPEEKRPDYFAIYPTWFPGFEASGVLDQEIARFAISDRPEVAGIVGGSEVVVFRA
                     DWSLAQSGEAFQGEGSVKDTLDIADLGSETEHDYEMHMPLIGLEPANFLTRERSPDGE
                     MVVDGGRGLPGAEEFTIRGLSRNRPVEVVMRTTSAPFALQVQADGRYVGEWDFQPSGS
                     RWQQATFTIPAESVRSGSLRVRLSPPEDAPLETHTAYYYWFVQRI"
     gene            complement(4203..4598)
                     /locus_tag="VFQ10_14345"
     CDS             complement(4203..4598)
                     /locus_tag="VFQ10_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966570.1"
                     /translation="MRTKIAMMIAMTVVLVALMVGSAGTAFAQMIGGVIQCDSVPCVA
                     TGDHQVLFEQVGDGVPDRMIAQGGHDHLDARAYTNDRDVAKGSGGHDLLRVNDGDAMD
                     GAIGGPGSDLCVVDAAIEAADTCEGVIYR"
     gene            5463..6077
                     /locus_tag="VFQ10_14350"
     CDS             5463..6077
                     /locus_tag="VFQ10_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET9966571.1"
                     /translation="MADSTSQAEQGFFSRIASDFTTRAWVLIPVGVGVNFVGGTLVTV
                     LKLPIFLDTIGTILVALLAGPWVAALTGLITNLVLVVSDPTLIAFAPVNVAIGLIAGF
                     LALRGWFKTYPKIVLSGLIIMLTGAILSAPIVVLAFAGVQGSPGRSALTAFFLATGSN
                     IWQSVFTTSVIVEVVDKLISVFVAVFVARAIPERYRPQKAQQTL"
     gene            6101..6862
                     /locus_tag="VFQ10_14355"
     CDS             6101..6862
                     /locus_tag="VFQ10_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET9966572.1"
                     /translation="MFDYRPGDSPVHLLNPVTKLAVAVGLTVVVFLLPNPWGPALIAV
                     ALLGVAAVAGFPGRVAKLAAILTLPFLVAVVLVQGIFYPGNETPLLEVGPVTFWEEGV
                     TYGLLVFFRVLVVVCAVLIMVFSTYPKAMMVAFIDKGLSPKLAYVFMASLQFVPEMQR
                     RARAIIEAQQARGLDLKANLWRRFQALLAMMIPLLAGALISVETRSLALEARGFSRGG
                     MRNHLIEVPDRTTDRVIRWSMAAIVVLVATWRIVL"
     gene            6859..8571
                     /locus_tag="VFQ10_14360"
     CDS             6859..8571
                     /locus_tag="VFQ10_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET9966573.1"
                     /translation="MSLIEIEELTFSYSTQQHEPALRNLSCSIEQGSFVGITGLAEAG
                     KSTFCRLIAGYIPHFFQGEFSGRVNVAGKDTTETTIGELAEWVGFVFENPFDQLTGAS
                     LTVLEEAAFALENMGLAREQIRLRAEKSLTQVGMEDLKDRHPQQLSGGQSQRLALASI
                     IAVQPEVFILDEPTSQLDPLGVEEVFDVISDMHTRGNTLIVVSQDLDHLAIRSDRLMV
                     IDKGEIKWDGEPREVLLEAAEARYPILIPDVLEISRKLRAAGRIPSNPPLPLTVEQAA
                     NELSSIDTPGSAETIAAGRTSRHSKVESSKELVFEDVHYDYPTGVSAIRGVSLSLDEG
                     CVCIVGQNGAGKTTFAKHLNGLLRPTRGRVLVRGKDTREYRVAELAREVGLAFQNPDD
                     QLFRSTVEEEVRFGPDNVGAGPEEAKRMVAFAMDLMGLEAVPEKKPHDLGVPERKRVA
                     TASVIAMNTPVVVLDEPTGGQDAEGIELLGQLVGQLVQQGKLVVVVTHDVMFAARHAD
                     RVIALYQGRVLLDDDPRTVFGREETLARTHVEPPAVTRLGKLLGLEETLLSPEELLAV
                     FAPE"
     gene            complement(8603..8734)
                     /locus_tag="VFQ10_14365"
     CDS             complement(8603..8734)
                     /locus_tag="VFQ10_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9966574.1"
                     /translation="MTLAYLRECSRLDILREYILRIFALLPHPCMLHQRRLLFLLSS"
     gene            complement(8835..9218)
                     /locus_tag="VFQ10_14370"
     CDS             complement(8835..9218)
                     /locus_tag="VFQ10_14370"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9966575.1"
                     /translation="MDLETNSAGKQEILASGVHGRMLVARTWFGRTRKEVADEYLRYN
                     YENGVLEVEKKPGCLGMQQFRKMHGDVAEFTTISYWSSMEAMEAAHSGDLRPSHLEKD
                     PDYLLELPESVEVSELHVNDWQLST"
     gene            9314..10099
                     /gene="budA"
                     /locus_tag="VFQ10_14375"
     CDS             9314..10099
                     /gene="budA"
                     /locus_tag="VFQ10_14375"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET9966576.1"
                     /translation="MPIDTALVGALHVEYLRERDFSGDEHLHHTLFQTSTIDALLEGK
                     YEGDVSFAELEGNGDFGLGTFDALDGEMICLDGTFYQVRADGRAYAVDGKARTPFAVV
                     TLFEPDVSLTLSIPMGFEGLCDYVDGVIGGGAVCAAIRVDGSFECVKTRSVPRQRKPY
                     PPLVEVVKDQPTFELHDVSGSLVGFRFPDYAQGLNVSGYHFHFITADRSAGGHVLGCK
                     LVRGELYIDHEGDLRLELPPDVGLPTSDQSAAKGETLDRIERE"
     gene            10228..12366
                     /locus_tag="VFQ10_14380"
     CDS             10228..12366
                     /locus_tag="VFQ10_14380"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET9966577.1"
                     /translation="MRSTSSRTLEDWEALPIAAPLGSAREAAGIRRAWTPIRVPWHWQ
                     LEDAFATYEGIVLYRCRFAQQRPTEGTMLSLRFEGVYYAARVWLNGVYLGEHEGYFSA
                     FEFDITDVVVSAGENELLVEVCSPEESDENDRKTVGGVWARWDGMAPHINPGGIFREV
                     TVVVDGEVRIRSLGAAADHSGRGVAHVDLYSRYNARARLVGRVRPLGFSAPDVEFERE
                     VQVEGGENRLEIGFRVPDVRVWSTWDRGEQPLYELVLWCAGAEERTRFGASTVELRDW
                     KVYLNGERIFLRGINYLPSDAYPARATEELLRADASLVREAGMNAVRVHAHVSDREFY
                     EACDELGLLVVQDFPLQWTHRRSILGPAVTQAGEMARILRGNASVGIYLAHDEPFFVA
                     PPEKWSPLGLLRTAAEVLSPRWMLWQRRVLDPAVVRAISEEDDSRPVIDAAGHPLTTN
                     HLYFGWYYGKFRDLERVVKVFPGFPRLPTEYGAQALPDPESLEEIWPSGTRPDWSVLS
                     LNYRLQVQRITRYVPWRGDRATFLRESQRYQAEVLKHATELFRRRKYRPTGGTFAFML
                     NDPAPAVSWSVVDWRRRPKIAYETLLDAMRPVLICAEYPKERYSVGDRISLPLFVIND
                     LSRELGIVDWSWELFLGGSSAAHGGGETTVPKDSVVRIGEAVATLSTSGPAVLRLTLF
                     GEEGPLSNEYEFVVSQAGARAHRGPSARGG"
     gene            complement(12314..13144)
                     /locus_tag="VFQ10_14385"
     CDS             complement(12314..13144)
                     /locus_tag="VFQ10_14385"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9966578.1"
                     /translation="MRHPCAWTVVATTAVCGAALAGLRTATGRPVRSVPTLAAAGASM
                     AAGGFMAYEWLTPRSWLYGPVFWHARTNERVVALTFDDGPCHPYTGHLLEILNREGIR
                     ATFFQVGKNVMREPNLAAEVASQHEVGNHTFTHPHLAWSRPRMVREEMLRGQEAIHGA
                     TGTLPQLFRVPHGRYGPQVISVAEELGMRCVGWSVMAWDWYRPPKDMIQRRILHGVGP
                     GGVTLLHDGQDTDAFPKADRSHTVAAVSDIISGLKASGYGFLTLPELMDLDAREHQPG
                     "
     gene            complement(13141..>14226)
                     /locus_tag="VFQ10_14390"
     CDS             complement(13141..>14226)
                     /locus_tag="VFQ10_14390"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9966579.1"
                     /translation="IGSGHMRASAAVVRGVDILDPERTCSIVDFPHEVSPGVERLLRD
                     AYLEALKLWPTAYGKLYESSGTGQPGSYVNYLRSAGLKTLERLVSSTDAKALVAPHFY
                     GAGVLGSYKEQNPGMFSAVVLTDYVPHPIGVPLNLDLYVVADDVAAEDVAKLGVSEEQ
                     IHPTGIPIDPIFEAPADPGPARREILNLDDDDLPVVLVMGGGLGGGDLEESVSLLLQA
                     SEAMHLVVLCGSNERSRTRLERLAERGGHKATFLAFTDRVRELMAAGAVLVTKPGGMS
                     CTEALASRLPQVLYHPIPGQEEDNAAAIVRYGAGVMVRDTSQLLGQALKILTSADHRH
                     NMVQAAEAAHRPHSARSAASLVLAGLP"
BASE COUNT         2767 a         4289 c         4367 g         2803 t
ORIGIN      
        1 gccttccagg atgtagaaac cttcgtcctc tcggtgatgg acgtgcacca cgctcccgta
       61 gccatcagga gcgagtatct ctaccacaga gtacttcccg ccagtctgct cagatgaagc
      121 ctttatggtg gccagcatcc ctaaccacca cgcctctcct tcgcccggct cgtgcgcgaa
      181 agtcctgctt gcggctgctt cagcgttggg catggccccc atcctggctc ggtcttctgt
      241 agaggacata gtgtctttct cctttcccta cacactaatg tgcagtgaac gatgccagag
      301 aacgaaccgg ctgtcaaggt taaatgcaca aatgtgtgca ataatttctt ccatgccacg
      361 aaaatacgag atgaagcgca gagccgagcg catgcgggag acccgccgaa ggatcaccga
      421 agccaccgtg gagctgcacc aggcggtggg tccggcgcgg accaccgtga gtgccatcgc
      481 ccagaaggcg ggcgtacaga ggcacactta ctacgcccac ttccccgaga caaaggacct
      541 ctaccaggcc tgcacggccc actacctgga gcgaaacccc ctgcctgagc catcccgttg
      601 ggaggagatc cccggctccg aggagcgcct gagggtggcg ctctctgagg tgtaccgcta
      661 ctactcaggc aacgaggcga tgttgaccaa cgtgctgcgt gacatgccct tcgatcccgt
      721 cctacaagag aacaatgtct tgcttttccg ccactgggag gctatgaggg agaccatcgc
      781 cgatgcgttt gaggcaagcg gcgagcggcg cgagacgctg ctgggggcta tagccctggc
      841 cctggagttg cagacgtggc gcacgctggt gcgccagcag ggcttagagg acgaccgggc
      901 ggtggagttg atggtaggga tggtgcgctg cctgatgcgc tcctagtgtt gtttttccaa
      961 cttctgagaa cctcgtcatg gcgaagtagt tcgcagaatc tactcttcat cctttcgtcc
     1021 tccgcccgct aaagaaggag acagggatga gaaggacccc tttgttgttt tcgacggcgg
     1081 tagttcttct tgccagcggg gctgtactgg cgttgccgtc ctaggtcccc gacgacaccc
     1141 cgagggtcga tggacgcgtc agggctatcg agcacctagg caccaaaatc tggctgacat
     1201 gacactctat ggcgacgcct tgggccttgg cggcggctct gacaaatgcc gcgggggtgt
     1261 ggcgaggact atattcccat cgactctctc atcctgttct cagaatcgcc ctccatactg
     1321 cgccttttcg gggctcggcg attgtcccaa caatatcatc ctttctttcc cgagccctga
     1381 agaggccagt acccttatat acgagggcat ccctcaggtt ggactgttat tgatacattg
     1441 cgctgagtgc gaacaccaaa gcagcctcgt gctctctata ccctttgggc cgcacagttc
     1501 ttaccgagtt ggtcggagag cccgagactc tcctggtcga ctcgacattc ttgggaacgc
     1561 ttagatcaga tccgctggac aaaccaatag tagtaagctg tgtgtgtctc tagcggggcg
     1621 tcttctggtg gtgagagccg cactcgaagg cttccagagc gaacagattc ggcaggtatc
     1681 gtaaaggtgg cctgctgcca cctactaccg gagggctgga aatcccactc cccaacgtat
     1741 ctgccatcgg cttgcacctg cagggcaaaa ggcgcactgg tggtgcgcat caccacctcg
     1801 accgggcggt tgcgggagag acctctgatg gtaaactcct ctgctccggg cagcccgcgc
     1861 ccgccatcga cgaccatctc accgtcgggg gagcgctccc gcgtgaggaa gttggccggt
     1921 tcgagcccga tcaggggcat gtgcatctcg tagtcgtgct ctgtctcgct tcctagatcg
     1981 gcgatgtcga gggtgtcttt tacgctacct tcgccctgga aagcctcccc gctttgagcc
     2041 aagctccagt ccgcccggaa cacgaccact tcgcttcctc ccacgattcc ggcgacctcg
     2101 ggcctgtcgg atatggcgaa cctcgctatc tcctggtcca ataccccgga tgcctcgaag
     2161 cccggaaacc aggtcgggta gattgcgaag tagtcagggc gcttctcctc cggcatcttc
     2221 tccagggtct catagaggct acccactccg ttgcgggttg gcagggctag atcgttggtg
     2281 gtaaggccta tcaagtccac cgtgcggtga cccccgtagt agcgcatcgc accggcatcg
     2341 tttactccca cgctggtacc gggcggcaga ttttccctta tccagtaccc cacggagacc
     2401 tgctgctcct tgatctgcag cgaattccct cctgtggtcg cggcccatac ggggaggcct
     2461 agcagcgagc acagaatcgc aaagctggcc agcgcctcgg gcagccaggt ttttctagtg
     2521 aggctcctaa gcgagtagaa ccctactacg gcgaagacga gcacgggcgg gaagaaaggg
     2581 agtaaatagc gatagtggtg ccacccccag gcgccgggaa gccccaagat cgctgtggac
     2641 gacatggcca ggacgagcgc cgtccccgaa gccacggcaa acatccggta tcgggcatcc
     2701 tccaaggcca ggtacatggt gccaagcacg cagaatagta tcgttccggg gaacaggtag
     2761 ttccctggct cgagccccgt gagcacgatg aggctcaact tcgtaagatt ctgaaacacc
     2821 gcgtccagaa cctcggtggg gtagaagact ggctcataca ggagcgactt ggcaagaacc
     2881 ccgttctgga tactgcttcc ggtagctatt gtgtagaaca tgtattgggc caccccggcg
     2941 gcgatcggca agaaggcata gagcaccgcc agggagcttc tcaacgagta gggacgggac
     3001 tttctgaggt ctctgagtag tacaaagaca acggctcctg tgattaccca agcgaaaagc
     3061 aggccctcgg gacgggtgag ggcggcgagg gcagcgagta tcggcgtgag caggaaacgc
     3121 cccgaggaga gttcgcggag aaaggctagg agcgtaccca agatcaggac cgaaaacagc
     3181 gccacctcca tgccgcttgt ggctccccag ccaaaggcgc cgttggcggc tatcaggatg
     3241 ccgctgcaga gccccgcgcg ctccccagct agcctttgtc ccagcccgta gcctaagaga
     3301 gcagagagca cgaacaacac tgttccaaag attatggcaa aacctagcag gttagcgccc
     3361 gagaacccca cgaagtgggc tgctccaagc aggagaacat agaggaagct gctggctccg
     3421 ctacttacgg gatcaccgtc gttgtagcta aaccattgcc cttcgccgat ctggcgcccg
     3481 tactgaaggt ggatgaaggt gtcgtcaagc ggtgcagaga tgttgagccc ctcatctgag
     3541 aggtcgcccc ggttgtagta cagattggtc cccacaaaca agcaacccac caataatgcc
     3601 aaagccaata taatggtgga gcccgcagaa tacttcattt tggagggtgt cctggccttt
     3661 gggcgcatag cgtcaaggac gacttggctc cggtccccta caagaggtct gtgtcctcat
     3721 tgccgccatt cactctgcac tttctcatat ctctttgtct tcctcgaaga gatgaacaca
     3781 cttcaggtcg ccatctcact tgtgctcccc atgccaatcc ctcatgacgg ccgagatgga
     3841 gaagtacaac aatagcacac gacgaccgtc taaagcagcg cacatggtga gttgttgcaa
     3901 gacactctga gaaaatagag acagagcgac ctaacaggtt gtaatgaatg ccagggagct
     3961 ctggtaggct agggccatca aggtaggcga gtgctagagg accctaggtt cggtcagcgc
     4021 acggtcaagc gcctccttcg tctcacagtc caccactacg ccggggccct atcgtaatgg
     4081 aagggacttc tagccttctc gtaagaaggc cggggagtgt tgcggccccg gcccttatga
     4141 gccgctccct ttgggctgcc taccaccaag aacacatgct gctctgttct tctgttgcgc
     4201 ccctagcgat agatcactcc ctcacaagtg tcagccgctt caatggctgc gtcaaccaca
     4261 caaaggtcgg agccaggtcc acctatggcc ccgtccatgg cgtccccgtc gttgacgcgc
     4321 agcaggtcgt gaccgccgct acccttggcc acgtcgcggt cgtttgtgta ggctcgggcg
     4381 tctaggtgat catgacctcc ctgggcgatc atcctgtctg gcaccccgtc gccgacctgt
     4441 tcaaagagga cctgatggtc acctgtagcg acgcagggga cgctgtcgca ctggatcacc
     4501 ccaccgatca tttgggcgaa ggcggtgccg gcgcttccca ccatcagcgc tacgagcacc
     4561 accgtcatag ctatcatcat cgctatcttg gtgcgcacct cttgctcctt tcctctggag
     4621 aaggggccta catcagcgct ttccccctct cggtgttacc gccgcgcgca tattaatagc
     4681 agcatctgcg gcgcgacgca tcccccaagc acactgttac ttttgccttt gtttacagaa
     4741 gtgccgggag aaaccgtccg acaaggggtt cgagcaacgc tcgaagtgtg gatctggacg
     4801 gtgtacggag gccaaaacgg gccgtagaca cctttgcctt ctggtaggtc ttgagttcga
     4861 tactactggt agatgtggcg taaaagtatt caccatggtg aatgttacct accccgtaaa
     4921 aggctgacgc tcctcctcag ctgacaccag cgaagctacg aggcccagtg tatttacttt
     4981 tcatcgcaac aagctgtgta gggctcgtct acacacgtta tagatggcgc actatcgcct
     5041 tcgcaatctc cggagcggag ttctttgcgc ccgacaggaa cgacgcagga ccgtcacgca
     5101 acgggcatca ccgagaacat gggttccggt gttgggctga ccttacaaag ggttgcggag
     5161 tgctaccggg cttcgtgcta agctgttaga ggaagcgtgg ttctttggtg tgggagaaga
     5221 gattgtttct ggacgttgac cccagagcgg acgagtcgag accagcaacg tgcgcgtgta
     5281 gacgtatgga gagtacagga ggcgcaggac ggccttcgtc agccaggctc tgagagagaa
     5341 tactcctggg ttggatccgc aactcgcctt gggccaggtc gcgcttgacc cgcgaagact
     5401 tcgctggaca tttcgtcgcc acgctgtatg cgcaggcatc cctgcagtaa ggaggtcgct
     5461 acgtggcaga ctctacaagt caggctgaac aaggcttctt ctcccggata gcctccgact
     5521 tcaccaccag agcctgggtc ctgatccccg tcggggttgg tgtcaacttc gtgggaggga
     5581 ccctggtaac cgtgctgaaa ttgccgatct tcctcgatac catcgggacg atactggtgg
     5641 cgctgctcgc cggcccatgg gtagcggccc ttacgggcct cattacgaac ctggtcctgg
     5701 tagtatcgga cccgaccttg atcgccttcg ccccagtcaa cgtggcgata ggtctgatcg
     5761 ccggtttcct ggcgctccgg ggctggttca agacctaccc gaagatcgta ttgagcggtc
     5821 tcattatcat gctcacgggc gcgatcctgt cggcccccat agtggttctt gccttcgccg
     5881 gcgtccaagg ctcgccgggt cgcagcgcac tgaccgcttt cttcctcgct actggcagca
     5941 acatctggca gagcgtgttc accacgagcg ttatcgtcga ggtagtcgac aagttgatca
     6001 gtgtcttcgt tgccgtgttc gtcgccaggg ccatcccgga acgttatcgt ccgcaaaagg
     6061 cccagcagac gctatagtgg ctcgttggcc acccgagtag atgttcgact acaggcccgg
     6121 agatagcccc gttcacctcc tcaatccggt taccaagctg gcggtagccg tagggctcac
     6181 ggtggtagtc ttcttgctgc cgaacccctg gggaccggcg ctcatcgccg tcgccctgct
     6241 cggcgttgcg gcagtggccg ggttcccagg tagggtggcg aagctcgccg ccattttgac
     6301 cctgccgttc ctggtagcgg tcgtgctcgt ccaggggatt ttctaccccg gcaacgagac
     6361 cccgctcctc gaagtgggac ccgtgacctt ctgggaggag ggggtgactt acggtctgct
     6421 ggtcttcttc cgggtgcttg tggtcgtctg cgcggtcttg atcatggtgt tctctactta
     6481 tcccaaggcg atgatggttg cctttataga caaggggttg tccccgaaac tggcctacgt
     6541 gttcatggcc tcgctccagt tcgtccccga gatgcagcgg cgggcccgcg ccataataga
     6601 ggcccagcaa gcccgcgggc tcgacctcaa ggcgaatctc tggcgccgtt tccaggctct
     6661 cttggccatg atgatcccgc tgctcgctgg ggctttgatc tccgtcgaga ccaggtcgct
     6721 ggcgctcgag gccaggggat tcagcagggg aggtatgcgc aaccacttga tcgaagtgcc
     6781 cgacaggacg accgaccgcg taattcgctg gtccatggcg gccattgtgg tgttggtcgc
     6841 aacatggcgg atagtgctgt gagcctgata gagatcgaag aactcacctt ctcctacagc
     6901 acgcagcagc acgagcccgc tctccggaac ctgagttgct ccatcgagca gggaagcttt
     6961 gtcggtatca ccggcctcgc cgaggccggc aaatccacct tctgccgcct gatcgccggc
     7021 tatataccgc acttcttcca gggggagttc tccggcaggg tgaacgtcgc cggaaaagac
     7081 accacagaga ccactatcgg agagctcgcg gagtgggttg gcttcgtctt cgagaatcct
     7141 ttcgaccagt tgaccggcgc gagcctcacg gtgctggagg aagcggcctt cgcccttgag
     7201 aacatgggtc tggcgcgcga gcagatacgc ctgcgggcag agaagagcct cacccaggta
     7261 ggcatggaag atctgaagga ccgccatccc caacagctct cagggggaca atctcaaagg
     7321 ctggcgctgg cttcgatcat cgcggtccag cctgaggtct tcatcctgga cgagcctacc
     7381 tcgcagttgg accccctcgg tgtcgaggag gtcttcgatg tcatctcgga catgcacact
     7441 agaggcaata ccctcatcgt cgtcagccag gacctcgacc acctggcgat acgctccgac
     7501 cggctgatgg ttatagacaa gggcgagata aagtgggacg gagaaccgcg ggaggtccta
     7561 ctcgaggctg cggaggccag ataccccatc ctgatccccg atgtcctcga gatcagccgt
     7621 aagctaaggg cagcgggacg catcccttcg aatcctccgc tgcccctcac cgtcgaacaa
     7681 gccgccaatg agctgtcctc gatagacact ccaggttctg ccgagaccat cgccgcggga
     7741 aggacttcgc gccattcgaa ggtggagagt agtaaagaac tcgtcttcga ggatgtacac
     7801 tacgactatc ctaccggggt gtccgcgata cgcggtgtct cgttgagtct ggacgaggga
     7861 tgcgtctgta tcgtcggaca gaacggggcc ggcaagacga ccttcgccaa gcatctcaac
     7921 ggcttgctaa ggccgactcg ggggcgcgtg ctggtccggg gcaaggacac gcgggagtac
     7981 agggtcgccg agctcgccag agaggtgggg ctggccttcc agaacccgga cgatcagctc
     8041 ttcaggagca cggtggagga agaggtgcgc ttcggaccgg acaacgtggg cgcgggtccc
     8101 gaggaagcga agcgtatggt cgctttcgca atggacctca tggggctgga agcggtccca
     8161 gagaagaagc ctcacgacct tggagtgcct gagcgtaaac gggtagcgac cgcgtccgtg
     8221 atcgccatga acaccccggt cgtggtcctg gacgaaccga cgggaggaca ggatgcggag
     8281 gggatcgaac tgctgggcca actggtaggc caactggtgc agcaagggaa gcttgtggtc
     8341 gtcgtgaccc acgacgtgat gtttgccgcc cggcacgctg acagagttat agccctctat
     8401 cagggacggg tgttgctgga cgatgacccc aggacggtct tcggcagaga agagactttg
     8461 gcccgtaccc atgtggagcc cccggccgtc acccggctcg ggaagctgct ggggctggag
     8521 gagactttac tctcgcccga agaacttctg gccgtcttcg ccccagaata acgctacagg
     8581 ctcgccgccg cgatctcctc ttctacgacg agagcaagaa cagcagccgt cgctggtgca
     8641 gcatgcaagg gtgcggcagc agggcgaaga tgcgcaggat gtactcccgc aagatatcga
     8701 gccgagagca ttctcgcagg taggccagag tcaaggtgac ttcaaggagt cacgctcgga
     8761 ccagggtcga atggccgccc tcagcatccc gccacgtccc gacctcccgg tcgacttcgt
     8821 atccgccgtc aagactatgt gctcaactgc cagtcgttga cgtggagttc gctcacctcg
     8881 acggactcgg gaagctcgag caggtagtct gggtccttct ccaggtgcga cgggcgcaga
     8941 tcgccgctgt gcgcggcctc catagcttcc atcgaggacc agtacgagat ggtggtgaac
     9001 tccgcgacgt cgccatgcat cttccggaac tgctgcatac cgaggcaacc cggcttcttc
     9061 tcgacctcga ggactccgtt ttcgtagttg taccggaggt actcgtccgc aacctccttg
     9121 cgggttcgcc cgaaccaggt tcgcgcgacg agcattctcc cgtgtactcc tgacgccaag
     9181 atttcctgct ttccggcgct gttggtctcc agatccatcc tctctcctga ggtcgtcgac
     9241 atctttgaac cgatgctacg acgctactac aatcaagtcc aactaacgat ccttacgagg
     9301 cggggggagt ctgatgccta tcgataccgc gttggtgggt gcgctgcatg tcgagtacct
     9361 cagggagcgg gacttcagtg gtgacgagca cctccaccac acgctgttcc agacttcgac
     9421 catcgatgcc ctgttggagg gaaaatacga gggcgacgtc agcttcgccg aactcgaggg
     9481 taatggagat ttcggcctgg gcacgttcga cgcgctcgac ggcgagatga tctgcctgga
     9541 cggcaccttc taccaggtaa gggcggatgg gcgggcttat gccgtcgacg gaaaggcgag
     9601 gacccctttc gccgtagtga cgctcttcga gcctgacgtc tccctgacgc tctccatacc
     9661 gatgggcttc gagggccttt gtgattacgt ggacggggtt atcggtgggg gagcagtatg
     9721 cgccgctatc cgggtggatg ggagcttcga gtgcgtcaag acgcgcagcg tgccccgcca
     9781 acgcaagccc tacccacccc tcgtagaggt cgtcaaagac cagcccacct tcgagcttca
     9841 cgatgtctcg ggtagtctcg tcggcttccg gtttcccgac tacgcgcagg gtttgaatgt
     9901 ctccggctac cacttccact tcattaccgc ggacaggagt gccggtggtc acgtgctcgg
     9961 atgtaagctg gttcgtggcg agctctacat cgaccacgaa ggggacctca ggctcgagct
    10021 tccaccagac gtgggcttgc caacatcaga ccagtcggcg gcgaaagggg agacgttaga
    10081 ccgcatcgag agggagtagc gtacggcaca gccctttcgt cggtaacggg gacctcctcc
    10141 ggaacccgaa ggttataccc cacgttcatc tggcagcggc cggcggccca agcattgtgg
    10201 tactttcgga agtggtacgc tgttctgatg cgatctacga gttcgcgaac cctggaggat
    10261 tgggaagccc tacccatcgc ggccccactc ggaagcgcca gagaagcggc aggaataaga
    10321 cgggcctgga ctcccatacg cgttccctgg cattggcagc tcgaagacgc cttcgcaact
    10381 tatgagggga tcgtattgta ccggtgcagg ttcgcacagc aaaggcccac tgaggggacg
    10441 atgctctccc tcaggttcga gggcgtttac tacgcggcgc gggtctggct caacggcgtc
    10501 tacctgggcg aacatgaagg atacttctcc gcgttcgagt tcgatatcac cgacgtggtc
    10561 gtcagcgccg gtgagaacga actgctcgta gaggtttgca gccccgaaga atcagatgag
    10621 aacgaccgta agaccgtcgg aggcgtatgg gcccgctggg acggtatggc cccccacatc
    10681 aacccaggtg gcatcttcag agaggttaca gtcgttgtag atggagaggt gcgcatccgc
    10741 tcgctcgggg ctgcggccga ccactccggg cgtggcgtgg cgcatgtaga cctttactcc
    10801 aggtataacg cgcgcgccag gctggttgga cgggtacgcc cgctgggctt ttcggccccg
    10861 gatgtcgagt tcgagaggga ggtccaggtc gaggggggtg agaaccgctt ggagatcggc
    10921 ttccgcgtgc ctgacgtaag ggtatggtcg acgtgggaca ggggcgaaca gccactctac
    10981 gagctggtgc tatggtgtgc aggggcagaa gagcgaacgc gtttcggtgc cagtaccgtg
    11041 gagttgcggg actggaaagt ctacctcaac ggggagcgta tcttcctgcg cgggatcaac
    11101 tacctgccct cggacgcata ccctgcaagg gcaaccgagg agcttctgcg cgcggacgcc
    11161 tcactggtgc gtgaagccgg catgaacgcc gtcagggtcc acgcccacgt gtcggaccga
    11221 gaattctacg aggcctgcga cgagctcggg cttctcgtgg ttcaggattt tcctctgcag
    11281 tggacccaca ggcggagcat actcgggccc gccgtcaccc aggcagggga gatggcgcga
    11341 atcctgcgcg gcaatgcttc cgtgggaatc tatctggcgc acgacgaacc tttctttgtg
    11401 gcgccccccg agaaatggag ccctctcggt ctcctgcgca ccgccgccga ggtgctctcc
    11461 ccgcgatgga tgctctggca gcgtcgggtt ctcgaccctg cggtggtgcg tgccatctcg
    11521 gaggaggacg actccagacc cgtaattgac gcggcgggcc atcccctcac gacaaaccac
    11581 ctctacttcg gctggtacta cgggaaattc agggacctgg agcgggtcgt gaaggtcttc
    11641 ccaggattcc cgcggctgcc gacggagtac ggggcgcagg cgctgcccga tcccgaatct
    11701 ctcgaagaga tctggccctc ggggacgcgg cccgactgga gcgtcctctc cctgaactac
    11761 cgcctccagg tgcagcgcat aacccgctac gtcccctgga ggggagatcg tgcaactttc
    11821 ttgcgcgaga gccagcgtta tcaggccgag gtcctgaaac acgccacgga gctattccgg
    11881 cgacgcaagt acaggccgac gggggggacc ttcgccttta tgctcaacga tcccgctcca
    11941 gcagtgtcct ggtccgtcgt ggactggcgg cgcaggccca agatagccta cgaaacgctc
    12001 cttgatgcga tgaggccggt cctgatctgt gccgaatacc cgaaggagcg ctacagcgtc
    12061 ggcgacagga tctccctgcc tcttttcgtt atcaatgacc tttcacggga gttggggata
    12121 gtagattgga gctgggaact tttcctaggg ggatcgagcg cggcccacgg tggaggagag
    12181 acaacagtac cgaaagattc tgtagtaagg atcggggaag cggtggctac gctttctact
    12241 tcggggccag ccgtactcag gctaacgctt ttcggggagg agggaccact ctccaacgag
    12301 tacgagttcg tagtcagcca ggctggtgct cgcgcgcatc gaggtccatc agctcgggga
    12361 gggtgagaaa gccgtacccg ctcgccttga ggccgctgat gatgtcggag acggccgcta
    12421 cggtgtggga acggtcggcc ttggggaaag cgtctgtgtc ctggccgtcg tggagcaagg
    12481 ttacaccgcc tggaccaacg ccatgcaata tgcggcgttg gatcatgtcc ttaggaggac
    12541 ggtaccaatc ccaggccatt accgaccagc ccacacacct catccctagc tcctcggcca
    12601 cggagatcac ctgtggacca taccggccgt gggggacgcg gaagagttgc ggtagagtgc
    12661 ccgtggctcc gtggatggcc tcctggccac ggagcatctc ttccctgacc atcctaggcc
    12721 ggctccacgc gaggtgcggg tgggtgaacg tgtggttgcc gacctcgtgt tgggaagcga
    12781 cttcggcggc gaggttcggc tcgcgcatga cattcttgcc tacctggaag aaggttgccc
    12841 tgatgccttc gcggttcagg atctcgagca ggtgccccgt gtagggatga caagggccgt
    12901 cgtcgaaggt cagggcgacc acccgctcat ttgtacgagc gtgccagaag acagggccgt
    12961 agagccagga gcggggcgtg agccattcgt aggccataaa cccgccggcc gccatcgagg
    13021 cccctgccgc ggccagggta ggtacggaac gtaccgggcg gcctgttgcg gtccgtagac
    13081 ccgcgagggc cgcgccacag acggcggtgg tggcgacgac agtccaggcg cagggatgtc
    13141 tcacggcagg ccagccagta caagggaggc ggccgaacgc gccgagtgtg gcctgtgggc
    13201 ggcttccgcc gcctggacca tattgtgcct gtgatccgcg ctggtcagga tcttgagcgc
    13261 ctggcccagg agctgcgacg tatcacggac catgaccccg gcaccgtagc ggactatcgc
    13321 cgccgcgttg tcctcttcct gaccggggat aggatggtag agaacctgag gcagcctcga
    13381 agcgagggcc tctgtgcagc tcatgccgcc cggtttggtg acgagcacag cgcctgcggc
    13441 catcagctcc cttacccgat ccgtgaaagc aaggaaggtg gccttgtgtc ctccacgttc
    13501 cgcgaggcgc tccagccttg tacgggaacg ttcgttagac ccgcagagca cgacgaggtg
    13561 catagcctca ctggcctgaa gcagcaacga tacggactct tccaggtccc ctccgccgag
    13621 gcctccgccc ataacgagca ccacgggaag gtcatcatcg tccaggttca ggatctccct
    13681 gcgggccggg ccagggtcgg ccggtgcttc gaagatgggg tctataggga tacctgtcgg
    13741 atggatctgc tcctcggaca cgccgagctt cgccacgtct tcggccgcca catcgtcggc
    13801 gacgacgtag agatctaggt tcaaaggcac cccgatcggg tgcggcacgt agtccgtcag
    13861 cacgacggcg ctgaacatcc ccgggttctg ctctttgtat gatccgagaa ccccggcacc
    13921 gtagaagtga ggggctacaa gggccttcgc gtcggtgctc gacaccaggc gctccagggt
    13981 cttgagtcct gccgaccgca ggtagttcac gtacgagcct ggctggcccg tgcccgaaga
    14041 ctcatagagc ttcccgtacg ccgtgggcca cagctttagg gcctccagat acgcgtctcg
    14101 cagcagacgc tcgacgccag gcgagacttc gtgggggaag tccacgatcg agcaggtcct
    14161 ctcggggtcg aggatatcca caccacgaac cacggccgcg cttgcccgca tgtggccgct
    14221 tcctat
//
